LOCUS       DAOPUE010000001        61651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_434_length_61651_cov_10.687204, whole genome shotgun sequence.
ACCESSION   DAOPUE010000001 DAOPUE010000000
VERSION     DAOPUE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 61651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61651
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_434_length_61651_cov_10.687204"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..944
                     /locus_tag="PKH42_00005"
     CDS             18..944
                     /locus_tag="PKH42_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA18835.1"
                     /translation="MDSKLALYIVKRLTLAVITIFLIITITFFVMHEVPGGPFLSEKA
                     PSAAVTAALEAKYGLDKPKSEQYVTYLKGIMKGDFGPSIKQRGREVTAVIMQGFKVSF
                     KLGGTAAVIALCIGTVLGSVAAIFHNKTIDKIIMVVTTGLVAMPSFIIATLLLLVFCV
                     WMGIFPANGNEPGGLILPIISLSLYPMSYTTRLTRSSMLDVLSQDYIRTARAKGVKPA
                     KVIFKHALRNAVTPVVTYAGPMFAYIITGSLVVEKIFAVPGLGKEFVSSIINRDYTMI
                     MGTTIFLGTLIIIMMLVSDILYKVFDPRIELG"
     gene            963..2042
                     /locus_tag="PKH42_00010"
     CDS             963..2042
                     /locus_tag="PKH42_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA18836.1"
                     /translation="MNNIDEMPKKNPLSFQLNLKDFEKATEDEKAQQVTMSESITFFK
                     DGMRRLRKNKIAMTCLTVLILIALIVTFVPMVYPYTYEQQLGVAQGKRIDKTYNNLKP
                     FEYGETELERIANGEKIFPHIFGTDAAGRDYAIRVIYGGRISLLVGFFAAIIVLIIGV
                     VYGSIAGYFGGKVDLFMMRIVDIIYSLPDMLIIILLSVVLNQVLQPKIEGTSLAKVGT
                     DMISIFIVFALLYWVSMARLVRGQILSIKQNEYILAAKSVGSSPWRIIKKHLLPNSMS
                     VIIISTALQIPSAIFTESYLSFLGLGVSIPMPSLGSLASDALTGIYSYPYRLIIPAIA
                     IWLIVLSFNLLGDGLRDAFDPKLKK"
     gene            2053..3075
                     /locus_tag="PKH42_00015"
     CDS             2053..3075
                     /locus_tag="PKH42_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA18837.1"
                     /translation="MSLLQVKDLYTSFFTSSGEVKAVNGVSFNLEEGKVLGIVGESGS
                     GKSVTAYSILQILTNPGRIVSGSIKLKGQELVGCSKETMQKIRGNQISIIFQDPMTSL
                     NPVFTIGNQLMEAILLHTNRNKEEAKARAIEMLKLVGVNEPEKRIEQYPHEFSGGMRQ
                     RVMIAMALACEPDILIADEPTTALDVTIQAQILDLMKELQKKLGMAIILITHDLGVVA
                     EMCDDIIVMYAGQISERGTADDIFYNPKHEYTKGLLKSIPTVQDEKQRLKPIGGNPVD
                     LLNMPAGCPFAPRCDNAMKICLTVNPVEMTIEGKHTSRCWMNVREGIENETLTIKQVE
                     EMYHGK"
     gene            3065..4033
                     /locus_tag="PKH42_00020"
     CDS             3065..4033
                     /locus_tag="PKH42_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA18838.1"
                     /translation="MENNNNLLEVRNLKQYFDIPVGLLKSKPLKAVDDVSFSIRKGET
                     LGLVGESGCGKTTVGRSILQLYKPTGGEIIFKGKKIESKESIKQLRQNAQMVFQDPYS
                     SLNPRMTVGDIVGEPLDVMNMYKSRKERDKRVNELMEIVGLSSEHATRYAHEFSGGQR
                     QRIGIARALAVEPEFIVCDEPVSALDVSIQAQIINKFEDLQEQLGLTYLFIAHDLLVV
                     KHISNRIAVMYLGRIMELAPSDELYNHPLHPYTISLMSAVPIPDPSYARSHQRILLEG
                     DVPSPLNMPSGCPFRTRCKRATKECAEIRPELREVAPEHLVACIHV"
     gene            4247..6091
                     /locus_tag="PKH42_00025"
     CDS             4247..6091
                     /locus_tag="PKH42_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA18839.1"
                     /translation="MKKILSLILVLAMVFSLAACGNNQPPAEPETPETPETPETPEEP
                     GIADSLTLHVGAYPDTIDPALNSAVDGATYIIHAFAGLVGYQQKEDGSLELYPDLAKE
                     LPVGKERDDGKVEYVYELKDGLKWSDGSDLTAHDFVYAWNRAANPETAADYGYMFEVV
                     DGYAEMTEMDDEGNLVNPDAKLNVTASEDGKTLTVVLPVDVPYFNELVAFPTYMPVKA
                     EVAEGNDAWATAADTYIGNGPYKVVEFSQSQLIMQKNEYYHDADRVITNELVFAFNDD
                     DTSTLANFQNGSYLFIDQVPNDEIKSLQEQYPDEFFIEGQLGTYYVSFNVNDENFKAF
                     GEADKAKIRKALGLMIDRTYIVEEIGQAGQVPAAGFVPMGLTEPDGKTEYIAKNGPKG
                     DGAGYYSVDPDDYEKNCEEAVALLREVAESTGLYTVDENGVVSKEFPTIAYLTNEGTG
                     HEAIAINLQATFAQYGINMTIEKQEWATFLNTRKNGDYSVARNGWLGDYNDPISFLDM
                     WITSSGNNDSQFGKGEHATYAGYTYKGEGGKTWAESYDKIIAEVKASKDSEERFALMH
                     EAENILMETGAICPIYYYTDIYMKSTDLQGAFASPLGYKYFMYSYVEE"
     gene            6308..7141
                     /locus_tag="PKH42_00030"
     CDS             6308..7141
                     /locus_tag="PKH42_00030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOA18840.1"
                     /translation="MKYKMVVLDLDGTLLNSEKTISDKNAHILIELHKRGIHIVIATG
                     RNYYMAKTLIERLKFTEPVILANNGAVVRHYVNDEFIESNYLDFSTFERIYELGLKHD
                     LNPIIHVDEYNQGYDIIYECENYEEVYLGYIKKDYKRTRCKKFKTEEINNILSVCYFN
                     EYQKLLKLKSEIEKIPGKFNIILNQNISNWSLLELLNINGCKWCALKKYIDRLGIDAN
                     QVIAIGDDNNDIELIKNSGLGIAMKNATDELKKAADSISRFSNDESGVYYELSEIFKI
                     K"
     gene            7220..7411
                     /locus_tag="PKH42_00035"
     CDS             7220..7411
                     /locus_tag="PKH42_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOA18841.1"
                     /translation="MRIITAIASILIIIGALNWGLYGLSKIDIVENLFGGWKSKFGRI
                     IYLLIGAAGLYSLLYVIFA"
     gene            complement(7597..7815)
                     /locus_tag="PKH42_00040"
     CDS             complement(7597..7815)
                     /locus_tag="PKH42_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HOA18842.1"
                     /translation="MRIKEGIVPTILGTVVTATGVALKNTNLPESYRNGLIGFGLANI
                     AIGSAELVSSNWQHSRIMNRMTKMAPFK"
     gene            7961..8875
                     /locus_tag="PKH42_00045"
     CDS             7961..8875
                     /locus_tag="PKH42_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589008.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOA18843.1"
                     /translation="MKLYNKYSDYLKLKYGEKVYKLPVNIPCTCPNRDGTLGYGGCTF
                     CADVGTGFEMLDNSLSVKEQLNKNMDYISKKYKSGKFIAYFQNYTNTYMDIERFKNYI
                     NDAIMDNIVEIAISTRPDCISDEILEFLNEIKKQNVNITIELGLQTVNYHTLKNINRG
                     HTLAEFIDAVLRIKRYNFEICTHIILNLPGDTILDTVETAKILSVLKINQVKIHSLYI
                     MENTEMGRAYKNNEITLISKDEYVERVIVFLEHLDVDITVQRLIGRAPEKDSLFVNWG
                     MSWWKINDDILYKMKSENRYQGSKNKFV"
     gene            8945..9379
                     /locus_tag="PKH42_00050"
     CDS             8945..9379
                     /locus_tag="PKH42_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18844.1"
                     /translation="MNRSVIIMVQLVCGHSGTGKTRRMIEMANNAIDKIHGSMVFLEA
                     SNRHIFDLDYRIRYINTKEFGLTNDEEFQGFVCGVIATDYDIEQVYIDGLYKIAKSGK
                     ERLVPLIERLDYLSKTFDVNFIISVNHDIEDIPEGLRDRVID"
     gene            9563..10117
                     /locus_tag="PKH42_00055"
     CDS             9563..10117
                     /locus_tag="PKH42_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18845.1"
                     /translation="MNYFKSKNSIFDDFFEHRDMLIIQHMNGDINKKEYLELNYKYML
                     EKNIKPFQRIDSFEKGMYNYQYYNMMAKYHRMIAQEIKDKGKHISYYNKYIKEADFYY
                     NEKDKTIFRLLRFLQFENIESYFIKMESSLLEGKLYEIVLKDFEYAVLHSKSLWLLDV
                     LKKEKVFTEKRKKSVIDYYVNQRY"
     assembly_gap    10146..10245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10251..10712)
                     /locus_tag="PKH42_00060"
     CDS             complement(10251..10712)
                     /locus_tag="PKH42_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18846.1"
                     /translation="MKRIINAIRDFIYNITDYGLIITVIVVMTAILVWRFDLLFSRGI
                     EKEIIKDLPIINEPEVNEPIAEEPDPEDEEPEPEDETPTGLIAAISIPQGSFPSQIAE
                     ILLNNNLIKSKDEFLTRCVELDLDTRLQSGTFNIEVGTPLDDVIKIIANAY"
     gene            10914..11822
                     /locus_tag="PKH42_00065"
     CDS             10914..11822
                     /locus_tag="PKH42_00065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA18847.1"
                     /translation="MKEIKITKNEEGQRLDRFLKKYLNKANQSFIHKMIRKKNIKVNG
                     LKAGPDIVLKTDDTVQLYLSDETIDKFRDKKTLQKTDIHFDIVYEDDNILVVNKPIGL
                     STQPDKTGKKNLVDEIKVYLDAKEENISFTFKPAVCNRLDKNTSGLVIAAKNYDALKQ
                     TNKAIRERKIRKYYMAKVHGIIKDDLELKDYLIKKDNKNMVKIINEYRENSKSVITYA
                     HPLKREGKYTWLEVEIETGRAHQIRAHLASIGHPIAGDKKYGKKDNEKYQVLHAYKLL
                     LDGFEGNLSYLNGMEIVSDISERKIL"
     gene            11833..13485
                     /locus_tag="PKH42_00070"
     CDS             11833..13485
                     /locus_tag="PKH42_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOA18848.1"
                     /translation="MKIKLANTDEVIEVYENETLYRILKRVYKNDYFKYLGAKVNNKL
                     KGLCSKDFKENDRIEFIDITNPDGHRIYVRTLSLIYVKACKDIYNDIDVSINHSLNKG
                     LYTELQYKHLVTKKHLDAIKNKMQEIIDAKKNITTYFLSVDKAREIFSAQGMMDKVEM
                     LKFWNKDTIRVYELDGYYDTFYGYVAPNTGFVNKFDLRLFYPGIILNYPTREYNFELP
                     EYIEQKKLSKVFKEAEDWGEILDVAYVGALNKKIVNNEIYDMIRINEALHEKKIAYIA
                     DEITNDKNIKIVLIAGPSSSGKTTLAQRLSIQLRVNGKKTYAVSLDDYFVDRHLTPKN
                     EKGEYDFETIKALDLDLFNEQLIDLMAGEEVQIPVYNFKEGSREYTREPVRLTNDHII
                     IIEGIHGLNDELTANIPQVNKFKIYISALTQLNIDRHNRISTSDLRLLRRIVRDYTHR
                     GNNAIKTMELWDNVIKGTEKYIFPYQENADAIFNSALVYELCVLKKYAEPIIMEIDED
                     SCFYSERQRLLKFLSYFLPIEDESAIPNTSILREFIGESCFR"
     gene            complement(13548..14699)
                     /gene="rodA"
                     /locus_tag="PKH42_00075"
     CDS             complement(13548..14699)
                     /gene="rodA"
                     /locus_tag="PKH42_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOA18849.1"
                     /translation="MFKKESKLKRFDFILLITTILLCIYGFIAINSATMSKATGSEPF
                     LKTQIIAFGLGMVALFVLIFIDYDIYGSLYIPIYVMTNILLLYTLINPVEASEWGDVR
                     SWIAIGPIVFQPSEIAKFGIIISLSKFIDIHKDNINQPVVLLKIILFAFLPIALILMQ
                     PDLGTAVVFIFFVAIMIYISGIDRKYVLSVMIIGLILIIAGLSIFFQIMEDYTLNEDY
                     RLDRIVTFFYPELDPDDTGYQVIQSKTAIGSGMLYGRGLYKGVQNQLGYLPTKETDFI
                     FAVIGEELGLVGGLVLLFMYAVLFYRLIRIAKNAANMFGSLIVTGITAMQLFHIFENI
                     GMTMGLTPVTGIPLPFISYGGTFMLVNMISIGLCLSVGMKRGKIDLNEF"
     gene            14868..15368
                     /gene="ruvC"
                     /locus_tag="PKH42_00080"
     CDS             14868..15368
                     /gene="ruvC"
                     /locus_tag="PKH42_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOA18850.1"
                     /translation="MLILGIDPGLAISGYGILNYSGNKFKVIEYGCIFTESCEEFPKR
                     LKHIYESYISIIDMYKPEAVAIEELFYNKNAKTAMAIAQARGVHLLAAENKNVPLYEY
                     TPLQIKQGIVGYGRAEKKQVQEMVRVILKLNEIPKPDDAADGLAAAICHAHSLKFAKE
                     FKINKY"
     gene            15377..15961
                     /gene="ruvA"
                     /locus_tag="PKH42_00085"
     CDS             15377..15961
                     /gene="ruvA"
                     /locus_tag="PKH42_00085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOA18851.1"
                     /translation="MISYIKGEVVKKGIDYLILDNNNIGYYINTSFSTLEKISEKEKI
                     IILTYMHIREDIIALYGFLTSDEIDLFKKLISVNGIGPRAGLSVLSTYEVNTVKEIIL
                     KEDVSGLSKVPGIGKKTASKIILELKDKVGTVEELKGSEIVVSSEMSDVIEALTSLGF
                     NYAEVKRTLGNMELSGKSENDIIKEALKNMNRQG"
     gene            15963..17018
                     /gene="ruvB"
                     /locus_tag="PKH42_00090"
     CDS             15963..17018
                     /gene="ruvB"
                     /locus_tag="PKH42_00090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOA18852.1"
                     /translation="MFERENEIVARTFNSCEEELELSLRPQKLPQFIGQNKVKEVLNI
                     FIESAKIRNQPLDHVLLSGPPGLGKTTLANIISNELGVNIRVTSGPAIERAGDLAAIL
                     TNLNENDVLFIDEIHRLNKTVEEIMYPALEDFALDIIIGKGPSARSIRLDLPKFTLIG
                     ATTRSGMLASPLRDRFGVMCNLDFYEPEDLKEIVLRSASIMNIEVEEKGAEEIAKRSR
                     GTPRIANRLLKRVRDYAIVKGEGIIDLKTADEALKMLEIDEIGLDKLDRRILNTIIDF
                     YKGGPVGIDTLAASIGEEKGTIEDVYEPYLLQIGYLNRTPRGRVVSESGYRHVKKIRK
                     IQDQINILEDDYEEEFD"
     gene            16999..18681
                     /locus_tag="PKH42_00095"
     CDS             16999..18681
                     /locus_tag="PKH42_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOA18853.1"
                     /translation="MKKNLIRLFIITALLTFLFPTTAFGKNKDDIYVRVRIRYPRLFN
                     EQVSFEGFDEIVLYDDEDELFCLNNSLTLRIDTYYSSNYYLSDKSQGTYGPYHAVLQD
                     EIYSSYEEAYLIAEELNEEFKVEFYPYLIEDGYIIAGGNFADKSSSQDLAEELNDEGY
                     IAESMNGNLENIIAYNKKNIPVFMYKKDMPVYFSSYNQDEEYEMIKVDNRAYRGRIRL
                     NIIEDFKLLTINHVELENYLYGVVPNEIPSSWGIEALKAQALAARTYAVYNIKPRQIY
                     DMEDNQNSQVYMGYDYEKTSTNRAVDETRGEMIYYDGELIQAFYHSTSGGSTENSENV
                     WYEKLPYLRGVNDEFSNNSGSPHTTWQTTYYKEDIIRNLNLDGNDVDELYGIQIKKVS
                     ENNRVVECIFLTDAGEISYKKENARLLLGLKSSWFSIVNGNIFCFITEYFSLDENRKD
                     ETPPSRGVLDLITEEETEETRNEIEFETGSIAGKHAITDDGIKKINREKLSFISSNGV
                     TTVDANSEQYNFEGRGWGHGIGMSQYGAKQMAEEGYTYEEIIKHYYTGVTVK"
     gene            18678..19712
                     /gene="queA"
                     /locus_tag="PKH42_00100"
     CDS             18678..19712
                     /gene="queA"
                     /locus_tag="PKH42_00100"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOA18854.1"
                     /translation="MTNFKTSDFYYELPERLIAQTPIKERDHSRLLVLDKKSGKIEHK
                     HFFNLVEYLNAGDCLVINDTKVLPARLFGNKKDTQTLIEILLLKRIDDKKWEILVKPG
                     KKARTGTEIVFDEKLLTGKIIDILEEGNRIIEFEYEGIFEEILDRLGEMPLPPYIHEK
                     LKDKDRYQTIYAKHLGSAAAPTAGLHFNDKLLEEIENKDVNIARLTLHVGLGTFRPVK
                     TEYINEHIMHSEYYELNESEANKINLAKSSGHKVITVGTTSVRTLETVADDEGIVNKS
                     KGWTNIFIYPGYKFKVVDSLVTNFHLPESTLIMLVSALAGKENIMKAYREAVEKEYRF
                     FSFGDAMFIK"
     gene            19720..20829
                     /gene="tgt"
                     /locus_tag="PKH42_00105"
     CDS             19720..20829
                     /gene="tgt"
                     /locus_tag="PKH42_00105"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOA18855.1"
                     /translation="MFKFELIKESKECKGRLGRIYTPHGIIETPVFMPVGTKGTVKAM
                     TPEELKQLRAEIILGNTYHLYLRPGHELIREAGGLHKFMNWDGPILTDSGGFQVFSLE
                     NLRKIREEGVEFRSHIDGSKHFISPEVSMEIQNYLGSDIMMAFDECAPYPADYEYVKK
                     SMERTSRWAKRCKDYHKNTKSQALFGIVQGGMYKDLRIESAKQITDIEFPGYALGGLS
                     VGEPRDLMCEVMDYTVELLPKDKPRYLMGVGSPDYLFEAVERGIDMADCVLPTRMARH
                     GAFLTSKGQLTIKNAKYKNDFSPIDEECSCYTCRNYSRAYIRHLFGEKEILGARLASI
                     HNLFFLINLMKGIRISIAEDNFMKFKREFYENYGY"
     gene            20850..21194
                     /gene="yajC"
                     /locus_tag="PKH42_00110"
     CDS             20850..21194
                     /gene="yajC"
                     /locus_tag="PKH42_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOA18856.1"
                     /translation="MPQEYSGILMLVVFFAIMYFTIIRPQKKREKETKAMRDSLATGD
                     EVITIGGIHGKVVKINDEIVTLEMPFGKQRIEFSKWAIGSVTKKGKEKAAKEEVVKEE
                     IEETVDDKEDDK"
     gene            complement(21301..23982)
                     /locus_tag="PKH42_00115"
     CDS             complement(21301..23982)
                     /locus_tag="PKH42_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOA18857.1"
                     /translation="MYYRKSITETCEELNTAQSTGLTQEEAKKRLLSDGPNALVEKKA
                     KTKLQMFLAQLKDTLIYILFAAAAISIALNEITDAIIILLVVLLNAVIGMVQESKAEA
                     ALEALKNLSSPYAMVRREGRVMEIPASELVVGDLVILEAGRIIPADLRLIQSVNLKIE
                     ESALTGESVPVDKNADMVADKEVGIGDRLNMAFSSTSVSYGRGEGVVVYTGMDTEIGK
                     IAAMISESTEELTPLQKRLNDLGKVLGIVAVVIVVAMFGIAVLQGRDIIEMFITAIAL
                     AVAAVPEGLTAVVTIVLALGVTKMVRVNTIVRKLPAVETLGAVSIVCSDKTGTLTQNK
                     MTVTKVYLDGEIKNTDDLSYENNKIFLDGFILCNDASIANNTRIGDPTELALLDMGDK
                     INLSREGLEKTNPRINEQSFDSARKLMTTVHRDQDGKVMSYTKGAMDILLNRCNKIYI
                     NGETKDITNEHIEEINRAATEMAKGALRVLALGIKEDDDSASEEDLTFVGLVGMIDPP
                     RPEAKDSVKTLRSAGIRTIMITGDHKDTALAIAKELGIAEDESQCITGSELNEMTQEQ
                     LNEKVCDIRVFARVSPEHKVMIVKAFKSNGSIVSMTGDGVNDAPSLKSADIGVAMGIT
                     GTDVAKGASDMVLTDDNFATIEKAVAEGRGIYNNIKKTVLFLLSSNFGEVISMFSSIA
                     AGLIAPLQSIHILWVNLITDSLPALALGVDPKSKEIMNEKPRDPNESLFAKGGLIFTV
                     YNGIMIASLTLAAFLWSPVIHLNEAGIPVTLGNIRDLFNGVLSIPGVDLPDIIRHAQT
                     YAFTTLGVSQLFHAIGMRNYNKSLFQMSHTDNLAMIGAFSLGLLLQILVTEVHFLTEV
                     FETSQLSLKEWANLILVSAVPLLSHEVIVMGKKIFKK"
     gene            24253..24621
                     /locus_tag="PKH42_00120"
     CDS             24253..24621
                     /locus_tag="PKH42_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOA18858.1"
                     /translation="MKLGKLFKYSLFLFILSLIIYFILTVYIYYFSNNPGFEIVYSFI
                     VPICIFAVSMLYSRSVHEKGLLRGIEIWIVYFAMVLLIKVLFRYPSEIRILYNGVILI
                     MSILGGIIGVNMKNKSIKNK"
     gene            24706..24846
                     /gene="scfA"
                     /locus_tag="PKH42_00125"
     CDS             24706..24846
                     /gene="scfA"
                     /locus_tag="PKH42_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOA18859.1"
                     /translation="MKYIKTLNNYKLTDNLSAKGCGECQTSCQSACKTSCTVGNQKCE
                     KE"
     gene            24987..26321
                     /gene="scfB"
                     /locus_tag="PKH42_00130"
     CDS             24987..26321
                     /gene="scfB"
                     /locus_tag="PKH42_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOA18860.1"
                     /translation="MIHVFNIKDLYFATDTSTGLVHAVDKIVFDLLMNEKFKDENKYS
                     ELYNEYGESTVREALSEIQYLIDNKMLYTEDIKYVNTIKPVIKAMCLNMTHDCNLRCE
                     YCFASQGTYNGEKAFLSFETGKKAFDYLVKNSGNRKNLEVDFFGGEPLMNFDVIKKLV
                     DYGRSLEKEYNKHFRFTVTTNGVLLDEEKMDYINENMDNVVLSIDGRKETNDRMRKTI
                     NKKGSYDLIVDNYKRFISKRGSKDYFARGTYTSNNLDFSEDVKHMRELGFDKISVEPV
                     VAKDEEKYALKKEHVDILKKEYEKLAEYYIESYKSKDRRFQFFHFNIELEGGPCIYKR
                     SIGCGAGTEYVAVTPSGDLYPCHQFVGNEEFIIGNVEEGITNRALADKFKNVSVNDKP
                     ACRDCWAKYFCSGGCHANAYNFNKDFTVPYDVGCELEKKRIECAIYIKAKIN"
     gene            26439..26984
                     /locus_tag="PKH42_00135"
     CDS             26439..26984
                     /locus_tag="PKH42_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HOA18861.1"
                     /translation="MQIGFIVILIIAIFVAIFAIQNGTPVPVDLFFARYEMPLAVIMM
                     ICLILGAVVVLILGTTRQFKKRSEYNELKNKLKTLEGEKAKHEIDMKALESEKAQFQT
                     NIEALETEKQRLQNEIADFSKKVTLLEDKIKLQDEELVAKNQEIQILKAQNIEKIEGD
                     TVITDEGAAQIDNETSEVESQ"
     gene            26956..28869
                     /gene="recJ"
                     /locus_tag="PKH42_00140"
     CDS             26956..28869
                     /gene="recJ"
                     /locus_tag="PKH42_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOA18862.1"
                     /translation="MKPVKLKVNEYNNDEIERISKRFNLSKTSAKILLNRNIKSFDDI
                     EQFLEPNFKYLERAENYKDLHKGCKRVIEAINNNDRILIYGDYDVDGVTSISQFVVFL
                     KKAGAYVNYYVPERESEGYGISSDFVNSVKSGEINFELLITVDCGIAEIEKIDEITKL
                     DKDVIIIDHHESREELPNAYAIINPKQKDCPSENKHLCAAGLSFKFLINLNKFLKIDN
                     IEDVLLEYACLGTIADIVDLVKDNRIIASQGLKKINKTKIIGLKKLIEVSGINGEIKS
                     YHIGFILAPRINASGRMDTAKKAIKLMLTKDEEEAEKLASELDSLNNARKEAENIIYK
                     EAIKKIESDFLYKNNVMVVYGDNWHEGVLGIVASRLTEKYYKPCVVISVKDGIGKGSA
                     RSVEHVDIFESFKHTDIYLEKYGGHKLAAGLTILEKNINSFTNELNNYIGSTTDDEYN
                     QIRADAILNISDINLRLYDEINRFEPFGSGNPKPLLALRDVSIKNIRKVGKEGKHISF
                     MLSDGDRHIPVIGFCKIGILEKILTMPQSYIVSLSENIYNGSRSLQLFLHDVEEHEEF
                     DYKIDVNKLKALEFLINKTKSKIIKTDLFILVEKLNKRYNTKITAEEIICMLKKADNI
                     QFALKNDILYIKK"
     gene            28886..29404
                     /locus_tag="PKH42_00145"
     CDS             28886..29404
                     /locus_tag="PKH42_00145"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOA18863.1"
                     /translation="MDLKEKIRVIEDFPAKGISFKDITTLLKDSEALHECVKEMAERF
                     KDVHVDIIAGPESRGFIFATPLAYLLKTGFVPVRKPGKLPAETIKYEYQLEYGTDSLE
                     IHKDAIKKGQKVLIIDDLLATGGTMYATAKLVEKLGGEVAGLGFLIELKDLKGREKLK
                     EYRVESLITYDK"
     gene            29487..31661
                     /locus_tag="PKH42_00150"
     CDS             29487..31661
                     /locus_tag="PKH42_00150"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOA18864.1"
                     /translation="MLGNIISQIESYNPNADLGIVAKAYNFAEMAHSNAQTRFSGERY
                     FIHPYNVALILADLHMDVQTVCAGLLHDVVEDTGITFDEIKREFGEEIANMVDGVTKL
                     SKVKYRNKEERQAESLRKMIVAMSKDIRVVIIKLADRLHNIRTLQYMPKEKQYQKAME
                     TIEIYAPIANRLGIASIKWELEDLSLKYIDPEGYEDLLKKVQEKSEKRKDYISHIIDI
                     INKKLQESGMEADIKGRPKSLYSIYKKMYYKNRLFEQIYDLIAIRIIVENIKDCYGAL
                     GIVHTLWKPVPGRFKDYIAMPKPNMYQSLHTTVIGPEGEPFEIQIRTLEMHRTAEYGI
                     AAHWKYKEGIDDKTKYEEKLNWLRQMLEWQQETNDPQEFMEALKIDLYTDEVFVFTPK
                     GEVINLPTGSTPIDFAYRVHTAVGNKCVGAKVNGRIVPLDAKLKTGDQVEILTSQSSV
                     GPSRDWLKIAASSQAKSKIRKFFKDRDKDFNVEKGKELVEREIKKQGYQVADLLKDDW
                     IKTVADKYNMSAASNLFAAVGSGGITESQIVNRLKLIYLEKNKDKLELDRIENLEKSL
                     AKSPQRERHTSGVIIKGIDNIKVRFSKCCNPVPGDAIVGYITRGRGVSIHRADCTNIH
                     DLSDSDDQRFIEVQWDNKEKATFISELQIKALDRPKLLQDITSLYVDAKLNAVSLNLR
                     INKEKVAIVDIGFEINDIKQIEDLIKKMKKLDGVLEVYRNKK"
     gene            31684..>31862
                     /locus_tag="PKH42_00155"
     CDS             31684..>31862
                     /locus_tag="PKH42_00155"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOA18865.1"
                     /translation="MRAVVQRVKSAHVKVKDKIIGSIGQGILQFLGVEETDQETDLEY
                     MCEKIPNLRIFEDENG"
     assembly_gap    31863..31962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            32020..32640
                     /locus_tag="PKH42_00160"
     CDS             32020..32640
                     /locus_tag="PKH42_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA18866.1"
                     /translation="MIFEALEVGIYCANCYIVGSEETKEAAIIDPGADFNKIEGKLHE
                     LGLTPKIIILTHHHGDHIGAVKEFTDKYGLKVYIHKEDAEALNNSSMNFTKSMFGKNI
                     LIKADVELEDGDVIYLGELKFEIIHTPGHTKGGICIKVGNIMMTGDTLFNSSIGRTDL
                     PGGSYEEIISSIKEKIFKFDDDTVIYPGHMSPSTIKKEKKFNPFVK"
     gene            32660..34126
                     /gene="hemZ"
                     /locus_tag="PKH42_00165"
     CDS             32660..34126
                     /gene="hemZ"
                     /locus_tag="PKH42_00165"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOA18867.1"
                     /translation="MFKFYFFGHDYEYEARNALRVFDLNIDYEIMHGSFDGRSLSVLS
                     YLEEGEIISAKAQLYNDCELLYESCISENDIVLEKYSLKKLKKTLVIKTIHNVLKSYY
                     KVYPDYGILTGVRVVKILITAKKYSKSNEEINKILRDTYEVKEEKIKLLWDILNIEEK
                     YIDENINKKNYNLYIGIPFCPSKCSYCSFTSYVNCTDKKIFTYLDTLIYEIEKTIELA
                     KKKELNLHTIYIGGGTPSVLNEEQIETIFNAIKKYYDLSQVKEITFEAGRPDTINSEK
                     LICLKNNFVNRISINPQTMNDNTLLSIGRKHLGFEVVEKYLLAREIGFQTINMDIILG
                     LPGEDESDVKNTIEIIAGLNPENITVHSLAYKKHSELTRESAKLFKDYELIKKMHHVI
                     KEVCEVKCYKPYYMYRQKNIKGNSENIGYTKKDRECIYNIVMIEELETILGCGLGASS
                     KIMTGENKHETVRNFKSLEEYSKRIDEIINKKQTLLGG"
     gene            34127..34387
                     /locus_tag="PKH42_00170"
     CDS             34127..34387
                     /locus_tag="PKH42_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA18868.1"
                     /translation="MKKIKLFYIEGCPHCRKAFRILDELKAKNPEYSKIDVEYIDENK
                     DVVSANLYDYYYVPTFFVDDVKMHEGVPTEEKIERVLKEAIK"
     gene            complement(34432..34761)
                     /locus_tag="PKH42_00175"
     CDS             complement(34432..34761)
                     /locus_tag="PKH42_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOA18869.1"
                     /translation="MEHFMETAVHYVAYTLEIVSIIIVAYSAVTTLIKYLMSKFEFTN
                     KQAKIEFSRALEMALAYMLGAEILKSLIAHSKEQLITLAALVLLRAAIALIIHFETEA
                     DAKHCEK"
     gene            34987..35751
                     /gene="pdaA"
                     /locus_tag="PKH42_00180"
     CDS             34987..35751
                     /gene="pdaA"
                     /locus_tag="PKH42_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOA18870.1"
                     /translation="MIVVLLFACLLIYNTFKNFSVTVSTNISNWGLKFNENGTAPMGN
                     AGKDFLKKYNAYYVGEPDKKYIYLTFDAGFENGNTPNILDTLKKHDVKAAFFLVGHYL
                     EKEPGLVKRMVREGHIVGNHTYHHPDMSLISDKESFRRELTSLEELYKEITGEEMPKY
                     YRPPQGKFSEENLRMADELGYKTIFWSLAYVDWYKDDQPTKEQAFSKLIPRIHPGAVV
                     LLHSTSDTNEMILDELLTKWKDLGYTFKTLDDLTAN"
     gene            36104..37876
                     /gene="aspS"
                     /locus_tag="PKH42_00185"
     CDS             36104..37876
                     /gene="aspS"
                     /locus_tag="PKH42_00185"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOA18871.1"
                     /translation="METMGTLRRTNMCGELNKEHIGKEVVLMGWVQKRRSLGGLIFAD
                     LRDITGLIQIVFDADVNKEAFEKADSLRSEYVIAIKGKVFERQSKNLEMATGEIEVYA
                     QELRILDTAETPPIYIKDNDDVSEQMRLKYRYLDLRKPSMQKNLLMRSKTSKVIRDFL
                     YENGFNEIETPFLAKPTPEGARDYLVPSRISPGNFYALPQSPQIYKQLLMVSGMNRYF
                     QIVRCFRDEDLRANRQPEFTQVDIEMSFVDAEDVMAINERLIQRIFKEVKNIDIKLPL
                     RRMSYNEAMDKYGCDKPDLRFGYELKNITYVFKNSEFNAFKSVYENSGLIKCIKIDGA
                     SDDYSKKGISKLEKFVKSHGAKGLAWFKYENGLFDSPIAKFLSEGEINELISLLELKE
                     KDIVFIVADNESAVNTALSALRIEIAEERNLIDNNVYELVWITDFPLFEYSEEEERYV
                     AKHHPFTSPVDEDIDKLESAPEEVRAKAYDIVINGDEIGGGSIRITNRDLQNRMFKAL
                     GFTEEEAQKKFGYLLEAFKYGVPPHGGIAYGLDRFMMLLTGANSIKDVIAFPKTQSAS
                     CLMTESPSQVSEKQLDELKIEINE"
     gene            37899..38321
                     /locus_tag="PKH42_00190"
     CDS             37899..38321
                     /locus_tag="PKH42_00190"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOA18872.1"
                     /translation="MDNIGKIEKIDGNMAVISVKRVSSCGDSCKSCSSACKVNSVIIE
                     TDISDVKDEINVGDYVEIRAENEVMLKHIAVLYGLPLLLMLVTVFIFQMILKGPNKDI
                     ISAVASLFSLIISFYLLKAYDKSEMKKNVLKFTVGKKL"
     gene            38418..39722
                     /locus_tag="PKH42_00195"
     CDS             38418..39722
                     /locus_tag="PKH42_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOA18873.1"
                     /translation="MNIFEMQYQNSIKKEGPLAERMRPLNLSEFVGQEHIVGEGKILN
                     RAIEADKLTSAIFYGPPGTGKTTLAKIIANTTNNNFTTLNAVTSGIKDIKEKISYAVD
                     ELSIYNKRTILFIDEIHRFNKAQQDALLPYVENGTIILIGATTENPYFEVNSALISRS
                     LVFEFKRIDIEDIKNVVRKSLVDKRGYGNRKVEIDEDALAHIAEYANGDIRSALNALE
                     LGVETTEEIDGIIKINIDIASECIQRKKIDYDKDGDNHYDTISAFIKSMRGSDPDAAV
                     YWLAKMIKAGEDPKFIARRIIICASEDVGNADPRALEVAVNAFKAVEIVGFPEGRIIL
                     AQAAIYIACSPKSNASIIAIDKAMNAVENESHEVPAHLKDKHYKGAEKLNRGIEYKYP
                     HDYDKFYVKQQYMPETINTKFYEPKESGYESRIKKFLEYLRQ"
     gene            39734..40231
                     /locus_tag="PKH42_00200"
     CDS             39734..40231
                     /locus_tag="PKH42_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18874.1"
                     /translation="MYKIFGKITDPNIENIISSKLNFDNLNVDQVYDIYVDFYNTDLE
                     EGMLNADISYEIKKEHYLFIKKIRALFENNQIRVNEFYLMGTISDLPENEINISIMKS
                     QGDKKKNVVWPCKEIFLYENQKNKLDALLISNQISEEDYESNLEFLKDELNIYENDEE
                     HKYIN"
     gene            40311..40571
                     /locus_tag="PKH42_00205"
     CDS             40311..40571
                     /locus_tag="PKH42_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOA18875.1"
                     /translation="MEVYIITFLHGNYAVAAFNRLQGFGVKNIHLIQTPFSIKNECDL
                     CIKTNDRKTLDIILNECRGRYPVSNVYTQINKNGSFVYKLLP"
     gene            40715..41161
                     /locus_tag="PKH42_00210"
     CDS             40715..41161
                     /locus_tag="PKH42_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOA18876.1"
                     /translation="MILSTKGRYGLKAAFELSKNYGLGPVSLKKISEKYGISENYLEQ
                     LFAKLKKSGYIDTVRGPQGGYLLAKKPEEITVGMILRTLEGEIKTSDCLGKEVCSRES
                     ICATRIIFEKIEKSINDVIDNITLADMYEEQKNILTEGRNEQETLS"
     gene            41139..42326
                     /gene="nifS"
                     /locus_tag="PKH42_00215"
     CDS             41139..42326
                     /gene="nifS"
                     /locus_tag="PKH42_00215"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOA18877.1"
                     /translation="MSRKLYLDNAATTRVKKEVIDEMRQYFDILYGNPSSQLYELGRK
                     SKEAIEKARKTLADFLNAEEKEIYFTASGTESDNWALKGVAFANLNKGKNHIITTKIE
                     HHAILHTCEYLEKFGIETTYLDVDRYGLIDIEQLKESIRPETMLISVMFANNEIGTIQ
                     PIEEIGKIAKDHGILFHVDAVQALGGIRIDVNKLHIDLMSMSAHKIGGPKGIGGMYIR
                     KGTRIDNFIHGGGQERGRRAGTEGVQNIVGFGKAVEIAARDFDKHVERLTVLRNRLID
                     GIKNNIPDVVLNGHPTQRLPNNVNFSYKFVEGESILLLLDMEGISASSGSACTSGSLD
                     PSHVLLATGLDHGTAHGSIRFTLSEELTEEDIDYTVETMKKIITRLRAMSPIKNDEDL
                     HKN"
     gene            42342..42770
                     /gene="nifU"
                     /locus_tag="PKH42_00220"
     CDS             42342..42770
                     /gene="nifU"
                     /locus_tag="PKH42_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOA18878.1"
                     /translation="MYSEIVMDHFRNPRNVGHMEKPDGVGQVGNPKCGDIMKIYLKIK
                     DDIIEDVKFETFGCGSAIASSSIATELIKGKSIHEAVELTNKAVVEALGGLPPVKVHC
                     SVLAEQAVKSALYDYAKKANKVIKGLENYKPEDDEIHCQH"
     gene            42967..45594
                     /gene="alaS"
                     /locus_tag="PKH42_00225"
     CDS             42967..45594
                     /gene="alaS"
                     /locus_tag="PKH42_00225"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOA18879.1"
                     /translation="MDRFSINNIRKEYLNFFKSKGHLTEQSFSLIPKNDKSLLLIGAG
                     MAPLKKYFTGAEIPPAKRMATCQKCVRTGDVENVGLTARHLTFFEMLGNFSFGDYFKN
                     EAIVWAWELLTDVLKIDKDRLWVTVYLEDDEAENIWHTKVGIPMEKIVRLGKDDNFWE
                     LEVGPSGPCSEIYYDTGEDRGCGSTDCKPGCDCNRFIEIWNLVFTQFDKDEEGVYHPL
                     PNPNIDTGMGLERVACVLQDKPTVFDVEPLVFIVNKVSEICKIPYGQNLESDVAMKVI
                     CDHSRAATFMIGDGVIPSNEGRGYVLRRLIRRAIRKGKSLGINGEFLTKTAKVVIDNW
                     KEVYPELGEKEKYILKVLSLEEERFKITIDQGLELLMKEIQKLKETNKKELDGNVAFK
                     LYDTYGFPSELTEEILKENGFSLNKEQFNAEFEEHRQMAREARKTTGASGWKDNDFTL
                     DTDKTIFTGYNKLEDISEVIAIVVGSEQVNTINEGQSGIIVLDKTPFYAEGGGQTGDS
                     GEFTADEMLAKVTDTHKFNNGIYLHDTNLVKGSISVGDTITAKVNDDQRKNTAKNHTC
                     THILHKVLREILGDHVNQAGSYVSEDRLRFDYTHFEALDKKTLKEIEKRVNEAILSDH
                     TVKTDILSMEEAKKSGAVALFDEKYEEMVRVVSVGDFSKELCGGTHIDQTAKIGMFKI
                     ISEGSIASGIRRIEAITGRAAIDYMQELDNLIDELSISMKTTKDELLSKANLLKKEAK
                     EKDKEIQRLNNEILKSNINEILDKYEEINGIKLFAIKFKDKDANTLREIADKIRDKNE
                     SCAIILASDLGDKVLFVSAVTKDLAKGGIHAGNMVKEAAVIAGGGGGGRPDFAQAGGK
                     NPEKIDEAINAVRKQIASI"
     gene            45732..45995
                     /locus_tag="PKH42_00230"
     CDS             45732..45995
                     /locus_tag="PKH42_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOA18880.1"
                     /translation="MDSNVNNTAKFDYSMGSLNEAREIISQVYKSLKEKGYNPNNQLI
                     GYILSGDPTYITNHNNARSLIRKIERDELLEEILSVYLNVIDI"
     gene            46106..47053
                     /locus_tag="PKH42_00235"
     CDS             46106..47053
                     /locus_tag="PKH42_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA18881.1"
                     /translation="MKYINLFEKKNVSRLCYGTLSLSSLQNLDTVENKVRLLNYAYEK
                     GINFFDTAQLYDNYNILKEFIKHKDRDKLVIASKSYAYDKNTAEYSINKALKEMNTDY
                     LDIFMLHEQDNGNNFLGHYKAVERFMKYKEQGIIKYFGISTHRVEAVKDSLKFKEIEI
                     IFPLINYKGIGIQDGTFEDMTEALKEAKENNKFIMAMKIYGGGHLIKEAEKAFKYIDS
                     LNYVDSIAIGMSTVEEIDANISYLENNELNINIKNKIKNQNRTLVIEEWCAGCGKCVK
                     KCKQNALAIVDGKCAVDMEKCVLCSYCAGECAGFYIKIV"
     gene            47061..47474
                     /gene="ruvX"
                     /locus_tag="PKH42_00240"
     CDS             47061..47474
                     /gene="ruvX"
                     /locus_tag="PKH42_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOA18882.1"
                     /translation="MDRLMGLDVGDKTIGVAVSDLLMITAQGVTTIKRTNIKNDLNEL
                     KKLIDEYQVTKIVAGIPKRLDGTIGIQGEKVLEFLEKMKKHIDLPVEFEDERFTTTMS
                     ERMLIEADVKRKKRKEVIDKLAAVQILSSYMQRTK"
     gene            47489..47731
                     /locus_tag="PKH42_00245"
     CDS             47489..47731
                     /locus_tag="PKH42_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOA18883.1"
                     /translation="MDNIIELINDEGKLEKLVVEATFHIDDILYAVLHKINSDEGMVY
                     YVEEMEDGSIVLVKVEDEEEIKEVQEIYEGIADDLI"
     gene            47755..48219
                     /locus_tag="PKH42_00250"
     CDS             47755..48219
                     /locus_tag="PKH42_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOA18884.1"
                     /translation="MADYLRTLLDKNGYKTTVQRKILYEVLSDNKDKHLSTEEIYDLI
                     KNRYPNIGIATIYRTLLLFEDIGLVYKHNFDDGFSRYEILSQNSNEIHQHHHLLCKQC
                     GKIIEVKEDLMNTLEEIIEKQYNFEILNHVVKFTGICAQCRDKENKDGRKET"
     gene            48200..49876
                     /locus_tag="PKH42_00255"
     CDS             48200..49876
                     /locus_tag="PKH42_00255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOA18885.1"
                     /translation="MAEKKHKLKVIPLGGLGEIGKNITVIEYHNDIIVIDSGMAFPGD
                     EMLGIDIVIPDITYLIKNKEKVKGIILTHGHEDHIGSIPYFLKKLNIPIYGTKLTLGL
                     VENKLLEHKIEDASLNPINAGDTLKLGCFKITPIRVNHSIPDSVGFAIDTPIGTIVHT
                     GDFKIDYTPINEDVIDLGKFADIGRKGVLLAFSDSTNVERPGFTESEKTIGQKFMDIF
                     KNCDSRIIVASFASNIHRLQQVIDAAVVNKRKVAISGRSIINAIKVSLELGYLNAPQG
                     TIINVNELNSLKDDEIVILTTGSQGEPMSALTRMANNDHKKIQIKEGDLVIISANPIP
                     GNEITVSRVINMLYEKGAKVLYDALTQVHVSGHARRDELKLMLTLLKPKFFIPVHGEY
                     RHLVQHAKLANEIGIPINNTVIGSNGDVIELTSKSIVKNNTVTSGNILVDGLGVGDVG
                     NIVLRDRRLLSENGLIIVVLSTDRSTGKILAGPEIVSRGFIYVRENIDLIEESKTVVR
                     KALEKCEDTNVKEWNNIKMVIKDSLGSFIYEKIKRTPMILPIIVEVDLEV"
     assembly_gap    49942..50041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            50174..50875
                     /locus_tag="PKH42_00260"
     CDS             50174..50875
                     /locus_tag="PKH42_00260"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOA18886.1"
                     /translation="MINNETIEAERTRVCLTIGRIAPDFTAVTTEGPITLSQYRGKWV
                     LFFSHPGDFTPVCTSEFIAFAQLQPEFEKRNVQLLGISIDSNPAHLGWLYDIFQMTGI
                     VMPFPLIADRDASIAALYGMVSPDRIYEQSVRDVFIIDPNQRIRSILIYPATNGRNMY
                     ELLRLIDALQVSTTYGVSTPANWMPGDPVIVPVASTLDEAIERGMDPESLELNCLAWW
                     YCFTDLSELTPLPNP"
     gene            complement(51001..51243)
                     /locus_tag="PKH42_00265"
     CDS             complement(51001..51243)
                     /locus_tag="PKH42_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18887.1"
                     /translation="MDRLNKISTPDLSKVGNLDVIKLRRDGQYNLTSAINGRITPNLN
                     LTSKRSNQRMEDKSIVNNKPSQFRPILGVNFHNIKR"
     gene            51394..52410
                     /gene="mltG"
                     /locus_tag="PKH42_00270"
     CDS             51394..52410
                     /gene="mltG"
                     /locus_tag="PKH42_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOA18888.1"
                     /translation="MKKIIALLCVLSIIAAAYLYLNNYIEENLEAVDLGDDSPIVVEI
                     PAGSTTNDIAEILFENNLISNIRVFKYYAEKTGSDAKLKAGTYILSKSMNADELLESL
                     IKGGSSGNTYNITVIEGLTNEDMAQSLSEQTALNYDRFIELMESPEIFNQDFEFLKDS
                     SVTNLQGYLMPDTYNIYVDSTEEDIIRVLLKQFDKFYIDEIKPRIDKTNLTFEEVINL
                     ASIVEKEALLDEERDEVAKVFLNRLSINMKLQSCATVNYANGEWKERLTLEDIEIDSP
                     YNTYIVEGLPPTPINSPGRVSIIAVLEPADVDYLYFVAKGDGSHYFSNTYDEHIDAAE
                     EYLN"
     gene            52422..53066
                     /locus_tag="PKH42_00275"
     CDS             52422..53066
                     /locus_tag="PKH42_00275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOA18889.1"
                     /translation="MNNINSSYINEYINSLIIEDSEKLNKFRDYCEKRSLPIIHKEVG
                     QFIKLVINLLNAKSIIEIGTNVGYSSIFMSKVMKDEGKVVTLERSEKFYNEALKNITD
                     FGLEKKIEVYFGDAVDILDQVEGTFDMAFIDAAKSYYRVFFDKCLKMMKPGGIILSDN
                     VLYQGMIASDELVVRRKKTLVRNLRNYLEYISHDERFVTSVLPLGDGLAVTLIK"
     gene            53147..54370
                     /locus_tag="PKH42_00280"
     CDS             53147..54370
                     /locus_tag="PKH42_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOA18890.1"
                     /translation="MLPKLLAPAGNLDKLIMAVQYGADEVYFAGKSMGMRAGSKNFTK
                     DDLKQGVEYCHKYGRKANITVNIIPHNDDLKGIEGYLKYLHKIGTDALIVADPGVVEI
                     VKQTIPDMKIHLSTQANTTNYHTANFWYKQGVNRIVLARELTFEEIETIIKNTPEDLE
                     FETFVHGAMCISYSGRCLLSSFMTGRFSNKGDCAQSCRWKYNLVEEKRPGEYYPIEET
                     GEGTFIMNSKDLCMIEHLQKFKELGIKALKIEGRNKSEYYTALTVRSYRNALDELKYP
                     ESERNTGYWKEELEKTSHRDFSYGFFFGNPYKDGQLYTHSSYIRTYDVVGIIRSYDES
                     SKIATVEQRNKFSEGDILECFGPKGKHFELKAENLTDENNNKIDSAPHPQQTVKMYIE
                     KNVEPYFILRKKLEE"
     gene            complement(54441..55142)
                     /gene="sigK"
                     /locus_tag="PKH42_00285"
     CDS             complement(54441..55142)
                     /gene="sigK"
                     /locus_tag="PKH42_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOA18891.1"
                     /translation="MIQELLQILLPLSIFIFGYITNGSSFPHPLTKEEEACLIERANK
                     GDIEARNILIERNLRLVAHVIKKYKTVGIDQEDLISIGTIGLIKAVSTFDEKKGIRLA
                     TYAAKCIDNEVLMTIRSEKKLKKEVSLQGPLGTDKEGNNIYLIDIIKYEGEEIVGRLD
                     KEEKIKALDEILDKTLQKRERFIIEMRYGIRNKEHKTQREIAKLLGISRSYVSRIEKK
                     ALKKLHDALVNNVGN"
     gene            complement(55266..55949)
                     /locus_tag="PKH42_00290"
     CDS             complement(55266..55949)
                     /locus_tag="PKH42_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18892.1"
                     /translation="MNNYRNNMPHYDSLPHNYNPMGINEHKNSNRMHLANTMPRMPGM
                     PDDMLQRPFIPETEMPLEIDGDMPALPRFPQQGIPSLIPPWLTQPEIPMMPGMQPTPG
                     MPGMPQMPGMQPTPGMPGMPQMPGMQPTPGMPGMPQMPGMQPTPGMPGMPQMPGMPQM
                     PGMPNGMPQMPGMPPDDGNMYPMMPGMPNGMPPINCEQLRELMRRMNCPMDGGGGENG
                     TVPPMNEEM"
     gene            56189..56587
                     /locus_tag="PKH42_00295"
     CDS             56189..56587
                     /locus_tag="PKH42_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HOA18893.1"
                     /translation="MINILLVTHGEFGKELLKSSEMIIGEVENAKSISFNHGDSFETL
                     LKKVEENIKNISDDELIVFTDMYGGSPYNAVNRAMKNRNFYHITGINFPLFIDIAVAR
                     DTYSLEEIAEKIIKNGKKSIVFVNEKFLSD"
     gene            56596..57057
                     /locus_tag="PKH42_00300"
     CDS             56596..57057
                     /locus_tag="PKH42_00300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOA18894.1"
                     /translation="MKNVFVRIDDRLLHGQVVVSWIPYLRAKEVVIADDEYANDEFMS
                     ELIKSSGPEGVIVHVKTIDETAAYLSDDNQNKILVLLRSIEGIKKLIKKTKISSINIG
                     GVGAAQGRKRYYNSIHLSDDELNILNDIANDNIYVEIRILPKDKAIIIKGN"
     gene            57059..58495
                     /locus_tag="PKH42_00305"
     CDS             57059..58495
                     /locus_tag="PKH42_00305"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOA18895.1"
                     /translation="MTFSDYCLLSIFYFWASSTAFSVGIGYYTIYRPIMAGMAAGLIL
                     ADIKTGMLAGAVVNIIFIDFISTGGSLKGDQCLTAILAAAASIIFKLNPLEAAAAAFP
                     FGFLGILIWKYRLKINNIFVHIYEKKYKENKAPDISLYNGLFPQLLLFMMSLAVIITA
                     FYLMFLFNKLIHFNSISVLLYYIGIILVIFSVSNVLYKIKIKYNYAIFISVFLAALVL
                     DMRAYILFILIVLSLLYLSEKKVSIVRNSFNGKLKRGDLVYSWFIWMNYSHSCYSYER
                     LMGLAFAHSMKNIIRKLYADEHEIAEAIHNHTEFFNTEPNMGTPIHGYIISLEEERML
                     NNQSFEDISYIKKGMMGISAGLGDSFTQAILAPLFISMSVMLCLDGSFYLAFIPVIIL
                     SVFILFISYNGFMNGYFQGRESMLQRIKDVKQSKIKLYFPYIFSGILGFSISKLLFNN
                     IRPYEDIFTLSIILFAAVIMFIRKRGEQ"
     gene            58492..59109
                     /gene="nadD"
                     /locus_tag="PKH42_00310"
     CDS             58492..59109
                     /gene="nadD"
                     /locus_tag="PKH42_00310"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOA18896.1"
                     /translation="MKKKVGIMGGSFDPIHMGHLVAANEALNIYKLDEIIFIPTGDPP
                     HKDYPNAESFHRLLMVNLAVLTNDKFSVSDIEIKNPSKSYTLSTLRELHKLYKNTEFY
                     FITGTDAVLNLPSWHQPEEVLKLCRFIAATRPGISIEEVNTKINEIKRMFGGNIEILQ
                     VPMLQISSSDIRNRIKMGVSIKYLLPDSVEQYIIKNSLYKNGQEK"
     gene            59144..59644
                     /gene="yqeK"
                     /locus_tag="PKH42_00315"
     CDS             59144..59644
                     /gene="yqeK"
                     /locus_tag="PKH42_00315"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOA18897.1"
                     /translation="MHIEEMKKELKIELGEKLYRHSIGTMEEAEKLASVYGCDVSKAI
                     IAGLLHDCGKRICSDNLKHAKKSAELAGVKYGVKDNDILNAIMFHTTGRVNMSLLEKI
                     IYIADKIEPNRNYEGVEEIRNKAYTRLDEAIIKSLENTIEYVKNRNLVLDMESVNTLN
                     YLKEGK"
     gene            59641..60009
                     /gene="rsfS"
                     /locus_tag="PKH42_00320"
     CDS             59641..60009
                     /gene="rsfS"
                     /locus_tag="PKH42_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOA18898.1"
                     /translation="MIEVQKNIETILKACDNKKAYNFVVLNVSKTSSITDYFIICSGN
                     NEKQTVAIAEEVLEKGAKEGIDFFYKEGFETGRWILLETDDIIVHIFHKDERAIYDLE
                     SLWYDNNSLDVEQYGIKNWK"
     gene            60016..60399
                     /locus_tag="PKH42_00325"
     CDS             60016..60399
                     /locus_tag="PKH42_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOA18899.1"
                     /translation="MMNSVIHTNNAPAAVGPYSQGIKAGNTIYVSGQLAINPATGELY
                     KGGDIKEETRMSLMNVKAILEEAGATLKDVVKCTVFIKDMNQFGLINEAYAEVFQDTK
                     PARVCVEVARLPKDGNVEIDAIAVV"
     gene            complement(60660..>61651)
                     /locus_tag="PKH42_00330"
     CDS             complement(60660..>61651)
                     /locus_tag="PKH42_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOA18900.1"
                     /translation="LTLKDLLYALMLPSANDAASVIAKHYGGSLENFVKMMNQKAKEL
                     GAYNTNFTNPHGLHDTNHYSTAADLALITKYTMENDTFRKIVSTTKYEIQTTNKKDEP
                     RYFTTLNKLIYNTSYNQIYVDGAYISPYYEYATGAKTGYTPQAGNCLVATAKKDGTEL
                     IAVTMKGISLEMYQDAHNLFNYGFEEYESATLVGKNTFIKNIKIPSGDSKEISLITES
                     DLTALIKKDTLDDIKSNVYINDITLPIEKNSVIGKIEYTLDNKVIGAVNLISPISVKS
                     TAQEGQGNILLSIVKFIGFLILFAAVSILLLKIYNDIRIRINRKKRRSRYNNY"
BASE COUNT        22100 a         9030 c        12306 g        17915 t
ORIGIN      
        1 aaatgttgga ggtaataatg gattcaaaat tagcgttata tattgttaag agactgacct
       61 tggcagtgat aacaatattt ttaataatca ccattacttt tttcgtaatg catgaagttc
      121 cgggaggtcc tttcttgagc gagaaagcac caagtgcggc tgttacggcg gcgctggaag
      181 caaaatacgg attggacaaa cctaagtctg aacagtatgt aacatactta aagggtatta
      241 tgaaaggtga cttcggtcct tcgattaaac aaagaggaag agaagtaaca gcagttatta
      301 tgcagggctt taaagtaagt tttaaattag gaggaactgc tgcggtgata gctctttgta
      361 taggaactgt attaggctca gttgcggcta tctttcataa taagactata gataaaataa
      421 ttatggttgt tacaaccggc ctggtcgcca tgccgtcatt tattatagca acattgcttt
      481 tactggtctt ttgtgtgtgg atgggaattt ttccggccaa tgggaatgag cccggcggac
      541 taatacttcc tattatatct ctcagccttt atcccatgtc atacactacc agacttacca
      601 ggtccagtat gcttgatgta ttaagtcagg attatataag aacggcaagg gcaaaaggag
      661 tgaaacctgc aaaggtaata tttaaacacg ctctccgaaa tgccgtaaca cctgttgtaa
      721 catatgccgg tccaatgttt gcatatatta ttacgggaag tttggttgtc gaaaaaatat
      781 ttgcagtacc gggtcttgga aaagaatttg tttcaagcat tataaatcgt gactacacta
      841 tgattatggg gaccacaata tttttaggta ctcttataat tataatgatg cttgtttcgg
      901 atattttgta caaagtattt gacccgagaa tagaattggg atagaaagag aggatgtgac
      961 atatgaataa tatagatgaa atgcctaaaa agaatcctct tagttttcaa cttaatttaa
     1021 aggattttga aaaagcaaca gaagatgaaa aagctcagca ggtaacgatg agcgaaagta
     1081 tcaccttctt taaggatggg atgagaaggc taagaaagaa caaaatagca atgacatgct
     1141 tgactgtatt gattctgata gctttaatag tgactttcgt acctatggtg tatccctata
     1201 cctatgagca gcaattagga gtggcacaag gaaaaagaat cgataaaact tataataatt
     1261 taaaaccctt tgaatatgga gagacagaat tggagcgtat tgcaaacggt gaaaaaatat
     1321 ttcctcatat attcggaact gatgctgcag gaagagatta tgcaataaga gttatatacg
     1381 gcggaagaat ttccttgctg gttggatttt tcgcagcaat aatcgtattg ataataggag
     1441 tggtttacgg ctctattgca ggttacttcg gaggaaaagt agaccttttc atgatgagga
     1501 ttgttgatat tatttattct ttgccggaca tgcttattat cattttgtta tcggttgttt
     1561 taaatcaagt attgcagcct aagatagaag gtacttcact tgcaaaagtg ggtacggata
     1621 tgataagtat atttatagta tttgctcttc tgtattgggt tagcatggca agacttgtgc
     1681 gcggtcaaat tttatcaatt aagcaaaatg aatatatatt agcagcaaaa tcagttggct
     1741 ccagtccatg gagaattata aagaagcatt tgcttccaaa cagtatgagt gttataatta
     1801 tttctacagc attgcagatt ccgtcagcta tatttacgga aagctacttg agttttctcg
     1861 gtttgggtgt ttcaataccc atgcccagtc taggttcatt agcttcggat gcattgaccg
     1921 ggatttactc ttatccatac agattaataa tacccgcaat agctatttgg ttgatagtat
     1981 tgtcctttaa cctgttgggt gacgggctta gagatgcatt tgacccaaaa ttaaagaagt
     2041 aggaggaatg aaatgagttt attacaagta aaagatttgt atacttcatt tttcacttct
     2101 tccggcgagg tgaaggctgt taacggagta tcatttaact tagaagaagg caaagtgctg
     2161 ggtattgttg gagaatcagg ctctgggaag agcgttacag catattcaat acttcaaatt
     2221 ttaacaaatc ccggaagaat tgtaagcggc agcataaaat taaaaggaca ggaattggtt
     2281 ggctgcagca aagaaactat gcagaaaata agagggaacc aaataagtat tattttccag
     2341 gaccctatga caagcttgaa tcctgtattt actataggaa atcagttaat ggaagcaatt
     2401 ttattgcata caaacagaaa taaagaagaa gctaaagcta gagcaattga aatgcttaaa
     2461 ttggtcggtg taaatgaacc ggaaaaaaga atagagcaat atcctcacga attttcggga
     2521 ggtatgagac aaagagttat gattgcaatg gctcttgcct gtgaacctga tattttgata
     2581 gcagatgagc ctacaacggc tttagatgtt accattcagg cacaaatact tgatttgatg
     2641 aaagagctgc aaaagaaatt aggtatggca atcatcctga ttacccatga cttaggtgta
     2701 gtggcagaaa tgtgtgatga tattatcgta atgtatgccg gacaaatatc ggaaagagga
     2761 acagcagacg atatttttta caatccaaag catgaatata ctaagggatt gttaaaatcc
     2821 atacctacgg tacaagatga aaaacagcgc cttaagccaa tcggcggcaa tccggtagat
     2881 ttattaaaca tgccggcagg atgtccattt gcaccaagat gtgacaatgc aatgaaaatt
     2941 tgtcttactg taaatcccgt tgagatgacg atagaaggca aacatacttc caggtgctgg
     3001 atgaatgtaa gagaaggaat tgaaaatgaa actcttacaa taaaacaagt agaggagatg
     3061 tatcatggaa aataacaaca atcttcttga agttagaaat ttaaaacagt atttcgatat
     3121 tccggtcggc ttgttaaaga gcaaaccttt aaaagcagtt gatgatgtct cattcagtat
     3181 tagaaaaggc gaaaccttag gtttggttgg tgaatccggt tgcggcaaaa caacagtggg
     3241 aaggtctata ttgcagttgt acaaaccaac tggaggagaa ataattttta aaggcaaaaa
     3301 gattgaaagt aaagagtcta taaaacaatt aagacaaaat gcacaaatgg tatttcagga
     3361 tccatattct tcactaaacc cgagaatgac agtcggggat attgtgggag agcccttgga
     3421 tgttatgaat atgtataaga gcagaaaaga aagagacaaa agagtaaatg aattaatgga
     3481 aattgtagga ctgtcaagcg agcatgctac tcgttatgct catgaatttt ctggagggca
     3541 gcgtcaaaga ataggtattg caagagcctt ggctgttgag cctgaattta tagtgtgtga
     3601 tgagcccgta tctgctctgg atgtttcaat tcaagcgcaa attatcaata aatttgaaga
     3661 tttacaagaa caattgggac tgacttatct ctttatcgct catgatttat tggttgtaaa
     3721 acatatatcc aatagaattg cagttatgta tttaggtaga ataatggaat tagcgccttc
     3781 cgatgaattg tataatcacc cgcttcatcc atatacaatt tcgttaatgt cggcagtacc
     3841 tattcctgac ccgagctatg caagaagcca tcagaggatt ttacttgaag gtgatgtacc
     3901 aagtcctctg aatatgcctt ccggatgtcc ttttagaaca agatgtaaaa gggcaacaaa
     3961 agaatgcgct gaaatcaggc ctgaattaag agaagtggca cctgaacatt tagttgcttg
     4021 tattcatgtt tagaggttta gggacacacc ttcaaagtat ggtgactcta taaagaatag
     4081 ctttgaagga acgtccctga ttagagggga cacacctata tgtagtgata gtctttgaag
     4141 ataagcatag aggaatgtcc ctaattcgag gggaacatac aaatgtctct gacttgtgtg
     4201 atattaattt aaaaattaat atatatttat ttaaaggagg aaagtaatga aaaaaatttt
     4261 atctttaatc ttagtattgg ccatggtgtt ttcattagca gcatgcggaa ataatcaacc
     4321 accggcagaa ccggaaacac cagaaacccc tgaaactccg gaaactccgg aagaaccagg
     4381 tattgcagat agtttgactt tgcatgttgg tgcatatcct gatactattg acccggcact
     4441 taacagtgca gttgacggtg ctacttatat aatccacgca tttgcaggat tagtaggcta
     4501 tcaacaaaaa gaagacggat ctcttgaact ttatcctgat ttagctaaag aattacctgt
     4561 gggcaaagag agagacgacg gaaaggttga atatgtatac gagttaaaag acggcttgaa
     4621 atggagcgac ggctcagact taacggctca tgacttcgtt tatgcttgga acagagcagc
     4681 taacccggaa acagcagcag actatggata tatgtttgaa gttgtagacg gttatgcaga
     4741 aatgacagaa atggatgatg aaggaaatct tgtaaatcct gatgcaaagc ttaatgttac
     4801 agcatctgaa gatggaaaaa cattgactgt tgttcttccc gtagatgtgc cctacttcaa
     4861 tgaattagtt gcattcccga catatatgcc tgtaaaagca gaagttgccg aaggaaatga
     4921 tgcttgggct acagcagctg atacatatat tgggaacgga ccatacaagg ttgttgaatt
     4981 ctcacaaagc cagttgataa tgcagaagaa cgaatattat catgatgcag accgcgtaat
     5041 tacaaatgaa ttagtatttg catttaatga tgatgatact tcaactttag caaacttcca
     5101 gaacggctca tacttgttca tcgaccaagt tccaaatgat gaaattaaat cattgcagga
     5161 gcagtatcct gatgaattct ttatagaagg acaacttgga acatactatg tttctttcaa
     5221 cgtaaatgat gagaatttca aagctttcgg tgaagctgac aaagcgaaaa taagaaaagc
     5281 tcttggtctt atgattgaca gaacttatat agttgaagaa atcggccagg caggtcaggt
     5341 accggcagca ggttttgtac ctatgggatt aacagagccg gatggaaaaa cagaatatat
     5401 agctaaaaat ggtcctaaag gagacggagc aggatattac agcgttgacc cggatgatta
     5461 tgaaaagaat tgcgaagaag ctgtagcttt gttaagagaa gttgcagaat caacaggatt
     5521 gtacactgtg gatgaaaatg gcgtagtttc aaaagaattc ccgacaattg cttatttaac
     5581 aaatgaagga acaggccatg aagcaattgc cataaatctg caagctactt ttgcacaata
     5641 tggaattaac atgacaattg aaaaacaaga atgggcaacc ttccttaaca ctcgtaaaaa
     5701 tggggactat tcagttgcaa gaaacggctg gttaggagac tacaacgatc ctatatcatt
     5761 ccttgacatg tggattacat caagcggtaa caacgattca caatttggca agggcgagca
     5821 tgcaacatat gcaggttaca cttacaaggg agaaggcggc aaaacttggg cagaatcata
     5881 tgataagata attgctgaag taaaagcttc taaagattct gaagaaagat ttgctttaat
     5941 gcatgaagca gaaaatatat tgatggaaac aggagctatt tgcccaatct actactatac
     6001 agatatttac atgaagagta cagatcttca aggtgctttt gcaagcccat taggatataa
     6061 atactttatg tattcttatg tagaagaata acataatata ataagcatta actaagccgg
     6121 gagaaatccc ggcttttgta atcctatgaa taacattatt gtcattctga gagctttagc
     6181 tcgaagaatc ttatgagatc tttgctacgc tcgggatgat gaacagtatc attatgctgt
     6241 aatcattctg tttatttttt ttgttttctg tagtataatt aaaaatagta cagagcagga
     6301 gactaaaatg aaatacaaaa tggtagtatt ggatttagac ggtactcttc taaatagcga
     6361 aaaaacaata agtgataaaa atgcacatat acttatcgaa cttcataaaa ggggcataca
     6421 tattgttata gcaacaggca gaaattatta tatggctaaa actttaatag aaaggcttaa
     6481 atttacggag ccggttattt tagcaaataa tggtgcggtt gtaaggcatt atgtgaatga
     6541 cgaatttatt gaaagcaatt atttggattt ttctacattt gagagaatat atgaattagg
     6601 tttaaagcat gatttaaatc cgataattca tgttgatgaa tacaatcaag gatatgacat
     6661 tatttacgaa tgtgaaaatt acgaagaagt atacttagga tatattaaga aggattataa
     6721 aagaacaagg tgcaaaaagt ttaagacaga ggaaataaat aatattttgt cggtttgcta
     6781 cttcaatgaa tatcaaaaat tattaaaact taaaagtgaa attgaaaaaa taccaggtaa
     6841 gttcaatata atactcaatc aaaacataag caactggtct ctattggaac tgctaaatat
     6901 taatggatgt aaatggtgtg cattaaaaaa atatatcgac agactgggaa tagatgcaaa
     6961 tcaagtaatt gccatcggag acgacaataa tgatattgag cttattaaaa actcaggtct
     7021 gggaatagct atgaaaaatg caaccgacga attaaagaag gctgcagatt ctatttcgcg
     7081 cttttcaaac gatgaatccg gagtgtatta tgaattgtca gaaatattca aaattaaata
     7141 aaaatattta agctttgtac atattttcat aactcctaaa atataatgta ttaatattat
     7201 gcaatattga ggaggaacta tgagaataat tactgcaata gcttcaattc ttataattat
     7261 aggagcttta aactgggggt tgtacgggct atcaaaaatt gatatagtgg aaaatttgtt
     7321 tgggggctgg aaaagtaaat ttggcaggat tatttatcta ttaattggtg cagccggctt
     7381 atattcatta ttgtatgtga tttttgcttg aataagggca gacaaaaaac aaaaacatgc
     7441 tcacgcggca tgttttgttt ttttgggcat ctgagatcct tcgcttgtgc tcaggataag
     7501 cgactgccgc caaatcacag atttggggca tctgagatcc ttcgcttgtg ctcaggataa
     7561 gcgactgtca ccaaatcaaa gatttgggac acctgcttac ttgaagggtg ccatttttgt
     7621 cattctgttc attatgcggc tgtgctgcca attgcttgaa actaattcag ctgaacctat
     7681 agcaatattc gctaatccaa atccgattaa tccgtttctg taagattccg gcaggtttgt
     7741 gttttttaaa gcaactcctg ttgcagtaac aactgtaccc aaaatagtcg ggacgatacc
     7801 ttcttttata cgcatatgaa gcctccttta tttaaatttg tattaatatt atttacattt
     7861 aatcgtaaaa taattttatt aattactggt aatttaattg atttaatcta ttaacttaat
     7921 tataatgatt aaagaaaaat tattataaaa agggaaaaat atgaaattgt ataataagta
     7981 ttctgattat ttaaaactaa agtatggcga aaaagtatat aaacttcccg taaatattcc
     8041 atgtacctgt cctaacagag acggaactct gggttacgga ggttgtacat tttgtgccga
     8101 cgtgggaacc ggttttgaaa tgcttgataa ttctttaagc gtaaaagaac agttaaataa
     8161 aaatatggac tatatttcaa aaaaatataa gagtgggaaa tttattgcct actttcaaaa
     8221 ttatacgaat acatatatgg atatagaaag gtttaagaat tatataaatg atgcaataat
     8281 ggataatata gtggaaatag ccatatccac cagacctgac tgcatatctg atgaaatact
     8341 tgagttcctt aacgaaataa aaaaacaaaa tgtaaatata acaatcgaat taggacttca
     8401 gacagtaaac tatcatactt tgaaaaatat taacagaggt cataccttag cggaatttat
     8461 tgatgccgta ttaaggatta agaggtataa ctttgaaata tgtacacata ttatattgaa
     8521 tttacccgga gataccattt tggacacggt tgaaacggca aaaatattat ctgttttaaa
     8581 aattaaccag gttaaaattc attctcttta tataatggaa aataccgaaa tgggaagagc
     8641 atataaaaac aatgaaataa cactcatttc aaaggatgaa tatgtagaaa gagtaattgt
     8701 ttttctggag catttagatg tggatattac agtacaaaga ttgataggaa gggcacccga
     8761 gaaagattct ctttttgtaa actggggtat gagctggtgg aaaataaatg atgatattct
     8821 ttataaaatg aagtctgaaa acaggtatca aggcagtaaa aataaatttg tttaattgta
     8881 aataaaatgg tgtaaacaat tacaagatgt ggtacaatat aagtgtcttt actattataa
     8941 aattatgaat aggagtgtga ttattatggt tcaattggtt tgcggtcata gcggcaccgg
     9001 taagacaaga aggatgattg aaatggcaaa caacgctatt gacaaaattc acggcagtat
     9061 ggtttttttg gaagcaagca acagacacat attcgattta gattacagga taaggtatat
     9121 taacacaaaa gaattcggat taaccaatga tgaagagttt caaggttttg tatgtggagt
     9181 aatcgctact gattacgaca tagagcaagt ttacatagac ggattataca aaatagctaa
     9241 gtcaggcaag gaaagattgg taccattaat agaaagacta gattacttat caaaaacatt
     9301 tgatgttaat tttattataa gtgtaaacca tgatattgaa gatattccag aaggattgag
     9361 agatagagtt atagattaat ttatgccgcc ggtttttgac cggcggttat tatatccggg
     9421 gatacctctt ctttaaagca aattaaagca tatcttgtaa gaaacgtctt ctctaagaac
     9481 catatatatg gtatatagca cttgctaatg taacattttt ctgttagaat aggcattgta
     9541 ctaaattaga aagagtgatt acatgaatta ttttaaatcg aaaaattcta tattcgatga
     9601 tttttttgag catagggata tgctcataat tcagcacatg aatggagata taaataagaa
     9661 agaatacttg gagcttaatt ataaatacat gcttgaaaaa aacattaaac catttcaacg
     9721 tattgacagc tttgaaaaag gaatgtacaa ctatcaatat tacaatatga tggcaaaata
     9781 ccaccgaatg atagcacagg aaattaagga taagggaaag cacatcagtt actataataa
     9841 atatataaaa gaagctgatt tttattacaa tgaaaaggat aagacaattt tccgcctgtt
     9901 aaggtttctt caatttgaaa acatagaatc atattttatt aaaatggaat catcattatt
     9961 agaaggcaag ctttacgaaa tagtattgaa ggattttgaa tatgctgtac ttcattctaa
    10021 gagcttgtgg cttttggatg ttttaaaaaa agaaaaagta tttacagaaa agaggaagaa
    10081 atcagttatt gattattatg taaatcaacg gtattaaggt gtggaaacac accttctttt
    10141 attgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatat ttaataagca
    10261 ttcgcaatta ttttgataac atcatcaaga ggtgttccta cttcaatatt aaatgttccg
    10321 ctctgcagtc ttgtatcaag gtcaagctca acacagcgag taagaaactc atctttgctt
    10381 tttattaaat tgttgtttag cagaatttcc gctatttgtg aagggaatga gccttgagga
    10441 atactaatgg cagcaattaa ccctgtcggt gtttcatctt ccggctccgg ctcttcgtct
    10501 tccgggtccg gttcttccgc aatcggttca tttacttcag gttcatttat tataggcagg
    10561 tctttaatta tttctttttc aattcctctg ctgaacaaca aatcaaatct ccaaaccagt
    10621 atagctgtca taacaacaat tacggttata ataagtccgt aatcagttat attatatata
    10681 aagtctctta ttgcgttaat tattcttttc atttgtcacc tcttagctta tcgctcttat
    10741 tatattaaca caaaaatgag atgcatacaa tatccacatt taataaataa ttgtaaaaac
    10801 tgtaaaatac atatactttc ataaaatatt tgttataata gtatagagaa acatagtgct
    10861 cgtcccctcc ggacaaatat ttaatttacc taagaaaaag taaaggataa cacatgaaag
    10921 aaattaaaat taccaaaaat gaagaaggtc aaagactgga caggttccta aagaaatatt
    10981 taaataaggc aaatcagtca tttatacata aaatgattag gaaaaagaat ataaaggtaa
    11041 acggcttaaa agcaggaccg gatattgttt taaaaacaga tgacacagtc cagctgtatt
    11101 tgtctgatga aacaatagat aaatttagag ataagaaaac acttcaaaag accgatatac
    11161 attttgatat tgtatacgag gatgataata tattagtagt aaacaaaccc ataggcctaa
    11221 gcactcagcc tgataaaaca ggaaagaaaa atttagttga tgagataaaa gtatatttag
    11281 atgcaaaaga agaaaatatc agttttacat ttaagcctgc cgtgtgcaac cgcttggata
    11341 aaaatacatc aggccttgta atagcagcaa aaaactatga tgccctgaaa cagacaaaca
    11401 aggcaataag agaaagaaaa atcagaaaat actacatggc aaaggtacac ggaatcatta
    11461 aggatgattt ggagcttaag gattatctta ttaaaaaaga caataaaaat atggttaaaa
    11521 ttataaatga gtacagagaa aattcaaaat cagtcataac ctatgcacat cccttgaaaa
    11581 gagaaggaaa atatacatgg ctggaagtag aaattgaaac aggcagagca catcaaattc
    11641 gggctcattt agcttcaatc ggacatccca tagcaggaga caaaaaatac gggaaaaaag
    11701 acaatgaaaa atatcaggta ctgcatgcgt ataagttgtt gcttgatgga tttgaaggca
    11761 atttatcata tttaaacgga atggaaattg tatcggatat cagtgaaaga aaaatattgt
    11821 aggaggttag gaatgaaaat taaattagca aatactgacg aagtaattga agtatacgaa
    11881 aatgaaacat tataccggat acttaagaga gtttataaaa atgattattt taaatatttg
    11941 ggtgctaagg taaacaataa gcttaaggga ctttgcagca aagattttaa ggaaaacgac
    12001 aggatagaat ttattgatat tacaaatccc gatggtcaca gaatttacgt tcgcaccttg
    12061 tcattaatct atgttaaagc atgtaaggac atttataacg atatagatgt ttccattaat
    12121 cattctttaa ataaaggact ttatacggag cttcaatata aacatttagt aacaaaaaaa
    12181 cacctcgatg ccataaaaaa taaaatgcag gaaattattg atgccaaaaa aaatattaca
    12241 acttactttt taagtgttga caaggcaagg gaaatatttt cggctcaggg tatgatggat
    12301 aaggttgaaa tgcttaagtt ttggaataag gatacaataa gagtgtatga attggacgga
    12361 tattatgata ccttttacgg atatgtcgct ccaaatacag gatttgtaaa taagtttgat
    12421 ttaagattat tttatcccgg aataatacta aattatccca cccgtgaata caattttgaa
    12481 cttcccgaat atattgagca aaagaaactt tcaaaagtat ttaaggaagc tgaggactgg
    12541 ggggaaatat tggatgttgc atatgttggc gctttgaaca aaaaaatcgt caataatgaa
    12601 atatatgata tgataaggat taacgaagct cttcacgaaa aaaagattgc atatattgcc
    12661 gatgaaatta caaatgataa aaatataaaa atagtcctaa ttgcaggtcc ttcttcttca
    12721 ggtaaaacaa cattggcaca aagattgtcg attcaattac gagtgaacgg caagaaaaca
    12781 tatgcagtat ccttggatga ttattttgtt gacaggcatc tgacaccaaa aaatgaaaaa
    12841 ggagaatatg attttgaaac tatcaaagca ttagacctgg atctttttaa tgagcagctt
    12901 atagatttaa tggcaggaga agaagttcaa atacctgtat ataattttaa ggaaggtagc
    12961 agggagtata caagagaacc tgtaaggtta acaaatgacc atattataat aattgaaggt
    13021 atccacgggc ttaatgatga acttacagct aatataccgc aagttaataa gtttaaaatt
    13081 tatataagtg cactgacaca gctaaacatc gatagacata atcgtatttc gacatctgat
    13141 ttgagactat taagacgtat tgtacgagat tacacccaca ggggaaacaa cgcaattaaa
    13201 acaatggaac tgtgggataa tgtgattaaa ggtacagaaa aatacatttt cccttaccaa
    13261 gaaaatgcgg atgcaatatt taactcggca ttagtttatg agttgtgtgt tcttaaaaaa
    13321 tatgcagagc ccattattat ggaaattgat gaagacagct gtttttattc agaaagacaa
    13381 agattgttga agtttttaag ctacttcctt cctattgaag atgaaagcgc tattcctaat
    13441 acttcaatac taagggagtt tattggagaa agctgcttta gataatgata aggtgctagg
    13501 caccattggt ggtaccattg gtggtgcttg gcaccttata tacacgttta aaattcattt
    13561 aaatcgattt ttcctctttt cattcctaca gacaggcata gacctatgga tatcatgttt
    13621 acaagcatga aggtacctcc atagcttata aatggaagag gaattcccgt tacaggcgtt
    13681 agccccattg tcattcctat attttcaaat atatggaaca actgcattgc ggtaatgcct
    13741 gttactatta atgagccaaa catattagca gcatttttgg caattctgat aagtctgtaa
    13801 aataagacgg catacataaa caaaagaact aagcccccaa ctaatcctaa ttcttcgcct
    13861 attaccgcaa aaataaaatc tgtttccttg gttggaagat atcctaattg gttttgaaca
    13921 cctttgtaca atcctcttcc gtacagcatt cccgaaccga ttgccgtttt agactgtatt
    13981 acctgataac ccgtatcatc cgggtctaat tccggataga agaaggttac aattctgtca
    14041 aggcggtaat cttcatttaa ggtgtaatct tccattatct gaaagaatat actgagtccc
    14101 gcaataatta atattaaacc gattatcata acagaaagca catattttct gtcaatacct
    14161 gaaatataaa tcattattgc cacaaaaaat atgaatacaa cggcagttcc taaatccggt
    14221 tgcattaaaa ttaatgctat cggtaaaaaa gcgaacaaaa taatttttaa taacactacg
    14281 ggctggttaa tattatcttt gtggatatca ataaactttg acagagaaat tattattcca
    14341 aacttagcta tttcagaagg ctgaaacact atgggaccta ttgctatcca tgaccttaca
    14401 tctccccatt cggaagcttc aacgggattt atcaatgtgt acaaaagaag tatattggtc
    14461 atcacatata tcggaatata aaggcttccg taaatatcat aatcaataaa aatcaacaca
    14521 aataaagcaa ccataccaag tccgaaagct attatctgcg tttttaaaaa tggctcgcta
    14581 cctgttgctt tgctcattgt ggcactgttt attgctataa atccatatat acataaaagt
    14641 attgttgtaa ttaacaatat aaaatcaaac cttttaagtt tactttcttt tttaaacata
    14701 tttactccat gtcttccttg cttatactca ggatgacgta attttattag attaccccaa
    14761 ttatatcaca tcttaaaaca atggaaaaca aatatttcat gaattaagtt ctttatatta
    14821 gaacaaatgt atgctataat tttatataat aattgagggg acgaaatatg ctgatacttg
    14881 gtatagatcc ggggctggca atatcaggct acggaatatt aaattattca ggaaataaat
    14941 ttaaagtaat agaatatggt tgcatattta ccgaaagctg tgaagaattc ccgaagaggc
    15001 tcaaacatat ttatgaaagt tatatatcta ttatagatat gtacaagcct gaagccgttg
    15061 caattgagga attgttctat aataaaaatg caaaaaccgc aatggctatt gctcaggcaa
    15121 ggggagtgca tcttttggct gcagaaaaca aaaatgtccc tctttatgaa tatacaccac
    15181 ttcaaataaa gcagggaata gtcggttatg ggagagctga aaaaaaacag gtacaggaaa
    15241 tggtaagagt aattcttaaa ttaaatgaaa ttccaaagcc tgatgatgcg gccgacggat
    15301 tagcggcagc aatttgtcat gcacattcgc ttaaatttgc aaaagaattt aaaattaata
    15361 aatattgagg gaagatatga ttagctacat aaaaggtgaa gtagtaaaaa aaggcatcga
    15421 ctacttgata ttagataaca ataatatagg ttattatata aatacttctt tttcgacctt
    15481 agaaaaaata agtgagaaag agaaaattat tatacttaca tacatgcaca taagagaaga
    15541 tattattgct ttatatggat ttcttacttc tgacgagata gatttgttta aaaaactaat
    15601 cagtgttaac ggaataggac ctagagcagg actttccgta ctgtcaacat atgaggtaaa
    15661 tacagtaaaa gaaattattc taaaagaaga tgtatccgga ctgtccaagg ttccggggat
    15721 aggcaaaaaa acagcgagca aaataatttt ggagcttaag gataaggttg gaacagttga
    15781 agaactcaaa ggaagcgaga ttgttgtaag cagcgaaatg tccgatgtga tcgaagcctt
    15841 aacatctttg ggatttaact atgccgaggt caaaagaaca ttggggaata tggagctgtc
    15901 aggcaagtct gaaaatgata taattaaaga agcactaaaa aatatgaaca ggcagggata
    15961 atatgtttga aagagaaaat gaaatagttg caagaacatt taattcttgt gaagaggaat
    16021 tggagcttag cttaagacct cagaaactcc ctcaatttat agggcagaac aaagtaaaag
    16081 aagttttgaa catatttatt gaatctgcca aaattagaaa tcagcctctt gaccatgtat
    16141 tgctttcagg acctcccggg ttagggaaga ccacattagc caatatcata tcaaatgaat
    16201 taggagttaa tataagggta acttcaggac ctgcaataga aagagccggt gatttagctg
    16261 caatacttac aaatctgaat gaaaatgatg tattgtttat tgatgaaatt cacaggctta
    16321 ataagactgt ggaagaaatc atgtatcctg cacttgaaga ttttgccttg gatattataa
    16381 taggaaaagg tccttcggca agatcaataa gacttgattt gcctaaattc acccttatcg
    16441 gggcaactac ccgttccggt atgcttgcat ccccactaag ggacaggttt ggggttatgt
    16501 gcaatttgga tttctatgag ccggaagatt taaaagaaat agtattgcgc tcagcaagta
    16561 ttatgaatat tgaagttgaa gaaaagggag cagaagaaat tgcaaaacgc tcaagaggaa
    16621 ctcctcgaat agccaacagg ctgttaaaaa gggtcagaga ttatgcaatt gtgaagggcg
    16681 aagggataat agacttaaaa accgctgatg aagctcttaa aatgcttgaa attgatgaaa
    16741 taggtcttga taaattagac agacgaattt taaacactat aattgacttt tacaagggag
    16801 gtcctgtcgg aattgataca ttggcagctt ccatcggaga ggaaaaagga acaatagaag
    16861 atgtatatga gccttatctt ttgcagatag gatatttaaa ccgtacaccc agaggaagag
    16921 ttgtatccga gtcgggttac aggcacgtta aaaaaataag gaaaatacaa gatcaaatta
    16981 atatattaga ggacgattat gaagaagaat ttgattagac tatttataat aactgcttta
    17041 cttacttttt tatttccaac aaccgctttt ggtaaaaata aagatgacat atatgtaagg
    17101 gtaagaataa gatacccgag actttttaac gagcaggtat cttttgaagg atttgatgaa
    17161 atcgtattgt atgatgatga agatgagctg ttttgcctta ataacagcct tacattaaga
    17221 attgatacat attattcaag caattattat ttatcggaca agagccaagg aacttatggt
    17281 ccatatcatg cagtgctgca ggatgaaata tattcttcct atgaagaagc ttatttgata
    17341 gcagaagaat taaatgaaga gtttaaggta gagttttatc cttatctgat tgaagacgga
    17401 tatattattg ccggaggaaa ttttgcggat aaaagctctt cacaggattt agcggaagaa
    17461 ttaaatgatg agggctatat tgctgaatcg atgaatggca acctggaaaa cataattgca
    17521 tacaacaaaa agaatatacc tgtattcatg tataaaaagg acatgcctgt ttatttttct
    17581 tcgtacaacc aagatgaaga atatgaaatg attaaggttg acaacagagc ctacagagga
    17641 agaataagac tgaatataat tgaagacttc aaacttttaa ccataaatca tgtagagctt
    17701 gaaaactacc tctatggagt tgttccgaat gaaatacctt catcttgggg aatagaagca
    17761 ttgaaggctc aagctctggc cgcaagaacc tatgcggtgt acaacataaa gccaagacaa
    17821 atctacgata tggaagacaa tcaaaacagt caggtatata tgggttatga ctatgaaaag
    17881 acatctacaa acagagctgt ggatgaaaca aggggagaaa tgatttatta cgacggcgaa
    17941 ttaattcagg ccttctatca ttcaaccagc ggcgggtcta cagaaaactc cgaaaatgta
    18001 tggtatgaaa agctgccgta tttaagaggt gtaaatgacg aattttccaa taattcaggc
    18061 tcacctcata ccacctggca gaccacgtat tacaaggaag acataataag aaatttaaat
    18121 cttgatggca atgacgtaga tgaattgtac ggaattcaaa taaagaaagt ttctgaaaat
    18181 aacagggtag ttgaatgtat atttttaacc gatgcaggag aaatttcata taagaaggaa
    18241 aatgcaaggc ttcttttggg attgaaaagc tcgtggtttt ctatagtaaa cggaaatata
    18301 ttttgcttta taacagaata cttttcttta gatgaaaatc gaaaagatga aacaccgcca
    18361 tcaagaggag tacttgattt aataacagaa gaggaaacag aagaaacaag aaatgaaata
    18421 gagtttgaaa ccggaagtat tgcaggtaag catgcaataa ccgacgacgg aataaaaaaa
    18481 ataaacaggg aaaaattatc ctttataagt tcaaatggtg taacaacagt agacgctaat
    18541 tcagagcaat ataattttga aggaagaggc tggggacacg gtattggaat gagccagtac
    18601 ggagctaagc aaatggctga agaaggatat acctatgaag aaataataaa acattattat
    18661 accggagtaa cagttaaatg acgaatttta aaacaagcga cttttattac gaattgcccg
    18721 aaaggctcat agcacagaca cctattaagg aaagagacca ttccaggctt ttggtacttg
    18781 acaaaaaaag cgggaaaatc gagcataagc atttttttaa tttagttgaa tatttaaatg
    18841 caggagactg tttagtaatt aacgatacca aggttctgcc tgcaagatta ttcggaaata
    18901 aaaaagatac gcagacttta attgaaattc ttttactgaa aagaatagat gataaaaagt
    18961 gggaaatatt agttaagccc ggtaaaaagg cgagaacagg gacagaaatt gtttttgatg
    19021 aaaaattgct tacggggaaa attatcgata tccttgaaga aggcaacaga ataattgaat
    19081 ttgaatatga gggcatattt gaagaaatat tggatagatt aggcgaaatg ccgctgccgc
    19141 cctatataca cgagaaactt aaggataagg acagatatca gactatctat gcaaaacact
    19201 tgggctctgc agctgctcca acggcaggac ttcattttaa tgataagctt ttggaagaaa
    19261 ttgaaaataa ggatgtaaat atagcaaggc ttaccttgca tgtggggctt ggaacattca
    19321 gacccgtaaa gaccgaatac atcaatgagc acataatgca ttcagagtat tatgaattaa
    19381 acgaaagtga agcaaataaa atcaatcttg ccaagagctc aggacacaag gttataaccg
    19441 taggtaccac aagtgtcaga acattggaaa cagttgctga tgacgaagga atagtaaaca
    19501 aaagcaaggg atggacgaat atttttattt atcccggata caaattcaag gtggttgaca
    19561 gtttagtaac aaactttcat cttccggaat caactttgat tatgctggtt agcgcattgg
    19621 caggaaaaga aaatattatg aaggcatacc gtgaagcagt agaaaaagaa tacagatttt
    19681 tcagctttgg tgatgcaatg tttataaaat aggtggatta tgtttaaatt tgaattaata
    19741 aaagaatcta aggaatgcaa gggaagactg gggagaatat atactccaca tgggattata
    19801 gaaacacccg tattcatgcc tgtcggaaca aagggaacgg ttaaggcaat gacacctgaa
    19861 gaattgaaac agctaagggc tgagattata ttaggaaata cctaccactt gtatttaaga
    19921 cccggtcatg aattaataag agaagccgga gggcttcaca aatttatgaa ctgggacggc
    19981 cccattttaa ccgacagcgg cggattccag gttttcagtc tggagaattt aagaaaaata
    20041 agggaagaag gcgttgaatt ccgctcgcac atcgacggct caaagcattt cataagtcct
    20101 gaagtttcaa tggaaataca aaattattta ggctctgaca taatgatggc atttgatgag
    20161 tgtgcaccat atcctgcaga ttatgaatat gttaaaaagt ccatggaaag aacaagccgc
    20221 tgggcaaaaa gatgcaagga ctatcacaaa aatacaaaaa gccaagcctt gtttggaatt
    20281 gttcagggag gaatgtacaa ggatttaagg attgaaagtg ccaaacagat tacggatatt
    20341 gagtttcctg ggtatgcatt aggaggctta agtgtaggag agccgaggga tttaatgtgc
    20401 gaagttatgg attataccgt agagcttttg ccgaaggata aaccaagata tttaatgggt
    20461 gtgggcagcc ctgattattt gtttgaagcc gttgaaagag gaatagatat ggcagactgc
    20521 gtcttgccta caaggatggc aaggcatggt gcgtttttaa caagcaaggg acagcttaca
    20581 attaagaatg caaaatataa aaatgatttc agtcccattg atgaggaatg cagctgctat
    20641 acttgcagaa attattcaag agcatatata aggcatttat ttggcgaaaa agaaatattg
    20701 ggcgctaggc ttgcatcaat tcataattta tttttcttga ttaatttaat gaaaggtatt
    20761 agaataagta tagcggaaga taattttatg aagttcaaaa gagaatttta tgagaattat
    20821 ggatattaaa aaagtaaata ggaggaatta tgccacagga atatagcggt attttaatgt
    20881 tggtcgtttt ctttgcgata atgtatttca ccattataag accacagaaa aagagagaaa
    20941 aagaaactaa agcaatgaga gacagcttgg caacggggga tgaggtaata accataggcg
    21001 gtattcacgg aaaagttgtt aaaatcaatg atgaaattgt tacattagaa atgccctttg
    21061 gtaaacaaag gattgagttt tctaaatggg caataggaag tgttacaaag aagggcaaag
    21121 aaaaagcagc taaagaagaa gttgttaaag aagaaattga agaaactgtt gatgataaag
    21181 aagatgacaa ataaaaaaga gctgatgctc tttttttaat tgtcatcctg agcgccagcg
    21241 aaggatctca tgacaaaaat gttattaatg tctatgacac tgacttatag caataatggg
    21301 ttatttctta aaaattttct tccccataac aattacttcg tgtgacagta aaggaacagc
    21361 tgatactaat atcagatttg cccattcttt caatgaaagc tggcttgttt caaacacttc
    21421 ggtaaggaaa tgaacttccg ttacaagtat ctgcagcagc aaacctaaag aaaatgctcc
    21481 aatcattgca agattatcgg tgtggctcat ttggaacaat gatttgttat aatttctcat
    21541 acctatggca tggaaaagct gagaaacacc aagagtcgta aatgcatatg tttgagcatg
    21601 cctaataata tcaggcaaat ctactccggg tatactaaga acaccgttaa ataaatcacg
    21661 tatatttccc aaggttacag gtattcctgc ttcattcaaa tgaatgacag gtgaccataa
    21721 aaaggctgca agagtcaatg atgcaatcat tattccgttg taaacggtaa atatcaaacc
    21781 tcccttagca aacaagcttt catttgggtc tcttggtttt tcattcatta tttctttgct
    21841 cttcgggtca actcctaatg ctaatgcagg aagcgagtcg gttatcaggt taacccaaag
    21901 aatgtgtatg gactgcagcg gtgctatcag tcctgccgca atagatgaaa acattgatat
    21961 aacttcacca aaattggagg ataacaagaa tagaaccgtc tttttaatat tattgtaaat
    22021 acctcttcct tccgcaactg ccttttcaat ggttgcaaaa ttatcatccg taagaaccat
    22081 atcagaagca ccttttgcta cgtcagtacc tgttatgccc atggcaacac caatatcagc
    22141 tgattttaat gatggagcgt cgttaacacc gtcaccggtc atagaaacta tgcttccgtt
    22201 tgatttaaat gcttttacta tcattacctt gtgttccggt gaaactcttg caaaaactct
    22261 tatatcacaa actttttcat ttaattgctc ctgagtcatc tcattcaatt cactgccggt
    22321 tatgcattgt gattcatctt ctgcaatacc gagctccttg gcaatagcaa gagcagtatc
    22381 cttgtggtct cctgtaatca ttatagtcct gatacctgca cttcttaaag tttttaccga
    22441 atcctttgct tcgggtcgag gagggtctat cattcctacc aagcccacaa atgtgagatc
    22501 ttcttcgctt gctgaatcgt catcttcttt gattccaaga gccaatactc taagagctcc
    22561 ttttgccatt tctgtggcag ccctgtttat ttcttcaatg tgttcatttg ttatatcttt
    22621 tgtttcgccg tttatatata ttttattgca cctgttcaaa agtatgtcca tggcaccctt
    22681 agtgtagctc attaccttgc cgtcttggtc cctatgaact gttgtcatca attttcttgc
    22741 agaatcaaag gattgctcat tgattctcgg attagttttt tctaatcctt cccttgacaa
    22801 attaattttg tctcccatat ccaagagagc taactctgta gggtctccta tacgagtgtt
    22861 attagctatg gaggcatcat tgcaaagaat aaagccgtcc aaaaatattt tgttgttttc
    22921 ataagataag tcatcggtat ttttaatttc accgtctaaa tatacttttg ttacggtcat
    22981 tttattttga gtgagagtac cggttttgtc tgagcataca atgctgactg ctcctaacgt
    23041 ttcaactgcg ggaagcttgc ggactatggt atttaccctt accatttttg taacaccaag
    23101 agccaatacg attgttacaa ctgcagtaag cccttcaggc acggcagcta cggctaaggc
    23161 aatggctgtt ataaacattt cgattatgtc tcttccctgc agaacagcta ttccaaacat
    23221 tgctacaact ataactacgg caactatacc taatactttt cccaagtcat tgagtctttt
    23281 ttgaagggga gttaattctt cggtgctttc acttatcatt gcagctattt ttcctatttc
    23341 ggtatccatt ccggtataaa ctacaacacc ttcaccacgg ccgtatgaaa cacttgtgga
    23401 agagaatgcc atatttaggc ggtctccgat tcctacttcc ttgtcagcca ccatatcggc
    23461 atttttgtct acgggtacgg attcaccggt taaagcagat tcttcgattt ttaagtttac
    23521 cgactgaata agtcttaaat cggcaggaat aattcgtccg gcttcgagta ttactaaatc
    23581 tcccacaacc agctcacttg caggtatttc cattaccctg ccctcacgcc ttaccattgc
    23641 ataggggctt gaaagattct tcaaggcttc aagtgcagct tctgcttttg attcttgaac
    23701 catacctatt actgcattca ataaaacaac taatagaatt attattgcat ctgttatttc
    23761 gtttagagct atggatattg ctgcagctgc aaacaatatg taaatcagtg tatcttttag
    23821 ttgggccaag aacatctgca gctttgtttt tgcctttttc tcaaccaatg cattgggacc
    23881 gtctgacaac agcctctttt ttgcttcttc ttgggttaaa ccggtacttt gagcggtgtt
    23941 taattcttcg catgtttctg ttatgctttt tcggtaatac attgtctact cctttaagat
    24001 tattcacaac tgtttaataa aaaagacttt tagcatacct actccagata taccaaaagt
    24061 ctcgtttcct aagttgcccg aggattttca gccagaatac atatattccg tgttgttgac
    24121 tgaaaatttc aaactactcc cttttagcaa gataacttta tcaaatatag aaatctttgt
    24181 caagtaatat attaatgttc ttttttatta tcggatgaat attatttagt aagtcatgct
    24241 caaggaggta ttatgaagtt aggtaaattg tttaagtatt ctctgttttt atttatttta
    24301 tctttaataa tttattttat actgaccgtt tatatatatt atttttcaaa taatcccggt
    24361 tttgaaatag tctacagctt cattgttcca atatgcatat ttgctgtttc aatgttgtat
    24421 tcaagaagtg ttcatgaaaa agggctgtta aggggaattg aaatttggat agtatatttt
    24481 gcaatggtct tattgataaa agtcttattc agatacccgt ccgaaataag aatactatac
    24541 aacggggtga ttctaataat gtcaatactg ggaggaatca tcggtgttaa catgaaaaac
    24601 aagagtataa aaaataaata gtgctcttgt ttaaaaaaat aaaaaaaata ctttaaaata
    24661 acctcaacta atgctataat ctattaagtt tataaggagg ttgtcatgaa atacataaaa
    24721 acacttaata attataagtt aacggataat ttaagcgcaa agggatgcgg agaatgccag
    24781 acatcatgtc agtcagcttg caaaacatct tgtactgtcg gaaatcaaaa atgtgaaaaa
    24841 gaataattgt agtatagttt attgtttaga cagagtttgt cattctgaag gcgcagcttg
    24901 agaggtgctc taacaattag aggcagcaca ctacatagct tcgttcagga tgacttattt
    24961 ttaattagca aaatttcgga ggaagtatga tacatgtatt taatataaaa gatttatatt
    25021 tcgcaacaga tacaagcacc ggactggtac atgccgtcga taagatagta tttgatttat
    25081 taatgaatga aaaattcaag gatgaaaata aatacagtga gttatacaat gaatacggag
    25141 aaagcacagt tagagaagct ttatcggaaa tacaatattt aatagacaat aaaatgctct
    25201 ataccgaaga tattaaatat gtaaatacaa taaagccggt tattaaagca atgtgtttga
    25261 atatgaccca cgactgcaat ttaagatgcg aatactgctt tgcttcacaa gggacatata
    25321 acggagaaaa ggcatttcta agttttgaaa cagggaaaaa ggcatttgat tacttggtga
    25381 aaaactccgg taacagaaaa aacttagaag tagatttttt cggcggagaa cctctcatga
    25441 attttgacgt aatcaaaaaa ctggttgatt acggaagaag cttggagaaa gaatataaca
    25501 aacatttcag atttaccgtt acaaccaacg gtgttctttt agatgaagaa aaaatggact
    25561 atattaatga aaatatggac aatgtggttt taagcatcga cggaagaaaa gaaacaaatg
    25621 acagaatgcg taaaacaata aataaaaaag gaagctatga cttaatagtt gataattata
    25681 agagattcat cagcaaaaga ggcagtaagg attactttgc caggggaaca tacacttcca
    25741 ataatttaga cttctcggaa gatgtcaagc atatgagaga attaggtttt gataaaattt
    25801 ctgtagagcc cgttgttgct aaggatgagg aaaaatatgc actaaaaaaa gagcatgtgg
    25861 atattttaaa aaaagaatat gaaaaattag ctgaatatta tattgaatca tataaaagta
    25921 aagatagaag gtttcagttt ttccatttta atatcgaact ggagggaggt ccctgcattt
    25981 acaaaagatc cattggctgc ggagcaggaa ctgaatatgt tgccgtaacc ccgtcaggtg
    26041 atttgtatcc ttgtcatcaa tttgtaggta atgaagaatt cattatcggt aatgttgagg
    26101 aaggtattac aaatcgggct cttgccgata aatttaaaaa tgtatcggta aatgataaac
    26161 ctgcgtgcag agattgttgg gcaaaatact tttgcagcgg tggttgtcat gccaatgcat
    26221 ataattttaa caaggatttt actgtcccat atgatgttgg atgcgagtta gaaaaaaaac
    26281 gaatagagtg cgcaatttat ataaaagcta aaataaatta agactttcct tcaattaaag
    26341 gttgtcccca tacctgtaaa aaaaacagtg gtaattgtat atggtaatat agtataatac
    26401 agatataata aaaataaaga caagagagga gtattaatat gcaaattggt tttatagtaa
    26461 ttcttataat tgcgattttt gttgcgattt ttgcaataca aaatggaaca ccggttcctg
    26521 ttgatttatt ttttgcaaga tatgaaatgc ccttagctgt aataatgatg atttgtctga
    26581 ttcttggtgc cgtggtagtc ttaatattag gtactaccag acaatttaag aaaagatccg
    26641 agtataatga attaaagaac aaactcaaaa ctcttgaagg cgaaaaagct aagcatgaaa
    26701 tagatatgaa agcattagaa tcagaaaaag ctcaatttca aacaaatata gaggctcttg
    26761 aaacagaaaa acaaaggctt cagaatgaaa tagccgattt cagtaaaaaa gttacactgc
    26821 ttgaagacaa aattaaattg caggatgaag aattagttgc taaaaaccaa gaaatacaaa
    26881 ttttaaaggc tcaaaatatt gaaaaaattg aaggggatac ggtaattaca gatgaaggtg
    26941 ctgcacagat agacaatgaa accagtgaag ttgaaagtca atgaatataa taatgatgaa
    27001 atagaaagaa tcagcaaaag gtttaatctt tcaaaaacat ctgcaaaaat acttttaaac
    27061 agaaatataa aatcttttga tgatatagag caatttcttg aaccgaactt caaatattta
    27121 gaaagagctg aaaattacaa agatttacat aagggctgca aaagagttat tgaagcaatt
    27181 aataataatg acaggattct gatttacggc gactacgatg ttgatggagt tacttccatc
    27241 agccagtttg ttgttttttt aaaaaaagca ggtgcttatg tcaattatta tgttccggaa
    27301 agagaaagcg aaggatatgg aataagctcc gattttgtta acagtgttaa aagcggtgaa
    27361 ataaattttg agcttctgat aaccgtggat tgcggcatag ctgaaattga aaaaattgac
    27421 gaaattacaa agcttgataa agatgtaatt ataattgacc atcatgagag cagggaagag
    27481 cttcccaatg cttatgccat aattaatccg aagcaaaaag attgtccgtc cgaaaacaag
    27541 catttatgtg cagccgggct atcttttaaa tttttgataa atttaaataa gtttttaaaa
    27601 atagataata ttgaggatgt gctcctggaa tatgcatgtc ttggcactat tgccgacata
    27661 gttgatctgg taaaggacaa caggattatt gccagtcaag gtttaaaaaa aataaataaa
    27721 actaagatta tagggcttaa aaaattaatt gaagtatcag gcataaacgg agaaattaaa
    27781 tcctatcata ttggttttat acttgcccca aggattaatg cctcaggaag aatggatact
    27841 gcaaaaaaag caattaagct catgcttaca aaagatgaag aagaagcaga aaagcttgca
    27901 tctgagctgg acagtttaaa caatgcaaga aaagaagcag aaaatataat atataaagaa
    27961 gcaataaaga aaattgaatc tgatttcctg tataaaaata atgtaatggt agtatacggg
    28021 gataattggc atgaaggtgt gcttggcatt gttgcctcaa gacttactga aaaatattat
    28081 aagccctgtg ttgttatatc cgtaaaggat gggataggaa aaggttcagc tcgaagtgta
    28141 gagcatgttg atatatttga atcatttaag catacagata tttatctcga aaagtatggc
    28201 ggccataaat tagcggcagg tcttaccatt ttggaaaaaa atattaattc cttcacaaat
    28261 gaactgaaca attatatcgg ctccactact gacgatgaat ataatcaaat tagagcagat
    28321 gcaattctga atatatcaga cataaattta aggctttatg atgaaataaa cagatttgag
    28381 cctttcggtt caggcaatcc gaaacccttg ctagccctaa gagatgtgtc gataaaaaat
    28441 ataaggaaag tgggcaaaga agggaaacat atatccttta tgctgtctga cggagacagg
    28501 cacattccgg taatagggtt ctgcaaaatc ggaatacttg aaaaaatact taccatgcct
    28561 caatcctaca ttgtatcttt aagtgaaaat atatataacg gcagcagaag cttacagctt
    28621 tttttacatg acgtagagga gcatgaagaa tttgattaca aaattgatgt taataaactg
    28681 aaagcattgg agtttctgat taacaaaaca aaatccaaaa taattaagac ggatttattt
    28741 atacttgtag aaaaacttaa taaaagatat aatacaaaaa taacagcaga ggaaataata
    28801 tgcatgttga aaaaggctga taatattcag tttgctttaa aaaatgacat attgtatata
    28861 aaaaaataaa tgcttggagg aagaaatgga tttaaaggaa aaaattcgtg ttatagagga
    28921 tttcccggct aaggggatta gttttaaaga cattacaacc ttgctgaaag attctgaagc
    28981 tctgcatgaa tgcgtcaagg aaatggcaga aaggtttaaa gatgttcatg tcgacataat
    29041 tgcaggaccg gaatcaagag gctttatatt tgcaacacct cttgcatatt tattaaaaac
    29101 aggatttgta cctgtaagaa agcccgggaa actgccggca gaaactatta aatatgaata
    29161 tcaattggaa tacggcacag attctttgga aattcataag gatgctatta aaaaaggaca
    29221 gaaggtatta attattgatg acttgttggc taccggagga accatgtacg ccactgcaaa
    29281 actggttgaa aagttaggcg gcgaagttgc cggacttggt tttctgatag agctgaagga
    29341 tttaaaagga agagaaaaac taaaagaata cagggtggaa tcattaatca catatgataa
    29401 ataaaactgc gagactgcct gcggataaga cgaatgtcct aaaaaatttg tgcactaggc
    29461 tgcatgtaaa ggaacttagg tgagttatgt taggcaatat tattagtcaa atcgaatcat
    29521 acaatccaaa tgctgatttg ggcattgttg ctaaggcata taattttgct gaaatggcac
    29581 attcaaatgc acaaacaaga ttttcgggtg aaagatattt tattcaccct tataatgttg
    29641 cgctcatttt ggctgactta cacatggatg tacagactgt ttgcgcaggt cttttgcacg
    29701 atgtagtgga agatacggga ataactttcg atgaaattaa aagggaattc ggcgaggaaa
    29761 ttgccaacat ggtggatggc gtcacaaaac tgagcaaggt taagtacagg aacaaagaag
    29821 agcgccaagc agaatcttta agaaaaatga tagtggcaat gtcaaaggac ataagggttg
    29881 ttataatcaa attagccgac cgacttcaca atatcaggac attacaatat atgcccaagg
    29941 aaaaacaata ccaaaaggca atggaaacta ttgaaatata tgcacccata gcaaaccgtc
    30001 ttggtattgc gtccattaaa tgggaattgg aagatttgag cttaaagtat atagatcctg
    30061 aaggatatga agatttgctt aaaaaagttc aggaaaaaag cgaaaaacga aaagattata
    30121 tttcgcacat tatcgatatt ataaataaaa agcttcagga atcgggaatg gaagccgaca
    30181 ttaagggcag acctaaaagt ttatacagta tttacaaaaa aatgtattat aaaaacagac
    30241 tatttgaaca aatctatgat ttaattgcca taagaattat tgtggaaaat ataaaggatt
    30301 gttacggagc attgggtatt gttcatactc tttggaaacc ggtgccggga agatttaaag
    30361 attatatagc catgcctaaa ccaaatatgt atcaatcttt gcatacaaca gtaataggtc
    30421 ctgaaggcga gcctttcgaa attcaaatca ggaccttgga aatgcataga actgctgaat
    30481 atggtattgc agcccactgg aaatacaaag aaggtataga tgataaaacc aagtatgaag
    30541 aaaagttgaa ttggttgaga caaatgcttg aatggcagca ggagactaac gaccctcagg
    30601 aatttatgga agctttgaaa attgacttat atactgacga agtatttgta tttactccaa
    30661 agggagaagt gataaatttg cctacaggat ccacacccat agattttgct tacagagttc
    30721 atactgcagt aggaaacaag tgtgtgggag caaaagttaa cgggcgaatt gttccgctcg
    30781 atgcgaagct taaaacaggt gaccaagtag aaatattaac atcccagtca agtgtgggac
    30841 caagcaggga ttggctgaaa attgccgcgt caagtcaggc aaaatctaaa atcagaaaat
    30901 ttttcaaaga tagagataaa gattttaatg ttgaaaaagg aaaagaatta gtagaaagag
    30961 aaattaaaaa gcagggatac caagtagcag atttgcttaa ggatgactgg attaaaactg
    31021 ttgccgacaa atacaatatg tctgctgcaa gcaacctctt tgcggcagta ggcagcggag
    31081 gtataacaga aagccagata gtaaacaggc ttaaattaat atatcttgaa aagaacaaag
    31141 ataaactcga acttgataga attgaaaatt tagaaaagag tttggcaaaa tcaccccaga
    31201 gggaaagaca tacatccggc gttataataa aaggtattga taatataaaa gttcgttttt
    31261 ccaaatgctg caatccggtt cccggagatg ccatagttgg atatatcaca agaggcagag
    31321 gagtatcaat tcacagggct gactgcacaa acattcatga tttaagcgat tctgacgacc
    31381 agagatttat tgaggttcag tgggataata aagaaaaggc aacattcatc agcgaactac
    31441 agattaaagc gttggacaga ccgaaacttt tgcaggatat aacttctttg tatgtagatg
    31501 caaagcttaa tgccgtatcg cttaatttaa gaataaataa ggaaaaagta gcaatcgttg
    31561 atatcggatt tgaaataaat gatattaaac aaatagaaga tctgattaaa aagatgaaaa
    31621 agttagacgg agtattggaa gtctacagaa ataaaaagtg acgaaaaaat gaaaggatga
    31681 aaaatgagag cagttgtgca gcgcgtaaaa agcgcccacg ttaaagtaaa ggataaaata
    31741 ataggaagca tcggtcaggg aattttgcaa tttctcggtg ttgaagaaac agaccaagaa
    31801 acggatttag aatatatgtg tgaaaagatt ccaaatctta gaatatttga agatgaaaac
    31861 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatgacgg tcctgttacc
    31981 atacttcttg acagcaaaaa agttttttag gaggttctta tgatatttga agcattggaa
    32041 gtcggtattt attgtgcaaa ctgttatata gtcggaagtg aagaaactaa ggaagcagcc
    32101 ataatagacc ccggtgctga ctttaataaa attgaaggga aactgcatga attgggatta
    32161 acccctaaaa taataatttt gactcatcat catggtgatc atattggagc ggtgaaagaa
    32221 tttaccgata aatacggatt gaaagtttat attcataagg aagatgcgga agcactcaat
    32281 aacagcagca tgaatttcac taaatcaatg ttcgggaaga atattttaat aaaggcagat
    32341 gttgagttag aggatgggga cgtaatatac ttaggtgaat taaaatttga aataattcat
    32401 actcccggac atacaaaggg aggtatttgc atcaaggttg gaaatataat gatgacggga
    32461 gacactttat tcaactcttc cattggaaga accgaccttc ccggaggctc ctatgaagaa
    32521 attataagct caataaaaga aaaaatattt aaattcgatg atgatactgt aatttatccg
    32581 ggtcacatgt caccttcaac aataaaaaaa gaaaagaaat ttaatccgtt tgtaaaataa
    32641 gatgctatca ggtagaaaaa tgtttaagtt ttattttttt ggtcatgatt acgaatatga
    32701 agctagaaat gcgctcagag tattcgattt aaacatagat tatgaaataa tgcatggctc
    32761 ctttgacgga aggagcttat ctgttttatc atatcttgaa gaaggcgaaa ttattagtgc
    32821 aaaggcacag ctttataatg actgtgaatt gctgtatgaa tcatgtatca gtgaaaatga
    32881 catcgttttg gagaaataca gcttaaaaaa gctgaaaaaa acattggtaa ttaaaactat
    32941 acacaatgtt ttgaaatcat attacaaggt ctatcccgat tatggaatat taaccggagt
    33001 cagggtggta aaaattttaa tcacagcaaa aaaatactct aaaagcaacg aagaaataaa
    33061 taagatactg agagatacct atgaagtgaa agaggaaaaa ataaagcttc tttgggatat
    33121 attaaatata gaagaaaaat atattgatga aaatataaat aaaaaaaatt acaacttata
    33181 cataggtatt cctttttgcc cttctaaatg cagctattgc tcctttacct catatgtaaa
    33241 ttgtactgat aaaaaaatat ttacctactt ggatactttg atatatgaaa ttgaaaaaac
    33301 aattgaactt gcaaaaaaga aagaattaaa cttacatacg atttatatag gcggcgggac
    33361 tccttcagta ttaaatgaag agcagataga aacaattttt aatgcgataa aaaaatatta
    33421 cgatttgtct caagtaaagg aaattacttt tgaagcaggt agaccggata ccataaatag
    33481 tgaaaaactt atttgtctta aaaataattt tgtaaacaga atatccataa atccccaaac
    33541 gatgaatgat aatacattat tatcaatcgg aagaaaacac ttgggctttg aagttgttga
    33601 aaagtactta ttagctagag aaatcggatt tcaaacaata aatatggata ttattttggg
    33661 actgccggga gaagatgaat cagatgtaaa aaatacaatt gaaataattg cggggttaaa
    33721 tccggaaaat attacagtcc actcattggc atataaaaaa cactctgagc ttaccagaga
    33781 aagtgcaaaa ctttttaagg attatgaact tataaaaaaa atgcaccatg ttataaagga
    33841 ggtttgcgaa gttaaatgtt acaaaccata ttacatgtat agacaaaaga acatcaaggg
    33901 caactccgaa aacatcggct atacaaagaa agacagggag tgtatttaca acattgtaat
    33961 gatagaagaa ttagaaacaa tacttgggtg cggtcttggt gcttcaagca aaataatgac
    34021 aggtgaaaac aagcatgaaa ctgtacgcaa ttttaaaagc ctggaagaat attcaaagag
    34081 aattgatgaa ataataaata aaaagcaaac tctattagga ggataaatga aaaaaataaa
    34141 acttttttat atagaaggct gtcctcattg caggaaagca tttagaatac ttgatgaatt
    34201 gaaagctaaa aatcccgaat actcaaaaat tgatgtggaa tacatagatg aaaacaagga
    34261 tgtagtatca gcaaatttat atgattatta ctatgttcca acattttttg tggacgatgt
    34321 gaagatgcat gaaggggtac cgacggaaga aaaaattgaa agagtattga aagaggcaat
    34381 aaaataaatc aaaaaatcag gacagctcgt cctgattttt tttcaataaa attatttttc
    34441 acagtgcttt gcatctgctt ctgtttcaaa atgaataata agtgctattg ccgctctcag
    34501 taatactaag gctgcaagtg ttatcaattg ttcttttgaa tgtgctatta aagatttaag
    34561 aatttccgcg cccagcatat atgccaaagc catttccaac gcccttgaaa attctatttt
    34621 agcttgttta ttagtaaatt cgaatttgct cattagatat tttattaatg ttgttactgc
    34681 actgtaagca acaattatta tgcttacaat ttctaaggtg taagcgacat aatgaactgc
    34741 ggtttccatg aagtgttcca attaaaaacc tccaaattta tattatattt aacgtatgct
    34801 attatattgc attttattaa taatgtcaaa gcccttttag agtgattttg gaaatatgca
    34861 tcctattata aaaaagaata ataaataatt tatagccagt catattaagc ctgtttattt
    34921 atataaataa ataaaggggt gagatatatg gtattaagaa taaacaaaaa ggtatttatt
    34981 attataatga tagtagtttt attgtttgca tgtttattga tttacaatac ttttaagaat
    35041 ttttcggtga cagtttcaac aaatatatcg aattgggggc taaaattcaa tgaaaacgga
    35101 acagcaccaa tgggtaatgc aggaaaagat tttttaaaga aatataatgc ctattatgta
    35161 ggggagccgg ataaaaagta tatttatctc acatttgatg ccgggtttga aaacggaaat
    35221 actcccaata tacttgatac attaaaaaaa catgatgtga aggctgcatt tttcttagtg
    35281 ggacactatt tggaaaaaga gcccggactt gtcaaaagga tggttagaga gggtcatata
    35341 gtaggtaatc atacctacca tcatcccgat atgtcattga tttctgacaa ggaatcattt
    35401 aggagggagt taacatcctt agaagaatta tacaaggaaa taaccggtga agagatgcca
    35461 aagtattaca gacctcctca aggcaaattc agtgaagaaa atttaagaat ggctgatgaa
    35521 ttgggatata aaacgatttt ttggagcctt gcgtatgttg actggtataa ggatgaccag
    35581 cctaccaagg agcaggcatt cagcaaatta attccccgaa tacatcccgg agcagtagtc
    35641 ttattgcact ctacctccga tacaaacgaa atgatattgg atgagctgtt aacaaagtgg
    35701 aaagatttag gatatacatt taaaacattg gatgatttaa cagcaaacta aatgacaagg
    35761 ggacgggggt actgtcatca aaatttaatt ttattgttga caaacaagat ggggtgtggt
    35821 aatttagttg taaataattg caggggaaga gtagtcataa agcaatgatt taagcaagac
    35881 agggacgatg ggagcctgtt atcaactttt gatgaagaca cctcaaaaat gcaaattcta
    35941 atgagtggga cttattagtc caaatagggt ggcaacgcgg gttatactcg tccctaagca
    36001 ggggacgggt ttttattatt ataggtattg ggacacacct ctttcaataa agattgcctt
    36061 tgccggtcag aggaatgtcc ctacttaaac aatggaggat actatggaaa cgatgggaac
    36121 acttagaaga acaaatatgt gcggagagtt gaataaggaa cacattggca aggaagtcgt
    36181 gctgatggga tgggtacaaa aaagaaggag cttaggcgga cttatttttg ccgacttaag
    36241 ggatattacc ggattaattc aaattgtttt tgatgctgat gtaaataagg aagcttttga
    36301 aaaagcagat agtttaagaa gtgaatatgt aatagcaatt aaaggaaaag tatttgaaag
    36361 acagtctaaa aaccttgaaa tggctacggg agagatagaa gtttacgcac aggagctaag
    36421 aatattagat actgctgaaa ctcctcccat ttatataaag gataatgatg atgtttccga
    36481 gcagatgagg ttaaaataca gatatcttga tttgagaaag ccttcaatgc aaaaaaatct
    36541 attgatgaga agtaagacat caaaggttat aagggacttt ttatatgaaa acgggttcaa
    36601 tgaaatcgaa acaccatttt tagcaaaacc tacacctgaa ggagcaaggg actatctggt
    36661 accaagcaga atttcacccg gtaatttcta tgcattgcct caatcgccgc aaatttataa
    36721 gcagcttttg atggtttcgg gaatgaacag atatttccag attgtaagat gcttcagaga
    36781 cgaagactta agagcaaacc gtcaacctga attcacacag gtagacattg aaatgtcctt
    36841 tgtggatgca gaagatgtaa tggcgataaa tgagaggctt attcaaagaa tattcaagga
    36901 agtaaaaaat atcgatatta agcttccatt aagacgtatg tcttataatg aagctatgga
    36961 taaatacggc tgcgacaaac ctgatttacg ttttggatat gagctgaaaa atattactta
    37021 tgtgtttaaa aattctgaat ttaatgcatt caaatcagtc tatgaaaaca gcggcttgat
    37081 taaatgcata aaaattgacg gagcatcgga tgattattca aagaaaggca tttctaagtt
    37141 agagaaattt gtaaaatcac acggtgcaaa aggattagcc tggtttaaat atgaaaacgg
    37201 cctatttgat tctccgattg caaaattttt aagtgaaggc gaaataaatg aactaatttc
    37261 tttgttggag ctaaaggaaa aagatatagt ttttatagtt gccgataatg aatctgccgt
    37321 aaatactgcc ctcagcgcct tgagaatcga aatagccgag gaaagaaatt taattgacaa
    37381 taatgtttat gaattagtgt ggattactga ttttccactg tttgaataca gcgaagaaga
    37441 agaaagatac gtggcaaaac accatccatt tacatcacct gtagatgagg atatagataa
    37501 attagaatca gctccggaag aagtgagggc aaaagcatat gacatcgtaa taaacggtga
    37561 cgagattggc ggcggaagca tacgtataac aaacagggat ttgcagaaca gaatgtttaa
    37621 agcattagga tttactgaag aagaagcaca aaaaaaattc ggttatctgc ttgaagcatt
    37681 taaatacggc gtgcctcctc acggcggaat tgcatatgga ttggaccgct ttatgatgct
    37741 tttgacaggc gcaaacagta taaaagatgt aattgcattt ccaaaaacac aaagtgcttc
    37801 ctgcctgatg acagaatctc ccagccaggt atctgagaag cagcttgatg aactgaaaat
    37861 tgaaattaat gagtaagttt tataggaagg aagatcaaat ggacaatata ggaaagattg
    37921 aaaaaattga cggtaacatg gctgttattt ctgttaaaag ggtatcatcc tgcggggaca
    37981 gctgcaaaag ctgctcttcc gcatgcaaag taaacagcgt tattatagaa accgatattt
    38041 cggatgtaaa agatgaaata aatgtcggag attatgtgga aattcgagcg gaaaatgaag
    38101 tgatgcttaa gcatatagca gttctttacg gacttccttt attattgatg ttagtaactg
    38161 tttttatttt tcaaatgata ttaaagggtc ccaataagga tataatctct gcagtagctt
    38221 cattgttttc gttaataatt tctttttacc tcttaaaggc ttacgataag agtgaaatga
    38281 aaaagaatgt attaaaattt actgttggta aaaaacttta atgcatacga tcaggatgac
    38341 aaaggtcatc tgggcgcagt gatgaatccc gtgaggcgtc attctgaacg cattgaagaa
    38401 tcataaggat tgatacaatg aatatatttg aaatgcaata ccaaaattcc ataaaaaaag
    38461 aaggtcctct cgcagagcgg atgagacctt taaatttaag tgaatttgtc gggcaagagc
    38521 atattgtggg cgaaggcaaa attttaaaca gagcaattga agctgacaag cttacctctg
    38581 cgatttttta cggacctccg ggaaccggca agacaacctt ggctaaaatt attgcaaata
    38641 ccacaaacaa taattttacg actcttaacg ccgttacaag cggtattaag gatataaagg
    38701 aaaaaataag ctatgcagtt gatgagcttt caatttataa taagagaaca attcttttca
    38761 tagatgaaat tcatcgtttc aataaagctc agcaagatgc cttgcttccc tacgttgaaa
    38821 atggtacaat aatattgatt ggagcaacca ctgaaaatcc gtattttgaa gtaaattccg
    38881 ctttgatttc acgctctttg gtatttgaat tcaaaagaat tgacatagag gatatcaaga
    38941 atgtggttag aaaatcattg gtggacaaaa gaggatatgg aaacagaaaa gtggaaatcg
    39001 atgaagacgc cttagctcat attgcagaat acgcaaacgg agacataaga agcgctctta
    39061 acgcattaga gcttggtgtg gaaacaaccg aagaaattga cggaataatt aaaataaata
    39121 ttgatattgc atctgaatgt attcaaagaa agaaaattga ttacgacaaa gatggagaca
    39181 accattatga tacaatatcc gcatttatta aaagcatgag gggctctgac ccggatgcgg
    39241 cagtttattg gcttgcaaaa atgataaagg ccggagaaga cccgaagttc attgcaagaa
    39301 ggataattat ctgcgcttcg gaagacgttg gaaatgcaga ccccagagct ttggaagttg
    39361 ctgtgaatgc atttaaggca gttgaaatag taggctttcc cgaaggacgg ataattcttg
    39421 cccaggccgc aatatatatt gcatgtagtc caaagagcaa tgcctcaata attgcaatcg
    39481 acaaagcaat gaatgcagtt gaaaatgaat ctcatgaagt acctgctcat ttaaaagata
    39541 aacactacaa aggtgctgaa aagctaaaca ggggaataga atataaatat ccccatgatt
    39601 atgataaatt ttatgtaaaa caacaatata tgcctgaaac aataaatact aaattttatg
    39661 agcctaagga atcaggctac gaaagcagaa ttaagaaatt cttggaatat ttgaggcagt
    39721 aaggaggagc agcatgtaca aaatttttgg aaaaattacc gacccgaata tagaaaatat
    39781 tataagctct aagctcaatt ttgacaattt gaatgttgat caagtatacg atatatatgt
    39841 tgatttttac aacacggatt tggaagaagg catgctaaat gctgatattt cttatgaaat
    39901 taagaaggag cattatttgt ttataaaaaa aatcagagct ttattcgaaa acaatcaaat
    39961 tcgagtaaat gagttttatt tgatgggtac catatccgac ttgccggaaa atgaaataaa
    40021 tatttcgatt atgaaatcac agggtgataa aaagaaaaat gtagtgtggc cttgcaaaga
    40081 aatattttta tatgaaaatc agaaaaacaa gttggacgca ttacttatca gcaatcaaat
    40141 ttctgaagaa gactatgaat caaatttaga atttttgaaa gatgaactaa atatatatga
    40201 aaatgatgag gaacataaat atataaatta gaggaagggg gacaaagaaa tgtccccttt
    40261 ttgtaacctt ctttgctaat attaataaaa tagaggcgag gaggtatcct atggaagtct
    40321 atattattac ctttttgcac gggaattatg ctgttgccgc attcaacagg ctgcaaggat
    40381 ttggagttaa gaatatacat ttgattcaaa cccctttcag cataaagaat gagtgcgatt
    40441 tatgcataaa gacaaacgat agaaaaacat tggatattat tttaaatgaa tgcaggggta
    40501 gatatcccgt aagcaatgtt tatactcaaa taaacaaaaa tggttccttt gtatataaat
    40561 tattaccata aaatttataa gcaaaaaata ataaaaatat ccgctgatat ttttattatt
    40621 tttatttttt ttaatgttga caaaattact agggtattct ataatgaatt atacccgact
    40681 aaaacagtag ggattccatg aaaggagagt aaaaatgatt ttatccacaa agggcagata
    40741 tggacttaag gcggcatttg aactttcaaa aaattatgga ttaggacctg tttctctaaa
    40801 gaaaatatca gaaaagtatg gtatatcaga gaattactta gagcagctgt ttgcaaaatt
    40861 aaaaaaaagc ggttacatag atacggtcag aggacctcaa ggaggatatc ttctagcaaa
    40921 aaaacctgag gaaataactg ttggaatgat actcagaaca cttgagggtg aaataaaaac
    40981 ttctgattgt ttaggcaaag aagtatgctc cagagaatca atttgtgcta caagaattat
    41041 atttgagaag atagaaaaga gtattaacga tgttattgat aatataacct tagctgatat
    41101 gtacgaagag caaaaaaaca ttttaacgga gggaagaaat gagcaggaaa ctttatcttg
    41161 acaatgcagc aacaacaagg gtgaaaaaag aagtaattga tgaaatgaga caatattttg
    41221 atattttgta cggaaatcca tcaagtcaat tatatgagtt aggaagaaaa tcaaaagaag
    41281 ccattgaaaa ggcaagaaaa actttggcag actttttgaa tgcagaagaa aaagaaatat
    41341 attttactgc aagcggtacg gagtctgata actgggcttt aaagggagtt gcatttgcaa
    41401 atctgaacaa aggaaagaac catataatca caacaaaaat tgagcatcat gcaattcttc
    41461 atacttgtga atacttagaa aaattcggaa tagaaacaac ttatctggat gttgacagat
    41521 acgggcttat tgatatagaa cagctgaaag aatcaataag acctgaaaca atgcttattt
    41581 cagtaatgtt cgcaaataat gaaataggta ccatacagcc aatcgaagaa ataggaaaaa
    41641 ttgctaagga ccatggtata ttatttcacg tagatgctgt tcaagctttg ggaggtatca
    41701 gaatagatgt caataagctt catatagatt tgatgtcaat gtctgcacat aaaataggcg
    41761 gacctaaggg aataggcggt atgtatataa gaaaaggaac aagaattgat aactttattc
    41821 acggaggcgg tcaagagaga ggcagaagag ccggtaccga aggagtacag aatattgtag
    41881 gtttcgggaa agctgtcgaa attgctgcga gggattttga taagcatgtg gaaaggctga
    41941 cagttctaag aaacagactt attgacggaa ttaaaaacaa tattcccgat gtagtgctta
    42001 acgggcatcc gacacagaga cttcccaata atgtaaactt ctcatataaa tttgtggaag
    42061 gcgaatccat attattgctt ctggatatgg aaggaatatc agcttcaagc ggttccgcct
    42121 gcacttcagg ttcattagac ccttcacatg ttttgttggc aacggggctt gaccacggaa
    42181 cagcccacgg ctcaatccgt ttcaccttaa gtgaagaatt aacggaagag gatatagatt
    42241 atactgttga aacaatgaaa aaaataatta ccagattaag agcaatgtca ccgattaaaa
    42301 acgatgaaga tttgcataaa aactaattta cggagggaat tatgtattca gaaatagtaa
    42361 tggaccattt cagaaatcca agaaatgtag gtcatatgga gaaaccggac ggtgttggac
    42421 aggtaggtaa ccccaaatgc ggggatataa tgaaaatata tttaaagatt aaagatgata
    42481 taattgagga tgtaaaattt gaaacattcg gatgcggctc tgcgattgct tcatcaagta
    42541 ttgcaacaga gcttataaag ggaaagagca ttcatgaagc ggttgaactg acaaacaaag
    42601 ctgttgtaga agccttagga gggttgccgc ctgttaaggt gcattgctcg gtattggcag
    42661 agcaagctgt aaaatcagcc ctttatgatt atgcaaaaaa agcaaacaag gtaattaagg
    42721 gtttggaaaa ttataaacct gaggatgatg agatacactg tcaacactaa agatcccaca
    42781 agacagtgaa cgacttttgg gggacggttc tttttgttat catttgagcg aacatcagtc
    42841 gcttatctaa gaacaaaaag taccgtccct aaatgtttct tgacaatgag ggaaagaata
    42901 cattataatt tcataataaa gtacatattg cttatactat gaaacggagg aaataaagtg
    42961 gataaaattg atagattttc aattaataat attcgtaaag aatacttgaa cttttttaag
    43021 agcaagggac acttaaccga gcaaagtttt tctttgatac ctaaaaatga taagagctta
    43081 ttattgatag gagcaggtat ggctccgtta aaaaaatatt ttacgggagc tgaaattccg
    43141 cctgctaaga gaatggcgac ctgtcaaaag tgtgtgagga cgggtgatgt tgaaaatgta
    43201 ggattaacag cgagacattt gacttttttt gaaatgctcg gaaacttttc cttcggtgat
    43261 tatttcaaga atgaagcaat tgtatgggcg tgggagttat taaccgatgt tttaaaaatt
    43321 gataaggata gactttgggt tacagtatat ttggaggatg atgaagctga gaatatttgg
    43381 cataccaagg taggaatacc tatggaaaaa atagtgaggc ttggaaagga tgataatttt
    43441 tgggagttag aagtaggacc gagcggaccc tgctctgaaa tatattatga cacaggagaa
    43501 gatagaggct gcggttcaac tgactgtaaa ccgggatgtg actgcaacag gttcatagaa
    43561 atctggaatc ttgtatttac acaatttgac aaggacgaag aaggtgtata tcatcccctg
    43621 cctaatccga atatagatac aggaatggga cttgaaaggg tagcttgcgt gcttcaagac
    43681 aaacccacgg tgtttgatgt ggagccccta gtttttatag tcaataaagt ttcggaaatc
    43741 tgcaaaatac cttatggtca aaatctagaa agcgacgttg caatgaaagt aatctgcgac
    43801 cactcaagag cagcgacctt catgataggc gatggagtaa taccttcaaa tgaaggaaga
    43861 ggttatgtat taagacgatt aataagaaga gcaatccgca agggcaaatc attagggata
    43921 aatggcgaat ttttaactaa gactgcaaag gttgtaatag ataattggaa agaagtttat
    43981 ccggaattag gagaaaaaga aaaatacata ttaaaggttt taagtcttga agaagaaaga
    44041 tttaaaataa ccattgacca aggtcttgaa cttttaatga aagaaattca aaaactaaaa
    44101 gaaacaaata aaaaagagct ggatggaaat gtagcattca aactttatga cacctatggt
    44161 tttccttcag aattgacaga agaaatactt aaggaaaatg gttttagcct aaataaagag
    44221 cagtttaacg cagaattcga agagcacagg cagatggcaa gagaagcaag gaaaactacc
    44281 ggcgcttcag gttggaagga caatgatttt accttggata cggataaaac catatttaca
    44341 ggttacaata aattagaaga tatatccgaa gttattgcca ttgtagtggg ctctgagcag
    44401 gtaaatacaa taaatgaagg acagtccgga attatagtgc ttgataaaac tccattttat
    44461 gccgagggag gcggacagac cggtgattcg ggtgaattca ctgcagatga aatgctggca
    44521 aaggtaaccg atactcataa attcaataat gggatttatc tgcatgacac caatctggtt
    44581 aaagggagta tttccgtcgg agatacaata actgcaaaag taaatgatga ccaaaggaaa
    44641 aatactgcaa agaatcacac ctgcacacat atattgcaca aggttttgag ggaaatactg
    44701 ggagaccatg taaatcaagc aggctcatat gtttctgaag atagacttcg atttgactac
    44761 acacattttg aagctcttga taaaaaaaca ttaaaagaaa tcgaaaaaag agtaaatgaa
    44821 gcaatactat cagaccacac ggttaaaacg gatatcctca gtatggaaga agctaaaaag
    44881 tccggtgctg tagctttgtt tgatgaaaag tatgaagaga tggtaagagt tgtaagcgtg
    44941 ggagatttca gcaaggagtt atgcggtgga actcacattg accaaacagc taaaattggt
    45001 atgtttaaaa ttatctccga aggaagcata gcttcgggaa taagaaggat agaggctatt
    45061 acgggaagag cagccattga ttatatgcag gaattggata atttaatcga tgaattgtcg
    45121 atatccatga aaacaaccaa ggatgaattg ttaagcaaag caaatcttct gaaaaaggaa
    45181 gctaaagaaa aagataagga aattcaaagg ttaaacaatg aaatattaaa gagcaatata
    45241 aatgagattc ttgacaaata tgaagagatt aacggtatta aactttttgc cataaaattt
    45301 aaagataagg atgcaaatac cttaagagaa attgccgata aaattagaga taagaatgag
    45361 agctgtgcta taatattagc ttcagattta ggggataaag tcttatttgt atcggctgta
    45421 acaaaggatt tggcaaaagg cggaatacat gcaggaaata tggttaaaga ggcagctgtg
    45481 attgcaggcg gaggcggagg cggaagacct gactttgcgc aagcaggagg gaaaaacccc
    45541 gagaaaatag acgaagcaat taatgcggta agaaaacaaa tagcctctat atgacaaggg
    45601 gacgggggta ctgtcatcat tttgagtacc attagcacga agaatttcat gttgttttat
    45661 atcataatat agtaattgat tttatttaaa aactatagta gaatataagt aggtgtaaag
    45721 aggtggtttt tatggatagt aatgtaaata atacggcaaa atttgattac agtatgggtt
    45781 cattgaatga agctcgggaa ataatttcac aggtctacaa gtcgcttaaa gaaaagggat
    45841 acaatcctaa caaccagctt atcggatata tattatcggg ggaccctact tatattacaa
    45901 atcataataa tgcaagaagt ttaatacgaa aaatagaaag agacgaactg ttggaagaaa
    45961 ttttaagtgt ttacttaaat gtaatcgaca tttagaggag gggggacaca ttttgaatgt
    46021 ccctgtacta acttatatct tgagcagtat ataaatatac tgcttttgta tgccctaaca
    46081 agtttcttcc gataaaaata taaatatgaa atacataaat ttatttgaaa agaaaaatgt
    46141 atcaaggctg tgttacggaa cactatcttt aagcagtctt caaaatctcg atactgttga
    46201 aaataaagta aggctgctta attatgcata cgaaaaagga ataaattttt ttgacacagc
    46261 ccagctttat gataactata atatacttaa agaattcata aaacataaag acagggataa
    46321 actagttata gcttcaaaaa gttatgcata tgacaaaaat actgcggaat atagtatcaa
    46381 taaagcattg aaagaaatga acactgatta tttggatatt ttcatgcttc atgaacagga
    46441 taacgggaat aattttttgg gtcattataa ggcagttgag cgtttcatga aatataagga
    46501 gcagggaata atcaagtact ttggaatttc aactcacagg gtggaggcag taaaagattc
    46561 acttaaattt aaggaaatag aaattatttt tcctttgatc aattataaag gtatcggaat
    46621 tcaagacgga acttttgaag atatgactga agccttgaag gaggcaaagg aaaataataa
    46681 gtttattatg gcaatgaaaa tatatggcgg aggccattta ataaaggaag cagaaaaagc
    46741 atttaagtat attgacagtt taaattatgt ggattccata gcaataggta tgagcactgt
    46801 tgaagaaata gatgcaaata tctcttattt ggaaaataat gagttaaata ttaatataaa
    46861 aaacaaaata aaaaatcaaa atcgcactct cgtaatagaa gagtggtgtg ctggatgcgg
    46921 aaaatgcgta aaaaaatgca aacaaaatgc acttgcaata gttgacggca aatgtgcggt
    46981 agatatggaa aagtgtgttt tatgcagtta ttgcgccggt gaatgcgcag gtttttatat
    47041 caaaattgtg tgaggtttct atggacagat tgatgggatt ggatgtggga gataaaacaa
    47101 tcggagtagc tgtcagtgat ttattgatga ttactgcaca aggagtaact acaataaaaa
    47161 gaacgaatat taaaaatgat ttaaacgaac ttaaaaagct catcgatgaa tatcaggtta
    47221 ctaaaatagt ggcaggcatc ccaaaaaggt tagacggtac aattggaata cagggagaaa
    47281 aggtgcttga atttttggaa aagatgaaaa agcatataga tttacccgtt gagtttgaag
    47341 atgaaaggtt taccacaacc atgtccgaaa gaatgttaat agaagctgat gttaaaagaa
    47401 aaaaaaggaa agaagttatc gacaaattag ctgcggtaca aattctgtca agttatatgc
    47461 agcgtacaaa ataatcaaag gagatattat ggataatatt attgaactga taaacgacga
    47521 aggaaagcta gaaaagttag tcgtagaagc cacttttcac attgatgata ttttgtatgc
    47581 agtacttcat aaaataaact cggatgaagg tatggtttat tatgtggaag aaatggaaga
    47641 cggaagtata gtgctggtta aagttgaaga cgaggaagaa attaaggaag tgcaggaaat
    47701 atatgaagga atagctgatg atttaattta agctgtgtcc tggggggtag ataaatggca
    47761 gattatttaa gaactttgct tgataaaaat ggttacaaaa caactgttca acgaaaaatt
    47821 ttatatgaag ttctttctga taacaaagat aagcatttat ctacggaaga aatttacgac
    47881 ctaataaaaa acaggtaccc caatattggc atagcaacca tttacagaac ccttctcttg
    47941 tttgaggata taggacttgt atataagcac aacttcgatg acgggttttc cagatatgaa
    48001 atattatcac aaaacagtaa tgaaattcat cagcaccatc atttattgtg caaacaatgc
    48061 ggcaaaatca tagaggttaa agaagacctt atgaacacat tggaagaaat aatcgaaaaa
    48121 caatacaatt ttgaaatatt aaatcatgtg gtcaaattca ccggaatctg tgcacaatgc
    48181 agagataagg agaataaaga tggcagaaaa gaaacataaa ttaaaagtaa taccattggg
    48241 gggactcggt gaaatcggaa aaaacataac cgttattgaa tatcataatg atattatcgt
    48301 aatagacagc ggaatggctt ttccgggaga tgaaatgctt ggtattgaca tagtgatacc
    48361 ggatattact tatttaataa agaacaaaga aaaagtaaag ggtattatat taactcatgg
    48421 tcatgaagac catatcggct caatacctta ctttttaaaa aagctaaata taccgattta
    48481 cggaacaaaa cttaccttag gtcttgttga aaataagctt ttagaacata aaatagaaga
    48541 tgcatcctta aatcccatta atgctgggga tacattaaaa ttaggatgtt ttaaaattac
    48601 gcctataaga gttaatcaca gtatacctga ttcagtgggc tttgccatag atacacctat
    48661 cggtacaata gtccatacgg gagattttaa aattgattat acacccatca atgaagatgt
    48721 tatcgattta ggtaagtttg cggatattgg aagaaaaggc gttcttcttg ccttttctga
    48781 cagcactaat gttgaaaggc ctggcttcac ggaatcagag aaaacaattg gtcagaaatt
    48841 tatggatata tttaaaaact gtgattcccg aataattgtt gcgtctttcg cttcaaatat
    48901 tcataggctg cagcaggtta tcgacgcagc ggtcgttaac aaaagaaagg ttgcaatatc
    48961 aggtagaagc ataataaatg caattaaagt ttctctggaa ttaggatatc tgaatgctcc
    49021 acagggaaca ataataaatg taaacgagct aaacagcctt aaggatgatg agattgttat
    49081 attgactaca ggaagccagg gagagcctat gtctgccttg acaagaatgg ctaacaatga
    49141 ccataaaaaa attcaaataa aagaaggaga cttggttata atttcagcca atccaatacc
    49201 cggaaatgaa ataacagttt caagagtaat caatatgctc tatgaaaaag gcgcaaaggt
    49261 gctttatgat gcactgacac aagtgcatgt atcaggccac gccagaagag atgaattgaa
    49321 gcttatgctt actctgttaa aaccaaagtt tttcatacca gttcacggcg aatacagaca
    49381 tttggtgcag catgcaaagc tggcgaatga aataggcatt ccaattaata atactgtaat
    49441 aggcagcaat ggtgacgtaa ttgagcttac ttccaagtca atagttaaaa acaacactgt
    49501 tacatccgga aatattcttg tagacggctt aggagtgggg gatgtcggga atatagtcct
    49561 tcgagacaga agacttcttt cagaaaatgg tttgataata gttgtactat ctaccgatag
    49621 aagtacggga aaaattttag ccggtcctga aatagtatcg agaggattca tatatgtacg
    49681 cgagaatata gatttaatag aagaatcaaa aacagtggta agaaaagcat tggaaaagtg
    49741 tgaagataca aatgttaaag aatggaacaa tattaaaatg gtaataaagg attccttagg
    49801 cagctttatt tatgaaaaaa taaaaagaac tccaatgata cttcccatta tagtagaagt
    49861 tgatttggaa gtataaattt agggacacaa aataaatagg gacttaacgt ccctattttt
    49921 gttaagaaca ttggtgccag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50041 ntggtgccag gcaccaggaa atactgaacg cagtgaagaa tctcataacc atttatagtg
    50101 taatagattc gaactatatc atttgtcctt tcatataata aataaaatac atatgggagg
    50161 taacaaacaa attatgatca ataatgaaac tatagaagcc gaacgtacaa gggtatgcct
    50221 gacaatcgga aggattgcac ctgattttac agccgttact acagagggac caataaccct
    50281 gtctcaatat agagggaagt gggttttgtt tttcagccat ccgggagatt tcactcctgt
    50341 ttgcacatca gaatttattg catttgctca acttcaacct gaatttgaaa aaagaaatgt
    50401 tcagctctta ggaataagca ttgattcaaa tcctgcacac cttggctggc tctacgatat
    50461 attccaaatg acaggtatag ttatgccctt tcctcttatt gccgacaggg atgcaagtat
    50521 agcagcgctt tacggcatgg ttagtcctga cagaatctat gagcaaagtg ttagagatgt
    50581 ttttattata gacccaaatc aaagaataag gtcaattctc atctatcctg caactaacgg
    50641 cagaaacatg tatgaattgt taagattaat tgatgcactg caagtgtcaa caacatatgg
    50701 tgtaagcacc cctgcaaact ggatgccggg ggacccggta attgttcctg ttgcaagcac
    50761 cctcgatgag gcaattgaaa gaggcatgga tcccgaaagt ttagaactga actgtcttgc
    50821 atggtggtat tgttttacgg atttatctga actgacacct ttaccaaatc cataatatgt
    50881 cgataaaatc cttcactaag ttcaggaaga catacaataa gtccctattt attttttagt
    50941 tgaaaaaatg gggtaaaaaa acaaccggtg agcttttagg tcaccggtta ttaatgctta
    51001 ttatcttttg atgttgtgaa agtttactcc taagattggt ctgaactggg atggtttgtt
    51061 gtttacaata cttttatctt ccattctttg atttgacctt tttgaagtca aatttagatt
    51121 tggggttatt ctaccgttga tagcactggt taaattgtat tgaccatccc ttctaagttt
    51181 aatcacatct aaattaccaa ctttggataa atcgggagtg ctaattttgt tcaatctgtc
    51241 cattagtatc aaccctctct taatcaaaag aaataagcat tattatatta accagtcaaa
    51301 taattattat actattaatt ttttaaatta ttgtaattat tagtaaaagg ttgtataatt
    51361 taatggattt agaaattatc tactggagga attatgaaaa agataattgc actactgtgt
    51421 gttttgtcaa taatagcagc ggcgtatctt tatttaaata attatattga agaaaattta
    51481 gaagctgttg atttggggga tgattctccg attgttgttg aaattcccgc aggaagcaca
    51541 actaatgaca ttgctgaaat tttgtttgaa aataatttaa tttcaaatat acgcgtattt
    51601 aagtattatg ctgaaaagac agggtcagat gctaaattaa aggccggaac ctatattctt
    51661 tcaaaaagca tgaatgctga tgaacttttg gaatccctta taaaaggagg ctcatcaggc
    51721 aatacttaca atatcacagt gatagaaggt ttgactaatg aagatatggc tcagtccttg
    51781 tcggagcaga ccgctttaaa ttatgacaga tttattgaat tgatggaatc tccggaaatt
    51841 ttcaatcaag attttgaatt cttaaaagat tcatctgtta caaatttgca gggataccta
    51901 atgcctgata cttacaacat ttatgttgat tcaacggaag aagatattat tagagttctt
    51961 ttaaaacaat ttgataaatt ttatattgat gaaattaaac caaggataga taaaacaaac
    52021 cttacatttg aagaggttat aaatttagca agcatagttg aaaaggaagc tcttttggat
    52081 gaagaaagag atgaagttgc aaaagttttt ttaaaccgtc ttagtattaa catgaagctt
    52141 caatcctgtg cgaccgttaa ctatgcaaac ggagagtgga aggaaagact tacccttgag
    52201 gatattgaaa ttgattcacc atacaatact tatattgtgg aaggactgcc tccaactccc
    52261 ataaattcac ccggcagagt atcaataata gctgtattgg agcctgcaga tgttgattat
    52321 ttatactttg ttgcaaaagg agacggctct cattatttct caaacacata tgatgagcat
    52381 atagatgcgg ctgaagagta tttgaattga ggaggtatac tttgaataat attaactcat
    52441 cttatataaa tgaatatata aacagcctta ttattgaaga tagcgaaaaa ttaaataaat
    52501 tcagagatta ttgcgaaaaa agaagtctgc ctatcatcca taaggaggta ggccaattta
    52561 ttaaattagt tataaatctt ttaaatgcaa aaagtataat tgaaataggt accaatgtag
    52621 gttattcttc aatatttatg agcaaggtta tgaaagatga gggtaaggtt gtcacattgg
    52681 agagaagtga aaaattttat aatgaagcat taaaaaatat tactgatttt ggacttgaaa
    52741 aaaaaataga ggtttatttt ggagatgctg ttgatatatt ggaccaagta gaagggactt
    52801 ttgatatggc ttttatagat gcagccaaaa gctattacag ggtatttttt gacaaatgct
    52861 taaaaatgat gaagcccgga gggattattc tttcggacaa tgtattatat caaggaatga
    52921 ttgcatcgga tgaattggta gtaagaagaa agaaaacatt agtaagaaat ttaagaaatt
    52981 atttagaata tatatctcat gacgagagat ttgtaacttc ggtacttccc cttggggatg
    53041 gattggcagt tacgctgatt aaatagggat ggaacacacc gaaaactttg ttttttaaag
    53101 aatgtcgctt taattgaaat aaccagatag agaaagagga gaaagaatgt tgccaaaatt
    53161 attagcacct gccggaaatt tagacaagtt aataatggcc gtacaatacg gagccgatga
    53221 agtttatttt gcgggaaaat caatgggaat gagagcaggg agcaaaaact tcactaaaga
    53281 cgatttaaaa cagggagtgg agtattgtca taaatatgga agaaaagcaa atattaccgt
    53341 taatataatt cctcataatg atgatttaaa gggtatagaa ggatatttga aatatttgca
    53401 taaaatcggt acagatgcac tgattgtagc tgacccggga gttgtggaaa ttgtaaagca
    53461 aacaattccg gatatgaaaa ttcatttaag cacccaggca aatacaacaa actatcacac
    53521 agcaaacttc tggtataaac agggagtaaa cagaattgtg ctagccaggg agctgacatt
    53581 tgaggaaatt gaaactatta taaaaaacac accggaagat ttagaatttg agacatttgt
    53641 acatggagcc atgtgcatat cctattcagg cagatgcctc ttatcaagct ttatgaccgg
    53701 caggttttca aataagggtg actgcgccca atcctgcaga tggaaataca acttggttga
    53761 agagaaaaga cccggggagt attatcccat agaagaaacc ggtgaaggta cttttattat
    53821 gaattccaag gatttatgca tgatagagca tttgcagaaa ttcaaggagc tgggaataaa
    53881 agcgttaaaa attgaaggta gaaacaaaag tgaatactat accgctttaa ccgtccgctc
    53941 ttacagaaat gcattggatg agctgaaata tccggaaagt gaaagaaata ccggatattg
    54001 gaaggaagaa cttgaaaaaa catcgcacag ggatttttcc tatggatttt tcttcggtaa
    54061 tccctacaag gatggacagt tgtataccca ttcaagctat ataaggacat atgatgtggt
    54121 tgggataata aggtcgtatg acgagtcatc aaagattgca acagtggagc agagaaataa
    54181 attttcagaa ggcgatattt tggaatgctt tggtcctaaa ggaaaacact ttgaattaaa
    54241 agcagaaaac ttaacagatg aaaataataa taaaatagat tcagcacccc atccgcagca
    54301 aacagtaaaa atgtatattg aaaaaaatgt agagccatac tttattttga gaaaaaaact
    54361 ggaagaataa aattaaataa atgacaaggg aacgggggca ctgtcatctg aatgatgaca
    54421 gcacccccgt ccccttgtca tcaatttcca acgttgttca ccaaggcgtc atggagtttt
    54481 tttagagcct tcttttcgat tcttgataca taggagcgtg atattccaag aagtttggca
    54541 atttccctct gagttttatg ctctttgttt cttattccgt aacgcatttc aataataaat
    54601 ctttctcttt tttgtaatgt tttatccaat atttcatcca gggcttttat tttttcttct
    54661 ttatccaatc tgcctactat ttcttctcct tcatatttta ttatatcgat gagataaata
    54721 ttattgcctt ccttgtctgt tcctaacggt ccttgaagag atacttcttt ttttagtttt
    54781 ttttcacttc ttatagtcat aagtacctca ttgtcgatgc atttggcagc ataggtggca
    54841 agtcttattc cttttttttc atcaaaagtt gaaacagctt taattagacc gattgttcct
    54901 atggatatta aatcctcttg gtctattcct actgtcttat atttttttat aacatgtgcc
    54961 acaagacgaa gatttctttc tataagaata tttcttgctt ctatatcgcc tttatttgct
    55021 ctttctataa ggcatgcttc ttcttccttg gttaaaggat gtggaaatga gcttccgtta
    55081 gttatgtaac caaaaataaa tattgatagc ggtaataaga tttgtagtag ctcttgtatc
    55141 aaaatcaccc ccaaagttat acattatatg caagatatta aaaaaactgt atgtacactt
    55201 taaaaacttt aaataaaagt aatatttaac agcatttctg ccattaaata ttacacttgt
    55261 ttttcttaca tttcctcatt cataggcggg acagttccat tttctcctcc tccaccgtcc
    55321 atcgggcagt tcatacgtct catcagttct cttaattgct cgcagttaat agggggcatt
    55381 ccgtttggca tcccgggcat cataggatac atattgccat catcaggcgg cattccgggc
    55441 atctgaggca ttccgtttgg cattccgggc atctgaggca ttccgggcat ttgcggcatc
    55501 ccgggcatac ctggagtggg ctgcataccg ggcatttgcg gcatcccggg cattccggga
    55561 gtcggttgca taccgggcat ttgcggcatc ccgggcattc cgggagtcgg ctgcataccg
    55621 ggcatttgcg gcattccggg cattccggga gtcggctgca taccgggcat catgggtatt
    55681 tcgggttgtg tcaaccatgg cggtattaaa ctaggaatcc cttgttgcgg aaacctcggc
    55741 agagcaggca tatctccgtc aatttctaaa ggcatttccg tttccggaat gaatggcctt
    55801 tggagcatat cgtcaggcat ccccggcatt ctgggcattg tgtttgcaag atgcattcta
    55861 ttagaatttt tgtgctcatt tatgcccatt ggattatagt tatggggcag tgagtcgtag
    55921 tggggcatat tatttctata attattcata ctatcctcca ttaaataaat ttctcagtat
    55981 taatatacgt ttgaaaaaaa tattagtgaa ttaaaaataa agaattcttt actattttaa
    56041 taaattcata taaactatta gtcaaaggaa acagggttga ggagaatatc tttactatgt
    56101 tacaataaaa gacgggcgga aaaagcaatc taaaatattg atgaaaatta tgtataatta
    56161 tggtattata taacaaggag atgaaagtat gataaatata ttacttgtga cacatggaga
    56221 gtttggtaaa gaattattga aaagctctga aatgattata ggagaagtag aaaatgccaa
    56281 aagtatatca tttaaccatg gtgacagttt tgaaacattg ttaaaaaagg ttgaagaaaa
    56341 tattaaaaat atatctgatg atgaacttat cgtatttacg gatatgtacg gcggaagtcc
    56401 ttacaatgca gtaaatcgtg caatgaaaaa cagaaatttt tatcatataa caggtataaa
    56461 cttccccttg tttatcgata ttgctgttgc tcgcgataca tacagcttag aggaaattgc
    56521 agaaaaaata attaaaaacg ggaagaagag catagtgttt gtcaatgaaa agttcttatc
    56581 agactgaggt agaaaatgaa aaatgtattc gttagaattg atgatagatt gttgcacgga
    56641 caggttgttg tatcttggat accttacctt agagcaaaag aggttgtaat tgcggatgat
    56701 gaatatgcaa atgatgaatt tatgagtgaa ctcataaaga gttcagggcc tgaaggtgtt
    56761 atagttcatg tgaagacaat cgatgaaacg gctgcttatt taagcgatga caatcaaaac
    56821 aagatactgg tacttctcag gagtattgaa ggtataaaaa aattaataaa aaaaacaaaa
    56881 ataagcagta taaacatagg tggagtggga gcagcccagg gaagaaaaag atattataat
    56941 tccattcatt taagtgacga tgaattaaat atattaaatg acatagcaaa tgacaatata
    57001 tatgttgaaa taagaatcct tccaaaggat aaggctatca taataaaagg taattgacat
    57061 gactttttct gattattgtt tacttagcat tttttatttt tgggcaagca gtactgcatt
    57121 ttccgtaggt atcggttatt atacaatata caggcctata atggcaggaa tggctgcagg
    57181 tttaattctt gctgatataa aaacaggcat gttggcaggc gcagtagtta atattatttt
    57241 tattgacttt atttctaccg ggggttcatt gaaaggagat caatgtctaa ctgctatatt
    57301 ggcggcagca gcttcaataa tatttaagct taatcctctt gaagcagcag cagcagcttt
    57361 tcccttcggg tttttaggga tattgatatg gaaatacagg ttgaaaataa ataatatttt
    57421 tgtacatatt tacgagaaga aatataaaga gaacaaagct cccgacataa gtttgtacaa
    57481 cggacttttt ccgcagctgc tgttatttat gatgagtttg gcggttatta taacggcatt
    57541 ctatttaatg tttttattca ataaattaat acattttaac agtattagcg tattgcttta
    57601 ttatataggg ataatactgg ttattttttc cgtttcaaat gttttataca aaattaagat
    57661 taaatataat tatgcaattt ttatttctgt ttttttggca gccttagtat tagatatgag
    57721 agcatatata ctgtttattc ttatagtatt gagcctttta tatttgtcag agaagaaagt
    57781 gagcattgtc aggaatagtt ttaacggtaa attaaaaaga ggagacttgg tttattcatg
    57841 gtttatatgg atgaattatt ctcattcatg ctacagctat gaaagactta tgggattagc
    57901 gtttgcccac agtatgaaaa atataattag aaaattatat gcagatgaac atgaaatagc
    57961 agaggcaata cacaatcaca ctgaattttt taatactgag cctaatatgg gtactccaat
    58021 acatggatat ataatttcat tggaggaaga aagaatgtta aacaatcaaa gttttgagga
    58081 tatttcctac ataaaaaaag gtatgatggg aatatcggca ggattggggg attctttcac
    58141 tcaggcaatt ttggcaccgc ttttcatatc catgtctgtt atgctttgtt tagacggtag
    58201 tttttatttg gcgtttatac ctgtaattat cctgtcagtc tttatacttt ttatttctta
    58261 caacggtttt atgaatggtt actttcaggg aagagaaagt atgcttcaaa gaataaaaga
    58321 tgttaaacaa agcaagataa aattatattt tccttatatt ttttcgggaa tattaggatt
    58381 ttcaataagc aaattgcttt ttaacaacat aagaccctat gaagatatat ttacattgag
    58441 tattatttta tttgcagctg ttattatgtt tattagaaaa aggggagagc aatgaaaaaa
    58501 aaagttggaa taatgggcgg ctcttttgat cctatacata tgggacactt agttgcagca
    58561 aatgaagcat taaatatata taaattagat gaaattattt ttattccgac aggtgacccg
    58621 ccgcataaag attatcctaa tgctgaatca tttcacagat tgttaatggt taatttggca
    58681 gtgctgacta atgacaaatt cagtgtatcg gatattgaaa ttaaaaaccc gagcaaaagt
    58741 tataccttga gtactttaag ggaattacac aagctgtata aaaacacaga attttatttt
    58801 ataaccggta ctgatgctgt tttaaatctt ccaagctggc accagcctga agaagttcta
    58861 aagctttgca ggtttattgc agctacaaga ccggggatta gtattgagga agtaaacaca
    58921 aagataaatg aaataaaaag aatgtttggc ggaaatatag aaatattaca ggtccccatg
    58981 cttcaaatat cttcttcaga tataagaaat aggatcaaga tgggtgtttc cataaaatat
    59041 ctgttacctg attctgtgga gcaatatata attaagaaca gcttgtataa aaacgggcaa
    59101 gaaaagtgaa ggatatcaca ttatttagac agcggagagt aaaatgcata ttgaagaaat
    59161 gaaaaaagaa ttaaaaattg aattaggtga aaagctgtac cgccacagca tcggtacaat
    59221 ggaagaagct gaaaaacttg cttcggttta tggttgtgat gtttcaaagg caataattgc
    59281 aggattattg catgactgcg gaaaaagaat atgcagcgac aatttgaaac atgcaaaaaa
    59341 aagtgcagag cttgccgggg taaagtatgg agtaaaagac aatgatatac tcaatgcaat
    59401 aatgtttcat acaacgggaa gagtaaatat gtctcttttg gaaaaaataa tatatattgc
    59461 agataaaatt gagccaaaca ggaattatga aggtgttgaa gaaattagaa ataaagcata
    59521 tactagactt gatgaagcaa taataaaatc tttggaaaac acaattgaat acgttaaaaa
    59581 caggaatttg gttcttgata tggaatcagt aaatacactg aattatttaa aggagggtaa
    59641 atgatagaag ttcaaaaaaa tattgaaact attttaaaag cttgcgataa taaaaaagcc
    59701 tataactttg ttgttttaaa tgtgtctaaa accagctcga ttacggatta cttcatcatt
    59761 tgcagcggga acaacgagaa acagacagtt gcaattgctg aagaagtgtt ggaaaaagga
    59821 gcaaaggaag gaatagattt tttctataag gaaggttttg aaaccggaag atggatttta
    59881 ttagaaactg atgacattat tgttcatatc tttcataagg atgaaagggc aatttatgac
    59941 ctggaatcat tgtggtatga taacaattca ttggatgttg aacaatatgg aataaaaaat
    60001 tggaaatagg aggtaattat gaatagtgtt attcatacta acaatgcacc ggcagcagta
    60061 ggcccttatt ctcaaggaat aaaagccggc aacacaatat atgtttccgg acagcttgca
    60121 ataaatccgg caacagggga gctgtacaag ggcggtgaca ttaaagaaga aacaagaatg
    60181 tccttaatga atgtgaaagc tattttggaa gaagcaggag caacattgaa ggatgttgtt
    60241 aaatgcacgg tatttatcaa ggatatgaat caattcggac taattaatga agcttatgct
    60301 gaagtattcc aagatacaaa accggcaaga gtttgtgtag aagtggccag acttcctaag
    60361 gacggaaatg tagaaattga tgcaatagca gttgtttaaa ggtgtgggga cacacctctt
    60421 ttttttatgg atagtctctg agggatagga ggaatgtccc caaattgggg ggacacacct
    60481 tcatggtgag tcagccgttg gggtaacgcc atgaaggaat gtccccgtac ttgtgtgggg
    60541 acacaccttt ttttgtcatg gatagtcttt atgggatagg agatgtcccc aaattagggt
    60601 cgttgggatc cgtgaaggaa tgtatctgta ttaaaaaaag ttgcaggaaa ctgcaacttt
    60661 tagtaattat tgtatctgct tcgtcttttc tttctgttga tacgaattct tatatcgttg
    60721 taaattttta aaagcaatat tgaaaccgcg gcaaataata taagaaaacc aataaacttg
    60781 acaattgaaa gtaaaatgtt cccttggcct tcctgtgcag tacttttaac cgatattgga
    60841 gatattaaat tcacggcacc tataacttta ttgtcaaggg tatattcaat tttccctata
    60901 acactgtttt tttcaatagg cagagttata tcgtttatat atacattcga tttaatatca
    60961 tcaagtgtgt cttttttgat aagagcagtc aaatcacttt cagtaatcaa agatatttcc
    61021 ttagaatctc cgctgggaat ttttatattt tttataaagg tatttttgcc aaccaatgtg
    61081 gcagattcat attcttcaaa cccataatta aaaagattat gagcatcttg atacatttca
    61141 agagaaatcc ccttcattgt aactgcaata agttctgtcc cgtctttttt tgctgttgcg
    61201 actaaacagt tgccggcttg gggagtgtag cctgtctttg ctcccgtggc atattcatag
    61261 taagggctta tatatgcacc atctacataa atttgattgt aacttgtatt atatattaat
    61321 ttattcaagg ttgtaaaata tcttggctca tcctttttat tggttgtttg tatttcatac
    61381 ttcgttgttg atacaatttt tctgaaagtg tcattttcca ttgtatactt ggttattaaa
    61441 gctaagtcgg ctgcggttga gtaatgattt gtatcgtgaa gcccgtgagg atttgtaaaa
    61501 ttagtattat atgctcctaa ttcttttgct ttttgattca tcatttttac aaaattctcc
    61561 aaacttcctc catagtgctt tgcaattaca gaagctgcat catttgccga aggcagcatc
    61621 aatgcataca gtaaatcttt taatgtcaga a
//
LOCUS       DAOPUE010000002        52537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_583_length_52537_cov_9.778591, whole genome shotgun sequence.
ACCESSION   DAOPUE010000002 DAOPUE010000000
VERSION     DAOPUE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 52537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52537
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_583_length_52537_cov_9.778591"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..800
                     /gene="cmk"
                     /locus_tag="PKH42_00335"
     CDS             150..800
                     /gene="cmk"
                     /locus_tag="PKH42_00335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOA18901.1"
                     /translation="MIIAIDGPSGAGKSTVARLLSKKLGYEYIDTGAMYRALAYKAYK
                     QNIDINEANIAELLETTNITYNDNKVFLDGENVENLIRNEEISTAASKISSLKIVREK
                     MVEIQRKIAKNKNVVLEGRDIGTIVFPDAEHKFFITASLEERAKRRYEQLKLNNIKAD
                     YTNVINDMIKRDENDSTRKFSPLKPAEDAILIDTTNMDLNEVTKTIAQYIGGSNVL"
     gene            790..1386
                     /locus_tag="PKH42_00340"
     CDS             790..1386
                     /locus_tag="PKH42_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOA18902.1"
                     /translation="MFYKIAVIVLKVLVFIIFDLEVYNRENLDNIDGGLIICGNHRSM
                     IDPVILAVSTKRQIHFMGKKELFENKFLSFLFRSLGAFPVDRKGVSLSAIKNSLNVLN
                     KGGILGIYPEGTRIKTGFDEKNAKSGIALIANKAKVGIMPVYLDGPYKFRGKLRLYIG
                     EEKDYFKNYSGRLNSEDYTEIGIEILKDIYRLKNEGMK"
     gene            1383..3797
                     /locus_tag="PKH42_00345"
     CDS             1383..3797
                     /locus_tag="PKH42_00345"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOA18903.1"
                     /translation="MKIIVSEYNGFCGGVKAAVLKADKILTDEKKLYCYGEIIHNKDV
                     VEKLKNKGMVIVDDIPENKDAKFLIRSHGVGKNIYDRLEEKNIEVIDATCVKVKKIHK
                     IVEEFKNKDYNIIIVGNENHPEVQGISGWCNYEAVIIKSPEELKNIIEPTKKYCLVCQ
                     TTFNLNTFEEILKTIEINNYSNIIVHNTICDATKKRQEACIALASKCDIMLIVGGKNS
                     SNTKKLYELSKELCENTFLIQNYKEIPYNYIDKNSIVGVSAGASAPDWIIEEVINMLE
                     NLNNNMNEMNEQAEHADLAEQSEPAELSDKKEQTELVEQSEETEKTETVEQTDKAEKV
                     NSMHELLENYGGVVNLGIGEKVKGTVIYVSPDAVSVNINYKSDGIITREDFSLEEVSD
                     LTEVVKEGDEIDAIVVKISDQDGNVVLTRKPLEEQKIWDDLEQMRIRGEIVEIPIIEA
                     SQHGIYGKLKGIKGFIPRNQISIKRNVDPSEYVGKNLKVKILETKNKKGRRQLVLTSR
                     AVEKFEKEIKDNEVWETIQEGEIYEGTVKNLQDYGAFVEINGIDGLLHINEIAWTRIK
                     HPSEVLKPGDKINVKVKNVDKENKRLSLSYKATVKSPWAKFLDKYQKGDIVTGVVTRI
                     VDFGAFVKVDDIECLLHIKDLDWARTEKVTDILQEGQEVTAKILSITRKDRKVGLGLK
                     QLTEHPFDLYAKTLNKDDIIQVEVTRILLDGIHVNANGNIDCFIPIAKVSTEKLRTPA
                     QVVKVGEIVDAKVQSIDMKGKNLNLTLILDDKSEETVENKSYQSEDTNFTIGESIGDA
                     LDDLLK"
     gene            3852..4892
                     /locus_tag="PKH42_00350"
     CDS             3852..4892
                     /locus_tag="PKH42_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOA18904.1"
                     /translation="MLKEFLFDICSNQFVSGFEHINGDILIKYFKPYTDSFEKDRLGS
                     YIFKSSGSKDTKIMLAAHIDEIGLMITGILDGGFLSFITVGGFNPASLIAQDVTIFGK
                     EKIFGVIGIKPPHLTSEEERKKPLKTRELYIDTGYTKEKLEKLITIGDIATINREPLS
                     LQGSVISAKAFDDRACVAVMLETAKELKKISHKSNVYFTATAQEETGLRGAKTSSYKI
                     APDIGIVLDVGFGKTPDMPSDSALLGKGPIVAYGGRLNTKLTKKFVEICKKYNYPMQY
                     EAIPVRTGTDTDALQISRDGIPCILLSIPLRYMHTSVETIDLKDLEITGKAIARFINE
                     LDNSDLEEILCF"
     gene            4883..5896
                     /locus_tag="PKH42_00355"
     CDS             4883..5896
                     /locus_tag="PKH42_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOA18905.1"
                     /translation="MFLNELTKLSGVSGCEFEVRNYIKSKLSEMGCQFYVDKLGNIIA
                     HNKGKKNKTIMVSAHMDEVGLIVTNIDSDGFIKFKAVGGIDQRVLNSKVVLIGENNIP
                     GVIGSKAVHLMTEKEKGVSLPIDKLYIDIGTSTKEETEKLVNIGDYAAFKSDYVEFGD
                     NMIKAKALDNRVGCSIILEILKEKLDVDFYGVFTVMEEVGLRGAETAAYQLEPDLAIV
                     LEGTVCADMPEVEEHENSTLIGKGAVLSLMDNATVYDIDLVRRVAKIAEDNNIAYQYR
                     RSTSGANDAGVIHKTKKGAKVVSISVPCRYIHSSVSVASKNDLMSALKLTKQVIIENQ
                     KGE"
     gene            5898..6881
                     /locus_tag="PKH42_00360"
     CDS             5898..6881
                     /locus_tag="PKH42_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOA18906.1"
                     /translation="MFNSELMKRLSDCFGPSGREKRIREMIMDEIKDFADEITIDPLG
                     NLIARKKGTGKKILFSAHMDQIGLIITHVDEKGLLRFANVGGLNAKELLGLRMVFDNG
                     LEGVICQEELKDNEKLSTSKLFLDVASTDKEFIAKNFRVGDMCVFKSEYYETEDCVIC
                     KAADDRIGCYVLIEALKNHPETNNDVYYVFSVQEEVGCRGAKTAGYNINPDLAIAVDV
                     TATGDTPDGIKMDVKLGNGAAIKLMDKSIITHTEVKDLLTDIAEKNSIKYQYEILEFG
                     GTDAGPIHTSRDGVPSGVISIPTRNLHTSGEIFNKFDVEECIKLVIGTAAK"
     gene            6985..7905
                     /locus_tag="PKH42_00365"
     CDS             6985..7905
                     /locus_tag="PKH42_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOA18907.1"
                     /translation="MKVGIPKGLMYCKYHPFLVTFFSELGAEIIVSDDTNKNILDAGV
                     KYCVDDACLPVKIFHGHVDSIKDKCDVLVIPRIMQLWKNEYICPKFCGLPEMVINSFD
                     KLPVTITEPIYATTKKKLYNWAKSSGKFLRKNNYEIKRAFINALNAQKAHEIGIYDSG
                     YEINIALTGHPYNIYDSFLNMNLVKKINALGMGIITAEFTGEDILQKESENLYKRPFW
                     TFVRENYGFAVNAAKEKNVDGIIYISSFNCGTDSVIVELIKDGVPDFPFLILKIDEHT
                     GEAGINTRIEAFRDMLERRLFNESNISTHG"
     gene            7877..8974
                     /locus_tag="PKH42_00370"
     CDS             7877..8974
                     /locus_tag="PKH42_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOA18908.1"
                     /translation="MKATFPHMGNIYIAVKALFDGLDIDYVIPPQNSTEALNIGSKYS
                     PEEICLPFKIMIGNYIQAIERGADTVILTGSCGPCRFGEYCEIQMNLMKNLGYDLNFI
                     VLDMPKDIGLKELLKRLVKVGSTSKKSSFEKLKAAKNAIEIMNLIDETESLAHYLAGY
                     ERNKGECKRLLHKCLKEAVKLSNPYEMLSLIKEYRNKLKYIPVDINKDPLKIALIGEI
                     YTVIEPYSNFYIEDKLMDYGVSSKRGMTPSWWLKDMALKPSKLNSLKIKKSSREYLSM
                     EIGGHARECIGEAILAYKEGFDGAIQIFPMGCMPEIVSKSILPTISKDYGFPIMSLVV
                     DDMTGEAGYITRVEAFIDLLERRRDNVLYGS"
     gene            8955..9926
                     /locus_tag="PKH42_00375"
     CDS             8955..9926
                     /locus_tag="PKH42_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOA18909.1"
                     /translation="MYYMGVDVGSVSTDIALIDNNYELIEKIYLRTKGNPIKAVQEGF
                     KILKNKYEDSEIYAVGTTGSGRMIASAIIGADVVKNEITAHATAALEIDKDVKTIIEI
                     GGQDSKIIIINNGIVVDFAMNTVCAAGTGSFLDRQAERLEIPIEEFGDYAMKASTGVR
                     IAGRCTVFAESDMIHKQQLGYNHAEIIRGLCDALVRNYLNNIGKGKTILPKVLFQGGV
                     AANKGMKKAFENELGFEIYVPENFSMMGAIGAAIIAANKVKKTGKTNFRGLKLADNNI
                     FSRSFDCEGCPNKCEVVKIYENDDIIGYFGDRCTKWSSKICEKYKLA"
     gene            10087..12024
                     /locus_tag="PKH42_00380"
     CDS             10087..12024
                     /locus_tag="PKH42_00380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOA18910.1"
                     /translation="MLKEDDFPFLSARSYESTLNKIESAWENFIAGEIIPDFNLPRNP
                     ILESWKRSKEMGVPYNLKCAPLKLNEHDIMVERERQHNLFMIIDSYLDSIFKIFHDEN
                     MAVSFSNEKGIILSIRAGKSMLGNCHGINYLLGAGWSENDCGTNSIGTSLYTGKPIQI
                     YATEHYCKMAHSINCSSAIIKEPSSEIVLGALTITTHKNIVPAHTLNWAISEAQKIEN
                     AIEYHLQEESTSLMNCLFEMNEQPCIICKLDGKISKINKSAQMLGVKVGDRIENSFVF
                     PKYSRFDELFDNTYKTICCKTEQRYIVTIIPWRIGNNIIGAIAYFQKESLFLNQGNSA
                     KLTSDRSEFKCIIGKSPAFRQVIDLADKFSDSDMPILITGETGTGKEVLARAIHNNSC
                     RKEKIFLPVNCGAIPKELISSELFGYVEGAFTGTKKGGRIGKIEASSGGTLFLDEICE
                     MPIDMQIYLLRVLEEGTITRVGSNEAIPVNLRIICATNKDIRHEVEIGNFRKDLYYRL
                     NPVELHLPTLRDRGDDIILLAEHFLKLNSQDFKLSADAAELLMNYSWPGNVRELKSVI
                     NRAILLSKNSIITSNELFIHDKKQEIKYSKTHTEINEKRPTSDLIKQVIEECGGNKSL
                     AARRLKISRMTLYRRIKEYNL"
     gene            12270..14108
                     /locus_tag="PKH42_00385"
     CDS             12270..14108
                     /locus_tag="PKH42_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOA18911.1"
                     /translation="MKITKLISLLLALLMITSLAACTQPAAETPPEEPPAEETPPAVE
                     EGIKSGTYEVEGVGFGGKMSVKVTIKDGKIDDIEIGKNYETNGAGRVALEYVSQRIIE
                     GQTTNVEAVSGATISSSSLMALVRKALEEAGATKDQFSEEYKIARDIKTEREAEIVII
                     GAGGTGLAAAVTAGQEGASVIVLETNGFAGGNLLVSGGVYNSPEPEMQAKQGIEDSPE
                     LFVQQTLAGGDNIANPELVRILAYNALDGLNWLKELGNEFEDQVIQAPGALHPRSHNT
                     TAPLGSGIIKTYLEHIDKMDNVEILYETKGESLIQEDGRVVGVIAKNPDGSKLTVKAN
                     KGVIIATGGFSKNAQLVMEYRNAEKWPLLDENSVSTNMDSIRGEGIIMAREIGADVVD
                     MDQMQFLYLGQPKTGLLSGVFNVSAEVTVFVNKEGKRFVAEDARRDVISSAVFAQEDG
                     MMYMLHSAESLSDPANQLNIDGIPMKELLDIGAYGWKQGDTLEELAKEIGVPAENLVQ
                     TIAEYNEAVDTKNDPFGRTLLNVKMEKGPFYAIPRVPALHHTMGGLRIDTLARVLDTK
                     GEPIPGLYAGGEVTGGIHGGNRLGGNAVTDTVVFGRLAAQSAVAGK"
     assembly_gap    14110..14209
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14280..15398)
                     /locus_tag="PKH42_00390"
     CDS             complement(14280..15398)
                     /locus_tag="PKH42_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA18912.1"
                     /translation="MKNINVGMIGFGYMGKMHTMCYDIMKYYYNTDVNVNMYAVSKTS
                     KTSFPIKFEKIYPDYKSLINDENVDVIDICVPNFLHKEILIEAIKAGKHIYCEKPLAA
                     DIESANEVMEAYKKYNYNKTSRITFEYRFVPAIMRAKQIIDEGKIGNIIHFNFKYYGS
                     EFLDPNRPISWQSTKEKSGGGVLYALGTHAIDLIHYLIGDIDEVYADKRTHFKKRPLL
                     GTDKTVDVEIEDILNVQLYSGDKMGTLLLSQVAAGSGIDLTFEIYGEKGAIKFTHDNP
                     NVIYYFNNEDEKSPIGGLSGFKAIETTQKYGKAASFPPPRVNIAWTRYHIASIFDFID
                     AVANNTKAYPDIQDGYKVQLVTDAIYKSAEIKKSITVK"
     gene            15565..16329
                     /locus_tag="PKH42_00395"
     CDS             15565..16329
                     /locus_tag="PKH42_00395"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOA18913.1"
                     /translation="MKLKGKTAVVTGASSGMGREIAYYFAKEGANVVAVARRAERLNE
                     LVEQTKDFEGRVVAYSADITNSQKVNEMIDEAIKQFGRMDILVNNAGIMDDMSAVGDV
                     NDEMFDKVFNLNVKTILYSMRKAVKHFEEIGGGVIINISSIAGLYGGAAGATYTASKH
                     AVVGLTKNTAFMYALKNIRCNAICPGAVATEIGNSEFMKNINMEGVQRTGIGMPLNPR
                     TGEPSEIAKVAVFLASDDASFVNGQCIAVDGGWRAY"
     gene            16489..16980
                     /locus_tag="PKH42_00400"
     CDS             16489..16980
                     /locus_tag="PKH42_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOA18914.1"
                     /translation="MIRSAEQELYNILEQLNIKYKRYEHKPVYTVEEADNLDIDIPGQ
                     HCKNLFLRNKKGNIHYLVIADSQKHIDLKLLSKEIGSSGLSFASEERLYKYLGLKAGS
                     VSPFGLINDIERKVIVLIDRKLTDKSIVNFHPNVNTATIGVSYNDLEKFIKWHENRFI
                     YID"
     gene            17037..17639
                     /locus_tag="PKH42_00405"
     CDS             17037..17639
                     /locus_tag="PKH42_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOA18915.1"
                     /translation="MQYYKYGKKETDYLKERDSKLGREIDRIGIVKREVNPDIFDSLI
                     ASIISQQISTKAAFTVKNRLKESVGEITPETISKLDIESIQLCGMTMRKAKYIKNITQ
                     AVVSKTINLDNLNLLSDEEIINELIKFEGVGKWTAEMLLIHSFQRPDILSYNDLGIQR
                     GLKKLYSLEDISKEEFETYRKRYSPYNTVASLYLWKISED"
     gene            17788..18204
                     /locus_tag="PKH42_00410"
     CDS             17788..18204
                     /locus_tag="PKH42_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOA18916.1"
                     /translation="MKSYRKILTFNTKTRRAFINITPQIEECLKESGIKEGLLLCNPM
                     HITSSVFINDDEPGLHRDFEAFLEKLAPEKPYSQYLHNGYEDNADAHIKRTIMGREVV
                     VAITEEKLDFGTWEQIFYGEFDGKRAKRVLVKIIGE"
     gene            18229..18711
                     /locus_tag="PKH42_00415"
     CDS             18229..18711
                     /locus_tag="PKH42_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008795068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA18917.1"
                     /translation="MDVTIKPAYNEKEEILKLFKEYTDSILAEGEEVAACLKSQNYDK
                     EIIEIEEKYGLPGGRLYIACIGSETVGCVALKKIDDKYCEMKRLYVRPGNRGKHIGRK
                     LMEQVIFDAQGIGYKHIRLDTFPFMNVAIKMYKNYGFYEIERYNDNPAPTAVFMQLDL
                     "
     gene            18780..20009
                     /locus_tag="PKH42_00420"
     CDS             18780..20009
                     /locus_tag="PKH42_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOA18918.1"
                     /translation="MTFYNYLLSSLPNEFTAKIIYYILITIALLLACSIINFLVNRVV
                     LKYITKYIKNNKYKWDDVLLDAKLFQRIALIIPGLLIYMFASFYDNLQDIIQKVAIIY
                     ILVIISLVFRSLLAAIDSIYKQNPVSKERPIKGLLQIIEIAIYVVVGIAVISFLIDKN
                     PIYLLSGVGAVTAVVSLIFKDIIIGFVSGIQLVWNDMLRIGDWVEMPKYNTDGDVLDI
                     TLYSVKIQNWDKTISTIPTSAFITDSFKNWKGMREYGGRRVKRSFLIDLNTIKICSDE
                     MVERFKKIDFISEYMEEITKEIDNYNKENIINTELHINGKQLTNLRVFRAYLTNYLKN
                     TIELRPSSTTIVRQLAPTENGIPIEIYLFVADTRWVNYETIQADVFDHILAVIDIFEL
                     RMFQNPTGNDIKELMKK"
     gene            20121..21125
                     /locus_tag="PKH42_00425"
     CDS             20121..21125
                     /locus_tag="PKH42_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOA18919.1"
                     /translation="MVIISWLNRQLLKMEWLFELIRLLVENVFGLNIDDRLGGSIHFF
                     IYDVIKIFILLSVLIFTISYIQSYFPPERTKKILGRIKGVPANILGALLGTITPFCSC
                     SSIPIFIGFTSAGLPLGVTFSFLISSPLVDLASIILLASIFNWRTAIAYVVVGLLLAV
                     VSGTIISKLKFEEYVESYVFGKSSLDLEQEELTVNDRIEFSKNQVSDIIKRVWKYILI
                     GVGIGAAIHNWIPESLITAILGQDKWYSVIIAAVVGIPMYADIFGTLPIAEALIAKGV
                     GLGTVLTFMMGVTALSLPSMIMLKQVIKPRLLWTFIGLVSAGIIIIGYVFNAIGYMFI
                     "
     gene            21143..21382
                     /locus_tag="PKH42_00430"
     CDS             21143..21382
                     /locus_tag="PKH42_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA18920.1"
                     /translation="MIIKILGSGCKNCINLKENTEKALKETGIDAEIIKVENMKDIAS
                     YGVMRTPALVIDEKVVSYGKVLKPGDIAGLLEKYK"
     gene            21401..21667
                     /locus_tag="PKH42_00435"
     CDS             21401..21667
                     /locus_tag="PKH42_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOA18921.1"
                     /translation="MKKNYEQAALLFRALSDPNRLAIIDMIKTEEKCACKILEELQIT
                     QPTLSHHMKILCDSGLVNSRREGKWMHYSINKELFEEVKQLFDF"
     gene            21702..22250
                     /locus_tag="PKH42_00440"
     CDS             21702..22250
                     /locus_tag="PKH42_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOA18922.1"
                     /translation="MHIKKNNSCCSCSDGCCGSSAKPEINKREIVIDFLYLDVTVCER
                     CQGTDKSLDEAIGEVANILAATGIDVKVNKINVLNEELAIKHKFLTSPTIRINGKDIQ
                     MDYKESLCESCGDLCGDEVDCRVWIYQGKEYSSPPKAMIIEAILKEVYGEKSEKQEKK
                     EYVIPENLRKFYKSRRSKQNYS"
     assembly_gap    22293..22392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22507..24087)
                     /locus_tag="PKH42_00445"
     CDS             complement(22507..24087)
                     /locus_tag="PKH42_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOA18923.1"
                     /translation="MNNLDLIIKRRIFGIISHPDAGKTTLTEKLLLHGGAIRQAGTVR
                     ARKNARYAKSDWMEIEKQRGISVTSSVMQFEYDDKVISIMDTPGHNDFGEDTYRILTS
                     VDSAVMVIDAAKGIEAQTKKLFKVCSMRGIPIFTFINKLDREAKDPLELMQELEDVLG
                     LPSVAVTWPIGCGKEFEGIYDRQRNVVNLYKKKEIINLNEEDVKSNKLDGKIGPSNLT
                     NLRNAMELLDGAGNEFDIDLIRKGKLSPVFFGSALADFGVTEFLKHFLIMSPPPSSRK
                     TTTGEVHPTDDVFTGFIFKIQANMDPNHRDRLAFLRICSGTFVRGMNVTLTRTGKQMK
                     LSQSTHLMANERETVDTAYAGDVIGIYDSGNFQIGDTLTNGRDLIFFEPLPTFPPELF
                     SRVSPINSLKGKNFQKGVEQLAQEGAIQVYRNEYKEVILGAVGVLQFEVFKFRLVNEY
                     NTDIRMENIEFTVARWVKTDNPESLKRYQNSRCMLVFDHYERPVLLFTNQYALKSFQE
                     RYEDVELVEALDVVNMLG"
     gene            24418..25740
                     /gene="miaB"
                     /locus_tag="PKH42_00450"
     CDS             24418..25740
                     /gene="miaB"
                     /locus_tag="PKH42_00450"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOA18924.1"
                     /translation="MKGRRKTYHILTYGCQMNEHDSEKISGMLTSIGYEETDDEKSAD
                     LVIFNTCLIRENAELKVFGKLGEVKGLKRNKPDMLVAVCGCMMQKQEIREKLLKKFSF
                     VDIIFGTNTIHELPLLIYNVEINKRKSMGIVDNTESIYENMPKQRKYKYKALVNITYG
                     CNNFCTYCVVPYVRGREKSREPNEIIDEIKTLAEDGCKEVTLLGQNVNSYGSNLDKRV
                     TFAELLYEINKIEGIERIRFMTSHPKDLTDDLIKAIKECNKVCNHVHLPVQSGSNDVL
                     NRMNRKYTREHYLNLVEKLKTAVPDIAITTDIIVGFPGETEEDFLDTIDIVKKVEYDS
                     AFTFLYSIREGTKAAKMENQVPDNVKHERFERLLEVLYPIILDKNKQCIGKVFPVLVE
                     SFENGKLTGRTEHFRLVHFNGSKDLIGKIVNVKITNVKTFHTEGEIVE"
     gene            25778..28399
                     /gene="mutS"
                     /locus_tag="PKH42_00455"
     CDS             25778..28399
                     /gene="mutS"
                     /locus_tag="PKH42_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOA18925.1"
                     /translation="MAKYTPMMEQYLAIKEQYKDCILLYRLGDFYEMFFDDAITASRV
                     LEIALTGRDCGQEERAPMCGVPHHAVDGYIAKLIENGYKVAICEQLEDPSDAKGIVKR
                     DVVRIITPGTIIEQGMLDEKSNNYLCTIYLDKGFGMSYVDISTGELYVTENISLNDFL
                     KNSTHDLLIEEINKITPSEIISNNHIGNEVIDSMIYLSNVLSLDDYKQIVLKHFKVLS
                     LDSLGLKENYHAVISLGMLLNYLNETQKTSLDHINKLHFYTVGEYLQIDANTRKNLEL
                     IETIRGKKGPGTLYHVVDNTMTAMGGRLLKKWIEEPLKNIKHIYLRLDAVEELYENIM
                     TSNNIKEYLKSVYDIERLISRIVYGSCNGRDLNSLKQSLSNLPDLKSELNELSSQMFA
                     DIYSSFDELKDIFDLIDASIVEEPPISIKEGGIIKTGYNEELDELREITVNGKDWISN
                     LQAKEREATGIKNLKIGFNKVFGYYLEVTKSYLKDVPDNYIRKQTLANCERYVTPELK
                     EMEAKILNADEQIMKLEYELFLEIREFISRHVKRIQETAYNIAVIDAINSLAIAAVKN
                     NYVRPEMNKKGFIKITDGRHPVIERIMKNEMFVPNDTYIDNKEYRMSIITGPNMAGKS
                     TYMRQVALIALMSHIGSFVPAKKAEICIIDKIFTRVGASDDLSQGQSTFMVEMSEVSN
                     ILKNATSDSLLILDEIGRGTSTYDGLSIAWSVVEYITKKLKAKTLFATHYHELSELES
                     KLKSVKNYKILIKEEHDEITFLRKITEGSIDRSYGIQVANLANLPEEVIERAKEILIQ
                     LEESDINKPFARKKNRVNDNFQVSMFQANPAENEINKKYKELAENIKNIDIENITPMQ
                     SFKLLNDIIEKTKRIDT"
     gene            28417..30258
                     /gene="mutL"
                     /locus_tag="PKH42_00460"
     CDS             28417..30258
                     /gene="mutL"
                     /locus_tag="PKH42_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOA18926.1"
                     /translation="MAIINLLDNETINKIAAGEVIENPKSIVKELVENSIDAGADEIT
                     IEIKNGGKSFIRVTDNGKGIEKDQVENAFKRHCTSKISSSDDLNSLFTLGFRGEALAS
                     IAAVSKVEIITRTNDDIYGIKMTIEGGKIISKEDIGCPVGTTITVTDLFYNVPARKKF
                     LKSDISESSNINEIVVSLALSKENISFKYINNNNVAYRTPKTNNLLNTISSLYEKDLY
                     NSLLKIDYKGDVLGITGYTTNLNYYRGNRKQQIVFVNGRVIKHRRMSYFIEEAYNTLL
                     PKNKYPACFIKIEIAPALVDVNVHPAKTEVRFQNEEMVLNEIKNAVYSALHSKSIMKE
                     VKGETLLHDSKDINNLRIFDRSSGKSVILGGLENKFTEETEETKNIRITEVEVSNKDI
                     AVMESLKPYDKFEFKQQTFIKAENDEDKLPELFVIGIVMDTYIICENPEKTCIFMIDQ
                     HAAHERINYEKFLNQYNSREILIQEIVPEVINLSYDDYETAMSNIEIFEYLGISVENF
                     GNNAILINNIPTIFKDSNLKDVFYAILDNLKDITKSNLDFVMEKIIKEACVKSVKSGD
                     RLHMSEVRALLNDLRMTDNPYTCPHGRPVIIKMTRYEIEKMFERIQS"
     gene            30268..31218
                     /gene="miaA"
                     /locus_tag="PKH42_00465"
     CDS             30268..31218
                     /gene="miaA"
                     /locus_tag="PKH42_00465"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOA18927.1"
                     /translation="MNKIIVIVGPTAVGKTYISVELAKKLNTEIISADSMQIYKGMNI
                     GTAKITEEEKQGIIHHMIDIINPDEEYSVSEFKYDAEKIIDRLLSENRIPIIVGGSGL
                     YVNSLIYDLDFGNTKSNKKLREYYTYYYKEHGEDALYDKLLKIDPVAAEKIHKNNIKR
                     IIRALEVYDITGVKFSELNTDIRKKSNKYDCILIGLSMERKVLYERINQRVDEMLSKG
                     LVDEVSSLIKKGYGKNLVSMRGIGYKEIIEYLEGNTDYEEAVNTLKQNTRRFAKRQYT
                     WFLKDENVKWFSMDNPSEIDKTLDSIVEYISKKIDSISNQ"
     gene            31310..31546
                     /gene="hfq"
                     /locus_tag="PKH42_00470"
     CDS             31310..31546
                     /gene="hfq"
                     /locus_tag="PKH42_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOA18928.1"
                     /translation="MNIINLQDTFLNKVRKEKITITLYLNNGFKLTGTVLGFDNYVIF
                     IETDKGQQLVYKHAITSILPFKQVRLFFDEPEEQ"
     gene            31546..32844
                     /locus_tag="PKH42_00475"
     CDS             31546..32844
                     /locus_tag="PKH42_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOA18929.1"
                     /translation="MLTENKLCKQFGISEAVYNYVTKIEKEIKKNHFEKIDKIRELNQ
                     YKVIHAMQENKLDYTNFYWNTGYGYGDVGREKVEKIYASVFKTEDALVRPSIASGTHA
                     LYLTLSGILQHGDEVISISGRPYDTLLTVLGVQGKEPNNLSEAGIIYKEIPLYKNDID
                     WESAIKEVSMKTKLLMIQRSTGYSFRPALTIEKIKNAIERIRQVYPNIYIMVDNCYGE
                     FVDEMEPSDVGADVVVGSLIKNPGGGVALSGGYVVGKKVLIDRIANRLTAPGVGKEIG
                     LTFGTTRNTLQGLFFAPHIVSESLKGALLFGGVYSSLGFEITPTVEEDRSDIIQAIKF
                     NDKNMVIKICEAVQAASPVDGYVSPIPWDMPGYTNQVIMASGAFVSGSSIELSADAPM
                     RPPYIAYIQGGLFYDHVKLGVMLSLEKMMEFNEIKNKIFN"
     gene            complement(32915..33538)
                     /gene="lexA"
                     /locus_tag="PKH42_00480"
     CDS             complement(32915..33538)
                     /gene="lexA"
                     /locus_tag="PKH42_00480"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOA18930.1"
                     /translation="MSYEGLSDKQVKILQYIKDELTLRGYPPSIREICKAVGLNSTSS
                     VHAHLNTLEEKGYIKKGTNKRRALELIDLDDICCDMPKKEIINLPIIGTVTAGSPILA
                     VENIDDTLPISIDFVGNKESYVLKVKGDSMIEAGILHGDYVIVNSQRNAKNGDIVVAL
                     IGDEATVKTFYKEKDHIRLQPQNSSMDPILIKEPYILGVVKAVVRKY"
     gene            33768..34070
                     /locus_tag="PKH42_00485"
     CDS             33768..34070
                     /locus_tag="PKH42_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA18931.1"
                     /translation="MIILNKYKVSDSKKFKRFMFLSILLISIITITSITTIKAYSKDI
                     PQYDYVRVQHGDTLWSIAKDYAGSKDIRQVVYEISELNKIHNNLIHIGDIIKIPLN"
     gene            complement(34073..35053)
                     /locus_tag="PKH42_00490"
     CDS             complement(34073..35053)
                     /locus_tag="PKH42_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOA18932.1"
                     /translation="MYINYNDLCPSPLSDFLEYSIAIKGRADKTVSQYFLDLRTFYRF
                     LVIKFKLSPDVENFEEIDISPVTLDIIKKVKIMDLHSFIAYTDRERKNSNRTKARKMS
                     ALRSYFKYLHTIVKLIPENPALELETPKNKSRLPVALTLDESRSLLSSISGSNEKRDY
                     AIIMLFLNCGLRLSELISIDIDKIKGDTLTVIGKGNKERTIYLNDVCVKAVEDYLEVR
                     QEPKKGHEKALFISNRKTRFSQKGIQHMLDKYLTEAGLSGKGYSPHKLRHTAATLMYQ
                     YGHVDIRALQEILGHESISTTQIYTHINKELLRDAVKQNPLNDMNDDESL"
     gene            complement(36019..36672)
                     /locus_tag="PKH42_00495"
     CDS             complement(36019..36672)
                     /locus_tag="PKH42_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18933.1"
                     /translation="MKDSGCKKREYRDNPNVINNINTCTDTKNSNNLATGNSCTPEEL
                     IRNGGFEEVGIQTVFANWNDETEGLDINIRPSTSITYEGRQSANFDIGPVPGNTTDRA
                     TLTQTVSVNPGCYLTLSFAERLAEKPVDIELYFSAEVFYISGSNEVELIKIEKIAPRA
                     VTVESSAFSYHEKTSDIIVPQNVNFVIVRFTVSINTINASNNDTDLYLDGVSLRASR"
     gene            complement(36838..37623)
                     /locus_tag="PKH42_00500"
     CDS             complement(36838..37623)
                     /locus_tag="PKH42_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOA18934.1"
                     /translation="MSRNFTNEELSKMLYEALKIQKPIDVISDIDNEVTVEDAYDIQL
                     LNVKRALEDGEIVTGKKIGLTSKGMQELLGITEPDYGHLYKSTEVKNGGTVDTKTLIR
                     PKVEGEVAFVLKEDLKGPGITVEDVYKATDYICASIEIVDSRIKDWKIKLVDTIADNG
                     SSALYILNDKKVKIDDVSLPDIRMTLYKNGEVVNSGTGKDVLGNPAISVAWLANKLCE
                     YGITLKKGEIILSGAITASPEAFKGDMFECVFPELGNVKVEFK"
     gene            complement(37681..39285)
                     /locus_tag="PKH42_00505"
     CDS             complement(37681..39285)
                     /locus_tag="PKH42_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOA18935.1"
                     /translation="MNFNPAEYKYPSRRKVVYGYNGMVATSQPLAAQAGLEILKNGGN
                     AVDAAIAAAACLTIVEPTSNGLGSDAFALVWFNNKLHGLNGSGYSPYSISIEKLKERG
                     FTEIPKYGIIPIMVPGAPAAWVELSQKFGKLSLIEVLQPAINYAKYGFPVSVTVKKAW
                     DNAYKIYKKENKEEFKYWFDTFTYKGRTPNYGELWKLPYHAETLESIGKTHGNSYYKG
                     AIADKIDSFFKKYDGYLRKEDLEDYKTDWVEPISVNYRGYDVYEIPPNGQGLVALMAL
                     NILKNFDFTYKDKSDTYHKQIEAIKLSFDDGQKYITDIRYMKADIKQLLSDDYGRDKG
                     SLITDNALNLKETDPNSSGTVYLCTADGEGNMVSFIQSNYTGFGSGIVIPETGIALNN
                     RLHNFNYVNQHINCLMPHKKTYHTIIPGFLMKDGKALGPFGVMGAFMQPQGHVQVVSN
                     LIDFHMNPQEAIDAPRWQWIKGMTVEVEQDVPKYIINELINRGHDVKIQLNESSFGRG
                     QMILRTKEGTLCGGTEKRADGHIALW"
     gene            complement(39401..40936)
                     /gene="rny"
                     /locus_tag="PKH42_00510"
     CDS             complement(39401..40936)
                     /gene="rny"
                     /locus_tag="PKH42_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOA18936.1"
                     /translation="MFEEIIVGIICAIIGIIIGFFIRKNISESKIGSAELEAKRIIED
                     ANQKAETRKKEILVEAKDEAHRLRNEVERENKERRNEILRAEKRLIKKEEMLDSKSLV
                     VEKKDELLQKKLKEIEITQEKLDAIHQKQIEELEKISGLSTEEAKSILINNIKKEAEQ
                     EAAVAVKEIEKESKETAERKAREIITYAIQKCSADHVAETTVSVVDLPNDEMKGRIIG
                     REGRNIRALEQLTGIDIIIDDTPEAVVISGFDPIRREVARLALEKLIADGRIHPARIE
                     EMVDKAKKEVDQIIKDEGEQATLDTGIHGLHPELVKLLGRLKFRTSYGQNVLKHSIEV
                     SYLAGIMAAELGADVKVAKRAGLLHDIGKAVDHEVEGPHVTIGEDLAKKYKESKAVVH
                     AIAAHHGDTEPQTIEAVLVQAADAISAARPGARRETLETYIKRLEKLEEISNSFEGVE
                     KSFAIQAGREVRIMVKPEVTSDASIVLIAKDIVKKIEEEMEYPGQIKVNVIREIRATE
                     YAK"
     gene            complement(41247..42275)
                     /gene="recA"
                     /locus_tag="PKH42_00515"
     CDS             complement(41247..42275)
                     /gene="recA"
                     /locus_tag="PKH42_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOA18937.1"
                     /translation="MEKDKALELAIAQIEKQFGKGSIMKLGENAKLNIEAIPTGALPL
                     DIATGIGGVPKGRIIEIFGPESSGKTTVALHIVAEAQKKGGIAAFIDAEHALDPAYAK
                     ALGVTVEDLIISQPDTGEQALEIAEALVRSGAIDVIVVDSVAALVPKAEIDGEMGDSH
                     MGLQARLMSQALRKLAGAISKSNAVAIFINQLREKIGVMFGNPETTTGGRALKFYASM
                     RLDVRRVETLKKGDDVYGNRTRVKVVKNKVAPPFKQAEFDIIYGKGISKEGCILDMAV
                     EAGVVNKAGAWYSYENTRIGQGRENSKDYLLSNPELMDEIEKKVLEKYELGDLKVASD
                     ANDPLSYE"
     gene            complement(42342..42905)
                     /gene="pgsA"
                     /locus_tag="PKH42_00520"
     CDS             complement(42342..42905)
                     /gene="pgsA"
                     /locus_tag="PKH42_00520"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOA18938.1"
                     /translation="MKIVDWFKNLNLPNKLTVIRVISIPLFLLFLYISKGILRFLPLL
                     IFAGAAVTDLVDGYIARRDNLVSDFGKFMDPLADKLLTASAFIAFVELGYISAWVVVL
                     IISREFLVSGFRTLAASKGVTIAANAWGKIKTVFQMVLIIAILLNYTGYFGFTNTIIA
                     PLIAITVLLTITSGAVYINENIGVIKN"
     gene            complement(42883..44229)
                     /gene="rimO"
                     /locus_tag="PKH42_00525"
     CDS             complement(42883..44229)
                     /gene="rimO"
                     /locus_tag="PKH42_00525"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOA18939.1"
                     /translation="MKKIYMHSLGCSKNLVDSENMLGILSKKGYKVTDYADKADYIII
                     NTCSFINDAQQESVNAILFAADIKNKVNKKTKIIVTGCLSQRFGEELSKEIPEIDIII
                     GTAGFERIDEYINEYEKNKELLIDISLELDIDKELPRNTMTEAWVSYLKIAEGCSNNC
                     TYCVIPKLRGLFKSRRIENLVEEAKVLASKGVKEIIIIAQDTSKYGIDIYGEKKLHEL
                     IRKISEIEGIRWIRIHYLYPEDIYDELINEFKNNKKLLKYFDIPMQHINDRILKRMNR
                     KTSREQITSLIQKIRVEVKDAVIRTSLITGFPGETWDEHEELKNFLKEYRLDRVGIFK
                     YSREEDTPAYKLPGQIDEDVKEQRLNELMEIQQEISYENNLLKIGKTYDVLIEEKDAE
                     NVYVGRTYMDSIEIDGCVYVASNKELRLGEIYKVRINDALEYDLIGDVSDEDSRLV"
     gene            complement(44237..46564)
                     /locus_tag="PKH42_00530"
     CDS             complement(44237..46564)
                     /locus_tag="PKH42_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOA18940.1"
                     /translation="MAKTTTNKTNKTNKKTSINKKSTSSKASGKNILIKEITGIFIIS
                     FAVFMIIVATKEAQTGIIGKSINTFTHSAFGAGAKVLPYFILAVGILRLLNIIILNDN
                     NQIAAIIGFFLCFIIFKAISDADVYAHMADSNDFQELIKASAEFGMRNKGGGVFGNAI
                     TFLFVKLIGKNGTYVALGALIFSSIVLLTNQSIIGILKLIKNYIQKFTNLIYRFIFTE
                     SDESKTKKEKPAKKQQEESIQIYDYMSAVQENKKAEITQLKIEDIQKNENIPEASKDV
                     PIEESKEKFKQKEITDEIITISDVKTDNYKLPSIKLLDPMPLHIRGDKEDLKKDGEKL
                     IETLNNFNIPCNILQINKGPTITRYEIQPAPGIKVSRITALSNDIALALATSEIRMEA
                     PIPGKAAIGIEVPNRAKTSVYLRSMIESREYRNAPTKIPFALGKDIAGKNIIASIDKM
                     PHLLIAGATGSGKSVCINSLIMSILFKAKPDEVKLILIDPKVVELSNYNGIPHLLIPV
                     VTDPRKAANALNWAISEMTNRYKIFAQSSVRDIDSYNEKMVTENKEKMPKIVIIIDEL
                     SDLMSVASAEVEEYITRIAQLARACGMHLVIATQRPSVDVITGVIKANIPSRIAFAVS
                     SHIDSRTILDTGGAEKLLGRGDMLYHPIGLPKPVRIQGTYVSDDEIKRVVDYIKEQQI
                     EIKMFKQNEIIKEIENQTNNEEVDEYLEQAIETVVNDGQASVSYIQRKFRVGYSRAAR
                     IIDQLEERGIVGGHEGSKPRKVLITKEELDKMRGN"
     gene            complement(46696..47481)
                     /locus_tag="PKH42_00535"
     CDS             complement(46696..47481)
                     /locus_tag="PKH42_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOA18941.1"
                     /translation="MENNKMNIPQQKTQPSLNQAQSSTEPINPFIFSGTPNDNEDGSQ
                     PIENVKQVGSITQVEPVKDICFISIIGEIEGHSSLPPQSKTTKYEHIIPMIIGAEMDP
                     QIKGILLLINTMGGDVEAGLAISELIASITKPTVSIVLGGGHSIGTALAVSADYSFIA
                     PTGTMTIHPIRTSGLVIGVPQTFRYFQKMQERITNFIVSNSHVDKQALLDYMYDTDEI
                     ANDVGTVLNADEVVNIGLIDEIGGFSKAMKKLRELIDNMEEEG"
     gene            complement(47563..47796)
                     /locus_tag="PKH42_00540"
     CDS             complement(47563..47796)
                     /locus_tag="PKH42_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOA18942.1"
                     /translation="MKLSELYSKEIINVDTGENLGIFGDCDLVIDEKTGEIISFISGG
                     NSPFAFFKEPKRKEILWKNIKKIGSDMIIIEND"
     gene            complement(47836..48270)
                     /gene="dut"
                     /locus_tag="PKH42_00545"
     CDS             complement(47836..48270)
                     /gene="dut"
                     /locus_tag="PKH42_00545"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014032722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOA18943.1"
                     /translation="MKIKVVKKNPFKLPEYETKGSAGVDLQAYVDAPLILKPMERALV
                     PTGLFIELPEGYEAQVRARSGLAIKHGISLVNGIGTIDSDYRGEIKVILINLGGEDFT
                     INNGDRIAQMVIMKHERADFELVEDLNETERGQGGFGHTGIH"
     gene            complement(48260..49540)
                     /locus_tag="PKH42_00550"
     CDS             complement(48260..49540)
                     /locus_tag="PKH42_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOA18944.1"
                     /translation="MIKKHTLDNGLRIVMEKIDYVKSASIGIWVNVGSYNENEETNGL
                     SHFIEHMLFKGTKNRKANEIAEEIDNLGAQMNAYTSKECTSFYVKVLEENINEALDIL
                     SDMFFNSLFSPEEIEKEISVVIEEIKMYEDSPEDIVYDKLSEIIFNDSPMAFNILGTE
                     KNLKTFTSDKVKKYMEENYSPSNTVISIAGSFDEDEFIKLIEEKFKHWENKDVKRLNK
                     NSDYQRKIRGINKDLEQLHICIGNKTFGRHDKNYHPLLVLNNLLGGTASSRIFQEVRE
                     KKGLVYSIYSFVTNYSDTGIFSIYAAMSYEQVEEALRTILKEMASIKNGNIKEAEFNR
                     AKQQIKGNFILSLESTSSRMSAIGRRELLYNEILYPDEIIESINNVKIDDVLDVAKEL
                     FNIDNLSITFAGNLHKHSDIEDKIYKVLGEEYEN"
     gene            complement(49600..49782)
                     /locus_tag="PKH42_00555"
     CDS             complement(49600..49782)
                     /locus_tag="PKH42_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18945.1"
                     /translation="MKFFCNVAFIRKLLIIIFLVIIIILLIIGRFRTMRVKMVGKEVW
                     GNTLMHCERDTHHNDG"
     assembly_gap    49822..49921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(49951..52041)
                     /locus_tag="PKH42_00560"
     CDS             complement(49951..52041)
                     /locus_tag="PKH42_00560"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOA18946.1"
                     /translation="MEEKIFKYILAGRELKVTIGKIAELANGSCIVQYGDTVIMANVA
                     ASKEPREGIDFFPLSVDFEEKLYSVGKIPGGFIKREGRPGEKAILTSRLIDRPLRPLF
                     PKGYRNDVAVTVTVLSVDQDCSPEIAGMIGSSIALSISDIPFEGPTGAVNIGLIDGKF
                     IVNPNSKEREITELDLTVSGTKDAVMMVEAGANEVKEEVMLDAIMFAHEEIKKLCDFI
                     IDITAQVGKEKSAYTCFVCDENIEKEIREYAAPIINEAIIEEDKLKRQENIDNFKAQA
                     KEIFLEKYPENETDIDEALYTIVKEKIRENILLRGIRPDNRGIDEIRKISCEVGILPR
                     THGSGLFTRGQTQVLTVATLGVSSDEQTLDGISEETSKRYMHHYNFPSYSVGEARPSR
                     GPGRREIGHGALAERALEPVLPSEEEFPYTIRLVSEVLSSNGSTSQASVCGSTLSLMD
                     AGVPIKAPVAGVAMGLMKEGDNVVVLTDIQGLEDFFGDMDFKVAGTREGITAIQMDIK
                     VHGIDKEILSKALQKAYVGRMFILDKMLECIDKPREEVSKYAPKIISMSIDPEKIRDV
                     IGSGGKVINKIIEETGVKIDIEDDGSVLIAAENVENAYKAKRIIEGIVKEIEVDEIIL
                     GKVVRIEPYGAFVELSDNKDGLLHISQIDNKRVDKVEDVLKIGDEVLVKVIGIDEKGK
                     IKLSRKAAMQQVQK"
     gene            complement(52139..52402)
                     /gene="rpsO"
                     /locus_tag="PKH42_00565"
     CDS             complement(52139..52402)
                     /gene="rpsO"
                     /locus_tag="PKH42_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOA18947.1"
                     /translation="MNKEIKTQIIEQYRINEKDTGSPEVQIALLTYRINNLTEHLKEN
                     KKDHHSRRGLLQMVGRRRGFLNYLKDNDIERYRSIVAKLGLRR"
BASE COUNT        17436 a         8631 c         9375 g        16795 t
ORIGIN      
        1 ttgtttaact gtgccaaaaa attttcttgt tatatttaat aatatgtact ataatgttaa
       61 ggtgttgaat acataatagg gacattatgt gcaacagtaa atactaagtt tggtttcacc
      121 atacttatat attatgaaag gaaaacaata tgataatcgc aatagacgga ccgtcaggag
      181 ccggaaaaag cactgttgca agactattaa gcaaaaaact tggatatgag tacatcgata
      241 caggggctat gtaccgagct cttgcatata aagcatataa acaaaatatt gacataaatg
      301 aagctaatat tgcagaattg cttgaaacaa ccaatattac ctacaatgac aataaagtat
      361 ttttagacgg tgaaaatgtt gaaaacttaa taagaaatga agaaatatca acagcggctt
      421 caaaaatatc atctttaaaa attgtcagag aaaaaatggt tgaaatccaa agaaagattg
      481 caaaaaataa aaatgtggta ttggaaggtc gtgatattgg tacaattgtt tttccggatg
      541 cagagcacaa atttttcata actgcatcat tggaagagag agcaaaaagg cgttacgagc
      601 aattgaaatt aaacaatatt aaagctgatt acacaaatgt tatcaatgat atgattaagc
      661 gtgacgaaaa tgattcaaca agaaagtttt ctccccttaa acctgctgaa gatgcaatat
      721 taatagacac aacgaatatg gacttgaatg aagttacaaa gactattgca caatatatcg
      781 gaggaagcaa tgttttataa aatagcagtg attgtattaa aggttcttgt atttataatt
      841 tttgacttag aagtatataa cagagagaac cttgacaata tagacggtgg cttaataatt
      901 tgcggaaatc acagatccat gattgaccct gtaatattag cagtatctac taagcggcaa
      961 attcacttca tgggcaaaaa agaattgttt gaaaacaaat tcctatcatt tttattcaga
     1021 agtcttggtg cctttcccgt cgacaggaaa ggagtatcct tatcggcaat aaaaaactcc
     1081 cttaatgttt taaataaggg cggtatatta ggaatatatc cggaaggaac aagaattaaa
     1141 accggatttg acgaaaaaaa tgcaaaatcg ggaattgcct taatagctaa taaagcaaaa
     1201 gtcggaatta tgcccgttta tttggatgga ccatataaat tcagaggaaa actcagactg
     1261 tatataggag aagaaaaaga ctacttcaaa aattacagcg gcaggctgaa ttctgaagat
     1321 tatacggaaa taggaataga aattttaaag gatatttaca gattaaaaaa tgaaggtatg
     1381 aaatgaaaat aattgtttca gaatacaacg gtttttgcgg aggagtcaag gctgccgtac
     1441 tgaaagcgga taagattttg actgacgaaa aaaaactata ctgttacgga gaaataattc
     1501 acaacaaaga tgttgttgaa aaattaaaaa acaaaggaat ggttattgtt gacgatattc
     1561 ctgaaaacaa ggatgcaaaa tttcttatca gaagtcacgg tgtaggaaag aatatatatg
     1621 acagattgga agaaaaaaac atcgaagtta ttgatgcaac ttgtgtaaaa gtgaaaaaaa
     1681 tacataaaat agttgaagaa ttcaaaaata aagattacaa tataatcatt gtcggcaatg
     1741 agaatcatcc tgaggtacag ggaatatcgg gctggtgcaa ctatgaagcc gtaattatta
     1801 aaagccctga agaactaaaa aatataattg agccgacaaa aaaatactgt ttagtctgcc
     1861 agacaacatt taatttaaat acttttgaag aaatattaaa aactattgaa ataaacaatt
     1921 attctaatat aattgtacat aatacaatat gcgatgcgac aaagaaaaga caagaagcat
     1981 gcattgctct tgcttccaaa tgtgatataa tgcttattgt aggcggtaaa aacagttcaa
     2041 acaccaaaaa gttatatgaa ctgagcaagg aattgtgcga aaacacattc ttaatacaaa
     2101 attataagga aataccttat aactatattg ataaaaattc tattgtggga gtatccgcag
     2161 gtgcttcagc tcctgactgg ataattgagg aggttattaa tatgttggaa aatttgaata
     2221 acaacatgaa tgaaatgaac gagcaggcag aacatgcaga tttagctgaa cagtccgagc
     2281 cggcagaact gtctgataaa aaggaacaga cagaattagt agagcaatcc gaggaaacag
     2341 aaaagacaga aactgttgag cagactgata aagcagaaaa agtaaacagt atgcatgaat
     2401 tgcttgaaaa ttatggcgga gtagttaatc tcggaatcgg cgaaaaggtt aaaggaacag
     2461 taatatatgt aagtcctgat gccgtttctg ttaacatcaa ttataaatct gacggaatta
     2521 taacaagaga agatttttcc ttggaagaag tttccgattt aactgaagta gtaaaagaag
     2581 gagacgaaat tgatgcaatt gtagtaaaaa tttctgacca ggacggaaat gttgtactaa
     2641 ccagaaaacc attggaagaa cagaaaatct gggacgattt ggagcagatg agaatccgag
     2701 gagaaattgt ggaaattcct ataattgaag catctcagca cggaatttac ggaaaactaa
     2761 aaggcatcaa aggttttata ccaagaaatc aaatttcaat aaaaagaaat gttgaccctt
     2821 cggaatacgt aggcaaaaat ctgaaggtta agatattgga aacaaagaat aaaaaaggaa
     2881 gaagacagtt ggttttaact tcaagagctg ttgaaaaatt tgaaaaggaa attaaagaca
     2941 atgaagtatg ggaaaccatt caagaaggag aaatttacga aggcacggtg aaaaaccttc
     3001 aggattacgg tgcatttgta gaaatcaacg gtatcgatgg acttcttcac atcaatgaaa
     3061 ttgcatggac cagaattaaa cacccgtctg aagtgttaaa accgggcgac aaaataaatg
     3121 taaaggtaaa aaatgtagat aaagaaaata aaagactttc attaagttat aaagcaaccg
     3181 taaaaagccc ttgggcaaaa ttcttggata aatatcaaaa gggcgatatt gtaacaggag
     3241 tagtaactcg tattgttgac tttggagcat ttgttaaagt agatgatatt gaatgtctct
     3301 tacacataaa agatttggat tgggcaagaa cagaaaaggt aactgatatt ctccaggaag
     3361 ggcaggaggt aactgcaaaa atacttagca ttaccagaaa agacagaaag gttggccttg
     3421 gattaaagca attgactgag catccgtttg atctttatgc taagacacta aataaggacg
     3481 atataataca ggttgaagta acacgtatac tcttggacgg tatacatgtt aacgctaatg
     3541 gaaatattga ctgcttcata ccgattgcaa aggtgtcaac agaaaaatta agaacgccgg
     3601 cacaagttgt aaaggttgga gaaatagtag atgctaaagt gcaaagtatt gatatgaaag
     3661 gtaaaaacct taacctgaca ttaatattag atgacaaaag tgaagaaact gtagaaaata
     3721 aatcttatca atcagaagat acaaacttta ctataggaga atctatagga gatgcactgg
     3781 atgatttatt aaaataattt ctaatgttag gtcgcaagat ctaacatttt ttttgaaaaa
     3841 taggaggcaa aatgcttaaa gaatttttgt ttgatatatg ctccaaccaa tttgtttcag
     3901 gttttgagca tataaacggt gatattttga taaaatattt taagccttat actgattctt
     3961 ttgaaaagga caggcttgga agttatatat ttaaaagctc cggcagtaag gacaccaaaa
     4021 taatgcttgc tgcacatatt gacgaaatag ggcttatgat tacaggcata ttagatggag
     4081 gatttttgag tttcattacg gtgggaggat ttaatcctgc atcattgatt gcccaggatg
     4141 taacaatatt cggaaaagaa aaaatatttg gagttatagg aattaaaccg cctcatttaa
     4201 caagtgagga agaaagaaaa aaaccgctta aaacaaggga attatatata gatacggggt
     4261 acacaaaaga aaaacttgaa aaattaataa caattggaga tattgcaaca ataaacagag
     4321 aacctttatc attacaggga agcgttataa gcgccaaagc atttgatgac agggcatgtg
     4381 ttgcagttat gctcgaaaca gcaaaagaat taaaaaaaat atcacataaa agcaatgtat
     4441 attttactgc aacagctcag gaagaaacag gtttaagagg tgccaaaaca agcagttata
     4501 aaattgctcc cgatatcgga atagtcttgg atgtaggatt tggcaaaact cccgatatgc
     4561 cttccgattc ggctctctta ggtaaaggcc caattgttgc atacggagga aggctaaata
     4621 caaaacttac aaagaaattt gttgaaattt gcaagaaata caactatcct atgcagtatg
     4681 aagcaattcc tgttagaacc ggtacagaca ctgatgcact acaaatatca agagacggaa
     4741 ttccctgcat acttttatca attcctctca gatacatgca tacatcagtg gaaacaatag
     4801 atttaaagga tttagaaatt acaggaaaag ctatagcaag gtttataaat gaattggaca
     4861 attccgattt ggaggaaata ttatgttttt aaatgaatta actaaacttt ccggcgtatc
     4921 aggctgtgaa tttgaagtaa gaaattatat aaagagtaaa ctgtcggaaa tgggctgtca
     4981 attctatgta gataagcttg gaaatattat tgctcacaac aaaggcaaaa aaaataaaac
     5041 cattatggtt tccgctcata tggatgaagt aggattaata gttactaata ttgatagcga
     5101 cggttttatt aagtttaaag cagttggagg aattgaccaa agagttttaa actcaaaggt
     5161 agtattaata ggagaaaata atattccggg cgttataggc tctaaagcag tacatttgat
     5221 gactgaaaaa gaaaaagggg tctcccttcc aattgataaa ctatatatcg atattggtac
     5281 aagcacaaaa gaggaaacag aaaaattagt taatatagga gactacgcag cttttaaaag
     5341 cgattatgtt gaattcggcg acaatatgat taaagctaaa gccttggaca acagggtcgg
     5401 atgcagcatt atattggaga ttctaaaaga gaaattggat gtagattttt atggcgtatt
     5461 taccgttatg gaggaagtcg gattaagagg agcagaaaca gccgcatatc aattagagcc
     5521 ggacttagcc atagtattgg agggaaccgt atgcgcagat atgcctgaag tagaagaaca
     5581 tgaaaactcc accttaatag gcaagggagc agtattgtca ttaatggaca atgctacggt
     5641 atatgatatc gaccttgtaa gaagagttgc aaaaattgct gaagacaata atattgcata
     5701 tcagtacaga cgctcaactt caggggcaaa tgatgccggt gttatccata aaacaaaaaa
     5761 aggcgcaaaa gtagtgtcca tttcagttcc ttgcagatat atacattcat cagtaagcgt
     5821 tgcaagcaaa aatgacttaa tgagtgcttt aaagcttaca aaacaagtga ttattgaaaa
     5881 tcagaaaggg gaataaaatg tttaacagcg aattaatgaa aagattatcc gattgtttcg
     5941 gtccttcagg aagagaaaaa aggattcgcg aaatgattat ggatgaaata aaagattttg
     6001 cagatgaaat aaccatagac ccactcggta accttatagc aagaaaaaag ggtacaggca
     6061 aaaaaatact gttttctgct cacatggacc aaataggact gattataacc catgttgatg
     6121 aaaaaggatt attgcgtttt gcaaacgtgg gcggattaaa tgccaaagaa ttattgggac
     6181 tccgcatggt ttttgataat ggacttgaag gagtaatttg tcaagaagaa ttaaaagata
     6241 atgaaaaact ttcaacaagc aaattatttt tggatgttgc ttctaccgac aaagaattta
     6301 ttgcaaagaa ttttcgagta ggtgatatgt gtgtattcaa atcagaatat tacgaaacag
     6361 aggactgtgt aatatgcaag gctgcggatg acagaatagg ttgctatgtc ttaatcgaag
     6421 cactaaaaaa tcatccggaa acaaacaatg atgtatatta tgtgttttcg gtgcaggaag
     6481 aagtgggctg cagaggagct aaaacggcag gatacaacat taatccggat ttggcaatag
     6541 cagtagatgt aactgcaacc ggcgatacac ctgacggaat aaaaatggat gttaagttag
     6601 gcaatggagc agcaataaag ttaatggata aatcaatcat tacacatacg gaagtaaaag
     6661 acttattaac agatattgca gaaaaaaata gtattaaata tcaatatgaa attttggaat
     6721 tcggcggaac ggatgcagga ccaattcata catccagaga tggagtgccg agcggtgtaa
     6781 tatccattcc caccagaaat cttcatacct ccggagaaat attcaataaa ttcgacgtag
     6841 aagaatgcat aaaattagta ataggaacag ctgcaaaata accctcaatt attatgaaat
     6901 aacaaaaata tgttaaattt agggataata tcacaaatta taggaaataa tataatttgt
     6961 gatattttat ttaaaggagg atttatgaag gtaggaatac cgaaaggatt gatgtattgc
     7021 aaataccatc cttttttagt tacttttttc agtgaattgg gagctgaaat tattgtctct
     7081 gatgatacaa acaaaaacat tttggatgca ggagttaaat attgtgtgga tgatgcatgt
     7141 ttgccggtta agatatttca cggacatgtt gattcaatta aggataaatg tgatgtactg
     7201 gtaattccga gaattatgca attgtggaag aacgaataca tctgtcccaa attttgcggt
     7261 ctgccggaaa tggttatcaa tagcttcgat aaactgcctg taacaataac agagcctatt
     7321 tatgccacca ccaagaaaaa actttataat tgggctaaat catcgggaaa attcttaaga
     7381 aagaataatt atgaaataaa aagggcattc ataaatgcat taaatgcaca aaaagcacat
     7441 gaaatcggca tctatgattc cggttatgaa ataaatattg cattgacggg acatccctac
     7501 aatatttatg acagtttttt aaatatgaat ttggtaaaaa aaataaacgc gttgggaatg
     7561 gggataataa cggctgagtt tactggagaa gacattttgc aaaaagaatc ggaaaactta
     7621 tataaaagac ctttttggac ctttgtaaga gaaaactacg ggtttgccgt aaatgccgca
     7681 aaagaaaaaa acgtagacgg aataatatac atatcatcat ttaactgcgg aacagattca
     7741 gtaatagttg aacttattaa agacggtgta ccggattttc catttttaat attaaaaatc
     7801 gacgagcata caggggaggc gggaatcaat acaagaatcg aagcattcag ggatatgttg
     7861 gaaaggagat tattcaatga aagcaacatt tccacacatg ggtaatattt atattgcagt
     7921 taaggctttg tttgacgggt tggacatcga ttatgtaatt cctcctcaaa acagtacaga
     7981 agctttaaat ataggctcta aatattcacc tgaagaaatt tgccttcctt ttaaaataat
     8041 gattggtaat tatattcaag caatcgagcg cggtgccgac acggtaatac ttactggaag
     8101 ctgcggtccc tgccgttttg gggaatactg cgagattcag atgaatttaa tgaaaaactt
     8161 gggatatgac ttgaatttca ttgttttgga tatgcccaag gatataggat taaaagaact
     8221 gcttaagagg cttgtgaagg taggctcaac aagtaaaaaa agctcgtttg aaaaattaaa
     8281 agccgcaaaa aatgcaattg aaattatgaa tttaatagat gaaacagaat cgcttgctca
     8341 ttatttggca ggctatgaaa gaaataaagg cgaatgcaaa agacttttgc ataagtgttt
     8401 aaaagaagcg gttaaattat caaatcctta tgaaatgctt tcattaataa aagaatacag
     8461 aaataaactg aaatatattc cggtcgatat aaacaaggat cctttaaaaa tagctctaat
     8521 aggtgaaata tacacagtaa tagagcctta ttcaaatttt tatatcgaag ataaattaat
     8581 ggattacggt gtatccagca aaagaggaat gactccttct tggtggttaa aagacatggc
     8641 attaaaacct tcaaaattaa attctttaaa aataaaaaaa tcatcccggg aatatttatc
     8701 catggaaata ggaggtcatg ccagggaatg cataggcgaa gcaatccttg catataagga
     8761 agggtttgac ggggcaattc aaattttccc tatgggttgt atgcctgaaa tagtatctaa
     8821 gtcaattctt cctactattt caaaggatta cggcttcccg ataatgtcat tggtagttga
     8881 tgatatgaca ggagaagcag gttatattac gagagttgaa gcattcattg atttattgga
     8941 aaggagacgc gacaatgtat tatatgggag ttgacgttgg ctctgtaagt acggatattg
     9001 ctcttattga taataattat gagttgattg aaaaaatata tttaagaaca aaaggaaacc
     9061 ctattaaagc agtgcaggaa gggtttaaaa tactaaaaaa caaatacgaa gactctgaaa
     9121 tttatgcagt gggaactacg ggaagcggca gaatgattgc ttcagcaatt atcggtgcag
     9181 atgtcgtaaa aaatgaaatt accgctcatg caacagcagc ccttgaaatt gacaaggatg
     9241 taaaaaccat aattgaaata ggcggacagg attcaaaaat aataattatt aataacggaa
     9301 tagttgttga ctttgccatg aataccgtct gcgccgccgg taccggctca tttttagaca
     9361 ggcaggcgga aaggcttgaa atacctatcg aagaatttgg cgattatgca atgaaagcaa
     9421 gtaccggtgt aagaattgca ggcaggtgta ccgtattcgc cgaatcagac atgatacata
     9481 agcagcagct tggctacaac catgcagaaa taatcagagg actatgtgat gcactggtga
     9541 gaaactattt aaacaatata ggcaaaggca aaacaatact tccaaaggtt ttgtttcaag
     9601 gaggagttgc agcaaacaaa ggtatgaaaa aagcctttga aaacgagctg ggatttgaaa
     9661 tatatgttcc agaaaacttc agtatgatgg gagcgatagg agctgcaatt attgccgcta
     9721 ataaagtaaa aaagacaggc aaaacaaatt tcagaggcct taagcttgcc gataacaata
     9781 tattttcaag aagctttgat tgtgaagggt gcccgaataa atgtgaagtg gtaaaaattt
     9841 atgaaaatga tgatataatc gggtatttcg gtgacagatg cacaaagtgg agcagtaaaa
     9901 tatgcgaaaa atacaaatta gcataaaata tcagtcaaag gatatcatca cttgttactg
     9961 ccaaatctca acgtgcttat aatgcaaaga atgctccatt tcatccgtat tgacagggta
    10021 tataaaaaat tgtataatca attataatcc gtaataaaaa tttaagtgaa ataaggtgtt
    10081 aaacctatgc ttaaagaaga tgattttcct ttcctgtcag ctcgaagcta tgaatcaaca
    10141 ttaaataaaa tcgaatcagc atgggagaat ttcattgccg gtgaaattat ccctgatttt
    10201 aacttgcctc gaaatcccat cttagaatca tggaaacggt caaaggaaat gggagttcca
    10261 tacaatctta aatgtgcacc tttgaaatta aatgaacatg atataatggt tgaaagagaa
    10321 cgacagcata acttattcat gattatagat tcatacctgg atagtatatt caaaatattt
    10381 cacgatgaaa atatggcagt ttctttcagc aatgaaaagg gtattatttt gagtatcaga
    10441 gccggaaaat caatgttagg gaattgtcac ggcattaatt atttgttggg agccgggtgg
    10501 agtgaaaacg attgcggtac taatagtata ggcacatctt tatatacagg caagccaata
    10561 caaatttatg caacagagca ttattgcaaa atggcacatt caataaattg ttcatctgct
    10621 attataaaag aaccttcttc tgaaatcgta ttaggagcat taactattac aacacacaaa
    10681 aatattgttc ccgcccatac gcttaactgg gctatcagcg aagcacagaa aatagaaaat
    10741 gcaattgaat atcatttaca agaagaatca acgtctttaa tgaattgttt gtttgaaatg
    10801 aatgagcagc cttgcataat ctgcaagctt gacggtaaaa tcagtaaaat aaacaaatcg
    10861 gcgcaaatgt taggtgtaaa agtaggggat agaatagaaa acagttttgt atttcctaaa
    10921 tactcccgat ttgatgaact gtttgataat acttataaaa caatttgctg caaaactgag
    10981 caaagatata ttgttactat cattccttgg cgtataggta ataatattat aggagcaata
    11041 gcatatttcc aaaaagaaag tttatttctt aatcaaggca actctgctaa attgacgagt
    11101 gatcgcagcg agtttaaatg tataatcggt aaaagtccgg catttagaca agtaattgat
    11161 ttagctgata aattttcaga ttctgacatg cccatactga taacaggtga aacaggaaca
    11221 ggcaaagagg tattggctcg tgctattcat aataacagct gccgaaaaga aaaaatcttt
    11281 ctgccggtaa actgcggagc aatacctaaa gagttaatat cttcagaatt attcggatat
    11341 gttgaaggtg cttttaccgg cactaaaaaa ggcggaagaa taggtaaaat tgaagcatca
    11401 agcggaggca ccctgttcct tgacgaaatc tgtgaaatgc ctattgatat gcagatttac
    11461 cttttaagag tattggagga aggtacaatt acccgagtag gttcaaatga agcgataccg
    11521 gttaatttac ggataatatg cgctactaat aaggatataa gacatgaggt agaaataggg
    11581 aattttcgaa aagatcttta ttacagatta aatcctgtag agcttcacct tccgacttta
    11641 agagatagag gtgatgatat tatattgctt gctgaacatt ttttgaaact aaattcacaa
    11701 gattttaagt tatcagcaga tgctgctgaa ttgttaatga attattcgtg gccgggaaat
    11761 gtacgtgaat taaaaagtgt tattaatcgt gcgattttac ttagcaaaaa ttcaataatt
    11821 acatcaaacg aactgtttat tcacgataaa aagcaagaga taaaatattc aaaaactcat
    11881 acggaaataa atgaaaaacg tccgacatcc gatttaataa agcaagttat tgaagaatgc
    11941 ggcggcaata aaagtcttgc cgcacgcagg ttgaaaattt caagaatgac attatacaga
    12001 agaattaaag aatataattt ataatgacta tgtgatgctt ggtcataagt atagttgcct
    12061 aacaggcaac ttttttatta atgttacata ttaccaagtg acagatgtta caatgttaca
    12121 taaaaagtga caccaataca atgaaaaacc gcaacaatta acacatgttt cttggcataa
    12181 aaaatgctaa ttattaatag aatgtttgtt taaattttca taaacaacat cagtaaacat
    12241 tattaattat tatctaaatg gagggaagca tgaaaataac aaaattgatt tcattattgc
    12301 tggctttact aatgattact tcattagcag catgtacaca gcctgcggca gaaactccgc
    12361 cggaagaacc acccgcggaa gaaactccgc ctgcagtaga agaaggtata aaatcaggca
    12421 cttatgaagt tgaaggtgtc ggatttggcg gcaaaatgag tgttaaggta actatcaagg
    12481 atggcaagat tgatgacatt gaaattggga aaaattacga aacaaatggt gccggaagag
    12541 ttgcattgga atatgtttct caacgtatta ttgaaggtca aaccaccaat gtagaagcag
    12601 tatcaggggc taccatttct tcatcaagtt tgatggcatt ggttagaaaa gcattggaag
    12661 aagcaggagc tacaaaagac caattttcag aagaatataa aatagctcgt gacattaaaa
    12721 cggagcgtga ggctgaaata gttattattg gagcaggcgg aaccggtttg gctgctgccg
    12781 ttactgcagg tcaagaaggt gcctcggtta tagttcttga aactaacgga ttcgcaggcg
    12841 gcaatctctt agtcagcgga ggtgtatata attcgcctga acctgaaatg caagcaaaac
    12901 aaggtataga agactcaccg gaactatttg tgcagcaaac tttggctggc ggggataaca
    12961 ttgcaaatcc ggaacttgta aggatattag catataatgc tcttgatggg ttaaattggc
    13021 taaaagaatt aggcaacgaa tttgaagatc aggtaataca agcgccgggt gctttgcatc
    13081 ctcgttcaca caatacgact gcaccgttag gatcaggtat aattaaaacc tacctggaac
    13141 atattgataa gatggataat gtagaaattc tatatgagac taaaggagaa agcttaattc
    13201 aagaagacgg ccgagttgtc ggcgttattg caaaaaatcc ggatggctct aaattgacgg
    13261 taaaagcaaa taaaggtgtt ataattgcaa caggtggttt ttctaagaat gctcaattgg
    13321 ttatggaata tcgaaatgcg gaaaaatggc cattactgga tgaaaattct gtatcaacaa
    13381 atatggacag tatccgaggt gaaggaatta tcatggcaag agaaatcgga gctgacgttg
    13441 ttgatatgga tcaaatgcag tttttatact taggacagcc aaagacgggg ctattatcag
    13501 gcgtatttaa tgtttcagca gaagtgactg ttttcgttaa taaagaaggc aagagatttg
    13561 tagcagaaga tgctcgtcga gatgtaatca gttcggccgt attcgcccaa gaagacggaa
    13621 tgatgtatat gcttcatagt gcagaatcat taagcgaccc ggcaaatcaa ctgaatattg
    13681 acggtattcc gatgaaggaa ttgttggata tcggagctta tggctggaaa caaggcgata
    13741 cattggagga acttgctaag gaaataggag tacctgctga aaatcttgta caaacaatag
    13801 cagaatacaa cgaagcagtt gatactaaga atgatccttt cggaagaaca ttattaaatg
    13861 taaaaatgga gaaaggaccc ttctatgcaa ttccgagagt gcccgctctg caccacacta
    13921 tgggcggatt gcgcatcgat accttggctc gtgtattaga tactaaggga gaacctatcc
    13981 cgggattata tgccggcggt gaagtaaccg gtggaatcca cggcggcaat agattaggcg
    14041 gtaatgcagt aactgatacg gttgtatttg gtcgtcttgc agcacaatca gcagttgccg
    14101 gaaaatgagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tatttaagaa
    14221 cagacccggg attaacccgg gtctattcta atattatatt aatattgaag agtaactttt
    14281 tatttaactg ttattgattt ctttatttca gctgatttat atatagcatc ggttaccagc
    14341 tgtaccttat atccatcttg tatatcagga tatgcttttg tattattggc aacggcatca
    14401 ataaaatcaa atatgcttgc tatatggtat cttgtccatg caatatttac tcttggaggc
    14461 gggaaagaag cagctttgcc atatttttga gttgtttcta tagccttaaa accgcttaga
    14521 ccgcctatag gggatttttc atcttcatta ttaaaatagt atatgacatt aggattatca
    14581 tgagtaaatt taatggctcc tttttctccg tatatttcaa atgttaaatc tattcctgag
    14641 cctgctgcta cttgagaaag aagcagtgtt cccattttat ctccgctata aagctgaaca
    14701 ttaagtatat cttctatttc tacatctacc gttttgtccg ttcccaataa aggtcttttc
    14761 ttgaaatggg ttcttttatc agcatatact tcgtcaatat ctcctatcag gtagtggatt
    14821 aaatcaattg catgagttcc taatgcgtat aaaactccgc cgccggattt ttcttttgtt
    14881 gactgccagc tgataggtct gttggggtcc aggaattcac ttccgtaata cttaaaatta
    14941 aagtgtatta tattgcctat tttaccttca tctattatct gttttgccct cattatggca
    15001 gggacgaatc tatactcaaa agtaattctg cttgttttgt tataattata ttttttatat
    15061 gcctccatta cctcatttgc tgattcaata tcagcggcta aaggtttttc gcaatatata
    15121 tgcttaccgg ccttaattgc ttcaatcaaa atttctttat gtaaaaaatt cggtacacaa
    15181 atatctataa catctacatt ttcatcattt attaggctct tgtaatccgg ataaatcttt
    15241 tcgaatttta tgggaaaact tgttttactt gtctttgaaa cagcatacat atttacattc
    15301 acgtcagtat tataataata tttcataata tcatagcaca ttgtgtgcat ctttcccatg
    15361 taaccaaatc caatcatccc tacattaata tttttcatcc ttttctcctc catatagtta
    15421 taaatttcaa cgcattcatt aaaaatatta tacaaacgat tgcagaaata atcaagggat
    15481 tcatctaatt gaaaaataat agtttcggat tatattagca gggtataaat aatgtatctc
    15541 aatattaaaa tataaggagg ttttatgaag ttaaaaggga agactgcagt cgtaacaggc
    15601 gcaagttcag gaatgggaag agaaattgca tattattttg ccaaggaagg tgctaatgtt
    15661 gtagcagtag cacgtcgtgc cgaaaggttg aatgaactgg ttgaacaaac taaagatttt
    15721 gaaggtaggg ttgtagcata ttctgcagat ataacaaata gtcaaaaagt aaatgagatg
    15781 attgatgagg caattaaaca gtttggcaga atggatattc tggttaacaa tgcaggcatt
    15841 atggatgata tgagtgcggt tggcgatgta aatgatgaaa tgtttgataa ggtatttaat
    15901 ttaaatgtaa aaaccatatt atattctatg agaaaggctg taaaacattt tgaagaaatt
    15961 ggcggaggag taataattaa tatttcctct attgccggat tgtacggtgg tgctgcagga
    16021 gctacatata cggcaagcaa acatgcagtg gtaggtttaa ccaaaaatac ggcatttatg
    16081 tatgctttga agaatataag gtgcaacgca atatgtcccg gagccgtagc gacggaaata
    16141 ggcaattctg aatttatgaa gaatattaat atggaaggtg tacaacgaac gggaataggg
    16201 atgccgctta atcctcgaac aggtgaacca agtgaaattg caaaagtcgc agtgttttta
    16261 gccagcgatg atgcatcgtt tgtaaatgga caatgtattg ccgttgatgg cggctggagg
    16321 gcttactaaa tttatttgac aagattgctg caaaaaatta cagatgaaaa tgtatatcat
    16381 tataataaaa gtaatattca aatatttctt gaatattgct tttattttaa tatatgttat
    16441 tatagtttaa gaaaaacaga tgaaatatat atttggaagg gattgaaaat gattaggtca
    16501 gctgaacaag agttatataa tatattagaa caactaaata ttaaatataa aagatatgag
    16561 cataaacctg tatatactgt cgaagaagca gacaatttgg atattgatat tccgggacaa
    16621 cactgcaaaa atttattttt aagaaataaa aaagggaata ttcattatct tgttattgca
    16681 gattcacaaa aacatataga tttgaaatta ttatcgaaag aaatagggtc ttcaggtcta
    16741 tcctttgcat ccgaagaaag attatataaa tatttgggac ttaaagctgg ctcagtaagt
    16801 ccttttggtt taataaatga tatagaaaga aaagtaatag ttttaatcga tagaaaacta
    16861 accgataaaa gtattgtaaa tttccatccg aatgtaaata ctgcaactat tggtgtttct
    16921 tataacgacc tggaaaaatt tataaaatgg catgaaaaca gatttattta tatagattga
    16981 gtcaatagaa tttataccga tattcaacat tgagttatag catatgggag attaacatgc
    17041 aatattacaa atatgggaaa aaagaaacag attatctaaa agagagggat tccaaattag
    17101 gaagagaaat agacagaatc ggaatcgtaa aaagagaagt aaacccggat atatttgatt
    17161 cgcttatagc aagtattata agtcaacaga tatctaccaa agctgctttt acggtaaaaa
    17221 atcgattaaa agagtcagtt ggagagataa ctccggaaac tatcagcaaa ttagatatcg
    17281 aaagcatcca gctatgcggg atgacaatga gaaaagccaa atatattaaa aatataactc
    17341 aagcagttgt aagcaagact ataaaccttg ataatttaaa tttattaagt gatgaagaaa
    17401 tcataaatga attaatcaaa tttgaagggg tagggaaatg gacggcagag atgctgctta
    17461 tacattcttt tcaaagaccc gatatattaa gttataacga tttaggtatc caaagaggat
    17521 taaaaaaatt gtattcatta gaagatattt ccaaagaaga atttgaaacg tacagaaaaa
    17581 gatattcccc ttacaatacg gtagcatctt tatatttatg gaaaatatca gaagactgat
    17641 aaatactagc ttccttatta tatagcaaaa tgagtttaca taatttatat tatgtaaacc
    17701 gcagccgcta catatttggt tgaattttaa cgataaataa tctataatag aggataatac
    17761 gaacaaaaat ttgaaaaagg agaatatatg aaatcttaca gaaaaatatt aacttttaac
    17821 accaagactc gtagggcatt tattaatatt actccgcaaa tagaggaatg tttaaaagaa
    17881 agcggcatta aggagggact gctcctatgc aatccaatgc acataacgtc cagtgttttt
    17941 ataaatgatg atgagcccgg cttgcatagg gattttgaag ctttcctgga aaaacttgct
    18001 ccggaaaaac catatagcca atatcttcat aatggatacg aggataatgc cgatgctcat
    18061 ataaagagaa caattatggg aagagaagtt gtagtagcca taacagaaga gaagttagat
    18121 tttggtacgt gggaacagat attctatggt gaatttgacg gaaaaagagc aaaaagagta
    18181 ttggtaaaaa ttattggtga atagctaaaa tatcaagagg tacttaatat ggatgtaaca
    18241 attaagccgg catataatga aaaagaagaa attcttaaat tatttaaaga atatacagat
    18301 agcatattag cagaaggcga agaggttgcc gcatgtttga aatcacagaa ctatgataaa
    18361 gaaataattg agattgaaga aaaatacggc ttgcccggag gcaggttata tatagcatgc
    18421 attggcagtg aaactgtagg gtgtgttgca ttaaaaaaaa ttgatgataa atactgtgaa
    18481 atgaaacgct tatatgtcag accgggtaat cgtggaaaac atattggaag gaaacttatg
    18541 gaacaggtta tttttgatgc acagggaatc ggatataaac atataagact tgatacattt
    18601 ccttttatga atgtcgcaat taaaatgtat aagaattacg gcttttacga aattgaaaga
    18661 tataatgata atcccgcacc gacagcagta tttatgcaat tagatttata gaatgtacta
    18721 gaactatgtt taatcaataa atgtactaat acacattaat ttttaaatag gagagatata
    18781 tgacttttta taattattta ttaagcagtc tgcctaatga gtttacagcc aaaattatat
    18841 attacatatt aattaccata gctttattat tagcttgctc cattataaat tttcttgtaa
    18901 accgtgtagt cttaaaatat ataacaaaat atataaaaaa caacaaatat aaatgggatg
    18961 atgtattgtt ggatgctaaa ttatttcaaa gaattgcctt aatcatcccg ggactgttga
    19021 tatatatgtt tgcttcattc tacgataatt tacaggatat tatacaaaaa gttgcaatca
    19081 tatatatatt agttataata tccttggttt ttagatctct attggctgca atagacagca
    19141 tatacaagca aaatcctgtt tcaaaagaaa gaccgataaa aggtctttta cagataattg
    19201 aaattgctat ttatgtggtg gtaggtatag ctgttatttc atttttaatc gataaaaatc
    19261 ctatatattt gttaagcgga gtgggagctg ttactgctgt tgtttcattg atatttaagg
    19321 atataataat tggttttgta tcaggaattc aacttgtttg gaatgatatg cttcgaatag
    19381 gagattgggt ggaaatgcct aagtataaca ctgatggcga tgtattggat attacattat
    19441 attcggttaa aatacaaaac tgggacaaaa caatatcaac aatacctaca tctgcattca
    19501 ttactgattc gtttaaaaat tggaagggaa tgagagaata cggtggaaga agggttaaaa
    19561 ggtcatttct aattgatttg aacactataa aaatatgctc cgatgaaatg gtagaaagat
    19621 ttaaaaaaat agatttcatt agtgagtata tggaagaaat cactaaggaa attgacaatt
    19681 acaacaaaga aaacattata aataccgaac tgcacataaa cggtaaacaa ttaactaatt
    19741 tgagagtttt cagggcttat ttaacaaact atttgaaaaa cactatagaa cttaggccga
    19801 gcagcactac cattgtcaga caattagctc caacagaaaa cggtatacca attgaaatat
    19861 atttatttgt tgcagataca aggtgggtta actatgaaac aatacaagca gatgttttcg
    19921 accatatatt ggctgtaata gatatttttg aacttaggat gtttcagaat cctaccggta
    19981 atgatataaa agaattaatg aagaaataaa tctcagcaaa taaactgtcg agattttatt
    20041 tcttatattg acaatcatct atgtaatgaa tataataata tatagatggt tatctatata
    20101 ttattatagg aggatataaa atggttataa tcagttggct gaacagacag ctgctcaaaa
    20161 tggagtggct atttgaatta attaggttat tggttgaaaa tgtatttgga ctaaacatag
    20221 acgatagact tgggggaagt atacatttct ttatatatga tgtaataaaa atttttatat
    20281 tgctttcagt acttattttt accatttcat atattcaaag ttatttcccg cctgaaagaa
    20341 caaagaaaat actaggaaga ataaaaggtg tacctgctaa tattctggga gctttacttg
    20401 gaacaataac acctttttgc tcatgctcat caattcctat ttttataggt tttacaagcg
    20461 ctggactgcc gctcggagta actttttcat ttttaatatc ttcaccgctt gttgatttgg
    20521 catctataat tttattggcc agcatcttta attggagaac agcaattgca tatgttgtag
    20581 tggggctttt gctggctgtt gtttcaggca caataataag taaattaaag tttgaagaat
    20641 atgtggagtc ttatgttttt ggaaaaagct cgcttgattt agaacaagaa gaattaacgg
    20701 ttaacgatag gattgaattt tcaaaaaatc aagtatcgga catcataaaa agagtatgga
    20761 aatatatact tataggtgtg gggataggag cagcaattca taactggatt ccggagtctt
    20821 taataacagc catactgggg caggataaat ggtattctgt aataatagct gcagttgtcg
    20881 gtattccaat gtatgcggat atttttggaa cacttcccat agctgaagct ttgattgcaa
    20941 agggagtggg attaggtacc gttttaacct ttatgatggg tgtaacagcc ctttcattgc
    21001 catccatgat tatgttaaaa caagttataa agcctagact cttatggaca tttatcgggc
    21061 ttgtatctgc aggaataata ataatcggat atgtatttaa tgccatcgga tatatgttca
    21121 tttaaaatat aaggagggta atatgattat taaaatattg ggttcaggat gcaaaaattg
    21181 tattaattta aaggaaaata cagaaaaagc tttaaaggaa acaggaattg atgcagaaat
    21241 aattaaagtc gaaaatatga aggatattgc aagctatggc gtgatgcgaa ctcctgcatt
    21301 ggttattgac gaaaaggtgg tttcatacgg aaaggtttta aagccgggtg atattgcagg
    21361 actattggag aaatataaat aatatttgga aggtgacagg gtgaaaaaaa attatgagca
    21421 agcagcatta ttgtttaggg cattaagcga ccctaatcgg cttgctatta tagatatgat
    21481 taagactgaa gaaaaatgtg catgcaaaat attagaagaa ttgcaaataa cccaacctac
    21541 cttatctcat catatgaaaa tattgtgtga ttcaggcttg gttaattcaa ggcgtgaagg
    21601 taaatggatg cattattcta ttaataaaga gttgtttgaa gaagtaaaac agttatttga
    21661 tttctaaatt taattaataa taaatataaa aggggtaccc tatgcatatt aaaaaaaata
    21721 attcatgttg ttcttgctct gatggttgct gcggttcatc agctaagcct gagattaata
    21781 agagggaaat tgttatagat tttttgtatc ttgatgtaac tgtatgtgaa agatgtcaag
    21841 gaacggataa aagtcttgac gaggctattg gtgaagtggc taatattctt gcagcaacag
    21901 gcatagatgt aaaagtaaat aaaataaatg ttctaaatga ggagcttgca ataaagcata
    21961 aatttttgac ttcacctaca atacgcataa acggcaagga tatccagatg gattataaag
    22021 aaagtctttg tgagtcctgc ggagatttat gcggagatga ggttgattgc cgtgtttgga
    22081 tttatcaggg aaaagaatat tcaagcccgc caaaggcaat gataattgaa gcaatattaa
    22141 aagaagtata tggagaaaaa tcagaaaaac aggagaagaa ggaatacgta attcctgaaa
    22201 atttaagaaa attctataag tctagaagat ctaaacaaaa ttactcttaa tatgcaatat
    22261 atgataaagg cggtaaggta taaccttacc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnaaattact cttaatatgc aatatatgat aaaggcggta aggtataacc
    22441 ttaccgccag attgctaaaa tactctttaa tgtcatcctg agcgtaagcg aaggatctcg
    22501 ctcttactaa cctaacatat ttacaacatc aagtgcttct accaattcta catcttcata
    22561 tctttcttgg aagcttttta atgcatattg gttggtaaac aataaaacag gtctttcata
    22621 atgatcgaaa acaagcatac agcgtgaatt ctgatatctt tttaaacttt ccgggttatc
    22681 tgttttaacc catcttgcaa cagtaaactc tatgttttcc atgcgaatat ccgtgttata
    22741 ttcgttaact aaacggaatt taaaaacttc aaactgtaaa actcctacag cacctaatat
    22801 tacttccttg tattcattcc tgtaaacttg aattgcacct tcctgagcta attgttcaac
    22861 acctttttga aagtttttcc ccttaagaga atttataggg cttacgcggc tgaacaattc
    22921 aggcgggaag gtgggcaagg gctcaaaaaa tattaaatct ctgccgtttg taagagtatc
    22981 gccaatttgg aagtttccgg aatcataaat acctattacg tcgcctgcat atgcagtatc
    23041 cacagtttca cgctcatttg ccatgagatg tgtggattgg cttaatttca tttgttttcc
    23101 tgttcttgta agggtaacat tcattcctct tacaaaagtt ccggagcaga ttcttaaaaa
    23161 agcaagtctg tcgcggtggt tagggtccat atttgcttgg attttaaaaa taaaacccgt
    23221 aaatacatca tccgtcggat ggacttcccc tgtagttgtt tttcttgatg atgggggagg
    23281 agacataata agaaaatgct ttaaaaattc tgttactcca aagtctgcta aggcagagcc
    23341 gaagaataca ggtgataatt tgccttttct gattaaatct atatcaaatt cattacctgc
    23401 accgtcaaga agctccatgg catttctcaa atttgtcaaa ttggaaggac cgatttttcc
    23461 gtctaatttg tttgatttaa catcttcttc attgagatta ataatttctt tctttttgta
    23521 taagttaact acatttcttt gcctgtcata aattccttca aattctttgc cgcatccgat
    23581 aggccatgtt acagcaacag aaggaagtcc cagtacatcc tctaattcct gcattaattc
    23641 cagcgggtcc tttgcttcac ggtctaattt gtttataaat gtgaatattg gaattcctct
    23701 catgctgcaa accttaaaca gtttctttgt ttgcgcttct ataccttttg cagcatctat
    23761 aaccattacg gcactgtcaa cagaggttaa aatacgataa gtatcttcac caaagtcatt
    23821 gtgtccggga gtatccataa ttgaaattac tttatcatca tattcaaact gcataactga
    23881 agaagtaaca gaaatgccgc gctgtttttc tatttccatc cagtctgatt tggcatatct
    23941 ggcatttttt cttgctcgaa ccgtacctgc ctgcctgatt gctcctccgt ggagcaggag
    24001 cttttcggtc aacgttgttt ttcctgcatc agggtgagat attataccaa atattcgtcg
    24061 tttgataatc aaatctaaat tattcatttt tttcctttct agccggtaat aattaatctt
    24121 taatattcta cactatttcg acaaaaatat aaagaaatat ttacaagtaa ttttgattcg
    24181 caaggatggt acactaatac tgaaatttag attcaatagt atcttgtata aaggctgagg
    24241 gaattttttt attgaacaaa aattcgtaaa atgatataat attcaagatg ctaaacgttt
    24301 agattagaaa gttggagata gatggacgaa agaaatattg atattataac agaagatata
    24361 aaattagacg gcgataattt attagaatta aagaacaata atgagcaatt cttcataatg
    24421 aagggcagaa gaaaaactta tcatatattg acatacggct gccaaatgaa tgaacacgac
    24481 tctgaaaaaa tatccggtat gctgacatcc atcgggtatg aagagactga tgatgaaaaa
    24541 agtgcagacc ttgtaatttt taatacatgt ttaataaggg aaaatgcgga acttaaagta
    24601 tttggcaaac ttggtgaagt aaaaggactt aagcgaaata aaccggatat gctggttgca
    24661 gtttgcggct gtatgatgca aaaacaagaa ataagagaaa aactgttaaa gaaattttca
    24721 tttgtagata ttatttttgg aacaaataca attcacgagc ttcctttgtt aatatacaat
    24781 gttgaaatca acaaaagaaa aagtatgggt attgttgata ataccgagtc aatatatgaa
    24841 aatatgccca agcaaagaaa gtataagtac aaagcattgg taaacataac atacggctgt
    24901 aataatttct gcacttactg cgtagttcca tatgtaagag gcagagaaaa atcaagagag
    24961 ccgaatgaaa taattgatga aattaaaaca ttggcagaag atggctgcaa agaagtgacg
    25021 cttcttggac aaaatgttaa ttcttacgga agcaatttgg ataaaagggt tacatttgca
    25081 gagcttcttt atgaaattaa taaaattgaa ggtattgagc gcatacgctt tatgacatcc
    25141 cacccgaaag atttaacgga tgatttgata aaagcaatta aagaatgtaa taaggtatgc
    25201 aaccacgttc atttgcctgt tcaatcggga agcaatgatg tgctaaatcg catgaaccgc
    25261 aaatacacta gggagcatta tttaaacttg gttgaaaaat taaaaacagc tgtacctgac
    25321 attgcaataa caacagatat aattgtagga ttcccgggag aaacagagga agatttcttg
    25381 gatactatag atattgttaa aaaggtcgag tatgattctg cttttacttt tctttattcg
    25441 ataagagagg gtacgaaggc agccaaaatg gagaaccaag tgccggataa tgtgaaacat
    25501 gaacgttttg aaaggctttt ggaagttttg tacccgataa tactggataa gaataaacag
    25561 tgtatcggta aagtatttcc cgtattggtt gaatctttcg agaacggtaa attaacaggc
    25621 aggactgagc atttcagatt agttcatttc aatggaagca aggatttaat cggtaaaatt
    25681 gtaaatgtaa aaataacgaa tgtaaaaact ttccacactg agggagaaat agtcgagtaa
    25741 taaggtatta atagacaatg gtagaagggg attgtaaatg gcaaagtata cacctatgat
    25801 ggagcaatat cttgcaatta aagagcaata taaagactgt attcttttgt acaggcttgg
    25861 agatttttat gaaatgtttt ttgatgatgc tattacagct tcaagggtat tagagattgc
    25921 acttacggga agagattgcg gacaagaaga aagggctccg atgtgcggtg tcccgcatca
    25981 tgccgttgac ggatatattg caaagcttat agaaaacggc tacaaggttg caatatgcga
    26041 gcagcttgaa gacccttcgg atgccaaagg aattgtaaaa cgcgatgtag taagaataat
    26101 aactcccgga acaataatcg agcaaggtat gctggatgaa aaaagcaaca attatttgtg
    26161 cactatatat ttggacaagg gttttggtat gtcttacgtg gacatttcaa cgggagagct
    26221 ctatgtaact gaaaacatat cgttaaatga ttttttgaaa aatagcactc acgatttatt
    26281 aatagaagaa atcaacaaaa taaccccgtc agaaattata tccaacaacc atatcggtaa
    26341 tgaagttata gattcaatga tatatttaag caatgtattg agcttagatg actataagca
    26401 aatagttttg aagcatttta aggtactatc attggactca ttagggctta aagagaatta
    26461 ccatgcggta atatctcttg gaatgctttt aaattattta aatgaaactc aaaaaacttc
    26521 actcgaccac attaataagc ttcattttta tacggtgggg gaatacctgc agattgatgc
    26581 aaacacaaga aaaaatttgg agcttattga aactattaga gggaaaaaag gccccggaac
    26641 actctaccat gttgttgaca acaccatgac tgccatgggg ggaaggcttc ttaaaaaatg
    26701 gattgaagag cctcttaaaa atattaagca tatttattta agattggatg cagttgaaga
    26761 attatatgaa aatataatga cttctaataa tattaaagaa tatttaaaaa gtgtttatga
    26821 tatagaaagg cttataagcc gaattgtata cggctcttgt aacggaagag atttaaattc
    26881 tttaaagcaa tccctctcaa atttaccgga tttaaaatca gaattaaatg agctttcttc
    26941 tcaaatgttt gccgatattt attcaagttt tgatgagctt aaggatattt ttgatttaat
    27001 tgatgcgtcg attgtagaag agcctcccat atcaattaaa gagggaggaa taataaaaac
    27061 aggctataat gaagagttgg atgaattaag ggaaattacg gtaaatggta aggattggat
    27121 ttccaacctt caagcaaaag aaagagaagc aaccggtatt aagaatttaa aaatcggttt
    27181 taacaaggtt ttcggctact atttggaggt tacaaaatca tatttaaaag atgttcccga
    27241 taattacata cgaaagcaga ctttagccaa ctgcgaaaga tatgtaaccc ccgaacttaa
    27301 agaaatggaa gctaaaattt tgaatgccga tgaacaaata atgaaactgg aatatgaatt
    27361 gttcttggaa ataagagagt tcataagcag gcatgtgaaa agaattcaag aaacagcata
    27421 taatattgca gtaatagatg caattaattc acttgcaatt gctgctgtta aaaataatta
    27481 tgtgcggcct gaaatgaaca agaaaggttt tataaaaatt accgacggaa gacatcctgt
    27541 tattgaaaga attatgaaaa acgagatgtt tgttccaaat gacacataca tagataacaa
    27601 ggaataccgc atgtcaatta tcacaggtcc gaatatggca ggcaaatcca cttatatgag
    27661 gcaggttgca ttaattgcac taatgtctca tataggcagt tttgttcccg ctaaaaaggc
    27721 ggaaatctgc ataattgata aaatatttac tcgtgtagga gcatcggatg acctctcaca
    27781 gggacaaagt accttcatgg tagaaatgag cgaggtttca aatattttga agaatgctac
    27841 ttctgacagc cttttgatat tggatgaaat cggaagaggt acaagcacct atgacgggtt
    27901 aagcatcgct tggagtgtag tagaatatat tacgaagaag ctcaaggcaa aaacattgtt
    27961 tgcaactcat tatcacgaat tgtccgagct tgagagcaag cttaagagcg ttaaaaacta
    28021 taaaatatta attaaagaag aacatgatga aataacattc ttaagaaaaa taactgaagg
    28081 cagcattgac agaagttatg gaatacaagt tgcaaatttg gcaaatcttc ctgaagaagt
    28141 aattgaaaga gcaaaagaaa tattaattca gcttgaagaa agcgatataa acaagccttt
    28201 tgctaggaaa aaaaacaggg tgaacgataa tttccaagtt tccatgttcc aggctaaccc
    28261 tgcagagaat gaaataaaca agaaatacaa agagctggca gaaaatatta agaatataga
    28321 tattgaaaat ataacaccta tgcaatcatt taaattgctt aatgatataa tagaaaaaac
    28381 taaacggata gatacataac gaaaggaatg tcttacatgg ctataataaa tttattggac
    28441 aatgaaacca ttaataaaat agctgccgga gaggttattg aaaacccaaa gtctatagta
    28501 aaggaattag tcgaaaattc aattgatgcc ggtgccgatg aaataacgat tgaaattaaa
    28561 aacggcggca aaagctttat acgagtaacc gataacggga agggaattga aaaagaccag
    28621 gttgaaaatg cctttaaaag gcattgtacc agcaagattt cttcttcaga cgatttgaat
    28681 tcgcttttta ctctgggatt tagaggtgaa gccttggcaa gcattgctgc cgtgtccaag
    28741 gttgaaatta taacaaggac aaatgatgat atctatggca ttaaaatgac tattgaaggc
    28801 ggaaaaatca tttctaagga agatatcggc tgccctgtcg gaacaacaat aactgtaaca
    28861 gacctttttt acaatgtgcc tgccaggaag aaattcttaa aaagcgatat ttccgaaagc
    28921 tcaaatatca atgaaattgt tgtatccctg gctcttagca aggagaatat ttcatttaaa
    28981 tatataaaca ataataatgt tgcctacaga actccaaaga caaataatct gctaaatact
    29041 atttcaagtc tttatgaaaa agatttatac aattctcttt taaagattga ctataaaggc
    29101 gatgttttag gtattacggg atatacaaca aatttaaact attacagggg taatagaaag
    29161 cagcagattg tttttgttaa cggaagggtt ataaagcata gaaggatgag ttatttcatt
    29221 gaagaagcat ataacactct tcttcccaaa aataaatatc cggcatgctt tattaaaatt
    29281 gaaatagctc ctgcattggt tgatgtaaat gttcatcctg caaagacgga agtgcgcttt
    29341 caaaatgaag aaatggtatt aaatgaaata aaaaatgctg tttattctgc cttgcactcc
    29401 aagagtatta tgaaggaagt aaaaggggaa accctcttac atgattcaaa agatataaat
    29461 aacttaagaa tatttgatag aagttcaggt aaatctgtca tcttaggcgg tttagaaaac
    29521 aaattcacgg aagaaacaga agaaacaaag aatattagga ttacggaagt tgaagtttca
    29581 aataaagata ttgccgtaat ggagtcgctt aaaccttatg ataaatttga atttaagcag
    29641 caaacattca taaaggcaga aaatgatgaa gataagctgc ctgagttatt cgttataggc
    29701 atagtgatgg atacatatat aatttgcgaa aacccggaaa aaacatgtat attcatgata
    29761 gaccagcatg cggcacatga aagaataaac tacgagaaat ttttaaatca atataattca
    29821 agagaaatct taatacagga aatagtaccg gaagtgatta atttatccta tgatgattac
    29881 gagacagcaa tgtcgaatat agaaatattt gaatatttgg gaatttccgt tgaaaacttt
    29941 ggaaataatg ctatactgat aaataacata ccaactatat ttaaggattc aaatttgaag
    30001 gatgtatttt atgcaatcct ggataaccta aaagatataa ctaaatcaaa tttagatttt
    30061 gttatggaaa aaataataaa agaggcatgc gtcaagtccg tgaagtccgg agacaggctt
    30121 catatgtctg aagtaagagc attgttaaat gaccttagaa tgacagataa cccttacact
    30181 tgtcctcacg gaaggcctgt aattattaaa atgaccaggt acgaaattga aaaaatgttt
    30241 gaaagaattc aaagttagga aagcaaaatg aataaaataa ttgtaattgt aggtcctacg
    30301 gctgtcggca aaacatatat atccgtagag cttgcaaaaa agttaaatac tgagattata
    30361 tcagccgatt caatgcagat atataaaggg atgaatattg gaactgcaaa aatcaccgag
    30421 gaagaaaaac aaggtatcat tcatcatatg atagatatta taaatcctga tgaagagtat
    30481 tcggtgtcag aatttaaata tgatgccgaa aaaattattg acaggctttt gagtgaaaac
    30541 agaattccca taattgtagg ggggagcggc ctgtatgtaa attctttgat atatgattta
    30601 gactttggca ataccaagtc caataaaaaa ttaagagaat actacactta ttattataag
    30661 gaacatgggg aagatgctct gtatgacaaa ttactgaaaa ttgaccctgt tgctgctgaa
    30721 aaaattcata aaaacaatat aaagagaatt attagagccc tggaagtgta tgatattaca
    30781 ggagttaagt tttcagagct aaacacggac ataagaaaga aaagcaataa atatgactgt
    30841 atattgattg gtctttcaat ggaaagaaaa gtcctttatg aaagaattaa tcaaagagtt
    30901 gatgaaatgc tttctaaggg tcttgttgat gaggttagct cacttattaa aaagggatac
    30961 ggtaagaatt tagtatccat gaggggaatt ggatataaag aaattattga atatcttgaa
    31021 ggtaatactg attatgagga agcagtcaat actttaaaac aaaatacaag aagatttgca
    31081 aaaaggcagt atacttggtt cctgaaagat gaaaacgtca aatggttcag catggataac
    31141 ccaagcgaaa ttgacaaaac tttggatagt atagttgaat atataagcaa gaaaattgat
    31201 agtattagta accaatgaga taaataataa taaaaatata cgctttcgct taattttatt
    31261 attatagtaa tgaagtaata acatatatgc aaatttatag gagaagggaa tgaatattat
    31321 taatttacaa gatacttttt taaacaaagt aagaaaagaa aaaataacaa taacgcttta
    31381 tttaaacaac ggtttcaagc tgacgggaac tgtcttaggt tttgataatt atgtaatttt
    31441 tatcgaaaca gataaaggtc agcaactggt ttacaagcat gcaataacat caattttgcc
    31501 gtttaaacag gtacgactgt tttttgacga gccagaggag caataatgtt aacagaaaat
    31561 aaattatgta aacagtttgg cataagcgaa gctgtttata attatgtaac aaaaattgaa
    31621 aaagaaatta agaagaatca ttttgaaaaa atagataaaa ttagagagct taatcaatat
    31681 aaagttatcc atgcaatgca ggagaacaag cttgattata caaactttta ctggaataca
    31741 ggctacggtt atggagatgt cggaagagaa aaggttgaaa aaatatatgc atccgtgttt
    31801 aagaccgaag atgcattggt aagaccttcc atagcttcag gaacccatgc cctgtacttg
    31861 acattgtcag gcatattgca gcacggtgat gaagtaattt ccatatccgg acgaccatac
    31921 gatactttgc ttacagtatt gggcgtgcaa ggaaaggaac ctaataatct cagtgaagca
    31981 ggaataattt ataaggaaat tcctctttat aaaaatgata tcgactggga aagtgcaata
    32041 aaagaagttt caatgaaaac aaagctttta atgattcagc gctcaacagg ctacagcttc
    32101 cgccctgcgc ttacaatcga aaaaataaaa aatgcaatag aaagaattcg ccaagtatat
    32161 ccaaatatat atattatggt agataattgc tacggagaat ttgtagacga aatggagcct
    32221 tcggatgtag gggcagatgt agttgtaggc tcgcttataa aaaatcccgg agggggagtt
    32281 gcattatcgg gaggctatgt ggttggaaaa aaagtattaa ttgacagaat agccaaccgt
    32341 cttacagctc cgggagtcgg aaaagagata ggacttacat tcggaaccac aagaaacacg
    32401 cttcaaggtt tgtttttcgc accacacatt gtgagcgaat cattaaaagg cgcactcttg
    32461 ttcggcgggg tttactcttc tctcggattt gaaataactc ctacagtaga agaggacaga
    32521 agcgatatta tacaggcaat taaatttaac gacaaaaaca tggtaattaa aatttgcgag
    32581 gctgttcaag ctgcatctcc ggtggacggt tatgtgtctc caataccatg ggatatgccc
    32641 ggctatacaa atcaggtaat aatggcttcg ggagcatttg tttccggctc atccatagag
    32701 cttagcgcag atgcaccaat gagacctcct tatatagcat atattcaggg cggcttgttt
    32761 tacgaccatg tcaagttggg agtaatgcta tcacttgaaa agatgatgga atttaatgag
    32821 ataaaaaata aaatattcaa ttaaaaatat ggggacattc cttatactac aaagactatc
    32881 tttatagtaa aggcgtgtcc ccatacctat gacattaata ttttctgaca acagctttaa
    32941 caacacccaa tatatagggc tcctttatta aaatcgggtc catggaactg ttttgaggct
    33001 gcaatcgaat gtggtctttt tctttataga aggttttaac tgttgcttca tctccgatta
    33061 atgcaacaac tatatctcca tttttagcat tgcgctggga attaactatt acataatcac
    33121 cgtgcaaaat tcctgcctca atcatactgt ctccctttac ttttaaaaca tatgattcct
    33181 tattacctac aaaatcaatc gaaataggca gggtgtcatc aatgttttca acagctaata
    33241 taggagagcc ggcagttacg gttcctataa tcggtaagtt tataatttct ttttttggca
    33301 tgtcgcagca tatatcatca agatcaatta attccaaagc ccttcttttg tttgttcctt
    33361 tttttatgta tcctttttct tctagtgtat ttaaatgggc atgaacggaa gaagtagagt
    33421 ttaaacctac agctttgcaa atttcacgaa tagacggggg ataacccctt aaagttaact
    33481 catcttttat atattgcaat attttaactt gtttatcgct taagccttca taactcataa
    33541 ttccacctct ttataaaatt attttagact cccgggagta cctattcgct tcgtccggat
    33601 agaacctatc tgaagcatcg atttaagctg tctgattgag tacttttcta tattttaaca
    33661 tagtttgaca agtaaagcaa acatttgttt gaaaaaatgt aaaaaattgt tgacatcgaa
    33721 ctaatgttct gttagaatga aaacagaaaa tatgttcgag gtgccttatg ataatattaa
    33781 ataaatataa agtaagtgat tcgaaaaaat ttaaaagatt tatgttcctg tccattttgc
    33841 ttataagtat tattactatt acctcaatta ctacaataaa agcatattca aaagatatac
    33901 ctcaatatga ttacgtaaga gttcaacacg gagatacctt atggtcaata gcaaaagatt
    33961 atgccggcag taaagatata agacaagtag tatacgaaat atccgagctt aacaagatac
    34021 ataataacct gattcatatc ggcgacataa ttaaaattcc cctaaattaa acttaaaggc
    34081 tctcatcatc attcatatca tttaagggat tttgttttac cgcatcacgg agcaattcct
    34141 tgtttatatg agtgtaaatt tgcgttgttg aaatactttc gtgtcccagt atttcttgca
    34201 gcgctctaat atctacatgg ccatattgat acataagggt tgctgccgta tggcgaagct
    34261 tgtgagggga gtatcctttt cctgataaac ctgcttcagt taagtattta tccaacatat
    34321 gctgaatgcc tttttgagaa aaacgggttt ttcgattact tataaaaaga gctttttcat
    34381 gacctttttt cggctcttgt cttacctcca agtaatcttc tacagctttt acacatacat
    34441 cattcaggta aattgttctt tccttgttgc cttttccgat tacagtcaag gtgtctccct
    34501 taattttatc gatatcgatg cttataagct ctgataatcg caggccgcaa ttcaaaaata
    34561 acataataat ggcatagtcg cgtttttcat tggagcctga tattgatgat aataaacttc
    34621 ttgattcatc cagcgttaat gctaccggca gacggctttt gttttttgga gtttctaatt
    34681 caagagccgg attttcaggt ataagcttta ctatggtatg taaatattta aagtatgacc
    34741 taagtgctga catttttcga gcttttgtac ggtttgaatt ttttctttcc ctgtccgtat
    34801 aggcaataaa agaatgaagg tccataattt ttactttttt tataatatcc aaggtaacag
    34861 gggagatatc gatttcttca aaattttcaa catcaggact taatttgaat ttgattacta
    34921 gaaatctata aaaggtacga aggtcaagaa agtattgact aacagtttta tccgctctgc
    34981 ctttaatagc aattgagtat tctaaaaaat cactaagtgg ggaagggcat aggtcattat
    35041 aatttatgta catcattatc tcctttacaa aaatgtcgca aaattcttac ttaattatat
    35101 agaagaatta ggaaaaagac aagctttttt agtaaaacct atatcatttt gattaataaa
    35161 ataacacaaa aatacttttc ttaaatgcaa gtgctcaaat ttacaagtat tgtgtgaatt
    35221 gcaattaact tttttcgata cttcgacata taaccagtat ttattcatat attcgacccg
    35281 tatatctgct tttaataaaa aataaccaca cattaattga aattttgagc atctcattaa
    35341 acttagtatt ctctgatgag cattagagga aagtccccaa ttcggacaag tcttagaggt
    35401 atgaggaata tatctgtctt atagatgata gaaatcaaat tttgcctgtt aattaaaaac
    35461 gagctatatt taaagggtat aaatggtcaa ggaaagaagt ttcgtgcgtt aaatcgcatt
    35521 ttaaagcctc gttttttggg catatctttg aaatttcagc cggttacttg acttggtcgt
    35581 tttttgtcta tagtgtgcaa gagtgaacga atatttttat tttttcatct tgagcatagc
    35641 gtagtaacta ttaacattgt cattataaga ataagcgtag tatctaatac ataagagaaa
    35701 gatccggtga gattttcgag ctacaacaat caggacagaa ctgtaaagaa aaaaatatcc
    35761 aaaccgcaaa ggtaaattat taaaaaatat tacaagaatt ataaatttgt gaattgaaat
    35821 tataaaagaa aaataatcaa atcgataaaa aaatcgagaa gaacgttgaa attccaaaaa
    35881 gaaggggtct ttcggaaaag tgcttatttc agggatatta tgtcgctaaa ttagcattta
    35941 gcgacataat aaaaaataat aatatttata taaattaaca attttgtaat tattactgct
    36001 attcccttga tatcccggtc aacgagaagc tctcaatgat actccgtcca aatataagtc
    36061 tgtatcatta ttgcttgcgt tgatagtatt aatgctgaca gtgaatctta ctattacgaa
    36121 atttacattt tgaggtacaa ttatgtctga cgttttttca tgataagaaa atgctgatga
    36181 ttctactgta acagctcttg gagctatttt ttctatttta attaattcaa cttcattaga
    36241 acctgaaata taaaaaactt cagcactaaa atataattct atatctaccg gtttttctgc
    36301 taatctttct gcaaagctta gcgttaaata acatccggga ttaacactaa ctgtttgtgt
    36361 taatgttgct ctgtctgttg tattcccggg taccggacca atatcaaaat tggcgctttg
    36421 tctgccttca tatgttatag aagtacttgg tcgtatattt atatcaagac cttccgtttc
    36481 gtcattccaa ttagcaaaaa ctgtttgaat tcctacttct tcaaagccac catttcttat
    36541 aagctcttca ggagtacatg aatttcctgt tgctaaatta ttgctgtttt tcgtatctgt
    36601 gcaagtattt atattattta ttacattagg attatctcta tattcccttt tcttacagcc
    36661 tgaatctttc ataatgttaa cgcctccaat atataaaaca agcttaagct cataaatata
    36721 atatgtacaa cctttataat tgttacataa ataaatgtgg cataaattgt attaaatgac
    36781 ggagaatcaa aaaaacatcg gagctcttca gccctgatgt tttgttatta tctgttatta
    36841 tttgaattct accttgacat tccccagttc aggaaaaaca cattcaaaca tatcaccttt
    36901 aaatgcttcc ggtgatgctg taattgctcc cgataaaatt atttcgccct ttttaagagt
    36961 aattccgtat tcacacagtt tatttgcaag ccatgcaaca cttattgccg gattccctaa
    37021 tacatcctta cctgttccgc tgtttaccac ttccccgttt ttataaagag tcatccttat
    37081 atcaggcaat gatacatcgt ctatttttac ttttttatcg tttaatatat acaaagcgct
    37141 tgaaccgttg tctgcaatag tatctaccag tttaattttc caatctttaa ttctcgaatc
    37201 cactatttca atggaagcac atatataatc agttgcttta taaacatcct ccacagttat
    37261 accgggaccc tttaaatctt ctttcagtac aaaagcaact tcaccctcta cttttggcct
    37321 tattaacgtt tttgtgtcta ctgtaccgcc gttttttact tccgtgcttt tatataaatg
    37381 gccgtaatcc ggctcggtaa tacccagcag ttcctgcatg ccctttgaag taaggccgat
    37441 tttcttgcca gttacaattt ccccgtcttc caatgctctc ttaacattta acagctgaat
    37501 atcataagcg tcctcaacgg ttacttcatt atcgatatca cttataacat ctataggttt
    37561 ctgaattttt aatgcttcgt aaagcatttt ggataattct tcgtttgtaa aatttctaga
    37621 catattttcc tccttatata ttaatatatt ctaatgtcca actaatcccc cgtccccttg
    37681 tcaccaaagg gctatgtgtc catctgctct cttttctgtt ccgccgcaga gagtgccttc
    37741 ttttgtcctt agtatcattt ggcctcgtcc gaaagatgat tcatttaact gtatttttac
    37801 atcatggccc ctgttaatca gctcatttat aatatatttc ggaacatcct gctctacttc
    37861 aacagtcatt cctttaatcc actgccacct cggtgcatct atggcttcct gaggattcat
    37921 atgaaaatcg attaaattac ttactacctg cacatgtcct tgaggctgca taaatgcccc
    37981 cattacacca aagggaccta gagcttttcc gtccttcatt aagaatccgg gtattatcgt
    38041 atgataggtt ttcttatgag gcattaaaca attaatgtgt tggtttacat aattaaaatt
    38101 atgtaaccta ttgttaagag ctattcctgt ttcaggtata acaatccccg acccaaatcc
    38161 ggtataattg ctttgaatga aagaaaccat attcccttcc ccatcagctg tacacagata
    38221 aacggttccg ctgctgtttg ggtcggtttc ttttaaattc aatgcattat cagtaattaa
    38281 acttccctta tctctgccgt aatcatccga taataattgc tttatgtctg ctttcatgta
    38341 tcttatatcg gtgatatact tctgaccatc atcaaagcta agtttaatag cttcaatttg
    38401 cttgtgatag gtatcgctct tatccttgta tgtaaaatca aaatttttta atatgttaag
    38461 cgccatcaaa gctaccagtc cctgaccgtt gggaggtatt tcatatacat cgtatcccct
    38521 gtaatttacg cttataggct ctacccaatc tgttttataa tcttctaagt cttcttttct
    38581 taaatacccg tcatactttt tgaaaaagga atctatttta tctgctatgg cacctttata
    38641 gtatgagttg ccgtgtgttt ttcctattga ttccaatgtt tcggcatgat acggaagctt
    38701 ccaaagctct ccgtaatttg gtgttctccc cttataggtg aatgtatcaa accaatattt
    38761 aaactcttct ttattttctt ttttgtaaat tttatatgca ttatcccatg ctttttttac
    38821 agtaacagaa acgggaaatc catacttggc ataattaatg gcaggctgta agacttctat
    38881 taaagaaagt ttcccgaatt tttgagataa ttctacccag gctgccggag caccgggtac
    38941 cattatcggt ataatgccgt actttggaat ttcagtaaat cctctttctt tcaatttttc
    39001 aattgaaatg ctgtagggtg aatacccgct gccatttaaa ccatgaagct tattattaaa
    39061 ccatacaagg gcaaatgcat cgcttcccaa accgttcgat gtaggctcaa caattgtaag
    39121 gcatgctgct gcagctatag ccgcatcaac ggcattgccg ccgtttttaa gtatttcaag
    39181 gcctgcttgt gctgctaatg gctgtgatgt ggcaaccatt ccgttatatc cgtagacaac
    39241 ctttcttctt gacggatatt tatattcggc agggttaaaa ttcatgtttt ttttactcct
    39301 ttctttgatt tatcatgaca acactcccta aggatgctta gaaattgtct gttattaaaa
    39361 agaagtaaac aaattgttta cttccatatt tttctaacct ttactttgca tattctgtag
    39421 ctctgatttc tctaattacg ttaactttaa tctgtccggg atattccatt tcttcttcta
    39481 tctttttaac tatatccttt gctattaaaa ctatgctcgc atcgcttgtt acttcaggtt
    39541 taaccattat tcttacttct cttcccgcct gtattgcaaa agacttttct accccttcaa
    39601 atgaatttga aatttcttct aacttttcaa gcctctttat ataagtctct aatgtttctc
    39661 ttcttgcacc cggtcttgca gctgatatag catctgctgc ttggactaag actgcttcta
    39721 tggtctgtgg ctctgtatcc ccgtgatgag ctgctatagc atgaactacg gccttgcttt
    39781 ctttgtactt tttagctaag tcttcaccta ttgtaacgtg gggaccttca acttcatgat
    39841 ccactgcctt tcctatatca tgcaataatc ctgctcgttt tgctactttt acgtcagcgc
    39901 ctaattcagc agccataata cctgctaaat atgatacttc aatggagtgt ttcaatacgt
    39961 tttggccgta acttgttctg aatttcagtc tgccaagtaa ttttaccagt tccgggtgaa
    40021 gcccgtggat tccggtatca agggttgcct gttcgccttc atccttaatt atttggtcta
    40081 cttctttttt tgccttatca accatctctt caattcttgc aggatgtatc ctaccatccg
    40141 caattaactt ttcaagtgct aatctggcaa cttctcttct aatagggtca aatccggata
    40201 taactactgc ttccggtgta tcatctatta taatatctat acctgtaagt tgttccaagg
    40261 ctcttatatt tctgccttcc cttcctatta ttctgccttt catttcgtca ttcggcaggt
    40321 cgactacaga tacagttgtt tctgctacat ggtctgcaga acatttctga atcgcatatg
    40381 taataatttc acgtgctttt ctttctgcag tttcttttga ttccttctcg atttctttga
    40441 cagcaaccgc ggcttcctgt tcagcttctt tttttatgtt gttgattaga atgcttttag
    40501 cttcttctgt tgaaagtccg gatattttct cgagttcctc aatttgtttt tggtgaattg
    40561 catctaattt ttcctgcgtt atttcaattt cttttaactt cttttggagc aattcgtctt
    40621 ttttctctac tacaaggctt ttagagtcca gcatttcttc ttttttaatc agcctttttt
    40681 cagcccttaa aatttcgttt cgtctttctt tgttttctct ttcaacttca tttcttaacc
    40741 tgtgagcttc gtcttttgct tcaacaagta tttctttttt tcttgtttcg gctttttgat
    40801 ttgcgtcttc gattattctt tttgcttcta attcagcact acctattttg ctttcagata
    40861 tatttttacg aataaaaaat cctataatta tacctattat tgcgcagata ataccgacta
    40921 ttatttcttc gaacaattca gcacctccaa tattaaattt tcaagctgcg aaatatatac
    40981 atttttccta attgctataa aaaatgtgta tcttcaaaaa catatatttt aatcgtctta
    41041 attagttttg taacctgaat ataatttatt ttataattat ttataaacaa tgtcaagata
    41101 taatgtttat atttgcatat ttttacatat tctaatgcat atcaaagcgt gttggtttac
    41161 ataatttaaa ttatgtaaac tatggtatat agtttgtggt atataataaa aaggcactta
    41221 tagtgcctaa gcgaaagttc tcaaagttat tcatatgata atgggtcatt ggcatcagat
    41281 gctactttta aatctcccag ttcgtacttt tcaagtacct ttttctctat ttcatccata
    41341 agctccggat ttgacaagag gtagtctttt gaattttctc tgccttgtcc gattctcgta
    41401 ttttcgtagg aataccatgc tccggcttta tttacaactc cggcttcaac agccatatca
    41461 agaatgcatc cttcttttga tatacctttg ccgtatataa tatcaaattc agcttgctta
    41521 aaaggcggtg caactttgtt tttaacaact ttaactctgg ttctgttgcc gtaaacatca
    41581 tcaccctttt tcaaggtttc tactcttctt acgtctaatc tcattgaagc ataaaatttt
    41641 aaagcacgtc cgccggttgt ggtttcagga ttgccaaaca ttactccaat tttttcacgc
    41701 agctggttga taaatatagc cactgcattt gatttgctta tagctcctgc taattttctt
    41761 aaagcctgcg acattaatct tgcctgcagt cccatgtgag aatctcccat ttctccgtct
    41821 atttcagctt ttggaactaa ggctgctaca gagtctacaa ctatgacatc tatggcaccg
    41881 cttctgacta aggcttcagc tatttcaagg gcttgctcac cggtatcagg ctgtgatata
    41941 atcaaatctt caaccgtaac gccgagagct tttgcataag cagggtcaag tgcatgctct
    42001 gcatcaataa atgcagctat tcctcccttt ttttgtgctt ctgctactat atgtaatgct
    42061 actgttgtct tacctgatga ttcaggtcca aatatttcaa taattctccc ttttggtact
    42121 ccgccgattc ccgttgctat atcgagaggc agtgcaccgg ttggtattgc ttctatattt
    42181 aatttcgcat tttctccaag cttcattata gaacctttac cgaattgctt ttctatttga
    42241 gcaatggcaa gctcaagtgc cttatctttt tccatatccg actcctttag aacatttgtt
    42301 cacctttgat tatacataga ataatatgta ttgtcaacaa tttaattttt tataacacca
    42361 atattttcat taatatatac tgctcccgaa gttatggtca agagtacggt tattgcaatt
    42421 aaaggagcta ttatagtatt tgtaaaacca aaatatcctg tatagtttaa taaaattgca
    42481 ataattaata ccatctggaa cactgttttt attttacccc atgcattggc agcaatagtg
    42541 acaccttttg atgcagccag tgttctgaaa ccggaaacca aaaattccct tgatataatc
    42601 aatacaacaa cccatgcact tatatatccg agctcaacaa aagctatgaa agcagaagcc
    42661 gtcaaaagct tgtctgcaag agggtccata aattttccaa agtcagatac taaattatct
    42721 ctccttgcaa tgtatccgtc gactaaatca gtaacagccg cgcctgcaaa gattaacagc
    42781 ggcagaaacc ttaaaattcc tttgcttatg tataagaata acagaaataa aggtattgaa
    42841 attactctta taacagtaag tttgtttggt aaatttaaat tcttaaacca atctactatc
    42901 ttcatcactc acatctccta tcaaatcata ttctaatgca tcattaattc tgactttata
    42961 aatttctccc aaccttaatt ctttatttga tgctacataa acacagccgt caatttcaat
    43021 cgaatccatg taagttctcc ccacatagac attttccgca tccttctcct caatcaatac
    43081 atcataggtc ttaccgattt tcaataaatt attttcgtaa gatatctctt gttggatttc
    43141 cattaactca tttagtcttt gctcttttac atcttcgtct atctgtcccg gaagcttgta
    43201 tgccggtgta tcttcttccc ttgaatattt aaaaatccca actcggtcaa gcctgtattc
    43261 tttcaaaaaa ttctttaatt cttcatgctc atcccaagtt tctcccggaa aaccggttat
    43321 taaagatgtt cgtataacgg catctttaac ttccactcgg attttttgta taagagatgt
    43381 aatttgttcc ctgcttgttt ttctgttcat tctttttagg attctgtcat tgatatgctg
    43441 cattggaata tcaaaatact taagaagctt tttattattc ttaaattcat ttataagctc
    43501 gtcatatata tcttccggat ataaataatg aattctaatc caccggattc cttctatttc
    43561 tgaaatcttt cgaataagct catgaagttt tttttcaccg taaatatcaa ttccgtactt
    43621 gctcgtgtcc tgagcaataa ttattatttc ttttacgccc ttgctggcaa gaactttcgc
    43681 ttcctcgacc aaattttcaa ttctgcggct tttaaaaagc cctctaagct tcggaatcac
    43741 gcaataggta caattattgc tgcagccttc agctattttt aaataggaaa cccaagcctc
    43801 agtcatagta tttcttggaa gctccttgtc aatatcaagc tccaatgata tatctataag
    43861 caattcttta ttcttttcgt attcattaat atattcgtca attctttcaa aaccggctgt
    43921 accaatgata atgtcaattt caggtatttc ttttgacagt tcttctccga atctttgaga
    43981 taggcagcct gtgactatta tttttgtttt tttgtttacc ttgtttttta tgtctgctgc
    44041 aaaaaggatt gcatttaccg actcctgctg tgcatcattt ataaaggagc atgtgtttat
    44101 tatgatataa tcggctttat ctgcataatc cgtaacctta tacccttttt tggaaagtat
    44161 gcccagcata ttttccgaat cgacaaggtt ttttgaacat cccaatgaat gcatgtagat
    44221 tttcttcatt tattatttaa tttcctctca ttttgtctaa ttcttctttt gtaataagga
    44281 ctttcctcgg tttacttcct tcatgtcccc ccacgatacc tctttcctca agctggtcaa
    44341 taattcgagc agctcttgaa taccctactc taaattttcg ttgaatgtag gataccgaag
    44401 cctggccatc atttactacc gtctcgattg cctgctccaa atattcatca acttcttcat
    44461 tatttgtttg gttttctatt tctttgatta tttcgttttg tttgaacatt tttatttcaa
    44521 tttgctgttc tttaatataa tcaacaactc tttttatttc gtcatccgat acatatgttc
    44581 cttgtattct tacgggcttt ggaaggccta tcgggtgata aagcatatcc cctcttccaa
    44641 ggagcttttc agctccgcct gtatccaata tagttcttga atcaatatgt gatgatacgg
    44701 caaatgcaat acgcgaaggt atatttgctt ttataactcc tgtgataaca tccacggaag
    44761 gtctttgcgt agcaataacg aggtgcatac cgcaagctct tgccagctga gctatacgtg
    44821 taatatattc ttcaacctct gccgatgcta cggacataag gtctgacaat tcatctataa
    44881 ttattacgat tttaggcatc ttctctttgt tttctgttac cattttttca ttgtatgagt
    44941 ctatatctct tacgctgctc tgagcgaaaa tcttgtagcg gtttgtcatt tccgaaattg
    45001 cccagttcag agcattggct gcttttctcg ggtcagttac cacaggtatc aataaatgcg
    45061 gaatgccgtt gtaattgctt agctcaacaa ctttcgggtc aattagtata agctttactt
    45121 catcgggttt tgctttaaat aaaatactca ttattagtga atttatgcat acgcttttac
    45181 ctgagcccgt agctccggct attagcaagt gaggcatttt atcgatactt gcaattatat
    45241 tcttgcctgc aatatccttt ccaagcgcaa atggaatttt tgtcggcgca tttctatact
    45301 ctcttgattc aatcatactt cttaaataca cgctggtttt tgctctgttg ggtacttcga
    45361 ttccaattgc cgctttgccg ggtatgggtg cttccatgcg tatttccgag gttgcgagag
    45421 ccagtgctat atcatttgat aatgctgtta tgcggcttac ttttattccc ggagcaggtt
    45481 gaatttcata cctggtaata gtcggaccct tgtttatttg aagtatatta catggtatat
    45541 taaagttgtt taatgtttca ataagctttt caccgtcttt ttttaaatct tccttatcac
    45601 ccctaatatg aagcggcata gggtctaata gcttaataga cggcaacttg taattgtctg
    45661 ttttaacatc cgatattgta ataatttcat cagttatttc tttttgcttg aatttttcct
    45721 tagattcttc aatcggcaca tcttttgatg cttcaggaat attttcgttt ttttgaatat
    45781 cttctatttt taattgagta atttcagctt ttttattttc ttgaacagca ctcatataat
    45841 cataaatttg tatgctttct tcttgctgtt ttttagcagg tttttctttt ttagtcttgc
    45901 tttcgtcact ttccgtaaat atgaatctat atataaggtt tgtaaatttt tgtatgtaat
    45961 ttttaatcaa tttcaatatg ccgattatac tttgatttgt aagaagaact atagatgaaa
    46021 agataagtgc gcccaaggct acatatgttc cgtttttacc gataagcttt acaaataaaa
    46081 atgtaatcgc atttccaaaa actcctccgc ctttgttacg cattccgaac tctgcagaag
    46141 ctttaatcaa ttcctgaaag tcgttgctgt cagccatatg agcatataca tccgcatcgg
    46201 atattgcctt gaagattatg aaacaaagaa aaaatccgat aattgcggct atttgattat
    46261 tatcattcaa tataataata tttaaaagtc tcaaaatccc tacagctaat ataaaatacg
    46321 gcaatacctt agcgcctgcc ccgaatgcgg aatgagtaaa ggtatttata cttttgccga
    46381 ttataccggt ttgggcttcc ttagttgcta cgataatcat gaaaacagca aatgatataa
    46441 taaaaatacc ggttatttct ttaattaaaa tatttttgcc ggatgcttta gagcttgtac
    46501 tctttttatt tatgcttgtt tttttatttg ttttattagt tttatttgtg gttgttttag
    46561 ccatttaaag caatttcccc ctgtttcttt gtaaatttaa ttatatcata ggatattatc
    46621 attccgcaat ggtaatttgt cgatatccta atgattgggg acatttctct ttttatttac
    46681 aatgtcccct tatttttaac cctcttcttc catattgtcg ataagctctc tcagcttttt
    46741 cattgctttg ctgaatccgc ctatttcgtc aatcaatcca atatttacaa cttcatcagc
    46801 gtttaatacc gttcctacat cattggctat ttcatccgta tcatacatat aatctaataa
    46861 ggcttgtttg tcaacatgag aatttgatac gataaaatta gtaatccttt cctgcatttt
    46921 ctggaaatac ctgaaagttt gaggtactcc tattactaag ccgctggtac gtatcggatg
    46981 aattgtcatc gtgcctgtag gtgcaatgaa agagtaatcg gcagaaactg ctaaggctgt
    47041 tccaatgctg tgtccccctc ctaatacaat tgaaaccgta ggtttcgtta tgcttgctat
    47101 caattctgaa atggcaagac cggcttcaac atctccaccc atcgtattaa tcagcaataa
    47161 aatacctttt atctgagggt ccatttcagc tcctattatc ataggaatta tatgctcata
    47221 ctttgttgtt ttgctttgag gaggcaatga agagtgtcct tcaatttctc cgattatact
    47281 tataaaacaa atatctttta ccggttccac ttgagttatg cttccaacct gtttaacatt
    47341 ttctataggc tggctgccat cttcattatc attaggtgta ccgctgaata taaaggggtt
    47401 tatcggctct gttgaggatt gagcttggtt aagtgatggc tgtgtcttct gttgcgggat
    47461 attcatcttg ttattttcca tagttcctcc ataaaaaaat aatcaggtat tcctgattat
    47521 tctttgaagt tttattaaaa atatacactg acatcatgat tgtcagtcat tttctatgat
    47581 aatcatgtcg cttccgattt tttttatatt tttccacagt atttcttttc tttttggttc
    47641 tttgaaaaat gcaaaaggtg aattgccgcc ggatataaag ctgattattt cgcctgtttt
    47701 ttcatctata accaagtcac agtcaccgaa aattcccaaa ttttctccgg tgtcaacatt
    47761 aataatttct ttgctgtata actcgcttaa tttcatatat tcccccccat ttctatttct
    47821 tattcaatta taatgttaat gaattcctgt atgaccgaaa cctccctgac ctctttctgt
    47881 ttcgtttaag tcttcaacta attcaaaatc tgccctttca tgcttcataa ttaccatttg
    47941 ggcaattctg tcaccgttgt ttatagtaaa atcttcgcca ccgagattaa ttaaaattac
    48001 ttttatttct cctctgtaat ccgagtcgat agttcctata ccgtttacaa ggcttatacc
    48061 gtgtttgatt gccaaaccgc ttcgagctct cacttgtgct tcgtaacctt cgggaagttc
    48121 aataaataac cctgtgggca ccaaggctct ttccatcggc tttaatatta agggtgcatc
    48181 aacataggcc tggaggtcta ctcccgcaga gccttttgtt tcatattcag gtaatttaaa
    48241 tggatttttc tttactactt taattttcat attcttctcc cagtacttta taaattttat
    48301 cttcaatatc agaatgttta tgtaaattcc ccgcaaatgt aatactcagg ttgtcaatat
    48361 tgaacaattc ttttgctaca tccaaaacat catcaatttt aacattgtta atgctttcaa
    48421 ttatttcatc aggatacagg atttcattat ataagagctc tcttctgccg atagctgaca
    48481 ttctgctcga tgtgctttca agactcaata taaaatttcc tttaatttgc tgctttgctc
    48541 tgttaaattc tgcttcttta atatttccgt ttttgatgct tgccatttcc tttagaattg
    48601 ttcttaaagc ttcttcgacc tgctcgtagc tcatggctgc ataaatgctg aaaatacctg
    48661 tatcggaata attggttaca aatgaataaa tagaatatac tagacctttt ttctctctta
    48721 cttcctgaaa aattcttgag ctcgccgttc cccccaacaa attgttcaat actaagagag
    48781 gatgataatt tttgtcatgc cttccaaatg ttttattgcc tatgcatatg tggagttgtt
    48841 ccaaatcctt attaataccc cttatttttc tttggtaatc tgagttttta ttaagacgtt
    48901 ttacatcttt attttcccaa tgtttaaatt tttcctcaat cagcttaatg aattcatcct
    48961 catcaaaact tccggcaatt gaaattacag tattagacgg agaataattt tcttccatat
    49021 atttttttac tttatcacta gtaaaagttt ttaaattttt ttcggtacct agaatattga
    49081 atgccatagg gctgtcgtta aaaattattt cagataattt atcgtataca atatcctcgg
    49141 gcgaatcttc atacatttta atttcttcaa taacaactga tatttctttt tctatttcct
    49201 caggtgaaaa caaagagtta aaaaacatat ccgatagtat atccaatgct tcgtttatat
    49261 tttcttccaa aacttttacg taaaagctgg tgcattcctt gcttgtgtat gcattcatct
    49321 gagcgcctaa attgtcaatt tcctctgcaa tttcatttgc ttttctattt tttgtgccct
    49381 tgaaaagcat gtgctcaata aagtgagaca atccgtttgt ttcttcattt tcattataag
    49441 aaccgacatt aacccatata ccgatggatg cagactttac gtagtcgatt ttttccatta
    49501 caatccttaa gccgttatct aatgtgtgtt tttttatcat ttttcctccg cttactacat
    49561 gctaatgctt atatttcttc atggactcac ttcatagtct tacccgtcat tatggtgcgt
    49621 atctctttca caatgcatta gggtgtttcc ccatacctct ttacctacca ttttaactct
    49681 catagtcctg aatctgccta ttataagtaa aattattata attaccagaa aaattataat
    49741 caataatttt cgtatgaaag caacattgca aaaaaatttc ataaaaatcc ccctctatac
    49801 ttaaatgtat tattcatgag gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49921 natatgatat ttgttatctt aagtgtttta ttatttttgt acttgctgca ttgctgcttt
    49981 tctggaaagt ttgattttgc ctttttcgtc gatgccgatt acttttacta atacttcgtc
    50041 acctattttc aaaacatctt caaccttgtc aacccttttg ttgtcaattt gtgaaatatg
    50101 aagtaatcca tccttattat cagaaagttc gacaaaagct ccatagggct ctattcttac
    50161 aacttttcct aagattattt cgtctacttc gatttcttta acaattcctt ctattattct
    50221 cttagccttg tatgcatttt caacattttc ggctgctatc aaaacggagc cgtcatcttc
    50281 aatgtcaatt ttaactccgg tttcttcaat aatcttgttg attacttttc ctcccgagcc
    50341 tataacatct cttatttttt cagggtcaat gctcattgaa ataatcttcg gtgcatactt
    50401 gcttacttct tctctcggct tgtcaatgca ttcaagcatt ttatccagaa tgaacattct
    50461 tcctacataa gccttctgca aagcttttga taatatttcc ttatcgattc catgcacttt
    50521 tatatccatt tgaatcgctg taattccttc tcttgtacct gccaccttaa agtccatgtc
    50581 tccaaagaaa tcttccagac cttgaatatc agtaagaaca actacattgt ctccttcttt
    50641 cataagtccc attgcaactc cggctacagg cgctttgatt ggaactcccg catccatcaa
    50701 tgataatgtt gagccgcaca cgcttgcttg tgaagttgac ccgttagagc ttaaaacctc
    50761 ggaaacaagt cttatggtat aaggaaattc ttcttcactt ggcagaacag gctctaaggc
    50821 tctttccgcc aaagctccgt ggcctatttc tcttcttccc ggtcctcttg acggtcttgc
    50881 ttcaccgaca gaataggatg ggaaattgta atgatgcata taacgttttg aagtctcttc
    50941 ggatataccg tccaatgtct gctcgtcgct tgatactcca agtgtggcaa cagtaaggac
    51001 ctgtgtctgt ccccttgtaa aaagtcccga gccgtgtgtt ctaggaagta ttcctacttc
    51061 acaggatatt ttccttattt cgtctattcc cctgttatca ggcctgatac ctcttagaag
    51121 tatattttct cttatctttt ccttaactat tgtatacaga gcttcgtcaa tatccgtttc
    51181 attttcaggg tatttttcca agaatatttc tttagcttgt gctttaaagt tgtcgatatt
    51241 ttcttgtctt ttaagtttgt cttcttcaat aatcgcttcg tttattatgg gagcggcata
    51301 ttccctgatt tctttttcga tattttcatc gcatacaaag caggtataag ctgatttttc
    51361 tttaccaact tgggctgtaa tatcaattat aaaatcacaa agttttttta tttcttcatg
    51421 tgcaaacatt attgcatcaa gcattacttc ttctttgact tcatttgcac ctgcttcaac
    51481 catcatgact gcgtcttttg ttcctgatac agttaaatca agttctgtta tttctctttc
    51541 cttgctgttt gggttaacaa taaacttccc gtcaattaat cctatattta cagcacccgt
    51601 aggaccttca aacggtatat ctgatattga taatgcaatt gacgaaccaa tcatacctgc
    51661 aatttcaggt gagcagtcct ggtcaaccga caaaacagta acagtcactg caacatcatt
    51721 tctgtatcct tttgggaaaa gtggtctcaa aggtctgtca attaaacgtg aagtcaatat
    51781 tgccttttcc ccgggtcttc cctctctttt aataaatcca cccggtatct ttcctacaga
    51841 gtatagtttc tcctcaaaat ctacactgag cgggaagaag tcgatacctt ctctcggctc
    51901 ttttgaagct gccacatttg ccattattac agtgtctccg tattgaacaa tgcaagagcc
    51961 gtttgcaagt tcagcaattt tgccgattgt tacttttaat tcccttccgg caagtatgta
    52021 tttaaatatt ttttcctcca tgttacctcc tttaacgtaa aatataaagc gggttccccc
    52081 gctttaattt cacttacagt ataagcgttc gaaccaagtt gcaaattggg tcatctgctt
    52141 atcttcttaa tcctaatttt gctacgatgc ttctatatct ttcaatgtca ttgtctttta
    52201 agtagttcag aaaacctctt cttcgtccaa ccatttgtaa aagacctctt cttgaatgat
    52261 ggtccttttt attctccttt aaatgttcag tcagattgtt gattctgtaa gtcaacaaag
    52321 ctatttgaac ttcaggagaa ccggtgtctt tttcattaat tctgtactgc tcaataattt
    52381 gtgttttgat ttccttattc atttttacct ccatatattt atttcaccat aatccgagca
    52441 aatcgttgag gagtcgataa acttagacta tggaacttat aaagtctaac atatttattt
    52501 atttttgtaa acaacaattt tttaagaagc tttattt
//
LOCUS       DAOPUE010000003        51360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_610_length_51360_cov_10.586337, whole genome shotgun sequence.
ACCESSION   DAOPUE010000003 DAOPUE010000000
VERSION     DAOPUE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 51360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51360
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_610_length_51360_cov_10.586337"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="whiA"
                     /locus_tag="PKH42_00570"
     CDS             complement(<1..937)
                     /gene="whiA"
                     /locus_tag="PKH42_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOA18948.1"
                     /translation="MTFSSRVKDELSRTEISDVLCANAEAAAFVRTVGYITIKGLNKV
                     EFEFLTENAAVARRIFKVLKIAHNINADVSVSKSNRLKKQNNYIIKIDEKLTKQFLSD
                     SQIIKDNDFNVMNFNYEVPEELLASDLCRRAFIKGSFMGSGSISNPEKSYHAEFVSNK
                     EVQSKTLQKLLKIYGINGKNIYRKNNYVTYIKESEQISDLLALMGANNAVLDFENIRA
                     VKETRNQINRVINCETANLDKIVDTSMRQINNIKILKKYKVIDQLPDNLRELAYLRLK
                     HSNASLKELGQMLDPPLGKSGVNHRLRKIEEIAEDL"
     gene            complement(946..1371)
                     /locus_tag="PKH42_00575"
     CDS             complement(946..1371)
                     /locus_tag="PKH42_00575"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOA18949.1"
                     /translation="MVDEISAGGIVFFKNNILMLKKYNGDWVLPKGRVEENESLKETA
                     LREVYEETKAKVTPIKYLGKISYEFSRTCYLDKIHIRKEVHWYLMMARNMNCTAQKNE
                     GFIDAKFLPFERSLTIARYDDERKIIKKAVDDIKFHSYK"
     assembly_gap    1457..1556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1557..2686)
                     /locus_tag="PKH42_00580"
     CDS             complement(<1557..2686)
                     /locus_tag="PKH42_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOA18950.1"
                     /translation="MEKIKLAAISAFGLEAVVKRELTDLGYENIVTDNGWMYFDAEIK
                     DICKTNINLRCADRVMLVMGQFETLSFEELFDKTYELPWEKWITKDGKFTVNGKSVKS
                     KLYSTPDCQRMVKKAISKKLCEYYDTDWMPETGAEFTILVSIHKDIATISIDTTGARE
                     GLFKRGYREKSMAAPLKETMAAALVKLSYWNKNRVLYDPLCGSGTIAIEAALIGRNIA
                     PGLNRNFASMEWPLVKDEYWKNEKIEARKRIDFDSEIKIYASDVSEKAIRIAKENAIE
                     AGVDDCIEFFVKDVTHIEKPMCSYGVLITNPPYAERIGDEEQLTKIHKKLGQVFGRDK
                     TWSVYVITSSVNFEKEFGRKADRKRKLFNGDMRVDYYQYFGE"
     gene            complement(2758..4185)
                     /locus_tag="PKH42_00585"
     CDS             complement(2758..4185)
                     /locus_tag="PKH42_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA18951.1"
                     /translation="MNNEIEFSKQDREKFEYILDNIPDAITIVDNYGKVIFFNSTSEE
                     YFNLKKSDIIDKDLVEFFPTAILLRVLDSKTSFYNIYNSPRENSFVVSSAAPLYDRNG
                     NMIGALARDRDITEIVRLSELLNKTQSNLLKLEKEYSKVTSSGEAYFSKIISNNAAFI
                     EIINLCKNISKTPLNILLKGESGTGKELFAKAIHYESGRKGKFIPINCSAIPRELFES
                     EIFGYEAGAFTGAKSEGKKGKFEEAEGGTIFLDEIADLPLELQPKLLRILEDGVVTRI
                     GGNKPKTIDVRIISATNKDIRELINEDKFRKDLYYRLDIFQVNIPPLRERKGDIILLA
                     NRFLQEFCMEQGISIVEIPDEIMSIFSEYRWEGNVRELRNVIQRCAVIASQRGKNKIE
                     REYLPSYMQDIEVDEIKKLINLNEIKSYSGLEEYIQQIEKRIIIKTLEEMNYNKKETA
                     KKLQIPRATLYYKMDKYGISRDDKY"
     gene            complement(4267..5418)
                     /locus_tag="PKH42_00590"
     CDS             complement(4267..5418)
                     /locus_tag="PKH42_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOA18952.1"
                     /translation="MNIDEIIKNSLTENKFIYCIFTTPRVKSQYKKITVRPIAIKDRE
                     YLQLEKFTETKAFHENLDYEVALDVLLNMVNEYRNINLFTTDADYQILVSKKGSIKIT
                     RSEPVKKLKKEEHNKKKQYLISENQPCDFLIILGIMNKDGKVYTKKYDKFKQINKFLE
                     IVDDSLAGKSIKDGFTIIDFGCGKAYLTFALYYYFYYIRRIKVKITGLDLKEEVIGFC
                     NETAKKLNYENLNFVYGDISDFEYKNKVNMIVTLHACDTATDAALVKAIAWDCDIILS
                     VPCCQHELFNKIENENLNPMLKHGLIKERMSSLITDSLRGLFLETKGYKVQLMEFISM
                     EHTPKNILIRAVKSNKNTESAEIEYEKFKKYWNLNNLFIENYYETIINP"
     gene            complement(5434..7116)
                     /locus_tag="PKH42_00595"
     CDS             complement(5434..7116)
                     /locus_tag="PKH42_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOA18953.1"
                     /translation="MSEFNNENENRESVNFIQKIINEDLEKGTYGNRVHTRFPPEPNG
                     FLHVGHAKSICLNYGLAKKNNGIFNLRFDDTNPTKEDDIFIESIIKDVKWLCPDWEER
                     IFYASNYFGKMYDYAVELIKKGLAFVDDLSADEIREYRGTLTEPGKESPYRNRTVEEN
                     LELFEKMKNGVFKDGEKVLRAKIDMASPNINMRDPVIYRILHESHYNTGDEWCIYPMY
                     DFAHPLEDAIEGITHSICTLEFEDHRPFYDWVLSNIDDFKDAPTKQIEFAKLFLSKTM
                     MGKRYLKQMVEEGYVDGWDDPRMPTIAGFRRRGYTPEAIHNFCEAIGVSKAQSVVDIA
                     MLEFAIRDDLGPKVPRTMAVLDPIKLIITNYPEDKVEYVETENNPDNPDMGSRMIPFT
                     REVYIEREDFMENPPKKYFRLFPGNEVRLRNAYFVKCVDFVKDEDGNVTEVHCTYDPE
                     TKSGSGFTGRKVKGTLHWVSAKHCIDAEVRLYDYMMEDEEYDKTNFLEKLNPESKVVL
                     KNCKVEPSMKEFKVGDRFQFLRHGYFIIDQDTTEDNLVFNRIVSLKSSFKGV"
     gene            complement(7355..8209)
                     /locus_tag="PKH42_00600"
     CDS             complement(7355..8209)
                     /locus_tag="PKH42_00600"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOA18954.1"
                     /translation="MEIKKICIVGTGTMAKGIVQTFAQAGYPVIVKGRPGNTFDTLET
                     VIGKGLSKLVAKGKIEQSKMDEILGNITKTTTYEECADADLFIEAVAEDMVTKHAMFA
                     DIEAVAKEDAILSTNTSSLSITEIAAKSKNPGRIIGMHFFNPVPVMKLVEIIKGKLTD
                     DEVHAKVFELAQKIGKTPVSVEEAPGFVVNRVLIPLVNEGIGILADGVASAEEIDTAM
                     KLGANHPMGPLELGDLIGLDVCLAIMEVLYNEFGDPKYRPHPLLRKMVRANLLGRKTG
                     KGFYDYSK"
     gene            8371..9207
                     /locus_tag="PKH42_00605"
     CDS             8371..9207
                     /locus_tag="PKH42_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOA18955.1"
                     /translation="MIKKFLIFATKLINKTKEDDILALSAQLSYYLILSIFPFLILAI
                     SLMCGYSEYIYSILNSLSDVIPEEVYRIIYNVLKYSVASCSKPYLTISMLIIIWSATS
                     GSAAIINGINIAYGFNTKKNFLFLRIRGILFTLALMVSMQIIFAAVVAGRTLLLFVKG
                     ISIFEDYNFIFIDILRFAVTFFIVLIILLASYKFLPYEKVKFSYAYPGAIFAAAGSIA
                     GSYLYSRYVSTRVVFINSIYGNLSGLFVFIIWIYILSIIFLMGAEVNYFAAKQKNGIN
                     GK"
     gene            complement(9268..9873)
                     /locus_tag="PKH42_00610"
     CDS             complement(9268..9873)
                     /locus_tag="PKH42_00610"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA18956.1"
                     /translation="MEQYFTENPTTEKEIYKFEWNVGKEVLYFYTSNSVFSKKGVDFG
                     SMLLIETILNENEDFSGSILDLGCGYGPLGVIAAKKLKKSFVTMCDVNERALELSRMN
                     AKENKVDDRIKVIASSAFENIKDNYDIIMTNPPIRAGKNVVFSFYEGAYEHLNDGGKL
                     YVVIQKKQGAPSTKAKLESLFGNCETADKKSGYFIFRCIKK"
     gene            complement(9938..10756)
                     /locus_tag="PKH42_00615"
     CDS             complement(9938..10756)
                     /locus_tag="PKH42_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOA18957.1"
                     /translation="MPRQARIFSDTGVYHIMMRGNDKGRIFLDDDDRRRFISTLFEKA
                     SEENTDIYAYCLMSNHVHLLLHDKEYNITRLMKRINVSFAYHFNKKYKRVGHLFQDRF
                     VSEIIDNNDYLLAAVRYIHNNPVTAGIVSSPEKYLWSSYNDYISSKKSSSLTTDKILN
                     IFSDNITVAIKQFIDFSSLDCDIKFIEFIEKSNEELRMEQEKRATETLDNILLLKGLC
                     INDLSKKENHNLRNKLTIYLKEEFNLSSRQIAAIIRISKGTVLKIMKEMKIGNN"
     gene            complement(11006..11452)
                     /locus_tag="PKH42_00620"
     CDS             complement(11006..11452)
                     /locus_tag="PKH42_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HOA18958.1"
                     /translation="MNFVEYEKECEKIRNDNKKYLEIFQSEMIEKGLSEKTIQSHLNN
                     IDFYINDFLLREEPIPMNQGYQYVDMFLGDYFIRKCMWSTPSTIKSTAASLKKFYKCM
                     LEHYMVKKEDYQELTETIKENMDEWLEDCSMYNDPDQDNPFYLFDV"
     gene            11974..13311
                     /gene="mgtE"
                     /locus_tag="PKH42_00625"
     CDS             11974..13311
                     /gene="mgtE"
                     /locus_tag="PKH42_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019279453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOA18959.1"
                     /translation="MEKLDILELIEQKKYIQLKKELSEMNEVDISEILDPLDLQTTLL
                     VFRMLPKDTAAEVFSYISPEQKADIINAVTDKELKYILDELFFDDMIDLIEEMPANVV
                     NKILINSTQEERTLINQFLKYPAHSAGSIMTIEYVELKKNMTVKEALEHIKKTGINKE
                     TIYTCYVTDKKRKLEGIVSLRNLVVSDPDQSINDIMQEEVIYVETHDDQEIIANIFKK
                     YGFMALPVVDKEHRLIGIITFDDIMDVMEDEATEDFQRMAAMVPSDEAYLDLSVIALA
                     KQRIPWLMILMLGATVTGGIITKFENILSTVVVLTAFIPMLMDTGGNSGNQSSTLIIR
                     GLALGNVNQKDIWKVLWKEFRISLLVGIILASVNFAKNMLFDKVGLNVSLTVSLTVAT
                     TVMLAKMVGGTLPLIAKKLKLDPAIMAGPLITTIVDALVLLVYFGVASALLIK"
     gene            complement(13351..14139)
                     /locus_tag="PKH42_00630"
     CDS             complement(13351..14139)
                     /locus_tag="PKH42_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA18960.1"
                     /translation="MKKIFKSILLTTSLTIGLSMFVAADTYAATYTIAKGDSLYKISK
                     AYNTTVSNIMVANDLKSYDLSIGQVINIPDVKYIVKPGDTLYKISQKYNIPLFNLRQA
                     NNIWTDYIYVGQVLEIPLPPAAVKEELKVAKQPNEGYKDYTAEELDLLARLIMAETES
                     QSYEAKVAVGSVVMNRVKSGLFAPTISGVINQNINGYYQFTPVENGWIKRAANEECIK
                     AAKEALSGVDTTNGALFYYDDSTTNQWILSKPVSVKYGRMIFAY"
     assembly_gap    14456..14555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14592..18044)
                     /locus_tag="PKH42_00635"
     CDS             complement(14592..18044)
                     /locus_tag="PKH42_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA18961.1"
                     /translation="MALVKLNINGKELTAEAGKTVLEAALENGIEIPHLCFDERLEVY
                     AGCGLCIVEIEGQPKIARACATPVSDGLVVRSDTQKVIEARNNALNLLVSQHIGDCKA
                     PCTLNCPANTDVQGYVGLVANKQYIESLKLIKEKLPLPACIGRVCPHPCEKACRRQYV
                     EEPVSIACIKTFAADYDLNTGNPYIPELKPATGKKVAVVGAGPAGLSAAYYLLADGHE
                     VDIFEAMPKPGGMLRYGIPEYRLPKNVVDAEVAIIEKMGANFNYGVKVGEDISLEYLQ
                     NKYDAVFLGIGAWKSSPMRCEGENTPGVLGGIDFLIKVAENKPVKIGEKVIVVGGGNT
                     AMDVARTSIRLGAKEVRVVYRRSEEQMPAEEIEIKEAKEEGVIFSFLSAPSLVLSDGE
                     KVTGLKCEKMVLGEKDASGRQSPLPTGEFVEFEADTIIAAIGQEVDCGKINVGQGKKK
                     NIVINEGTFETNLKGVFAGGDAATGPKIAIEAVAQGGRAAAVISSYLAGEMIPYKNQP
                     LVYTEVTKEDYKDEERTPRVPHRTVEPEIRKHNFQPILPTMTEKEAIREGQRCLECGC
                     KDYFECQLVDYIKKFEVDTKKYHAENEKKFKETDHPFISQNVDKCVLCGLCVRACDEV
                     VGASALGFVERGNATFIAPAFEQELKVTSCISCGQCIDVCPVGAWLDRRGNMKEIPLD
                     LTQTKSVCGYCSVGCDVVYEHKDNMVYLASSPKENEIPVCGKGKFGIEHINDENRILQ
                     SKVKVKGKYEPIELPVALFETAKRLRSIQNMYGDDQVAFVVSPKLTNEEFKYIKAVAD
                     ELNTKYKGSFTLDSESGIEYVLGKNESTIRAEELDNADLIISAGQLYEHHPAAGMKLR
                     RLSNTKKIISASTIKTKLNNFAVTADVTDYEVFFTRVLKALVDNGVIKKEAISRYENG
                     EELIKALDKTEVLPEAAKVAEAFKKARKPIIVADENTVPKAALKVLADITVLAGNNNR
                     PHRGLITLKAKANSQGAWNIGFRTPGEEIRKALLNNELKGLVVIGEDILGNVPELKKA
                     ADNLKFFAAFDIMENETTSNAEYVLPLCSIAESNGTIVSADGTVRNVYQAIKPLTGTS
                     NLEMFAKLAGLLNAEYKEDAKDEAKVKLYVPTLKGKEKEYEVYDTVEKAFLAKLKENC
                     IKAV"
     gene            complement(18060..19862)
                     /gene="nuoF"
                     /locus_tag="PKH42_00640"
     CDS             complement(18060..19862)
                     /gene="nuoF"
                     /locus_tag="PKH42_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOA18962.1"
                     /translation="MAIFRAHVLICGGSGCTSNKSQVVRKRFEEKIKEVQLDKEVQVV
                     GTGCFGLCEEGPVVIVYPEGSFYSRMTVDKVDEIVEEHLLKGRIVEKYLYKEKVKVED
                     AKEHKSLMEVQFYSKQKRVALRNCGLINPEDINEYIARDGYMALGKVLTEMSPQDVIQ
                     LMKESNLRGRGGAGFPVGLKWGFAAPNKADQKYIICNADEGDPGAFMDRSILEGDPHA
                     VLEAMAIAGYAIGATKGFIYVRAEYPIAVRRLHIAIDQARELGLLGKNIFDSGFDFDI
                     ETRLGAGAFVCGEETALMQSIQGNRGMAKVKPPFPANKGLWGKPTVINNVETLANIPQ
                     IILKGPEWFKSFGTEKSPGTKVFALGGKITNTGLVEVPMGITLREVIYDIGGGCPGGK
                     AFKAVQTGGPSGGCLTAGHLDTPVDYENLAAVGSIMGSGGMIVMDEDNCMVDIARFFL
                     DFIVDESCGKCTPCRVGTKRMLTILERITQGKGTLEDLEELESLANSIKNSSLCGLGQ
                     TAPNPVLATLRYFRDEYIAHVVDKKCPAKNCKALLTFEIDKDKCTGCTLCARNCPVSC
                     ISGTVKNPHEINQELCIKCGNCYSVCRFGAVQKN"
     gene            complement(19887..20252)
                     /locus_tag="PKH42_00645"
     CDS             complement(19887..20252)
                     /locus_tag="PKH42_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOA18963.1"
                     /translation="MKSLEELRKIREEAIKNIEMRNPDKDIRVVVGMATCGISAGARP
                     VLSTFVDEVAKRNLKNVQVVQTGCIGMCTYEPIVEIYSPDKEKVTYIHVDSEKAKRIV
                     AEHLVNGRVVREYTIGSEI"
     gene            complement(20264..20746)
                     /locus_tag="PKH42_00650"
     CDS             complement(20264..20746)
                     /locus_tag="PKH42_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA18964.1"
                     /translation="MQNEFLQSKKSEFAELKEYIDSVKNSQGVTMQILQKAQSLFGYL
                     PLEVQQFISDETNIPLADIYGVVTFYTQFSTEEKGKHKIGLCLGTACYVKGSQGILDK
                     ISDELNVKVGKTTDDKLFTLEATRCLGCCGLAPVMMVDDDVYGKLDTTKIPGILKKYK
                     "
     gene            complement(20927..21391)
                     /locus_tag="PKH42_00655"
     CDS             complement(20927..21391)
                     /locus_tag="PKH42_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18965.1"
                     /translation="MKKRFIFTMIVLLLFSYNSINGYEYHKEIETIEYLLNERITIMN
                     EFLYGYKDMQSLEEKLSKIESDNLLQNDLDILYKVKNSPTDYELAMKVKVNEIYDIEL
                     TDDGYLVNADLNWLMRGYDGEFNLIRNYQIKCIEDGGQSYLAKLTVLNNTAE"
     gene            complement(21509..22510)
                     /locus_tag="PKH42_00660"
     CDS             complement(21509..22510)
                     /locus_tag="PKH42_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA18966.1"
                     /translation="MITIKDVARLAEVSISTVSRVINDSKPVSPEVRKRVLKVIEETG
                     YKPNDVARSLVTRRSYLIGVIVNNLANSYVADTVRGIEEIGKMYGYDILLCSSYGSKE
                     TQEKYLQLLDRKQAEGLFLVGSIFDEEIIKLAKSFNKPCVFFTRDIRENMNYITIDCK
                     SAIYEVTKYLFEEGHRKIAYFSDYENRTTVEDEKVEGYLKAIEDNEMEYSKIYVVGGS
                     KHNEAYDLGKSIIDEAKDFTACVCSSDEIAIGILDSFLDNGIKVPDDVSIVGFGNIRE
                     GKFVRPELTTIGEPYYDVGAVGMRMLIKMIKGDKIQQGRMELPFTIEKRKSVKNLNK"
     gene            complement(22719..22892)
                     /gene="ssrS"
                     /locus_tag="PKH42_00665"
     ncRNA           complement(22719..22892)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKH42_00665"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23002..>24422)
                     /locus_tag="PKH42_00670"
     CDS             complement(23002..>24422)
                     /locus_tag="PKH42_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA18967.1"
                     /translation="ETYQIISIIGVIIFIYFIFFKNRRSAQKEISSLNEIEYIGSSTM
                     KFDDVAGNYEAKQSLLELVDFIQNPEKYSKFNARMPKGVLLYGEPGTGKTLMAKALAG
                     EAGVPFYAVSGSDFVQVYAGLGAGRIRNLFKKAKKSGKSVIFIDEIDAIGKKRMSGSI
                     KGSDEGDRTLNALLTEMSGFADDSGTVVIAATNRIDTLDSALLRPGRFDRQIEIMLPD
                     KNARKDIIKLYLDKKNAEDINIDELADLTVYFSGAMIENLINEAALLAIRQNSDKIKE
                     EHIKKAYLQILAGVEKEDYDNGFQKTITSYHEAGHAFVSRHLLPENKIIRVSVIPTSK
                     GAGGYTLSTPDSEEVMTKGKMINHLSVLMAGRASEEVFFGNENITAGAQNDIEKVTSL
                     AVDYVSKYGMNDATFINYQLLSDKLRIPLTSVEVSVKKLTSEIYEEVIRLIECNKEKV
                     EKIARLLMEKEVIYQDELDNLI"
     assembly_gap    24423..24522
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24616..25095)
                     /gene="ispF"
                     /locus_tag="PKH42_00675"
     CDS             complement(24616..25095)
                     /gene="ispF"
                     /locus_tag="PKH42_00675"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOA18968.1"
                     /translation="MKIGIGYDVHALAENRKLIIGGVKIPYEKGLDGHSDADVLIHAI
                     MDSLLGAMGKGDIGRLFPDDDAKYKDADSRVLLRKVYEIMRENKFTVGNIDAVIIAQN
                     PKLSPFIDEMRRNIASDLKTDISSINIKATTTETLGFIGRGEGIGAECICLLETKEK"
     gene            complement(25106..25798)
                     /gene="ispD"
                     /locus_tag="PKH42_00680"
     CDS             complement(25106..25798)
                     /gene="ispD"
                     /locus_tag="PKH42_00680"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOA18969.1"
                     /translation="MQNKKHISAIIVAAGKGKRMDSDIPKQYMDIAGKTILDTTLYKF
                     EKSNDVDDIILVVNKEDIEFVKDEIAPFYRKIAYVVPGGKTRTESVYQGIKKVSKNCN
                     IVLIHDGVRPFVSYNLISTCVENAIIHKACVPVIDVMDTIKEVSEDGMVVKTFDRKKL
                     RAVQTPQAFDYDIIRECYEKAMTEEKEFTDDASIVEYYGYKVKTIEGLYKNIKITTPL
                     DLRMAEIIVNFF"
     gene            complement(25827..26639)
                     /locus_tag="PKH42_00685"
     CDS             complement(25827..26639)
                     /locus_tag="PKH42_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOA18970.1"
                     /translation="MKKVDLHIHTNASDGTWDVTELKNELKKNKIDIFSVTDHDSVEN
                     IENMQNILKNKDNLIFIPGTELSAEYKGKEYHLNLYNYDIKNKELLELMKWTNQNKLN
                     SNVDYINYAANKYKNISVEDFERYANDRKRGGWKSANYMIDKGIHKDIPEHLKDVYES
                     GLKAELKSPEEVINIAKKSGGKIFLAHPSYHYRDSVMPIEELKHWGELGIDGIECYSP
                     YNRDNTRYYIDFCKKNNLMISGGSDCHGSFIPSRKLGVPDVSLDDLNIKKLL"
     gene            complement(26685..27680)
                     /locus_tag="PKH42_00690"
     CDS             complement(26685..27680)
                     /locus_tag="PKH42_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOA18971.1"
                     /translation="MKKILMIATGGTIASKITEHGLAPAISSDELLSYVPEIKKYCYV
                     DTIQLLNIDSTNIQPEHWVMMTETIEENYDKYDGFIVSHGTDTMAYTAAALSYLIQNP
                     CKPIVLTGSQKPINVDITDAKKNLLDSFRFVTEEGISGVYVVFDGKAIIGTRARKVKS
                     KSYSAFESINFPVAAIIDDRRITKYISNYNLEKKVKFYKEIYTNIFLLKLAPGMEPDV
                     LDFIGNKYEGIVIESYGAGGLPFLDKRNFLNKLEELIKKGKIIVVATQVMFEGSDMGL
                     YEVGVKALQKYNVLQAYDMTIEAVITKLMWIMAQTKDFEEVKTMFYTRIHEDSLY"
     gene            complement(27689..28570)
                     /locus_tag="PKH42_00695"
     CDS             complement(27689..28570)
                     /locus_tag="PKH42_00695"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA18972.1"
                     /translation="MFNNILKYTIQENDETVKSILTEKLKFSGRLSRKLERNGKILLN
                     GKAVKLNKSIFAGDELSIEFDEEEDRYDAVDMPLDIIYEDDDLLVVNKEPHMVVHPTR
                     SHQNDTLANGVAYYFKKNNIKRKVRFINRLDMNTSGIVLIAKNPYIHNQMVLEMKSSM
                     VEKYYYAIVEGLLEEDEGRIDVPITRLNPEDIIRTVHTSGKECITEYKAEKRYNNMTL
                     VKLKLITGRTHQIRVHLKHIGHPIVGDTLYGNESLLIDRQALHCYEMKFKHPITNKEL
                     IITCPMPEDMKRVIEGR"
     gene            28699..29502
                     /locus_tag="PKH42_00700"
     CDS             28699..29502
                     /locus_tag="PKH42_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOA18973.1"
                     /translation="MKEKVINIAANDDVYTADIKKRLIKVLTENGYDYYEGFKEDAQL
                     NITVGGDGAFLRSVRESNFSQIPFIGVNTGTLGFYPEITPENLENFIINYRAGNYKIN
                     PVNLIECEIVSANLSNKIYAVNDIILKRIDMKTLHLNVFIASNHLEKISGDGLIISSP
                     LGSSAYNKSAGGSLVYPSLKTLQITPLSPILSNAYRCLECSIIVPPEFEIAIYPERRE
                     DYCLAILADGEIYEYDDMEHVLFRTSKKVINRLSTGTFNYWNVIKEKFL"
     gene            complement(29522..30787)
                     /locus_tag="PKH42_00705"
     CDS             complement(29522..30787)
                     /locus_tag="PKH42_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA18974.1"
                     /translation="MFRKLHLKLTAYMGIILILFMFFVSAGIYQFTKMVFEDGNKELM
                     RAEALRIYNYKKTAYTQYFFNGTLFKRYIQINPSENRRLDACYVVYDQYMIPVLSEEG
                     EIKIADHIKPLAQKAFEEKKDSYETQKIGRFNYRIYTRYFSDYTGQSVVQVYQNTVNE
                     DILWSFLRTVLIMIGITGTVILLVLSYMLTGKALAPIKETWKRQREFVADASHELRTP
                     LTVIQTNLDVVLSDEDGTVDENEIWLDNAYSETRVMARLIDQLLILAKADNNEEELDI
                     SEFSFSEVIENVCQSMEVIAAKKNIELITNIEDNIIIKADYDKMRRLAVILIDNAVKY
                     TEKGKVTVSLKTDKNKKVLIVEDTGIGINEKDLERIFDRFYRADKSRHREGGVGLGLS
                     IAKWIADKHKYSLTVESTINVGSKFILRM"
     gene            complement(30787..31455)
                     /locus_tag="PKH42_00710"
     CDS             complement(30787..31455)
                     /locus_tag="PKH42_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA18975.1"
                     /translation="MRILIVEDEVKITQALSYLFNKQKIDVDIANDGEEGLILASKDI
                     YDVIVLDIMLPGVDGIQILKTIRKDGIKTPVIMLTAKDTVDDRVFGLESGADDYLVKP
                     FATKELIARVKALARRKSTEFVGEIYEFEDIKYDVKNYLLYVGDNEYKIPLKEAMLME
                     MFIKKPRQVFTREQIIDRIWGLEADILESNIEIYVHHLRKRLENTNVKIETVRGVGYM
                     LKEK"
     gene            complement(31663..32490)
                     /locus_tag="PKH42_00715"
     CDS             complement(31663..32490)
                     /locus_tag="PKH42_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017695304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOA18976.1"
                     /translation="MRIQHNLNAMNSLRQLGTNNTNTGKNLEKLSSGFRINRAGDDAA
                     GLAISEKMRAQINGLEMATKNAEDGISLIQTAEGGLNETHAILQRMRELAVQAANGTN
                     QAEDLQSIQDEIDALADEIDRIGDSTKFNGIDLLNGGSMASDGIDFQIGANNADYESV
                     NLTIASMKASDLGVDTIDVTATGDPLGAIDAIDAAIADVSAARAGLGAMQNRLEHTIN
                     NLGVTTENLTAAESRIRDVDMAKEMMAYTKNNILTQAAQAMLAQANMQPQGVLQLLQ"
     gene            complement(32644..33936)
                     /locus_tag="PKH42_00720"
     CDS             complement(32644..33936)
                     /locus_tag="PKH42_00720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA18977.1"
                     /translation="MYITQCLIAKNEEANIEHCLSHLKTVVDEQIVIDTGSTDRTVEL
                     AEIMGAKVFHFDWINDFSAARNFALDKAKGDWIIFLDCDEYFSEDSVPLIRKCIKKFG
                     RQKEIEGLTTNFINIKSNGEIISAVKNVSARIFKRKSYLRYKNKIHEVLVNVNKTDDN
                     IVRADISDLLKIYHTGYDKTIVNEKNKNERNFQLLERELKNNPKDIKINYYMSRQMNI
                     EGKYEKAIEYGLKAISYIDDFYEFDYYCTIYSGIMINMLSLLKSYDEIKVIFDDAIKK
                     YPGYPDYYWLLGIAAVRGNKYEEAVNLLEKCIKLCEKYNCNAESLALGKIDDVYIELL
                     NAYNHQNNKHKIVKTAVSILKVNKYNIQVLIILLNTFLTQEKEENIVAFLNKIYDYNL
                     FKDKIYLFKASKIVDNIILVEFFRKLFNDEELKKINNI"
     gene            complement(33968..35083)
                     /locus_tag="PKH42_00725"
     CDS             complement(33968..35083)
                     /locus_tag="PKH42_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOA18978.1"
                     /translation="MKKYLLYGHGGAYNHGAEAIVKSTVDVIKKRHPDSKVILSTHFK
                     EQDIEFNMPIDEYCERDMKYVELDKNSSEKGIYDRFIYKSTLEQITEDTVCLSVGGDN
                     YCYDNWRKWKAIHERAIEEGARSILWSCSIEPSMMDNEMLDVLKSHHLITARESITYN
                     ALKDRGLDNIRLCSDVAFLLEPKEYSLPENFFVGNTVGINISPLLIRREKTEGIVIKN
                     VIKLINLVINKTDMNIALIPHVLMPMDNDLEILEKIFNRFKDTGRICFVNKNISAAEY
                     KYVISNCRLGVFARTHASIAAYSTFVPSMVIGYSVKSKGIAKDLGLEDFVLPIEEFHN
                     DCCMVSMFEELMNNEQNIKKILADKIPLYRKRAMPNY"
     gene            complement(35080..36387)
                     /locus_tag="PKH42_00730"
     CDS             complement(35080..36387)
                     /locus_tag="PKH42_00730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA18979.1"
                     /translation="MSKIIIYTPAYNAEKTLRRAVDSILNQTHKEFIYYLLDNASTDG
                     TYEIIKEYAKNDKRIIPLHNEINWVGNGILDIIKNHENDCYMCELDADDEYTPNFLEK
                     MLFFIKNENLDIAACGSDFVDMQTNKHLGMRLLEKNLILDEEEFDRYFPKYHQFMRTI
                     WGKVYSISVLRRPEYKRYKQPGYGNDTLFAMNAFYNANRVGILSELLHKYYVSPKSVS
                     YQFDKNRTKSDRVLFDAACDFLISKCGAVSDENLNFLWIVYFNAIKDTLNVLLNAQIS
                     VSDKFEYLLDIFQSRHTQELIKWPGAKAEKNRLFSQAASRMLSSSPYLVGLEVGFLCY
                     FKDIVFSILQNDEENALSQIIELIAEGTEIPDEYVESLMLLALNLSAKLESHDYFIYF
                     KKLQISLLIELSKTNEAIKEFADWDEILSDDIDFKNLKERLGI"
     gene            complement(36424..37458)
                     /locus_tag="PKH42_00735"
     CDS             complement(36424..37458)
                     /locus_tag="PKH42_00735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA18980.1"
                     /translation="MVYIFTTAYNAEKTLHKAVNSILNQTYTDFCYYLFDNGSLDDTR
                     KIIKEYANKDNRIIPLFGESNCRRVIPKIIKDIISGDNMLKNSYFATLDADDEYSPDF
                     LEKMIDFVEDNNLEVATCGTEWIDEKTGQTIKKKVLNKNLILEGKEFADKFPLYRNFI
                     VTLWGGIYSLELLKKCDFEWYHNAMNFSDTAFCMETFRRAKRAGVLSESLHKYYISPR
                     TVSYKYNPDWFYACKYLHKISREYLLDYGEISKQNENYLCVIFLILIKYILPKIQQAD
                     VKLSEKLKSLHNIFTDDMTKYVLEHWDEVGIYSNKLDFLREIESWLYTQDGWGLLRKT
                     VEEIITAMNI"
     gene            complement(37468..38280)
                     /locus_tag="PKH42_00740"
     CDS             complement(37468..38280)
                     /locus_tag="PKH42_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA18981.1"
                     /translation="MNKRVELYHSGLTITQKCTLKCKLCAGYSPYYNPQPHYSINRLS
                     EILRRYFSIVDYVEKFTITGGEPFLHEDLDKILIETIKYRKQFGKIEIITNGTLFPNE
                     KLKAVLEKYAVEDGCITLLVDHYGELSKKIHELGLYFIEKGINHKIRIYYGENAHCGG
                     WVDFSDFSKKLFSVSQMMEKFKKCAIPSKLNYCLAIKDGEMHPCTFSHRIMELNLIER
                     NKDEYIDLFDDNTIEQQKEKIMKLFDIKYLSACAYCNGMCDDSVRFVPAEQL"
     gene            complement(38293..>38521)
                     /locus_tag="PKH42_00745"
     CDS             complement(38293..>38521)
                     /locus_tag="PKH42_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18982.1"
                     /translation="KGVSLLLINSNKGKHYLEEISKYISKEQKTLKDATLKNPCILNS
                     VTLNNKRELLFKALRKETDVVDIIQKIIDYD"
     assembly_gap    38522..38621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38622..39130)
                     /locus_tag="PKH42_00750"
     CDS             complement(<38622..39130)
                     /locus_tag="PKH42_00750"
                     /inference="COORDINATES: protein motif:HMM:NF016319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HOA18983.1"
                     /translation="MNNINFSRALEKSCCTGCAACANVCPNNCIFMEYDDEGFLYPEI
                     DIKKCTECGLCEKSCPAINNPVNDNFIEPEVYAAWSLDDEVRASSSSGGIFTELAHKV
                     LDNGGFVSGARYKTNNLVEHCLINSREELHELRQSKYVQSKIGLIFRDIKKLLEEDGT
                     VMFTGTPCQS"
     gene            complement(39153..40130)
                     /locus_tag="PKH42_00755"
     CDS             complement(39153..40130)
                     /locus_tag="PKH42_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA18984.1"
                     /translation="MGKKYGTLEGMVDMKISVIMLTYNREELLTRAIDSILNQTFKDF
                     EFIIVDNGSTDRSGAIADEYALKDSRIKIIHREQGNIGSGRNAGLDAATGEYVAFIDD
                     DDYAESDFLEFLYNLAKKHNADIAVCGSTKEENGQQLPNGIYQYDEKYIMDAEQATVN
                     FLWRKLYNAAMPTKLVRREMFDSIRFLNEGKYDDITTTYRYFANAKIVAAHGLPRYCF
                     YRHSGNNSSAATKHHLLNPVQLNEYLAAFRERTEYISKILPQLAGLALYSEWSYMISM
                     VEKIHRYGLNNCADLLVLMCDNLRAHWDDFYYGKYILEFEKVWMERYVK"
     gene            complement(40094..40870)
                     /gene="rfbF"
                     /locus_tag="PKH42_00760"
     CDS             complement(40094..40870)
                     /gene="rfbF"
                     /locus_tag="PKH42_00760"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOA18985.1"
                     /translation="MKIVILAGGFGTRISEESHLKPKPMIEIGDAPILWHIMKYYSCF
                     GFNDFIICCGYKGYVIKEYFADYYLHRSDITFDFSNGNEMIIHNNVAEPWRVTVVDTG
                     LYTQTGGRIKRIKKYIGDETFMMTYGDGVSNIDIHKLVKQHKSTNNIVTLSAIQPGGR
                     FGVLDIDENDRKVIGFREKATEDAGWINAGFMVLEPAVFNYIKEDKTIFEREPLESLS
                     AEEKLGVYKHYGFWQCMDTQRDKYHLESMWGKNMAPWKVW"
     gene            complement(40889..42784)
                     /gene="fliD"
                     /locus_tag="PKH42_00765"
     CDS             complement(40889..42784)
                     /gene="fliD"
                     /locus_tag="PKH42_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOA18986.1"
                     /translation="MSILSTNLYGSSVNKGMGGLMSGLDTDDLVNQMTAATRNKINKQ
                     YQAKQKLLYKQEAYREISSKLLAFNNKYFSYSSGSKTNILSPKFFESYTYKSSSNYVN
                     VTGSADVISNFKINSITSVAKAAIYTTNSVSSQSFSSDEIIGNISEDVLLSSSITFDF
                     NGVKKAIALSEMVDGKPDGSPYTYDAEGLADFLNTKLAEVYGAGRVNAELDASQTSIM
                     FSASGSTDVFSVSNISNDLSILTGIKSGDSNRLKVSKTIGDIYGEVTDCSIIINGKSI
                     DGISKDMSISEVLKKINKESDLDITYSNTTDKFVIVSKDTGKASKIEITGALADKLFG
                     GSIGSDIEIRGEDTVMNVTIHGQDIDITRSTANFSIDGINVELGKNAILSPEENPITF
                     DVKNNTDEVIERVKQFIDDYNEIIDLLGTKTKEKPRRDYPPLTPEQQDEMKEKEIENW
                     LKEAKKGVLFGDKNITSLLNKMRASMSGMTSVSDLALSNIGISAASMDTSGKLVFNEE
                     KFREKLIEAPDQIASLFTGIADETDEYAKSGIANQILDILRENIGAMGTSGKLIEEAG
                     LDTGRSSDQNNISLKIKDYDEKMKELKKSLERERQRYWNQFSALEQSLNKLNAQSSWL
                     MDMMGGY"
     assembly_gap    43102..43201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<43202..44808)
                     /locus_tag="PKH42_00770"
     CDS             complement(<43202..44808)
                     /locus_tag="PKH42_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOA18987.1"
                     /translation="MKTNKSISKKITTTILIVCFSALLITGIIIVKTVSTRFKDNKRQ
                     ILNETALSISNKAEVFFEKYITVVEQMAHDKNLQEFMINAEGYQTLPVTRGFDIATAT
                     TDDIQKADSEIILTAYIAEDDYYLASPTIYTQSEYDVTSKEYYNAVTENKVCITDPYI
                     DAITGGIVITISAPVNVDGEIVGLTAVDIAIDKLINMVDEYELGDTGYFTLLSKNNVI
                     IAHKDKDNLLKKVDEINLSDNIVQSVINKDRSVVEFTYNYDRFIGNSVEIGDTGWQIM
                     SSLPREEFMLNTNQLVLIIIAVFVILLVVLIGVLYVLINKLTKPITDITSITNKLAEG
                     ELDVNIDINSDDEIGVLANSIRKLTERLKLYIIYIDESVGVIDKMAEGHLNMELRNDY
                     AGEFAKLKKSLLNMSNTLKDAIGKIQQSSIAINMNAEQVSTGAQTLAQGTTEQASAIE
                     ELSAEINEIYTTIVNNAEYAENAGSKAMQASKEVEHGNMQMREMLSAMDEISNSSSEI
                     GKIIKVIDDIAFQTNILALNAAVEAARA"
     gene            complement(44846..>45130)
                     /locus_tag="PKH42_00775"
     CDS             complement(44846..>45130)
                     /locus_tag="PKH42_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18988.1"
                     /translation="SLIIDYNRVNSLLDERQQYIDKINVINEKISEVKSKENYVETNE
                     IKKLNKDISRVFTEIYEIDKVIRKNINTELKSVKEKLNYSETNIVVNIKI"
     assembly_gap    45131..45230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45231..45450)
                     /locus_tag="PKH42_00780"
     CDS             complement(<45231..45450)
                     /locus_tag="PKH42_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HOA18989.1"
                     /translation="MLNSLEQYKKTSVNTMTKGELLILLFDEAIKKLNQSKLLMEHED
                     YANANIFLTKVRHIFYYLTATLDGKYQIS"
     gene            complement(45517..46092)
                     /locus_tag="PKH42_00785"
     CDS             complement(45517..46092)
                     /locus_tag="PKH42_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HOA18990.1"
                     /translation="MLINKIIVIGASTGGTEAIIKILKELPEDFPPILIAQHMPIGFT
                     KMYADRLNKICKMEVREALNGDRIKQGIVLIAPGDKHMVLKKDDKGYYVSCKKGDKIN
                     GYCPSIDLLFNSAAKIAGNKSVGIILTGMGKDGAEGLLNMKKAGAYTIGQDESTSAVY
                     GMPMAAFNIGAVTVQAKLEDIFEILTKHLTI"
     gene            complement(46094..>46438)
                     /locus_tag="PKH42_00790"
     CDS             complement(46094..>46438)
                     /locus_tag="PKH42_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOA18991.1"
                     /translation="IYSKDSICKLPDSFIKRYFIKLDDNKYQVVPEIKNYVRFKASNL
                     MAPILEKNRYHVIFMRNVMIYFDLETKNKIINKLYNATRSGGYLMIGHAEIIQREQSK
                     YLYIKPAIYKKE"
     assembly_gap    46439..46538
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<46539..46713)
                     /locus_tag="PKH42_00795"
     CDS             complement(<46539..46713)
                     /locus_tag="PKH42_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18992.1"
                     /translation="MVRITDNEFLEYVKYINKNYGIDLSKKKILIEGRLSNLIEKKGM
                     NSFYQYLRSVKNND"
     gene            complement(46697..48733)
                     /locus_tag="PKH42_00800"
     CDS             complement(46697..48733)
                     /locus_tag="PKH42_00800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOA18993.1"
                     /translation="MDNNLDSVLDMYLFETNSLLELLDNILLKAESNLSFDKECMDEI
                     FRAMHTIKGSSAMMQFDSIMTISHKIEDLFYYIRENGIDQKYNEELVNLVFKITDFIK
                     GEIVKVEEGLELEKNLDPLENEIKLFLNSIKKETKEEIYQIENLKSNIAITEENNQTK
                     GCTYAIRIIFDDDVSMENLRAFIIVNQLRETGIEFEYKPENIENIPETSQEIIENGFF
                     VYVNKEYLDTATEVISKALNVKNYTVFEYNEEVQNNKEDETIISQTRCSTDLNNSISS
                     TNSKQCRQSLITVNLSKLDKLVDIVGELVIAESMVESDADIKRLNSESFKKSTRQVRK
                     LTDELQDIVMSLRMVPISGTFNKMHRVVRDMGKELNKDVELVLNGEDTEIDKTIADCI
                     SDPIMHLVRNAMDHGIEPEEKRISKGKNKKGKITLSAFNTGGEIHITVEDDGRGFDSE
                     KILEKAEAKGLLTKPASEYTQKEAFQFLMLPGFSTNDDITEYSGRGVGMDVVKSNVEK
                     VGGTIVLESNEGLGSKITFKIPLTLTIVSGMQVVVGNSEFIIPIKSIHQSFKTKKSEI
                     IHNPEQGEMILIREKCYRIIRIHEYFNINTEITNIEDGILIFVESGDNSACLFVDKII
                     GEQQVVVKPLPIILNKYNLKESGIYGCSILGDGSINLILDVGNLMESNRYGENN"
     gene            48959..50374
                     /locus_tag="PKH42_00805"
     CDS             48959..50374
                     /locus_tag="PKH42_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA18994.1"
                     /translation="MNNISVKTNNHISENPINKPKIKNEVPFDIADPTKVTKTPEKGY
                     ENQIRQELFNYNPDSVFDKFIKSLQNSPVLYEEAKKLLLSKQFINNNIKNDPVLSVFF
                     DSFLGNIKMNEAEILDFLKFQQGTYTKFQGEFFDLLRTLLGNNPDNKGLQTVLKNFLR
                     SYDCFVSVKEIYGSINTTFKNIEKNIPEMLKASFNELTEKFIYDNTNAMDSNLELLKN
                     EILPFIGRYISKMNDFGIIRDYVSVLVHNLVRLEYGSKTNFSDELENLFEFLKYNFQL
                     DGKQMELLKTTLINTYETKSNIKNDSIESFFKLLDKGISESENAVTKGLMGDMTESIL
                     FSQNVNIPLLHMFLPLNYKGMFMFSEIWIGKDEDSPDNKRNLEKQKSYKVFITFDIQN
                     LGYFETTLKLRDSVLSLDIYIPGSLSEQTEKIKSDLNFLLSKNNVTIESIRVQECIKA
                     RRFSEVFTNLTERKNGVDVTI"
     gene            50361..50642
                     /locus_tag="PKH42_00810"
     CDS             50361..50642
                     /locus_tag="PKH42_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOA18995.1"
                     /translation="MSQYNNKSNHAVALKYNAAKNNAPVVIASGSGYIADKVVEIAEK
                     NGVPVYRDDSLSAILSQLKIGSEIPEELFSSIVDIYVYFLNFKMPESEA"
     gene            50665..51306
                     /locus_tag="PKH42_00815"
     CDS             50665..51306
                     /locus_tag="PKH42_00815"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOA18996.1"
                     /translation="MIDTDKIVKIYDEDSKLIGKGQLLSLNEKIIKVKGTGLPILSRK
                     TNVIIEIYSEFVGISRYCCQIDLASDNQLNAHIVKKKPDIERRNSLKVRTDLSYYIES
                     LYRNDKDITKDFPNMKINLINLSIGGMLISSNYELYINDIIAFYFRCEKTAVLLKAKV
                     MRIDKIYDDNQKYLSFYNYGCIFEEMPPHIEKIITRYLYIRQLEMHKDKWEKL"
BASE COUNT        14608 a         9970 c         7291 g        18791 t
ORIGIN      
        1 ttaaatcttc agcaatttct tctatttttc ttagtctgtg atttactcct gattttccaa
       61 ggggagggtc taacatttga cccagttcct ttaaacttgc attgctgtgt ttaagtctta
      121 agtatgcaag ttcccttaaa ttatccggaa gttggtctat aaccttgtat ttttttagta
      181 ttttaatatt gtttatctgc ctcattgatg tgtctacaat tttatccaga tttgcagttt
      241 cacaattaat tactctgttt atttgatttc ttgtttcctt aacggctctt atgttttcaa
      301 aatccaagac agcgttattt gcgcccatga gagctaacaa atcggaaatt tgctcgcttt
      361 ccttaatata tgtcacatag ttgttctttc tgtaaatatt cttaccgttt attccatata
      421 ttttcaaaag cttttgcaaa gtcttgctct gtacttcctt gttgcttaca aattcagcat
      481 gatatgattt ttcaggattg cttatcgaac cgctgcccat aaatgaacct tttataaagg
      541 ctctcctgca taagtcgctt gccagtaatt cttcaggtac ttcataatta aaattcatta
      601 cattaaaatc attatcttta ataatttgac tgtctgaaag aaattgcttg gttaattttt
      661 catctatttt aattatataa ttattttgct ttttcaatcg attgctttta gaaacactga
      721 catctgcatt tatattatgg gctattttta aaaccttgaa tatccgtctt gcaactgctg
      781 cattttcagt caaaaattca aattctacct tatttagacc cttaattgtg atatatccca
      841 cagttctgac aaatgctgcc gcttcagcat tagcacataa tacatcactt atttctgttc
      901 tggacagttc atctttaact ctcgatgaaa atgtcatggc atcacctact tatatgaatg
      961 aaatttaata tcatctacag cttttttaat gattttcctt tcatcgtcat atcgagctat
     1021 tgtcaatgat ctttcaaagg gcaaaaattt tgcatctata aatccttcat ttttttgagc
     1081 agtgcagttc atatttcttg ccatcattaa ataccaatgg acttcttttc ttatgtggat
     1141 tttatcaagg tagcaggtgc ggctgaattc gtaacttatt ttacctaaat acttaatagg
     1201 agttactttt gctttcgttt cttcatatac ttcccttaag gcagtctctt tcaaggactc
     1261 gttttcttca actctgcctt ttggaagaac ccaatcgccg ttatactttt ttaacatcaa
     1321 tatgttgttt ttaaagaaaa ctattcctcc tgcacttatt tcatctacca tacttgcctc
     1381 accttttaat attgtagtct ttataatacc acaatattaa gaaaaatcaa acatatattt
     1441 acatatttta agtttannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttt
     1561 ctccgaaata ctgatagtaa tcaaccctca tatcgccatt gaaaagtttt cttttcctgt
     1621 ctgcttttct cccaaattct ttttcaaagt ttactgaaga agtgattaca tatactgacc
     1681 aagttttgtc cctgccaaaa acttgcccta attttttatg aattttagta agctgttctt
     1741 catcacctat tctttccgca tatggcggat tggttatcaa tactccatag gaacacatcg
     1801 gtttttcaat atgtgtaaca tcctttacaa aaaactcgat acagtcatcc actcccgctt
     1861 caattgcatt ttcttttgct attcttattg ccttttcact cacatctgaa gcatatattt
     1921 taatttctga atcaaaatca attctttttc ttgcttcaat cttttcattt ttccaatatt
     1981 catctttaac caatggccat tccatggaag caaagtttcg atttaaaccg ggagctatat
     2041 ttcttccgat taatgcagct tcaattgcaa ttgtacctga accgcataat gggtcataga
     2101 gcactctgtt tttattccag taactgagct ttaccaaagc tgcagccata gtttccttta
     2161 aaggtgccgc cattgatttt tctctgtatc ctcttttaaa caatccttcc cttgccccgg
     2221 ttgtatctat tgaaatcgtt gcaatgtctt tgtgaattga aactaatatg gtaaattcag
     2281 cccctgtttc cggcatccag tccgtatcat aatattcaca gagttttttg gaaattgcct
     2341 ttttaaccat cctctggcag tcaggggtgc tgtagagttt tgatttgacg gattttccgt
     2401 taacagtgaa ctttccgtct tttgtaatcc atttttccca aggaagctca taagttttgt
     2461 caaataattc ttcaaaactt aatgtttcaa attgtcccat aacgagcatt actctgtcgg
     2521 cacacctcaa attgatattt gttttgcata tatctttaat ttctgcatca aaatacatcc
     2581 acccgttatc agtaactata ttttcatatc ccagatctgt caattccctt tttacaactg
     2641 cttcaagacc aaaggctgat attgcagcca attttatttt ctccatacat ttcctcccaa
     2701 cggggcatcc cttcatggct acaccccgta tattaagggg acgggggtac cgtcatccta
     2761 atatttatca tccctgctta ttccgtattt atccatcttg taatacaagg tggctcttgg
     2821 tatttgtaat ttctttgctg tttctttctt attatagttc atttcttcca aggttttaat
     2881 gataattctt ttttctattt gttgaatgta ttcttccaat cccgaatagc tttttatttc
     2941 atttaaatta ataagcttct taatttcgtc aacttcgata tcctgcatgt atgaaggaag
     3001 atattctctt tcaattttat tctttcctct ctgagatgct attaccgcac atctttgtat
     3061 tacatttctc agttctctta cattaccttc ccatctgtat tcggaaaata tactcattat
     3121 ttcgtcgggg atttccacta tgctaatacc ctgctccatg cagaattcct gtagaaacct
     3181 gtttgctaaa agaattatat ctccttttct ctccctgaga ggaggtatgt ttacctgaaa
     3241 tatatcaagt ctgtaataga ggtccttcct gaatttatcc tcatttatca gttctcttat
     3301 atccttattc gtggcagaaa ttattctgac gtctattgtc tttggcttat ttcctccaat
     3361 tcgagttact acgccatctt ccaaaatcct tagaagcttg ggctgaagtt ctaagggcaa
     3421 gtctgctatt tcatccaaaa atatggttcc tccttcagct tcctcgaatt ttcctttttt
     3481 cccttcagat tttgctccag taaaggcgcc tgcttcatat ccgaaaattt cactctcaaa
     3541 caattctctc ggtatggcac tgcagtttat cggaataaat tttcctttcc ttccgctttc
     3601 ataatgaatt gcttttgcaa agagctcctt acccgtgccg ctttctcctt ttaacaatat
     3661 gtttagaggt gttttggata tgtttttgca caaatttatt atttcaataa aagctgcatt
     3721 attgctaatt attttagaga aatatgcttc tcccgaggaa gttacttttg aatattcctt
     3781 ttcaagcttc aataaatttg actgggtttt attcagtagt tcgcttaatc ttacgatttc
     3841 agtaatgtcc ctgtcccttg ccaatgcacc aatcatgttt ccattcctgt cataaagggg
     3901 agcagcactg gacacaacga agctgttttc cctggggctg ttgtatatat tataaaaaga
     3961 tgtcttgctg tccaatactc ttaacaatat tgcagtggga aaaaattcaa caagatcctt
     4021 gtctattatg tcgctttttt tcaaattaaa atattcctct gatgttgagt tgaaaaatat
     4081 cactttgccg taattatcaa ctattgttat ggcatcagga atgttgtcca gtatgtattc
     4141 aaatttttct ctgtcttgtt tgctgaattc tatttcatta ttcatgtaat cccccctgga
     4201 aacaataata ttttgggcaa gctctcatct gttgcatata ccaaagtggt atgcaataag
     4261 ttaaaattac ggatttatga ttgtctcgta gtaattttct ataaaaaggt tattgagatt
     4321 ccaatatttc ttgaattttt cgtattcaat ttccgctgat tctgtatttt tatttgactt
     4381 aactgctctt atgagaatgt ttttaggagt atgctccatt gatatgaatt ccattaactg
     4441 caccttatac cccttagttt ccaaaaacaa acccctcaat gaatcggtaa ttaaggagga
     4501 cattctctcc tttatcagcc cgtgcttaag catgggattt aaattttcat tttcaatttt
     4561 attgaagagt tcatgctggc aacaaggtac ggacaaaata atatcgcagt cccaggcaat
     4621 tgcctttaca agggctgcgt ctgttgccgt atcgcatgca tgaagagtta ctatcatatt
     4681 tactttgttt ttatattcaa aatcacttat atctccatat acaaaattaa gattttcata
     4741 gttaagtttt ttggctgttt cattgcaaaa tccaatcact tcttctttta aatcgagccc
     4801 tgttattttc acttttattc ttctaatata ataaaaataa taatacaggg caaatgtaag
     4861 atatgctttt ccacagccaa aatcaataat cgtaaagcca tccttaatac tttttcccgc
     4921 cagtgaatca tcaacaattt ccaagaattt atttatttgt ttgaatttat catacttctt
     4981 ggtataaacc ttgccatctt tattcataat tcccaggatt ataaggaaat cacatggttg
     5041 gttttcactt atcaaatatt gttttttctt gttatgctct tctttcttca gcttttttac
     5101 aggctcactt cgagtaatct ttatacttcc ttttttgctt accaatattt gataatctgc
     5161 atctgttgta aataaattta tattgcgata ttcattaacc atgttaagca agacatcaag
     5221 agcaacctca tagtctaagt tttcgtggaa agcttttgtc tcggtaaact tctccaactg
     5281 caggtactcc ctgtctttta ttgcaatagg ccttacagtt atttttttat attgggactt
     5341 aacccttggg gttgtaaaaa tacaatatat gaatttgttt tctgttagac tgttttttat
     5401 tatctcatca atattcaaaa taccacctgt aatttacaca cctttaaagc tgctttttaa
     5461 tgatactatt ctgttaaata ccaaattatc ttctgttgta tcctggtcaa taataaaata
     5521 cccgtgtctt aaaaattgga atctgtcacc tactttgaat tccttcatag aaggctcaac
     5581 cttgcagttc tttaacacaa cctttgattc cgggtttaac ttttccaaga aattggtttt
     5641 gtcgtattcc tcatcttcca tcatgtagtc ataaagtctt acttccgcat cgatgcaatg
     5701 ttttgctgaa acccagtgta gagtcccctt tacttttctg ccggtaaagc ctgatccgct
     5761 ctttgtttct ggatcatatg tgcaatgaac ttcagtaaca tttccgtctt catcctttac
     5821 aaaatcaaca cattttacaa aatatgcatt tctaagcctc acttcattgc cggggaagag
     5881 cctgaaatat ttcttcggag gattttccat gaaatcttct ctttcaatat atacttccct
     5941 tgtaaaaggt atcattctac ttcccatgtc agggttatcg gggttgtttt ccgtttcaac
     6001 atattcaacc ttatcttcgg gataatttgt tattataagc tttattgggt ctaagacagc
     6061 cattgttctc ggaactttag gtcccaaatc atctcttatg gcaaattcaa gcatagcaat
     6121 atcaacaacg ctctgagctt tcgagactcc gattgcttca cagaaattat gtattgcttc
     6181 gggagtataa cctcttcttc tgaatcctgc aatagtaggc atacgagggt catcccagcc
     6241 gtccacatac ccttcttcaa ccatctgttt caaatatctt ttgcccatca ttgttttcga
     6301 taaaaatagt ttggcaaatt caatttgttt tgtgggagca tccttgaaat cgtctatatt
     6361 gcttaacacc cagtcataaa acggtctgtg gtcttcaaat tccaaagtac atattgagtg
     6421 tgtaattcct tctatggcat cctccaaagg atgagcaaag tcatacatgg ggtaaataca
     6481 ccactcatcg ccggtgttgt agtgagattc atgaagaatg cggtaaataa cagggtccct
     6541 catattaata ttgggacttg ccatgtctat ttttgctctt agaacttttt caccgtcttt
     6601 gaatacaccg tttttcattt tttcaaaaag ctctaagttt tcttcaaccg ttctgtttct
     6661 gtaagggctt tccttgcccg gctctgtcaa ggtacctctg tattcacgga tttcatctgc
     6721 cgataaatca tcaacaaagg ctaatccctt ctttatcagt tcaactgcat aatcgtacat
     6781 cttaccaaag taatttgaag cataaaaaat tctctcttcc caatcagggc aaagccattt
     6841 aacatccttg ataattgatt ctatgaatat atcatcttcc tttgtcggat ttgtatcatc
     6901 aaatcttaaa ttgaaaattc cattattctt ttttgccaat ccgtaattca agcatatgga
     6961 tttggcatga cccacatgca gaaatccatt aggctccgga ggaaatcttg tatgtaccct
     7021 attcccgtaa gtgccttttt caaggtcctc attaattatc ttttggatga aattaaccga
     7081 ttccctgttt tcattttcgt tattaaattc gctcattttt tcctcccgca atatatataa
     7141 acaaaataat attattaaac ctaattataa ttaacaaata gctataattc aagtttttct
     7201 tcaatattta ttatatcctg tcttatatac aaataatcat ccggttattt ctttagtttg
     7261 actcaatatc attaaaatac ccggtgacaa caataaacaa aaagaaacgg tcataaccgt
     7321 tcctctttct gccatcataa gcgtaaaagg atctctattt gctgtaatcg tagaatcctt
     7381 tgcctgtttt tcttccaagc agatttgctc ttaccatttt gcgaagtaat ggatgcggtc
     7441 tgtacttagg gtctccgaat tcattataga gaacctccat aatggcaaga caaacatcaa
     7501 gtcctattaa atctccgagt tctagcggtc ccataggatg gtttgcaccc aatttcattg
     7561 cggtgtctat ttcttctgca ctcgcaactc catctgccaa tatacctata ccttcattaa
     7621 ccaatggaat caatactcta tttacaacga atccgggtgc ttcttcaaca cttaccggag
     7681 tttttcctat tttttgagct aattcaaata ccttagcatg aacttcatca tcagtaagtt
     7741 ttcctttgat tatttcaact aatttcataa caggaacagg attgaaaaaa tgcattccta
     7801 ttattctgcc cggatttttt gattttgctg caatttcagt gatggacaat gatgatgtat
     7861 tagttgatag tattgcatct tctttagcta cagcttctat gtcggcaaac attgcatgtt
     7921 tcgttaccat gtcttctgca actgcctcaa tgaataaatc agcatccgcg cattcttcat
     7981 atgtagtggt tttcgtaata tttcccaaaa tttcgtccat cttagactgt tctattttac
     8041 cctttgctac cagcttgctt aatcccttgc ctattacggt ttctaatgtg tcaaatgtat
     8101 tgcctggtct tcctttaact ataacaggat atcctgcctg tgcaaatgtt tgtacaatac
     8161 ccttggccat tgtaccggtt ccgactatgc atatcttttt aatttccatt ttatcctcca
     8221 attatttttt tttgcctgtt ttaattataa cattacgata taaaataaat caagcatttt
     8281 aagaatactt gatgacggtc cggttccctt attattatgg tagattcata ataaccatat
     8341 tatttgttaa taataatgta gggtgaatcg atgattaaaa aatttttaat ttttgcaaca
     8401 aagcttataa ataaaacaaa ggaagacgat attctcgcac tgagtgctca attatcatat
     8461 tatttaatat tgagtatttt cccctttctt attttggcaa tatctttgat gtgcggctat
     8521 agtgaatata tctacagtat cttaaattcc ttatccgatg tgattccgga agaagtgtac
     8581 agaattattt acaatgtgtt aaaatactct gttgcaagct gcagcaagcc ttacttaacc
     8641 atcagcatgt tgataataat atggtcggct acttcgggaa gtgcggctat cataaatgga
     8701 attaatattg catacggatt caataccaaa aagaatttct tattcttaag gataagagga
     8761 attttgttta ctcttgcact gatggtttca atgcaaatta tttttgcggc agttgttgca
     8821 ggaagaactt tattgctctt cgttaaagga atttctattt ttgaggacta taattttata
     8881 tttattgata tcttaagatt tgctgttaca ttttttattg tattaataat tctattagct
     8941 tcatataaat ttttgcccta tgaaaaggtt aaattttcat acgcatatcc gggagcaatt
     9001 tttgcggcag caggctcaat agccggttct tacctctatt cccgctatgt aagcaccaga
     9061 gtcgttttta ttaattccat ttacggaaat ttgtccggat tatttgtatt tattatatgg
     9121 atttatattt tatcgattat atttctgatg ggagctgaag taaactattt tgcagcaaaa
     9181 caaaagaatg gtattaacgg aaaatgacca gaggggacgg ttcttggtgg acagttttca
     9241 aggcgggtgg ataattaatt ctgttggtta tttttttata cacctaaaaa taaaataccc
     9301 tgatttttta tctgctgttt cacagttgcc gaataaactt tcaagttttg ccttggtgct
     9361 tggtgctcct tgtttctttt gaattacaac ataaagctta ccgccatcgt ttaaatgttc
     9421 atatgcacct tcataaaatg aaaatacaac atttttgcct gccctgatag gagggttggt
     9481 cataataata tcataattat ccttaatatt ttcaaatgca gaagaagcaa taacctttat
     9541 tctgtcatca accttgtttt ccttagcatt catccttgac aattcaagtg ctctttcatt
     9601 cacatcgcac atagtaacaa aggatttttt taattttttg gcagcaataa ctcccaaagg
     9661 tccgtatccg cagcctaagt ctaaaatgct gccgctaaaa tcttcatttt catttaaaat
     9721 tgtttctatt aaaagcatgg agccaaaatc cactcctttt ttagaaaaaa cactgttgct
     9781 cgtataaaaa tataaaactt cttttcccac attccattca aatttgtata tttctttctc
     9841 tgttgtcggg ttttcagtaa aatattgttc catttttctc tccttaagtc caaaggggac
     9901 ggttctcccc ggacatcttt atcaactata tatatgatta attattccca atcttcattt
     9961 ctttcattat ttttagtact gtgcctttac ttattcttat tattgcagca atttgtctag
    10021 aagaaagatt gaattcttct ttcaaataaa ttgttaactt atttcttaag ttatggtttt
    10081 ctttttttga taaatcatta atacataatc cctttaacaa taaaatatta tctaaagttt
    10141 ccgtagctct cttttcttgt tccattctca actcttcatt gcttttctct ataaattcaa
    10201 taaatttaat atcacaatct aaactgctaa aatcaataaa ttgtttaatt gctacagtta
    10261 tattatcaga aaaaatattt aatattttat cagttgttaa gctactgctt ttctttgaac
    10321 ttatataatc attgtaacta ctccataaat acttttcagg tgaactaaca atgcctgctg
    10381 ttaccggatt gttgtgtata taccgaacag cggctaacaa ataatcatta ttatctataa
    10441 tttcactaac aaacctatcc tgaaacaaat gtcctactct cttatatttc ttattgaagt
    10501 gatatgcaaa acttacatta attcttttca ttaaccttgt aatattatat tccttatcat
    10561 gcaacaataa atgaacatga ttgctcatta aacaataggc ataaatatct gtgttttctt
    10621 cagaagcttt ttcaaacaga gtagaaataa aacgtcttct gtcatcatca tcaagaaata
    10681 tccttccttt atcgttacca cgcatcatga tatgataaac acctgtatca ctgaatattc
    10741 ttgcttgtct tggcatacta acacactcct taaattatct tcttgtaaca aaaggattat
    10801 gctctgctct gttgtaatta ttgttgttat aaccatatat cattaaaata tttatgtcaa
    10861 cgctaatttg ctaaatattg gaaattaatt aatttacctg cctaatctta aaatagtttt
    10921 aatataaaac ctcatggtgc caaaaggaac cgtccccatt agtcaaaaag aaccgtccac
    10981 gttggtcatc ccaatttgtc attgcttaca catcaaatag gtaaaatgga ttgtcctgat
    11041 ctggatcatt atacatactg caatcttcta accattcatc catattttcc ttaatcgttt
    11101 cagtcagttc ttgataatct tctttcttca ccatataatg ttccaacatg catttataaa
    11161 attttttcaa actggctgct gtactcttta tagttgacgg tgttgaccac atacatttcc
    11221 ttataaagta atctcctaaa aacatatcta catattgata accttgattc attggaatcg
    11281 gttcttcccg aagcaaaaaa tcatttatat aaaaatcaat attgtttaaa tgtgactgaa
    11341 ttgttttttc cgaaagacct ttttcaatca tttccgattg aaatatttca agatattttt
    11401 tattatcatt tctaattttt tcacattctt tttcatattc tacaaaattc ataatttctc
    11461 cttttcaaac aatggaaatg ttatttcatc cttgtttata tgttcttatt aatgtaatta
    11521 tatgctctaa atatatcgct gtaaagatat atagagtaaa tatgcagctt gctttatact
    11581 gaaaataaaa tatttcattt actcatgaag aattggttat caaaaaggaa gaaagaaccg
    11641 ttcctttcag gtcttgaatt tgtccggaga gaaccgtccc cacgtcccca ctggtcacac
    11701 tggtcaaatt atttttcata ataataaaat atatttttta tatcatatta aatatgttat
    11761 aatgaataca atttatgaag caagccgatt ctgcggcttg tactgatgat agaatataca
    11821 ataatttcta aaaggagtga taaatgtgga ctctgaacct ttgtctgata atttaacaag
    11881 aaaacataac aaccctatta aggaagccat gtttatcgcc gcgtcttctt taatgggata
    11941 ggacaataag tctttatcat aaggagggtg caaatggaaa aattagatat tttagaacta
    12001 attgagcaaa aaaaatatat tcagttaaaa aaagaacttt ctgaaatgaa tgaagtagat
    12061 atttcagaaa tattagatcc tttagacctc caaacaacct tgctcgtttt caggatgctt
    12121 ccaaaggata cggctgcaga agtcttttct tatatttcac cggaacaaaa agcagatatt
    12181 attaatgcgg tgacagacaa agaattaaaa tatattttgg atgagctgtt ttttgatgac
    12241 atgatagact taattgaaga aatgcctgct aatgttgtca ataaaatttt aatcaattca
    12301 actcaggaag aaagaacttt aattaatcaa tttttaaaat atcctgctca ttcggcagga
    12361 agcataatga caattgaata tgttgagctt aagaaaaaca tgacagttaa ggaagctctt
    12421 gaacatatta aaaaaacagg gataaacaag gaaacaatat atacatgtta tgttacggac
    12481 aagaaaagaa aattagaagg aatagtttca ttaagaaatc tggtagtttc tgatcctgat
    12541 caaagcatta acgatattat gcaagaagaa gttatttatg tagaaactca cgatgatcag
    12601 gaaataatag caaatatttt taaaaaatac ggttttatgg cattgcctgt tgttgataag
    12661 gagcacagat taataggtat tattacgttt gatgatataa tggatgtaat ggaagatgaa
    12721 gctacagaag acttccaaag aatggcagcc atggtaccat cggatgaagc gtatttggat
    12781 ttaagtgtaa tagcactagc aaaacaaagg attccatggc ttatgatatt aatgcttggg
    12841 gctaccgtta caggcggcat tattacaaaa tttgaaaata tcctcagcac tgttgttgtt
    12901 cttaccgcat ttattccaat gcttatggat accggaggaa actcaggaaa ccaatcttca
    12961 acattaataa ttcgtgggtt agcattagga aatgtgaacc aaaaagacat ttggaaggtt
    13021 ttatggaagg aattcagaat aagcttattg gttggaatta tattagcatc agtaaatttt
    13081 gcaaaaaata tgctgtttga caaggtaggt ttaaatgttt ctttaactgt atccttaact
    13141 gtggcaacta cagttatgtt agctaaaatg gttggcggga cccttccgct tatagcaaaa
    13201 aagcttaaat tagaccctgc cataatggca ggaccattaa ttacaacaat tgtagatgca
    13261 ttggtactct tggtttattt tggagttgca tctgctcttc tgataaaata aaaaagcaaa
    13321 ggaaattaat cttcctctgt ttttaattta ttaatatgca aatatcattc ttccgtattt
    13381 aacagaaacc ggcttcgata aaatccattg atttgttgtt gaatcgtcat agtagaacaa
    13441 tgcaccgttt gtagtatcga ctccgcttag tgcttctttt gcagctttga tacattcttc
    13501 attggcagct cttttaatcc aaccgttttc aacaggggta aattggtaat atccattgat
    13561 gttttggttg attacaccgc ttattgtcgg tgcaaacaaa ccgcttttta ccctgttcat
    13621 aacaacagag ccgactgcaa ccttagcttc ataagactgg ctctctgttt ccgccattat
    13681 taatcttgca agcaagtcca attcttcggc agtatagtct ttatatcctt cgttcggctg
    13741 ttttgcaacc ttaagttctt cttttactgc tgccggtggc aacggaatct ccaaaacctg
    13801 tccaacatat atgtaatcag tccaaatgtt gttggcttgt cttaaattga acagtggaat
    13861 attatatttt tgtgatattt tatataatgt atcacccggc ttcactatgt attttacgtc
    13921 cggaatatta attacttgac cgatacttaa atcatagctt tttaaatcgt ttgcaaccat
    13981 tatatttgat acggttgtgt tgtaagcttt gcttattttg tacaaagaat cacctttagc
    14041 tattgtgtag gttgctgcat atgtatcagc agctacgaac atggatagtc cgatagtcag
    14101 acttgttgtt aataatattg acttaaaaat ttttttcatt ttatcctcct tatgtcttcc
    14161 agaatcctgc ccaggtactc tttaagaaat acggttgtaa aaattgtagt caatatctta
    14221 tatggaatca atggaaagac cttgcaatcg aggatatata acacatgcaa agaaataaat
    14281 tcttttagcc aaaggggtag ataattaaaa taacgttgga attgcaaggc taatttatat
    14341 aaacattttt taacaactaa atattaatgt tttcttaaga ggaatttgtc cagcgagaac
    14401 cgtccccagt gggtaagagg aatttgtcca gcgagaaccg tccccggtgg gtttgnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggtg ggtttgtgac agtacccccg
    14581 tccccttgtc attaaactgc ttttatgcag ttttctttta gctttgcaag gaatgctttt
    14641 tctaccgtgt cgtatacttc gtattctttt tccttgcctt tcagcgtagg tacataaagc
    14701 ttaacttttg cttcgtcctt agcatcctct ttgtattctg catttaacag tccggctaat
    14761 tttgcgaaca tttcaaggtt tgacgtgcct gttaaaggtt ttattgcttg atatacattt
    14821 cttacggtcc cgtctgcgct tactatggta ccattgcttt cagctatgct gcaaagcgga
    14881 agtacatatt cggcattaga ggtagtttca ttttccatta tgtcaaaggc tgcaaagaat
    14941 tttagattgt ctgctgcttt cttaagttca ggaacatttc ccaatatatc ttcgccgatt
    15001 acaactaagc ctttaagctc attgtttaac aatgctttcc tgatttcttc tcccggtgtc
    15061 ctgaaaccaa tattccaagc tccctggctg tttgctttag ctttaagagt tatcaagcct
    15121 ctgtgaggac ggttattatt tcctgctaat acggttatat cagccaggac tttcagtgct
    15181 gcctttggaa ctgtgttttc atccgcaacg ataatgggtt ttctggcctt cttgaaagct
    15241 tctgcaacct tagctgcttc aggaagtact tcggttttgt ccaatgcctt aatcagctct
    15301 tctccgtttt catagcggct tattgcttct ttcttaataa ctccgttgtc aaccaatgct
    15361 ttcagtactc ttgtaaagaa cacttcataa tctgttacgt ctgctgtaac agcaaagtta
    15421 ttaagcttag ttttaatagt tgatgcagaa attattttct tagtgttaga aagtcttctt
    15481 aatttcattc ctgctgcagg gtgatgctcg tacaactgac ctgcagaaat aatcaaatct
    15541 gcattgtcca attcttctgc tcttatggta gattcattct tgcctaatac atattcaata
    15601 ccggattccg aatcaagtgt aaatgaaccc ttgtatttag tatttaattc atctgccaca
    15661 gctttaatat atttaaattc ttcgtttgtt aattttggtg atactacaaa tgctacttgg
    15721 tcatctccgt acatattctg tatgcttctt aagcgtttag ccgtttcaaa taaagcaaca
    15781 ggtaattcta ttggctcata ctttcctttt accttaacct ttgactggag gattctgttt
    15841 tcatcgttaa tatgctcgat accgaattta cccttaccgc atacgggtat ttcattttct
    15901 ttaggacttg atgccaagta aaccatattg tctttgtgct catatacaac atcgcaccct
    15961 actgagcaat agccgcacac tgacttggtt tgtgttaagt ccagaggaat ttccttcata
    16021 ttgcctcttc tgtcaagcca agctcctaca ggacacacat caatacactg gccgcatgaa
    16081 atacagcttg ttacttttaa ttcctgttcg aatgcaggtg ctataaatgt tgcatttcct
    16141 ctttccacaa atcctaatgc tgatgctccc acaacttcgt cgcatgcacg cacacataat
    16201 ccgcaaagta cacatttatc aacattttgc gaaatgaagg gatggtctgt ttctttgaat
    16261 ttcttttcat tttctgcatg atattttttg gtatcaactt caaacttctt gatatagtca
    16321 accaactggc attcaaagta atccttacaa ccgcattcaa gacatctttg tccttctctt
    16381 attgcttctt tctctgtcat tgttggaagt atgggctgga agttatgctt tcttatttcc
    16441 ggctcaacag ttctgtgagg aactcttgga gttctttctt cgtctttata atcttctttc
    16501 gttacttcag tatatactaa tggctgattt ttgtaaggaa tcatttcacc tgccagataa
    16561 cttgaaataa ctgctgccgc acgtccgcct tgtgcaactg cttctatagc aatcttaggt
    16621 cctgttgctg catctcctcc tgcaaacaca ccttttaaat ttgtttcaaa agtgccttca
    16681 tttataacta tatttttctt ttttccctgt cctacattaa ttttaccgca gtcaacttcc
    16741 tgacctattg ccgctataat cgtatctgct tcgaattcta caaattcgcc ggttggaagg
    16801 ggactttgtc ttccggatgc atccttttca ccaaggacca tcttttcgca tttaagtccg
    16861 gttacttttt caccgtcgct taaaaccagt gacggagctg acaagaatga gaatattact
    16921 ccttcttctt tcgcttcttt aatttctatt tcttcggcgg gcatttgttc ttcacttctt
    16981 ctgtaaacta ctcttacttc ttttgctcct aaacgtatgc tggttcttgc aacgtccatg
    17041 gcagtgttgc cgccgcctac aactattact ttttcgccta tttttaccgg cttgttttct
    17101 gctactttta ttaagaaatc aattccgcct aatactcccg gagtattttc cccctcacat
    17161 ctcataggtg aacttttcca tgctcctatg ccaaggaaca ctgcatcgta tttattttgc
    17221 aaatattcca aagaaatgtc ttcgccgact tttacgccgt agttgaaatt tgcacccatt
    17281 ttttcgataa ttgcaacttc tgcatcaact acatttttcg gaagcctgta ttcaggaata
    17341 ccgtatctaa gcattccgcc gggttttggc attgcttcaa atatatctac ttcatgtccg
    17401 tccgccaaca gatagtatgc tgctgacaat cctgcaggac ctgctcccac aacagcaacc
    17461 ttcttgcctg tagcaggctt taattcagga atatagggat tgcctgtatt caagtcgtaa
    17521 tccgctgcaa atgttttaat acacgctatt gataccggct cttccacata ttgtcttcgg
    17581 catgcttttt cacaaggatg agggcatact cttcctatgc atgcaggcaa aggtaatttt
    17641 tcttttatca gttttaatga ttcaatatat tgtttatttg caactaaacc gacatatccc
    17701 tgtacatcag tatttgccgg acagtttagc gtacaaggtg ccttacagtc tcctatatgc
    17761 tgtgaaacca ataaattaag cgcattattc cttgcttcta ttactttttg agtgtctgat
    17821 ctcacaacta atccgtcaga aacgggagtt gcacaagctc ttgcaatttt tggctgtcct
    17881 tcgatttcca cgatgcatag tccgcatcct gcatacactt ccaatctttc atcaaaacac
    17941 aagtgcggaa tttctatgcc attttctaaa gctgcttcca gtactgtttt cccggcttca
    18001 gctgtaagtt cttttccatt tatattaagt tttactaagg ccacggctaa tcctcccttt
    18061 taattttttt gtacagcgcc aaatctgcat acgctgtagc aatttccgca tttaatacat
    18121 aattcttgat ttatttcatg aggattctta acagttcctg aaatacaact tacaggacaa
    18181 tttcttgcac aaagagtgca tcccgtacat ttatccttgt cgatttcaaa agttaacaaa
    18241 gctttgcaat ttttagcggg acatttctta tccaccacgt gtgcaatata ttcatctcta
    18301 aaatatctga gtgttgccaa tacagggttt ggagctgttt gtccaagacc gcagagcgat
    18361 gaattcttta tactgtttgc caagctttcg agttcttcta aatcttccaa ggttccctta
    18421 ccttgagtaa ttctttcaag tatggtgagc attctttttg ttcccactct gcaaggtgta
    18481 catttgccgc atgattcatc aacaatgaaa tccaggaaga atcttgcaat gtcaaccata
    18541 cagttatctt catccattac tatcattccg cctgagccca ttattgagcc tacagctgca
    18601 agattttcat agtcaacggg agtatcaaga tggccggctg tcaagcaacc accggaaggt
    18661 cctcctgttt gaactgcttt aaatgctttc ccgccggggc atcctcctcc gatgtcatat
    18721 attacttctc ttaatgttat tcccatcggt acttcaacca gtccggtatt agtaattttt
    18781 ccgcccaatg caaatacttt tgttccgggt gatttttccg taccaaagct tttaaaccat
    18841 tcaggtcctt ttaatataat ctgcggtatg ttagctaagg tttcaacatt atttattacc
    18901 gtcggttttc cccataagcc tttgttagca gggaatggag gctttacctt cgccataccc
    18961 ctgtttcctt gaattgactg catcagtgct gtttcttctc cgcatacaaa tgcaccggct
    19021 ccaagtcttg tttctatatc aaaatcaaat cctgaatcaa atatattttt acctaataat
    19081 ccaagctctc ttgcttggtc gattgctata tgcagtctgc gaacagctat gggatattct
    19141 gccctgacat aaataaatcc ctttgtagct ccgattgcat aacctgctat agccatggct
    19201 tccaatactg catgagggtc tccctctaaa atacttctgt ccataaatgc accgggatct
    19261 ccttcgtcag cattgcatat tatatatttt tggtcggctt tatttggtgc tgcaaagccc
    19321 cattttaatc ctacagggaa gcctgccccc cctcttcctc gaaggttgga ttctttcatt
    19381 aattgtatta catcttgggg ggacatttca gtcaatactt ttcctaatgc catatagccg
    19441 tctcttgcta tatattcatt aatatcctca ggattaatta aaccgcagtt cctgagtgca
    19501 actctttttt gcttgctgta aaactgaact tccattaagg atttatgctc tttggcatct
    19561 tctaccttta ccttttcttt atataaatat ttttcgacta tccttccctt taacaagtgc
    19621 tcttctacta tttcgtcaac cttgtccaca gtcattcttg aataaaatga gccttccgga
    19681 taaactatga ctacgggacc ttcttcgcaa agaccaaagc agcctgttcc cacaacctgg
    19741 acttctttgt ctaattgtac ttcttttatt ttctcttcaa atctttttct gactacttga
    19801 gatttattag aagtacatcc gcttcccccg catattaaaa catgagctct gaaaattgcc
    19861 atggttttac ctccattttc attaatctaa atttccgaac ctattgtata ttctcttaca
    19921 actcttccat taaccaagtg ctctgcaact attctttttg ctttttcaga gtcaacatga
    19981 atatatgtca ccttttcctt atcaggggaa tatatttcta caatcggctc atacgtacac
    20041 atgcctatac agcctgtttg aacaacttgt acatttttta aatttctttt tgctacttcg
    20101 tctacgaatg tagaaagaac aggtctggca ccagctgaaa ttccgcatgt ggccatacct
    20161 acaaccactc ttatatcttt atcgggattt cgcatttcaa tattttttat tgcttcttct
    20221 cttatttttc ttaattcttc caacgacttc atgctgctcc ctcctattta tattttttta
    20281 gaataccggg gatttttgtt gtatccaatt tgccgtaaac atcatcgtca accatcataa
    20341 ccggggccaa tccgcagcag cccaagcatc gggtagcttc taaggtaaac agcttatcgt
    20401 ccgttgtttt cccgacttta acatttaatt catcagaaat cttgtctaaa atgccctgcg
    20461 agccctttac gtaacaagct gtaccaagac ataatccaat tttgtgttta cccttttctt
    20521 ccgtggaaaa ctgtgtgtaa aaggttacaa ctccataaat atcggccagt ggtatatttg
    20581 tttcatctga tataaactgt tgaacttcaa gaggcaagta tccaaataaa ctttgtgctt
    20641 tctgcaatat ttgcatggta acgccttgtg agttttttac cgaatcgata tactctttca
    20701 attcggcaaa ttcacttttt tttgattgta aaaactcatt ttgcaatttg ccatcctcca
    20761 atttatttta aaaataacta taacattaat tatacaatta tcaacataaa aagtcaaaat
    20821 attgttgatt tttatttaag gttttgataa tttataagca aaagaatcct cactatgagg
    20881 attcttttgt attaaaatac ttgttcatta tttttgaaat attaaattat tctgctgtat
    20941 tgtttaatac cgttaatttt gccaaatatg attgcccgcc atcttctatg catttaattt
    21001 gataatttct aatcaaatta aattcaccgt catatcctct cataagccaa ttcaggtcag
    21061 catttaccaa atatccgtca tcggtaagct ctatatcata aatttcatta accttaacct
    21121 tcattgccaa ttcataatct gtagggctgt tctttacctt atataaaata tccaagtcat
    21181 tttgcagtaa attatcggat tcaattttgc ttaacttctc ctccaagctt tgcatatctt
    21241 tatatccgta gagaaattca ttcattattg ttatccgctc atttagcaaa tactcaatag
    21301 tctctatttc tttatgatat tcatagccgt taattgaatt gtaactgaaa agcagcaaca
    21361 cgatcatcgt gaaaataaat ctctttttca tttgtaatcc ccctgcaata aagatgtctt
    21421 ttcattatag gggtattact ttgattattt tatcaataat tgtcgcgctc aaaattaaat
    21481 tactcgttta atttcaacaa actttgtatt atttattcaa attttttaca ctttttcttt
    21541 tttctatggt aaatggcagc tccatacgtc cttgttgaat tttatcgcct tttatcattt
    21601 ttataagcat tctcatacct accgctccaa catcataata aggctcacct attgtagtca
    21661 gttcaggtct cacgaatttt ccctccctaa tatttccaaa tcctacaata gacacatcgt
    21721 ccggaacttt aattccgttg tctaaaaagc tgtccaatat ccctattgct atttcatcag
    21781 agctgcaaac acatgcagta aaatcctttg cttcatcaat tattgatttc cctaaatcat
    21841 atgcttcatt gtgcttgctt cctccgacaa catatatttt agaatattcc atttcattat
    21901 cttcaattgc ttttaagtat ccttcaacct tttcgtcttc tacggtagtt ctgttttcat
    21961 agtcggaaaa atatgcaatt tttctgtggc cttcttcgaa taaatatttt gttacttcat
    22021 aaattgcaga tttgcagtca attgttatat aattcatgtt ttcgcgaata tctcttgtaa
    22081 aaaatacgca gggtttatta aagcttttag ccaatttaat aatttcttca tcaaatatac
    22141 ttcctacaag gaacaaacct tcggcttgct ttctgtctag cagctgcaga tatttttcct
    22201 gagtttcctt gctgccatag ctgctgcaaa gaagaatatc atatccgtac atttttccta
    22261 tttcttcaat tcctctcaca gtgtctgcaa cgtagctgtt ggctaaatta ttcactataa
    22321 cacctattaa atatgatctt cttgttacaa ggcttctcgc aacatcattg ggcttatatc
    22381 cggtttcttc aataactttt aaaaccctct tcctaacttc gggactaaca ggctttgagt
    22441 cgtttatcac tcttgatacg gttgaaatcg atacctctgc aagcctggcg acgtctttga
    22501 tcgttatcat atattcctcc tataatcatt ggtaatgtat tcctattttg ttaccattat
    22561 atatgaattt catgataaag gcaaatgtta ttttcccgtt ttcctaaaaa aatgtcatcc
    22621 tgagcggcag taaaaatctc agtatgcagt gagattctac gtgcccctct cagaatgaca
    22681 aattaaagta tgacaaatct atatttattc ataacataat aatacccgca atgccgcaaa
    22741 cctataattc acacccgcac ctaggcaggt gggatttctg ttggctgatt ctggcgtccc
    22801 gtcaaagcgg gcttgtcata ggcagtcaaa cgcgaattcc cttttacaat atgtcggttg
    22861 aattaaaaga tttccgaaca ccgcaggcta tctttaattt aattatatca aatttttaga
    22921 ataattacaa ccggttttta tgaaggctgc tttattcact agtattttaa cctgtgtttt
    22981 agataatatt caactatatt tttaaatcaa attatccaat tcatcctggt aaataacttc
    23041 tttttccatt aacagtcttg ctattttctc aactttttcc ttattgcatt caattaacct
    23101 aatcacctct tcatatattt ctgaagtcaa tttttttacc gagacttcaa ctgatgtcaa
    23161 tggtattctc agtttatcgg acagcagctg ataatttatg aatgttgcat cattcatgcc
    23221 gtattttgaa acataatcca ctgccaaaga ggttactttt tcaatgtcat tttgtgctcc
    23281 ggcggtaata ttttcattcc cgaagaatac ttcttcagat gctctccccg ccattagaac
    23341 agataaatga tttatcattt tccctttggt cattacctct tccgagtcag gagtgctcag
    23401 cgtgtatcct ccggctccct tactggtggg aattacagaa actcgaataa ttttattttc
    23461 aggcagcaaa tgtcttgaaa caaatgcatg tcctgcttcg tgatatgatg taatggtttt
    23521 ttgaaatcca ttatcataat cctctttttc aactcccgcc aatatttgca aatacgcttt
    23581 tttgatatgt tcttccttaa ttttatctga attttgtctt attgccaaaa gtgctgcttc
    23641 atttattaaa ttttcaatca ttgctccgct gaaataaaca gtcaaatcag ccagctcgtc
    23701 tatattaata tcttctgcat tttttttgtc caaataaagc tttataatat cttttcttgc
    23761 atttttgtcc ggaagcatta tttcaatttg tctgtcaaat cttcccggtc ttaacaatgc
    23821 actgtccaaa gtatcaattc tgtttgtggc tgcaattact acagttcctg aatcatcggc
    23881 aaaacccgac atctcggtta gaagtgcatt taaggtcctg tcaccttcat cgcttccttt
    23941 aatgctacct gacattcttt tctttcctat ggcatctatt tcatctataa aaataacact
    24001 tttgcctgat tttttagctt ttttgaataa atttcttatt cttccggctc caagacctgc
    24061 ataaacttgc acaaaatcag aacccgatac tgcatagaaa ggtaccccgg cttcacctgc
    24121 caaggccttt gccattaaag tcttccctgt tcccggttcg ccgtaaagaa gtaccccttt
    24181 tggcatcctt gcattaaact tgctgtattt ttcaggattt tgtatgaagt ctaccagttc
    24241 aagaagactt tgttttgctt catagtttcc ggccacatca tcaaatttca tagttgatga
    24301 gcctatatat tcaatttcat ttaagctgct tatttctttt tgtgcagagc gtcgattttt
    24361 aaaaaatatg aaatatataa aaattataac gccaataatt gatataatct gatatgtttc
    24421 cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatattgaa gcgtgtccca
    24541 taattatttt tttctttatt aaattataac acggggatat acttttatat atactcaata
    24601 aatagcttgg attcattatt tttcctttgt ttccaacaaa catatgcact ctgccccaat
    24661 accctcgccc cttcctataa agcccagggt ttcggtggtg gttgccttaa tgtttatact
    24721 agaaatatca gtttttaaat cactcgcgat attacgcctc atttcatcaa taaatggaga
    24781 taatttgggg ttttgagcaa taataacggc atctatatta ccgaccgtaa atttattttc
    24841 cctcattatt tcatatactt ttcttaataa aacgcgactg tctgcatcct tatacttagc
    24901 atcatcatca ggaaaaagcc ttcctatgtc tccttttccc atggctccca aaaggctgtc
    24961 cataattgca tgaattaaaa catctgcatc ggagtgaccg tcaagacctt tttcataagg
    25021 gattttaacc cctcctatta ttaatttcct gttttcagcc agagcatgta catcgtatcc
    25081 tattcctatt ttcatagcag ctcctttaaa aaaagttaac aataatttct gccattctca
    25141 aatcgagagg tgtggttatt ttaatattct tatataatcc ttcaattgtt ttcactttat
    25201 aaccgtagta ttccacaatg gatgcatcat ccgtaaattc tttttcttca gtcatggctt
    25261 tttcatagca ttccctgatt atgtcataat caaaagcctg aggtgtctgt acagccctca
    25321 atttttttct gtcaaatgtt tttacaacca ttccgtcttc cgatacttct tttattgtgt
    25381 ccataacgtc tatcaccggt acgcaagctt tatgaattat agcattttca acgcatgtgc
    25441 ttattaaatt ataggaaacg aagggtctta ctccatcgtg gattaagact atattacagt
    25501 ttttactgac cttttttatg ccctgataaa ccgattcagt ccttgttttc ccgcccggta
    25561 ccacatatgc tattttacgg taaaaaggag cgatttcatc tttaacgaac tctatatcct
    25621 ccttattaac aaccagaata atatcatcta catcattgct tttttcaaat ttatacaagg
    25681 tggtatccaa aattgttttt cctgcaatat ccatatattg ctttggaata tcagagtcca
    25741 tcctttttcc ttttccggct gcaactatta ttgcagaaat atgttttttg ttttgcatat
    25801 cttcctccaa acggggcatt ggctaatcat aataattttt taatattcaa atcatccaaa
    25861 gaaacatcgg gaactcctaa tttcctggaa ggtataaagc ttccgtggca atcggagccg
    25921 cctgatatca ttaaattgtt ttttttgcaa aaatcaatat aatatcgggt attgtccctg
    25981 ttataaggag aataacactc aattccgtct attccaagct ccccccagtg ttttaattct
    26041 tctatgggca taacagaatc cctatagtga taggatggat gtgcaaggaa tattttccct
    26101 ccgcttttct tcgctatgtt tattacttcc tccgggcttt tcaattcagc cttaagtccc
    26161 gattcataaa catccttcaa gtgttcgggg atatctttgt gtattccttt atcaatcatg
    26221 tagttggctg acttccagcc ccctcttttt ctgtcattcg catatctttc gaaatcttca
    26281 acactaatat ttttatattt atttgcagca taatttatat aatcaacatt tgagtttaat
    26341 ttgttttgat ttgtccactt cattaactca agcagttctt tgttttttat atcataattg
    26401 tataaattca aatgatattc tttcccttta tattcagcgg atagttcggt tccgggaata
    26461 aaaattaaat tgtccttatt ttttaatata ttttgcatat tttcgatgtt ttcaacactg
    26521 tcatggtccg taacggaaaa tatgtcaatt ttattttttt tcaattcatt ttttaattct
    26581 gtcacatccc aagtgccatc agaagcattg gtgtgtatgt gcaagtcaac cttcttcata
    26641 tttgtcttgt ttgatatagc aagcagctac catatattcc tttcttagta tagagaatcc
    26701 tcgtgtattc tggtataaaa cattgttttt acttcttcaa aatctttggt ttgagccata
    26761 atccacatca atttcgtaat tacagcctca atggtcatgt cataagcttg cagaacatta
    26821 tatttttgaa gtgcctttac tcctacctca tataagccca tgtcgcttcc ttcaaacatt
    26881 acctgggtag caacaacaat tattttccct ttttttataa gttcttcaag tttatttaaa
    26941 aaattacgtt tgtcaagaaa agggagtcct cccgcaccat agctttcaat tactattcct
    27001 tcatatttat tgcctataaa atcaagaaca tccggctcca ttcccggagc taatttcaat
    27061 aaaaatatgt ttgtgtaaat ttccttataa aactttactt ttttctctaa attataatta
    27121 cttatatatt ttgtaatacg cctgtcatct attattgccg caaccggaaa attaatactt
    27181 tcaaaagcac tgtaactttt tgattttact tttctcgccc ttgttccgat tatcgctttg
    27241 ccgtcaaata ccacatatac accgcttatg ccctcttccg taacaaacct gaagctgtca
    27301 aggagatttt tctttgcatc ggttatatca acatttatgg gtttttgaga gcctgttaaa
    27361 acgattggct tgcatggatt ttgaattaaa taagaaagag ctgctgctgt atatgccatt
    27421 gtatctgtac cgtgagacac tataaaacca tcatatttgt cataattttc ctctatcgtt
    27481 tcagtcatca tgacccagtg ttccggttgt atattagtgc tgtcaatatt taaaagctga
    27541 attgtatcca catagcaata tttctttatt tcaggcacat aacttaaaag ttcatctgag
    27601 cttatagcag gtgccagacc gtgttcggtt atctttgaag caattgtacc ccctgtcgct
    27661 atcattaaaa tctttttcat attccctcct accttccttc aattaccctt ttcatatctt
    27721 ccggcatagg acatgttatt attagctctt tatttgtaat cggatgctta aacttcattt
    27781 catagcagtg tagtgcctgc cggtctatta ataagctttc atttccatac aatgtatcac
    27841 caacaatagg atgacctata tgctttaagt gaacccttat ttggtgtgtt cttccggtta
    27901 taagcttcag cttaaccaat gtcatattat tgtatctctt ttctgctttg tattcagtaa
    27961 tgcattcttt tcctgaagta tgaactgtcc ggattatatc ttcaggatta agtctggtaa
    28021 ttggaacgtc tattcttcct tcgtcttctt ctagcaaccc ttccactatt gcatagtaat
    28081 atttttcaac catgcttgac ttcatctcga gcaccatctg attgtggata tatggatttt
    28141 tggctattaa aacaatacct gatgtgttca tatcaagcct gtttataaat ctaacctttc
    28201 gtttaatatt gttctttttg aaataatatg caacaccgtt tgcaagagtg tcgttttgat
    28261 gagatcttgt cgggtgaaca accatatgcg gctctttatt gacaacaagc aaatcatcat
    28321 cctcataaat aatgtctaaa ggcatatcca cagcatcata cctgtcttct tcctcgtcaa
    28381 actcaatcga taattcatca cctgcaaaga tactcttgtt taatttcaca gctttcccgt
    28441 ttaacagaat tttgccgtta cgctctaatt ttcttgagag tctaccggag aattttaatt
    28501 tctctgtcaa tatgctcttt acagtttcat cattttcttg tattgtatat ttaagtatat
    28561 tgttaaacat tatgtactcc ttggagtttt tttatgacga ggactatgct ctcacagcta
    28621 tgacgagggt tgacctctta gattatatct tgtgttctta taataatata taagattaat
    28681 aaagtaaagg ggataataat gaaggaaaaa gtaataaata ttgctgctaa cgatgatgtt
    28741 tatacagctg atattaaaaa aaggctcatt aaagttttaa ccgaaaacgg ctatgattat
    28801 tacgaaggtt tcaaggaaga cgcacaatta aacattactg tgggagggga cggagctttt
    28861 ctaagaagcg tgagagaaag taatttttcc caaataccct ttataggagt aaataccgga
    28921 accttgggtt tttatccgga aattacacct gaaaaccttg aaaactttat aattaattac
    28981 agagcaggca attacaaaat aaatcccgta aatttaattg aatgtgaaat tgtttccgca
    29041 aatttatcca ataaaattta tgctgttaat gatatcatac ttaaaagaat agatatgaaa
    29101 acactgcatc taaatgtgtt tattgcctca aaccacctgg aaaaaataag cggcgacggt
    29161 ttaataattt cctccccctt aggaagctcc gcatataata agtcagcagg aggaagtttg
    29221 gtgtatcctt cattaaaaac tttgcaaata acgcccttat caccaatttt gtcaaatgca
    29281 tacagatgtt tggaatgcag cataattgtt ccccccgaat ttgaaattgc aatttatccg
    29341 gaaagaagag aggattactg tctagctatt ctagcagatg gggaaattta tgaatacgat
    29401 gatatggaac atgtgctttt ccgcacatca aagaaggtaa ttaaccgtct gtcaacggga
    29461 acatttaact actggaatgt tataaaggag aaattccttt aaaggaattt ctcctgcttt
    29521 attacatcct taatataaac ttgcttccta cattaatggt gctttctacc gttaatgaat
    29581 acttgtgctt gtcggcaatc catttagcaa tgctcaatcc aagccctaca ccgccttccc
    29641 tgtgccttga cttgtctgct ctatagaacc tgtcaaatat tctttccaaa tctttttcgt
    29701 tgatgccgat tccggtatct tcaacaatca atactttttt gtttttatct gtttttaaac
    29761 ttaccgttac tttgcctttt tctgtatact taactgcatt gtcaattaaa attacagcca
    29821 atctacgcat cttatcataa tctgccttga ttattatatt gtcttcaata tttgttatga
    29881 gctcaatatt cttttttgca gcaatcactt ccatggattg gcatacattt tctataactt
    29941 ccgaaaaaga aaactcggaa atatctaatt cttcttcatt gttatcagcc ttggcaagaa
    30001 ttaaaagctg gtcgataagt ctggccataa cccttgtttc ggaatatgca ttgtcaagcc
    30061 aaatttcatt ttcatcaaca gtgccgtcct catcgcttaa aacaacatcc aagtttgttt
    30121 gtattacggt aagaggagtt ctgagttcat gagatgcatc tgccacaaat tctctttgtc
    30181 ttttccatgt ttcttttatg ggagctaagg ccttgcctgt aagcatatag cttaatacca
    30241 gtaatattac ggtgcccgta attcctatca ttatcagtac cgttcttaag aacgaccata
    30301 atatatcctc gtttacggtg ttttgataaa cctgcactac gctttgcccg gtataatcac
    30361 taaaatatct tgtataaatc ctatagttaa aacgacctat tttttgtgtt tcatatgaat
    30421 cttttttttc ttcaaaagct ttttgtgcaa gcggcttaat atggtctgct atttttattt
    30481 caccttcctc ggaaagaaca ggtatcatat attggtcata aacaacataa catgcatcaa
    30541 gcctcctgtt ttccgaagga ttaatttgta tatacctttt aaataaagtt ccgttaaaga
    30601 agtattgcgt ataggcagtc tttttatagt tataaattct cagggcttct gctctcatca
    30661 gctctttatt gccgtcttca aataccattt ttgtgaattg atatattcct gcggacacaa
    30721 aaaacataaa aagaataagt ataatgccca tgtatgctgt taatttcaaa tgaagttttc
    30781 taaacattat ttctccttca gcatataacc tacgcctctt acagtttcaa tttttacatt
    30841 ggtattttca agtctctttc ttaaatgatg aacataaatt tcaatattgc tttcaagtat
    30901 atctgcttca agtccccata ttctatcaat aatctgctct ctcgtaaaaa cctgtcgagg
    30961 tttttttatg aacatttcca taagcattgc ttctttaaga ggtattttat attcattgtc
    31021 tcctacatac agaagataat ttttaacatc gtacttaata tcctcaaatt catatatttc
    31081 accaacaaat tctgtgctct ttcttcttgc cagcgccttg actctggcaa tcaactcctt
    31141 tgttgcaaaa ggtttgacaa gataatcatc ggctccggat tccagtccga ataccctgtc
    31201 atccaccgta tccttggcgg taagcatgat tacgggtgtt tttattccat cttttctgat
    31261 tgtttttaat atttgaatgc catctactcc cggaagcatt atatctaaga ctattacatc
    31321 ataaatatcc ttgcttgcaa gaatcagtcc ttcttcaccg tcatttgcaa tgtccacatc
    31381 aattttttgc ttattgaaca aataactcag tgcctgtgtg attttaactt catcttcaac
    31441 gattaatatc ctcaatacaa cacctccaat aatatgacac tcgtcatcct aagccaagtt
    31501 tggttacccg acaattcttc gaatttcatt tttagttatg actatattat accaaaaacc
    31561 ttaaaattac attaatatta tgtatatcat agatgaataa atcaaatgtt gtcaagaaaa
    31621 aaggatgcat gagtctgcat cctttttgaa tatatttaac ggttactgca gcaactgcag
    31681 tacaccctgc ggctgcatat ttgcctgtgc aagcatagcc tgagcagctt gtgtaagaat
    31741 gttattctta gtgtaagcca tcatttcttt agccatgtca acgtctctga tacgagattc
    31801 agcagctgtt aagttttcgg tagtaactcc caagttgttg attgtatgct ctaatctgtt
    31861 ttgcatagca ccaagacctg ctcttgctgc tgatacgtca gctattgctg cgtcaatagc
    31921 atcaattgca cctaatgggt cacctgtagc tgtaacatca atagtatcta cacctaagtc
    31981 tgatgctttc atactggcaa tagttaaatt aacagattca tagtccgcgt tatttgcacc
    32041 aatttggaaa tctataccat ctgatgccat gctgccaccg tttagtaaat cgatgccgtt
    32101 gaattttgtt gaatcgccta ttctgtcaat ttcatctgct aaagcatcta tttcgtcttg
    32161 aatgctttgt aagtcttctg cttggttcgt tccgtttgct gcctgaacag ccagttctct
    32221 cattctttga agaatagcgt gagtttcgtt taatccgcct tctgctgtct ggattaaaga
    32281 aataccatct tcagcatttt tggtagccat ctcgagacca ttaatttgtg ctctcatttt
    32341 ttcagatata gccaagcccg cagcatcatc accggctctg ttgattctaa agcctgatga
    32401 taatttttct aagtttttac ctgtgttagt gttatttgtt cctaattgtc gcaaagagtt
    32461 cattgcgttt aaattgtgtt gaattctcat ttttttacct ccgtgtaatt ttcttgggat
    32521 ccttccctat attttacata ttctattttt cacaccggcg cctcagtata aaaaatattt
    32581 ttatgtttac ataacatata tcgtccacct aataaatata tttagtgttt tataaaaata
    32641 tttttagatg ttattaattt tttttagttc ctcatcatta aaaagcttcc taaaaaattc
    32701 aaccaatatt atgttatcaa caattttact agctttgaac aagtagattt tatccttaaa
    32761 taaattataa tcataaatct tgtttaaaaa agctactata ttttcttctt tctcttgtgt
    32821 taaaaaggta tttagtaaaa ttattaaaac ctgtatgttg tatttattaa cctttaatat
    32881 agaaaccgca gtctttacaa tcttatgttt attgttttga tgattatatg cattaagcaa
    32941 ttctatatac acatcatcaa ttttccctaa agctaaactt tctgcattac aattatattt
    33001 ctcgcatagc ttaatacatt tttccaataa gtttacagct tcttcgtatt tatttcctct
    33061 tacggctgca atacctaata gccagtaata atcagggtat ccggggtatt tttttatagc
    33121 gtcatcaaaa ataactttaa tctcatcata agattttaat aaagataaca tatttatcat
    33181 tataccgcta taaatagtac aataataatc aaattcatag aagtcatcta tatagctgat
    33241 agcttttaaa ccatattcaa tagctttttc atattttcct tcaatgttca tttgcctgct
    33301 catatagtaa ttaattttaa tatctttggg attatttttt aactcccttt ctaataactg
    33361 aaaattccgt tcattcttgt ttttttcatt cacaatcgtt ttatcatacc cggtgtgata
    33421 aatttttagc aaatcactaa tatctgcacg tacaatatta tcgtctgttt tattaacatt
    33481 taccaatact tcatggattt tattcttata tctgagatag ctttttcttt tgaatattct
    33541 tgcacttaca tttttgactg cagagattat ttcaccattg gattttatat taataaaatt
    33601 agttgttaat ccttctattt ccttttgtct gccaaatttt tttatacatt ttcttattag
    33661 cggaacagaa tcttcagaaa agtattcgtc acaatctaaa aatattatcc agtccccttt
    33721 cgctttatct aatgcaaagt ttctcgcggc gctaaaatca tttatccagt caaaatgaaa
    33781 cacttttgca cccattattt ctgccagctc tacggtccta tccgttgaac ctgtgtctat
    33841 gactatttgc tcatccacaa ccgttttaag atggcttaga caatgttcta tgttagcttc
    33901 ttcatttttt gctattaaac attgtgttat atacataacc tgctcccttt cttaatccta
    33961 aagtatatta ataatttggc attgctcttt ttctatataa tggtatttta tcagctaata
    34021 tttttttaat attttgttca ttgttcatta attcttcgaa cattgatacc atacagcaat
    34081 cattatgaaa ttcctcaatt ggcaaaacaa aatcctcaag ccctaaatct ttagctattc
    34141 cttttgattt taccgaatat ccgataacca tagacggaac aaaggtagaa tatgccgcta
    34201 tagaagcatg tgtcctggca aacacaccaa gacggcaatt tgaaataaca tacttatatt
    34261 cggctgcact tatattttta tttacaaaac aaatacgccc tgtatcctta aatctgttaa
    34321 atattttttc taatatttca aggtcattat ccataggcat taaaacatgc gggattaatg
    34381 caatattcat atctgttttg ttaataacca aatttatcag ttttattaca ttctttataa
    34441 ctattccttc cgtcttttcc cttcttatga ggagtggact aatgttaatt cctacagtat
    34501 tacctacaaa aaaattttcc ggaagggaat attcctttgg ctctaataag aaggctacat
    34561 ctgaacaaag cctgatgttg tcaagacctc tatcttttag tgcattatat gtaatagatt
    34621 ctcttgcagt aattagatga tgcgatttta ggacatcgag catttcatta tccatcattg
    34681 acggctctat agagcagctc cataaaatac ttcttgcacc ttcttcaatt gctctttcat
    34741 gaattgcttt ccatttcctc cagttgtcat agcaataatt atcaccgcct acagataaac
    34801 aaaccgtatc ttctgtaatt tgctcaaggg tggatttata gataaatcta tcataaattc
    34861 ctttttctga ggaatttttg tccaattcaa catacttcat atcacgttca caatattcat
    34921 cgattggcat attaaattca atatcttgtt ctttaaaatg agttgatagg ataaccttgc
    34981 tgtccggatg tctcttttta atcacgtcta cagtagattt aacaattgct tccgccccat
    35041 gattatatgc accgccatgc ccatataata aatacttttt catataccca acctctcttt
    35101 aagattttta aaatctatgt cgtcagataa aatctcatcc caatcagcaa actcttttat
    35161 tgcttcatta gttttagaaa gttcaataag caaggatatt tgtagtttct taaaatatat
    35221 aaaataatca tgtgattcta atttagcaga caagtttaag gccagcaaca tcaaagactc
    35281 tacatattca tcagggattt ctgttccttc tgcaattaat tctataattt gactaagtgc
    35341 attttcttca tcattttgca gaatagaaaa aacaatatcc ttaaaataac ataaaaatcc
    35401 tacttctaat ccgacaagat aaggtgaact gcttagcatc cttgatgctg cctgactaaa
    35461 taaccggttt ttctccgctt ttgctcccgg ccatttgata agctcttgag tatgacggct
    35521 ttgaaatata tcgagcaaat attcaaattt atcagatacg cttatttgag catttagtaa
    35581 aacatttaac gtatccttga ttgcattgaa atatacaatc catagaaaat taagattttc
    35641 atcactgact gctccacatt ttgaaatcaa aaaatcacaa gcagcatcaa aaagtacacg
    35701 atcagatttt gtacgatttt tatcaaattg ataagaaaca gattttggag aaacataata
    35761 tttatgtaat aattccgata aaattccaac acggttggcg ttataaaatg cattcattgc
    35821 aaaaagcgta tcattgccat aacccggttg tttatatctt ttatactcag gcctacgtaa
    35881 tactgatata gaatacactt ttccccaaat tgtccgcata aattgatgat attttggaaa
    35941 atatctatca aattcttcct cgtctaaaat tagatttttt tcaagcaatc tcatcccaag
    36001 atgtttattt gtttgcatat caacaaaatc actgccacaa gctgcaatat ctaagttttc
    36061 atttttaata aaaaaaagca ttttttcaag aaaatttggt gtgtattcat catcggcatc
    36121 aagctcgcac atataacagt cattttcatg attttttata atatctaaaa ttccatttcc
    36181 gacccaattt atttcattat gtaatggaat aattcgctta tcatttttgg catattcttt
    36241 tataatctca tatgtgccat ctgttgaagc attgtcaaga aggtagtata taaattcttt
    36301 atgtgtttga ttcagaatac tgtcaactgc acgacggagc gttttttccg cattatatgc
    36361 cggtgtatat ataataattt tactcatctt acaactacct ttcattttat gtctttcttt
    36421 aattcagata ttcatggctg ttataatttc ctctacagtt tttcgtaata atccccagcc
    36481 atcttgtgta tagagccatg attctatttc acgcaggaaa tctaacttat tagaatatat
    36541 accgacttca tcccaatgct ctaaaacata cttcgtcata tcatctgtaa aaatattatg
    36601 caaacttttg agtttttccg ataatttaac atctgcctgc tgtattttag ggagaatata
    36661 ttttatcaaa attaaaaata taacacacaa ataattctca ttttgcttac ttatttcacc
    36721 gtagtcaaga agatactctc tactgatttt atgtaaatat ttacaagcat aaaaccagtc
    36781 cggattatat ttatatgaca ctgtccttgg tgaaatatag tatttatgca gactctcgct
    36841 aagaacaccc gcccgtttag ctcgtcggaa tgtctccatg caaaatgcgg tatcagaaaa
    36901 attcatggca ttgtgatacc attcaaaatc acatttcttg agaagttcaa gagaataaat
    36961 acccccccat aatgtgacta taaagttacg gtaaagcgga aatttatctg caaattcttt
    37021 accttcaaga attaagttct tattaagtac ttttttcttg attgtttgtc ctgttttttc
    37081 atctatccat tctgttccgc aagtagcaac ttcaagatta ttatcctcca caaaatctat
    37141 catcttttct agaaaatccg gtgagtattc atcatcagcg tcaagggttg caaaatacga
    37201 gttctttagc atattatctc ctgaaattat atccttgatt attttaggaa ttactctgcg
    37261 acaattagat tccccaaaca gtggtataat cctattatct ttattagcat attccttgat
    37321 tattttcctt gtatcatcaa gagaaccatt atcaaaaaga taataacaaa aatcggtata
    37381 tgtttgatta agtatgctgt taactgcttt atgcaaagtc ttttccgcat tatatgccgt
    37441 tgtaaaaata tataccatat attcccccta taattgttca gccggaacaa atcttacaga
    37501 atcatcacac attccattac aataagcaca agcacttaga tattttatgt caaaaagctt
    37561 cattattttt tccttttgtt gttcaatcgt gttatcatca aataaatcaa tatactcatc
    37621 cttatttcgt tcaattaaat tcaactccat tattctgtgt gagaaagtac atggatgcat
    37681 ttctccgtct ttaattgcca aacaataatt caacttactt ggaatagcgc attttttaaa
    37741 tttttccatc atctgagata ctgaaaataa tttttttgaa aaatcactaa aatcaaccca
    37801 tcctccacaa tgagcatttt ctccgtaata aatccgtatc ttgtgattaa tacccttctc
    37861 aataaaatat agtccaagct catgtatttt ttttgatagc tcaccatagt ggtctaccaa
    37921 caatgtaatg caaccatcct caactgcata tttctccaac acagccttta gcttttcatt
    37981 tggaaataag gttccattcg ttataatttc gattttccca aattgttttc tatattttat
    38041 tgtctcaatc aaaattttat ccaaatcttc atgcaaaaaa ggttctccac ctgtaatagt
    38101 aaacttttct acataatcaa caatagaaaa ataccttctt aaaatctcag acagcctgtt
    38161 gatactgtaa tgaggttgtg gattataata aggtgaatat ccagcacata atttacactt
    38221 taaagtacat ttctgagtaa tggtaaggcc actatgatat aattcaactc ttttattcac
    38281 aataatttac tcctaatcgt aatcaataat tttctgtata atatctacaa catcagtttc
    38341 tttcctcaaa gccttaaata acaattcacg tttattattc aaagtaacag aattgagtat
    38401 acatgggttt tttaatgtag catccttaag tgtcttttgt tctttgctta tatatttact
    38461 tatttcttct aagtaatgct tgcctttatt ggaattaatg agtaaaagag aaaccccttt
    38521 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngattgacaa ggtgttcccg
    38641 taaacattac agtcccatct tcttctagca gcttttttat gtccctaaaa attaatccta
    38701 ttttgctttg aacatatttt gactgtctta gctcatgtag ctcttctctg ctatttatta
    38761 gacaatgttc aactaagtta tttgttttat atctggcccc tgaaacaaaa cctccattat
    38821 caagcacctt gtgagctaat tccgtaaata ttcctccgga tgaactggaa gcccttactt
    38881 catcatcaag actccatgca gcatatactt ccggctcgat aaaattatca tttacaggat
    38941 tattgattgc cggacatgat ttttcgcaca aaccgcactc cgtacatttc tttatatcaa
    39001 tttcaggata taaaaagcct tcatcatcat attccatgaa aatgcaattg tttggacata
    39061 catttgcaca tgcggcacaa cccgtacagc aactcttttc caatgcccta gaaaaattaa
    39121 tattattcat cctagaatta ctccgtcttt gattatttga catacctctc catccacact
    39181 ttttcgaact ccaaaatata tttgccataa taaaaatcat cccaatgtgc tcgtaaatta
    39241 tcacacataa gcactagtaa atcagcacaa ttgttgagac catatctatg aatcttctct
    39301 accattgaaa tcatataaga ccattcgctg taaagagcca aacccgctaa ctggggtaat
    39361 attttgctta tatattcggt tcgttcgcga aacgcagcta agtattcatt caattgaacc
    39421 ggattgagca aatggtgctt tgtcgctgca cttgagttgt taccggagtg gcgataaaaa
    39481 caatatctgg gaagtccatg tgctgctaca atttttgcat tagcaaaata acgataggtt
    39541 gtggttatat cgtcatattt gccttcgttc aaaaaacgaa tactatcaaa catctctcgt
    39601 cgaacaagct tagtcggcat ggctgcatta taaagcttac gccataaaaa gtttaccgtt
    39661 gcttgttccg catccatgat atatttctca tcatattgat aaatgccatt tggtaattgt
    39721 tgaccatttt cctcttttgt tgaaccacat acagcaatat ccgcattatg ttttttggca
    39781 agattatata aaaattctaa aaagtcagat tcagcatagt catcatcatc tataaaggct
    39841 acatactctc ctgtggcagc atccaaacct gcatttcgcc cacttccaat atttccttgt
    39901 tctctgtgga ttatcttaat tcgtgaatct tttaatgcat attcgtcagc aattgctcca
    39961 cttctgtcag tggagccgtt atcaacaatt ataaactcaa aatctttaaa agtctgattt
    40021 aatatactgt caattgctct tgttaaaagc tcttcacgat tgtaggtaag cataataaca
    40081 ctaattttca tatctaccat accttccaag gtgccatatt ttttccccac atcgattcaa
    40141 ggtgatactt atccctctgt gtatccatgc actgccagaa tccataatgc ttgtacactc
    40201 caagcttctc ctcggcactt aaactttcaa ggggctctct ttcaaaaatt gttttatctt
    40261 cttttatata attaaataca gctggctcca aaaccataaa tcctgcattt atccatcctg
    40321 cgtcttcagt cgccttttct ctaaatccta taaccttcct gtcattttca tctatatcca
    40381 aaacaccaaa tctgccaccg ggctgtatgg cggataaagt aacaatatta ttagtcgatt
    40441 tatgttgttt cactagttta tgtatgtcta tattgctaac tccatcgcca tatgtcatca
    40501 taaaagtttc atcaccgata tattttttta tgcgttttat tcgacctcct gtttgggtat
    40561 agagtcctgt atcaactacc gtaacccgcc aaggttcagc aacattatta tgtataatca
    40621 tttcattacc attagaaaag tcaaaagtaa tatcagacct atgcaaatag taatcggcaa
    40681 aatattcttt tataacataa cctttgtacc cgcaacaaat aataaagtca ttaaatccaa
    40741 agcatgaata gtatttcata atatgccata gtatcggagc atctccaatt tctatcattg
    40801 gcttaggttt taaatggctt tcttcactta ttcttgtacc aaatccacct gctaatatta
    40861 caatcttcat tattttctcc ttttaagtct aataaccacc catcatgtcc ataagccatg
    40921 agctttgtgc atttaatttg ttcagacttt gctccagtgc tgaaaattga ttccaatacc
    40981 tttgtctttc tctttcgaga ctcttcttta actctttcat tttttcatca taatctttaa
    41041 tttttaatga aatgttattt tggtcggatg aacgaccggt gtctaagcct gcttcttcga
    41101 ttaactttcc ggatgtaccc atagctccaa tattttctct aagtatatca agaatttgat
    41161 ttgcaatgcc ggactttgca tattcatccg tttcatcggc tatgcctgta aataagcttg
    41221 ctatttgatc gggagcttct attaactttt ctctgaactt ttcttcatta aaaaccaatt
    41281 taccgctggt atccattgat gctgctgaaa ttccgatatt ggacaaagcc aagtcgctta
    41341 cggaggtcat accgctcatg cttgctctca ttttgttcaa taggcttgta atattcttat
    41401 cgccgaagag tactcccttt ttagcttcct ttaaccaatt ttctatttct ttttctttca
    41461 tttcatcctg ctgctcggga gttaatggag gataatctct tcttggtttt tcttttgttt
    41521 tagtgccgag taaatctatt atttcattat aatcatcaat aaactgcttc actctttcaa
    41581 tcacttcatc agtgttattt tttacatcaa aagttatcgg gttttcttca ggtgaaagta
    41641 ttgcattttt gcctaattca acgtttattc catcaattga gaaattagca gtgcttcgtg
    41701 taatatctat atcttgcccg tgaatggtta cattcatcac tgtgtcttcg cctcttattt
    41761 ctatgtcact gccaatactt cctccgaaaa gcttatctgc caatgctccc gtaatttcaa
    41821 tttttgatgc tttaccggta tctttagaca cgataacaaa tttatctgtg gtattggaat
    41881 atgtaatatc aagatcagat tctttgttta tctttttaag cacttcggaa attgacatat
    41941 ccttactaat tccatctatt gattttccgt taataataat agaacaatct gttacttctc
    42001 catatatatc acctatagtt ttcgaaactt ttaatctgtt gctgtcccct gatttaattc
    42061 ctgttagtat gcttaaatca ttgctgatat tgcttacact aaaaacatcg gtacttccgg
    42121 aagcagaaaa cattattgaa gtttgggaag catcaagttc tgcatttacc cttccggctc
    42181 cataaacttc agcaagtttt gtattcagaa aatctgctaa tccttcagca tcataagtat
    42241 aaggtgaacc gtcaggctta ccgtcaacca tttcagaaag agctatagcc ttcttcactc
    42301 cgttaaaatc aaatgtaatg ctgctgctta ataaaacatc ttccgatatg ttaccgatta
    42361 tttcatcact tgaaaagctt tgagatgaaa cagaatttgt tgtatatatt gcagctttag
    42421 caaccgatgt tatactattg attttaaaat tgcttataac atctgcactg ccggtcacgt
    42481 ttacgtaatt agatgaggat ttatatgtat atgattcaaa aaacttagga cttagaatat
    42541 tagtctttga gccggatgaa taagagaaat atttattgtt aaaggctaga agctttgaac
    42601 ttatttctct gtatgcttcc tgtttgtaca acaatttttg ttttgcttga tattgcttat
    42661 ttattttatt cctggtggcg gctgtcattt gattgactaa atcatctgta tccaatcctg
    42721 acataaggcc gcccattcct ttgtttacag atgagccgta taaattagtt gaaagtattg
    42781 acatatcttt ctccttttct tctatattat aatttccttg tcttcatatc tatctattca
    42841 tatatcgtca ataattccaa taaattaaac aaattatgaa ataaattaaa atacaaccat
    42901 agctttatgg ttgtatttta tattgtacag aaagtaagct tatagagtga attgttttac
    42961 taaatcgttg agaatcaata cttgtcctga taattcttcg ctgttggctg cacttgcctc
    43021 tgctgttgcc gcattctcct gaactaccga tgatatttgc tcaatgcctc ttcttatttg
    43081 atttactgaa atagtctgct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43201 ngctcttgca gcttctacag ctgcatttaa tgcaagtatg ttagtttgga atgctatatc
    43261 atctataact tttattatct ttcctatttc gcttgatgaa ttgcttattt catccattgc
    43321 tgatagcatt tcgcgcattt gcatgtttcc atgctcaact tccttggaag cttgcattgc
    43381 cttgctccct gcattttctg catactctgc attgtttacg atagttgtgt aaatttcgtt
    43441 gatttcagct gataattcct ctattgcact tgcctgctcc gttgttcctt gagccaatgt
    43501 ttgtgctccc gttgaaacct gctcggcatt catgttgatt gcaattgagg attgttgtat
    43561 tttccctatt gcatctttta aggtattgga catatttaac aaagattttt ttaatttggc
    43621 gaattcacct gcataatcat ttctaagctc catgttcaaa tgtccttcag ccattttatc
    43681 aataactccg acgctttcat caatataaat aatatataat tttaaccgct ctgtcagttt
    43741 tcttatagaa tttgcaagta caccgatttc atcgtcgctg tttatatcaa tatttacatc
    43801 taattcgcct tcggctaatt tgttggtaat acttgttata tccgttatcg gttttgtcaa
    43861 tttatttatt aatacgtata aaacaccaat cagcacaacc aataatatta cgaatacagc
    43921 tataataatt aataccaact gatttgtatt caacatgaat tcttctctcg gaagcgatga
    43981 cataatctgc caccccgtat ctccgatttc cacactgttt ccgatgaacc tgtcataatt
    44041 atatgtgaat tctactacgg acctgtcttt attgataaca gattgaacga tattatcgct
    44101 taagtttatt tcatcaactt tttttaataa gttatcctta tctttatggg caattataac
    44161 attatttttt gataacaatg tgaaatatcc tgtgtctccc agctcatatt catcaaccat
    44221 gtttattaat ttatcaatgg ctatatccac tgccgtcaag cctacaattt caccgtctac
    44281 attaaccggt gcagatatgg ttataactat tcctcctgta atagcatcaa tataagggtc
    44341 ggtaatgcat actttatttt ctgtaactgc attgtaatat tctttggaag taacatcata
    44401 ttcggattgt gtatagatgg ttggagatgc caaataataa tcatcttctg caatataagc
    44461 agtaagaatt atttctgaat ctgctttttg aatatcatca gttgttgccg tagctatgtc
    44521 gaaccctctt gttacaggta atgtttggta tccttctgca ttaatcataa attcttgtaa
    44581 gttcttgtca tgagccattt gctctacaac ggtaatatat ttttcaaaaa aaacttctgc
    44641 cttattgctt atgcttaacg ccgtttcatt cagtatttgt cttttgttat ccttaaacct
    44701 tgtactgact gttttaacaa taattattcc tgttattagc agtgccgaaa aacatacaat
    44761 taaaatagta gttgttatct ttttggaaat actcttattc gttttcataa tccctccaca
    44821 tgatattgat atggttgtcc aatgtttata tcttaatatt tacaactatg tttgtttctg
    44881 agtaatttaa cttttcctta actgatttta gctcggtatt tatattttta cgaattactt
    44941 tatcgatttc ataaatttcc gtaaaaaccc tgctgatatc tttgtttaat tttttaattt
    45001 catttgtttc aacatagttt tctttgcttt ttacttcgct tattttttcg ttaattacat
    45061 ttattttatc tatatactgc tgcctttcat caagcaatga attaacccgg ttataatcga
    45121 ttattaagga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtgatatctg
    45241 atacttgcca tccaatgtag ccgttaaata ataaaatata tgtctgactt ttgttaagaa
    45301 aatgtttgca ttagcatagt cttcatgctc cattaataat ttgctttgat ttagtttttt
    45361 aattgcttca tcgaataaaa gaatcagaag ctcacctttt gtcattgtat ttactgaggt
    45421 ttttttgtat tgctccaatg aatttaacat aatttctcct ttttactcaa taatctttgc
    45481 tttatcttcg tgctacttat attatcgtca gaaattttat attgttaaat gttttgttaa
    45541 aatttcaaaa atatcttcta acttggcctg caccgtaaca gctccaatat tgaatgcagc
    45601 catcggcatt ccgtaaacgg cagaagtact ttcatcttgt ccgatagtat acgctcccgc
    45661 ttttttcata tttaaaagac cttcagctcc gtctttgccc attccggtaa gaataattcc
    45721 tactgattta ttaccggcaa ttttggccgc cgaattaaaa agcaaatcta ttgaagggca
    45781 atatccgttt attttgtcgc cttttttgca ggatacatag taacctttat cgtctttttt
    45841 aagcaccata tgcttgtccc cgggagcaat taatacaatc ccttgtttta tcctgtcacc
    45901 gttaagggct tctcttactt ccattttgca tatcttatta agcctgtccg catacatttt
    45961 tgtaaatccg ataggcatgt gctgagctat aagaatcgga ggaaaatcct ccggcaattc
    46021 tttaagtatt ttgataattg cctcggtacc tcccgttgaa gcccctatta cgattatttt
    46081 attaattagc atattattcc tttttgtata tggccggttt aatatagaga tattttgact
    46141 gctctctttg aattatttcc gcatgtccaa tcatcaaata tccccctgac ctggttgcat
    46201 tgtataattt gtttataatt ttgttttttg tttctaaatc gaaataaatc attacatttc
    46261 tcataaatat tacgtgatac ctgttttttt ccaatatcgg agccattaaa ttagatgctt
    46321 taaatctaac ataattcttg atttcgggta ccacctggta tttattatcg tcgagcttta
    46381 tgaaatatct ttttataaat gaatcgggaa gtttacagat gctgtctttg gaatatatnn
    46441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tcgttgtttt ttacacttct
    46561 caaatactgg taaaaactgt tcattccctt tttttcaatt aaattagata gtctcccctc
    46621 aattaatatc tttttctttg aaaggtctat cccatagttt ttatttatgt attttacata
    46681 ttccaaaaat tcgttatcag ttattctcac catatctatt actttccatt aaatttccta
    46741 catctaatat taaattaata cttccgtcac ctaatattga gcagccgtaa attcctgatt
    46801 ccttaaggtt gtacttgttt aatattattg gaaggggctt aacgacaact tgctgctccc
    46861 ctattatttt atcaacaaac aagcaggcac tgttgtcacc tgattccaca aaaattaata
    46921 taccgtcttc gatatttgta atttctgtat taatgttgaa atattcatgt attcttatta
    46981 ttctgtagca tttttccctt atcaatatca tttcgccttg ctcgggatta tgaataattt
    47041 cacttttttt ggttttaaat gattgatgaa tactcttaat aggtattata aattcagagt
    47101 ttcccactac aacctgcatg cctgatacta tggttaaagt aagaggaatt ttaaatgtaa
    47161 ttttactccc taatccttca ttgctctcta atacaattgt ccctcccacc ttttccacat
    47221 ttgatttaac aacatccatt ccgacacccc tgcctgaata ctcggttata tcatcattgg
    47281 tggaaaatcc gggaagcatc aaaaattgga atgcttcttt ttgtgtatat tcgcttgctg
    47341 gtttggttaa taaacctttg gcttccgcct tttccaatat tttttccgaa tcaaagcctc
    47401 ttccgtcatc ttctacggta atgtgtattt ctccccctgt attaaaggca gataaggtta
    47461 tttttccttt tttgttcttt cctttactta tccttttttc ttccggttct ataccgtggt
    47521 ccattgcatt cctgacaagg tgcataatgg gatctgaaat acaatctgct attgttttgt
    47581 cgatttcagt atcctcaccg tttaatacaa gctcaacatc cttgtttaat tcctttccca
    47641 tatctcttac taccctgtgc attttattaa atgtgcccga aattggaacc atcctcaagg
    47701 acattacaat atcctgcagc tcatcggtaa gttttctcac ttgtcttgtt gattttttaa
    47761 agctttcaga atttaatctt tttatatctg catccgactc aaccattgat tccgcaataa
    47821 caagctcccc aacaatatct acaagcttgt caagctttga aagattaact gttattaagc
    47881 tttgcctgca ttgttttgaa tttgttgatg aaattgaatt gtttaaatca gtggagcatc
    47941 ttgtttggct gataattgtt tcatcttctt tattattttg tacttcctca ttgtattcaa
    48001 atacagtata atttttgacg tttaatgctt tactgattac ttctgttgcc gtatccagat
    48061 attccttatt cacatatacg aaaaagccgt tttcaattat ttcttgcgat gtttcgggaa
    48121 tgttttcgat attttcaggt ttatattcaa attcaatacc tgtttccctc aattgattga
    48181 ctataataaa tgctcttaaa ttttccatgc ttacatcgtc atcaaatatt attcttatgg
    48241 cgtatgtgca tccctttgtt tggttatttt cttcagtaat tgcaatattt gattttagat
    48301 tttctatttg gtatatttct tctttggttt cttttttgat tgagtttaaa aataacttta
    48361 tttcattttc taaagggtct aaatttttct caagctcaag cccttcttct accttgacaa
    48421 tttctccttt tataaaatca gtaattttaa aaactaaatt tacaagttct tcgttatact
    48481 tttggtctat tccattttct ctgatataat aaaacaaatc ttcaattttg tgagaaatag
    48541 tcattatact gtcaaattgc atcatggcgg atgagccttt tatggtatgc attgccctga
    48601 atatttcgtc catgcattct ttatcaaaac ttaaattgct ttcagctttt aacaaaatat
    48661 tatctaatag ttccaataat gaatttgttt caaaaagata catatcaaga acagaatcta
    48721 aattattatc catattactc tccttgttgg tttcctatct ttatatcgac ctttattatg
    48781 aattgttaag ctattataac gaaaaacttt ttataaattt ttcgataata ctcttattta
    48841 atttattatc gtgccgatat acaagatgag gggggacaca cctttaccat atggttagtc
    48901 tttggagcat aaggaatgtc ccccaattgg tattaacttt ataaaacagg agattatttt
    48961 gaataatata agtgtcaaga ctaataatca tattagcgaa aatcccatta ataagcctaa
    49021 aattaaaaac gaagtgcctt ttgatatagc tgatcctact aaggttacaa agacgcctga
    49081 gaagggttat gaaaatcaaa tcagacaaga acttttcaat tacaatcctg attctgtttt
    49141 tgacaagttt attaaatcat tgcaaaattc tcctgtatta tatgaagaag ctaagaagct
    49201 ccttttaagc aaacagttta tcaacaacaa tattaaaaac gacccggtgt taagtgtatt
    49261 ttttgattct tttcttggta atattaaaat gaatgaagct gaaatattgg actttcttaa
    49321 gttccagcaa ggaacataca ctaaatttca aggcgaattt ttcgatttac tgagaacttt
    49381 gttaggcaat aatcctgaca acaaaggact tcaaacggtt ttaaaaaatt tcttaagaag
    49441 ctatgactgc tttgtatcag tgaaagaaat atacggctca attaacacaa cctttaaaaa
    49501 tatcgaaaaa aacattccgg aaatgttaaa agcttctttt aatgagctaa cggaaaaatt
    49561 tatttacgat aatacaaatg ccatggactc aaacttggag ctgttgaaaa atgaaattct
    49621 gcccttcatt ggaagatata tttccaaaat gaatgatttt ggtattataa gggactatgt
    49681 atccgtattg gttcacaacc tggtaagatt ggaatatggc tcaaaaacta atttttctga
    49741 tgaattggaa aatttgtttg aattcttaaa atataatttt caacttgacg gaaaacagat
    49801 ggaattatta aaaacaactt taatcaatac ttatgaaaca aaatcaaata ttaaaaacga
    49861 ttctattgaa tcctttttca aactccttga taaagggata agcgaaagtg agaatgctgt
    49921 aactaaaggt ttaatggggg atatgaccga gtcgatttta tttagtcaaa atgttaatat
    49981 tcccttactc cacatgtttt taccgttaaa ttacaagggt atgtttatgt tttccgaaat
    50041 atggataggc aaggatgaag acagccctga taataaaaga aacctggaaa aacaaaaatc
    50101 ctataaagtt tttataactt ttgatatcca aaacttagga tattttgaaa caactttaaa
    50161 attaagggac tccgtcttgt cgcttgatat ttatataccg ggcagtttat cggaacagac
    50221 cgagaaaatt aaaagtgatt taaactttct cttaagcaaa aacaatgtta caatagaaag
    50281 cataagagtg caggaatgca ttaaagccag gcggtttagc gaagttttta caaatctgac
    50341 tgaaaggaaa aatggtgtag atgtcacaat ataataataa atcaaatcat gctgttgccc
    50401 ttaaatataa tgcggctaaa aacaacgctc ctgtcgtaat agcttcaggt tcaggttata
    50461 ttgcagacaa agttgttgaa atagccgaaa aaaacggagt gcctgtctat agagatgact
    50521 ccttgtcagc catattgtcc cagcttaaaa taggcagcga aattcccgaa gaattattca
    50581 gctcaattgt agatatttat gtttatttct tgaattttaa aatgcctgag agtgaggctt
    50641 aaaaataaca gcttgggagt aatcatgatt gatactgata aaatagtaaa aatatatgat
    50701 gaagatagta aactgattgg aaaaggtcaa ctgttaagct taaatgaaaa aataataaag
    50761 gttaaaggta ccggcttgcc gattttaagc cggaaaacaa atgttattat tgaaatttac
    50821 agtgaatttg taggaatttc ccgttactgc tgccagattg atttggcatc cgataatcag
    50881 cttaatgctc atatagttaa aaagaagccc gatattgaaa gaagaaactc attaaaggtt
    50941 cggaccgact tatcctatta tatagaaagt ctttacagaa atgataaaga cataacaaaa
    51001 gactttccaa atatgaaaat taatttgatt aacttaagca ttggcggcat gttgatttca
    51061 tcaaactacg aattatacat aaatgatatc atagcttttt attttagatg tgaaaaaaca
    51121 gctgtattgt tgaaggctaa ggttatgaga attgataaaa tatatgacga taatcaaaag
    51181 tatttatctt tctacaatta cggatgtatt tttgaagaga tgcctcctca tatagaaaag
    51241 attattactc ggtatttgta tataaggcag cttgaaatgc ataaagacaa gtgggagaaa
    51301 ttatgaaaaa gattacaatt atatttatcg ctcttttaat cataagcagt atgaatatat
//
LOCUS       DAOPUE010000004        45089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_766_length_45089_cov_9.702758, whole genome shotgun sequence.
ACCESSION   DAOPUE010000004 DAOPUE010000000
VERSION     DAOPUE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 45089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45089
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_45089_cov_9.702758"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..2984)
                     /locus_tag="PKH42_00820"
     CDS             complement(153..2984)
                     /locus_tag="PKH42_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA18997.1"
                     /translation="MTRKITVWFLIVSMIIMNATFVFAAEPELIITGTGVEEEVVISS
                     GDWSKYKMVERIYSTNNSLGFHKIIKAKGYDLFELMGNGLKTDKDYSVKFTCSDGFEF
                     TKTISELKSAYYFSDFTLESKQAVMPMVAKYTTVMADFPKNIFAPPLTWTDIPITEKD
                     LDKDFPKLVFGQTDIDDMNMSKWGKEVIKITVGDEIVKVTPSSPYKHISYEGEPYNVD
                     AVTGATFTIEGPAVEGYRAISLRQIEEDTTGQTIDTYYEKVNGKVVEVSYEGINVKYL
                     IDNYVKAKSNAGNIIFKDKSRKTIITADISEAGNYMVAYGINEVPLVYLDTDEGYIKE
                     KHNDNGCFKLVYEQDKNKAEEFSNVAYIYIEEKDAKNIFEHSYAPYNAEKYTDYELII
                     HGSALGKEVRYKVKDIEAMTDLHETHEYSLSNSEYFWYYNTYKGVPLWDLLLKAGLDP
                     DIDENTSVRFIAADNYNFAPMTIKEIKDRSLYGYYEKSAMDKGDGTFPGEYVDPLYTN
                     LPVMVAYGFNKYPYVIRPTDEGYNPGLGNDGGPLRIIFGKTNYNDTNGSNQVQFAKEI
                     IIGEGESLATDTQGIGEGVSTEIEIDESWNHNQGVYKEYLDMPVLRVTGSQVKQPMTF
                     TLRQIEAMTEYAVRDTYTGDGIREMQGIVLWDLINNVVGLKEDVEVPSIRVFSGQNYN
                     QILRSNEQVVNGVLNSQGQTKKIILAYAADGYPLVPDESSIGYVNNNAYGPLRLIIEE
                     SKSMWVKWTDCIVVGTGDYEAPDMKDVKIDTFSDIESHWAKDSIIEMAAMGYAKGSDG
                     LFRPNDNISRAEFVSLLIRVLGMGNEDAIPKFNDVKATDWFAKDVAIAAEKGYVKGYE
                     DGTFKPNSPISRQEIGTIIGSLLEDDITNAHEILSIFSDEVPEWAKVPVAKTVKAEII
                     KGLPGNIFGGAQNTTRAEAAVMLLRYLDK"
     gene            complement(3036..4190)
                     /locus_tag="PKH42_00825"
     CDS             complement(3036..4190)
                     /locus_tag="PKH42_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA18998.1"
                     /translation="MKKFKVIAMVLAIVMILSQSLVFAEDYAVEIGGSLAATELKLTL
                     EDLKSMPEEAQIAQEYVYNSKGGEKTVQVKGVSLAYLLKEMAGVTAEEGNVEFFAADE
                     WPIDPQPLQDVLNDELKFVLAYEVDGEAINNDEGIIEEITVYRNLKYEDEFDTVYKLI
                     NKITVGEAEAVEEEEAPEEETIEPIEEETEETEETEEVTFTDITEEYKFAEAAIYDLA
                     KRGIIDGMGGGLYAPGNEFTREQFCKIIVVALEYELKEYEGAFSDIDDDRWSAPYVQA
                     AVDSGLFVGYPDGTFLPEKVITRQEMALVAARAAVAKGLVEQAKVDKFVMEKSDYIDK
                     EDVADWAGNAVAWLEAQDVFAGIAVEKFEPARNVNRAEAALVVFNTLFSE"
     regulatory      complement(4291..4407)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(4461..>4610)
                     /locus_tag="PKH42_00830"
     CDS             complement(4461..>4610)
                     /locus_tag="PKH42_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOA18999.1"
                     /translation="GTSLVVYPAAGLINYFKGSKLVLINKSSTPMDRRADLVINDSIG
                     KVFED"
     assembly_gap    4611..4710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4711..4934)
                     /locus_tag="PKH42_00835"
     CDS             complement(<4711..4934)
                     /locus_tag="PKH42_00835"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOA19000.1"
                     /translation="MIAELKEIISKSDNIVFFGGAGVSTESGIPDFRSVDGLYSKKYK
                     YPPEMMVSHSFFRSNTEEFFDFYINKMIFL"
     gene            complement(5110..6198)
                     /locus_tag="PKH42_00840"
     CDS             complement(5110..6198)
                     /locus_tag="PKH42_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOA19001.1"
                     /translation="MKKIIILIALIMMTGCGAPTAEIPENPPAVEAPGEEYPEEDKED
                     TDNLNEDKELSDEEILASIDLSLNPNELGEIMILMYHGIGPEESVWQRTPDNFRKDLE
                     YMYQNGYRMISLNDYAKGEIKTEAGYTPIILTFDDGRQNNFSYIEENGKIVIDPDCAI
                     GILEEFKNKYPDFNVTASFFLGTNLFGQKEYAEKKLNWLIENGYDLGNHTYSHNEMAE
                     LNSDEIIIEIGTVNNIIKQYLPDYAVETLALPHGSNPKDEFIESVLEGEYEGNKYRTL
                     AVLDVGWRPAYSPFDILTDFSNLYRVTASEINVDNCGINDYFKQYEENKRERFISDGN
                     PDVVTIPKRHVDYLNMDIVGDRIVNIYE"
     gene            6434..6955
                     /locus_tag="PKH42_00845"
     CDS             6434..6955
                     /locus_tag="PKH42_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOA19002.1"
                     /translation="MISSNKSTQDKLKLLYILDYIDIPLTNNEITNYILEYSMMDYFT
                     LQLLLSDLCEVKFIHLKPYNGSEYYSVSKSGKAALEMFGDKLPEYFINEVAKNYSGIK
                     KHIEKQREIFGHYYKRKDDEYIVTLQVSENSTTIFNLSINVTDEITAKKIIKKWENNP
                     DKIFGEIINALTN"
     gene            complement(6952..7842)
                     /locus_tag="PKH42_00850"
     CDS             complement(6952..7842)
                     /locus_tag="PKH42_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19003.1"
                     /translation="MYDEKIVTTGICQGKLFLFIMELLKKDYSFKKALYLGAGDSFAI
                     HSIEVFNDAVYMTDSFNNKIYKFDSVNNFFETNVGRDPRHMCTDKDNIYVANFESDNI
                     SIIELDSFTLTGSLPAGIKAHDVIYSEKNKSLYTTCYEENEVLEYSIDNGIRRRFKTD
                     GKPMHIFVLDDTIIVMTYYVNGIVQTKINFINLSNKEIEDTIDIDGLASDFLLDYENN
                     MLYIINIVDKSLYIADSLLRKIIKKIYLGGYPESISCGSKNIYVTNSKKQQIDIIEKQ
                     GHNISTLKLMFTPGLIKIIP"
     gene            complement(7965..8672)
                     /locus_tag="PKH42_00855"
     CDS             complement(7965..8672)
                     /locus_tag="PKH42_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA19004.1"
                     /translation="MIYRQNQQLCPNIYSMPYEVRPGDTLNSIAARFGTNLVGLIELN
                     PTLRLRSLTEGMTICVPRVPERPPCVNGAYYTIREGDSFYNIAQRYGIPLNLLLEANP
                     EANPYDLKVGQEICIPDVPPGCPFGTVVTIQEGTRLSDILISYNLSLNELRDANVNFN
                     PNIIVPGTELCIPQETFMQCPEGTTTEYVIQAGDSLSTVAIANNITPSELLIANPHLR
                     PANFLIVGTRVCIPTAR"
     gene            complement(8747..9190)
                     /gene="rnhA"
                     /locus_tag="PKH42_00860"
     CDS             complement(8747..9190)
                     /gene="rnhA"
                     /locus_tag="PKH42_00860"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOA19005.1"
                     /translation="MKKVNIYTDGACSKNPGPGGYGVILEYKGHEVELSGGSENTTNN
                     QMELMGVITGLEALKEPCDVTIVTDSKYIADSFNKGWIDAWQKKSWKKSDGKAVLNKE
                     LWQRLLKAMEGHKVKFVHVPGHKGHSYNERCDKLAVEERKKFETK"
     gene            complement(9187..10368)
                     /locus_tag="PKH42_00865"
     CDS             complement(9187..10368)
                     /locus_tag="PKH42_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HOA19006.1"
                     /translation="MFKVALFCDIPQETLNLLPEDKYYLIREETSEADGIIIRSLDIH
                     DMKFSKQLKAIARSGAGVNNIPVERCSEEGIVVFNAPGANANAVCELTFLGLLMSSRK
                     VIDSIDWTKKLESNVVETVEREKSKFVGPELKGKTLGVIGLGNVGHLVANLGTKLGME
                     VIGYDPYISVKNALSLSWDVQYTGDINDLYKRSDYITIHVPYNNETKDYINKDNIKLM
                     KDGVKILNFARHGIINEDDLIEALESGKVSYHVSDLPNEKLLKAKNTLCIPHLGGSTI
                     EAEENCAEMAVTELTEYLEKGNITNSVNFPDCAMDRYPGTDRIVVLNKNIPNMIGKMT
                     TALASQNVNIIGMVNKSKGDYACNIIDVIGELNEDMLNGLVEHYGILRVMLIKNKRSK
                     K"
     gene            complement(10473..10613)
                     /locus_tag="PKH42_00870"
     CDS             complement(10473..10613)
                     /locus_tag="PKH42_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19007.1"
                     /translation="MSDEVRATNFRCCDDRSNDWFEWLIILGIIFFLLGGNNFFGRGF
                     GR"
     gene            complement(10850..12010)
                     /gene="thiI"
                     /locus_tag="PKH42_00875"
     CDS             complement(10850..12010)
                     /gene="thiI"
                     /locus_tag="PKH42_00875"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOA19008.1"
                     /translation="MYNIAAVSFGELFLKGKNRGKFEQKLIEQIKYALKEFNHVSVYK
                     DSGKVYVETKNEEDMDKIVEKVRKIFGISNISPSVKTSKDVDIIIEKTIELFRYLLSK
                     EDIKTFKIKTKRSDKEFPLKSMDFSALVGGKILENFSFVKVDVHTPDVEVYIDIKKCC
                     YISSERIKTLGGLPLGSNGRALLLLSGGIDSPVAGYMIAKRGVEIDALYFHTYPFTSE
                     RANEKVIKLKQKLEEYCGKIKLYSINMLEIHKAIREFCKEEETTILARRFMMRIAEKI
                     ALENKIDMLITGESLGQVASQTMKSMAVIEDAINMPILKPLVGLDKSEIIEIAREIGT
                     YETSILPYDDCCSVFAPQHPLINPKLESIKRSESNLNVEELIEKVLLTLEIL"
     gene            complement(12012..13154)
                     /locus_tag="PKH42_00880"
     CDS             complement(12012..13154)
                     /locus_tag="PKH42_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOA19009.1"
                     /translation="MLVYLDNSATTKVRDEVLKEIIDAHENYYGNPSSLHRMGHKVEK
                     KIDEARTLSAKLIGAKPEEIYFTGGGTESNNIAIFGHLSNLKEYNNVITTNIEHPSVY
                     NVFKQLEDRCLVKYLETDENGKINIDELHNMVDENTLLISIMHVNNEIGAVQELNEIT
                     AIIKRKNKNAKLHVDAVQSYGKIKIDVNKTPLDTLSFSSHKIHGPKGVGGLFIRSGIK
                     ITPLIYGGGQERGIRSGTENTTGIIGFGKACKLMYDNFDEEVNKLYGLKNTYIKRLEE
                     IEDIKINSPIDGAPHIISVSFKNVPGEVLVHYLEDKNIFVSTGSACSSKSKDSRILEA
                     IKLSKEYRAGTIRISLGHFNNFEEVDYVIDNIKKSVKDIRSITKRG"
     gene            complement(13427..14236)
                     /locus_tag="PKH42_00885"
     CDS             complement(13427..14236)
                     /locus_tag="PKH42_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOA19010.1"
                     /translation="MNILSDKNVMNNQIITKEETELLINKTKIYEVVRVIDGKPIFLK
                     EHFQRMSESINLSGIEGNINYEEFKNSVELLIKENSFKNLNIRVSYFYDKKPLTLFYF
                     IESYYPSKDEFAEGVHTVTVKMERANPNVKSFQKEYKDKVTRIIKENNAFEAILINHD
                     NTVSEGSKSNIFFVEGNKLITSPDSSVLLGVTRSKVISVCEENSIEVHKRIIRLDEIE
                     SFDGVFLTGTSNDVLPIKTVDNIVFNSSENEVVKKVSRLYLGEVKKEIEKG"
     gene            complement(14250..16550)
                     /locus_tag="PKH42_00890"
     CDS             complement(14250..16550)
                     /locus_tag="PKH42_00890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOA19011.1"
                     /translation="MDNIKLSVRELVEFIYKSGDINVRNLGADRAIEGIKAHKILQNQ
                     MGENYYKEYYLKGEFVLNDIIFLIEGRADGILIEDDETIIDEIKSTYTDLSLINGEYS
                     TAHMAQAKCYGYMYGLSNNLENISVQLRYYNLDTKEVKTIKQTFSTEELKIFFYSLLN
                     TYIDWAETIINLRKERNRTIKELNFPFEKYRKGQRDFSIAVYLSIRSGKKLFAQAPTG
                     VGKTVSVLFPAIKSLYEKNAKIFYLTAKSSTKTIAFNTVKMMHKQGLRLRTTVITAKD
                     KICFMDETKCEPEYCPYAKGYYDKLNIPLRETIKNNCMYDREFIEELGKKYELCPFEL
                     SLDLSYMSDIVICDYNYYFDPRVALKREDLFNKDKDILLIDEAHNLEDRVRNMYSPEL
                     IKEEFYEVYKIIKSEKSLSKELFNINKKFINIKKSIDEAQILKETPEELINALRKFIA
                     KADKYFNERKNEKIPEELTDIYFKSIFFIKISEIADDNFCYYADSANNKTMVKLFLID
                     TSQILKEIERNARSSIFFSATLTPLKYFRYILGGEEDDYILKLSSPYDKENLNLMITS
                     DISMKYTKRDSNIEKTCEYINTLITEKKGNYMIFFPSYNFMNKVYSVYESLYDTMNII
                     VQSQGLNEEEQMDILSRFENERNVVLFTVVGGTFSEGIDLPLDKLIGAVIIGTGIPQI
                     SFERNIIKAFFDEKMNTGYDYAYKYPGFNKVLQSAGRVIRTEDDRGTVLLIDSRFCQY
                     TYLKLFPDHWKHFKKIKTSEDIKNLL"
     gene            complement(16649..17035)
                     /locus_tag="PKH42_00895"
     CDS             complement(16649..17035)
                     /locus_tag="PKH42_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19012.1"
                     /translation="MHYVVERKVNSDIKCGKKIIEEILGSIEGLIDPNTYFNTKLILN
                     ELMINSVVHGNCGDLNKMIYIKVYINESRIIVEVSDEGSGICYEHKNCGEYDYLECGR
                     GLMLVEGLSDKLSIEGNTVTCVQYLK"
     gene            complement(17134..17727)
                     /gene="yihA"
                     /locus_tag="PKH42_00900"
     CDS             complement(17134..17727)
                     /gene="yihA"
                     /locus_tag="PKH42_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOA19013.1"
                     /translation="MIIRKAEFLISAVKKEQYPNTGVPEIAFAGKSNVGKSSMINALV
                     NRRNLAKTSSQPGKTQTLNFYNINDEFNFVDLPGYGYAKVSKAEQAKWADMIDLYLHS
                     RQQLKEVILLVDIRHEPGRNDLLMYEWIKSFGFKGYIIATKADKLSKSQQIKSLNNIK
                     KTLQIKDIGLIYQFSSTSKLGVEEIWILFESILNLHD"
     gene            complement(17778..20105)
                     /gene="lon"
                     /locus_tag="PKH42_00905"
     CDS             complement(17778..20105)
                     /gene="lon"
                     /locus_tag="PKH42_00905"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOA19014.1"
                     /translation="MIKNYKIERRNLPLIPIRGIGIFPNTVIHFDIGREKSINALEEA
                     MLEDSDIFLTVQKVADMESPKEDDFYEVGVICKIKQMIKMPGDNIRVLVEGINRAKIV
                     SVTQDDPYFEVALDEYIYDDSLPIDDNLEAMVRLTLNNFEEYALIYAKIAPDAVISLK
                     EIKNPDKLADVVASYIYLKTNQKQALLEIFDPYKRLESINKMLAKEIKVLQIENEINE
                     LVKKQVSEFQKDYYLKEQMRAIQKELGEDEGTDYEVEEYLQKISKANMPKDVKEKVEK
                     EINRLLKISPSSPDAGVIRTYVDWLLDLPWNKKTKDNTDLSRARKILAEDHYGLEEVK
                     ERILEYLAVKNIKKDMKGPILCFVGPPGVGKTSIAKSIARAMDRKYVRISLGGVRDEA
                     EIRGHRRTYIGAIPGRIISSIAKVKVNNPVFLLDEIDKLNSDFKGDPASALLEALDPE
                     QNNTFTDHYIEAPFSLQNVMFITTANTTSTIPGPLLDRMEVIEISGYTDIEKRNIAEK
                     YLIKKQLSEHGLKENQVKISTNAINEIIERYTRESGVRNLERNIGKLIRKAAVQIVEK
                     NKSTVSINNLNIKKYLGIPKYDYDIAYEEDQVGVVTGLAWTQVGGETLFVEVNTMKGD
                     GKLQLTGQLGDVMKESAMAALSYIKANAENFDIDNKIFKETDIHVHVPEGAIPKDGPS
                     AGITMATAIISALTKKPVRKDVAMTGEITITGRVLPIGGLKEKLLAAKRAGIKKVLLC
                     SKNKADVSKIDPKIIRSMEIVYADKIDDVVKEALR"
     gene            complement(20145..21404)
                     /gene="clpX"
                     /locus_tag="PKH42_00910"
     CDS             complement(20145..21404)
                     /gene="clpX"
                     /locus_tag="PKH42_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOA19015.1"
                     /translation="MSKVNEKHIKCSFCGKSQEQVRRLIAGPDVYICDECIELCYNIV
                     DEDIDLFEEYDFTELPKPKEMKAKLDEYVINQDNAKKALVVAVYNHYKRINYKTKDDG
                     VEIQKSNILLLGPTGSGKTYLAQTLAKLLNVPFAIADATSLTEAGYVGEDVENILLKL
                     LQAADFDVERAEKGIIYIDEVDKISRRSENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFIQIDTTNILFILGGAFDGIEKIIQRRVGKKSIGFGADISGNNSMDT
                     DELLKQVQSQDLLKYGMIPEFVGRIPVIVALEKLDKNALISILTEPKNSLVKQYKELF
                     KMDNVELEIEKDALEEIAKKALEINTGARGLRGILESTMTEVMYEIPSRSDIKKCVIT
                     KDTIINKSEPKLVLTKKPVSDKKENVS"
     gene            complement(21416..22000)
                     /gene="clpP"
                     /locus_tag="PKH42_00915"
     CDS             complement(21416..22000)
                     /gene="clpP"
                     /locus_tag="PKH42_00915"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOA19016.1"
                     /translation="MTLIPYVIEQTGRGERSYDIFSRLLKDRIVILSDEVNDATASLI
                     VAQMLFLESEDPDKDIQFYINSPGGSITAGMAIYDTMQLIKPDVSTICVGMAASMGAF
                     LLLSGAKGKRFALPNSEIMIHQPLGGMRGQASDIKIHADRIIKIRDTLNKIISEKTGQ
                     PIAKVQQDTDRDNFMTAEEAKIYGIIDDIIAKRK"
     gene            complement(22070..23452)
                     /gene="tig"
                     /locus_tag="PKH42_00920"
     CDS             complement(22070..23452)
                     /gene="tig"
                     /locus_tag="PKH42_00920"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOA19017.1"
                     /translation="MSSKLIKKEKNTVTLEFTVSQDEFDKAVNKAYLKVKNNINVQGF
                     RKGKAPRHIIEKKYGKSIFYDDALDIAVQEAYPKAVNELELDIIDSPKINVEKFEDGE
                     EIVFTAEVEVMPEVELPKYKGIEVVKTEIKVTDEDVERELKSIQEKNARIVEVTDRPV
                     QKGDFLTIDYAGFVGEEQFEGGTAENQTLEIGSNSFIPGFEDQLIGKSKGDEVTVNVT
                     FPDDYHAENLKGKDAVFNVKIHEIKAKELPEMDDEFAKDVSEFDTLEELIEDTRKVLE
                     KKAADQSKISNDNKVITKIVNDTKLEVPEVLIQREIEHMGRDYEQQFRQQGFTGKEYD
                     ELINNFVNQYKESARGQAEFNVKAELVLEEIIKKEEITVSDEELQEEINKIAESYKVE
                     EDRLEGFKESLMQSSKGYIEETLKKRKVFDMLVENAVFVDAPETVESIEAHETINESE
                     KAEEPEEDKE"
     gene            complement(23602..24168)
                     /locus_tag="PKH42_00925"
     CDS             complement(23602..24168)
                     /locus_tag="PKH42_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19018.1"
                     /translation="MKKLMLSGTVQIETRRFVLRKFQMSDAQDIYYNWLSDKDSAKYN
                     AWRVHTSIDETKQYLSEWTSLYKKNDYLHWAVVDKETKEVIGSVTVSNIKKWKKYCEV
                     GYTIAKKLWNQGIATEVLISILHYLTQEVGFEKVCAIHDIRNEASGRVMEKAGMKFVK
                     NKTHIFLNSDNIIMKCRLYEYRGMGTHL"
     gene            24413..25048
                     /locus_tag="PKH42_00930"
     CDS             24413..25048
                     /locus_tag="PKH42_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOA19019.1"
                     /translation="MPQEIYNASGIKIFGKRIKSLIYTTDLAIIKNNNADGVIAVYPF
                     TPQLAINQAIIDISPTPVFVGVGGGTTTGQRSIDIALNAELTGAYGVVLNAPTKNELI
                     SEMKERLDIPIIITIVSEKEDFESRIKAGVSIFNVSGGANTTNIVRQIREKHPYFPII
                     ATGGPTPESILETIRAGANIITYTPPPTGKIFSEMMEVYRHKITEELSTNK"
     gene            complement(25099..25542)
                     /locus_tag="PKH42_00935"
     CDS             complement(25099..25542)
                     /locus_tag="PKH42_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOA19020.1"
                     /translation="MDILNLLERIEDIIEDSSKFPLSNKVMIDKEEVLEVINEIRLKL
                     PDEINRASWVAKERQRILNEAQSEADELINKVKEQQKHLIEESEITRQAKIYANEIIE
                     EAERKANEMKVGAYNYSDEILSKLQDKIKEINNIIEDNREVLKKM"
     gene            complement(25545..26024)
                     /gene="coaD"
                     /locus_tag="PKH42_00940"
     CDS             complement(25545..26024)
                     /gene="coaD"
                     /locus_tag="PKH42_00940"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOA19021.1"
                     /translation="MKVLYTGSFDPITMGHLDIIKRISKKFEEVVVTVFNNKEKKYWF
                     SIEERCEMVREAVKDLENVEVDFTGGLVVDYCKRNNISIIVRGLRAVSDYEYELAISS
                     INKHLNNELETIFLVATSEYSFLSSSMVKDVAEHGGNIEGLVPDNVAQNIYRLLGRK"
     gene            complement(26021..26584)
                     /gene="rsmD"
                     /locus_tag="PKH42_00945"
     CDS             complement(26021..26584)
                     /gene="rsmD"
                     /locus_tag="PKH42_00945"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOA19022.1"
                     /translation="MRIISGTNKGKKLYAPEGKSVRPTGDKIKEAIFNIIGSIDEEST
                     VLELFAGSGSIGIEFLARGAKHCTFVDISRKSLNYVKKNLELCNFSDKAKIISSDYEK
                     AIINLSKDNEKFDYIFADPPYELNISNKILNKIVEYGILKSDGLLIIESDKSEKVIDN
                     EVANMIEYKEKIYGRTKISIIKYLEEQ"
     gene            complement(26609..28663)
                     /gene="recG"
                     /locus_tag="PKH42_00950"
     CDS             complement(26609..28663)
                     /gene="recG"
                     /locus_tag="PKH42_00950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOA19023.1"
                     /translation="MLDADIQYLKGVGPNRALKLHKLDIFTVSDLLNYFPVKYEDRRT
                     VKNINEICEQGKYLLKVRIFEKPKYSRIKKNMSIIKAICKDDTGFITLTFFNQDFLKD
                     KLIVGEYYYVFGNVKIAYGKLESTNPEIEFINKDWKAGKIMPVYGLTYGLSNNEITKL
                     VKTALETYYDEIQNVLPDEIIKKYGLISKKAAVKALHFPSDNKKYTLAKRTIAFEKLL
                     IMQLGLFTIKNNLTNELNEIVLRNTELSEKLLSSIPYKLTKAQLKVIEEIKEDLKSQK
                     TMNRLVQGDVGSGKTVVAVYAMLMAAGSGYQSSMMAPTEILALQHYDTIKEFIKMADL
                     DVKAEILTGSTKPKEKQEILDKLKGGEIDIIVGTHALIEENVEFKNIGLVVTDEQHRF
                     GVRQRARLSNKGKNPHILIMTATPIPRTLALMIYGDLDISLIDEMPPGRQKVITSIIS
                     EKEKTKAYDFIKSQVDSGRQAYIVAPLVDESENLDLNSAVEIYKELSTSHFSGYKLGL
                     LHGKMSNSEKEEIINKFYRGKINVLISTTVVEVGVNVPNAVVMLILNAERFGLAQLHQ
                     LRGRVGRGKCQSYCILVNESKSKKSKDRMNILKRTDNGFIIAEEDLKIRGPGDFFGVR
                     QHGLSKLEIQNIVNNVETVQEVQLLSKRILSENPYLKGSDYQNLKMEIMNLFKDETIV
                     FN"
     gene            complement(28707..30326)
                     /locus_tag="PKH42_00955"
     CDS             complement(28707..30326)
                     /locus_tag="PKH42_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOA19024.1"
                     /translation="MLIKYIDNVKFKKMLLGAAVNLENNKTIVDSLNVFPVPDGDTGT
                     NMNLTLQAAIKEINNLSESTITSLASAAANGSLMGARGNSGVILSQLLRGMSKALKGL
                     NHIDCKIIAESLKSASDTAYKAVMKPVEGTMLTVARETAEKAMATYTDYEDVIEFIES
                     LIQQAKDTLNRTPEMLNVLKQADVVDAGGKGLIYLMEGALAALKGVEIKQEITEQAVI
                     DTEVELDEFTSEDEIIFAYCTEFLIKNPIKTAEEFLTRIYDKGDSIVCAGNENVIKIH
                     IHTNNPGQILAMAVKFGELSNIKIDNMKEQFRERLKENHKNNQKKKKYGFVSIGVGKG
                     IEKVFTDLNVDVFLSGGQTMNPSTEDILEAANDINAEHIIILPNNSNIILAATQAKKI
                     SDKNLYVVPSKTIPQGIAALIAFKEDSDIDENIEAMSQAVKEVKTGLVTYAVRDTVFN
                     DLEIKKDQIIAIYEGDITNHGENPEDEAVELINKMVDEDSFLVTVFYGDNVDEKTAES
                     IKPRIEEIAEDCDIEIIYGGQPLYYYIISVE"
     gene            complement(30340..30699)
                     /locus_tag="PKH42_00960"
     CDS             complement(30340..30699)
                     /locus_tag="PKH42_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA19025.1"
                     /translation="MSVKIINENGSIYVDDQALAAIAGLAAMECYGVVGMASRNATQG
                     FFELLKREQLTKGIKVTSTEEDKINIDLYIVLQYGVKISVVAENVISRIKYSVETFSG
                     VSVENVNIFVQGVRVQK"
     gene            30991..31182
                     /gene="rpmB"
                     /locus_tag="PKH42_00965"
     CDS             30991..31182
                     /gene="rpmB"
                     /locus_tag="PKH42_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOA19026.1"
                     /translation="MAKFCEVCGKGTISGHSITHSDRKINRTWKPNVRTITVMEDGRA
                     LRKTVCTRCIRAGKIQRAN"
     gene            complement(31300..31497)
                     /locus_tag="PKH42_00970"
     CDS             complement(31300..31497)
                     /locus_tag="PKH42_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19027.1"
                     /translation="MNYFKHKKKCSPANKPSFFKFTEILGLMIATIGAIIIVQMLPVK
                     VWLFVLGILLIILGCTLFRLL"
     gene            complement(31599..32981)
                     /gene="asnS"
                     /locus_tag="PKH42_00975"
     CDS             complement(31599..32981)
                     /gene="asnS"
                     /locus_tag="PKH42_00975"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOA19028.1"
                     /translation="MKTTDIKDIFLSPENYEEVRIEGWIRTIRDSKNFGFIELNDGTF
                     LKNVQVVFENNLSNFEEIKKFSTGSAITVRGNIVLTPEAKQPFEIKATEIALEAPSDS
                     SYPLQKKRHSMEYLRTIAHLRPRTNTFSAVFRVRSLAAYAIHKFFTERGFIYAHPPII
                     TASDAEGAGEMFRVTTLDLDNIEKVDGKTDYSKDFFGRSANLTVSGQLEAEIFALAFK
                     KTYTFGPTFRAENSNTARHAAEFWMIEPEIAFADINDNMDLAEDMVKYVVSYVMENAK
                     EELEFFNNFVEKGLLEKLDNVVNSEFGRISYTEAIDILMKNNDNFEYKVKWGTDIQTE
                     HERYLSEKIFKKPVFAVNYPKEIKAFYMRMNEDNKTVAAMDLLVPGIGELIGGSQREE
                     RLEFLEKRMDEMGVPKDNMWWYLELRKYGGVKHSGFGLGFERLIMYLTGMSNIRDVIP
                     FPRTPKNADF"
     gene            complement(32993..33628)
                     /locus_tag="PKH42_00980"
     CDS             complement(32993..33628)
                     /locus_tag="PKH42_00980"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOA19029.1"
                     /translation="MKTALIVGNGDETAKRIIENIKYDYLICADGGLEKVSNYGIIPD
                     IIIGDFDSVASNVLKQYENRIPIEKFPSEKDFTDMELAVELAVSKGYKNIILTGASGS
                     RLDHTLGNILLMEKYFKDGVNISIIDNNNEMKIISDNSELFIEYKEGYFISIIPITDS
                     IQGLCLEGFKYNLDNVDVERGSTLCISNQIKDNKGKITLKKGRAIVIISKD"
     gene            complement(33625..34281)
                     /gene="rpe"
                     /locus_tag="PKH42_00985"
     CDS             complement(33625..34281)
                     /gene="rpe"
                     /locus_tag="PKH42_00985"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOA19030.1"
                     /translation="MKNKLAPSILSADFSNLGEAVKLVEEGGADYIHIDVMDGHYVPN
                     ITIGPAVVKDLRKTTSLIFDVHLMIENPDNFIEHFYKAGADIITVHQEATVHLHRTIQ
                     KIKSYGLKAGVSLNPATPISVLNEIIKDVDMVLLMSVNPGFGGQSLIENVKYKFYDLK
                     QLLNKYNLDIDIEIDGGVNYSNLKEILSWGANVIVAGSAIYHAEDIVLETKKFKEIMS
                     "
     gene            complement(34291..35169)
                     /gene="rsgA"
                     /locus_tag="PKH42_00990"
     CDS             complement(34291..35169)
                     /gene="rsgA"
                     /locus_tag="PKH42_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOA19031.1"
                     /translation="MIEGIITKGVGGNYYVDIGSKIIECRARGLFRLKNIKPLVGDKV
                     LIRITEEDEDMGYIEEIKERTSEIKRPPVANAQQLVIIFSVKNPEPSFILLDKLLIAA
                     KSNNLNPVICINKSDLANEEEKENILNIFKNTGYKIIFTSKYDEDSLQNLKDILKDRL
                     NVFSGPSGVGKSSLMNAIEPGFYLETGEISDKLKRGRHTTRHAEIFKLSFGGYAVDTP
                     GFSSFELEGIDEYSLKSYYPEIVKYDDGCKFLDCLHYKEPGCVIKEAVNSDLISRVRY
                     NNYIKLLEQIKESKPY"
     gene            complement(35182..37143)
                     /gene="pknB"
                     /locus_tag="PKH42_00995"
     CDS             complement(35182..37143)
                     /gene="pknB"
                     /locus_tag="PKH42_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOA19032.1"
                     /translation="MIGRTLGNRYEIIEKIGGGGMSNVYKAKCNVLNRFVAIKILRDE
                     LTQDSDFVNNFKQESLSAASLAHPNIVNIYDTGFEDDIYYIVMEYVKGETLKNYIKRK
                     GSLSEKEAVKISRQVAEALKHAHSHNIVHRDIKPHNILLTEDGTAKVTDFGIARASTS
                     STINNTSNVIGSVHYFSPEQARGGYVDEKSDIYSLGIVMYEMVTGTLPFDADNHISVA
                     MKQIQEKPMLPSKRVKNVKISKNYEDIIMKCLEKHQSFRFQNTDELLKKLDALNGNNN
                     PVKDETEIIDSPTIEIPALNDLEENVINIDLIDENSDKAFKSFFSGNEEDENVEKSIN
                     NKKITIAAILSALVIAVIGGVFLFKSFLYVPEVPVPSLLGRSEEEAKKLIEDLGLVFK
                     VSKREYSSEYDEGYVMEQSIDEGSKVKENYPIEVVISRGEKEIVVPNLIGKYAIEAGG
                     ILNNAGLSEGETTEENSDTYLAGQIIDQYPLPNTPARENDKVSYVVSIGPKIIYVKMP
                     NVLNLNLETAKLSIVQHGLAVGQVNEEPSEEIEKGLVMRQSITAGQEVEQGTSIWLTV
                     SSGKPEEPDEPTPEEGVFPLAISLPADRDSVSIVIQRIASDGREVVYSEEVDTSEGNI
                     LVNVKGKGTGIFEIYIDNELYDRVEITFE"
     gene            complement(37133..37885)
                     /locus_tag="PKH42_01000"
     CDS             complement(37133..37885)
                     /locus_tag="PKH42_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOA19033.1"
                     /translation="MDVFFDTNKGLMRENNEDSLIVEQFDNYCLYAVADGMGGHKAGE
                     VASSIAIEEIRNFFVECMKDENFQPPSFIMESVKLSNEKIREVSLNNEDCCGMGTTVT
                     MAVIDKLNNIIYIGNIGDSRAYLLRNNEIKQITTDHTYVNELIKDGRITEEEAKTHPK
                     RNVITRALGSEEYVHADIFELELLKNDILLLCTDGLTTHVSDDNMLEIIKEYGTSESV
                     QKLIKQANDNGGTDNITIIIVDNIIRGETYDR"
     gene            complement(37894..38922)
                     /gene="rlmN"
                     /locus_tag="PKH42_01005"
     CDS             complement(37894..38922)
                     /gene="rlmN"
                     /locus_tag="PKH42_01005"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOA19034.1"
                     /translation="MKKIDDLSFEELKDAVLKIGEPKFKANQIFDWIHNKTVESFDDM
                     TNLSQKTKAALKKEYEFTKAIIELKLESKLDETKKYVIKLDDSNIIESVYLKYKFGNT
                     ACISSQVGCKMGCHFCASTKKGFIRNLTAAEMLKQIYLIQNDINEKISNVVIMGSGEP
                     LENFDNIVRFLNIINDERGQNISMRKITLSTSGIVPNIYKLADENIPITLAISLHAST
                     QEKREQIMPIARKYKLPEILKACDYYVKTTGRRLTFEYIMIKGFNDSREDAENLSRLL
                     KNKLANVNLIPCNYVKEANIEPSEDLDIMKFKKILTDNGINATVRRELGSDINAACGQ
                     LRNDLLKM"
     gene            complement(38973..40292)
                     /gene="rsmB"
                     /locus_tag="PKH42_01010"
     CDS             complement(38973..40292)
                     /gene="rsmB"
                     /locus_tag="PKH42_01010"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOA19035.1"
                     /translation="MKSYRAQAIETLKKIFKDKSYSNIVINNDMKNVDNRFLSLYRKS
                     VLGVVENLIFIDWIINQVSKTKTKKMETDVLLVLRLAVYQIFFLDKSHENIVVNESVQ
                     YIKDNGNIRASKFVNAVLRNILRSKDNLIKKMNELPRDDYLSVKYSYPKELIVKWEKQ
                     FGKDVDKVLIANNTEAPLEIRVNTLKIQRDELIKLFEEKGIKSYKCKYADKGLIIENP
                     FEIDKTDEYKNGLFSVQSESSMLAGQILNPKENSFIIDVCAAPGGKTLNAAEMMNNTG
                     KILSRDIYPAKLRLIENEIKRLGIKNVKVEKYDARKLDESLVGKADYVIADVPCSGLG
                     IIRRKPEIKYNETDLKDITEIQYKILENASKYLKQSGELVYSTCTTNKEENIEIINRF
                     LKNKNFILADFSDIINEDFKTAKDGYIEIYPHLHGMDGFFIAKLKRL"
     gene            complement(40409..40780)
                     /locus_tag="PKH42_01015"
     CDS             complement(40409..40780)
                     /locus_tag="PKH42_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOA19036.1"
                     /translation="MEIKVIKINNVRIAKIYSNEILIKDGQSALDLLATVSYETDCDR
                     IIIDKSAIAEDFFDLSTKLAGEVLQKCINFHFKVAIIGDFSVYKSNSLKDFIYESNKG
                     KDIFFLPDENQAVEKLSIVRR"
     gene            complement(40818..41849)
                     /locus_tag="PKH42_01020"
     CDS             complement(40818..41849)
                     /locus_tag="PKH42_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOA19037.1"
                     /translation="MLKKPNRLNRGDKIALVSLSWGGAGDENLLWRYKQGKERLEQVF
                     GLEVVEMKHTLAGTEYIYNHPEKRAEDLMNAFQDNSIKGIIACIGGIDSIRMLPFIDF
                     DIISNNPKIFMGYSDTTTTHLFCYKAGISSIYGPTLLVDFAENVSMSPYTVEHIEKTL
                     FKSEAIGEIEPSPKWTSEYLPWEGKNKLIQRKFKDNSGYELLQGKGITKGRLIGGCIE
                     VFDSLRGTEVFPKLEDFDDSILFLETSEEKPPAWIIECSLRIYGINGILDRIKGMIWG
                     KPQDETYYAEYKEAIKKVMKEFGKEDLPILYNMNFGHTEPKICLPYGAMAEINCDKSS
                     FSILESAVL"
     gene            complement(41922..42554)
                     /locus_tag="PKH42_01025"
     CDS             complement(41922..42554)
                     /locus_tag="PKH42_01025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA19038.1"
                     /translation="MLDSKGFDLWAHGYDKSVNLSEENNEYPFAGYKDVLNYIYNQVR
                     KHNYSSVLDIGFGTGILTTQLYNEGYHITGIDFSRTMIDIAKSEMPEAILINWDFTRG
                     LPVEIKNNKYDYIISTYAIHHLPDEEKIKFIKSLLKLLNVNGKILIGDVSFMTRKELE
                     ECKETHTEIWDDEEFYFVANEMMMSLNEFYCNYEKISYCSGILIVADYLL"
     gene            complement(42595..42720)
                     /locus_tag="PKH42_01030"
     CDS             complement(42595..42720)
                     /locus_tag="PKH42_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19039.1"
                     /translation="MFGIISTESRYEFVGDSMEKLQDDYNLITGVYIGDVLDVHL"
     gene            complement(42708..43472)
                     /locus_tag="PKH42_01035"
     CDS             complement(42708..43472)
                     /locus_tag="PKH42_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19040.1"
                     /translation="MDNLERIAQFEKFSPMLYLDYKLTSWGRMLCDIGNKESYTSNYA
                     VIYRNENVDNIIKEVEEYYNSRSIIPKIFNRCGSVELDILRPFFTKHDYAIREFDMDL
                     MILDTHSTRNKIAEKVEVKIINRALEGQEYNLAIEQDNGETYGVKLLNKQIASGNNMF
                     FAYNELKKPVSMALAEKYNDVVYITDVYTTPSQRCNGYGTAVVYAVLSHYHNSLIYLH
                     TDNPDAASIYRRLGFSGKRLKSWWAVKGSLPEWCLE"
     gene            complement(43523..43756)
                     /locus_tag="PKH42_01040"
     CDS             complement(43523..43756)
                     /locus_tag="PKH42_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HOA19041.1"
                     /translation="MHHVTVNDESGREARLCDYDWVLDIREQYKKYDITFWFKGTGSL
                     FKHDGTIKKINPFKQGSHAKKFDINIKNSGDRA"
     gene            complement(43846..44619)
                     /locus_tag="PKH42_01045"
     CDS             complement(43846..44619)
                     /locus_tag="PKH42_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19042.1"
                     /translation="MINFNKLPLMIVIAVMAVVILSACDGVNSNSNETDDIITDENTP
                     TTETTAVPFPWGGNIDGKEMTLDDVHQLAGKGDDLLFEDLWQYKGGNTSSSHERYIMV
                     YKVEGGYRLVVHSDSTGKPDMVNLESIWESGGSGIDIRYNDVYEFLRANPSQDGITEE
                     EAQDIGQTKLTMELEPVSWYILGDWPESTDENAVFAEALLDSIYTIDEPCWVFRVKGT
                     AEWGGSYYAVGKKSGTIFICSFDDNSKPIWSVVDSESNS"
BASE COUNT        12787 a         9125 c         6198 g        16879 t
ORIGIN      
        1 tcatacacct caaatatttc cttaaccatt tatctgttaa aatatcaaac atatttgtca
       61 tcctgagcgc aagcgaagga tctcatgaga ttcttcgagc taaagctctc agaatgacaa
      121 aattgttatt cattgcaatg acaaaatgtt tttcatttgt ctaaatatct taacagcatt
      181 accgctgctt ctgctcttgt tgtattttgt gcgcctccga aaatatttcc gggcagccct
      241 ttgataattt cagcctttac cgtctttgca acgggtacct ttgcccactc gggaacttca
      301 tcactgaaaa tagacaaaat ttcatgtgca tttgttatat catcttctag caaagagcca
      361 attatagtcc ctatttcctg tcttgaaata ggagaattcg gtttgaaggt gccgtcttca
      421 tagcctttaa catatccttt ttctgctgct attgcaacat ccttagcaaa ccaatccgta
      481 gctttcacat cattaaattt aggtattgca tcttcattgc ccatacccag tacccttata
      541 agcagtgata caaattctgc tctgctgata ttatcgtttg gtctgaataa accgtcgctc
      601 ccttttgcat aacccattgc tgccatttct attatgctgt cttttgccca gtggctttca
      661 atatcactga aagtatcaat ttttacatcc ttcatgtcgg gtgcttcata atcccctgta
      721 cctactacta tacagtcagt ccatttaacc cacattgatt tgctttcttc aattatgagt
      781 cttaatggtc cgtatgcatt gttgtttaca tatccgatac ttgactcgtc gggtactaag
      841 ggatacccgt ctgccgcata agctaggatt attttcttag tctgaccttg agaatttaag
      901 actccgttta ctacctgttc gttgcttctt aatatttgat tgtaattttg tccgctgaat
      961 actcttatac ttggaacttc tacatcttct ttaagcccta ccacattatt tattaaatcc
     1021 cataaaacaa taccttgcat ttctcttatg ccatcgcctg tataggtatc tctaaccgca
     1081 tattcagtca ttgcctcaat ttgccttaag gtaaatgtca taggttgctt aacttgtgag
     1141 cctgttactc gcagtactgg catatccaaa tattccttat atacaccttg attgtgattc
     1201 catgattcat cgatttctat ttccgtgctg actccttctc caattccttg ggtatctgtt
     1261 gcaagagatt caccttcgcc gataattatt tcctttgcaa attgtacctg attggagccg
     1321 ttcgtatcgt tataatttgt tttcccgaat attattcgaa gaggtccccc gtcattgccg
     1381 agacctgggt tgtacccttc atcggtaggc cttattacat agggatattt gttaaatcca
     1441 tatgcaacca taacaggtaa attcgtataa agaggatcta catattctcc cgggaaggtc
     1501 ccgtcacctt tatccattgc acttttttca taatatccgt acagagacct gtccttaatc
     1561 tctttgatag tcattggtgc aaaattataa ttatctgcag caataaatcg tacgcttgta
     1621 ttttcgtcaa tgtcagggtc taaacccgcc tttaagagta aatcccataa aggaacaccc
     1681 ttgtatgtgt tataatacca gaaatattca ctgtttgaca aactgtattc atgtgtttca
     1741 tgaaggtctg tcattgcttc tatatctttt accttgtatc ttacttcttt tcccaaagca
     1801 gaaccatgaa ttatcaattc ataatctgta tatttttcag cattgtaagg agcatagctg
     1861 tgctcaaaaa tatttttggc atccttttct tctatgtaaa tatatgctac attggagaat
     1921 tcctcggctt tattcttgtc ttgttcataa actaatttaa aacaaccgtt atcgttatgt
     1981 ttttccttga tatagccttc atcagtatcc aagtaaacca ggggaacttc atttataccg
     2041 tatgcaacca tgtagttgcc tgcctccgat atgtcggcgg ttataattgt ctttcttgat
     2101 ttatctttaa atataatatt tccggcatta gactttgcct ttacgtaatt atcgattaga
     2161 tatttgacat ttattccttc ataggaaact tcaactacct taccgtttac tttttcataa
     2221 tatgtatcta ttgtctgtcc agttgtatct tcttctattt gccttaatga tatggctctg
     2281 taaccttcaa ctgcaggtcc ttcaattgta aaagttgctc ctgttacggc atccacattg
     2341 taaggctcac cttcatagct gatatgttta tatggtgatg aaggagttac ctttactatt
     2401 tcatctccta ccgtaatttt tataacttcc ttgccccatt tagacatatt catatcgtca
     2461 atgtcagtct gtccgaatac aagttttgga aaatctttat ctaaatcttt ttccgtaatg
     2521 ggaatatccg tccatgtaag aggaggagca aaaatatttt tcggaaaatc agccattacc
     2581 gtagtatatt ttgcaaccat aggcataact gcctgtttgc tttccaaagt aaaatcactg
     2641 aaataataag cgcttttaag ctcgctgata gtctttgtaa attcaaaacc gtcagagcat
     2701 gtaaatttta cgctgtagtc tttatcggtt ttcaaaccgt ttcccataag ttcaaataaa
     2761 tcatatcctt ttgcctttat gattttatga aatccaaggc tgttgttggt ggagtaaatt
     2821 ctctctacca tcttatattt tgaccagtca ccggatgaga ttactacttc ttcctccact
     2881 cccgtgcctg taattataag ttcaggctct gctgcaaata caaacgttgc attcattata
     2941 atcatggata caatcaagaa ccagactgta atttttcttg tcatttacct tacctccttt
     3001 aaaataaatg agcgaatatc gctcatttat tttgattatt ccgaaaacaa tgtattgaat
     3061 actaccaagg ctgcttcggc acgattaaca ttccttgcag gctcaaattt ttctactgca
     3121 atccctgcaa acacatcctg tgcttcaagc catgcaactg catttcctgc ccagtctgcc
     3181 acatcttctt tgtctatgta atcagatttt tccattacaa atttatcaac ttttgcttgt
     3241 tcaaccaatc cttttgcaac tgctgccctg gctgctacaa gtgccatttc ctgtcttgtt
     3301 attacctttt cgggtaaaaa tgtaccgtca ggatatccta caaacaaacc cgaatcaact
     3361 gcagcttgta catatggtgc cgaccatctg tcatcatcta tatcgctgaa tgctccttcg
     3421 tattctttca gctcatattc caatgccaca actataattt tgcagaattg ttctcttgta
     3481 aattcatttc cgggagcata gagtccgcct cccattccat caatgattcc tcttttggcg
     3541 aggtcataaa tagctgcctc tgcaaattta tattcttcgg ttatatcagt gaatgttact
     3601 tcttctgttt cttctgtttc ttcagtttcc tcttcaatcg gctcaatagt ctcttcttcg
     3661 ggtgcttctt cttcttccac tgcttctgct tcccctacag taattttatt aattagttta
     3721 taaacagtat caaattcatc ttcatatttt aaatttctgt atacagttat ttcttcaatt
     3781 attccttcat cattgttaat tgcctcaccg tctacctcat aagccaaaac aaatttaagt
     3841 tcatcgttta gaacatcctg aaggggctgt gggtcaatcg gccattcatc ggcagcgaaa
     3901 aattctacat ttccttcttc agcagttact cctgccattt ctttcaataa ataagctaaa
     3961 cttacaccct taacctggac tgttttttct cctcccttag aattgtaaac atactcctgt
     4021 gctatttgcg cttcctcggg catagatttt aaatcttcaa gggtaagctt aagttctgtt
     4081 gcagccaaac tacctccgat ttcaactgca taatcttctg caaaaaccaa tgattgcgaa
     4141 agaatcatta ctattgccaa taccattgca attactttaa attttttcat acattcctcc
     4201 taatagttat attttaattt agttagacta aatataaatc agaaaagtaa acgaactaaa
     4261 aaacagcact tctggatgga attgctgttc acatattatc tagtttctcc tttccaccag
     4321 gaggcttgac cgtttccaat caaaccctat cggcataagg ccataaatat cattcttagg
     4381 ctgttatgct cggagttatt aagtttatat taatttaatt ataacataat gtaaaagaag
     4441 tgtaaatacg ccaatattaa ttaatcttca aagaccttac cgatgctgtc atttataact
     4501 aaatcggctc ttctatccat tggagttgat gatttgttaa tgagtaccaa tttgcttccc
     4561 ttaaagtaat taataaggcc ggcagcggga taaactacaa gggatgtccc nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttaaaaaaa tcattttatt tatataaaaa
     4741 tcaaagaact cctcggtatt actcctgaag aaactgtggg aaaccatcat ttcaggcgga
     4801 tatttatatt ttttgctgta caatccgtca acactcctga aatccggtat accactttcg
     4861 gtcgaaacac ctgctcctcc aaaaaatact atattatcac tttttgatat tatttctttt
     4921 aattcagcta tcataacaac atctccctct tattattctt ttaaccatta tactatattt
     4981 ccttatttct ctcaaaccta tcgcaataaa aaaggagcag agctccctat aagaagagat
     5041 ccttcgctta cgctcaggat gacataatgg agtatgccaa taacctgagg agcagagctc
     5101 cccttgtcat tattcgtaaa tatttactat tctgtcccct acaatatcca tgttaaggta
     5161 gtcgacatgt ctttttggaa tggttacaac atcaggattg ccatcgctta taaatctttc
     5221 tcttttgttt tcctcatatt gtttaaaata atcgttaatc ccgcaattat ccacattaat
     5281 ttcacttgca gttacacggt agaggttact gaagtctgtc aatatatcaa aaggtgaata
     5341 ggcaggtctc cagcctacgt ccagaactgc aagggtccta tatttattgc cttcatattc
     5401 tccttcaagc accgattcta taaattcatc ttttggattg ctgccgtgag gaagagccaa
     5461 tgtttctact gcataatcgg gcaaatattg ttttattatg ttgtttacgg tgcctatttc
     5521 aattattatt tcatccgaat ttaattctgc catctcatta tggctgtagg tgtggtttcc
     5581 caaatcatat ccgttttcaa taagccaatt aagttttttt tctgcatatt ctttttgtcc
     5641 gaacaaatta gttcccaaaa aaaagctggc agttacatta aagtcgggat atttattttt
     5701 aaattcttcc aaaatcccga tggcacagtc aggatctatt acaattttcc cgttttcttc
     5761 aatataactg aaattatttt gccttccgtc gtcaaaggta agtattattg gagtatatcc
     5821 tgcttctgtt ttgatttctc ccttagcata atcatttaaa cttatcattc ggtaaccgtt
     5881 ttgatacatg tactccaagt ccttgcgaaa gttatccgga gttctttgcc atactgattc
     5941 ttccggacct attccatgat acatcaatat cataatttcc ccaagctcat tagggttcaa
     6001 cgacaaatcg atggaagcaa gaatttcctc atcgcttagc tctttgtctt catttaaatt
     6061 atcggtatct tctttatctt cttcagggta ttcttcaccc ggagcttcga cggcaggggg
     6121 gttttcagga atttctgctg tgggagctcc acatcctgtc atcattatta atgctattaa
     6181 aataattatt tttttcattt gccctcctct ttccttatta ttatactata ttatttaagt
     6241 ataaatgtct acatgaagta ttttcaaatt ttagtttatt attaactgca tgtttgatat
     6301 aataggaaaa gatgaagagg gacacacctt cttattttag ggaaagtctt tgcttgtaga
     6361 aggaatgtcc ctgtacttag ggggatgaca gtcttcgtcc ccggaataat tatatgatta
     6421 atggagggca aaattgataa gcagcaataa aagtacccaa gataagctta aacttttgta
     6481 tatattggat tatattgata tacctttgac aaataatgaa attactaatt atatattgga
     6541 atatagcatg atggattatt tcacattgca gcttttgtta agtgaccttt gtgaagttaa
     6601 gtttatacac cttaagcctt ataatggaag tgagtattat tccgtctcaa agtcaggcaa
     6661 ggctgcctta gaaatgttcg gagataaact ccccgaatat tttattaatg aagtagcaaa
     6721 gaattattca ggcataaaaa aacatataga aaagcaaaga gaaatattcg gccattatta
     6781 taaaagaaaa gacgatgaat atattgtaac tcttcaagtt tcggaaaact ccacaactat
     6841 ttttaattta agcattaatg tgactgatga gattacggca aaaaaaataa ttaaaaaatg
     6901 ggaaaacaat cctgataaaa tattcggaga aattattaat gcattaacaa attaaggtat
     6961 aatctttatt aaaccggggg taaacattag ttttaaggtg cttatattat gcccttgttt
     7021 ttctattata tcaatttgct gctttttaga attagtaaca taaatatttt tgctgccgca
     7081 agaaatggat tcaggatatc ctcccaaata tattttttta ataatttttc ttaataagga
     7141 atcagcaata taaagacttt tatcaacaat atttattata tacagcatat tgttttcata
     7201 atccagtaaa aaatctgaag caagaccatc tatgtcgata gtatcttcaa tttctttatt
     7261 tgataaatta atgaaattta ttttagtttg tacaatccca ttgacataat aagtcattac
     7321 tattatcgta tcatccagca caaatatatg catgggcttt ccatccgttt tgaatcgtct
     7381 tcttattcca ttgtcaatac tgtattccaa tacttcattt tcttcgtagc aggttgtgta
     7441 aagcgacttg tttttttctg aataaattac gtcatgtgct tttatgcctg caggtaaaga
     7501 gcctgtaagt gtaaaggaat ccagctcaat gattgaaata ttatccgatt caaaatttgc
     7561 tacatatata ttatctttgt ccgtgcacat atgccgcggg tctcttccca cattggtttc
     7621 aaaaaaatta tttacgctgt cgaatttgta aattttgtta ttaaaggaat ctgtcatata
     7681 taccgcatca ttaaaaactt ctatgctgtg gattgcaaag ctgtcgcctg ctcccaaata
     7741 taaagctttt ttaaaagaat aatccttttt taaaagctcc attataaaca aaaataattt
     7801 tccctgacat atgccggttg tgacgatttt ttcatcatac actgcaatca cctccatata
     7861 gtttatgaat cacaacaaaa gaagtgaata tttttgataa attcctcaaa tgatattaga
     7921 ctaattaaaa cacagcagtt ttcactgctg tgctgatttt catattatcg tgctgtcggt
     7981 atacacactc ttgttcctac aatcagaaag tttgccggtc tcaaatgagg atttgcaata
     8041 agtaattctg aaggtgtaat attatttgca attgccactg tagaaagact gtctcccgcc
     8101 tgtattacgt attctgtggt tgttccttca ggacactgca taaatgtttc ctgaggtatg
     8161 catagttcag ttccgggtac aataatatta ggattgaaat taacatttgc atctcttaat
     8221 tcatttaagc ttaaattgta agatatgaga atatcactta accttgttcc ttcttgtata
     8281 gtcacaaccg taccgaaagg acaccccgga ggaacatcag gtatgcatat ttcctgacca
     8341 acttttaaat catagggatt tgcttccgga ttggcttcta agagcaaatt caaaggaata
     8401 ccatatcttt gcgcaatatt ataaaaacta tctccttccc ttatggtata gtaggctcca
     8461 ttaacacatg gcggtctttc aggaaccctt ggcacacata tagtcattcc ttccgttaat
     8521 gaccttaatc ttaatgtagg attcaattca atcagcccta ctaaatttgt accaaatctt
     8581 gctgctattg aatttaaggt atctccgggt cgcacctcgt atggcattga atatatatta
     8641 gggcataatt gctgattttg tctataaatc actataatca cctcatatta taatttgtta
     8701 gtttataata ttcatgataa agtgaatgtt tccataatat gtatctttac tttgtttcaa
     8761 atttttttct ctcttcaaca gctaacttat cacatctctc gttatagctg tgccccttgt
     8821 gcccgggtac atgaacaaat tttactttat ggccttccat tgcctttaat agtctttgcc
     8881 ataattcctt gtttaaaact gctttaccgt cagatttctt ccaagacttt ttctgccatg
     8941 catcaatcca tcctttattg aaggaatcag caatatactt tgagtcggtt acgattgtaa
     9001 catcgcaggg ctcttttaat gcctcaagtc ctgtaataac ccccataagt tccatttgat
     9061 tgttggttgt attttctgaa ccgcccgaaa gctcaacttc atgtcctttg tattccaata
     9121 ttacaccata ccctccggga cccggattct tgctgcaggc tccatccgta tatatgttga
     9181 cttttttcat tttttggacc tcttattttt aataagcatt actcttagta tgccgtaatg
     9241 ctctactaat ccatttaaca tatcttcatt gagctcaccg attacgtcaa ttatattaca
     9301 agcatagtca ccttttgatt tattgaccat ccctattatg ttaacattct gagatgcaag
     9361 tgcagtggtc attttaccta tcatatttgg aatattctta ttcaaaacaa caatcctgtc
     9421 cgttcctgga tacctgtcca tggcacagtc gggaaaatta acggaatttg taatgttgcc
     9481 tttttccaag tattctgtca actccgtaac agccatttcg gcacagtttt cttccgcttc
     9541 gattgtagag cctcccagat gaggaataca caaggtattt tttgctttta aaagtttttc
     9601 gtttggcagg tctgaaacat gataacttac cttgccgctt tctaaagctt ctattaagtc
     9661 atcctcgttg attattccgt gacgggcaaa atttaatatt tttacaccgt ccttcatcaa
     9721 tttaatatta tctttattta tatagtcctt tgtttcatta ttataaggaa catgaatggt
     9781 tatataatcg cttcttttat ataaatcatt aatatcaccg gtatattgaa catcccagga
     9841 taatgagagt gcgtttttta ctgaaatata agggtcatat ccgataacct ccatacctag
     9901 tttagtcccc aggtttgcaa caagatgtcc cacatttcca agtcctataa ccccaagagt
     9961 tttccctttc agctccggtc ctacgaattt ggatttctcc ctttccacgg tttcaacgac
    10021 gttgctttcc agcttctttg tccaatctat actgtctatt acttttcttg agctcattaa
    10081 cagtcctaaa aaagttaact cgcacaccgc gttggcattt gctccgggag cattgaatac
    10141 gacaatgcct tcttctgagc atctttcaac aggaatgttg tttactcctg ctcccgacct
    10201 ggctatggct ttgagctgtt ttgaaaactt catatcatga atatccaaac ttcttattat
    10261 aattccgtca gcttctgatg tttcttctct tattaaataa tatttatcct caggaagcaa
    10321 atttaaagtt tcctggggta tgtcacaaaa aagtgctact ttaaacattt tgtcaacatc
    10381 ctcctctata ttaaagatta gtatacaacc tatataatag aaaatcaagg gataaaaaaa
    10441 atacggcctc taaagccgca tttcagtaag tactatctgc cgaatcctct tccgaagaaa
    10501 ttgtttcctc ctaacaagaa aaatataatc cccaatataa ttaaccattc gaaccaatca
    10561 ttagatctgt catcgcagca tctgaaatta gttgctctta cttcatctga catataaatc
    10621 acctctctta atttataatt caatatatgt cagataaaaa aatatgtgat aatactgaaa
    10681 cgttttatta tttttaatta ttatattatt aatatttgtt gcataatttg gaattcaact
    10741 catggacgta gccctcgtct tcctgagccc agcgaatgtc tcatgagatt cttcgagctg
    10801 aagctctcag aatgacaaaa atgttgttca gcaatgacaa gtttcattat tataaaattt
    10861 ctaaagttaa taagactttt tcgattaatt cttcgacatt taaattgctt tcagaccttt
    10921 taatactctc aagcttagga tttattaaag gatgctgtgg agcaaataca gagcagcagt
    10981 catcatatgg caaaattgaa gtctcatagg tgcctatttc tcgagcaatt tctattattt
    11041 cagatttatc taatcccacc aggggtttaa gaataggcat attgattgca tcttcaataa
    11101 cagccattga cttcattgtc tggcttgcaa cctgacccag gctttctccc gtaataagca
    11161 tatcgatttt attttccaat gcaatttttt ctgcaatacg catcataaaa cgccttgcca
    11221 atatagtggt ttcttcttct ttgcaaaatt ctcttattgc tttgtgaatt tcaagcatgt
    11281 taatactgta taattttatt ttcccgcaat attcttcaag tttttgtttc agcttaatta
    11341 ctttttcatt tgctctctca gacgtaaaag gatatgtatg aaaataaagt gcatctattt
    11401 caacacctct ttttgcaatc atatatcctg caacagggct gtcaattcca cctgaaagta
    11461 atagcagtgc tcttccgttc gagccaaggg gaagaccgcc caatgttttt attctttcgg
    11521 aagatatata gcagcatttt ttaatatcaa tatatacttc cacatcgggg gtatggacat
    11581 caacttttac aaagctgaaa ttctccaaga ttttaccgcc cactaaggca ctgaaatcca
    11641 ttgatttaag gggaaattcc ttgtcgcttc tcttggtttt aattttaaat gtttttatat
    11701 cctccttact taacagatat ctaaacagtt caattgtttt ttcaataatt atatctacat
    11761 ccttgcttgt tttaacggaa ggacttatat tggatatgcc aaaaattttc cttacctttt
    11821 cgactatttt atccatatcc tcttcatttt tcgtttcaac atataccttg cctgaatctt
    11881 tgtatacgga cacatgatta aattctttca gagcatattt gatttgctca atcagctttt
    11941 gctcaaattt tcctctgttt tttcctttta aaaataattc tccgaagctt actgcagcta
    12001 tattgtacat attatcctct ctttgttata cttcttatat ctttaaccga ttttttaata
    12061 ttatcaatta cataatcgac ttcttcaaag ttattaaaat gaccaagact gatgcgtata
    12121 gttcctgccc ggtattcctt gcttagtttt attgcttcca atatcctgct gtcttttgat
    12181 tttgaagagc aagcagagcc ggttgaaaca aaaatatttt tatcttccaa ataatgaacc
    12241 aatacttctc ccggcacatt tttaaatgat acactaataa tatgaggagc tccgtctata
    12301 ggactgttta ttttaatatc ttcaatttct tcaagccttt ttatatatgt attttttaaa
    12361 ccgtaaagct tatttacttc ctcatcaaaa ttatcataca ttaatttaca agccttgccg
    12421 aagcctataa ttcctgttgt gttctcggtt cccgagcgaa tccctctctc ctgtcctcct
    12481 ccgtaaatta atggagttat ttttataccg gagcgtataa ataatccgcc tactccctta
    12541 ggcccgtgta ttttgtggct gctgaacgac aaggtatcaa gaggagtttt atttacatct
    12601 attttaattt tcccatagga ttgaacagca tcaacatgca gttttgcatt tttgtttttt
    12661 cgctttataa ttgccgtaat ttcattcaat tcctgaactg ctccaatttc attgtttaca
    12721 tgcataattg atatgaggag ggtattttca tccaccatat tatgaagctc atcaatatta
    12781 atttttccgt tttcatccgt ttctaaatac ttaaccaaac atctgtcttc taactgttta
    12841 aaaacattgt atacggaagg atgctcaata ttagtggtga taacattatt gtactccttt
    12901 aggtttgata aatgtccaaa aattgcaata ttattgcttt ccgttcctcc gccggtaaaa
    12961 taaatttctt caggttttgc acctatgagt tttgccgaaa gtgttcttgc ttcatcaatc
    13021 ttcttttcaa ccttatgtcc catcctgtgc agggaagaag gattcccgta ataattttca
    13081 tgagcatcta ttatttcttt caatacttca tctcttactt ttgtcgttgc actgttatcc
    13141 aaatatacta acatatttac ctctttgttt atttttataa gtttctttta agattctttg
    13201 acaagccctt catctaacgg tgccaggcac catgctgagg tgccaggcac ctggagaagg
    13261 cacctaggga actcgaaatt tgtaaaaaaa gcttgcataa taattttatg tttgctataa
    13321 ttgttattgg ttatcccccc cgataaccaa atatattccc aatacccctt ttatgcatat
    13381 taatgcttca caacaatggc caccaggcca tttttgtgtt tatgacctaa cccttctcaa
    13441 tctctttttt aacctctcct aaatataatc tggacacctt cttcacaact tcattttcgg
    13501 aagaattaaa gacaatatta tcaacggttt ttatcggaag tacatcattt gatgtacctg
    13561 ttaagaatac gccgtcgaaa ctttctatct cgtccaatct aattatcctt ttatgaacct
    13621 ctatcgaatt ttcttcacaa acgcttataa ccttacttct tgtgacgccc aaaagaacag
    13681 aagaatccgg cgatgttatc agcttattgc cttctacgaa aaaaatattt gatttgcttc
    13741 cctcgctgac cgtgttgtca tgatttatta atatggcttc aaaagcatta ttttctttaa
    13801 ttattcttgt aactttatcc ttatattctt tttggaatga cttaacattg gggtttgctc
    13861 tctccatttt caccgtaacg gtatgtactc cttcagcaaa ttcgtctttg gaaggatagt
    13921 aactttcaat aaagtagaac aaagtaagag gctttttatc ataaaagtaa gaaactctaa
    13981 tgtttaaatt tttgaaggaa ttctctttta taagcaattc tacggaattt ttgaactctt
    14041 cataatttat gtttccttct attccgctta aatttatgct ttcgctcata cgttggaaat
    14101 gttcctttaa aaaaataggc tttccatcaa taacccttac tacctcataa attttagtct
    14161 tatttataag aagctctgtt tcttcctttg taataatttg attgttcatt acatttttat
    14221 cggataatat attcatatgt cctcctatac tacaacaaat tttttatatc ttctgaagtc
    14281 ttaattttct taaaatgttt ccagtggtcg ggaaataatt tcaaatatgt atattgacaa
    14341 aatcttgagt caatcaacag gactgttccc ctatcatctt cagttcgtat tactcttcct
    14401 gctgattgaa ggactttgtt gaagccggga tatttatatg cataatcata acccgtgttc
    14461 attttttcat caaaaaatgc tttaattata tttctttcaa aagaaatttg cggtatcccg
    14521 gttcctatta taactgcgcc aattaattta tcaaggggta aatcaattcc ttcagaaaaa
    14581 gtacccccta caacagtgaa tagaaccaca ttgcgctcat tttcgaacct gcttaagata
    14641 tccatctgtt cttcttcatt gagcccctgg ctttgaacaa ttatgttcat ggtatcatac
    14701 aatgattcat ataccgaata aaccttattc atgaaattat aagaggggaa aaatatcata
    14761 taatttccct ttttttctgt tatcaaagta ttgatatatt cacatgtctt ttcaatattt
    14821 gaatccctct ttgtgtattt cattgatata tctgaagtta tcattaagtt taaattctct
    14881 ttatcataag gagagcttaa ctttaatata taatcatctt cctctccccc taatatatat
    14941 ctgaaatatt tcaagggagt taaagttgcc gagaagaaaa ttgatgaccg ggcattcctt
    15001 tcaatttcct tcaatatttg ggaagtatca atcaagaata atttcaccat tgttttattg
    15061 tttgcagaat cggcataata gcaaaaatta tcatccgcaa tttccgatat ttttataaaa
    15121 aaaatactct taaaatatat gtcggtaagt tcctccggaa ttttttcatt ttttctttcg
    15181 ttgaaatatt tatcagcctt agcgataaat tttcttaaag catttattaa ttcttccgga
    15241 gtttctttta atatttgtgc ttcatctata ctctttttaa tatttataaa ttttttatta
    15301 atattaaata attccttgct taagcttttt tcagatttaa taattttata tacttcataa
    15361 aattcttcct tgattaactc cggagaatac atatttcgta ctcggtcttc aagattatgc
    15421 gcctcatcta tgagcagtat atccttgtcc ttattaaata gatcctctct tttcagtgct
    15481 accctcgggt caaaataata gttgtaatca catattacta tatctgacat ataggacaga
    15541 tcaaggctca gttcaaaagg gcacagttca tacttttttc ctaattcttc aataaactca
    15601 cggtcataca tgcaattatt ctttattgtt tctcttaatg gaatattaag cttgtcataa
    15661 taaccctttg cataagggca gtattccggc tcgcattttg tttcatccat gaagcaaatt
    15721 ttgtccttgg cggttataac ggttgttctc agcctaagac cctgtttatg catcatttta
    15781 acagtgttga aagctatagt ctttgttgaa gacttggccg taagatagaa tattttagca
    15841 tttttttcat acagggattt tattgcagga aataaaacgg atactgtttt ccccactccc
    15901 gtaggtgcct gtgcaaaaag tttttttcct gaccttattg ataaatatac agctatcgaa
    15961 aaatctcttt gtccttttct atatttttca aaaggaaaat ttaattcctt tattgtcctg
    16021 tttctttctt ttcgaagatt tataattgtt tctgcccaat caatatatgt attaagaaga
    16081 gaataaaaaa atatttttaa ttcttctgtt gaaaatgtct gctttattgt tttcacttct
    16141 ttggtgtcaa gattatagta cctaagctgc acactgatat tttccaaatt gtttgagagt
    16201 ccgtacatat aaccgtagca tttagcctga gccatatggg cggtgctgta ttcaccatta
    16261 atcaaactta aatccgtata agttgatttt atttcatcaa ttattgtttc atcgtcttca
    16321 attaaaattc cgtctgctct gccttcaatc aaaaatataa tatcgttaag gacaaattcg
    16381 cctttcagat agtattcttt ataataattt tctcccattt gattctggag aattttatgg
    16441 gctttaattc cttcaattgc tctatctgca cctaaatttc ttacattaat atcgccgctt
    16501 ttataaatga attcgaccaa ttctctgaca gaaagtttta tattgtccat tattactcct
    16561 tgaacaggga catcggtgtg tccccatacc ataaaaaata aacactcgta gtgtttatta
    16621 tagcaaattt tgtatgttat ttaaataatt attttaaata ttgtacacaa gtaaccgtat
    16681 ttccttcaat gcttaacttg tcggaaagtc cttccaccag cattaaacct cttccgcatt
    16741 ccaaataatc atattccccg caatttttat gctcatagca aatacctgaa ccttcgtctg
    16801 aaacctcaac aataattctt gattcgttaa tatagacttt tatatatatc attttattta
    16861 agtctccaca attaccgtgg acgacactat ttatcatcag ttcattaaga ataagttttg
    16921 tgttgaaata ggtattgggg tcaatcagtc cctcaatgct tccaagaatc tcttcgatta
    16981 tcttcttgcc gcatttaatg tcgctattaa cttttctttc aactacataa tgcataataa
    17041 ttcctcctcg tcataatata ccctgtttta atgagcttaa acagcaaaat atattataac
    17101 acaaaggtat tcttattttt tacaggggtt tgtctaatca tgtaaattta agatgctttc
    17161 aaatagtatc catatttctt ctactccaag tttggatgtt gatgaaaatt gatatattaa
    17221 acctatatcc tttatttgta aggttttttt aatattattc aaacttttaa tctgttggga
    17281 cttagataat ttatctgctt ttgtagctat aatatatcct ttgaacccaa agcttttaat
    17341 ccattcgtac atcagcaggt cgtttctgcc cggctcatgc cttatatcta caagcagtat
    17401 aacttcctta agctgttgtc tgctgtgcaa gtataaatca atcatatctg cccatttagc
    17461 ttgttctgcc tttgatactt ttgcatagcc atatcccgga aggtccacaa agttgaattc
    17521 atcatttata ttgtaaaaat tcaaggtttg tgtttttccg ggctgtgagc tggttttcgc
    17581 aagatttctc ctgttgacca aagcatttat catagaagac ttgccgacat ttgatttgcc
    17641 tgcaaatgct atttcaggaa ctccagtatt cggatactgc tcttttttaa ccgctgaaat
    17701 caaaaattca gcttttctga taatcatata tatcccttct tattcttggc caaaaccgtt
    17761 agcggatttt ttatatctta tcgcaacgct tctttcacca catcatctat tttatctgca
    17821 tatacaattt ccattgagcg aattattttt gggtcaattt tcgaaacatc cgccttgttt
    17881 ttagagcaga gtaaaacttt tttaattcct gctctctttg cagccaaaag tttttcttta
    17941 aggcctccga tgggaagaac tcttcccgta attgtaattt caccggtcat cgcaacatct
    18001 tttctgacag gtttctttgt caaagccgat attattgctg ttgccatggt aattcctgcg
    18061 gaaggaccat cctttggaat tgcaccttca ggcacatgta catggatgtc cgtttcttta
    18121 aatattttat tgtcaatatc gaaattttcc gcattggctt ttatatagct taatgcagcc
    18181 attgcagatt ccttcataac atcgcctaat tgtcctgtaa gctgtagttt cccgtcaccc
    18241 ttcatggtat tcacttcaac aaacagtgtt tctccgccta cctgagtcca agcaagtcct
    18301 gttacaactc caacctggtc ttcctcatag gcaatatcat aatcatattt agggatgcct
    18361 aaatattttt ttatgtttaa attatttatt gaaacagtgc ttttgttttt ttctactatt
    18421 tgaaccgcag cttttcttat taatttacct atatttcttt ctaagtttct gactcctgat
    18481 tctcttgtat atctttcgat tatttcattt attgcatttg ttgaaatttt tacctggttt
    18541 tctttaagtc catgctcgga aagttgtttt ttaattaaat atttttctgc aatatttctt
    18601 ttttcaatat ctgtgtatcc tgatatttca ataacttcca tcctgtccaa aagaggaccg
    18661 ggaatggttg aggtcgtgtt tgcggttgta ataaacatta cattttgcaa actgaaaggt
    18721 gcctcaatat aatggtcggt aaatgtattg ttttgctcag ggtcaagtgc ttcgagcagt
    18781 gcagaagccg ggtcaccttt aaaatcactg tttaacttat ctatttcatc caataaaaat
    18841 acgggattat tcacttttac ttttgcaatt gatgatatta ttcttcccgg tattgcaccg
    18901 atatatgttc ttctgtgacc cctgatttct gcttcgtccc ttactcctcc caaagaaatt
    18961 cgcacatact ttctatccat ggcgcgagca atggattttg caattgatgt tttccctact
    19021 cccggtggtc ccacaaagca cagtattggt cccttcatat cctttttaat attctttaca
    19081 gctaaatatt ccaatattct ttcctttact tcttccaatc cgtaatggtc ttcagcaagt
    19141 atttttctgg ctctgcttaa atcggtattg tcctttgtct ttttattcca aggtaaatct
    19201 aaaagccaat caacatacgt tcttataacc ccggcatccg gtgaagatgg agatattttt
    19261 aaaagtctgt taatttcctt ttctactttt tcttttacat ctttaggcat gttggccttt
    19321 gagatttttt gaagatactc ttcaacttca taatccgtac cttcatcttc ccctaattct
    19381 ttttgaatag ctctcatctg ctcttttaaa taataatctt tttgaaactc gctgacctgt
    19441 tttttaacaa gctcgtttat ttcattttct atctgcagca ccttaatttc tttcgctaac
    19501 attttattta tggattcaag cctcttataa gggtcaaaaa tttccagaag agcttgtttt
    19561 tgatttgttt tcagataaat atatgaagca acaacatctg caagcttatc gggatttttt
    19621 atttctttaa gtgatataac ggcatccggt gcaatttttg cataaattaa agcatattct
    19681 tcaaaattgt ttaatgtaag acgaaccatt gcctccaaat tatcatcaat aggaagactg
    19741 tcgtcatata tatactcatc caaagcaact tcaaaatagg ggtcatcctg ggttacagat
    19801 acaattttag ccctgtttat accttcaaca aggactctga tattgtctcc gggcatttta
    19861 atcatttgct ttattttaca aataacgccc acttcataaa aatcatcttc cttaggcgac
    19921 tccatgtccg caaccttttg tacggtcaaa aaaatatctg aatcttcaag cattgcttct
    19981 tccaatgcat ttatcgattt ttcccttcct atgtcaaaat gtattacggt attggggaaa
    20041 attcctatgc ctcttatcgg aatcagcgga aggtttcttc tctcaatttt ataattctta
    20101 atcatacttt ctccttaaaa aactgcctgc taatggcagg cagtctatga aacattctct
    20161 ttcttatcag aaacaggctt ctttgttaat actaatttcg gctcactttt atttataatt
    20221 gtgtctttag taataacaca tttcttgata tcacttcttg atggaatttc atacattact
    20281 tcagtcattg tgctttcaag aattcctctt aagcctcttg ctcccgtatt gatttccaat
    20341 gccttcttag ctatttcttc cagagcatct ttctcgattt ccaactctac gttatccatt
    20401 ttaaataatt ctttgtattg tttgactaag gagttctttg gttccgtcag aattgatatt
    20461 aaagcatttt tatctaattt ttccaaagct acaattaccg gtattcttcc gacaaattca
    20521 ggaatcattc catacttcaa taaatcctgg gattgaacct gttttaacaa ttcatccgta
    20581 tccattgagt tgtttcctga aatatctgct ccaaaaccaa tgcttttctt acccactctt
    20641 ctttgaatta ttttctcaat accatcaaat gctcctccaa gtataaacaa tatattggtg
    20701 gtatctattt gaatgaattc ctgatgagga tgttttcttc ctccctgggg cggaacattg
    20761 gcaacagttc cttcaagtat tttaagcaat gcctgctgta caccttcgcc gcttacatcc
    20821 cttgttattg aagggttctc tgaacgtctt gaaattttat caacttcatc gatatatatt
    20881 ataccctttt cggctctttc tacatcaaag tccgctgcct gcaataattt taaaaggata
    20941 ttttcaacat cttcgcctac atatcctgct tcggttaaag atgtggcatc agctatggca
    21001 aagggtacat ttaataattt tgccaatgtt tgtgccaaat atgtttttcc gctgcctgta
    21061 ggtcccaaca atagtatgtt gcttttttga atttctactc cgtcatcttt agttttgtag
    21121 ttaattcttt tataatgatt gtataccgct acaaccaaag cttttttggc gttatcctga
    21181 tttattacgt actcatccaa ttttgccttc atttcttttg gttttggcag ttccgtaaaa
    21241 tcatattctt caaataaatc tatatcttca tcaacaatgt tatagcataa ctctatacat
    21301 tcatcacata tataaacatc cggaccggct ataagtcttc ttacctgctc ctgagacttg
    21361 ccgcaaaacg agcatttaat atgtttttca tttactttgc tcattgaaac actccttatt
    21421 ttcttttagc tataatatca tctattatgc catagatctt agcttcctcc gcagtcataa
    21481 agttatctct gtctgtatcc tgttgtacct ttgctatcgg ttgacctgtt ttttcgctaa
    21541 taatcttgtt taatgtatct cttatcttga taattctatc ggcatgtatt tttatgtctg
    21601 aagcttgccc tctcatgcct cccaaaggct ggtgaatcat tatttctgaa tttggaagag
    21661 caaatctctt tccttttgct ccggacaaga gcaggaatgc tcccatactg gctgccatcc
    21721 cgacgcatat tgttgaaaca tcaggtttta ttaactgcat ggtgtcataa attgccatgc
    21781 ctgcagtaat ggagcctccg ggtgaattga tataaaattg tatatcttta tccgggtctt
    21841 cagactccaa aaacaacatt tgtgcaacta ttaagcttgc cgtcgcatca ttaacttcat
    21901 cactaagtat tacaattcta tcttttaaaa gtcttgaaaa tatatcgtaa gacctttcac
    21961 ctctaccggt ctgttcaatt acatatggta ttaatgtcat ctttattcct cctatattat
    22021 atttatttct accttaattg tacttaattt tcttaaattt atcttaatct tattctttgt
    22081 cttcttccgg ctcttctgct ttttcgcttt cattaattgt ttcatgagct tctattgatt
    22141 caacagtctc aggagcatcg acaaaaaccg cattttcaac aagcatatca aagacttttc
    22201 tttttttaag agtttcttca atatagcctt tgcttgactg cattaaactt tctttaaatc
    22261 cttcaagtct gtcttcttca accttgtatg actctgctat tttatttatt tcctcttgta
    22321 attcttcatc tgaaactgtt atttcttctt ttttaataat ttcttctaaa accaattctg
    22381 cctttacatt gaactcagcc tgacctctgg cactttcctt atattgatta acaaaattat
    22441 taatcaactc gtcgtattct ttgccggtaa atccttgctg acggaactgc tgctcgtaat
    22501 ctcttcccat atgctcaatt tccctctgga ttaaaacttc gggaacttct aactttgtat
    22561 catttacaat ttttgttata actttgttgt cgtttgagat tttagattgg tcggcagcct
    22621 ttttttccaa tacttttctt gtatcctcta tcagctcctc caatgtatca aattcactta
    22681 catccttagc aaattcatca tccatttcag gaagttcctt tgctttgatt tcatgtattt
    22741 taacattgaa aactgcatcc ttgcctttta aattctctgc atgataatca tcagggaatg
    22801 ttacattaac tgttacctca tcaccttttg acttgcctat caattggtct tcaaaccccg
    22861 gtataaaaga attcgagccg atttccaatg tttgattttc ggctgtaccg ccttcaaatt
    22921 gttcttctcc gacaaaaccg gcataatcaa ttgttaaaaa gtcgcctttt tgaacaggtc
    22981 tgtccgtaac ttcaactatt ctcgcatttt tttcctgtat tgattttaat tctctttcta
    23041 catcttcgtc agttactttt atttctgtct ttacaacctc aattcctttg tacttcggca
    23101 gttcaacttc cggcattact tcaacttcgg ccgtaaatac tatttcttca ccatcttcaa
    23161 atttttcaac atttatttta ggactgtcta taatgtctaa ttctaattca ttaacagctt
    23221 tcggatatgc ttcctgaaca gcaatatcca aagcatcatc ataaaaaatg ctctttccat
    23281 attttttttc tataatatgt cttggagctt ttccctttct gaatccctga acatttatat
    23341 tattcttaac ctttaaatat gctttattca cagccttatc aaactcatct tgagatactg
    23401 taaattctaa tgtaactgta tttttctctt tttttattaa ttttgaactc atttagccct
    23461 ccgttatcct taagtttcta tgtatcattc aattattgac cttagattca tgaattaacc
    23521 attatattat aacataaaac atctttaaag caacctttat ttttggggac attcctcgaa
    23581 cttgcaaaga ctttccttat actagaggtg tgtccccata cctctgtact catataatct
    23641 gcatttcatt attatattat cgctgtttaa aaaaatgtga gttttatttt ttacaaattt
    23701 cattcctgcc ttttccataa ctctccctga tgcttcattt ctaatatcat gaattgcaca
    23761 aactttttca aatccaactt cttgtgttaa ataatgcagt atactgatta atacttccgt
    23821 agcaattcct tgattccata gtttttttgc tatagtgtaa cctacttcac aatatttttt
    23881 ccacttttta atgtttgata cggtaactga tccgattact tcttttgttt ccttgtccac
    23941 aacagcccag tgcaaataat catttttttt ataaaggctc gtccattccg atagatattg
    24001 ttttgtttca tcaatagatg tatgaaccct ccaagcatta tatttagctg aatccttgtc
    24061 gcttaaccaa ttataataaa tatcttgtgc atcggacatt tggaatttcc taagtacaaa
    24121 tcttcttgtt tcaatctgaa cagtccctga aagcattaat tttttcaaaa tgacctccgc
    24181 tgattgtact acttgtatta taccttttat ttataaaatt gcatagcttt atttttattt
    24241 gtgttgaatt ttattaataa aaaaagtata ataatctaaa aaggagtcgt atatggataa
    24301 taaagaaatt attaccgata attacagtga agccgtaaaa tcaaacactg agattaagga
    24361 taaaaagttt gtaccgacca ttgaatccgt tctaaaaaag aaacccatac agatgccgca
    24421 agaaatatac aatgccagcg gtataaaaat ttttgggaaa agaataaagt ccttaatcta
    24481 tacaactgat ttagcaatta taaagaataa taatgcagac ggggtaattg cagtatatcc
    24541 ttttactcct caattggcaa ttaaccaggc aattatagat atatctccta cgcctgtgtt
    24601 tgtaggtgtc ggaggaggta caacaacagg acagagatca attgatattg ctttaaatgc
    24661 cgaattgacg ggagcctatg gggtagtatt gaatgcgcct actaagaatg aactgatatc
    24721 cgaaatgaaa gagagattgg atataccgat tataattaca atagtgtccg aaaaagagga
    24781 tttcgaatca agaataaagg caggagtatc cattttcaac gtttcaggag gagcaaatac
    24841 aacaaatatt gtaagacaaa tacgagaaaa acacccatat tttccgatta ttgcaacggg
    24901 tgggccgaca cctgaaagta tattggaaac cataagagca ggtgcaaaca ttattactta
    24961 cactccgcct ccaaccggta aaattttctc cgaaatgatg gaggtataca ggcataaaat
    25021 cacagaagaa ttgagcacaa ataaataaaa tttaaagaac ctcctaattt aattatcggg
    25081 atggttcttt atttattatt acatcttttt aagtacttct ctattgtctt ctattatgtt
    25141 gtttatttct ttgattttgt cttgaagctt ggacaatatt tcatcgctgt aattatatgc
    25201 tccaactttc atttcgtttg cttttctttc tgcctcctca attatttcat ttgcataaat
    25261 tttagcttgt cttgttattt cactttcttc aattaaatgt ttctgttgct cttttacttt
    25321 attgataagt tcatctgcct cgctctgtgc ttcattaagt atacgctgtc tctcttttgc
    25381 aacccatgaa gctctgttga tttcatcagg taattttaat ctgatttcat ttattacttc
    25441 caaaacttct tctttgtcaa tcataacctt atttgataag ggaaacttag aagagtcttc
    25501 tataatatcc tctatccttt ccaataaatt caagatatcc atttttactt cctcccaagt
    25561 aatctatata tattttgtgc aacattatca ggaactaatc cttcaatgtt tcctccatgt
    25621 tcagccacat cttttaccat gctggaactt aaaaaagaat attccgatgt tgcaaccaaa
    25681 aatatagttt ctaattcatt atttaaatgt ttattaatac ttgatattgc cagctcatat
    25741 tcataatcgg atacggctct caaccccctt actataatgc tgatattatt ccttttacaa
    25801 taatctacta ctagccctcc cgtaaaatcc acctcaacat tttctaaatc cttaacggct
    25861 tctcttacca tctcacatct ttcttctatg gaaaaccagt atttcttctc cttattatta
    25921 aataccgtta ctacaacctc ttcaaatttt ttcgaaattc ttttaattat atcaaggtgt
    25981 cccattgtta tcgggtcgaa actcccggtg tataatactt tcattgttcc tccaaatatt
    26041 tgattatgct gattttagtc cttccatata ttttctcttt atattctatc atatttgcaa
    26101 cttcattatc aattactttt tcggatttgt cgctttcaat aataaggagt ccgtcgcttt
    26161 ttaatatacc atattcaaca attttattta atattttatt gcttatgttt aactcgtatg
    26221 gcgggtctgc aaaaatataa tcaaattttt cattgtcctt agataaattt attattgctt
    26281 tttcataatc agaggatata attttagctt tatcgctaaa attacataat tccaaattct
    26341 tttttacata atttaaactt ttacgagata tatcaacaaa tgtacagtgt tttgcacctc
    26401 ttgccaaaaa ttcgatgcct atgcttcctg agcctgcaaa caattctaat acagtcgact
    26461 cttcatcgat tgacccgatt atattaaaaa tcgcctcttt gattttatca ccggtaggtc
    26521 ttacactttt cccttcagga gcatacaatt ttttgccttt atttgttccg gatattattc
    26581 tcattagaac ctctttttat tttatttatc aattaaatac aattgtttcg tctttaaaca
    26641 aattcataat ttccattttt aaattttgat aatcagaacc ttttaaatat gggttttccg
    26701 ataaaattct ttttgataaa agttggactt cctgtactgt ttctacatta ttcactatat
    26761 tttgaatttc caattttgat aatccatgct gtcttactcc gaaaaagtct ccgggtcctc
    26821 ttatttttaa atcttcttct gctattataa acccgttgtc tgttcttttt aaaatattca
    26881 ttctatcttt tgatttttta ctcttacttt cattaaccaa aatacaatat gactggcatt
    26941 tacctcttcc gactctgcct cttagctgat gcagctgtgc aagaccaaac ctttcagcat
    27001 ttaaaataag cataacaact gcatttggaa catttacgcc aacttcgaca acggtggtag
    27061 agataagcac atttattttt cccctataaa atttatttat gatttcttct ttttcactat
    27121 tgctcatttt accgtgcaaa agacctaatt tgtaccctga aaaatgtgag gtgcttaatt
    27181 ctttatatat ttcaacagcg gaattcaaat ctaaattttc ggattcatca accaagggtg
    27241 ccacaatata tgcctgcctg ccgctgtcaa cttgactttt aataaaatca tatgcttttg
    27301 tcttttcctt ttcgcttatg atagaagtaa ttactttttg tcttcccgga ggcatttcgt
    27361 ctatcaatga aatatccaaa tcaccataaa tcatcaaagc caaggttctt ggaataggtg
    27421 tcgctgtcat aataagaata tgcgggtttt tccccttatt tgacagcctt gctctttgcc
    27481 ttaccccaaa gcgatgctgt tcgtcggtaa ccacaagccc tatattttta aactccacat
    27541 tttcttcgat aagagcatgg gttccaacta taatatcgat ttctccgcct ttgagtttat
    27601 caagaatttc ttgtttttct tttggctttg ttgaacctgt taaaatttct gccttaacat
    27661 ccaaatctgc catttttata aactctttaa ttgtatcata gtgctgcagg gctaatattt
    27721 cggttggagc catcatggat gactgatatc ccgagccggc tgccattagc attgcataaa
    27781 cagctaccac ggtctttccg gagccgacat ctccctgaac aagtctgttc attgtttttt
    27841 gacttttcaa atcttctttt atttcctcaa taactttaag ctgtgcctta gtaagcttgt
    27901 aaggtatgga gctcaatagt ttttctgaca gctctgtgtt tcttaataca atttcattta
    27961 attcattggt taaattattc tttatagtaa aaagaccaag ctgcattatc aataattttt
    28021 caaaggcaat agttctcttt gccaatgtat atttcttatt gtcgcttgga aaatgaagag
    28081 cttttactgc agcttttttt gaaataagcc catatttctt tattatttcg tcaggcaaga
    28141 cattttgaat ttcatcatag tatgtttcta aggcagtctt tactaacttt gtaatttcat
    28201 tatttgacaa tccgtatgta agaccgtaaa caggcattat ttttcctgcc ttccagtctt
    28261 tattaataaa ttcgatttca ggatttgtag attctaattt gccataggca atttttacat
    28321 ttccaaaaac atagtagtat tcgccaacaa tcagcttatc ctttaaaaag tcctgattga
    28381 aaaatgtaag ggtgataaaa cccgtatcat ccttacatat agctttaatt attgacatat
    28441 tctttttgat tcttgaatat ttgggttttt caaaaatcct gacttttaac agatatttac
    28501 cctgctcaca tatttcattt atatttttaa ctgttctcct gtcttcatat ttaacgggaa
    28561 aataatttaa taaatcgctc actgtaaata tgtctaattt gtgcaatttt aaagccctgt
    28621 tcggccctac tccctttaaa tattgaatat ctgcatctaa catttttcac cactacatgt
    28681 tatagaagtc tgcctgcctt gttttgttat tctaccgaga ttatatagta ataaagaggc
    28741 tgacctccgt atataatttc aatatcgcag tcttctgcaa tctcttcaat ccttggttta
    28801 atactttctg cagttttttc atcgacatta tctccgtaaa atacggtaac taaaaaactg
    28861 tcttcgtcaa ccattttatt tattaattca actgcttcgt cttccggatt ttctccgtga
    28921 tttgttatat cgccttcata aatagcaata atttggtctt ttttaatttc taaatcatta
    28981 aatacagtat cccttacagc ataagttaca agccctgttt ttacttcctt taccgcctgt
    29041 gacatggctt caatattctc gtctatatca ctgtcttctt taaatgcaat aagagctgct
    29101 ataccttggg gtatagtctt gcttggcacc acatataaat ttttgtccga aattttcttt
    29161 gcttgtgttg cagctaatat tatattggaa ttattgggta atattataat gtgctctgca
    29221 tttatgtcgt ttgctgcttc caatatatct tccgtgcttg gattcattgt ttgtccgccg
    29281 gacaaaaaca catctacatt taaatcagta aatacttttt ctattccctt tcctacgcct
    29341 atggaaacaa aaccgtattt cttttttttc tgattatttt tgtgattttc cttaagtctt
    29401 tccctaaatt gctccttcat gttgtcaatt ttaatgtttg aaagctcacc gaatttaact
    29461 gccatagcca atatttggcc tgggttgttg gtgtgtatgt gaatttttat tacattttca
    29521 ttgccggcgc aaactatgga atcacccttg tcataaattc ttgttaaaaa ttcctctgcc
    29581 gtctttatgg gatttttaat caaaaattca gtgcaatatg caaatataat ttcatcttcg
    29641 ctcgtaaatt catctaattc aacttctgta tcaatcacgg cctgctctgt tatctcctgt
    29701 ttgatttcta ctccttttag tgccgcaagt gcaccttcca taagatatat taatcctttt
    29761 ccgcctgcat ccacaacatc tgcctgtttt aaaacattaa gcatttcagg agtgcgattt
    29821 aaagtatctt ttgcttgctg aataagactt tctataaatt ctattacgtc ttcataatct
    29881 gtataagttg ccattgcttt ttctgccgtt tcccttgcaa ctgtaagcat tgttccctca
    29941 acgggcttca ttaccgcttt ataagcagta tctgaggctg atttaaggga ttctgctatt
    30001 attttgcaat ctatatgatt aagaccctta agtgcttttg acattcctct taacaattga
    30061 gacaatatta ctccggagtt tcctcttgct cccataagtg agccgttagc tgccgcactt
    30121 gcaagagatg taattgtgct ttctgaaaga ttgtttattt cctttatagc tgcctgcaaa
    30181 gtaagattca tattcgtacc ggtatctccg tcgggaacag gaaatacatt tagagaatcc
    30241 acaattgttt tattgttttc aaggttaacg gcagccccca aaagcatctt tttgaatttg
    30301 acattatcta tgtacttaat taacatttta gcctcctgat tacttttgca cccttacacc
    30361 ctgcacaaaa atattaacat tttcaacgct tactcccgaa aaagtttcaa cgctgtactt
    30421 aattctggaa attacatttt ctgctacaac ggatattttt actccatatt gcaatactat
    30481 atataaatct atatttattt tatcttcttc ggttgatgta acttttatcc ctttggttaa
    30541 ctgctctctt ttcagcagct caaaaaaacc ttgagttgca tttctggaag ccatgcccac
    30601 aacaccataa cactccattg cagctaatcc tgcaattgca gctaaggcct ggtcatcaac
    30661 atatatactt ccattttcgt taattatctt aactgacata ctaacctcct atttaatact
    30721 ttatttatca ttataacctt attaacatga atttacaatt ataatataat ttacttgatt
    30781 cctgaataaa tttctgcttc taaagtagca ctattcatta tactaattta aaattaatat
    30841 tgcaatattt aatttttaat gttagaatga tgaagttcaa ataagttacg ctcttacgaa
    30901 atattgcttc gctaaggata acactgtgtg tgtcagtaac ttatataata aataatgatg
    30961 tatacgttaa tataaatggg aggtggaatt atggcaaagt tttgtgaagt atgcggaaaa
    31021 ggaactatat caggacacag cataacgcat tccgatagaa aaataaacag aacatggaaa
    31081 ccgaacgtaa gaactatcac ggttatggaa gacggaagag ctttaagaaa aacagtttgt
    31141 acaagatgta ttagagcagg aaaaatacaa cgtgcaaatt aagcacatct atcattaaaa
    31201 tagtggccaa cgccattatt tttttgtgtc cccttccaaa ttggggacat tcgtcattcc
    31261 ctcggagact gtccatctat tagaggtgtg tccccatacc tataacagcc tgaataatgt
    31321 gcaacccaaa attatcaata atattcctaa cacaaacaac catactttaa caggaagcat
    31381 ttgaactata ataattgctc ctatggttgc aatcataagg cctaatattt cggtgaattt
    31441 aaaaaatgat ggtttatttg caggactgca tttcttttta tgcttaaagt aattcataat
    31501 atccccctta gaatttatgt agagctctac tattattaca ttctattcaa gcataaaaaa
    31561 aagatacaaa gaaattttct ttgtatctta cctatacctt aaaaatctgc atttttaggg
    31621 gttcttggga acggaattac atcccttatg ttgctcatgc ctgttaaata cataataagt
    31681 ctttcaaaac caagtccgaa acctgagtgt ttcacaccgc cgtacttcct taattccaag
    31741 taccaccaca tattatcttt tggaactccc atttcatcca ttcgcttttc aagaaattca
    31801 agtctttctt ctctttggct gccgcctatc aactcaccta ttccgggaac caataagtcc
    31861 attgctgcca ctgttttatt atcttcattc atccgcatat aaaatgcctt tatttcctta
    31921 ggataattaa ctgcaaatac aggcttttta aatatttttt ccgacaaata tctttcatgc
    31981 tcagtctgta tatctgttcc ccatttaacc ttatattcga aattatcatt gtttttcatc
    32041 aatatatcta ttgcttctgt gtatgatatt cttccaaact ctgaatttac cacattatca
    32101 agtttttcta aaagcccttt ttcaacaaag ttattgaaaa actccagttc ttctttggca
    32161 ttttccatga catagcttac gacatactta accatatctt ctgccaaatc catattatca
    32221 tttatgtcgg caaaagcaat ttcaggctct atcatccaaa attctgcggc atgccttgca
    32281 gtatttgaat tttcggctct gaatgtaggt ccaaaagtat aagttttctt aaatgccaaa
    32341 gcaaaaattt cagcttctaa ttgcccgctt accgtaagat ttgcagacct tccgaaaaag
    32401 tcctttgaat agtccgtttt tccgtcaacc ttttcaatat tatccaaatc cagtgtggta
    32461 actctgaaca tttcacctgc accttctgca tctgaagcag taattatcgg cggatgcgca
    32521 taaataaatc ccctttcggt aaaaaattta tgtatggcat aagcagctaa gctccttaca
    32581 cgaaaaactg cggaaaaagt gttggtgcgg ggtcttaaat gcgcaatagt ccttaaatat
    32641 tccattgaat gtcttttctt ttgcaaagga tagcttgaat ctgacggtgc ttccaaggct
    32701 atttctgttg ctttaatttc aaaaggctgc tttgcttcgg gagttaaaac tatgtttcct
    32761 cttaccgtaa tggcactgcc tgttgaaaat tttttaattt cttcaaaatt actcaagttg
    32821 ttttcaaaaa caacctgcac atttttaaga aatgttccgt catttaattc tatgaatcca
    32881 aaatttttag aatctcttat tgttcttatc cagccctcta tacggacttc ttcgtaattt
    32941 tccggcgata agaatatatc ttttatatcc gttgttttca tgaaccctcc tgttaatctt
    33001 tagatataat tactatagct cttccttttt ttaacgtaat ttttccctta ttatctttta
    33061 tttgattgct gatacaaaga gtcgagcctc tttcaacatc aacattgtca agattatatt
    33121 tgaatccttc caaacatagt ccctgaatac tatctgttat ggggattatc gatataaaat
    33181 aaccttcttt gtattctata aacagctccg aattatcgga aataattttc atttcattat
    33241 tgttatcaat tattgaaata ttaactccat ctttgaagta tttctccata agcaaaatgt
    33301 tgcccaaggt atggtctaat cttgacccgg aagctcccgt gagtattata tttttgtatc
    33361 ctttggaaac agccagttca acagctaatt ccatgtctgt aaaatccttt tcggaaggaa
    33421 atttttcaat aggtattctg ttttcatatt gtttcagaac attactagct accgaatcaa
    33481 aatcaccgat tattatatct ggaattattc cgtaatttga aactttttca agccctccat
    33541 ccgcacatat caaataatca tacttaatat tttcaattat tcttttggcg gtttcatctc
    33601 cgtttccgac aatcagcgct gttttcatga catgatttcc ttaaatttct ttgtttccaa
    33661 aacaatatcc tctgcatggt aaattgcaga gcctgcaact ataacatttg caccccagct
    33721 taaaatttcc tttaaatttg aataattcac gcctccgtca atttcaatat caatatctaa
    33781 attatattta tttaataatt gctttaaatc ataaaattta tacttgacat tttctatcag
    33841 actttgtcct ccaaaacccg ggtttacgct catcagcaaa accatatcca catctttaat
    33901 aatttcattt aaaacagata tgggtgttgc cggatttaat gatactcctg cttttaagcc
    33961 gtatgatttt attttctgga tagttctgtg aagatgtacg gttgcttcct gatgcacggt
    34021 aattatatct gctcctgctt tataaaagtg ctcgataaaa ttatccggat tctcaatcat
    34081 aagatgcaca tcaaatatga gagatgttgt ttttcttaaa tcttttacaa ctgccggccc
    34141 tattgtaatg ttgggaacgt aatgaccatc cattacatct atatgtatgt aatctgctcc
    34201 cccttcttcc acaagtttta cggcttctcc tagattagaa aagtcggcag acaaaattga
    34261 aggggctaat ttatttttca tacttcctcc ttaataaggc ttactttctt ttatttgctc
    34321 caaaagcttt atatagttat tataccttac tctgctgatt aaatcactat taacggcttc
    34381 ttttatcaca caaccgggct ctttgtaatg gaggcagtct aaaaatttac agccatcatc
    34441 atattttact atttctggat aataactttt taaagaatac tcatcaattc cttccaattc
    34501 gaaggaactg aaacccggcg tatcaaccgc atacccgcca aagcttaatt taaatatttc
    34561 cgcatgcctg gtcgtatgcc ttcctctttt taatttatcg ctgatttcac ctgtttccaa
    34621 ataaaatccg ggctctatgg cattcattaa ggatgatttt ccaactcctg aaggtccgga
    34681 aaacacattt aatctgtcct taaggatatc ttttaaattc tgtagggaat cctcatcata
    34741 cttgcttgta aaaataattt tgtagcctgt gtttttaaat atgttaagta tattttcttt
    34801 ttcttcttca tttgctaaat ctgatttatt aatacaaata acagggttta aattatttga
    34861 ctttgctgca atcaaaagtt tatccaacaa tataaagctt ggttccgggt tttttacgga
    34921 gaaaattatt acaagctgct gagcattggc aacgggaggt cttttaattt cgcttgttct
    34981 ctctttaatt tcttcgatat aacccatatc ctcatcttcc tctgtaatcc tgataaggac
    35041 tttatcccct accaaaggtt tgatattttt gagtctgaac aagcctcttg ctcggcattc
    35101 tattattttg ctgccgatat caacataata atttcctccg acaccctttg ttatgattcc
    35161 ctcaatcatt aaaactcctt tttattcaaa tgttatttca actctgtcat ataattcatt
    35221 gtcaatatat atttcgaata ttcctgttcc ttttccctta acattaacta ggatatttcc
    35281 ttccgaagta tccacttcct ctgagtacac aacttcacgt ccgtcactgg ctattctttg
    35341 aatcactatt gatacactat ccctgtctgc aggaagagat attgccaagg gaaatactcc
    35401 ttcttccggt gtaggctcgt caggttcttc cggcttgccc gaacttaccg taagccatat
    35461 tgatgttcct tgttcgactt cctgaccggc tgttatactt tgtctcataa ccaagccctt
    35521 ttctatttct tcgctgggct cttcatttac ctgcccaacc gcaagaccat gttgaacaat
    35581 cgaaagcttc gccgtttcta aattcaaatt taacacattt ggcattttca catatataat
    35641 cttcggacct atactcacca catagcttac tttatcattt tccctagccg gtgtattagg
    35701 taaaggatat tgatcaatta tctgtcctgc caaatatgtg tcagaatttt cttctgtcgt
    35761 ttctccttct gaaagccctg cattattgag aattcctccc gcctctatag catacttgcc
    35821 tattaaatta ggaacaacta tttctttttc tcccctgctt attaccactt ctatggggta
    35881 attttcttta accttagagc cctcatcaat actttgctcc attacataac cttcatcgta
    35941 ttcagaagaa tattctcttt ttgaaacttt gaataccaga cccaaatctt caatgagctt
    36001 cttggcttcc tcttcacttc ttcccaataa actcggcacc ggaacttccg gcacatacaa
    36061 aaatgactta aatagaaata ctccgccaat tactgctata accaatgcgc ttaaaattgc
    36121 cgctatagta atttttttat tgtttatgct cttttcaaca ttttcatctt cttcatttcc
    36181 gctaaagaag cttttaaatg ctttgtcgga attttcgtca attaaatcta tattaataac
    36241 attttcttct aaatcattaa gcgcaggaat ttcaatagtc ggtgaatcta ttatttccgt
    36301 ttcgtctttt acgggattat tatttccgtt caatgcatca agctttttca gcagttcatc
    36361 agtgttttgg aatctgaagc tttgatgttt ttcaagacat ttcattataa tatcttcata
    36421 attttttgat attttaacat ttttcaccct tttggaaggc agcatgggtt tttcctgaat
    36481 ttgtttcatg gcaaccgaaa tatggttatc tgcatcaaaa ggaagagtac ctgtcaccat
    36541 ttcatacatt acaattccca atgagtaaat atctgatttt tcatcaacat atcctcctct
    36601 tgcttgttca ggagaaaagt aatgcactga gccgataaca tttgaagtgt tgtttatggt
    36661 agaacttgtt gatgctcttg caattccgaa atcagtaacc tttgctgttc cgtcctcagt
    36721 aagaagtatg ttgtgaggct taatgtccct gtgtactata ttgtgtgagt gagcatgctt
    36781 taaagcttct gccacctgtc ttgaaatttt cacggcttcc ttttcactca gactgccttt
    36841 tctttttata tagtttttta aagtttcccc ttttacatat tccataacta tataatagat
    36901 atcgtcttca aatcctgtat cgtaaatatt tacaatatta ggatgagcga gacttgccgc
    36961 cgataaagat tcttgtttga agttattaac aaaatcagaa tcctgagtta attcatccct
    37021 taaaattttt atagctacaa agcggttcaa aacgttgcac ttagccttat atacatttga
    37081 cattcctccg ccgccgattt tttctattat ttcatatctg tttcctagtg ttctacctat
    37141 cataagtttc acctctaatt atattatcaa caataattat ggtaatatta tcagttccgc
    37201 cattatcgtt tgcttgttta atcaattttt gcacactttc tgatgtgccg tattctttta
    37261 tgatttccaa catattatca tcggatacat gagttgtaag accgtcggtg caaagaagca
    37321 gtatgtcatt ttttaataat tccaattcaa aaatatcagc atgtacatat tcttcactgc
    37381 ctaaagcccg agttattaca ttccttttag gatgtgtttt ggcttcttct tccgtaattc
    37441 tgccatcctt tatcaactca ttcacataag tatggtcagt tgtaatttgt ttaatttcat
    37501 tgttccttaa caaataagct ctgctgtcgc caatgtttcc aatgtaaatt atattattaa
    37561 gcttatctat tacagccatg gttacggttg ttcccatacc gcaacaatct tcattattta
    37621 atgacacttc tcttattttt tcattgctca gcttcacgct ttccataatg aatgatgggg
    37681 gctgaaaatt ttcatccttc atacattcaa cgaaaaagtt tcttatctct tcaatagcaa
    37741 tggagcttgc cacttcaccg gctttgtgcc ctcccattcc gtcagccaca gcatacagac
    37801 aataattatc aaattgctcg actattaaac tatcttcatt attttctcgc attaaaccct
    37861 tatttgtatc aaagaacaca tccatacagt acctcacatt tttaacaaat catttctgag
    37921 ctgaccacag gcagcgttaa tgtcactgcc caattctctt cttaccgttg cattaattcc
    37981 gttatccgtc aatatttttt taaatttcat aatatccaaa tcttcgcttg gttcaatatt
    38041 tgcttctttt acataattgc atggaattaa attaacattt gctaatttat ttttcaataa
    38101 tctgcttaag ttttccgcat cttcacggct gtcattaaaa ccctttatca ttatatattc
    38161 aaaggtaagt cttcgccctg ttgttttaac atagtaatca catgctttta atatttcggg
    38221 aagcttatat tttcttgcaa tgggcataat ttgctctctt ttttcctgtg tggaagcatg
    38281 aagagaaatt gccaatgtta tcggaatatt ttcatcagcc aacttataaa tatttggtac
    38341 tataccgctt gtggaaagag taatttttcg catggaaata ttttggcctc tttcatcatt
    38401 tataatattt agaaatctca ctatattatc gaagttttcg agaggctctc ccgagcccat
    38461 aataacaaca tttgatattt tttcatttat atcattttga atcaggtata tttgcttaag
    38521 catttctgca gcagtcagat ttctgataaa acccttttta gtggaagcac aaaaatgaca
    38581 acccatcttg catcccactt gtgatgaaat gcaagcggta ttgccgaatt tatattttaa
    38641 ataaacactt tctattatgt tgctatcatc taactttatt acatattttt ttgtttcatc
    38701 caattttgat tcaagcttta attcaattat tgcttttgta aattcatatt cttttttaag
    38761 cgctgccttg gttttttgag ataaattggt catgtcatcg aagctttcga cagtcttatt
    38821 atgtatccag tcgaatattt gatttgcttt aaatttaggc tcgcctattt taagcactgc
    38881 atcttttaat tcctcaaagc ttaaatcatc aatttttttc atattaccct tctttaatag
    38941 acattatgaa ttgaattttg ttgtcaatta tatcataacc ttttcaattt cgcaataaaa
    39001 aacccatcca taccgtgcag atgcggataa atttcaatat agccgtcttt ggctgtttta
    39061 aaatcttcat ttataatatc agaaaaatca gctaaaataa agttcttgtt ttttaaaaat
    39121 ctattaatta tttcaatatt ttcctctttg tttgtggtac aggtagaata aaccaattcg
    39181 cctgattgtt ttaaatactt tgatgcattc tcaagtattt tatattggat ttctgttata
    39241 tcttttaagt ctgtttcatt atattttatt tccggttttc gtcttattat accgagtccc
    39301 gaacacggaa catcagctat aacataatct gcttttccga ctaaggattc atctagtttt
    39361 cttgcatcat atttttcaac tttaacattt tttataccca atctttttat ttcattttct
    39421 attagtctta gctttgccgg gtatatatcc cttgataata ttttacccgt attattcatc
    39481 atttctgcag catttaatgt cttgccgccg ggagcagcgc aaacatctat tataaagctg
    39541 ttttctttag ggtttaaaat ttgtccggcc agcattgaac tttcgctttg aacagaaaaa
    39601 agtccatttt tatattcatc tgtcttatct atttcaaatg ggttttcaat aatcaatccc
    39661 ttatcggcat atttgcattt gtaagatttt ataccctttt cttcaaaaag ctttatcaac
    39721 tcatctcttt gaatcttcaa ggtattaact cttatttcca aaggtgcttc ggtattgttg
    39781 gctatcagta ccttatcaac atccttgcca aactgttttt cccatttaac tatcaactcc
    39841 tttggatatg aatatttaac acttaaataa tcatcccggg gaagctcatt catttttttg
    39901 ataagattat ccttgctgcg caatatattc ctaagtaccg cattaacaaa tttcgatgct
    39961 cttatattac cgttgtcctt aatatactga acgctttcgt tcacaacaat attttcatga
    40021 gacttatcca aaaagaatat ttgatatact gctaacctca gcacaagcaa gacatcggtt
    40081 tccattttct ttgtttttgt tttggatacc tgattaataa tccaatcaat aaatattaga
    40141 ttttcaacaa ctcccaaaac cgattttcgg tacaaggaaa ggaacctatt atcaacattt
    40201 ttcatatcat tatttatcac aatgtttgaa taggatttat ctttaaatat tttcttcagt
    40261 gtttcaattg cttgcgctct gtaacttttc ataattcctc ttttcatatg acaaggggac
    40321 gggggtactg tcatcttgcc aatttattct ttcaatcatc ttttatatta tatcaagaat
    40381 acacgcctta aggggacgag gtactactct atcttcgtac aatgcttaac ttttctacag
    40441 cctgattttc atcgggcaaa aagaatatat cttttccctt attgctctca tatataaaat
    40501 cttttaaact attgctttta tatacggaaa agtcacctat gattgcgacc ttaaaatgaa
    40561 agttaataca tttttgcaac acttctccgg ctaatttggt gcttaagtca aaaaaatctt
    40621 ctgctattgc agatttatcg attattatac gatcgcaatc tgtttcatat gaaactgttg
    40681 ccaataaatc caaggctgac tgaccatcct ttattaaaat ttcattactg taaattttag
    40741 caattcttac attatttatt tttattactt ttatctccat agctactcca taaaattctt
    40801 ctttttcttt ttttgcctta gagcactgct gattctaaaa tggaaaatga tgatttatca
    40861 caattaattt ccgccatagc tccatacggc agacatattt taggctcggt atgaccgaag
    40921 ttcatattat ataaaatagg taaatcctct ttcccgaatt ccttcataac tttttttata
    40981 gcttctttgt attccgcata atatgtttca tcctgcggtt taccccatat cattcctttt
    41041 attctatcca atataccatt tattccataa attcttaaac tgcattcaat aatccacgca
    41101 ggcggttttt cttcagaggt ctctaaaaac agtatactat catcaaaatc ctctaacttt
    41161 ggaaagactt cagtaccacg aagcgaatcg aatacttcaa tacagcctcc gattaatcgc
    41221 ccctttgtaa ttcccttccc ttgcaagagt tcatatccgc tgttatcttt aaatttcctt
    41281 tgaattaatt tgttttttcc ctcccaggga agatattcac tggtccactt tggtgaaggc
    41341 tcaatttctc ctattgcctc tgatttaaac aaagtttttt ctatatgttc aactgtatat
    41401 ggactcattg aaacattttc tgcaaaatca accaatagtg tcggtccata aatactcgat
    41461 atccccgctt tgtaacagaa aagatgcgtt gttgttgtat cggaatatcc cataaaaatc
    41521 tttggattat tactgattat atcaaaatca ataaatggaa gcattcgtat actatcaatt
    41581 ccgccgatac atgcaataat acccttaatg gaattatcct ggaacgcatt cattaaatct
    41641 tctgctcgct tctcaggatg attgtatatg tattctgttc ccgccaatgt atgcttcatt
    41701 tcaacaactt ccaagccgaa aacttgctca agccgctctt ttccttgttt ataccgccac
    41761 aataaatttt catcacctgc accgccccaa gacaagctta ctaatgctat tttatctcca
    41821 cggtttaacc tgttaggctt ttttaaaatt ttcatataca atttctccta cagaattttt
    41881 ttaagtattt aacttaataa ttcaaatttt cctcttacta attataacag ataatccgca
    41941 actattaata tacctgagca atacgatatc ttttcataat tacaataaaa ttcatttaga
    42001 ctcatcatca tttcatttgc aacaaaataa aattcttcat catcccaaat ttctgtatgc
    42061 gtttccttac attcttctaa ttctttcctt gtcatgaaag aaacatcacc aattaatatt
    42121 ttaccattaa catttaacag ttttaaaagt gatttaataa atttaatttt ttcttcatcc
    42181 ggtaaatgat gaattgcata tgtgcttatt atataatcgt atttattgtt tttaatttct
    42241 acaggtagcc ctctcgtaaa atcccaattt attaatatgg cctccggcat ttcgctttta
    42301 gctatatcaa tcatggtgcg tgaaaaatca attcctgtaa tatgataacc ttcattatat
    42361 agttgtgttg ttaatattcc ggttccaaat ccgatatcca aaacgcttga ataattatgt
    42421 ttcctaactt ggttataaat ataatttaat acatctttgt agccggcaaa tggatattca
    42481 ttgttttctt cactaaggtt tacactctta tcataaccat gtgcccataa gtcaaaacct
    42541 ttactgtcta acatactttt ctcctaattt tcaagtttcc ttaattttca atcattacaa
    42601 atggacatcc aatacatcac ctatatagac gcctgttatt aaattgtaat catcttgaag
    42661 tttttccatt gaatctccta caaactcata tctactttct gtagatatta ttccaaacac
    42721 cactccggca atgaaccttt tacagcccac catgacttta gtctcttgcc tgaaaatccc
    42781 aatcttctat aaatacttgc tgcatccggg ttgtctgtat gcaagtaaat caatgaatta
    42841 tgataatgtg ataaaacagc atatacgaca gccgttccat atccattgca cctctgtgac
    42901 ggtgttgtat aaacatctgt tatgtagaca acatcattat atttttcagc taatgccatg
    42961 cttacaggct tctttaattc attataagca aaaaacatat tgtttcctga agcaatttgt
    43021 ttgtttaaaa gctttacacc atatgtttca ccattatctt gttctattgc taaattatat
    43081 tcctgacctt caagagcacg gtttattatt tttacctcaa ctttttctgc aattttattt
    43141 ctggtactat gtgtatctaa aatcatcaaa tccatgtcaa attctcttat tgcatagtca
    43201 tgctttgtga aaaatggtct taatatatcc agttccaccg agccgcagcg attaaatatt
    43261 tttggaatta tgcttcttga attatagtat tcttctactt ctttaattat attgtcaaca
    43321 ttttcatttc tgtaaattac agcataatta gatgtatagc tttctttatt ccctatgtca
    43381 caaagcattc gtccccatga cgttaattta tagtctaaat acaacatggg gctgaatttc
    43441 tcaaattgag caattcgttc taaattatcc atatgtcttc ctcgcattat tttgcagtta
    43501 tattaatcac aaaatttata tttcaagctc ggtccccaga gttcttaatg tttatatcaa
    43561 atttttttgc atgacttcct tgtttaaacg gatttatttt cttgattgtt ccgtcatgtt
    43621 tgaaaagtga ccctgtgcct ttaaaccaaa atgtaatatc atatttctta tattgctcac
    43681 gaatatccaa tacccaatca taatcgcaaa gccgagcttc ccgtccactt tcatcattga
    43741 ctgtaacatg atgtatttca ttaagatatg aagacagatt aatatgatat tttttgtcct
    43801 tctatctttt attattcaat tattcaacag aaataaatac tgtttttaag aatttgattc
    43861 agaatccaca acactccata tgggtttact gttatcatca aagctgcata taaatatagt
    43921 tccgctcttt ttccccacag cataataact gcctccccac tcggctgtac ctttcacgcg
    43981 aaagacccaa catggttcat ctattgtata aattgaatcc agcaatgctt ctgcaaaaac
    44041 agcattctcg tctgtgcttt ccggccaatc gccaagaata taccagctta ctggttccaa
    44101 ctccattgta agctttgttt gccctatatc ctgtgcttct tcctccgtga tgccatcttg
    44161 tgaaggattg gcgcgcagga attcatacac atcattatat cgtatatcaa taccgctacc
    44221 gccactttcc caaatacttt caaggtttac catatccggc ttgccggttg aatctgaatg
    44281 tacaactaaa cgatatcctc cttctacttt ataaaccata atgtatcgtt catgacttga
    44341 actggtattt cctcccttat attgccataa atcttcaaac aataaatcat cgccttttcc
    44401 ggcaagctgg tgtacatcgt caagtgtcat ttctttcccg tcaatattgc cgccccatgg
    44461 gaacggaaca gcagttgttt ccgtagtggg tgtattttcg tcagtgataa tatcatccgt
    44521 ctcattgcta ttactattaa ccccatcaca tgcacttaat ataacgactg ccatcactgc
    44581 aatgacgatc atcaagggta gtttattaaa atttatcata gttaaagtca cctccttgat
    44641 taaagaaata ctcctgccca tcaagagtat ggcttatata aatactaata ctataacact
    44701 aaatagattt gcagcaaaat gcgatattgc actaacccaa gcattctttg atttgtaaaa
    44761 tataattcca tataaaatac tgtttataaa gacaaagaaa atatcatata ttacaatgac
    44821 aggatttccc tgattaaaat gtccaatggc aaataataga gatgaaatta gtagtacgtg
    44881 tataatagaa aaagtcttgt tcaactgctt ttgaaagaag gcacgccatg caatttcttc
    44941 tcctaaggca agaaaagcaa gttgaaatag cattaataaa accttatcaa aagataaaaa
    45001 atatcttatt gcctttgcga cttgttatcc ctgctttcat gttctgcctc cttaagcaac
    45061 aataatccgg taatggatta gattaaccg
//
LOCUS       DAOPUE010000005        44017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_791_length_44017_cov_9.755425, whole genome shotgun sequence.
ACCESSION   DAOPUE010000005 DAOPUE010000000
VERSION     DAOPUE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 44017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44017
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_44017_cov_9.755425"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1413)
                     /gene="purD"
                     /locus_tag="PKH42_01050"
     CDS             complement(160..1413)
                     /gene="purD"
                     /locus_tag="PKH42_01050"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOA19043.1"
                     /translation="MKILVVGSGAREHTICWKLKQSSKLSKLYCAPGNAGISKIAECV
                     DIKADELEKLVEYSVKNSIDLVVVGPEVPLVAGLVDMLNEKGIKAFGPVKAGAKFEGS
                     KSYSKDFMKKYSIPSANYEIYTDSEKAISELNKFNTPIVVKADGLAAGKGVLICETHE
                     EAKEAIKSIMVKKQFGEAGNSVVIEEFLKGTEASLLCFVDGKNMIPMESARDYKKAYD
                     QDKGLNTGGMGCFSPNTIFTEELNKYIKENILDRTLNGFLNENIEFKGVLFIGLMIEN
                     NQAKVLEYNARFGDPETEVVLPRLKSDLIDIMLKCIEGNLTEDDLQWEDNKCVTVILA
                     SGGYPESYEKGKEITGINEVDDDIIIFHGGTKLSGNKTVTDGGRVLAVTAMGKSLEEA
                     RKKVYKNIPKINFEKMEYRTDIAKI"
     gene            complement(1422..2981)
                     /gene="purH"
                     /locus_tag="PKH42_01055"
     CDS             complement(1422..2981)
                     /gene="purH"
                     /locus_tag="PKH42_01055"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOA19044.1"
                     /translation="MMRALISVSDKTGIVEFAKELERLGVEIISTGGTHKKLNEAGIK
                     VRGISEVTNFPECLDGRVKTLHPNIHAGLLAMRSNPEHMKQLLDLNIKPIDLVVVNLY
                     PFKQTVLKEGVSREEAIENIDIGGPTMLRSAAKNYQDVAVIVDPKDYELVLNELKENK
                     EVSLDTKFYLCSKVFSHTAHYDTLIASYIKRERKDNTLPDTYTMTFEKIQEMRYGENP
                     HQKAVFYKEVKNVDGLLASALQLHGKELSFNNINDTNGALELLKEFDEPCVVACKHAN
                     PCGVGCGDNIYEAYMKAFNADPTSIFGGIVVFNREIDERTAQEINKIFVEIIVAPSYS
                     QEALSVLMSKKNIRLLQIENILQKQDEYSYDIKKVAGGLLVQEIDSKLLPKGEELKTV
                     TSSKPTEKEMKDLFMAWKIVKHTKSNAIVIVKDGQSLGIGPGQVNRIWACKQAIEHAY
                     DFLGKDSTKGASLASDAFFPFPDCAEEAAKAGIRAIIQPGGSLKDDESIEVCNKYNIS
                     MVFTGMRHFKH"
     gene            complement(2983..3609)
                     /gene="purN"
                     /locus_tag="PKH42_01060"
     CDS             complement(2983..3609)
                     /gene="purN"
                     /locus_tag="PKH42_01060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOA19045.1"
                     /translation="MPQVKIGVLISGSGTNLQSLIDNIETGNIKGKITVVISNRKDAY
                     GLNRARQKNIDALYIRQKDYESFENFNDAIIDELKKHNVELVVLAGYLKILTSKFIEE
                     YRNRIINIHPSLIPAFCGKGYYGIKVHEEVIKYGVKLSGATVHFVDEEADTGPIIIQE
                     AVEVGYEDTAETLQQKVLKIEHKILPLAVKYYCEGKIQISGRKVKIKE"
     gene            complement(3597..4637)
                     /gene="purM"
                     /locus_tag="PKH42_01065"
     CDS             complement(3597..4637)
                     /gene="purM"
                     /locus_tag="PKH42_01065"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOA19046.1"
                     /translation="MTDKLTYKDSGVDVNSGYETVKLIKEHVRKTHIKGVISNIGGFG
                     GMFEIDKDEYEEPVLVSGTDGVGTKLMIAFMADKHDTIGEDAVAMCVNDVICQGAKPL
                     FFLDYIATGKLEPKKAASIVKGVSEGCIKAGCALIGGETAEMPGLYDVGEYDIAGFCV
                     GIVDKKKIIDGSKIIEGDILIGIPSSGIHSNGYSLVRKVFFSHKNYDITDYVEELGKT
                     LGEELLTPTRIYAKLVLDLISKFEIKGLSNITGGGFYENIPRMFPQGITADIDTKNIN
                     VLPIFKLLQKKGNIDIDEMYGTFNMGIGIVLTVAKDDADDILSYLKSVGETPVIIGRT
                     VKREGGLKLCHR"
     gene            complement(4647..6035)
                     /gene="purF"
                     /locus_tag="PKH42_01070"
     CDS             complement(4647..6035)
                     /gene="purF"
                     /locus_tag="PKH42_01070"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOA19047.1"
                     /translation="MFNLEEDKLHEECGVVGVYSDNPDITSQLIYYGLFALQHRGQES
                     AGIAIHTGKRIEFHKDMGLVADVLSQKVIDSLKGNIAIGHDRYSTSGESEKQNAQPLV
                     VRYKEGSIALAHNGNLVNADSIREILENEGVIFQTSTDSEVVANLISRHYRGSIIKSI
                     QTTMEVVKGAYAFVVMTDKELIGVRDNYGLRPLCLGKREDGYLLASESCGITAMGGEF
                     IRDIEPGEIVVINEEGVNSIKTTKYAQQRLCIFEHVYFARPDSNLDGVNVYETRYKSG
                     RLLAQEAPVKADLVFCVPDSGTPAALGYAEELSIPYGLGIIKNRYAKRTFIEPTDKLR
                     QQGVKTKLSILKEIVQDKVVVMVDDSIVRGTTTKQIVSMVREAGAKEVHVRIASPPVT
                     HSCFFGINTPDRKELLGANKTIEEIKDYIKADSVHFLSLENLIKSTGSNLGYCKACLN
                     GDYPMEVPNNGI"
     gene            complement(6092..6586)
                     /gene="purE"
                     /locus_tag="PKH42_01075"
     CDS             complement(6092..6586)
                     /gene="purE"
                     /locus_tag="PKH42_01075"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOA19048.1"
                     /translation="MKVAIVMGSDSDFPVVEKGYKVLKDFEVMADVRVISAHRTPYEA
                     LEFARNAYKNGYDVIIAAAGKAAHLPGVLAGCTTVPVIGLPIKSSELDGMDALLSIVQ
                     MPPGVPVATVGINAAENAALLAIQILSVKYEDLRNKFLDYKKQMAEKVFEKDKKLNEK
                     LMSS"
     gene            complement(6597..10322)
                     /locus_tag="PKH42_01080"
     CDS             complement(6597..10322)
                     /locus_tag="PKH42_01080"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOA19049.1"
                     /translation="MNFDVKRVFVEKRAGFNTEALNLLNNLKENLNIEKLNNLRILNR
                     YTVSNIDEDDFKKSVNTVFSEPNQDIVYFEDVELEGHVFAVEYLPGQYDQRADFAAQC
                     IQIVTGKEKPTVKYARVFVLEGDLSKDDIDRIKKYYINPVDSREAQLDKPHTLDDEYP
                     MAEDVQTINFINKSKQELEEFRNDIGLAMSFEDLAFCQEYFKNIEKRNPTITEIKVID
                     TYWSDHCRHTTFSTIIENIEFEKGKITEEIKNTYDEYLNVRKYVYEGKKDITLMDLAT
                     IGVKELRKAGLLDDLEISEEINACSIIINADIDGKNEEWLLMFKNETHNHPTEIEPFG
                     GAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRQKIEDTLPGKLPQRKITTEAAAGYS
                     SYGNQIGLATGFVNEIYHSDYVAKRMEVGAVIAAAPRENVKRETPKKGDLVILLGGRT
                     GRDGIGGATGSSMEHTEESILLRGSEVQKGNAPTERKIQRLFRKKEVSTLIKKCNDFG
                     AGGVSVAIGELADGLNINLDLVPKKYDGLDGTELAISESQERMALVVRKEDADKFNEY
                     ANEENLESTVVAEVTDDRRLKMTWRGKTILDISRDFLDTNGVKQKTNARIKEPLNSKY
                     KRSFKSNNIREIWLENLNDLNVASQKGMIERFDSTVGAGSVLVPLGGKTQLTPAEGMA
                     AKIPVEEGITNTCSLMTYGFDPYLSKESPYHGAMYAVIHSIAKIVAMGGDYKNIRLSF
                     QEYYEKLNKEPERWGKPLSALLGAFKVQHELKIPSIGGKDSMSGTFNELNVPPTLISF
                     AVTVSDVKNIISPEFKRANNKVVLIKIKKDENNIPDFEDIGIKYSAIHEAIKTGKVLS
                     AHTVGYGGIAEAISKMTLGNEIGFRSDFTIGNDWFDSSYGDIVLEVDNSFLPFADCIE
                     IGKTINEKEIWVNGICISLEEIKTNWLKPLNNIFPVKKDGAGEIENYSFNSKHIAKSS
                     ISIAKPRVFIPVFPGTNCEYETKRAFERAGAKADIFVFKNLSSQDIKDSITEMNSLIR
                     QSQIIAFPGGFSAGDEPEGSGKFIAAVFRNELLKDAVTDLLNNRDGLIIGICNGFQAL
                     IKLGLVPFGEITDMENDYPTLTYNKIGRHIATFVKTKVVSNLSPWLRMCSVGDTHYLP
                     VSHGEGGFAANQKWIEKLKTNGQIATQYVDLNNNPTYDGFYNPNGSVEAIEGITSPDG
                     RVFGKMAHSERLDENLYKNIPGNKNQPIFESGVKYFK"
     gene            complement(10579..>11479)
                     /gene="buk"
                     /locus_tag="PKH42_01085"
     CDS             complement(10579..>11479)
                     /gene="buk"
                     /locus_tag="PKH42_01085"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOA19050.1"
                     /translation="SYLKEKQFDIKELSCVVARGGMLLPVKSGAYVINDIMLDRVENR
                     PVMEHASNLAAPIAYGIAKEAGVPSYIYDSVMTDEFPDISRISGMPDIPRKSASHVLN
                     TRAMAIKYAKSIGKSYEELNTIVAHLGGGISINIHHKGQIIDLVSDDEGPFSPERAGR
                     VPCRELINLCYSGKFDKKTMQRKLRGSGGLKAYLNTVDAREVESMIKEGNEYAKLIYE
                     AMAYQIAKGIGELATVVEGKVDVIILTGGIAHSEMLTSWIKNRVSFISHVEIMPGENE
                     MEALAFGALRVLRGEEKAREYIE"
     assembly_gap    11480..11579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11580..11911)
                     /locus_tag="PKH42_01090"
     CDS             complement(<11580..11911)
                     /locus_tag="PKH42_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOA19051.1"
                     /translation="MKDTKNILLVGVGGQGTILVSKILSTGLMTAGYDVKMSEIHGMA
                     QRGGSVSTQVRYGSEVFSPIIGIGEADILVSFETMETLRWLEYLKPDGVVVINDFEIA
                     SAPILMGKA"
     gene            complement(11913..13688)
                     /gene="iorA"
                     /locus_tag="PKH42_01095"
     CDS             complement(11913..13688)
                     /gene="iorA"
                     /locus_tag="PKH42_01095"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOA19052.1"
                     /translation="MKVLMTGNEAVAQGAYEAGIHFAAAYPGTPSTEILENIALRKEI
                     TAEWAPNEKVAVESAFGASLAGARTLTAMKHVGLNVAADPFFTIGYTGVNGGMVLVSA
                     DDPGLHSSQNEQDNRYYARMAKVAMLEPSNSQECKDMVIKAMEISEKYDVLVMLRMTT
                     RVCHSKSIVETGERIEVPVKPYVKNAPKYIMAPANAKAAHVVVEKKLKELEKFSNETE
                     LNYIEWNDKKIGVISAGVAYQYAKEVFGKDASYLKLGFTFPLPMEKIKKFAEEVETLY
                     VVEELEPFMEEQIKAAGIKCIGKEKIPNIWELNPDIVKKSIFGTENEIIDYDRTIAVG
                     RPPTLCAGCPHRAFYYQLSKKKNTIVTGDIGCYTLGGAPPLNSMDTCVCMGASISMAH
                     GAKKAFERNNDDKRVVAVIGDSTFFHSGMTSLLDVVFNKGNTITCILDNRITAMTGHQ
                     DNPGTGYTLQGDSTDAVQIEPIVRALGVKNIKTVNPMNQAEMKAALNWAYDLNEPSVI
                     ISRYPCALKKYTKEDLEEFGPIQGYCEVDEETCIGCKICVGTGCPAIVFKKDIKKSSI
                     NKFQCVGCELCLQLCPTKAISKVGE"
     gene            complement(13720..>14187)
                     /locus_tag="PKH42_01100"
     CDS             complement(13720..>14187)
                     /locus_tag="PKH42_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HOA19053.1"
                     /translation="EDIVVNAEIYNVEGFSGHADKPALLNWIGSFKEKPGRIFIVHGE
                     KESKESFCIDVKEKFNIDCIIPEYDVIYEIKKKNLINEVVLVDEKQKIKDKYIQELLK
                     EVEELKKAFENALAKTEGCLLVDDIDIDKFRKINNNIKSLENEIMNLTMISAK"
     assembly_gap    14188..14287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14288..15249)
                     /locus_tag="PKH42_01105"
     CDS             complement(<14288..15249)
                     /locus_tag="PKH42_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA19054.1"
                     /translation="MKIRFLGAVNGVTGSNHLIQIRGKNIMLDCGMYQGKDEELNAEE
                     FAINPADIDCLLLSHSHIDHSGRIPLLVKKGFKGAIYCSKPTYELCEIMLLDSAHIQE
                     AEAQWKNNKAKRSGRKLVEPLYTQEDVLDSLQYFKPVLYDQIINIDENITVKFNDAGH
                     ILGSSIIEIWFKDEKDTIKLVYSGDIGMREKPILKDPAQIDRADYLIMEATYGNRVHE
                     NIEKRTEELINIILKTTKRGGSVIIPSFAVGRTQELIYELNKYYDSHLNEIGTKENEL
                     RKIPVYIDSPLAMKATEVFKRNSYAFDKEAKDYIMKGDNPLDFE"
     gene            complement(15622..16173)
                     /gene="spoVT"
                     /locus_tag="PKH42_01110"
     CDS             complement(15622..16173)
                     /gene="spoVT"
                     /locus_tag="PKH42_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HOA19055.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDREGEI
                     ILKKYSPIGELTEFAGEYVESLFETTRHIAIITDRDGVIAVSGSSKKDYAEKRLSPEL
                     EKIIESREIYQTSATSKPIRITANEFNPDNYTSQVIAPIMVHGDPIGAVILLSKDKGS
                     KMSEVEEKLIKTASIFLSRQMEN"
     gene            complement(16368..17309)
                     /locus_tag="PKH42_01115"
     CDS             complement(16368..17309)
                     /locus_tag="PKH42_01115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA19056.1"
                     /translation="MNKFKKSLLLITLLCIALVFTACSNSGGKDVDKEGVMATVNGKP
                     IMQKDYDDALAYYKDYVEYQLGEDAWETETPRGTYKEIYEEYVWDEMTKSLLLLDAAE
                     KEGIKASEEEVQHSLENFKINFENDEEYKSFMEQRGITEDFIIQELTKEILINNYITS
                     KIDSLNPNNEELKNLFEEMKLNVLIKASHILVDTEEEAVKIVERINKGEDFGELAKEL
                     SIDTASGANGGDLDYFKYTDMVKPFSEAAFALEIGEVSQPVQSDFGYHIIKLTDKIVD
                     EETTVESEKEKLIQYYKLNKYEDLLEQLKNDAVIVKN"
     gene            complement(17296..20820)
                     /gene="mfd"
                     /locus_tag="PKH42_01120"
     CDS             complement(17296..20820)
                     /gene="mfd"
                     /locus_tag="PKH42_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOA19057.1"
                     /translation="MNRILFDQFQRTEKFKKICSNYNKEKSQLIQGLNEEGLAYLACN
                     LLDTSDKILILTSSEAKSKKYEEIIRAYTEHSERLQPKEFILYNVDALSKDVEYKRAN
                     LLNNILNKRKCIVTASINSIITRVMSKDRFKESIIELKYGDSYDLNELRSNLVKLKYE
                     RVDAIEGVGQFSVRGGIIDIFSPSEANPFRVEFFDDEVDSIRLIDLKTQRSVKNIKSA
                     KILPCSDILFKKEEIKIILSEIEKDYDKQSQNQSLSKEAFKNLKNLYKSYQDKLMGGM
                     GIENADLLIPFIKESFSSIIDYFEDDSILLLDEPERIFEELNDLNESFQLKYTELFER
                     GEIFSNQSEVYISEHELLTKISKKPFISINGKEKIIKTDDSVHMLFKEAPSYYGKIEE
                     LSKDLNRLKYKGYKVDIILSTYESCSKLYNLLNDYECTTTLSKDTNAAESGQVVIAPG
                     ELTKGFEYYDNKLLVLTENEVLGSVKRKSRKTKKRKGAAIEIFTDLKVGDYVVHEHHG
                     IGQYIGIEKIEVQNIQKDYLCIKYKGEDKLYVPVDQMSLIQKYIGTDSEKPKLNKMGS
                     SDWIKTKERSKAAIENMASELIKLYAERKVLKGYAFSKDTEWQKEFEYKFPFEETEDQ
                     LRCVKEIKKDMEKPICMDRLLCGDVGFGKTEVAMRAAFKAVMDSKQVAMLVPTTILAQ
                     QHYTNLVERFRGYPIKIEMLSRFRTHYQQNKIINDLNKGLIDIVIGTHKLLSKELKFK
                     DLGLLIIDEEQRFGVRHKELIKQLKSNIDVLTLSATPIPRTLHMSMIGVRDMSIISEP
                     PGDRLPIQTYVIEYNEGLIKDAIEKEISRKGQVYYVHNRVADIDQTAAKLRKLLPEAR
                     IAVAHGQMSERHLENIMLEFVNKEYDILVCTTIIETGMDIPNVNTLIIDNADYLGLSQ
                     LYQLRGRIGRSNKVAFAYLTYEKDKMLSEVADKRLKAIKEFTEFGSGFKIAMRDLEIR
                     GSGNILGPEQHGHMLAIGYDLYVKFLDRAVKEIQGKQVEEDIETSVDLNVDGYIPSSF
                     IENEEQKIEIYKKIAAAENKDDIFDITEEIIDRFGNIPVQVDNLLKISYIKSLSKRIK
                     IKSIAQAGNTVNIEMTKDYLSQDIIEFLIKNYYEKISFVGTNEPIIKYKLDSLEQMKI
                     LRELEEFLETLNNFKAGKTAVEEKHEQI"
     gene            complement(20878..21447)
                     /gene="pth"
                     /locus_tag="PKH42_01125"
     CDS             complement(20878..21447)
                     /gene="pth"
                     /locus_tag="PKH42_01125"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOA19058.1"
                     /translation="MYVIAGLGNPGKEYASSRHNAGYMAVEYLAGKLNTKLNKLKFNS
                     VYGDTSINGEKVMLVKPVTYMNKSGIAIAEIVKFYKISTSNLIVIYDDIDIPLGALRI
                     RPSGSAGTHNGMKSIVDSIGSEFPRIRIGIGRNEEMDLADFVLQKFSRNEKDIVTPII
                     ERAAEAAVEIIENGIDSAMQKFNMKGDTL"
     gene            complement(21458..22828)
                     /gene="glmU"
                     /locus_tag="PKH42_01130"
     CDS             complement(21458..22828)
                     /gene="glmU"
                     /locus_tag="PKH42_01130"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOA19059.1"
                     /translation="MSMSIILAAGEGTRMKSDLPKTLHKVCGKEMIKYIIEASLNSGI
                     EKNIVVLGHGKEKVGHNIKDMNVQTVEQPIGEGHPYGTGYAVMAAENHIDDGETVLIL
                     CADGPLVKKETLIDFMNYHFENNCAASIMTAYLDNPRGYGRIVRKSDMSIDEIVEEKD
                     ASEEIKNIKEINTGIYCFKGELLKYALKKITKNNSQNEYYLTDTIKVLNNEGANIGGW
                     LLEDSDDIKAVNDRVQLAEVTKIMQKRINQRHMYSGVTIIDPDSTYIDDSVKIGRDTV
                     IYPNTYLEGGTVIGSNCTIGPNSRIIDSTVRNNVTVESSKVIESYIDDGTNVGPFAYL
                     RPGSNLGKNVKIGDFVEVKKSIIGDNSKSSHLAYIGDAEVGKNVNIGCGVVFVNYDGK
                     KKHKTIVGDGAFVGSNSNLVAPVTIEKGGYVAAGSTITEDVKEGDLAIARARQVNKEG
                     RGKNRF"
     gene            complement(23039..23332)
                     /gene="spoVG"
                     /locus_tag="PKH42_01135"
     CDS             complement(23039..23332)
                     /gene="spoVG"
                     /locus_tag="PKH42_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOA19060.1"
                     /translation="MKISDVRVRKINSEGKMKAIVSVTFDDCFVVHDIKIIEGQEGLF
                     IAMPSRKMPDGEFKDIAHPINSETRNLVAEAVFKAYEEKRTEESENVEEAALM"
     gene            complement(23430..24251)
                     /gene="purR"
                     /locus_tag="PKH42_01140"
     CDS             complement(23430..24251)
                     /gene="purR"
                     /locus_tag="PKH42_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HOA19061.1"
                     /translation="MKKYKRNQRIGALMKIFAEKPNCLFSYNYFCEIFNAAKSTISED
                     VAIVKELAAEMNFGRIETISGVSGGVMFIPILGKKELNEILEQLCSLFSQKNRILPGG
                     FIYMTDIFNNPRIVSNIAEVISSQFSNKHIDYIVTVETKGIPVAIMTGQKLNVPVAVI
                     RRNNKITEGATVSINYVSGSSNNIQTMSLSKRSLKENAEILIVDDFMKGGGTCKGMED
                     MMREFNANVVGTAVVIETIEPEKKLVDNYTSLLILNNKSIKEGVIEIKPNPKLLK"
     gene            complement(24311..25006)
                     /locus_tag="PKH42_01145"
     CDS             complement(24311..25006)
                     /locus_tag="PKH42_01145"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOA19062.1"
                     /translation="MKIIGIIGGMGPLATVHLFERIVLRTKAEKDQDHIRILIDNNTK
                     IPDRTLAILGNEESPVNELVNSAKMLENSGADFLIMPCNTAHYFIEEIKKNISIPFFN
                     MPEEAVKHTYDKYAKDAVVGIMATDGTIKTKIYEKYYAELGIKTVVPIKTQEKIMEFI
                     YDVIKKGNYDAGTSLFFECADELKELGANVFLLGCTELSSAQYIYKLEGNFINPMEIL
                     AETSIIFAGGQLN"
     gene            complement(25118..25798)
                     /locus_tag="PKH42_01150"
     CDS             complement(25118..25798)
                     /locus_tag="PKH42_01150"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOA19063.1"
                     /translation="MFDIKKYYNTYPYTGYMGYYAMMASCFCKKSYVRVLNTVNQPLD
                     IQVNDILMAENLKIGEFTKYVKFPPNSYHVLIHSPNGNLLFESDIDIDYNLAYTAVVS
                     ADEENKKDICILMVPEQKAHNMTSNMSALRLANLCIDEPEVVMSASDGTILFSDIKYG
                     KVSCNVAIPSGRYTLQLKKPDGTEILDHWIDAAPYMHYTLFIVGMKNDGSIKIIVPED
                     GVNYLDLC"
     gene            complement(25920..28331)
                     /gene="leuS"
                     /locus_tag="PKH42_01155"
     CDS             complement(25920..28331)
                     /gene="leuS"
                     /locus_tag="PKH42_01155"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOA19064.1"
                     /translation="MKSYNPQAIEKKWQEIWRKNETFKAKDNTDQKKFYGLVEFPYPS
                     GVGLHVGHIRAYTSLEVISRKRRLEGYNVLFPIGWDAFGLPTENYAMQTGKHPRKVTD
                     DNIKIFTAQLNAAGYSFDWSRSVDTTDPEYYKWTQWIFVQLFKHGLAYKDKTYVNFCN
                     SCKVVLANEDSQNGICDRCGSQVVQMEKDVWFLKIRDYADKLLEGLDEVDYLPRIRIE
                     QENWIGRSYGAEVDFKLKDTEDNLRVFTTRPDTLYGVTFMVMAPEHPLIKKYENIIQN
                     LDEIKEYQEESKKKTEFERVQLAKGKTGVEIKGLTALNPVNGEEVPIWVADYVMMGYG
                     TGAIMGVPGHDQRDWEFAKKYNIPIIEVIGGGNIEEEAFTDVEDGILVNSGMINGLRV
                     KDAIEKITNYLVENNLGERKVNYKMKDWAFNRQRYWGEPIPIIHCPHCGMVPVPEEEL
                     PLLLPEVENYRPAETGESPLANIPEWVNVKCPVCDEDAKRETDTMPQWAGSSWYFLRY
                     IDPHNKEQIASKEKLEYWGPIDWYNGGMEHVTRHMIYSRFWHRFLYDIGVVPFKEPYA
                     KRTAQGLILGADGEKMSKSRGNVVNPNDIIDEYGADTLRTYIMFIGDYEKYAIWNDAS
                     VKGCKRFLDRVWKMQEFVNEEKGYSHKHLTLMHKTIKKVSEDFEAMKYNTAIAAMMSF
                     INEIYNDGSITRDELKTFITLLYPVAPHITEEIWQEQGFEGMLNQTEWPVWDEEKTVE
                     DVIELPVQVSGKVRGKILLPKDADVNTARKLAEADENIFKYIEGKTTIKEIYVPGKIY
                     NIVVK"
     misc_binding    complement(28402..28598)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28715..29020)
                     /locus_tag="PKH42_01160"
                     /pseudo
     CDS             complement(28715..29020)
                     /locus_tag="PKH42_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
     gene            complement(29092..29883)
                     /locus_tag="PKH42_01165"
     CDS             complement(29092..29883)
                     /locus_tag="PKH42_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA19065.1"
                     /translation="MDKQTIARLAAEFVQKEINYVSNDKALNEELAGLKIFDEPIFAY
                     GAADDEGFQLLKSPSVIGEHFMMPKEWLQSANTVIVYFFPFTDEIREGNRTDMSWPSN
                     EWLHGRIDGQIFMNEFNLFLNSKLNEEGYKSLVPGLDSRFKSEGFSSNWSERHVAFIC
                     GLGTFGLSKGIITQKGMAGRLGSIVTELKLKSDIKKYNNIYEYCINCGICAENCPAGA
                     ISLENGKDHKKCSDFLDKVKEKHKPYYGCGKCQVCVPCENENPKD"
     gene            30093..30803
                     /locus_tag="PKH42_01170"
     CDS             30093..30803
                     /locus_tag="PKH42_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOA19066.1"
                     /translation="MITYNKHIEYLHELKRKIMLHRHRLTIIMMLVILLGTMASGCAI
                     GETDTTSPNTPAGESIVVTITDPPDELEPIVVPTMPEVIPPYLGADPETGLTMTGTPT
                     LVDFDTYRLKVSGKVDQELSLTYDDLRRMPKLTATPTLECVGYFKDVATWSGASLKTI
                     LEMAKVQPGSTRVAFKGADGYEISMILENALAPENFLAYELEGKTLPVLQGFPLRVVI
                     PSYDGSVWVKWLLEIVVE"
     gene            30836..31240
                     /locus_tag="PKH42_01175"
     CDS             30836..31240
                     /locus_tag="PKH42_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOA19067.1"
                     /translation="MKRLLIILFIVITLTITITGCTSMEKNQTGTETAYKKISAEEAK
                     AIIDSEDVIILDVRTQEEYNEGHIENSVLLPVNDITGKAGEILADKDAKILVYCRTGN
                     RSATAAKQLIKMGYTNVYDFGGINSWPYGTVK"
     gene            complement(31409..33928)
                     /locus_tag="PKH42_01180"
     CDS             complement(31409..33928)
                     /locus_tag="PKH42_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19068.1"
                     /translation="MKKGTEFFQRSISLIVLFGVLICLPASALSFIPTFETESINFDG
                     TQSSWAEAELKEAYENGLTYGQIENNYQKPITREEFCVIVVKLYEKLGGKAPVMGTNP
                     FTDTDNAEILKAYALGIVKGTSDTTFTPANNITRQEICVMILRALKAAKPGIDTSLGS
                     DFPFQDSSSIASWALDAMKFAYKNDIMKGTSTTTISPLNNTNREQAIILLKRTFTKYS
                     EAPEAQGQADPDEYYSSLLNGRAPSLNKNVFKPFVPANDSGSSSYSQFTAGTALSSGI
                     VISPTGPGLDITDSLKEKPGSDLRLLTPEEIEKYWDEYYDFPDESDYEPSVDWVKASD
                     NVLGVDCLGKGYDVIKGKFADPYSLKDSVLSTNKLLEAKRVFKTDGRQSVSRFTEGKS
                     LYSYSKEMARKIGVSGGYLYFSGSINASFGSQTYTETNSRYATLVSDLSQYLIEFDDN
                     YLDYSKYYDSSFKKDIEDDSVTPEEIFNRYGTHVIRAVKMGGRLDYNVQANSSYHSGS
                     HTFESEVKASFNMAFASAGASHSTSQKETTTSFNENCEINIKAYPDYLGSNTITPSQY
                     TAWGEYVRKYPAMCDFGEDTPLIPIYNLARNPFRRYELKKAYEKYAEENQYIPTAAIT
                     CINGIRLNPQPVGRTLDGLDEQVIIDPITNDKWYLVANITAHDFYRDEIQQQIYIRTG
                     FSDLASRPPVVAVFLVNESQGENAKEIFKKYWGNDPTAKLWGDGGEAKSDPSLKNEIS
                     SYADRLKLYYVTSTKGRPITALRVKHFGYDNLIRYYPPKDTNSPHDDYGKFFSVIDYG
                     THMKQGINENQNCAEGAVPLPFGPAHQNIYLQYSHDYSISY"
     gene            complement(34065..35318)
                     /locus_tag="PKH42_01185"
     CDS             complement(34065..35318)
                     /locus_tag="PKH42_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOA19069.1"
                     /translation="MVLDSDRVWKIVILIICLVLSALFSASETALMSLSKIRVKQMIE
                     NREKGANRINKLLSDPSRLLSAILIGNNVVNIGASSLMTSLAIDAFGNTGVGVATGIM
                     TLLILVFGEITPKSLAAKNSEKISVRLSGFIEFVTNLLTPISFVLNIITDFLVKLLGG
                     EVDKKKPFITQEELKTIVNVSYKEGVLEGEEKDMIYNVFDFSDSQVNDVMVPRTEIVA
                     IDVDLPYEEIIKIINKEQYSRIPVYENTIDNIIGILYVKDLLFLDVNKESPFDLRKYI
                     RQPYFTPEYKSIKELFKEMRTNRNHMVVIIDEYGGTEGIVTIEDVVEEIVGDIEDEYD
                     KKIKEIEVIKEDEYLVNGNVRIDIINELIGTHIESEDFDTIAGFVIGIIDRLPEAGEE
                     IEYENIRFIIENIDRNRIKKIRILT"
     gene            complement(35628..35981)
                     /locus_tag="PKH42_01190"
     CDS             complement(35628..35981)
                     /locus_tag="PKH42_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA19070.1"
                     /translation="MKIAVASEKEIVTEHFGHCENFNIYETDGNKIVKSESVKNPGHR
                     PGFLPNFLNDIGVNVIISGGMGQGAIDIFNEKGIEVITGALGEAKIAAEEYLRGNLKS
                     TGYICHEHQHHGECE"
     gene            complement(35983..36819)
                     /locus_tag="PKH42_01195"
     CDS             complement(35983..36819)
                     /locus_tag="PKH42_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOA19071.1"
                     /translation="MSENCNESCSSCSKECDSRKEEQTNFLERPHPQSNIKKVIGVIS
                     GKGGVGKSLVTSLLAVTMKRRNHNVAILDADITGPSIPKLFGITEKARGGSNGVLPVK
                     SKTSIEVMSINLLLENTTDPVVWRGPIIAGTVKQFWTDVIWKDIDFMFIDMPPGTGDV
                     PLTVFQSIPVDGIIVLTSPQELVSMIVEKAVKMAESMNIPIIGIVENMSYFKCPDCNK
                     EYKIFGDSKIEDVAKKHGLNVIAKLPIDPKISSMCDKGLIELYGDEPFTGIAEFLEEM
                     EG"
     gene            complement(36829..37680)
                     /locus_tag="PKH42_01200"
     CDS             complement(36829..37680)
                     /locus_tag="PKH42_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19072.1"
                     /translation="MKQLLILSGKGGTGKTTVAGAIIKLSNVKAYADCDIDAPNLHLI
                     MQQESIPENSDYYGLDKAVIDKDKCLMCNLCYDSCRFDAISDYTVSLYDCEGCGVCEA
                     VCPASAINLMPYKAGDMTLYRGNQVFSTAELKMGSGNSGMLVTEVKKNMRFAAADADF
                     AIIDGSPGIGCPVIASISGVTMVLVVAEPSLSGISDMKRIIETAKKFNTKLAVCVNKY
                     DTNIANNEKIKRFCQDNKIDFVGEIPFDKKAVEAVNNGLTIVDIDCAAGEAVRKIYKK
                     TMKILFN"
     gene            complement(37677..38510)
                     /locus_tag="PKH42_01205"
     CDS             complement(37677..38510)
                     /locus_tag="PKH42_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19073.1"
                     /translation="MMIAVLSGKGGTGKTFVSVNLSFVADNSNYIDCDVEEPNGHLFF
                     KPNIIKSEKVHVKIPNINNELCSGCRRCVDFCKFNALAYIKDKVHVFDEICHSCGGCM
                     LLCPEKAISEKDKEIGEIQEGVFNNSNIYTGILNPGEETGVSIIKKLLKITDNKPYTF
                     IDCPPGSSCSVMESIKDVDYCILVAEPTLFGVHNFNMVYNLVKLFDKPYGVVLNKCLD
                     GENPAEKYCNEKNIKVLCKIPFEHELGNINSNAEIASEKNEKYKSLFSSLLKTIKEEV
                     K"
     gene            complement(38510..38863)
                     /locus_tag="PKH42_01210"
     CDS             complement(38510..38863)
                     /locus_tag="PKH42_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA19074.1"
                     /translation="MKIAIPVDDGNVCVSFGRTPYFMLYDTENNDSSFINNSAANSQG
                     GAGIKAAQIIVDNDADILITPRCGQNAAEVLTAANVKIYKALNDSIEENLTAFKNDKL
                     KILTEIHSGFHGGEK"
     gene            complement(38932..39297)
                     /locus_tag="PKH42_01215"
     CDS             complement(38932..39297)
                     /locus_tag="PKH42_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOA19075.1"
                     /translation="MKIAIPTEEKSIKSNIYDYLGRAPYFLIYSTVTKEYEFLDNRAV
                     VEQGGAGIRAAQVIADNGVKAVITNRCGENAEKLFRNAEVFIYAAQKGTAEENIAYFN
                     ENKLSLLNEFHSSFHGKEK"
     assembly_gap    39384..39483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(39737..41287)
                     /locus_tag="PKH42_01220"
     CDS             complement(39737..41287)
                     /locus_tag="PKH42_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOA19076.1"
                     /translation="MTYSPALGSTISNTKMRTPENVSPYSGMCSVCTANCIGTCEIGL
                     SAVRGSEATFPYRRDINQFASEKDYPLDFSHLSINGRVFGALGCEEDANKATYTNVKT
                     ETEFGIKNKVKMKMPIILPAIAKLNWKDYFVGAALAGVSVVIGEDAIPNDKNLVLENG
                     KVVSAPLVGEMVDMFRKYSRGYGEIIMQANYDDENSGVLDYVIPKLGVKSVELKFGQA
                     AKGIQGMGRTNSLEEALKLQNKGYLVYPDPSDEKVAENFKNGKGPIFEKIGKLPIWNE
                     EILKNRIAHLRELGAERICFKTGPFDPKDLIRILKIASENEVDLVTFDGAGGGTGNSP
                     VKMMNEWGMPTVYLESMLYDILKRMDKKGYFLPQVAITGGFTLEDHIFKGLALGAPYI
                     QFVAVGRAAMAAAMVGKQVGELIEAGNIPKEYQSFGSTKEEIFADIRELKLYYDNAQD
                     LPTGAVGVYSYISRIAAGIKQLMALNRKFDIKYIDRSDIFPLTDLAAQVTGLDTYEDV
                     YVRELEKL"
     gene            complement(41429..43156)
                     /locus_tag="PKH42_01225"
     CDS             complement(41429..43156)
                     /locus_tag="PKH42_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOA19077.1"
                     /translation="MVYLLIAFGVLILIEGLIIYKLIMKNNKRLFSQPINAEKIINKE
                     NDLSGKYLELISRFSFSVEEIYMEMKEISGKVENIISESEEQTASMISISEVIENIYN
                     NVEINLAGSQTSSEISKKTHEEIYKKIMQLQDNIDELSRVRSLLDNISNLIADLETKT
                     LNAESLISRINNISQQTNLLAINASIEASRAGEYGKGFSVIAVEIRSLAAETNEVSNV
                     ISEIIKELRSSFVNTKKELIDVLDKIEEQTISIDKTIVSFKNIENISKELYNKTSDIS
                     FNSQKIVEQIYNLKEFINSTTLSVEEVTKSIVDVNQSVLLETKSVDNLNSSISKFEEV
                     NFEFLQLVKDYYPDNRIIIATMPYEPFIIYDIEKNIVSGIDIDMINEIYSEAGYQVEY
                     KIVPWETSINMAKKGLVDILPTIEINEEREEFLDFSINYRNESRYNFYTSKENNISIG
                     NIDDLSGKRVGVLTGYKYYAEFDQNTSFDRNYSFKEEIMFQKLLKGQIDVVILNSYSG
                     NYLFNKLNLVDRVKEEKYKYAHPDTSESRIGFVKAKNNKHLLDLFNKRFKELEKDGTI
                     KKITEKYLT"
     gene            43461..>44017
                     /locus_tag="PKH42_01230"
     CDS             43461..>44017
                     /locus_tag="PKH42_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOA19078.1"
                     /translation="MSSITLIFTSLGLSMDACAVSISNGMCYGNIHKKQIITTAAAFG
                     FFQAFMPVLGFFIGSAFSNAISFLDHWIALVLLGFIGVKMIVEAFRELKYPEVCITGA
                     KHLTSKTLFFQALATSIDALAVGIGFAVMKVNIFSAALFIGIITFVNSIIGSNLGKKC
                     GQLFKQKAEILGGVILVGIGIKIFL"
BASE COUNT        12195 a         9155 c         6323 g        16044 t
ORIGIN      
        1 aggctataaa ctcctatgcc ataaatatat tttcattttt ttataaattt aattttggca
       61 aactagaaat ggttatttaa agggtaagac aaactgcgga tcttgcgcag tctgacgggg
      121 gtattgtcat ctgttttatg cgatgttttg ttcataattt tagattttag caatgtcggt
      181 tctatattcc attttttcaa aattaatttt gggaatgttt ttatatactt tttttcgcgc
      241 ttcctctaag gattttccca tggctgttac tgccaaaact cttcctccgt ctgtaactgt
      301 tttattccct gataacttgg tccctccatg gaaaattatt atatcatcat caacctcgtt
      361 tattcctgtt atttccttgc ctttttcata gctttcggga taccctcccg aggctaatat
      421 tacggttaca catttattat cttcccactg caaatcatct tctgttaaat tgccctcaat
      481 acatttaagc attatatcaa ttaaatcgga cttaagtctt ggaagaacta cttctgtttc
      541 agggtctcca aaacgagcgt tgtattctaa taccttagct tgattgtttt caatcatgag
      601 acctataaac aaaactcctt taaattcaat attttcattc agaaaaccat tcagagtcct
      661 gtctaatata ttttctttaa tgtatttatt taattcttcg gtaaatatgg tgtttggaga
      721 aaagcatccc attcctcccg tattgagccc tttgtcctgg tcatatgctt ttttgtaatc
      781 tcttgcactt tccatgggaa tcatattttt gccatcaaca aagcatagta gcgaagcttc
      841 cgtacctttt aagaattctt ctataacaac tgaatttcct gcttcgccga actgcttttt
      901 aaccattata cttttaatgg cttctttggc ttcttcatgt gtttcacata ttaaaacacc
      961 tttacctgca gctaaaccgt cagcctttac aacaatcgga gtattaaatt tgttaagttc
     1021 actgattgct ttttcactat ccgtataaat ttcataatta gcagatggaa ttgaatattt
     1081 tttcatgaaa tcctttgaat aggatttgct cccttcaaat tttgctccgg cttttacagg
     1141 tccgaatgct tttattcctt tctcattaag catgtcgacc aaccctgcta ccaatggcac
     1201 ttcagggccg acaactacca aatcgatact gttttttacc gaatattcaa ctaacttctc
     1261 cagttcatct gctttaatat caacacattc tgcaatttta gatatgcctg catttccggg
     1321 agcacagtat aacttggaca gtttgctgct ttgttttaat ttccagcaaa ttgtatgctc
     1381 tcttgctccg cttccaacta caagaatttt cataagacct cctaatgttt aaagtgtctc
     1441 ataccggtga aaaccatgga tatgttgtat ttgttgcaca cttctattga ttcatcatcc
     1501 tttaatgagc cgcccggctg aataatagct cttattccgg cctttgccgc ttcctccgca
     1561 caatcgggga acgggaaaaa tgcatccgat gcaagggatg ctcccttggt gctgtctttt
     1621 cccaaaaaat catacgcatg ctcaattgcc tgcttgcatg cccatatgcg gtttacctgt
     1681 cccggcccga ttccaaggga ctgtccgtct ttaacaatga ctatggcatt ggattttgta
     1741 tgctttacaa ttttccatgc cataaataaa tccttcattt ccttttcggt cggtttagag
     1801 gaagttactg tttttaactc ttcaccttta ggaaggagct ttgaatcaat ttcctggact
     1861 aataaaccgc cggcaacctt tttaatatcg taggaatatt catcctgctt ttgtaatata
     1921 ttttctattt gcaaaagtct tatatttttc tttgacataa gtactgacaa agcttcttgg
     1981 ctgtatgaag gagcaacaat tatttcaaca aagattttat tgatttcctg tgcagtcctt
     2041 tcatcaattt ccctgttaaa tacaactatg cctccaaata ttgaagtggg gtctgcattg
     2101 aatgccttca tgtaagcttc ataaatattg tccccgcatc ctactccgca tggatttgca
     2161 tgcttgcatg caacgacaca aggctcgtca aattctttta agagttccaa agctccgttg
     2221 gtatcattta tattattaaa agacaactcc ttgccgtgaa gctgcaaagc tgaagccaaa
     2281 agaccgtcaa catttttaac ttctttataa aatactgctt tttggtgagg attttcacca
     2341 tatcgcattt cctggatttt ttcaaaagtc attgtgtatg tatcaggcaa agtattgtcc
     2401 tttctttctc ttttaatata agatgcaatc aatgtgtcat aatgagcagt atgagaaaaa
     2461 acctttgagc aaagataaaa tttagtgtct aaggaaactt ctttattttc ttttaattca
     2521 ttaagaacca attcataatc cttagggtca actataacag ccacatcctg atagttttta
     2581 gctgcggagc gaagcatcgt aggaccgcct atatcaatat tttcaattgc ttcttccctg
     2641 cttacgcctt cttttaaaac agtttgttta aagggataca aatttacaac caccaggtct
     2701 attggcttaa tatttaaatc taatagctgt ttcatatgct cagggtttga gcgcattgcc
     2761 agtaatcctg catgaatgtt cgggtgcaac gtttttaccc ttccgtcgag gcattcagga
     2821 aaatttgtta cttcggaaat tcctcttact ttaattcctg cctcatttaa ttttttgtga
     2881 gtgcctcccg ttgatataat ctcgactcct aatctttcta attctttagc aaattcgact
     2941 attcctgttt tatcggatac actaattaaa gctctcatca ttttactcct taatttttac
     3001 ttttcttccg cttatttgaa ttttcccttc gcagtaatac ttcacagcta aaggaagtat
     3061 tttatgctct attttaagta ctttttgctg taatgtttcg gcagtatctt catagcctac
     3121 ttcaaccgct tcttgaatga taattggacc cgtgtctgct tcctcatcta caaaatgtac
     3181 tgttgcaccg cttaatttaa cgccgtattt aataacttct tcatgaacct taataccgta
     3241 atagcctttc ccgcaaaatg ccggtattaa agacggatga atattaatta ttctattcct
     3301 atactcttca ataaatttag aagttagtat ttttagatat cctgccagta ccaccaattc
     3361 aacattatgt ttttttaact catcaataat agcatcattg aaattttcaa agctttcata
     3421 atccttttgc cttatatata gggcatcgat gtttttttgc cttgctctgt taagtccata
     3481 agcatctttt ctgtttgaaa tgacaacggt aattttaccc tttatatttc ctgtctcgat
     3541 attatcaatc aaggactgca gattagttcc tgagcctgaa attaatacac cgatttttac
     3601 ctgtggcata atttaagccc gccttccctt tttactgttc ttcctattat tacaggggtt
     3661 tcaccgacac ttttcaggta gctcaaaata tcgtccgcat catcttttgc aacagtcaat
     3721 actatgccga tacccatgtt aaatgtacca tacatttcat caatgtcaat gttccctttt
     3781 ttttgcaaca gcttaaatat gggaagaaca ttgatatttt tagtatctat atctgctgta
     3841 atgccttgag gaaacatcct tggaatattt tcataaaatc ccccgcctgt aatattagat
     3901 aaacccttta tttcaaattt agatatgagg tcaagtacca gcttggcata aattcttgtc
     3961 ggagttaata actcttctcc caaggttttt ccaagctcct ctacatagtc tgtaatatca
     4021 tagtttttgt gactgaaaaa aacttttcgc actaaggagt atccgttgct gtgaattccg
     4081 cttgaaggaa tacctattaa aatatcccct tctattattt ttgagccgtc gattattttc
     4141 tttttatcaa caattccaac acaaaatccc gcaatatcat attctcccac gtcgtaaagt
     4201 cccggcattt cggcagtttc tcctccaatt aaggcacagc ctgccttaat gcagccctcc
     4261 gatacacctt taacaatgct tgctgctttt tttggctcta gttttcctgt tgctatataa
     4321 tccaggaaaa ataaaggttt tgcaccctgg catataacat catttacaca catggctacg
     4381 gcgtcctcac cgatagtgtc gtgcttgtct gccataaatg caatcatgag ctttgttcca
     4441 acaccgtctg ttcccgaaac caataccggt tcttcatatt catccttatc gatttcaaac
     4501 attcctccaa aaccgcctat gtttgaaatt acacccttta tatgtgtttt tcttacatgc
     4561 tctttaatga gctttaccgt ttcataaccg gaatttacat ccacgcctga atctttataa
     4621 gtcagtttat cagtcataat acctccttat ataccgttat tgggtacttc catgggataa
     4681 tcgccgttta aacaagcctt gcagtagcct agatttgaac ctgtagattt gattaagttt
     4741 tctaaactca aaaaatgtac gctgtctgct ttaatatagt cttttatttc ttctattgtt
     4801 ttatttgcgc ctaacaattc ttttctgtca ggggtgttta taccgaagaa acaagaatgg
     4861 gttaccggag gtgaagctat tcgtacatga acctcttttg cccctgcttc cctgaccata
     4921 cttacaattt gttttgttgt tgtccctctt actatactgt catcaaccat aactacaact
     4981 ttatcctgaa caatttcctt taaaatgctt aattttgttt tgactccctg ctgtcttagt
     5041 ttatcagtgg gctcaatgaa agtcctcttt gcatatctgt tttttataat tccaagacca
     5101 tatggaatac ttaattcttc tgcatatccc aatgcagccg gtgttcctga gtcaggcacg
     5161 caaaatacca gatctgcttt cacgggagct tcctgtgcca acaatctacc tgatttatat
     5221 ctcgtctcat aaacatttac accatctaaa ttgctgtccg gtcttgcaaa atatacatgc
     5281 tcgaatatac acaatctttg ttgtgcatat ttggtggttt ttatgctgtt aactccttct
     5341 tcatttatta caacaatttc accaggctct atatctctta taaattcacc gcccattgca
     5401 gtaattccgc agctttccga agcaagcaaa tatccgtctt cccttttccc aaggcaaaga
     5461 ggtctaaggc catagttgtc ccttacgccg ataagctctt tatctgtcat aactacaaat
     5521 gcatatgctc cttttacaac ctccattgta gtttgtatag atttaattat actgcctctg
     5581 taatgtcttg aaattaaatt ggctacaacc tcagaatcag tggaagtctg aaatattact
     5641 ccttcatttt caagtatttc tcttatagaa tctgcattaa ccaaatttcc gttatgggct
     5701 aaggctatcg aaccttcttt atatcgcact actaagggtt gtgcattttg cttttcgctt
     5761 tctccagatg tggaatatct gtcatgccct attgcaatat tgccttttaa actatctatt
     5821 accttttgtg aaagaacatc ggcaaccaat cccatatcct tatggaattc aattcttttt
     5881 cctgtatgaa ttgcaatacc tgcactttcc tggcctctat gctgaagtgc aaataaaccg
     5941 taatatataa gctgacttgt aatatccgga ttgtctgaat atactccaac aaccccgcat
     6001 tcctcatgta atttatcttc ttctaaattg aacatagtat ctccttcttg tcattggtat
     6061 gaatgtttgt ctcgtcatct ttatcctgtg ctcaggatga catcaatttt tcgtttaatt
     6121 tcttgtcttt ttcaaatact ttttcagcca tttgtttttt ataatccaaa aacttattcc
     6181 ttaaatcttc gtatttcacc gataatatct ggatagccag taatgcagca ttttctgcgg
     6241 catttatgcc cactgttgca accggcactc ccggaggcat ctgtactatc gataacaagg
     6301 catccattcc gtctaattcc gaagatttaa tcggaagtcc tattaccggc acagtcgtgc
     6361 atcctgccaa cactcccggc aaatgggcag cctttcctgc tgcagctata atgacatcat
     6421 aaccgttttt atatgcattt cttgcaaatt ccaaagcttc atatggtgtt ctgtgggctg
     6481 atataaccct tacatcagcc ataacttcga aatctttcaa aactttataa cccttttcaa
     6541 ctaccggaaa gtctgaatca cttcccataa ctattgctac tttcattact cctccattat
     6601 ttaaaatatt ttacgcctga ttcaaatatc ggttggtttt tgtttccggg aatattttta
     6661 tataaatttt cgtccagtct ttcagaatgt gccatcttgc cgaaaaccct tccgtcaggg
     6721 cttgttattc cttcaattgc ttccacagag ccgttcggat tgtaaaaacc gtcataggtc
     6781 ggattattat ttaaatcaac atattgagtt gctatttgac catttgtttt caatttttct
     6841 atccattttt gatttgctgc aaatcctcct tctccgtgag acacaggtaa gtagtgggtg
     6901 tctcccacac tgcacattct taaccacggg gataggttgc ttacaacctt tgttttaaca
     6961 aatgttgcta tatgccttcc tatcttatta taagttaaag tcggataatc attctccata
     7021 tcagtgattt cgccaaatgg taccaatccc aatttaatga gtgcctggaa gccgttgcat
     7081 ataccaataa tcaagccgtc tctattgttt aacaaatctg taactgcatc ctttaacaat
     7141 tcattcctga atactgccgc aataaacttt ccggagcctt caggttcatc tccggcactg
     7201 aatcctcctg gaaatgcaat aatctgtgac tgccttatga gtgaattcat ttctgttatt
     7261 gaatctttaa tatcctgtga gcttaaattt ttaaaaacaa aaatatctgc ttttgctccg
     7321 gctctttcaa aggctctctt ggtttcatat tcgcaatttg ttcccgggaa taccggaata
     7381 aatactcttg gttttgcaat ggatatggag gatttagcaa tatgtttaga attaaaacta
     7441 tagttctcaa tctctccggc tccatccttt ttcacaggga atatattatt taaaggttta
     7501 agccaattag tcttgatttc ttccaaggat atgcaaatac cattaaccca tatttccttt
     7561 tcatttattg tttttcctat ttcaatgcaa tccgcaaatg gtaaaaatga gttgtcgacc
     7621 tctaatacaa tgtcgccata gcttgagtca aaccaatcat taccaattgt aaaatcactt
     7681 ctgaatccta tttcatttcc caaggtcatc ttgcttatgg cttcagcaat ccccccgtat
     7741 cctacggtat gcgcacttaa aacctttcct gtttttattg cttcatgaat agccgaatat
     7801 tttataccga tatcttcaaa atcaggaata ttattttcat cttttttaat ctttattaat
     7861 acaaccttgt tgtttgctct cttgaattcc ggtgaaataa tattttttac atctgaaact
     7921 gttactgcaa agcttattag cgtaggcggt acattgagtt cgttaaatgt acctgacata
     7981 ctgtcctttc cgcctattga gggaatttta agttcatgct ggaccttaaa tgctcccaac
     8041 aaggcgctta acggttttcc ccatctttca ggctctttat tcaacttttc atagtactct
     8101 tggaatgata gcctaatatt tttatagtct cctcccattg ctacaatttt ggcaatagaa
     8161 tgtattaccg cgtacattgc tccgtggtaa gggctttcct tagaaagata agggtcaaat
     8221 ccataggtca tcaacgagca ggtatttgta attccttctt ccacaggaat tttggcggcc
     8281 attccttctg caggcgtaag ctgtgtcttt cctccaaggg gcactaatac tgagccggca
     8341 cctactgtcg aatcaaatct ttcaatcatt cctttttgac ttgcaacatt caaatcattt
     8401 aaattttcaa gccatatttc tcttatatta ttacttttaa aacttctttt atattttgag
     8461 ttaagaggtt cttttattct tgcatttgtt ttttgcttta caccgttggt atccaaaaaa
     8521 tccctgctaa tatcaagtat cgtctttcct ctccatgtca ttttaagcct tctgtcatca
     8581 gtaacctctg caaccactgt tgactcaagg ttttcttcat tagcatattc attaaattta
     8641 tctgcatctt cttttcttac aacaagagcc attctttcct gggattcgga aatagctaat
     8701 tcagttccgt caagtccgtc atattttttt ggcaccaagt ccaaatttat gtttaaaccg
     8761 tctgctaatt ctcctatggc aacagaaact cctcctgcac caaaatcatt gcattttttt
     8821 ataagtgtgc ttacttcttt ttttctgaac agtctttgaa tttttctttc cgtaggagca
     8881 ttaccctttt gaacttcaga acctcttaac aatattgatt cttccgtatg ctccatggat
     8941 gaaccggttg ctccccctat accgtctctg cccgttcttc ctcccaatag tattacgaga
     9001 tcaccttttt taggagtttc tctttttaca ttttctctgg gtgctgcagc aatgactgca
     9061 ccaacctcca ttctcttagc aacataatca gaatgatata tttcatttac aaaaccggtg
     9121 gcaagtccga tttgatttcc gtaggagctg tatcctgctg ctgcttccgt agtaatcttc
     9181 ctttgaggaa gctttcccgg taatgtatct tcaatttttt gtctggggtc agcacttcct
     9241 gtaacacgca ttgcctgata tacatatgaa cgccctgaca gagggtctct tatggctccc
     9301 ccaaggcatg tggctgctcc tccgaagggc tcgatttctg ttggatggtt atgtgtttcg
     9361 tttttaaaca ttaaaagcca ctcttcattt ttcccgtcta tatctgcatt tattataatg
     9421 ctgcacgcat tgatttcttc cgatatttcc aagtcatcca aaagacctgc ttttcttaat
     9481 tcctttacac ctatagttgc caggtccatt aatgtgatat cttttttgcc ttcataaaca
     9541 tactttctta cattcaaata ctcatcatat gtgtttttta tttcttcagt tatttttcct
     9601 ttttcaaatt caatattttc tataatcgta gaaaatgttg tatgtctgca gtggtctgac
     9661 caataggtat caataacttt aatttcggta attgtcggat ttcttttttc aatattttta
     9721 aagtattcct gacaaaaagc taaatcttca aagctcattg caagccctat atcatttcgg
     9781 aactcttcta attcttgttt agatttattt ataaagttta ttgtttgtac atcttctgcc
     9841 atcggatatt catcatccaa agtatgcggt ttatctaact gcgcttctct ggaatcaaca
     9901 gggttaatgt agtatttttt aattctatca atatcatctt ttgataaatc cccttcaaga
     9961 acaaaaactc gtgcatattt aacggtaggt ttttcttttc cggtgactat ttgtatgcat
    10021 tgagccgcaa aatcggctct ttggtcatat tgtcctggca ggtattcaac ggcaaaaaca
    10081 tgtccttcaa gctcaacatc ttcaaaatac acaatatcct gattaggctc tgaaaaaaca
    10141 gtatttacac tttttttaaa atcatcttca tcaatatttg ataccgtata tctgtttaat
    10201 attctaaggt tatttaattt ttcaatattt aaattttcct ttaagttatt taataaattt
    10261 aatgcttcgg tgttaaaccc tgctcttttt tcaacaaaca ccctcttaac gtcaaagttc
    10321 ataaataaac cttccttttt atcattttac aaaattataa taacaaatat aaaaatcctt
    10381 gtcaacggtt tatatagtaa tttcaacaaa aaatatattt catgttcgta ctataccgaa
    10441 ttattaatta gacatgtccc tttatattcg taatattatg aaccttgcaa aatattccaa
    10501 ttaataaagg gaatgggggt taattataaa aaaaacagta accttacgat tactgttttt
    10561 attattaccc ttcctttttt attcaatata ctctctagcc ttttcttcac ctctgagaac
    10621 tcttagagcg ccgaaagcca aagcttccat ttcattttca ccgggcatta tttctacatg
    10681 agatataaaa gatactctgt tttttatcca ggaagtaagc atctcagaat gtgcaatacc
    10741 tccggtaaga attataacat ctaccttgcc ttctaccact gtagccagtt ctcctattcc
    10801 ttttgctatt tgatatgcca ttgcttcata aatcaatttt gcatattcat tgccctcttt
    10861 aatcatagat tcgacttctc ttgcatcaac ggtgtttaaa tatgctttta agcctccgct
    10921 tcctcttagc tttcgctgca ttgttttttt atcaaattta cctgaataac aaagatttat
    10981 cagctctctg cacggtactc ttcctgctct ttcaggtgaa aagggtcctt catcatcaga
    11041 aaccaagtct attatctgac ccttgtgatg aatattgatt gaaattcctc ctcctaaatg
    11101 cgcaacgata gtgttcagct cttcgtaact tttgccgata cttttagcgt attttatagc
    11161 cattgccctt gtatttaata catggcttgc gctttttctt ggtatatcag gcataccgga
    11221 tatacgagaa atgtctggaa attcatctgt catcacggag tcatatatat atgaaggtac
    11281 gccggcttcc tttgcaatac cgtaagcaat cggagctgca agatttgatg catgctccat
    11341 tacgggacga ttttccaccc tgtcgagcat aatatcatta ataacatatg ctcccgattt
    11401 taccggaaga agcattcccc ctcttgcaac aacacaagat aattccttaa tgtcaaattg
    11461 cttttctttt aaataggatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    11581 cttttcccat caatatgggt gcggaagcaa tttcgaaatc attgattaca acaactccgt
    11641 caggctttaa gtattcaagc catctcaatg tttccatcgt ttcaaaggaa accagaatat
    11701 cggcttcacc gattccgata ataggcgaaa atacttcact tccatatctt acctgagtcg
    11761 aaacactgcc tcctctttgt gccattccgt gaatttctga cattttaacg tcataaccgg
    11821 ctgtcataag acctgttgat aatattttac ttaccaatat tgttccctga ccgccaacac
    11881 ctaccaataa aatattttta gtatccttca ttttattcac ctaccttact gattgcctta
    11941 gtggggcata actgtagaca aagctcacaa ccgacacatt gaaatttatt tattgaagat
    12001 ttcttaatgt cttttttaaa cactattgcc ggacaacctg tacctacgca aattttacat
    12061 cctatacatg tttcttcgtc aacttcgcaa taaccttgaa taggtccgaa ttcttccagg
    12121 tcttcttttg tatatttctt aagagcacat gggtatcttg aaattattac cgatggttcg
    12181 tttaaatcgt aagcccaatt aagagcagct ttcatttcag cttgattcat cggatttact
    12241 gtttttatgt ttttaacacc taaagctctg actataggct caatttgaac cgcatcagta
    12301 ctatctccct gaagcgtata gcctgttccc ggattatcct gatgtcctgt cattgctgtt
    12361 attctattgt ctaaaataca agttatggta tttcctttgt taaacaccac gtccagcaag
    12421 cttgtcatcc ctgaatggaa gaatgttgaa tctcctataa ctgcaactac tcttttatca
    12481 tcattgttcc tttcaaatgc ctttttagca ccatgtgcca tgcttatgct tgcccccata
    12541 catacacatg tatccattga atttaacgga ggtgctccgc ccaatgtata acatcctata
    12601 tcacctgtta ctatagtgtt ctttttcttt gacaattgat aatagaatgc acggtgagga
    12661 caaccggcac aaagtgtagg aggtcttccc actgctattg ttctgtcata atcaattatt
    12721 tcattttcag taccgaaaat tgattttttt acaatatccg gattaagctc ccatatattg
    12781 gggatttttt ctttaccgat acatttaata ccggcagctt ttatctgttc ttccataaaa
    12841 ggttctaatt cttcaactac ataaagagtt tcaacctctt ctgcaaattt tttaattttt
    12901 tccattggaa gcgggaaagt aaatcctaat tttaaatatg atgcatcctt tccgaatact
    12961 tcttttgcat attgataagc aactccggcc gaaataacac cgattttttt gtcattccat
    13021 tctatataat tcaactcagt ttcattcgaa aacttttcaa gttctttaag ctttttctca
    13081 actaccacat gtgccgcttt tgcattagca ggtgccataa tatattttgg cgcatttttc
    13141 acgtatggtt ttacaggaac ttctattctt tcgcctgttt caacaattga tttactgtga
    13201 caaactcttg tagtcattct aagcattacc aagacatcat acttttcgct tatttccatg
    13261 gcttttatta ccatgtcctt gcattcctgt gagttgcttg gctcaagcat agctaccttt
    13321 gccattcttg cgtaatacct gttatcctgc tcattttgag aagaatgaag ccccgggtca
    13381 tctgcgctta ccagtaccat tcctccgttt acacctgtgt aaccgattgt gaaaaacggg
    13441 tcggctgcaa cattaagccc aacatgtttc atagctgtta aagttcttgc tcctgcaagg
    13501 cttgctccaa atgccgactc aactgccact ttttcatttg gtgcccattc tgctgttatt
    13561 tccttgcgaa gagctatgtt ttccagtatt tcagtactag gtgttccggg atatgctgca
    13621 gcaaaatgaa ttcctgcttc ataagcaccc tgagcaactg cttcattccc tgtcattaaa
    13681 actttcattt tcttcctcct ataacttttt tctagaaatc tattttgcac taatcatagt
    13741 taaattcata atttcatttt caagactttt aatattgttg ttaatctttc tgaatttgtc
    13801 gatgtctata tcatcgacca acagacaacc ttcagttttt gctaatgcat tttcaaatgc
    13861 cttctttagc tcttcaactt cttttaataa ttcctgtata tacttgtcct ttattttttg
    13921 tttttcatct accagtacta cttcatttat caaatttttc tttttaattt cataaatgac
    13981 gtcgtattcc ggtattatac agtctatatt aaatttttct tttacatcaa tgcaaaagga
    14041 ttcttttgat tccttttctc catgaactat aaagattctc cccggctttt ctttaaagct
    14101 gccaatccag ttgagaagag caggcttatc ggcatgtccc gaaaaccctt caacattgta
    14161 aatttcagca ttaaccacta tatcttcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnttt tcaaaatcca aggggttgtc ccctttcatt atataatcct ttgcttcctt
    14341 atcaaaagca tagctgtttc ttttgaatac ttctgttgcc ttcattgcta gcgggctgtc
    14401 tatatagacc ggtatttttc ttaattcatt ttccttagta ccgatttcat tcaaatggct
    14461 gtcataatat ttattcagct cataaatcag ctcctgtgtt ctgcctaccg caaaggacgg
    14521 gataattacg ctgcctcccc ttttagttgt ttttaatatt atattgataa gctcttctgt
    14581 tcttttttcg atgttttcgt gaactctgtt gccataagtt gcttccatta ttaaataatc
    14641 agccctgtct atttgagcag ggtcttttaa aataggtttt tcacgcattc ctatatcacc
    14701 ggaatacacc agcttaattg tatctttttc atctttaaac catatttcaa tgattgaaga
    14761 gcctaaaata tggccggcat cgttaaattt cacagttata ttttcatcaa tatttataat
    14821 ttggtcgtat aagacaggtt taaaatactg caagctatct aatacatctt cctgcgtgta
    14881 caaaggctcc actaattttc tccctgaacg ttttgccttg ttatttttcc actgtgcttc
    14941 tgcttcttga atatgtgctg aatccaagag cattatctca caaagttcat aggtaggctt
    15001 tgagcaataa atggctccct taaaaccctt ttttaccaat aaaggaattc ttccgctatg
    15061 gtcgatatga ctgtggctca ataaaaggca atcaatatct gccggattta ttgcaaattc
    15121 ttctgcattt agctcctcgt ccttgccttg atacataccg caatcaagca tgatattttt
    15181 gcccctgatc tgaattaagt ggtttgaacc ggtgacaccg ttgacagcac ctaaaaacct
    15241 gattttcatt atatacctct ttccggttaa aaataaaaaa gtcttaaaaa atattaacct
    15301 atattaagta tgacaaaaaa acacatttat gtcaatggct tgtaccaaaa aacccattgt
    15361 caaaatatag tgttaattta taaatattta aggtgtttcc aaattattgg aaacacctat
    15421 cagtattaga caatctacat ataccgcagt ctcattcacc aatttattat atttggggac
    15481 attcctcatg ccctcaaaga ctaactccat agtagaggtg tatcccctta cctatggtcg
    15541 cataaattgg gaattcgttg cgaatgacct accaaccatc tgttcttctc tttagctcga
    15601 acatatgggg ttagggcatc ttcaattctc catttgtctc gataaaaata tacttgctgt
    15661 ttttattaat ttttcttcaa cttcagacat cttagagccc ttatccttcg ataagagtat
    15721 aacggctcct atcgggtctc catgtaccat aatcggtgca atcacctgac ttgtataatt
    15781 gtcaggatta aattcattgg cagtaattct gattggcttt gaagttgcac tggtttgata
    15841 tatttctctg ctttcaatta ttttctctaa ttcaggacta agcctttttt ccgcataatc
    15901 tttctttgag cttcccgaaa cggcaattac gccgtctcta tccgtaatta ttgcaatatg
    15961 cctcgtagtt tcaaataatg attccacata ttctcctgca aattcagtca actctccaat
    16021 aggtgaatat ttcttcaata ttatttcacc ttctctatcg gtgaatattt ctaaaggatc
    16081 gccttctctt attctcaagg tccttcttat ttctttagga ataactactc tgcctaagtc
    16141 gtcaattctt ctgacaattc ctgttgcttt cattacttac gttcctccaa ttacatattt
    16201 ttatattttt atgacatttc ttttaatatt ttctattatt tacactttca taaattttat
    16261 ccaacagata aaataatgtt gaaaatattt ttaaaaaaga aacattaatt cattataatg
    16321 gaaggaatta aaaatatcaa cattttttta cttaaattaa caaattttta attttttact
    16381 attaccgcat catttttcag ctgttctaaa agatcttcgt atttgttcaa tttataatat
    16441 tgaataagct tttctttttc agattctacg gtagtttctt catcaacaat cttatctgtc
    16501 agcttaataa tatgatatcc aaaatcagat tgtacaggct ggctcacttc tcctatttcc
    16561 agtgcaaatg ctgcttctga gaaaggctta accatatccg tatatttaaa gtaatccaaa
    16621 tctcctccgt ttgctccgct tgctgtatca atggataatt cttttgcaag ctcaccgaag
    16681 tcttcaccct tattaattct ttcaactatt tttacagctt cttcttccgt atccacaagt
    16741 atatggcttg cttttatcag aacatttaat ttcatttctt caaatagatt tttcagctct
    16801 tcattatttg gatttaagga atcaattttt gatgttatat aattattaat taatatttct
    16861 ttggttaact cttgtataat aaaatcttcc gttatacctc tttgctccat aaaactttta
    16921 tattcttcat cgttttcgaa attaatttta aagttttcca atgaatgctg tacttcttct
    16981 tcacttgcct ttattccttc tttttcggca gcatcaagca gaagcaatga tttagtcatt
    17041 tcatcccata cgtattcttc ataaatttct ttataagtac cccttggtgt ttctgtttcc
    17101 catgcatctt cgcctaattg atactcaaca taatccttat aatatgccaa tgcatcatca
    17161 taatctttct gcataatcgg cttgccgttt acagttgcca ttacaccctc tttatcgaca
    17221 tctttgccgc ctgagtttga gcaagctgta aatactaaag ctatgcataa taaagtaatt
    17281 aataataatg attttttaaa tttgttcatg tttttcctcc acagcagttt ttcctgcttt
    17341 aaaattattt agtgtttcta aaaactcttc cagttcccta agaattttca tttgctcaag
    17401 agaatccagt ttatatttaa taatcggttc gttagttcca acaaagctta ttttttcata
    17461 ataattcttt attaaaaact caatgatatc ttgactcagg taatctttcg tcatttctat
    17521 gtttacggta ttgcctgcct gagctattga ttttatttta attcttttgc tcaatgattt
    17581 aatgtatgat atttttaaaa gattatctac ttgaacagga atatttccaa acctgtcaat
    17641 aatttcttca gttatatcaa atatatcatc tttattctct gctgctgcta tttttttgta
    17701 aatttctatt ttttgctctt cattttcaat aaatgatgaa ggaatataac cgtcaacatt
    17761 taaatcaacc gacgtttcaa tatcttcctc aacttgtttt ccttgaattt ctttaactgc
    17821 ccggtctaag aacttaacat ataaatcata tcctattgcc agcatatgac catgctgctc
    17881 cggacccaat atatttccgc tgcctcttat ttccaggtcg cgcatagcta ttttaaatcc
    17941 tgaaccgaac tcggtaaatt cctttattgc cttaagtctt ttatcggcaa cttcagacag
    18001 cattttgtcc ttctcgtagg taaggtatgc aaaagcaact ttgtttgacc tgcctatcct
    18061 tccccgaagc tgatacagtt gtgaaagtcc caaataatcg gcattatcaa taatcaatgt
    18121 atttacattg ggtatgtcca ttccggtttc aattatggtt gtacaaacca aaatatcata
    18181 ttccttattt acaaattcaa gcattatatt ttcaagatgt ctttcgctca tctggccgtg
    18241 agccactgct attcgtgcct caggaagcag ctttctcaac tttgctgctg tttggtctat
    18301 gtctgccact ctgttgtgta cgtaataaac ctgacccttt cttgaaattt ccttttcaat
    18361 tgcatccttt atcaatcctt cgttatattc aataacataa gtttgtatag gaagtctgtc
    18421 tccgggcggt tcagatatga tgctcatgtc ccttacacca atcatagaca tatgaagtgt
    18481 tcggggtatt ggtgttgccg acagcgtcag aacatcaata tttgatttaa gctgttttat
    18541 caattcctta tgtctgacac cgaatctttg ctcctcatct attatcaaaa gtcctaaatc
    18601 cttaaattta agctctttgg aaagaagttt atgtgttcct attacaatat caatcaaacc
    18661 tttatttaaa tcgttgatta ttttgttttg ttggtaatgt gttcgaaaac ggctcagcat
    18721 ttcaatttta atgggatatc ctctgaatct ttcaactaaa tttgtataat gctgctgcgc
    18781 caatatggtt gtaggtacca acatcgccac ttgctttgaa tccataactg ctttaaatgc
    18841 ggctctcatt gccacttcgg tttttccgaa cccgacatcg ccgcaaagaa gtctgtccat
    18901 gcaaataggc ttttccatat cttttttgat ttcttttacg caacgaagct ggtcttccgt
    18961 ttcttcaaaa ggaaatttat actcaaattc tttctgccac tcggtatctt ttgaaaaagc
    19021 atatcccttt aatacctttc tttcagcata cagcttaatc agctcacttg ccatattttc
    19081 tatggctgcc tttgagcgct ccttggtttt tatccagtct gagctgccca ttttgttaag
    19141 cttgggtttt tcggaatctg ttccgatata cttttgtata agagacattt ggtcaacagg
    19201 aacatataac ttgtcttcac ctttatactt gatgcacaaa taatcctttt gaatattctg
    19261 tacttctatt ttttcaattc ctatatattg accgattcca tgatgctcat gtactacata
    19321 atctcctact tttaaatcgg tgaaaatttc aattgcagca cccttgcgtt ttttcgtctt
    19381 gcgggatttg cgcttgacag agccaagcac ctcattttct gtaagcacaa ggagcttatt
    19441 gtcgtagtat tcaaatcctt ttgttaattc tcccggtgca attaccactt gtccgctttc
    19501 tgctgcgttg gtatctttag acaatgtggt tgtgcattca taatcattta ataaattata
    19561 aagtttactg cagctttcat aggtgcttag tataatatct actttatagc ccttgtattt
    19621 cagcctgttt aaatcttttg acagctcttc aatcttgccg tagtaagaag gagcttcttt
    19681 aaaaagcata tgaacggaat catctgtttt aattattttc tctttaccgt ttatagaaat
    19741 gaaaggtttt ttggaaatct tcgtcaatag ttcatgctcg cttatatata cttcactttg
    19801 atttgaaaaa atttctcctc tttcaaataa ttcagtatat ttcagctgaa agctttcatt
    19861 taaatcatta agttcttcaa atattctttc aggctcatca agaagaagaa tagaatcatc
    19921 ctcaaaataa tctataatac ttgaaaaaga ttcctttata aatggtatca aaaggtcggc
    19981 attttcaata cccataccgc ccataagctt atcttgatag ctcttgtaaa ggttctttaa
    20041 attcttaaaa gcttctttgc ttaagctttg gttttgactc tgcttatcat aatccttttc
    20101 aatttcagat aaaataattt ttatttcttc ttttttaaac aagatgtcgc tgcaaggaag
    20161 aatcttagca gatttaatgt tcttaaccga gcgctgtgtt tttaaatcga ttaatcttat
    20221 tgaatctact tcatcatcaa aaaattctac cctgaacgga tttgcttctg aaggagaaaa
    20281 aatatcaata attccgcctc ttacagaaaa ttggccaacc ccttcgatgg catccactct
    20341 ctcatattta agcttaacca aattgcttct taattcattt aaatcataac tgtcgccata
    20401 ttttaattct ataatgcttt ccttaaatct atctttagac attactcttg taattattga
    20461 attaattgaa gcagttacaa tacatttccg cttatttaaa atgttattta acagatttgc
    20521 ccttttatac tccacatcct tgcttaaagc atcaacatta tacaaaataa attctttagg
    20581 ctgaaggcgt tctgaatgct ctgtataagc tctaataatc tcttcgtact ttttcgattt
    20641 tgcttcactg cttgttagaa ttaatatttt atcggatgta tctaaaagat tgcatgctaa
    20701 ataagctaat ccttcctcat tgagtccttg gataagctgg cttttttcct tattataatt
    20761 tgaacaaatc tttttgaatt tttctgttct ttgaaactgg tcaaacaaaa ttctgttcat
    20821 atttttcctt tatttgtcat tgctgataaa ctagttatat ggtacacgta ccttacgtta
    20881 aagtgtgtcc cccttcatat tgaatttctg cattgcgctg tctataccat tttcaataat
    20941 ttctaccgct gcttcagctg ccctttcaat aatgggagtg actatatctt tttcattcct
    21001 tgaaaatttc tgcagcacaa aatctgccaa atccatttcc tcatttcttc ctattcctat
    21061 tcttatgcgc ggaaactcgc tcccgatact gtcaactatt gatttcatcc cattatgagt
    21121 gccggcactt ccactcggtc ttattcttaa agcccccagg ggtatatcta tatcatcgta
    21181 aataactatg agatttgacg ttgaaatttt ataaaattta actatttctg ctatggcaat
    21241 tccgctttta ttcatgtagg ttacgggttt aacaagcatg actttttctc cgtttatgga
    21301 agtatcccca tatacggagt taaattttag tttattcaat ttagtattaa gttttccagc
    21361 taaatattct actgccatat aacctgcatt atgtcttgag cttgcatatt ccttacctgg
    21421 atttccaagt cctgcaatta catacatatt aacctcttta aaatctgttt ttgcctcttc
    21481 cttctttatt gacctgccta gctcttgcaa ttgctaaatc tccctctttg acatcttccg
    21541 taatggtaga gcctgcagcc acatatccgc ctttttcaat agtaacggga gcaactaaat
    21601 ttgaattgct tcctacgaaa gcaccgtcgc cgactattgt tttatgcttc ttcttcccat
    21661 cataattaac aaatacaact ccgcatccta tattaacatt tttacctacc tctgcatcac
    21721 ctatgtaagc caaatgagat gattttgaat tgtcgccaat gattgatttc tttacttcaa
    21781 caaaatcacc aatttttaca tttttaccta aattgctgcc gggtcttaaa tatgcaaatg
    21841 gtcctacatt tgttccgtca tcgatataag attcaataac ctttgagctt tcaacggtta
    21901 cattatttct tacggtggaa tcaattatcc ttgagttagg tccgattgtg cagttgcttc
    21961 ctataaccgt tcctccctcc aaatatgtat taggataaat aaccgtatct ctgcctattt
    22021 taaccgaatc atctatatat gtgctatccg ggtcaatgat tgtaacacct gaatacatat
    22081 gtctttgatt tattcttttt tgcattattt ttgttacttc agcaagttgt accctgtcat
    22141 ttacagcctt gatatcatct gagtcctcta atagccaccc tcctatatta gcaccttcat
    22201 tatttaaaac ctttatggta tccgtaagat aatactcatt ctgtgagtta ttcttggtaa
    22261 ttttcttcaa agcatatttt aagagctcac ccttaaaaca gtatatcccc gtatttattt
    22321 cctttatatt tttaatttct tcagatgcat ccttttcttc tactatttca tcgatagaca
    22381 tatccgactt tcttactatt cttccgtatc cccttggatt atctaaataa gctgtcatta
    22441 tcgatgcagc acagttattt tcaaaatgat agttcataaa gtcaattaat gtttcttttt
    22501 taacaagcgg tccgtcagca caaagtatta aaaccgtttc cccgtcatcg atgtggtttt
    22561 ctgcagccat aacggcatag cccgttccat agggatgccc ttcaccgata ggctgttcaa
    22621 cagtttgtac attcatatcc ttaatattgt gtccaacttt ttcttttcca tgtcccaaaa
    22681 caacaatatt tttctctata cctgaattta aagatgcttc aattatgtat tttatcattt
    22741 cttttccaca tactttatgc agcgttttcg gtagatctga tttcattctg gtaccttcgc
    22801 ctgctgccaa aattattgac atactcataa taaattctcc ttaatgcgaa tctgtaatac
    22861 tgagttatta tatatcataa tatttagtaa gtcaaaaaat attcagggac attactctaa
    22921 tatttaaaaa aaattgatag aggaaattca ccaattttga ttcatattac ctttagacac
    22981 aataaaaggg tctatgaaag acccgagtac tgttgactgt aaatcatcag taacagcgct
    23041 acataagagc ggcttcttcc acgttttcgc tttcttctgt gcgcttttct tcatatgctt
    23101 taaatacagc ctctgcaaca agattccttg tttcagaatt gattggatga gctatatctt
    23161 taaactctcc gtccggcatc ttcctactgg gcattgctat aaacaaccct tcttgacctt
    23221 caattatttt aatatcatgt accacgaaac aatcatcaaa ggttacggaa acgattgcct
    23281 tcatcttgcc ctcagaatta atttttctta cccttacgtc tgatattttc atactgtcac
    23341 caccttcccc caataattat gtccaatttt attcattata ctataaatac ccaaaaatag
    23401 caaaaaaaca ttttcataaa tatatgaaat tattttaaca atttcggatt tggttttatt
    23461 tcgataacac cttcttttat acttttatta ttaagtatca ataatgaagt ataattatct
    23521 actaatttct tttccggctc tatagtttca ataacaacag cagtccctac aacatttgca
    23581 ttaaattccc tcatcatatc ctccattcct ttgcaggttc cgccgccttt cataaagtcg
    23641 tcaactatta gaatttcagc attttctttt aaagacctct ttgataatga catggtttga
    23701 atattgtttg atgaacccga aacatagttt atgctgactg ttgccccttc tgtaattttg
    23761 ttgtttctcc tgattacggc cacaggaaca ttcagctttt ggcctgtcat aatagctacg
    23821 ggtatacctt tcgtctctac ggtaactata taatcaatat gtttattact aaattgtgaa
    23881 gaaattactt cggcaatatt cgacacaatt cgagggttgt tgaaaatatc ggtcatataa
    23941 ataaaaccgc cgggcagtat tcggtttttt tgcgaaaata atgagcataa ctgctcaagt
    24001 atttcattta attccttctt acccaatata ggtataaaca taactccgcc tgaaactcct
    24061 gaaatggttt caatcctgcc aaaattcatc tctgcagcca attccttgac tattgccaca
    24121 tcttcactta ttgttgattt tgctgcattg aaaatttcac aaaaataatt atatgaaaac
    24181 aaacaatttg gtttttcggc aaaaattttc atcaatgcac cgattctttg atttcttttg
    24241 tattttttca ttttatcacc agcgaatgtt tttatataat tatattacaa acgttcgtat
    24301 aaatcaagac ttaattcagc tgtcctcctg caaatattat tgaggtttca gccaaaatct
    24361 ccatggggtt aataaagttt ccttccaatt tatatatata ttgtgctgac gaaagctcag
    24421 tgcagcctaa caaaaatacg tttgctccca attcctttaa ttcatctgcg cattcaaaga
    24481 aaagacttgt acctgcatcg taattgcctt tttttataac atcataaatg aattccatta
    24541 ttttttcctg tgtttttatc ggtacaactg tttttatccc caactcggca taatattttt
    24601 cataaatttt tgttttaata gttccatcag tagccattat tccaactaca gcatcctttg
    24661 catatttgtc atatgtatgc ttaactgcct cttcaggcat gttaaaaaag ggaatgctga
    24721 tatttttctt tatttcctct ataaagtaat gtgcagtatt gcagggcatt attaaaaagt
    24781 ccgcaccgct gttttcaagc atcttcgctg aattaaccaa ttcatttacc ggactttctt
    24841 cattaccgag tatggctaaa gttctgtccg gaatttttgt attgttatca attaagatac
    24901 gaatgtgatc ttggtccttt tctgcctttg ttcttaaaac aattctttca aataagtgta
    24961 ctgtggcaag aggccccata ccgcctataa ttcctattat tttcatagtc cacctcataa
    25021 tttttattgc tagattatat catattactt acattgtgac aaggggacgg gggtgttgtc
    25081 atcaaagatg acaacacccc cgtccccttg tcattaatta acataaatcc aaataattta
    25141 ctccgtcttc aggtacaata atttttatgc tgccatcatt tttcatccct acaataaaca
    25201 aggtataatg catgtaaggt gcagcatcta tccaatgatc taagatttca gtgccgtcgg
    25261 gtttctttaa ttgcaatgta tatcttcccg atgggattgc cacattacac gatacttttc
    25321 catacttaat atcagaaaat agaatagttc cgtccgacgc cgacattact acttctggtt
    25381 catcgataca caaatttgcc aaccgcaatg ctgacatatt acttgtcata ttatgtgctt
    25441 tttgctcagg taccattaaa atgcaaatat ccttcttatt ttcttcatca gctgagacga
    25501 ctgctgtgta tgccaagtta taatctatgt ctatatccga ttcaaataac aaattaccat
    25561 ttggagagtg tattaaaaca tggtaagaat tcggaggaaa cttaacatat tttgtgaatt
    25621 ctccgatttt taaattctct gccataagta tgtcatttac ctgtatatcc aaaggctgat
    25681 taacagtatt taacactctt acataagatt ttttgcaaaa acaacttgcc atcattgcat
    25741 aataacccat atatcctgta tatggatatg tattataata cttttttata tcaaacataa
    25801 attcacccct ctctccaata tatgaatttc ccctgttaat agttataaaa agatgtttga
    25861 caaatactga caataacata aaaaaaatct cccgaccttg tcgagagatt tcctaatatt
    25921 tattttacta ctatattgta tattttcccc ggtacatata tttcttttat ggttgttttg
    25981 ccttctatat atttaaaaat attctcatca gcttcagcca atttccttgc agtatttaca
    26041 tccgcatcct tcggcaataa aattttccct cttacctttc cgcttacctg gacaggaagc
    26101 tctataacat cctcaactgt tttttcttca tcccatacag gccattctgt ctgatttaac
    26161 atgccttcaa aaccttgttc ctgccatatt tcttcagtta tatggggagc cacgggataa
    26221 agcagtgtaa tgaatgtttt taattcatcc cttgtaatac ttccatcgtt gtaaatttca
    26281 ttaataaagc tcatcatggc agctattgca gtattatatt tcatagcttc gaaatcttcg
    26341 ctgacctttt ttattgtttt atgcataaga gttaaatgtt tatgagaata tcccttttct
    26401 tcatttacaa actcctgcat tttccagact ctgtctaaaa atcttttaca tccttttaca
    26461 cttgcatcat tccaaatagc atacttttca tagtctccga taaacataat gtatgttctt
    26521 aaagtatctg caccgtactc atctattata tcgtttggat ttaccacatt tcctctcgac
    26581 tttgacattt tttctccgtc tgcaccaagg attaagcctt gagcggtacg tttagcatat
    26641 ggctccttaa agggtacaac tcctatgtca tagaggaacc tgtgccagaa ccttgaataa
    26701 atcatgtgtc ttgtaacgtg ctccatccct ccgttgtacc agtcgatggg accccagtat
    26761 tctaactttt cttttgatgc tatttgctct ttattatggg ggtcaatata tcttaaaaaa
    26821 taccatgatg accctgccca ttgaggcata gtgtcagttt ctctttttgc atcttcatcg
    26881 catacgggac attttacatt tacccattca ggtatattgg caagcggtga ttctcccgtt
    26941 tcggcaggac gataattttc gacttcaggc agtaacaagg gaagctcttc ttccggcacg
    27001 ggaaccatac cgcaatgagg acaatgaata ataggaatcg gctcacccca gtatctctgc
    27061 ctgttaaagg cccaatcctt catcttgtaa tttacttttc tttctcctaa attattttca
    27121 accaaataat ttgtaatttt ttcaattgca tccttaactc tcaaaccgtt aatcataccg
    27181 gaatttacca atattccgtc ttctacatcc gtaaatgctt cttcttcgat atttcctccg
    27241 ccgattactt caattatggg aatattgtat ttttttgcaa actcccagtc tctttggtca
    27301 tgaccgggaa cacccataat agcacctgtt ccataaccca tcataacata gtcggcaacc
    27361 caaataggca cttcttcccc attaacggga ttaagagcag tcagtccttt tatttctact
    27421 cctgtctttc cttttgctaa ctgcaccctt tcaaattcgg ttttcttttt ggactcttcc
    27481 tggtattctt ttatttcatc cagattttgg attatatttt catacttttt aatcaaagga
    27541 tgttcgggag ccattaccat aaatgtaact ccgtacaagg tatccggtct tgtagtaaat
    27601 actcttaaat tgtcctctgt gtcctttaat ttaaaatcaa cttcggcacc ataagaacga
    27661 cctatccagt tttcctgctc aattcttata cgcggcaaat aatcaacttc atccagaccc
    27721 tctaaaagct tatccgcata atctctgatt tttagaaacc atacatcttt ttccatctgc
    27781 accacctggc tgccgcatct gtcgcatatg ccgttttgcg aatcttcatt tgcaagtaca
    27841 accttacagc tgttgcagaa gtttacatat gttttatcct tataagccag cccatgctta
    27901 aataattgaa caaatatcca ttgagtccat ttatagtatt cagggtctgt cgtatcaaca
    27961 cttctggacc agtcaaatga atatcctgca gcattaagct gtgcagtgaa aatcttgata
    28021 ttatcatccg taacctttct tggatgtttt cctgtctgca ttgcataatt ttccgtagga
    28081 agaccaaaag catcccatcc tatgggaaac aatacattgt atccttctaa tctccttttt
    28141 cttgatatta cttcaaggga tgtataggca cgaatgtgcc ctacgtgcag tcctacccct
    28201 gaaggatatg gaaattccac aagaccataa aactttttct ggtctgtatt gtctttcgct
    28261 ttaaaagttt cattctttct ccatatttcc tgccattttt tttcaatagc ttgtggatta
    28321 tatgatttca tttttcgcct ccatgttttt actaaatcgc agctccataa ccaatttgtt
    28381 gacaataaaa atcttcctcc cgattgggac gaagattccg cggtaccacc caaattaggc
    28441 ctaagcctca ctcaatatta taacggtatc accgtttgaa actacttttt tcgcttcaaa
    28501 tactcccgga cgagttcagc attagcaata ccgtctcaca ccacccgacg gctctctttg
    28561 attgtttaca gcctactact tccattcatt gtattttatt aacttttcat tcgattatat
    28621 cagaacaaaa aacgtattgc aataacaaaa ttgtaaatct ttacaattta gctttgccgc
    28681 atctgtttct gtgacagcat cttgtgctgt gaaaatctcc ttcgccgtcg caaagcttat
    28741 aatctccgcc ttctattcgc aaaactttcc cgtttaccag ggattctgca attttctttc
    28801 tggcttcctg gtaaataccc tggacggtag ttcttgccac atgcattaaa cttgcacatt
    28861 cctcttggga aaggccttct aaatctataa gtcttatagt ttcatattca tcaacggaca
    28921 taataacaac atgttccttt tctaccgcaa aatccaaagg tccaaagcga tttacatcag
    28981 gcaggctgca gacccttctc cattttctcg gtctaggcat taataacacc ccatttctta
    29041 ttatagctta tattatatct gatttatgaa atatgtcaat aaatcagaat atcaatcctt
    29101 tggattctca ttttcacagg gaacacatac ctggcatttg ccgcagccat aataaggttt
    29161 atgcttttcc ttaaccttat ccaaaaagtc ggaacacttt ttatggtctt ttccattttc
    29221 taaggatatt gcacctgcag gacaattttc tgcacatata ccgcaattta tgcagtattc
    29281 atatatatta ttatattttt ttatatccga ttttaatttt aattccgtca ctatacttcc
    29341 taatcttccg gccattcctt tttgagtaat tatccctttt gataatccaa aagtcccaag
    29401 cccgcaaata aaagctacat gcctttctga ccaattgctg gagaaaccct ctgacttgaa
    29461 ccttgaatcc aatccgggaa ccaagctttt atagccttct tcattcagtt tggaatttag
    29521 aaataagttg aattcattca taaaaatttg tccatcaatg cgtccgtgaa gccattcatt
    29581 tgaaggccag ctcatatctg ttctgttacc ttctctgatt tcatccgtaa acggaaagaa
    29641 ataaactata actgtatttg cactttgaag ccattccttc ggcatcataa aatgttctcc
    29701 gataactgaa ggtgatttta ataattgaaa accttcatcg tctgcagctc cataagcaaa
    29761 tattggttca tcaaaaattt tcaaacctgc caactcttca tttaatgcct tgtcatttga
    29821 tacataattt atctcttttt gcacaaattc tgctgctaat cttgcaattg tttgtttatc
    29881 catgcttccc cctttatagt tttaaattta tatgctccta ttatataata tttctcttct
    29941 tatgtgttac ttaatttcat attaaattct aacattctta taattgttta aaattattac
    30001 taacgggtaa catttattta attaaatgat agtgtgacta tatagaggag tgtctgctta
    30061 acactaccaa aaataacaag gagtacaaaa ctatgataac ttataataaa cacatcgagt
    30121 atctgcatga acttaagcgg aagattatgc tgcacagaca tagattgacg attatcatga
    30181 tgctggtcat cctgcttgga acaatggcat ccggctgcgc cataggagaa accgacacga
    30241 caagcccgaa cacaccagcc ggagagtcca ttgtggtaac gataaccgac ccgcccgacg
    30301 agttggagcc catcgttgtg ccgactatgc ctgaagttat tccaccctac ctcggtgctg
    30361 atccggagac cggtctaaca atgactggga caccgacatt ggttgatttc gacacctatc
    30421 ggctcaaggt gagcggtaag gtcgaccaag agctgagcct gacctatgat gatttgcggc
    30481 gcatgccaaa gctgactgct actccgacat tggaatgcgt tggatatttc aaggatgttg
    30541 ctacctggtc gggcgcatcg ctcaagacaa tcctcgagat ggcaaaagtg cagcccggga
    30601 gcaccagggt tgcctttaag ggcgctgacg gctacgaaat ttctatgatt ctggagaatg
    30661 ccctcgcccc cgagaatttc cttgcctacg aattagaggg taagacactt ccggtgctgc
    30721 aaggctttcc gttacgagta gtcatcccca gttatgacgg ctccgtatgg gtcaagtggt
    30781 tgcttgagat agtggtggag taaatataaa gtattaaatt caggaaggat taattatgaa
    30841 aaggcttttg attatactat ttatagtaat tacattaact ataacaatta caggatgtac
    30901 atcaatggaa aaaaatcaaa cgggtacaga aaccgcatat aaaaaaatat ctgctgaaga
    30961 ggcaaaagca ataattgaca gtgaagatgt tattatttta gatgttcgaa cacaggaaga
    31021 atacaatgaa ggtcacattg agaattctgt cttgctgccg gtaaatgata taacaggtaa
    31081 agcaggagaa atactggctg acaaggatgc aaaaatactt gtatattgca ggaccggaaa
    31141 cagaagtgcc acagcagcca aacaattaat caaaatgggt tatacaaatg tctatgattt
    31201 tggcggaata aattcctggc catatggaac agtgaaataa taaaaaaatg tcatcctgag
    31261 catatgcgaa ggatctcatg agattcttca ctgcgttcag aatgacactt acgtgtatta
    31321 ataacaagac aatgtgttgt atacttgttt ataaacttaa cgccttctta tacaaagaag
    31381 gcgtcttaat aagcttatgt ctatgcaatt aataactgat tgagtagtcg tgagaatact
    31441 ggagataaat attctggtgt gcaggaccaa atggtagggg aactgctccc tccgcacaat
    31501 tctgattctc gtttatacct tgtttcatat gggtcccata atcaattaca gagaagaact
    31561 taccatagtc gtcgtgggga ctgttagtat cctttggagg ataatagcgt atgagattgt
    31621 catatccaaa gtgtttaacc ctaagagcag taataggtct tcccttggtg cttgtcacat
    31681 aataaagctt taatctgtct gcgtaggaac tgatttcatt cttcagactg gggtcggatt
    31741 ttgcttctcc cccatcaccc caaagcttgg ccgttgggtc attgccccaa tattttttaa
    31801 atatttcctt ggcattttca ccctggcttt catttaccaa gaatactgca acaacaggcg
    31861 gccttgaagc caaatcgctg aaacctgttc taatatatat ttgttgttgt atttcatcgc
    31921 gataaaaatc gtgagcggtg atattagcta ctaaatacca tttatcattt gttatggggt
    31981 caataataac ttgttcgtcc aaaccgtcta aagttctgcc gacaggctgg ggattcaggc
    32041 gaataccatt tatacaggtt atggcggccg tgggaatata ttgattttct tccgcatatt
    32101 tttcataagc cttcttaagt tcataccttc taaaaggatt tctagccaaa ttataaattg
    32161 gtattaaggg tgtgtcttct ccaaaatcac acattgccgg gtattttcgc acatattctc
    32221 cccatgccgt atattgtgaa ggagtaatag tatttgagcc caggtaatca ggataagctt
    32281 taatattaat ctcacaattc tcgttaaaag aagtggtggt ttctttctgg cttgttgaat
    32341 gactggctcc ggctgaagca aaggccatat taaaagaagc ttttacttct gattcaaatg
    32401 tatgactccc gctgtgatag gaactgttgg cttgaacatt atagtctaaa cgacctccca
    32461 ttttaacagc ccttatcaca tgagtgccat atcggttaaa gatttcttcc ggcgtaacac
    32521 tatcatcttc tatatccttt ttgaatgaag agtcatagta tttagagtag tccaaatagt
    32581 tgtcatcaaa ttcaatcaga tactgagata aatcactgac taaggttgca tatctactgt
    32641 tagtttctgt gtaagtctga gagccgaaac tagcattgat acttccactg aaatacagat
    32701 aacctccgct tacgcctatt tttctggcca tttccttaga atatgaatat aaacttttac
    32761 cttcggtaaa gcggctgaca ctctgtcttc cgtcagtttt aaacactctc ttagcttcta
    32821 aaagcttatt agtactaagt acagagtctt ttaaactgta tgggtcagcg aattttccct
    32881 ttattacatc atatcccttg cctaaacagt ctactcccaa tacattgtca cttgctttaa
    32941 cccaatcaac tgatggttca taatcacttt cgtcgggaaa gtcatagtat tcatcccagt
    33001 atttctcaat ttcttccggt gttaagagtc gcagatctga acccggtttt tcttttaatg
    33061 agtcagtaat atccaaaccc ggtcctgtag gactgatcac aataccgctg gacaaggcag
    33121 tacctgcggt gaactgcgaa taggatgagg aacctgagtc attggccgga acaaaaggct
    33181 tgaatacatt cttattcaat gatggagctc tgccgtttaa taaacttgaa taatattcat
    33241 cgggatctgc ttgaccctgg gcttcaggtg cttccgaata cttggtaaag gtacgtttca
    33301 acaaaataat ggcctgctct cggttggtat tatttagagg tgatatggta gttgtgctgg
    33361 ttcccttcat aatatcattc ttgtaggcaa atttcattgc atcaagggcc caggaagcta
    33421 tgcttgacga atcctggaat ggaaaatcgc tgccaagact tgtgtcgata cccggtttgg
    33481 cagcctttaa tgcccttaat atcattacac aaatttcctg cctggtaata ttgtttgcag
    33541 gtgtaaaagt agtatcagat gttcctttta caatacccag ggcataggct tttaagattt
    33601 ccgcattatc agtgtccgta aaaggattgg ttcccattac gggggctttc ccccctaact
    33661 tttcatatag cttcacaaca ataacacaaa attcctcacg ggtaataggt ttttgataat
    33721 tattttctat ttgaccgtaa gtaagaccgt tttcatatgc ttctttaagc tctgcctctg
    33781 cccaggaact ctgagtgcca tcaaaattta tagactcggt ttcgaatgtt ggtataaagg
    33841 ataaggcaga agcaggtaag catataagta ccccaaacaa aactatcagt gaaatgcttc
    33901 tttgaaaaaa ttccgtccct ttcttcatac tttttccccc ttattaattg tgttaaataa
    33961 ttattgcgtt ttttccatat aatgtcaatc accctaaggg tgattattaa aaatttcata
    34021 aaaaaagcat caattaagat gctctaattt ataccctcca cagtctatgt taatatcctt
    34081 atttttttta tcctgtttct atcaatgttt tctattataa accttatatt ttcatactca
    34141 atttcttctc ctgcttcagg aagcctgtca ataataccga ttacaaaacc tgctattgta
    34201 tcaaagtcct cagactctat atgagttccg attaattcgt ttattatatc aattcttaca
    34261 ttaccgttaa ccagatattc atcttctttt attacttcga tttctttaat ttttttatca
    34321 tattcatctt ctatatcacc gactatttct tctactacat cctcgattgt cacaattcct
    34381 tctgttccgc catattcatc tataataaca accatatgat ttcggtttgt tctcatttcc
    34441 ttaaacagct ctttaatgga tttgtattcg ggagtgaaat atggttggcg tatatatttc
    34501 cttaaatcaa agggagattc tttattaaca tccaaaaata acaaatcttt tacatacaaa
    34561 attccaatta tattatcaat tgtgttttca tatacgggaa ttctggaata ttgctcttta
    34621 tttattattt ttataatttc ttcataaggc aaatcaacgt caatggcaac aatttctgtc
    34681 cttggaacca tcacatcatt aacttgtgaa tcactaaaat caaacacatt gtaaatcata
    34741 tccttttctt caccttctaa tactccttct ttgtaactta catttactat agtttttaat
    34801 tcttcttgtg taataaaggg ctttttttta tcaacttcgc cacccaataa tttaaccaaa
    34861 aaatcagtta taatatttaa cacaaaactg attggagtta aaagatttgt aacaaattct
    34921 ataaaaccgg atagtcttac cgatatcttt tctgaatttt tagctgctaa tgatttcgga
    34981 gttatttctc caaataccag gattaataag gtcattattc cggttgcgac acctacgccg
    35041 gtattgccga atgcatcgat agctaatgat gtcattaatg atgatgcacc gatattaacc
    35101 acgttgttac cgataagtat tgcgcttaac agcctgcttg ggtcactaag caatttattt
    35161 atcctatttg cacctttttc cctattttca atcatttgtt taacacgaat tttgcttaag
    35221 gacatcaaag ccgtctctga tgccgaaaat aaggcggata atactaaaca tataattaat
    35281 ataactattt tccatactct atccgaatcc aaaaccatcg ctcctaatac ataaaaaaca
    35341 taccttatct gtatttatgc tctgttttct ttgaaaattg ttttatatct attgttagtt
    35401 cttctttgtc tatatagtat tttcatctat gagaatttta acagaaatat tattttacat
    35461 cttttgataa tcaagtaata ttgtaataac caacctctgc gcctttgcat ttttgcttcc
    35521 aagggttttc taaaaggtat catactatag atataataca aaaagacgat tcattgattg
    35581 tttaaccaag aaatcgtctt tataacttta aaattgcttt tcattaatta ttcacattca
    35641 ccatgatgct ggtgctcatg acaaatatag cccgttgatt ttaaattacc tcttaaatat
    35701 tcttctgcgg caatttttgc ttcacctaga gcacccgtta ttacttcgat acctttttca
    35761 ttaaatatat caattgctcc ctggcccatt cctcctgata ttattacatt aacacctata
    35821 tcatttaaaa agtttggtag aaatcccggt ctgtgacccg gatttttaac tgactcgctt
    35881 tttactattt tatttccatc tgtttcataa atattaaaat tctcacaatg accgaaatgc
    35941 tccgtaacta tttccttttc acttgcaact gctattttca ttctaaccct ccatctcttc
    36001 taaaaattca gctataccgg taaatggttc atctccataa agctcaatca atcccttgtc
    36061 gcacatggat gatatttttg gatctatggg tagtttggca ataacattca aaccgtgttt
    36121 ctttgctaca tcttcaattt tactgtcgcc gaatattttg tattctttat tgcagtcagg
    36181 acatttgaaa tatgacatat tttcaactat tccaatgatt ggaatattca tcgattcagc
    36241 cattttcacc gctttctcca caatcattga caccaattcc tgaggagatg ttaatacaat
    36301 gattccatca accggtatgg attgaaatac agtaagtggc acatcacctg ttcccggggg
    36361 catgtcaatg aacataaaat ctatgtcttt ccaaattaca tctgtccaaa actgttttac
    36421 cgttcctgct attatggggc ctctccatac aacagggtcg gtagtgtttt ccaacagaag
    36481 attaattgac attacttcta tgctggtctt acttttcaca ggcagtaccc cattggaacc
    36541 accccttgct ttttcggtta taccaaataa ttttggtatt gaaggccctg taatatcagc
    36601 atccaatatt gcaacattat gatttcttct cttcatggta actgccagca aggatgttac
    36661 caatgatttg cctactccac ccttaccgct tataacacca atcacttttt tgatgttgct
    36721 ttgagggtgc ggcctttcca aaaaattagt ttgttcttct tttctgctgt cgcattcctt
    36781 actgcaggaa ctacagcttt cattacaatt ttcactcatt ttcttctcct aattgaataa
    36841 aattttcatt gtctttttat atatttttcg cacggcttca cctgccgcac aatcaatatc
    36901 aacaattgtt aatccattat ttacagcttc cacggctttt ttatcgaaag gaatttcccc
    36961 cacaaaatca attttattat cttgacaaaa tcgttttatc ttttcattgt ttgcaatatt
    37021 ggtatcatat ttatttacgc aaacggctaa ttttgtatta aatttttttg ctgtttctat
    37081 aattcttttc atatcgctga ttcccgacaa agaaggctcg gcaacaacca gtaccatagt
    37141 gacaccgctt attgatgcaa taacaggaca ccctatgccc ggcgagccgt caattattgc
    37201 aaaatcagca tccgctgctg caaacctcat gtttttcttt acttctgtta caagcatgcc
    37261 tgaattcccg cttcccatct ttaattcggc ggttgaaaaa acttgattgc ctcggtataa
    37321 tgtcatatct cctgctttat aaggcatcaa atttatggct gaagcaggac aaacagcttc
    37381 acagactccg catccttcac aatcatagag actgacagtg taatcactga ttgcatcaaa
    37441 tcggcagcta tcataacaca aattacacat taggcattta tccttatcaa tcactgcttt
    37501 atctaatccg taataatccg aattctccgg tatggattct tgttgcataa tcaaatgcag
    37561 attaggagca tctatgtcgc agtcagcgta agcttttaca tttgaaagtt tgataattgc
    37621 accggcaacg gttgtcttcc ctgtgccgcc tttaccgctt agaattaaca gctgcttcat
    37681 ttaacctcct cctttattgt ttttaaaaga gaggagaata gacttttata tttttcattt
    37741 ttttcagatg caatttctgc attcgaattt atattgccta attcatgctc gaaaggtatt
    37801 ttgcataaaa ctttaatatt tttttcatta caatattttt ccgccggatt ttctccgtct
    37861 aggcatttat ttaaaactac gccgtaaggt ttatcgaata atttaactaa attataaacc
    37921 atgttgaaat tatgtacgcc aaataaggtg ggctcagcca ccaatatgca gtaatcaaca
    37981 tcctttatgc tttccataac gctgcaggag cttcccggag gacagtcgat aaaggtatat
    38041 ggtttattgt cggtgatttt taacaatttc ttgattatgc ttactccggt ttcttcaccg
    38101 ggatttaaaa ttccggtata aatatttgaa ttattaaaaa cgccctcctg tatttctcct
    38161 atttctttat ctttttccga tatagctttt tcggggcata acagcatgca tcctccgcaa
    38221 gaatgacata tctcatcaaa tacatgtact ttgtctttta tatatgccaa agcattgaat
    38281 ttgcaaaaat ctacgcatct tctgcagcca ctgcaaagct cattgtttat gtttggaatt
    38341 ttaacgtgta ccttttcact tttaataata ttaggcttaa agaaaagatg tccgttaggt
    38401 tcttccacat cacaatcaat gtaatttgaa ttatcggcaa caaaagataa atttacagat
    38461 acaaatgttt tgcctgttcc gcccttgccg cttaaaacag caatcatcat tatttctctc
    38521 caccatgaaa acctgaatgt atttcagtca gtatttttag tttatcattt ttaaatgcgg
    38581 ttaaattttc ttcaattgaa tcatttaatg ctttataaat tttaacatta gctgctgtta
    38641 acacttctgc tgcattttgc ccgcatcttg gagttattaa tatatccgca tcattatcta
    38701 caataatctg agctgcttta atacctgcac cgccttgact gtttgccgca ctgttattaa
    38761 taaaactact atcattattt tctgtatcat aaagcataaa atatggtgtc ctgccaaatg
    38821 atacacatac atttccatca tctaccggaa ttgctatttt cattatttcc tccatattta
    38881 tgacatatgc caataataaa ttatatattt ataatcggca tatgtcaata tttatttttc
    38941 tttgccgtga aagcttgagt gaaattcatt taataaagaa agtttatttt cattaaaata
    39001 tgctatattt tcctctgcag tgcctttttg ggctgcataa ataaagacct cagcatttct
    39061 gaataatttt tctgcattct ctccacaccg atttgtaatt acagctttta caccattgtc
    39121 cgcaatcact tgtgcagctc taatacccgc acctccctgt tcaactactg ccctgttgtc
    39181 caaaaattcg tattcttttg taacagtgct gtagataagg aaatacggtg cgcgaccaag
    39241 ataatcatat atatttgatt taattgactt ttcttctgtt ggaattgcta ttttcatatt
    39301 atctccaacc tttctaatcg tcttttaaat cttccaattc tttttcaagt agctctattt
    39361 gtgattccaa gaattctttt tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39481 nnngacatat gtcatttatc ttatattata cactatatcc gacatatgtc aataactttt
    39541 ttaaaaaaac tgcaaagaaa tttttgcagt tttaattatt ttatatgaat acatgttagt
    39601 gtcttgcaat gattaacatt tttatcattg ctatgaataa gctatgtgtc attctgagag
    39661 cttaagctcg aagaatctca ttttttaaac cctgagatcc ttcgcttacg ctcaggatga
    39721 caaatggtat ttgttcctac agtttttcta attctcttac ataaacatct tcatatgtat
    39781 ctaagcctgt tacttgtgct gctaaatcag tcaatgggaa gatgtcgctt ctgtctatat
    39841 attttatgtc aaatttacgg ttcaaggcca ttaattgctt tattcctgca gctattctgc
    39901 ttatatatga atacactcct acggctcctg tcggtaagtc ttgtgcattg tcataataaa
    39961 gctttaattc tcttatgtca gcaaaaattt cttcctttgt gcttccgaaa ctttgatatt
    40021 ctttaggtat attgcctgct tcaatcaatt cgcccacttg ttttcctacc atggccgcag
    40081 ccattgcggc tcttccgaca gctacaaatt gaatatatgg agctcctaga gccaatccct
    40141 taaatatgtg gtcttctaaa gtaaatcctc ccgtaattgc aacttgaggc aagaaatatc
    40201 ctttcttatc cattcgtttc agtatatcat acagcatact ttccaaataa acggtaggca
    40261 ttccccattc attcatcatt ttaacggggc tgttccctgt gcctcctccg gcaccgtcaa
    40321 atgtaaccaa atccacttca ttttctgatg caatttttaa aatcctgatt aagtcttttg
    40381 ggtcgaaggg acctgtttta aagcaaatcc tttctgctcc caattctctt aaatgtgcaa
    40441 tgcggttttt gagaatttct tcattccata taggaagctt cccgattttt tcaaatatag
    40501 gtccttttcc atttttgaaa ttttcagcaa ctttttcgtc cgatgggtca ggatacacca
    40561 aatatccttt attttgaagt tttagagctt cctctaatga attagtcctt cccattcctt
    40621 gaattccttt tgcagcttgg ccgaatttaa gctccacgga ttttacgcct aattttggta
    40681 taacgtaatc taatactccg gaattctcat catcatagtt tgcctgcata atgatttccc
    40741 cataccctct ggagtatttc ctgaacatgt ctaccatttc tccaactaga ggagctgaaa
    40801 ctacttttcc gttttccaat actaaatttt tatcatttgg aatagcatct tcgccgatta
    40861 ccaccgatac tcctgcaaga gcagccccta caaagtaatc tttccaattt aattttgcaa
    40921 tggcaggcaa aattatcggc attttcattt ttactttgtt ttttatgcca aattcagttt
    40981 cagttttcac attcgtgtat gtagctttat tagcatcctc ttcacatccc aatgctccga
    41041 atactcttcc attaatagat agatgagaaa agtctaacgg ataatctttt tctgaagcaa
    41101 attgatttat atctctacga tatggaaaag ttgcttctga gcctctcact gccgataatc
    41161 ctatttcaca ggtaccgata caattagctg tgcatactga acacattccg gaatacggag
    41221 aaacgttttc cggcgtcctc atttttgtat tgcttatcgt acttcctaaa gcagggctat
    41281 aagtcataat aaacctccta attcattgtt tatattaatt atacctctat tgtgacatat
    41341 gtcaataata tatttgattt ttctatattt tatttatgct atctcaaagt taatgtgagc
    41401 actcagtttt ctagactatc attatgactc aagttaaata tttttctgtt atcttcttta
    41461 ttgtgccgtc tttttctaat tctttaaatc tcttattaaa caaatcaaga aggtgcttgt
    41521 tatttttagc tttaacaaac cctattcttg attcacttgt atccgggtga gcatatttgt
    41581 atttttcttc ctttactcta tcaacaagat ttaacttatt aaataagtaa tttccggaat
    41641 acgaattcaa aattactaca tctatttgcc ctttcaataa tttttgaaac attatttcct
    41701 ctttaaagct ataatttcta tcgaaggagg tgttttgatc aaattctgcg taatatttat
    41761 atcctgttaa tacccctaca cgtttaccgc ttaaatcatc tatattgcca atagaaatat
    41821 tattttcttt gcttgtataa aaattgtatc tgctttcatt tctataatta atagaaaaat
    41881 ccaaaaattc ttctctttct tcattaattt ctattgtagg aagaatatct actaaaccct
    41941 ttttggccat attaatagaa gtttcccatg gtactatttt atattcaact tgataacctg
    42001 cttctgaata gatttcatta atcatatcaa tatctattcc cgaaacaata tttttttcaa
    42061 tatcataaat tataaatggc tcataaggca tcgtagctat aataatcctg ttatcaggat
    42121 aataatcctt tactaattgt aaaaattcaa aatttacttc ttcgaattta ctgatactgc
    42181 tatttaaatt atctactgat ttagtttcta ataatactga ttgattaaca tcaacaattg
    42241 attttgttac ttcttcaact gaaagtgttg ttgaattaat gaattcttta agattatata
    42301 tttgttcaac aatcttttgt gaattaaatg atatatcact ggttttatta tatagctcct
    42361 tggatatgtt ttctatattt ttaaaactta caatggtttt atcaatactt atagtttgtt
    42421 cttcgatttt gtcaagaaca tctattaact cttttttagt attgacaaat gaacttctta
    42481 actcttttat tatctcagat ataacattag aaacttcatt agtttcagct gctaatgacc
    42541 taatctcaac tgctattaca gaaaaaccct taccatattc gccggctctt gaagcttcaa
    42601 tagatgcatt tattgctaat aaattagttt gctgtgaaat attatttatt ctacttatta
    42661 aggattccgc atttaaagtc ttcgtttcta aatctgcaat taaattgctt atattatcta
    42721 ataaacttct tactcttgat aattcatcaa tgttgtcttg aagctgcatt atttttttgt
    42781 atatttcttc atgagttttt tttgatattt cagatgaagt ttgtgaaccg gcaagattta
    42841 tttcaacatt attataaatg ttttctatta cttctgatat ggatatcata ctggctgtct
    42901 gttcttctga ttctgatatt atattttcaa ctttacctga tatttccttc atttccatat
    42961 atatttcctc aactgaaaat gagaacctgc ttatgagctc taaatatttg ccggataaat
    43021 cgttttcctt gttaataatt ttttctgcat taattggttg actaaataat cttttattat
    43081 ttttcataat tagtttataa atgattaaac cttcaattaa aataagcact ccaaatgcaa
    43141 ttaataaata taccataatt ttaattcctc caagattttt gaatccatat agaattttgc
    43201 attttattta tgtcttgcct agccccttgt aaagtaatta ttgatataaa tatacaataa
    43261 ttgactattt atgtcaatgt acttttcacg gattatcctt tatgtcacta acttgaaagt
    43321 tagttggctg tcactttcta cacaatagcc ttgaaataaa caatattgaa tgatataatt
    43381 tttgtattaa acaaattata atatagttat aaaccatata gaaaaactca ataatatgag
    43441 ctataaactt tggaggcaaa atgagttcca taacactaat atttacatcc ttaggattat
    43501 ccatggacgc ttgtgccgta tcaatatcaa acggtatgtg ctacggaaat atacataaaa
    43561 aacaaattat tacaactgcg gctgctttcg gtttctttca agcatttatg cctgttttag
    43621 gtttctttat aggctcagca ttcagtaatg ccatatcatt tttagaccat tggattgcac
    43681 tggttttact tggtttcata ggcgtaaaaa tgattgttga agcatttcgc gagcttaaat
    43741 atccggaagt atgcataaca ggtgcaaaac acctaacatc aaaaacattg ttctttcaag
    43801 ctttagcaac aagtatagat gcattagctg taggtatagg atttgccgtt atgaaagtga
    43861 acattttcag tgctgccctg ttcataggta taattacttt tgttaattct ataataggtt
    43921 ctaatttagg gaaaaaatgt gggcaattgt ttaaacaaaa agcagaaatt ctcggtggag
    43981 taatcttggt aggaatagga attaaaattt ttttaga
//
LOCUS       DAOPUE010000006        36596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1096_length_36596_cov_9.720506, whole genome shotgun sequence.
ACCESSION   DAOPUE010000006 DAOPUE010000000
VERSION     DAOPUE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 36596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36596
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_36596_cov_9.720506"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..834)
                     /gene="spoIIR"
                     /locus_tag="PKH42_01235"
     CDS             complement(157..834)
                     /gene="spoIIR"
                     /locus_tag="PKH42_01235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HOA19079.1"
                     /translation="MKYSKKVFISAVFVVLAIAIAYSAQVYAGLSEINGKVIRLHVLA
                     NSDSFEDQELKYKVRNKIITTFNKEFEDINYKSESSNIIVEKLNQIRCEAEEIVREEG
                     YDYDVNVYYGNYKFPRKAYEEIVLPEGYYDAVRIEIGKAEGSNWWCVMFPPLCFVDFG
                     RNQDDPILDFETEEKLQQVLTEEEIEVIKTKRGIENIKLKSKIFEFIEKGKVNKTPNT
                     YAASLSN"
     gene            complement(843..1940)
                     /locus_tag="PKH42_01240"
     CDS             complement(843..1940)
                     /locus_tag="PKH42_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOA19080.1"
                     /translation="MKKLILSLLIISTVFLFGCWDSVEINKREYLFAVGIDKEDDSLK
                     FISEIPKINEGSEEDRLVYTNEGKSFSDFYHLNYLHSDKVISDRLMQVIVLGEELARD
                     SDALKEIFDEIQRSPQMNRRVKICIAKGRAEDIINTEIPSNPIVGRYLSDMLVKLKRE
                     SYQDIFTFDEAILHLGQKGNAMIPVVEINEKSLKIERAAIVKEYELIGFLEPDELEAV
                     MLLLNPANANIRNINIEIDDTNISLGAVDVSMTDNIKLQGEKLQVDYYITLYCYIDSF
                     TIGNNNLEDRNFLNRVKDESIKKISETSTNAVDKLKNTYNADLLQIKDKLYKYHKRDY
                     EKIMDKYDEVFAKADINVYYKMEIKSVGLVK"
     gene            complement(2033..3109)
                     /locus_tag="PKH42_01245"
     CDS             complement(2033..3109)
                     /locus_tag="PKH42_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOA19081.1"
                     /translation="MFNQDITPYQNLSILLLVSFVLQSMLMPVILTGVDGPEGWISIL
                     IAAFILYFSIKTINKAMKKHDEDTIISVSDKLFSKFISKIIGLYYISMFITVNSLIIK
                     DFAEQIKLMMLFRTPISTIIITILLTASYATKKGIQTIANITNIALLTSLIPYLLIIV
                     FSTYYADYTNIFPVYPADAEGIIKSVPNAMLSFLGFSVLLFSNSRVDTKEKNMRINKR
                     YLVISAFLYIFSYILIIVKFGTKEASNLVWPFVSIMKFVNIPGFFFESTEIVGLCLQI
                     IVIFTSICILAFFTNLAMQETFKTNENGYFIFIQIPILYLMAAGLPGMYMMFPYIQMP
                     IYIISGMNLFIPMLVAYAGNRKKT"
     gene            complement(3102..4607)
                     /locus_tag="PKH42_01250"
     CDS             complement(3102..4607)
                     /locus_tag="PKH42_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOA19082.1"
                     /translation="MKDIDLTQKIDLDLNKNEQILRKIFENTDDIIFRKLEGGKIKKL
                     KMLLFYVDGMTSKEAISEYAISSLLEGLDLEKIQNMSNVQLQDAIVRTSISISEIQIL
                     TTIEECVDKILSGETVLLLENCNKGIMLSSRGWPMRGVQEPSAETLIRGARDGFNETM
                     KVNISLIRRRIRDPKLKVKYTQVGNRSKTDIAILYIEDLVNNTVLETVEKRINNIEID
                     AVLESSYIEEMIEDDGYSPFPQIENTERPDAAASALLEGRVVIAIDNTPSVLIAPAIF
                     VSFMQSSEDYYERWLPACATRILRYFALPIVMLLPALYVAVTQYHPNLLPTQLALYVA
                     ASRANVPFPPYFEALLMELVIELVREASFRITTPVGSTIGLVGGLVIGQSSVEAGLIT
                     PLAVIVVALTAIASFTIPSYNFGATLRLIRFGFIILAATFGLFGISIGLSFLIIHLCT
                     LKSCGIPYMTPFTRFVENKRDLKDTIVRPRISKMVRKPDYLHMEKKERKNV"
     gene            complement(4616..4816)
                     /locus_tag="PKH42_01255"
     CDS             complement(4616..4816)
                     /locus_tag="PKH42_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19083.1"
                     /translation="MFFINDLKRIINNDVIIVFLIISYILIFVTSKELKRNNYHRDYK
                     IVRFTGIIYGLLAIAAATAIYW"
     gene            complement(4965..6644)
                     /locus_tag="PKH42_01260"
     CDS             complement(4965..6644)
                     /locus_tag="PKH42_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA19084.1"
                     /translation="MSSIKNSLDLGFIITDDKFNVLYCNEYFNTNIIKTDILNKKITM
                     FFQDINVDMDKNLYSVKCKDNSVYYVFHNKFKIYNHELYSFFFYDFTIGSELQKQIER
                     LNSQQYIYNEMLNKLQDGIYITDERGTTIYANDAFLNLSGLTREQIIGNTVYDLRKSG
                     ILPNSCCAKVIETLEPVSTINHYYKGQRCLVSGSPIFDENGNLSKTIAVIRDVTELDM
                     LMKTIAKEKNLFIKSDYNVKTEAARDPDEIINNVKMKNIYEKAKKISKVDSTVLILGE
                     TGVGKDFIATYIHSISNRSKGKFIKINCGAVPEHLLESEFFGYEEGAFTGALKGGKKG
                     LFEEANGGILYLDEIGDMPYTLQVKLLSAINDKMFYRIGGTSPIEFNARIIAATNHDL
                     KQLVEEKKFRADLYYRLNVVTLVIPPLRNRREDIIPLSTQFLEYYNNKYGKNCYITSS
                     CLQSFLIYEWPGNIRELKNIIERLVLMAEGAEIGTELFRDNLILPDSIELTELSELFI
                     TGSKTLKEKMDEYEKEIIESTLAVSKNMKEASMRLGIDISTLVRKKQKYNL"
     gene            6937..8115
                     /locus_tag="PKH42_01265"
     CDS             6937..8115
                     /locus_tag="PKH42_01265"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOA19085.1"
                     /translation="MKKAVIVSAARTPIGSFGGAFASLSAVDLGVIAAKEAIKRAGIE
                     PSMVEEVYLGNILGAGLGQNVARQVSLGAGIPIETPALTINMVCGSGLRAVSMAAQFV
                     ESGQCDIILCGGTESMTDTPYLLPKARWGHRMGDGKVVDYMVHDGLWDIYNDYHMGMT
                     AENVAEQYGITREEQDEFAAQSQNRAEEAQKSGKFADEIVPVIIPQKKGDPVVIDTDE
                     YPRHGSTVEKMSKLKPAFKKDGTVTAGNASGINDGAAALIVMSKDKAGELGLKYLCEI
                     AGYGSAGVDPKVMGLGPVPATKKALANAGWTIDDLDLIEANEAFAAQALAVIRDLGLN
                     PEITNVNGGAIALGHPIGASGARILVTLLYEMEKRDAKRGLATLCIGGGMGTSLLVQR
                     "
     gene            8183..8836
                     /locus_tag="PKH42_01270"
     CDS             8183..8836
                     /locus_tag="PKH42_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOA19086.1"
                     /translation="MNKIISIDEAANMVKEGMTIMVGGFLANGTPETLIDALVEKNVK
                     NLTLICNDTGFPDKGVGKMVVNKQFKKMIVSHVGTNPETANQMNSGETEVVLTPQGTL
                     AEKVRCGGNGLGGFYTPTGIGTEVAEGKEIRVINGKEYMFETPLRADVALLRASVADK
                     KGNMIFNKTMKNFNPIMATAADLVIVEADKIVEVGEIDPEHVMCSGIFVDYIVGGDK"
     gene            8836..9495
                     /locus_tag="PKH42_01275"
     CDS             8836..9495
                     /locus_tag="PKH42_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOA19087.1"
                     /translation="MDAKNVIAKRVAELLHDGDVVNLGIGLPTMVANYIPEGMDITFH
                     SENGFLGLGPCPKEGEEDWELVNAGGMPSSIVPGGMFFDSATSFSIIRGGHVDATVLG
                     AMEVDEKGNLANWKIPGKMVPGMGGAMDLVVGAKTVIIAMVHTQKGKPKILKQCTLPL
                     TAKEQVDIIVTEMALIRVTKDGLVLEELGPEATIEDVVAATEANLIISPNLKRFGVEE
                     E"
     gene            complement(9633..10889)
                     /locus_tag="PKH42_01280"
     CDS             complement(9633..10889)
                     /locus_tag="PKH42_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOA19088.1"
                     /translation="MDFENNFNDNLEKENQNNITNDSMSNEYGEINFVMNEPLIKKPK
                     KRRIKSVMGYIALVLVAAIIGGLTGSYVTANMLNAAAEKKAAESSINAQEVKINLTDD
                     VYFAVAVAEKSQKTVVGITTEVERQINTFWGPQKRTEQHMGSGFIVDSNGYIVTNSHV
                     IGDGNYKNITVSLIDGSIETGNVLWYDTNLDLAIVKINKTRLPAAELGNSDELMVGEP
                     AVAIGNPMTLDLERTVTQGIISGLNRSIQFEDGTVIEPLIQTDASINSGNSGGPLFNA
                     KGQVIGINTAKMTSAEGLGFAIPINIIKPVLTQVVTGGTFIPVYVGITGMDIETYEML
                     YGVDLMADYGVIIIETMKDSPAREAGLQPNDVIIEIDGKKTEYMVDIQKKLYEYQDGD
                     KSEFKILRNGEELKLVLTLRNRPDGI"
     gene            11115..11492
                     /locus_tag="PKH42_01285"
     CDS             11115..11492
                     /locus_tag="PKH42_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOA19089.1"
                     /translation="MKKLFKIVIQIAIIYAIYQAGNFISSLISNIIVIPGNIIGMVIL
                     LVLLITNVLKFSIIEETSNFMLKYMSFFFVPITVGIMESYKLIQDSIVQIIIILFISC
                     ILVMYVSCKVTDVLISYREKSHD"
     gene            11485..12162
                     /locus_tag="PKH42_01290"
     CDS             11485..12162
                     /locus_tag="PKH42_01290"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOA19090.1"
                     /translation="MIDILTALFFILLTIIVFKFSLLVNDKFKIVFLNPILLTAFIII
                     VILKIFNFTYSDYYKGGKILVSALGPIVVVLAIPLYKNRHDLVKNFIPIVGGIISGII
                     TTFVSITVLCKLFGIDEIIFLSLLSKSVTTPMAVESTKILGGNEAVTILAVIITGLIG
                     AAFAPIVINKGKIKNNIAKGIGIGSAAHALGTSKAIEMNEEAGAASGLAIGLTGLLTI
                     IAVIIFA"
     gene            12172..12705
                     /locus_tag="PKH42_01295"
     CDS             12172..12705
                     /locus_tag="PKH42_01295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOA19091.1"
                     /translation="MINLSEIKKEDMDSIYKWFADTEFLKYYDYYPPNPLDKSDVDKM
                     FKYYEESGKSKVFAVRKESTIIGVAGFDDIIKENQVATLFIGLGSENERGKGYGTETM
                     RLLLKYGFENLNFHRIQLNVLEFNDKAIALYEKCGFKKEGIFREFVLRDGKRYNLLLY
                     GLLKSEWEKTEILRLRS"
     gene            complement(12884..13213)
                     /gene="trxA"
                     /locus_tag="PKH42_01300"
     CDS             complement(12884..13213)
                     /gene="trxA"
                     /locus_tag="PKH42_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA19092.1"
                     /translation="MAENIKIINSTQEFDDMIKQNRYVLAEFWAEWCTPCRMVAPVIE
                     KVAEQYSGKINVAKVDIDEQQELAIRYGIQTIPTIIFFKEGKFASKEIGVKPLASFAR
                     MIDSNIA"
     gene            complement(13197..14072)
                     /locus_tag="PKH42_01305"
     CDS             complement(13197..14072)
                     /locus_tag="PKH42_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOA19093.1"
                     /translation="MGDIVDIIIIGAGPAGLSAAVNAVTRGKTVRIFSNKENNLSKAE
                     RVDNHLGFYHASGKELMDKFKEHAKAMDIKIEEGMAVNILPLGESFMVNVNGEIFESK
                     KVILAMGITKVKELPGEGRLLGNGVSYCATCDGMLYRGKNALVWDQSKDAWEEANFLQ
                     SIGVNVTFISKKPKQENLSNDIKFIEGTITEIIGENTVEAVNTGNDLIKTDAVFMLRD
                     AVAPAALIDGLELDGNFINTNKHMETNIPGLYAAGDITGKPLQLSKAIGEGLIAAQHA
                     ALQIDKKENLNGRKY"
     gene            complement(14223..14948)
                     /locus_tag="PKH42_01310"
     CDS             complement(14223..14948)
                     /locus_tag="PKH42_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOA19094.1"
                     /translation="MGGAGLSTNVSIILVFAEGFLSFFSPCVLPLIPVYISYLAGNGR
                     KISSDGTITFERKKVLFNTLFFILGISSVFFILGLSFSTLGIFFNNNRELFSKIGGII
                     IVIMGFVQLNIIKLNFLKVEKRINLNIGGKINPILAYIMGFTFSFAWTPCVGPALASV
                     LILASNATNAFAGNMLVFVYALGFIIPFIILGVFTTEALNFFKEKRQLVKYSIKIGGI
                     ILIIIGIMTFTGIFTAISRNLSF"
     gene            complement(15118..17949)
                     /locus_tag="PKH42_01315"
     CDS             complement(15118..17949)
                     /locus_tag="PKH42_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOA19095.1"
                     /translation="MRNKGRLFVLGIVVIVFLIINSFTNIVEFITNYLWFKEVGYTQT
                     FFTKIKAQIMIGIPIFIILSILLYIFIKKLKKKYDDETEIIVVNKKFNLTIKLISAGA
                     SLLITLNIISTMWFEILQYINGVEFGATDPIFNNDVSFYVFKLPLINTIVSSIISILF
                     LLTVIIILFNGFLALKDSIKNVSEKFEDIQQFPRKQIDLNNILNKKFVERIINQLSII
                     GVFLFLLLAVRYVLRSYDLLFSRLGRVFGAGYTDINVTLNLYRILAVGCVLAAITFFI
                     GARKRNLKTALAVPAALIIISIAGTGVAGAVEKFVVEPDQLSKETKYMQYSIKSTQNA
                     YSLNNVKTIQFPANNNLTIEDLQNNPEVIDNIRINDQEPLIQVYNQLQGIRPYYVFND
                     VDVDRYVIDGDYKQVFLSARELDQDRLNEQARTWVNQYLKYTHGFGITVSTVNNVTPQ
                     GQPEILVKNIPPTTETDFNIQRPEIYFGEKTNNYVIVNTDEMEFDYPSGADNVETIYE
                     GKAGINLSFFNRLMFSIRKGSYRMMISNNIDSNSKILINRNIMQRVTEIAPFMYYDPD
                     AYIVVNQDDGKLYWIIEGFTVSNRFPYSQPTDIFIKGMSVNYIRNSVKVVIDAYDGTI
                     DFYVADENDPIIKTYDKIFTDLLKPIDEMPEGIRKHIRYSRAFFDVQSDMYRLYHIEN
                     VTVFFGREDYWDLANEKYMGGGEVPAGSSYLMFKLPGEEDVEFLLTNQYTPQNKDNMI
                     ALLAARNDGENYGELVLYEFPKTKVIPGPNMIETKIDQDTNISSQLTLWSQLGSDVLR
                     GNTLVIPIEESLLYVEPIYLKSDTDSNFPEMKMVVVSYGDKILMEPTLDTAINRLFGI
                     TEQEPGRPQVPDEEYDDTNINDLIIKANEVFNDANEASQNGNWAEYGRKINELERLLN
                     QLNALINGQQEQEARDENVQEESSGMPSE"
     gene            complement(17961..18722)
                     /locus_tag="PKH42_01320"
     CDS             complement(17961..18722)
                     /locus_tag="PKH42_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HOA19096.1"
                     /translation="MIEPSKNSKRIKNYDLFLLYHETKDIEIRNQIFEKYKYMAEIIS
                     RKYSNRGIEHDDIYQIACMGLIFAIERFDISKGFEFTSFATPTILGEIKKYFRDKGWA
                     VKVPRKIQEISRKVNEYNNILTARLHRAPTIKEIAEYISSTEEEVLEAFEAGRMFNSQ
                     SLDEKFDLSRDDNDISLMDVVGAEDKHYNLIENLDFLNKSMSKLNELEKNIIIKRFYE
                     NKTQSDIAKELKISQMTVSRIEKKSLEKLRIEYYR"
     gene            complement(18724..19098)
                     /locus_tag="PKH42_01325"
     CDS             complement(18724..19098)
                     /locus_tag="PKH42_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19097.1"
                     /translation="MDKILLKIPKKSEYMSTIRLTSSSLFNLNGFNIDEIEDIKVIIS
                     EICTFFIMNLSRSTEDFEISYQVFKNKIIVTVADLNDGELSEESLNNDMSILIIESLS
                     DNFNYDLNNKTITFEKNKIGVD"
     gene            complement(19103..19414)
                     /locus_tag="PKH42_01330"
     CDS             complement(19103..19414)
                     /locus_tag="PKH42_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOA19098.1"
                     /translation="MSLEIESKIQDSIIEIKPIGEVDIYTSPELKNQIFSLIEERNTS
                     IIIDGESLEYIDSTGLGVLMSIYKKMQENSLNLEIINLKPNIYKLFDITGLNKVFNIR
                     E"
     gene            complement(19443..21881)
                     /gene="gyrA"
                     /locus_tag="PKH42_01335"
     CDS             complement(19443..21881)
                     /gene="gyrA"
                     /locus_tag="PKH42_01335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOA19099.1"
                     /translation="MSENVNKSKIVNINISEEMKRSYLDYAMTVIVSRALPDVRDGLK
                     PVHRRILYSASELGLSPDKPHKKSARIVGDVLGKYHPHGDTAVYDAMVRLAQSFSMRY
                     PLIDGHGNFGSIDGDEAAAMRYTEARMSKIAMEMLRDFNKDTVDFRPNFDETLKEPLV
                     LPSRFPNLLVNGSSGIAVGMASSIPPHNIGEVINGIIAYIENPDIDIPGLMRYIKGPD
                     FPTGAIIEGKDNIKNAYLTGRGILKVKAKAEIEELKNNKSQILVSELPYQVNKARLIE
                     KIADLVKDRIIEGISDIRDESDRDGMRIVIEIKRDYNPNIVLNQLFKHTQMQDSFSVI
                     MIALVNNEPKVLNLKEMIYHYVEHQREVIVRRTKYDLDKAEARAHIVEGLLIAIDNID
                     EVIKIIRASYDDAEKRLMEAFNLTEIQARAIVDMRLRRLQGLEREKLNAEYEDLIKMI
                     NKFKEILSNQNLIDQIIKDELKEIKNSFYDERKTKITADEGEINVEDLIEEENVAITL
                     TNFGYIKRLPEDTYKVQNRGGKGITALTTRDEDFVKDLFITSTHDYLMFVTNKGKIYK
                     IKAYEVPEARRQAKGTAAINLINIEPGEKIKSLIPIREFNDNEFLIFVTKKGIIKKTK
                     LSEFESNRKTGLVAIKIAEDDELISVNKTDGTKDIFIVTKQGKGIRFHESDVRETGRN
                     TMGVKAITLSKNDELVAMSIIRKSDENKHILTVTENGYAKITLSQEYRPQSRGGKGMK
                     AHNINKKTGLIVNSLIVDKEDDIMILSTSGTIIRMHARDISIMGRDTQGVIAMRLKGD
                     DKVASIAKIFSEED"
     gene            complement(21897..23810)
                     /gene="gyrB"
                     /locus_tag="PKH42_01340"
     CDS             complement(21897..23810)
                     /gene="gyrB"
                     /locus_tag="PKH42_01340"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOA19100.1"
                     /translation="MATDKNNNHYSADQIQVLEGLEPVRKRPGMYIGSTGERGLHQLV
                     YEVVDNSIDEAMAGYCDTIKLTINEDNSVVVVDNGRGIPIETNKKTGKSTLETVLTVL
                     HAGGKFDNDAYKVSGGLHGVGVSCVNALSEYLVAEVNWDGKKYRQRYERGIAKTEVEF
                     IGNTNKTGTSITFAPDPTIFDTTVFNYTTIKHRMRELAFLNKGIKIILEDKRQKIKNE
                     FHYEGGIREFVQFLNSKKEPLHNDVIYTEGTKDKVNVEIAIQYTDSYNENIFSFVNNI
                     NTIEGGTHLVGFKTALTRVINDYAKKANLIKENEVGITGEDIREGITAILSIKITNPQ
                     FEGQTKTKLGNSETRGIVDSITGEALSIYCEENPKTAKAIVEKALKSARAREAARKAR
                     DLVRRKGALDSMALPGKLADCSEKDPALCEICIVEGNSAGGSAKEGRDRRTQAILPLR
                     GKILNVEKSRIDRILNSEEIKNMITAFGCGIGEDFDISKLRYHKIIIMTDADVDGAHI
                     RTLLLTFFYRYMKELITGGYVYAAQPPLYRVRKGKFEKYVYSDDELNQVLDEIGRDNK
                     NEIQRYKGLGEMDPEQLWETTMNPENRILLRINEDDAIEADEIFNVLMGDKVAPRREF
                     IEENAKYVQNLDI"
     gene            complement(23822..24937)
                     /gene="recF"
                     /locus_tag="PKH42_01345"
     CDS             complement(23822..24937)
                     /gene="recF"
                     /locus_tag="PKH42_01345"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOA19101.1"
                     /translation="MIVKKLIIKNYRNFKEAEAELNNSLNIFIGDNGQGKTNLMEAIY
                     IASIGRTFRLNSENELINFFENKSSVEIYIEKNNYKIKIEILLEKNKKKQVIINGVKL
                     EKTSEMIGILNNVIFTPDDMKIIKGSPVERRRFINIDISQIKPKYKYLLNKYKKVTTE
                     RNLLLKNYYTKCENKDIISIWNDYLVNTGTEISLYRNEYINKLKKAATDIYSSISGNK
                     EKLDILYLSNIGCITDRDEVKKIFYDKINKNFNNEILKGISLYGPHKDDLIIKINGKE
                     CKYFGSQGQQRSVILALKLAEIEIIKEETGEYPILLLDDVLSELDNKRKSYLIEHIKD
                     IQTIITSTDEHELNILTKNFDKKMFYINEGKISNISN"
     gene            complement(24984..25196)
                     /locus_tag="PKH42_01350"
     CDS             complement(24984..25196)
                     /locus_tag="PKH42_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOA19102.1"
                     /translation="MEKININTEYIKLDQLLKYTNAVEGGGMAKNVILDGLVKVNGEV
                     VLQRGKKLREGDIIEFNNEKYLIERY"
     gene            complement(25285..26394)
                     /gene="dnaN"
                     /locus_tag="PKH42_01355"
     CDS             complement(25285..26394)
                     /gene="dnaN"
                     /locus_tag="PKH42_01355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOA19103.1"
                     /translation="MKLKINQNELNKAINIVQKAVSSRTPLPILSGILIEAKDNILTL
                     TATDLELGIKTYFPCEIEEEGSIVVQSRLIGDFVRKLPSNTYVFIETMGNNNMEIKCL
                     NSEINILGNSADEYPNNTFDNEGETFKIKSEVLKNLIKHTYFAAAIENIKPLFTGCLI
                     EIKNNLCTVVALDGYRMAVKKEKIDYKGDISIVIPSKTLFEILRIIEENKDETEITVS
                     ESHISFKVENTIIISNLLEGKFIDYEGIIKDNYVTVVKVNTEDMRDSIERASLLARDD
                     KNNLIIMDIKDFNMQINSASEYGNVEENISIDKEGEDIKIGFNSKYILDYLKVIDSEM
                     ISLNLIGKNNPCFIKEENEENKDNYIYMVLPVRIS"
     gene            complement(26636..27970)
                     /gene="dnaA"
                     /locus_tag="PKH42_01360"
     CDS             complement(26636..27970)
                     /gene="dnaA"
                     /locus_tag="PKH42_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOA19104.1"
                     /translation="MNFEELWNEVLEIVKEDTNQVSFNTWFKPLKIVAYKNNTIYLET
                     ADDFLRNTIKKRHYNFLKNAFNYVLKKEIDIIFTIPGENIDKEESKKYNTNSLDDTDA
                     IYNGRILNPKYRFDNFIIGNSNRFAHAASLAVAEAPSTAYNPLFIYGGVGLGKTHLMH
                     AIGHYILDNNPDTYVLYVTSEKFTNDLINSIKDGTNEEFRNTYRKADVLLVDDIQFIA
                     GKESTQEEFFHTFNALHEANKQIIVSSDNPPSEIPTLEDRLRSRFEWGLIADIQPPDY
                     ETRIAILRKKAEAENYNIPDEVITYIAQHIQSNIRKLEGALIRIYAYASLTNKNEVSL
                     ELAQEALKHLISKNKKVSASDIKEVVANYFNISIDEIESKKKTKNIAYPRQIAMYIAR
                     KLTDLSLPKIGEEFGGRDHSTVLHAFNKVEEDMENSQEVKKNIEDLISMLNN"
     gene            28427..28561
                     /gene="rpmH"
                     /locus_tag="PKH42_01365"
     CDS             28427..28561
                     /gene="rpmH"
                     /locus_tag="PKH42_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOA19105.1"
                     /translation="MKRTYQPKRKQRSSEHGFLKRMSTKSGRNVIKRRRARGRKKLSA
                     "
     gene            28611..28946
                     /gene="rnpA"
                     /locus_tag="PKH42_01370"
     CDS             28611..28946
                     /gene="rnpA"
                     /locus_tag="PKH42_01370"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOA19106.1"
                     /translation="MIIIKKNNDYKAVYNCNNSISDNNLVLFIKKNNYNYNRFGFTTA
                     KKIKKAVHRNVIRRRLKEIVRANEHYLEEGYDFVLMARINAVDSDYKSLESSFIKLMK
                     RKKIYRYTI"
     gene            28943..29152
                     /gene="yidD"
                     /locus_tag="PKH42_01375"
     CDS             28943..29152
                     /gene="yidD"
                     /locus_tag="PKH42_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOA19107.1"
                     /translation="MSKVLIFIIRIYQKFSSKSAVRHCRFYPTCSQYFVEALQKYGFL
                     KGSYLGIRRILKCHPFNPGGYDPLK"
     gene            29163..30044
                     /locus_tag="PKH42_01380"
     CDS             29163..30044
                     /locus_tag="PKH42_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOA19108.1"
                     /translation="MNLDFIAVPMGMLVKLVYNMVSVVDSKYLSAYSIAIIISTILFK
                     LIVLPLTLKQSKSMKKMQELSPKIQELQQKYGKDPQTLQRKQMELYKEANYSPFSGCL
                     PLLIQFPILIAFFYVIREPVKYVFGDQAFYDSINRAFLWIKDLNVNENTVWFLKNNDW
                     IVLTNAIKEEFSISSSMHLNQVMEVLNAQDIMVNGLSMGGMTLPFLGSALPVLAVITG
                     IITYLTTKMTAQPSINEQQASTQKTMNMMMPIMIFVFSIQFPAGLALYWVISNLFQLV
                     QQFIVLNSSKNPKEELK"
     gene            30045..30668
                     /gene="jag"
                     /locus_tag="PKH42_01385"
     CDS             30045..30668
                     /gene="jag"
                     /locus_tag="PKH42_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOA19109.1"
                     /translation="MRNVTIEKPTVEEAIAAALKELRAENNEVEIEIINEPSKGFFGL
                     GSKDAKVKVTITNGPEERARKFIDILLKKMSIEADYEVNYSDNILNVDIVKIGEDDKG
                     IVIGKRGKNLDEIQFLLNLMVNKGRENYVRVIFNVEDYRAKREETLKRLALKMADKCR
                     YYNRKVKLEPMNPYERRIIHSTLQDVKGIITYSEGEEPYRKVVIDQK"
     gene            30816..32189
                     /gene="mnmE"
                     /locus_tag="PKH42_01390"
     CDS             30816..32189
                     /gene="mnmE"
                     /locus_tag="PKH42_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOA19110.1"
                     /translation="MFSDTIAAIATFPGNAGINIIRLSGNDALNIAYKIFVDKNKRKP
                     DNLKPRYLHYGFIVDKDKKVIDEVLLSYMKAPNTYTREDIIEINCHGGIISAKKILDT
                     VLHYGCRTAERGEFTKRAFLNGRIDLTQAEAVIDIINSKTESSHEISIKHLEGRLSKQ
                     INAVIDEIMELLANIEVNIDFPEYDEDEITIEKVKALCHNLVPELDKLINTADTGKII
                     KEGIKTVILGKPNVGKSSLMNFLLNENRAIVTEVPGTTRDTIEEYVNIKGVPLRIIDT
                     AGIRETDDMVERIGVEKALEKVKEADLVIMIFDSSKELEKEDESILDYIKDKKVIYIR
                     NKTDLESRLDLSSYKKIEEEIINVSILQNQGLDDIINKIYSLFFKGRLDIRDELIINN
                     VRHKNLLIKAKNNIEEVLKSIENNMPIDFIEIDLMQAMENLGLIVGKSVSDDLIDKIF
                     DEFCIGK"
     gene            32203..34092
                     /gene="mnmG"
                     /locus_tag="PKH42_01395"
     CDS             32203..34092
                     /gene="mnmG"
                     /locus_tag="PKH42_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOA19111.1"
                     /translation="MEDRVREFLEESIDVLVIGAGHAGCEASLATARLGLKTIMLTLS
                     LDSIAMMPCNPNIGGTAKGHLVREIDALGGEMALNIDKTMIQCKMLNRSKGPAVHSLR
                     AQADKKKYHREMKKVIENQKNLRVIQTEATKIKVNEDKSFNVYTKLGAYYRARAVIVT
                     TGTYLKGRIYMGELNYSSGPDGLAPANELSDSLMDLGIELIKFKTGTPARIHKDSINY
                     EKMTIQTGDDEIIPFSFMNDEINIEQVPCYITYTTQQTHELIRNNLDRSPLYGGVIKS
                     VGPRYCPSLEDKVVKFSDKEKHQLFVEPEGLDTKEMYIQGMSTSLPYEVQIQMYRTMI
                     GLENAEIMRPAYAIEYDAIDPTQLKPSLELISIKNLFFAGQINGSSGYEEAAAQGLIA
                     GINAYLSLNNKEPFILDRSEAYIGVLIDDLVTKGTTEPYRMMTSRAEYRLLLRQDNAD
                     ERLTEKGYKIGLVTEERYKRFKDKRQRIENEINRLKNLKITPKADTNKALEDMGSAAL
                     KIPTTLYDLLRRTELNYDNTLFLDPKRPVLRKEIKDYVETVIKYEGYIQKQIAQVEQF
                     KKLENKKIPEDINYDDVKSLRIEARQKLNKIRPNSIGQASRISGVSPADINVLLIYLM
                     TYNKE"
     gene            34103..34816
                     /gene="rsmG"
                     /locus_tag="PKH42_01400"
     CDS             34103..34816
                     /gene="rsmG"
                     /locus_tag="PKH42_01400"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOA19112.1"
                     /translation="MIKTLEEGFKELKLPYSKDIENNFIIYRDLLKEWNKKINITSIE
                     NDGEIYIKHFIDSVLLLNENNINENKTIIDVGTGGGFPGLPLKIVNNNYKITLLDSLR
                     KRIDFLSEVVKALNLKDVKLIHGRAEDYGQNKEYRECYDICVSRAVAPLNVLSEYCIP
                     FVKVGGYFAAYKSDNISQEISSSDNAIKKLGGEIKEIKKINLPGTDIIRKIVIIEKTD
                     KTEIKYPRKAGKPAKDPLK"
     gene            34827..35615
                     /gene="noc"
                     /locus_tag="PKH42_01405"
     CDS             34827..35615
                     /gene="noc"
                     /locus_tag="PKH42_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOA19113.1"
                     /translation="MISNIVHIDVNKIIPNKNQPRKTFDEKALKELSQSIDNYGIIQP
                     ITVRKIYDDIYEIVAGERRYKAVKMLNMDTIPAVIIEAKEEESAAMSLIENLQREDLH
                     YLEEAYAYERLINEFGISQTQLAQKLGKSQSAIANKVRILKLPESVKEKLREGNLTER
                     HGRALLKINDEEILLNIVEKVIKKDLNVSETEKIVDSIADELNIKKKADKRYVRNFIN
                     YKIYINTIKNAYNEIVKTGIDAKFEQNESDDYIEIKVKIPKKSM"
     gene            35627..36388
                     /locus_tag="PKH42_01410"
     CDS             35627..36388
                     /locus_tag="PKH42_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19114.1"
                     /translation="MTKVISIFNQKGGVGKTTTNVNLCTALALKGKKVLSIDIDPQGN
                     TTSGFGFNKNKLEYTIYNILIEDYDINKTIFKTEIENLDIIPANIQLAGAEVELINTK
                     FREKTLREKINLIENKYDFIMVDCPPSLGLLSLNALTASNTVLIPIQCEYYSLEGVGQ
                     LIDTVKLVKKNLNPKLEIEGVILNMFDGRTNLSIQVVEEVKKYFKDKVYRTVIPRSIR
                     LAEAPSFGQPIMLYDDKSKGSIAYIKLSEEFIKNN"
     gene            36403..>36596
                     /locus_tag="PKH42_01415"
     CDS             36403..>36596
                     /locus_tag="PKH42_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOA19115.1"
                     /translation="MAAKRKALGKGLSALIPEDLEKEDYEKIHEIDIDLISPNPNQPR
                     KLFEQDKLDELTESIKKYGV"
BASE COUNT        11738 a         6298 c         5914 g        12646 t
ORIGIN      
        1 aatataaaat caaaaaatta gggcttctct tttaggtgac aatggggacg tacctcgctg
       61 tccctggtat ctgttggact taagtcaaaa aatcattcta aaataaccgt cctctttaac
      121 cttggaatgg gacagcgagg aacgtcccca ctgtctctaa tttgataggg aagctgcata
      181 agtgtttggg gttttattta ctttgccttt ttcaataaat tcaaatattt ttgattttaa
      241 ttttatattt tcaattcctc ttttagtttt aataacttcg atttcttctt cggtaagtac
      301 ttgttgtagc ttttcttcag tttcaaaatc caatataggg tcatcttgat ttctcccgaa
      361 atctacaaag cacaggggcg gaaacataac acaccaccaa ttgctgcctt cagcctttcc
      421 tatttcaatc cttactgcat cataataccc ctccggcaag actatttctt cgtaagcttt
      481 gcgaggaaat ttataattgc cgtaatatac atttacatca taatcataac cttcttctct
      541 tacgatttct tctgcttcac accttatttg attaagcttt tcaacaatga tatttgaact
      601 ttcagactta tagtttatgt cttcaaattc cttattgaat gtggtaatta ttttgtttct
      661 cactttatat ttcagctctt ggtcttcaaa gctgtccgaa tttgccaaaa catgcagccg
      721 tataacctta ccgtttattt cggacaagcc tgcataaact tgtgccgaat atgcgattgc
      781 aattgccaaa acaacgaata cagctgaaat aaaaactttc tttgaatatt tcatagtacc
      841 tcctatttaa ctaatccaac actttttatt tccattttgt aatagacgtt tatatctgcc
      901 tttgcaaaaa cttcatcata tttatccatt attttttcat aatccctttt gtgatactta
      961 taaagtttat ccttaatctg cagtaaatcc gcattgtatg tatttttgag cttatccaca
     1021 gcatttgttg aagtttcgga aattttctta atggattcat ctttcaccct attcagaaaa
     1081 ttcctgtctt caaggttgtt gttgccgatt gtgaaagaat caatgtaaca gtaaagagta
     1141 atataataat ctacttgaag cttttcacct tgtaatttta tattgtctgt catacttaca
     1201 tcaacagcac ccaaagatat gtttgtgtca tctatttcaa tatttatatt cctgatattg
     1261 gcatttgccg ggtttaatag aagcattact gcttccaatt cgtccggttc caaaaaaccg
     1321 attaactcat attccttaac tatggctgct ctttctattt tcaaactttt ttcatttatt
     1381 tctaccacag gtatcattgc attacctttt tgcccaagat ggagaatagc ctcatcaaaa
     1441 gtaaatatat cttgataact ttctcttttt aatttaacaa gcatatcgct cagatatctt
     1501 ccaacaatgg gattgctggg tatttcagta ttgattatgt cttcagccct cccctttgct
     1561 atacaaattt ttactctgcg gttcatttgg ggagagcgtt ggatttcatc aaaaatttct
     1621 tttaatgcat ctgaatctct tgccagctct tcccccagca ctattacctg cataagtctg
     1681 tcggaaatta ctttatccga atgaaggtag tttagatggt aaaaatctga aaagcttttg
     1741 ccttcatttg tataaaccag cctatcttct tcgcttcctt catttatttt tggtatttcc
     1801 gaaataaatt ttaaactatc atcctcttta tcaataccga ctgcaaaaag atattctctc
     1861 ttattgattt caacactgtc ccagcatccg aacagaaaaa cagttgatat tatcagtaaa
     1921 ctcagtatta gttttttcat atagacctct tatataaacg atatactgtt ataaaatttt
     1981 ctcgttgtca tgagattctt cactgcatta ataatgacac gattattcct aactatgtct
     2041 tttttctatt acctgcatat gccaccagca tcggtataaa aagattcata ccgcttatta
     2101 tgtatatggg catttgaata tacggaaaca tcatgtacat tcccggcagt cctgcagcca
     2161 ttaagtataa aataggtatc tgaatgaata tgaaataacc attttcattt gttttaaatg
     2221 tttcctgcat agcaagattt gtaaaaaatg caagtatgca tatgcttgta aaaataacaa
     2281 ttatttgtag gcataaaccc acaatttccg tactttcaaa gaagaagccc ggaatattta
     2341 caaatttcat aatagaaaca aaaggccaaa caaggttgga agcttccttt gttccaaact
     2401 ttacaataat caatatatag ctaaaaatgt acaagaaggc gcttataaca agatacctct
     2461 tatttattct catgtttttt tccttggtat ctaccctgct gttagaaaac aaaagtacag
     2521 agaatccgag aaaactcaac atggcatttg gtactgattt aataattcct tcagcatccg
     2581 caggatatac cgggaaaata tttgtatagt ctgcataata tgttgaaaaa actattatca
     2641 gcaaatatgg tataagcgat gttaaaagag caatatttgt tatattggca atagtttgta
     2701 tacctttttt cgttgcataa gaagcagtca ataaaattgt tataataata gtgcttatag
     2761 gagttctaaa caacatcatc aatttaatct gctcggcaaa atcctttatt atcagactat
     2821 tgaccgttat aaacatcgat atgtaataga gtccgataat tttagaaata aatttagaaa
     2881 aaagtttatc cgaaacactt ataatcgtat cttcatcatg ttttttcatt gctttattaa
     2941 ttgtctttat tgaaaaataa agtataaacg ccgcaattaa tatcgaaatc catccttcgg
     3001 gaccgtcaac tcccgtaagt attacaggca ttaacataga ctgcaataca aatgatacca
     3061 atagtaaaat cgataaattc tgataaggtg taatatcttg attaaacatt tttcctctct
     3121 tttttctcca tatgcagata atccggtttt ctgaccattt tgctaattct tggtcttaca
     3181 atcgtatcct ttaagtccct cttattttca acaaatcttg taaatggtgt catgtaaggt
     3241 attccgcagc ttttcaatgt gcaaagatgg attatgagaa agcttaagcc tatggatatt
     3301 ccaaacaacc cgaatgttgc tgccaatatt atgaatccga atcttataag ccttaatgtt
     3361 gcaccaaaat tatagcttgg tattgtaaag gaggctattg cagtcaatgc aacaacaatt
     3421 acagccaaag gtgtaatcaa gcctgcttct accgaagatt gaccaataac caagcctccc
     3481 accagaccta tggttgagcc gacaggagtt gtaattctaa aggatgcctc ccttaccagt
     3541 tcaattacaa gctccatcaa tagggcttca aagtaaggag gaaacggaac atttgctctt
     3601 gaagctgcta catacagagc aagctgagta ggaaggaggt tgggatggta ttgggtaaca
     3661 gccacataca aagcaggcaa cagcataaca atgggcaggg caaaatacct taatattcga
     3721 gttgcacagg ccggcagcca cctttcataa tagtcttccg aagactgcat gaatgaaaca
     3781 aaaattgcgg gagcaatcaa gacggaagga gtattatcta ttgctattac aactcttcct
     3841 tccaatagtg cagatgcagc agcatcaggt ctttcagtat tttcaatttg aggaaaaggc
     3901 gaatagccgt catcttctat catttcttca atgtatgaac tttcaagaac ggcatctatt
     3961 tcaatattgt tgattctttt ttcaactgtt tccaatactg tattgtttac caaatcttct
     4021 atgtacaata tagctatatc tgtttttgag cgatttccta cctgagtata tttaaccttt
     4081 aacttagggt ctcttattcg tcttctgatt aatgaaatgt tcaccttcat tgtttcatta
     4141 aatccgtctc ttgcacccct tatgagggtt tccgcggaag gctcctgtac acctctcatc
     4201 ggccagcctc tcgacgaaag cattatcccc ttgttgcagt tttccaataa tagtacggtt
     4261 tcaccggaaa gaattttatc aacacattcc tcaatggttg tcagtatttg aatttccgaa
     4321 attgaaatgc ttgttctgac aattgcatcc tgcaattgaa catttgacat attttgaatt
     4381 ttttcaaggt caagaccctc aagaagagag cttattgcat actcgcttat tgcttccttg
     4441 gaagtcattc catccacata aaacaaaagc attttaagct ttttaatctt gcccccttct
     4501 aattttctga atattatgtc atcggtattc tcaaaaatct ttcttaaaat ttgctcattt
     4561 ttatttaaat ccaaatctat tttttgagtt aaatcaatat ccttcataat ctcccctacc
     4621 agtatattgc tgtggcagct gcaatagcta aaagtccgta tattattccc gtaaaacgca
     4681 ctattttata gtctctgtgg taattgtttc tttttaattc cttgcttgtt acaaatatta
     4741 atatatagct tataattaaa aagactatta ttacatcgtt gtttattatt ctttttaaat
     4801 cattaataaa aaacatggac cctccttaat gggataagct gttagtaata tctgcatttt
     4861 ttaagttttt ataccataaa atgaaaaaac attataaata caacaaaaag actcctttat
     4921 gttaataaaa gagccttatg acagtacccc cgtccccttg tcatctatag gttgtatttt
     4981 tgcttttttc tgaccaatgt tgaaatatct atacctaatc tcatcgatgc ttctttcata
     5041 ttctttgaaa cagctaaggt ggattcaata atttcttttt catattcatc cattttttct
     5101 ttcagtgttt ttgagcctgt tataaatagc tcagaaagct cagtcagctc tatgctatca
     5161 ggcaaaatca aattatctct gaacaactcc gttccgattt ctgctccctc tgccattaat
     5221 accaagcgct caattatgtt tttcaattcc ctgatgttgc ccggccactc gtatattaaa
     5281 aaactttgaa gacagcttga agtaatatag cagtttttac cgtatttatt gttatagtac
     5341 tcaagaaatt gagtagataa aggaataatg tcttccctcc tgttccgtaa gggaggaatt
     5401 accaatgtca caacatttaa acgatagtat aaatctgctc tgaatttttt ctcttccaca
     5461 agctgtttca aatcatgatt tgttgcagca ataattcgcg cgttaaattc tataggactg
     5521 gtgcctccta tgcgataaaa cattttatca tttattgcac ttaaaagctt aacctgcaaa
     5581 gtatatggca tgtcccctat ttcatccaaa tacagtattc cgccgttagc ttcttcaaaa
     5641 agtcctttct tccctccttt taaggcaccc gtaaaggcac cttcttcata accgaaaaac
     5701 tctgattcga gcaaatgctc aggtacggca ccgcagttaa ttttaatgaa cttacccttg
     5761 cttctgttgc tgatgctgtg tatatatgtg gctataaagt cctttcctac acctgtttcg
     5821 cctaaaatta aaaccgtact gtcaaccttt gatatttttt ttgctttttc gtatatattc
     5881 ttcattttta cgttatttat aatttcatca gggtcacgtg ctgcttcggt tttaacattg
     5941 taatcacttt taataaataa gttcttttcc tttgctatag ttttcatcaa catatccaat
     6001 tcggtcacat ctcggataac agcaattgtc tttgacagat tgccgttttc atcaaaaata
     6061 ggcgagcctg aaaccaagca gcgctgccct ttatagtaat ggtttatggt tgatacgggt
     6121 tctaaggttt ctataacctt tgcgcagcat gaatttggca gtatgccgct ttttctcaaa
     6181 tcgtaaacgg tattgcctat aatttgttct cttgtgagcc ctgataaatt taagaatgca
     6241 tcattagcat atatggttgt tccgcgttca tcagttatat aaatgccatc ttgtaatttg
     6301 ttgagcattt cgttatatat gtattgctga gagttgagcc gttcaatttg tttttgaagc
     6361 tcgcttccta tagtaaaatc ataaaagaaa aaactatata attcatggtt gtatatttta
     6421 aatttgttat ggaaaacata gtatacggaa ttatctttgc atttaacgga ataaagattt
     6481 ttatccatat caacgttaat atcctgaaaa aacattgtta tttttttgtt tagtatatcg
     6541 gtttttatta tatttgtatt aaaatattca ttgcaatata atacattgaa tttatcgtca
     6601 gttataataa aacccaaatc taaactattt ttaatcgatg acataatcct cttccttata
     6661 ttgttattac tttattatat atatcctgaa aagcacattc ttcattttgt acttagtata
     6721 tcgtaccact attattatat atgtacttat gcaattatgc aataatttta ttgtgtctat
     6781 atgcattctt gcatattttt gaaatataaa ttgagcagta atgttaaagc tccaacagaa
     6841 attacataat aatgttggca taaaaaatgc aacatattac ggcagacaaa ctaattatca
     6901 tagaattgct attataaaaa aatttaggag gaaattatga aaaaagcagt aattgtttca
     6961 gcggcaagaa cacctatcgg gtctttcggc ggtgcttttg cttcactttc agcagttgat
     7021 ttaggagtaa ttgcggcaaa agaagctatc aaaagagccg gtatagagcc gtcaatggtt
     7081 gaagaagttt atttaggcaa tatattaggg gcaggattag ggcaaaacgt agcaagacaa
     7141 gtgagcttag gagcaggtat tcctattgaa acacccgcat tgacaattaa tatggtatgc
     7201 ggctcgggct taagagcagt aagtatggcg gcacagtttg ttgaaagcgg ccaatgcgac
     7261 ataattttgt gcggcggtac ggaaagcatg actgatactc cgtatctttt gccaaaagcc
     7321 agatggggac atagaatggg tgacggcaag gtggtagatt acatggtaca tgatggacta
     7381 tgggatattt acaatgatta tcacatggga atgacagctg aaaatgttgc agagcagtat
     7441 ggcattacaa gagaagaaca ggatgaattt gctgcacaaa gtcaaaacag agcagaggaa
     7501 gctcaaaaat caggaaaatt tgcagatgaa atagtacctg ttattatacc tcaaaaaaaa
     7561 ggcgaccctg tagtaattga tacggacgaa tatccaaggc atggttcaac cgttgaaaaa
     7621 atgtcaaaat tgaaacctgc attcaaaaag gacggaaccg ttacagcagg aaatgcatcg
     7681 ggcataaacg acggtgctgc agctttaata gtaatgagca aggacaaggc aggtgagctt
     7741 ggattaaaat atttgtgtga aatagcaggt tatggctcag caggagttga cccgaaggtt
     7801 atgggcttag gacctgttcc tgcaaccaaa aaagcattgg caaatgcagg atggacaatt
     7861 gatgacctgg atcttattga agcaaatgaa gcttttgctg cacaagcact tgcagtaatt
     7921 agagatttag gattaaatcc cgagataaca aatgttaacg gcggagctat agcattaggg
     7981 cacccgatag gagcatcagg agcaagaata cttgttactc ttttatatga aatggaaaaa
     8041 agagatgcca aaagaggtct tgctaccttg tgcattggcg gcggaatggg cacatcttta
     8101 ttggttcaaa gataacaatt cggtttgtca ttcatagcaa tgacaagttg ttgtactata
     8161 aaaagaaagg atgatattat tgataaataa aataatttct atagatgaag ctgcaaatat
     8221 ggttaaggaa ggcatgacaa ttatggttgg gggatttttg gcaaacggaa ctcctgaaac
     8281 cttaatagat gcattggtag aaaaaaatgt taaaaacctt acccttattt gcaacgatac
     8341 aggttttccc gataaaggag tgggaaaaat ggttgttaat aaacagttta aaaaaatgat
     8401 tgtgtcacat gtggggacta atcccgaaac agcgaatcaa atgaattcgg gagaaacgga
     8461 agttgtactt actccccaag gaacacttgc tgaaaaagtt cgatgcggcg gaaacggtct
     8521 tggtggtttt tacacaccaa caggtatcgg gactgaagtt gcagaaggaa aagaaataag
     8581 agtaataaac ggcaaggagt atatgtttga aactcctttg agagcagatg tggcattatt
     8641 aagagcttcc gtagctgata aaaaaggtaa tatgatattc aacaagacta tgaaaaattt
     8701 taacccgata atggctacag cagcagactt agttatcgtt gaagccgata aaatagtgga
     8761 agttggggaa atcgatccgg agcatgttat gtgttcgggt atatttgtag actatatagt
     8821 tggaggtgac aaataatgga tgcaaagaac gtaatagcaa aaagagttgc tgaattgtta
     8881 cacgacggtg atgttgttaa cttaggcatc ggtcttccta ctatggttgc aaattacata
     8941 ccggaaggaa tggacataac atttcattca gaaaacggat ttttgggatt aggtccgtgt
     9001 cctaaggaag gagaggaaga ttgggagctt gtaaatgcag ggggaatgcc ttcttcaata
     9061 gttcccggcg gaatgttctt tgacagcgcc acatccttta gcattatcag aggcggacac
     9121 gttgatgcaa cagtcttggg agcaatggaa gttgatgaaa aaggaaatct tgcaaactgg
     9181 aaaataccgg gtaaaatggt tcctggcatg ggcggagcaa tggacctggt tgtaggtgca
     9241 aaaacagtta ttattgcaat ggttcatact caaaagggga aaccgaaaat attgaaacaa
     9301 tgtactcttc ctttgactgc taaggagcag gttgatatca tagttaccga aatggctctt
     9361 atcagagtga caaaagacgg cttggttctt gaagaattag gacccgaagc aacaattgaa
     9421 gatgttgttg ctgcaacaga agcaaattta atcatttcac cgaatttgaa acgttttggc
     9481 gtggaagaag aataatataa taaaaattgt cattctgaag gcgcagcctg aagattctca
     9541 cttttcaata atgatgagag ccttcgcttg cgctcagaat gacataaagg gatttgttat
     9601 taatatgaaa caggagctga tctcctgttt ttttaaatgc cgtcgggtct gtttcttaat
     9661 gttaaaacga gttttagttc ttcaccgttt cttaaaattt taaattccga tttatctcca
     9721 tcttgatatt cgtatagttt tttttgtata tcaaccatat attctgtctt tttcccgtca
     9781 atttcaataa taacatcatt tggctgaagt ccggcttccc tggcaggaga atctttcatt
     9841 gtttctatta taattactcc atagtctgcc attaaatcga ctccgtaaag catttcataa
     9901 gtttcaatgt ccatgcctgt gatgcccaca tatacgggta taaaagttcc tcctgtaacc
     9961 acttgtgtca aaaccggttt aattatatta atcggtatag caaaacccaa tccttctgct
    10021 gaggtcattt tggccgtatt gattcctatt acctgtcctt tggcgttaaa caaggggccg
    10081 ccgctgttgc ctgaatttat ggaggcatct gtctgaataa gcggctctat gacagtgccg
    10141 tcttcaaatt ggatagacct gttaaggccg cttataatac cttgagtaac tgttctttcc
    10201 aggtctaagg tcattggatt accgattgca accgcaggct ccccaaccat taattcgtcc
    10261 gaatttccta attcggctgc gggaagtctt gttttattta tctttacgat tgctaaatcc
    10321 aagttggtgt cataccacaa tacattaccg gtttctattg agccgtcaat taaagaaact
    10381 gtaatatttt tataattacc atcacctata acatgagaat ttgttacaat gtatccgttc
    10441 gaatctacaa taaacccgga gcccatatgc tgctctgtcc ttttctgggg accccaaaat
    10501 gtattaattt gtctttccac ctcggttgtt attcccacaa ctgttttttg tgatttttct
    10561 gcaactgcta ccgcaaaata aacatcatcg gttaaattaa ttttaacctc ttgagcatta
    10621 attgatgact cagctgcttt tttttcagct gccgcgttta acatatttgc tgttacataa
    10681 ctgcccgtta gacctcctat tattgcagct actaatacaa gggcaatgta acccataaca
    10741 gactttattc ttcgcttttt gggtttctta attaaaggct cgttcatgac aaaattaatt
    10801 tcaccgtatt catttgacat actatcatta gttatattat tttgattttc tttttctaaa
    10861 ttatcgttaa aattattttc gaagtccatt aaacaccgct ccttatttat tttaattttc
    10921 gtttattcaa tatagtataa ttatatcctt cttttcttta ttgaaactta aattaataaa
    10981 taaaataaat cgaaagaatt actgttactc ccgcgacaga ttatcatcac acaggtcaat
    11041 tcttaaaaat caggcaatta aaaagatatt gaattatgtc agtttttata tataatgtta
    11101 tatacggagg aaaaatgaaa aaattattta aaatagtcat tcaaatagca ataatttatg
    11161 caatatacca ggcaggaaac tttataagca gcctcatatc taatataata gttatccccg
    11221 gcaatattat aggcatggtg atacttttgg tattattgat aaccaacgtt ttaaagtttt
    11281 caataattga agaaacatca aattttatgc ttaaatacat gagttttttc tttgttccca
    11341 taacggttgg aattatggaa tcgtacaaac tgatacaaga tagtattgtc caaataatta
    11401 taattttatt tatatcttgc atattagtta tgtacgtatc ctgcaaggtt acggatgtac
    11461 tgatttcata cagggagaaa agccatgatt gacatattaa cagctctttt ttttatattg
    11521 cttacaatta ttgtttttaa atttagcctt cttgttaacg ataagttcaa aatagtattt
    11581 ttgaatccga ttcttttaac tgcctttatt ataatagtta ttttaaaaat atttaatttt
    11641 acatattctg attattacaa aggtggaaaa atattagtat ctgcattagg acctattgtt
    11701 gtagtgcttg caatacccct ttataaaaac agacatgact tagtgaaaaa ttttattcct
    11761 attgtaggag gaataatatc cggcataatc actacatttg tatcgataac agtcttatgt
    11821 aaattgtttg gaattgatga aataattttt ttatcactct tgtccaaatc agttacaacc
    11881 cccatggctg ttgaatcaac taagatattg ggagggaatg aagctgtaac tattctagca
    11941 gttataatta ccgggttaat aggtgctgct tttgcaccta tagtaataaa caaaggcaaa
    12001 ataaagaata atattgcaaa aggaatagga atcggctcgg cagcccatgc attgggtact
    12061 tcaaaagcaa ttgaaatgaa tgaggaagca ggcgctgcaa gcggtcttgc aataggctta
    12121 accgggttat tgaccataat agccgtaata atttttgcat aggagacaaa tatgattaat
    12181 ttatctgaaa ttaaaaagga agatatggat agtatataca agtggtttgc cgacactgag
    12241 tttttaaaat attatgatta ttacccgcct aatcctttag ataagtctga tgtagataaa
    12301 atgtttaaat attatgaaga aagcggaaaa tcgaaagtgt ttgccgttag aaaagaaagc
    12361 actattattg gagttgccgg ttttgatgat ataataaaag aaaatcaagt tgcaactttg
    12421 tttatcggtt taggcagcga aaatgaaaga ggcaagggat acggcacaga aactatgagg
    12481 cttttgttga agtatggttt cgaaaactta aattttcaca gaattcagct taatgtattg
    12541 gaatttaatg ataaagcaat tgccttatat gaaaaatgcg gtttcaaaaa agaaggtatt
    12601 ttcagagaat ttgtattaag agacggtaaa agatataatt tgcttttgta tggcttgtta
    12661 aaaagtgaat gggaaaaaac tgagatcctt cgcttacgct cgtgatgaca atcacagacc
    12721 atattccttt atcgcggtaa gataaactcg tgacaattat aagggataga ctaaagaatc
    12781 tcagattttc agggtatgag gacacacctc cactcttagg atagtttccg aagtaagagg
    12841 aatgtcctca tacatgagaa tcttcgctat gctaaggatg atattatgca atattgctgt
    12901 caatcattct tgcaaaagaa gctaagggtt ttactccgat ttcttttgag gcaaactttc
    12961 cttccttaaa aaatattata gtcggtatag tttgtattcc gtagcgaata gctaattcct
    13021 gctgctcatc aatatctacc tttgcaacat taattttccc gctatattgt tcagctactt
    13081 tttctattac cggtgcaacc attctgcaag gagtacacca ttctgcccag aattccgcca
    13141 atacatatct gttttgttta atcatgtcgt cgaattcctg agtactgttg ataattttaa
    13201 tattttctgc catttaaatt ctccttttta tctatttgta gtgctgcatg ctgagcagct
    13261 atcaaacctt cacctattgc ttttgaaagc tgaaggggtt ttcctgtaat gtctcctgcc
    13321 gcatataatc ccggaatatt ggtttccatg tgtttattgg tatttataaa attaccgtca
    13381 agttccaaac catcaatcaa tgcagcaggt gcaaccgcat ctcttagcat aaatacggca
    13441 tcggttttta taagatcatt tcccgtatta acagcttcaa cagtgttttc accaattatt
    13501 tcggtaatgg ttccttcaat gaattttata tcattgctta aattttcttg ttttggtttt
    13561 tttgatataa aggttacatt cactccgata ctttgcaaaa aatttgcttc ttcccatgca
    13621 tcttttgatt ggtcccatac aagagcattt ttacctctgt aaagcattcc gtcacatgta
    13681 gcgcagtaac tcactccgtt gcctaagagt cttccttctc ccggcaattc tttgactttt
    13741 gttattccca ttgccaatat tacttttttg gattcaaaaa tttcaccgtt tacattgacc
    13801 ataaaacttt cacctaatgg aagtatgttt acggccatac cttcctctat ttttatatcc
    13861 atagcctttg catgttcttt aaacttatcc atcaattctt ttccgctggc atgataaaaa
    13921 cctaaatgat tatcaacacg ctcggctttt gacagattat tttctttatt tgaaaaaatt
    13981 cgtacagtct tgcccctggt aacagcattt actgctgcgg ataaaccggc aggaccggct
    14041 cctataatga taatatcaac tatatctccc atattagcct cctttcttca atgtattaca
    14101 gtctcacgca gtctcattca ccaatttatt tgctcctttg tcgcataaat tgggaattcg
    14161 ttgcgaatga cctaccgctg tttttttctt cgctttggct cgaaaaaaca gggttaggac
    14221 atctaaaagc ttaggtttct gcttattgca gtgaatattc ccgtaaatgt cataattcct
    14281 attattatta aaataatacc gccaatcttt attgaatatt tgaccagttg tcttttttct
    14341 ttaaagaaat tcaatgcttc ggtggtaaat actcccaata ttataaaggg tattataaat
    14401 cctaaggcat aaacaaatac caacatgttt ccggcaaatg cattggtggc atttgatgcc
    14461 agaattaaga ctgaagccag tgcaggacct acgcagggag tccatgcaaa actgaatgta
    14521 aatcccatta tgtatgcaag tattggattt attttcccac cgatatttag atttatcctt
    14581 ttttcaactt tcaaaaaatt taactttatt atgttaagtt gaacaaaacc cattattact
    14641 attattatgc cgccaatttt actaaataac tctctgttat tattaaagaa tatacccaaa
    14701 gttgaaaatg ataaacccaa aataaaaaat acgctggaaa tacctaatat aaaaaataaa
    14761 gtgttaaata gtaccttttt tctttcaaat gtaatagtac catcgctgct tatttttctt
    14821 ccattgcctg ccaagtagct gatataaaca ggaattaaag gaagaacgca tggtgaaaaa
    14881 aaagataaaa acccttcggc aaaaaccaat attatactta cattagttga taaacctgca
    14941 cctcccattt tgacctcccg aaataatatt attaaaatat agcagggcaa ctttactatg
    15001 tcaagtaacc ttgtcacata tgtttcaaca gccacggacc ttgcccgtgg ctgttattta
    15061 tcttattgat attacttcta tccatttaac caaccgagtc agtaatgaag tatcatatca
    15121 ttctgagggc atgcccgaag attcttcctg tacattctca tctcgagctt cttgttcttg
    15181 ctggccgttt attaaagcat taagctggtt taagagcctt tccaattcat taatttttct
    15241 gccgtattct gcccaatttc cgttttgtga agcttcattt gcatcattaa acacttcatt
    15301 ggctttaata attaaatcat taatatttgt atcatcatat tcttcgtcag gcacctgtgg
    15361 tcttccgggt tcttgctcag ttatgccgaa aagtctgtta atggctgtat ccagtgtagg
    15421 ctccatcaat attttatcgc cataggatac aacaaccatt ttcatttcgg gaaaattgct
    15481 gtctgtatct gatttcaaat atatgggctc tacatacagc aatgattctt ctattggaat
    15541 taccaacgtg tttcctctca aaacatctga acccaattga ctccacaagg taagctgtga
    15601 tgatatattc gtatcttggt caatttttgt ttctatcata ttaggtccgg gaattacttt
    15661 tgtttttgga aattcgtaca gcacaagttc accgtaattt tcgccgtcat ttcgtgcagc
    15721 caacaatgct atcatattat ctttgttctg gggagtatat tgattggtca ataaaaactc
    15781 cacatcttcc tctcctggaa gtttaaacat cagataactt gaacctgcag gtacttctcc
    15841 tccacccata tatttctcgt ttgctaaatc ccaataatcc tctcgcccaa agaaaaccgt
    15901 tacattttca atgtggtaca atctgtacat atctgattgt acatcgaaaa atgcacgtga
    15961 atatctaata tgctttctaa taccttccgg catttcatct atcggtttca ataaatccgt
    16021 aaaaattttg tcataggttt ttataattgg gtcattttca tctgccacat aaaaatcaat
    16081 tgtgccgtca taagcatcta ttacaacctt tacggagttt cttatataat tgacactcat
    16141 acccttaata aatatatctg taggttgtga ataagggaat ctgttgctta cggtaaatcc
    16201 ttcaataatc caatacaatt taccgtcatc ttgattcact acaatatatg catccgggtc
    16261 atagtacata aaaggagcta tttcagttac cctttgcatt atatttctat ttattaaaat
    16321 tttggaattg ctgtcaatat tgttggaaat catcattctg tagctgcctt ttcttatgct
    16381 gaacataagt ctgttgaaaa aagaaaggtt gattcctgct ttaccttcgt atatggtttc
    16441 aacattatcg gcacctgaag gataatcaaa ttccatttcg tctgtgttta caattacata
    16501 attattggtt ttttcgccaa aatatatttc aggtctctgg atattaaagt ctgtttccgt
    16561 tgtgggagga atatttttaa ccagtatttc aggctgcccc tgtggtgtaa cattattcac
    16621 ggtagatacc gttattccaa atccgtgagt atattttaaa tattgattga cccaggttct
    16681 tgcctgttca tttaatctgt cttggtcaag ttctctcgct gacaaaaaaa cctgcttgta
    16741 atcgccgtca attacatatc tgtccacatc tacatcatta aagacatagt agggtctgat
    16801 accttgcagc tggttataaa cctgaattaa cggctcctgg tcatttattc tgatattgtc
    16861 tattacttca gggttgtttt gtaaatcttc aatggttaaa ttattattgg caggaaactg
    16921 tattgttttt acattgttta aagaataagc attttgggtg cttttgatgc tgtattgcat
    16981 atattttgtt tcttttgaca attggtcagg ctcaactaca aatttttcta cggcacctgc
    17041 cacgccggtt ccagcaattg atatgataat aagagctgcg ggaactgcca atgccgtttt
    17101 taaatttctt tttcttgcgc cgataaaaaa tgttatagct gcaagtacac atccaacggc
    17161 taatattctg tatagattca aagtaacgtt aatatcagta tatcctgcgc cgaatacacg
    17221 gcctaaacgc gaaaaaagca aatcatagct tcttaataca tatctcacag ctaacagtaa
    17281 aaataaaaaa acacctatta ttgaaagttg atttattatt ctttcaacaa actttttatt
    17341 caaaatatta ttcaagtcta tctgcttacg tggaaattgc tgtatatctt caaatttttc
    17401 cgaaacattt tttatgctgt ccttaagtgc aagaaagccg ttaaacaata ttataattac
    17461 tgtcagcaaa aataatatgg aaataatcga acttacaatt gtatttataa gaggtagttt
    17521 aaatacgtaa aaactcacat cattattaaa gatagggtcg gttgctccaa attccacacc
    17581 atttatatat tgaagaattt caaaccacat ggtggatatt atattcaatg ttataagaag
    17641 acttgctcct gcagaaatta atttaatagt taaattaaat tttttattta caacgattat
    17701 ttctgtttca tcatcatatt tcttctttag tttcttaata aatatgtaca ataagatgct
    17761 caatataata aaaatcggaa taccaatcat aatttgcgct ttaattttgg taaaaaatgt
    17821 ttgggtatag ccgacttctt taaaccacaa ataatttgta ataaattcta ctatattggt
    17881 aaaggaattg ataatcaaaa aaacgatgac aacaattccc agtacaaata atcttccttt
    17941 attcctcata ttacctcctt ttatctgtag tattcaattc ttaatttttc caaagatttc
    18001 ttttctattc tggaaacagt catttgagat atttttaatt ctttggcaat atcactttga
    18061 gttttatttt cataaaacct ctttataatg atattttttt caagttcatt caatttactc
    18121 atcgatttgt tcaaaaaatc caaattttct attagattgt aatgtttatc ctcagctccc
    18181 acaacatcca taagcgatat gtcattatca tccctgctta aatcaaattt ttcatcaagg
    18241 gattgagaat taaacattct tcctgcctca aatgcttcca atacttcttc ctctgtactg
    18301 cttatatatt cggcaatttc ttttattgtt ggtgccctgt gaagccgggc tgtcaaaata
    18361 ttgttgtatt cattgacttt tcttgatatt tcctgaattt ttctgggcac cttaacagcc
    18421 caacccttat ctcgaaaata tttttttatt tctcccaata tggttggtgt tgcaaaactt
    18481 gtaaattcaa atccttttga tatatcaaat ctttctatgg caaaaataag ccccatgcat
    18541 gcaatttgat atatatcgtc atgctcgata cctctgttgc tgtattttct ggatataatt
    18601 tcagccatat atttatattt ttcaaaaatt tgatttctta tttctatatc tttggtttca
    18661 tgataaagta aaaacaaatc ataattttta atacgcttgg agtttttact cggttcaatc
    18721 atattaatca acgcctattt tatttttttc aaaagttatt gttttattat ttaaatcata
    18781 attgaaatta tcggacaaac tttcaataat caatatgctc atatcattat ttaaactttc
    18841 ttcggataat tcaccatcat ttaaatccgc taccgtcact ataatcttat ttttgaaaac
    18901 ttgataagat atttcaaaat cttccgtgct gcggcttaaa ttcattataa aaaatgtgca
    18961 aatttcagat attattacct ttatatcttc aatttcatca atattgaatc cgtttagatt
    19021 aaatagtgac gacgatgtta atcttattgt gctcatatat tcggattttt ttggaatttt
    19081 caacaatatt ttatccattt catcactccc ggatattaaa cactttgttt aaacctgtta
    19141 tatcaaaaag tttataaata tttggcttta aattgattat ttccagattt aaactgtttt
    19201 cctgcatttt tttataaatg ctcatcaaaa caccaagccc tgtactatcg atatactcta
    19261 agctttcacc gtcaatgatg atgctggtat ttctttcttc aatcagtgaa aatatttgat
    19321 ttttcagctc aggagaagtg tatatgtcta cttctcctat cggttttatt tctataatgc
    19381 tatcctgaat tttgctttca atttccaatg acatattttt tacctccgtt attcttcctt
    19441 tttcaatctt cctctgaaaa aatctttgct attgaggcaa ctttgtcgtc acccttcaat
    19501 ctcattgcaa taacaccttg agtatctctt cccattatgg aaatatctct tgcatgcatt
    19561 ctgattatcg ttccgcttgt acttaaaatc ataatgtcat cttctttatc aactattagt
    19621 gaattgacta taagccctgt ttttttattt atgttgtggg ctttcatacc ttttccgccc
    19681 ctgctctgag gtctgtattc ctgagacagg gttatttttg cataaccgtt ttctgttacg
    19741 gttaaaatgt gtttattttc atcgcttttc ctaataatgc tcatggcaac aagctcatcg
    19801 tttttagaaa gtgttattgc tttaactccc atggtgtttc tgccggtttc tcttacgtcg
    19861 ctctcatgga atcttatacc ctttccttgt ttcgtaacaa tgaatatatc ctttgttccg
    19921 tctgttttgt taacacttat caattcatcg tcttctgcaa tttttatagc caccaaacct
    19981 gtctttctgt ttgattcaaa ttcgctgagc ttagtttttt tgataatacc cttcttggtt
    20041 acaaaaatca aaaattcatt atcgttaaat tctcttatcg gaatcaatga ttttattttt
    20101 tcgcccggtt caatgttaat caagtttatt gcagctgttc ctttagcctg acgtcttgct
    20161 tccggtactt catatgcctt tattttatat attttccctt tgtttgtaac aaacattaaa
    20221 taatcatgag ttgatgttat aaacaaatcc tttacaaagt cttcatctct tgtggttagt
    20281 gcggtaattc ccttaccgcc tctgttttga accttataag tatcctccgg aagcctttta
    20341 atatatccga aatttgtaag tgttattgca acattttcct cctctattaa atcttctaca
    20401 ttgatttctc cttcatctgc tgtaattttt gtttttcttt catcataaaa actattcttt
    20461 atttctttta attcatcctt tattatttgg tcaatcaaat tctgattaga tagaatttct
    20521 ttaaacttgt ttatcatttt tatcaggtct tcatattcag catttaattt ttctctttca
    20581 agaccttgca gacgtctaag tctcatatct actatggctc ttgcctgtat ttccgttaaa
    20641 ttaaatgctt ccattaatct tttctcggca tcgtcatagg atgctctgat aattttaata
    20701 acttcatcta tattatcaat tgcaatcaat aaaccttcaa caatatgtgc tcgggcctca
    20761 gctttatcta aatcatactt tgtccttctg acaataactt ccctttgatg ttctacatag
    20821 tggtatatca tttcttttaa gtttaaaacc tttggctcat tatttaccaa agcaatcatt
    20881 attacgctga agctgtcctg catctgagta tgtttgaata attgattaag tactatgttg
    20941 ggattataat ctcttttaat ttcaataaca atcctcattc cgtctctgtc gctttcatcc
    21001 ctaatgtcgg aaattccttc aattattcta tccttcacaa gatcagctat tttttcaata
    21061 agtctggcct tattcacctg atatggaagt tctgatacta atatttggct tttattgttt
    21121 tttaattctt ctatttcggc tttagcctta acctttaata ttcctcttcc tgtcaagtaa
    21181 gcatttttaa tgttgtcttt tccttcaatt atggcgcctg tgggaaaatc aggacctttt
    21241 atatatctca taagtccggg aatatctata tcaggatttt caatataagc aattatcccg
    21301 tttattacct ctccgatatt atgaggaggt atagaacttg ccattcctac cgctatacct
    21361 gatgaaccgt ttacaagcaa gttaggaaaa cggcttggaa gcacaagggg ctccttcaaa
    21421 gtttcgtcaa aattcggtct gaaatcaacc gtatctttat tgaaatcacg aagcatctcc
    21481 attgctattt ttgacatacg tgcttcggta taacgcattg ctgctgcctc atcaccgtct
    21541 atggagccaa aatttccgtg tccgtcaatt aatggatagc gcatggagaa actttgtgct
    21601 aaccttacca ttgcatcata aacggcagta tctccgtgag gatgatactt acccagaaca
    21661 tctcctacaa ttcgggcact ttttttgtga ggtttatccg gtgaaagccc caattcactt
    21721 gccgaatata gtattctcct gtgaaccggt ttaagcccgt cccttacatc aggcaatgct
    21781 cttgatacta ttacggtcat ggcatagtct aaataagacc ttttcatttc ttcagaaata
    21841 tttatattta caattttgct tttattcaca ttttcgctca tttacaacct ctcagcttat
    21901 atatctaaat tctgtacata ctttgcgttt tcttcgataa attctcttct gggagcaacc
    21961 ttatctccca ttaaaacatt gaatatttca tctgcttcaa ttgcatcatc ttcattaatt
    22021 cttaatagaa ttctgttttc aggattcatg gttgtttccc aaagctgctc ggggtccatt
    22081 tctccaagac ccttatacct ttgtatttca ttcttattgt ctcttccgat ttcatccaat
    22141 acctgattaa gctcatcatc gctgtataca tatttttcaa attttccttt tcttactcta
    22201 tacaaaggcg gctgggcagc ataaacatac cctcctgtta ttagctcttt catatatctg
    22261 tagaaaaatg taagcagcaa agttcttata tgtgctccgt ctacgtcggc atcggtcata
    22321 attattattt tatgatatct taattttgaa atatcaaaat cttctcctat tccacagccg
    22381 aatgccgtaa tcatattttt aatttcttcg gaatttaata ttctgtctat tcttgatttt
    22441 tcaacattta atattttacc tcttaaagga agtattgctt gagttcttct gtcccttcct
    22501 tcttttgcag agcctcctgc cgaattacct tcaacaatac aaatttcaca gagagcaggg
    22561 tccttctccg agcaatctgc caatttaccc ggtagtgcca tactatcaag agcgcccttt
    22621 cttcttacca agtctcttgc ttttcttgca gcttctcttg cccgtgcgga ttttaaagct
    22681 ttttctacta ttgctttagc tgttttgggg ttttcttcgc aatatatgct taaagcctct
    22741 cccgtaatac tgtcaactat tcctcttgtt tcgctgtttc ccagcttggt ctttgtctga
    22801 ccttcaaatt ggggatttgt tattttaatc gacaatattg cagtaatccc ctctcttata
    22861 tcttcaccgg taattcctac ttcattttct tttattaaat ttgctttttt tgcataatca
    22921 tttataacgc gtgtaagggc agttttaaaa cctactaaat gagttccccc ttcaattgta
    22981 ttaatattgt ttacaaatga aaatatattt tcattatagg aatcggtata ttgaattgca
    23041 atctctacat ttactttatc ctttgttccc tcggtataaa taacatcgtt gtgaagaggt
    23101 tccttcttgc tgtttaaaaa ttgtacaaac tccctgattc ctccttcata atggaactca
    23161 ttcttaattt tttgtctttt atcttcaagg attattttta ttcccttatt taaaaatgcc
    23221 aactctctca ttctatgctt aatagttgta taattaaata ctgttgtatc aaaaattgta
    23281 gggtcaggag caaatgtaat acttgttccg gttttatttg tattacctat aaattcaacc
    23341 tctgtttttg caatacctct ttcatacctt tgcctgtact ttttgccgtc ccaatttact
    23401 tcagcaacca aatattcaga tagagcattt acgcatgaaa caccaactcc atgaagtcct
    23461 cccgatactt tataagcatc attgtcaaat ttaccgcctg catgaagtac tgtcaaaaca
    23521 gtttccaagg ttgatttgcc tgtcttttta tttgtttcga tagggatgcc tctcccattg
    23581 tctacaacca caactgaatt atcttcatta attgttaatt taatggtatc gcaatacccg
    23641 gccattgctt catctatgct gttgtctaca acttcataaa ccagctggtg aagtcctctt
    23701 tctcccgtag agccgatata cattccgggt ctttttctta caggctccaa accttcaaga
    23761 acctgaattt gatctgcgct gtaatgattg ttatttttat ctgtcgccat ttatttctcc
    23821 tctaatttga aatgttacta atttttcctt cgtttatata aaacattttt ttatcgaaat
    23881 ttttagttaa aatatttagc tcgtgctcat cagtacttgt tattattgtt tgtatatctt
    23941 taatatgctc aataagatag ctctttcttt tattgtcaag ttcagataat acatcatcca
    24001 ataataaaat aggatattcg cctgtttctt cctttattat ttcgatttcg gcaagcttta
    24061 atgctaatat tacagagcgc tgctgccctt gagagccaaa gtatttacat tccttaccgt
    24121 tgatttttat aattaaatcg tccttatgag gaccgtaaag ggatattccc tttaaaattt
    24181 cattgttaaa atttttgttg attttatcat aaaatatttt ctttacttca tctctgtccg
    24241 taatacatcc aatgttgctt aaatataata tgtccaattt ttccttgttt cccgatatac
    24301 ttgaatatat atccgttgct gcttttttta atttatttat gtattcattt ctgtaaagcg
    24361 atatttccgt acccgtattc accaggtaat cgttccaaat actaataata tccttatttt
    24421 cacattttgt atagtagttt tttaacaata gatttctttc agtggtaact tttttatatt
    24481 tgttaagcaa atatttatat ttaggcttga tttgtgaaat atcaatatta atgaatctgc
    24541 gtctttcaac aggacttccc tttataattt tcatatcgtc gggcgtaaaa ataacattat
    24601 ttaaaatacc tatcatttca gatgtttttt caagctttac accatttata ataacttgtt
    24661 tctttttatt tttttccaat aatatttcta ttttgatctt gtaattattt ttttcaatat
    24721 aaatttcaac tgaacttttg ttttcaaaaa agttaatcaa ttcattttcg ctgttcaatc
    24781 tgaaagttct ccctatggaa gcaatataaa ttgcctccat aagattagtc ttaccttgtc
    24841 cgttgtctcc gataaaaata ttaagggaat tatttaattc tgcttcagct tctttaaaat
    24901 tcctgtaatt tttaattata agttttttta ctatcattct tcctcatcag tatccccata
    24961 acttgcttgg tatgtaccta tacctaataa cgctcaatta aatacttttc attattgaat
    25021 tcaattatat ctccttctct taactttttt cctctttgca gaacaacctc tccgttaacc
    25081 ttcactaatc cgtccaatat gacattttta gccatacctc ccccttcaac ggcatttgta
    25141 tattttaaca gctggtctaa tttaatatat tccgtattaa tgtttatttt ttccatacaa
    25201 taccctttct aattattctg aaggctagcc tgtagaatct catgaaatcc ctcgctacgc
    25261 tcaggatgac aaacaggaaa cctgttaact gattctcacc ggcagaacca tgtaaatata
    25321 attatcctta ttttcttcat tttcttcttt tataaaacat ggattatttt ttcctattaa
    25381 attcaatgaa atcatttcac tgtctattac tttcaaataa tctaaaatat attttgaatt
    25441 aaaacctatt tttatatctt ccccttcttt atcaattgat atattttctt caacattgcc
    25501 gtattcggag gcagaattta tctgcatatt gaaatcttta atatccataa taatcaaatt
    25561 atttttatca tctcttgcaa gaagcgaagc tctttcaata ctgtctctca tatcttcggt
    25621 atttactttt acaacagtta cataattgtc tttaataatt ccttcatagt ctatgaattt
    25681 tccttccaga aggtttgaaa taataattgt attttcaact ttaaaggaaa tatgactttc
    25741 agacaccgtt atttctgttt catctttatt ttcttcaata atcctcagta tttcaaacaa
    25801 tgtcttagaa ggtataacta ttgaaatatc tcccttataa tcgatttttt ccttttttac
    25861 agccatcctg tacccgtcaa gagctacaac ggtacacaaa ttatttttta tttcaataag
    25921 acaacctgta aataacggtt ttatgttttc tattgctgca gcaaaatatg tatgttttat
    25981 taaattttta agtacttcgg attttatctt aaaagtttct ccttcattat cgaatgtatt
    26041 gttaggatat tcgtctgctg aatttccaag tatattaatt tcagaattta agcattttat
    26101 ttccatatta ttgtttccca tggtttcaat aaacacatat gtatttgatg gaagttttct
    26161 gacaaaatct cctattaatc ttgactgtac tacaattgaa ccttcttctt caatttcaca
    26221 ggggaaatat gtttttattc ctaattctaa atctgttgct gttaaagtta gaatattatc
    26281 ctttgcttca ataagtattc ctgacaaaat aggaagaggg gttcttgaag atacagcctt
    26341 ttgtacaata ttgatagctt tattaagttc attttggtta atttttaatt tcataataga
    26401 ctccttagta ataatattta ttgttgataa aataattttg cttgccatag tagaaaaata
    26461 taataataat aagtaatatt agtaggtatt gttaatttgt tgaaaactat ataaaagttt
    26521 gtattttcaa gattataaaa aatttatact gttgattgtt ttattattta tccaagacat
    26581 ataaaatctt tatgttttca taaataatta caaacagttt atcaacattt taaagttaat
    26641 tattaagcat tgaaattaaa tcttctatat tttttttcac ttcttgactg ttttccatat
    26701 cttcttcaac tttattgaag gcgtggagaa ccgttgaatg gtctctgcct ccaaattctt
    26761 cacctatttt aggcagagat aaatcagtca gttttcttgc tatatacatg gcaatttgcc
    26821 ttggatatgc aatatttttg gttttctttt tggattctat ttcatcaatc gaaatattaa
    26881 aataattagc aacaacttct ttaatatcag atgccgatac ttttttattt tttgatatca
    26941 ggtgctttaa tgcttcctga gctaattcaa gagatacttc gtttttgttc gttaaggaag
    27001 cataagcata aatacgaatt agtgctcctt caagcttacg aatatttgat tgtatatgct
    27061 gggcgatata tgtaattact tcatcaggaa tattataatt ttctgcttca gccttttttc
    27121 ttaatattgc aattctcgtt tcataatccg gcggctgaat atctgcaatt aaaccccatt
    27181 caaaacgtga gcgcagcctg tcttccaaag tgggtatttc agaaggcgga ttatcgctcg
    27241 atacaataat ttgtttattt gcttcatgta aagcattaaa ggtgtggaag aattcttcct
    27301 gggtactttc ttttcctgca ataaactgaa tatcgtctac caataataca tcggcttttc
    27361 tgtaggtatt tctaaattct tcattagtgc cgtctttaat tgaattaata agatcatttg
    27421 taaacttctc gctggttaca tacagaacat aagtgtcggg attattatct aatatgtaat
    27481 gtccaattgc atgcattaaa tgagtttttc caagacctac gcccccatat ataaacaagg
    27541 gattgtatgc tgttgaaggt gcttccgcaa cagctaagga agcagcatga gcgaaacgat
    27601 tgctgtttcc tattataaag ttatcaaatc tgtattttgg atttaatatt cttccgttat
    27661 atattgcatc cgtatcgtct aaactatttg tgttatattt cttgctttct tccttatcaa
    27721 tgttttctcc cggaatagta aatataatat caatttcttt ttttaggaca tagttaaatg
    27781 catttttaag aaaattataa tgcctttttt tgattgtatt tcttaaaaaa tcgtcggcag
    27841 tttccaaata aattgtattg tttttatatg ctactatttt aagaggttta aaccaggtgt
    27901 taaaacttac ttgattagta tcttctttta ctatttcaag tacttcattc cataattctt
    27961 caaaattcat aattacctcc ggtattgata aaaaaaatta acaataggtt gattaatatc
    28021 ttatatattg ataatattat gggtataaac agttgtgtat aaaaatatga acataaaagt
    28081 aattgaatat ttaatatttg taatggttga taataagttg tccattttaa atgttgataa
    28141 aaaaagttat ccacagacac ttaccatact atcatttttt atccacaaat tcaattcaat
    28201 tttaaatatt ttaaaaaata tttgcaatat atttaaaaat atagtatatt acaatagtca
    28261 acggagacgc catctactct ctgcgggaat gaggaaatgt cctcgagcaa gagtctttgc
    28321 agtcaggaga atgtccataa tttcagtgtt gacacaagta tttaatgata atataatcta
    28381 ctgatatgat aatttatgta tagattggta acaaggaggt gtttcaatga aaagaactta
    28441 tcagccgaag agaaaacaaa gaagcagtga gcatggattt ttaaagagaa tgagtacaaa
    28501 atccggaagg aatgtaataa agagaagaag ggcaaggggt agaaaaaaac tgtcagcata
    28561 aggccgcata tgtggccttt tattccatgt aatcgcaata aggtaacgaa atgataataa
    28621 ttaagaagaa taatgattac aaagctgtat ataattgcaa taattcaata tctgataata
    28681 atctcgtatt atttataaaa aaaaataatt acaactataa cagattcggc tttactacag
    28741 caaaaaaaat taaaaaagca gtacatcgaa atgttataag aagaagacta aaggaaatag
    28801 taagagcaaa cgagcattat ttagaagaag gctatgattt tgtattaatg gcaaggatta
    28861 atgccgttga ctccgattat aaaagccttg aaagctcatt cattaaactg atgaaaagaa
    28921 aaaagatata taggtacaca atttgagtaa agtattaatt tttattatac gaatatatca
    28981 gaagttttct tcaaagtctg cagtaagaca ttgcaggttt tatcccactt gctcgcaata
    29041 ttttgttgaa gcactacaaa aatacggttt tttaaaaggt tcatatttag gtatacgaag
    29101 gatattaaaa tgtcatccgt ttaaccccgg aggatatgat cctttaaaat aggagggtaa
    29161 aaatgaattt agattttata gcagtgccta tgggcatgct tgttaaactt gtatataata
    29221 tggtttctgt tgttgactct aagtatttat ctgcttattc catagcaatt ataatttcca
    29281 ccatattatt taaattaata gtattgcctt taacattaaa gcaaagtaaa tctatgaaaa
    29341 aaatgcagga actaagtcca aaaattcagg agttacagca aaagtacgga aaggatccgc
    29401 aaactttaca gagaaaacaa atggaattat ataaagaagc aaattacagt ccgttttccg
    29461 gatgtttgcc tttattaatt caattcccaa tacttattgc atttttttat gtaattcgag
    29521 aaccggtaaa atacgttttt ggagatcaag cattttatga ttccatcaac agggcttttt
    29581 tatggattaa agatttaaat gttaatgaaa atacagtatg gtttttaaag aataacgatt
    29641 ggattgtatt gacaaatgct ataaaagaag agtttagtat aagttcaagc atgcatttaa
    29701 atcaagttat ggaagtatta aatgctcaag atatcatggt taacggattg tcaatgggcg
    29761 gcatgactct tccttttttg ggatctgcac ttccggtatt ggctgtaatt acaggtatta
    29821 taacatacct taccacaaaa atgacggcgc aaccctccat aaatgaacag caagcctcaa
    29881 ctcagaaaac catgaatatg atgatgccta taatgatatt tgtattttcc atacaatttc
    29941 ctgcaggatt ggcattatat tgggttatat caaatctatt ccagttagtg caacaattta
    30001 ttgttttaaa ttcgtccaaa aatccgaagg aggaattaaa atagatgaga aatgtaacaa
    30061 ttgagaaacc taccgttgaa gaagctatag ccgcagcact taaagaactc cgcgccgaga
    30121 ataatgaggt tgaaattgaa ataatcaatg agccttcaaa aggttttttc ggtttaggca
    30181 gcaaggatgc aaaggtaaaa gttactatta ccaatggtcc tgaagagaga gcaagaaaat
    30241 ttatagacat attgcttaaa aaaatgagca tagaagctga ttatgaggtt aattattctg
    30301 acaatatttt aaatgttgac attgtaaaaa tcggagaaga cgataaagga attgttatcg
    30361 gcaaaagagg taaaaattta gatgaaattc agtttctctt gaatctaatg gtaaataagg
    30421 gaagagaaaa ctatgtgcga gtaattttca atgttgaaga ttacagagca aagagggaag
    30481 aaactttaaa aagattagct cttaaaatgg cagacaaatg cagatattac aatcgcaaag
    30541 taaaacttga gcctatgaat ccttatgaaa gaagaataat acattcaaca ttacaggacg
    30601 taaaaggtat tattacttat tccgaaggag aagagccata caggaaagta gtaatagacc
    30661 aaaaataaca aaattccaac cacttataac cctgaaaaag ggttatttta tattttcttg
    30721 gttacagtaa ttcaagatag aaaaatatgt acatttatat aaaattaagg taaaatatta
    30781 taaggtatga agacacggaa aggtaagatg aagatatgtt tagcgatact attgctgcaa
    30841 ttgcaacatt tcccggcaat gcaggaatta atataataag attaagcgga aatgatgcat
    30901 tgaatattgc atataaaatt ttcgttgata aaaataaacg gaaaccggat aatttaaagc
    30961 caagatacct tcattacggg tttattgtgg ataaagataa aaaggtaata gatgaagtac
    31021 tcttatctta catgaaggct cctaacacat ataccaggga agatattatt gaaatcaact
    31081 gtcatggggg gattatttcc gcaaaaaaaa tactggatac tgtccttcat tatggctgca
    31141 gaacagctga aagaggagaa tttacaaaaa gagcttttct taacggcaga attgatttaa
    31201 ctcaggcaga agcagtaatt gacataataa attccaagac cgaatcaagt catgaaattt
    31261 caataaaaca tctggaaggc agactttcaa aacaaattaa tgcagttata gacgaaataa
    31321 tggaattgtt ggccaacatt gaagttaata ttgattttcc cgaatatgac gaggatgaaa
    31381 taactattga aaaagttaaa gctttatgtc ataacttagt acctgagttg gataaactta
    31441 taaatacggc tgataccgga aaaataatca aggaaggtat aaaaacagta atacttggaa
    31501 aaccgaatgt aggaaaatcg tctcttatga attttctttt gaatgaaaat cgtgcaattg
    31561 ttaccgaagt accgggaaca acaagggata ctatagagga atacgtgaat attaagggcg
    31621 tgccgttaag aataatcgat actgcaggta taagagaaac cgatgatatg gtagaaagaa
    31681 taggtgttga aaaagcttta gaaaaggtaa aggaagccga ccttgtaata atgatttttg
    31741 attcatcaaa agagcttgaa aaagaggatg aaagtatatt agattatatt aaagataaaa
    31801 aggttatata tataaggaat aaaactgatt tggaaagcag gctggatttg tcttcttata
    31861 aaaaaataga agaagagatt atcaatgtat ctattttaca aaatcaaggg ctggatgata
    31921 tcattaataa aatttactct ttatttttta aaggccggtt agatataagg gatgagctta
    31981 ttattaacaa cgtaagacat aagaatttat tgataaaagc aaaaaacaac attgaagaag
    32041 tgttaaaatc aattgaaaac aatatgccca ttgattttat cgagatagat ttaatgcaag
    32101 caatggaaaa tttgggattg attgtaggta aatcggtcag cgatgattta attgacaaaa
    32161 tattcgatga attttgcata ggaaaatagg aaaggaacaa caatggaaga cagagtaaga
    32221 gaatttctcg aggagagcat tgatgtgtta gtaattggtg caggtcatgc aggatgtgaa
    32281 gcatccttgg cgactgcacg tcttggttta aaaacaataa tgctgacttt aagccttgat
    32341 tcaatagcga tgatgccatg caatcccaat atcgggggga cggcaaaagg acacttggtc
    32401 agggaaattg atgctcttgg cggagaaatg gctttaaata tagataaaac tatgatacaa
    32461 tgtaagatgt tgaaccgctc aaaaggacct gcggttcatt ccttaagagc ccaagccgac
    32521 aaaaaaaagt atcataggga aatgaaaaag gtaatagaaa atcagaaaaa tttaagggta
    32581 atacaaacag aagcaactaa gataaaagta aatgaagata agagctttaa tgtatatacc
    32641 aagcttggag catactacag ggcaagagcg gttatagtta ctacaggcac gtatttaaag
    32701 ggaagaattt atatgggtga attgaactat tcatcgggac ctgatggttt ggctcctgca
    32761 aatgaattgt ccgattcatt gatggactta ggaattgagc ttataaagtt taaaacggga
    32821 acacctgcaa gaatacacaa ggattccatt aattatgaaa aaatgacaat acagaccggt
    32881 gatgatgaaa taataccttt ctcatttatg aatgatgaaa taaacattga gcaggttccg
    32941 tgctatatta cctacactac ccaacagact catgaattaa taaggaataa tttagacagg
    33001 tcgccgcttt acggcggtgt tataaaaagt gtgggaccaa ggtactgccc atcattggaa
    33061 gataaggtag ttaaattttc agacaaggaa aaacatcaac tttttgtaga gcctgaaggt
    33121 cttgatacaa aagaaatgta tatacaggga atgtcaactt ctctgcctta cgaggttcag
    33181 attcaaatgt atcgaacaat gataggactt gaaaatgctg aaattatgag acctgcttac
    33241 gctatagaat atgatgccat cgaccctacc cagctcaagc cgagtcttga attaataagt
    33301 attaagaact tatttttcgc aggtcagata aacggaagct caggatatga agaagctgcg
    33361 gcacaaggtt tgatagccgg aataaatgca tatttaagct taaataataa agagcctttt
    33421 attttagacc gctcggaagc atatatagga gtgctgattg atgatttggt taccaaggga
    33481 acaacagagc cttacagaat gatgacatcg agagcagagt acaggcttct tctaagacag
    33541 gacaatgctg atgaaagact tacggaaaaa ggctacaaaa taggattagt aacagaagaa
    33601 agatataaaa gattcaaaga taaaagacaa agaattgaaa atgaaatcaa tagattaaaa
    33661 aatcttaaaa taacaccaaa ggctgatact aataaagctt tggaggatat gggaagcgct
    33721 gctttaaaaa ttccgacaac actttatgat ttattgagaa gaacagagct gaattatgac
    33781 aacaccttgt tcttggatcc aaagagacct gtattgagaa aagaaataaa ggattatgtg
    33841 gaaacagtta taaaatatga aggatatatt caaaagcaga tagcccaggt agagcaattt
    33901 aaaaagcttg aaaacaaaaa aattccggaa gatattaact acgatgatgt aaaaagtctc
    33961 agaattgaag caagacagaa actaaacaaa atcaggccca actccattgg tcaggcttca
    34021 agaatttctg gtgtttcgcc tgccgacata aatgttttgt taatatattt aatgacttac
    34081 aataaagaat aaaggtgagc ttgtgataaa aacattggaa gagggtttta aagagcttaa
    34141 attaccctac agcaaagata ttgaaaataa tttcattatt tacagagatt tattaaagga
    34201 atggaataaa aaaataaata ttacttccat agaaaatgat ggggaaattt atataaagca
    34261 ttttattgat agtgttcttt tgttgaatga aaataatata aatgaaaaca aaactataat
    34321 tgacgtaggt accggaggcg ggtttccggg gcttccgctt aaaattgtta ataataatta
    34381 taaaattacg cttttggaca gtttgagaaa gagaatagat tttttgtccg aagtggtaaa
    34441 agctttgaat ttaaaggatg tgaaacttat tcacggcaga gctgaagatt acggccaaaa
    34501 taaggaatat agagaatgtt atgatatttg tgtatcaaga gctgtagccc ctcttaatgt
    34561 tttgagcgaa tattgtattc cttttgtaaa agtaggagga tattttgcag catacaaaag
    34621 cgacaatatt tcccaggaaa tttcaagttc cgacaatgca attaagaaac ttggcggaga
    34681 aattaaagaa attaaaaaaa ttaatcttcc gggtactgat attataagaa aaattgttat
    34741 aatagagaaa acagacaaaa cagaaatcaa gtatccgaga aaagcaggaa agccggccaa
    34801 ggatccgtta aaataagggg gaggaaatga ttagcaatat tgttcatatc gatgtcaata
    34861 aaataattcc aaacaaaaat cagccaagaa aaacttttga tgaaaaggca ttgaaggaat
    34921 tgagtcagtc catagataat tatggaatta tacagcctat tacggtaaga aaaatttatg
    34981 atgatattta tgaaatagta gccggggaaa ggcggtataa agctgttaaa atgttaaata
    35041 tggatacaat tcccgccgtg attattgaag ccaaggaaga agaatccgct gcaatgtctt
    35101 tgattgaaaa tttgcagcgt gaagatttgc attatctgga agaagcatat gcctatgaaa
    35161 gacttataaa cgaatttgga ataagccaga cacagttggc tcaaaaattg ggcaaatctc
    35221 aatcagcaat tgcaaacaaa gtgagaattc ttaaactgcc ggagtcggtt aaagaaaaat
    35281 taagagaagg aaatttaacg gagcgccacg gaagagcctt gttaaagata aatgatgaag
    35341 aaattcttct taatattgtt gaaaaagtaa ttaaaaagga tttaaatgta agtgaaaccg
    35401 aaaaaattgt agattcaatt gctgatgagc tgaatattaa gaagaaagcg gacaagcgtt
    35461 atgtaaggaa ttttataaat tataaaatat atataaatac aataaaaaac gcttataatg
    35521 aaatagtaaa aacaggtatc gatgctaaat ttgagcagaa cgaatcagat gattacattg
    35581 aaataaaggt gaaaattcct aaaaaaagca tgtagagggt gatgatatga ccaaagtcat
    35641 aagtattttc aatcaaaaag gcggtgtggg gaagacgaca accaatgtta atttatgtac
    35701 tgcattggcg ttaaaaggaa agaaagtact tagcattgat atagaccctc aaggaaacac
    35761 aaccagcgga ttcgggttta acaagaataa actggaatat acaatttaca atatattgat
    35821 tgaagattac gacatcaaca aaactatttt caagacggaa atagaaaact tagatattat
    35881 tcccgcaaat attcagcttg caggtgctga agttgagctt ataaatacta aattcaggga
    35941 aaaaacatta agggaaaaaa taaatttaat tgaaaacaaa tatgatttta ttatggtgga
    36001 ttgtccgccc tccttggggc tattgtcatt aaatgctctt actgcatcca ataccgtact
    36061 tataccaata caatgcgaat actattcttt ggaaggtgta ggacagctta ttgatactgt
    36121 taagcttgta aagaaaaatt taaatcctaa gcttgaaatc gaaggcgtta ttttgaatat
    36181 gtttgacggc aggacgaatc tttcgataca agttgttgag gaagtcaaaa aatattttaa
    36241 ggataaagta tatagaaccg taataccgag aagtataagg cttgccgaag caccgagctt
    36301 tggacagcct ataatgctat atgacgataa atcaaaagga agcattgcat atataaaatt
    36361 atctgaagaa tttattaaaa ataattgagg agagaaaata gtatggctgc aaagagaaaa
    36421 gcattaggca aaggcttatc ggcattaata cccgaagatt tagaaaagga agattatgaa
    36481 aaaatccatg aaatagatat tgatttgatt tccccaaacc caaatcagcc ccgcaaactt
    36541 tttgaacagg ataagttaga tgaacttacg gaatccataa aaaaatatgg tgttat
//
LOCUS       DAOPUE010000007        36190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1113_length_36190_cov_9.864148, whole genome shotgun sequence.
ACCESSION   DAOPUE010000007 DAOPUE010000000
VERSION     DAOPUE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 36190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36190
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_36190_cov_9.864148"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..3727
                     /locus_tag="PKH42_01420"
     CDS             320..3727
                     /locus_tag="PKH42_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOA19116.1"
                     /translation="MSLKIIAGRANTGKSSFIYDEIKTQSKNSKAKLILIVPELMTYQ
                     AESNIIERFDLPGIMNIEILSFKRLERKILEEVGGLKMQDLSVFGKIMLLKKIFEENK
                     DKLKVYGNSYKQSGFLKEFEELITEFKQNLVEGQSLVQAAQDMDDEILIRKLSDIQLI
                     YSEFIERTKNKYFDEEDKTNLFISLIKESAYIRNSNIWIDGFESFNRQRLHLIKNLCD
                     YSKSVTLSLNIDSSYLDNLEEKDDFEAFKIIYDTYHTLKNFDTDIEIISLSENKSPST
                     EIKAIEKNIFSLNIEEYENNTDKINIFSSLNPYSETQKTAANIISLVRDENLRWRDIK
                     VAVGNMEVYETNIKRIFSQFEIPFFLDVKRDILNNPLSKYILSILDMFIWNFKHDNVF
                     EYLKTGFSPLNINQVQYLENYALQFGIEGDKWFREFKSFDKDGNQITGEKNNIERYRK
                     LFAEDFEVERKKIHNLKTANEITKLIFKYLDKHKIKEKMEKAVKEFNNAQKYEEASEY
                     SQCWNYVMEIFEQLLLIGQDTEISLNDYRKMLEAGLGEVEVSIIPPAIDKVEVGTIDR
                     IAVTKPKALFIIGANDSNLDTKNTDKGLLLNDERDILLENDVRLTKGADYDTFKEKHM
                     LYKMFSSPSHSLSVSYPLGLESGESLQPSLYIDILKKIFVSVKESSDLSISDELDLVS
                     NYSGTYDYFVEKMRNFIDGYETDNIWKDVYAWYEREEKEIFDIMVKGLNYKNAMNSLE
                     PDIVKRIYENNMSMTVSKLESYAECQFKYFMENVLKPRERAYLKIEYYDLGNIYHSIV
                     EKFINKISEDKEDIIILDRTKAEDEAVKITDDVLLEQADKLTAIDANHRNKYMKEKIK
                     RVMKRTCWTIVRQLNSSEFRPKFTELQIDLVDENEEKAKKGVYLPPIEIPVETDKIKE
                     VLKLRGKIDRVDVFENNDKLYISIIDYKSSPNDIDLEDAQEGIQVQLIMYLKALLDKG
                     EELFGKKPCIGGVFYYYISDPVYKDKNKEKEPEEEIFKSLKLKGYVLKDKEVIKYMDK
                     NIGSTSSIIPAALKSDGEIRDDRTKALTEESFNNLIDTVYNKCKDMTKSIISGNININ
                     PYKKIDGKTPCKYCEYISICQFDKTLGNNFRLIGNQFKNLSNLYTEH"
     gene            3742..7461
                     /gene="addA"
                     /locus_tag="PKH42_01425"
     CDS             3742..7461
                     /gene="addA"
                     /locus_tag="PKH42_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HOA19117.1"
                     /translation="MNWTKEQKRIIDLKNSNILVSAAAGSGKTAVMVERIISLIKSGE
                     DIDRLLVVTFTKAAAAGMKQKIQKSLLNAYQNKDGNIKHLRKQLSLLNRSMITTIDSF
                     CLDVVKKNFHLIDIDPNFRVGDKSELSILLQDSIDEVLEEEYEKINGNENFRKLVEGF
                     TGNRGDDELSEIIQSTYRFILSFPDPFVWLSQSVDKLNIKGEELKNSSWLKEIKSYIM
                     LLLDGAKGYINTALEICNEPKGPRLYLDTLKKDMAIVDNLVNLLNNNMEEFMEAVKGF
                     KAPKAPPFKEKDNPDVDIEKQIEVGGSKNNNSMRAGYKSIIASIKELLPYDLDYDKYS
                     LEINEMYGSIAALKDLIIKTDKIYKEKKNEKSIADFNDLEHFALNILRSKNDKGVFEP
                     SEAAEYYRKKYNYIFIDEYQDSNSLQEAIIEQIKRENNLFMVGDVKQSIYRFRLADPS
                     IFNNKYTTYTMDREDLSNNIVDRTIDLNKNFRSRDEILKAVNFIFKNIMTKELGEVEY
                     NEKAALNTGSEFLTHNPVELNIIYKNTSLENQEGEAEAGEVIKSEAEIEIESMETAEL
                     EALFAAKRIKELIKEETCIPGKTKGKKIEYKDIVILLRSVVNWAGIFEERFNKEDIPF
                     YYDGGKGYYDALEVRILVNLLRLIDNIRQDIPLLSVMRSPIGNFTTEELLEIRLKHPN
                     EKHFIDALNKYILCAKEEEESFPLADKLKNFIEKIYDWSYRSRYSHLHDLIWDILMES
                     DYYNFAGALPNGRMRQANLRMLADLAYDFEKTSMKGLFKFLRYIEKIDKGYDDRGQAK
                     TLGENDNVVRLMSIHKSKGLEFPVVILCGLNKQFNLQDTKNKILLHKDYGIAPMYVNV
                     NERFERETLARTAIKMRIKKENISEEMRVLYVAMTRAIDRLIMAGTVSNLEQKEKNWR
                     RGHSQYFVYKGLSYLDWICSCLFEKINFDYFNEIMAQGQWKNWILNLITRADLSLSIR
                     DEINFKESNIRAMNEYKDMKNSPYFEEIGRRLGFKYPYMSSVNVPTKLSVTELKNLKG
                     KEYAKLRYKIPTLVDIFEYNEVTDKLIFNKEITGAEIGTLLHFVMEHLDLKGNLKKSG
                     IISTIKQMENKKLLTEAESKIAISTYAEKIEEFFQSKIGKRIIDSSAVYREAPFVLRK
                     KANDISGSLNKDDLILVQGIIDCYFIEDGEAVIVDYKTDKIDETKEIEFQINNLKSEY
                     KDQIELYKEAVEKITSKNVKECYLYLFSIGKEVKI"
     gene            7551..8201
                     /gene="deoC"
                     /locus_tag="PKH42_01430"
     CDS             7551..8201
                     /gene="deoC"
                     /locus_tag="PKH42_01430"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOA19118.1"
                     /translation="MEYLELMKYVDHTLLKPYATWEEIRVLCDEAINYQTASVCIPPT
                     YVKRVHDTYQHKLNICTVVGFPLGYSTTEAKVAECVQALNDGADEIDMVINISDVKNK
                     DYKKVEDEIRTIKETVGEHILKVIVETCYLTNDEKIEMCHAVTKASADYIKTSTGFGT
                     GGATMEDVQLFKKYIGQNVRIKASGGIRTLNDMEAFINAGCSRIGTSSAIKLINNA"
     gene            8245..9429
                     /locus_tag="PKH42_01435"
     CDS             8245..9429
                     /locus_tag="PKH42_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA19119.1"
                     /translation="MKLWRKLAIVALVFIMIFAFSACANNQPEPPEEPETPETPETPE
                     TPEEPEKPAANVFELALITDVGTIDDKSFNQGAWEGVEAYAKEHNITYKYYQPAEQSD
                     TAYIDSIGLAVKAGAKVIVCPGFLFEVPIHTVQTMYPEVNFVILDGAPHAGDWVPDIA
                     DNTYSIFYAEEEAGFLAGYSIVKEGYTKLGFMGGMAVPAVVRFGYGFVQGAEYAAHEM
                     GLTDPVQIKYTYVGNFDATPENQAKAASWYNEGTEVIFACGGAVGNSVMKAAEAAGTK
                     VIGVDVDQSAESETVITSSMKNLSKSVYDALTAYYAGNFPGGTSVTLDATVEGVQLPM
                     DNSRFETFTQADYDAIYGKITAKEIEIMNDAEVVEDAGKPAEEVTAADIPVSKVQVEV
                     IQ"
     assembly_gap    9436..9535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9587..11122
                     /locus_tag="PKH42_01440"
     CDS             9587..11122
                     /locus_tag="PKH42_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19120.1"
                     /translation="MEYVIEMNNITKIFGNFKALDNVTFNLRKGEVHALLGENGAGKS
                     TLMSVLFGLYQAEKGSIKINNKTVKINNPNDASSLKIGMVHQHFKLVHNFTVLESIIL
                     GRETVNKGILKKDDARKKVMELSERYKLRIDPDAYISDITVGMQQRVEILKMLYSDSE
                     ILIFDEPTAVLTPQEIEELMKIMKELVKEGKSIVFISHKLNEIKEAADRCTVLRKGKY
                     IGTVDVKTTTKEQMSEMMVGRKISFNIEKDDIETGEAVLEVKNLTVKAKNSKKNMVNN
                     VSFKVKAGEILCIAGIDGNGQSELVYGITGLIPINEGQIFFKGKDITKYSVRKKSING
                     MSHIPEDRHKHGVILDFDLKQNAVLQTYFQPRFQKRGFIKFNAVSEYAKKLIKQYDIR
                     SPRDEDSIVRNMSGGNQQKLIVARELDKDPDIVIAVQPTRGLDIGATEYIHKQLIAQR
                     DQGKAVLLVSLELDEVMDVSDRILVMYEGEIVGDLNPKEITVEELGLYMAGSKRSDRY
                     EKN"
     gene            11109..12296
                     /locus_tag="PKH42_01445"
     CDS             11109..12296
                     /locus_tag="PKH42_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19121.1"
                     /translation="MRRTRLLQYEGFTSFLSSLMAIVAGLLVGLIILLISNSEDAFPA
                     FLTILKGGFVDGSRGLAQVIYFAIPIIMTGLSVGFAFKTGLFNIGTPGQFTLGAFVAI
                     YIGIKWTFVPPEIHWLVALLGAMVAGAVWGSGPGILKAFFNVNEVITSIMMNYIGMYL
                     VNMSIVKTVYDSLKNQTKPVLKSAVLPKAGMDKLFNTSNVSIGIFIVILAVIVIYIIL
                     QKTTFGYELKACGSNKDASKYAGINEKRSIVLSMVIAGALSGLGGGLLYLSGSGKYIH
                     VLDVLAPEGFSGISVSLLGMSHPIGIFLAGLFIAHLEVGGFNIQLYNFVPEVIDMIIA
                     VIIYCGAFALLFKQIISKFIIHDTDEDIGKSVQAADIPASYESSVVNAETEEKTEQRE
                     DKK"
     gene            12293..13246
                     /locus_tag="PKH42_01450"
     CDS             12293..13246
                     /locus_tag="PKH42_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19122.1"
                     /translation="MSTVYFLAQQTMYFAIPLLIVALGGMFSERSGIVNIALEGIMVV
                     GAFFGIGFIHIFQDSMSGQSLLLLAVLVSALTGAIFSLFHAFASINLKANQVISGTAL
                     NMFAPAFAIYTARMIQGVQQVQFMDTFYIRSVPILGDIPILGPIMFKNAYITTYIGLL
                     IALIAWFIINHTRFGLRLRACGEHPQAADSVGINVYHIRYAGVIISGILAGIGGLTFV
                     VPTSTNFNATVAGYGFLALAVLIFGQWRTKKIFVAAFFFGIMKTFASAYSGIPFLKGL
                     PISNEVYKMIPYIATLIVLTFSSKKSQAPRAEGIPYDKGSR"
     gene            13263..14774
                     /locus_tag="PKH42_01455"
     CDS             13263..14774
                     /locus_tag="PKH42_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOA19123.1"
                     /translation="MFADNKIWIGKSDKKVYLEPKMANRHGLVSGATGTGKTITLKVL
                     AESFSDLGVPVFIADIKGDLASIAIPGTDNENMQERLNRLEINNFIYKGYPVQFWDVY
                     GEGGIPVRTTVTEMGPLMLSRILNLNDTQTGVLNIVFRVADERGLLLLDLKDLRAMVQ
                     YVGDNSKDFRMKYGNITAQSIGAILRSLMTLEDQGGEFFFGEPELDIFDWIKTSADGR
                     GYINVLHCAKLFLNPLLYSTFLLWMLSELYENLPEEGDLEKPKIVFFFDEAHLLFEDA
                     PKQLLDKIELVVRLIRSKGVGVYFVTQNPIDVPNDILGQLGNRIQHALRAYTPAERKK
                     VVTAAETFRENPEFNTVEAITELGTGEALISCLDEKGIPSVVERAFILPPQSRFGTID
                     DMLRNQIIAGTPIYKKYYKSVDRESAYEILLAKTERKYQEELLETERLEEEKRLKLEA
                     KERERQQKEFERARKRASSKTTAIDRVANSAMNAIGREVGRSLIRGVLGTFKR"
     gene            14774..15118
                     /locus_tag="PKH42_01460"
     CDS             14774..15118
                     /locus_tag="PKH42_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOA19124.1"
                     /translation="MYNKNKGFEYEKIAEKYLQNNNYKILEVNFISKFGEIDIIAEKD
                     NRLHFIEVKGRKNIKHGYPKEAVTFSKQKRLRSAAKYYFMLKGKDDYLCQFDVIEIIL
                     ESRDINHIKNAF"
     assembly_gap    15165..15264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15336..16649
                     /gene="mtaB"
                     /locus_tag="PKH42_01465"
     CDS             15336..16649
                     /gene="mtaB"
                     /locus_tag="PKH42_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOA19125.1"
                     /translation="MNKDTSKVYTVSFITLGCKVNQFETEAMTEILERDGFKVLPPNE
                     VSDIYIINTCAVTKESERKSRQFINKAKRINPDALVVAVGCSVQLNSEKIKKETQADI
                     IIGTKHKGNIGKLIKNKLNDIKQECKIDDFRGRESFEELKISNVYDKTRVNIKIQDGC
                     SQFCSYCIIPYLRGPIRSRNHEDIIGEVKRITKNGYKEIVLNGIHISSYGKELKENYY
                     LIDLIEDINKIEGIERIRLGSIEPNLINEDFMKRYAVLDKACDHFHLSLQSGSDSVLK
                     RMNRKYSTNEYKNNVELIRNYMPDAGITTDIIVGFPGETDEEFNETLEFVKKIKFSRI
                     HVFKYSVREGTKAAEIKPQVNEQVKADRSKVLIELGKNISKEFMKKFIGRKLSVLIET
                     KKNENIYEGYTTNYLKVMLKSDINVKNEIINIHVTGIRNDYLLGE"
     gene            16672..17013
                     /locus_tag="PKH42_01470"
     CDS             16672..17013
                     /locus_tag="PKH42_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOA19126.1"
                     /translation="MDCIFCKIANKEIPSQFVFENEYVVAFRDLAPQAPVHILAVPKI
                     HITSMNEITKENSHIVAEIFEAINKITESEGIRDSGYRVISNCGRDGCQSVQHLHFHI
                     LGGKKLSESLA"
     gene            17121..17306
                     /gene="rpsU"
                     /locus_tag="PKH42_01475"
     CDS             17121..17306
                     /gene="rpsU"
                     /locus_tag="PKH42_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOA19127.1"
                     /translation="MTEVKVRENESIDNALRRFKRQCALTGVMSEVRKREHYEKPSVK
                     RKKKAEAARRKKNKKAR"
     gene            17318..17761
                     /locus_tag="PKH42_01480"
     CDS             17318..17761
                     /locus_tag="PKH42_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOA19128.1"
                     /translation="MPLKEKLMEDLKESMKSKNKVKKDTVTMVRAAIKQKEVDERIEL
                     SDAEIIDIIAKQVKQKKDSIPDFEKGNRQDLVDLTNEEIKILLEYLPPQLSDEDLESI
                     VKEAIEKTGAQSKKDMGKLMAFIMPKVKGKADGKHVNEIVAKYIK"
     gene            17925..18206
                     /locus_tag="PKH42_01485"
     CDS             17925..18206
                     /locus_tag="PKH42_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HOA19129.1"
                     /translation="MSKFNNFRSKLADELEIPNNALSDNFELRMHGNKKVIIENHLGI
                     KVYDENMVCIKTNDQDITIKGSKFKIEEINNYKVIVKGNIKEIIFSMKE"
     gene            18209..19414
                     /locus_tag="PKH42_01490"
     CDS             18209..19414
                     /locus_tag="PKH42_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOA19130.1"
                     /translation="MLIFKLMYLIRGYVIIRLDAKNSEKLLNILQRRNIKVWDVEKKG
                     KGIKFKISYEDYLKNSTLINDIVKPESKRGFLLKLNKVKLRKGFSIGLLILIITLYLL
                     TSMVWDVEVIGTNQSNTNEILALLEKNHIKIPVSISRIEAKQLETLIHKNFKYKFVDV
                     FVEGSKLIIFVREREPDPAEIKSNEPSSIISKKNAIINKVIAKSGQPVVKEGDVVYEG
                     QTLIMGIVKNKNSDEFMMVPSDGIIYGKTYYNFEMKEEKIKSMNVPTNKSKIVYYLKI
                     NENSIKILGNKDPYQNYEYRENEIKVPILSNIADIGFIKGVYYEEVIKEIEIDKNTAQ
                     NKMKIAMYDELLKRCDDDSRILKSNINFSEDEKFYYLIAQIEVIEDIGERVRIFSTEE
                     EKTDETKED"
     gene            19414..20373
                     /locus_tag="PKH42_01495"
     CDS             19414..20373
                     /locus_tag="PKH42_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOA19131.1"
                     /translation="MELFEQNVLIANEDELIKLLGIHDKNIKIIKSYYDVNILVRNGA
                     LKITGEKENSEAVSKIIKDILNTIRTEGDISDQKVIYLIEANKSNSQIDYNQILKEVI
                     VTSASGRIIKPKTVGQKRYVDMINSKDVTFGIGPAGTGKTYLAVACAVNAFRKKEIER
                     IILTRPAVEAGEKLGFLPGDLQDKVDPYLRPLYDALFDIMGIDNFMKNVEKQLIEVAP
                     LAYMRGRTLDSSFIILDEAQNTTNEQMKMFLTRLGYGSKAVITGDITQIDLPEGKKSG
                     LKSVINILKDVEDIGFMFFHTTDVVRHKLVQDIVKAYEKYEVK"
     gene            20373..20819
                     /gene="ybeY"
                     /locus_tag="PKH42_01500"
     CDS             20373..20819
                     /gene="ybeY"
                     /locus_tag="PKH42_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOA19132.1"
                     /translation="MIKVFFDDRQDVMEITKDNEDAIKNAVDAVLTAEGLNGDFEVSV
                     SFVTNEEITELNREYRNVDSETDVLSFPMNEEFDGVNILGDIVISTQKIIEQANDFNH
                     SMEREMCYLTVHSMLHLLGYDHMNKEEKNKMRAKEKEIMKKLKIFK"
     gene            20839..21903
                     /locus_tag="PKH42_01505"
     CDS             20839..21903
                     /locus_tag="PKH42_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOA19133.1"
                     /translation="MKTKIAVLIICLLFINACAKETVTPEQAEPEINEEEPEEKPEVD
                     KEEIEEEIDPNKVLSPLDGLRYYPEELSKRPVAVSIDNHHRARWQAGISQAEIVYEVE
                     VEHPFTRYLCIFLAHEPEMIGPVRSARPYMIYYALENDGIFVHVGGSEDAFSEIKRLK
                     VADVDGLYSGAMWRYNDTGKYAPHNVYTTLESIRNEAKAYGYRDEGNFEGYLFNEKDV
                     KLSENFETTPSKEVNIVYNYYNTTDYIFNEENNLYLRFKDDEEHIDELDKKQLTAKNI
                     IAVETSKSILDNEGRLFLGTIGKGKGIYITNGESIPIIWEKTSEKDRLKFYSGDEELM
                     LNPGNTWIQVVNSLENITLR"
     gene            21945..22337
                     /gene="cdd"
                     /locus_tag="PKH42_01510"
     CDS             21945..22337
                     /gene="cdd"
                     /locus_tag="PKH42_01510"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOA19134.1"
                     /translation="MEDKLLIKIAMEAREASYSPYSNYKVGAAVYTKSGKVFTGCNIE
                     SASFTPTICAERVAVSKCISEGYRDIYKIAVVGSEIKTSFPCGVCRQFLAEFGKDIKV
                     ICARNIDSYDVYTLEELLPNSFGPDDLA"
     gene            22350..23237
                     /gene="era"
                     /locus_tag="PKH42_01515"
     CDS             22350..23237
                     /gene="era"
                     /locus_tag="PKH42_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOA19135.1"
                     /translation="MFKSGFVTIIGRPNVGKSTLMNNMLGEKISIMSDKPQTTRNKIR
                     TVYTDDEAQIIFIDTPGIHKPKNQLGEFMNTEVDSALEEMDVLIMITDEFNKVGPGDE
                     FIIEKIKDIKAKKILIINKIDKFDKEAALRIAAEFDKYKVFDDIVPISALNNVGVDAL
                     VKLIIKYLKPGPMYFPADYITDRPERFIVSEIIREKALMYLEDEVPHGIAVEIEEMKE
                     RKDRDLVDIRANILCEKKSHKSIIIGKEGRKIKGIGKSAREEIERLLGSQVNLQLFVK
                     ISENWRDDSYRLKTLGYDK"
     gene            23234..23947
                     /gene="recO"
                     /locus_tag="PKH42_01520"
     CDS             23234..23947
                     /gene="recO"
                     /locus_tag="PKH42_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOA19136.1"
                     /translation="MIQEEILILKEISYKENDKILHALSRTRGKIRIISKGSKKSSSH
                     LINASQIFAYSTCTLNVSKDMYILTSAKLLDNFYNLNKNMAAYYNASYILELISYVAQ
                     ENEVDSRVFDMTVSVISHLSNCTYEHEKLTAAYELKLAAMLGYKPDFLHCLRCGSSIN
                     DESKFSVTEGGVFCSKCVNFGNGINVTYNEILIMDKILKSKFDNIGFIEIPKTIMNLI
                     RKFLFYYIGKDNFTTLKLL"
     gene            23964..24566
                     /locus_tag="PKH42_01525"
     CDS             23964..24566
                     /locus_tag="PKH42_01525"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOA19137.1"
                     /translation="MENDTLNKIDEIIKRTNTDYGTAKEALEAANGDVLEAIILIENQ
                     QKGSTGSFRGEQIMAQLKDILAKGNATKLTIKKDNEVIVNIPITAGLVSAFIAPFLSA
                     AGITVALLAKCSVEITQTDGKIIDLGQKVDEGIDAVKDAMHDIKKGAQNLKDDLRTGS
                     KNIKDDIKTGAENVKDNIKAEAENMKDDLKNSFDRNNVHF"
     misc_binding    24610..24787
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24893..26287
                     /locus_tag="PKH42_01530"
     CDS             24893..26287
                     /locus_tag="PKH42_01530"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOA19138.1"
                     /translation="MAKIEKNSLEKVVSLCKSRGIIFPGSEIYGGLANTWDYGPLGVE
                     IKNNIKRAWWKKFIQESPYNVGLDAAILMNPTTWVASGHVGGFSDPLMDCKECKERFR
                     ADKLIEDFMKEIDDVQVVDGWTNDEMEAYVAEKKVKCPNCGAHNFTTIRKFNLMFKTF
                     QGVTEDSQSELYLRPETAQGIFVNFKNVQRSMRKKIPFGIGQIGKSFRNEITPGNFTF
                     RTREFEQMELEFFCKPGEDMEWFYYWKEFCMNWLLNLGMNKEKLRFRDHEPAELSHYS
                     KGTTDIEYLFHFGWGELWGIANRTDFDLKQHINTSGEDLYYQDPITNEKYIPYCVEPS
                     LGADRVMLAILVDAYNEETLEDGTDRVVLKLHPALSPYKAAIFPLTKKLNEQAIELYQ
                     MLNKHFNVDYDDAGSIGKRYRRHDEIGTPYCITFDFDSLEDNTVTVRDRDTMEQVRIK
                     IDDLVTFIDEKTSF"
     gene            26354..27355
                     /locus_tag="PKH42_01535"
     CDS             26354..27355
                     /locus_tag="PKH42_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOA19139.1"
                     /translation="MNIREKYEQFEIEHLSEYASKAALSKGRMLYEPKCEIRTDFQRD
                     RDRILHSKAFRRLKHKTQVFLSPEGDHYRTRLTHTLEVSQISRTIARSLNLNEDLTEA
                     IALGHDLGHTPFGHTGERILKELCNGFKHNEQSLRVVDFLEVRNERQGLNLTFEVRDG
                     ILNHPSGYKPATLEGQIVSISDRIAYINHDIDDAVRGHIITKIPDKFLKNLGSSHGQR
                     INTMIMDIIQNSENKNKIVMSDEVAELTDELRNFLFENVYYNKVAKSEEDKTMFIVEK
                     IFEYYTNNFEQLPEFYINIYNNNNFSKGEIIKDYIAGMTDRYAMKVFEELYIPKPWI"
     gene            27487..29241
                     /gene="dnaG"
                     /locus_tag="PKH42_01540"
     CDS             27487..29241
                     /gene="dnaG"
                     /locus_tag="PKH42_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOA19140.1"
                     /translation="MPYRLDSDVIQRILEQNNIVDIIEEYLPLKKAGANYSTTCPFHK
                     EKTPSFIVSPDKQIFHCFGCGESGDSISFLTKYKNYTFVEAAEYLANRAGIILEEINS
                     SRINNNKQTADMLYKINRDAAAYFYKNLRKSPNAIKYLFSRNIKSDVIKSFGIGYAID
                     EWDNLLNYLKNEGYNEEDILKTGLIIWNDKKNTYFDRFRNRIIFPIFDIKKRIIGFGG
                     RVIDDSMPKYLNSPESLIFNKGYNLYGIHIAKENIRNNSFILVEGYMDVIKMHAHGYK
                     TAVAALGTSFTENQAKLLKRYSRDFYIAFDSDEAGQKASLKAINMLKRNNLNAKIVIM
                     KDAKDPDEYLSKYGKPAFDKLIENSLDYYSFLEFHFKEILENSNKVEYINNLFDNIVN
                     VNSEIERELIFDKLSRKVGVSKESIIKEFNKRSTKQKTLDKTAIPAVKPQVAKITTCH
                     EEELIKLILLDNDFALKLSEIVNEDTFKDLKYYNILKELYECRINDNSIDSKSLNKII
                     KDDMNVNLQFDNVDYDNLEALFQDCMKRLKIKYYKEKRSILNNILKQSDNSLNRKEIM
                     NEIYCLAKKIKSTKEEVN"
     gene            29244..30413
                     /gene="rpoD"
                     /locus_tag="PKH42_01545"
     CDS             29244..30413
                     /gene="rpoD"
                     /locus_tag="PKH42_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOA19141.1"
                     /translation="MSENKNISVISEEEYAKNEEMLQKIDSIKNKLIEKGKRNGFITY
                     KEVLRSFEKLDINPEFIDDFYKQAEDNDLEILGFQDDMIPEKVDEEDEIDSKEDADFF
                     VSQSDDDILKGVNIDDPVRMYLKEIGKVSLLSAQEEVELAKRMEAGDEEAKKQLAEAN
                     LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGFKFSTYATWWIRQAITR
                     AIADQARTIRIPVHMVETINKLIRIKRQLLQELGRDPTPEEIALEMEMEPEKVREILK
                     IAQEPVSLETPIGEEEDSHLGDFIPDDEVQAPSDVATFTLLREQLSNVLHTLTDREQK
                     VLRLRFGLDDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLKDYLE"
     gene            30675..31349
                     /locus_tag="PKH42_01550"
     CDS             30675..31349
                     /locus_tag="PKH42_01550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA19142.1"
                     /translation="MNRLECIVSMVDKCNTAADIGTDHGYVAEMLLKKGICNKIIATD
                     LNKGPLYRAREHLSEVKLSDKCDFRLGDGLTVLNEGEADTIIVAGMGGDLISEIIERS
                     KHIAESAKQLILQPMTKVDNLRRYLCNNGFEIIDENIVKEYNHFYFVIKTKKGFTKYM
                     DDIYYEISKVLTDKRDTVMIEYINKVIDTNKKIISSLEKADNVAYNEKIEELKRKNKK
                     IRELIR"
     gene            31346..32119
                     /locus_tag="PKH42_01555"
     CDS             31346..32119
                     /locus_tag="PKH42_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOA19143.1"
                     /translation="MNLNNIINLLNEEVKLKEHESWDNSGLQLGSMEVNINKIMLTLD
                     INKEAIEYAVQHKVDLIITHHPFFFGSIRKIDFDTYDGEIIKMLIKENINVYSMHTSY
                     DMADKGVTYSLAKRLNILNYQVLHPINIDNSGYGGIGDVAPVNIIEYTKLVKEALGAE
                     YVKLYCNGEKTVRRVAFCGGSGGEFIEDAIRQKADLYITGDIKYHQAQEAVKNNLCII
                     DAGHYNTEIYSMETLMEVLEKTGLEIINFKKNITAEKII"
     gene            32128..34056
                     /locus_tag="PKH42_01560"
     CDS             32128..34056
                     /locus_tag="PKH42_01560"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOA19144.1"
                     /translation="MDKNFEYIRVASAVPSIRVADPNYNAIKIKEIILKAEKEKVRVL
                     VFPELCLTGYTCQDLFSQNTLINKCEEALSLLLEETKYLNMLIAVGMPVKADTKLFNC
                     AVIFKEGKILGVVPKTYIPNYKEFYEMRWFVPASYRLSDIVTLCEQEVIFSENLLFKD
                     DISSLCVGVDICEDLWATIPPSSYHALQGANLILNLSASSETSGKADYRRDLVKMQSS
                     KCITSYVYTSSGEEESTTDVVFSGHCIIAEDGKILKEDNLCDGFIYSDIDIEKINNEK
                     RSLNVYNSNIERKNYKYVNFNLGYSENLKLERFVEPRPFIPARPEDRDKSFSNILKIQ
                     AAGLSQRMKKIGSKKAILGISGGLDSTLALIVAVEAIKKLKLPLTNIITVIMPGFGTS
                     ERTYKNAHILIDELGVTLREISIKDACMQHYKDIGHDINNHDVTYENSQARERTQILM
                     DIANQEGGLVVGTGDLSELALGWCTYNGDHMSMYSVNSGVPKTLIKYMVKWYADSKTS
                     GEMQKTLLDICNTPVSPELLPPDKEGNIRQLTENSVGSYELNDFFLYNMMKNGYGPEK
                     MYYLAKIAFKEEYDEQVILETLKNFYKRFFTQQFKRSCMPDGVKVGSVSLSPRGDWKM
                     PSDASYELWMKICENIKK"
     gene            34112..34529
                     /gene="rnpB"
                     /locus_tag="PKH42_01565"
     ncRNA           34112..34529
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKH42_01565"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            34610..34771
                     /locus_tag="PKH42_01570"
     CDS             34610..34771
                     /locus_tag="PKH42_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19145.1"
                     /translation="MNKISTGNIKRDDDMFCRVVPWFLLGVGFGRCSERRDVLGARGR
                     FDPCIRRRR"
     gene            complement(34861..36084)
                     /locus_tag="PKH42_01575"
     CDS             complement(34861..36084)
                     /locus_tag="PKH42_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA19146.1"
                     /translation="MKLHIYSLIMFLNSYLTGLLTPVLSLALIDKGASLSNLSIILGL
                     YALSVILLELPSGIMADVFGRKKTFVLSLVLFTLSFSLLLISKVFIVLCIVMILYGLG
                     RALGSGSFDALFIDYYIDNFGKDKLHNITTRLSVLDALGLSAGALSGGFFPDISNNLL
                     PSIGTYDLNIIVRIVLTIAVIIISVLFISEKNHHDKKEHKSIKQHVKNSSDFVRKNTT
                     ILFIFISVFSTGIFLSALETYWQPHFITLLPDEGSSGLLGLMAFLYLGAALVGSILSN
                     KIMSKYKFNIRKMYLIMRSLLALFLILTALQTNIPLFIAYYASIYLMFGMANIPEDVI
                     LNSETPNQIRASVLSVKSFTIQIGGLSGSLMYSILIRFISIPAIWVIAASIILLTIAL
                     IAKKFIAAEKARNKI"
BASE COUNT        13720 a         4862 c         7232 g        10176 t
ORIGIN      
        1 actgatttgc ggatatataa ttgtcaattt aagcatttcg gtattgttat agctgaatgt
       61 ttttctgtat tcatacatgt ttatttctgc attatagcaa taatttttca tttcataacc
      121 ctccttgaat tataatatgt gagaattatt ttatggttca aagattgact aaccctattt
      181 ttatagtgat ggggatacgc tccatattcg ggaatgcccg tctggcagga ggaatgtccc
      241 caatcggaat ttaattgcca taaacgaaaa atccttgtat aattaagaaa atacatttcc
      301 gtatatagag gcataggata tgagtttaaa aataatagca ggaagagcaa acacgggaaa
      361 atcatcattc atttatgatg aaattaaaac acaatcaaaa aattctaaag ccaagttaat
      421 acttattgtg cctgaactta tgacctatca agctgaaagc aacattattg agagatttga
      481 tttgcccggc attatgaata ttgagatttt aagctttaaa agacttgaga ggaagatact
      541 tgaagaagtt ggaggcctga aaatgcagga tttaagtgtt ttcggcaaaa tcatgcttct
      601 aaaaaaaata tttgaagaaa ataaggacaa acttaaagtt tatggcaatt catacaagca
      661 aagcggattt ttaaaagaat ttgaagaatt gataactgaa ttcaaacaaa acttagttga
      721 aggccagtca ttagttcaag cagctcaaga tatggacgat gaaattctaa tacgaaaatt
      781 atcggatatt caattgattt attctgaatt tatagaaaga accaaaaata agtattttga
      841 tgaagaagat aaaacaaatc tttttatttc tctgattaaa gaatcagcat atataagaaa
      901 ctctaatata tggattgacg gtttcgaaag ttttaaccgc caaagacttc atttaattaa
      961 gaacctttgt gattattcaa aatccgtaac attatcatta aatatcgatt cttcttattt
     1021 agataattta gaggaaaagg acgattttga agcctttaaa attatttatg atacatacca
     1081 tactttaaaa aattttgata ctgatataga aattatatcc ctaagcgaaa acaaatcacc
     1141 ttcaactgaa ataaaagcaa ttgaaaaaaa catattttct ttaaatatcg aagaatatga
     1201 aaataataca gataaaataa atattttctc ttctttaaat ccatattcgg aaacacaaaa
     1261 aacagcagct aatattatat ccttggttcg ggacgaaaat ttaagatgga gagatataaa
     1321 ggttgcagtt gggaatatgg aagtttacga aacaaatata aaaagaattt tttctcaatt
     1381 tgaaatacca tttttcttgg atgtaaaaag agatatttta aacaaccctt taagcaagta
     1441 tatactttca attcttgata tgtttatttg gaactttaaa catgacaatg tttttgaata
     1501 tttaaaaaca ggattttcac ctttaaacat taaccaagtt caatatttgg aaaattatgc
     1561 gctgcagttt ggtattgagg gagataaatg gtttagagaa tttaagtctt ttgataagga
     1621 tggcaatcaa attactggtg aaaagaataa cattgagcgg tacagaaaat tatttgccga
     1681 agattttgaa gttgaaagaa aaaaaattca taatcttaaa actgccaatg aaattacaaa
     1741 actaatattc aaatatttag acaaacacaa gataaaagaa aaaatggaga aggctgtcaa
     1801 agaatttaat aatgctcaaa aatatgagga agcaagcgaa tattctcagt gctggaacta
     1861 tgtaatggaa atttttgagc agcttttatt gattgggcag gataccgaaa tttctttaaa
     1921 tgactataga aaaatgttgg aagcaggact tggagaagtt gaggtaagta taattccgcc
     1981 tgctatcgac aaggttgaag tgggaacaat tgacagaatt gccgttacaa agccaaaggc
     2041 gttgtttatt attggggcaa acgacagcaa tcttgatact aagaatacag ataaggggtt
     2101 attattaaac gatgaaagag atatccttct tgaaaatgat gtaagactta caaaaggagc
     2161 agattatgac acctttaaag aaaaacatat gctttacaag atgttttcaa gtccttccca
     2221 tagtttatcc gtcagttatc ctctcggctt agaaagcgga gaaagtttgc agccatcatt
     2281 atatatcgat atattaaaga aaatctttgt ttctgtcaaa gaaagctcgg atttaagcat
     2341 ttccgatgaa ttggatttgg tgtcaaacta cagtggaacc tatgattatt ttgtagaaaa
     2401 aatgaggaat tttatagatg gttatgaaac ggataatatt tggaaggatg tttatgcatg
     2461 gtatgaaagg gaagaaaaag aaattttcga tattatggtt aagggtttaa attataaaaa
     2521 tgccatgaat tctttggagc ctgatattgt aaaaagaatt tatgaaaata atatgtctat
     2581 gacggtttct aaattagaaa gctatgctga atgtcaattt aaatacttta tggaaaatgt
     2641 gctaaaaccg agagaaagag cttatctaaa aatagaatat tatgatttag gcaatattta
     2701 tcattcaata gtggagaagt ttattaacaa aataagtgaa gataaagagg atattattat
     2761 acttgacagg acaaaagcag aggatgaagc cgtaaaaata accgatgatg ttttgttgga
     2821 gcaagctgat aagttaacag caattgatgc aaatcacaga aacaagtata tgaaggaaaa
     2881 aataaaaaga gtgatgaaaa ggacatgctg gacaattgtg cggcaattga attcgagtga
     2941 attcaggcca aaatttacag agcttcaaat tgatttggta gatgagaatg aagaaaaagc
     3001 aaaaaaggga gtttatttac ctcctataga aatacctgtg gaaacggata aaattaagga
     3061 agtgttaaaa cttaggggta aaatagacag agtagatgtt tttgaaaata acgacaaatt
     3121 atatattagt attattgatt acaaatcttc tcccaatgat atagacttag aagatgctca
     3181 agaaggaatt caagtacaat taattatgta tttaaaagca ttgcttgata aaggcgaaga
     3241 actgttcggt aaaaagcctt gtataggagg tgtgttttat tattatatca gcgaccctgt
     3301 atacaaggat aagaataaag aaaaagagcc tgaagaagaa atattcaaga gcttaaaact
     3361 taaaggatat gttttaaaag acaaagaagt tatcaagtat atggataaaa atataggcag
     3421 tacttcaagt atcataccgg cggctttaaa aagcgatgga gaaattcgcg atgaccggac
     3481 aaaagctttg acggaagagt cctttaataa tctaatcgat actgtttata acaaatgtaa
     3541 agatatgact aagagtatta tatcaggaaa tataaatata aatccttaca aaaaaattga
     3601 cggtaaaact ccgtgtaaat actgtgaata tataagtata tgccagtttg acaaaacatt
     3661 agggaataac ttccgtttaa tcggtaatca attcaaaaat ttgagcaatc tttatactga
     3721 gcactaagga ggggctaaga gatgaattgg acaaaagagc aaaagcgtat aattgattta
     3781 aaaaattcca atattttagt ttctgccgca gccggttcag ggaagactgc agtaatggtt
     3841 gaaagaatta tttcgcttat aaaaagcgga gaagatatag acaggctttt ggtagttacc
     3901 tttactaaag cggctgctgc agggatgaag caaaaaattc aaaaatcatt attgaatgct
     3961 tatcaaaaca aggatgggaa tataaagcat ctaagaaaac agttatccct cttaaacagg
     4021 tctatgatta ctacaatcga ttctttttgc ctggatgttg tgaagaaaaa cttccattta
     4081 attgatattg acccaaactt cagggttggc gacaaaagcg aattaagtat acttttgcag
     4141 gattcaatag atgaagtttt ggaagaagaa tatgaaaaaa ttaatggaaa cgaaaatttc
     4201 agaaaattag ttgaaggatt taccggaaac aggggtgatg atgagcttag tgaaataatt
     4261 cagagtacat acagatttat tctaagcttc ccagacccct ttgtatggct ttcacaaagc
     4321 gttgataagc ttaatattaa aggagaggaa ctgaaaaact caagttggct taaagaaatt
     4381 aaaagttata ttatgctttt attggacggt gcaaagggtt atatcaatac agctcttgaa
     4441 atatgcaatg agcctaaggg tccaagactt tatttggata cattaaaaaa ggatatggct
     4501 attgttgata accttgtaaa tcttcttaat aataatatgg aagagttcat ggaagccgta
     4561 aaaggattta aagcaccaaa agctccgcct tttaaagaaa aagataatcc tgacgtagat
     4621 attgaaaagc aaattgaagt gggcggttct aaaaacaata acagtatgag agccgggtat
     4681 aaaagtatta ttgcttcaat aaaggaattg ttgccttatg atttggatta tgacaagtat
     4741 tccttggaaa ttaatgaaat gtacggctca attgccgctt tgaaagattt aataataaaa
     4801 actgataaaa tttacaagga aaaaaagaat gaaaaatcca tagcagattt caatgatttg
     4861 gagcattttg cattaaatat attaagaagt aaaaatgaca agggagtatt tgagccttcg
     4921 gaagcagccg aatattacag gaaaaagtat aattatatat ttattgatga atatcaggat
     4981 tcaaacagtt tgcaggaagc aatcatcgag caaatcaaaa gagaaaataa tttgttcatg
     5041 gtaggtgatg ttaaacagag tatatacaga ttcaggttgg cagacccgag tatattcaat
     5101 aataaatata caacatacac catggacagg gaagatttaa gcaataatat tgtagacagg
     5161 actatcgatt taaacaaaaa tttcagaagc agggatgaaa ttctaaaagc tgttaatttt
     5221 attttcaaaa atattatgac aaaggaatta ggggaagtag aatataatga gaaggcagct
     5281 ttaaatacgg gaagtgagtt tttaacccat aatcctgttg aacttaatat tatttataag
     5341 aatacttctt tggaaaacca agagggagaa gctgaagccg gtgaagtgat aaaaagtgaa
     5401 gcagaaatcg aaattgaatc tatggaaaca gctgaattgg aagctttgtt tgcagcaaag
     5461 agaataaagg aattaataaa agaagaaaca tgtattccgg gaaaaacaaa gggtaaaaaa
     5521 atagaataca aggatattgt aattttatta aggtctgtag taaattgggc aggcatattt
     5581 gaagaaaggt ttaacaaaga agatattccc ttttactatg atggaggaaa gggatattat
     5641 gatgctttag aggttagaat tcttgtaaat ttacttagat taattgataa tataagacag
     5701 gatatcccgc tgttaagcgt tatgaggtca cctatcggga attttactac tgaggaatta
     5761 ttggaaataa gacttaaaca tcctaatgaa aaacatttca tagatgctct taataaatat
     5821 attctatgtg caaaagaaga agaggagtct tttcccttag ctgataaatt aaaaaatttt
     5881 atagaaaaaa tatatgattg gagttacaga agcaggtatt ctcatcttca tgatttaata
     5941 tgggatattt tgatggaatc tgattattat aattttgcgg gagctcttcc aaatggaaga
     6001 atgaggcagg caaatttgag aatgctggct gatttagcat atgattttga aaaaacatca
     6061 atgaaaggtt tgtttaaatt ccttagatat attgaaaaaa ttgataaggg ctacgatgac
     6121 aggggacagg ctaaaacatt gggagaaaac gacaatgtgg ttaggcttat gtcaattcac
     6181 aaatctaaag gattggaatt cccggtagta attctttgcg ggcttaacaa gcagtttaat
     6241 ctgcaggata caaaaaataa aatattgttg cataaggatt acggaattgc tccaatgtat
     6301 gtaaatgtca atgaaagatt tgaaagagaa acattggcaa gaactgccat taaaatgaga
     6361 ataaaaaagg aaaatatttc agaagaaatg agagttctgt atgttgcaat gacaagggct
     6421 atagataggt taataatggc agggacggtc agcaatttgg agcaaaaaga aaaaaattgg
     6481 agaagaggcc actcacaata ttttgtttat aaaggccttt cctatctgga ttggatttgc
     6541 agctgtttat ttgaaaaaat aaattttgat tattttaatg aaattatggc acagggtcag
     6601 tggaaaaact ggattttaaa tcttattaca cgggcagatt taagtttaag tataagggat
     6661 gaaatcaatt ttaaggaaag taatattagg gctatgaatg agtataaaga tatgaaaaac
     6721 agtccttatt ttgaagaaat aggaaggcgt ttgggattta aatatccata tatgtcttcc
     6781 gtaaatgtgc ctacaaagct ttccgtaacc gagcttaaaa atttgaaggg taaagaatat
     6841 gcgaaattaa gatataaaat tccaaccttg gttgatatat tcgagtacaa tgaagttaca
     6901 gataaattaa tattcaataa agaaattaca ggtgcagaaa taggaacact ccttcatttt
     6961 gtaatggagc atcttgattt aaaaggcaat ttgaaaaaat ccggcataat aagcacaata
     7021 aaacaaatgg aaaataaaaa gcttcttaca gaagctgaat caaaaatagc cattagtaca
     7081 tatgcagaaa aaatcgaaga attcttccaa agcaaaatag gaaaaagaat aattgactct
     7141 tcagcggttt acagggaggc accctttgta ttaagaaaga aagccaatga tatatcagga
     7201 agtttaaaca aagatgattt aatacttgta caaggtataa ttgactgtta tttcattgag
     7261 gatggagaag cagtaatagt tgattataaa acagataaaa tagacgaaac aaaagaaatt
     7321 gaatttcaaa tcaacaatct taaaagtgaa tataaagacc aaattgaatt atacaaggag
     7381 gcagtagaaa aaatcacaag caaaaacgta aaagaatgct atttatactt attctcaatc
     7441 ggaaaagagg ttaaaatata acattcttcg cccctgcacc tgttaatcta tgtttgacga
     7501 acctattatt ttgtgctaga cttattttat aaaaagtatt ggaggtatca atggaatatc
     7561 ttgaattgat gaaatatgtt gaccatactt tattaaaacc gtatgcaaca tgggaagaaa
     7621 ttcgtgtttt gtgtgatgaa gcaattaatt atcaaacagc ttcagtatgt attcctccca
     7681 cttatgttaa aagagtacat gatacatatc aacacaaatt aaatatttgc actgtagttg
     7741 gttttccctt aggttattct actactgaag caaaggttgc agaatgtgta caagctctta
     7801 atgacggtgc tgatgaaata gatatggtta taaatatatc tgatgtaaaa aacaaagatt
     7861 ataagaaagt agaagatgaa atccgcacaa ttaaagaaac agtcggggaa cacattttaa
     7921 aggtaattgt tgaaacatgt tatttgacca atgacgaaaa aattgaaatg tgccatgcag
     7981 tgactaaggc cagtgcggat tatatcaaga catcaacagg ctttggtaca ggtggtgcaa
     8041 ctatggagga tgtacaactt tttaaaaaat atattggaca aaatgttaga attaaagctt
     8101 ccgggggtat aagaacatta aatgatatgg aagcttttat taacgcagga tgttcaagaa
     8161 taggaacaag ttctgccatt aaattgataa ataacgctta gcgtttattt ataaaataat
     8221 aaaaaaaatg gaggaattaa tattatgaaa ttatggagaa agttggctat tgttgcactt
     8281 gtgttcataa tgatttttgc tttttcagca tgtgctaaca atcagccgga accaccggaa
     8341 gaaccggaaa caccagaaac accagaaaca ccggaaacac cggaagaacc ggaaaagccg
     8401 gcagctaatg tttttgaatt agcgttaatc actgacgttg gaacaattga tgacaaatca
     8461 ttcaaccaag gtgcctggga aggcgtagaa gcttacgcta aagaacataa tattacatac
     8521 aaatattatc aacctgctga gcaatcagac actgcttata ttgattctat tggattagca
     8581 gtaaaagcag gagcaaaggt tatcgtatgt cctggatttt tgtttgaagt gccgattcat
     8641 acagttcaaa caatgtatcc ggaagtaaac tttgtaattc ttgatggtgc accacacgca
     8701 ggcgattggg ttcctgacat tgcagacaat acatattcaa ttttctatgc tgaagaagaa
     8761 gcaggtttct tagcaggata cagcatagtt aaagaaggct ataccaagtt aggatttatg
     8821 ggcggtatgg ctgttcctgc ggttgttcgt ttcggatacg gatttgttca aggtgctgaa
     8881 tatgcagctc atgaaatggg attaacagac cctgttcaaa ttaaatacac ctatgtagga
     8941 aactttgatg ctacccctga aaatcaagca aaagcagctt cttggtacaa tgaaggaaca
     9001 gaagtaatat ttgcttgcgg cggtgcagta ggtaactctg tcatgaaggc agcagaagca
     9061 gcaggaacaa aagttatagg tgttgacgtt gaccaatcag ctgagtcaga aacagttata
     9121 acttcatcaa tgaagaacct ttcaaaatcc gtatatgatg ctttaacagc atattatgca
     9181 ggaaacttcc cgggaggtac ttcagttaca ttggatgcta ctgttgaagg tgttcaattg
     9241 ccaatggata actcaaggtt tgaaactttc acacaagctg attatgatgc aatttacgga
     9301 aaaataacag caaaagaaat agaaataatg aatgatgcag aagttgtaga agatgcagga
     9361 aaacctgcag aagaagtaac tgcggctgat attccggtaa gcaaagtaca agtagaagta
     9421 attcaatagt tatatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccat
     9541 agtagaggtg tgtccccttt ccatatcagg cgggcggggt ggtaagatgg agtacgtaat
     9601 tgaaatgaac aatattacca aaatattcgg taattttaaa gctcttgata atgtaacctt
     9661 taatctcaga aaaggtgaag tccatgcgct tttgggagaa aatggagccg gcaaatctac
     9721 tttgatgagt gttttattcg gtttatatca agcagaaaaa ggcagtataa aaattaataa
     9781 caagactgtt aaaattaata atcctaatga tgcaagcagc ttaaaaatag gcatggttca
     9841 ccagcatttc aaattagttc ataattttac ggttcttgaa agtattattt taggcagaga
     9901 aacagtaaat aagggtattt taaagaaaga tgatgcaaga aaaaaggtaa tggaactcag
     9961 cgaacgttac aagcttagaa tcgacccgga tgcatatatt tcggatataa cggttggaat
    10021 gcagcaaagg gttgaaatac ttaaaatgct gtattcagac agcgaaatat taatttttga
    10081 tgagcctaca gctgttttga cgcctcagga aattgaagaa ttaatgaaaa taatgaaaga
    10141 attggttaag gaaggaaaat caattgtttt tatatcccat aaattaaatg agataaaaga
    10201 agcagccgac cgctgcacag tcttacggaa gggcaagtat ataggaaccg tagacgtaaa
    10261 aacaacaacc aaagagcaga tgtccgaaat gatggtggga cgtaaaataa gttttaacat
    10321 agaaaaggat gatattgaaa caggcgaagc tgttcttgaa gttaaaaatt tgactgtaaa
    10381 agcaaagaat tcaaagaaaa atatggttaa taatgtttct tttaaagtta aagccggaga
    10441 aattctttgc attgcaggta ttgacggtaa cgggcaatcc gagctggtat atggaataac
    10501 aggactcata ccgattaatg aaggacaaat attctttaag ggaaaagata ttactaagta
    10561 ttcggtcaga aaaaaaagta ttaacggaat gtcgcatata cctgaagaca ggcataagca
    10621 cggagtaatt ctggattttg atttgaaaca gaatgctgtt ttacagactt acttccagcc
    10681 gcgttttcaa aaaagaggat ttatcaaatt taatgctgta agtgaatatg ccaaaaaatt
    10741 aataaaacaa tacgatataa gaagtccgag agatgaagat tcaatagtga gaaatatgtc
    10801 gggaggaaat cagcaaaagc ttatagttgc aagagagctt gataaagacc ctgatattgt
    10861 aatagctgtt cagcctacaa gaggacttga tataggagct acggaataca ttcataagca
    10921 gttaatcgct caaagagacc aagggaaggc agtgctttta gtgtctttgg aactcgatga
    10981 ggtcatggat gtaagcgaca gaattttggt aatgtatgaa ggagaaattg taggtgattt
    11041 aaatcctaaa gaaattactg tagaagaatt aggtttatac atggccggtt caaaaaggag
    11101 tgatagatat gagaagaact agactgttgc aatatgaagg ttttaccagt tttttatcct
    11161 ctttgatggc tattgtggca gggcttcttg tcggtcttat aatattgtta ataagtaatt
    11221 cagaagatgc ttttcctgcc tttttaacga ttttaaaagg aggttttgta gacggctcaa
    11281 ggggattggc tcaagttata tattttgcca tacccattat tatgaccggt ttatccgtag
    11341 gatttgcttt taaaacggga ttgttcaaca taggaacacc ggggcagttt acattaggtg
    11401 cctttgttgc tatttatata ggaattaaat ggaccttcgt tccaccggaa atacattggc
    11461 ttgttgcatt attgggggca atggttgcag gagcggtatg gggctccggt cccggtatac
    11521 tgaaagcatt tttcaatgtt aatgaagtta ttacatcaat aatgatgaat tatataggaa
    11581 tgtacctggt taatatgtca atagtgaaaa ctgtgtacga ttctttaaaa aaccaaacaa
    11641 aaccggtttt aaagagcgca gtattgccaa aggccggaat ggataagctt tttaacacta
    11701 gtaatgtcag tattggaata ttcatagtaa ttttagctgt aatagttatc tatataattc
    11761 tacagaaaac tacctttgga tacgaattaa aggcatgcgg cagcaataag gatgcgagca
    11821 aatatgcggg aatcaatgaa aagagaagta tagtgttatc aatggttatt gcaggagctc
    11881 tatcaggatt aggaggagga cttctttatc tgtcaggctc tggtaagtat atccatgtgt
    11941 tggatgtatt ggcgccggaa gggttcagcg gtatttcagt ctcgctttta ggtatgtccc
    12001 atcccatagg aatatttctt gccggattat ttatagctca tctagaagta ggcggtttta
    12061 atattcagct ctataatttt gtaccggaag taatagatat gattattgca gtaattattt
    12121 actgcggagc ttttgcatta ttgtttaaac aaataataag caaatttata attcatgata
    12181 cggatgaaga cattgggaaa tcggtgcagg cagccgatat tcctgcttct tacgaatcct
    12241 cggttgttaa tgctgaaact gaagaaaaaa cagagcaaag ggaggataag aaatgagtac
    12301 ggtttatttt ttggcacagc aaactatgta ttttgcaatt cctcttctga tagttgcttt
    12361 gggaggaatg ttttcggaaa gaagcggtat tgttaatatt gctttagaag gaataatggt
    12421 agtaggggct ttttttggaa tcgggtttat tcatatattt caagattcta tgagcggtca
    12481 aagcctattg ttattggctg tattggtatc tgccttgaca ggtgcgatat tttcactgtt
    12541 tcatgcattt gcatcgatta atttaaaagc aaatcaagtt atcagcggta cagcattgaa
    12601 tatgtttgct cctgcctttg caatttatac tgctagaatg attcaggggg ttcagcaagt
    12661 tcaattcatg gatacatttt atataaggag tgttccgatt ttgggagata taccgatatt
    12721 aggccccata atgttcaaga atgcttacat tacaacatat ataggactgt tgattgctct
    12781 aattgcatgg tttataataa atcatacgcg tttcggcctg aggcttcgag cctgcgggga
    12841 acatccgcaa gctgcagatt cagtcggtat taacgtatac catattagat atgcaggtgt
    12901 aattatatca ggtatattag ccggaatagg aggacttaca tttgtagtgc cgactagtac
    12961 aaattttaat gcaactgttg ccggatatgg attcttggca ttggcagttt tgattttcgg
    13021 tcagtggaga acgaagaaaa tattcgttgc tgcatttttc tttggaataa tgaagacatt
    13081 tgcttctgct tattcaggta taccctttct taaaggtttg ccgatatcaa atgaagttta
    13141 taaaatgatt ccttatatag ctaccttaat agtattgaca ttctcctcca agaaatctca
    13201 ggcaccaaga gcagaaggaa taccatatga taaaggaagc agataatata aaaggagata
    13261 ctatgtttgc cgataataaa atttggatag gaaaaagtga taaaaaagta tatttagagc
    13321 ctaaaatggc taacagacac ggtttggtgt caggagcaac aggcacggga aaaactatta
    13381 cattaaaagt attggcagaa tcctttagtg atttgggcgt tcctgttttt attgctgata
    13441 ttaaaggaga cttggcaagc attgcaattc cgggaacaga caatgaaaat atgcaggaga
    13501 ggctcaatcg tttagaaata aataacttta tatacaaagg ataccctgtt caattttggg
    13561 atgtatacgg tgaaggggga atacctgtac gtactacggt tacagaaatg ggtccgttga
    13621 tgctgtcaag gattttaaat ttaaatgata cacagacagg tgttttgaat atagtattca
    13681 gagtggcaga tgagagagga ctattgttgt tggatttaaa ggatctgcgt gctatggtac
    13741 aatacgtggg agacaattca aaggatttca gaatgaaata cggaaacata acggctcaaa
    13801 gtatcggtgc tattttgaga agcttgatga ccttggaaga ccagggcggt gaatttttct
    13861 tcggagagcc ggaattggat atatttgact ggataaaaac atctgcagac ggaagaggtt
    13921 atataaatgt tttgcactgt gcaaaattat ttttaaatcc cttgttgtat tcaacatttt
    13981 tgttgtggat gctgtcggaa ttgtatgaaa accttcctga agaaggggat ttggaaaagc
    14041 ctaaaattgt atttttcttt gatgaagcac atcttctttt tgaagatgct ccaaaacaac
    14101 tgcttgataa aattgaattg gtggttcggt taatacgctc caaaggtgtg ggtgtttatt
    14161 ttgtgacaca aaatccaatc gatgtgccta atgacatatt gggacaatta ggaaacagaa
    14221 ttcagcatgc tttaagagca tatacaccgg ctgaaagaaa aaaggttgtt acggctgctg
    14281 aaacattccg tgaaaatcct gaatttaaca cagtggaagc aataaccgag cttggaacag
    14341 gtgaagcatt aatttcttgc cttgatgaaa agggaatacc ttctgttgtt gagcgcgcat
    14401 ttatacttcc gccgcaaagc cggtttggaa cgatagatga tatgcttaga aaccaaataa
    14461 ttgccggtac tccgatttat aagaaatatt ataagtcagt tgacagagag tccgcttatg
    14521 aaattttatt agctaaaacg gaaagaaaat atcaggaaga acttcttgaa acagaaagac
    14581 ttgaagaaga aaaaagactc aaattagagg ctaaggaaag agaaagacaa caaaaagaat
    14641 ttgaaagagc cagaaaaaga gcaagcagca aaacaactgc cattgacagg gtagctaatt
    14701 cagccatgaa tgcaatcgga agagaagtgg gacgttcgct tataagagga gttttaggca
    14761 cctttaaaag ataatgtata ataaaaataa gggttttgaa tacgagaaaa ttgccgaaaa
    14821 atatttacaa aataataatt ataaaatatt agaagttaac tttataagca aatttggtga
    14881 aatagatatt attgccgaaa aagataatag gctgcacttc attgaagtaa aagggcgcaa
    14941 aaatattaag cacggatatc ctaaagaggc agtaacattt tccaaacaaa aaagattaag
    15001 aagtgctgct aaatattatt ttatgctgaa aggaaaagac gactatctat gtcaatttga
    15061 cgttatagaa ataatattgg aaagcagaga tataaatcat ataaaaaatg ctttttaaca
    15121 ccaagttcag attactctga acttgttttt atgacaaggg gacgnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnntaattt tactggttga tttaattaat tatctatgtt
    15301 attatcatgc aagaaattta ttgcaaggag caagaatgaa taaggataca agtaaagtat
    15361 acaccgtttc ttttataacc ttgggttgta aagtaaatca gtttgaaacg gaagctatga
    15421 ccgaaatatt agaaagagac ggatttaaag ttttgcctcc caatgaagta agcgatatat
    15481 atataataaa tacatgtgcg gttactaagg aaagcgaaag gaaatccagg caatttataa
    15541 acaaggcaaa aagaataaac cctgatgcat tagttgtggc agtgggttgc agcgtacaat
    15601 taaattccga aaaaataaaa aaagaaaccc aagcggacat aattattggg acaaaacata
    15661 aaggtaatat agggaaactt attaagaata agttaaatga tataaaacaa gaatgtaaaa
    15721 ttgatgattt tcgaggaaga gaaagcttcg aagaattaaa aataagcaat gtatacgaca
    15781 aaactcgtgt caatattaaa atacaggacg gctgcagtca attttgctca tactgcatca
    15841 ttccttacct aagaggccct ataagaagca gaaaccatga ggatataata ggggaagtta
    15901 aaagaattac aaaaaacggt tataaagaaa ttgtgctgaa cggcattcat atatcatcct
    15961 acggcaaaga gcttaaagaa aattattatc ttattgattt gattgaagat ataaataaaa
    16021 tcgaaggaat tgaaagaata aggcttggct caattgagcc taatttgata aatgaagatt
    16081 ttatgaaaag atatgcagta ttagataaag catgcgacca ttttcatttg tctctgcaga
    16141 gcggaagcga cagtgtatta aagagaatga acagaaaata ttcaaccaat gagtataaaa
    16201 acaatgtaga attaataaga aattatatgc ctgatgccgg aattacaact gatataatag
    16261 tcggattccc gggagaaaca gatgaagaat tcaatgaaac cctggagttt gtaaaaaaaa
    16321 ttaaattttc tcgaattcat gttttcaagt attccgtaag ggaaggcact aaggctgcag
    16381 aaataaaacc tcaggtaaat gaacaggtaa aagctgacag aagcaaggtt cttattgaat
    16441 tgggcaaaaa tatttcaaaa gaattcatga agaaatttat cggcaggaag ctgtcggtat
    16501 tgatagaaac caaaaaaaat gaaaatattt atgaaggtta tacaacaaat tacttgaaag
    16561 ttatgttaaa aagtgatata aatgttaaga atgaaataat taacattcat gttaccggaa
    16621 taaggaacga ttatttattg ggtgaataaa ttagtagggt gaggtgaaaa aatggattgt
    16681 atattctgta aaattgcaaa caaagaaatt ccgtctcagt ttgtatttga aaatgaatac
    16741 gtggtagctt tcagagactt agctcctcag gcacctgtac atatattagc tgtacccaag
    16801 atacacatta cttcaatgaa tgaaattaca aaggaaaaca gccatatagt tgcagaaatt
    16861 tttgaagcaa taaataaaat tacagagtca gagggcatta gggattcagg ctaccgagtt
    16921 atcagcaact gcggcagaga tggatgtcaa tcggtacaac atttgcattt tcatatttta
    16981 ggcggaaaga aattatcaga atccttagca taaattactt gcatttactt attctatgtt
    17041 atataataat aaatgctatt tcaaactcgc tggtgtccta ggacaactag tagtagaaga
    17101 cgaggggagg gtagcaagta atgacagagg taaaagtaag agaaaacgag tcaatcgata
    17161 atgctctcag aagatttaag agacaatgcg cacttacagg tgttatgtca gaagtaagaa
    17221 agagagagca ttatgaaaag ccaagcgtaa agcgtaaaaa gaaggctgaa gcggctcgca
    17281 gaaagaaaaa taaaaaagca agataagagg tgaatgtatg cctttaaaag aaaaactcat
    17341 ggaagattta aaggaatcca tgaagtctaa aaacaaagtc aaaaaagata ctgttacaat
    17401 ggttagagca gctattaaac aaaaagaggt tgacgaaaga atagaattat ccgatgctga
    17461 aattatagat ataatagcta aacaagttaa acaaaaaaaa gactcaatac ctgactttga
    17521 aaaaggcaat cgacaagatc tagtagatct aaccaatgaa gaaatcaaaa tactcttaga
    17581 atatttacca cctcagctgt cagatgaaga ccttgaatca attgtgaaag aagcaattga
    17641 aaaaacaggc gctcagtcca aaaaagatat gggcaagctc atggccttca taatgcctaa
    17701 agtaaaaggc aaagctgacg gaaagcatgt taatgaaatc gtggctaaat atataaagta
    17761 gtaagaaccc attcgggttc ttttttaatg acaaggggac gcgagggcat tctcaattaa
    17821 accctttaag ggttcttttt ttaatttttc atagtaatgt acatacctgc ataatttata
    17881 gtaacaagaa aaatgattac ttaaaagatg acggggaggg agttatgagc aagtttaaca
    17941 atttcaggag taaattagcc gatgagctgg aaataccgaa caatgcatta tcagataatt
    18001 ttgaactgag aatgcatggc aacaaaaaag ttataataga aaaccattta ggaataaagg
    18061 tttatgatga aaatatggta tgcataaaaa ccaacgacca agacataaca ataaagggct
    18121 caaaatttaa aatagaagaa atcaataatt acaaagtaat agtaaagggg aatataaaag
    18181 aaataatatt tagtatgaag gagtagtcat gttaatattc aagctgatgt atttaataag
    18241 aggttatgtg attatccgtt tggatgccaa aaactcagaa aagcttttga atatactcca
    18301 aagaagaaat attaaagtat gggatgttga aaagaaaggt aaagggataa agtttaaaat
    18361 atcatatgaa gactatttaa aaaattcaac tctaattaat gatatagtaa agcccgaaag
    18421 caaaagagga tttttgctaa agcttaacaa agtcaaatta agaaaaggct tttcaatagg
    18481 tctgttaata ttgataataa ctctttattt actgacttcc atggtatggg atgttgaagt
    18541 tataggaacc aatcagtcaa atacaaatga aatactcgcc ttattagaaa aaaatcatat
    18601 aaaaattccg gtttccattt cacgtataga agcaaaacaa ttagaaacac tgatacacaa
    18661 aaattttaaa tataaatttg ttgacgtctt tgtggaaggc tcaaaattaa ttatttttgt
    18721 aagagaaagg gagcctgacc cggctgaaat taaaagcaat gagccaagca gtatcatatc
    18781 caaaaaaaat gccattataa acaaagtaat tgctaaaagc ggtcaacctg tggttaagga
    18841 gggagacgtt gtttatgaag gccagacatt aattatggga atagtcaaaa ataaaaattc
    18901 cgatgagttt atgatggtac cttccgatgg cattatttac ggcaagacat attataattt
    18961 tgaaatgaag gaagaaaaaa taaaaagcat gaatgtgcct acaaataaaa gtaagattgt
    19021 ttattacttg aaaataaatg aaaatagtat taaaatatta ggtaataaag acccctatca
    19081 aaactatgag tatagagaga atgaaataaa agtaccgatt ttatcaaata tagctgatat
    19141 tggctttata aaaggtgtat actatgaaga agtgataaaa gaaattgaaa tagataaaaa
    19201 tacagcacaa aataaaatga aaattgctat gtatgatgaa cttttaaaaa ggtgcgacga
    19261 tgacagcagg attctaaaat caaacataaa cttttccgag gatgaaaaat tttattattt
    19321 aatagcacaa atagaggtaa ttgaagatat tggggaaaga gtaagaattt tttctacgga
    19381 ggaagaaaag acagatgaaa ccaaggagga ttaatggaat tatttgagca aaatgtctta
    19441 atagcaaatg aagatgaatt gattaaatta ttaggtattc atgataaaaa tatcaaaatt
    19501 ataaaaagtt attacgacgt taatatttta gtcagaaacg gagctttaaa gataaccgga
    19561 gaaaaggaaa attcagaagc agtctcaaaa ataataaagg acattttaaa tacgatacga
    19621 actgaaggag atataagcga ccagaaggtt atctatttaa ttgaagccaa taaatcaaac
    19681 tcgcaaattg attacaacca aattttaaaa gaagtaatcg tgacatctgc atcgggaaga
    19741 attattaaac caaagacagt cgggcagaaa agatatgtag atatgataaa ttccaaagat
    19801 gttacatttg gaatcggtcc ggcgggaaca ggcaagacat acttggctgt tgcatgtgca
    19861 gttaatgcat tcagaaaaaa agaaattgag cgtataatac ttacacgtcc tgcagtggaa
    19921 gcaggggaaa agcttggctt tttacccggg gacctgcagg ataaagtaga cccatattta
    19981 agacctcttt atgatgcgct ttttgatata atgggaattg acaactttat gaaaaatgtt
    20041 gagaagcaat tgattgaggt agctccattg gcctatatga gaggccgtac cttagattca
    20101 tcattcatta tactggatga agcacaaaac actacaaatg agcaaatgaa gatgttttta
    20161 actcgtttgg ggtatggctc aaaagccgta ataacgggag atataactca aatagacctt
    20221 cctgaaggca aaaaatcagg gcttaaatcc gtaattaata ttttaaagga tgtggaggat
    20281 ataggcttta tgtttttcca cacaaccgac gtagtcagac ataaacttgt tcaagatatt
    20341 gtaaaagcat atgaaaaata cgaggtaaaa taatgataaa agtattcttt gatgacaggc
    20401 aggatgtgat ggaaatcacc aaagacaatg aagatgctat aaaaaatgcc gtagatgcgg
    20461 tcttaacggc agaagggttg aatggagatt ttgaagtaag tgtttcattt gttaccaatg
    20521 aggaaataac ggagttaaac agggaataca ggaatgtaga tagtgaaaca gatgttttat
    20581 cttttccgat gaatgaagaa tttgacggtg ttaacattct tggagacatt gttatttcaa
    20641 ctcaaaaaat tattgaacaa gccaatgatt ttaatcacag catggaaaga gagatgtgtt
    20701 atctgaccgt acacagtatg ctgcaccttt tgggatatga ccatatgaat aaagaagaaa
    20761 agaataagat gagagcaaaa gaaaaagaaa ttatgaaaaa attaaaaata ttcaaataat
    20821 acagccgagg gggaagacgt gaaaacaaaa atagctgttt taattatatg cttgcttttt
    20881 ataaacgcat gcgcaaaaga aactgttaca cctgaacaag cggaacctga aataaatgaa
    20941 gaagaaccgg aagaaaaacc ggaagtcgat aaagaagaaa tagaggaaga aattgatcca
    21001 aataaggtac tgtcaccatt ggacgggcta agatactatc ctgaagaatt gtcaaaaaga
    21061 ccggtagccg tatctattga caaccatcac agggcaagat ggcaggcagg tattagtcaa
    21121 gccgaaatag tatatgaagt tgaagtagag catccattta ccagatactt atgtatattt
    21181 cttgcacatg agccggaaat gataggacct gtacgaagtg caagaccata tatgatatat
    21241 tatgcacttg aaaatgacgg aatatttgtg catgtgggag gaagcgaaga tgctttttca
    21301 gaaataaaaa gacttaaagt tgcagatgta gacggacttt attcaggagc tatgtggagg
    21361 tacaacgata ccggaaaata tgcccctcat aatgtgtaca caacattgga atcgattaga
    21421 aatgaagcaa aggcttacgg atacagagat gaaggaaact ttgaaggata tttgttcaat
    21481 gaaaaagatg taaaattatc ggaaaacttt gaaaccactc cgtcaaaaga agtaaacata
    21541 gtatataatt attataatac tacagattac atatttaatg aggaaaacaa tttatattta
    21601 agatttaaag acgatgaaga acatattgat gaattggata aaaagcaatt aactgcaaaa
    21661 aatattattg cagtagaaac ttccaaatca atattggata atgaaggaag attattcttg
    21721 gggaccatcg gcaaaggaaa gggcatttat atcacaaacg gagaaagtat tcctataata
    21781 tgggaaaaaa cttcagagaa agacagactg aaattttatt cgggtgatga agaacttatg
    21841 ttgaatccgg gcaacacatg gatacaggtt gttaacagcc ttgaaaatat aactttaagg
    21901 tgacaggaga agtatcgaaa taaagaaatg agaaagggga caaaatggag gataaattac
    21961 taataaaaat tgccatggaa gcaagggaag cgtcatatag tccatattcc aattataagg
    22021 taggtgctgc agtttataca aaaagcggca aggtatttac aggctgcaat atagaatcag
    22081 catcttttac tcccaccata tgcgctgaaa gagtagctgt ttcaaaatgc atttctgaag
    22141 gttataggga tatttacaag attgccgtgg ttggaagcga aataaaaaca tcttttcctt
    22201 gcggagtatg caggcagttt ttggcggaat tcggcaagga tataaaggtt atttgtgcaa
    22261 gaaacataga ttcttatgat gtttatacct tagaggagct tttgccaaac agcttcggac
    22321 ccgatgacct tgcctaagaa aggaaaatta tgtttaaatc aggatttgta actataatag
    22381 gaagaccaaa tgtgggtaaa tcaacattaa tgaataatat gttaggtgaa aaaatatcta
    22441 taatgtccga taaaccacaa acgaccagaa ataaaataag aacagtatat acggatgatg
    22501 aagcacagat aatatttata gatactccgg gaattcacaa accaaaaaat caacttggag
    22561 aatttatgaa tacggaagtg gactcagcat tagaggaaat ggacgtatta ataatgataa
    22621 ctgatgagtt taacaaagtg ggacccggag atgaatttat tattgaaaaa atcaaagaca
    22681 taaaagcaaa aaaaatactt ataattaata aaattgataa gtttgataag gaagctgcct
    22741 tgcgtatagc ggcagaattt gataagtata aggtctttga tgatatagtg cctatttctg
    22801 cccttaataa tgttggcgta gatgcattgg ttaaactgat aattaaatat ttaaaacccg
    22861 gaccgatgta ttttcctgca gattatataa ccgacaggcc ggagcgtttt attgtttctg
    22921 aaattataag agaaaaggcc ctcatgtatt tagaggatga ggtaccgcac ggaattgctg
    22981 ttgaaattga agagatgaag gaaagaaaag accgagacct tgtagatata agagccaata
    23041 ttttatgcga gaaaaagtct cacaaaagca ttattatcgg caaggaagga agaaaaataa
    23101 agggtatagg caaaagtgca agggaagaaa tcgaaaggct tttggggagc caggtaaatt
    23161 tacagctttt cgttaaaatt tccgaaaact ggcgtgatga ttcttatcgt cttaaaactc
    23221 tagggtatga taaatgatac aggaagaaat cctaattcta aaagaaataa gttataaaga
    23281 aaatgataaa atacttcatg ccttgtcaag aacaagaggt aaaattcgga ttatttcaaa
    23341 aggctcgaaa aaaagcagct cacatctgat aaatgcatcg cagatttttg catactcaac
    23401 gtgcacactc aatgtatcaa aagatatgta catattaact tccgccaaac ttttggataa
    23461 tttctacaac ttaaacaaga acatggctgc atattataac gcatcttata tattagagct
    23521 tataagttat gtagcacagg aaaatgaagt tgattccaga gtatttgata tgacggtatc
    23581 tgtgatttct catttaagca attgcacata tgaacatgaa aagcttactg ctgcctatga
    23641 attaaaattg gcggcaatgc ttgggtacaa gcctgatttt ctccattgcc ttcgttgcgg
    23701 ctcttcaata aacgatgaaa gtaagttctc cgtaacagaa ggaggggttt tttgctctaa
    23761 atgcgtaaat tttggaaatg gaataaatgt aacatataat gaaatactaa ttatggataa
    23821 aattttaaaa tctaaatttg acaatattgg ctttatagaa atacctaaga ctataatgaa
    23881 cttaataaga aaatttttgt tttattatat agggaaggat aattttacta cattaaagct
    23941 attgtaaaag aaaggatgtt catatggaaa atgatacttt aaacaaaatc gacgaaatta
    24001 taaagcgcac aaatactgat tacggtacag caaaagaagc tttggaagct gcaaacggtg
    24061 atgtgcttga agctataata ctgattgaaa atcagcaaaa aggcagcacg ggctccttta
    24121 gaggagagca gataatggct caactgaagg atatattagc taaaggaaat gctacaaaac
    24181 tcacaattaa gaaggataat gaagttatag tcaatatacc gataactgca ggcttggtaa
    24241 gtgcttttat tgcgcctttt ctttctgcag caggaataac cgtagcactg cttgcaaaat
    24301 gctctgtgga aataacacaa acagacggta aaataataga tctcgggcaa aaagttgatg
    24361 aaggaatcga tgctgtaaaa gatgctatgc atgatataaa aaaaggtgca cagaatttaa
    24421 aagatgattt aagaacaggc tcaaaaaata ttaaggatga tataaaaaca ggtgccgaaa
    24481 atgtgaagga taacataaag gccgaagctg aaaatatgaa agatgattta aaaaattctt
    24541 ttgacagaaa taatgtgcat ttttaaaaag ctcttgacat aaaaagatta ttttgttaca
    24601 ttatagtaaa ttaagactat gaaaaagaag agtattctat gcttaattta cagagagtca
    24661 gataaggtga aaactgataa ggatgtatag gagaagggag cttttgagtc tatacttttg
    24721 agaggatgaa gctttagtgc tttgtcaaat agggtggaac cgcggaagta aatctttcgt
    24781 ccctatatgg gatgagagat tttttatgtt ctcttccatt tataaggaat ggtacgcctg
    24841 cgtactttta atatatttat aacttaaaat tattatacat atggaggaat ttatggcaaa
    24901 gattgaaaaa aacagcttag aaaaagtagt atcattatgt aaatcaagag gaattatttt
    24961 tccgggctct gaaatttatg gcggcttagc taacacatgg gattacggac ctttgggagt
    25021 agagattaag aacaacataa aaagagcttg gtggaaaaaa tttatacaag aaagcccata
    25081 taatgtagga ttagatgcag caatacttat gaatcctaca acttgggttg catccggtca
    25141 cgtgggagga ttttcagacc cattgatgga ctgtaaagag tgtaaggaaa gattcagggc
    25201 tgataagctt attgaagatt ttatgaaaga aatagatgac gtacaagtgg ttgatggttg
    25261 gacaaacgat gaaatggaag catatgttgc agaaaaaaaa gtaaaatgtc ctaattgcgg
    25321 ggcacataat tttacaacca ttagaaaatt caatttaatg tttaaaactt ttcaaggtgt
    25381 aaccgaagat tctcaatccg agctttattt aagacccgaa acagctcagg gtatttttgt
    25441 taactttaaa aatgttcagc gctccatgcg aaagaaaatt cctttcggta taggacaaat
    25501 aggaaaatca ttcagaaatg aaattacacc gggtaacttt acattcagga ctagagaatt
    25561 cgagcaaatg gagttggaat ttttctgcaa acctggtgaa gacatggaat ggttctacta
    25621 ctggaaagaa ttctgtatga actggcttct aaaccttgga atgaataagg aaaaactacg
    25681 tttcagagac catgaaccgg ctgaattgtc acattacagt aaaggtacaa cagacattga
    25741 atatttgttc cactttggct ggggagaatt atggggcatt gcaaacagga ctgattttga
    25801 cctgaaacag catataaaca catccggaga ggatttatat tatcaggatc ctataaccaa
    25861 tgaaaaatac ataccatact gtgtagagcc atccttagga gcagaccgtg taatgctggc
    25921 aattctggtt gatgcatata atgaagaaac gctagaagat ggaaccgata gggttgtgtt
    25981 aaagcttcac cccgcattgt caccatataa agcggcaata ttcccgctta caaaaaaatt
    26041 aaacgagcag gctatagagc tttatcaaat gcttaacaag cattttaatg tagattatga
    26101 tgacgcggga agtataggga aaagatacag aagacatgat gaaataggaa ctccttattg
    26161 cattacattc gattttgatt cattagaaga caatacagtt accgtaaggg atagagacac
    26221 aatggagcag gttagaatta aaattgatga tttagtaaca tttattgatg aaaaaacatc
    26281 cttttaaaga tgctttcccc atttttttga gtgttttttc tgtttacaat ccagaattct
    26341 ttggaggtgt attttgaata tcagagaaaa atacgagcaa ttcgaaatag agcatctgtc
    26401 agagtatgcc tccaaggcag cactctcaaa aggaagaatg ctttatgagc cgaaatgtga
    26461 aataagaacg gattttcaga gagacagaga caggattttg cattcaaaag ctttcagaag
    26521 acttaaacat aaaacacaag tttttttatc tcctgaaggt gaccactaca gaacaaggct
    26581 tacgcacacg cttgaagtat cgcagatatc aagaacaata gcgagaagct tgaatttaaa
    26641 cgaggattta acggaagcta ttgcccttgg acatgattta ggccatacac ctttcggtca
    26701 cacgggagaa agaatactta aagagctatg caacggattt aaacacaatg agcaaagtct
    26761 aagagtcgtg gattttttgg aagtaagaaa tgaaaggcaa gggcttaatt tgacttttga
    26821 agttagagac ggtatattga atcatccttc ggggtataaa cctgctaccc tggaggggca
    26881 aatagtaagc atttcagaca gaattgcata tataaatcat gatattgatg atgcggtcag
    26941 gggacatatt attactaaaa ttccggacaa atttctaaag aatttaggct caagtcacgg
    27001 tcaaagaatt aacacaatga taatggacat aattcaaaac tcggaaaaca aaaataaaat
    27061 tgtaatgagc gatgaagttg ccgagctgac ggatgagctt cgtaattttc tgtttgaaaa
    27121 tgtatattat aataaagttg ccaaaagcga agaagataaa acaatgttca tcgttgaaaa
    27181 aatatttgaa tattacacta ataattttga acaacttcct gaattctata ttaatattta
    27241 taataataat aacttcagca agggagaaat tataaaagat tatatagcgg gtatgacaga
    27301 cagatatgca atgaaggttt ttgaagaact gtatatacct aaaccctgga tataaaaaat
    27361 aaaaaaagaa gcagaaaata ctatattaca agaatcagaa aattttaggg cttgtcaaaa
    27421 aaaatattga caagcttatt ttcctatgtt atactttatt ttcaatgtaa ggataatggt
    27481 gatattatgc cctataggtt ggattctgac gttatacaaa gaatcttgga acaaaataat
    27541 attgtagata taatagaaga atatttacct ttaaaaaaag caggtgcaaa ttacagcaca
    27601 acctgtcctt ttcataaaga aaaaacacct tcatttatag tttctccgga taagcaaatt
    27661 ttccattgct ttggctgcgg tgaaagcgga gatagtataa gttttctgac aaaatataaa
    27721 aattatacat ttgttgaagc agctgaatat ttagcaaaca gagcaggtat tatcttggaa
    27781 gaaattaact catcaagaat taacaataat aaacaaacag cagatatgct atacaaaatt
    27841 aacagagatg ctgctgctta tttttacaag aatttaagga agagtcctaa tgcaattaaa
    27901 tatttatttt ccagaaatat taaatcagat gtaataaaat cgtttggaat tggatatgct
    27961 attgacgaat gggataattt gttaaattat ctaaaaaatg aaggttataa tgaagaagat
    28021 attttaaaga ccggattaat tatttggaac gataaaaaaa atacatattt tgatagattt
    28081 agaaacagaa taattttccc tatatttgat attaaaaaaa gaattatagg ttttggggga
    28141 agagtaatag atgactctat gccgaaatat cttaattctc ctgaaagcct tatttttaat
    28201 aagggatata atttatacgg aattcatata gcaaaagaaa atataagaaa taacagcttt
    28261 attttggttg aaggatatat ggatgtaatc aagatgcatg cccatggtta taaaacagca
    28321 gttgcagcct taggcacatc ttttaccgaa aaccaagcaa aactgttaaa aagatactct
    28381 agagatttct atatagcatt tgattcggat gaagcagggc agaaagcatc tttaaaagca
    28441 attaatatgc taaaaagaaa taacttaaat gcaaaaatag ttataatgaa ggatgccaag
    28501 gacccggacg aatatctgag taaatatgga aagcccgctt tcgacaaatt aattgaaaat
    28561 tcattagatt attatagctt tttagaattt cattttaaag aaatacttga aaattctaac
    28621 aaagttgaat atataaacaa tctttttgac aatatagtaa atgtaaacag cgaaatagaa
    28681 agagaattga tttttgataa attatcaaga aaagtaggtg tttcaaaaga gtccataatt
    28741 aaagaattca ataagagaag tactaaacaa aagactttgg ataagactgc aataccggcg
    28801 gtaaaaccgc aggttgcaaa aattactaca tgccacgaag aagagctgat taaactgata
    28861 ctattagaca atgattttgc actcaagcta agtgagatag tgaatgaaga tacctttaag
    28921 gacttaaaat actataatat attaaaagag ctttatgaat gcagaattaa cgataatagt
    28981 attgacagta agagcttaaa taaaattata aaggacgaca tgaatgtaaa tttgcagttt
    29041 gacaatgttg attatgacaa tttagaggct ttgttccaag attgtatgaa aagacttaaa
    29101 ataaaatatt ataaggaaaa gagaagcata ttgaataaca ttttaaaaca atctgataat
    29161 tcattaaacc gtaaggaaat tatgaatgaa atttattgcc tcgctaaaaa gattaaatca
    29221 actaaggagg aggtcaactg acaatgagtg aaaataaaaa tatatcagta atttcagaag
    29281 aagaatatgc aaaaaacgaa gaaatgttac aaaaaataga ctccataaaa aataaattaa
    29341 ttgaaaaagg caaaagaaac ggttttataa catataaaga agtattgagg tcatttgaaa
    29401 agctagatat taatcctgaa ttcatagatg atttttataa gcaagcagaa gacaacgacc
    29461 ttgaaatatt aggtttccaa gacgatatga tacctgaaaa ggtcgatgaa gaagatgaaa
    29521 tagattcaaa agaagacgcg gatttttttg taagccaatc agatgatgat atccttaaag
    29581 gtgtaaatat agatgaccct gtaagaatgt atctgaaaga aatcgggaag gtttctttat
    29641 tgagtgcaca agaagaagta gagctggcca aaagaatgga agccggtgat gaagaagcta
    29701 agaagcagtt ggctgaagcc aatttaagat tagtagtaag cattgcaaaa agatatgtag
    29761 gaagaggaat gctttttctt gatttaattc aagaaggaaa tcttgggctt ataaaagcag
    29821 tggaaaaatt cgattataca aaaggtttta aattcagtac atatgcaaca tggtggatac
    29881 gtcaggctat tacgagggca atagcagacc aggcaagaac aataagaata cctgtgcaca
    29941 tggttgaaac aatcaataag ttaatcagaa ttaaaagaca gctattgcag gaattgggaa
    30001 gagaccctac accggaagaa atagcattgg aaatggaaat ggagcctgaa aaagttagag
    30061 aaatacttaa aattgcccag gagcccgtat ctttggaaac acccatcggt gaagaagaag
    30121 acagtcacct tggagatttc attcccgatg atgaagttca ggcaccttca gatgttgcaa
    30181 cattcaccct tttaagagag caactgtcaa atgtgttgca tactttaaca gacagagagc
    30241 aaaaggtact gaggcttcgt ttcggtttgg atgacggaag agcaagaaca cttgaagagg
    30301 tcggtaaaga gtttgatgta acaagagaaa gaatacgtca aatagaagct aaggcactga
    30361 gaaagctgag acatccaagc agaagcaaaa aattgaaaga ttatttagaa taaagatgcc
    30421 ctaaccctat ttgttcaatg tatggggaca cacctatact atagggatag tctttgtagt
    30481 tagaggaatg tccccaaatt aggttttctg aaaaatagtg gtaggtcatt cgcaacgagc
    30541 accaattttg tgcgacgaaa gtagcaaaca aaattgtgta gggagactgc gtgtaacact
    30601 ctgaatgata actatattaa tatatagatg gaatgcaaca tttacattcc atttattcat
    30661 ttaagggggt cagtatgaac agattagaat gtattgtatc aatggtcgat aaatgcaaca
    30721 cagcagcaga tataggtact gaccatggat atgtggcaga gatgctttta aaaaaaggaa
    30781 tatgcaataa aattatagcg actgatttaa acaaaggacc tttatacaga gcaagggagc
    30841 atttatccga agtaaaactc agtgataaat gtgactttag gcttggagac gggcttactg
    30901 ttttaaatga aggggaagca gatacaatta ttgttgcagg aatgggtggc gatttaattt
    30961 cagaaataat cgaaagatca aaacatatag cagagagtgc aaaacagctt attttacagc
    31021 ctatgactaa ggttgataat ttaagaagat acctttgtaa taacggtttt gaaataatcg
    31081 atgaaaatat agtaaaggaa tacaatcatt tttattttgt aataaagaca aaaaaaggct
    31141 ttaccaaata tatggacgat atatattatg aaataagtaa agtattaaca gataaaagag
    31201 ataccgtaat gatagaatac ataaataagg taattgatac aaacaaaaaa ataatcagca
    31261 gtcttgaaaa agcagataat gtggcatata atgaaaaaat agaggaatta aaaagaaaaa
    31321 acaagaaaat tagggaatta ataagatgaa tttaaataat attattaatt tattaaatga
    31381 agaagtaaag ttgaaggagc atgaatcctg ggacaacagc ggattacagc tgggctccat
    31441 ggaagttaac ataaataaaa ttatgttaac cttggacatc aataaggaag caattgaata
    31501 cgctgttcaa cataaggttg atttaataat tactcaccat ccgttttttt tcggcagtat
    31561 cagaaaaatt gattttgata catacgacgg tgaaattatt aaaatgttaa ttaaagaaaa
    31621 tataaatgtt tacagcatgc acacaagtta tgacatggct gataagggag ttacatatag
    31681 cttggcaaaa agactgaata tattaaatta tcaggttctt catccaataa atattgataa
    31741 cagcggatat gggggaatag gagatgtagc tcccgttaat ataattgagt ataccaaatt
    31801 agtaaaagaa gctcttggag ctgaatatgt caaattgtat tgcaatggag aaaaaacagt
    31861 tagaagggtt gcattttgcg gcggcagcgg cggtgaattc attgaggatg ctataaggca
    31921 aaaagctgat ctttacataa ccggagatat aaaatatcac caggcacagg aagctgtaaa
    31981 aaataattta tgcattattg atgcaggtca ttacaacacg gaaatatact caatggaaac
    32041 actaatggaa gtattagaaa aaacaggatt ggaaataatt aattttaaga aaaatataac
    32101 agctgaaaaa ataatttaga ggtggatatg gataagaatt ttgaatatat aagagtcgct
    32161 tcggcagtac catccataag agttgcagac ccaaattata atgcaattaa aattaaggaa
    32221 ataatattaa aagcggaaaa agaaaaagtc agagtcttgg tatttcctga actttgttta
    32281 acgggataca cttgtcaaga tttgtttagc cagaatacat tgataaataa atgcgaagaa
    32341 gcattaagcc ttttattgga agaaacaaag tatttgaata tgcttatagc tgtgggaatg
    32401 cccgtaaaag ccgacaccaa gctgtttaat tgcgccgtaa tatttaagga aggaaaaata
    32461 cttggtgttg ttcctaaaac atatatacct aattacaaag agttttatga aatgagatgg
    32521 tttgttcctg catcttacag gttatcagac atagttacac tttgcgagca ggaagtaatt
    32581 tttagcgaaa accttttgtt taaggatgat atatcttcgc tatgtgttgg agtggatata
    32641 tgtgaagatt tatgggcaac tattcctcca agctcatatc atgctttgca gggtgccaac
    32701 ttaatactta atttgtccgc gagcagcgaa acatcaggta aggcagatta ccgaagagat
    32761 ttggtaaaga tgcaatcttc caaatgcata acatcatatg tttatacttc ttccggcgaa
    32821 gaagaaagca caactgatgt agtattttcc ggacactgca taattgcgga agacggcaaa
    32881 atattaaagg aagataattt atgcgacggt ttcatatatt cagatattga tatagaaaaa
    32941 ataaataatg aaaaaagaag cttaaatgtt tataattcaa atatagaaag aaaaaattac
    33001 aaatatgtca attttaattt aggctacagt gaaaatttga agcttgaaag gtttgttgag
    33061 cccagacctt ttatacctgc aagacccgaa gacagagata aaagtttttc taatatccta
    33121 aaaattcagg ctgcaggtct ttctcagcgc atgaaaaaga ttggttcaaa aaaagccatt
    33181 ctggggatat caggaggtct agacagcaca ttagcattga ttgtggcggt tgaagcaatt
    33241 aagaaattaa aattaccgct tacaaatata attactgtta ttatgccggg atttggaaca
    33301 tcagagagaa cttacaaaaa tgcccatata ctgattgatg aattaggagt aaccttacga
    33361 gaaatatcca taaaggatgc atgcatgcaa cattataaag atataggaca tgatataaac
    33421 aatcatgatg ttacttatga aaattcacaa gcaagggaaa gaacacaaat tttaatggat
    33481 attgccaatc aagaaggagg actcgtggta ggaacaggtg atttatccga gcttgcctta
    33541 ggatggtgca catacaacgg ggaccatatg tcaatgtaca gcgttaattc aggagttccc
    33601 aagaccttga taaaatatat ggttaagtgg tatgcggatt ctaaaacatc aggagaaatg
    33661 caaaaaacac ttcttgatat atgcaacact cccgtaagcc ctgaactgct tccaccggac
    33721 aaggaaggaa atattcgtca gcttacggaa aactcggtag gctcatatga gctgaacgac
    33781 tttttcctgt acaatatgat gaaaaacggt tacggaccgg aaaaaatgta ctatttggca
    33841 aaaatagctt ttaaagaaga atacgacgaa caagttatcc ttgaaacatt aaaaaatttc
    33901 tataaaagat tttttactca gcaatttaag cgctcctgca tgcctgatgg ggttaaggta
    33961 ggctccgtat cattgtcgcc cagaggagat tggaaaatgc caagcgacgc atcttatgaa
    34021 ttatggatga aaatatgcga aaatatcaaa aaataaaatt cttggataaa tctgcatttg
    34081 acataaaatt ataaatatta taaaataaca aagagtaaat tatacaatcg catgcatagc
    34141 atgaggaaag tccgagctcc atagagcagg gtgccgggta acccccggtg agggtgacct
    34201 taaggatagt gcaacagaaa gaaaccgccc cgcactcaat ctgtaagaga ttcagtttat
    34261 tatttaagat atgtaaacta taaattaatt tagtatagag tcttttacag attgagtgcg
    34321 gggtaaggat ggaaaggtga ggtaagagct caccggcaat caggcgactg attggccatg
    34381 taaaccccac ccggagcaag accaaaagag gaccttggag tggctgctcg tcacggtcca
    34441 aatggtaggt cgcttgagtt tgccggcaac ggcaagccta gatagatgat tgtttaatac
    34501 agaactcggc ttatatattt actcactaca tatataatag aaaataaaat cattagcctt
    34561 tgctaagacg aaggtttttt tgttataatt tgtaacaata tcatcttcat tgaataaaat
    34621 aagtacaggc aatataaaaa gggatgatga tatgttttgt agagtagtgc catggttttt
    34681 acttggtgtt ggtttcggaa gatgcagcga gagacgtgat gtcttaggag ctaggggaag
    34741 atttgaccca tgcatcagaa gaagaagata ataaaataag ggacataact tcggtattat
    34801 ctgcagtggg cagccgcaag gtgtgtccca ttctcttata tatagattca ggctgtatat
    34861 ttagatttta ttgcgggctt tttcggctgc aataaatttt tttgcaatta atgcaattgt
    34921 taataaaatt atggaagctg caattaccca aattgcgggt attgatataa atcttattaa
    34981 aatactgtac attaatgagc cggataagcc tccgatttgt attgtaaatg attttaccga
    35041 cagcacggaa gcccttattt ggttgggagt ttcagaatta agtattacat cttccggaat
    35101 attagccatg ccgaacatta aataaattga ggcataatag gcaataaaca agggaatatt
    35161 tgtttgtaat gcggttagta tcaaaaatag agcaagtaag cttctcatta ttagatacat
    35221 ctttcttatg ttaaatttat atttgctcat aattttgttt gaaagaatac ttcctaccaa
    35281 agctgcgccc aaatataaaa atgccatcaa tcctaagaga cccgaggagc cttcatcggg
    35341 taaaagtgtt ataaagtgag gctgccaata ggtttctaat gcgcttagaa agatacctgt
    35401 tgaaaaaaca gaaatgaata taaaaagtat ggtggtgttt ttgcgaacaa aatcagaact
    35461 gttttttaca tgttgtttaa tggatttatg ttctttttta tcatgatggt ttttttccga
    35521 aataaaaaga acagatataa ttatgacagc aattgttaaa acaattctaa caataatatt
    35581 taaatcataa gttcctatgg aaggaagaag attattagaa atatccggga agaatcctcc
    35641 ggataaagca cccgctgaaa gtcccaatgc atctaagact gaaagccttg ttgtaatgtt
    35701 atgtaatttg tccttaccga aattatctat atagtagtca atgaatagcg catcaaaaga
    35761 tcctgagccg agagctcgtc ctaaaccgta aagaatcatc acaatacata atactataaa
    35821 gactttgcta atcaataata atgaaaaact taaagtaaat aatacaaggg ataatacaaa
    35881 ggtttttttt ctaccgaaga catctgccat tattccggaa ggcagttcaa gcaaaataac
    35941 cgacaaggca tataatccta atataattga taagttactc aaggaggcgc ctttatcaat
    36001 taaagccaga ctaagtaccg gggttaacag accggtcaaa tatgaattaa gaaacattat
    36061 taaagaatag atgtgcagct tcaaatttct acctccaaag gtaactattg caccgtataa
    36121 taattataat ctaaaagagt attagagtca agcgcaatta aagcatatat tgcgcgctct
    36181 gcggtcaaaa
//
LOCUS       DAOPUE010000008        34609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1178_length_34609_cov_10.210627, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000008 DAOPUE010000000
VERSION     DAOPUE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 34609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34609
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_34609_cov_10.210627"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1143)
                     /locus_tag="PKH42_01580"
     CDS             complement(151..1143)
                     /locus_tag="PKH42_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOA19147.1"
                     /translation="MFINEYEVNNITDNPYLNEIYKEAVFIDIETTGLSSIFSDIISI
                     TFLLYKDDKYRIYQIFCQYKQDQPDALKYLNELIKLKKYMVTFNGNSFDIPFLAEKAK
                     QLNITLVFDSFNKIDLYNCIKKFRYKINTVDLKLKTVEKYFSIDRNDTLDGKDVITLY
                     EAYRLEQRKEFSYLILKHNYEDVYNLPFVMNNIFSLYDFVIYTKNLIITINNEDITIK
                     KNSIKCKFNILSLIAKDYINHSINYDMVLSVKPQTLKIDIPLNIYKDNNIREFYYLDN
                     DEYNIDNYTSIKGLKRNLIPIKLNNKMYYDNINRIVKEIVEEGFGCSEKQVPSF"
     gene            1210..3525
                     /locus_tag="PKH42_01585"
     CDS             1210..3525
                     /locus_tag="PKH42_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOA19148.1"
                     /translation="MYIIISIFTAYAFGIISYELNLYGYYFTALLAILIYNSLINKKY
                     MYNAVIIAFVILSYINCNYNSSSVLSQYKNESIVVEAKVKKQKKSGSESYYASVIGIN
                     NTPILNEENIILYTGKYKNFQENSIIKARVNVAGSQLNKNKMLFNYENYLRSKKINAT
                     VFADEDAYVIEENYSFLNNISSKFRKYTEKTFYYNLNKKNAEIILSIILGDVNYLEEE
                     LYDNIKVMGLAHIFAVSGTHIVLMYIFLLNVFKFVLSRRFSWLLSWCIIWFYGFLIGF
                     PLSIMRALVMFTLLFGSEVLYRKYNSLNSIGLAALVLTIYNPFWIFDAGFLLSFSAAL
                     SFIIYGKYIKTKTTVLRTVYMYLFLQIFTLPVIVYYFNFVPVMGVLYNLLLLPVFTFI
                     MIYGFILLILNSFAHIILIIPFNIYDYILHSLRYIIDISDKSAFNGLIMPTLSLCQTI
                     FFYITLFYMIYIHNNKKCNCKKIGIFAIVSLYSITYIAFPMMDDSLYLNIADVGQGLF
                     TTIKYKGLNMICDCGSTTNKQMGEYIAVPYLTKRGIAQVDGIFISHWDEDHYSGLNDI
                     LNSHIKVNNIFSSNSNENYTNVQKLNAGCQMKYDDNLSMEILWPYKDLSLNNTNNSSL
                     VISLNYKGRRILLPGDIEEEAEYAIYNGLNAYDIVLVPHHGSNTSSTVQFVDAVRPKI
                     AVLSYGRNFYGIPHEEVISRYRNTGSKIFSTFEDGEINIVLKDNNLYYNTYMNEKSDN
                     YYKLYLIGIIYKLSIFCFLLFWMLKGESYEL"
     gene            3515..4555
                     /gene="holA"
                     /locus_tag="PKH42_01590"
     CDS             3515..4555
                     /gene="holA"
                     /locus_tag="PKH42_01590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOA19149.1"
                     /translation="MSFNLIKNMADKNKLPNCLLLYGNEEYFIDYSIKYIKYKYIDKN
                     YEDMNYAEFEKPASLKDYFEFAYTFPFMSEKKLCVIKEAGFFTSTGSLDKKEEDRLLN
                     FIDENETCISIFVIKDGKADSRKKIVKKLKDNKAVFEFNRLTEGELTKYITEEFKKNN
                     FKISMSDANYMANNTGYLEYESTVSLYHVNNEINKIMSYNKEKKNITTSDLDLLMVKS
                     IESNIFRLVDCICENDKKSSFEILNEMLLNNTPEQYIFHMITRQYRMLYQYVMLQKKG
                     YSYNEIMSKMKIKNFVASKLAKQSKNLNMETIEYYMKRFLEIDSKIKTGEIDSRVGLE
                     LISNGIIGTGTN"
     gene            complement(4579..4842)
                     /gene="rpsT"
                     /locus_tag="PKH42_01595"
     CDS             complement(4579..4842)
                     /gene="rpsT"
                     /locus_tag="PKH42_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOA19150.1"
                     /translation="MANIKSAKKRILIINKKTQANKSRKSEIKTYIKKFETALSEGNY
                     EKAKEYLKICEKKLYQAAAKNTIHKNAAARKVSRLTKKFNKVA"
     gene            5059..5991
                     /gene="gpr"
                     /locus_tag="PKH42_01600"
     CDS             5059..5991
                     /gene="gpr"
                     /locus_tag="PKH42_01600"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HOA19151.1"
                     /translation="MYRTDLAYEANESLAKKIEGITLNTHSYSHGEVVELEIKDKEAE
                     KAVGKKMGKYITYETKSLKSLSEKSRNEVIDILSKAIRDISGLGREKILVVGLGNRNI
                     TADSLGPKTLDKIKVTAQYFKAYNKDFDEDFNEVSILEPGVLGTTGIETINTIVGVVE
                     KIKPSLLIIIDALASRKMRRLCAVVQITDAGIEPGSGIGNMQGSLNKETIGTKVIALG
                     IPTVVDTATIVNDTIELMEKKLSNKTDDVGKIMGILSDLEYNEKHAFIKEILSPMYGE
                     SIVTPSYVDEIIDSLSSLLAESINRAVHPGYVNS"
     gene            6086..7234
                     /locus_tag="PKH42_01605"
     CDS             6086..7234
                     /locus_tag="PKH42_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOA19152.1"
                     /translation="MKKICGVDIMKRRRKNKDGRSFYMVMSIFCLTALFLGYSFVYNL
                     SEERKTGKEVTTIFINYNTDKSKDGNLSTFDKVMSYFNLFLEKTFKGKKSNDVELIEN
                     IDTQSYEKDIVRTIASEDTKKDLEIFNESRYEPESDSEVYLTSKRKENFFKVISKTSR
                     SSAPRRKLFINAASVINNFNIVLFHTHGTEAFEESYETNYRSLDENLNILGIGAKISN
                     NLLNKGIKNTHLKDYNDYPDYNSSYANSNYAVRQVLSDSKKNILIDLHRDGAEENSNY
                     EAILSEVKTTEINGKKAATCTLVVGNKNSNASKLKENAGRLYEIAYEMYPGLMRKIIF
                     REGAYFNQYLSDYSFLIEVGCTLNTYEEIEYTADLLTEVLYEYINEIE"
     gene            7302..9107
                     /gene="lepA"
                     /locus_tag="PKH42_01610"
     CDS             7302..9107
                     /gene="lepA"
                     /locus_tag="PKH42_01610"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOA19153.1"
                     /translation="MDRKKYIRNFSIIAHIDHGKSTLADRLIENTGLLTHRQMQEQVL
                     DNMDLERERGITIKLQTISLLYKAKDGNNYTLNLIDTPGHVDFNYEVSRSLAACEGAV
                     LVVDAAQGIEAQTLANVYLALDQDLEIVPVINKIDLPSARPDEIKQEIEDVIGLDCSD
                     APLISAKDNINIEQVLEAIVTKIPPPTGDEDAPLKALVFDSYYDSYRGVVAYIRIKEG
                     QVKKGDKIKMMSTGKTFDVTEVGIISPTQKPVPCLYSGDVGYLCASMKEVKSCQVGDT
                     ITNNANPAKEPLPGYKKVTSMVFCGIYPAEGEEYNSVRDALEKLQVNDASLIFEPETS
                     VALGFGFRCGFLGLLHMEIIQERLEREFDLNIIATTPSVIYKVYKTDGEMMVLQNPTN
                     MPPVQEIEYMEEPIVAASIMSPTEYVGAIMELCQNRRGTFVNMEYMEAKRVILHYKLP
                     LNEVIYDFFDSLKSKTRGYASLDYELDGYVRSELVKMDILINSELVDAFSIIVHKDKA
                     YERGRAIVERLRDVIPRHLFAVPLQAAIGNKIIARETVKALRKDVLAKCYGGDITRKK
                     KLLEKQKEGKKRMRQIGMVEVPQEAFLAVLKFDED"
     gene            complement(9248..9727)
                     /locus_tag="PKH42_01615"
     CDS             complement(9248..9727)
                     /locus_tag="PKH42_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19154.1"
                     /translation="MRVTIISESGDWKEMLYTKDIVTYEIKNKEILDFFVKLGDAYAN
                     NDTLDDMFECLYNLINYGYLYTDENEDDNYYDDFDYDEDYYDFDDEEQNYFSQDGFKL
                     IKTEVEYYVDNELVSKDEYDDAWDDRLGYLGSSNWEEKGLTIINADTDEIIFLGKLS"
     gene            complement(9933..10658)
                     /locus_tag="PKH42_01620"
     CDS             complement(9933..10658)
                     /locus_tag="PKH42_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA19155.1"
                     /translation="MIIRYIYHSCFLIETMNSFLLFDYFNHKNDKTDFDFSKLLKDVF
                     SSPKSLYIFSSHSHQDHFNYNVLSWINKKPSTYYILSSDIKIYNKIDNLFIVRPRDEL
                     TINEIKLNIFGSTDEGVSFMVNIDGLNLFHAGDLNWWKWYDDTPEEEKTMEDNFKSIM
                     ADIVKHGVKIDIAFFPVDRRLENNYLCGAQYFIDKLNPKLFIPMHFWDDYKVTSDFKN
                     AIAGHGTNIIEIKHPNEILYANI"
     gene            10849..12501
                     /locus_tag="PKH42_01625"
     CDS             10849..12501
                     /locus_tag="PKH42_01625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOA19156.1"
                     /translation="MKIENIVLNNNIQRALDDMGFEESTQIQQEAIPLILEGKDVVGQ
                     SNTGTGKTAAYAIPILEKIDKELRVPQALILLPTRELAVQVANEVRKIGKYMEGIKAV
                     TVYGGADIREQINKLKGGAQIIVGTPGRIIDLIERHVIKLSELRIAVLDEADEMLKMG
                     FREDIELILSKIDHPVQSLLFSATIPDDIRKIIKRFLNSPVYVKVLREGITAKEVKQS
                     YFLVKHSDKVEALSRLIDTYTPKLTLVFCNTKKAVDELYNKLIDKGYNCDKIHGDIKQ
                     SQRLDTLNKFNNGLIDILIATDVAARGLDIKEVDLVINYEVPSKEDYYVHRIGRTGRA
                     GREGASFTLVSAKELRKIEDIEKYTKKSIRKRAIPTVDRVNEVKQDKFIRDIVNVIES
                     GDLGENRELAEKLMDKGYDAETVIAAMIKKLVKFDLSEERDLNEVIPERKKRGGKLEN
                     TERFHINLGKKQGIRPGDILGAVAGECDIPGYDIGEIDVLDNFSFFAASKEHKKRILD
                     RMNRSQIKGMDVVVELSKEKKSRFGRDKSSDDNSSRKRKKRK"
     gene            12717..15239
                     /locus_tag="PKH42_01630"
     CDS             12717..15239
                     /locus_tag="PKH42_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOA19157.1"
                     /translation="MNIIVKFITKSILEKKFRSFIIIFSVTISAALFFASSALAGTMS
                     SMYEALIRMQTGKADLLIYPGRESPSSTFKILPNQVKGADYIVGTFSVGGIYKLSEEE
                     YQIERKKTELLYIKGFNIDELEKLNPINYGQKATGREFSDNHIILSEIFAKKYDYNTG
                     DSIHIEIGGVDRVLTVWGISRPTGVFQHNPQSNSIAAVMPLDTLTSLYNRRGWVDTAY
                     IVVEEGYDILNVKEEISTLYSRYVVEEPFTAEVLNEYLQFLVVPLYLMTIMVLFISVF
                     IIYSTFKVITTERLPVIGTFRSIGATRKMTDIILIGESLLYGIVGGILGVLTGMGVLY
                     LIARIMASDPYNGQLDVSIKFGLVHMLTTFILALMVATVSSWIPISRASKIPIKELVL
                     NLIDREIIKKNKKVIILTVMFAISIILSFITQNIASMIFNLLSLLIIFALVIIYIPFI
                     TSFLLKFFQQVYGAVFGNEGILAVKNLKDNKNIYNNISLLAIGISVILMINIISYSVG
                     IEVLNAYKDWKFDIMLSLNGDRNTEQVLRSIDGVEGTYGAFESFQGIKVVDTDYSIGY
                     MQGIDIIKYRDYAAFRLEGNEDVDELFKKLDDGRNIMVTLMMKDSLGLKIGDKLTLDM
                     NSGEKTYNIIGFYDSIMQNGSNAIISQKYFKTDMEQPYYNRFFVKTSKEPELVLSEIK
                     EKFMRQGVWGNSISNMEKTNYEQNSQFMIILQAFSLLAMLIGIFGVFNNYMISFLERR
                     RYIAIKRSIGLSKKQTLKMIFVEALTGGCIGATVGIIGAMFMLWIVPELMQTIGVPLA
                     IHYKSDLFAASLFTGIIISVIASVSPALKTTKLDIIESIKYE"
     gene            15249..15932
                     /locus_tag="PKH42_01635"
     CDS             15249..15932
                     /locus_tag="PKH42_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19158.1"
                     /translation="MEKIIEVENLNKTYMLGNVPVEILQEINFTINRGEFITIMGPSG
                     SGKSTLLYLLGGLDKPTSGKILINNKDISALSDEEQSTLRRRIIGFVFQFYNLVPNLT
                     VEDNVMLPVLLDGKKIKDYREKLDEILETVGLSDRINHTPRELSGGQQQRVAIARALI
                     NEPDIILADEPTGNLDSKSSEEVMKLLQRINKVKGKTILQVSHSLETAKYSDRIINVR
                     DGKVWCNEN"
     gene            15922..16944
                     /locus_tag="PKH42_01640"
     CDS             15922..16944
                     /locus_tag="PKH42_01640"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA19159.1"
                     /translation="MRIKAILMLLVIIISVAGCKAEDPQTTVEEIDLSQEKKSIEAFG
                     VVKPEEFMDIIIDFNSVVKEVSVNEGQYLKLNEPILLLDLSQYEADISDTVSELNIAE
                     LEYQQISMGFEDLSAEVNKLKNNIKYTEGLHQNTIKELDINKKLFEEGVISEEKYNQS
                     KLKAEEAENNLENLKFELSNAEDAIYKKTQSEKAKYAVQSEYIAQIKNRLNILQNKLN
                     KPYIAGNQIVSMYDNAVVYDIQYQPGHITDVSKKAFSIANLDSLTIEAEVVEEFIKDV
                     REGLKVKIVPAADRTIECEGRVIYVSDIAYSKNGGTVVPIKISLDNNDSFLKPNYNVD
                     VYIEVQ"
     gene            17304..19229
                     /gene="thrS"
                     /locus_tag="PKH42_01645"
     CDS             17304..19229
                     /gene="thrS"
                     /locus_tag="PKH42_01645"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOA19160.1"
                     /translation="MIKIKFPDKSVREYEKGIKALDVAKSISEGLARNVVGAVYNGET
                     IGLQTALNEDGNIKFLKFEDKEGKDIFWHTSSHIMAHAIKRLWPEAKLAIGPAIENGF
                     YYDIDLEHRFVQEDFEKIEAEMKKIVKAGYELERFELPREEAIKFMEEAKEDYKVELI
                     NDLPEDAVISFYKHGDFVDLCAGPHLESTKKPKAFKLLSIAGAYWRGNENNKMLQRIY
                     GISFEKAKDLEDYLKMLEEAKRRDHRKLGKELNLFMMSEEGPGFPFFLPKGVVVKNEL
                     MKYWRKLHKKAGYVEIETPLILNQHLWEVSGHWYNYRENMYITSIDEQDFAIKPMNCP
                     GGILAYKNSMHSYKDFPMRVAEAGRVHRHELSGALHGLMRVRAFTQDDAHLYILPEQI
                     KDEIKGVIKLIDEIYKQFGFSYKLELSTRPEKFLGEIKDWDLAEASLKAALDELNLDY
                     VINEGDGAFYGPKIDFHLTDCIGRTWQCGTIQLDYQLPQRFEMEYVGADGLKHRPIMI
                     HRTAFGSIERFFGILIEQYAGAFPTWLAPVQVKILPISDKFMDYAKKVERELDDLDIR
                     VELDSRAEKIGYKIREAQMEKIPYMLIVGEKEAESDTVSVRERQQGDIGSMSVDEISS
                     IILKKIQNRENDSPQLV"
     gene            19342..20109
                     /gene="udp"
                     /locus_tag="PKH42_01650"
     CDS             19342..20109
                     /gene="udp"
                     /locus_tag="PKH42_01650"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOA19161.1"
                     /translation="MGTVYSDAQYHINLKEGDVGRYVLLPGDPKRCGKIAKYFDNPVL
                     VADKREYVTYTGYLDGVKVSVTSTGIGGPSAAIAMEELANIGADTFIRVGTSGGINID
                     VTGGDLVIATGAIRMEGTSREYAPIEFPAVADYGIVNALVKAAENLNKKYHVGVVHCK
                     DSFYGQHAPESKPVSYELTNKWGAWLKMGALASEMESAALFTVAAYRKVRAGSVFLVV
                     ANQERRRLGLDDPQEHDTEGAIVTAIEALRILIKEEK"
     gene            20264..21709
                     /locus_tag="PKH42_01655"
     CDS             20264..21709
                     /locus_tag="PKH42_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA19162.1"
                     /translation="METTKKSTNYSFALDLVEDGKDIHIKEIKKNQLPPNVTSRMLYK
                     DIVKIAWPSVVELTLTQLASMVDLMMVGGLGAWALTSVGLTIQPKFLLMTTFMALNVG
                     ATAMVARYKGAGDCKRANLILRQALMITFILSLISSLLGYIFAEEFILFMGAAEEKTL
                     AGGTVYFKIQMVGFAFMALTTTITATLRGVGNSRTALTYNLIANVVNVVLNYLLIEGN
                     FGFPRMEVAGASLATIIGQLVAFVFALIYIMKGDQYLHLRFTDGFRPDSRSLKQIITI
                     GTPAMFEQLLMRAGVIIYVRAIAGLGTVAYAVHQICMSIQALSFMNGQGFAVSATALT
                     GQSLGKNRPDMAHAYVHRTRRLGMSVSIILGIIFIFWGGNIVSLYNDAPAIVSQGARI
                     MMFVGFTQPLQSSQFILAGSLRGAGDTRATAIIIFITVLIIRPFLAIYLINVWNMGLE
                     GAWFAFIVDQTVRSALVLLRYNSGKWKSIKV"
     gene            21846..22337
                     /locus_tag="PKH42_01660"
     CDS             21846..22337
                     /locus_tag="PKH42_01660"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOA19163.1"
                     /translation="MKVTLEQIDELRNRVNVSYEEAKKTLEKNDGDLIKSIIELEKKK
                     GRRNEKHHGGFTAAANKLLALRMSVKNKDGNTILNVPVVVILAAFLMAFWVVIFGLVL
                     AILTSCKITVYRDKAINVNNIKKNMQETVEKIKDKSEEIFKDDEEEPTDEQDESENEI
                     IIE"
     gene            22475..23155
                     /locus_tag="PKH42_01665"
     CDS             22475..23155
                     /locus_tag="PKH42_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA19164.1"
                     /translation="MGKKKILIVEDEQKIARFLELELKYEGYDVDIANNGREGFEKGK
                     SPDVDLIILDLMLPGLSGIEVCRRLRQTSDVPIIMLTAKDDISDKVTGLDIGADDYMT
                     KPFAVEELLARIRVLLKRKYHKTNEQDILKIGKLTLCKNNYKVEFDNENIELTKKEFE
                     LLEYLMKNKNIVISREKILDNVWGYDYYGDTNIIDVYIRYLRSKIDQKYNVSLIETVR
                     GVGYIIRD"
     gene            23166..24578
                     /locus_tag="PKH42_01670"
     CDS             23166..24578
                     /locus_tag="PKH42_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA19165.1"
                     /translation="MVKTILRKLLNLILYPFRITVKLLISLLKRLKFSIAFKISTTYL
                     ILYVIIIMTVALTTSVGYLIFELQKFEDTTAAIDSENIINSFPNFNTINVNERSYNSF
                     EIFDNNLNTYFSTGRPGRYADSIIVITEKIIFDNEYVHSESFTKNNSIYYMNINYPME
                     DMLRDAWRISFMVLISGAIGLMFFAPIVSSTSYKLIGPIKNMTEITKTITVNNINTRL
                     DVKGTQDELKELSQTFNEMMDRIESGYKTQQQFVSDASHELRTPIAVIKGYVNMLDRW
                     GKDDKAVLEESILAIKNETENMQDLIEKLLFIARSDISTLNFTKEDFRIRALLEEIEK
                     ETKMIDTAHKLYFKFYDDATIYGDKMRIKQAVRILLDNAMKYTPPGEYIMISGFIQDE
                     YYVIKVEDTGVGIKKDDLNKIFDRLYRAEQSRSKQVSGHGLGLSIAKIIVLGHKGKIK
                     VKSTPGKGSEFKLLFPYIDS"
     gene            24681..24986
                     /locus_tag="PKH42_01675"
     CDS             24681..24986
                     /locus_tag="PKH42_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451156.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOA19166.1"
                     /translation="MNIQFIETGQVLTIKLKGELDHHSADEARVLIDEKIKNEKYNKL
                     VIDLKGLDFIDSSGIGFVIGRYKVIRKRNGVIEIINAGKKVRKILDMSGIGKIIKIK"
     gene            24996..25472
                     /gene="spoIIAB"
                     /locus_tag="PKH42_01680"
     CDS             24996..25472
                     /gene="spoIIAB"
                     /locus_tag="PKH42_01680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOA19167.1"
                     /translation="MHNNYVYIKIPSLSVNESFARAAVAAFCSSLNPTMSEISDIKTA
                     VSEAVTNAVVHAYEDEIGEIEIQCRIKGQVVEIIVEDFGCGIADVEKAREPLFTTRPE
                     LERSGMGFSVMESFMDELVVLSEKDVGTKVILRKKISEKKQKTLNEEFDDEKSEDD"
     gene            25784..26224
                     /gene="spoVAC"
                     /locus_tag="PKH42_01685"
     CDS             25784..26224
                     /gene="spoVAC"
                     /locus_tag="PKH42_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOA19168.1"
                     /translation="MKYNKDNYKQVVDEFTPKNKMLKNCVMAFIFGGAICLVGEIIND
                     SFVAWLGISDEEASPMTAVVLIGISALLTGLGWYDKLGKIGGAGTVVPITGFANSVVS
                     PALEFKREGLILGTAANIFKLAGPVLVFGYFSSFIAGLLSMLFK"
     gene            26234..27226
                     /gene="spoVAD"
                     /locus_tag="PKH42_01690"
     CDS             26234..27226
                     /gene="spoVAD"
                     /locus_tag="PKH42_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOA19169.1"
                     /translation="MKKTGKTYIIKDDVVIRDTYTIVGKKEGQGPLKDKFDMVIEDDL
                     WGEDTWEKCELKMQKIAVENLKNKNSLINENIDLLISGDLLNQITSSSFAARALELPY
                     IGVYGACSTMTLTMGLGAMIIDGGFAENVICVTSSHFCSAERQYRLPLEMGGQRHMSA
                     QWTVTGAAAVWLSKSGKGPKIKHVTFGKIVDLGVKDANNMGAAMAPAAYDSLNQHLQD
                     TGIDYDLIVTGDLGTVGKGIVNKMFNEDNKSIESKYDDCGTIIFDINKQDVHAGGSGC
                     GCSAAVFGSFLYKKLLNNEIKKLLFTATGALHSPTANLQGESIPGIAHCLGIEV"
     gene            27235..27591
                     /gene="spoVAE"
                     /locus_tag="PKH42_01695"
     CDS             27235..27591
                     /gene="spoVAE"
                     /locus_tag="PKH42_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOA19170.1"
                     /translation="MNYLMSFVIGGLICVVGQIIIDTFKKNNAYLLVFFVVTGAILGF
                     FGVYDKLVEIGHSGATVPLLGFGNSLAKGAMEEAANKGFMGALSGGVIKTAPGVAAAL
                     LFGYIVAVIFNPHGDK"
     gene            complement(27794..31090)
                     /locus_tag="PKH42_01700"
     CDS             complement(27794..31090)
                     /locus_tag="PKH42_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOA19171.1"
                     /translation="MSKEKKNNEINENNSDSINKNDKKKRTAKKKRKVIIRVTLLLIL
                     VAVIIAGGAVLGMVMGIIRNAPEIDPRNVLGTLTESSVIVDENGNVIEQIHDPNENRE
                     IVPLKQIPKYLKDAFIAIEDHRFEDHPGIDIRRIMGAVIHNVREGDPTAQGASTITQQ
                     LVKNLYLTSDKVWERKITEIYLAIKMERVLSKEQILENYLNTIPLGQSQYGVQAASYA
                     YFSKDVSDLTLAESALLAAAAKSTVKYAPFNRYNLEDISDIPEEDIVGNVFIGSVQYA
                     CVYNQNAIDRQHTILNRMLELEYITQEEYDAAMAEDMRVALNPGQTKMEGISSTPMDY
                     VKEKVVEDLIATRGMSYEEAENYLYKGGLTITSTIDVNIQKSLEQSYDNFPVLYLGAE
                     PTGDKPIAQDWRYFRWSGGEGTGMLDAGLNILNESGQLIFFAKENIMDEENNIYLNPD
                     EYSIDENGNLVINSKKFDIYSSTIDIVDAYTIDDKMNFVSHTIGALNVGNNYEIIEKK
                     GSKGTFLIPKNYLDKNKEMFNIGSDGILKIPEGYFFFQEKGIVQPQSAAVIIDYKTGK
                     IKAMIGGRQIEGSKTFNRAVDAARQPGSTIKPLSVYLAALDLGYSAAYPIDDLPKYNQ
                     SGERWPKNWYEHRNIKYWGIQTLRRSIEQSINTNAVTMLETIGLDAAINSLSRLRLID
                     QNNPDKDTFVSPQEDPYYNDVNLASLALGGLTKGFSPLAMTAAYGAIANDGVYVEPIV
                     YTRVVNTKGETILENIPETHVAYSPEVASLMKDILRTTVNPGLSYRARLPQELGIEVA
                     GKTGTTQANGDFWYVGFSPYYVGGIWVGNDNVQMKLSGDSGNNARLWSAIMTPIHQGL
                     PPATFTRNPNLIPISVCKVSGKLPGDLCALDQRGSQIITEYFIPGTQPTETCDVHVKA
                     EVCTSSNMKKSIYCPGNLVEERVFFTRDPLFDPEEKTDNYEAKKLYQQILEDRIIFTM
                     EELQQIYAGQMSVDENYQITHVLGIEVEKLGPSGFITEDYQYQVPTRTCYYHTKWHYE
                     QWLNEGDGNGNNGNGNGNNGNGHNGNGNGNNGNGDTLEDIINQIIDEFENSEEDPDKP
                     IDEILESIEN"
     gene            31363..32625
                     /gene="hflX"
                     /locus_tag="PKH42_01705"
     CDS             31363..32625
                     /gene="hflX"
                     /locus_tag="PKH42_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOA19172.1"
                     /translation="MEKERCLLVAVQLPGEEENDMEELEQLVEAAGGEVISSIVQNKQ
                     SIDNRFYAGQGKIEEIANYVKELDIDTVIFNDELSGSQIRNIEEIVEAKVIDRTNLIL
                     DIFAKRALTKEGQLQVELAQLKYSLPRLIGVNKNLSRLGGGIGTRGPGEQKLEIDRRR
                     IKEKITDIQNQLEVLEKVRETKRKKRMKDQVPIVSIVGYTNAGKSTLLNALMDNEYRE
                     EGENKKVFAHDMLFATLDTELRRVRLPGGRSAVFSDTVGFIKKLPTQIVEAFKGTLEE
                     IKYADLIIHLIDINDENLSAHKETTIELIEKITGSEIPILTVYNKVDKILNEKVSVLN
                     NGIIYISAKYKYNIQELLEAVDNKINGPKNFYDLKIPNDNIKIYYWLYENRVTENVEF
                     DGDGVSLRVLLYENEKDKYKDFIGEKDE"
     gene            32618..33262
                     /locus_tag="PKH42_01710"
     CDS             32618..33262
                     /locus_tag="PKH42_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOA19173.1"
                     /translation="MSNYKSIHKEGRDEIIINRSRFIGTACPVETEEEALEFIDKIKK
                     EFKDATHNVYAYVIGENSNIQRFSDDGEPSGTAGMPVLNVIKQENIKNVAVVVTRYFG
                     GVLLGAGGLVRAYTKSSKIALESGKIVDKKLFYDVLFKLDYTLLGKMDNELLKNNVII
                     RDKEYEEQVLFKLIVEKENLDKINALVGEISSGKAEISVGQAKYYSTKNGKIVD"
     gene            33374..34114
                     /locus_tag="PKH42_01715"
     CDS             33374..34114
                     /locus_tag="PKH42_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOA19174.1"
                     /translation="MSGHSKWSNIKNRKGKQDALRGKIFTKLARAITVAAREGGADPD
                     YNSSLATAIEKAKAQNMPNDNIDRAIKAGLAASAGENFETITYEGYGPSGTAFMVQCL
                     TDNKNRTAADIRHYFSKHNGNLGTTGCVSYLFDKKGVISIESDGIDEEELMMAALEAG
                     AEDFEAQDDSYEITTIPEDFIQVLNSLKEAGYENIKGDIMYIPQTYVKVEDEAAIKNL
                     EKLIDALEENDDVQEVYHNWDEPEEEEE"
     gene            34300..>34609
                     /locus_tag="PKH42_01720"
     CDS             34300..>34609
                     /locus_tag="PKH42_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOA19175.1"
                     /translation="MLILIPAIIFTLYAQSKVKTNFAKYMRVQTRRGYTGAEVARMLL
                     NQNGLHDVPIETARGQLGDHYDPTKRVLRLSGEVYQGSSIASVSVAAHEVGHAIQHAN
                     G"
BASE COUNT        12255 a         5079 c         6716 g        10559 t
ORIGIN      
        1 gtagagcagg agactgaaaa tctccgtgtc cgtggttcga ttccgcgttt gggcatcaaa
       61 aaagaccctt aggtcttttt ttatttataa gttatgggga cacaccttct agatagctca
      121 ttgttgaagg aatgttccct tttttggttg tcagaatgac ggtacctgct tttcggaaca
      181 accaaatcct tcttctacga tttctttaac aatgcgattt atattgtcat aatacatctt
      241 attatttagt ttaatcggta tcaggtttct ttttaatccc tttatagaag tatagttgtc
      301 aatattatat tcatcattgt ctaaatagta aaactctctt atattattat ccttataaat
      361 attaagagga atatcaattt tcaatgtttg aggtttaact gacaatacca tgtcataatt
      421 tatgctatga ttaatataat cttttgctat aagagataaa atattgaatt tgcatttaat
      481 agaattcttt ttgattgtaa tatcctcatt attaattgtg ataataaggt tttttgtata
      541 tataacaaaa tcatacaaac taaaaatatt attcataaca aatggcaaat tatatacatc
      601 ctcgtaatta tgctttaata tcaaatatga aaattcctta cgctgctcta atctgtaagc
      661 ttcatacaat gtaatcacat ctttgccatc cagagtatca tttctatcaa tggagaaata
      721 cttttctacg gtttttagtt ttaaatctac ggtattgatt ttatatctaa atttttttat
      781 acaattatat aaatcaatct tattgaatga atcaaaaaca agagtaatgt ttaattgttt
      841 tgctttttct gctaggaaag ggatatcaaa gctattgccg ttgaaagtta ccatatattt
      901 cttcaatttt atcagctcgt ttaaatattt aagggcatcc ggctggtctt gcttatattg
      961 acaaaatatt tgatatattc tatatttgtc atccttataa agcaaaaaag ttatcgatat
     1021 aatatctgaa aatatactcg acaaaccggt agtttcaata tctatgaata ctgcttcctt
     1081 ataaatttca tttaaataag ggttatccgt tatattattt acttcatact cattaataaa
     1141 catagttttc ctttaataat aactttaaat ttttcctaat atttggtaaa atattagagg
     1201 gtgttgtgaa tgtatataat aatttcaata tttacagcat atgcatttgg aataatcagt
     1261 tatgagttaa atctgtacgg ctattatttt acagctcttc ttgcaatact tatttataat
     1321 tcattaataa ataaaaaata tatgtacaat gccgtaatta ttgcatttgt gatattatca
     1381 tacattaact gtaattataa ttcaagctcg gttctttctc aatacaaaaa tgaaagtatt
     1441 gtagttgaag caaaggtaaa aaagcaaaaa aagtccggca gcgaaagcta ctatgcttca
     1501 gttataggca taaacaacac tcctatttta aatgaagaaa atattattct ctatacaggt
     1561 aaatataaaa attttcagga aaacagcata ataaaagcaa gggtcaatgt tgccggcagt
     1621 caattaaaca aaaataaaat gctttttaat tatgaaaatt atttgaggag caagaaaatc
     1681 aatgcaacgg tgtttgccga cgaagatgct tatgttatcg aggaaaatta ttcgttttta
     1741 aacaatatat caagcaaatt cagaaaatat accgaaaaaa cattttatta taatttaaat
     1801 aaaaagaatg cggaaataat attgtcaata atattaggtg atgtaaacta tttggaggaa
     1861 gagctttacg ataatataaa ggtaatgggt cttgcccata tatttgctgt ttcgggaaca
     1921 catattgttc tcatgtatat tttcttatta aatgtcttta aatttgtttt aagcagaaga
     1981 ttcagctggt tattgtcttg gtgtattata tggttttacg ggtttttaat cgggtttccc
     2041 ttgtcaatta tgagggcttt ggttatgttt actctgcttt tcggctccga ggttttgtac
     2101 agaaaataca attcactgaa ttccatcggc cttgcagctc ttgtgttgac tatttacaac
     2161 cctttttgga tttttgatgc aggatttcta ctatcctttt cggcagcact aagtttcata
     2221 atatacggca aatatataaa gacaaaaacc actgttttaa gaaccgtata tatgtattta
     2281 tttcttcaaa tatttacctt gcctgtaata gtatattatt ttaattttgt ccctgtcatg
     2341 ggtgttcttt ataacttgct gcttttgccc gtatttacat ttattatgat ttatggattt
     2401 attcttttga tattaaattc atttgctcat attatattaa ttattccttt taatatatat
     2461 gattatattt tgcacagcct tagatatatt attgatattt cagataagtc tgcttttaac
     2521 ggcttaataa tgcctacttt atctttatgt caaactatat ttttctatat tacattattt
     2581 tatatgatat acatacataa taataaaaaa tgcaattgca aaaaaatcgg tatttttgca
     2641 attgtcagtc tttattccat tacctatatt gcatttccaa tgatggatga cagtctttat
     2701 ttaaatattg cagatgtggg acaaggatta tttacaacaa tcaagtataa aggattaaat
     2761 atgatttgcg actgcggcag tacaactaat aagcaaatgg gagaatacat tgctgtccct
     2821 tacttgacaa aaagaggaat agcacaagtt gatggaatat ttataagcca ttgggacgaa
     2881 gaccattatt ccggcttaaa tgacatttta aacagccata taaaggttaa taatatattc
     2941 tcatcaaaca gcaatgaaaa ttatacaaat gtgcagaaat taaatgcagg ctgtcaaatg
     3001 aagtatgatg ataatttaag tatggaaata ctgtggcctt ataaagattt atctttaaac
     3061 aatactaata attcatcctt ggtgatttct ttgaattata agggcagaag gattttgctt
     3121 cccggagata tagaagagga agcggaatac gctatttata atggtttaaa tgcttatgat
     3181 atagtcttgg ttcctcatca cggaagcaac acatcttcaa cagtgcagtt tgttgatgcc
     3241 gtaaggccga aaattgctgt tttaagctat ggaagaaatt tttacgggat tcctcatgaa
     3301 gaagttatat cgaggtacag aaatacaggc agcaagattt tttccacatt tgaggatgga
     3361 gaaataaata tagttttaaa agataacaat ttatattata atacttatat gaatgaaaaa
     3421 tccgacaatt attataaatt gtatttaatc ggaataatat acaagctgag catattttgt
     3481 tttttattat tttggatgtt gaaaggtgaa agttatgagc tttaatttga ttaaaaacat
     3541 ggcagataaa aataagcttc ccaattgcct gctgctttac gggaacgaag aatattttat
     3601 tgattattct ataaagtata taaaatacaa atatattgat aaaaactatg aggatatgaa
     3661 ttatgccgaa tttgaaaagc cggcaagctt gaaagactat tttgagtttg cctacacatt
     3721 tccttttatg tcagaaaaaa aattatgcgt aattaaggaa gccggttttt ttacatcgac
     3781 gggatcctta gataaaaaag aagaagatag actattaaat ttcattgatg agaatgaaac
     3841 ttgcatatca atttttgtaa ttaaggacgg gaaagcggat tcaaggaaga aaattgtaaa
     3901 gaagctgaag gataataagg cagtatttga gtttaacagg ctgacggaag gcgagctgac
     3961 aaaatatatt acagaagaat tcaagaaaaa caactttaaa atttcaatgt ctgatgcaaa
     4021 ttatatggca aataacaccg gttatttgga atatgaaagc acagtaagcc tgtatcatgt
     4081 caataatgaa attaataaaa ttatgtctta taataaagaa aaaaagaata taacaacttc
     4141 cgatttagat ttgttaatgg ttaaatccat tgaaagcaat atattcagat tagttgactg
     4201 catatgcgaa aatgataaga aaagttcatt tgagatattg aatgagatgc ttttaaacaa
     4261 tacccccgag caatatatat ttcatatgat tacaaggcag tacagaatgc tgtatcaata
     4321 tgtaatgcta caaaaaaaag gatatagtta caatgaaata atgagcaaaa tgaaaataaa
     4381 gaattttgta gcttccaagc ttgctaaaca atccaaaaat ttaaatatgg aaactatcga
     4441 atactatatg aagaggtttt tggaaataga cagtaaaata aaaaccggtg aaatcgacag
     4501 cagagtaggt cttgagttga tatcaaacgg gataataggt acggggacaa attaaaaaga
     4561 ccaatcaggt ctttataatt atgctacttt attaaatttc tttgttaatc tacttacttt
     4621 tcttgctgca gcgttcttat gaatagtatt ctttgcagca gcctgatata atttcttttc
     4681 gcatattttt aaatattctt tagctttttc gtagttgcct tcacttaagg ctgtttcaaa
     4741 tttcttaata tatgttttaa tttcagattt tctgctctta tttgcctgag tttttttgtt
     4801 tataattaag attcttttct ttgcagattt aatatttgcc aaaatatcca cctcctattt
     4861 cagaatttcg cagtctcatt caccaatatt atcgctaggg catctttgac agcaaatcaa
     4921 ttatacagac tattaatgca aaaatcaagt aaaaagaata aaaaatacaa aaaaactcag
     4981 aataaaaatc agatgtaaat tttatataga attttgcata tcctgagcgg cagcggagga
     5041 actatgatag gaggaattat gtacagaacg gatttggcgt atgaagctaa tgagtcactt
     5101 gcaaaaaaaa tagagggtat aacattaaat actcacagtt atagccatgg tgaagttgta
     5161 gaacttgaaa taaaagataa ggaagcagaa aaggcagtag gaaagaagat gggaaaatat
     5221 attacttatg aaactaaaag ccttaaaagt ctttcggaga aatcaagaaa tgaagttata
     5281 gatatattgt caaaagctat aagggatata tccggactcg gcagggagaa aatattggtg
     5341 gtgggattgg gaaacagaaa tattacggct gattccttag gacctaagac cctggataaa
     5401 attaaagtta cggctcagta cttcaaagca tataataagg actttgacga ggattttaac
     5461 gaggtttcta tattagagcc gggggtatta ggcacgacgg gaattgaaac gattaataca
     5521 attgttggag ttgtcgaaaa aatcaagcct tcattattaa ttatcataga tgcattagca
     5581 tcacgaaaaa tgagaagact ttgtgcagtt gttcaaataa cagatgcagg tatagagccg
     5641 ggatcaggaa ttggaaatat gcagggctca ttaaataaag aaaccatagg cacaaaggtt
     5701 atagccttag gcatacccac tgtggttgat actgccacca ttgtaaatga tacaattgaa
     5761 ttgatggaga aaaaattaag caataaaact gatgacgtag gaaagataat gggtatttta
     5821 agcgatttgg agtataatga gaaacatgca tttataaagg aaattttaag ccccatgtat
     5881 ggagaatcaa ttgtaacgcc aagttatgta gatgaaataa ttgattcatt atcaagctta
     5941 ttggcagaat ccataaacag agcagtacat cccggatatg taaattcgta atataattgt
     6001 aaaaattaac agtgttattc aggaaacaat acacatatac attaatatat tactttatag
     6061 atattttatg gagtagtata ttactatgaa gaaaatatgt ggagttgata tcatgaagag
     6121 acgaagaaaa aacaaggatg gaaggtcttt ttacatggtg atgtctatat tttgtttgac
     6181 agcacttttt ttgggataca gttttgtgta caatttatct gaggagagga agacaggtaa
     6241 agaagttacg acgattttca ttaattataa taccgataaa agtaaagacg gaaatttgag
     6301 tacatttgat aaggttatgt cttatttcaa cttatttctt gaaaaaactt ttaaagggaa
     6361 aaagagtaat gatgtagagt taatcgagaa cattgataca caatcttatg aaaaagatat
     6421 tgttagaaca atagccagcg aggatacgaa gaaagatttg gaaattttta atgaaagccg
     6481 gtatgaacct gaatctgaca gtgaagtata cctgacaagt aaacgaaaag agaatttttt
     6541 taaagttatt tcaaaaacat caagaagcag cgctccaaga agaaaattat tcataaacgc
     6601 tgcttctgtt attaataatt ttaatattgt gttgtttcac actcatggaa ccgaagcatt
     6661 tgaagaaagt tatgaaacga attaccgctc cttggatgaa aatttaaata ttttggggat
     6721 aggagcaaag atttcaaata atttgcttaa taaaggtatt aagaatactc acttaaaaga
     6781 ttacaatgat tatccggatt acaactcatc atatgcaaat tcaaattatg cggtaagaca
     6841 agttttgtca gacagcaaaa aaaatatact aattgattta caccgtgacg gtgccgaaga
     6901 gaattctaat tatgaagcaa ttctttcgga agtgaaaact accgagataa acggtaaaaa
     6961 agctgcaacc tgtaccctgg ttgtggggaa taagaattca aacgcttcga aattaaaaga
     7021 aaatgcagga agactgtatg aaatagctta tgaaatgtat ccgggactta tgcgaaaaat
     7081 catttttaga gagggtgcat attttaatca gtatttatca gattattctt tcctcataga
     7141 ggtgggatgc acattaaata catatgaaga aattgaatat accgcggatt tattaactga
     7201 agtattatat gagtatatta atgaaattga ataataatct tttacaatta cgtcaacatt
     7261 tgatataata cccatagctt aaatttggga gggtaactta tttggacaga aaaaaatata
     7321 taaggaactt ttcgataatc gcccatatag accatgggaa atctacatta gcagacaggt
     7381 tgatagaaaa tacggggctt cttacacaca ggcaaatgca ggagcaggtt ttggacaata
     7441 tggacctgga aagagaaaga ggaattacaa ttaagctcca aaccataagt ttattgtaca
     7501 aggctaagga cggaaataat tacactctta atttaataga tactccggga catgtcgatt
     7561 ttaactacga agtttcaagg agcttagcag cttgtgaagg agctgtttta gtcgtggatg
     7621 cagctcaggg cattgaggca cagacactgg caaatgtata tttggcctta gaccaggacc
     7681 ttgaaatagt tcctgttata aataaaattg atttacccag cgcaagacct gatgaaataa
     7741 aacaagaaat tgaagatgta ataggtctcg attgctctga tgcacctctt atttcagcga
     7801 aggacaatat aaatatcgaa caagttttag aagccatagt aacaaagata ccgcctccga
     7861 caggcgacga agatgcacct ttaaaagctt tggtatttga ttcctattat gacagctacc
     7921 gaggtgttgt ggcatatata agaatcaaag aaggtcaggt taaaaaggga gacaaaataa
     7981 aaatgatgtc aacaggtaaa acctttgatg tcacagaggt tggaataatt tcacctacac
     8041 agaagccggt accttgtctt tattcgggag atgtgggtta tttgtgtgcc agcatgaagg
     8101 aagtaaaaag ctgtcaggtt ggagatacaa taactaataa tgcgaatcct gcaaaagagc
     8161 ctttgccggg atataaaaag gttacgtcca tggttttctg cggtatttat ccggcagagg
     8221 gagaggaata caacagtgtg agagatgcat tggaaaaact tcaggtaaat gatgcttcct
     8281 taatttttga gccggaaaca tcagttgcct taggatttgg attcagatgc ggatttttgg
     8341 gtcttttgca tatggaaata attcaggaaa ggcttgaaag ggaatttgat ttaaatatta
     8401 tagcaacaac tccaagcgta atatacaaag tatataagac tgacggagaa atgatggttt
     8461 tgcaaaatcc tacaaatatg cctcccgtcc aggaaatcga atatatggaa gaaccgatag
     8521 ttgctgcatc cataatgagt cctaccgagt atgtgggagc aattatggag ctatgccaaa
     8581 accgcagagg tacatttgta aatatggaat atatggaagc gaaaagagtt attctacatt
     8641 ataaattacc tttgaatgaa gtaatctatg atttctttga ttccttaaaa tcaaaaacaa
     8701 gaggatatgc atcattggat tacgagcttg acggttatgt acgctccgaa ttggttaaaa
     8761 tggatattct aataaattcc gaattggtag atgccttttc aataattgtt cataaagata
     8821 aagcttatga aagaggaaga gcaatagttg aaagacttag agatgtaatc ccgaggcatt
     8881 tgtttgcagt gcctcttcaa gctgcaatag gaaataaaat aattgcaaga gagaccgtaa
     8941 aagcactcag aaaagatgtt cttgcaaagt gttacggcgg agatataacc agaaagaaaa
     9001 agcttttgga aaagcaaaaa gaaggcaaga aacgaatgag acaaatagga atggtggagg
     9061 ttccacagga agcattcctt gcagttttga aatttgatga agattaagta tgccctaacc
     9121 ccatttatca gagtattttt tctgataaat ggttggtagg tcaaccgcac agaatgccaa
     9181 atttgtgcga cgaaaggagc aaacaaattt gatgcattcc cctgcgaggg ctttgtgccc
     9241 ttttttatta tgacaactta cctaaaaaaa tgatttcatc ggtatcagca ttaataatag
     9301 taagaccctt ttcttcccaa tttgatgagc cgagataacc caacctgtca tcccatgcat
     9361 cgtcgtattc gtcttttgat accaattcat tatcaacata gtattcaact tctgttttaa
     9421 ttaatttaaa accatcttgg ctaaaatagt tttgttcttc atcatcaaaa tcataataat
     9481 cttcatcata atcaaaatca tcataataat tatcatcttc attttcatca gtatatagat
     9541 aaccgtaatt tatcaaattg tacaaacatt caaacatatc atcaagtgta tcattgtttg
     9601 catatgcatc gcccaatttt acaaaaaaat ccaatatttc cttattttta atttcatatg
     9661 ttactatatc cttggtgtaa agcatttctt tccaatctcc gctttcactg attattgtta
     9721 ctctcatatt actccccctt gccaccataa ctgttagtaa aattttatgc gcccaaatac
     9781 atattatgtt tatgattagt gaaaacagag aataaatatt ttgtttgtta tttgaattga
     9841 gtcaaaaaaa aaggtcattc tatgaataac tttcattaga aggcaacgac aagaacgggc
     9901 tatccataga aatgacaatg aagaagataa tactatatat ttgcatacaa tatttcattg
     9961 gggtgcttga tttctattat atttgtgcca tgcccggcaa ttgcattttt aaagtctgat
    10021 gtaactttgt aatcatccca aaaatgcatt ggaataaaca gcttagggtt taatttatca
    10081 ataaagtatt gagcgccgca caagtaatta ttttcaagcc ttctgtcaac aggaaagaat
    10141 gcaatatcaa ttttcacgcc gtgctttaca atatctgcca taatgctttt gaaattatct
    10201 tccatagttt tttcttcttc aggcgtatca tcataccatt tccaccagtt taaatcaccg
    10261 gcatgaaata agtttaaacc gtctatattg accataaaac taactccttc atcagtagaa
    10321 ccaaaaatat tcagttttat ttcattgatt gtaagttcgt ctcttggtct tactataaat
    10381 agattatcaa ttttattata aatttttata tcagaggata atatgtaata tgtactcggt
    10441 tttttattta tccaggataa aacattataa ttaaaatggt cttgatgtga atgagagctg
    10501 aatatataaa gagatttagg gcttgagaat acatctttga gcaatttaga aaaatcaaaa
    10561 tcagttttat catttttgtg attgaaataa tcaaaaagca aaaatgaatt cattgtttct
    10621 atcagaaaac agctatggta aatatatcga attatcataa tacctccaca aatatatatg
    10681 agtttcttaa ttataatata atcctaaatt atgagcaata ctataattaa atataactta
    10741 agacagtatg ctcataaatt tttgtaatta caaaataaat gtttgtaatt attaaatccg
    10801 cattatataa tgagaagttg aaaaaaatgg aaagaggtaa aaaaatcaat gaaaattgaa
    10861 aatatagttt taaataataa cattcaaaga gctttggatg atatgggctt tgaagaaagc
    10921 acccaaatcc agcaagaggc aattccttta atattggaag ggaaagatgt tgtaggacag
    10981 tcaaatacgg gaaccggaaa aacagcagct tatgcaattc ctattcttga aaaaatagac
    11041 aaagaattga gagtgcccca ggcattaatc cttctgccaa cgagagagct ggctgtccag
    11101 gttgccaatg aagtaagaaa aataggcaaa tatatggaag gtatcaaagc tgtaacagtg
    11161 tatggaggag ctgatatcag agaacagata aataaactta agggaggagc tcaaataatt
    11221 gttggaaccc ccggcaggat tatagatttg attgaaagac atgtaataaa acttagcgag
    11281 ctaagaattg ctgttttgga cgaagctgat gaaatgctta aaatgggatt tagagaggat
    11341 atagagctta ttttaagcaa aattgaccat cccgttcaat ccttattgtt ttccgcaaca
    11401 attcccgatg atatcagaaa aataattaag agatttctta attcccctgt ttacgtgaag
    11461 gttttaagag aaggaataac agccaaggaa gttaaacaaa gttatttttt ggtgaagcat
    11521 tccgataagg ttgaagcact ttccaggtta attgacacct atacaccaaa acttacattg
    11581 gtattttgca atacaaagaa ggcagtggat gagttatata acaagcttat cgataaaggg
    11641 tataactgcg ataaaattca cggagatata aaacaatcac aaagattaga tactttaaac
    11701 aagtttaaca atgggcttat tgatatactt atagctacag acgtggctgc aagaggcctt
    11761 gacataaaag aagtagatct tgtaataaac tacgaagttc cttcaaaaga agattattat
    11821 gttcaccgta taggcaggac ggggagggcc ggaagagaag gagcatcatt taccttggta
    11881 agtgcaaaag aattaagaaa aatagaggat attgaaaaat ataccaaaaa atcaatccga
    11941 aaaagagcaa tacccacagt ggacagggta aatgaagtta aacaggataa atttataaga
    12001 gatatagtaa atgtaataga aagcggagat ttaggggaaa acagagaact ggcagaaaaa
    12061 cttatggata agggctatga tgccgagact gtaattgcag ctatgattaa aaagcttgtt
    12121 aaatttgatt tatcggaaga gagagattta aatgaagtta ttcccgaaag aaaaaagaga
    12181 ggcggaaagc ttgaaaatac agaaagattc catattaatc tcgggaaaaa gcaagggatt
    12241 cgaccgggtg acatattagg agcagtagca ggagagtgcg atattcccgg ctatgatatc
    12301 ggagaaatag atgtactgga taatttttca ttctttgctg cttcaaagga gcataaaaaa
    12361 agaattcttg atagaatgaa ccgctcgcaa ataaaaggaa tggatgttgt tgtagaatta
    12421 tctaaagaaa aaaaatcacg atttggaaga gataaatcat cagatgacaa ctcatcccga
    12481 aaaagaaaga aacgaaaatg attaactaac ccatctttat gcagtaataa aaatgtgact
    12541 tatatgatga cctaagtcac tttttatttt atttttcttc tcatataatg aattcataat
    12601 aaaagcgctt ttaagagcaa caaaaaaata aaaaaattct tgtaataaat atgcatatat
    12661 ggtataacaa ttaatggaaa atattaaagc taatgtattt ttttggcggt gttttaatga
    12721 atattattgt taaatttata acaaaaagta ttttagaaaa aaagttcagg tcttttataa
    12781 ttattttctc agttacaatt tcagcagcct tattttttgc ttcaagtgct ttggcaggta
    12841 ccatgagttc catgtatgaa gctcttatta gaatgcagac cggcaaagca gatttattga
    12901 tttatcccgg cagggaatca ccttcttcca cttttaaaat attgcccaat caagtaaagg
    12961 gtgctgatta tattgtagga actttttcgg ttggcggaat ttataaattg tcagaggaag
    13021 aatatcaaat tgaaagaaaa aagacagagc tgctatatat aaaagggttt aatatagatg
    13081 aattggaaaa acttaatccc ataaattatg gtcaaaaggc aacaggcagg gagttttcag
    13141 acaatcatat tattttaagc gaaatatttg caaaaaaata tgattataat acgggagaca
    13201 gtatacacat agaaatcggc ggtgttgaca gagttttaac cgtatggggt atttcaagac
    13261 ctacaggtgt atttcagcat aatccgcagt caaattctat agccgcagta atgcctctag
    13321 atactcttac ttctttatat aatcggagag gttgggttga tactgcttat attgttgttg
    13381 aagaaggata tgatatttta aatgtaaaag aagaaataag caccttgtat agcagatatg
    13441 ttgtagagga gccttttaca gcggaagttt tgaatgagta tctgcaattt ttagttgtac
    13501 ctttgtattt aatgaccatt atggtgttat ttatcagtgt ttttattatt tattccacat
    13561 ttaaggtaat aacaacagaa aggcttcctg tcataggtac ttttcgaagc attggtgcta
    13621 caagaaaaat gacggatatt atactcatag gcgaaagctt attgtatgga attgtggggg
    13681 gaatattagg agtattaaca ggaatgggag ttttatatct tattgcccga ataatggctt
    13741 cagaccctta taacggacag ttggatgtat cgattaaatt tggcttagtc catatgctga
    13801 cgacctttat tttagccctt atggttgcaa ctgtcagttc ttggattcca ataagtaggg
    13861 cttcaaaaat accaataaaa gaattggtat tgaatttgat tgatagagaa ataatcaaaa
    13921 aaaacaaaaa ggtcattata ttgactgtta tgtttgcgat atcgataatt ttatctttca
    13981 taacacaaaa tatagcatca atgatattca atcttttgtc tttattgata atatttgctc
    14041 ttgtaataat ctatattcct tttattacat catttttatt aaagtttttt cagcaagtct
    14101 atggagctgt atttggaaat gaaggaattc ttgcggttaa aaatttaaag gataacaaaa
    14161 atatatataa taatatatca ttgctggcaa ttggaatttc ggttattctc atgattaata
    14221 taatcagtta cagcgtaggt atagaagtgt taaatgctta taaggactgg aagtttgata
    14281 taatgctttc cttaaacggc gatagaaata cggaacaggt acttagaagt attgacggag
    14341 tggaaggaac ttacggagcc tttgaaagtt ttcaaggaat aaaagttgtg gataccgatt
    14401 acagcattgg atatatgcag ggtattgata tcatcaagta cagggattat gcagcttttc
    14461 gtctggaggg caatgaagat gttgacgagc tgttcaaaaa gcttgatgac ggcagaaata
    14521 taatggttac tcttatgatg aaagacagcc tgggattaaa aataggcgac aaacttacat
    14581 tagatatgaa ttccggcgaa aaaacctata atataatcgg tttttacgat tcaataatgc
    14641 aaaacgggag taatgcaatt atatctcaga aatattttaa aacagatatg gagcagccat
    14701 attataacag attttttgta aaaacatcta aggagccgga gttagtccta tcagaaatca
    14761 aggaaaagtt tatgagacag ggcgtatggg gtaattcaat ttcaaacatg gaaaaaacaa
    14821 attatgagca aaatagtcaa ttcatgatta tccttcaggc attttctctt ttagctatgc
    14881 tcataggtat cttcggggtc tttaacaatt atatgattag ttttttagag cgcagacgtt
    14941 atattgcaat taagcgttcc ataggactga gcaaaaagca aacattgaaa atgatatttg
    15001 tggaggcttt gacaggcgga tgtattggtg caacagttgg aattataggt gcaatgttca
    15061 tgctttggat agtacctgaa ttgatgcaga caataggtgt tcccttagcc atacattata
    15121 agtcagattt atttgcagcc tcattattta caggaattat aatctctgtt attgcttctg
    15181 taagtcctgc attaaaaaca actaagttag acattatcga atcaattaaa tatgaatagg
    15241 ggatagttat ggagaaaatt attgaggttg aaaatttaaa taaaacgtat atgttaggaa
    15301 atgtacccgt agaaattttg caggaaataa actttacaat aaacagggga gagtttataa
    15361 caataatggg tccttcaggt tcaggtaaaa gcacattgct atacttgttg ggaggattgg
    15421 ataaacctac atcagggaaa atacttataa ataataagga tatttccgca ttgagtgatg
    15481 aggagcaaag tacattaaga cgaagaataa tcggattcgt atttcaattt tacaatttag
    15541 ttcctaacct gactgtagaa gataatgtta tgctcccggt attattggac gggaagaaga
    15601 ttaaggatta ccgggaaaaa ttggatgaaa tacttgaaac agtaggattg tcagacagaa
    15661 taaatcatac tcctagagag ttatccggag gacagcagca gcgtgttgca atagctcgtg
    15721 cattaattaa tgagccggat attatattag ccgatgagcc tactggaaat ttagacagta
    15781 aatcatcgga agaagttatg aaactgttac aaagaataaa taaagtgaaa ggaaaaacca
    15841 tattacaggt aagtcattcg ctggaaacgg ctaaatatag tgatagaatc attaatgtaa
    15901 gggatggaaa ggtgtggtgc aatgagaatt aaagcaatat tgatgcttct tgttataata
    15961 ataagtgttg ccggctgcaa agctgaagac ccgcagacaa ctgttgagga aatagattta
    16021 tcccaagaaa aaaaatcaat tgaagccttt ggagtagtta aaccggagga attcatggat
    16081 attataattg attttaattc cgtggttaag gaagtttctg taaatgaagg acagtatctg
    16141 aaattgaacg aaccaatact cttattggat ttgtcacaat atgaagcaga tatatcagac
    16201 actgtaagcg aattaaatat tgctgaattg gaatatcaac aaatcagtat gggatttgaa
    16261 gatttaagcg ctgaagttaa caagcttaaa aataatataa aatataccga aggcttgcat
    16321 caaaatacaa taaaagaact tgacataaat aaaaagctct ttgaagaagg agtaatttct
    16381 gaggaaaaat ataatcaatc aaagttaaaa gctgaagaag cagaaaataa tttagagaac
    16441 ctcaagtttg agttgagcaa tgcagaagat gcaatttata aaaaaactca atcagaaaag
    16501 gcaaaatatg ctgttcaatc ggaatatata gctcaaatta aaaacagatt aaatatttta
    16561 caaaataaat taaataaacc gtatattgca ggaaatcaaa ttgtttccat gtatgataat
    16621 gcagtagtat acgatataca atatcaaccg ggacatataa ccgatgtttc aaaaaaggct
    16681 ttttcaatag caaatcttga cagcttaaca attgaggcgg aagttgttga agagtttatt
    16741 aaagatgtac gggaaggctt aaaggtaaaa atagttccgg ctgcggacag gactatagaa
    16801 tgtgagggtc gtgttatcta tgtttctgat attgcttaca gcaaaaatgg cgggacagta
    16861 gtgccaatta aaatatcatt agataataat gattcatttc tgaagcctaa ttacaatgta
    16921 gatgtctata ttgaagtaca gtgaggatat tttaaagatt aaaaaatata aatttactgt
    16981 tgacaaaaaa atttaccggt aatataatgg tcaaaatgaa ctgtgatgga tcgagtagaa
    17041 cagacacagc ttaacagaga gggaatgttg gtggaagttt cccatgtatg actgtttgaa
    17101 aactaatccg gagttaagac agaaagtcaa atggcgtaat gtcttggtat ggatgcctta
    17161 aatcctttga gaagcaacta aggtggaacc acggtatctc gtccttaaac tatgtttagg
    17221 aacgagattt tattttatta acgactgtca tcctgacggg ttattaatag caattacact
    17281 aatttctaag aggaggaata gttatgatta agattaaatt tcctgacaaa agcgtcagag
    17341 aatatgaaaa aggaattaaa gctttggatg ttgccaaaag tatcagtgaa ggattggcaa
    17401 ggaatgtggt gggagccgta tataatggag aaacaatcgg acttcaaacc gcattaaacg
    17461 aagatggaaa tattaaattt ctgaaatttg aggacaagga aggaaaagat attttctggc
    17521 atacttcatc tcatattatg gctcatgcca ttaaaagact gtggccggag gcaaagcttg
    17581 ccataggacc tgcaattgaa aacgggtttt attatgatat tgatttagaa caccgatttg
    17641 tgcaggaaga ctttgaaaaa atcgaagctg aaatgaagaa aattgtaaaa gccggctatg
    17701 aattagaacg ttttgaactt cccagagaag aagcaattaa gtttatggaa gaagcaaaag
    17761 aagattacaa agtcgagtta attaacgact tgcctgaaga tgcggtaata tcattttaca
    17821 agcatggtga ttttgttgat ttgtgtgcag gacctcatct tgaaagcaca aaaaaaccaa
    17881 aggcttttaa acttctcagc attgcaggag catactggag aggaaatgaa aacaataaga
    17941 tgctccagcg tatatacggt atttcatttg aaaaggcaaa agacttggaa gattatttga
    18001 aaatgttgga agaggcaaaa agacgtgacc acagaaaact tggaaaagaa ttgaatttat
    18061 tcatgatgtc cgaagaaggt cccggatttc ctttcttcct accaaaaggc gttgtagtaa
    18121 aaaatgaact tatgaaatat tggagaaaac ttcataaaaa agcaggttat gttgaaatag
    18181 aaacaccttt aattctaaat cagcatttat gggaagtatc cggacattgg tacaattata
    18241 gagaaaacat gtatattact tcaatagatg agcaagactt tgctattaag cctatgaact
    18301 gcccgggtgg aatcttagca tataagaatt ccatgcactc atataaagat ttccccatga
    18361 gagttgcaga agcaggaaga gttcacagac atgaattatc gggtgctttg cacggcttga
    18421 tgcgtgtaag agcatttaca caggatgatg ctcatttata tatacttcct gagcaaatta
    18481 aggatgaaat taaaggcgta ataaagttaa tcgatgaaat ttataagcaa tttggtttct
    18541 catataaatt ggagctttca acaagaccgg aaaagttttt gggagaaatc aaggactggg
    18601 atttagcgga agcatctcta aaagcagcat tggatgagtt gaatttagat tatgttataa
    18661 atgaaggtga tggagctttc tacggaccaa aaatcgactt ccacttaaca gactgcatag
    18721 gaagaacctg gcagtgcgga acaattcaac tggattacca gcttcctcag cgctttgaaa
    18781 tggaatatgt gggagcagac gggcttaagc acagaccgat aatgattcac agaaccgcat
    18841 ttggaagtat tgaaagattc ttcggaattc ttattgagca atatgcagga gcattcccga
    18901 catggcttgc accggtgcag gtaaaaatac tgcctatttc ggataagttt atggactatg
    18961 caaaaaaggt tgaaagagaa cttgatgatc ttgatataag ggttgaatta gactcaaggg
    19021 cagaaaagat aggatataaa atcagagaag ctcagatgga aaaaattccg tacatgttga
    19081 tagtagggga aaaagaagct gaaagcgaca ccgtttccgt aagagaaaga caacaaggag
    19141 atattggaag catgagtgtt gatgaaataa gcagcattat tttaaaaaag attcagaata
    19201 gggaaaatga ctctccacaa ctggtttgat tgaaaaatga ggcatgcaat ttgcagcctc
    19261 ttttttataa taaaaaagat attgaatttt accaatattt tatatattat gtagataatt
    19321 aaaattatgg aggtatgcag catgggaaca gtatactcgg atgctcaata tcatataaat
    19381 ttgaaagaag gagacgtggg caggtatgta ttattgcccg gagatcccaa aagatgcggt
    19441 aaaattgcta aatactttga taatcccgtg ttagtagctg acaaaagaga atatgttaca
    19501 tatacaggct accttgatgg agtgaaggta agtgtgacat caacaggaat cggaggacct
    19561 tctgctgcaa ttgcaatgga agaactggca aacataggag cggatacatt tataagagtt
    19621 ggtaccagtg gaggcataaa tattgatgtt accggcggtg acttggtaat agccacagga
    19681 gcaatccgta tggaaggtac aagcagagaa tatgccccca tagaatttcc ggcagttgct
    19741 gattatggaa ttgttaatgc attggtaaaa gcagcagaaa atttaaataa aaaatatcat
    19801 gtaggtgttg tacattgcaa ggattctttt tacggacagc atgcaccgga gtcaaaacct
    19861 gtaagctatg agttgacaaa taaatgggga gcatggctga aaatgggtgc cttggcatct
    19921 gagatggaat cagcggcatt gtttacggtt gcagcttaca gaaaggttag ggcaggctct
    19981 gtatttcttg tagtggcaaa ccaagaaaga agacgtctgg gtcttgatga cccgcaggag
    20041 cacgatactg agggagctat tgttactgca attgaagcgc tgcgaatttt aataaaagag
    20101 gaaaaataat accggattta ttgaatttta ttggtagctt tagcagccgg ttttgttgac
    20161 attgcaaaca aatcatgata tcataaatta taacgaattt tagcggctca aaccgcaaaa
    20221 aagtcgcatc tatgcgactt tatttatgcg acaaggagca acaatggaaa caacaaaaaa
    20281 atctactaat tatagctttg ccttggactt ggtagaagac ggcaaggata ttcatataaa
    20341 agaaattaaa aaaaatcagc ttcccccgaa tgtgacttcc agaatgcttt ataaagacat
    20401 tgtaaagata gcatggccat ctgttgttga gcttacctta actcagcttg catcaatggt
    20461 agacttgatg atggttggag gtttgggagc ctgggcactt acttccgtcg gtcttacaat
    20521 tcagcctaaa tttcttctca tgacaacttt catggccttg aatgtcggcg caactgcaat
    20581 ggtggcaaga tacaaaggag caggagattg taaaagagca aacttaattc ttaggcaagc
    20641 cctgatgatt acttttatac tttctttgat ttcttctttg ttaggttata tttttgcgga
    20701 agaatttatc cttttcatgg gtgcagccga agaaaaaaca ttggcaggcg gtacagtata
    20761 ttttaaaatt caaatggttg gctttgcatt catggcattg accacaacaa tcacggcaac
    20821 attaagaggt gtcggaaact caagaacagc attgacatat aatcttatag ctaatgttgt
    20881 aaatgtagtt ttaaattacc ttttaattga agggaatttt ggttttccgc gcatggaagt
    20941 tgcgggtgct tctttagcaa ctattatcgg acaacttgtt gcatttgttt ttgcattaat
    21001 ttatattatg aagggcgacc agtatttgca ccttcgcttt acagatgggt ttaggcctga
    21061 ttctcgttcc ctcaagcaaa taataacaat aggtactccg gcaatgtttg aacagctgtt
    21121 gatgcgtgcc ggagtcataa tatacgtaag agcaattgcg ggtcttggta ctgttgcata
    21181 tgcagtccat caaatatgta tgagtattca ggcattatcc tttatgaacg gtcagggttt
    21241 tgccgtgtca gctactgcac ttacgggaca aagcctgggt aaaaaccgcc ccgatatggc
    21301 acatgcctat gttcacagaa caagaagact tggaatgtct gtttcaatta tattgggaat
    21361 aatctttata ttctggggcg gtaatatagt atccttatac aatgatgccc cggcaatagt
    21421 ttcccaaggt gcaagaataa tgatgttcgt aggctttaca cagcccttgc agtcttccca
    21481 gtttattttg gcaggctcat taaggggtgc cggagataca agagcaactg caattattat
    21541 ttttataacg gtactgataa taagaccttt tttggcaata tatttaataa atgtatggaa
    21601 tatgggactg gaaggggctt ggtttgcttt tatagttgat caaactgtac gttcggcact
    21661 tgttctttta aggtataatt caggaaagtg gaaaagtata aaagtctgaa aattcatact
    21721 tttcatattt cttaaaatta tttttcatat tttaatctaa ttttaattta tacctaaaac
    21781 ttaattaata ctttggtggt accttatata taagaaaaaa ataaaattta tatataggag
    21841 gaactatgaa agttacattg gaacaaatcg atgaactcag aaacagagtt aatgtaagct
    21901 acgaggaagc taaaaaaacc ctcgaaaaaa atgacggaga tttaattaaa tcgatcatag
    21961 agcttgaaaa gaaaaaagga agaagaaacg aaaaacatca tggaggcttt acagcagccg
    22021 caaacaagct tttggctctt aggatgtctg ttaaaaacaa ggacggaaat acaattttaa
    22081 atgtgcccgt ggtagtaata ttagctgcat ttttgatggc attttgggtt gtaatcttcg
    22141 gattggtact ggcaatattg acatcctgca aaataactgt ttacagggat aaagcaataa
    22201 atgtaaacaa tataaagaaa aatatgcagg aaacggtgga aaaaataaaa gacaagtcag
    22261 aagaaatctt taaggatgac gaagaagagc caacagacga acaagatgaa tcagaaaatg
    22321 aaataatcat tgaataaaaa ataatatcag gtcttaggcc tgatattatt cattaatgac
    22381 ataatgtact cgcgatagag aagatataag aatagtaaaa actgattgat aaaaaaggaa
    22441 aaaaatagta caataaatca aaaacgaggt tattatgggt aaaaaaaaga ttttaattgt
    22501 ggaggatgag caaaagattg cgcggttttt ggagcttgaa ttaaaatatg agggctatga
    22561 tgtagatatt gcaaacaacg gaagagaagg atttgaaaaa gggaaatccc ctgatgttga
    22621 tttgataata cttgatttaa tgctgcccgg attaagcggc attgaagtgt gcagaagatt
    22681 aagacaaaca tctgatgtac ccataataat gctgactgca aaggatgata tttctgataa
    22741 agtaacaggg ctggacatag gtgccgacga ttatatgaca aagccctttg ccgtagaaga
    22801 attattggcg cgcatcagag tattgttaaa aagaaaatac cataaaacca atgaacaaga
    22861 tatattgaaa atcgggaaac ttacactatg caaaaacaat tataaggttg aattcgacaa
    22921 tgaaaatatt gagcttacca aaaaagaatt tgaattattg gaatacctta tgaaaaacaa
    22981 aaacatagta atatcaagag aaaagatttt ggataatgtt tggggatatg attattatgg
    23041 agacacaaat attattgatg tatatataag atatttaaga agcaagatag accagaaata
    23101 taatgtaagt cttattgaaa ctgtcagggg agtaggctac ataatcagag attgaaggaa
    23161 gacaaatggt aaagacaatt cttagaaaac tgttaaactt aatactttac ccatttagaa
    23221 taaccgtaaa attgctaata agtctgttaa aaaggcttaa attttcaatt gcatttaaaa
    23281 tttctacaac atatttaatc ttgtacgtta taataattat gacagttgcc cttacaacct
    23341 ctgtaggcta tttaatattt gaactgcaga aatttgaaga tacaacggca gcaatcgatt
    23401 cagaaaatat aataaacagt ttcccgaatt ttaacactat aaatgtaaat gaaagaagct
    23461 ataattcttt tgaaatattt gacaataatc ttaacacata cttttcgaca ggaagaccgg
    23521 gaagatatgc ggacagcatt attgtcatta cggaaaaaat aatttttgac aatgaatatg
    23581 ttcattcgga aagctttaca aaaaacaatt ctatttatta tatgaatata aattacccga
    23641 tggaagatat gctcagggat gcgtggagaa taagctttat ggttttaata tccggagcca
    23701 ttggtcttat gtttttcgca ccaatagtat caagcacaag ttacaaactc atcggtccca
    23761 taaaaaacat gactgaaatt accaagacta tcacagtaaa caacataaac accaggctcg
    23821 acgttaaagg aacacaggat gaattgaagg aattgtcgca aaccttcaat gaaatgatgg
    23881 ataggataga aagcggatat aaaacacagc agcagtttgt ttcagatgca tcccatgaac
    23941 tcaggacacc gatagctgtt ataaagggct atgttaatat gcttgacaga tggggcaaag
    24001 atgataaagc agtgcttgaa gaatctattc tagcaataaa aaatgaaacc gaaaatatgc
    24061 aagatttaat tgaaaaatta ttatttattg caagaagcga tataagcact ttgaatttta
    24121 caaaggaaga tttcagaatt cgtgcccttc ttgaagaaat tgaaaaagaa acaaagatga
    24181 ttgacacagc gcacaaactg tattttaaat tttatgatga tgcaaccatt tacggcgata
    24241 agatgaggat taaacaggct gtgagaatac ttctcgataa tgccatgaaa tatactcctc
    24301 ccggcgaata tattatgatt tcaggcttca tccaggatga atactatgtt ataaaagtag
    24361 aggacaccgg tgtaggaatt aaaaaagatg atttaaataa aatatttgac agactttata
    24421 gggcagagca atcaagaagc aagcaagtaa gcggtcatgg actggggttg tcaattgcta
    24481 aaataatcgt attaggtcat aagggtaaaa taaaggttaa aagcacaccc ggaaaaggtt
    24541 ccgaatttaa attacttttt ccttatattg acagttgatt taaatttacc ataatcgaca
    24601 aaaaacatta aaataagtca aggaaatatc ctttccctag tagtataata gcttgtacat
    24661 tatgcagaaa ggatgatttt gtgaatattc agtttattga aacgggacaa gtattgacaa
    24721 tcaaattaaa gggcgagtta gaccatcaca gcgccgacga agctagggta ttaattgatg
    24781 aaaaaatcaa aaatgaaaag tacaataaat tggtaataga tttaaaaggt cttgatttta
    24841 tcgacagctc aggtatcggc tttgtaatcg gtcgatacaa agtcataaga aagcgtaatg
    24901 gtgtaataga aataataaat gcaggcaaaa aggtacgaaa gattttggac atgagcggca
    24961 ttggaaaaat aataaaaata aaataggggg acaaaatgca taataactac gtatatatta
    25021 aaataccaag tctatctgtt aatgaatcct ttgcaagggc tgcggtcgca gcattttgct
    25081 caagcttgaa tccaacaatg agcgaaatat cagatattaa gacagcagtg tccgaagcag
    25141 taaccaatgc agtggtacat gcctatgaag atgaaatcgg agaaattgaa attcaatgca
    25201 gaattaaggg gcaggtagtt gaaataatag ttgaggattt cggctgcgga attgcagatg
    25261 tagaaaaggc aagagagccg ttatttacca caagaccgga gcttgaacgt tccggaatgg
    25321 gtttttccgt tatggaatca tttatggatg aattggttgt tttgtcagaa aaggatgttg
    25381 gaacaaaagt tatattaaga aaaaaaatat ctgaaaaaaa acaaaagaca ttaaatgaag
    25441 aatttgatga tgaaaaatca gaagatgatt aataaataat taagcgaaat agaaattttt
    25501 atcttgaaca aatataaata tctatgtgac tcaaaaggta ctatttaaca tttctttaaa
    25561 aaaaaggcac tgttagtgct tttttttagt attcaaaaaa ctatttttgt aaataatact
    25621 tatgaggtga tttatatgaa aaaaaagata tttttattag taatactttt aattatcggc
    25681 ttaacagctg tttatttcgg cagcggaatt gacagatccc tgaaaagaat acctgaaagc
    25741 gcagtcttac tgtaatacct ttaattatac attaggaggt aaaatgaaat ataataaaga
    25801 caactataaa caagtagttg atgagtttac acctaaaaac aaaatgctta aaaactgtgt
    25861 gatggcattt atttttgggg gagccatatg tttggtggga gaaattatca atgactcttt
    25921 tgttgcctgg ttaggtatat cagatgaaga agcatcaccc atgacagctg tggtactaat
    25981 aggtattagt gcacttttga cgggtttggg ctggtatgat aaactaggca aaataggagg
    26041 agccggtaca gtcgttccaa taaccggatt tgcaaattcg gtagtttctc ctgccttgga
    26101 atttaagaga gaaggattga ttttaggtac ggctgcaaat atttttaagt tagcaggacc
    26161 tgtcttggta ttcgggtact tttcttcatt tatagcaggt ttgttaagta tgttatttaa
    26221 gtaaggagga aaaatgaaaa agacaggaaa aacttatata attaaagacg atgtagtaat
    26281 aagagatact tacaccatag tcggcaaaaa ggaaggacag ggacctttaa aagataaatt
    26341 tgatatggta atagaagatg atttatgggg agaagacaca tgggaaaaat gtgaattaaa
    26401 aatgcagaaa attgcagtag aaaatttgaa aaacaaaaat agtttaataa atgaaaacat
    26461 tgacctttta atttcaggag atttgttaaa tcaaatcaca tcttcttcat ttgctgcaag
    26521 agccttggaa ctgccataca taggagtata cggcgcctgc tccaccatga cattgacaat
    26581 gggtttaggc gcaatgataa tagacggagg atttgctgaa aatgtaattt gtgttacatc
    26641 aagccatttt tgcagtgcag aaaggcagta cagacttccg cttgaaatgg gaggacagcg
    26701 ccatatgtca gcacagtgga cagtaaccgg cgcagctgcg gtatggctct caaaaagcgg
    26761 aaagggacca aaaataaaac atgttacctt tggaaaaatc gttgatttag gtgtaaaaga
    26821 tgcaaataat atgggagctg ccatggcacc ggctgcttac gactcattaa atcagcatct
    26881 tcaggataca ggcattgatt atgatttaat tgttaccgga gatttgggaa cagtgggtaa
    26941 aggcatagtt aataagatgt tcaatgaaga taataagagt atcgaatcaa aatacgatga
    27001 ctgcggtaca attatttttg atattaacaa acaggatgta catgcgggag gaagcggctg
    27061 cggatgttca gcagcagtat tcggctcctt tttatacaag aaattattga ataatgaaat
    27121 aaaaaaactt ctgtttacag ctacgggagc acttcattcg cctactgcaa atcttcaggg
    27181 tgaaagtata cccggaatcg ctcactgctt gggtattgaa gtttaggagg gcatatgaat
    27241 tatttgatga gttttgtaat aggcggtttg atatgtgtag tcggccagat aataatcgat
    27301 acattcaaaa agaataatgc ttatctgctt gtgtttttcg ttgtgacagg tgcaatattg
    27361 ggattcttcg gagtttatga taagcttgta gaaatagggc attcgggagc taccgttcct
    27421 cttctcggct ttggcaactc cttagccaag ggagctatgg aagaagccgc aaataaaggg
    27481 tttatgggag cattgagcgg aggagtgata aaaacagcac ccggagttgc tgcagcattg
    27541 ttattcggat atatcgttgc tgttatattc aatcctcatg gtgataaata aaagagttca
    27601 gaatcgaaat tctgaactct tttatattca taattaccat tataaataat tgttattcca
    27661 taaagtgtca ttctgagggc tttagcccga agaatctcat gagatccttc gctttgctca
    27721 ggatgacgta tgagatcctt cgctttgctc aggataagcg accgacacca aatcaaagat
    27781 ttgggtcgtc tgcttaattt tctatggatt ccaatatttc atctatcggt ttatcagggt
    27841 cttcttccga attctcaaat tcatcgatta tctgatttat aatatcttcc aatgtatcac
    27901 cattgccgtt attgccgttg ccgtttccat tatggccatt gccgttatta ccgtttccat
    27961 taccattatt accgtttccg tcgccttcat tcaaccactg ttcataatgc catttggtgt
    28021 gataataaca agtccttgtt ggaacctgat actgatagtc ttcggttata aatccggaag
    28081 gacctaactt ttcaacttca attcctaaaa catgagttat ttgataattt tcatcaactg
    28141 acatctgacc ggcatatatt tgctgaagtt cttccatagt gaaaataatt ctgtcttcta
    28201 aaatttgctg atataatttt tttgcttcat agttatctgt cttttcttca gggtcaaaaa
    28261 gcgggtctct tgtaaagaac actctttctt ccaccaaatt acccggacag tatattgatt
    28321 ttttcatatt tgagctggta catacttcag ctttaacatg tacgtcgcaa gtttcagtag
    28381 gttgagttcc gggtataaaa tattccgtaa taatctgact tcctctctgg tcaagtgcac
    28441 aaaggtcacc cggcagcttt cctgatactt tacaaacact aattggtatt aggtttggat
    28501 ttcgtgtaaa tgttgcagga ggcagacctt gatgtatcgg agtcataatt gcactccaaa
    28561 gtcttgcgtt gttgccgctg tctccggaaa gcttcatttg aacattatca ttaccaaccc
    28621 aaattcctcc tacatagtat ggtgaaaaac cgacatacca gaaatctccg tttgcctgtg
    28681 ttgtacctgt ttttcctgca acttcgatac ccaattcctg gggaagtctt gccctatatg
    28741 aaagtccggg atttactgtt gttcttaaaa tatccttcat cagcgaagcg acttcaggac
    28801 tgtatgccac atgtgtttca gggatatttt ccagtattgt ttcaccctta gtattaacaa
    28861 ctcgcgtata tactatcggc tctacatata ctccatcatt ggcaatcgcg ccgtatgcag
    28921 cagtcattgc caatggagaa aatcctttgg taagtccgcc caaagcaaga gatgccaagt
    28981 tcacgtcatt gtaataagga tcttcttgcg gagatacaaa ggtatctttg tcagggttat
    29041 tttggtcgat tagtcttaat cttgataatg agtttatagc agcatcaagt ccgattgttt
    29101 caagcatggt aacagcattt gtatttattg actgctctat cgaccttcta agggtttgta
    29161 taccccagta ttttatattt ctgtgttcat accagttttt aggccatctt tcaccgcttt
    29221 gattgtattt tgggaggtca tcaattgggt aagcagctga atatcctaaa tcaagagccg
    29281 ccaaatatac cgataacggt ttgattgttg agcccggctg tcttgcagca tctacagcac
    29341 gattaaaggt cttactgcct tcaatctgcc ttccgccaat cattgcttta atttttcctg
    29401 ttttgtaatc gattattact gctgctgatt gaggctgtac aatacctttt tcctggaaga
    29461 agaaatatcc ttccggtatt tttaaaatac cgtcactccc tatattgaac atttccttat
    29521 ttttatccaa ataattttta ggtatcaaaa aggtcccttt tgatcctttt ttctcaatta
    29581 tttcatagtt atttcccaca ttgagggctc ctatggtgtg agaaacaaag ttcattttat
    29641 cgtcaattgt ataagcatca acgatatcta tagttgaaga atatatatcg aattttttag
    29701 agttaattac caagtttccg ttttcatcaa tgctgtactc atcaggattc aagtaaatgt
    29761 tgttttcttc atccattata ttttctttgg caaagaatat aagctggccg ctttcattca
    29821 ggatatttaa tcctgcatca agcattccgg ttccttcccc tcctgaccat ctgaaatatc
    29881 tccagtcctg ggcgataggt ttatctcctg taggctcggc acccaaatat aatacaggga
    29941 aattatcata agactgctct aaggattttt gaatattaac atcaattgta gaagtaatgg
    30001 ttaaaccgcc tttatacaga tagttttctg cttcctcata agacatgcct cgagtagcta
    30061 ttaagtcttc cacaaccttt tctttcacat agtccatagg tgttgatgaa ataccttcca
    30121 tctttgtctg tcccggattt aatgcaaccc gcatatcctc tgccatcgca gcatcatatt
    30181 cttcttgggt aatgtattct aattcaagca ttctgtttaa aatagtatgc tgcctgtcta
    30241 ttgcgttttg attgtacaca caggcatatt gaacagagcc gataaataca tttcctacta
    30301 tatcttcttc gggaatatcg cttatatcct ccaagttata acgattaaac ggtgcgtatt
    30361 tcaccgtact ttttgcagcc gctgctaaca atgcactttc agctaaggta agatcactta
    30421 catcttttga aaaataagca tatgatgcag cttgaacccc atattggctt tgccctagtg
    30481 gtatggtgtt aaggtaattt tctagaattt gttccttgga aagaactctt tccatcttaa
    30541 tagccaaata aatttccgtt atttttcgct cccaaacttt atcgctagta aggtataaat
    30601 ttttaacaag ctgttgggta atcgtgcttg caccttgtgc agtagggtct ccttctcgca
    30661 cattatgtat aacagctccc ataatacgtc ttatgtcaat accgggatgg tcttcaaaac
    30721 gatgatcttc tattgcaata aaagcatctt ttaagtattt aggtatttgt tttagcggta
    30781 ctatttctct gttttcgttt gggtcgtgga tttgctcaat aacatttccg ttttcatcta
    30841 cgataactga gctttccgta agagtaccaa gaacattcct tggatcgatt tcaggagcgt
    30901 ttcttattat tcccatcacc attcccagaa cggctccccc ggcaataatc actgccacaa
    30961 gtattaaaag aagggttact ctgataataa cctttctttt cttttttgcg gttctttttt
    31021 tcttgtcatt tttatttata gaatctgaat tattttcatt tatttcatta ttcttttttt
    31081 cttttgacat aactacctcc gcgtttgcat tagttagtat accacattta aagcaaatat
    31141 tgtcaaagat tttcatctta ataattatat aatagaaaaa gcttgaaagc aatagtttaa
    31201 aaattactca ggaaaaatta tgaaaattgt aagaaataaa gagtaattgc aaaaagacat
    31261 ttgtaatgtt atattatata taatcccgca gtatcattgc ttagggtata ttcaggatga
    31321 cataaaggaa ttttttagat tgctatatat tacggaggtt taatggaaaa agaaagatgt
    31381 ttgctggttg cagttcaact gccgggcgaa gaagaaaacg atatggaaga attggagcaa
    31441 ttggttgaag ctgcaggcgg cgaagttatt tcaagcattg ttcaaaacaa acaaagtatt
    31501 gacaacagat tttatgcagg tcaggggaaa attgaagaaa tagctaatta tgtgaaagaa
    31561 ttagatattg atacagtaat tttcaatgat gaattaagtg gttctcaaat acgaaatatt
    31621 gaggagattg tcgaagcaaa ggtaatagac aggactaacc tcatacttga tatatttgca
    31681 aaaagagcct taaccaagga aggacagctt caagtggaat tggcgcagtt aaaatacagc
    31741 cttccgagac ttattggtgt aaataaaaac ctgtctcgat taggcggagg aataggcaca
    31801 agaggaccag gagagcaaaa gctggaaatt gacagaagaa gaataaaaga aaaaatcacc
    31861 gatatacaaa atcagttgga agtattggaa aaggtaaggg aaaccaaaag aaagaaaaga
    31921 atgaaggacc aggtgccgat agtttccatt gtgggttata ccaatgcagg gaaatcaacc
    31981 ttgctaaatg cactgatgga taatgaatac agggaagaag gagaaaacaa gaaggttttc
    32041 gcccatgaca tgctttttgc aacccttgat acggagctta gaagagtgag attgcccggg
    32101 ggaaggagtg ctgtcttttc cgatacagta ggattcatta aaaagcttcc cacacaaatt
    32161 gtggaggcct tcaagggaac attggaagaa atcaaatatg ctgacttaat aattcatctt
    32221 attgacataa atgatgaaaa tttgtcggct cataaggaaa ctactattga gctgattgaa
    32281 aaaattacgg gaagtgaaat acctattctt accgtttata ataaggtgga taaaattttg
    32341 aatgaaaaag tatctgtttt aaacaatggt attatttaca tttcagcaaa atataaatat
    32401 aatattcaag agctgctaga agctgtggat aataagatta acggtccaaa aaacttttat
    32461 gatttaaaaa taccgaatga taatataaaa atttattact ggctttatga aaacagagtg
    32521 actgaaaatg tggaatttga cggtgacggt gtttcgctta gagttttatt gtacgaaaat
    32581 gaaaaggata agtacaaaga ttttatcgga gaaaaggatg agtaattata aatctatcca
    32641 taaagaagga agagacgaaa taattattaa taggtcaaga ttcattggta ctgcatgtcc
    32701 cgttgaaaca gaagaagaag cattggaatt tatagacaaa atcaagaaag aattcaagga
    32761 tgcgactcat aacgtgtatg cttatgtaat aggagaaaat tcaaacatac aaagattttc
    32821 cgatgacggt gagccttcgg gaactgcagg aatgcctgtt ttaaatgtaa taaagcagga
    32881 aaacattaaa aatgtcgcag ttgttgtcac aaggtatttt ggtggtgttc ttttaggagc
    32941 gggaggactt gttagagctt atacaaaaag ctcaaaaatt gctttggaaa gtggaaaaat
    33001 agtagacaag aaattgtttt atgatgtatt atttaagtta gactacacat tattagggaa
    33061 aatggataat gaacttttga aaaacaatgt tattatcaga gataaagaat atgaggaaca
    33121 agtgctgttt aaactgatag tcgaaaagga aaatttagac aaaatcaatg ccttggttgg
    33181 agagatttca agcggtaaag ctgaaatttc tgtgggacag gcaaaatact attccacaaa
    33241 aaatggaaag atagttgatt gattattgtc atcctgagcg aagcgaaggt cattgtcatc
    33301 ctgagcgaag cgaaggatct cataagatga taaaatgtca catcgcaatt aacagtaaag
    33361 taaggaggaa aatatgtctg gacattcaaa atggagtaat ataaaaaatc gcaaaggaaa
    33421 acaagatgcg ttaagaggta aaatatttac taagcttgca cgtgcaataa ctgttgcagc
    33481 gagagaaggc ggagcagacc cggattataa ttcatcattg gcaacagcaa ttgaaaaagc
    33541 aaaggcacaa aatatgccca atgacaatat agatagagca ataaaagcag gacttgcagc
    33601 atcggcagga gaaaactttg aaacaatcac ttatgaagga tacggaccga gcggtacggc
    33661 atttatggtt cagtgcttaa cagacaataa aaatagaact gcagcagaca taaggcatta
    33721 tttttccaag cataacggaa acttaggaac aacaggctgc gtaagctatt tgtttgataa
    33781 aaaaggtgta atttccattg aatcagacgg aattgatgaa gaagaactta tgatggcagc
    33841 acttgaagca ggtgccgaag actttgaagc ccaagatgac agctatgaaa taacaaccat
    33901 acctgaagat tttatccaag ttctaaacag tttaaaggaa gcaggctacg aaaatatcaa
    33961 aggagatatc atgtatattc ctcaaactta cgtgaaagtt gaagatgagg ctgcaataaa
    34021 aaaccttgaa aaattaattg atgcattaga agaaaatgat gacgttcaag aagtttacca
    34081 caactgggat gagcctgagg aagaggaaga ataagaatac taatttaacg aaattgcctt
    34141 aaattttata tgcatgaaac gcttaataat ttttaaggtt tttttatgca aactatggta
    34201 caatatagat agtaaatata aaatataata atatatagtt acacgattat aaaggagtga
    34261 tgtaaagtgt atgtaccata cggtttattc gatccgacaa tgctaatttt gattcctgcc
    34321 attatattta ctttgtatgc tcaaagtaaa gtgaaaacta attttgcaaa atatatgaga
    34381 gttcaaacac gaagaggtta cacaggagca gaagttgcca gaatgctttt aaaccaaaac
    34441 ggcttgcatg atgtacctat agaaacagca cggggtcaat taggagacca ttacgacccc
    34501 actaaacgtg ttttaaggct atccggggaa gtatatcaag gaagttccat agcatcagtc
    34561 agtgtggctg cccatgaagt gggtcatgcc atacagcatg caaacggct
//
LOCUS       DAOPUE010000009        32692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1281_length_32692_cov_10.296627, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000009 DAOPUE010000000
VERSION     DAOPUE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 32692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32692
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_32692_cov_10.296627"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKH42_01725"
     CDS             <1..200
                     /locus_tag="PKH42_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOA19176.1"
                     /translation="AGSFTNGMPMTSSIGCGTWGGNATSENVHLKHYMNVTWVSKEIP
                     EDRPTDEELFGEFYEPEREGK"
     gene            216..2033
                     /locus_tag="PKH42_01730"
     CDS             216..2033
                     /locus_tag="PKH42_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOA19177.1"
                     /translation="MKRLVADQMVEYLERRNVEYIFGLCGHTVIGFLDALSRSEKLKY
                     VSFRNEQLAAMAADGYARIKKKAGVVLCHLGPGLANTVTGVANASFDSIPMVVIAGDV
                     PRYYYGKHPHQEVNMHADASQYEILRPIVKRAWRIDDAEALPEILDKAFRLAESGRPG
                     PVLISIPMDVFSVKIDEELFNRTFKDSSETIKPALAPSAAKAIAKKLIEAKNPVIHAG
                     GGILLAGASEELRQLVEFLDIPVSRTLMGQGCLSDTHPLMLGQTGFWGLEFTHKFTAN
                     ADVILGLGTRFAEADSSSWYTGVTFDMNKTEFLQIDIDPAEIGRNYPVSIGAIADLKL
                     ALAQILEEAKALVPEGIKRPGLRDMIKANRDEFRKSNIEVSTDGRFPMTPQRILRDVK
                     SVIPEDAVIFTDVGWNKNGVAQQFDITIPGTIHHSSGLATMGFGGSALIGGKLAAPDK
                     VVLTLVGDGGFGVNPGALATAVEQNIPVVWVVMNNSAFGTIAGLEYSNYQTKFGTVFT
                     TPDGKPYSPNWAEVAKAYGVDAVKIKSADDFKPELEKAIKSNKPYLLDVPMENIAVPT
                     PGSWNINDIYNPKEGVVNGKLVKNEKGEYMPPDHSTSHK"
     gene            2055..2900
                     /locus_tag="PKH42_01735"
     CDS             2055..2900
                     /locus_tag="PKH42_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA19178.1"
                     /translation="MKRKFSLAYLTIPGTHPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFQFDLDRKLFKDIRDALEQNDIKLMDIELARVREDLKVEDYESAFAAAAELGATDV
                     LSSIWTKDKEFAFKEFAKICDLAAKYNLKVNLEFVTFSGVTDLTGALEVLDKVGRPNA
                     YLMVDTLHAHRSRVSPEDLAKVDRKKFGFIHLCDGPGEIPSLEDPSMIGVAREGRLYA
                     GEGEIDLKGMLLAMPDNPISIELPNSKEMKERGAAGHATRCLITAKELLVNMAKEEDI
                     ECQSI"
     gene            2885..3766
                     /gene="aroE"
                     /locus_tag="PKH42_01740"
     CDS             2885..3766
                     /gene="aroE"
                     /locus_tag="PKH42_01740"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOA19179.1"
                     /translation="MPVNIKTKMVTLLGKPLSQSYAARMQNAAYRAAGIDMLYFYTEV
                     ENDHLPDVVNGIRYMNFAGFAVTKPNKVEIMKYVDEKDPLCEKMNASNTVVILPDGRL
                     KAYNTDGIGFFRALKEEYPNTKIEESTFFCIGAGGAGRAICSVLAYYGAKKIYIANRT
                     LSKAQTLVDDINRNFAPVAELVDLNDTNLLKEKIKESDVVMNNTGLGMPSSLDGTPIP
                     KEYLHSGQLCFDATYNPSKTRFLIEAEEMGCSIMNGLGMSLYQGAEQIELWSGKTAPI
                     EAMRQELMNILAGKKEE"
     gene            3797..4375
                     /locus_tag="PKH42_01745"
     CDS             3797..4375
                     /locus_tag="PKH42_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOA19180.1"
                     /translation="MSAIKWLDEHLEEAIMVIMLALISCVMMAQIIARNVFNSMTWPE
                     EFSRYCYIWTVFLSLGYTIKKGNMLKVGLVMDLLPTKIRRSIEIVANLIILAICVVFF
                     RHAITYTGKIYNMGQISPAMHLPMWIMYMATVTGFGLASIRAAEEIVKNIKNFNVKVE
                     TTIEATLKEAMEEVETTGINVDDELKDSGGDE"
     gene            4375..5658
                     /locus_tag="PKH42_01750"
     CDS             4375..5658
                     /locus_tag="PKH42_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOA19181.1"
                     /translation="MALWIFVVFMVALIFAVPIAMSMVLGALTPLFLGGTGSSIQQLI
                     ANSFSGSDSTPILAVPLFILAGVLMAEGGISRKLFNFFAYFIGNFTGGLPCAVIITCL
                     FYGAISGSGPATTAAVGSMTIPFLVEMGYDKVWAAGMIATSGGLGVIIPPSIPFVLYS
                     LATGVSTGALFLGGVLPGIAIGLGMMVYAVYYCKKKGEDKEKINAKMKELRGKGFFNL
                     FKESFWALLSPVIVLGGIYSGYVTPTEAACISVFYSLFVSLIVYKSFTVKEIIPFLAK
                     AVKTYAPLCFVLAFAIAFGRVLALAKAPAIIEGFILDNFTTSITVLTGIVIVFLILGM
                     VMDTGPAIVILSPILLPAVQALGVHPVHFGVIMVCCLSIGLATPPFGLDLFVAGTLSD
                     TPPMTVAKKAIPFIIAFGIVLFVITYVPWLSLALL"
     gene            5698..6777
                     /locus_tag="PKH42_01755"
     CDS             5698..6777
                     /locus_tag="PKH42_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOA19182.1"
                     /translation="MKKLLILLLIATMALLPVACTQPSTPTPPEQPEQPEQPEQPEQP
                     GGFDALEPVVLIGADNAGVGAAAQLYGELITEKVKEITGGKLTIDYFPNSQLGNDQEL
                     QGQMLAGDIDFVICQTAQTVSFVPEVAIFDLPMVFAKYDAETIDYALNKSEFTDKINE
                     AYKAKNMYCLHFLQGGTFRETTSNKKIETIDDFNGLKIRTMENKNHMAFWQAMGAAPT
                     PLPWPEVYISLQQGLVDAQENATDTCVGANLQEVQDYLILTHHILYCNQFLINASKFD
                     SLDPLYQEALQQAIDEAAVEIEAQLTEIDVTNRQTLVDGGMELVEFDASFIDQVLEKA
                     KGVYESIGNDIGQEYVDSLLNALEK"
     gene            complement(6922..7797)
                     /locus_tag="PKH42_01760"
     CDS             complement(6922..7797)
                     /locus_tag="PKH42_01760"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOA19183.1"
                     /translation="MTLQQLHYFRVLAKVENYTKAAGILHIAQPSLSYSISQLESELN
                     IKLFERHGKKIKLSNPGELFLKYVEKSLDILDEGTIMLKTVADSSMGEVSLGYIYSIS
                     SKFIPEMIKKFYENNSNRLIKFSLVQNLGEQLIRDLKSNKIQLAFCPTPDKSLKCVKI
                     FSQQLHLIVPKKHPFSQRKCIEISDIIDEPFVALNKSSSLRSTVDEIFARYNFSPKIV
                     FEAEECNAVISFVSLNFGISIVPEIPSMNKHVNTVKICNMPLKREIYLCWIDDKYLSP
                     AAIKTRDFIIENFTV"
     gene            8199..9098
                     /locus_tag="PKH42_01765"
     CDS             8199..9098
                     /locus_tag="PKH42_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA19184.1"
                     /translation="MESVNKYKYMGFILLQSVIYGFGNPLTKIAFKSITPFWSLSVRF
                     TIAFIVFMLFFGRRIIGSLKSVEPKVYLPAGICMATAYITCNLALSWTSATNVGFLMS
                     LPVIFAPILALLIYKVKYRLIHIPVQIVVVIGLYLLCFDGEKLVFGKGEITALITAIS
                     IAGALVFGEESLKQLDAVAVSAVQAGITALISLPCALIFENTEILVHVTIPAWLVVLY
                     LAIFCTNIAYMLQNSALKYISASTVSMLQCTQPILTAIISYLLLREKLSTLGIIGAAI
                     IILCIIAENIITTKEQSASKNLV"
     gene            9154..9645
                     /locus_tag="PKH42_01770"
     CDS             9154..9645
                     /locus_tag="PKH42_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOA19185.1"
                     /translation="MFIKKNQFYIIIAVIVFLMAVYMIYSIRKDNRLNNNEENVIKLV
                     EDFGSTLKNVPLLAPRDEVIQAIKDNYSPYVTDELMQNWIAHPRRAPGRTTSSPWPDR
                     IEINEIERLSEDEYSIKGRIVEVTSTEAEGGGAAAFRPVELILKRAGNKWLIDKIIIK
                     NYE"
     gene            9851..10306
                     /locus_tag="PKH42_01775"
     CDS             9851..10306
                     /locus_tag="PKH42_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOA19186.1"
                     /translation="MAGLVPYNRRNRGLVDRGFSDFRDFYNMLDDFFYDAWPSKKYFA
                     RDTFKIDVQENDNEYLVEAELPGVNRDEVNIEMNEGRLNISIQREESINEENKNYVHK
                     ERRFSSMSRSLYLDDAKSTGIKAKLENGVLSIIVPKENKPNKSVKIDIE"
     gene            10554..11324
                     /locus_tag="PKH42_01780"
     CDS             10554..11324
                     /locus_tag="PKH42_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19187.1"
                     /translation="MSEVLRLEHVSKSFGSKDVLKDINFSVNSGEIIGYIGSNGAGKT
                     TTIKIILGLIDDYQGDVFIFGEKVKGQFEYKKRIGYVPEVSDMYDNLTAYEYLSFIGM
                     LYGIGEDKAVKKGKEMMSVFGIDDAINGRIHTFSKGMRQKLSIITGMIHNPDILFLDE
                     PLGGIDANSVLVFKEVLKNLKKEGKTIFYSSHILEVVEKLSDRILLINEGEIVIDGTV
                     ADVMSMQSDSSLESIFNYVTGFHDYEMLAKKYTDVFMS"
     gene            11336..12949
                     /locus_tag="PKH42_01785"
     CDS             11336..12949
                     /locus_tag="PKH42_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19188.1"
                     /translation="MNEFLSLKILDSFKRIFEGFCIDYKLLRIILKTKLILDSRRAPV
                     INTNQKKKDEEKVSLLKGYFMHLLFGAFLAFFIYFIKDEMIRMMYFSGAFFFMTTMYL
                     ISDFSFVLLDTKDKNILLTKPVNSRTVSLAKVLHILIYMFRLNTFIAGPSLIALVMSN
                     DLFVIPIFLVEILLMDLLIFLVTAFIYLLVLRFFDGELLRNIINFIQILLTILVTVGY
                     QIAIRMIDFTSLRNMEYTFNSRHFLNPILWFGAPFEIFYHGGESYLYVFTALIIIVPL
                     VSFIVYLSLSKKMESLLLKLEGEGKERPSIHWIEKIVGFIINPNEISKQSFNFANAMF
                     RSDRSLKLKIYPALSSGILLPILMIFNMRMGGREFKPSGYEYLYLYFNLLIFSSVFYL
                     VKYSSAWKGSYVFISSGFKDYKNLYRGLMRSMAYRFFIPLIILNGVVFTLIFKFRFVE
                     DIVILFLAGIAILPLIGKIYLEIPFSKPIEESNQRDSFDKFLLSFAIVAIFAIVHFYS
                     NIIPMGKWIYLSVLAVGIPISWKFATPGE"
     gene            12968..13543
                     /locus_tag="PKH42_01790"
     CDS             12968..13543
                     /locus_tag="PKH42_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HOA19189.1"
                     /translation="MDKVTLWTRQDIRSLDDLERKGVIRITRKHLEEKFDVISDHIIF
                     LYNWFVNEAEKRVPRPEGVEFFIWCSISEEYMLRPTLNEIVYVLEVDKSEVVYFDGMK
                     WDYVLNHHYVPKDEKDAEEYLKDLERKGFKNSYSFINEKTAHFYPQERKRVMDSWARV
                     FEIDEWDIFRIQANIWEIRPEMIKDILRADS"
     gene            complement(13540..14160)
                     /locus_tag="PKH42_01795"
     CDS             complement(13540..14160)
                     /locus_tag="PKH42_01795"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOA19190.1"
                     /translation="MTKYKSDNVHNRTMTLTGHSEMEIAPDIVIIRLGVQTTGEDLSQ
                     IQADNARISQSIIQTLQRMGITDIKTSQYSIDRVYDFEDGRQIDRGFSVRNILQIRTT
                     NLEAAGSIIDAAVNAGANVVDLITFDISNREYYYQQVLNMAILNAIQKANSITMNLGY
                     STPALVVNIVENAAIPFLPVQREFAATPIIPGTIRIEANVTVDFVF"
     gene            14376..14747
                     /locus_tag="PKH42_01800"
     CDS             14376..14747
                     /locus_tag="PKH42_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA19191.1"
                     /translation="MKFLWTTILVNNMDESLKFYEEIVGLKMTERFQAGPGMEISFLG
                     EGETKVELIYNEKFKKLDAGNRVTLGFKVESLDEKINFIKEKGIKIITGPIEPNPSIK
                     YFIIKDPDGVKIQFAQQLQSN"
     gene            14931..15659
                     /locus_tag="PKH42_01805"
     CDS             14931..15659
                     /locus_tag="PKH42_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOA19192.1"
                     /translation="MLRIAICDDDKELCIELKSMLNEISKNTGEAFEISVFCNGEGLC
                     DSLMSGNRFNLIFLDIELCEINGVEVGKKIREELNDEITQIVYISAKESYAMELFDIR
                     PLNFLVKPLKIEKIKYVLKTARKVLGSSNQYFEYRVGNVNFNVLISDILYFESSGRKV
                     KVVLKNEVNEYYGKLSEVEEKIKNDDFFFIHKSYLINYNHVIEYSYEYVKMSNNEILG
                     ISQNNRKAVREKLLRKKQRMRHVK"
     gene            15649..16920
                     /locus_tag="PKH42_01810"
     CDS             15649..16920
                     /locus_tag="PKH42_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOA19193.1"
                     /translation="MSNSYVAVYLITNMFATYTLYRYMCIFFDRKDVDKRQEFLSYSL
                     YFCINSFLYLTFNKPALNIASNLIMFFLLTYNYQSTTKTRLIAVVLIYMILMTIEVLV
                     MLILQNYGITVTSGNDAALITGLISIKIISYIFMLFMSNFKMINNEIKVSNIHWLSIF
                     IIPVGTLILVLIMISMDYASNVTGIIISIMILLIINVFVYYFYDELMKSYQSKIEKTL
                     LQQQNNAYLKQLEIINQSKENIKTFRHNVKNHALTLKYYIDNHDRDGAIKYLDNIFEF
                     INYSKEHAKSGNFEIDSLINYKMDLAEKNNIKAEVYLAIPHKLNINPFDLNIVIGNLL
                     DNAIEAALKAENKFINISIELDRNVLYISISNSYDGRLKLIKNKLVTTKDNDNHGIGL
                     SSVQNSIEKYNGTMNIHYDERVFYVDVLMYN"
     gene            17072..17470
                     /locus_tag="PKH42_01815"
     CDS             17072..17470
                     /locus_tag="PKH42_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19194.1"
                     /translation="MNFEEYIRKVYTIAFRLTGDETSAKDVASLAIDKNANIVKENID
                     SNILHITIKEVCGIFLKEYSIFSSESFYNKNNTTIQNKLMNLEPLSRTAVVWKDIMGY
                     NMKDLETISNCPKSELYEKLNKARKNLIHF"
     gene            17553..18230
                     /locus_tag="PKH42_01820"
     CDS             17553..18230
                     /locus_tag="PKH42_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003207775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOA19195.1"
                     /translation="MKYKCLILDHDDTAVKSTPNIHYPSFVEALKSLRPDIELSLNDF
                     VNYCFNPGFSLLCKDILKFSDTEQQYQYKIWKDYVNNNSPEFYSGFPELIKEYKSLGG
                     TICVVSHSESQHIIRDYTKHCGLKPDLIFGWELDEHQRKPNSYPIIETIKRFNLRNTE
                     MLVLDDSKLGLDMARSCNVEFAAAGWSHSVPGIEKYMRENSDYYFSTIDEFRKFILDC
                     GNLYDFK"
     gene            18346..18573
                     /locus_tag="PKH42_01825"
     CDS             18346..18573
                     /locus_tag="PKH42_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOA19196.1"
                     /translation="MSVEKLLLSNLSTIEAEIVIAKLKSYGIPVLKKSKGTGELMEIY
                     TGANMYGIDLYVPSDMIELAEELLKPENETE"
     gene            18643..18894
                     /locus_tag="PKH42_01830"
     CDS             18643..18894
                     /locus_tag="PKH42_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19197.1"
                     /translation="MMFKTWIWLLIIAVLCILLIYVAKKKTKWFGLMLSILFFVVSIF
                     IVVYSKSMTSSYLILFSIFIIANIPTAIQLYIFFKYPEK"
     gene            19017..19907
                     /locus_tag="PKH42_01835"
     CDS             19017..19907
                     /locus_tag="PKH42_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA19198.1"
                     /translation="MKKYKVWILLTSCVLFWSGNYVFGSLVVTELTPVGLTFIRWLFA
                     SVILLIVGQKIEKPDWKEVCKQWPKITMLSMTGIIGYNIILYYALYYTSPLNAALVNS
                     INPSFIAIVSAFYLKERITKYHVFGIMISFLGVWIVLTKGNLLQAFNMKYNRGDLLVI
                     AAVITWTSYSVISKKLTTVPPITSTGVSGIMAGIILFPFAMTSGVNYLNMSTLALTAV
                     VYIILFPSLLSFVFWNIGVREIGAGKTGIFLNLNPVFTAFISRAMGKDITWVQVIGGL
                     LVFTGVYITTAFKGKTARLK"
     gene            19918..20433
                     /gene="cobO"
                     /locus_tag="PKH42_01840"
     CDS             19918..20433
                     /gene="cobO"
                     /locus_tag="PKH42_01840"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOA19199.1"
                     /translation="MEKGYIHIYTGNGKGKTTAAFGLAVRAALSGKKVFIGQFVKNME
                     YNETKIKDYLSNIEIKQLGRGCFIYEDPEEIDKETAKKGLEECSTLLASGEYGLIILD
                     EINIALYFKLLEVSDVVSALKNKAENTEVVLTGRYAPKELIEIADLVTEMVEIKHYYS
                     RGVLSRDGIDH"
     gene            complement(20486..21289)
                     /locus_tag="PKH42_01845"
     CDS             complement(20486..21289)
                     /locus_tag="PKH42_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA19200.1"
                     /translation="MKKSEIKPEEGKKPSPKSPKYTEVNSKFIDKWIDEGWEWGQPVS
                     HEVFERAKNNDWFVLLTPTKPVPKEWFCEMKNAEVLGLASGGGQQMPIFTALGAKCTV
                     LDYSEKQIASEIEVAKRENYEIKTIRADMTKPLPFEDETFDLIFHPVSNCYIEDVLPV
                     WKECFRVLKKGGILLAGLDNGINFIFDDDETTPVRKLPFNPLKDEELYQLSVEKDWGI
                     QFSHTIEEQIGGQIKAGFIIEDIYEDTNGSGRLHELNMPTFYATKAVKK"
     gene            21497..21871
                     /locus_tag="PKH42_01850"
     CDS             21497..21871
                     /locus_tag="PKH42_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA19201.1"
                     /translation="MNIIISNSSGQPIYEQIASQIKSMIITGELREGEALPSMRLLAK
                     ELRISVITTKRAYDELEREGFIVSFTGKGSFVAGKNIELIKEQHLKEIENHMQEILKL
                     SKECGLKLDEMIEMMTLLYEGE"
     gene            21873..22721
                     /locus_tag="PKH42_01855"
     CDS             21873..22721
                     /locus_tag="PKH42_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19202.1"
                     /translation="MEYSLKLENVTKVYKDFKLDNINITLPKGSIMGFIGENGAGKTT
                     TIKLILDLISRDGGNISILGKDNKTEMDSVKENIGVVLDESSFPENLNAREINNMLKK
                     VYKTWDEGKFSKFLDMFSLPYNKSIKDYSRGMKMKLSTAVSMSHDSKILILDEATSGL
                     DPVVREEILDTFLDFLQDETNSIFVSSHIISDLEKICDYITFIHKGKIIFSETKDDLL
                     DNYGILKCSPEEYENIDKSLVKGMRKNKFGIEALVLKDGITGPYLIDRASLEDIMIFI
                     IKEQVQ"
     gene            22718..>23293
                     /locus_tag="PKH42_01860"
     CDS             22718..>23293
                     /locus_tag="PKH42_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOA19203.1"
                     /translation="MTGLILKDLLNLKKQGKVYLILIIFYFAIGLVNENSSMFASMMT
                     MVAVLIPITAMAYDERCKWDRYALTMPVSRKDMVMSKYLLGLIFLTAAFVISILLNMV
                     FSNMPINEKILTALGNFFAGLTLMAVIFPLLFKFGVEKGRILMMIVIFIPVAAVMMIS
                     KIGIPMPAEEKLEQLIYFLPIIGVLLFIISIY"
     assembly_gap    23294..23393
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23394..23649
                     /locus_tag="PKH42_01865"
     CDS             <23394..23649
                     /locus_tag="PKH42_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOA19204.1"
                     /translation="YEFDAVIKKVPDIDGAYIEFPYDVKEEFGKGRVKVYATFDGEPY
                     VGSLVRMGTPCHIIGIRKDIRAKINKQPGDIVHVTIRERS"
     gene            23831..26173
                     /locus_tag="PKH42_01870"
     CDS             23831..26173
                     /locus_tag="PKH42_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOA19205.1"
                     /translation="MDFKKFMVLFLCILLFASCSDNNVVKEPEPVEEPEVIEEDNIPT
                     AWNLSMEEFRVDVPFSVPDVIPVVEKYEVNEDLSNLVNAGQYAGFTDKQLKSIYEDGF
                     VVLKPSYEYLKMHHLYEYPMYKESPVFITVDSALHLYHIFYGNSLKLLEVSSLYDKLQ
                     SLSKNMLIESLNAYNDSKYANLKEELKFAAAYFLTGAKLIDEDLEGIVVPEEIAVLSD
                     DEIKLIDEAFDFARSPIFGKDLDYSQFTVRGHYTGNEELGKYFKTMMWYGLSGFPIFD
                     ESKSKPVLDMDSLTKSMIITCLLLRNEDSFDDFENIYTATALYTGMSDDLGIFEIRDL
                     ITKVYGQNPDLNKFKDNSYYDKLLGEALLLPEPKIQPKYSSVSTPAGRQFRLMGQRYS
                     FDAEVMQALIEPIIRPIPSGLDVIASFGSKRAEELLDTYYKPKEDWDKYEENLNLMRK
                     KQTEITDDEWKSDLYKGWLWSIKSSAVSFEDKEGMPHFMRNEKWTDKNIHTALGSYAE
                     LKHDSILYMKQSGAEMGGGPEPIIPYNYVEPNVEVYAKLKWLAENTKAQLQERNMLKD
                     EIGLVLDQIIDIQDTLMNVSVKELTNQDITDEENLKLYRYGGLIDSVIQIMQMNLMRN
                     DVDTSNDFTTALIADVSTIAPNDLFPKGTYLEIGNGLPCEIYVVCQTNGKTYLARGAL
                     FNYYEFLSDKRLTNHEWQTLVGVKRMAMVYDEEKNIHVPMDIYDEDGNRILEEDEYDF
                     ENIMIIGPSENMVPKPAWTESFISQEENKVTIKDISISWE"
     gene            26175..26897
                     /locus_tag="PKH42_01875"
     CDS             26175..26897
                     /locus_tag="PKH42_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19206.1"
                     /translation="MKRLLIPLAVIMAGAVCVLIRQPESYAAGKISIEQNTYGLYEKS
                     NYLYIKSGKKYVKIIDEEIIDFKTYDIDSDKDDEILAISKHISEEYGSDFIIYDIYEA
                     DGIILTKEIYRQDFFDFKPWKVDACNLDNDGETDIFIGVYKDTVFYKDLRKRPFFYSW
                     DGDRLHKKWLGSFFSDWDLVDIAFGDFYNLGYDVAAVLEKNKDACRIGIYQFIGFGFE
                     NMKTINSVDNSFRSQTELLFGK"
     gene            26998..28224
                     /locus_tag="PKH42_01880"
     CDS             26998..28224
                     /locus_tag="PKH42_01880"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA19207.1"
                     /translation="MKRFFTRKKVIVFVVVAALAAIIFWLVNKGSKAPEVTANVSKLE
                     RKTLEHVISVKAPLVGIEKAEVTSPLNYKINDIRVKEGDLVKKDDILAVLDSEDLEKE
                     IEYEENQIELSKMEAEEKIKALQIEYDKALASYRDMETVYEQNKDLYENGVITEEAFK
                     KTEKDLVVLKKSLEGYNAVNGKIQLSQADKKRIEIQEDRLKLKKEELEKVMIRSSIDG
                     TVTRVNVNIGRYAKDTENSQAMFVVENLEKLQMKAAVSEFDVGKLKLNQEVEIYSDIL
                     GKGFAKGKVARISPTAEQKDNNTMERVIPVLIDVTERPENLIAGVIATAKIRTEKKEN
                     VFAVPSGAIVQDGEEYKIYILNEDNTVRSIPVEIGLETDLESEITGDLYEEMKYVINP
                     DETFEDGMTVIPNEIE"
     gene            28231..28938
                     /locus_tag="PKH42_01885"
     CDS             28231..28938
                     /locus_tag="PKH42_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19208.1"
                     /translation="MEKMIRIENLNKIYKNGSVEVHALNDVNMEVSRGEFLAVMGHSG
                     SGKSTLMNIIGCLDRPSSGKYFLDGIEIDKQSPDELSLIRNKKIGFVFQAFNLIPRTN
                     VLSNVELPMIYAKVKSKQRSERALELLEKVGLKDRINHMPNELSGGQMQRAAIARALA
                     NNPPIILADEPTGNLDTNSSEEIMKIFKDLNREGTSIIVVTHEPEVAKYADRIIVFKD
                     GCIIEDRWKEGGRNYLA"
     gene            28856..30133
                     /locus_tag="PKH42_01890"
     CDS             28856..30133
                     /locus_tag="PKH42_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19209.1"
                     /translation="MQTELLCLRTDALSKTDGKRVEEIIWLENIKIAFKSILSNKMRS
                     LLTMLGIIIGISSVISIVSIGDSMKGVMDDIYKNFGKNRAVLYVGNIEDFRTTDFFYN
                     DDLEILKERFSEKLAYICPSDSLRSDVTAGRNTIKLNMEFIDSGYDKVQQIKMLYGRM
                     INSKDIERKSKVVVLEQKAALKLFGKDNAVGKTIRVKIDNNMEDLLVVGVYKIEQSPL
                     LTLMQGQSNYENSFIPYTMPTSSYYAYYALDIFIDEKYSMAITGDEIINYIARMKNRT
                     PENYIYYTVEEEQTQVESVVTGLSVAVAAIAAISLLVGGIGIMNIMLVSVTERTREIG
                     IKKALGARTKDVLFQFLIESATLSAIGGIVGTITGIGLVMIGGSFISIPVVVNPASII
                     LAVVFAMVIGIFFGYYPAKKAANSDPIEALRYE"
     gene            30164..31537
                     /locus_tag="PKH42_01895"
     CDS             30164..31537
                     /locus_tag="PKH42_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOA19210.1"
                     /translation="MILSEEQKNHYKRQIIIPEIGEQGQKKLLDSTVLVYCENTDSLR
                     PLAYYLAAMGIGKIFCDLQDKIGADSLFAEVIDLNNDTKIDYLNEENYSGGFSEIYEN
                     NKNHPQNFCRIVLGNYSFIKNSAAKLLNDKYLPTIISANSQWKGTLQTFINEDLFKNF
                     IALIADNNTKDFSPPFLANALSATLSSIECVKLCLNIGNITKDMLFFDLFNMEFKQTD
                     NNANFFEFLNEANKMDIKQKLSDAKVLIVGVGGLGSPAAYGMAMADVNTLGLLDLDEV
                     EMSNLNRQILHSFSRIGMPKASSAEFMLKKLNPSINIKTYKTELTRDNAEKIFLEYDL
                     IIAAVDNIPSRYLINDICFLLNKPFAEAGVLRFDGTATTLVPKEGHCYRCLYPNVDSS
                     NLSGNNGVLGSVPGVMGFIQAAEAVKILSGFGKTLKNKILLFDSLAMDFNIIDIEKNP
                     RCPTCGK"
     gene            31664..32107
                     /locus_tag="PKH42_01900"
     CDS             31664..32107
                     /locus_tag="PKH42_01900"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA19211.1"
                     /translation="MKKFKKFTIIFCALMLLLVACAPGDGNLNEDEDNEPIDEPIDDN
                     NTGANANNDTFDEEEKTLTGSAQGYGGEINVTVVVNGEDIVSVEAEGKDETEGVGSLA
                     IDELPDKIAQANSTDVDNVSGATKTSEGIKQAVNNALQSNKQSKQ"
     gene            complement(32144..32578)
                     /locus_tag="PKH42_01905"
     CDS             complement(32144..32578)
                     /locus_tag="PKH42_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOA19212.1"
                     /translation="MDFLDMQIIKHLEKEGRISHEELSKRLNLSRPAIHNRVAKLEDQ
                     GIITGYKAMIDWSKLGFTTYAIISLRVRTADYDKLLEQLKNLAIPDLVIEECHIVTGT
                     WCILMKVRCSAPSGLKAIQDELHKIECIRESSTSLILSSLYD"
BASE COUNT        11177 a         4949 c         6580 g         9886 t
ORIGIN      
        1 atgccggctc cttcacaaac ggaatgccca tgacatcaag cataggatgc ggcacatggg
       61 gcggtaatgc aacatcggaa aatgttcatt taaaacatta tatgaatgtt acctgggttt
      121 ctaaagaaat acctgaggac agaccgactg atgaagaatt gttcggagaa ttttatgagc
      181 ctgaaagaga aggaaaataa ttaaggaggt aatatatgaa aagattagtt gcagaccaaa
      241 tggtagaata tttggaaaga cgtaatgtag agtacatttt tggtttatgc ggtcatacgg
      301 taattggttt tttggacgca ttatcgagaa gcgaaaaact aaagtatgta tcatttagga
      361 atgagcagct tgctgctatg gctgccgacg gttatgcaag aatcaaaaag aaggccggag
      421 tagtactatg ccatttaggc ccggggttgg ctaataccgt aaccggagtt gccaatgctt
      481 cgtttgattc aatacccatg gttgttattg caggagatgt tccaagatat tattatggaa
      541 aacatcctca tcaagaagtt aatatgcatg cagatgcttc tcaatacgaa atactaagac
      601 caattgtaaa aagagcttgg agaattgacg atgcggaagc actgcctgaa attttggata
      661 aggctttcag gcttgcagaa agcgggagac cgggaccggt gcttatatca atccccatgg
      721 atgtattttc tgttaaaata gatgaagaat tatttaaccg tacatttaaa gacagctcgg
      781 aaacaataaa accggcactg gcaccttcag cagcaaaggc aatagctaaa aaacttatag
      841 aagccaaaaa tcctgttatt catgcaggag gaggaatact tttggcaggt gcatcagagg
      901 aattaagaca gctggtagaa tttttggaca ttcctgtttc aagaacactt atggggcaag
      961 gatgtttgtc ggatacccat cccttgatgc ttggtcaaac aggattctgg ggtcttgagt
     1021 ttacacacaa atttactgca aatgcagatg taatacttgg attaggaaca cgattcgccg
     1081 aagcagacag ttcaagctgg tatacgggtg ttacttttga tatgaataaa acagaatttt
     1141 tgcagataga tattgacccg gcagaaatag gaagaaatta tcccgtatca atcggtgcaa
     1201 tagcggattt aaaacttgcc ttggctcaaa ttcttgaaga agccaaagcc cttgtaccgg
     1261 aagggattaa aagaccgggt ttaagggata tgattaaagc aaatcgtgat gagtttagaa
     1321 agtcaaatat tgaagtttca acagatggca gattcccaat gacacctcaa agaattttaa
     1381 gagatgttaa atcggttata ccggaagatg cggttatctt tacggatgta ggctggaata
     1441 aaaacggagt tgctcagcaa tttgatatta ctataccggg aacaatccac cacagttcag
     1501 ggttggcaac tatgggattt ggcggctcgg cgcttatagg cggcaagctt gcagcaccgg
     1561 ataaggttgt attaacactt gtaggagacg ggggattcgg agttaatccc ggagcattgg
     1621 caactgcagt agagcaaaac attccggtag tgtgggtagt aatgaacaat tctgcatttg
     1681 gaacaattgc gggattagaa tattcaaatt atcaaacaaa attcggaaca gtattcacaa
     1741 cacctgacgg taaaccttat tcgccaaatt gggcagaggt tgccaaagct tatggagttg
     1801 atgctgtaaa aataaaatca gctgatgatt tcaaaccgga acttgagaag gcaatcaaga
     1861 gcaacaagcc atatttgttg gatgtaccga tggaaaatat tgcagtgccc acaccgggaa
     1921 gctggaatat aaatgatatt tataatccta aggaaggtgt tgttaacggt aaattggtaa
     1981 agaatgaaaa aggggaatat atgcctccag atcattcgac atcacataaa taagtgttac
     2041 caaaggagga taatatgaaa agaaagtttt cgctggctta tttgaccata ccgggtactc
     2101 atccggttga tcagataaga attgccgctg aggcaggata tgattttgtg agtttaagac
     2161 caattcctat gcatcttcca aatgagccat tgtttcaatt tgatttggac aggaaattgt
     2221 tcaaggatat aagagatgca ttggagcaaa atgatataaa gcttatggat attgagctcg
     2281 cgcgagttag agaagattta aaagttgaag attatgaatc agcatttgct gcagcagccg
     2341 agctaggtgc aacagatgtg ctctcaagta tctggactaa ggataaggaa ttcgctttta
     2401 aagaatttgc aaaaatttgc gatttggctg ctaaatataa tttaaaagtt aatcttgaat
     2461 ttgtaacatt ttcaggagtg accgatctta cgggggcttt agaagttctt gacaaagtag
     2521 gtagacctaa tgcatactta atggtagaca ctctgcatgc tcataggtct agggttagtc
     2581 ctgaggattt agctaaagtt gacagaaaga agtttggatt tattcatctt tgtgacggtc
     2641 ccggtgaaat tccgtcctta gaagaccctt ccatgattgg tgttgccaga gaaggaagac
     2701 tatatgcggg agaaggagaa attgacctta agggtatgct tttagccatg cccgataatc
     2761 cgatatcaat agaactgcca aattctaagg aaatgaaaga aagaggagca gcagggcatg
     2821 ctacaaggtg tctcattacg gcaaaagaat tattagttaa tatggctaaa gaggaggata
     2881 tagaatgcca gtcaatataa aaacgaaaat ggttacattg ctgggaaaac ctttatccca
     2941 gtcctatgct gcaaggatgc aaaatgcagc ctatagagct gccggaattg atatgttgta
     3001 tttctatacc gaagtagaaa atgaccacct gcctgatgtg gtaaatggaa taaggtatat
     3061 gaattttgca ggttttgcgg ttacaaaacc aaacaaggtt gaaattatga aatatgtgga
     3121 tgaaaaggat cccctttgtg aaaagatgaa tgctagcaat acggttgtta tattacctga
     3181 tggcaggcta aaagcataca atacagatgg gataggtttt ttcagagcct taaaagaaga
     3241 atatccgaat acaaaaattg aagaatcgac atttttctgt ataggtgcag gcggcgcggg
     3301 aagagcaata tgcagtgtgt tggcttatta cggtgccaaa aaaatatata tcgcaaacag
     3361 aactttatca aaagctcaga cattggttga tgatataaat cgaaattttg cacctgtcgc
     3421 tgagttggtt gatcttaatg acacaaatct tctaaaagaa aagatcaaag agtcggatgt
     3481 tgttatgaat aatacaggac ttggaatgcc ttcaagcctt gatggaacac ctatacctaa
     3541 ggaatatctt cattccggac aactatgttt tgatgcaacc tataatccgt caaaaactcg
     3601 ttttcttatt gaggctgaag aaatgggatg cagcataatg aatggtttgg gaatgtcttt
     3661 ataccaaggg gctgagcaga ttgaactttg gagcggaaag accgccccaa ttgaagcaat
     3721 gcgtcaggag cttatgaata tccttgcagg gaaaaaggaa gagtaatgca aatttccaac
     3781 aaaaggagga aatataatgt ctgcaataaa atggttggat gagcacttag aagaagccat
     3841 tatggtgatt atgttagcac ttatatcatg cgtaatgatg gctcaaataa ttgctcgaaa
     3901 tgtttttaac tcaatgacct ggcctgagga attttcaaga tattgctata tttggactgt
     3961 ctttttatcc cttgggtata cgataaaaaa aggcaatatg ttgaaggttg gacttgtaat
     4021 ggatttattg cccacaaaaa taagaaggtc tatagaaatt gtggctaact taataatatt
     4081 agcaatatgt gtggtttttt tcagacatgc tatcacctat accggtaaaa tctacaatat
     4141 gggacagatt tcgccagcaa tgcatttacc catgtggata atgtatatgg caactgttac
     4201 aggttttgga ttggcttcaa ttagagctgc tgaggaaata gtaaagaaca taaagaattt
     4261 taacgtaaaa gttgaaacaa caattgaagc tactttaaaa gaggctatgg aggaagtaga
     4321 gactaccggt attaacgttg atgatgaact taaggattca gggggtgacg agtaatggct
     4381 ttatggatat tcgtagtatt tatggttgca ttgatatttg ctgtacctat tgccatgagc
     4441 atggtcctgg gtgctttgac tccgcttttt ctcggcggta caggcagctc aattcagcaa
     4501 ctgatagcta acagcttctc aggctcggat tctaccccta tattggcagt acccctgttt
     4561 attctggcgg gagtacttat ggctgaagga ggcatatcca gaaaactctt taatttcttt
     4621 gcttatttta taggtaattt tactggagga cttccttgcg cagtaataat aacttgtttg
     4681 ttctatggag caatttccgg ttcaggacct gcaactacag ctgcagtagg ctccatgacc
     4741 atacccttct tggttgaaat gggttacgat aaggtttggg cagccggaat gattgccaca
     4801 tccggtggtc ttggcgtaat tataccgcct tctatcccct ttgttcttta ctcattggca
     4861 accggtgttt ctacgggggc acttttcttg ggaggtgtat tgccgggcat agcaattggc
     4921 ttaggcatga tggtttatgc cgtatattat tgtaagaaaa aaggagaaga caaagagaaa
     4981 atcaatgcaa aaatgaagga gcttagaggc aaaggatttt ttaacctgtt taaagaaagt
     5041 ttctgggcat tactttcacc ggttattgta ctgggtggta tttattcagg ttatgtaaca
     5101 cctacggaag ctgcttgtat ttcagtgttt tattctttgt ttgtttcatt gattgtatat
     5161 aagtcattta cggttaaaga aataattcct tttttggcaa aagcagtaaa aacatatgca
     5221 ccgctttgct ttgtactcgc gtttgcaatt gcatttggaa gagtcttggc attagctaag
     5281 gcaccggcta ttattgaagg attcatttta gacaatttta caacaagtat taccgtattg
     5341 acgggtatag ttatagtatt tttaatactt ggaatggtta tggatacggg acctgccata
     5401 gtaatattat cacctatatt actgccggca gttcaagctt taggagtgca tcccgtacac
     5461 tttggtgtta taatggtttg ctgcttatcg ataggtttgg caacaccacc atttggattg
     5521 gatttattcg tggcggggac tttatcagat acccctccaa tgaccgttgc aaaaaaagct
     5581 atacctttta taattgcatt cggtatagtg ttgtttgtta taacatatgt accatggctc
     5641 agtcttgcac tcctgtaaag gatcaagtat atatttaaaa agaaaggaag gagaaaaatg
     5701 aaaaaattat tgatattatt gttgatagca acaatggcgt tgctgccggt agcttgtact
     5761 caaccgagta cacctactcc ccccgaacaa cctgaacagc ctgaacaacc tgaacaacct
     5821 gaacaacctg gaggctttga tgcacttgaa cctgtagtct tgataggtgc tgataatgca
     5881 ggagtcggtg ctgcagctca gctctatgga gaacttatta ccgagaaggt taaagaaata
     5941 accggaggta agcttacaat tgattatttc ccaaacagcc agcttggaaa tgaccaggaa
     6001 ctccaaggtc agatgcttgc cggagatatt gacttcgtaa tttgtcaaac agcgcaaacc
     6061 gtatctttcg ttcctgaagt agcgattttt gaccttccga tggtatttgc aaaatacgac
     6121 gctgaaacca ttgattatgc attaaacaaa agcgagttta cagataaaat aaacgaagct
     6181 tacaaggcaa aaaatatgta ctgtttgcac ttcctccagg gaggaacatt cagagaaaca
     6241 acatctaaca agaaaattga gacaattgat gatttcaacg gcttaaagat aagaacaatg
     6301 gaaaacaaga accacatggc attttggcag gcaatgggtg ctgcacctac accgcttcca
     6361 tggccggagg tatatatttc cttgcagcag ggattggttg atgcgcagga aaatgctaca
     6421 gacacttgtg tgggagcaaa cctccaggaa gtacaggatt atctgattct gacccaccat
     6481 atactgtatt gtaatcagtt cttaatcaat gccagcaaat ttgacagcct tgaccccttg
     6541 taccaagaag cactgcaaca agctattgat gaagctgctg tagaaattga agctcaatta
     6601 accgaaatag acgtaacaaa cagacaaaca ttagtagacg gcggtatgga attagtagaa
     6661 tttgatgcaa gtttcataga ccaagttcta gaaaaggcaa agggtgtata tgaatctata
     6721 ggcaatgata tcggacagga atatgtggat tctttgctaa atgcacttga aaaataaaaa
     6781 acttatctga ctaagcaaat aaactgaaca ggtagattaa gaccgccggc aacagccggc
     6841 ggttttagta ccctctattc actacaaaaa tttaatttta ttgaatgaat ataactttga
     6901 taaaatagga atcttcttca tttagacagt gaaattttca ataataaaat ctcttgtttt
     6961 aattgcggca ggtgataaat atttatcatc aatccagcac aggtagattt ctctttttaa
     7021 gggcatatta caaatcttta ccgtgtttac atgtttgttc atggatggta tttccggtac
     7081 aatcgaaatt ccgaaattaa gagaaacaaa tgaaatgaca gcattgcatt cttcagcttc
     7141 aaaaactatt ttaggagaaa aattataccg tgcaaatatt tcatcaacag tgctcctaag
     7201 agagctgctt ttatttaaag caacaaacgg ttcgtcgatt atatcagaaa tttcaatgca
     7261 ttttctttga gaaaagggat gctttttcgg cacaatcaaa tgaagctgtt gactgaagat
     7321 tttcacgcat ttaaggcttt tatcaggtgt cgggcaaaat gccagttgga ttttattgct
     7381 ttttaagtcc cttataagct gttcacccaa attctggacc aatgaaaact ttattaagcg
     7441 gtttgaattg ttttcataga attttttaat catctcaggg ataaattttg aacttatact
     7501 ataaatatat ccaagagaca cctctcccat tgaagaatcg gcaacggttt ttagcattat
     7561 agtgccttca tccaatatat ccaatgattt ctcaacatat ttaagaaaca gctcaccggg
     7621 attacttagt tttatctttt tcccatgtct ttcaaacaac ttaatattta attcgctttc
     7681 taattgagaa attgaataac ttaagcttgg ttgggcaata tgaagtattc cggcagcttt
     7741 cgtataattc tctacttttg ctaaaacacg aaaataatgc aattgttgta atgtcatctc
     7801 aaccaaacct cctaatatca aaataagcgg taagatgtta atactaagtg cggcttaata
     7861 attatagcta ttataccaga ttatttatag aatgtctcta taattttaaa aaattttgtc
     7921 attgctgtga tactatcatc atgaaccgaa gggtaagaac ctcaaggatg acagattgac
     7981 agggtttgag cagtgacaag ttgagggtaa tgtaggtaaa tcttagtgta aaacaaaata
     8041 ttgaatgatt tttattttta taatataata tttaaaacgt ttgcattaag atgatacatc
     8101 ttgtctttgt ctcaaataga tatacggtta ttgaataaca gggtggcaga atttcaaagt
     8161 tcagaaaatt ttgaatttct atggaaagca ggaatgaaat ggaaagtgtg aacaaataca
     8221 aatatatggg tttcatattg cttcaaagtg taatttatgg gtttggaaat ccacttacta
     8281 aaattgcttt taaaagcatt actccctttt ggagtctttc agttcgattc acaattgctt
     8341 ttatagtttt catgttattt tttggaaggc gtataattgg ctctttaaaa agcgtagagc
     8401 caaaggttta tctacctgcc ggcatatgta tggcaacagc ttacattacg tgtaatcttg
     8461 cattgagctg gacctcagcg acaaatgtcg gatttttaat gagtctgcct gtaatatttg
     8521 caccaatact tgcactcttg atatataagg ttaaatacag gctgatacac atacctgtac
     8581 aaattgtagt cgttatcgga ttatatcttc tctgctttga tggagaaaaa ttagtttttg
     8641 gaaaaggaga gattaccgca ttaatcacag ccatatccat agcaggagca ctggtatttg
     8701 gagaagaatc tcttaagcag ctggatgccg tagccgtatc tgctgtccaa gccggcataa
     8761 ctgctttaat aagcctgccc tgcgcattga tttttgaaaa tactgaaatt ttagtccatg
     8821 ttactatacc agcctggtta gtggttttgt atctggcgat attttgtacc aatattgcct
     8881 acatgcttca aaattcagct ctaaaataca tatcagcatc aactgtgtca atgcttcaat
     8941 gcacacagcc cattctaacg gcaataattt cttatttgct tcttagagaa aagcttagca
     9001 cattaggaat tataggagct gcaataataa tcttatgtat aattgcagaa aatataatta
     9061 ctacaaaaga acagtctgct tccaaaaatc ttgtctaaaa aaatagtaat tttctaatcc
     9121 ctttctgtat aaacataaat aggggggata tttatgttta ttaaaaaaaa tcagttttat
     9181 attatcattg cagttatagt ttttttaatg gctgtatata tgatttattc cattaggaag
     9241 gacaatcgtt taaacaataa cgaagaaaat gtaattaaat tggtagagga ttttggaagc
     9301 actctgaaga atgtgcctct tttggcacca agagatgaag ttatacaagc aattaaagat
     9361 aattatagtc catatgttac ggatgagctt atgcaaaact ggattgctca tccaaggagg
     9421 gctccgggaa gaactacatc gagcccatgg cctgacagga ttgaaatcaa tgaaattgaa
     9481 aggctgtctg aggatgaata ttcaataaaa ggaagaattg ttgaagttac aagcactgag
     9541 gctgaaggcg gtggggctgc tgctttcaga cctgttgaat tgattttaaa aagggcagga
     9601 aacaagtggc taatagataa aataataata aaaaattatg aatgacaaac agaaaatctt
     9661 ggaaaaatca ttatgtttgt ccgactaata gtaaacaaaa ttagacaagc gtaagaaaaa
     9721 ggtaaaaaat aataaaatat tttttctaaa ggtagttgac aaatgttaga aacaatgtta
     9781 tattacattt aaggattagc actcaacgag tttaagtgct aacaacaata gagataaagg
     9841 agtgttgaac atggctggat tagtacctta taacagaagg aacagaggtt tggttgacag
     9901 aggtttcagc gattttaggg acttttacaa catgcttgat gactttttct acgatgcctg
     9961 gccatctaaa aaatactttg caagagatac atttaaaatc gacgtacagg aaaatgacaa
    10021 tgaatactta gtagaagcag aacttcccgg tgttaacagg gatgaagtca atattgaaat
    10081 gaacgaagga agactgaata tttcaataca aagagaagaa agcatcaatg aagaaaacaa
    10141 aaattacgtt cataaggaaa gacgtttcag ctctatgagt agaagtctat atttagatga
    10201 tgcaaagtct accggcatta aagcaaaact tgaaaacggg gtattgagca ttattgttcc
    10261 taaggaaaac aaaccaaaca aatcagtaaa aattgatatt gaataatcaa acttaaagca
    10321 tgtggtcata atttccacat gcttttttta ttgaaaaagt atgttattat tagtagtggt
    10381 tcttagctgt aattgctatg aataacccgc agggtgtcat tctgaacgca gtgaagaatc
    10441 tcatgagatc cttcgcatac gctcaggatg acaaccggat tgtaaacaat taataaaatt
    10501 gaacaaaatc atgcagtatt tcgtcaatat aaaaaacaaa aaaaggagta attatgtctg
    10561 aagttttaag attagagcat gtaagtaaaa gtttcggaag caaggatgtt ttaaaagaca
    10621 taaatttcag tgtgaatagc ggtgaaatta tcggatatat aggttctaac ggtgccggca
    10681 agactacaac aatcaaaata attttaggtc ttatagacga ttatcaaggg gatgttttta
    10741 tttttggaga aaaagtaaaa ggacagtttg aatataaaaa aagaataggc tatgtacctg
    10801 aagtatctga tatgtatgat aatttaaccg catatgaata tttgagcttt ataggaatgt
    10861 tatacggtat aggggaagat aaggctgtaa aaaaaggaaa agaaatgatg agtgtctttg
    10921 gtatagacga tgccattaac ggcagaattc atacattttc caaaggaatg agacaaaagc
    10981 tgtcaattat caccggaatg attcataatc ctgatatttt atttttggat gaacccttgg
    11041 gaggaataga tgccaacagt gtattggttt ttaaagaagt attgaaaaat ttaaaaaaag
    11101 aaggtaaaac aattttttat tcttctcaca ttttggaagt tgttgagaaa ctaagcgaca
    11161 gaatactcct tataaatgaa ggggaaatag ttatagacgg aacagtggca gatgtaatga
    11221 gcatgcagtc ggattcatca ttagagtcaa tatttaatta tgttaccgga tttcatgatt
    11281 atgaaatgct tgcaaaaaaa tatacggatg tatttatgag ctgacggggt ctgatatgaa
    11341 tgaattttta tctttaaaaa tacttgattc tttcaaaaga atatttgaag gcttttgcat
    11401 agattataaa ttgcttcgaa ttatattgaa aaccaaatta atattggatt caaggcgggc
    11461 accggtgata aatacaaacc aaaagaagaa agatgaagaa aaggtttctc ttttgaaggg
    11521 atatttcatg catctgcttt ttggggcatt tttggcattc tttatttatt ttataaaaga
    11581 tgaaatgata cgcatgatgt atttttccgg tgcatttttc tttatgacta ccatgtattt
    11641 gatttctgat ttttcctttg tcttattgga tacaaaggat aaaaatatat tgttgacaaa
    11701 gccggtgaac tcaaggaccg tgtctttagc aaaagtattg catatactta tatacatgtt
    11761 caggttaaat acctttattg caggtccgtc tcttatcgca ttggtaatga gcaacgattt
    11821 atttgttatt ccgatatttc tggtggaaat attgcttatg gatttactga tatttttagt
    11881 gactgcattt atatatttat tggttttaag atttttcgac ggagaacttt taagaaatat
    11941 aatcaatttt attcaaattc ttttgacaat cttggttacc gtaggttatc aaattgccat
    12001 aagaatgata gattttactt cacttcgaaa tatggagtat acttttaatt caagacattt
    12061 tctgaatcct attttatggt tcggagcacc ctttgaaata ttctaccacg gaggagaatc
    12121 atacttatat gtttttacgg ctttaataat tattgttccc cttgtttctt ttatagttta
    12181 tttaagcctc agtaaaaaaa tggagagcct gcttcttaag ttagaagggg aaggtaagga
    12241 aagaccttca atacattgga ttgaaaaaat agtaggattc ataataaacc ctaatgaaat
    12301 ttcaaagcaa tcatttaatt ttgccaatgc catgtttaga agtgacagaa gcttaaagct
    12361 gaaaatttat cctgccttat cttccggaat acttcttccc atattaatga tatttaatat
    12421 gaggatggga ggaagggaat ttaagccttc cggttatgaa tatctttatt tgtattttaa
    12481 tttgcttata ttctcatccg tgttttattt agtgaaatat tcgtctgcat ggaaaggttc
    12541 atatgtattt atttcatcgg gatttaaaga ttataaaaat ttatacagag gattaatgcg
    12601 ctcaatggcc tataggtttt ttataccctt aattatttta aatggagtag tatttacatt
    12661 aatttttaaa tttagatttg tagaggatat agtaatttta tttttggcag gtattgcaat
    12721 tttgcctctt atcggtaaaa tatatttaga gattcccttt tctaaaccta ttgaggaatc
    12781 aaatcaaagg gattcttttg ataaatttct gctttcattt gctatagttg caatttttgc
    12841 tattgtgcat ttttactcaa atattattcc tatgggaaag tggatctatt tatcggtact
    12901 ggcagtcggg attccaataa gttggaaatt tgcaacaccg ggagaataaa tattattggg
    12961 agcatttatg gataaagtaa ctttatggac caggcaggat ataagaagtc tggatgattt
    13021 ggaaagaaag ggtgtaatca ggataacaag gaagcattta gaggagaaat tcgatgttat
    13081 ttccgaccat attatatttt tatataattg gtttgtcaat gaggcagaga aaagagtgcc
    13141 aagaccggag ggcgtggagt ttttcatctg gtgctccatt agcgaggagt atatgctaag
    13201 acctacatta aatgaaatag tatatgtttt ggaagtagat aaatctgagg tagtatattt
    13261 tgacggcatg aagtgggatt atgttctaaa tcatcactat gttccaaagg atgaaaagga
    13321 tgctgaagaa tatttaaaag acttagaaag aaagggtttt aagaattctt attcatttat
    13381 taacgaaaaa acagcccatt tttatccgca ggaaaggaaa agagtaatgg atagctgggc
    13441 tagagtgttt gaaattgatg aatgggatat tttcaggatt caagcaaata tatgggaaat
    13501 tcgccccgaa atgataaaag acatattaag agcagattct taaaatacaa aatctactgt
    13561 tacgttagcc tcaattctaa ttgtccccgg aattatggga gtagcagcaa attctctttg
    13621 aacaggaaga aaaggtattg ctgcattttc aacaatatta acaacaagag caggtgtaga
    13681 atacccaaga ttcattgtga ttgaattagc tttctgaatt gcatttaata tagccatatt
    13741 caaaacctgc tggtaatagt attccctgtt tgatatatca aaagttatca aatcaacaac
    13801 attggcacct gcgtttaccg cagcatcaat tatactgccg gcagcttcta aattggttgt
    13861 tcttatttgc aagatatttc taaccgaaaa acctctgtca atttgccttc catcttcaaa
    13921 atcatatact ctgtcaattg aatattgaga agtttttatg tcggtaattc ccattcgttg
    13981 aagtgtctga ataatagatt gacttattct tgcgttgtct gcctgtattt gagataagtc
    14041 ttctcccgta gtttgaaccc ccaaacgtat tataactatg tccggtgcta tttccatctc
    14101 gctatgacct gtcaatgtca tggttctgtt atgtacatta tccgatttat attttgtcat
    14161 cataatcccc cttttattta aaaattttcg aattatatta taatatgaac tttattaaat
    14221 tatgtgtcat ggtccatagg ggacggttct tgccggacat ttggagacaa tacgtcaggt
    14281 ggttactgtt atcacgttaa attataatca atttttaatg aaatagtata attattatgg
    14341 taaaataaat tagctaatat caaattggag gaattatgaa atttttgtgg actacaatac
    14401 ttgtaaataa tatggatgaa tcattaaagt tttatgaaga aatagtaggt cttaaaatga
    14461 ctgagagatt ccaggccgga cccgggatgg aaatatcttt tttgggtgaa ggagaaacta
    14521 aggtcgagct tatatacaat gagaaattta aaaagttgga tgcaggcaat agggtaacat
    14581 tgggttttaa agttgaatca ttggatgaaa agattaactt tattaaagaa aagggaataa
    14641 agataataac aggtcccata gaaccaaatc catcaatcaa atatttcata ataaaagacc
    14701 ctgatggagt aaaaattcaa tttgcccagc agctccaaag taattaagca aggtgttcat
    14761 tttgataagc ttaataaaaa aggatttaga caaactaaaa aaaagaagag aagattttaa
    14821 ataataaaaa ggtttaaaat cagcaagtac ggctcaatta aatacaagaa aagatatctg
    14881 attaacatgt aataattctg atcaaaagca aggttacgag tggggatatt atgttaagaa
    14941 tagcaatatg cgatgatgat aaggaactat gcattgagtt aaaaagcatg ctgaatgaaa
    15001 tcagcaaaaa tacgggcgaa gcctttgaaa tttcggtttt ttgtaacgga gaaggactgt
    15061 gcgattcatt gatgagcgga aatcgcttca acttgatttt tcttgatata gaattgtgtg
    15121 aaattaacgg tgttgaagta ggaaagaaaa tcagggaaga attaaatgac gaaatcactc
    15181 aaatagtcta tatatcagcc aaggaaagct atgctatgga gctgttcgat atacgaccct
    15241 taaattttct tgttaaaccg ttaaagatcg aaaaaattaa atacgttctt aaaacagcaa
    15301 ggaaagtatt aggcagcagc aatcaatatt ttgagtatag agtaggcaat gtaaatttta
    15361 atgtattaat aagtgatata ttgtattttg aaagcagcgg cagaaaagta aaggttgtat
    15421 tgaagaatga agtaaacgaa tattacggaa aactttctga agttgaggaa aagattaaaa
    15481 atgacgattt ttttttcatt cataaatctt atctaataaa ttacaatcat gttattgaat
    15541 attcctatga atatgtgaaa atgtccaata atgaaatact tggaataagt caaaacaaca
    15601 gaaaggcagt tcgtgaaaaa ttgctgcgaa aaaagcagag gatgcgccat gtcaaatagc
    15661 tatgttgctg tttacctgat aacaaatatg tttgcaacat atactcttta cagatatatg
    15721 tgcatatttt tcgacaggaa ggatgttgac aaaaggcagg aatttttgtc ttattcatta
    15781 tatttttgca taaacagttt cttgtacctg acctttaata aacctgccct caacattgct
    15841 tcaaatttaa taatgttttt tttgcttaca tataattatc agtcaacaac aaaaaccagg
    15901 ttgatagctg ttgtgttaat atatatgatt ttaatgacta ttgaagttct tgtgatgctg
    15961 atacttcaaa attacggtat aaccgtaaca tccggaaatg atgcggcttt aattacaggg
    16021 cttatcagta taaaaattat ttcatatatt tttatgttgt ttatgtcaaa ttttaaaatg
    16081 ataaataacg aaatcaaagt gtctaatatt cactggctgt ccatttttat tataccggtg
    16141 ggtaccttga tattagtgct gattatgatt tctatggatt atgcttccaa tgttaccgga
    16201 ataataataa gcataatgat tttgctgatt attaatgtgt ttgtttatta cttttacgat
    16261 gaacttatga agtcatatca atcgaaaatt gaaaaaaccc ttcttcagca gcaaaataat
    16321 gcttatttaa agcagcttga aattattaat caatccaaag aaaacattaa aacattcagg
    16381 cataatgtta aaaatcatgc tttaacatta aagtattata tagacaatca tgacagggat
    16441 ggcgccataa aatatttgga caatatattc gaatttatta attattcaaa ggagcatgcc
    16501 aaatcaggca attttgaaat agacagcctt ataaattata agatggattt ggctgagaaa
    16561 aacaatataa aagcagaagt gtatctggca attccccata agctaaatat aaatcccttt
    16621 gatttaaata ttgttatagg aaatttgctg gataatgcaa ttgaagcagc attaaaggca
    16681 gaaaacaaat ttatcaatat atccatcgaa ttggacagaa atgttctata tataagtatt
    16741 tctaattcct atgacggcag actgaaactt ataaagaata aattggtaac aacaaaagac
    16801 aacgataatc acggcatagg attaagcagt gtacaaaatt cgatagaaaa gtataatggt
    16861 accatgaata ttcactatga cgagcgagtg ttttatgtgg atgtattaat gtacaattaa
    16921 tattctatgt cttttcatat ttgatgaaca aatattaggg cttagtttca tgtttaagtt
    16981 ttgacataaa ttcatattaa ttattacatg gtaagaaatt aggtatgtat ttgtgataca
    17041 atggcattac attatagagg aggacgatgc catgaatttt gaagaatata ttagaaaagt
    17101 atatacaatt gcatttagac ttacaggaga cgaaacttca gccaaagacg ttgcttctct
    17161 tgctattgat aaaaatgcaa atattgtaaa agaaaatatt gattcaaata tattacatat
    17221 taccataaaa gaagtttgtg gcatatttct taaggagtat agtatattca gcagtgaatc
    17281 attttacaat aaaaataata caactataca aaacaaattg atgaatttag agccgctgag
    17341 caggacagca gtggtatgga aggacataat gggatataat atgaaggatt tggaaacaat
    17401 atcaaattgc ccaaaatcag agctttatga gaagttaaac aaggccagga aaaatctcat
    17461 ccacttttag tcatccttcg cgcagctaat gatctcaaac gtttccatta taccatctta
    17521 agctgcagcg aaggatctca tttggaggac taatgaaata taaatgtcta atacttgacc
    17581 atgatgatac agccgtaaaa agcacaccaa atatacatta tccctccttt gtggaggcgt
    17641 taaaatcatt aagacctgat attgaactaa gccttaatga ttttgttaat tattgtttta
    17701 atcccggatt ttcattgctt tgcaaggata tattgaaatt cagcgataca gagcagcagt
    17761 atcaatacaa gatttggaag gattatgtca ataataattc tccggaattt tattcaggtt
    17821 ttcctgaact aataaaggaa tataaaagct taggcggaac aatatgtgtt gtatcacatt
    17881 ctgaaagcca gcatataatc agagactata ctaagcattg tggtcttaaa ccggatttaa
    17941 tttttgggtg ggaacttgat gagcatcaaa ggaaacctaa ttcttatcca ataattgaaa
    18001 ctattaaaag gtttaattta agaaatacgg aaatgctggt gttggatgat tcaaaattgg
    18061 gtcttgacat ggcaagaagc tgtaatgttg aatttgcagc agcgggatgg tctcactcag
    18121 ttcccggaat agaaaaatac atgagagaaa actcagatta ttatttttca acaattgatg
    18181 aatttagaaa attcatattg gactgcggga acctatatga tttcaaatag ttaaactcat
    18241 acccggcatc ttttaatttc caattttttg ttgataggca aaaccttatt tgatatactg
    18301 gttatatcaa tatttttttg tgcaaatgga aaggtggaaa taattatgtc ggttgaaaaa
    18361 ttattacttt caaaccttag caccatagaa gcagaaattg ttatcgcaaa attaaaatca
    18421 tatggaatac cggtattaaa aaaatcaaaa ggcaccggtg agctaatgga aatatacaca
    18481 ggtgcaaaca tgtatggaat agatttatac gtaccttcag acatgattga attggcagaa
    18541 gaactgctaa aaccggaaaa tgaaacggaa tagttgcgtt atctaatttg actataggtt
    18601 gattacaaaa aaaattacta ataaaaaagg aatgaaaaga caatgatgtt taaaacatgg
    18661 atatggttat taataatagc tgttttatgt atattgctta tatacgtagc aaaaaagaaa
    18721 accaaatggt ttggtttgat gctttccata ctattctttg ttgtttcaat ttttatagtt
    18781 gtatattcaa aaagcatgac atcatcatat ttaatattat tcagtatttt tataatagct
    18841 aatattccaa cagcaataca attatatatt ttttttaaat atcccgaaaa gtagaaatag
    18901 ggacggtttt acgaaccgtc cctaataatc ttgatctcag ttgattatta atatagtata
    18961 atggagaaat ctgaagatat actaaggata aaaatgttac taaagaggag atattattga
    19021 agaaatataa agtatggata cttctgacat catgtgtttt gttttggtca ggtaattatg
    19081 ttttcggctc attggttgtc accgaattga cgccggttgg tctgacattt ataagatggc
    19141 tgtttgcatc ggttatttta ttgatagttg ggcaaaaaat agaaaagcct gattggaagg
    19201 aggtttgcaa acagtggcct aaaattacaa tgctgtctat gaccggcatt ataggttata
    19261 atataatttt gtactatgct ctatattaca catcgccgct taatgccgcc ttggttaatt
    19321 ctataaatcc aagttttata gcaatagttt cagcatttta tttaaaagaa agaatcacaa
    19381 aatatcacgt ttttggaata atgatttcat tcttgggtgt atggattgta ttaaccaagg
    19441 gaaatttgct ccaagctttc aatatgaagt ataacagagg agatttgctt gtaatagcag
    19501 ctgtgattac atggacatca tattctgtaa taagtaagaa gcttacaact gttcccccca
    19561 ttacttctac aggtgtttcg ggaattatgg ctggaattat actatttccg tttgcaatga
    19621 ccagcggagt taattattta aatatgagta ccttggcatt gacagccgta gtttatatta
    19681 ttttgttccc gtcattatta tccttcgtct tctggaatat tggagtgagg gaaatcggtg
    19741 caggcaagac aggcatattt ttgaatttaa accctgtgtt tactgcattt atcagcaggg
    19801 caatggggaa ggatattaca tgggtgcagg ttataggggg actattagtg tttacgggag
    19861 tttatataac tactgcattt aagggtaaga ctgcaaggct caaatgagga ggttataatg
    19921 gagaaaggtt atattcatat atatacaggt aacggaaaag gaaaaacaac tgctgccttt
    19981 ggtcttgctg taagggctgc tttatccggc aaaaaagttt ttatagggca gtttgtgaaa
    20041 aacatggagt acaatgaaac aaagataaaa gattatttaa gcaatatcga aataaaacaa
    20101 ttgggaaggg gatgcttcat atacgaagat cctgaagaaa ttgacaaaga aacagcaaaa
    20161 aaaggcttgg aagaatgcag cactcttctg gcaagcggag aatatggact gataatcctt
    20221 gatgaaataa acatagccct ttattttaag ctgcttgaag tatctgatgt tgtaagtgca
    20281 ttaaaaaata aggccgagaa cactgaagtt gtgttgacag gtcgatatgc tcccaaggag
    20341 cttattgaaa tagccgattt agtaaccgag atggttgaaa taaagcacta ttacagccgc
    20401 ggagtattat ccagagacgg tatagatcac taaaattatt agggacggtt ctttttagta
    20461 agaaccgtcc cttaaaaatg aaatgtcatt tttttactgc ctttgttgca taaaaggtag
    20521 gcatattcaa ttcatggagc cttcctgaac cgtttgtatc ttcataaatg tcctctataa
    20581 taaaacctgc ttttatttgt ccccctattt gttcctcaat agtatgagaa aattgaatac
    20641 cccaatcctt ttctaccgaa agctgataaa gctcttcatc cttcagcgga ttaaagggca
    20701 gcttgcgtac aggtgttgtt tcatcatcat cgaaaataaa attaattccg ttatctaatc
    20761 ctgccaataa tattcctcct tttttcaaca ctctgaagca ttccttccaa acaggaagaa
    20821 catcctctat atagcagtta gatacaggat gaaatatcaa atcaaaagtt tcatcctcaa
    20881 agggcaaggg ctttgtcata tcagccctta tagttttaat ctcataattt tcccttttag
    20941 caacctcaat ttcacttgca atttgtttct ctgaataatc aagtacagta cattttgcac
    21001 ccaatgcagt gaaaattggc atctgctgac cgccgcccga agcaagacca agaacttcgg
    21061 cattcttcat ctcgcaaaac cattcctttg gtacaggctt agtcggtgtt aacaaaacaa
    21121 accaatcatt gttttttgct ctttcaaata cttcgtgact gacaggctgc ccccattccc
    21181 atccttcatc aatccattta tcaatgaatt ttgaattaac ttctgtatac tttggagatt
    21241 ttggagacgg tttttttcct tcttcgggtt ttatctctga ttttttcata aaactatcct
    21301 ttcagtttta tatatcgttg atacaatatt atcatttata ttatgaaaat caatataatt
    21361 tttatatgac aaggggacgg gggtattgtc agctttttgt tttgagagcc cttagttaaa
    21421 aaaattccaa aaaaatatat tgacaaagtt gatatattgt atataatgga tatatacact
    21481 atttacggag atggaaatga atataataat aagcaattca agcggtcaac caatttacga
    21541 acagattgct tctcagataa agtctatgat tatcacaggt gaattaagag agggagaggc
    21601 actgccttcc atgagacttc ttgcaaagga gctgagaatc agtgttatta caacaaaacg
    21661 ggcatacgat gaacttgaaa gggaaggatt cattgtttcc ttcaccggga aaggaagttt
    21721 tgtggcaggc aaaaatatcg aattgataaa ggaacagcat cttaaggaga tagaaaacca
    21781 catgcaggaa atacttaaat tatcaaagga atgcggtttg aagctggatg aaatgattga
    21841 aatgatgaca ttgctttatg aaggagaata aaatggaata ctcattgaaa ctggaaaatg
    21901 taacaaaggt ctacaaggat tttaagttag acaatatcaa cattaccctg cccaagggaa
    21961 gtataatggg ttttataggg gaaaacggag ccggaaaaac caccaccata aaactgattt
    22021 tggatttaat cagccgggat gggggaaata tttcaatttt aggcaaagat aacaaaactg
    22081 aaatggattc cgttaaggaa aacatcggag tcgttttgga tgaatctagt ttccctgaaa
    22141 atctaaacgc aagagaaata aacaacatgt taaaaaaggt atataagacc tgggacgaag
    22201 gcaaattttc aaaattcctc gatatgttct cactccccta taataaaagc atcaaggatt
    22261 actcaagagg aatgaaaatg aagctgtcaa cggcagtttc catgtctcat gattcaaaaa
    22321 tattaatttt ggatgaagcc acgagcggcc ttgaccctgt tgtcagggaa gaaatcctgg
    22381 ataccttttt ggactttctt caggatgaaa caaattccat ctttgtatcg tcccacataa
    22441 taagtgattt ggaaaaaatc tgcgactaca tcacctttat tcacaagggg aaaattatat
    22501 tctcggaaac caaggacgat ttgctggaca attacggtat cttaaaatgc tctccggaag
    22561 agtatgaaaa cattgataag agccttgtaa agggaatgag gaaaaacaaa ttcggaatcg
    22621 aagctttggt gcttaaggat gggatcacgg gaccttatct gattgacagg gcttccctgg
    22681 aagatataat gatatttatt ataaaggagc aagtgcaatg actggattga ttttaaagga
    22741 tttgttgaac ttaaagaaac aagggaaggt gtacttaata ttaataatat tctattttgc
    22801 cataggtctg gtcaatgaaa actcatccat gttcgcctcc atgatgacta tggtggcagt
    22861 attgatacct attacggcaa tggcttatga tgaaaggtgc aaatgggaca gatatgccct
    22921 tacaatgccg gtatcaagaa aagacatggt tatgagcaag tacttgctgg gcttgatatt
    22981 tttgaccgct gcttttgtta tatcaattct tcttaacatg gttttttcca atatgcccat
    23041 aaatgaaaaa atcttgacag ccttgggcaa cttttttgcc ggattgacat tgatggcagt
    23101 catctttccc ctcctcttca agttcggagt ggaaaaaggg cgcatcctga tgatgattgt
    23161 tatattcata ccggtggcag ctgtgatgat gatttcaaaa attggaatcc caatgcccgc
    23221 agaggaaaaa ttagaacaat tgatttattt tttacccata attggtgtac tcttatttat
    23281 aatttcgatt tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttatgaa
    23401 tttgatgcag tgattaaaaa ggttccggat atagacgggg cctatattga gtttccctat
    23461 gatgtaaagg aggaattcgg aaaagggaga gttaaagtat atgcaacctt tgacggtgag
    23521 ccctatgtag gaagcttagt caggatggga actccttgtc atataatagg catcagaaaa
    23581 gatatacggg caaaaatcaa taaacaaccg ggggacatag tccatgtgac aattagagaa
    23641 agaagttaat agttgcgtaa tctatagaat aacaattatc aaaaatagcc gtacttgtgg
    23701 caatggacag gtcgttttaa gcttttgaga ataaaaggaa ctatccctaa aattttggaa
    23761 ctatttgtta ataggtaacg tcttattgat ataatacaat aattagcaaa aaccggtata
    23821 ggaggtttaa atggatttta aaaagtttat ggttttattt ttgtgcatat tattatttgc
    23881 ttcgtgctca gataataatg tagttaagga accggaaccg gtagaagagc ctgaagttat
    23941 tgaggaagac aatataccta cagcttggaa cttgagtatg gaagaatttc gtgtagacgt
    24001 tccttttagt gttcctgatg ttatacctgt tgtggagaaa tatgaagtca atgaagattt
    24061 atctaacctg gtaaatgcag gccagtatgc cggatttaca gataagcagc taaaatcaat
    24121 ctatgaagat ggttttgtcg tattgaaacc aagctatgaa tatttaaaaa tgcatcattt
    24181 atatgaatac cccatgtata aggaatcacc tgtatttata acagttgact ccgctctcca
    24241 tttataccac attttttacg gcaattcact aaagctcctt gaagtatctt cactgtatga
    24301 taaattgcag tccctttcaa aaaatatgct gatcgaaagc ttaaatgcct ataatgattc
    24361 caagtatgct aatttaaagg aagaattgaa atttgcagcc gcatattttt taaccggagc
    24421 aaaattgatt gatgaagatt tggaaggtat agtagtcccc gaggaaattg cagttttatc
    24481 cgacgatgag ataaagctta ttgatgaagc ctttgatttc gcaaggtctc ccatcttcgg
    24541 aaaggatttg gattattccc aatttaccgt aagaggccac tatacgggaa atgaagaatt
    24601 aggtaaatat tttaaaacta tgatgtggta cggcttaagc ggattcccga tatttgatga
    24661 aagcaaaagc aagccggtcc ttgatatgga cagcttgaca aaatcaatga taattacctg
    24721 tcttcttctt agaaatgaag atagttttga tgatttcgaa aatatttata ctgcaacagc
    24781 tctttatacc ggcatgtcag acgatttggg aattttcgaa ataagagact taataacaaa
    24841 ggtatacgga cagaatcctg atttgaacaa atttaaggat aattcatact atgataagct
    24901 tttaggggaa gcacttcttt tgcctgaacc aaaaattcag cctaaatatt cttcagtcag
    24961 tacacctgcg ggaaggcagt tcaggcttat gggacaaaga tattcctttg atgcggaagt
    25021 aatgcaggca ctaatcgagc caataataag accgattcct tcaggtcttg atgtgattgc
    25081 atcctttggc agcaaaagag ccgaagagct tttggataca tactacaaac caaaggaaga
    25141 ttgggataaa tacgaagaaa atttaaacct catgcgaaaa aaacagactg aaattacaga
    25201 tgatgaatgg aaatcagatt tatataaagg atggctgtgg agcattaaat catctgccgt
    25261 atccttcgag gataaagaag gtatgcccca tttcatgaga aatgaaaaat ggacggacaa
    25321 aaacattcat acagctcttg gcagctatgc cgaactaaag catgacagca tcttgtacat
    25381 gaagcagtcc ggtgccgaaa tgggcggagg acctgagcca ataatacctt ataattatgt
    25441 ggagccgaat gtggaagtat atgcaaagct taagtggctt gcagaaaata cgaaagcaca
    25501 gctacaagaa agaaatatgc ttaaggatga aatcggtctg gtattagacc aaataattga
    25561 tattcaagat actttaatga atgtttcagt taaagagcta acaaatcaag atattacaga
    25621 tgaagagaat ttaaaacttt acagatacgg tggattgatt gacagtgtaa ttcaaataat
    25681 gcaaatgaat cttatgagga atgacgttga tacaagcaat gatttcacta cggcattaat
    25741 agcagatgta tcaacaattg cgccaaacga cctctttccg aaaggaacgt atttggagat
    25801 aggaaacggc ctaccctgtg aaatctatgt agtttgtcag accaacggaa aaacttattt
    25861 agcaagggga gccttgttta actattatga atttctttcc gacaagagac ttactaatca
    25921 cgaatggcag accttggtgg gggtaaagag aatggctatg gtttatgacg aggaaaagaa
    25981 tattcatgtc cccatggata tatacgacga agacggaaac aggattctcg aagaagatga
    26041 atatgatttt gagaatataa tgattatcgg tccttcagaa aacatggttc ctaagccggc
    26101 atggacagag tcatttatat cacaggaaga aaacaaggta accattaagg atataagtat
    26161 tagctgggag taaaatgaag agactattaa taccgctagc ggtcataatg gcaggggctg
    26221 tctgtgtact cattagacag cctgaatctt atgccgccgg aaaaatatcg atagaacaga
    26281 atacctatgg tttatatgaa aaaagcaatt atttatatat aaaaagtggc aagaaatatg
    26341 ttaaaatcat tgatgaagaa ataattgatt ttaaaacata tgacattgac tctgacaaag
    26401 atgatgagat tttggctata tctaaacata tttctgaaga atacggcagt gatttcatca
    26461 tatatgacat atatgaagct gacggtatca tattgacaaa agaaatttac agacaggatt
    26521 ttttcgattt caaaccttgg aaggttgatg cctgtaattt ggataatgac ggtgaaacgg
    26581 atatatttat cggggtatat aaagacactg ttttctataa ggatctaaga aagaggccat
    26641 tcttttattc ttgggacgga gaccgcttgc ataagaagtg gcttggctca tttttctccg
    26701 actgggattt ggtggacata gcttttggcg atttttataa tttaggctat gatgtggctg
    26761 cggttttgga aaaaaacaag gatgcctgta gaataggcat ttaccaattc ataggatttg
    26821 gctttgaaaa catgaagact ataaattccg tggacaattc ttttcgttct caaacagagc
    26881 tgctgtttgg caaatagagg gttattaatt aatttttaat agttctttaa ggattacata
    26941 attttatgaa attataatag atactaaaat taagtcgatc tgcaatttgg agggatgatg
    27001 aaaaggtttt ttacacgtaa gaaggttatc gtttttgttg tggttgcggc tcttgctgca
    27061 atcatatttt ggcttgtaaa caagggttcc aaggcgccgg aagtgacggc aaatgtttca
    27121 aaattggaaa gaaaaacctt agagcatgtt atttcggtga aagcacccct ggtagggatt
    27181 gaaaaggcgg aggttacatc tcctttaaat tacaaaataa acgatataag ggtgaaggaa
    27241 ggagatttgg ttaaaaagga cgatatttta gccgttttgg acagcgagga cctggaaaaa
    27301 gaaatagaat atgaagaaaa tcaaattgaa ttaagcaaaa tggaagcaga ggagaaaatc
    27361 aaggccctgc agattgaata cgacaaggct ttggcaagct acagggatat ggaaacagta
    27421 tatgagcaaa acaaggacct gtacgaaaac ggcgtaataa ccgaagaagc ctttaaaaag
    27481 acagaaaagg atctggttgt tttaaagaaa agcctagaag gttataatgc cgtcaatggg
    27541 aaaatacaat tgtcccaagc agataaaaag cgcattgaaa tacaggaaga ccggcttaag
    27601 ttaaagaaag aagaacttga aaaggtcatg ataagaagtt ctattgacgg gaccgtcacg
    27661 agggtaaatg tcaatatcgg aagatatgcc aaggataccg aaaacagcca ggcaatgttt
    27721 gtcgtggaaa atctggaaaa gctgcaaatg aaggcagcag tcagcgaatt tgacgtggga
    27781 aaacttaaat tgaatcagga agtagaaatt tattctgata ttttgggtaa gggatttgca
    27841 aaaggaaaag ttgcgagaat atctcctaca gcagagcaaa aagataataa caccatggaa
    27901 agagtgattc ctgttttaat agatgttacg gaaaggccgg aaaatttaat agcaggagta
    27961 attgccacag ctaaaataag aaccgaaaag aaagaaaatg tatttgcggt cccatcggga
    28021 gccatagtgc aagacgggga ggaatacaag atttacatat tgaatgaaga caataccgta
    28081 aggtccatac cggttgaaat tggtcttgaa accgatttgg aatcagaaat cacgggtgat
    28141 ttatacgagg aaatgaaata tgtgataaat cccgacgaga cattcgaaga cggtatgaca
    28201 gtaataccta atgaaattga gtaggttgat atggaaaaga tgattaggat agaaaacttg
    28261 aataaaatct acaaaaacgg ctccgtggaa gttcatgcct taaatgatgt gaatatggag
    28321 gtctcccggg gagaattcct ggctgtcatg gggcattccg gctcaggaaa gtcaaccctc
    28381 atgaatatta taggttgtct tgacagacca agcagcggca agtattttct tgatggaatt
    28441 gaaattgata aacaaagccc cgatgagctt tctttgataa ggaataaaaa aatcggtttt
    28501 gtttttcagg catttaattt aatccccagg accaatgtgc ttagcaatgt ggaacttcca
    28561 atgatatatg ccaaagtcaa gtcaaaacaa agaagtgaaa gggctttgga gcttttagag
    28621 aaggtaggct tgaaggatag gataaaccat atgccaaatg aattatcagg cggtcaaatg
    28681 caaagagctg ccatagccag agccttggca aacaatcctc caataattct tgccgatgaa
    28741 cctacgggga atttagacac caactcttct gaagaaatca tgaaaatatt caaagacctc
    28801 aacagggaag gaaccagcat catagtggtc acccatgaac ctgaagtggc aaaatatgca
    28861 gacagaatta ttgtgtttaa ggacggatgc attatcgaag acagatggaa agagggtgga
    28921 agaaattatt tggcttgaaa atattaagat agcttttaaa tctattttat caaacaaaat
    28981 gcggtctctt ctcaccatgc tgggaattat cataggaata agctcggtta tttcaatcgt
    29041 gtccataggc gacagcatga agggggtaat ggacgatata tacaagaatt tcggcaaaaa
    29101 cagggctgtt ttgtatgtgg gaaacataga ggatttcaga accacggact ttttttataa
    29161 cgatgattta gaaattttaa aggaaagatt cagtgaaaag ttggcttata tttgcccttc
    29221 cgattccttg agaagcgatg ttacggcagg caggaatacc ataaaactca atatggaatt
    29281 cattgactcc ggatacgata aagtccaaca aattaagatg ctttacggca ggatgataaa
    29341 tagcaaggat atagaaagaa agagcaaggt agttgttttg gagcaaaaag ccgccttaaa
    29401 gctcttcggg aaggacaatg ctgtaggaaa aacaattagg gttaaaatcg acaacaatat
    29461 ggaagacctc ctggttgtgg gagtatataa aatagagcaa tcccctcttt taaccttgat
    29521 gcagggacaa agcaattatg aaaacagctt cataccttat actatgccta cttcatcata
    29581 ttacgcctat tatgccttgg atatttttat tgatgaaaag tattccatgg ccattacggg
    29641 tgatgagata ataaattata ttgccagaat gaaaaacagg acaccggaaa actacatata
    29701 ttataccgtt gaggaagaac aaacacaggt ggaaagtgtt gtgaccggct tatcagttgc
    29761 ggttgcagcc atcgcagcca tatccctcct ggtgggggga ataggcataa tgaatatcat
    29821 gctggtatcc gttactgaaa gaacgagaga aataggaata aaaaaagctc tcggcgcaag
    29881 aacaaaagat gttttgtttc agtttttgat agaatcagcc acactttcag caatcggggg
    29941 gatagtggga accataacgg gcataggttt agttatgata gggggttctt ttatatccat
    30001 acccgtggtt gtaaatccgg cttctatcat tttggctgtt gtatttgcca tggtaatcgg
    30061 gatattcttc ggctattatc cagccaagaa ggcagccaat tcagacccca tcgaagcttt
    30121 gagatacgaa taataacaaa aaatacaaaa aaggagtgat attgtgatat tgtcggaaga
    30181 acaaaaaaat cattataaaa gacaaataat aatacctgaa ataggtgaac agggtcaaaa
    30241 aaagcttctt gattcaactg tcttggtgta ttgcgaaaat acagacagtc taagaccctt
    30301 ggcatactat cttgctgcta tgggaatagg aaaaatattt tgtgatttac aagataaaat
    30361 cggcgctgac tccttatttg ctgaggtaat tgatttaaac aatgatacaa aaattgatta
    30421 tcttaatgaa gaaaactatt cgggaggttt ttcggagata tatgaaaata acaagaacca
    30481 tccccaaaat ttttgcagga ttgttttggg aaattatagt tttataaaaa attcagcagc
    30541 taaattatta aacgataaat atttaccgac tattatatca gctaacagcc aatggaaggg
    30601 tacactgcag acatttatca atgaggattt gtttaagaat tttattgcat taatagcaga
    30661 taataataca aaagatttta gtccgccttt tttagccaat gcattatctg caacgctttc
    30721 ttctatagaa tgtgttaagc tttgccttaa tatcggtaat ataacaaaag atatgctgtt
    30781 ttttgatttg tttaatatgg aatttaaaca aacagataat aatgctaact tttttgaatt
    30841 tttaaatgaa gcaaataaaa tggatattaa acaaaaatta tctgatgcaa aagtcttgat
    30901 tgtaggtgtg ggaggcttag gctctccggc agcctacgga atggcaatgg ctgatgtcaa
    30961 cacattgggg ttattggatt tagatgaagt ggaaatgagc aatttaaaca gacaaatact
    31021 tcattccttt tcacgcatcg gtatgccgaa ggcaagctca gcagaattta tgctaaaaaa
    31081 gctaaaccca agcataaata ttaaaaccta taaaactgaa ttaacccgag ataatgcaga
    31141 aaaaattttt ttggaatacg acctgattat tgctgcggtt gataatatac cttcaaggta
    31201 tctgataaat gatatatgct ttttattaaa taaacctttt gctgaggcag gagttttgag
    31261 atttgacggc actgcaacca cccttgttcc taaggaagga cattgttaca ggtgtttata
    31321 tcccaatgtg gacagcagca atttatcagg taataatggt gtcttaggtt ctgttccggg
    31381 agttatgggt tttatacaag cggcagaagc agtaaaaata ttgtccggat ttggaaaaac
    31441 cttaaaaaat aaaatactct tgtttgattc attggcaatg gattttaata ttattgatat
    31501 tgaaaaaaat ccccgatgtc ctacatgtgg aaaataacct attattctca tttatgtaag
    31561 aaatttttta actgaaaaat ttctaaatat ttaaattttt acctctttat attgagttat
    31621 ttctggagat actattaaaa aataattcat tatggaggtg actatgaaaa aatttaaaaa
    31681 atttacaatt attttttgtg cattaatgct tttattggtt gcatgtgcac ctggagatgg
    31741 aaacctaaat gaggacgaag acaacgagcc tattgatgaa cctattgatg acaataatac
    31801 cggagcaaat gcaaataatg atacctttga cgaagaagaa aaaacattga caggatctgc
    31861 acaaggatat ggaggagaaa taaacgtaac agttgtagtt aacggagagg atatagtatc
    31921 ggttgaagct gaaggaaaag atgaaactga gggtgtaggc tctttggcaa tagatgaact
    31981 tcctgataaa atagcacaag caaattcaac agatgttgac aatgtatctg gtgctacgaa
    32041 aacaagcgaa ggaataaagc aagcggttaa caatgcatta cagagtaata aacaatcaaa
    32101 acaatagcaa aatgcatatg aaaaaccagc cgacaggctg gttttaatca tataaggagg
    32161 ataaaatcaa tgacgtggat gattccctga tacattcgat tttatgcagc tcgtcttgaa
    32221 tagcttttaa acctgaaggt gcactgcatc ttactttcat aagtatgcac caagtgccgg
    32281 tgactatatg gcattcttca attactaaat caggaatagc caagtttttt aattgttcca
    32341 aaagcttatc ataatcagca gttctcactc ttaatgaaat aattgcatat gtggtaaaac
    32401 caagttttga ccagtctatc attgccttgt atcctgttat aataccctga tcttccaatt
    32461 ttgcaactct gttatgtatt gcaggtcttg ataaatttaa tctttttgaa agctcttcgt
    32521 gtgaaattct cccttccttt tccaagtgtt taattatttg catgtctaag aaatccatat
    32581 ctacctcttg tcatatcgta aattatttga agaataaaaa actaatcagt tataatatag
    32641 cacaaaaagg tacaacatgt aaataattat ttaataaatt tttaaataat gc
//
LOCUS       DAOPUE010000010        29782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1493_length_29782_cov_11.455882, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000010 DAOPUE010000000
VERSION     DAOPUE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 29782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29782
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_29782_cov_11.455882"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..665
                     /gene="lepB"
                     /locus_tag="PKH42_01910"
     CDS             159..665
                     /gene="lepB"
                     /locus_tag="PKH42_01910"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOA19213.1"
                     /translation="MKTFINEWVIPSIIAVVIVLFLNRFIFILVTVPTGSMETTIMPG
                     DRLYVNKLFDIKDAKRGDILVFQSDELDARLVKRLIGLPGETVEINATGEVFINGEKL
                     DEPYINNANGEERTFKIPEGTYFFLGDNRPVSVDARYWSNPFIPEDKIIGKVAFRFFP
                     FNRIGKVE"
     gene            963..2615
                     /gene="abc-f"
                     /locus_tag="PKH42_01915"
     CDS             963..2615
                     /gene="abc-f"
                     /locus_tag="PKH42_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HOA19214.1"
                     /translation="MLIIECSKIKKYYGDRLILDIDNLNIYSEDRIGVVGLNGAGKTT
                     LINILSQRIEPDKGWVKLRGKTEYFSQLEEPEVYEISSEMASKFGVETLWNENMSGGE
                     KTRFKLAKALENESILIFADEPTSNMDIEGIELMEKKLTEYKGALIIISHDRSFLDKL
                     CNKIIEIENGKIKTYEGNYSDYSRQRLHDKERAQFEYEEYIKEKERLGSVIADTKQKI
                     KSIKRPPKRMGNSEARLHKMGGQKAKANLERKVGNLEKRIEHLEVKEKPKKISKIKID
                     IMPSDYIHSKIIIEGKKVNKSFGNKIIFKDAEFCIYNGSKVALIGPNGCGKSTLVKMI
                     IDKDNSIRLSKGAKIGYFSQDMNILNNSLNIIDNVMESCNYSENFARLLLARLLFKGD
                     DIYKKIDVLSGGEQIKVSFAKILLQDINLLILDEPTNYLDINSLEVMEDTLKEYDRTL
                     LFVSHDRSLISKVADHIMTIENYKIKTFNGTFAEFKEIQSKKKLDKDVNMQIILLENR
                     LSDIVGRLSMQLKKEELEALDKEYYEVLEKLNKLKTLRTTPV"
     gene            complement(2641..3120)
                     /gene="rlmH"
                     /locus_tag="PKH42_01920"
     CDS             complement(2641..3120)
                     /gene="rlmH"
                     /locus_tag="PKH42_01920"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOA19215.1"
                     /translation="MKITIISVGKIKEKFFTEAIKEYIKRLSKYCKFIEEIVPDERAD
                     ESFSPAEIEQVKIKEGQKILNKIPKNSYVIVLDINGAQLSSEAFAEKLNTFGIEGISD
                     ITFIIGGSNGLWKEVIKSADFNLSFSKMTFPHQLFKVILLEQIYRAFKINSGETYHK"
     gene            3382..4743
                     /locus_tag="PKH42_01925"
     CDS             3382..4743
                     /locus_tag="PKH42_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19216.1"
                     /translation="MIKIKKIICLVICISMLGTQAFGFSQAYNEFDIKKSIENTYGIN
                     IIIPEGVDYSEFIDCMSILERSIKKFPDNIIKEITDYYLSKGIATNVILDKTEIIKEL
                     FTLSIIDESSVNIYIKTLEGSLYSESHYASEQVITHVFSHFISDYMLETYDLDNLKDE
                     FDKLNEGFEYGTWSEEYSKVFVNRHSAISFENDIAHLIWYAEANPFILRNINGGDYAI
                     IHEKLRLIAEEFSKIFNSITENTKLWLDAIPQTPQDWAKDTITEMKNQSLIPDEFNGL
                     YEAYIKRGDFYKLVLNMLHIKLGENELNKYFNLTDYEEHVALDPLRGEVYVTDGLNYT
                     SYYNYLANSSNAFYEAYQMGILNTDSFYEPDEYMTRLEIAKLLAYIANELGMNISEFQ
                     TVSFNDIEQVAENEKPYIYIAADKGFIKGDGLNFKPFNYCTYQEAYLILNRFYNSLYD
                     YIQ"
     gene            4764..5480
                     /locus_tag="PKH42_01930"
     CDS             4764..5480
                     /locus_tag="PKH42_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19217.1"
                     /translation="MFDLKSIVIGLVLMIKKYSCLSLTIYEEANEEDYKNGIIALPII
                     GLAIGLVAFIIVSFRYIYDGFFISSAVLLYYCVVTKTTNIKDVYRTLNYYIKPANQTD
                     NISGIIGTVLICFMYLSLFRVVPVTSLIIMPAAGFSNLMILSKVIKRDLENTSILKYC
                     GNYHIIAAFGISFLSAAILNYKLIISLSLMFMLSGLIVSILDKRIKNVPSSVEGFIIE
                     VSQIMFLIITYIIRFGDTPL"
     gene            5655..6653
                     /gene="trpS"
                     /locus_tag="PKH42_01935"
     CDS             5655..6653
                     /gene="trpS"
                     /locus_tag="PKH42_01935"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOA19218.1"
                     /translation="MENKNRIFSGMQPTGSLTLGNYLGALKNWVALQNEYDCYYCVVD
                     MHAITVPKEPKDLRKNTYEVLANYIAAGLNTEQVTLFIQSHVSAHAELTWILNCFSYY
                     GELGRMTQFKDKSKKQDSNAITAGLFTYPVLMAADILLYQADLVPVGEDQRQHLEITR
                     DIAQRFNNRFGETFVVPEPYISTDVAKIMSLQDPESKMSKSDSNENSYVLITDSPDVI
                     RRKFKRSVTDSIGVVNYTDEQPGVKNLINIYSKISNIKPEEIVSNYESKGYAKLKEDV
                     AEVVIEELKPIQERIDYLLKNKDYLEKIYNAGAQKAEVVANKTLKKAQKKVGFIPR"
     gene            6888..8711
                     /gene="typA"
                     /locus_tag="PKH42_01940"
     CDS             6888..8711
                     /gene="typA"
                     /locus_tag="PKH42_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOA19219.1"
                     /translation="MKNKIINIAVIAHVDAGKSTLVDAFLQQSGVFRENQEVSEQIMD
                     SNDLERERGITIYSKNCSVMHNDIKINIVDTPGHADFSSEVERIIKTVDTVILLVDSI
                     EGPMPQTRFVLKKSLEIGIKPILLINKIDKKNHRAEEVVDMVFDLFVELNANDEQLDF
                     PILYGIAKQGIVQYELDEESHNINPLFDTIINHVQPYPDRDDEDLQLQVSTLAYDDYI
                     GRLGIGRVTAGTIKEGQIVSVAKHDGSVEKQKISNIYVYEGLKRTQVKEAFSGDIVIV
                     SGIANISIGDTICNENKIIPMETIKIEEPTMSMNFLINTSPFAGLSGKYVTTRHLKAR
                     LEKELEVNVGLRVEALEGALDGYKVSGRGELHLSILLENMRREGYEVAVSRPEVILKK
                     IDGIVMEPIERVIVNVPEIYSGSVISKLNLRKGIMVSMVPDGNYVKIEYLVPTRGLLG
                     YRTELINDSRGEGTMVRVFDHYEKFKGEIPQRINGVLISNDQGEAVAYSLSNLESRGI
                     LFINPGDKVYEGMIIGMNSRGEDITVNPCKTKKQSNIRSSAADDAIKLSPPKIMTLEA
                     ALEFINDDELVEITPDAIRLRKKYLTEIERRKYANKIKTSN"
     gene            complement(8862..9341)
                     /locus_tag="PKH42_01945"
     CDS             complement(8862..9341)
                     /locus_tag="PKH42_01945"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOA19220.1"
                     /translation="MKKIESFQVNHLKLKRGIYVSRKDKVGENIVTTFDIRMKEPNRE
                     PVINTAELHTIEHLGATFLRNHPVYDTRTIYFGPMGCRTGFYVIFSGNLTSLDVYEII
                     KEMFLFIMNYEGDIPGADEISCGNYQDMNLNMAKYEAAKFYNEVLLNIKDENLNYPE"
     gene            9579..10973
                     /locus_tag="PKH42_01950"
     CDS             9579..10973
                     /locus_tag="PKH42_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA19221.1"
                     /translation="MKAKECLLLLQKILQLVDEGVHVLDKHGNTIIYNEAMSALEKMD
                     SADVIKKPFTEVFKGFNTENSTLLKALHSKTVTKNLKQTYLNKDGKEISTINTTIPVI
                     IDDEVVAAVEVAKNITKIEEMSNTILKLHDEIEKPEMVKHKKVKKYNFDNIIGRSENF
                     LDVIERAKKSSKNSASVFIYGETGTGKELIAQSIHYASARKDKPFKAQNCAALPESLL
                     EGLLFGTTKGGFTGAVDRPGLFEQANGGTLLLDEINSMPYELQPKLLRVLQENYIRRV
                     GGSTDIPIDVRIITTCNEPPEKILKEGKLRKDLFYRLNVIPINVPPLRERVDDIILLA
                     KMFVDKHNERFNKHVTGFSDEVYEYLLSYDYPGNARELENMIMSAVSMCEDDEYILTV
                     NHFSVHRSIEPRIDDYEKVEDSGIDEYLGNLEKRIIEKALLENNNNITRTSNALKIKR
                     QTLQHKIKKYKISV"
     gene            11219..12277
                     /gene="ord"
                     /locus_tag="PKH42_01955"
     CDS             11219..12277
                     /gene="ord"
                     /locus_tag="PKH42_01955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOA19222.1"
                     /translation="MQNVKVIIWGLGAMGGGMARMLLEKKGVDIVGAVGRGAKLGKSM
                     YEIIGVERGDRPEVLVGTPEDVIKEKAADVVLCCTDSFTKVAFERLKFCLERKINVVS
                     SAEEMAFPQAQNPELAKQLDEIAKANGVSIIGTGINPGLIMDYLVVVMTGCCQTIDHI
                     TARRVNSLSPFGPAVMHEQGIGITVEEFKKGVETGTLSGHVGFHESIGMISEAIGWKV
                     DKVTTSMEPIVTDVDRKSPHGFAKAGDVAGCAMKGYGYVDGELKIEMDHPQQIEPEQV
                     GVQTGDYVIIKGTPNINLVNSPEVPGGIGTIAMCVNTIPMIINAKPGLHTMLTIPAPR
                     AIMGDMRDLIDPECKIVK"
     gene            12361..12666
                     /gene="ortA"
                     /locus_tag="PKH42_01960"
     CDS             12361..12666
                     /gene="ortA"
                     /locus_tag="PKH42_01960"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOA19223.1"
                     /translation="MVKKGEWVLIHRNVLEPAERVPHVPDDTKKVPLELWTKGYLQED
                     ANIGDEVTVLTRTKRLEKGTLLEVNPCYKHDFGNFVPELLKISEQVKEILFGGADNE"
     gene            12659..14059
                     /gene="ortB"
                     /locus_tag="PKH42_01965"
     CDS             12659..14059
                     /gene="ortB"
                     /locus_tag="PKH42_01965"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HOA19224.1"
                     /translation="MSKDMSYAAVMARRPEIMKNSAGLDFSKFESGSIAFDYERMMKE
                     AGFTIEEIQKIQSEHGVGNTPIIELRNLTALARKIAPEGKGARIFIKDEAANASGSFK
                     ARRASTAVYQAKKLGYKGVVTATSGNYGAAVASQAAMYGLKCIVVQECYDSRGVGQPE
                     IIEKARKCEALGAEVVQLSVGPELFYTFLTILEETGYFNASLYTPFAVAGVETLGYEI
                     AMQFREKFGKDPDVVMATNAGGGNLTGTARGLKKAGAQSQIVAASVDLSGLHMASDTQ
                     FNRKSFTTGHTGFGMPFSTWPDRSDVPRSAARPLRYIDRYLIVHQGEVFYMTEALASL
                     EGLEKGPAGNTSLTAAFALAQELDQDQTIVVQETEYTGAGKHIQPQLSFARANGIEVK
                     FGDPKDEIPGKNIILPSHPSLIKANDADVIAMRKSLIRNALNNYKVTPTEADIEFLAA
                     ETNSSVEFVKETIASL"
     gene            14127..14477
                     /locus_tag="PKH42_01970"
     CDS             14127..14477
                     /locus_tag="PKH42_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HOA19225.1"
                     /translation="MKRADDYQQRRAHLANLTDEELYERFWKLANEIVDPLLELGKKN
                     TTPSVERSILLRMGFSSLEVKPIVEGVMNKGLMGKGAGNVVWRLSKKLGCSVREAGLL
                     LAEGKHWDDVDTLF"
     gene            14489..16684
                     /gene="oraE"
                     /locus_tag="PKH42_01975"
     CDS             14489..16684
                     /gene="oraE"
                     /locus_tag="PKH42_01975"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HOA19226.1"
                     /translation="MKLEPNKKLDIKELLKDLDKYEPRRRGWHWREGRNEKRTVGDFE
                     YHETSECLKNSIPLPGSRGFGYIDPQPDCVITTEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHIDSLLEGTNQGIGGIMVTRKQVRASRKACDLIEDEIGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKIMAWADMLQIDGA
                     HNANATAMKAWKVMPELMVQHAINTAFSVRVGIKPENIALSTVPPTAPPAPCMRLDLP
                     YAVALRDLFKDYKMRAQQNTKYMESDTREAAVTHTLNMVISRLTSADIQSTITPDEGR
                     NVPWHYNNIAGTMTAKQALTGMDGFREMVKLDREGPLGKEVRELKERAILFMEEILEV
                     GGYFKAVEGGFFIDSGKYPERNGDGIARQIDGGIGYGFIYERDEDYFAPVSVHYGYNN
                     IPAEFKKASDAIGGDTFEDRSKIQYIDELDEHDNVNVRLKEVEKYYDTNLVKPEVEWR
                     ADGIVLLTIFLPLDERTAEFAAIKCGEKMGLEDVTVVHKQCIHPSEGTYCEVKGRVNF
                     DIDINELVIPEKPDLLSEAEIREDIQNNPMFVVAATVGEDEHSVGLKETLDIKHGGIE
                     GFGIKYEYLGTSCPVEKLVDAAIETNADAILLSTIITHDDVHIKNMKKVHDLAIEKGI
                     RDKVMIICGGTQVTNEIALEAGLDAGFGRGTHGIDVASFLVKKRREKKA"
     gene            16684..18036
                     /locus_tag="PKH42_01980"
     CDS             16684..18036
                     /locus_tag="PKH42_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HOA19227.1"
                     /translation="MKVNVLVAEIGSTTTVVNAFNGIGTGNPAFVGQGQAPTTVSEGD
                     VTIGLKGAIEDLKGNLQADTLTWDDMLATSSAAGGLKMTVHGLVYDMTVRAAKEAALG
                     AGGIIKMITSGRLRKTDLKKIQDIKPNIILVAGGVDYGERDTALYNMEMILSMGLKIP
                     IIYAGNIENQEEVRLMCEEADNQIYIVENVYPKIDTLVVEPTRKIIQDAFEEHIIHAP
                     GMAKVRDLVKGPIIPTPGAVMEAAKVLREEIGDLIIFDVGGATTDLHSVTEGSSEINS
                     ILISPEPVAKRTVEGDLGVYVNVSHIVEKIGMDNLKKEFPDAEELLENYKPIPKTDRE
                     KQFVERLTKEAVHTSLERHAGKLRYFYTASGKKTVAEGKDLTAIQYIIGTGGSLTRLP
                     DKKDILESVKTLAKEHELYPTEAARVLIDEDYILSSLGVLSKSYLSDALYLMKNSLRM
                     "
     gene            18045..19112
                     /gene="orr"
                     /locus_tag="PKH42_01985"
     CDS             18045..19112
                     /gene="orr"
                     /locus_tag="PKH42_01985"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HOA19228.1"
                     /translation="MYPRLVIDLKKVKNNLDRITEMVKGSGSSLMIVTKGYSADMEIF
                     KILDGSDIDYLADSRIQNLKKYEGAKKERVLLRLPMNSETDEVVKYADISLNSELKTI
                     KNLNESAKRQNKIHKILLMIDLGDLREGIFFKNEDEIYHTVEEILNLKNIELFGLGVN
                     LTCYGAVIPKSDNLSILVEIARKIEKKFDIKLQMISGGNSSSIYLIGRKELPEGINNL
                     RVGEAFLLGGETAYSQKLEGFYDDAFTLEAEIVELKEKQSVPIGETGVDAFGNKPVYE
                     DRGIIKRAIIAVGRQDVDPDALHPIDSKIDILGASSDHLILDISKSDMKYKVGDIVNF
                     KLSYSSLLRATTSGYVDRVYK"
     gene            19275..21500
                     /locus_tag="PKH42_01990"
     CDS             19275..21500
                     /locus_tag="PKH42_01990"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOA19229.1"
                     /translation="MESIKRLGIFILIFAFSLVLLLKEPFIGIADNSDYYRVIQPLGF
                     KPEISNRYFYAYNFYTVNDMSSEDIKGSLSNIISPKVENDNEYFSTQFIFIKVSMIIN
                     YLLKIVLGKSPEIFNIKVLGILYAAIYSYGLCLFLTNINFKYKYINFLFLIIALVILC
                     DMGYLLYFNSFFGEAAIIASLMITLGLLTAIIKTESKIKSLFYVILFYIFALALTGAK
                     VANTPIGILIGIFSLALFIVKADWLSRAVILIGSILIICFSIFYYTNAPRWMSQVNNY
                     QSIFFGITKDSNEPEKDLEKLSIPLKYLPLTNTHGFLDHGEFDIYSDEFQKEVYDNAT
                     FLDILKFYILNPSRAVEKLKLSADSSVIIRPSYLGNCSKEDEPERLSFTERFSLWSNI
                     RKNALGYAFYIIVSYSVLFFIINIYEIINNIKQYDYENTAFAFAALLLFLTTMSQFVL
                     PIIGNGEADLQKHMLLFNLCFDIMILVGICWLINNFYTKTVSAVVLTAFVVFCIAIFI
                     QTANEEIKETGTLKIGQYIYLGSYKNEPLKWVVLNKDENGYLLWCDNTVEYMEFDYSD
                     ETNSDNIYGSNNWIESDVRRWLFEFKSNFNDEEKLLIKDVKLKNILSYNNIEKSIGGN
                     KPFYWNSITSYVSQNYNTDAYYNYSAESVFLLDVYQLQKYVYENKISLKKQERYWLRT
                     PYYSSESMVRIVDKDGFVYHKDANVKAGVIPAVYIDENVSAIEGDGTYTSPIAIEKSR
                     R"
     gene            21502..22455
                     /locus_tag="PKH42_01995"
     CDS             21502..22455
                     /locus_tag="PKH42_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017153413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA19230.1"
                     /translation="MKKVSLLIPAYNEEETIPLLYNELNKVIDKISGYEFEILFVNDG
                     SSDNTLNILRDLRKRDSRVNYISFSRNFGKETAMAAGFDYVTGDATVILDADLQDPPE
                     LIEEMIFYWEKGYDDVYAKRRSREGETWLKKFTSAAFYKLLRKMTKIPIQEDTGDFRL
                     LDRRAIESLKKLREKQRYTKGMFSWIGFNKKEILFDRKPRAAGKTKWNYLKLFNLALE
                     GVTSFTTSPLRISTILGILVSIFSIVYMFIVLIKSLIWKDPVQGYPSMMVTILFLGGV
                     QLVCLGIIGEYVGRIFNETKYRPLYIVDEHNNEKIEDGGKL"
     gene            22455..22832
                     /locus_tag="PKH42_02000"
     CDS             22455..22832
                     /locus_tag="PKH42_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOA19231.1"
                     /translation="MKNGISKELAAYLIAGILTTIVNFIVYYAMLFIGIDYKISNTAA
                     FIISVIFAFIINKKYVFLSDKSYFKEFIKFSLGRLFTYALDIGAMIILIEVFSVSEYM
                     AKLWTNILVMISNYLISKFWTFK"
     gene            complement(22971..24233)
                     /locus_tag="PKH42_02005"
     CDS             complement(22971..24233)
                     /locus_tag="PKH42_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA19232.1"
                     /translation="MLSFISIFNLIIFIIFTVFYSYQIFYVFVALFNKSKNFNASINH
                     KYAVVIAARNESAVIAQLINSIKKQNYPGELLDIYVVADNCTDNTAAVAKKAGAIVFE
                     RFNRQLVGKGYALDYAFKNILKSNKCYEGYFVFDADNLLDENYVSEMNKVFDKGYKII
                     TSYRNSKNYDTNWLSAGYSLWFLREAKYLNNSRMILNTGCAISGTGFMVSDEIIRKNN
                     GWKHHLLTEDIEFTIDSALQGEKIGYCGSAVLYDEQPYLFKQSWNQRLRWAKGFYQVF
                     AKYGIDLIKAIFKKRSFCCYDMFVTIMPALFLTLISVAVNLTLFLIGLINIETYPELM
                     NETIGAILLSVLNSYIVLFILGLITTVTEWKNINSSPLKKIKYIFSFPIFIFTYIPIS
                     IVALFKFKKIEWKPITHSIVKTIEEVRQ"
     gene            24572..25828
                     /locus_tag="PKH42_02010"
     CDS             24572..25828
                     /locus_tag="PKH42_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOA19233.1"
                     /translation="MDDDGISQVAILIIVLLISAYFSASETAFQKFNKSRMKNLASNN
                     KKAALVLKLDDDYDNLMSTIIIFKSIVNVTAASLVIFIFSKMFINNEILISTVLAIFF
                     IIILGELIPKGLANIAPEKAAMFSAPTMSIFLQICSPVNFLLAICKEMIYKGFKAEVQ
                     PSVYEDELRTMLDEVENEGVINKNESDLIRSAIEFNETVAEDIYTPRIDIVGVEENES
                     LESIKEKFILSGYSRLPVFSGDMDNIIGVLHEKDFYQGLNRKEKDIKKLISKTIFITP
                     NKKISELLKELQLSKVHMAVVIDEYGGTEGLITMEDIIEELVGEIWDEHDEVIEWFKK
                     IGDDKYLVSCNADIDDMFELLGVEIDEELDITTVNGFITMLFEGIPEEGGRIVYKDLD
                     ITVTKAEAKRVLEIQVERIKKEESLH"
     gene            25933..26508
                     /locus_tag="PKH42_02015"
     CDS             25933..26508
                     /locus_tag="PKH42_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOA19234.1"
                     /translation="MNKLWFGFVFCGLLFSFINKSPETVTNVLFFEIEKSVELILNLL
                     PIMAFWTGLMRIVEKTNILNKLSTLLKPLVRYLFKDVQNDANAVNAILMTLAANLFGI
                     GNSATAFGIKAMQEMQKSNLNKKTATKAMCMFLIINVSSIQLIPLNVIKLRADSGSVA
                     PSEIMVPTLLVTAFSTMVAIIFAKYYEGKEL"
     gene            26505..27020
                     /locus_tag="PKH42_02020"
     CDS             26505..27020
                     /locus_tag="PKH42_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOA19235.1"
                     /translation="MNISDLFLPILIGLILFYGIIKGVDVYSAFVEGAIDGAKSALKI
                     FPYILAIIFAINLFIQSGAESFIVDKLTPITSFIGFPTELLSLSIIKPLSGGGSFGVF
                     QTILDNYGADSYIGRSASIIMGSSETIFYITAVYFGSVGISDYRYTIKAGLLSHAATI
                     FAALLVCKLMF"
     gene            complement(27009..27593)
                     /locus_tag="PKH42_02025"
     CDS             complement(27009..27593)
                     /locus_tag="PKH42_02025"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOA19236.1"
                     /translation="MYFEIIFFSLLSIITAIVVLLIKKRDFDFDRYNIKIRGYKVLIL
                     AAVIKIAAEFLFNKYTNVTLLRILSHNWVIYFGLLYISLLNIKRSYMMLFFLGALFNF
                     AAIAANDFKMPVYVSELLSDVETKKMFLLSGNDLVHSLLTKETKLKFLCDIITLYPPY
                     PFPKTISVGDVFLLSGIFAFWQDLFYESSKILKH"
     gene            complement(27583..>28239)
                     /locus_tag="PKH42_02030"
     CDS             complement(27583..>28239)
                     /locus_tag="PKH42_02030"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOA19237.1"
                     /translation="LFFIPLLLARYTFKLYLDMRKNYFETLNVLVRAIEASDPYTSGH
                     SMRVSAYAEAIARQVGLPQSKIDLIKSAALLHDIGKIGIDKNILNKTGKLKKEEFEKI
                     KSHPEIGATIIADLSYLSNITDIIRHHHERNDGKGYPDGLCHSNISLETSILTIADSF
                     DAMTTDRPYRSALSLESALQEIKNNEGTQFNPDIVDDAIIALKKAFFKIAEEKVEHVF
                     "
     assembly_gap    28240..28339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28516..29139)
                     /locus_tag="PKH42_02035"
     CDS             complement(28516..29139)
                     /locus_tag="PKH42_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19238.1"
                     /translation="MSFEEMEHFLNNIKSVLSSKVIIDSNNEIREIHVLADNSRHSKQ
                     IARDIRTALLSKFNEDVDYKIISVAQIDKNMSFNSDFRLLYEGYINETTPEKIKIKTK
                     LTWDGKEFIGEAEGFKSDKQSLIVAARSTLDAIRQAIEFDCFILEDIQYTKIAGEKAL
                     IVAINQINNGKENISVGASIIEDNQIDSAIKATLNAINRKICLLIKD"
     gene            complement(29304..>29782)
                     /locus_tag="PKH42_02040"
     CDS             complement(29304..>29782)
                     /locus_tag="PKH42_02040"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOA19239.1"
                     /translation="FLEGSGFEVEMTRYDDSGLYTELSGTIRDKKNEDLKNRVELINN
                     SNADLVISIHLNSFPQKQYYGAHVFYQKSNEATTKLAADIIQESMKNILDKSNQRVPQ
                     IKKGIKIMDDTKVPVILIECGFLSNNSEERKLISEDYQEKTAWAIYTGLLKYLNEL"
BASE COUNT        10405 a         4406 c         5735 g         9136 t
ORIGIN      
        1 tagcaattaa gataattaaa agcataatca atctataatc ctaatttctt acaatctttt
       61 taagttatga atttttattg cttgataaga aataaattgt tataatggtt atcaggtaat
      121 ataataagtt cagtataaat aagattaagg acgtgaaaat gaagacattt ataaatgaat
      181 gggttattcc ttcaattatt gctgttgtaa ttgtactttt tttaaacaga tttatattta
      241 ttctggtgac ggttccaacc ggctccatgg aaacaacaat tatgcccggt gacagacttt
      301 acgttaacaa actttttgac ataaaggatg caaaaagggg agatattctt gtattccaat
      361 cagatgagct ggatgcaagg cttgtaaaaa gacttattgg attgccggga gaaactgttg
      421 aaataaatgc aacaggtgaa gtgttcataa atggcgaaaa gctcgatgag ccgtatatca
      481 ataatgcaaa cggagaagaa agaacattca aaatccctga aggcacatac ttttttttgg
      541 gcgataacag accagtatct gtagatgcaa ggtactggag caatcctttt ataccggagg
      601 ataaaattat cgggaaggtc gctttcagat ttttcccctt taaccggata ggtaaggtcg
      661 aataatagaa ctatttacaa catgcagaat aaaagaatct tgacagtata tataattttt
      721 tgtaatgtaa agaaattaag aaaggtaagg tatacagaat gaaataatcc gcttgaaaaa
      781 caattataat gacaattaaa ttaaagattt tttattgtgc attttattgt attacaggtg
      841 gatagtattt taatagctgc tgttacttta ttattactat gcaagattta ttatgcatat
      901 ccttagatat attgtgtaaa gtaaaaatgc tatcctccaa agaaagaaag ggaggatttt
      961 ttatgcttat tattgagtgc agtaaaataa aaaaatatta tggagacaga ttaatattag
     1021 atatagataa tttaaacata tactctgaag acaggatagg tgtggtaggt ttaaacggag
     1081 caggaaaaac tacattaata aatattttaa gccaaaggat agagcctgac aaaggatggg
     1141 ttaagcttag gggtaaaaca gaatactttt cacagttaga ggagccggaa gtttatgaaa
     1201 tcagcagtga aatggcttca aaatttggtg tggaaacatt atggaatgaa aatatgagcg
     1261 gtggagaaaa aacaagattc aaattagcta aagccttaga aaatgaaagt atattaattt
     1321 ttgcagatga gccgaccagc aatatggata ttgaaggtat tgagcttatg gaaaagaagc
     1381 taacggaata caagggtgcg ttgataataa tttctcacga tagaagcttt ttagataaac
     1441 tatgtaataa aattattgaa attgaaaacg gaaagattaa aacttatgaa ggcaattaca
     1501 gtgattacag cagacaaaga cttcatgata aagaaagagc acaatttgag tatgaagaat
     1561 atataaaaga gaaggaaaga ttaggaagtg taatagccga tactaaacaa aagataaaaa
     1621 gtataaaaag accacctaag agaatgggaa attcagaagc gaggcttcat aaaatgggcg
     1681 gtcagaaagc caaggcaaat ttagagcgca aagtaggcaa tttggaaaaa agaattgaac
     1741 atttagaggt gaaagaaaaa cctaaaaaaa tatctaaaat taaaatagat attatgcctt
     1801 ccgattatat tcacagtaaa ataataattg aagggaagaa agtaaacaaa tccttcggga
     1861 ataaaattat attcaaggat gccgagtttt gtatttataa tggttcaaag gtagcattga
     1921 taggtcctaa cggctgcgga aaaagtacct tggtaaagat gataatagat aaggataatt
     1981 caatcaggct gtccaaaggt gcaaagatag gctattttag ccaagatatg aacatattga
     2041 ataacagttt aaatattatt gacaatgtga tggaaagctg taattattcc gaaaattttg
     2101 caaggcttct acttgcaaga cttttattta aaggtgatga tatttataaa aaaattgatg
     2161 tattaagcgg gggagagcaa ataaaagttt catttgcaaa aatattattg caggatatta
     2221 atttattaat attggatgag ccgacaaatt atttagatat taattcatta gaagttatgg
     2281 aggatactct taaggaatat gacagaacgc tcttgtttgt ttctcatgac cgcagcttaa
     2341 tcagtaaagt ggcagaccat attatgacta ttgaaaatta taaaataaaa acatttaacg
     2401 gtacttttgc agaattcaaa gaaatacaaa gtaagaaaaa actagacaaa gatgttaata
     2461 tgcagataat tttgttagaa aacaggcttt ccgatatcgt aggaagactg tctatgcaat
     2521 taaaaaagga agagctagaa gctttggata aagaatatta tgaggtctta gaaaagttaa
     2581 ataaactaaa gacgttaaga acaactcccg tataggataa tcccggagtt gtcggaatta
     2641 ttacttgtgg taggtttcgc ctgaatttat tttgaaagct ctgtaaattt gctctaataa
     2701 aataacttta aacagctggt gggggaaggt cattttggaa aatgaaagat taaagtcggc
     2761 actcttgatt acttctttcc aaagtccgtt gctcccgcct ataataaaag ttatatccga
     2821 aataccttcg attccgaagg tatttaattt ttcggcaaag gcttcggagc taagctgagc
     2881 accgtttata tccaaaacaa ttacataaga atttttggga attttattaa gtattttttg
     2941 cccttctttt atcttaacct gctctatttc tgcaggtgaa aaactttcat ccgccctttc
     3001 atcgggcaca atctcttcaa taaacttgca atattttgaa agacgtttaa tatattcctt
     3061 aattgcttca gtaaaaaact tttcttttat tttgcctacg gatattattg ttattttcat
     3121 ttcaatcctc tgttaatatt aatcatatag tattttttac aatatattat tataccatta
     3181 tattattgaa tttaccagac taagagtaag ttaaatccta cagaatgaaa attaacgctt
     3241 attcatagca aaactccgga tggcactctt agcgtaaacg tttgcaaatt ttgattgcat
     3301 tttgttttaa gctatgctat aatttttata cttttaacaa tatttcatcc aatcaaataa
     3361 tttatcggcg aggtaaataa gatgattaaa ataaaaaaaa taatatgttt ggtaatatgt
     3421 attagtatgt taggaactca agcttttggt ttcagccaag cttataatga atttgatatt
     3481 aaaaaaagta ttgaaaatac ttacggaata aatattataa taccggaagg tgttgattac
     3541 agcgaattta ttgattgcat gtcaatattg gaaaggagta ttaagaaatt tcccgacaat
     3601 attattaaag aaattaccga ctattattta agcaagggaa ttgctacgaa tgtaatatta
     3661 gataaaacag aaataataaa agaattgttt acattatcaa taatagatga aagctcagtc
     3721 aatatttaca taaagacctt ggaaggcagt ctgtattctg aatctcatta tgcatctgaa
     3781 caagttataa cacatgtttt tagtcatttc ataagtgatt atatgcttga aacatatgat
     3841 cttgataatt taaaagatga atttgacaag ttaaatgagg gatttgaata cggtacctgg
     3901 agcgaggagt attcaaaagt tttcgtcaac cggcattccg caatcagttt tgagaatgat
     3961 attgcacact taatatggta tgcggaagca aatccgttca tacttcgtaa tataaacggc
     4021 ggggattatg caataataca tgaaaaactc agattgattg ccgaagaatt cagtaaaatc
     4081 tttaattcaa ttacagaaaa tacaaaactc tggcttgatg caatacctca aactcctcag
     4141 gattgggcta aggacacaat tacggaaatg aaaaatcagt ctcttattcc ggatgaattc
     4201 aacgggctgt atgaagctta tattaaaaga ggagatttct ataaactggt tttaaacatg
     4261 cttcacatta aattaggaga aaatgaatta aacaaatact ttaatttaac ggattatgaa
     4321 gagcatgtgg cattagaccc tcttagggga gaagtttatg taaccgatgg gttgaattat
     4381 acttcttatt ataattattt ggcaaactca agcaatgcat tttatgaagc atatcagatg
     4441 ggaattttaa acacagattc cttttatgag ccggacgaat atatgacaag acttgaaata
     4501 gcaaaactat tagcatatat agccaatgaa ttgggtatga atatttcaga atttcagacc
     4561 gtatctttta atgatataga gcaggttgca gaaaatgaaa aaccctatat atatattgca
     4621 gctgataaag ggtttataaa aggagacgga ttaaacttca aaccgtttaa ttattgtaca
     4681 tatcaggaag cgtatttaat cctaaataga ttttataaca gcctatacga ttatattcaa
     4741 taatataaaa ttgaggtagt attatgtttg acttaaaaag tattgttata ggccttgtat
     4801 taatgataaa aaaatattcc tgtttaagtc ttaccattta tgaagaagca aatgaggagg
     4861 attataaaaa cggtataatt gcacttccca taataggact tgccattgga ttagtagcat
     4921 ttattattgt ttcctttaga tatatatatg acggtttttt tatcagttct gcagttcttt
     4981 tatattactg tgttgtaaca aaaacaacca atataaagga tgtttacaga actttaaatt
     5041 attatataaa gcctgcaaat caaaccgata atatatcagg aattattgga acggttttaa
     5101 tctgtttcat gtacctttct ctatttcgag ttgtacccgt aacttctttg attattatgc
     5161 ctgctgcagg attttcaaat ttgatgatac ttagcaaggt tataaaaaga gatttagaaa
     5221 atacttcgat actcaaatac tgcggcaatt accatataat agctgccttt ggcatatctt
     5281 ttctatctgc ggcaatacta aactataagc ttataatttc gctatctctt atgtttatgc
     5341 tctccggatt aattgtaagc atattggata aaaggataaa aaatgtgccg tcatcagttg
     5401 aagggtttat aatagaagtg tcacaaataa tgtttttgat tattacttat ataataaggt
     5461 ttggggacac acctctctag tgaagggata gtccctgcag gagcgaggaa tgtccccgtt
     5521 cttagttggg ggacacacct ctttgaccaa ttgttgacaa attattttta tagtagtata
     5581 atcaaaaaaa attgagcttt acgagtttca aaaataggaa ctcgttttta cttatatatt
     5641 ctcggaggag taaaatggaa aataaaaaca gaatattcag cggcatgcag cccacaggct
     5701 cattgacttt gggaaattat ctgggagctt tgaaaaattg ggtggctctg caaaatgaat
     5761 atgactgtta ttattgtgta gttgatatgc atgcgataac agtacctaag gagcctaagg
     5821 atttaagaaa aaatacatat gaagtattag ccaattatat agctgccggg ttaaatacgg
     5881 aacaggtaac attatttatt caatcacacg tttcggcaca tgcggaactt acatggatat
     5941 tgaattgttt ttcttactat ggggagcttg gcaggatgac acaatttaaa gacaaatcca
     6001 aaaagcagga ttccaatgcc ataacggcag gactttttac ctaccctgtt ttaatggctg
     6061 ctgacatact tttatatcaa gctgatttgg ttcctgtggg agaagaccaa agacagcact
     6121 tagaaataac aagggatatt gcacaaagat ttaataacag attcggtgaa acatttgtag
     6181 ttcctgagcc ttatatttcg accgatgtag caaaaataat gagcttgcag gaccctgaga
     6241 gcaaaatgtc taaatcagat tcaaatgaaa attcatatgt gctcattacc gatagtcctg
     6301 atgttataag aaggaagttt aagcgttcgg ttacggattc aataggcgtt gtaaattata
     6361 cggatgagca gccgggagta aaaaatttaa taaatattta ctcgaaaatt tcaaatataa
     6421 agcctgaaga aattgtatcc aattatgaaa gcaagggata tgctaaatta aaagaagatg
     6481 ttgcagaagt tgttattgaa gaactgaagc ctatacagga gagaatagat tatttgttga
     6541 aaaacaaaga ttatcttgaa aagatatata atgccggtgc gcaaaaagca gaggttgtag
     6601 caaataaaac cttaaaaaaa gctcaaaaga aagtcggatt cataccaaga tagacagggg
     6661 tatcgatagg actaatgacg gggactcacc tttaccttca tatttgaggg attaaaggac
     6721 gttccctttt tttaattgag tcagggtgac gacaaaaaaa acttgcaaaa ttttattaaa
     6781 aagattaaac taaactgtaa aaatttaata gaggggcgac ataactctaa tgcagagcaa
     6841 gccctgctta gtcagaagag gggaggattg tccccgtaga ggtaattatg aagaataaaa
     6901 ttattaatat agcggtaatc gcccatgttg atgcgggaaa atctactctg gtggatgctt
     6961 ttttgcagca aagcggcgtt tttagagaaa atcaggaagt ttccgagcaa ataatggatt
     7021 caaatgattt ggagagagaa agaggaataa ccatttattc aaaaaattgt tcggtaatgc
     7081 ataatgatat aaaaattaat atagttgata caccggggca tgctgacttt tcatcagaag
     7141 tcgaaagaat tattaagact gtggatacgg taattttgct ggtggattcc atcgaaggac
     7201 caatgcctca aacgcgattt gtgcttaaga aatccctcga aataggaata aagcctattt
     7261 tgttaataaa taaaatcgac aaaaaaaatc acagggcaga agaagtcgtt gatatggtat
     7321 ttgatttatt cgtagaattg aatgcaaatg atgaacagct tgacttcccg atactgtacg
     7381 gtattgcaaa acaaggaatt gttcagtatg aattagatga ggaaagccac aatattaatc
     7441 ccttgtttga cactattata aatcacgttc agccttatcc cgacagggat gacgaagatt
     7501 tgcagctgca ggtatcaact cttgcctatg atgattatat aggcagactc ggcataggaa
     7561 gggttaccgc cggaacaata aaagaaggcc agattgtttc ggttgcaaag catgacggca
     7621 gcgttgaaaa gcaaaaaata tctaatattt atgtatatga aggtttaaaa cgtactcagg
     7681 ttaaagaggc gttcagcggc gatatagtaa ttgtttcggg aattgccaat atatctatcg
     7741 gagacaccat ttgcaatgaa aacaaaatta taccgatgga aaccataaaa attgaagagc
     7801 ccacgatgtc aatgaatttt ttaatcaata cgtctccttt tgcaggttta tcaggcaaat
     7861 atgttacaac cagacattta aaagcaaggc ttgaaaaaga gttagaagta aatgtggggc
     7921 ttagggttga agctttggaa ggagccttag acggttacaa agtgtcgggg agaggagaac
     7981 ttcatttgtc aattcttctt gaaaatatga gaagggaagg ttatgaagtt gcagtatcaa
     8041 gacctgaagt tattcttaaa aaaatagacg gtatagttat ggagccgatt gaaagagtga
     8101 ttgtaaatgt gcctgaaata tattccggct ctgtaatatc aaagcttaat ttaagaaagg
     8161 gcattatggt ttcaatggtg cctgacggga attacgtaaa aattgaatat ttggttccta
     8221 ccagaggact tttgggttat cgcacggaat tgataaatga ttcaagagga gaaggtacca
     8281 tggtgagagt atttgaccac tatgaaaaat ttaaaggtga aatacctcaa agaattaacg
     8341 gagtattgat atccaacgac cagggcgaag cagttgctta ttcattgagc aacttggaaa
     8401 gcaggggaat tttatttata aatccgggcg acaaagttta tgaaggaatg attataggca
     8461 tgaattcaag gggagaagat attactgtaa atccctgcaa aacaaaaaaa caatctaata
     8521 ttcgttcatc ggcagctgat gatgcaataa aattatcacc tccaaaaata atgactttgg
     8581 aagctgcatt ggaatttatc aatgatgatg agttggtgga aataacaccg gatgccataa
     8641 gacttaggaa aaaatattta accgaaatag aaagaagaaa atatgcaaat aaaataaaga
     8701 catcaaacta atctaaaaca gatatagcag attgtgacat agcgcagtct ccttcaccaa
     8761 aatatttgct ccctatggtc gcatattttg ggaagtcgtt gcgaatgacc taccatccat
     8821 ttctcagaaa aaggactctg agaaatgggg ttagggcatc tttactccgg ataattcaaa
     8881 ttttcatctt ttatatttaa aagaacttcg ttataaaatt tagcagcttc atatttggcc
     8941 atatttaagt tcatatcctg ataattcccg cacgatatct catcggctcc cggaatatct
     9001 ccttcataat tcattatgaa taagaacatt tccttaataa tttcatatac atccaatgat
     9061 gtcaaatttc cgctaaaaat aacataaaat cctgtcctgc atcccatagg accaaaatat
     9121 attgtgcgcg tgtcgtatac cggatgattt cttaaaaatg ttgcacctaa atgctctatt
     9181 gtatgaagct cagcagtatt tataacaggc tccctgttag gctccttcat tcttatgtca
     9241 aatgtagtaa caatattttc tccaacttta tcttttctcg atacataaat tcctcgtttt
     9301 aattttaaat gatttacttg aaaactctct atttttttca tttgaactcc tttaatgttt
     9361 atctcatata atcctcgatt acgcttagga ttacaaatac atatgcctta catcaaatat
     9421 taatttcatt ttataccgta attatacaaa agtaaactat catcattgta aaaattgaat
     9481 tcttatgata caattaatca ttagaatggg gacaggtcta taggcgagtc tatatagata
     9541 agaatagagg aaggtccccg gtaaaattgg aggggtctat gaaagcaaag gaatgtttac
     9601 tgttgctgca aaaaattttg cagttagtag atgaaggtgt tcatgtactt gacaagcatg
     9661 gcaataccat catatataat gaagccatgt ctgctttgga aaagatggac tcagctgatg
     9721 taatcaagaa accttttaca gaggttttca agggatttaa caccgaaaac agcactcttc
     9781 taaaagcctt gcattctaaa accgtaacga aaaatttaaa gcaaacatat ttgaacaagg
     9841 acggaaaaga gatttctaca attaatacca caatacccgt gataattgat gatgaggttg
     9901 tggctgctgt tgaagtagct aaaaatataa ctaaaattga agaaatgtcc aatacaatat
     9961 taaaactcca tgatgaaata gaaaaaccgg aaatggtaaa acataagaaa gtaaagaaat
    10021 ataattttga caatattatc ggacgcagtg aaaatttctt ggatgttatt gaaagagcga
    10081 aaaaatcttc aaaaaattct gcatcagtat ttatttacgg tgaaacagga acaggtaagg
    10141 agttaattgc tcaaagcatt cattatgcca gcgctagaaa ggataagcct tttaaagctc
    10201 aaaattgtgc tgctcttccg gaatctttac ttgaaggatt attatttggc acaacaaagg
    10261 gggggttcac aggtgcggtt gaccgtcccg gattatttga acaggcaaat ggaggaacgc
    10321 ttctattaga tgaaataaat tcaatgccat atgaattgca gcctaagctg ttaagagtac
    10381 ttcaagaaaa ctatataaga cgggttgggg gttcaactga tattcccatt gatgtaagaa
    10441 ttattactac atgtaatgag cctcccgaaa aaatattaaa agaaggcaag ctaagaaagg
    10501 atttatttta taggcttaat gtaattccaa taaatgtacc tccattaaga gaaagagttg
    10561 atgatattat tttgcttgct aaaatgtttg ttgataaaca caatgagagg tttaacaaac
    10621 acgttacagg tttctcggat gaagtgtatg aatatctctt atcatatgat tatccgggca
    10681 atgcaagaga acttgaaaat atgattatgt cagctgtcag catgtgtgaa gatgatgaat
    10741 atattttgac tgttaatcat ttttccgtgc atcgttcaat tgagcctcgg attgacgatt
    10801 atgaaaaagt agaagattcc ggcattgatg aatatttagg aaatttagaa aaaagaataa
    10861 ttgagaaagc acttttagaa aataacaata acattaccag aacatcaaat gcattaaaaa
    10921 taaagcgtca gaccttacag cataaaataa agaaatataa aattagtgtt taaaagcaaa
    10981 tatttttgca cctagctgca aaaatacttg ctttttttat attatttctg aaatgtttgt
    11041 tatataaaga gggaatatac tggaataagt tgcttatttt gagataaaat aacttggagt
    11101 ttgaaatatt aaagttaaat atcaaataac taataaattt atttatttta atgtttttgg
    11161 cataataatt gcttattaaa taataaatag agtagaaaaa tattaggagg attaacaaat
    11221 gcaaaatgta aaagtaatta tttggggact cggagccatg ggaggcggca tggcaagaat
    11281 gcttctggaa aagaaaggtg ttgacatagt tggtgcagtc ggcagaggtg ccaaattagg
    11341 aaaaagcatg tacgaaatta taggagtaga aagaggagac agacctgaag tactcgttgg
    11401 aactcctgaa gatgttatta aagaaaaagc tgcagatgta gttctatgct gtacggattc
    11461 atttactaaa gtagcatttg aaagactaaa attctgcttg gaaagaaaaa taaacgtagt
    11521 atcaagtgcg gaagaaatgg cattcccgca ggctcagaat cctgaacttg caaaacaatt
    11581 ggatgaaata gctaaagcta acggtgtttc aataattgga accggtatta accccggatt
    11641 aattatggac tacttggttg ttgttatgac aggatgctgc caaacaattg accacattac
    11701 agcaagaaga gttaacagct tatcgccctt cggacctgca gttatgcatg agcaaggtat
    11761 aggcatcact gttgaagaat ttaaaaaagg tgttgaaaca ggaacactat caggtcacgt
    11821 cggattccat gaatcaatcg gtatgatttc agaagctata ggctggaaag ttgataaagt
    11881 aactacctca atggagccca ttgttacaga cgtagatagg aaatctcctc atggatttgc
    11941 taaagcaggg gatgttgcag gctgcgcaat gaagggttat ggatatgttg acggcgaatt
    12001 gaaaattgaa atggaccatc cacagcaaat agagcctgaa caggttggtg ttcaaacagg
    12061 tgactacgta ataatcaaag gaactccaaa tattaatttg gttaactctc ctgaagttcc
    12121 gggaggaatc ggtactattg ctatgtgcgt aaatactatt ccgatgatta taaatgctaa
    12181 accgggatta catacaatgc ttactatccc ggcaccaaga gcaatcatgg gagatatgag
    12241 agatttaatc gacccggaat gcaaaatagt aaaataatga tgacttaatc ctatttttaa
    12301 agcttaaggc gaagaaaaaa caatagtgaa attaataaaa taaaggtaag gagcgtaata
    12361 atggttaaaa aaggtgaatg ggttttaata cacagaaatg ttttagagcc ggctgaaaga
    12421 gttccacatg ttcctgatga tactaaaaag gttcctttgg agttatggac aaaaggatac
    12481 ttacaggaag atgcaaatat cggagatgaa gtaacagtat taacaagaac aaaaaggttg
    12541 gaaaaaggca ctttgctgga agtaaatcca tgttacaagc atgatttcgg caactttgtt
    12601 cctgaattat tgaaaatcag cgaacaagta aaagaaattt tatttggagg tgctgataat
    12661 gagtaaagat atgagttatg ctgccgttat ggcaagaaga cctgaaataa tgaaaaattc
    12721 ggccggttta gatttttcta aatttgaaag cggctcaata gcttttgatt atgaaagaat
    12781 gatgaaagaa gccggattta ctattgaaga aatccaaaaa attcagtctg aacacggagt
    12841 tggcaatact ccaattatag agcttagaaa tttaacagca ttagcaagaa aaatagctcc
    12901 ggaaggaaaa ggagcaagga tattcataaa agatgaagca gcaaatgcat cgggaagctt
    12961 taaagcaaga agagcatcaa ccgcagtata tcaagctaaa aagcttggat acaaaggtgt
    13021 tgttacggct acaagcggca attacggcgc tgcagttgca tcacaggcag caatgtatgg
    13081 gcttaaatgc attgtagttc aggaatgcta tgattcaagg ggtgtaggtc agcctgaaat
    13141 aatcgaaaaa gcaaggaagt gtgaggcttt gggggctgaa gttgttcaac tgtcggtagg
    13201 ccccgagtta ttctacacat tcctgacaat attggaagag accggatact ttaatgcatc
    13261 cttgtatact ccgtttgcag tagccggtgt agaaacattg ggttatgaaa ttgcaatgca
    13321 gttcagagaa aagtttggca aggacccgga tgttgtaatg gcaacaaatg caggaggcgg
    13381 aaatcttacg ggaactgcaa gaggacttaa aaaggcagga gctcagtctc aaatagtggc
    13441 tgccagcgta gatttgtcgg gacttcatat ggcatcagac acacagttta acagaaaatc
    13501 atttacgaca ggacatacag gattcggaat gcctttttca acatggccgg accgctcgga
    13561 tgttcctcgc tcggcagcaa gaccattaag atatattgac agatatttaa tagttcatca
    13621 aggggaagtt ttctatatga ctgaagcatt agcatctctt gaaggattgg aaaaaggtcc
    13681 tgccggaaat acttcactta cagctgcatt tgcacttgca caggaattag accaagatca
    13741 aacgatagtt gttcaggaaa cagaatatac aggcgcaggc aagcatattc agcctcagtt
    13801 gtcatttgca agagccaatg gaatagaagt taaatttgga gacccaaagg atgaaattcc
    13861 gggaaagaat ataattttac cttctcatcc ttcattgatt aaagcaaatg acgcagatgt
    13921 gatcgctatg agaaaatcat taataaggaa tgcactgaat aattacaaag taacacctac
    13981 ggaagctgac atagaattcc ttgcggcaga aacaaattcc agcgtggaat tcgtaaaaga
    14041 aacaattgca tctttgtaga atattaaaca aataggcgta tgaaatatta cgcctatttg
    14101 aaatttaaaa attacaggag gattttatga aaagagctga tgattaccag caaagaagag
    14161 ctcacttagc gaatcttaca gatgaagaat tatatgaaag attttggaaa ttagcaaatg
    14221 aaattgtaga ccctctcttg gaattaggaa agaaaaatac aacaccatcc gttgaaagaa
    14281 gtatattgct tcgaatggga ttttcaagtc ttgaagttaa acccatagtt gaaggtgtaa
    14341 tgaataaggg tttaatggga aaaggtgcag gcaatgttgt ttggagatta tcgaagaaat
    14401 taggctgctc agtgcgtgaa gcaggattat tgcttgcaga aggcaaacac tgggatgatg
    14461 ttgatacact attttaggag ggatgaggat gaaacttgaa cctaacaaaa aattagatat
    14521 aaaagaatta ttaaaagatt tagataaata tgaaccgaga cgccgcggat ggcattggag
    14581 ggaaggaaga aacgaaaaaa gaacagtagg tgactttgaa tatcatgaaa catcagagtg
    14641 tttaaaaaat tcaattcccc ttcccggaag cagaggattt ggatatatag acccacagcc
    14701 tgactgtgta attactactg aaattgcttc aggaagattt gaagatgata taagaagaat
    14761 gcgtatggct gcatggcatg gtgcagacca tcttatggtt ataaggacag ccggtcagtc
    14821 acatattgac tccctgcttg aaggtacaaa ccaaggtatc ggcggtatta tggttacaag
    14881 aaagcaagtg agagcttcaa gaaaagcttg tgatttaatt gaagatgaaa taggtcgtcc
    14941 gataaacttc cactcatata tttcgggtgt tgcaggtccc gacatagcag ttatgtttgc
    15001 cgaagaagga gttaacggag cacatcaaga cccgcaatac aatgttttgt acagaaatgt
    15061 taacatggta agaagctttg ttgatgcttg tgaagctaaa aagataatgg catgggcaga
    15121 tatgctgcaa atagacggag ctcacaatgc caatgctaca gcaatgaagg cttggaaagt
    15181 tatgcctgaa ttgatggtac aacatgctat aaatacagcc ttttcagtaa gagtgggcat
    15241 aaaaccagaa aacatagcat tgtctaccgt accgccaacg gcacctcctg caccttgtat
    15301 gagactagac ttaccatatg cagtagctct tcgtgatttg ttcaaagatt ataaaatgag
    15361 agctcagcaa aatacaaaat atatggaatc ggatactcgt gaagcagcgg taactcatac
    15421 attgaacatg gttatatcaa ggcttacaag tgcagatatt caatctacca tcactcctga
    15481 tgaaggaaga aacgtgcctt ggcattataa caacattgca ggaacaatga ctgcaaagca
    15541 agctcttaca ggcatggacg gctttagaga aatggttaag cttgacagag aaggaccctt
    15601 aggcaaagaa gtaagagaac ttaaggaaag agcaatactg ttcatggaag aaatacttga
    15661 agtcggagga tacttcaagg ctgtagaagg aggattcttc attgacagtg gtaaatatcc
    15721 cgagagaaac ggagacggta ttgcacgtca gatagatggc ggaatcggat acggcttcat
    15781 atatgaaaga gatgaggatt attttgctcc cgtatccgta cattacggtt acaacaacat
    15841 acccgctgaa tttaaaaagg caagtgatgc cataggcgga gatacatttg aagaccgctc
    15901 caaaattcag tatatcgatg aattggatga gcatgataat gtaaatgtaa gacttaaaga
    15961 agttgaaaaa tactatgata caaaccttgt taaacctgag gtggaatgga gagcagacgg
    16021 catcgtattg ttgacgatat tccttccatt agatgagaga acggcagaat ttgctgctat
    16081 taaatgcggt gaaaaaatgg gcttggaaga cgtgacggtt gttcataaac aatgcatcca
    16141 tccatccgaa ggaacttatt gtgaagtaaa aggcagagta aactttgata ttgatataaa
    16201 cgagctggtt atccctgaaa aacctgactt attgtcagag gcagaaataa gagaagatat
    16261 tcaaaataac ccgatgtttg ttgttgcagc tactgtaggc gaggatgaac actcggtagg
    16321 gcttaaagaa acattggata taaaacacgg cggtattgaa ggattcggta ttaaatatga
    16381 atatctcgga acttcatgtc ctgttgaaaa attagttgat gcagccatag aaacaaatgc
    16441 tgatgctatt cttctaagca caataattac tcatgacgat gttcatatta aaaatatgaa
    16501 aaaagttcat gatttagcaa tagaaaaagg catcagagat aaagtaatga ttatctgcgg
    16561 cggcacacag gttacaaatg aaatagcttt ggaagcaggg cttgatgccg gttttggaag
    16621 gggaacccac ggtattgacg ttgccagttt cttagtaaag aaaagaaggg aaaagaaggc
    16681 ataatgaagg taaatgtatt ggtagctgaa attggcagca ccactacagt agttaatgca
    16741 ttcaatggaa taggtaccgg taaccctgcc tttgtagggc agggtcaggc acctacaacc
    16801 gtatcagagg gtgatgtaac aatcgggctt aagggcgcta ttgaagactt aaagggaaac
    16861 ctgcaggcgg atacattgac atgggatgat atgctggcta caagtagtgc tgcaggcggt
    16921 ttgaaaatga ccgttcacgg gcttgtatat gatatgacgg ttcgagcagc taaagaagct
    16981 gcgttaggag ccggaggaat cataaagatg attacttcag gcaggctcag aaaaacagat
    17041 ttaaaaaaga tacaggatat aaaacccaac ataatcttag ttgcaggcgg tgtggattac
    17101 ggagaaagag atacagctct ttacaatatg gaaatgattt tgtcaatggg tttaaaaata
    17161 ccgataattt acgcgggaaa cattgaaaac caggaagagg tccgcctgat gtgcgaagaa
    17221 gcggacaatc agatttatat tgtagaaaat gtatatccca aaattgatac attggttgta
    17281 gagcctacaa gaaaaataat tcaggatgct tttgaagagc atattattca tgcgcccggt
    17341 atggccaagg taagagacct agttaaggga cctattattc ctactccggg agcagtaatg
    17401 gaagctgcaa aagtattaag agaagaaata ggagacttga taatatttga tgtgggagga
    17461 gcaacgacag atcttcattc cgtaacggag ggctccagcg aaataaattc aatattaatc
    17521 agtccggagc ctgtggcaaa aagaacggtt gaaggagacc ttggagtata tgtgaatgtt
    17581 tctcacattg ttgaaaaaat aggcatggat aatttgaaaa aagaatttcc cgatgcagaa
    17641 gaactcctgg aaaattataa gccgattcca aaaaccgaca gggaaaaaca atttgttgaa
    17701 aggcttacaa aggaagcagt tcatacgtct ctggagaggc atgcgggtaa attgagatat
    17761 ttctatactg catcaggcaa aaaaaccgtt gctgaaggaa aggatttaac cgcaattcaa
    17821 tacatcatag gaaccggcgg ctcgcttaca agattaccgg acaagaaaga tattctggaa
    17881 tcagtaaaaa ctttagctaa ggagcatgaa ttgtatccga cggaagctgc aagagtttta
    17941 atagatgaag attatatatt gtcttctctt ggagtattgt cgaaaagtta cttgagtgat
    18001 gctttgtacc ttatgaaaaa tagtttaaga atgtaggtga taatatgtat ccaagattag
    18061 taatcgattt aaaaaaagta aaaaacaatc ttgatagaat aaccgaaatg gtgaaaggct
    18121 caggcagctc gctgatgatt gtaacaaaag gttattccgc cgatatggaa atattcaaaa
    18181 tattagatgg ctcggatata gattatttgg cagattcacg catccaaaac ctcaaaaaat
    18241 acgaaggagc taagaaggaa agagttttat taagattgcc catgaattcc gaaactgatg
    18301 aggtagtgaa atatgcagat atttctctaa actcggaatt aaagacaatc aaaaacttga
    18361 acgaatcagc aaaacgacaa aacaaaattc ataaaattct tctgatgatt gacctgggag
    18421 acttaagaga aggtatattc tttaaaaacg aagacgaaat atatcatact gttgaagaaa
    18481 tattaaatct aaaaaatatt gagttgtttg gattaggtgt aaacctaacc tgttatggag
    18541 cagtaattcc aaaaagtgat aatctttcaa tactcgttga aattgcaaga aagattgaaa
    18601 agaaattcga tataaaatta caaatgatat ccgggggaaa ttcaagctcc atatatttga
    18661 taggaaggaa agaactaccc gaaggaatta ataatttaag agttggagag gctttccttt
    18721 taggcggtga aactgcttac agccagaagc ttgaaggatt ttatgacgat gcatttactt
    18781 tggaagcaga aatagttgaa ttaaaagaaa aacaatctgt tcccataggt gaaaccggcg
    18841 tagatgcttt tggcaataaa cctgtatatg aagacagagg tataatcaaa agagcaatta
    18901 tagcggtggg gaggcaggat gttgaccctg atgccttgca cccaatagac tctaaaatag
    18961 atattctggg agcaagctct gaccacttga tactcgatat ttcaaagtcg gacatgaaat
    19021 ataaagtagg agatattgtt aactttaaac tcagctattc aagcttgtta agagcaacaa
    19081 caagcggata tgtagataga gtatataaat agaaaatttt agggcgattt cttttcgcgg
    19141 tgccattttc gtggtgccag gcaaccaagt ttgctgcctg gcaccccaat aaggctcttt
    19201 ttttatgtcg atatatattt acaaaaaaaa ttatctatgc cataatatta ctatatcgtt
    19261 aggttggagt taaaatggaa agtataaaaa gattgggcat tttcatatta atatttgctt
    19321 tctccttagt cttgttatta aaagagcctt tcataggaat tgccgacaac agcgattact
    19381 acagagttat tcagcccttg ggatttaagc ctgaaataag caacagatat ttttatgcat
    19441 ataattttta tactgtcaat gatatgtcaa gtgaagatat aaaaggctct ttgtcaaata
    19501 ttatcagtcc gaaggttgaa aatgacaatg agtatttttc aacccaattt atttttatta
    19561 aagtatcaat gattataaat tatttattaa aaattgtatt aggaaagtcg ccggaaatat
    19621 ttaatataaa agtacttggt attctttatg ctgcaattta ttcttacgga ctatgtttat
    19681 ttcttacaaa tataaatttt aaatataaat acataaattt tttatttttg ataatagctc
    19741 ttgtaatttt gtgcgatatg ggatatttgc tatattttaa ttcttttttc ggagaagcag
    19801 ctattattgc ttctttaatg ataacattgg gcttgcttac tgcaattata aagactgaaa
    19861 gtaaaattaa aagtcttttt tatgtaattt tattttatat atttgcctta gcactaaccg
    19921 gtgctaaggt tgcaaacact ccaataggga tactgatagg tatattttca cttgccctgt
    19981 ttattgtaaa agcagattgg cttagcagag cggtaatttt aataggctct attttgataa
    20041 tttgtttttc gattttttat tatactaatg caccgcgatg gatgagtcag gttaacaatt
    20101 atcaaagtat tttcttcggt attacaaaag attcaaatga gccggaaaag gatttggaaa
    20161 agctttcaat tcctttaaaa taccttcctc ttacaaacac acacggattt ttggaccacg
    20221 gggagtttga tatttacagc gatgaatttc aaaaggaagt atatgataat gcaacatttt
    20281 tggacatact taaattttac attttaaatc cttcaagagc cgttgaaaag cttaagctgt
    20341 cagctgacag ctcggtaata ataagacctt cttatttagg aaactgttca aaggaggatg
    20401 aaccggaacg gctgtcattt accgagagat tttctttgtg gagcaatatt cggaaaaatg
    20461 cattgggcta tgccttttat ataatagttt cttattcagt cttatttttt ataataaata
    20521 tatacgaaat tatcaataat ataaaacagt acgattatga aaacactgca tttgcttttg
    20581 cagcactact attgtttttg acaaccatga gccaattcgt gcttcctatt attggaaatg
    20641 gtgaagctga tttgcaaaag catatgcttt tgttcaacct ttgctttgat ataatgattt
    20701 tagtgggcat atgttggctt ataaacaatt tctacacaaa gacggtttca gcggttgttc
    20761 taacggcttt tgttgtgttt tgcatagcaa tatttattca gactgctaac gaagaaataa
    20821 aagaaacagg gacattaaaa ataggacaat atatttattt aggcagctat aaaaatgaac
    20881 ctctaaagtg ggtagttttg aataaagatg aaaacggcta tcttttatgg tgtgataata
    20941 cggttgaata tatggaattt gattattcag atgaaacaaa ttcagataat atttacggct
    21001 caaataactg gattgaatca gatgtgagaa gatggctctt tgaatttaag agtaatttta
    21061 acgatgaaga aaaacttctt ataaaggatg ttaaactaaa aaatatatta agctataaca
    21121 atattgaaaa aagtatagga ggcaacaaac ctttttactg gaattctatt acttcatatg
    21181 tcagtcaaaa ttataatact gatgcatatt acaattattc cgcagaaagt gtttttctac
    21241 ttgatgtata tcagctacaa aaatatgtat atgaaaataa gataagttta aaaaagcaag
    21301 aaaggtattg gcttagaact ccgtattaca gcagtgaaag catggtgaga atagtcgata
    21361 aggacggatt tgtttaccac aaggatgcaa atgttaaagc aggcgtgatt cccgcagtat
    21421 acatagatga aaatgtcagt gctatagagg gtgacggaac ttatacaagc cctattgcca
    21481 tagaaaaatc aaggaggtaa tatgaaaaaa gtcagcttat tgatacctgc atacaatgaa
    21541 gaggaaacaa tacccttatt atataatgaa ttaaataagg tgattgataa aatttccggc
    21601 tatgaattcg aaatactttt tgttaatgac ggcagcagcg ataacacttt aaatattctc
    21661 agagatttgc ggaaacgaga ttcaagggta aattacatta gcttttcaag aaatttcggc
    21721 aaagaaacgg ctatggctgc aggatttgat tatgtaacag gtgacgcaac ggtgatactg
    21781 gatgcagatt tacaggaccc tcccgagctt attgaggaaa tgatttttta ttgggaaaaa
    21841 ggatatgatg atgtatatgc aaaaagaaga agcagggaag gggaaacgtg gcttaaaaaa
    21901 tttacttctg ccgcgtttta taaactgctc cggaagatga ccaaaatacc tattcaagaa
    21961 gatacaggcg atttcaggct tttggacaga agggcaattg aatctttaaa gaaacttcgg
    22021 gaaaaacaaa ggtataccaa gggaatgttc agctggatag gctttaacaa aaaagaaata
    22081 ttatttgaca ggaaaccaag agcagcaggc aagactaaat ggaattatct gaagcttttt
    22141 aatttggcct tggagggggt tacttcattt acaacatcac ctttaagaat atccaccatc
    22201 ttaggtattc tggtatcaat tttcagcatt gtttacatgt ttattgttct tattaagagt
    22261 ttaatttgga aagatccggt acaaggatat ccttcaatga tggttacaat tttattcttg
    22321 ggaggagttc agttagtgtg tttgggaatt atcggcgaat atgtaggcag aatttttaat
    22381 gaaacaaagt atcgacccct ttatattgta gatgagcaca acaacgaaaa aattgaagac
    22441 ggcggaaaat tgtaatgaag aatgggattt caaaggaatt ggctgcttac ctcatagcgg
    22501 gaatactaac cacaatcgta aacttcattg tttattatgc aatgcttttt atcggtatcg
    22561 actacaaaat atctaataca gctgcattta taatttcagt aatctttgca tttataataa
    22621 ataaaaaata tgtttttcta agcgacaaat catattttaa agaatttata aaattttcat
    22681 tgggaagatt gtttacatat gccttagaca taggagctat gattattttg atagaagttt
    22741 tttccgtaag cgaatatatg gcaaaattgt ggacaaatat actggtaatg ataagcaatt
    22801 atttaataag caaattttgg acatttaaat aaggaattct ccagaattct cgcagtctcg
    22861 ttcaccaaaa tatcagctcc ctccggtcgc atattttggg aactcgttgc gaatgaccta
    22921 ccgctatttt ttctgcgctt tggcttgaaa aaatagggtt agggcatctt ttattgtcga
    22981 acttcttcga ttgttttaac gatgctgtgg gttataggtt tccattcaat tttcttaaat
    23041 ttaaataatg caactattga aataggtata taggtaaaaa taaatatcgg aaatgaaaat
    23101 atgtatttta ttttctttag gggggatgaa tttatatttt tccattcggt aacggttgtg
    23161 ataagcccta atataaacag tactatatag gaatttaaca ctgataacaa tattgcacct
    23221 attgtttcat tcattagttc agggtatgtt tctatattaa tcaaacctat caaaaagaga
    23281 gtcaaattta cagctacgct tattaatgtc aggaatagtg caggcattat cgtaacaaac
    23341 atgtcataac agcaaaagct tcttttttta aaaattgcct ttatcaaatc aatgccatat
    23401 ttggcaaata cctgataaaa ccctttagcc catcttagtc tttggttcca tgattgttta
    23461 aacaggtaag gttgttcatc ataaagaaca gcactgccgc aatatccgat tttttctccc
    23521 tgcaatgcac tgtctattgt aaattcaata tcctctgtga gcaagtgatg cttccacccg
    23581 ttgtttttgc ggattatttc atcgcttacc ataaaacccg tgcctgatat tgcacaacct
    23641 gtatttaata tcatccttga attattcaga tatttggctt ctcttaaaaa ccaaagagaa
    23701 tatcctgccg atagccagtt tgtatcataa tttttggaat ttctgtagct ggtgattatt
    23761 ttataaccct tgtcaaatac tttgttcatt tcactgacat aattttcatc caacaaatta
    23821 tccgcatcga atacaaagta tccttcatag catttattgc tttttaatat atttttaaat
    23881 gcgtaatcaa gagcgtaacc cttccccaca agctgcctat tgaatctttc gaatactatt
    23941 gcacctgctt tctttgccac cgctgcagta ttgtccgtgc aattgtctgc cacaacgtat
    24001 atatccaaca gttcaccggg atagttttgc ttttttatac tatttattaa ctgagcaata
    24061 acagcacttt cgtttcttgc agctatgacc accgcatatt tatgatttat tgaagcattg
    24121 aagtttttgc ttttattaaa caatgccaca aatacgtaaa atatttgata cgaataaaaa
    24181 actgtaaata taataaatat tattaaattg aaaatactta taaacgataa caatttatac
    24241 ctccatttat tttctaccgt gactataaac atttccatta taaaaatcaa ttaagcattc
    24301 cttaaatatg ttttaaatat accttaatcc taattgtgca atatcatgat aaaatgcact
    24361 tatttggttt ataatgttaa taattcttgt tatatatttg tgaaagtagt tttattgttt
    24421 caataatatg cttttttatg cttttatcgt cttccttaat tttcctaatg ttagtcttgc
    24481 aaatatcaaa gaaggttgaa atgaattgac actttcgtat taatcaatta tactataggt
    24541 aataaaaata aattgggagt gtaaaacaaa aatggatgat gacggtataa gtcaggtcgc
    24601 gatattaata attgttttat tgatatctgc atatttttca gcttcagaga ctgcttttca
    24661 aaaatttaac aaatcgagaa tgaaaaactt ggcatctaat aataagaaag cagcattggt
    24721 tttgaagctg gatgatgatt atgacaacct tatgtcaact ataataattt ttaaaagtat
    24781 cgttaatgtt acagctgcat cattggttat ctttattttt tcaaaaatgt ttattaacaa
    24841 cgaaatttta atttcaaccg tacttgcaat ttttttcata attattttag gcgaattaat
    24901 tccaaagggc ttggctaata tagctcctga gaaagcagca atgttttctg ctcctacaat
    24961 gagcattttt ttgcaaattt gttctccggt caattttctt cttgcaatat gtaaggaaat
    25021 gatttataaa ggttttaaag ctgaagttca accttctgtt tatgaagatg aattgcgaac
    25081 catgctggat gaggtagaaa atgaaggagt aataaataaa aatgaaagtg atttaattcg
    25141 ctcggcaatt gagtttaatg aaacggtggc agaggatatt tataccccaa gaattgatat
    25201 tgtaggtgtt gaagaaaatg aaagcttgga gtcaataaag gaaaagttta tattgagcgg
    25261 ttattccagg ctccctgtat tctcgggaga catggacaat attataggtg tacttcatga
    25321 aaaagatttt taccagggtt taaacaggaa agaaaaagat attaaaaaac ttatttctaa
    25381 aaccattttt attacaccaa acaagaaaat atcagagctt ttaaaggaac tgcagctttc
    25441 taaagtccat atggctgttg taatcgatga atacggcgga actgagggat tgattaccat
    25501 ggaggatata atagaagagc ttgttggtga aatttgggat gaacatgacg aagtaattga
    25561 atggttcaag aaaatcggag atgacaaata cttagtatcc tgcaatgctg atattgatga
    25621 tatgtttgag ctgcttggcg ttgagattga tgaggagctt gatattacta cggttaacgg
    25681 ttttataaca atgttgtttg agggtatacc ggaagaaggc gggaggattg tttataaaga
    25741 tttagatatt acggttacaa aggcagaagc aaaaagggta cttgaaattc aggtagaaag
    25801 gattaaaaag gaagaatctc tacattaaaa taaatatgaa ttaataataa agacagacat
    25861 attaattaat atgtctgttt gatttttttg ttgcgcagta tcttttacca attacaaagc
    25921 aggaaggata ttatgaataa attatggttt ggatttgttt tttgtggtct tttattttct
    25981 tttataaata aaagtccgga aacagttaca aatgttctct tttttgaaat agaaaagtca
    26041 gtcgaactaa tattaaattt attacccatc atggcatttt ggaccggttt aatgcgtata
    26101 gtggaaaaaa caaatatatt aaacaaatta tcaacgcttt taaagccctt ggtcagatat
    26161 ttgttcaaag atgtacaaaa tgacgctaat gccgtaaatg caattttaat gacattggct
    26221 gccaatctgt ttggaatagg gaactctgcc accgcttttg gtattaaagc gatgcaggaa
    26281 atgcagaaga gcaatttaaa taaaaaaaca gctacaaagg ccatgtgcat gtttttgata
    26341 atcaatgttt cttcgattca gcttatacca ttgaatgtta ttaaattaag agctgattca
    26401 ggctccgtgg ctccttcgga aattatggtg cctactttgc ttgtaactgc attttcaacc
    26461 atggttgcaa taatatttgc caagtattat gaagggaaag aattatgaat atatcagatt
    26521 tatttttgcc tatactgata ggtttgatat tgttttacgg aataataaag ggtgtagatg
    26581 tatattctgc atttgttgag ggagcaatag acggcgcaaa gtcggctctg aaaatatttc
    26641 cttatatatt agcaatcata tttgcaataa atctatttat acaatcagga gctgaatctt
    26701 ttattgtaga taaacttaca cctataactt catttatagg atttccgact gaactattat
    26761 cgctgtcaat tattaaaccc ttgtccggag gaggttcatt cggtgtattt caaacaatac
    26821 tagataatta cggagctgat tcatatatag gaagaagcgc atcaataatt atgggctcat
    26881 cggaaacaat attctatata accgcagtat atttcggctc ggtcggtatt tccgattaca
    26941 gatatacaat aaaggcgggg cttctatcac atgcagcaac aatctttgct gccctgcttg
    27001 tttgtaaatt aatgttttaa aatctttgag ctctcataaa acaagtcttg ccaaaatgca
    27061 aaaatcccgg ataataaaaa cacatcgccg acacttatag tctttggaaa aggataggga
    27121 ggatataatg taattatatc acaaagaaat tttaattttg tttctttcgt caatagtgaa
    27181 tgcactaaat catttccgga aagaagaaac attttttttg tttccacatc tgaaagcagc
    27241 tcagatacat aaacaggcat tttaaaatca tttgctgcaa ttgccgcaaa attaaataag
    27301 gcacctaaaa aaaacaacat catgtaagac cttttaatgt ttagtaatga aatataaaga
    27361 agtccaaaat aaatgaccca gttatgagat aaaattctaa gcaaggttac gtttgtgtat
    27421 ttgttaaaca aaaattcagc agcaattttg attactgccg ccaatattaa taccttatag
    27481 cctctgattt ttatattgta tctgtcaaaa tcaaagtctc ttttttttat cagtaaaact
    27541 acaattgctg ttattattga tagtaaggaa aaaaatataa tttcaaaata catgttcaac
    27601 cttctcttca gctattttaa agaatgcttt cttcaaagcg attatcgcat catctacaat
    27661 atcagggtta aattgtgtac cctcattatt tttgatttct tgaagagcag attcaagact
    27721 taaagcactt ctgtaaggtc tgtctgttgt catagcatca aaggaatcgg caatggtcag
    27781 tatggacgtt tctaatgata tattggaatg gcataatccg tcagggtagc cctttccgtc
    27841 atttctttca tggtgatgtc gtattatatc tgtaatattt gataaatagc ttaaatctgc
    27901 aattattgtt gctccgattt ccggatgaga tttaatcttt tcaaattctt ctttttttaa
    27961 ttttcccgtt ttgttcagaa tatttttgtc tataccgatt ttacctatat catgaagcaa
    28021 ggctgcgctt tttattaaat caatcttgct ctgaggcagt cccacctgtc ttgctattgc
    28081 ttccgcatat gcacttacac gcatggagtg tccgcttgtg tatgggtcgc ttgcttctat
    28141 ggcacgaaca agaacattta acgtttcaaa ataattctta cgcatgtcca aataaagctt
    28201 aaatgtgtat cttgcgagta gaaggggtat gaaaaacaan nnnnnnnnnn nnnnnnnnnn
    28261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28321 nnnnnnnnnn nnnnnnnnnc ccagctaaat ccagcgccgg cagctaatac cattgctact
    28381 attgttgcta atataggaaa taactttttc atctgaaacc tccacagata ttctctgctc
    28441 agttcgctag tagaacagga aggtttcgct acgctaattc tttaaataat tatttgatgt
    28501 taagggtcag ccctatcaat cttttatcaa taaacagatt tttctgttaa ttgcatttaa
    28561 tgttgctttt attgcagaat caatttggtt atcttcaata attgatgcac cgactgaaat
    28621 attttctttg ccgttattaa tctgattaat tgcaacaatc agagcttttt ctccggctat
    28681 tttggtatat tgaatgtctt ctaaaatgaa acaatcaaat tcaatagctt gtctgattgc
    28741 atccaaggtt gatcttgcag ctactatcag ggattgctta tctgatttaa agccctcggc
    28801 ttctcctata aattctttgc catcccaggt gagtttagtt tttattttaa ttttttccgg
    28861 agttgtttca tttatataac cttcgtatag aagtctaaaa tccgagttga aggacatatt
    28921 tttgtctatt tgtgctacgc ttattatttt ataatcaaca tcttcattga acttcgaaag
    28981 cagtgcagtg cgtatatctc ttgcaatttg cttggaatgc ctgctattgt cagccagtac
    29041 atgtatttcg cggatttcat tgtttgaatc gattataact ttagatgaaa gaacagattt
    29101 aatattattt aaaaaatgct ccatttcttc gaaactcata tttatcctcc aagtattgtt
    29161 aattaattat aatataatac tattagaatt acaacattta tttagaaatt ctctaaaatt
    29221 ttcacatttt tttcaataat attacaaaaa aaatacacta ttccataccg caaacgaaaa
    29281 gtgtatttaa tttgattgat taattataat tcatttaaat atttaagtaa accagtatat
    29341 atagcccaag cagttttttc ctgataatct tccgatatta gttttctttc ttcgctgttg
    29401 tttgataaaa atccgcattc gattaaaatc accggaacct tagtatcatc cattattttt
    29461 atgccctttt taatttgagg cacccgttga ttactcttgt caagaatatt tttcatgctt
    29521 tcctgtatta tatcagctgc caattttgta gttgcttcat tgcttttttg ataaaaaaca
    29581 tgagctccgt agtattgttt ctgcggaaag gaatttaaat gtattgatat tactaaatct
    29641 gcatttgagt tattaatcag ttcaactctg ttttttaaat cttcattttt tttatctctg
    29701 attgtgccgg aaagttctgt gtatagaccg ctgtcatcat atcttgtcat ttcaacttca
    29761 aatccgcttc cttccaaaaa ac
//
LOCUS       DAOPUE010000011        28883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1575_length_28883_cov_10.034029, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000011 DAOPUE010000000
VERSION     DAOPUE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 28883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28883
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_28883_cov_10.034029"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1490
                     /locus_tag="PKH42_02045"
                     /pseudo
     CDS             117..1490
                     /locus_tag="PKH42_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
     gene            complement(1642..2124)
                     /locus_tag="PKH42_02050"
     CDS             complement(1642..2124)
                     /locus_tag="PKH42_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOA19240.1"
                     /translation="MNTLIAYGTKYGCAEKCAIELSNELDGMVDTVNLKETNKIELAK
                     YDRVIIGGSAYIGKIQKEVTDFINLNLNELMKKEIGLFICGMQEGEELKKEIAENFPE
                     QLLEKAISVKHFGGEFIFRKMNFFERAIVKKISKVTSDKSDIKHDNIKKLAIELCNMN
                     "
     gene            complement(2138..2524)
                     /locus_tag="PKH42_02055"
     CDS             complement(2138..2524)
                     /locus_tag="PKH42_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA19241.1"
                     /translation="MKLVLKVLGIVFLLFVVVSVLSMFYLSRGLEEGLNVNIAPVDLI
                     LKEDGVYKGSYDFGRWSNELTITVKNNRITKIEITDDMMFVNPDVRNELFKRVMEKQN
                     TTVDAVSGGTVSCKAYLKSIENALNN"
     gene            complement(2541..3137)
                     /locus_tag="PKH42_02060"
     CDS             complement(2541..3137)
                     /locus_tag="PKH42_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOA19242.1"
                     /translation="MVTRREKEIEAMKELIISTAKEIIAREGFDNLSIRKIANKIEYS
                     PSIIYHYFKDKEEIVNIVMRRGYMKIVNAVYAADIESLTPRERLKRMTINYIEEALKM
                     PDEFLAAQINQSPQAHKHTSYLYKGASKEKPALGALYNCIKEIYRDADDEQIELKAQI
                     IAASTMGLIIKLVLEKNIGEEQRQNIINYFIEEVILKI"
     gene            complement(3287..4042)
                     /locus_tag="PKH42_02065"
     CDS             complement(3287..4042)
                     /locus_tag="PKH42_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOA19243.1"
                     /translation="MADFIKSNYIIEEKVKLKEIPAIIFRPRDAKEPIPTVVFYHGWS
                     SNKETQRMRGFILASVGYQVVIPDAIYHGERIPLADYSREAAIEYFWDVVFKNVEEYS
                     IIKNELVSKHAADSNRIAIMGNSMGGFTAGGIFTHNKDIRALIVLNGCCWWENFNRNA
                     DVTEKFERVEERVKELDPMNHLELLTDRPILLLHGDSDTSVPIESQNIFYNKLSYMYK
                     NKERIRLIEYHNLNHFVTTNMMEESINWLGRYL"
     gene            complement(4107..4826)
                     /locus_tag="PKH42_02070"
     CDS             complement(4107..4826)
                     /locus_tag="PKH42_02070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOA19244.1"
                     /translation="MKLNLFVTAITPGIALALTFYWFDRHDREPISMLLKVFVLGALY
                     VIPAILIENFLLFFNIFGGILGAAYTAFIVAGLTEEYIKRKVVLKYVYFNPVFNEKLD
                     GIVYCVMSALGFATFENIMYVVFLFSDVESVGYYRAIISVPAHMLFAVTMGYYLSLAK
                     FSSTPEEHRYFFKKSLTVPAIMHGIFDFILMSNIQPLMFLFIPFVIYLWTANLKKLNI
                     YYNESKFYANNNNEFNYFDDE"
     gene            complement(5129..5680)
                     /locus_tag="PKH42_02075"
     CDS             complement(5129..5680)
                     /locus_tag="PKH42_02075"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOA19245.1"
                     /translation="MRSIAIIADDLTGASDTGIQFRKHGLKTKVVVDANKLDLFLESD
                     EVLAINSNTRPLKYYNAYRKVYEISCILKKAFYASCKMVSHANIRLVDFDCSVNIFGT
                     KIYPGDIIFCDQYGALIIPEGTLYYLVEACRKIAAAELPVIQNCKAVILNGKKAKAQD
                     LVLWRKQMEQLRLELKEEIKSLN"
     gene            complement(6175..8082)
                     /locus_tag="PKH42_02080"
     CDS             complement(6175..8082)
                     /locus_tag="PKH42_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA19246.1"
                     /translation="MSSELAIIAPYPELAEMAKQVCDELEIKADIAVGDLEEGVKLAK
                     KFVKSGAEIIISRGGTATAIMEKVGEPVIEIMVSPYDIIRAVASAKKIGNSIGIIGFK
                     NVIYGCESLGEILDVRIKELEIKDESEVLDVIEKAKKDNIQVIVGDAVSVRYSKKQGL
                     KSILVYSGKESVSMALREAQELASVRRKEKTRAEQFKAILDFTYEGIIATDSEGIVSL
                     VNPAAEKILGKNKTAMIGQTADHVLPSIALGRVQEKGKPLLGEPHRVGNRMIVHNLIP
                     LNINNVIEGTVITFQEASHFQAIETRVRRELYLKGHVAQYTFEDILTDSPIMKKVIKQ
                     AKQFSKADTTVVITGETGTGKEMFAQSIHNDSNRRNGLFVAINCASVPENLLESELFG
                     YEEGAFTGARRGGKKGLFELAHEGTLFLDEIGELSIKLQARLLRVLQEKAIIRVGGDT
                     VIPVNVRIIAATHRNLEESVSKGLFRQDLYYRLNVLRMSLPPLRERLDDIPLLIDELL
                     KKICKRTYKPRPIFSDKVLSKFCSYDWPGNVRELENILERIVALKDGAVVQLEDIDEM
                     RKLENQKILSEKVYKDKKVELKGTLEDMEKEIISITLNLTNQDKEKACNLLGISQTTL
                     WRRLKKWNMSD"
     gene            complement(8164..>9264)
                     /locus_tag="PKH42_02085"
     CDS             complement(8164..>9264)
                     /locus_tag="PKH42_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19247.1"
                     /translation="IEIKSGNIDFNEIRIQYENFKKNYEQLLKTLEVKNLEEAKLNKE
                     KLDSLNRTLESCIMQKNQYLGELTYEELKDRIRELGDLSLVRNTAIVESEMTSINAEK
                     IELISEKKLLESNIDKWAEEYKDLDGLFNKIIEIKLKQNGIKTNLDKLAELPPEYKST
                     DEFRRILTEIRNKYGYLINTLASLKEEYYRLEKELPESTYEELSESLKQAERLFEKRL
                     EKGKRLLKIKENFENIKLKMDEQSFIPVITAFSNYLNLLTNGSYKATDIDDDFNLKLK
                     NENEVEMPLSLLSSGTYDCVALALRLAIAEYILGDNKGFLILDDCLVDLDPNRKDTAV
                     KLISRFANKHQVIFTTCSPDTATLLGGYLIKI"
     assembly_gap    9265..9364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9365..10310)
                     /locus_tag="PKH42_02090"
     CDS             complement(<9365..10310)
                     /locus_tag="PKH42_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19248.1"
                     /translation="MIIKKYTTGRFAGIKDVKIEFKDGLNVILGSNESGKSTLVEGIH
                     SVLFKPSRIGRKTAEDKEFHSKFMPYPIGDSIDGEIDLEHNGEIYTLRKEWGADASSK
                     LTMPNKIVIKSDISINDSLKDVYIYGEGTYSSVFFLKQYYLKEAINRIVENKEATGEI
                     SSLLRSTIMELDGVSLESLGQKIQDEIDTLLKRWDIERNYPENNKGINNPYKVGFGKV
                     VESYYAKERIKLEMAKAEEAEKKFNEICVQIKKTEEKIEELKAKKESMEELENDVIQR
                     SILEPQIEQLNKDISALSKINRDWPRSEEKLKQLITELT"
     gene            complement(10300..11415)
                     /locus_tag="PKH42_02095"
     CDS             complement(10300..11415)
                     /locus_tag="PKH42_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA19249.1"
                     /translation="MIKIFHTADVHIGMKFNNYPEPVKGYLKEARLDVLDNMIKFANE
                     EKCNLFVIAGDLFDNITGIDKKTKDRVITALNNFQGECVLVIPGNHDYDNGMIDLWDS
                     FNKAASDKIVIINEECAYSLEDYGLNAVIFPAPCHSKHSSTNNLQWIKEKEIDDNYVN
                     IGIAHGALEGLSPDLDKNYYNMSIKELNNIPMDLWLLGHTHITYPFKSPITNEKIFNP
                     GTPEPDGLDCKHNGQAWIIIVDDNKKTNAKLIQTGTYKFKDKVYTIEDRDDLDKILEE
                     LIKDEPEKTIARITLEGSVDEDVFQYRQEIYGSIEKNIIYLIVEDSDFKMKITTEKIH
                     REFSDGSFPQELLLSLSDDEDTLQLAYELIMEVRNDN"
     gene            complement(11669..11887)
                     /locus_tag="PKH42_02100"
     CDS             complement(11669..11887)
                     /locus_tag="PKH42_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19250.1"
                     /translation="MAVVRTDMYKYDYNYNKDMNYNLKVFCQSEKVNEEYMRVLLFEE
                     KSKFLINKIAALKAMALVPIKWFLTLIK"
     gene            complement(11984..12424)
                     /locus_tag="PKH42_02105"
     CDS             complement(11984..12424)
                     /locus_tag="PKH42_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOA19251.1"
                     /translation="MELEQKIKMLQVIYAGALADSVLRMGKEGILEKVALEKREEQMH
                     TGKYRASQFGIDKPEDVFLVLSELFGCANWSVNSDNEGLKAEATKCMLCALAKKIGAQ
                     SPCNIFCLDPMEGMIRGLNPDINFEVTETLWSGQKCKVNVLNKK"
     gene            complement(12515..13204)
                     /locus_tag="PKH42_02110"
     CDS             complement(12515..13204)
                     /locus_tag="PKH42_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA19252.1"
                     /translation="MESVINNNLTIEYIIENYGKMVYSICRRMIQNKDTADDAAQEVW
                     LEVNKSLKTFRGESKISTWIYTITSRIVLKMAKEEHKYSTYFLSNYFHGEDIEIPDYE
                     DFDKQLWVREMCDKCLTGTLHCLDNESRLAYILRDINMLPYSDIAAIFEKDEASIRKM
                     VSRSRNKLRNFLNDECILKNPDSKCKCRMSKWVKEIQLDEEYKKLRQTVNRINFYRAS
                     EIILTKDYLLK"
     gene            13561..14529
                     /gene="msrB"
                     /locus_tag="PKH42_02115"
     CDS             13561..14529
                     /gene="msrB"
                     /locus_tag="PKH42_02115"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOA19253.1"
                     /translation="MDKELKGNKLIYLAGGCFWGTEKYLGLINGVVKTEVGYANGNTE
                     NPSYEDVCRRNTGHAETVKVVYNPKVIDLETLLKLFYKAIDPVAINRQGYDIGTQYRT
                     GVYYVDKEDEAVIKESLNELQKEYKEPLAVEVTELKNYYKAEEYHQKYLEKNPGGYCH
                     IGELEFYELEKAQKNSKYKVKPKKELESTLTEMQYNVTQNNATEPPFDNEYYDNFEDG
                     IYVDITSGEPLFTSKDKFDSGCGWPSFSKPIDEKVVKEKSDLSYGMIRTEVRTSNSDS
                     HLGHVFNDGPKESGGLRYCINSAALEFIPVDKMEERGYGDLLYLFE"
     gene            14741..15259
                     /locus_tag="PKH42_02120"
     CDS             14741..15259
                     /locus_tag="PKH42_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA19254.1"
                     /translation="MTKTKEVILCGLLLATGIILPMFFHMFGLTGPIALPMHIPVLIG
                     GFLLSPKLALLLGIITPVLSGMLTGMPVMFPMAVIMAFELGIYGLAASLAVRKFNLSV
                     IPSLIVSMIAGRIAAGLTVAILVELFGVKMNPLIYIKGAIITGIPGIIIQLIFIPALV
                     YAIKSYVKIKSV"
     gene            complement(15256..15624)
                     /locus_tag="PKH42_02125"
     CDS             complement(15256..15624)
                     /locus_tag="PKH42_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOA19255.1"
                     /translation="MYNIKIKRVYQDSSEDDGYRILVDRLWPRGISKEKINIHKWAKE
                     ITPSTELRKTFKHDPDTMDEFRVRYNFELDNNEHALDFINLLKDKLKEGNVTLLYAAK
                     NEEFNHAIVLKEWIERMISN"
     gene            complement(15674..16612)
                     /locus_tag="PKH42_02130"
     CDS             complement(15674..16612)
                     /locus_tag="PKH42_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOA19256.1"
                     /translation="MNKIIQFKRKIFTRDNIGYLMIFAAGCLWGTIGLFVNLLNSAGA
                     DTTLTAFMRMFMGCLILIPIMLHKGGVSLFKIDKKGLSQCFMLGILSQALFNYSYITS
                     IRSVGIATAAILLYTAPVFVAIMSRMLFKESIGRIKILALIINIVGCFLMVTGGNLSN
                     IKLSAAGIFFGVAAGFLYSLVTIIGKSTSGDIHPFTVVFYGFLFGGIAIGIFTSPWES
                     IKTVSGLKFWIYSFGYGLIPTVGSYLLYLGGLRKQIEISKTPVIASVEIVVATLIGII
                     VFEEGLNFINMLGIIIIFSSIVIINMKTPYEGARRQ"
     gene            complement(16609..17268)
                     /locus_tag="PKH42_02135"
     CDS             complement(16609..17268)
                     /locus_tag="PKH42_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19257.1"
                     /translation="MARRKRVYSRRRRINYGRVAFLIAVLVVIILLVMKIFSKNRYIS
                     KVEEALEKDITRIAGSISTVSKIDATIYENEGIKYTNQHENIKRIMTFDGSLSEDMDK
                     AGDVKILLENLLISEKTEVTAELPPKDDGYYWIEADIFTKDKVLIFSVEEEYNFDLYY
                     DIENKTVYVKEKYYDEFSKKYNKVKFQGYSVTDEFRNIINSMANTGLDTSGTQIPETD
                     Q"
     gene            complement(17270..17887)
                     /gene="coaE"
                     /locus_tag="PKH42_02140"
     CDS             complement(17270..17887)
                     /gene="coaE"
                     /locus_tag="PKH42_02140"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOA19258.1"
                     /translation="MKQNRPKVIVITGSIGTGKSTAVDIIKEFGFTVLDSDKIVHEGY
                     NIGSELYYKIINHFGEDILKGDEIDRQKLGEIVFNNDKKLNELNNMVHGYVYDKLKEG
                     IEASKEKVIFLDIPLLFETKDIHEPIYDEIWLIYVSEETQRKRLMERAIAENKKPEDV
                     LKIINKQISIKEKVFMADVVINNEGTVEELKENIKALLEIRGMGW"
     gene            complement(17874..20573)
                     /gene="polA"
                     /locus_tag="PKH42_02145"
     CDS             complement(17874..20573)
                     /gene="polA"
                     /locus_tag="PKH42_02145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOA19259.1"
                     /translation="MKKIMILDGNSLLFRAFYALPPLKTKKGQYTNAVYGFLSMLYKI
                     IDTYSPEYLCVAFDPEKPTFRHEKYKDYKANRQKAPNELVQQFKLIRDVLDVHNIKRV
                     EVEGFEADDVSGTFACAAKEQGADVYLVTSDKDYLQLIDENVRVILTKKGITNIVEMD
                     INSMGEEYGLKPEQFVDLKALMGDSSDNIPGVSGVGEKTALKLIQEYKSLDNLYENIN
                     KITGKLKEKLETDKMQAYMSQTLARIITDIPIEFNIEEFKIKKPDIKKLSELYDELEF
                     RQFKKRLVETIPVDDNGQISLFDTDRAETVKNIDIIYIDSEEKLTEIIKNISRKEKAI
                     LKFLLDGERALFSNALALGISDGENIYYIDFEKAEEKKVLNELKEIFESEKIAIWGHN
                     LKNEIIYLMKNNIQINNIQFDSEIGKYLLNPSESSYSLDKIAYEFLNVEIPSENQILG
                     TGVKRKTFKEIDIEQRKAYIFNYLNTIGKVQKQITEDIDELKMTCLYEEIELPLIEVL
                     AYMEFVGFKVDISVIDKLGVHFQEKIAELEKDIYNIAGESFNINSPKQLSVILFDKLG
                     LPIIKKTKTGISTDIEVLERLSSEHAIVDKIIEYRQMVKLNSTYVEGLKNEVNKKTGR
                     VHSIFNQTIASTGRISSTEPNLQNIPTRTEEGRELRKAFVPEDDYILCDADYSQIELR
                     VLSHLANEQNLIDAFEQGLDIHAKTASQVFHVKLSEVTSEMRGRAKAVNFGIVYGISD
                     YGLARDLKIPRKEAKKYIDNYMKYYSNIEKYMKDIVEQGKRDGYVTTYFGRRRYIPEL
                     TSRNFNIRSFGERIALNTPVQGTAADIIKAAMVGVYRRLKNNKMKSRLILQVHDELII
                     EAYKDELEDVKTILSEEMENVVVDFKVRLKSDINTGSSWYEAK"
     gene            20715..21302
                     /locus_tag="PKH42_02150"
     CDS             20715..21302
                     /locus_tag="PKH42_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOA19260.1"
                     /translation="MKLEPKNYNFSNVRYFSPIQLSEHYQLYINYISCLNKVNNDLKN
                     DLIYDECNSNYSDIRSAQKSKTFCFDSIKLHELYFENLTGNNTRIHGPIERVLVNTFG
                     SYNNFREKFKCIGMSMRGWVLLCYDRYNNEFYIYGQDSHDCGVMLYAEPLIVMDVYEH
                     AYMIDFGTNRNVYIDAFFQNIDFNVINQRLNKILK"
     gene            complement(21539..22156)
                     /locus_tag="PKH42_02155"
     CDS             complement(21539..22156)
                     /locus_tag="PKH42_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOA19261.1"
                     /translation="MKNLTINEFAKIVASDSPVPGGGSIAALCGALSAALSEMVANLT
                     VTKKKYADSKEEMNAIINKASQLRDRLLNYIEEDSLAYKKVMEAYKLPKETEEEKCLR
                     LERIQEGLKVAASVPLEVAETSYEIFPLVEAVVLRGNSSSVTDALVGAMMARTGVLSA
                     ILNIRINLDSIKDEDFVKVLKEKADILEGKTRQYEKKILELAPFK"
     gene            complement(22153..23400)
                     /gene="hutI"
                     /locus_tag="PKH42_02160"
     CDS             complement(22153..23400)
                     /gene="hutI"
                     /locus_tag="PKH42_02160"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOA19262.1"
                     /translation="MSKLIIKNASELVTCKGKAPKHGKDMSDIGLIENGCLVIEDDII
                     VDVGTSNILKNYDEDDYQIIDAKDKAVLPGFIDSHTHLIFGGYRADEFSWRLKGDSYM
                     SIMERGGGITSSVRATRNTSLEELTALGKTRLDKILAMGVTTVEGKSGYGLDCETEIR
                     QLKAMKQLNDMHPVDIVPTFLGPHSVLPEYKGKEDEFIQFMIDDVLPKVKEENLAEFA
                     DIFCEKNVFTIEQSRKFLTAAKNSGLKLKIHADEMYQLGGAELAVELGCTSADHLLQA
                     SDEGIRQMSKSDTVATLLPGTAFCLKEEYARARYMIDSGCAVSLATDFNPGSCFTNSI
                     PLIIALAALHMNMTIEEIITSLTINAAAALGRSDTRGSIEKGKKADIIILEYPSIHFL
                     PYHVAVNIVETVIKNGKIVRGLL"
     gene            complement(23475..24365)
                     /gene="ftcD"
                     /locus_tag="PKH42_02165"
     CDS             complement(23475..24365)
                     /gene="ftcD"
                     /locus_tag="PKH42_02165"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOA19263.1"
                     /translation="MDRIIECVPNFSEGRNTEKIEKILDVFRGKKGVKLLDYSSDENH
                     NRTVVTVVGEPEEIKKVMVEAIAKAVELIDLNYHQGQHPRMGAVDVVPFIPVRNVTVE
                     EADKLAKDVAKEAADKYNLPFFLYEKSASAPHRENLANVRKGQFEGMKEKMKDELWKP
                     DFGPDTIHPTAGVTAIGARMPLIAFNVNLSTPDIEVANRIAKQVRHISGGFRYVKAIG
                     VELHDRGLVQVSMNLTDYTKTSIYRVFETIKMEAARYGVNVVGSEIVGLIPMQALVDT
                     AEYYLRLENFKYDQVLETRL"
     gene            complement(24358..26400)
                     /locus_tag="PKH42_02170"
     CDS             complement(24358..26400)
                     /locus_tag="PKH42_02170"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOA19264.1"
                     /translation="MTDNLNIFNAMKIKLSDELPEYPVLKENVRRAPKREVTLNEREI
                     KLALKNALRYVPGSLHEKLAPEFLDELMTYGRIYGYRFMPKERIYGKPIDEYKGKCIE
                     GKAFQVMIDNNLDHEVALYPYELVTYGETGQVCQNWMQYQLIKKYLEALTEEQTLALM
                     SGHPLGLFKSHKNSPRVIISNGLMVGMFDNPQDWAKATAMGCSSYGQMTAGGWMYIGP
                     QGIVHGTFNTLINAGRKELGITDDKDLAGHLFITSGLGGMSGAQAKAVEIANGVGIIA
                     EVDYSRIETRLKQGWVSTVTDNLEEAFEIAREYLYKKQTISIAYHGNIVDLLEYAADN
                     NIHVDLLSDQTSCHVPYDGGYCPKGLTFEERTELLNSNREKFVQMVNKSLADHFKAIK
                     TLVDRGSYFFDYGNAFMKAVFDAGVKEIAKNGNDTSEGFIFPSYVEDIMGPMLFDYGY
                     GPFRWVCLSGKPEDLHKTDQAAMSVIDPNRRGQDRDNYIWIRDAEKNKLVVGTQARIL
                     YQDALGRRDIALKFNDMVRKGEIGPVMLGRDHHDTGGTDSPFRETSNIYDGSNVTADM
                     AVHCFAGNAARGMSLVALHNGGGVGVSKAINGGFGLVLDGSKRVDEIIKTSIPWDTMV
                     GVSRRSWARNEHSIETTIEYNKKFEGEDHITIPFLAKDELIEETLKNYKNKGERNG"
     gene            complement(26540..27451)
                     /locus_tag="PKH42_02175"
     CDS             complement(26540..27451)
                     /locus_tag="PKH42_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOA19265.1"
                     /translation="MLNFKKIELEDKEWIIPILKESVITGCHQNFGNLFAWRDINNTR
                     VANINNFLVAKQLVNGKDVYLYPMGKGDVKPVIEEIIRSADKNLIFAGLSHDEMIELN
                     ELYPGKFIYDQVRNDFDYIYLLEKMVSLKGKKLHRKRNHISAFLRNNPVWSFETITEE
                     NKAECIEMNKKWCIESGCKDDESLMDENCATKYFFKYYDELELEGGLIRVEDKIVAYT
                     IGEVLSNDTYVLHIEKAFKDVQGAYPMINQRFASWIKDKYPHLIYINREEDMGSEGLR
                     KAKLSYYPDKMAIKYRAVTKKNYALHK"
     gene            complement(27557..28249)
                     /locus_tag="PKH42_02180"
     CDS             complement(27557..28249)
                     /locus_tag="PKH42_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOA19266.1"
                     /translation="MLILIVFHLIYINVLFVLYVNLMMGIINIDSLTGGGLIMNLEFA
                     EYSEIFIKYLETWGPAAAIILPVFEAFLPILPLVGFVIINVAAFGFFFGYLYSWIGNC
                     LGSFLLFLLLRKIGGKRINEKIKSSKFRYTLEKVKKKQLNVLFLLYCFPFAPSFLLSG
                     TAALTNMRTEMFLAILLPSKFVMLLSLAFIGENVSSFFENPAKSIFYIIFIVLLNLLV
                     KYLFEKYEQRKQ"
     gene            28382..28882
                     /locus_tag="PKH42_02185"
     CDS             28382..28882
                     /locus_tag="PKH42_02185"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOA19267.1"
                     /translation="MKYFIGAPIPDNYKNKIEMLRAEFRFFTTEPHITLVPPPALPDE
                     DDFIEEVVEVCKKVKPFDIRLNNLDQFGSRVLYVSVDSPGLIDLHNKIYEKLCLQHER
                     RGFTPHLTIVKQRPKRPVDIATIRKRAEIKLIPSPEYTLNSITVYHQPKEHSIYIPYM
                     EIPLGE"
BASE COUNT         8469 a         5669 c         4355 g        10290 t
ORIGIN      
        1 gggataagcg tcggatataa attgacatcc tgactttatc aaaaataaaa aaaattatta
       61 ggaggcgaat tatgaaaaaa gaattgaaaa aaattttagt tttgttgtta gtttcaatgt
      121 ttgttgttgc aagcacaaca attggattcg caattcctgt tacttctgaa aacgccttga
      181 gaatccatga tgatgctata gtgattgata cgcataatga tacaatgacg agagttattg
      241 acagaaatac ttggttgccg gtaaatgata ttggaagttt aacttccgga caattagata
      301 ttccaaaaat ggaagcaggc gggctagatg tggccttctt tggttcatat accggaggct
      361 acacggaaat tggacgtgca ggcagcaggt tgctggcact tattaatgca ctgcactgga
      421 ctgagcgtat gaattcagag aaattcgggt tagcaacgtc ggtaaatgaa atagaaaaat
      481 tggtgcatga aggaaaatca gtaggtgttc caactataga aggagcttat tctcttgaag
      541 aatacaatgc cattgaattg cttcatcaat attatgacct tggaatcaga gtcgttggat
      601 tatgttggag caattccaat tcacttggag aaggtgttaa tgagaggtat gtggatggca
      661 ccccttcaga aggcggatta acataactcg gtgctaaagt agttaaagaa atgaataaaa
      721 tgggtatggt agttgacgta tctcatatga atgaagaaac attttgggat gtcatagaag
      781 ttgcagaggc tcctattatg gcttcacact caaatgttat gggtttgtat tctcatccga
      841 gaaaccttac tgatgaacaa ataattgcaa tagcaaatac aggcggcatt gttcagcaga
      901 atttctatgc cggatatttg ggacctacag gtgacaggga ccttaaaaaa ttagtcgacc
      961 atattgaata tgttattgat ttggtaggca tcgatcatgt cggattaggt tccgattttg
     1021 atggaggagg aatgccggta gatctgccga atgcatcgtt ctattacaga ataaccgaag
     1081 aacttgtgga acgtggttat aaaaagtccg aaatagaaca aatattggga aaaaatactc
     1141 tgagggtaat aaagcaagtt caaaacttag ctgaaaaaaa ccctgataat gtaggtcaag
     1201 gattaacaat tatccctgaa cttgaaatgg gagaaggtgt aagcggtaat acaccgctat
     1261 taaaagctga agtactaaga ctcaatggaa gtaatataga tgagtcaagc ttcagagtaa
     1321 tagttgacgg tatagtttac acacctgaat ttaatgctga aaccggagaa ttaagtttac
     1381 aggttgagga acctttggta gaaaaattcc atgtcgtgac ttttgaagct gtaaataaca
     1441 gcggtaaaat ttcacgcgaa accagaattt tctatattga agaaaattag ctcatagttt
     1501 tgaaaaaaaa ggttactccc tattgaatga attgaagtat ataaaatgga aatagccttg
     1561 tctatttcca ttttttctcc cgtttgtgtc cgtgcaactc caaatttgaa tcttaccgga
     1621 gcaattttaa gattttccct attaattcat attacataat tctattgcca gttttttaat
     1681 gttgtcatgt tttatgtctg atttgtcaga tgtaacttta gaaattttct taactattgc
     1741 cctttcgaag aagttcattt tcctgaagat aaactctcct ccaaagtgtt taaccgatat
     1801 tgctttttcc aaaagctgtt cgggaaagtt ttcagctatt tcctttttaa gctcttcccc
     1861 ttcctgcatg ccgcaaatga ataatcctat ttcctttttc ataagctcat ttaaatttaa
     1921 gtttataaag tctgttactt ccttttgaat tttaccgata taagcagacc ctcctattat
     1981 tacacggtca tattttgcaa gttctatttt gttagtttct tttaagttta cagtatcaac
     2041 catgccatct agttcatttg ataattcaat tgcgcatttc tccgcacaac cgtattttgt
     2101 tccataagca attaatgtat tcattatttt cctccgatta attatttaat gcattttcga
     2161 tagatttcag atatgctttg cagctgacag ttccaccgga tacagcatcg actgtagtat
     2221 tctgtttttc cataactctt ttaaaaagct cgtttcttac atcaggattt acaaacatca
     2281 tatcgtctgt tatttctatt tttgttattc tgttgttttt tacggttatt gtcaattcat
     2341 ttgaccatct gccaaagtca tagcttcctt tataaacgcc atcctcttta agtattaaat
     2401 ccacaggagc aatatttaca tttaatccct cttccaatcc tctgctcaaa tagaacatac
     2461 ttaatacaga aacaactaca aataataaaa acacaattcc taataccttc aatactaatt
     2521 tcattttcta ctccctttat ttaaattttc aatattactt cttcaataaa ataatttatt
     2581 atattttgcc tttgttcttc cccaatattt ttttcaagca ctaattttat tatcaatccc
     2641 atggttgaag ctgcaattat ttgtgctttt aattcgattt gttcatcatc ggcatccctg
     2701 tagatttcct ttatacaatt ataaagagcc cccaatgcag gcttttcttt tgaagctcct
     2761 ttataaagat acgaagtatg cttatgtgct tgaggtgatt ggtttatttg tgctgccaga
     2821 aattcatcag gcatttttaa tgcttcctct atgtagttta ttgtcattct cttaagcctt
     2881 tcccttggtg ttaagctttc tatatcagct gcataaaccg cattaacaat tttcatatag
     2941 cctcttctca ttacaatgtt tacaatttct tctttatctt taaaatagtg atatatgatt
     3001 gaaggtgaat attcaatttt attggctatc tttctaattg acaaattgtc aaagccttcc
     3061 cttgcaatta tttccttggc tgtcgatatg attaattcct tcattgcttc aatttctttt
     3121 tctcttctag taaccatatt acctccttcg ctgaacactg ttctttaatt gaacaccgtt
     3181 cgttaataaa atatcattta gataactgtt tgtcaatact tttttttatt tttttttaaa
     3241 taaaaaaata ctgatacctt agcatcagta ttcataatat taatttctac aaatatctgc
     3301 ccaaccagtt aatgctttct tccatcatat ttgtggtgac aaaatgattt aaattgtgat
     3361 attctattaa tctaattctt tctttgtttt tgtacatgta acttagttta ttataaaata
     3421 tattttggct ttcaatgggc actgaagtgt cactatcacc atgcagcagt aatataggtc
     3481 tgtctgttaa cagttctaaa tgattcatag ggtccaattc tttaaccctt tcttcaactc
     3541 tttcaaattt ctctgtaaca tctgcattcc tgttgaaatt ctcccaccaa cagcagccgt
     3601 ttaacactat aagtgctctt atatccttgt tgtgtgtaaa aattccgccg gcagtaaagc
     3661 cgcccatcga attccccatt attgcaattc tattggagtc tgcagcatgc tttgacacca
     3721 gctcattctt tataatgcta tattcttcaa catttttaaa tacaacgtcc cagaaatatt
     3781 ctatcgctgc ttcccggctg taatcagcca acggtatcct ttcaccgtga taaatggcat
     3841 ccggaattac tacctgatac ccgactgagg ctaatataaa tcctctcatg cgctgagttt
     3901 ctttatttga actccagcca tggtagaata ctactgtagg tattggctcc ttagcatctc
     3961 tcggtctaaa aataattgcc gggatttctt tcagcttaac tttttcctct attatgtaat
     4021 ttgattttat aaaatcagcc ataaattctc cttgtatatt atattgtccc gcaaaattct
     4081 ttcactagaa aaagatgcag catattctat tcatcatcga aataattaaa ctcattatta
     4141 ttatttgcat agaattttga ttcattataa taaatattta atttctttaa atttgcagtc
     4201 cacaaataga ttacaaacgg tataaacaaa aacatcagcg gttgaatgtt tgacattaat
     4261 ataaaatcga aaattccgtg cataatagcc ggtaccgtta atgatttttt gaagaagtaa
     4321 cggtgttctt ccggtgttga ggaaaactta gccagtgaaa gataataccc cattgttaca
     4381 gcaaacaaca tgtgtgccgg aaccgaaata attgctctgt aataacccac tgattcaacg
     4441 tccgagaaca ggaatacaac atacattata ttttcaaaag ttgcaaaacc cagggctgac
     4501 attacgcagt atactattcc atccagcttt tcattaaaaa caggattaaa atacacatac
     4561 ttcagcacaa cttttctttt tatgtattct tccgttaaac cggcaacaat aaatgccgtg
     4621 taggcagctc ccaaaatacc tccaaatata ttgaagaata ataagaagtt ctcaataagt
     4681 attgccggaa taacataaag agctccaaga acaaacacct ttaataacat gcttataggc
     4741 tctctatcgt gtctgtcaaa ccaataaaaa gtcaatgcca atgctatgcc cggagttatt
     4801 gctgtaacaa ataaatttaa tttcacaaca agatttacct ccactcttct tcattaaatc
     4861 caactatatc atactttgta aactatcagt atccatatct tgagctatta atgtgatttt
     4921 aaggactttc cctaagaatt gaattaatgg gataaaaact tatcaatacc attttaattg
     4981 ttatatatta tatagcaaaa tttaacaatc ttcaacattt atattattgt tataattgag
     5041 aaaacaaaaa tctcccgaat taacttcggg aaatctttgt ttataaatat aatttgctca
     5101 ttattgttag ttcatgctat gattatattt aattcaatga cttaatctcc tctttaagtt
     5161 caagccgcag ttgttccatc tgtttgcgcc acaaaacaag atcctgtgcc tttgcttttt
     5221 taccgtttaa gataacagcc ttacaatttt gaatcacagg cagctctgct gccgctattt
     5281 tgcggcatgc ttctactaaa tagtatagtg ttccttccgg tatgataagg gcaccatatt
     5341 gatcacaaaa tattatatct cccggataaa ttttggttcc aaaaatatta acggaacaat
     5401 cgaaatcaac caagcgaata ttagcatggg aaaccatttt acagcttgcg taaaaagctt
     5461 tttttaatat acatgatatt tcatatacct ttctataagc attgtaatat ttcaatggtc
     5521 ttgtattgga gttaatagca agaacttcgt cactttctag aaatagatct agtttgttcg
     5581 catcaactac tacctttgtt tttaatccat gctttcgaaa ctgtatacct gtatcgcttg
     5641 cacctgtgag atcatctgct attatcgcta tagaacgcat ttattggctc ctttcagtat
     5701 gtcttttaga tttcatttct ataatgtgtt atagtttgta attcatctca caattaatat
     5761 gtatgatact tacaaatctt ttattgttat gaaacaaatg gacgtcaggt tgcgcaagaa
     5821 ttgtcactct gagcaaaacg aacaagtctc aaacgtctct aaatgcagat tctacactgc
     5881 gttcagaatg tcaaaagtgt cttgcgcagt ctgacgtgtg ggggactgtc atttaacaaa
     5941 tatcttttat ttactaataa acttgcaaaa cctatgccat attttagata taaaaagcaa
     6001 ctgccataaa aatgagcaat tgcttattca aattgaattt attcttttta tattcattac
     6061 ttccgaaaca aataaattaa ttatttttca atatgaaatt aacaaattct atatatatat
     6121 gttattacag ctaacagcac tccacaatac acatatttca ttatgaaata aatttcaatc
     6181 tgacatattc cattttttaa gtcttcgcca taatgtggtc tgacttatac caagtaaatt
     6241 acaagccttc tctttgtctt gatttgtaag gtttaaggta atggaaataa tctccttctc
     6301 catatcctct aaagtacctt ttaattctac ttttttgtct ttataaactt tttcgctcag
     6361 tatcttttga ttttccagtt ttctcatttc atcgatatcc tctagttgaa ctactgcacc
     6421 atcttttaaa gctacaatcc tttcaagaat attttcaagc tcccttacat tccctggcca
     6481 atcgtaggag caaaatttac ttagtacctt atcactaaag attggtctag gtttatatgt
     6541 ccttttacat attttcttaa gcaattcatc tattaataaa ggtatatcat ccaacctttc
     6601 ccgcaaaggg ggcaatgaca tcctaagtac attcaaccta taatacaagt cctgtcgaaa
     6661 cagacctttg gatacagatt cctctaaatt gcgatgagta gcagcgataa ttcgaacatt
     6721 aaccggtatt actgtgtctc ctcccactct aataatggct ttttcctgaa gaactcgtaa
     6781 taaacgtgcc tgtaatttaa ttgaaagttc accgatttca tccagaaata aagtaccttc
     6841 atgagccaac tcaaataacc cttttttacc tcctcgtctt gcccccgtaa aagcaccttc
     6901 ttcataccca aataattcac tttccaaaag gttctcagga acggaagcac aattaatagc
     6961 aacaaataac ccatttcttc gattactgtc attatgtata ctttgtgcaa acatttcttt
     7021 tcctgtaccg gtctcccctg taatcactac cgttgtatca gccttggaaa attgcttagc
     7081 ttgcttaata acttttttca taataggact atccgttaaa atatcttcaa aagtatattg
     7141 cgcaacatgt ccttttaaat agagctccct ccttactctt gtttcaatgg cttgaaaatg
     7201 acttgcttct tgaaaagtta ttactgttcc ttcaattacg ttatttatat ttaaaggaat
     7261 taaattatgg actatcatcc tattgcccac cctgtgaggt tcacccaaca atggtttgcc
     7321 cttttcttgt actctcccta aggcaatgct cggcaataca tgatcagctg tttgtccaat
     7381 cattgcagtt ttatttttac ctaagatttt ttctgcggca ggatttacca atgaaacaat
     7441 accctcagaa tcggtcgcta taatcccttc atatgtaaag tctaatattg ccttaaactg
     7501 ttcagccctt gtcttttctt ttcgacgaac tgatgccagt tcttgcgcct ctcttaatgc
     7561 catacttacg gattctttac ccgagtacac aagaattgat ttcaatcctt gttttttgct
     7621 atagcgaaca gaaaccgcat caccaactat tacctgtata ttatcttttt ttgccttttc
     7681 aataacatcc aatacttcgg attcgtcttt tatttcaagc tctttaattc tgacgtctaa
     7741 aatttcaccc agactttcac acccatatat aacattttta aatcctatta ttccaataga
     7801 atttccaatc ttttttgcac ttgcaacagc ccttattata tcatagggac ttaccatgat
     7861 ttctatgact ggttcaccca ctttttccat aatagccgta gcagtaccgc ctctgcttat
     7921 aattatttct gcgccgcttt taacaaattt ctttgctaat tttacccctt cctccaaatc
     7981 accaacagct atatcagctt ttatttcaag ttcatcacat acctgttttg ccatttccgc
     8041 caattctgga tagggtgcaa ttatagctaa ttctgatgac aatttaacat ctccctttct
     8101 tcagttaaaa atctaaaaat ggattatatc cttcgtttca attagaatga taataaggat
     8161 aatttatatc tttatcaaat atcctccaag caaagttgct gtgtccggac tgcaggttgt
     8221 aaatattact tgatgtttat ttgcaaaccg gctgatgagt ttcacggctg tgtcttttct
     8281 gtttgggtct aagtctacca aacaatcatc aagtattaaa aatcccttgt tgtctcccaa
     8341 tatatactct gcaatggcaa gtcttaaggc taatgcaaca caatcgtatg ttcctgaaga
     8401 caaaagacta aggggcattt ctacctcgtt ttcatttttt agctttaaat taaaatcatc
     8461 gtctatatct gtggctttat agctgccgtt tgtaagcaaa ttcaagtaat tggaaaaagc
     8521 agtaattacc ggaataaatg attgctcatc catttttagc ttgatgtttt caaagttttc
     8581 cttaatcttt aacagtcttt tccctttttc cagtcttttt tcaaacagtc tttctgcttg
     8641 tttcagcgac tcagacagtt cttcatatgt tgattccggt aattcttttt caagccggta
     8701 atattcttct tttagacttg ccaaagtatt tattaaataa ccgtatttat tcctgatttc
     8761 agttaaaatt cttctgaatt catctgtgga tttatattcg ggtggtagct ctgccaactt
     8821 atccaaattt gttttaattc cgttttgttt taattttatc tctatgattt tattaaacaa
     8881 accgtctaaa tctttatatt cttctgccca tttgtcaata ttgctctcca gcaatttctt
     8941 ttctgaaatc agctctattt tttcggcatt tatggaagtc atttcggact caacaatagc
     9001 agtgtttcga acaagcgaca agtctcccaa ttctcttatc ctgtctttta attcttcata
     9061 ggttaattct ccaagatatt ggtttttctg cataatgcat gattccaaag tcctgtttaa
     9121 gctgtccaat ttttccttgt tgagttttgc ttcttcaaga ttttttactt ccagtgtttt
     9181 taaaagctgc tcgtaatttt tcttaaaatt ctcatattga attcttattt cattaaaatc
     9241 gatgtttcct gatttaattt ctatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnttgtca gttctgttat aagctgtttc aatttttctt cgcttcgagg ccaatcccgg
     9421 tttatctttg aaagggcgga tatatcttta ttaagctgct caatttgagg ctcaaggata
     9481 gacctttgga ttacatcgtt ttctaattcc tccatggatt ctttcttggc ctttaattct
     9541 tcgatttttt cttcggtctt tttaatctgt acgcagattt cgttgaattt cttttcagcc
     9601 tcttctgcct tagccatttc cagttttatt ctttctttgg catagtagct ctccaccact
     9661 ttaccaaaac ccactttgta cggattgttt atgcccttgt tattttcagg atagtttctt
     9721 tcaatgtccc atctctttaa taaggtgtca atttcatctt gaattttttg tcccaagctt
     9781 tcaagagata ctccgtcaag ctccataatt gtgcttctta acaatgagct tatttcacct
     9841 gttgcttctt tattctcaac tatcctgtta attgcttcct taagatagta ttgctttaaa
     9901 aagaacacgc tgctatatgt cccttcaccg taaatgtata cgtcctttaa ggaatcattg
     9961 atacttatgt cgctttttat tacaatttta ttgggcatag tcaattttga agaagcgtct
    10021 gctccccatt ctttccttaa tgtataaatt tctccattat gctctaagtc tatttctccg
    10081 tctatgctgt cccctatggg ataaggcata aatttagagt gaaactcttt atcctctgct
    10141 gttttcctgc caattctgct cggcttgaaa agcactgaat gaattccttc aaccaaagtg
    10201 gattttcctg actcgtttga gccaagaatt acattgagcc cgtctttaaa ctcgattttt
    10261 acatccttaa tgcctgcaaa tcttccggtg gtatattttt taattatcat ttcttacctc
    10321 cataataagc tcataagcaa gctgcaaggt gtcttcgtca tctgaaagtg acaaaaggag
    10381 ttcctgagga aatgaaccgt ctgaaaattc tctgtgtatt ttttcagttg ttatcttcat
    10441 tttaaaatcc gaatcctcga ctattaagta tattatattc ttttcaatcg agccgtaaat
    10501 ttcctgccta tactggaaca cgtcttcatc aacactgcct tctaaggtta ttcgcgctat
    10561 ggttttttcc ggttcatcct ttataagctc ttcaagtatt ttatctaagt catctctgtc
    10621 ttctatcgtg taaactttat ctttaaattt atatgtgcct gtttgtatta acttggcatt
    10681 tgttttttta ttgtcatcaa caataataat ccaagcttgt ccattgtgtt tgcagtccaa
    10741 tccgtcaggc tcgggggttc ccggattaaa tattttttca ttggtaatcg gtgatttaaa
    10801 cgggtatgta atatgtgtgt gccctaatag ccacaggtcc ataggaatat tattaagctc
    10861 tttaatcgac atattgtagt agttcttatc caaatccggg gacaaacctt caagagcccc
    10921 gtgtgcgatg cctatattta cataattatc atcaatttct ttttctttaa tccactgaag
    10981 gttattggtg cttgaatgct ttgaatggca tggagcagga aatataacgg cattaagacc
    11041 gtaatcttct aaagaatagg cgcactcttc gtttataata acaatcttat cagaagcagc
    11101 tttattaaag ctatcccaca ggtcaatcat accgttatcg taatcatgat tgccgggtat
    11161 aaccaacaca cattctcctt gaaaattatt taatgcagtt ataactcggt ccttggtttt
    11221 tttatctatt ccggttatgt tatcaaacaa atctcctgca atgacaaaca ggttgcattt
    11281 ttcctcgttt gcaaatttta tcatgttatc taagacgtct aatcttgctt ccttaaggta
    11341 gccttttaca ggttcaggat aattattgaa cttcattccg atatgtacat cagctgtatg
    11401 aaaaatttta atcatttttt cacctcatta atatttcggg gatgttttgg ggatgcttct
    11461 tttgttctca tagtgacaaa gtacgaggta tgttctatgt tgttaatatt atatagcaga
    11521 atactgcaat tatcaacaag cagaaaataa aaagtccttc ctgaaatatt ggtgccttat
    11581 tggtgcctgg caccaaatac atggcactaa aaagtaggtg ccaagcacct ggttggcttt
    11641 gatgcttggc acctacattc ttattcacct attttataag tgtaaggaac catttgattg
    11701 gaacaagagc cattgccttt aatgcggcga ttttattaat taggaattta cttttttctt
    11761 caaaaagcaa taccctcatg tactcttcat taactttctc actttgacaa aaaactttta
    11821 agttgtaatt catgtcttta ttataattat agtcatactt atacatatca gtcctcacta
    11881 ctgccaatac atttccaaca attcattttt catatgtgcc tttctcacaa taaagttaat
    11941 ttcaatagag caaactaacc ttaggtaaat tatggaggct accctatttt ttattaagca
    12001 cattaacttt gcatttctga cctgaccata aagtttctgt tacttcaaag ttaatatccg
    12061 gatttaagcc tctaatcata ccttccatcg ggtctaaaca gaagatattg catggacttt
    12121 gagctccaat tttttttgcc aatgcacata gcatgcactt tgttgcttca gcctttaagc
    12181 cttcattatc gctgttaacc gaccaatttg cacaaccgaa taattctgat agcactaaaa
    12241 atacatcttc cggtttatca attccaaatt gcgatgcacg atattttccg gtgtgcattt
    12301 gttcttctct cttttctaag gctacctttt caagtattcc ttctttcccc atcctcaaaa
    12361 cagagtctgc caatgctcct gcataaatta cctgaagcat tttaattttt tgttctaatt
    12421 ccattttaaa cctccaaatt ttttaataaa aaaaccttcc atatttgttt tcaatacatt
    12481 agtggaaggt tttttgtttt tgtgacaact aaatttattt taataaataa tctttcgtaa
    12541 gtattatttc agaagccctg taaaaattta ttctatttac tgtctgcctt aattttttat
    12601 attcctcatc tagttgtatt tccttaaccc atttactcat tctgcatttg cactttgaat
    12661 caggattttt taatatgcac tcatcattta aaaagttacg caatttatta cgtgaacgag
    12721 ataccatttt tcttatgctg gcttcatctt tttcaaaaat tgctgcaata tcggaataag
    12781 gtaacatatt aatatcgcgt aaaatatatg ctaatcttga ttcattatcc aagcaatgca
    12841 aagttcccgt caggcattta tcacacatct ctcttaccca taactgtttg tcaaaatcct
    12901 cataatcggg tatttctata tcctccccgt gaaaatagtt gctgagaaaa tatgtagaat
    12961 atttatgttc ttcctttgcc attttcaaaa caatccttga agtaatcgta tatatccaag
    13021 tggaaatttt ggattcgcca cgaaatgtct taagactttt attaacctcc aaccaaacct
    13081 cttgggcggc atcgtctgca gtatctttgt tttgtatcat ccttctgcat attgaataga
    13141 ccatctttcc ataattttcg atgatgtatt caattgtcaa attattattt ataacacttt
    13201 ccatacatac ctctcgtatt tatataaaaa ctcttttatg caatctttcc catattataa
    13261 cacattaaaa aagattttac attaaataaa aaaattctat cctgtcctca accatcctta
    13321 ggtgacctca aaaaattgta ttgctgataa taaaaaatac ctgttataat gattacgaca
    13381 agggtataga gtaagtataa attaatcgat tataataatg gaaaggttac tatgattaag
    13441 aaattgattt tactgttttt tttgttaata ttggtaattg ttttctttgc ttgtgataat
    13501 aataagaaga gttacaatat gacagcaaat aatgataatt taagaaagga tgatgaactt
    13561 atggataaag aattaaaggg caataaattg atttaccttg caggaggatg tttttgggga
    13621 acagaaaaat atttaggctt aatcaacgga gtagttaaga ctgaggtagg atatgcaaac
    13681 ggaaatacag aaaatccttc ttatgaagat gtctgcagga gaaataccgg tcatgcggaa
    13741 actgttaaag tggtttataa cccgaaagtg attgatttag aaactttact taagttattc
    13801 tacaaagcaa ttgacccggt agctataaac agacaaggct atgatatagg gactcaatat
    13861 cgaacaggcg tgtattatgt tgacaaagag gatgaagctg taattaaaga atcattgaat
    13921 gaattgcaaa aggaatataa ggagcccttg gctgttgaag tgacggaact taagaattat
    13981 tacaaagcag aagaatatca ccaaaaatat ttagaaaaga atcctggcgg atactgccat
    14041 ataggtgaat tggaatttta cgagttggag aaagctcaaa aaaattcaaa gtataaggta
    14101 aaaccaaaaa aggaattaga gtcaacactg acggaaatgc agtacaatgt tactcaaaac
    14161 aatgctactg agccgccttt tgataatgaa tattacgata attttgaaga tggtatatat
    14221 gtggatataa cttccggtga acctttgttt acatctaagg ataaatttga ttcaggatgt
    14281 gggtggccaa gtttctcaaa gcccattgat gaaaaagttg ttaaagagaa aagcgattta
    14341 agttacggca tgataagaac agaagtaaga acgtcaaaca gtgattcgca tttagggcat
    14401 gtttttaatg atggtcccaa agaatcgggc ggattaagat actgtatcaa cagtgctgca
    14461 ttggaattca ttcctgtaga taaaatggaa gaaagaggat atggagactt gctttattta
    14521 tttgaataaa taaattcgct gtgtcatcct gagcctaagc gaaggatctc aggttttata
    14581 aaatgagatt cttcgagcta atgctctcaa aatgacacgg gcttatagat agcaatgaca
    14641 aaaatgttaa aatattgtat tgcatattat gacaaaattt tatatgataa catacatatc
    14701 ataataaatt ttgcagctat aaatggaggg taaaaataaa ttgactaaaa caaaagaagt
    14761 tatattatgc ggattattac tggcaacagg tataatacta cccatgtttt ttcatatgtt
    14821 tggtttgaca ggtcctattg ctttaccgat gcatatacct gtactaatcg gaggtttttt
    14881 attgtcgcca aagcttgcat tgctcttagg tataataaca ccggtcttga gcggaatgtt
    14941 aacaggaatg cctgtaatgt tccctatggc tgtaataatg gctttcgaat taggaatata
    15001 tggtttagca gcatcattag ctgtaagaaa atttaactta tcagttatac cttcattaat
    15061 tgtttccatg atagcaggaa gaatagcagc cggtttaaca gttgcaatct tagttgaatt
    15121 attcggagta aaaatgaacc ccctcattta tatcaagggg gcaataataa caggtattcc
    15181 cggtatcata atacaattaa tctttatacc ggcattagtt tatgccataa aatcttatgt
    15241 gaaaattaaa tcggtctagt tggaaatcat cctttcaatc cattccttca aaacaatggc
    15301 atgattgaat tcttcatttt tggcagcata caacaatgta acatttcctt cttttaattt
    15361 gtcctttaat aaattgataa aatcaagggc atgttcattg ttatccagct caaaattata
    15421 tcttaccctg aattcatcca ttgtatccgg gtcatgttta aacgtcttcc ttagctcagt
    15481 gctgggagtg atttcctttg cccatttatg aatatttatt ttttctttgc ttattcctct
    15541 tggccaaaga cggtctacca atattctgta accgtcatct tcagaggagt cttgatatac
    15601 tcttttaatc ttaatattat acatatcttt tctcctttta cttcattatt cattggtgcc
    15661 tggcaccttt ggcttattgg cgcctggcac cttcgtatgg cgtcttcata tttataatga
    15721 caatggatga aaatattatg ataattccaa gcatatttat aaaatttaat ccttcttcga
    15781 acacaataat tccaatcagt gtagcaacca ctatctctac tgatgcaatt acaggagtct
    15841 ttgagatttc tatttgcttt ctcaaccctc ccaagtataa caaatatgac cctacggttg
    15901 gtattagacc atacccgaac gaatagatcc aaaatttcag tccggatacg gttttaatag
    15961 actcccaagg tgaagtaaat attccaatgg caattccgcc aaataaaaag ccgtaaaata
    16021 caaccgtaaa aggatgaata tcacccgagg tagactttcc tattatagtt accaagctat
    16081 acaaaaaacc tgctgctacg ccaaaaaata ttccggcggc cgataatttt atatttgaca
    16141 gattcccgcc cgtaaccatt aaaaaacatc ctacaatatt tataattaat gcaagaattt
    16201 taattcttcc tattgattcc ttgaataaca ttcttgacat gatggcgaca aaaacaggtg
    16261 ccgtatataa aagtatggct gcagtggcaa ttcctacgct tcgaatgctt gtgatataac
    16321 tgtaattaaa aagagcctgg gacaatatgc ctaacataaa gcattggctc aacccctttt
    16381 tgtcaatttt aaacaagctt acgccgccct tgtgaagcat tatggggatg agtatcaaac
    16441 atcccataaa catcctcata aatgccgtta atgtggtgtc agcgccggca ctgtttaata
    16501 aattaacaaa cagtcctatt gtaccccaca agcaacctgc tgcaaaaatc atcaaatagc
    16561 cgatattgtc gcgcgtaaaa attttcctct taaattgaat tattttattc attggtcagt
    16621 ctccggtatt tgtgtcccgg aagtgtctag accggtattt gccatactat taatgatatt
    16681 tctgaattca tctgttacgc tgtaaccttg gaatttgact ttgttatatt ttttgctgaa
    16741 ttcatcgtag tatttttctt ttacatatac agtcttgttt tcaatatcat aatacaaatc
    16801 aaagttgtat tcttcttcta cactgaaaat caatactttg tcttttgtaa aaatatcagc
    16861 ttctatccaa taatagccgt catcctttgg gggcagttca gctgttactt ctgttttttc
    16921 ggagataagc agattttcaa gcaaaatttt tacgtcgccg gccttgtcca tatcttctga
    16981 cagacttccg tcgaaagtca taatcctttt tatattttca tgctgattgg tatatttaat
    17041 tccttcattt tcataaatag ttgcatcaat tttggaaacg gtgctgattg aaccggcaat
    17101 tcgagtaata tctttctcca atgcttcttc aacctttgaa atatatctgt ttttactgaa
    17161 tattttcatt actagcagta taataacaac aagtaccgca attaaaaaag caactcgtcc
    17221 gtaattaatt cttcttcttc tgctgtacac tctttttctc ctggccattt caccacccca
    17281 tacctcttat ttcaagcaga gcttttatat tctcctttag ctcttcaaca gtgccttcgt
    17341 tatttataac tacatcggcc ataaatactt tttctttaat agatatttgc ttatttataa
    17401 tttttagtac atcttcgggt tttttgtttt ctgcaatggc tctttccata agtctttttc
    17461 tttgagtttc ctcggaaaca tatatcaacc atatttcgtc gtatatgggc tcatgtatat
    17521 ctttggtttc aaatagcagt ggtatgtcta gaaatattac cttttctttg cttgcctcaa
    17581 taccttcttt taatttgtca tatacatagc cgtgcaccat attattaagt tcatttaatt
    17641 ttttatcgtt gttaaaaacg atttctccaa gcttttgcct gtctatttca tcacctttta
    17701 atatatcctc gccaaaatga ttgataattt tgtaatagag ctcacttcca atattgtatc
    17761 cttcatggac aattttatcc gaatcaagaa ccgtgaaccc aaactcctta attatatcaa
    17821 cagctgtaga ttttcctgtt ccaatacttc cggtaatcac tatgacctta ggtctatttt
    17881 gcttcatacc agctactccc cgtattaata tcagacttta agcgaacctt aaaatcaaca
    17941 actacatttt ccatttcttc tgaaagaatt gtctttacat cttcaagttc atctttgtat
    18001 gcttcaataa taagttcgtc atgaacctgc aggataagcc tcgacttcat cttgttattt
    18061 ttaagccttc ggtaaactcc gaccatggct gcctttataa tatcagcagc ggttccttgg
    18121 acgggagtgt ttagagcaat tctttctcca aaggagcgta tgttgaaatt acgtgatgta
    18181 agctcgggaa tataccttct ccttccaaaa tatgtagtta cataaccgtc ccttttaccc
    18241 tgctccacaa tatccttcat gtatttttct atatttgaat agtatttcat ataattatct
    18301 atatattttt ttgcttcttt tctcgggatt tttaaatctc tggcaagtcc gtaatcactt
    18361 atgccgtaca ctattccaaa gtttacggct ttggctcttc ctctcatctc tgaagttact
    18421 tcggataatt taacatggaa aacctgagag gcagtctttg catgaatatc taacccttgc
    18481 tcgaatgcat ctattaaatt ctgctcattt gccaagtggg acagtactct aagctcaatt
    18541 tgcgagtagt cggcatcgca aagtatataa tcatcctccg gcacaaatgc ttttctcagc
    18601 tctcttcctt cttcggtgcg ggtgggaata ttctgaaggt ttggctctgt acttgatatt
    18661 cttccggttg aagcaattgt ttgattaaat attgaatgca ccctgcccgt tttcttgttc
    18721 acttcgtttt tcaatccttc cacataagtg gagtttagtt taaccatttg cctgtattca
    18781 ataattttat caactattgc atgctctgag ctcagtcttt caagcacttc aatatcggtt
    18841 gaaataccgg tctttgtttt ctttattatc ggcagaccta atttatcaaa caaaataacg
    18901 gaaagttgtt ttggagaatt aatattgaag ctctcacctg caatattata aatatccttt
    18961 tcaagctccg cgattttttc ctgaaaatga actcctaatt tatcaataac gcttatatcg
    19021 actttaaagc ccacaaattc catgtaagcc aaaacttcaa tcaatggaag ctcaatttct
    19081 tcataaagac aggtcatctt aagctcgtct atatcctcgg ttatttgttt ttgtactttg
    19141 cctatggtgt ttaagtaatt gaatatatat gccttccttt gttctatgtc aatttccttg
    19201 aatgtttttc ttttaactcc cgtaccaaga atttgatttt ctgaaggaat ttccacattc
    19261 aagaattcgt aggcaatctt gtccaatgaa taggagcttt ctgaagggtt caaaaggtat
    19321 tttcctattt ccgaatcaaa ttggatatta tttatttgaa tattgttttt cattaaatag
    19381 ataatttcat tttttaaatt atgtccccaa attgcgattt tttcgctttc aaatatttct
    19441 tttaattcat taagaacttt tttttcttct gctttttcaa aatctatata ataaatattt
    19501 tcaccgtcgg aaatacctaa ggcaagagca ttgctaaaca gtgctctttc tccgtcaagc
    19561 aaaaatttta atatcgcttt ctctttgcgg cttatatttt tgattatttc tgttaatttt
    19621 tcttctgagt caatataaat aatgtcaata tttttaactg tttcagccct gtcggtatca
    19681 aacaatgata tttgcccatt atcatcaaca ggaatagttt caacaagacg ctttttaaac
    19741 tggcggaatt ccagttcatc atataattct gacagtttct taatatcagg tttcttaatt
    19801 ttgaattcct ctatattaaa ttcaatcgga atatccgtaa taatacgcgc caaggtctgg
    19861 ctcatataag cctgcatttt atccgtttcc agtttttctt taagcttgcc tgtaatcttg
    19921 ttaatgtttt catataaatt atctaagctt ttatactcct ggataagctt aagagctgtc
    19981 ttttcgccta ctcccgatac accgggtata ttgtcggagg aatctcccat gagagccttt
    20041 aaatcaacaa attgttcagg ttttaacccg tactcttctc ccatggagtt tatatccatt
    20101 tccacaatat ttgtaatacc ttttttagtt aaaattactc ttacattttc atctatgagc
    20161 tgcaggtagt ccttgtcact ggtaaccagg taaacatctg ctccctgctc ctttgcagcg
    20221 catgcaaatg taccggatac atcatctgct tcgaagcctt caacctctac cctttttata
    20281 ttatgtacat caagaacatc ccttataagt ttaaactgct gaaccaattc atttggtgct
    20341 ttttgacgat ttgctttgta atctttatat ttttcgtgcc tgaatgtcgg tttttcaggg
    20401 tcaaaagcaa cacataaata ttcaggggaa tatgtatcta taattttata caacatgctc
    20461 aaaaaaccgt atacagcatt tgtatattgt cctttttttg tctttagggg cggtaatgcg
    20521 taaaatgctc tgaacagcag gctgttgccg tccaatatca ttattttttt catggcatct
    20581 cctttatgaa ttgacccgat ttcttgtgtt ttctttatta tatcaacaaa aaccatgcgg
    20641 gtaaaggaaa actttcagtt catatattca ttttcccgga catataatta aatatattta
    20701 tcggaggtat aaaaatgaaa ctggagccaa aaaattataa cttttcaaat gtaagatatt
    20761 tttcgccgat tcaattaagc gagcattatc agctatacat aaattacatc tcttgcctta
    20821 acaaggtaaa caacgatttg aaaaatgatc taatatatga tgaatgcaat tccaactaca
    20881 gcgacattcg ctctgcccaa aaatctaaaa ccttttgttt tgacagcata aagcttcatg
    20941 agttatattt tgaaaattta acagggaaca ataccagaat tcacggtcct atcgaaagag
    21001 tactggttaa tactttcggt tcctacaata atttccgcga aaagttcaaa tgcatcggta
    21061 tgagtatgag aggatgggtt ttgctttgtt atgacaggta taacaatgaa ttctatatat
    21121 acggtcaaga ctcccatgat tgcggtgtaa tgctttatgc agagccttta atagtaatgg
    21181 acgtttatga gcatgcttat atgattgatt ttggaacaaa cagaaatgta tatattgatg
    21241 cttttttcca aaacattgat tttaatgtaa ttaatcaacg gttaaataaa attttgaaat
    21301 aatcacagta actaaagaaa ccttcagaca tataatatca tacacacata aatttaagga
    21361 ggttaattat gggatttttt catagaggta gagtagccgg agcaggcggt tcaaatttac
    21421 tatttttctt cttattatta gttatactgt ttgacgagtg ctttgatgac atgaagtgct
    21481 agtaaccctc gggacagcta cataagctgt cccgttctag ttggtgcata ctgtgtcatt
    21541 atttgaatgg cgctaattct aatatttttt tctcatattg tcttgttttt ccttcaagta
    21601 tatctgcctt ttcttttaat acttttacaa agtcttcgtc cttaattgaa tccaaattaa
    21661 ttctaatatt taatatggct gacagcactc cggttcttgc catcattgca ccaaccaagg
    21721 catccgtaac agaacttgaa tttcctctta acactactgc ttccaccaaa ggaaatattt
    21781 catatgaagt ttccgcaacc tctaatggta ccgatgctgc aacttttaat ccttcttgaa
    21841 ttctctcgag gcgaaggcat ttttcttctt ctgtttcttt tggaagcttg tatgcttcca
    21901 taaccttctt gtatgcaagg ctgtcttctt cgatatagtt tagaagccta tctctaagct
    21961 gagaagcttt attgattata gcattcatct cttcttttga atcagcatac tttttctttg
    22021 taacagttaa atttgcaacc atttcggaaa gtgcagcgct taatgctccg cacaatgccg
    22081 caatacttcc tcctccgggc acgggtgaat cagaagcaac tatttttgca aattcgttta
    22141 ttgtcaaatt tttcataata accctcttac gattttaccg ttttttatta cagtttcaac
    22201 aatatttact gcaacgtggt atggaagaaa gtgaattgaa ggatattcca atataataat
    22261 atccgccttc ttaccctttt ctatacttcc tcttgtatca cttctgccta atgcagctgc
    22321 agcatttata gtcagtgaag ttataatttc ttcaattgtc atattcatat gaagagcagc
    22381 aagagctata ataagcggaa tggagtttgt aaaacagctc cccgggttga agtccgtagc
    22441 caaagaaacg gcacatcccg aatcaatcat gtatcttgct cttgcatatt cttccttaag
    22501 acaaaatgcc gttcccggaa gaagggttgc aactgtatca gacttagaca tctgtcttat
    22561 tccttcatct gaagcctgca gcaaatggtc tgccgaagtg cagcctaact caacggctaa
    22621 ttcagctcct cccaactgat acatttcatc tgcgtgaatt tttaatttta accctgaatt
    22681 ctttgcggca gtcaggaatt ttcgggactg ctctattgta aatacatttt tctcgcaaaa
    22741 tatatctgca aattcagcta agttttcttc cttaacctta ggcaggacat catctatcat
    22801 aaattgaata aattcatctt ccttgccctt atattccgga agaacgctgt gaggtcccaa
    22861 aaatgtagga acaatatcaa caggatgcat atcgtttaat tgtttcattg ctttaagctg
    22921 tcttatttcc gtttcacaat caagtccgta tccgcttttt ccctccaccg ttgtaacgcc
    22981 catagctaat attttatcaa gtctggtttt ccccagagct gttaattctt ctaaggaggt
    23041 gttccttgta gctcttacgg agcttgttat tcctcctccg cgctccatta ttgacatata
    23101 gctgtcgccc tttaatctcc atgaaaattc atcggctctg tagcctccga aaattaaatg
    23161 tgtgtgggaa tctataaatc cgggcaatac ggctttatct tttgcatcaa taatttgata
    23221 gtcatcttca tcataatttt ttaatatgtt cgaggttcca acatcaacta ttatatcgtc
    23281 ttctattaca aggcatccgt tttcaatcag ccctatatca gacatatcct tgccgtgttt
    23341 gggggctttt cccttacatg taacaagctc ggatgcattt tttataatca atttactcat
    23401 tattacctcc tttatgacaa ggggacgggg gtgttgtcat caaatgtcca ccaagaaccg
    23461 tcccccctgg tcatttataa ccgggtttcg agaacttggt cgtatttaaa gttttccagg
    23521 cgcagatagt attctgccgt atccaccaag gcctgcattg gaatcaaccc tacaatttcg
    23581 cttccgacaa catttacacc ataccttgcc gcttccattt tgatagtttc aaatacacgg
    23641 tatatgcttg tcttggtata atcggtcaag ttcattgata cttgtacaag acctctgtca
    23701 tgtaattcaa cgcctatagc ttttacatat ctaaatcctc cgcttatatg tcttacctgc
    23761 tttgcaattc tatttgcaac ttctatatca ggagttgata aattaacatt gaaagcgatc
    23821 agaggcattc ttgcaccaat tgccgttacg cctgcagtcg gatggatagt atcaggtcca
    23881 aagtccggct tccaaagctc atccttcatt ttttctttca ttccttcaaa ctgtcctttt
    23941 ctcacattgg caagattctc tctgtggggt gctgatgcag acttctcata aaggaaaaag
    24001 ggaagattat atttatcggc tgcttcctta gctacatcct ttgctagttt atcggcttct
    24061 tcaacagtaa catttcttac ggggatgaaa ggaaccacat ctaccgctcc cattcgggga
    24121 tgctgtccct ggtgataatt caggtcaatt agctctactg ccttggcgat agcttcaacc
    24181 ataacttttt taatctcctc aggctctccc acaacggtta ccactgtacg gttgtggttt
    24241 tcatcgcttg aatagtcaag aagcttgact cctttttttc ctctgaaaac atccagaatt
    24301 ttttctattt tttctgtatt tctgccctca ctgaaattag ggacacattc aattattcta
    24361 tccatttcgc tctcccttat ttttgtaatt ttttaaggtt tcttcaatta attcatcttt
    24421 tgctaaaaat ggtattgtaa tgtggtcctc tccttcaaat ttcttgttgt attcaatcgt
    24481 tgtttctatt gaatgctcgt ttcttgccca gcttcttctt gacaccccga ccattgtatc
    24541 ccaaggaatg gaagttttta ttatttcatc gactcttttg cttccgtcaa gaacaagacc
    24601 gaaccccccg ttaatcgcct tgctgacccc tactcctccg ccgttgtgaa gtgcaaccaa
    24661 ggacataccc cttgctgcat ttcccgcaaa gcagtgaacc gccatatctg ccgtaacatt
    24721 gctgccgtca tatatgtttg atgtttccct gaaaggagaa tccgttccgc ctgtatcatg
    24781 atggtctctc cccagcatta cagggccgat ttcacctttt cttaccatat cattaaattt
    24841 aagtgctatg tcacgtcttc ccaatgcatc ttgataaaga attcttgctt gggttcctac
    24901 aactaattta tttttctctg catctctaat ccaaatataa ttatctctgt cctgacctct
    24961 tctgttaggg tctatcacag acattgcagc ttggtctgtt ttatgtaaat cttcaggctt
    25021 tccgcttaag caaacccacc tgaagggtcc gtatccgtaa tcaaaaagca tcgggcccat
    25081 tatatcttcc acataggaag gaaatataaa tccttcgctt gtatcattgc cgttcttggc
    25141 aatttccttc actccagcat caaatacagc cttcatgaat gcattgccgt aatcgaagaa
    25201 atatgagcct ctgtcaacca aagtttttat tgctttaaag tggtcagcca atgacttatt
    25261 taccatctgt acaaattttt ctctgttgct gtttaataat tcagttcttt cttcaaaggt
    25321 aagtcctttg ggacaataac ctccgtcata aggtacatgg catgaagtct ggtcggataa
    25381 caaatccaca tgaatgttgt tatctgctgc atattccaac aagtctacaa tgttaccgtg
    25441 gtatgctatt gaaattgttt gttttttgta caggtactcc cgtgcaattt caaatgcttc
    25501 ctcaagatta tcggtaaccg tggaaaccca tccttgtttt aacctggttt caattcttga
    25561 gtagtcaacc tctgcaatta tacctacgcc gttggctatt tcaacagctt tagcctgtgc
    25621 gccgctcatc cctccaagtc ccgaggttat gaataaatgt cctgccaaat ctttatcatc
    25681 agtgataccg agttcttttc tgcctgcatt tataagtgta ttaaacgttc cgtgaacaat
    25741 accctgaggt cctatgtaca tccagccgcc tgccgtcatt tgcccatagc ttgaacatcc
    25801 cattgcggtt gccttcgccc aatcctgcgg attgtcaaac attcctacca taagtccgtt
    25861 tgatattata actctggggc tgtttttatg ggatttaaac aatcccaacg gatgaccgga
    25921 cataagggct aatgtttgct cttccgttaa tgcttcaaga tattttttta ttaattgata
    25981 ctgcatccaa ttttggcaaa cctgacctgt ttccccgtag gtaaccaatt catacgggta
    26041 aagggcaact tcatggtcta aattattgtc aatcataact tggaatgctt tgccttctat
    26101 acatttcccc ttgtactcat ctataggctt accgtaaatt ctttctttag gcatgaaacg
    26161 gtagccgtaa atccttccat atgtcatcaa ttcatccaaa aattcgggag ctaatttttc
    26221 atggagactt cccggcacat accttaatgc attcttcaat gccagcttta tttctctttc
    26281 atttaaagta acttctcttt tgggagcacg tcttacattt tcctttaaaa ccggatactc
    26341 tggtaattca tcgcttaact tgattttcat tgcattaaaa atatttaaat tatcagtcat
    26401 attacacctc ctaaaacaaa ttaaaaaaaa ttttatatct taagattact taaagctttt
    26461 tcgggtaaac tatatataag ttaaatagta aatttaactt ccctctcccc cttgtttttt
    26521 tataaacggt tattatgcct tacttatgta aggcataatt ttttttagtc acagcgcgat
    26581 atttaatcgc catcttgtca ggataatatg aaagttttgc tttccttaag ccttcagagc
    26641 ccatgtcttc ttctctgttt atgtaaatca aatgaggata tttatcttta atccaggaag
    26701 caaatcgttg gtttatcatc ggatatgccc cttgaacatc tttaaacgct ttttcgatat
    26761 gtaatacata tgtatcatta cttagaactt cacctatggt ataagccaca attttatctt
    26821 ctaccctgat taaaccgcct tcaagctcga gctcgtcata atatttaaag aaatatttag
    26881 tggcacagtt ttcatccata agactttcat cgtctttaca tcctgattca atgcaccatt
    26941 ttttgttcat ttcgatgcat tcagctttat tttcctcagt tattgtttca aatgaccaaa
    27001 caggattatt tcttagaaag gcactgatat ggtttctttt tctgtgaagc tttttccctt
    27061 ttaatgaaac cattttttcc aacagataaa tatagtcaaa gtcatttctt acttgatcat
    27121 aaataaattt ccccggatac aattcattca gttcaatcat ttcatcatga gataatcctg
    27181 caaagattag atttttatct gcagacctta ttatttcttc aattacgggt ttaacatctc
    27241 cttttcccat tggataaaga tatacatctt ttccattaac taattgtttg gctaccaaaa
    27301 aattattgat attagcaact ctagtgttat ttatgtccct ccatgcaaac aaatttccga
    27361 aattctgatg acatcctgta ataaccgatt ccttcaatat gggaataatc cattctttat
    27421 cttccagttc aatcttttta aagttcagca ttaataattt tctccttgtc acgttgtcca
    27481 atcgtatagt attttttcct cattagaaat atattacatt acacattata cactatatca
    27541 taaaaaaaat caccaattac tgttttcgtt gttcgtattt ttcgaacaaa tacttcacta
    27601 atagatttaa cagcacaata aaaataatat agaatattga ctttgcagga ttttcaaaaa
    27661 atgagcttac attttcaccg ataaaagcca atgaaagaag catcacaaat tttgacggca
    27721 gtaatatagc aaggaacatt tccgtcctca tattagtcaa agctgcagtg ccgctcagta
    27781 aaaatgacgg agcaaaagga aagcagtata ataaaaacag cacgtttaat tgttttttct
    27841 ttaccttttc aagggtgtag cgaaatttgc tactttttat tttttcattt attctttttc
    27901 ctccgatttt ccttaacaat aagaataaaa gaaacgatcc taagcaatta ccaatccaag
    27961 aatataagta tccgaagaaa aatccaaaag cagctacatt tattattaca aatcctacca
    28021 atggcagtat gggtaaaaat gcttcaaaca caggaagaat tatggctgca gcaggtcccc
    28081 acgtttctaa atattttatg aaaatttcag aatattccgc aaattccaaa ttcattatca
    28141 aacctccacc ggtcagacta tctatattta ttatacccat cattagattt acatacaaaa
    28201 caaacaaaac atttatataa attaagtgaa acactattaa tattaacata aatgcttgat
    28261 aattttaaat aaaaaatata aaattatctc atgagattct tcacagtgtt cagaatgaca
    28321 atatgtgtat tcatggcaag acagggtaaa taattataaa aaagatatag aaaggattta
    28381 tatgaaatat ttcataggag cccccatacc tgataactat aaaaataaaa ttgaaatgct
    28441 aagagctgaa tttagatttt tcacaaccga acctcacatt actttggttc ctcctcctgc
    28501 acttcctgat gaggatgact ttattgagga agtagttgaa gtatgcaaaa aagttaagcc
    28561 ttttgatata aggttgaata atcttgacca gtttggcagc agagttttat atgtaagtgt
    28621 tgattctccc ggtttaatcg atttacataa taaaatatat gaaaaattat gcttgcagca
    28681 tgagagaagg ggatttaccc cacatttaac aattgtaaag caaagaccca aaagacctgt
    28741 agatatagcc acaataagaa aaagggcaga aataaaactt attccttccc cagaatatac
    28801 tttgaattca attaccgtct accatcaacc aaaggagcat tccatttata ttccctacat
    28861 ggaaattcct ttgggagaat aac
//
LOCUS       DAOPUE010000012        28259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1635_length_28259_cov_10.475323, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000012 DAOPUE010000000
VERSION     DAOPUE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1635_length_28259_cov_10.475323"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKH42_02190"
     CDS             complement(<1..464)
                     /locus_tag="PKH42_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019849223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HOA19268.1"
                     /translation="MEYRNANINDIYGVSQLQKKYHIYTISEEDKPDGFVTTLFTEEQ
                     FKELIEKENGLAIACDNDKVIAYAMAASWDYWSAWPLFQYMINDLPNIQYLGYTLSTE
                     NSYQYGPICIDKQYRGTEVLPNLFEFSRLQMKDKYPVLITFINQINGRSYRA"
     gene            701..1444
                     /locus_tag="PKH42_02195"
     CDS             701..1444
                     /locus_tag="PKH42_02195"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOA19269.1"
                     /translation="MKKLDGRVAIVTGSTSGMGRATAVLFAREGAKVVVTGRNEERAK
                     AVVDQIKGEGNEAIYVLVDTSKVEDVKKLVDVTLDTYGTVDILFNNAGALSMSPITDV
                     SLEEWNKIFNVNVTSALYLTQLVAPIMKAKGKGVIINTSSVAGFGAHHGFAAYVSSKH
                     AMNGLTKSMAWELGPEIRVNGIAPGLIHTAMVDSIGGPSVVQHMIDQSPVKRVGLPED
                     IANAALFLASDDSSFIDGQIIKVDGGFEI"
     gene            complement(1521..1793)
                     /locus_tag="PKH42_02200"
     CDS             complement(1521..1793)
                     /locus_tag="PKH42_02200"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOA19270.1"
                     /translation="MVNIKYIMFYLLIINLISFALFFADKQKSKRDKWRIKESTLHLV
                     SFMGGTLGSIAAMILFHHKTKKPKFVIITIIALIFNVVLVYELLIR"
     gene            complement(1786..2430)
                     /gene="trmB"
                     /locus_tag="PKH42_02205"
     CDS             complement(1786..2430)
                     /gene="trmB"
                     /locus_tag="PKH42_02205"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOA19271.1"
                     /translation="MRLRYVPGQYQYLKNCEKVITEPEKQGFKLNKVFNNNNPIHVEL
                     GCGKGKFLRENAINNPHINYFGFEKSVKVAYRCAKSAFENDPPNYYIVYSNGDILKEV
                     FDAGSIDRIYLNFSDPWPKPSHYKRRLTHKNFLDVYKIVLSKNGEIHMKTDNNDLFEY
                     SLEQFEKDNWEFKMVTRDLHNSIHKEDNIMTEYEERFVNKGIKINKLIVRRKNG"
     gene            2771..3304
                     /locus_tag="PKH42_02210"
     CDS             2771..3304
                     /locus_tag="PKH42_02210"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOA19272.1"
                     /translation="MDFFMNLEGPLLYFIIFFAKIIEVSISTVRIVFINKGEKIKGAL
                     LGFIEIMIWLIVVSSVLNNITEDPIKVFIYAIAFSLGNFFGVTIESKIAVGLSSIQVV
                     VDQKEGEVLADILREQGFGVTILEGKGKNDSVKNLLFIQLKRKKIPVATKLIKEHNPE
                     AYITVNEIKTMFGGYIK"
     gene            complement(3387..4709)
                     /locus_tag="PKH42_02215"
     CDS             complement(3387..4709)
                     /locus_tag="PKH42_02215"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOA19273.1"
                     /translation="MLKVAKFGGTSLADSKQFNKVYDIIKNNDERKYIIVSAPGKRFK
                     DDNKITDLLYLTYAHIKYSVPYAPILKIIEDRFRQIKEELNLKTDLDYEFKIIKENLD
                     NKCEEDYIVSRGEYLCALLMADYLDCDFIDAKDVIFFNYDGTVNEEKTHEKLTEILGK
                     SNKAIIPGFYGSYPDGSIKTFSRGGSDITGSIVSSVMGADIYENWTDVSGFLVADPRI
                     VNNPKQIKKITYQELRELSYMGASVLHEEAVFPVRQAGIPINIKNTNDPENPGTLIIR
                     DDQAGEDYANGHIITGIAGRKDFSFFYIHKEHMAHEVGIVKKALEVFEERGISIDHIP
                     SGIDSFTIVLPSESVEKISHQIVEELKKKCKTDNVQVFRNLSLITTVGIKMAYRPGIS
                     AKLFTALGNRNINIRMINQGSSEINIIVAVEDKDFEEAIRAIYDAFIK"
     gene            complement(4805..5788)
                     /locus_tag="PKH42_02220"
     CDS             complement(4805..5788)
                     /locus_tag="PKH42_02220"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOA19274.1"
                     /translation="MNKIRIGIVGYGNIARGVEAAVNLSTDMELVAVFTRRQPDTIKT
                     QTDVKVLSVDEVEKYKDIIDVMVLCLGSATDLPVQAAEYAKMFNTVDSFDNHKKIPEY
                     LRKVDEGALSSGKISIISIGWDPGLFSIMRMLSESVLPEGSSYTFWGRGVSQGHSDAI
                     RRIPFVKQAIQYTIPVEDAVNCVRRGDNPELTAREKHIRQCYVVCDECADKNKIEEDI
                     KNMPDYFAEYNTEVNFISEEEFKKNHSRMSHGGLVIRNGMTGSEKNQHIMEFSLKLDS
                     NPEFTGSVILSYARAAYRMNKEGIKGAKTIFDVPLSYIHPEGREKLIRNFL"
     gene            complement(5815..6483)
                     /locus_tag="PKH42_02225"
     CDS             complement(5815..6483)
                     /locus_tag="PKH42_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOA19275.1"
                     /translation="MNVVICGINGAMGRVLHEEIKTYDNINLIGSLSPRSGRFGQDID
                     GMPDVVIDFSNTANIDFLIEYATKNKCALLICTTGFSDEDKAKIKEAGKAIPVLLSSN
                     TSLGINVFRKILKSISAELSTFDINIVEKHHTKKIDAPSGTAKTLSEDIKNATGREHV
                     EISAIRAGTIPGEHTVIFSGVDEILEIKHTAFSKKIFARGAINLAMKLSSKSPGYYLM
                     EDFF"
     gene            complement(6492..7388)
                     /gene="dapA"
                     /locus_tag="PKH42_02230"
     CDS             complement(6492..7388)
                     /gene="dapA"
                     /locus_tag="PKH42_02230"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOA19276.1"
                     /translation="MNDLVRGCGVALVTPFADGEIDYEVLGKLVEMHVENSGSIIVAG
                     TTSEAATMTDEEYHTVVEFVIDRVNKRIPVIAGAGSNCTQAAVKKAQFCEEAGADGLL
                     IVTPYYNKTTQRGLVNHYKIIAESTSLPIIVYNVPARTGMNIEPKTVLELSKIKNIVG
                     IKEASGDISQVAEIIRICPDDFLVYSGNDDMVVPLMSLGGHGVISVSANILPAEMKKM
                     VDDYRNGNVNDAKNAQLALNGINKALFIETNPIPVKEAMSIMNLCSSEVRAPLYQMSE
                     ENRARLLRELNLFGIGVPLETA"
     regulatory      complement(7561..7711)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            7953..10274
                     /locus_tag="PKH42_02235"
     CDS             7953..10274
                     /locus_tag="PKH42_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOA19277.1"
                     /translation="MKNMLCSICKKNVAVIYVNKIVEGKMQTTGLCIPCAKKQGIAPM
                     DQIMRQSGMKPEDFDNINKQLTEIIGDMDLEKLSGDMSESSDGINSLMNLMNNAFTGN
                     TSSTSNEFIPEDEDADYTGPGSGTGPKIKEKVKRKKLKYLDTYGLNLTEQARFGELDR
                     IIGREREIDRVVQILNRRSKNNPVLIGEPGVGKTAIAEGLAVRIAEKKVPVKLFNTEI
                     YLLDLTAVVAGTQFRGQFEARMKAIIDEAKKAGNIVIVIDELHNIVGAGEAEGGAMNA
                     ANILKPALAKGDVQIIGATTLDEYRKHIEKDTALERRFQPVIVDEPSIEDSIEIIKGI
                     KDYYEQFHNVKITDEIIRQAVLMSERYITDRYLPDKAIDVIDEAGSRANLKNKGLVEL
                     EALKAELSRIRDEKEEAAENDDYERAAELKVQEIRIESKLKELSNEYENILLTEEDIA
                     YVIEAWTKIPVQRISEAEADKLIHLEERLHRRVVGQHPAVTSLAKAIRRNRSGFKKKK
                     KPSSFIFVGPTGVGKTELARTLAYELFENEDAMIRLDMSEYMEKHTVSKLIGAPPGYV
                     GFDQAGFLTEKVRRKPYSVILLDEIEKAHEDVFNILLQILEDGRLTDNQGRTVNFENT
                     VIIMTSNAGTSIKANRIGFGNTEYEAMESKVKDALKQAFRPEFLNRIDDIIVFSELTK
                     EELSQIVDLMLKDVIKEAEEKKITLTVTDKMKEHILEKGYNTKYGARPLRRAIQKYVE
                     DEISEAYLKGVFKENSLVTVDVNDEGKTVLTII"
     gene            complement(10417..11259)
                     /locus_tag="PKH42_02240"
     CDS             complement(10417..11259)
                     /locus_tag="PKH42_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOA19278.1"
                     /translation="MGIRIVIDSTSDVTDEIIKKYNIKMVPLTVNFENESFLDKVELN
                     SKQFFEKLETAEKLPTTTLVSPGTFMEVFSDILLEGDKVLGLFLASEFSGTYDSACMA
                     KNMIGSDDIYIIDSRSVCLGAFALILEAIELVQQNKDIEEIVKELEALREKVVAVAAL
                     DTLKYLEKGGRLSKGQAVVGTLLNVKPIIEIKDGKINVIEKIRGKNKAVKWFDEWIAK
                     NNYDLSNKTVLLFHGKAYEQLKMLRESIEDKYKIKNIIEQEIGAVIGTHSGPGVLGIG
                     FVNK"
     gene            complement(11298..11753)
                     /locus_tag="PKH42_02245"
     CDS             complement(11298..11753)
                     /locus_tag="PKH42_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOA19279.1"
                     /translation="MSNHETVNEILVRLFANILYIEEKCLKIGEFKDLTITEMHLIEN
                     IGYSTERTMSDTAKAVKVTSGTLTTAIDNLIKKGYVERRRSSEDRRVVKIRLTEKGKA
                     AYKSHEDFHKDLVISALQQLNSEEEEVLIRVLTNIDKFFSEKYKYAGWY"
     gene            complement(11808..12404)
                     /locus_tag="PKH42_02250"
     CDS             complement(11808..12404)
                     /locus_tag="PKH42_02250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOA19280.1"
                     /translation="MKLNRNFYNRDARIVGEDLIGKILVRKYNNTVIKARIVETEAYI
                     AAIDKASHGYGGKITPRTKIMFGPPGYAYVYFIYGMYYCLNFVTEEEGTCSAVLIRAV
                     EPVINKEEMCFNRYKEHLDDITKYQKKNISNGPGKLCNALKITIEHNGTDLTGNEIYV
                     EDDGFKDFKVEKSKRINIDYAEEAKDFLWRYTIHHKKA"
     gene            complement(12454..13251)
                     /locus_tag="PKH42_02255"
     CDS             complement(12454..13251)
                     /locus_tag="PKH42_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOA19281.1"
                     /translation="MVSKFIDSHAHLDDKIFETDRDNLISSLYDNDVEAVLNPGADLQ
                     SSKEALLMANKYSFIYAAVGCHPHDTKYMSDEHMNEFIEMAKNKKVIGIGEIGLDYYY
                     DNSDRDTQRKWFREQIRLAKELNLPYIVHDRDAHEDILRIMKEEHYDGARGILHCYSG
                     SVELSKEFIKLGFYISLAGPVTYKNARVPKLVAKEVPFDKLLIETDSPYLTPVPYRGK
                     RNEPKYVKYVAEEIARIRNVSIDEVAEKTKENFKRLFNIEEGGHTFK"
     gene            complement(13241..13792)
                     /locus_tag="PKH42_02260"
     CDS             complement(13241..13792)
                     /locus_tag="PKH42_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19282.1"
                     /translation="MAAVSSIFYPLIFLVLIYYLFKNTKNRMQYKKENMEALLFLDED
                     DKRVHLMSRLLMALMILISGIMIRGLIETKTYAAEQVLIMGVLPILIITLYIPLSKKT
                     MISTLGIHKRGNLIRWEDIKGINYPKPDEKGKVKVNIIYSVMNKDSNIHLTFLKNDPQ
                     LDKFKEHAKTYRNTKKKDKKSGK"
     gene            complement(14029..14847)
                     /locus_tag="PKH42_02265"
     CDS             complement(14029..14847)
                     /locus_tag="PKH42_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19283.1"
                     /translation="MWEKKFEPVANISEEHKEYLKLINREKNFIRIAQILIFVFGIIF
                     WEVAARKNLIDTFLTSSPGAILGLIMKLVDDGSLFKHLFISTMETIGGFFAGTVGGLL
                     IAIILWWFRKLAKVMDPYIVILNSLPKTALAPIIILWVGMGYSGIIATAASISIVVTT
                     MTLYTGFSNVEKDKIILLKTMGANKFQILKKVIIPANIPNILSVIKVNIGLSWIGVIV
                     GEFLVSKAGIGYLILYGSQTFKLDLVMMGVFVLGVIAAIMYVFSSWLEKKIIKY"
     gene            complement(14828..15601)
                     /locus_tag="PKH42_02270"
     CDS             complement(14828..15601)
                     /locus_tag="PKH42_02270"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19284.1"
                     /translation="MTEVDMVYICELRNVSKRFHTINNETKAIDDISFKVKKGEIVSI
                     VGPSGSGKSTVLSLIAGLEKPSEGEVIVNTDSIGFMFQKDQLYEWRNVYKNCLLGLEV
                     KKMDNEENKIKVKRMLEKYGLTNFKNHYPSQLSGGMRQRAALVRTLAIEPELLLLDEP
                     FSALDYQTRLSIGDEVGTILRNEKISTILVTHDIPEAVSISDRVIVFTERPAKIKKEY
                     EIKFEECPGERTPMKCRNTLAFGEYFRNIWRDLDVGKEV"
     gene            15740..15868
                     /locus_tag="PKH42_02275"
     CDS             15740..15868
                     /locus_tag="PKH42_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HOA19285.1"
                     /translation="MFKKLTNKLKMRYRRSANPGNVNIPGEFEVQERKKDVDNNKK"
     gene            complement(15977..16408)
                     /locus_tag="PKH42_02280"
     CDS             complement(15977..16408)
                     /locus_tag="PKH42_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOA19286.1"
                     /translation="MKEEFYTLKGYKIKNEEILSSSMEDYIEMICRLSEKSDEVRVSE
                     LSSALNVQPPSTTKMIRRLAKENYVTYERYGFIKLTEKGREVGSKLLERHRIIFEFLE
                     CIGVKENILEQTEIIEHAVNDEVLNKIDELTQFLKKCDKYK"
     gene            16533..16751
                     /locus_tag="PKH42_02285"
     CDS             16533..16751
                     /locus_tag="PKH42_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOA19287.1"
                     /translation="MKLADVGVGKTVKVKQNSAHDYLKERFLALGLTKGADVEVLRKG
                     PKNNLTVYRIRGAMIALRNEESSLIEVL"
     gene            16763..18682
                     /locus_tag="PKH42_02290"
     CDS             16763..18682
                     /locus_tag="PKH42_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOA19288.1"
                     /translation="MGLTAQSVKLESLRDIYEIEKNDKQYVVALAGNPNTGKSTVFNY
                     LTGLNQHTGNWPGKTVVSARGQFKYKNNEYILIDLPGSYSLFTASKDEEVTRDFLCYG
                     NYDTVVVVADATCLERNLNLVFQVSELTNKIILCINLIDEAKKKNIKIDKEGLEKELK
                     IPIVLTSARNGQGMKELKDLIEKTSQIIEEETEKSLYFNQDESIREKISEDNYNYANK
                     LKQKYVKEDVNKLNRDKKIDDIVTSKKFGIPIMLALLGLIFWITIQGANIPSQILADL
                     LFGLQYKLDKILLILGVNDFIRGVFIDGMYKSLAWVISVMLPPMAIFFPLFTLFEDLG
                     YLPRVAFNLDHLFKRAGAHGKMCLSMCMGFGCNAAGVIGCRIIDSPRERLIAVITNNL
                     VPCNGRFPLIIVLSAAFFAFTGSFVDSIIPALSITLIVLIGVFATLSVSYLLSNTLLK
                     GIPSTFTLELPPYRAPQVGRIIYTSLIDRTIFVLSRAAVVAAPAGAIIWLLANIYVGE
                     LSILIHMANFFEPLGRAIGLDGFILIAFIAGFPANEIVIPVLLMAYMSTGTLTDFESI
                     DSLRQILISNGWTQLTAINVMLFSLLHWPCSTTLLTIKKETGSLKWTLAAFIIPTGIA
                     FLVCFITANLYRIIW"
     gene            complement(18808..20454)
                     /gene="gltX"
                     /locus_tag="PKH42_02295"
     CDS             complement(18808..20454)
                     /gene="gltX"
                     /locus_tag="PKH42_02295"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOA19289.1"
                     /translation="MDYKKLAELLFPNITKTIEYYENEVYPKRKLPEGAKVTRLAPSP
                     TGFIHLGNLYGAFVDERLAHQSGGVFLLRIEDTDEKRKVEGSVETIITSLEYFDLKFD
                     EGAGIEGETGDYGPYFQSNRAEIYQCCAKHLVEIGRAYPCFCTEGELEELRKEQAKEN
                     VNTGYYGKWARDRNLSLEEVQQHLNNNEEFVIRFRSEGTEEGKFEIVDAIRGRLIMPE
                     NYQDIVILKTNGIPTYHFAHVVDDHLMGITHVVRGEEWLSTLPIHYEIFKTIGWETPV
                     YCHTAHLMKMDNGAKRKLSKRKDPELGLGYYRELGYHPKAVREYLLTILNSNFEEWRM
                     KNPENPIDDFQFTLGKMSNSGALFDLDKLNNICKDVLLKIPAAEIYEFLLNWANEYKK
                     EAYELLKSNKDEVIKLLSVGRDGNNPRKDLMYCEQILDFISYYFDEYFKIVDSYPENI
                     DSEEAKKLLKAYLSTYDHGDDQSQWFEKIRVIASENGYAAKPKDYKKNPDMYKGHVGD
                     VSTAIRIAIIGRASSPDLWELQQIMGEEKVRRRIENAINS"
     gene            complement(20545..20616)
                     /locus_tag="PKH42_02300"
     tRNA            complement(20545..20616)
                     /locus_tag="PKH42_02300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20581..20583),aa:Glu,seq:ctc)
     gene            complement(20805..>21182)
                     /locus_tag="PKH42_02305"
     CDS             complement(20805..>21182)
                     /locus_tag="PKH42_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19290.1"
                     /translation="KEVFAEENREENITDFYDEFAFNAVLYEDDVPAVTARLSFKEGK
                     YFIDKLCIKKQFTGLRYEDLLVRLLVRKAVTIGAEKTYIEADESIEKILENIGFTKVP
                     NNKTLMVKEGDVGGHCCGDCPEK"
     assembly_gap    21183..21282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21569..21823
                     /locus_tag="PKH42_02310"
     CDS             21569..21823
                     /locus_tag="PKH42_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOA19291.1"
                     /translation="MDKRIGVIGIVIEDTSQIAEVNRLLHEYSHIIIGRMGIPYREKG
                     LNVISIIVDGTNDDIGALSGKLGKIKGLSVKSALSKNIRR"
     gene            21825..22853
                     /locus_tag="PKH42_02315"
     CDS             21825..22853
                     /locus_tag="PKH42_02315"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA19292.1"
                     /translation="MKKISFLLVVLIVLIFTAGCNNEPAETPTPDENAEETPMPEEPA
                     DVKPVNVTIAGLKGPTSIGMIKMIDEKALKSDKYNVEYIQESAPDSLTGKIINGDIQI
                     SSVPINLASVLYNKTQGKVQLLAVNTIGNLYIVGTEDISSVSQLECKTLGMSAKGSTP
                     DFAMNYILKQAGLEGKTELNYALDHATLAQSVIAEDTRVALLPQPFVTQTTLKNPNVK
                     MLIDLNEAWNDATGGKSELFTGCIIINKEFAENNPEFVDEFLKQYENSVNWVLENQKE
                     ASVLTAKHEIMPDAVLVEKALPYCGITFRKAAEAKESLNNFYQILFDSNPASVGGSMP
                     DDEFYFKE"
     gene            22828..23577
                     /locus_tag="PKH42_02320"
     CDS             22828..23577
                     /locus_tag="PKH42_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOA19293.1"
                     /translation="MMNSILRNKNKLLIILFWIFVWEILSLIIAQEIYLPSPHAAFSS
                     LMNLLISKDTYITIYYSTYRTLTGFFLSCVFGIFLGIISGIHKTVYNLLNPLIVIIRT
                     IPVMSIIIIAIMWFKDTNVPIFVAFLMCFPIIWTNTVSGILSTDVKLLQMCEVYKIKK
                     SRIISSVYFYSSLPYIKAGMISALGIGWKVTSAAEVLSLPKYSIGRFLYDSKVYLEIP
                     DLFAWTIIIVSLSFLFESLLKTIFKFGDKYD"
     gene            23570..24247
                     /locus_tag="PKH42_02325"
     CDS             23570..24247
                     /locus_tag="PKH42_02325"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19294.1"
                     /translation="MIKLINISKKYDNLILLKDFNIDFEKNKITCLFGPSGVGKTTIA
                     NIVAGIVPVDEGRVVGIDNSLFSYVFQEPRLLKWYNVYENIDFVLKDVYKPDKRAEII
                     NSYLEMVELSEYRNYKIDALSGGMAQRVSLARAFAYPSHILILDEPFKGLDNKLKGEM
                     SSLFLKMWNLSQRTVIFITHDVEEAVDLSHIIYTLKDRPVNIIDRLIVKNYANNKEEI
                     KNLLLNH"
     gene            complement(24733..24999)
                     /locus_tag="PKH42_02330"
     CDS             complement(24733..24999)
                     /locus_tag="PKH42_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19295.1"
                     /translation="MECNKPENIVEAKADVNTQGDCVRGDRGRRDCIRRDRDRVMGTG
                     GIDSSLLFFFLLLVIIVDQSDCDIEFDTLLWFFLVLVAIFCKCD"
     gene            25159..25356
                     /locus_tag="PKH42_02335"
     CDS             25159..25356
                     /locus_tag="PKH42_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOA19296.1"
                     /translation="MDKFALFLVIIGAINWGLIALFQFDLVAAIFGGQTALFSRLVYG
                     LVGLAGLYCITLLFNERERVD"
     assembly_gap    25358..25457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25458..26803)
                     /locus_tag="PKH42_02340"
     CDS             complement(<25458..26803)
                     /locus_tag="PKH42_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOA19297.1"
                     /translation="MEMKSLIDKIFQKGREKGLNDMEVYYSAGSSLTLKVFQKELETY
                     NLSESEGLSLRGVYKGKMGYSYTEKVDETSIDQLVRDVLENAVIIDSDDEEFIFEGSK
                     EYIKVDSFNPALSNVSEEKKIEFVKSLEEEAFKLDKRIESVEVCVYGDGYGEIIMSNT
                     KGLFLQDKSNIAYTYIVVVAKEGEDIKTGMAYRTGNDFNKFNARDIAKEAVDEALALL
                     GAKSVKSGDYNVIIRNSAAADLLEAFTGIFSAEAVQKNLSLLKGKLNEKIASDKFTLI
                     DDPYMESGLASKSFDGEGVACKYKKVVDKGVLKTYFHNLKTAKKDGVETTGNASKGSY
                     KSSLGISPSNFYVEKGERRLNEMITDINKGILITELQGLHSGLNTVSGDFSLAALGFL
                     IENGKISRPVEQITVAGNYFEMLKNIEETGSDLKFGLPGGSYIGSPSLKIKKLSIAG"
     gene            complement(26806..28194)
                     /locus_tag="PKH42_02345"
     CDS             complement(26806..28194)
                     /locus_tag="PKH42_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOA19298.1"
                     /translation="MLSRKLIENTLTAALSTGGDFAEIFVEDRTNNGIVMIGGKVEST
                     MSGRDYGVGIRIFKGFNSVYAYTNKSDEDTLIETAKKAAQAVEGNIIDLTINLTKSDV
                     KNINIIQIDPSKVEKTKKVQVMKKAYDIAFNYDELITQVSINYTDYIQNIMVANSEGL
                     WKEDTRVRSRLGISSVASKDGEMQSGFFAPGASKGYEFFENLDIDYYAKEASRIAVTM
                     VKAKYSPSGRMPVIIDNGFGGVIFHEACGHGLEATSVAKGLSVFAGKIGQQIASPIVT
                     AIDDGSIPNEWGSFSIDDEGTQSQRNVLIENGILKGYMIDKLNARRMNMDITGSSRRQ
                     SYRFPPTSRMSNTFIDNGSSTPEEIIANTEKGLYAKHMGGGSVNPATGEFNFAVMEGY
                     IIENGKIKEPVRGATLIGKGMEVLNKIDMVGNNLLQSQGMCGSVSGSIPANVGQPMIR
                     VSELTVGGREGE"
BASE COUNT         8546 a         5152 c         4582 g         9779 t
ORIGIN      
        1 tgggcacggt atgatcttcc gttgatttgg tttatgaatg ttattaatac agggtatttg
       61 tctttcattt ggagtcttga gaattcgaaa aggtttggta atacttcggt gcctcgatat
      121 tgcttatcaa tgcatatggg accatattgg taagagtttt ctgttgaaag tgtgtatcct
      181 aaatattgga tgttaggcaa gtcatttatc atgtattgaa aaagcggcca agctgaccaa
      241 taatcccatg atgctgccat tgcatatgct ataactttgt cattatcaca tgctatggca
      301 agcccgtttt ctttctctat caattctttg aactgttctt cagtaaacaa ggttgttaca
      361 aaaccgtcag gtttatcttc ttcacttatt gtatatatat gatatttttt ttgaagctgt
      421 gaaactccgt aaatatcatt tatattagca tttctatact ccatagttgt tctccttttc
      481 atttgtataa atttttgttg agtttttatt taatctaata tcattaaaca ctatgaaatc
      541 gatatcataa tactattata ccatctattt ccaaatattg aaaccctgtt aataattatt
      601 ttgaggaaaa ggttatcgtt atttttttaa cattgactta atacttatct tattataaga
      661 ttaactataa caatgattac taataaaagg aggggttcaa ttgaagaaat tagacggcag
      721 agttgcaatt gttaccggtt caacatccgg aatggggcgt gccactgcag tactatttgc
      781 tcgtgaaggc gcaaaggttg ttgttacagg aagaaatgaa gaaagagcaa aggctgtggt
      841 agaccaaatt aaaggtgaag gcaatgaagc gatatatgtt ctagtcgaca catcaaaagt
      901 cgaagatgtg aaaaaattgg ttgatgttac tcttgataca tatggaactg ttgatatatt
      961 attcaacaat gcaggtgctc taagtatgtc acctataaca gacgtaagtc ttgaagaatg
     1021 gaataagata tttaatgtaa atgttacatc agcattatat ttaactcaat tggttgcgcc
     1081 tataatgaaa gcaaaaggaa agggcgttat aattaacaca tcttcggttg caggatttgg
     1141 tgctcatcat ggttttgctg cttatgtttc atcaaagcat gctatgaatg gactgacaaa
     1201 atcaatggca tgggaattgg gaccggaaat tcgtgttaat ggaattgcac cgggactgat
     1261 acatactgca atggttgaca gtatcggagg tccttctgtt gttcagcata tgatagacca
     1321 gagtcctgtc aaaagagtgg gacttcctga agatatagct aatgctgcac ttttcttggc
     1381 ttctgatgat tcatcattta ttgacggaca gattattaaa gttgacggtg gttttgaaat
     1441 ataagtacca taataagata aaatgactga gctgagtgct cagtcattat ttttatcata
     1501 taataattga aatactgctt ttatcttatt aagagttcat ataccaatac cacattaaaa
     1561 atcaatgcaa taattgtaat tataacaaat ttaggcttct tggttttgtg atgaaacaaa
     1621 atcattgctg caatgcttcc aagtgttcca cccataaaac ttaccaaatg aagagtgctt
     1681 tctttaattc tccatttgtc tctttttgat ttttgtttgt cagcaaagaa taaggcaaag
     1741 gagattagat ttattattaa taagtagaac ataatatatt ttatgttaac catttttcct
     1801 tctcactatc aatttattta tttttatacc tttatttaca aatcgttctt catactcggt
     1861 cataatattg tcttccttat ggatgctgtt gtgcaaatct cttgttacca ttttgaattc
     1921 ccagttgtcc ttttcaaatt gttcaaggga gtattcaaat aggtcattgt tgtctgtttt
     1981 catatgaatt tcaccatttt tggaaagaac aattttatag acatccaaaa agtttttgtg
     2041 agtcaatcgt cttttgtaat gagaaggttt aggccaaggg tctgaaaagt tcagatatat
     2101 tctgtcgatt gaacctgcat caaatacttc ctttaatata tctccgtttg aataaacgat
     2161 gtaataattc ggcgggtcat tttcaaaggc tgatttagca catctgtatg caaccttgac
     2221 acttttctca aatccgaaat agtttatatg ggggttatta attgcatttt ctcttagaaa
     2281 ttttcccttt ccgcaaccta attccacatg tatcggattg ttgttgttaa atactttatt
     2341 cagtttaaat ccttgttttt cgggctcagt aattactttt tcacaatttt ttaaatattg
     2401 atactgtccc ggaacatatc ttaatctcat gtttattttc ctttttatat aagtaaaatt
     2461 tattatacat tattttaatg aaaattaaca gacgatattt tactattggt ttaaaagatg
     2521 agattcttca cttgcgttca gaatgacaag agtagagatt cttcgagcgg aagctctcag
     2581 aatgacaaga gtataggttc ttcacatccg ttcagaatga caaaaagatt tacataaata
     2641 aaattatgta accctattgc tgcgtattat tataacaaaa ataatcagct gtctttaata
     2701 aaataatatt tgcgtatatt gcatattact gataaaatga gtagaataaa tttggaggtg
     2761 gacataacaa atggattttt ttatgaattt agagggtccg ttgctgtact ttataatctt
     2821 ttttgcaaaa attattgaag tttcaatttc aacagtgaga atagtcttta ttaataaagg
     2881 agaaaaaatc aagggtgccc ttttaggctt tatagaaata atgatttggc ttattgttgt
     2941 aagctctgtt ttaaacaata ttactgaaga tcctataaag gtttttattt acgcgatagc
     3001 attttctttg ggcaatttct ttggagttac tatagaatca aaaattgccg tgggattatc
     3061 ttcaatacag gtcgttgtag accaaaaaga gggagaggtt cttgcggata ttttgaggga
     3121 acaaggattt ggtgttacga tactcgaagg aaaaggtaaa aatgatagtg ttaaaaatct
     3181 tttatttatt cagttgaaaa gaaagaaaat tcctgttgct acaaaattaa taaaagagca
     3241 taatcctgaa gcatacatta cagtaaatga aataaaaact atgtttggcg gatatataaa
     3301 ataattattt gataaaatta ttaaaacaca gctaatggta aaggggacac acccttatag
     3361 aggaataacc ctatgtgtcc ccttatttat tttatgaatg catcatatat tgcccttata
     3421 gcttcttcaa aatctttgtc ttccactgcc acaataatgt ttatttcgct tgagccctgg
     3481 tttatcatac gaatattaat atttctgttt cctaaggctg taaacaattt agctgatata
     3541 ccgggtcgat acgccatttt gattccgact gtggttataa gactgaggtt tctaaaaact
     3601 tgtacattat ctgttttgca ttttttctta agttcttcaa caatttgatg actgattttt
     3661 tcaacggatt ctgaaggaag aacaattgta aagctgtcga tgcctgaagg aatatggtca
     3721 atgcttatgc ccctttcttc aaacacttcc agggcttttt ttacaatacc cacctcatgt
     3781 gccatgtgct ccttatgaat atagaaaaat gaaaaatctt ttcttcctgc aatccccgta
     3841 ataatatgtc cgtttgcata atcttctcct gcttggtcat ctctgattat taaagttccg
     3901 ggattttcag ggtcgttggt atttttaata tttattggaa tacctgcttg tctcactgga
     3961 aaaactgctt cctcatgtaa aactgatgct cccatatatg acaattccct taattcctga
     4021 tatgttattt tcttaatttg cttaggattg tttacaattc gagggtctgc aactaaaaat
     4081 cctgatacgt ctgtccagtt ttcatatata tctgctccca taacactcga aacaatcgag
     4141 ccggtaatat cggagcctcc tcttgaaaaa gtctttatgc ttccgtccgg atatgagccg
     4201 taaaagcccg gtataattgc cttattgctt ttgcccagta tttctgtcag tttttcatga
     4261 gttttttcct catttaccgt accgtcgtag ttaaaaaaaa taacatcctt tgcatcaata
     4321 aaatcacaat ccaagtaatc agccatcaac aaggcacata aatattcgcc cctgcttacg
     4381 atatagtctt cttcacattt attgtccaag ttttctttta taattttaaa ctcatagtct
     4441 aaatccgttt tcaagttcag ctcttcctta atctgcctga atctgtcttc tataattttt
     4501 aaaataggag catagggtac cgagtacttt atatgtgcat acgtaagata caataagtcg
     4561 gtaattttgt tgtcatcctt gaatcttttg ccgggggctg atactattat atattttctt
     4621 tcatcattgt ttttgattat gtcatatact ttattaaact gcttagagtc agctaatgaa
     4681 gtacctccaa atttagcaac ctttaacatt tcttcctcca aattaattta taactttttt
     4741 acaatatcat aaggaaatat aaatgtcaac gtttttaata cgaatgtatt tatttcgtaa
     4801 acaattataa aaagtttctt attaattttt ctctgccttc aggatgtata tatgaaagag
     4861 gaacatcaaa aatggttttt gctcccttaa ttccttcttt gttcattctg taagcagctc
     4921 ttgcatacga tagaatcacg cttccggtaa actcgggatt gctgtctaat ttaagtgaga
     4981 attccattat atgttggttt ttctcacttc ccgtcatacc gtttcttatt acaagtcctc
     5041 catgggacat cctcgaatga tttttcttaa attcttcttc gcttatgaaa ttaacttctg
     5101 tattatattc tgcaaagtaa tccggcatgt tttttatatc ttcttcaatt ttatttttat
     5161 ctgcacattc atcacaaacc acatagcatt gtcttatgtg tttctctctt gcagtaagtt
     5221 cggggttatc tccccgtctt acgcaattta ctgcatcctc cacaggtatt gtgtattgaa
     5281 ttgcctgttt tacaaacgga atcctgcgaa tagcatcaga gtgtccttgg cttactcctc
     5341 ttccccaaaa ggtatagctg ctgccttccg gcaggactga ctctgacagc atcctcataa
     5401 ttgaaaacag tcccgggtcc cagccgattg aaattatgct tattttgccg gaagaaagag
     5461 caccttcatc aacttttctt aagtattccg gaattttttt atgattatca aagctgtcaa
     5521 cagtattaaa catttttgca tattcagctg cctgtacggg taggtctgtt gccgagccta
     5581 aacacaagac cataacatct attatgtctt tatatttttc aacctcatca acacttaata
     5641 cttttacgtc agtctgagtt tttatcgtat caggctgtct tctggtgaag acagccacta
     5701 attccatatc tgtacttaaa tttaccgcag cttccactcc tctggctata ttaccgtatc
     5761 ccacaattcc tattcttatt ttgttcattt ttccctccgc tgtctttatt tttgttaaaa
     5821 gaaatcttcc ataaggtagt aaccggggct ttttgatgaa agtttcatag ccaagtttat
     5881 agctccgcga gcaaatattt tttttgaaaa agcggtatgc tttatttcta aaatttcatc
     5941 tactccggaa aatataaccg tatgctctcc ggggatggtt cctgccctta tggcactgat
     6001 ttccacatgc tcccttcctg tcgcattttt aatgtcttcc gacaatgttt ttgctgttcc
     6061 gctgggagca tctatttttt tggtatggtg tttctcaaca atgttaatat caaaagtact
     6121 tagctctgca gagatacttt ttaatatttt tctgaaaaca tttattccta atgaagtgtt
     6181 tgaagataac agtacaggaa ttgctttccc tgcttctttt attttagctt tatcctcatc
     6241 gctaaagcct gttgtgcaaa tcaataatgc acatttattt tttgttgcat attcaattaa
     6301 aaaatcaata tttgcagtgt ttgaaaagtc tattactaca tcaggcatac cgtctatatc
     6361 ttgtccaaat ctcccgctcc ttggtgaaag ggagccgatt agattaatat tgtcatatgt
     6421 ttttatctct tcatgcaaaa ctcttcccat agctccgttt attccgcaaa ttacaacatt
     6481 catactccac ctcacgcggt ttctaaagga acgcctattc cgaataaatt caattctctt
     6541 agaagtcttg ctctgttttc ttccgacatc tgatataagg gtgctcttac ctcagagctg
     6601 cacaagttca taatactcat tgcttctttt acgggaatgg gatttgtttc aataaacaat
     6661 gctttattta ttccatttag tgcaagctgg gcattctttg catcatttac atttccgttt
     6721 ctgtaatcat caaccatttt cttcatttct gcaggaagta tatttgcaga tactgaaata
     6781 actccatgtc ctcctaatga cataaggggt actaccatat cgtcattgcc tgaataaacc
     6841 agaaagtcat ccggacatat tctgattatt tccgcaacct ggctgatgtc tccgcttgct
     6901 tccttaattc ctactatatt cttgattttt gaaagctcta agactgtttt tggttcaata
     6961 ttcattcctg ttctggccgg cacattgtaa acaataattg gcagtgatgt actttctgca
     7021 attattttat agtgatttac aagtcctctt tgagttgttt tgttgtaata gggagttact
     7081 atcaataggc cgtctgctcc cgcctcctca caaaactgtg ccttttttac tgcagcttgt
     7141 gtacaattgc ttccggctcc tgcaataaca ggaattcttt tattgactct gtcaattaca
     7201 aattcaacta ctgtgtgata ttcttcatct gtcattgttg ctgcttcaga agttgttcct
     7261 gcaactatta ttgagccgga attttccaca tgcatctcaa ctaattttcc taagacctca
     7321 taatcaattt ctccgtcagc aaagggggtc accaatgcta ctccgcatcc tcttactaaa
     7381 tcattcatct tcttactcct ttaaattatt ttttataaat aaaataattt catgtcgctc
     7441 cctatgacct accatccgtc tgtacctcgc cggagcttgt acaaatattg aggtataaaa
     7501 aaagcgacat aagagcaaaa gggctctcag acgcttaaaa aacatcgaac cttctgccca
     7561 acatattaca gcgctctttt ataggattgg gatgcatcct ataaaaacag tatcacatct
     7621 attaaatgtg agcccaggga ttaacccttc ggcgaatcac cctttcgaag aaaagcttct
     7681 actcttgatc atcgcgacct ctgtaatatg cccatgaaaa tatttaattt ctccaagtat
     7741 aatactgttg acattacatg tcaagcataa tattatattt tttataatgg cagtagcttg
     7801 taaatatgga caaaatttca agcaattctt gcaaatagtt ttttctacaa atttttaatg
     7861 catttttaag aatattatat tataatcaat actgtcggta tacccggctc ctaatgagga
     7921 cgggtaaaaa ggaatgtcct cgaggaaagg agatgaagaa tatgttgtgc tcaatatgta
     7981 aaaagaatgt agcagtaata tatgttaata aaattgttga aggtaaaatg cagactacag
     8041 gtttgtgcat tccttgcgct aaaaagcagg gtatagcacc tatggatcag ataatgagac
     8101 aaagcggcat gaaacctgag gactttgata atataaacaa acagcttact gaaataatag
     8161 gagatatgga tttagaaaaa ctatccggag atatgtctga aagcagtgac ggcattaatt
     8221 ctttaatgaa tttaatgaat aatgctttta cgggaaacac cagctctaca agcaatgaat
     8281 ttattccgga ggatgaggat gcagactata ccgggccggg ttccggaaca ggtcctaaaa
     8341 taaaggaaaa agtaaaacga aaaaaattaa aatatcttga tacttatgga ttgaatttga
     8401 cagagcaggc acgatttggc gaattagaca ggataatagg cagagagaga gaaattgaca
     8461 gagttgtgca aatattaaac agacgctcta aaaacaatcc cgttttaatc ggtgagccgg
     8521 gtgtaggcaa gactgcgata gctgaaggat tagcggtaag aattgccgaa aaaaaagttc
     8581 ccgtaaaact tttcaacacc gaaatttacc tgcttgactt aactgccgtt gtggctggca
     8641 cacagtttcg aggtcaattt gaagctcgca tgaaagctat tattgatgaa gccaaaaaag
     8701 caggcaatat agttattgta atcgatgaac ttcataatat tgtaggtgcg ggagaagctg
     8761 aaggcggagc tatgaatgcc gctaatatac ttaaaccggc acttgccaag ggtgatgttc
     8821 aaattatcgg tgccacaaca ttggatgagt acaggaagca tattgaaaag gatactgcac
     8881 ttgaaagaag attccagccc gtaatagtag atgaaccttc catagaggat tccatcgaaa
     8941 ttataaaagg aataaaagat tattatgagc agtttcacaa tgtaaaaata accgatgaaa
     9001 ttattaggca ggcagtttta atgtctgaaa gatatattac cgacagatac cttcctgata
     9061 aagcaattga tgttatagat gaagccggtt caagggcaaa tttaaagaac aaaggattgg
     9121 ttgaacttga agctttaaaa gcagagctta gcagaataag agacgaaaaa gaagaagctg
     9181 cagaaaatga tgattatgaa agagctgcag agcttaaggt acaagaaatc agaattgaat
     9241 caaaattaaa agagttatcc aatgaatacg agaatattct tttaactgaa gaagatattg
     9301 cgtatgttat tgaagcttgg actaaaattc ccgttcagag aataagcgaa gctgaagccg
     9361 ataaactaat acatttagag gaaagacttc acaggagagt tgtaggtcag catcctgcag
     9421 ttacaagcct tgcaaaggct ataagaagaa atcgttcggg atttaagaaa aagaagaaac
     9481 catcttcgtt catatttgta ggtcctacag gtgtgggtaa gaccgaactt gcaagaacct
     9541 tggcttatga actgtttgag aatgaagatg caatgataag gcttgatatg tccgaatata
     9601 tggaaaaaca cacggtttcc aaacttatag gagctcctcc cggatatgtc ggctttgacc
     9661 aggcaggatt cttaaccgag aaagtgcgaa gaaaaccata ttcggtaatt ttattggatg
     9721 aaattgaaaa ggctcatgaa gatgtattta atatattgct gcagatattg gaagacggaa
     9781 ggcttacaga caatcaagga agaaccgtaa attttgaaaa tacggtaata attatgactt
     9841 caaatgcagg aacatctatc aaggctaacc gaatcggttt tggcaacaca gaatatgaag
     9901 caatggaatc caaggtaaag gatgctctga aacaagcatt caggcctgaa ttcttgaatc
     9961 gtatcgatga tataatagta tttagcgaat taaccaagga agaattaagt cagattgttg
    10021 atttaatgct taaggatgtt attaaagaag ctgaagaaaa gaaaattact ttgacggtta
    10081 ccgataaaat gaaagaacat atacttgaaa aaggctataa tacaaagtat ggcgcaagac
    10141 ctttgaggcg ggcaatacaa aagtatgttg aagacgaaat ctcggaagca tatttaaaag
    10201 gtgtatttaa ggaaaattca ttggttactg ttgatgtaaa tgatgaagga aaaactgtac
    10261 tgacgattat ttagtcacct gcattttgct atccgctgat acgttttggc atcctgtgtt
    10321 taagcgaagg aactcatgag attcttcact gcgttcagaa tgacaaaaaa aataaagggg
    10381 aacacctttc aaaaggaagt tcccctttta ttattgttat ttgtttacaa atccgattcc
    10441 taatactccc ggacctgagt gggttccgat aacagctcct atttcctgct cgattatatt
    10501 ttttatttta tatttatctt caattgattc tcttaacatc tttaattgct cgtaagcttt
    10561 tccgtgaaac aggagcactg ttttgtttga taaatcatag ttgttctttg ctatccattc
    10621 atcaaaccat tttacagctt tatttttacc tctaattttt tcaattacat taatttttcc
    10681 atccttaatt tcgataatcg gcttaacgtt taacagagtg cctacaaccg cttgtccctt
    10741 tgagagtctt cctccctttt caagatactt tagggtgtca agtgcagcta cggcaacaac
    10801 tttttccctt agtgcctcta attctttcac tatttcttcg atatccttgt tctgctgtac
    10861 taattcgatt gcttccaata tcagtgcaaa tgcaccgaga cagacacttc ttgaatcaat
    10921 tatgtaaata tcatcgctgc caatcatatt cttggccata caggcggaat catatgttcc
    10981 tgaaaattcg gaagcaagaa aaagccccaa taccttgtct ccttctaata aaatatcact
    11041 gaatacttcc ataaatgttc ccggtgacac taaggtagta gtaggtagtt tttctgcagt
    11101 ttcaagtttt tcaaaaaatt gttttgaatt caattcaact ttgtctaaaa atgattcatt
    11161 ttcaaaattg acagtcaggg gaaccatttt tatattatac tttttaatta tttcatccgt
    11221 tacgtccgaa gtactgtcga ttacaattct tatacccata ttaacctctt ttcatagtca
    11281 taaattttgt tctatagtta ataccatcct gcatatttat atttttcgct gaaaaatttg
    11341 tctatgtttg taagcaccct tatcaaaact tcttcttctt cgctgtttaa ctgttgtaat
    11401 gcactaatta ccaaatcttt atggaagtct tcatgagatt tgtatgctgc tttacccttt
    11461 tccgtcagtc ttatttttac aactcttctg tcttctgatg agcggcgtct ttccacatat
    11521 ccttttttaa taaggttgtc tatggcagtt gttaatgtac cggaagtaac ctttacagcc
    11581 ttggcagtat cagacatggt gcgttcagtt gaatatccta tgttttcaat taaatgcatt
    11641 tctgttatgg ttagatcttt aaattcccct attttcaggc atttttcttc gatatacaat
    11701 atgtttgcaa aaagcctcac aagtatttca tttactgttt catgattact cattaaacca
    11761 ccccgattac tttgatattc aaattatatt tgtaaaaata ttttttgtca agccttttta
    11821 tgatgaattg tatatctcca gagaaaatct ttcgcttcct ctgcataatc aatatttatt
    11881 cttttgctct tttctacctt aaagtcttta aagccgtcat cttcaacata aatttcattg
    11941 cctgtcaaat ccgttccatt atgttctatt gttattttta aagcattgca aagttttccg
    12001 ggaccgtttg aaatgttttt tttctgatac tttgtaatat catccaaatg ttccttatat
    12061 ctgttaaaac acatttcttc tttattgatt accggttcca ctgcccttat taaaacagca
    12121 ctgcaggtcc cttcttcttc tgttacaaag tttaaacaat aatacatgcc gtaaataaaa
    12181 tacacatatg catagccggg aggtccaaac ataattttcg tacggggtgt tatttttcct
    12241 ccataaccgt ggcttgcctt atcaattgca gctatgtatg cttctgtttc aacaatcctt
    12301 gcctttatta ccgtattgtt atactttctg actaaaattt ttcctattaa gtcttctcct
    12361 acaatacgag catctctatt ataaaaattt ctgttaagct tcataattat tacggggaca
    12421 ttcctttaaa tgcttacctt caaagactat ctttcattta aaggtgtgtc ccccttcctc
    12481 tatattaaat aatcttttaa aattctcctt ggttttttct gctacctcat caattgatac
    12541 atttcttatt ctagctattt cttcagcaac atatttaaca tatttaggct cattcctttt
    12601 tcctctatat ggcaccgggg ttaaataggg agagtcagtt tcaattaaaa gcttatcaaa
    12661 ggggacttct tttgcgacca atttgggtac ccttgcgttt ttatatgtta caggtcccgc
    12721 cagagatatg taaaatccaa gctttataaa ctctttcgac aattcaacgc ttccggaata
    12781 acagtgaagt attcctcttg ctccgtcata atgttcttcc ttcataattc tcaaaatatc
    12841 ttcatgagca tctctgtcat gaacaatgta cggaagattt aattcttttg ctaagcgtat
    12901 ttgctcacgg aaccattttc tctgtgtatc cctgtctgaa ttgtcatagt agtaatcaag
    12961 acctatttcg cctattccta ttaccttttt attttttgcc atctctatga attcattcat
    13021 atgctcatca ctcatatatt ttgtatcgtg aggatgacat ccaacagcgg cataaataaa
    13081 ggaatactta tttgccatta atagagcttc ttttgaagat tgtaaatcgg cacccggatt
    13141 taatacagct tcaacatcat tgtcatataa tgagctaata agattatccc tgtctgtttc
    13201 aaatatttta tcatcaagat gtgcatgact gtctataaat ttacttacca cttttcttat
    13261 ccttcttttt agtattcctg taggtttttg catgttcttt aaatttatcc aattgagggt
    13321 cattctttaa aaatgtaaga tggatatttg aatccttatt cattacgcta taaattatgt
    13381 taaccttaac tttgcctttt tcatcaggtt tagggtaatt tataccttta atatcttccc
    13441 atcttatgag gtttcctctt ttgtgtattc ccaacgttga aatcattgtt ttcttggaca
    13501 aaggtatata cagtgtaatt atcagtatag gaagtactcc cattatcaat acttgctctg
    13561 cagcatatgt ctttgtttct atcaatccac gaatcattat gcccgatatc aaaatcatta
    13621 gtgccataag taatctgctc atcaagtgta cccttttatc gtcttcatcc aagaaaagca
    13681 aagcttccat gttttctttt ttatattgca ttctgttttt ggtgttttta aataaataat
    13741 atattaaaac taaaaatatt agcggataaa atattgaaga aactgctgcc ataggtatgt
    13801 acctccttta attttctgta ttagtgtaac ataattttaa ttatgtttca agatgaaaaa
    13861 gcaggggaca ttctttcaaa actttacctt caaagtcttg actttgttaa ggtttgtccc
    13921 catacatatg acaaaaaact gtctcgttga gacagtaagg ttatcatatt cttcattgcg
    13981 ttcagaatta caggctgctt attcagaagg ataatgtgaa ggtcatactt agtatttaat
    14041 tatttttttc tcaagccagg aagaaaaaac atacataatt gctgcaatca ctcctaatac
    14101 aaatactccc atcattacaa ggtctaattt aaatgtttgg cttccataca atatcaaata
    14161 tcctattccc gcttttgaaa ccaagaattc tccaacaatg accccaatcc atgataatcc
    14221 tatatttact tttataacgc ttaaaatatt tggaatgtta gcaggtataa ttactttttt
    14281 taaaatttga aacttgtttg ctcccattgt ttttaagaga ataattttat ccttttcaac
    14341 atttgaaaat cctgtgtaca atgtcatagt ggtaacaacg attgaaatgg atgcagccgt
    14401 agctattatt cctgaatatc ccattcctac ccacaagatt attatgggag ctaaggccgt
    14461 tttaggaagg ctgtttaaaa taacaatgta agggtccata accttagcca attttctaaa
    14521 ccaccacaaa attattgcaa ttaataagcc gccaacagta cctgcaaaaa atccgcctat
    14581 tgtttccatt gtcgaaatga ataaatgttt aaacaggctg ccgtcatcaa caagcttcat
    14641 aatcagacca agaattgctc ccggactgct tgttagaaat gtatctatta aatttttcct
    14701 ggctgctact tcccaaaata ttattccaaa cacaaaaatc aagatttgtg caatcctaat
    14761 aaagtttttt tccctattta ttaacttcaa atattctttg tgctcttctg aaatgtttgc
    14821 aaccggctca aacttctttt cccacatcta aatccctcca tatatttctg aaatattcac
    14881 caaaagccaa ggtatttctg catttcatag gtgttctttc acctggacac tcctcaaatt
    14941 ttatttcata ttccttttta attttagccg gcctttcagt gaagactata actctgtccg
    15001 aaatgcttac tgcttcagga atgtcgtgag ttacaagtat ggtgcttatt ttttcatttc
    15061 ttaaaattgt ccccacttcg tcacctattg aaagtcttgt ctggtaatcc aatgccgaaa
    15121 agggttcatc caataaaagc agctccggct caattgccaa agtgcgcacc agagcagccc
    15181 tctgtctcat tcctccggac aattggctgg ggtaatggtt cttaaaattg gtcaacccat
    15241 atttttcaag cattcttttt acttttatct tgttttcttc attatccatt ttttttactt
    15301 ctaaaccaag aagacagttt ttatatacat ttctccattc atataactgg tctttttgaa
    15361 acataaaacc aatactgtcg gtattaacaa taacttcccc ctcagacggc ttctccaatc
    15421 cggctataag cgataaaacc gttgactttc cggagccgga aggtcccact atagaaacta
    15481 tctccccttt tttaacctta aatgaaatat catcaattgc ctttgtctcg ttatttattg
    15541 tatgaaatct tttagagaca tttctcagtt cacaaatata taccatatct acctccgtca
    15601 tactgtgact ccttacatca tcttattcaa aactgattaa taagttaatg ataataatta
    15661 aaaaacctta gtttcaaagt tttataaaca gtaataaatt tctaaaatta agtaaaacta
    15721 taataaaggt ggtgaaatta tgtttaagaa gttaacaaac aaattgaaaa tgagatatag
    15781 aagatctgcc aatcccggga atgtaaatat tccgggtgaa tttgaagtgc aagagcgaaa
    15841 aaaagacgtt gataacaata aaaaataaga tgccctaaca aaattttgtc atcctgatag
    15901 gcagcgaagg atcttaagtt atgagattct tcactgcgtt cagaatgacg atttgagcat
    15961 tttttatagc agcatcttat ttatatttat cacatttttt taagaattgt gtcaattcat
    16021 ctattttatt tagaacctca tcgtttaccg catgttcaat tatttcggtt tgctccaaaa
    16081 tattttcctt tactcctatg cattctaaaa attcaaaaat tatccggtgc ctttccaata
    16141 atttgctgcc tacttccctt cctttttcag taagtttaat aaatccgtac ctttcatagg
    16201 ttacataatt ttcctttgcc agccttctta tcatttttgt ggttgacgga ggctgcacat
    16261 tcaaggctga agacagctcg cttactctga cttcatctga tttttcagac aatctgcata
    16321 tcatttctat ataatcctcc attgaggaag agaggatttc ttcatttttt attttataac
    16381 cttttagtgt ataaaactct tctttcataa tgcctcctaa ttgtgtcaaa attcgagcaa
    16441 tgtcatatta taatttatag aatgttagct ttggctaaca tgttctaaaa aatattatgc
    16501 agttatattt taaatattac aggagtttaa atatgaaatt agctgatgtt ggtgttggaa
    16561 aaacagtaaa ggttaaacaa aactctgctc atgattattt aaaggaaaga tttcttgctt
    16621 tgggacttac aaaaggtgca gatgttgaag tattaagaaa aggtccaaaa aacaatttaa
    16681 ccgtttacag aataagagga gcaatgattg cccttagaaa tgaagaatca agtttaattg
    16741 aagttctata gggggtgtca gcatgggact tacagcacaa tccgtaaaat tggaatcatt
    16801 aagagatatt tatgaaatag aaaaaaatga taaacagtat gtagtagcct tggcaggaaa
    16861 cccaaacaca ggcaaaagca ctgtttttaa ttatttaacg ggacttaatc aacatacggg
    16921 aaactggccg ggtaagactg ttgtttctgc aagaggccag tttaaataca aaaataatga
    16981 atatatacta attgacctgc ccggctctta ttcattgttc accgcttcaa aagatgaaga
    17041 agttacaaga gattttctat gttacggcaa ttatgatacg gttgtggtag ttgctgatgc
    17101 cacgtgtctt gaaagaaatt taaacttggt ttttcaggtg tcggaactta cgaataaaat
    17161 aatactttgc attaatttaa tagatgaagc aaagaaaaaa aacattaaaa ttgacaaaga
    17221 aggtcttgaa aaagaattaa aaataccgat tgtactaact tccgcaagga atggtcaggg
    17281 tatgaaggag cttaaagatt taattgaaaa aacatcacaa atcattgaag aggaaactga
    17341 aaaatcattg tattttaatc aggatgaaag tataagagaa aaaatttcag aagacaacta
    17401 taattatgcc aataaattaa aacaaaaata tgtaaaggag gatgttaaca aacttaacag
    17461 agacaaaaaa attgatgata ttgtaacttc aaaaaaattc ggaattccca ttatgcttgc
    17521 tctattgggg ctgattttct ggattactat tcaaggagca aatatccctt cacaaatttt
    17581 agctgattta ttgttcggtt tacaatataa attagacaaa attcttctaa tcttaggtgt
    17641 aaatgatttt ataagaggtg tttttatcga tggtatgtat aaaagccttg catgggttat
    17701 ttctgtaatg cttcctccta tggcaatatt tttccccttg ttcactttat ttgaagactt
    17761 gggttatttg ccgagggtag catttaattt ggaccatctg tttaaaagag caggcgctca
    17821 cggtaaaatg tgcctttcaa tgtgcatggg gttcggatgc aatgcagcag gagttatagg
    17881 atgcagaatt attgactcgc caagggaaag attaattgcc gtcattacca ataatcttgt
    17941 tccctgcaac ggcagattcc cgctgattat agtattgtca gcagcatttt ttgcttttac
    18001 cggctcattt gttgacagca ttatacctgc tttatcaata accttgatag ttctaatagg
    18061 tgtatttgca actttgtcag tgtcatattt actgtctaat actttattaa aagggattcc
    18121 ctcaaccttt actctggagc tgcctcccta cagggctccc caggtaggca gaataattta
    18181 tacatcactt atcgacagaa caatttttgt gttgtctaga gctgcagttg ttgcagcacc
    18241 tgcaggagca attatatggc ttttagcaaa tatttatgtg ggtgagttaa gcattctgat
    18301 tcatatggct aatttttttg aacccttagg aagagcaata ggacttgacg gattcatact
    18361 tattgctttt atagccggtt ttcctgcaaa tgaaatagta attccggttc tattgatggc
    18421 atatatgtcg acaggtactt tgacagattt tgaaagtatt gattctctaa gacaaatatt
    18481 gatatcaaat ggttggactc aattaaccgc cataaatgtt atgttgtttt ccctgcttca
    18541 ctggccctgc tccacaacct tattgacaat aaaaaaagaa accggaagtt tgaaatggac
    18601 tttggcagca tttataatcc ctaccggtat cgcatttttg gtatgtttta taacagccaa
    18661 tttatacaga ataatttggt gacgctgggg acgtacacta actgtcccat tcactacaat
    18721 caaataacaa aatcaaaagg gtttgagtat ttcaactcaa accctaaact ataacagcgg
    18781 gacagtagtg tacgtcccca ctgtccctta actgtttatt gcattttcaa ttcttcttct
    18841 aaccttttct tctcccatta tctgctgcaa ttcccataag tccggtgatg atgcgcggcc
    18901 tatgattgca attcttatag cggtacttac atctcccaca tgtcctttgt acatatccgg
    18961 gtttttcttg taatcctttg gctttgcagc atatccgttt tcagatgcaa ttactcttat
    19021 cttttcaaac cactggcttt gatcgtcacc gtggtcgtag gtacttaagt aagctttaag
    19081 aagtttttta gcttcttcac tatctatatt ttcaggataa ctatcaacaa ttttaaaata
    19141 ttcatcaaag taataactta tgaaatccag tatttgctca caatacatca aatccttacg
    19201 aggattgttt ccatctcttc cgacagacaa gagctttata acttcatctt tatttgattt
    19261 caaaagttca taggcttctt ttttgtactc atttgcccag ttaagcaaaa attcatatat
    19321 ttcagctgca ggaattttta ataaaacatc cttgcaaatg ttatttaact tgtctaaatc
    19381 aaataatgct cctgaattgc tcatttttcc caaagtgaat tgaaaatcat caatcgggtt
    19441 ttcgggattt ttcattctcc actcttcaaa gtttgaattt aaaatggtaa gaaggtattc
    19501 tcttacagct ttcggatgat aacccaattc cctgtaatat cccaacccta attcagggtc
    19561 cttcctcttt gaaagctttc ttttggctcc attatccatt ttcattaaat gcgccgtatg
    19621 gcaatataca ggtgtttccc aacctattgt tttaaatatt tcataatgga taggaagtgt
    19681 ggacagccat tcttctcccc ttacaacatg agttatgccc attaaatggt catctaccac
    19741 gtgagcaaaa tgatatgtag gtataccgtt cgttttcaga attacaatat cctgataatt
    19801 ttcaggcatt attaaacgtc ctcttatggc atcgactatt tcaaatttcc cttcttctgt
    19861 tccttcagag cgaaatctaa taacaaattc ttcattgttg tttaaatgct gctgaacttc
    19921 ttctaatgat aaatttctgt ctctcgccca cttaccgtaa tatcctgtat ttacattttc
    19981 ttttgcttgc tctttcctta attcttccaa ttccccttcg gtgcaaaaac aagggtaagc
    20041 cctccctatt tcaaccaagt gtttagcaca gcactgatat atttctgctc tgttgctttg
    20101 aaaatatggt ccgtaatctc ctgtttcccc ttctattcct gcgccctcat caaatttcaa
    20161 gtcaaagtat tcaagggatg ttattattgt ttcaacagag ccttcaactt ttcgtttttc
    20221 atcggtatct tctattctaa gcaaaaaaac accaccgctt tgatgtgcca aacgttcatc
    20281 cacaaatgct ccgtaaagat tacccaaatg aataaaacct gttggacttg gtgctaatct
    20341 tgttacttta gctccttcag gcaattttct cttaggatat acttcatttt cataatattc
    20401 aatagtcttt gttatatttg gaaataacaa ttctgctaat tttttataat ccatctcaaa
    20461 cctccttgta cgggacattt ttttaaaggg acggagttgt tgtcattatg acattacccc
    20521 cgtccccttg tcatctttct ttaataaccc ataggggagt cgaacccctg tttccaccgt
    20581 gagagggtgg cgtcttaacc acttgaccaa tgagtctaaa attgtgactt ttggttagtc
    20641 ctaagtatgg aacatccttt ctcaaaggct atcgcttaag aaggtgtacc ctatacttca
    20701 gtaattatat aacaacctgt tatctttgtc tatatttttt tgggtaggta ttcaccaaga
    20761 tatttctatt attataatga tacccggttt ggatcattta ttgattattt ttctggacaa
    20821 tcaccacagc aatgtccgcc cacatcccct tctttaacca tcaatgtttt attattcggt
    20881 acttttgtga atcctatatt ttctaatatc ttctctatac tttcatctgc ttcgatataa
    20941 gttttttctg ctcctattgt aactgctttt ctaacaagca gccttaccag caaatcctca
    21001 tatcttaatc ctgtaaattg tttttttata cataacttat caataaagta tttgccttct
    21061 ttaaatgaca gtcttgctgt tacggcaggt acatcatctt catacaaaac agcattaaaa
    21121 gcaaattcat cataaaaatc cgtaatattt tcttctctgt tttcttcagc aaatacttct
    21181 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcaaacgc attgaatgta
    21301 ccaaaagaaa gaataaaact ttatgaaacg ggagaagaaa taccttctga tttttatttg
    21361 tcaagctttg tacaatattt caatatttca aaggaagaac taattgaatt gattaaataa
    21421 tcttaacaat aataatgttg aaataattat tattatatgt taaaataata acataatcta
    21481 agcttaagga tcttctaaga gcaaggaata cacttatagt attaattatt attccttgtt
    21541 gtgcaaggaa ttttttattg aggtgcatat ggataaaaga atcggagtta taggcattgt
    21601 aattgaagat acatcccaaa ttgcagaagt gaaccgtctc ctgcatgagt attctcatat
    21661 tattataggc agaatgggca taccttacag ggagaaaggt cttaatgtta tctccattat
    21721 agttgacggc actaacgatg atataggagc tcttagcgga aaattaggca aaataaaagg
    21781 actcagcgtc aaaagtgctt tgtccaaaaa cattaggagg taatatgaaa aaaattagtt
    21841 ttttgttggt ggtacttatt gtgttaattt ttacggcagg ctgcaacaat gaacctgcag
    21901 agacaccgac acccgatgaa aatgcagaag aaacacccat gcctgaagaa ccggcagatg
    21961 ttaaaccggt aaatgttact attgcagggc ttaaaggtcc cacatcaatt ggtatgatta
    22021 aaatgattga tgaaaaggca cttaaaagcg ataaatacaa tgtagaatat atccaggaat
    22081 cagcacctga ttcacttacg ggtaaaatta taaacggcga tattcaaata tcatctgttc
    22141 ctataaattt agcatcggtt ttatacaata aaactcaggg aaaggttcag ttgttagccg
    22201 taaatacgat tggaaatcta tatatagtag gaaccgagga tataagctcc gtttcacaat
    22261 tggaatgcaa gacgcttgga atgagcgcaa aaggttcaac tcctgatttt gccatgaatt
    22321 atattttaaa gcaagcaggt cttgaaggta aaacagagtt aaattatgca cttgaccatg
    22381 caactcttgc acagtccgtt atagcagaag atacaagggt tgcgcttttg cctcagcctt
    22441 ttgtaactca aactactctg aaaaatccca acgttaaaat gcttatagat ttaaatgaag
    22501 catggaatga tgcaacgggc ggaaaaagcg aattatttac gggatgtatt ataataaaca
    22561 aagagtttgc agaaaataat ccggaatttg tagatgaatt cctgaaacaa tatgaaaact
    22621 cggttaactg ggtattggag aatcaaaagg aagcatctgt attaaccgca aaacatgaaa
    22681 ttatgcctga tgccgtattg gttgaaaaag ctcttcctta ctgcggcata acatttagaa
    22741 aagctgcaga agcaaaagaa agcttaaata atttttatca aatattattt gattcaaacc
    22801 ctgcttcggt aggaggaagc atgcctgatg atgaattcta ttttaaggaa taagaacaaa
    22861 ttattgatta tacttttttg gattttcgtt tgggagatac tatcgctgat aatagctcag
    22921 gagatatatc tcccttctcc tcatgcagcg ttttcttctc tcatgaattt gttgatttca
    22981 aaggatactt acattactat atattacagc acttacagga cattgaccgg atttttttta
    23041 tcctgtgtct ttggcatatt tttaggaatt ataagcggca ttcataagac tgtatacaat
    23101 ttgcttaacc ctttgatagt aattattcga acaatccccg taatgagtat tataattatt
    23161 gcaattatgt ggttcaagga taccaatgtt cccatttttg ttgcattctt aatgtgcttc
    23221 cctataattt ggactaatac ggtttcaggt atcctatcaa ctgatgttaa gcttcttcaa
    23281 atgtgcgaag tttataaaat aaaaaagtcg aggataataa gttcagtata tttttattct
    23341 tctctccctt atattaaggc agggatgatt tctgcactcg gaataggctg gaaagttact
    23401 tccgcagcag aagttctaag tcttcccaaa tattcaatag gtagattttt gtacgattca
    23461 aaggtatatc ttgaaattcc cgacttgttt gcctggacaa ttattatagt ttcactaagt
    23521 tttctgtttg aaagcctgct taaaaccatt ttcaaattcg gagataaata tgattaaatt
    23581 aattaatatt tctaaaaagt atgataacct tatattattg aaagatttta acattgattt
    23641 tgaaaaaaat aaaataacat gcttgtttgg tccttcaggt gtaggaaaaa ctacaattgc
    23701 aaatattgtg gcaggaattg tacctgttga tgagggtcgt gttgtcggta ttgacaattc
    23761 attgttttct tatgtttttc aggagccgcg tcttcttaaa tggtacaatg tatatgaaaa
    23821 tattgatttt gtattaaagg atgtttataa acctgacaaa agggcggaaa ttataaacag
    23881 ttatttggaa atggttgaat taagcgaata cagaaattat aaaatagatg cattaagcgg
    23941 cggaatggcg caaagggtat ctctggcaag agcttttgcc tatccttctc atatacttat
    24001 attggatgaa ccgtttaaag gtcttgacaa taaattaaaa ggcgaaatga gctctttatt
    24061 tttaaaaatg tggaacctca gccaaagaac ggtaatcttt ataacccacg atgttgaaga
    24121 agccgttgat ttatctcata ttatttacac tttaaaagac agacctgtta atattattga
    24181 cagattaatt gtaaagaatt atgcaaataa taaagaagaa ataaaaaatc tgcttcttaa
    24241 ccattaggaa atcggctgta ttgatatata tacttttttg gcagataaag tctcaaaatt
    24301 atgtgtgatt aaatcactga ttattttttt taatcatata agattaagca aaaagcgagg
    24361 aggatttatg aatatagaaa taacagacag tgcaaaagaa aagctcttgc tcatgaagga
    24421 aaaaaatatg cccataactt tgatgaaata cccggtggga tgagcaggct ttaaatataa
    24481 aattgtttcg gcgaaacaaa cggaagatga taacttgtat aatgtagacg gtttagatat
    24541 catagtatcc aaggactgtg aagttgggtt gaagggtgta aggattgatt atggcggtct
    24601 cctcttcaag gattttatag tgtctccgat attctcataa aaaaagtaat cctgcctgaa
    24661 ttatcaggca ggtctttttt attcctatta tgacctaacc ccatttcttt gactaaaata
    24721 ataaacttcg gattagtcac atttacagaa tattgcaacc aacacaagga agaaccacaa
    24781 taaagtatca aattcaatat cacaatcgct ctgatccaca ataataacta ataacaaaaa
    24841 gaaaaacaac aatgatgaat caattccgcc tgttcccata acacggtctc tgtctcttct
    24901 tatacagtct ctccttcccc tatcgccgcg tacgcaatcg ccttgtgtgt ttacatcagc
    24961 ttttgcttct acaatatttt caggtttatt acactccata tttacctccc aattacgaat
    25021 attacgataa tttattatat tcatttgaaa tatttatgtt aatgattatt gtcttattga
    25081 attttttgaa aatatgcata atatagaaaa attttgaaat aataatttta tattcataaa
    25141 tgaggaggat aataataatt ggataaattt gcattgtttt tagtaattat cggagcaata
    25201 aattggggac tgattgcatt atttcagttt gacctggtag ctgctatttt tggcggccag
    25261 acagcattat tcagccgatt ggtatacggg ctggtagggt tggcaggatt gtactgtatt
    25321 acattattat ttaatgaacg tgaaagagtt gactaaannn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnncct gcaattgaaa gtttttttat ttttaacgac ggtgagccta
    25501 tataggagcc tcccggcaga ccaaatttta aatccgagcc tgtttcttct atatttttaa
    25561 gcatttcaaa gtagtttccc gcaactgtaa tttgctccac aggtcttgaa atttttccgt
    25621 tttcaattaa aaaacccaat gctgccaatg aaaaatctcc cgatacggta tttaagcctg
    25681 aatgcagacc ctgaagttca gtgatgagta ttcctttgtt tatgtctgta atcatttcat
    25741 ttaatctcct ttctcccttt tctacataaa agtttgaagg agatatgcct aatgacgatt
    25801 tataggagcc cttgcttgca tttcctgttg tttcaacgcc gtcttttttg gcagttttta
    25861 aattatgaaa ataagttttt aaaacacctt tatcaacaac ctttttatat ttgcatgcaa
    25921 ccccttctcc gtcaaaggat ttggatgcaa gaccgctttc catataaggg tcgtctatca
    25981 aagtgaattt gtcgcttgct attttttcat ttaattttcc ttttaataag gaaagattct
    26041 tttgcactgc ttcagctgag aaaattccag taaatgcttc caataaatca gctgcagcgg
    26101 aatttctgat aatcacattg taatctcctg attttaccga ttttgcccca agaagagcca
    26161 atgcttcatc tacggcttcc tttgcaatat cacgggcatt aaatttatta aaatcattcc
    26221 ctgtacggta tgccataccc gtctttatgt cttcaccttc ttttgcaaca acaactatgt
    26281 aggtatatgc aatattgctt ttatcttgga ggaaaagacc ctttgtattg gacattatta
    26341 tttctccgta accgtcgccg taaacgcata cttcaacaga ttcaattctt ttgtctaatt
    26401 taaatgcttc ttcttcaaga cttttaacaa attcaatttt cttttcttca cttacatttg
    26461 acaaagccgg attgaatgaa tctactttta tatactcctt cgagccttca aatataaact
    26521 cttcatcgtc cgaatctatt atgacggcat tttccaaaac atctctaacc agttggtcta
    26581 ttgaagtttc atctaccttt tcggtatatg aatatcccat tttacccttg taaacccctc
    26641 ttaaggaaag tccttcgctt tccgacaaat tatacgtttc aagctctttc tgaaaaacct
    26701 tcaatgtaag gctacttcct gcagaataat aaacttccat gtcattcagg cccttttctc
    26761 tgcctttttg aaatatttta tcaatcaatg atttcatttc cattattact caccttccct
    26821 tccgcctact gttaattccg aaaccctaat cataggctgg cctacatttg ccggtatgct
    26881 tccgcttacg gaaccgcaca taccctggct ttgaagcaga ttgtttccta ccatatcaat
    26941 tttattaagt acttccatgc cttttcctat cagcgtagct ccccttacag gctcttttat
    27001 tttaccgttt tcaattatgt atccttccat tactgcaaag ttaaattctc cggtggcagg
    27061 gttaaccgag cctccgccca tatgttttgc ataaagcccc ttttccgtat tagcaattat
    27121 ttcttcaggc gttgaactgc cgttatctat aaatgtattt gacattcgtg atgtcggcgg
    27181 gaatctgtag gattgacgcc ttgaggagcc tgttatatcc atattcattc ttcttgcatt
    27241 taatttatct atcatgtaac ctttaagtat cccgttttct atgagcacat ttcgctgtga
    27301 ttgagttcct tcatcatcta tgctgaatga gccccactca ttgggaattg atccgtcatc
    27361 tatggctgta actataggtg atgcaatttg ctgtccgatt tttccggcaa atacggaaag
    27421 gccttttgca accgatgttg cttctaatcc gtgaccgcat gcttcgtgga aaataactcc
    27481 tccaaatccg ttatcgataa ttacaggcat cctgccgctg ggactgtact tggctttaac
    27541 cattgttacc gctattcttg aagcttcctt ggcataataa tctatatcaa gattttcaaa
    27601 aaattcgtag cctttgcttg ctcccggtgc gaaaaatcct gactgcattt caccgtcttt
    27661 acttgctacg gaagaaatcc cgagtcttga gcgaaccctc gtatcttcct tccacaatcc
    27721 ttcagaatta gccaccatta tattttgtat ataatcggtg taatttatag aaacctgtgt
    27781 aatcagttca tcataattaa aagcaatgtc atatgccttt ttcattactt gaaccttttt
    27841 agttttttcg accttggacg ggtctatttg tatgatattt atatttttaa catctgactt
    27901 tgtcagattt attgttaagt caatgatgtt tccttccacg gcttgagcag cttttttagc
    27961 tgtttctatt aaagtgtctt catcagactt attcgtatat gcataaacac tgttaaatcc
    28021 tttgaatatt ctgataccta cgccataatc tcttccggac atggtgcttt ccaccttgcc
    28081 gcctatcata acaataccgt tgttagttcg gtcttctaca aaaatttcgg cgaaatcacc
    28141 gccggtagat aaagcagccg taagcgtatt ttcaatcagt tttctactta acacataatc
    28201 actccttttc gtctaatatg agattatttt accatacttt cctaaaacta ttaataatt
//
LOCUS       DAOPUE010000013        28113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1652_length_28113_cov_9.854266, whole genome shotgun sequence.
ACCESSION   DAOPUE010000013 DAOPUE010000000
VERSION     DAOPUE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 28113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28113
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_28113_cov_9.854266"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1144)
                     /locus_tag="PKH42_02350"
     CDS             complement(290..1144)
                     /locus_tag="PKH42_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOA19299.1"
                     /translation="MNDLISIIKKNFHKFSRGQKLIAQFIIDHYDKAAFMTAAKIAET
                     VDVSESTVVRFASALGYSGYPEMQKALQVLIKNKLTTVQRISLNDDVNDKLKLHKRNL
                     KNEMNNLRYLYDHFDMEALDKATELILEADRVFVLGLRTSSTLSNYLGFYLDVILNNA
                     KVLNNSGVNSLYEEIIRIKEDDLLIIISYPRYSRTTLDAARFVKERNTKIVAITDTEE
                     SPAYELADVSLLSKSNIVSFVDSLVVPMAMINQLIINISLREKEEIVEYFNTLERLWD
                     HNSIYQNF"
     gene            complement(1146..1682)
                     /locus_tag="PKH42_02355"
     CDS             complement(1146..1682)
                     /locus_tag="PKH42_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA19300.1"
                     /translation="MKKLEIRLSGSGGQGVILAAIILANAGVEQGLNAIQTQSYGPEA
                     RGGASKAEVILSNGEINYPKISTANILLSLTQKAYDKYKSSLEKDGILIVDESVKLRG
                     DETFKVYSLPILRTALEKFETHMVANIISLGAIYELMGENIIDKDIMIRSITERVPAH
                     TVQKNTDAFEEGIKLARG"
     gene            complement(1693..2508)
                     /locus_tag="PKH42_02360"
     CDS             complement(1693..2508)
                     /locus_tag="PKH42_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOA19301.1"
                     /translation="MNCNEITKKYYRANKLPHIWCPGCGHGTLTNTIVRAIDKLGLNK
                     DEVVIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKFANPNLHVIVIMGDGDSSA
                     IGGNHLIHAARRNIDITAVVFNNNIYGMTGGQFSPTTPSGDMATTTPHGNIDKPFDLC
                     SLAQGAGATYVARGTAYHAVLMQKLIENGIQNKGFSFIEGLSLCPTYYGRKNKKGSAF
                     KMHTFLKENCVDKKVVDKDPEKGKNKILIGEFYNNPQPEYTESYQAVIDKLQG"
     gene            complement(2508..3644)
                     /locus_tag="PKH42_02365"
     CDS             complement(2508..3644)
                     /locus_tag="PKH42_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOA19302.1"
                     /translation="MSNKVYKLVQGNEACVEGALAAGMRFFAGYPITPSTEIAEISAE
                     KLPALGGRFIQMEDELASMAAIIGGSLAGKKSMTATSGPGFSLMQENIGYAAITEIPC
                     VVVNVQRGGPSTGLPTSPAQGDVMQAKWGTHGDHPAIALTPNSVQETYEYTVKAFNLA
                     EKYRTPVILLMDETVGHMREKMAFKDVSEIEIIDRKKPTCKTEDYVAYRPDEDMIPPM
                     ASFGEGYRYHVSGLTHDETGFPTNNNEISEKLVKRLVDKVNKNLDDFSYYEEYKLEDA
                     EIVIIAYGGVSRSAKSAVDELRKQNIKAGLFRPITIWPFLEKQVNKVSLNAKEIYVAE
                     MNLGQYFLEVERAACKNCTVKNISKVNGELITPEEIVQAIGGNK"
     gene            complement(3641..3838)
                     /locus_tag="PKH42_02370"
     CDS             complement(3641..3838)
                     /locus_tag="PKH42_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA19303.1"
                     /translation="MAEEKILLQNIPWCKGCGVCVGFCPVKVLEIKDDKCVIAHRDKC
                     IFCGQCELRCPDNAIWIGGKK"
     gene            complement(4068..4640)
                     /locus_tag="PKH42_02375"
     CDS             complement(4068..4640)
                     /locus_tag="PKH42_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA19304.1"
                     /translation="MDKMYGQITEMVHFLIKGSYEGKEDLRFVDLTCGNGYDTLFLSN
                     LAGKKGFVTAFDIQETAIERTKKLLQEESNFNNYKVIHNGHEHIDKYLTEKIDAAIYN
                     LGYLPNFNKDIFTKPETTICSINSLFPYLKNSGRIYITAYTSHDKGCEINSIYDYLIK
                     LDKKEYNVIHIKVINKNNTPPELFIIEKNS"
     gene            complement(4643..5350)
                     /locus_tag="PKH42_02380"
     CDS             complement(4643..5350)
                     /locus_tag="PKH42_02380"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOA19305.1"
                     /translation="MEERLQKYIARCGVASRRKAEEIILQGNVKVNGIIIKELGSKIN
                     PEKDIVTVNNQRIYEKQKNIYIKMYKPEGYITTVKDQFNRKTVLDLINIKERIYPVGR
                     LDYDTSGLLLLTDDGDLANKLMHPKYRIFKTYEAEIEGGISEDSINKLRNGVLIDGYK
                     TAPAKVKLLKKTQKNSFVQISIHEGKNRQVRKMFEKVGHRVIKLKRLSFGNINLDGLR
                     EGQWKYLTDDEIEYLKR"
     gene            complement(5502..6533)
                     /locus_tag="PKH42_02385"
     CDS             complement(5502..6533)
                     /locus_tag="PKH42_02385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOA19306.1"
                     /translation="MIFKRAIAILTVIFIFTCEFTCVEAMPGVSAQAYIVIDAQSGRI
                     LKSHNENKRLPIASTTKIMTAILAIETIDDLNQRIEVPPECSGIEGSSIYLRPKQMVS
                     VIDLLYGTMLRSGNDAAETLSKFAGRNNPEGFISMMNKKAKELGALNTNFENANGLHD
                     DNHYSTAYDLALISAYAMKNDTFRKIVSTEKYKAESMNNMFYNKNKVVYQYKYGTGIK
                     IGFTKAAGRCIVASAEKDGTEIIAVLLNDNNWFNDSYQIFDWAFENYKCYSIVEKGQF
                     VKYTPERNPVFIGESFRCVLTDKEIKQIRFEALIKPELMSNDSETKTCGTYNIYLGKT
                     KLGTGYLIR"
     gene            complement(6523..6903)
                     /locus_tag="PKH42_02390"
     CDS             complement(6523..6903)
                     /locus_tag="PKH42_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19307.1"
                     /translation="MSKDIESIIKTTLDNINNLTENEKLISSKICYDNTNYLAISKLN
                     MNFIIGGSDIDKKFRTVDLKPFAGAAYVNLQLNPQILIYETPDNNIKTININNETTAG
                     DITSSIMNIMSFIKEIREKKDCDI"
     gene            complement(7049..7420)
                     /locus_tag="PKH42_02395"
     CDS             complement(7049..7420)
                     /locus_tag="PKH42_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19308.1"
                     /translation="MGILIVLLGGLLFLYLNIKCAFKINITFYFLHVYFYVIILRKKH
                     EYQKRINFSAKKIIDRYKAPELRQKTKNNLKYLKHIKKVFNIFYVKDIFFYPECILEK
                     QSFALEFVIVNRMLKKSLLNG"
     gene            complement(7497..8021)
                     /gene="scpB"
                     /locus_tag="PKH42_02400"
     CDS             complement(7497..8021)
                     /gene="scpB"
                     /locus_tag="PKH42_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOA19309.1"
                     /translation="MNIKSVLESLLFAWGEPLSIIEMSKILEKPADSIILVLDEMISE
                     FNDNEDRGLIIQKFGDSYQLTTKKANYEYIQKLLETTVSKSLSTAALETLSIIAYKQP
                     VTKVDIERIRGVKCSQVVKGLIDKGLIKEVGRLDKPGRPVIFATTDEFLRHFGLSSID
                     ELPSIGDIASTVEV"
     gene            complement(8018..8746)
                     /locus_tag="PKH42_02405"
     CDS             complement(8018..8746)
                     /locus_tag="PKH42_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOA19310.1"
                     /translation="MDYSVNINVFQGPFDLLFHLIEKKEIDIYDIPISEITDQYLEYL
                     EEMMQFNMNVASEFIFMAATLVEIKSQMLLPQKEKEEDPRQELVNKLLEYKMYKDLSE
                     ELKKYEDESSFYISKPREEMAITSDTKTEQLSLNEINVYELYNIYLSLIKKQNFRIEP
                     EEKFKIYREKYSVKGCMEDIVKKIKKFGKVSLFNTLKEKENISKEYIVTLFLAILELS
                     NNKGMKIYQENIYGDIIITKQERK"
     gene            complement(8759..9913)
                     /locus_tag="PKH42_02410"
     CDS             complement(8759..9913)
                     /locus_tag="PKH42_02410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOA19311.1"
                     /translation="MKKILTIFFIFIILNMMVVTAYGIEPEDLQSRSAILINADDGRI
                     LFEKNANDKMQPASITKIMLMVLISERMADGQITLDQEMTISENAAGMGGSQIYLEAY
                     EVQTVENMLKAISMRSANDASVAMAEFMYGSEESCVKAMNEKAKELGMKDTVFTNVTG
                     LPDPEHLTTANDIAIMTRELLKYNYMDEYMLTWMDSVFVGKEKDSEQVLVNTNRLINN
                     YNGLLAGKTGYTTEAKYCLSAAARRNDTTLIAVVLGCDNTKIRFDEVTKLLNEGFANY
                     KNIVFHKAGDTITTSPVYCGKEETINIVSKENINYFTESNCKPEDFNLEYVIDENLKA
                     PLNVDKKIGRAILSKDGRVLGEFELYPGANVEKENLFKFFINNFLKTTIR"
     gene            complement(10043..11221)
                     /locus_tag="PKH42_02415"
     CDS             complement(10043..11221)
                     /locus_tag="PKH42_02415"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOA19312.1"
                     /translation="MKRTVLIVLDSVGAGELPDAAAYGDEGSNTLKNIYKNAKNFSLP
                     HLENLGILNIDGFEDLKSPEYFLGSVAKCSQKSKGKDTTTGHWEISGLILDKPFPTYP
                     DGFPEELISKFEKKIGRKVIGNYAASGTEIIKELGKEHVETGNIIVYTSADSVFQIAA
                     HEEVVPLDELYEICIIARKMLQGNNGVGRVIARPFIGEEGNYTRTGNRKDFSLEPYKD
                     TMLDFVKNSGREVYAIGKIEDIFANKGITYSNHTKNNEEGIEALIQTVKLDFEGLIFA
                     NLVDFDMIYGHRNNVQGYGDALMYFDGRLPEIMDNLKDDDILIITADHGCDPTTASTD
                     HSREYIPLIFYGKNIKENNNLGILETFASIGKTVLDIFDIKNDLKGTSVKNQIIKTGK
                     "
     gene            complement(11335..12201)
                     /locus_tag="PKH42_02420"
     CDS             complement(11335..12201)
                     /locus_tag="PKH42_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOA19313.1"
                     /translation="MEITMYKEYLYQRNLSENTIDSYIYDVESFRNYLCEDYELELTE
                     TKKAHILTYLVTLQKNGKSSSTLSRTISALKNFFDFLKNEKLIKDNPAVSIHSPKQIK
                     KKPSILTEEEVAQLMLLPDKNTFKGIRDLAMLELMYSSGIKVSELISIKLSQINLSAG
                     IISIEGEKERIIPLSKYAISAIENYLNNFRDKNTDENDFLFINITGEPLTRQGIWKSL
                     KSYKKKMNLNKELSPQILRNSFAVHMLSHGADLGTIQELMGLNSLGAAQNYINNLEFK
                     SLEVFNKTFPRG"
     gene            complement(12201..12782)
                     /locus_tag="PKH42_02425"
     CDS             complement(12201..12782)
                     /locus_tag="PKH42_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19314.1"
                     /translation="MNNTLKKLVKSENKKFIALILIFIGALILSALIYSLTGKGSLSE
                     INYESISKMNFLQIFGSSIKRNIIYFLAVIFLTYFGQGYLTMILFGFISVYYGLSVIY
                     IIRTVGMDLKYFMLTFTDYFIFFPILLYFTFISSSIAKYTKKAKNIETISRKFDIIIS
                     GYLRISLFYLLIVTAYSFVYSLYVLILSRLMVR"
     gene            complement(12913..13437)
                     /locus_tag="PKH42_02430"
     CDS             complement(12913..13437)
                     /locus_tag="PKH42_02430"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOA19315.1"
                     /translation="MVNKEITVKSEKIFEGKIVNLRVDTVELQNQKYAKREIVEHKGA
                     ACILAVNNDKVIFVRQYRKAIEENILELPAGILNIAEEPPEGALRELKEETGYKAGKI
                     ELIYEFYTSPGFSNEKVYLFKASELVYEGTDLDEDECLETIEVDRDEAKKMLETGKIN
                     DSKTIIGLLHWLNS"
     gene            complement(13548..14342)
                     /gene="spo0A"
                     /locus_tag="PKH42_02435"
     CDS             complement(13548..14342)
                     /gene="spo0A"
                     /locus_tag="PKH42_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOA19316.1"
                     /translation="MQKKVKVVIADDNKEFRLILKDFLLSKKVFDVVDMAEDGIKTLE
                     SVEKHEPDILILDIIMPQLDGLGVLERLHKKELKKFPKVIVLSAVGHDKVTQRAINMG
                     VDYYIVKPFNFESFADRLLQISELETSKVQNKNKEIVYNEKINSELSLEVKITEILHE
                     VGVPAHIKGYQYLRTSIIDVVKNVELLGAITKELYPMIAAKYSTTPSRVERAIRHAIE
                     VAWTRGKIETINNIFGYTIHNNKGKPTNSEFVAMIADKLRLEQRVS"
     gene            complement(14561..15637)
                     /gene="spoIVB"
                     /locus_tag="PKH42_02440"
     CDS             complement(14561..15637)
                     /gene="spoIVB"
                     /locus_tag="PKH42_02440"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HOA19317.1"
                     /translation="MRNFLRRVLFFTFFIAVLMTAVFFGFNLKTNPYKFAKGDQVIDT
                     EGKYIIPGGQTIGIELKTEGVLVVGLADIINIDNISTSPAKIAGIEIGDKILEIDSIY
                     MRSTDEILNYTETMGIKVYTFKIERAGEIYNLTITPVQRYDSDEIKFGFWARDDIAGI
                     GTVTFVDPETGFYSAIGHGISDSETGKLIDIESGTISKANITNIKLGKKGEPGEIIGY
                     ILKDKESLGTVTDNTDFGIYGKINKDSMGYFSGDLMEIGKKEEIKLGPAYIISCVNNE
                     IKKYSVDITKIFYQNKPDEKSFVIKITDKELLEMTNGIIQGMSGSPVIQNNKIVGAVT
                     HVFMNDPTKGYGIYIEWIIDKIYD"
     gene            complement(15688..17346)
                     /gene="recN"
                     /locus_tag="PKH42_02445"
     CDS             complement(15688..17346)
                     /gene="recN"
                     /locus_tag="PKH42_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOA19318.1"
                     /translation="MLLSLNISNFAVFENVSVDFDSGFNVFTGETGSGKSVLINAIEL
                     ILGDRASKELIRKGKNSALIEGIFDIGDNDSLYEDLKSLNIEIDKDDFLIISRELLQN
                     GKSINKVNGRTYPLNTIKAIGSLLIDIYGQFGHESLFKKENHIKMLDSLIQNELSPLL
                     QSYNELFVKYNRVINEISTLKEKLLNKDKKIEQLQYEINEIDSAELKEFEEEELLKNI
                     KKLSNIQEIKKSLYDAIQILNSDNINNVYSIINKIKNYDEKLEEFLSRVDIQLEELKL
                     LSYDLRDYSENLDLDEKKLDNIENRMSLINRLKRKYGFSIEKINEYRNASHEELSLLL
                     KADSKLNSLITEKNEIYNLLIEKAGIISKKRKTAAEYLEKQILIELSELNMKNIEFEV
                     KINKKNISADGVDDVEFLISTNVGSDLNSVQKIVSGGEASRIMLAFKKIKSDIGQTMV
                     FDEIDMGISGKTAQMAGVKMNYIAKNNQVICVTHSPQIASISKNHFLIEKKVVDNNTF
                     STVKKLDKENKIYEIARLLSGMKITEKSINNAKELIEFNSKISE"
     gene            complement(17357..17809)
                     /locus_tag="PKH42_02450"
     CDS             complement(17357..17809)
                     /locus_tag="PKH42_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOA19319.1"
                     /translation="MKKYTRQTKILELISKSEIETQEELAEALKNMGIDVTQATISRD
                     IKELRLVKVMSKSGKYKYATISQSQEGITDRLNKIFENSVVSIDNAMNMIIIKTIPGA
                     AQICASAIDFMGIEEIVGTLAGDDNVFVAVRKIEQVDKVLQEFKKNIR"
     gene            complement(17829..18614)
                     /locus_tag="PKH42_02455"
     CDS             complement(17829..18614)
                     /locus_tag="PKH42_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOA19320.1"
                     /translation="MKNYRLDVFLHNKGYVGSREKAKELILNNSVFVDDKLITKPSYM
                     VNDDVKIKITEDVYISRGYLKIEKAIHVFNINIENKTAVDIGASTGGFTDYLLKHGAK
                     KVYAIDVGCGQLHDSLKNDIRVVNLENTNFRYIDTSIITDIIDIITVDVSFISLQHII
                     PKIVELSHNDTDIVALVKPQFEAGKVNIGKKGIVKKREVHLGVLNSINNYCKTNNLKL
                     INMDYSPIRGGAGNIEYLAHLKITGNTNEINFKDLINKAFEKL"
     gene            complement(18812..19006)
                     /gene="xseB"
                     /locus_tag="PKH42_02460"
     CDS             complement(18812..19006)
                     /gene="xseB"
                     /locus_tag="PKH42_02460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOA19321.1"
                     /translation="MEYDNFESALEELKNIVRKFENKDDISIDELLENYEKGMKAYSF
                     CMKKLDDTQRKIKIIDENIE"
     gene            complement(19008..20189)
                     /gene="xseA"
                     /locus_tag="PKH42_02465"
     CDS             complement(19008..20189)
                     /gene="xseA"
                     /locus_tag="PKH42_02465"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOA19322.1"
                     /translation="MKLKPIKVSVLNQYIKKYLMSNSILNNLRVEGEITNIRISKTGY
                     TYFSLCDEISCISCVAFFADTISKNGDKIIAEGELSVYEAKGTYQLIVRDIERAGLGK
                     ILLDLELLKEKLKEQGLFDRKRELPEFPQRIGIITSKTGAAIKDILKTFENVKANIDI
                     TIYNTLVQGEKSKKSIIEGLKYFNSVETDVILISRGGGSFEDLNVFNDMDVAMEVYKS
                     NIVVVTGIGHETDRTLTDYVADVYCHTPTAAAERIIRNYKDIEEKLDNLVFTLKSETN
                     NTISLKKAELISNKYLLRSSLPLEDIYKTKNQLDTYKNLIKNKTTSQINEYYFNLKIM
                     LEKLNTFDYKKQLEKGFALVSDNSGNIIKHKEQVELKDILHIRFADFKIEAETRDIKE
                     V"
     gene            complement(20170..21117)
                     /locus_tag="PKH42_02470"
     CDS             complement(20170..21117)
                     /locus_tag="PKH42_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19323.1"
                     /translation="MRYILGIDTSSYTTSVALINEESNEILADERKVLEVKKGQKGLR
                     QQEAVFQHLKNLPELYEKIPYDLSNIKIVSVSSKPRNAENSYMPVFIAGRNFGKVISD
                     TLNCEFLEYSHQENHIAASIINNYKNIDEFLAVHISGGTTEFLSCNKAIKGFDIKIVG
                     GTKDITFGQLIDRLGIMMGFIFPCGKYMEEYIKDKTFEKITRPRLIKGSYINLSGMEN
                     YFGNLLNKYSKEVIITSLFDYIAEYIVHIISSLSHLQHKTLIISGGVASNSYIRNYIT
                     KSVKGYEVLFPAVNNSSDNALGLAYLPIIDRWHNEIKTN"
     gene            complement(21114..21518)
                     /gene="nusB"
                     /locus_tag="PKH42_02475"
     CDS             complement(21114..21518)
                     /gene="nusB"
                     /locus_tag="PKH42_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOA19324.1"
                     /translation="MKRKETREEAVKIAYSMDVNKDFNKNNITEYLEHFELENMDIDY
                     LNKTISDMIDNMEEIDKHITDNSKDWKLSRIAKVDLAVLRIALSEILYNETIPESVSI
                     NEAVEISKKFSKEDSHKFINGILGTVVRCIKK"
     gene            complement(21617..22021)
                     /locus_tag="PKH42_02480"
     CDS             complement(21617..22021)
                     /locus_tag="PKH42_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOA19325.1"
                     /translation="MDNEKFEFGQVKISDDVVIIIAGIAASQVKGVNTTRTGVTDGIT
                     NLFSKNNYSKGIKVDVNEDTVVVDIFINVEYGYKINEVAREVQLAIKKEIETMTDLKV
                     AAVNVHVLNIVQEKIQEKVQEKEKDAANIAIE"
     gene            complement(22064..22711)
                     /locus_tag="PKH42_02485"
     CDS             complement(22064..22711)
                     /locus_tag="PKH42_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HOA19326.1"
                     /translation="MIMRKETLVFLASLAIIFIIGYINMSLTPDNAFDDLETGQMEPA
                     DLEGDIAEIIEGGVNEVDFAEVLPEETDYEILGDITDLSSAEEASSEGVLGVLNASFA
                     NFKLNKEKSNMDVLDYLEESLSSSLISDETKEQFEQLLLKKNSFIEAEQGIELMLQSK
                     GYNESVAIVDESMVKIVTNDTIEKADATVILDVVVSETNYTPSQIKIVKFDNIDL"
     gene            complement(22721..23263)
                     /locus_tag="PKH42_02490"
     CDS             complement(22721..23263)
                     /locus_tag="PKH42_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19327.1"
                     /translation="MNEFFKMLKENKKLSYAVNVFILLVIISLILKLDFSKVSGEPVS
                     YQDSEVKTESYVYALEKRMEEILSKIDDINSVDVMIYTKQTPILEPVYDENISNETNI
                     ESMSDGTKREVNRETKQNKVILGRDNSVVERYYQYPEISGVLVVVNYSGNKDIYSILI
                     NSVKTLLDIKLNDIEIIVIN"
     gene            complement(23256..23543)
                     /locus_tag="PKH42_02495"
     CDS             complement(23256..23543)
                     /locus_tag="PKH42_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19328.1"
                     /translation="MNVKLPVTTEVPAFQREVNVKSISPEIIESINKENFQGEVVSNI
                     ILRFQEDMQISAIVNLRKMIGKDELDRLKSNIAEIFQLSSDKIEINGGAYE"
     gene            complement(23702..24835)
                     /locus_tag="PKH42_02500"
     CDS             complement(23702..24835)
                     /locus_tag="PKH42_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19329.1"
                     /translation="MKKITLFIIVFTVLFTFTCKASTMDEAMDEIPTKGIEDYINEKN
                     AYFRENNIRIRDLIRNAFTGNLDVSLKDYLYYELKKEQSFFKDILKTGINVFIICIAL
                     TIINYFTDEDTGKSVSDIVVLFSVIIIFTIILKDVLSIKSILKSDFTDFKTITEEINA
                     IFMAAMLTFGKLSLLQFFQTSLNYTIGITTQFIYSFIDIMTVIMIAVILTNNMNELIN
                     AKLLYKALKKGTLLILTGFMIIVVINFSVQGYILYKTDNIFISSLKAMSPASLPVVGN
                     AVTSFFGVFLKSLLMIKDVLGIVVIVFILSSFGGSIIKISIALIIYKVLGVVTEPFNE
                     NISNLIYEMADIFYIYLICLITPLVIVTVYYSIIINYMNNILG"
     gene            complement(24840..25229)
                     /locus_tag="PKH42_02505"
     CDS             complement(24840..25229)
                     /locus_tag="PKH42_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HOA19330.1"
                     /translation="MLLMKIILIAVITLIMGITLSKFNSEFKVYITVIFGIVVIFLLF
                     KELRVYLEEIVDLFVKYDIKTEYFGTILKIVGIAYICDFISLLCKDLDYESVGKKVEI
                     AGKLIILIYSIDVIKIFLDQILILVNG"
     gene            complement(25241..25438)
                     /gene="spoIIIAC"
                     /locus_tag="PKH42_02510"
     CDS             complement(25241..25438)
                     /gene="spoIIIAC"
                     /locus_tag="PKH42_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOA19331.1"
                     /translation="MDIDLIFKIGAIGIVVAVFNQILTKTGRDEQAMFVTLAGVIVVM
                     ALIVGMMNELFTEVKSVFNLY"
     gene            complement(25448..25951)
                     /locus_tag="PKH42_02515"
     CDS             complement(25448..25951)
                     /locus_tag="PKH42_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19332.1"
                     /translation="MIWIRIALFAGTVVTVKYIFSSINHDGDEKIKKMKELINFTEYL
                     RVYSCDMKMSIEEVCLKYNFKSIETEVIIKSMINILKNKKSSKELLVNINKIMSTPEA
                     FNAYFTEIIDYYGCTYSEVLDRKLKFTIEEMERAMKEFSIKHNEKKTLNNRISFLAGC
                     LAAIIMS"
     gene            complement(25938..26861)
                     /gene="spoIIIAA"
                     /locus_tag="PKH42_02520"
     CDS             complement(25938..26861)
                     /gene="spoIIIAA"
                     /locus_tag="PKH42_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOA19333.1"
                     /translation="MLNLLNHLETRIKNIIIRDCGILDEIIEIRLRINSPVLIKTLKR
                     EFFLDESIVITKKDIDDILGNLTKNSIHAFENEINKGYITTEGGHRVGISGDCIYDKN
                     GLKGFKNITSLNIRIAKEFPGCSEKALKHFITPDKNIYNTLIIGPPLSGKTTLIRDVA
                     RNLSDGFKNPYFEGCDVTLIDERGEISAVYNGIPQMNTGRRTDVLSYCKKSDGFFMSI
                     RALSPKVIVSDELGSLEDFEIIQYALKSGIKVVATAHGFSLDDVKRNLYLKNIIDNNF
                     FERAFVLDKRPLKVNEVFDFQKNRLIYNDLD"
     regulatory      27131..27244
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27349..27960
                     /locus_tag="PKH42_02525"
     CDS             27349..27960
                     /locus_tag="PKH42_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA19334.1"
                     /translation="MKSDVTMNQRINTKTITKVGILGAVATVLMLLEFPLWFAPSFYE
                     LDFSEVPVLLGAFALGPAAGAMIELVKILINFVLNGTDTGGVGEFANFIIGCAFIVPA
                     GFIYKRNKSFKTAILGLVVGTLTLALAGAFLNYYLLLPLYAKIYGYPIQAFIDMGNAL
                     NPAITDLKTFVLYAVVPFNLLKGTIVSAMVILIYKKLSPILHR"
BASE COUNT         8285 a         5201 c         3853 g        10774 t
ORIGIN      
        1 atttcaatga ttattagacc tttattttct tacaagtgtc aaagacagga ttatagtaca
       61 tattattaaa tataacaaga aaattttttg gcacagttaa acaaaaggat acaaagtata
      121 gggtatttcc ttaacgctca ataaacatgc tcttgttata agcgtctccc ctttctttat
      181 atccttaatg attattctta ctttagagcg tcgctctttt aatttaagga cattcctctg
      241 atccagagac tttcccaaaa cagaggtgta tcccattacc tcacatatat tagaagttct
      301 gatatatact gttatggtcc cagagtcttt ctaatgtatt aaagtattca actatttctt
      361 ctttttccct caagcttata tttattatca attggtttat cattgccata ggtactacta
      421 aggagtcaac aaatgataca atgttgcttt ttgataagag tgatacatct gccaattcat
      481 aggcaggtga ttcttcggta tctgtaattg caactatttt tgtatttcgc tccttgacaa
      541 atcttgcagc atcaagagtt gttctggaat atcttgggta actaataata atcaataaat
      601 catcttcttt aattcttata atttcttcat ataaggaatt aacaccgctg ttgttgagta
      661 ctttagcgtt attcaagatt acatccaaat agaagcctag ataatttgac aaagttgaag
      721 atgttctaag ccctagtaca aatactctgt cagcctctaa gattagctcg gttgctttat
      781 ctaatgcttc catatcgaaa tggtcataca aataacgcaa gttgttcatc tcattcttta
      841 aatttctttt atgaagcttt aacttgtcat ttacatcatc attaagactt attctctgga
      901 ccgtggtaag tttattttta attaaaacct gtaaagcttt ttgcatttca ggatatccgg
      961 aataacccaa ggccgatgca aatcttacaa cggttgattc gcttacatct actgtttctg
     1021 ctatttttgc agcagtcata aatgcagctt tgtcatagtg gtcaataata aattgagcta
     1081 ttagcttttg tcctctgctg aatttatgaa agtttttttt gataatgctt attaaatcat
     1141 tcatgttacc ctcttgctaa tttaatacct tcttcaaatg catccgtgtt tttttgaact
     1201 gtatgagccg gaactctttc agttatggag cgtatcatta tatccttatc aattatgttt
     1261 tctcccatca attcataaat tgcacctaat gaaattatat tggctaccat atgagtttca
     1321 aatttctcta gtgcagttct caatatggga agactatata ctttaaatgt ttcgtctccc
     1381 cttagtttaa cactttcatc aacaattaat ataccatctt tttccagaga tgatttgtat
     1441 ttatcatatg ctttttgagt aagagaaaga agtatatttg ccgttgatat tttaggataa
     1501 tttatttcac cattgctcaa tataacttct gccttactgg caccgcccct tgcttcaggt
     1561 ccgtaggact gagtttgtat agcatttaat ccttgttcga ctccggcatt cgctaatata
     1621 atggctgcta atataactcc ctgacctccg gaaccgctaa gtcttatctc taattttttc
     1681 atttttctcc ccctatcctt gaagtttatc aataacagct tgataacttt ctgtatattc
     1741 aggctgcgga ttgttgtaaa attcgccgat taatatttta ttcttgcctt tttcaggatc
     1801 tttatcaact acctttttat caacacaatt ttcctttaaa aaagtatgca ttttaaaagc
     1861 actgcctttt ttgttttttc ttccgtaata tgttggacac aggcttaaac cttctataaa
     1921 cgaaaatcct ttgttctgta ttccgttttc tattaatttc tgcataagaa cagcatggta
     1981 agctgtacct ctggcgacat aagtagctcc tgctccttga gccaagctgc aaaggtcaaa
     2041 aggtttatca atattgccgt gtggtgtagt tgttgccata tcaccggaag gagtagtagg
     2101 tgaaaattgt ccgcctgtca ttccgtaaat attattgttg aatactactg ctgttatatc
     2161 tatatttctt cttgcggcat ggattaggtg attgcctcca attgcagaac tgtctccgtc
     2221 tcccattatt acaataacgt gaagatttgg atttgcaaat ttaatacccg ttgcaaaagc
     2281 caatgccctt ccatgagttg tgtgaagagt attaaaatcc aaataaccgg gagctcttga
     2341 tgaacatccg atacctgata ctattactac ctcatcttta ttaagtccta atttatctat
     2401 tgctcttact attgtatttg ttaatgtgcc atgaccgcat cccggacacc atatatgagg
     2461 gagcttgtta gctctgtaat attttttagt tatttcgtta cagttcatta tttgttacct
     2521 cctattgctt gtacaatttc ttcaggagtg attagttcac catttacttt acttatattt
     2581 ttaacagtac aatttttgca tgcagccctt tcaacttcaa gaaaatattg tcccaagttc
     2641 atttctgcaa catatatttc ttttgcgttt aatgaaacct tatttacttg tttttctaag
     2701 aaaggccaaa tggtgattgg tctgaataaa cctgctttaa tattttgttt ccttagctcg
     2761 tctacagcac tcttagctga ccttgatact ccgccgtatg cgattataac aatttctgca
     2821 tcttctaatt tatattcttc ataatatgaa aagtcatcta aatttttatt aactttatca
     2881 actaatcgtt taactaattt ttcggatatt tcattattat ttgtaggaaa accggtttcg
     2941 tcatgagtaa gtcccgatac atggtatctg tatccttctc cgaatgatgc cattggtggt
     3001 atcatatctt catcaggtct ataagcaaca tagtcctcag tcttgcatgt tggttttttt
     3061 ctgtctatta tttcgatttc cgaaacatct ttaaaagcca ttttttccct catatgtcct
     3121 actgtttcat ccattaggag tatgaccgga gttctgtatt tttcagctaa attaaaagct
     3181 ttaacagtat attcatatgt ttcttgaaca gaattaggtg tcaaggctat agcaggatgg
     3241 tctccgtgtg ttccccactt tgcctgcata acatcacctt gagcaggaga tgtcggaagt
     3301 cctgtactcg gtccacctct ttgcacattt acaacaacac aaggtatttc ggtaatagct
     3361 gcatacccta tgttttcctg cattaaagaa aacccgggac cgctggttgc agtcatagat
     3421 tttttccctg cgagagagcc tcctattatg gcagccatac ttgcaagctc atcttccatc
     3481 tgaataaatc tgcctcccag cgctggcagc ttttctgcag atatttccgc tatttcggtt
     3541 gatggcgtta tgggataacc ggcaaaaaat ctcataccgg cagctaatgc accttcaacg
     3601 caagcttcat ttccttgaac taatttgtat accttattac tcatttttta cctccaatcc
     3661 aaatagcatt gtccggacat cttagttcac attgtccaca aaaaatacat ttatccctgt
     3721 gagctatgac acatttatcg tctttgattt ccagtacttt tacgggacaa aaaccaacgc
     3781 atacaccaca tcctttgcac catggtatat tttgtaaaag aattttttcc tcagccatat
     3841 aattcctcca tataatttcg ttatcttaat tataacagat tcgaggtttt cttgcaagaa
     3901 atatatcaaa taatattgca taatgcaagt tttatttcat tatctgcatt taattggaag
     3961 gaaaacgttg ctttttaact aaatcgcaca tataaatgaa tattttgcaa gaaatgagca
     4021 agcagtatca tatgacaagg ggacgggggt attgtcatta attagtatta tgaatttttt
     4081 tctataataa ataattcagg aggagtgtta tttttattta taacttttat atgaattaca
     4141 ttgtattctt tcttgtctaa cttaattaag tagtcgtaaa ttgaattaat ttcacagcct
     4201 ttgtcgtgac ttgtataagc tgtaatatat atccttcctg agttttttaa ataaggaaaa
     4261 agtgaattta tagagcagat agtagtttcc ggttttgtaa aaatatcttt gttaaaatta
     4321 ggaagatatc ctaagttata tatggccgca tctatttttt ccgtcaaata tttgtctata
     4381 tgctcatgtc cattatgaat aactttataa ttattgaagt tactttcttc ttgaagtagt
     4441 ttttttgttc tctcaattgc tgtttcttgt atgtcaaagg ctgttacaaa accttttttc
     4501 cctgctaaat tgcttaaaaa tagagtgtca tatccgtttc cgcacgttaa atcaacaaat
     4561 cttaaatcct cttttccttc atagcttccc ttgattagaa agtgcaccat ttccgtaatt
     4621 tgaccgtaca tcttgtccat ctttacctct ttaaatattc tatttcatca tcggttaaat
     4681 atttccattg tccttctctc agtccgtcta aattgatatt gccgaatgat agtcttttca
     4741 attttatcac cctgtggcct accttctcaa acatttttct tacctgcctg tttttaccct
     4801 catgaatgct tatttgtacg aacgaattct tttgagtttt ctttaacaat tttaccttgg
     4861 caggagcagt cttgtatccg tcaattaaaa cgccgtttct taatttattt atggaatctt
     4921 cgcttattcc tccttcaatt tccgcttcat aggttttgaa gatgcgatat ttgggatgca
     4981 ttaatttgtt ggcaaggtct ccatcatcag ttaataacag taatccggaa gtgtcatagt
     5041 caagtcttcc tacgggataa atcctttctt taatattgat taagtcaaga acagtttttc
     5101 gattaaactg gtccttgacg gttgtaatat acccctcagg tttgtacatt tttatataaa
     5161 tatttttttg tttttcatat atcctttgat tatttacggt tacaatatcc ttttcaggat
     5221 taattttgct tccaagctct tttataatta ttccgttaac ttttacatta ccttgtaaaa
     5281 ttatttcctc agctttacgt ctcgaagcaa ctccgcatcg tgcaatatat ttttgcagtc
     5341 tttcttccat atctcctccg gataacaagg gatgggctct tttccctgtt ttctagatat
     5401 tatattataa atgtatgaat ttgtaaaata ttcaatatgc gttttcagga agcaaccctg
     5461 gtgggcgtga acaaaaacat gacaaagccc ccatcccctt gtcatctaat cagatatcct
     5521 gtgccaagct tggtttttcc caaatatatg ttgtaggttc cgcatgtttt agtttcggaa
     5581 tcatttgaca taagctccgg tttaattaat gcttcaaaac gaatctgttt tatttcttta
     5641 tccgtcaata cgcatctaaa gctctctccg ataaagacgg ggtttctttc gggagtatat
     5701 tttacaaact gccctttttc gactatgctg tagcatttgt aattttcaaa agcccaatcg
     5761 aaaatttgat aagagtcatt aaaccaatta ttgtcattga gaagcactgc tataatttca
     5821 gtaccgtcct tttctgcaga tgcaactata catcggcctg ctgcctttgt aaagccgatt
     5881 ttaattcccg taccgtattt atactggtaa acaactttat ttttattgta gaacatattg
     5941 ttcatacttt cagccttgta cttttcggta ctcacaattt ttctgaatgt atcatttttc
     6001 atggcatagg cacttataag agccaagtcg tatgctgttg aataatgatt atcgtcatga
     6061 aggccgtttg cattttcaaa atttgtattt agagcgccta actcttttgc ttttttattc
     6121 atcattgata tgaatccttc cggattgttt cttccggcaa atttagataa cgtttctgcc
     6181 gcatcgtttc ccgagcgaag cattgttccg tataacaaat caattaccga caccatttgc
     6241 ttaggtctta aatagatgct tgaaccttct atgcctgagc attccggagg cacttctatt
     6301 ctttggttta aatcatcgat ggtttcaatt gcgagaattg ctgtcattat tttggttgtg
     6361 cttgcaatag gcagtctttt attttcatta tgggatttaa gaattcttcc tgattgagca
     6421 tctataacaa tataagcttg tgccgaaaca ccgggcatag cttcaacaca tgtaaattcg
     6481 catgtaaaaa taaatataac tgtcaatata gcaattgctc tcttaaatat cacaatcctt
     6541 tttctccctt atttctttaa taaatgacat aatattcatg atggaggatg taatatctcc
     6601 tgcagttgtt tcattgttaa tattaatagt tttgatatta ttatccggtg tttcataaat
     6661 taaaatttgc gggtttaatt gcagattaac ataagctgct ccggcgaagg gctttaaatc
     6721 aactgtacga aattttttat ctatatcgct tcctcctata ataaagttca tattaagctt
     6781 ggatatcgcc aaataattgg tattgtcata acaaattttg ctgcttatga gcttttcgtt
     6841 ttctgttaaa ttattaatat tatctaatgt cgttttgatt atactttcta tatccttact
     6901 catgttatcc cctttctttt ggaaatcaat aacgaggcaa aatcacaatg gtgagtatat
     6961 tctctgcctc gtttaacagt gttgaaatcg cgtggcaatt tcttccgtta ttttaattat
     7021 gccctaaaaa aaaagaatta taacataatc atccgttaag aagagatttt ttaagcattc
     7081 tgtttactat tacaaattca agagcaaatg attgtttttc caaaatacat tcgggataga
     7141 aaaaaatatc ttttacataa aaaatattaa atactttttt tatatgctta agatatttta
     7201 gattgttttt agtcttttgt cttaattccg gagctttata tctgtcaatt attttttttg
     7261 cggaaaaatt gattcttttt tgatattcat gtttttttct aagaataatt acgtaaaaat
     7321 aaacatgcaa aaaataaaaa gtaatattta ttttaaatgc acatttgata ttcaaatata
     7381 aaaataagag tccccccaat agaactatca atattcccat aataaaaata accctttaac
     7441 agacaaatct gtttctttgt tttgaataaa aagatgacag tacccccgtc cccttgtcat
     7501 acttctacag ttgaagctat atccccgata ctcggcaatt catcaattga gcttaagccg
     7561 aaatgtctta agaattcatc tgttgtagca aaaattacgg gtctgccggg cttatcaaga
     7621 cgtcccactt cttttatcaa tcctttatca ataagccctt ttaccacctg ggagcactta
     7681 acccctctta ttctttctat atccaccttt gtaacaggct gcttgtatgc aattatggac
     7741 agtgtttcca atgcagctgt tgacaaactc ttagataccg tagtttccaa tagtttctga
     7801 atatattcat aattagcctt tttggtagtg agctgataag aatccccaaa cttttgaata
     7861 ataagacccc tgtcttcatt gtcgttgaat tcgcttatca tttcatccaa tactaatatg
     7921 atgctgtcag caggcttttc taatattttt gacatttcaa ttatgcttaa gggctccccc
     7981 caagcgaaca gcaaactttc caaaacactc tttatattca ttttctctcc tgtttggtga
     8041 ttattatatc tccgtaaata ttctcctgat atattttcat tcctttattg tttgaaagtt
     8101 ccaatatggc taaaaacaat gttacaatat attcttttga tatattttct ttttctttta
     8161 aggtattgaa taatgaaact tttccaaatt tcttaatttt ttttacaata tcctccatac
     8221 agccctttac actgtatttt tccctgtata ttttgaattt ttcttcaggt tcaattctaa
     8281 aattctgttt tttaatcaaa gataaatata tattgtaaag ttcatataca tttatttcat
     8341 tcagtgaaag ctgctctgtc tttgtatctg aagttatagc catctcctct cttggtttgg
     8401 aaatataaaa actgctctcg tcctcatatt ttttcagttc ttccgataaa tctttataca
     8461 ttttgtattc gagtaattta ttaactaatt cctgcctagg atcttcttct ttttccttct
     8521 gaggtaaaag catttgagat tttatttcaa ctaaggttgc tgccataaat ataaattcgc
     8581 ttgcaacatt catattgaat tgcatcattt cttcgaggta ctctaagtat tgatctgtta
     8641 tttcagatat gggtatatca taaatatcaa tttccttttt ttcgattaga tgaaacaaga
     8701 gatcaaaagg tccctgaaag acgtttatat ttacggagta atccatttcc agccttcctt
     8761 atcttatggt agtttttaaa aagttattga taaaaaattt aaataaattt tctttttcaa
     8821 catttgctcc cggatacaat tcaaattcgc ctaatacccg accatcctta gatagtatgg
     8881 ctcttccgat ttttttatcc acgtttaaag gagcttttag attttcatct attacatatt
     8941 ctaaattaaa atcctcaggc ttgcaattac tttctgtaaa atagttgata ttttcctttg
     9001 aaactatatt aatcgtttct tcttttccgc aataaaccgg tgatgtggtt attgtgtcac
     9061 ctgctttatg gaaaacaatg tttttgtagt ttgcaaatcc ttcattcaat agttttgtaa
     9121 cttcatcgaa acgaatttta gtgttatcac acccgaggac tacggcaatt aatgtagtgt
     9181 catttcttct tgcagctgcc gataaacaat attttgcttc agttgtataa cctgtcttcc
     9241 ctgccaataa gccgttataa ttatttatca gtctgtttgt gtttaccaac acttgctctg
     9301 aatccttttc cttgcctaca aatacggaat ccatccaggt aagcatatat tcatccatat
     9361 aattatattt taacagctca cgcgtcatta tagcaatatc atttgcagta gtaagatgct
     9421 cggggtccgg aaggcccgta acatttgtaa atacggtatc cttcataccc aattccttgg
     9481 ctttttcatt catagcctta acacagcttt cttctgagcc gtacataaat tcagccattg
     9541 caactgatgc atcgttggct gagcgcatgg atatagcttt aagcatattc tctactgttt
     9601 gaacttcata tgcttcgaga tatatttgac ttcctcccat acctgcagca ttttccgaaa
     9661 ttgtcatctc ctggtctaat gttatttgac catcagccat tctttctgaa atcaaaacca
     9721 taagcattat tttagtaatg cttgccggct gcattttatc gttagcgttt ttttcaaaca
     9781 aaatccttcc gtcatctgca ttaatcaaaa ttgcagagcg ggattgcaag tcctcgggtt
     9841 caattccata tgcggtaaca accatcatat ttaagataat aaatataaaa aatatggtta
     9901 aaattttctt cataaaatta cctccttttt atgtagaata accatatttt gttgaatcat
     9961 acatttaatt ggatgagcaa attcctatct gcggataatg aaatatctaa gttagttgtt
    10021 taaaaaaaat gtctaattta gtctatttcc cggttttgat tatttggttt ttaacacttg
    10081 tccctttaag gtcattttta atatcaaaaa tatccaatac ggtcttgcct atggatgcaa
    10141 aagtttcaag tatgccaaga ttgttatttt cttttatatt ctttccatag aaaatcaggg
    10201 gtatatattc tcttgaatgg tctgtactgg cagttgtagg gtcgcagcca tggtcagccg
    10261 taataatcaa aatatcgtca tccttgagat tatccattat ttccggtagc ctgccgtcaa
    10321 aatacataag ggcatcccca tatccctgta cattgttcct gtgaccgtaa atcatgtcga
    10381 aatctacaag atttgcaaat atcaagcctt cgaaatctag ttttacagtt tggattaatg
    10441 cttcaattcc ttcttcattg tttttggtat gatttgaata tgtaatacct ttgttagcaa
    10501 atatatcttc tattttccca atggcataaa cttctctgcc gctgtttttt acgaagtcca
    10561 gcattgtatc tttgtaaggc tctaaggaga aatctttcct attgccggtt cttgtataat
    10621 taccttcttc ccctataaaa ggtcttgcaa ttactcttcc tactccatta tttccttgca
    10681 acatttttct ggcaattatg caaatttcat agagctcgtc caagggaacc acttcttcat
    10741 gggctgctat ttgaaatacg ctgtctgcag atgtgtagac tattatattg ccggtttcca
    10801 catgctcttt tcctaattct ttgattattt cggttcctga agcagcatag ttgcctataa
    10861 cttttctccc gatttttttc tcaaattttg aaataagctc ttcaggaaac ccgtccggat
    10921 aggtagggaa tggtttatcc agtatcagcc cgctgatttc ccagtgacct gttgttgtat
    10981 ccttcccctt tgatttttgg ctgcattttg ccacagagcc taaaaagtat tccgggcttt
    11041 tcaagtcttc aaatccgtca atatttaaaa tacctaaatt ctccagatgt ggtaatgaaa
    11101 aattctttgc attcttgtat atatttttta gtgtattgct gccttcatcg ccataggcgg
    11161 cagcatccgg aagttcacct gctcccacgc tgtctaagac tatcaatact gttcttttaa
    11221 tcattggttg ctcctttatt tatattaaaa gaagagataa tttatatctc tcttcctttt
    11281 gtcttattat accattaata gtgcagtctg aaacatcaat tatgtatgaa aatatcaacc
    11341 gcgtggaaat gttttattaa aaacttccaa ggatttgaat tctaaattat ttatataatt
    11401 ttgtgcggca cccagactat taagtcccat taactcctgt atagttccga ggtcagcccc
    11461 gtgagacaac atatgtacag cgaatgaatt tcttagaatt tgaggagata attccttatt
    11521 taagttcatt tttttcttat aagattttag agatttccat attccctgcc ttgttaaggg
    11581 ctcaccggta atattgataa ataaaaagtc attttcatca gtgtttttat ccctaaaatt
    11641 atttaaatag ttttcaattg cgcttattgc atacttgctt aatggaatta ttctttcttt
    11701 ttctccttca atagaaatta ttccggcgct taggtttatt tgagataatt ttatgctgat
    11761 gagctcggaa acctttatac ctgatgaata catcagttcc aacattgcca agtctctgat
    11821 tcctttgaaa gtatttttat caggcaaaag cataagctga gctacttctt cttcagttaa
    11881 aattgaaggt ttctttttta tttgttttgg gctgtgtatg ctgacagcag ggttgtcctt
    11941 aatcagtttt tcattcttca agaaatcaaa aaagtttttt aatgcggaaa tagttctcga
    12001 taatgttgat gaactcttgc cgtttttttg cagagttaca agatatgtca aaatatgcgc
    12061 tttttttgtt tcagttagct caagctcata atcttcgcaa agataatttc taaagctttc
    12121 cacatcgtaa atataggagt cgattgtatt ttcacttaag tttctctgat acaaatactc
    12181 tttatacatt gtgatttcca ttatctcacc atcaatctgc ttaatattaa tacatataag
    12241 ctgtatacaa acgaataagc tgttacaatc agtaaataaa ataaagaaat tctaagatac
    12301 ccggatataa ttatatcaaa ttttcgggaa atagtctcga tattttttgc tttttttgta
    12361 tattttgcta ttgaacttga aataaaagta aaataaagta gaataggaaa gaatataaaa
    12421 taatcagtaa aggtgagcat aaaatatttt aaatccatac caacagtcct gattatataa
    12481 ataacggaca aaccataata tacggaaata aaaccaaaca aaatcattgt taaatatcct
    12541 tgtccaaaat atgttaaaaa gatgaccgcc aaaaaataaa taatatttct ttttattgaa
    12601 ctcccgaaaa tttgcagaaa attcatttta cttatacttt catagtttat ctcacttaga
    12661 gacccctttc ccgttaatga ataaattaat gccgatagaa tcaaagctcc gataaaaatt
    12721 aatattaagg ctataaactt tttgttttca gatttcacca gcttctttag tgtattgttc
    12781 atataaacct cccacctact gtctgtatgc caagcaggac ataagacgtc ctttttgcac
    12841 aataaaacct cttgttaagc tttgtataat atatgcttgt acaagaggtt taatactttt
    12901 tatgttttta ttttaagaat ttagccaatg gagaagtcct attattgttt tactatcgtt
    12961 tattttaccg gtttcaagca ttttttttgc ttcgtctcta tcaacttcaa ttgtttcaag
    13021 gcattcatct tcatctaaat cagtaccttc atatacgagc tccgaagcct taaacaaata
    13081 taccttttca ttggaaaaac ccggtgaggt ataaaattca taaattagct ctattttacc
    13141 tgccttataa ccggtttctt cttttaattc acgcagagct ccttccggag gctcttctgc
    13201 aatattaaga atccctgccg gcagctccaa tatattctcc tcaatggcct ttctgtattg
    13261 tctcacaaaa attactttat cattattaac agctaatata caggctgcac ccttatgctc
    13321 aacaatttct ctttttgcat atttttggtt ttgcaattca acagtatcta ctcttaaatt
    13381 aactattttg ccttcaaata ttttttcgct ttttacagta atttccttat tcactatagc
    13441 ctcctatatt aaatcattgc atctgaagca agattaatag ttggacttat tctaataaca
    13501 atacagattt ttgtcaagtt aatttacagg tgcaccattt tctattgcta tgatactctt
    13561 tgctccaggc gaagtttatc tgcaatcatt gctacaaatt ccgaattggt aggtttaccc
    13621 ttattattat gtatagtata accgaaaata ttgttgatag tttcaatttt cccccgagtc
    13681 caagcaactt ctattgcatg tcgtatggct ctttcaactc tgcttggcgt ggttgaatat
    13741 ttagctgcta tcatgggata aagttccttt gtaattgctc ccaataattc aacattcttt
    13801 actacatcta ttatagaagt gcgtaaatac tgataacctt taatatgtgc aggtacacct
    13861 acctcatgta aaatttcggt tattttgact tccaagctga gctccgagtt aattttttcg
    13921 ttataaacga tttccttatt cttattttgc accttagaag tttctaattc tgatatttga
    13981 agtaatctgt cagcaaaaga ttcaaaattg aaaggcttaa caatataata gtcaacaccc
    14041 atattaatag ctctctgggt aaccttatca tgacctacgg cagatagaac tattacttta
    14101 ggaaactttt ttaattcttt tttatgaagt ctctcaagta cccccaaccc atcaagctga
    14161 ggcattatta tgtccaatat aagaatgtcg ggttcatgtt tttcgacaga ttccaatgtt
    14221 tttattccat cctctgccat gtctactaca tcgaagactt ttttgctgag caagaagtct
    14281 tttagaatta acctaaattc tttattgtcg tcagcaataa caacttttac ttttttttgc
    14341 ataagtactc ccccattcaa aatattctat actaatataa tacgcaaaag aatcaaaaaa
    14401 cccttttttt ttataaaaaa aattttttgt cttctaattt tgtaatataa aaagtatata
    14461 tgtctattat atacagcttt tcctaattta tactaaaatt ttgtcgataa attaaaatat
    14521 ttgtttttaa atttttgtta tattttacta atgtatttta tcagtcatat attttgtcaa
    14581 ttatccattc aatatatata ccatatcctt tggtaggatc attcataaaa acatgtgtta
    14641 cagcaccgac tattttattg ttttgtatta cagggcttcc gctcatacct tgaataatac
    14701 cattggtcat ttccaaaagt tccttatctg taattttaat aacaaagctt ttctcatcag
    14761 gtttattttg ataaaatatt ttagttatgt ctactgagta tttttttatt tcattattta
    14821 cacatgaaat tatatatgca ggaccgagtt taatttcttc cttttttccg atttccatta
    14881 aatcgccgct gaaatatccc atactgtcct tatttatttt cccgtaaatt ccaaaatcag
    14941 tattatcggt caccgttcca agactttctt tatcttttaa aatgtagcca ataatttctc
    15001 ccggttctcc ctttttgcct aacttaatat tagtaatatt tgcttttgat atagtgcctg
    15061 attcaatatc tatcaactta cctgtttccg aatcagaaat accgtgacct attgcactat
    15121 agaatcctgt ttccggatct acaaatgtaa ctgttcctat tcctgctata tcatctcttg
    15181 cccaaaatcc gaatttaatt tcatcgctgt catatctttg gacaggagtt atagtcaaat
    15241 tgtaaatctc cccggctctt tcaattttaa atgtatagac tttaataccc atagtttcgg
    15301 tataatttaa tatttcatcg gtgcttctca tatatataga gtctatttcc aatattttat
    15361 ctccaatttc aatcccggca atttttgccg gagatgttga tatattgtca atattgatta
    15421 tatcagcgag tccgacaacc aatactccct cggttttcag ctctattccg atagtctgac
    15481 caccgggtat aatgtacttt ccttccgtat ctattacttg gtcgcctttt gcaaatttat
    15541 atggatttgt ttttaaatta aaaccaaaaa aaaccgcagt cattaatacc gcaataaaaa
    15601 aagtaaagaa taaaaccctt cttagaaaat tcctcattgt gctctcctaa atagttttat
    15661 ccttaacttt tccatttaaa acttgttcta ttcacttatt ttactgttaa attctatcaa
    15721 ttccttagca ttatttatag atttttcagt tattttcatt ccgctcaaca atctggcaat
    15781 ttcataaatt ttgttttctt tgtctaactt tttaactgta ctgaatgtat tattgtcaac
    15841 aacttttttt tcaatcaaaa aatgattttt tgaaattgaa gcaatttggg gtgaatgcgt
    15901 gacacagata acttgattgt tctttgcaat ataattcatt ttaacgccgg ccatctgtgc
    15961 agtcttgccg cttattccca tgtctatttc gtcaaacacc atggtttgac caatgtcgct
    16021 ttttattttt ttaaaagcta acattattct tgacgcttct cctccggaaa ctattttctg
    16081 aacggaattt aagtcgcttc ctacattagt ggaaattaaa aattcaacat cgtcaacacc
    16141 gtctgcagat atattttttt tattgatttt aacttcaaac tcaatatttt tcatattaag
    16201 ctcgcttaat tcgattaaaa tctgcttttc aagatattct gcagcggttt ttcttttctt
    16261 agatataata cctgcttttt ctatcaataa attatatatt tcattttttt ctgtaattaa
    16321 agaatttaat tttgaatcag ctttaagaag cagacttaat tcttcatggg atgcatttct
    16381 gtattcatta attttttcta ttgaaaaacc atattttctt tttaaacgat taataagaga
    16441 catcctgttt tcaatattat ccagtttttt ttcgtcgagg tccaaatttt ctgaatagtc
    16501 ccttaaatca tagcttaata gttttaattc ctctaactga atatccaccc ttgaaagaaa
    16561 ttcttccagt ttttcgtcat agtttttaat tttatttata atgctgtaaa cattgtttat
    16621 attatcgctg ttaagaattt gtattgcatc atacaaagat tttttaattt cctgtatgtt
    16681 agaaagcttt ttgatatttt taagaagctc ctcttcttca aattccttta actctgcact
    16741 atcaatttca tttatttcat attgtaactg ctcaattttt ttatctttat ttaatagttt
    16801 ttcttttaat gtgcttattt cgtttataac tcggttatat ttgacaaaca gctcattata
    16861 ggactgaagc aagggtgaca actcattttg tataagactg tcaagcattt taatatggtt
    16921 ttctttttta aataagcttt catgaccaaa ttgaccgtaa atgtcaatca ataaactgcc
    16981 tattgcctta atggtgttaa gaggataagt tcttccgtta accttgttta tacttttgcc
    17041 gttttgtaaa agctcccttg atattattaa aaagtcatcc ttatcaattt caatgtttaa
    17101 actctttaag tcttcataca ggctgtcatt atcgcctata tcaaaaattc cttctattaa
    17161 tgcagaattt ttgccttttc ttattagttc ttttgaagct ctatccccca aaataagctc
    17221 aattgcattt atcaaaaccg attttccgga cccggtttct cctgtaaaaa cattgaatcc
    17281 ggagtcaaaa tcaacagata cattttcgaa tactgcaaaa ttgcttatat ttaaactaag
    17341 cagcatatca tcaccattat cttatattct ttttaaattc ctgcaatacc ttatctacct
    17401 gttcaatttt tcgcacagct acaaagacgt tatcatctcc tgccaatgtt cctacaattt
    17461 cttcaattcc cataaaatca atggctgatg cacatatctg agcagctccc ggaattgttt
    17521 taataattat catattcatt gcattgtcga ttgaaacaac ggaattttca aatatcttat
    17581 tcaatctgtc cgttattcct tcctggcttt gacttatggt agcatactta tacttaccgc
    17641 ttttagacat tacctttaca agtctaagct cctttatatc tctggaaatt gtagcttgtg
    17701 taacatcaat acccatattt ttcagggctt cagctaattc ctcctgggtt tcaatttcac
    17761 tttttgaaat taactcaaga atttttgttt gtctggtata ctttttcatt tgacacctct
    17821 tagttttttt atagtttttc aaaggcttta tttattaagt ctttgaaatt aatttcattt
    17881 gtatttcctg taatttttaa gtgggcaaga tattctatgt ttcctgcccc tcctcttatt
    17941 ggagaataat ccatattaat taatttcaaa ttatttgttt tacaataatt attaatacta
    18001 ttaagtaccc ctaaatgtac ttctcttttt tttactatac cttttttccc tatgtttacc
    18061 ttacctgctt caaattgagg ttttaccagt gcgacaatat ctgtatcatt atgacttaat
    18121 tcgacaattt taggtataat atgttgaagc gaaataaaag aaacatcaac tgttataatg
    18181 tctataatat cagtaattat agatgtatca atatatctga aatttgtgtt ttctaaatta
    18241 acaactcgaa tatcattctt taatgaatca tgaagctgac cgcagcccac atctattgca
    18301 tatacttttt ttgctccatg ttttaacaga taatccgtaa atcctccggt tgaagcaccg
    18361 atatctactg ctgtcttatt ttcaatatta atgttaaata catgaattgc tttttcaatt
    18421 tttaaataac ccctacttat atatacatct tctgtaattt ttattttaac atcatcattt
    18481 accatatatg atggctttgt tattaattta tcatccacaa atacagaatt gtttaaaata
    18541 agctcctttg ccttttcacg gctgccaaca taccctttat tatgcaaaaa tacgtcaagt
    18601 ctgtaatttt tcatttttat gccttgcccg gtacataaat attcttctaa aaaaataaat
    18661 atttggatat tcaataaaat tatagcatat caattaactt atatcaatta atatttttaa
    18721 taattattaa aatttaagga tacaccttta tggaagtcag cctataatac aggtcttcaa
    18781 aggaatgttc cctcttatgt ctaaggattt cttattcaat attttcatct ataattttga
    18841 tttttctttg agtgtcatcc aactttttca tgcaaaaaga ataagccttc attccctttt
    18901 cataattttc cagaagctcg tcaattgaaa tatcatcttt attttcaaat ttccggacaa
    18961 tattttttaa ttcttccaag gcactttcaa aattatcgta ctccatatta cacctctttt
    19021 atatctcttg tttctgcttc tattttaaag tcagcaaatc ttatatgaag aatatctttt
    19081 aattctacct gttctttatg ctttataata tttcctgaat tgtccgatac caacgcaaat
    19141 cccttttcaa gctgtttctt gtaatcaaaa gtattcaatt tttctaacat tattttaaga
    19201 ttgaaataat actcattaat ttgtgaggta gttttatttt ttattaaatt tttataagta
    19261 tctaactgat tttttgtttt ataaatatcc tctaagggca ggctgcttct taataaatat
    19321 ttattagata ttagttcagc cttttttaat gatatggtat tgtttgtttc tgatttcaag
    19381 gtaaatacaa gattatcgag cttttcttca atatctttgt agtttctaat aattctttcg
    19441 gctgcagcag tcggagtatg acagtataca tcagccacat agtcggtcag tgttctgtct
    19501 gtttcgtgtc ctattccggt aacaaccaca atattggatt tataaacttc cattgctacg
    19561 tccatatcgt taaaaacatt taaatcctca aaacttcctc ctcctcttga aatgagaatt
    19621 acgtcagttt ccacactgtt aaaatatttt aatccttcaa taatactttt tttggatttt
    19681 tctccttgta ccaatgtgtt gtatatagtt atatcaatat tggcttttac attttcaaat
    19741 gtttttaata tatccttgat ggctgcccca gtctttgagg taattatgcc tattctttga
    19801 gggaattcgg gaagctctct tttgcggtca aagagtccct gctcttttag tttttctttt
    19861 aatagctcca aatctaaaag aattttacct aatcctgccc tttcaatatc tctaacaata
    19921 agctggtagg taccctttgc ttcgtatacg gaaagctctc cttccgctat tattttgtcc
    19981 ccgttttttg aaattgtgtc ggcaaaaaaa gcaacacaag atatacagga tatttcatcg
    20041 cataatgaaa aataagtata gcctgtctta cttattctaa tgtttgtaat ttcaccctca
    20101 accctcaaat tattcaggat tgaattactc attaagtact tttttatata ttggtttaaa
    20161 acagaaactt taattggttt taatttcatt gtgccacctg tcaataatgg ggagatatgc
    20221 aagacctaat gcattgtcgg aagaattatt tacagcagga aataatactt catatccttt
    20281 aacacttttt gtgatataat ttcttatata gctgttggat gcaactcctc ccgaaataat
    20341 taaggttttg tgttgaagat gtgacagact gcttattata tgtactatat attcggcaat
    20401 ataatcaaaa agtgaagtaa ttattacttc ctttgaatat ttatttagca gattgccaaa
    20461 atagttttcc attcctgata agtttatata tgaacctttt atcagtcttg gtcttgtaat
    20521 tttttcaaaa gttttgtctt taatgtattc ctccatatat tttccgcaag gaaatataaa
    20581 acccatcatg attccaagcc tgtctatgag ctgtccgaat gtaatatcct ttgtcccgcc
    20641 tacaatttta atatcgaagc ctttaatggc tttattgcaa gacaaaaatt ccgtggtacc
    20701 acctgatata tgcactgcaa ggaattcatc gatattttta taattgttta ttattgaagc
    20761 tgctatatgg ttttcctgat gagaatattc caaaaactcg caatttaatg tatcagaaat
    20821 aacctttccg aaatttcttc ctgcaataaa tacgggcata tatgagtttt cagcatttcg
    20881 aggttttgaa ctaactgata caatcttaat atttgaaagg tcatatggaa ttttttcgta
    20941 tagctccggc aagtttttta aatgctgaaa tacagcttct tgctgtctta ggcctttttg
    21001 tccttttttt acttcgagta cctttctttc atctgcaagt atttcattgc tttcttcgtt
    21061 aataagtgca acggatgttg tataagagct tgtatcaatt ccaagtatat atctcatttt
    21121 tttatgcacc ttactacagt acctaagatt ccgttaatga atttatggga gtcttctttt
    21181 gaaaattttt tgcttatttc cactgcttcg ttaattgata cactttcagg tattgtttca
    21241 ttatataata tttcagaaag agctatacgt aagactgcaa ggtctacctt tgcaatacgg
    21301 gagagcttcc agtccttgga attatcggta atatgtttgt ctatttcttc catattgtca
    21361 atcatatcgg atattgtttt atttaaataa tcaatatcca tgttttcaag ctcaaaatgc
    21421 tctaaatatt ctgtaatatt atttttgtta aaatctttgt ttacgtccat actgtatgca
    21481 atttttacag cttcttctct agtttcttta cgtttcatgt ttactcctga tactataaaa
    21541 gttttactgc cgcagaaggt atactcttcc ttatgcacca ttagggggac aatccatatg
    21601 gtgtgtcccc catcctctac tctattgcta tgttggcagc atccttttct ttttcttgta
    21661 ctttctcctg tattttttcc tgaacgatat taagtacgtg gacgtttaca gctgccacct
    21721 ttaagtctgt catagtttca atttctttct ttattgctaa ttgaacttct cttgctacct
    21781 catttatttt atagccatat tcaacattga taaaaatgtc cactactact gtatcttcat
    21841 taacatcgac ttttatacct tttgaataat tattttttga aaataaattg gtgattccgt
    21901 ctgttacacc tgtacgagta gtattgacgc ctttaacttg actcgctgct atacctgcaa
    21961 taattattac aacatcatcg gatattttta cctgaccgaa ttcaaatttt tcattgtcca
    22021 ttgtagcacc tcctaaaata attatagcag aagttgaaaa tatttacaaa tcaatattat
    22081 caaatttcac gatttttatt tgagaaggtg tataattagt ttctgaaacg actacatcta
    22141 aaataactgt ggcatctgcc ttttcaatcg tgtcatttgt tacaatttta accatgcttt
    22201 catctacaat ggcaactgat tcgttatacc cttttgattg aagcataagc tcaattccct
    22261 gttctgcttc aataaatgaa ttctttttca ataaaagctg ctcaaattgc tccttagttt
    22321 cgtcagatat caatgatgag ctcaaacttt cttcaagata atccaataca tccatgttgc
    22381 ttttttcctt gtttaattta aagttagcaa agctggcatt taaaacaccc aggacacctt
    22441 ccgaggaagc ttcctcagca gacgataaat ctgttatatc ccccaaaatt tcataatctg
    22501 tttcctccgg cagcacttct gcaaaatcaa cttcattaac tccgccctca attatttctg
    22561 caatgtcacc ttccaaatct gccggctcca tctggcctgt ttcaagatca tcaaatgcat
    22621 tatccggcgt caaactcata tttatatacc caataataaa tattattgcc agagatgcta
    22681 aaaatactaa tgtttctttt ctcattatca taagaccccc ttagtttata actattattt
    22741 caatatcatt taatttgata tccaataaag tttttacaga attgattaga atactgtaaa
    22801 tatctttatt tccgctgtaa tttactacca caagcacacc ggatatttca ggatattgat
    22861 aataccgctc aactactgaa ttatccctgc ctaatattac tttattttgt tttgtctctc
    22921 tgttcacctc tcttttagta ccgtcactca tagattcaat atttgtttca ttgctgatat
    22981 tttcatcata aacaggttcc agtatcggag tttgttttgt gtaaatcatt acatcaacac
    23041 tgttaatatc atcaatttta ctaagtattt cctccattct tttttctaat gcatatacat
    23101 agctttctgt tttcacttca ctatcttgat aacttacagg ctcccctgaa acttttgaaa
    23161 aatcaagttt taatattaag gatattatca ccagcaatat gaaaacatta actgcatagc
    23221 ttaatttttt gttttctttt agcattttaa aaaattcatt cataagcccc tccgtttatt
    23281 tctatcttat ctgagctaag ttgaaaaatt tcagctatgt ttgatttcaa tctatcgagc
    23341 tcatcttttc caatcatctt tcttaggttg actatggctg atatttgcat gtcctcttga
    23401 aatcttaaga taatatttga aacaacttct ccttggaaat tctctttatt aattgattca
    23461 ataatttcgg gagaaatgct ttttacgttt acctctcttt gaaaggcagg aacttcggtt
    23521 gttaccggca attttacatt catgctccca ttaagaaata tattatccag aatgaaaata
    23581 atgattaaaa gtttaataga tattctgcaa aatttctgtg tttttccctc cgggaaaatt
    23641 atcattatta aattaaaaat aagtattaat atcaaggtat taacaatcgt tgttttcata
    23701 gttatcctaa tatgttgttc atataattta ttattattga gtaataaaca gtgacaatta
    23761 ctaagggagt aataaggcat ataaggtaaa tatagaatat atccgccatt tcataaatca
    23821 gattggatat attttcatta aaaggttcag tcactacccc taatactttg tatataataa
    23881 gtgctatact aatctttatt attgagccgc caaatgatga cagaataaaa actattacta
    23941 caattccaag tacatctttt atcatcaata agctttttaa aaaaacaccg aagaaagatg
    24001 ttactgcatt tcccacaaca ggcaatgaag ccggagacat tgcctttaat gaacttataa
    24061 aaatattatc tgttttatat aatatatatc cttgtacaga aaaatttatg actacaataa
    24121 tcataaatcc ggttaaaatt aaaagggttc ctttttttaa tgccttgtac aaaagcttgg
    24181 cattaataag ctcattcatg ttgttggtta atataacagc tatcattatt accgtcataa
    24241 tatcgatgaa gctgtaaata aactgagttg taatccctat tgtatagtta agggaagttt
    24301 gaaagaactg cagcaaactc aacttaccga atgtaagcat tgctgccata aatattgcat
    24361 tgatttcttc cgtaatagtt ttaaaatcag taaaatcact tttaagtata cttttaatgc
    24421 tgagtacatc cttaagtatg atagtaaaaa taataattac cgaaaataat acaactatgt
    24481 ccgaaacact ttttccggta tcttcatcgg taaaatagtt tattatggtt agagctatgc
    24541 aaatgataaa cacattgata ccggttttca aaatatcttt gaaaaaactt tgttcttttt
    24601 ttaactcata gtacaaataa tccttcaagc tcacatctaa gtttcctgta aaagcgtttc
    24661 ttatcaagtc tcgtatacgt atgttatttt ctctgaaata cgcatttttt tcgtttatat
    24721 aatcttctat acccttagta ggaatttcat ccattgcttc gtccatggtt gaggctttgc
    24781 acgtaaaggt aaataatacg gtaaacacaa ttataaataa ggttattttt ttcattttat
    24841 cacccgttaa ctaagatcaa aatttgatct aaaaatattt ttataacatc aatggaatag
    24901 atgagtatta taagctttcc ggcaatttca acctttttcc ccacagattc ataatctaaa
    24961 tccttgcata aaagtgatat gaagtcacaa atgtaagcta taccgacaat ttttaatatg
    25021 gtaccgaaat attcagtctt tatatcatat tttacaaata aatctacaat ttcttccaaa
    25081 taaactctaa gctctttaaa aagcaggaaa attacaacaa taccgaaaat aactgttatg
    25141 tagactttga attcggagtt gaatttggat aaggttattc ccataattaa tgtaattaca
    25201 gctataagaa ttattttcat caaaagcatg atatccctct tcaatataag ttaaatacag
    25261 attttacctc cgtaaacagt tcattcatca ttcccacaat caaagccatt acaacaataa
    25321 ctcccgctaa ggtaacaaac atggcttgtt catctcttcc cgtctttgtt aaaatctgat
    25381 taaataccgc aactacaatt cctattgctc ctattttaaa tattaaatct atatccatat
    25441 tcccctccta agacattatg atagctgcca gacaacctgc aagaaatgaa attctgttgt
    25501 ttaatgtttt tttctcattg tgcttaatag aaaattcttt catagccctt tccatttctt
    25561 caatagtaaa tttaagcttt ctgtccaata cttccgaata tgtacaacca tagtaatcaa
    25621 ttatctctgt aaagtacgca ttaaaagcct ccggagttga cataatttta tttatgttaa
    25681 ccaaaagctc ttttgagctt tttttgtttt tcaaaatatt tatcatagac tttattatca
    25741 cttctgtttc tatgctttta aaattatatt ttaagcatac ctcttctata gacattttca
    25801 tatcacacga atataccctg agatattccg tgaaattaat taattccttc atttttttta
    25861 ttttttcatc accatcatgg tttattgagc tgaatatata cttaacggta actactgtgc
    25921 ctgcaaatag tgcaatccta atccaaatca ttgtaaatca acctgttctt ttgaaaatca
    25981 aatacttcat ttacttttaa gggtcgtttg tctaaaacaa atgctctttc aaagaaattg
    26041 ttatctatta tattttttag gtataaattt cttttcacat catccaaact aaacccgtgt
    26101 gcagttgcca caacttttat tccgcttttc agtgcatatt gtataatttc aaaatcctct
    26161 agagagccta attcatcaga aactattacc ttgggagaaa gtgcccttat actcatgaaa
    26221 aatccgtcac tttttttgca gtaggacaaa acatctgtcc ttctgcctgt attcatttgt
    26281 ggaataccgt tataaacggc gcttatttct cctctttcat cgattaaagt tacgtcacaa
    26341 ccttcaaaat acgggttttt aaagccgtcg cttaaatttc ttgctacgtc tcttattaat
    26401 gttgtcttcc ctgaaagcgg cggtcctata atcaatgtat tgtaaatatt tttatccgga
    26461 gttatgaaat gctttaatgc tttttcggag catcccggaa attcttttgc aattctaatg
    26521 ttaagagaag taatgttttt aaatccctta agcccatttt tgtcatagat gcagtctccg
    26581 cttataccaa ccctatgtcc tccttctgtg gttatgtagc ccttgtttat ttcgttttca
    26641 aatgcatgta ttgaattttt ggttaagttt cccaaaatat catcgatatc ttttttagta
    26701 ataacaatac tttcatctaa aaaaaactct ctttttagag ttttaataag taccggactg
    26761 ttaattctaa gcctgatttc aatgatttca tccaaaatac cgcaatctct aataataata
    26821 ttttttattc ttgtttctaa atggttcaat aaatttaaca tgttctctcc taccttgtcc
    26881 tcttaatcta taatattatt aatttaaaaa tttatgactt attttgtttc ttctgctgac
    26941 attcctttaa aactgatttt tagatttata gcttcgttca gaatcaccac atgtttacca
    27001 taacacgtta aatgaatcac tttgtcatcc tgagcgaaag aggatgtttt tataagattc
    27061 gccaaaaaaa tatcaaattt ttttaaaaaa gaggtttaca aatatataat taatggtata
    27121 atagtcatgt aaatatcttc agggcagggt gaaattcccg accggcggta aagtccgcga
    27181 gccgaaaggt tgaattggtg agattccaat accgacagta aagtctggat ggtagaagag
    27241 attacttgca tcttgtaagc aaattcttta aaatgcctct gaagatttca gaggattttt
    27301 atttgcctct gacaatctta aaatcgtgct gagcacaagg aggaaaatat gaaaagtgac
    27361 gtaacaatga atcaaagaat taacacaaaa actattacaa aggtaggaat attaggagcc
    27421 gtagcgacag tattaatgct tttagagttt ccgctatggt tcgcaccaag cttttatgaa
    27481 ttagacttca gtgaagtacc ggtattatta ggagcatttg ccttaggtcc ggcagcaggc
    27541 gccatgatag aattggttaa gattttaatt aactttgttt taaacggaac agataccggt
    27601 ggagtaggag aattcgctaa ctttataata ggatgcgcat tcattgtacc tgcaggcttc
    27661 atttataaaa gaaataaaag ttttaaaacc gctattttgg gattagttgt cggaacattg
    27721 accttagcat tagcaggagc atttttgaat tattatctgt tgcttccgct atatgcaaaa
    27781 atctacggct atccaatcca ggcatttata gatatgggta atgctttaaa tccggcaata
    27841 acagatttaa aaacatttgt actgtatgcc gtagtaccct ttaacctact aaagggcaca
    27901 atagtttcgg ctatggtaat attgatttat aagaagcttt ctccaatact gcacagataa
    27961 ttgaagatcg atgcaggaaa atgtcgtaat taatcaattg ccacagacgc atataaaatg
    28021 cgtctttttt tgtatttctc taataaaagc tgattatttt ttgaccgcag agcgcgcaat
    28081 atatgcttta attgcgcttg actctaatac tct
//
LOCUS       DAOPUE010000014        27668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1704_length_27668_cov_9.252345, whole genome shotgun sequence.
ACCESSION   DAOPUE010000014 DAOPUE010000000
VERSION     DAOPUE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 27668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27668
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1704_length_27668_cov_9.252345"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKH42_02530"
     CDS             <1..655
                     /locus_tag="PKH42_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19335.1"
                     /translation="IIAFAIGIAIGAVLAWKRNTKFDLVGTIIALIARCIPAFWTGMI
                     LLLIFSYKLNFLPAGGMHTPGVRFDSIVQKYFNLDFLKHMILPTITSAFYSLATPLLV
                     MRTSMLEIVKEDYIEMAYAKGLQEKYIIKKHAVRTALLPVITIFAVQAGAAIGGAVLI
                     ETVFRWPGMGREIVLAVSYRDYPLAQAAFLLIGLMTALFNLIADILYALLDPRVALS"
     gene            670..1521
                     /locus_tag="PKH42_02535"
     CDS             670..1521
                     /locus_tag="PKH42_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19336.1"
                     /translation="MKKIKRFLKGNIAGLVGFFIFIFFVIIAVTADYLAPYDPMEILY
                     NDDGTVKRLQPPSKEHILGTTYMGRDVLSQLIVGSRVAVLVGTISAICVAFIGTNIGL
                     IAGYYGGVTDNLIMRFVDVVYGIPFLPFSLILVAILGPGVKNIILAIVLITWRNSSRV
                     IRSQVLSLKNRTFIEAARLSGAGNARIIYKHIAPNVIPLSMVYVSTTMASAIMTESSL
                     SFLGFGDPTQMSWGRILFSCYSSQAMFKAWWWMIPPGIAIAFLVLSGYLMGRAFEELA
                     NPKLRER"
     gene            1566..2591
                     /locus_tag="PKH42_02540"
     CDS             1566..2591
                     /locus_tag="PKH42_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19337.1"
                     /translation="MKLLDIKDLSILYKTVEGDIRAVDKVSLALDDREILGIVGESGC
                     GKTTLVKGIMGLLPHNAKISGGEIYFNNTDLLKLTYEQMRQIRWKDISMITQSAMNSL
                     NPVYKVGWQIVEVIRAHTDVSKEEAYKKVEELFELVGIEKGRMNNYPHQFSGGMKQRA
                     IIAMALALDPKLIIADEPTTALDVVVQSQILQRINHVLEKYNGSMILVTHDISVVAQT
                     CNMVAVMYGGVIVEYGPVKQVLKYPNHPYTMGLNNAFPSLTGEKRQLISIPGSPPNLM
                     KDLKGCGFKDRCPFAVQKCFIDEPKQTSIIKGFYYCHRNDEIDKLRGLAKKQEIWHEE
                     FLKEVTI"
     gene            2588..3589
                     /locus_tag="PKH42_02545"
     CDS             2588..3589
                     /locus_tag="PKH42_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19338.1"
                     /translation="MNITKKNQFIIEAFNIKKEFMVKIKKNALTSEEKILKAVDGIDL
                     SVNPGEILGIVGESGCGKTTTAKLLLGLYKPSSGCIKFKGEDVSEFRGQKIKDFRRNA
                     QMIFQDPYESLNPRFKIEEVLIEPLKIHNIGSNRKERYELAEKILYDVGLKPPKTYLE
                     RYPHEMSGGQRQRVSIARALILNPSFLIADEPVSMLDVSIRAGIMNLLTRMIEERNLA
                     SICISHDLSLIRYVTEYTAVMYLGKVVEKASTENLIKKRYHPYTEALMEAVPNPDPDY
                     KTNISKIRGEAPNPVNVPTGCRFHPRCIKSMDICKEKEPREIEIENNHFVLCHLYNK"
     gene            3626..4378
                     /locus_tag="PKH42_02550"
     CDS             3626..4378
                     /locus_tag="PKH42_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOA19339.1"
                     /translation="MKALILEEITTSELKKYIDNIKIAVVPTGSCEQHGPNTTFATDT
                     VRAYEIAKLLGVKYGKDLLICPPIGYGISPHHMNFTGTITLRIETYVSVMMDIAWSLK
                     EHGIKKILFLTGHGGNRNALSTVITKIKMEYNIEAYWAGTGSGLVKDLLDKKGCSEIR
                     GHACEAETSQSLYLAPWLVKEDKLEKGKLIKDNKYIYKEIVRDGGVFWDFSSITENGA
                     LGDATKSSIELGKEITDLVIDRLSDMIDKIIL"
     gene            4769..5464
                     /gene="ftsE"
                     /locus_tag="PKH42_02555"
     CDS             4769..5464
                     /gene="ftsE"
                     /locus_tag="PKH42_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOA19340.1"
                     /translation="MIEFNNVTKIYKKTFTAVKNVNLKIDKGEFIFIVGKSGAGKSTL
                     IKLLLREISPSEGIITYNNQDINKIRKRSVAEYRRRIGFIFQDYRLLPKMTVYENIAF
                     AMEMICCQAKNIRREVPLVLSMVGLSDKAKYYPDELSGGEQQRVAIARAIINKPETVI
                     ADEPTGNLDMETSKEIMKILTEINRRGTTVIMATHDREIVNQYQRRVVELKNGIVIRD
                     IKAGGYSYESEED"
     gene            5445..6350
                     /gene="ftsX"
                     /locus_tag="PKH42_02560"
     CDS             5445..6350
                     /gene="ftsX"
                     /locus_tag="PKH42_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOA19341.1"
                     /translation="MNPKKISYIFRQAFKSMWRNRMMGFASIGSVSAVLIILGFVLIL
                     VLNINNMAMVAKETFDQIAVYIMEGVDDEQIEEMGNSFRKIDGVMGVAFQTKDYALEK
                     VKEDWGEDAILLQDLRHNPFPNTYIVQLSDVSKADKVIAQLQTFEGVDKVRYYQDAVN
                     TLIKLADFVKKFGAGIIIILLLISVFIISNTIKITVLSRQREIELMQYIGATNGYVRG
                     PFILEGVMLGLLGSVLAILLIMLGYNYTIDYLAGRYVALISGMSGYLVGVEMVLYDLI
                     IIFMTIGIGIGVLGSLVSLKKFLSI"
     gene            6361..7485
                     /locus_tag="PKH42_02565"
     CDS             6361..7485
                     /locus_tag="PKH42_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOA19342.1"
                     /translation="MFRKCVAVILLIITIIGIFPANAGGSIADLRYKQKQIEQQIKDI
                     RKKAEELKGEKKSIEAEIQILDSELRALTLETEKQEVQKQELNMRIAESEQKIAELTD
                     EIDKNNMLLEQRLRAMYMNGTGGYLEVILNAENLIDALTRMDLIQLIVKSDVELLKTI
                     NEQKKEVEELKAAQEIRRHELTAVINDLNIKQNEVLIASRSKENYMSSLQNNIEEILR
                     QEAAMEEQSKQIEKDIIALQRAVEYAGGELLWPLPGHYRITSEYGGRIHPITGVWSTH
                     GGTDIAAPYGTNILSSNDGVVIYAGYHYSYGNYIIVDHGGGIATLYAHCSKLLASEGQ
                     AVTKGEPIAKVGSTGESTGNHLHYEVRLNGVRKNPMEYLK"
     gene            7557..8594
                     /locus_tag="PKH42_02570"
     CDS             7557..8594
                     /locus_tag="PKH42_02570"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOA19343.1"
                     /translation="MRIGIVGFGGVGRAFLNLIYNKKNKLESEGIYFQVNYIIGRTGG
                     VYNKHGIDLHKFINFLFDEKDITKYCDGGKAEDINFELMLNNKDIDLMVEMTPTNKET
                     GEPGMTHITRCLENNINVVTSNKGPILLAYHKLYNLAKANKVQLGIGCTTGGALPSIN
                     GGFIDLAGADIISIEGVLNGTTNFILKEMEDTGCNYDDALKEAQRLGIAETNPALDVE
                     GFDTASKLLILTNVLMNTEKTMDDIFIEGITKLTPHDINRAKSMNKKYKLVGKTQILD
                     NKIEMEVKLQLLDPSNPLYGVEGKNKAVRYISDTLGELTIMGGASGVTPAAASILRDI
                     ININRGYKFVK"
     gene            complement(8715..10265)
                     /locus_tag="PKH42_02575"
     CDS             complement(8715..10265)
                     /locus_tag="PKH42_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19344.1"
                     /translation="MKIKYFPLIIFFIVYILLFATLPQNIKLPLLYFTPLLLYTMFIS
                     KAMFYYMKNKKQDDDMEVNEIASTVTYFDKKKIDEQKIQISFNDVAGLEEIKEDLMDI
                     IDFLNNEDKYRLMDAKVPRGILLYGPPGTGKTLIAKAIAGEAKATFIYSSGSEFVEKY
                     VGVGAKRVRTIFEKARKEAPSIIFIDEVDALGAKRNADSNNEKDQTLNQLLIELDGFN
                     NTSNVIVIAATNRLDLLDEALLRPGRFDRKIYVGNPNFHSRLKILEVHTKNKPLDKKV
                     QLKDIAIKTHGFSGAQLANIANEAALKAIKEKSKKITNENFEFAIEKIAAGLEIKHST
                     VTDKEKRIVAYHEAGHAVAAKILNIDQVQKISIIPRDKALGYVLKFPTEDKFLYTKNE
                     LCNKVIVLLAGRASEEIFIGEISTGASNDIKESTNIIYEIICNYGMGKNTQNTCIDER
                     FIKYYLDNIKDEAQEMTKEAYESAMAIIRENEAVVDKIAQYLLKYETMNAEKLDELMD
                     ETKKTALI"
     misc_feature    10396..10519
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            10619..11128
                     /gene="infC"
                     /locus_tag="PKH42_02580"
     CDS             10619..11128
                     /gene="infC"
                     /locus_tag="PKH42_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOA19345.1"
                     /translation="MIKELQINEQIREKEVRVVDSDGSQLGIISTKEALKIAESKKLD
                     LVNVSPNSDPPVCRIMNYGKYKYAQAKKEKESKKKQKVISVKEIRMTPNIEEHDLAVK
                     AKNASKFLIDGDRVKVVVRFRGRELGKMDRGREVLMQFAEMTSEYSTIDKHPVVEGKN
                     MSMFLTPKK"
     gene            11146..11340
                     /gene="rpmI"
                     /locus_tag="PKH42_02585"
     CDS             11146..11340
                     /gene="rpmI"
                     /locus_tag="PKH42_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOA19346.1"
                     /translation="MPKIKTNRSAAKRFKKTGSGKIQRAKSGKNHFTGKKASKSIRNL
                     RKGTLVSGADEKKVNKLIPY"
     gene            11365..11715
                     /gene="rplT"
                     /locus_tag="PKH42_02590"
     CDS             11365..11715
                     /gene="rplT"
                     /locus_tag="PKH42_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOA19347.1"
                     /translation="MARVKGAVNAKKNHRKILKLAKGYYGAKSKLYKTANQAVMKSLS
                     YAYVGRKLRKREFRQLWIARINAAARQNGLSYSKFINLLKVNNIEINRKMLSEMAIHD
                     PEGFTNLVEQVKIS"
     gene            11821..12612
                     /locus_tag="PKH42_02595"
     CDS             11821..12612
                     /locus_tag="PKH42_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOA19348.1"
                     /translation="MDIITSKDNIKIKYIRSLNLKKFRDEENAFLVEGIKFVEEAINE
                     NADIKLLLVSEETLNKSEVKKLIDSIDESKAVVCTAQVFNSAADTVNAQGVLAVINKN
                     TAFQNDIIDKFNFIIMCDRIQDPGNLGTIIRTADAFGPAALLINKGCVDIFNPKVVRA
                     SAGAIFRVPLIHSKDDLEVINIMKDKGFYIVSTVVDSEYYIDNIEKRNKICIVIGNEG
                     QGVSQDIIDNSDISVTIKMTGKAESINASVAAGICIYEIRKKLLK"
     misc_binding    12671..12900
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12936..13970
                     /gene="pheS"
                     /locus_tag="PKH42_02600"
     CDS             12936..13970
                     /gene="pheS"
                     /locus_tag="PKH42_02600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOA19349.1"
                     /translation="MKEQLLKLKTEALNRLKECNDNADIEKIRIQYLGKKGELTSILR
                     SMGSLSEEDRPEMGKLANEVRALIEEAIQSTKETVHERESEKRLKTESLDITMPGRQP
                     EIGRRHPLMRVKEELESVFMRMGYDVIGGPEIETVANNFDELNAPYDHPSRDWSDTFY
                     ISEDLVLRTHTSPVEIRVMKEAVREGKLPIRMVSTGRTFRFDDVDDTHSPMFHQMECL
                     VVDKGVTMANLKDSIDQFVRELFGSNVKTRFRPHNFPFTEPSAEVDVSCHICMGKGCA
                     ACNGTGWSMELLGCGMTHPNVLRNCGIDPEIYSGYAFGMGIDRVAMVKYGIPNIRLLF
                     ENDVRFLKQF"
     gene            13981..16374
                     /gene="pheT"
                     /locus_tag="PKH42_02605"
     CDS             13981..16374
                     /gene="pheT"
                     /locus_tag="PKH42_02605"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOA19350.1"
                     /translation="MLVPIKWMKKYVDIDVDDKTLADKLTLTGSHVDAVIDYNKEVKR
                     VVTGKILEINQHPNADKLVVTLIDINESEPVQIITGAKNIKKDDVVPVALEGAELPNG
                     LKINKTNFRGLPSYGMMVSYEELGFEDKVIPKESRDGIIILPSDTPLGVDIKEILELD
                     GSALEVEVTFNRPDCLNIVGMAREAAATLGTQLKFPEIKINNEEGNIKDYLNGVEIED
                     EDLCSRFYARVIKDIKIGPSPLWMQRILMDAGMRPINNIVDVTNFVMLELGQPIHAYD
                     LEKLEGRKLIARRAKDGEKIVTIDQNERELDNNMLVIADSKNPACIAGVMGGYYSEIS
                     DDTKIMALETANFNPKSIRETSKKIGLRSEASARNEKPIGYVLCEIASNRVCQLIEMI
                     GAGTVVGGYLEAGKNEYEPPVVTLRPHRASSLLGVEIPVADMLKFLNTLEIESVYDGE
                     KITSRIPYFRTDIEQEADLIEEVGRLYVFENIESKPLRGSTRVGRKSDLRMVEDEIKS
                     ILTGMGLYEITTYSFISPKTYDSIYAAEDSPLRNYVRILNPLGEDFSSMRTTLMPNML
                     EVIQRNSNRGVEGAKLYEAGSIFIPRDQPVAEEPFEKLRLCIGMYGSYDFYDLKGIID
                     AMLSRFGIKAALKKVSDNPTFHPGRTAALCVDDDIIGIYGEVSPKVTDNYGLDHRVYL
                     AEIDVEKILQYKNTEWKYEPLPKYPSMVRDIAVIVKDDVLAGDMEEEIRKVNPYLIES
                     VELFDVYKGEHIKEGYKSTAFSVTYRNKERTMKEKEVEKIHEKILQVLSNKFNAVLR"
     assembly_gap    16463..16562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16708..17442
                     /gene="lgt"
                     /locus_tag="PKH42_02610"
     CDS             16708..17442
                     /gene="lgt"
                     /locus_tag="PKH42_02610"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOA19351.1"
                     /translation="MNPVAFNIFGIDIMWYGVLISAGVLIGVLIALKEAKRTGFREDD
                     LIDFLLYAIPAGIIGARAYYVIFSWDYYSQNPSQIINIRSGGLAIHGAIIAGVVTGIL
                     FCKIRKIDVLEILDLVMPSVALGQSIGRWGNFINQEAHGGPTDLPWGIIVDGQKVHPT
                     FLYESIIDFCIFLFLIWFRKKKKASHGQILGLYLILYSAGRYFVEGLRTDSLMFMGMR
                     VAQLISIASIVIGATLLIYLRNKKTI"
     gene            17609..17890
                     /locus_tag="PKH42_02615"
     CDS             17609..17890
                     /locus_tag="PKH42_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOA19352.1"
                     /translation="MYYVYILTNKYNKVLYVGITNDLIRRIYEHKNNLVEGFTSKYNV
                     HKLVYYDLTSDVMSAITREKQIKGWTRDKKIKLIESMNPEWKDLYYDII"
     gene            18295..18732
                     /gene="mraZ"
                     /locus_tag="PKH42_02620"
     CDS             18295..18732
                     /gene="mraZ"
                     /locus_tag="PKH42_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOA19353.1"
                     /translation="MFTGEYIHSFDEKGRVIIPSKFRNELGETFYIGKGLDKCLFVYP
                     IETWTEFVGKLKNLSTFNKEERFFLRRFVSGFSECSFDKQGRILIPPQLRSFSELNTD
                     AAIIGVIDRIEIWNKDTWDEYSNNEQFDFDSIAEKMSELGIGL"
     gene            18742..19671
                     /gene="rsmH"
                     /locus_tag="PKH42_02625"
     CDS             18742..19671
                     /gene="rsmH"
                     /locus_tag="PKH42_02625"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOA19354.1"
                     /translation="MDYIHKPVLLDESVKGLNIKEDGIYVDCTLGGGGHTEEILKKAT
                     KGKVIGIDQDDYAIERASEKLSGYKNFMPVKNNFSKIEEILNELSIDKIDGIIFDLGV
                     SSFQLDIPERGFSYNYDMPLDMRMDKSQTTTARHIVNGYEENELTRILWDYGEEKWAA
                     RIAKFIVEEREQEFIETTGQLVSIIKKAIPKSARQEGSHPAKKTFQAIRIEVNRELEI
                     LENTVKAAVERLTKKGRICVITFHSLEDRIIKNTFAELNKDCICPPEFPKCMCEHRRK
                     LKIITKKPITPSEEELTENRRAHSAKLRIGERV"
     gene            19675..20139
                     /locus_tag="PKH42_02630"
     CDS             19675..20139
                     /locus_tag="PKH42_02630"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOA19355.1"
                     /translation="MSALRKEAVELHEHELEKEYSPKKRIKRNKRNNTFIESLKNSVM
                     IAITFILGILIVYNYAVITDKQMELNNLNIEIENLNNEIDKYNIALESIKNTNSIEEM
                     AKVYLGMNYPTRKQTVFVDFTYGPDAIEEKNIMAKDENLLVGLIDKVISFIR"
     gene            20153..22270
                     /locus_tag="PKH42_02635"
     CDS             20153..22270
                     /locus_tag="PKH42_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOA19356.1"
                     /translation="MKKRAKSTTNLQNKRRMLFFLVCFFLVTLALVVRLGYIQLIKGE
                     EYRKQAMENWSRDVTISAKRGTIYDAKGKKLAVSINSNTVTCFPADVKKSVNPTVDNS
                     IDEDDSILTAILKKLNRAMFTEETQAEQIPVNTKTPEEIAAILAEILEMDYDEVLEKI
                     TSNYNYVVVKKWITDEQAQKIREQELSGISIIEDNKRVYPYGNFAPYVLGFTNIDQDG
                     LYGVEATYNSYLTGIPGRTVVNTDAYGRELPFGYNEYYESQDGLGVVLTIDEVIQHYA
                     ESAAEKVMADYNAKRATIIVMDPNTSDILAMASKPDYDPNDPRTPIDENIKAEWANLS
                     NEELQNAWFDMWRNPAVNDVYEPGSTFKLITTATALEENVATLGSTYFCDGYVRQITS
                     HNIKCWRYYRPHGQQTLSEAIQHSCNDALAQIGLDIGKDVFYKYVKSFGFEEQSGIML
                     NGEATGIVKKPEYMKDVDVVTQAFGHGVAITPIQLINAVSAISNGGNLMVPRIVKQLI
                     DEDGNIIKNFDTEVRRKVISEETSKIMLQIMKDSAEAGTKQAYRPGYRIGGKSGTAQK
                     VIDGKYIEGKFISSFIGVAPVDNPRAVALVMVDEPEMSIGYYGSIVAGPVAADLLENI
                     LKYYDVEPVYTEEELGHVEKQMVTVPNLLGLTVAEATDKLISAGLESNITIEVDAERT
                     VKSQFPKAGEQVVKSSMVTLTLN"
     gene            22411..23895
                     /locus_tag="PKH42_02640"
     CDS             22411..23895
                     /locus_tag="PKH42_02640"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOA19357.1"
                     /translation="MNIKNLLKEMDYLSYIGEDDIEIRGITNDSRKVNRNDIFVAIKG
                     FTNDGHKFIENALENGASAVICENIPENVKGKSNFIIVKSARASMAKAANIIYGRPSE
                     KMKIAGVTGTNGKTSTTYLLNGIYDYLGVKSGIIGTMGVLIEGKKIKVDNTTPEAFDI
                     QHYLAMMLEKDITHCCMEVSSHALELNRIDEVKIDVGIFTNVTRDHLDFHKTMENYYQ
                     AKKKLFYLTKVNNIINVDDPYGLRLYNELIKEGIKAISVGIENDADIKASNLKTTMNG
                     TTFNLNVMGKEKTAHVNTPGTFSVLNSLGALGAAYALGGNIDDIIEALKQIKGVTGRF
                     EILPNKLDCTIILDFAHTPDGLQKVMETINEFAEGRKIVMFGAGGERDLSRRAPMGEI
                     AGKYCDLSILTSDNPRFEDPYEICKEIAEGVKKSNGEYKIIVERDQAIYYAIDKSQKK
                     DVILLAGKSTEPYQDMGTEKVPYDETMLAKRTIKEVEKKRGLSS"
     gene            24041..25015
                     /gene="mraY"
                     /locus_tag="PKH42_02645"
     CDS             24041..25015
                     /gene="mraY"
                     /locus_tag="PKH42_02645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOA19358.1"
                     /translation="MQGNGQIIRVIIVSFLIALFLGPVVIPVLKRLKVGQSIREDGPQ
                     SHLAKSGTPTMGGIIIILAILTSIITGRLITKEVLAVIFFMLGFGLIGAIDDYKKVVM
                     RQNEGLKPYQKIVGQLIFSVLLAWYGSRYSAMKTALMIPFVNMHIDLGVLYIPFITFI
                     AVGTVNAVNLTDGLDGLNTGVTLIVMAAFSLIANSLKETNQIYEGISLICAATSGACL
                     GFLKHNANPAKVFMGDIGSLALGGVVSSVAVLSGMVLFIPIIGGIYFVEALSVIIQVF
                     HYKRTRRRVFKMAPLHHHFEMCGWKETKVVSAFWIATLILAFIGIYSI"
     gene            25057..26421
                     /gene="murD"
                     /locus_tag="PKH42_02650"
     CDS             25057..26421
                     /gene="murD"
                     /locus_tag="PKH42_02650"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOA19359.1"
                     /translation="MNVLVVGLGVSGIACIKGLSKTGANIYAFDDSNEEKLRDRLKEV
                     EGIEVEYFFGNEEINKIKFGNLDYAIKSPGIKYETPIIEKLVKNNIEVISDIEAAYRV
                     TNSPIVSITGTNGKTTTTTLIGEMVRESKKTYKVTGNIGFGIYYDALTAKEEEILVAE
                     VSSFQLAGTSKYKPHISVITNITPDHLDYHHTVENYIEAKFKNVINQDENDYAILNYE
                     DETIRNHSDKIKAKKIFFSSERILEDGIYSENGQMFYRTGNNKLSIINTKDIFIPGKH
                     NLENAMAAGGAAIALGIDIEVISRVLREFKGVEHRLEFTGEYKKVKFYNDSKGTNPDA
                     SIKAIQGIEKPIILIAGGYDKKSSYDDFIKAFDNKVKALILLGQTAEDIQKCALKYGF
                     ENIYRVETMDEAVKKSFELAEEGDNVVLSPACASWGMYPNYEVRGRDFKERVEYYGRN
                     RKKS"
     gene            26396..27517
                     /gene="ftsW"
                     /locus_tag="PKH42_02655"
     CDS             26396..27517
                     /gene="ftsW"
                     /locus_tag="PKH42_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOA19360.1"
                     /translation="MEETVRKAKARSIDWVLVFIIVLLVLFGFLMVYSSSYPDGYYKY
                     GGDPFYFLRKQVIAAGAGLAGMIFFANLPYIIYKKFSKLILFTCIGLAMLTIIIGIAS
                     NGAQRWIEIGGISLQPSEIIKIGAVLYMADYFNRKRSELPSFTKGFLPSLFYIVLFCG
                     LIAIQDDLSTAVILAGTLMVMFFCAGARISHLILLFLLALAGIIALIAMQTYRISRII
                     VFFDPFKYPLDEGYQIINSLYALGTGGLFGMGVGKSRQKFFHLPEAYNDFIFSIIGEE
                     LGFVGAVFVMLGFVVFAWRGLRISMNTEDFFGSQTALGLTVIVLVQFLIHVAVATSSM
                     PPTGRALPFISAGGSSIMFLLCSMGILLNISKYSNTYRS"
BASE COUNT         9778 a         4143 c         5767 g         7880 t
ORIGIN      
        1 tattattgcc tttgcaatag gaattgcaat aggtgcagta ttggcatgga aaagaaatac
       61 aaaatttgac ttagtaggaa caattatagc cttaattgcc agatgcattc ctgcattttg
      121 gaccgggatg atattactat taatattttc ttataaatta aattttttac cggctggggg
      181 catgcataca cccggtgtaa gatttgacag cattgtgcaa aagtatttta atttagattt
      241 tttgaagcac atgatccttc caaccattac ttccgcattt tattcactgg ccacaccact
      301 gctggtaatg cggacatcaa tgttggaaat agttaaggaa gattatatag aaatggctta
      361 tgcaaaagga ttgcaggaaa aatatataat aaaaaaacat gccgtgagaa cagcattgct
      421 tcccgtaata acaatttttg cggttcaggc aggtgctgca attggtggag cagtgctgat
      481 agaaacagta ttcagatggc cgggaatggg cagagaaatt gtgcttgctg tttcctacag
      541 agattatccc ttggctcagg ctgcatttct tctcataggt ctgatgacag ctcttttcaa
      601 tcttatagca gatatattat atgcattatt ggacccgagg gtagcacttt cataaggagg
      661 attattataa tgaaaaaaat aaaacggttt ttaaaaggta atatagctgg tcttgtcgga
      721 ttcttcatat ttattttctt tgtaataatt gcagtaacag ccgattattt agctccttat
      781 gatccaatgg aaattctata taatgatgac ggcacggtta aaagattaca accgccttct
      841 aaagaacata ttctcggaac cacttatatg ggaagagatg ttttatcgca attaattgta
      901 ggctcaaggg tagcagtact ggtaggcaca atatctgcca tatgtgttgc atttataggc
      961 acaaatattg ggcttatagc cgggtattat gggggggtta ccgataattt aattatgaga
     1021 tttgttgatg tagtgtatgg aataccattt cttccttttt cgctgatatt ggttgctata
     1081 ttgggaccgg gagttaaaaa tattatattg gctatagtgc taattacatg gagaaattca
     1141 tcaagagtta taagatctca agtattgtca ttaaagaaca gaacctttat cgaagcagca
     1201 agattaagcg gcgccggtaa tgcaaggatt atttacaagc atattgcacc taatgttatt
     1261 cctttatcca tggtttatgt ttccacgact atggcttcag cgataatgac cgaatcaagt
     1321 ttaagctttc ttggattcgg agatcccaca cagatgagct ggggaagaat attattttca
     1381 tgttattcat cccaggcaat gtttaaagca tggtggtgga tgatccctcc aggtatagca
     1441 atagcgtttc tggtattatc aggatatttg atgggaagag cttttgagga attagccaac
     1501 cctaaactga gggagagata gattgaataa ttcagataat aacaatcttg aaagggcata
     1561 tatgtatgaa attattggat attaaagatt taagcatttt atacaaaaca gttgagggag
     1621 atattcgagc agtcgacaaa gtatctttag ctttggatga tagggaaata ttgggaattg
     1681 tcggggaatc aggatgcggc aaaactacgc ttgtgaaagg aataatgggc ctcctgcctc
     1741 ataatgccaa aatctccgga ggcgaaatat attttaacaa cacagattta ttaaaactaa
     1801 catatgagca aatgagacaa atcaggtgga aagatatttc aatgataact caaagcgcaa
     1861 tgaattctct aaatcccgta tacaaggtcg gatggcaaat tgttgaagta atacgtgctc
     1921 atacagatgt atcgaaggaa gaggcatata aaaaagtaga agagttgttt gaacttgtgg
     1981 gtatcgaaaa gggaaggatg aacaattatc ctcaccagtt cagcggcggg atgaaacaaa
     2041 gagcgattat tgcaatggct ttggctctgg accccaaact tataattgca gatgaaccga
     2101 caaccgccct tgatgtagta gttcaatcac aaatacttca gagaatcaat catgtactgg
     2161 aaaaatataa tggatctatg attcttgtaa ctcatgacat atccgttgtt gcgcaaacct
     2221 gtaatatggt ggcagttatg tacggcggtg tcatagtgga atatggtcct gtgaaacagg
     2281 tattgaaata tccaaaccat ccatatacta tgggattaaa taatgcattt ccaagtttaa
     2341 ccggtgaaaa gagacagttg atatctatac ccggttcacc gccaaatctt atgaaagatt
     2401 taaaagggtg cgggtttaaa gacaggtgtc catttgctgt gcaaaaatgt tttattgatg
     2461 aaccaaaaca aacaagtata attaagggat tttactattg tcatagaaat gatgaaatag
     2521 ataaactgag agggttggca aaaaaacaag aaatatggca tgaagaattt cttaaggaag
     2581 tgacaatatg aatattacaa aaaagaatca gtttataatt gaggctttca atataaaaaa
     2641 agaatttatg gtaaaaataa aaaaaaatgc tttaacttcc gaagaaaaaa tattaaaagc
     2701 tgttgatggc attgatttaa gtgtaaatcc cggggagata ttagggatag tcggggaatc
     2761 gggatgcgga aaaacaacta cagctaaatt gctgttaggt ttgtacaagc cgagcagcgg
     2821 gtgtatcaaa tttaaaggtg aagatgtctc ggagtttaga gggcaaaaga ttaaggattt
     2881 cagacgaaat gcacaaatga tattccagga tccctatgag tcattaaatc caaggtttaa
     2941 aatagaagaa gttttaattg agccccttaa aattcataac atagggagca atcgcaagga
     3001 aaggtatgaa ctggctgaaa aaatcttata tgatgtagga ttgaaacctc caaaaacata
     3061 tttggaaaga tatcctcatg aaatgagcgg aggtcaaagg caaagggttt ctatagccag
     3121 ggcactaata ttaaatcctt cttttttaat tgcagatgaa cctgtatcta tgcttgatgt
     3181 atcaataagg gcaggtataa tgaatctact taccaggatg attgaagaaa ggaatttagc
     3241 ttcaatctgt atctctcatg atttgtcatt gataagatat gtgactgaat ataccgctgt
     3301 catgtatctg ggaaaagttg ttgaaaaagc atcaacggaa aatctaataa agaaaagata
     3361 tcatccttat acagaagcac tgatggaagc tgtgccaaac cctgatccgg attataaaac
     3421 taatatatcc aagataagag gtgaagcgcc aaatcctgta aatgttccaa ccggttgcag
     3481 gtttcaccca agatgcataa aatccatgga tatatgtaaa gaaaaggagc cccgggaaat
     3541 tgaaattgaa aataaccact ttgtattatg tcatttgtat aataaatgag cagtagttat
     3601 aaatattata gaaaggactt ataaaatgaa agcattaata cttgaagaaa taacaacaag
     3661 tgaattgaaa aagtatattg acaatataaa aatagcagta gtaccaacag gttcgtgtga
     3721 acagcatggc ccaaatacta catttgctac cgataccgta agagcatatg aaattgcaaa
     3781 attattagga gttaaatatg gaaaagactt attgatttgc ccacctatcg gatatggaat
     3841 ttccccccat catatgaatt ttacggggac cataacactg aggatagaaa catatgtatc
     3901 agttatgatg gatattgcat ggagcttaaa ggaacatgga ataaagaaaa tattattttt
     3961 aacaggacat ggcggtaaca ggaatgcgtt aagtacagtt ataacaaaaa tcaagatgga
     4021 gtacaatatt gaagcatatt gggcaggtac aggttcaggc ttggttaagg acttgcttga
     4081 taaaaaaggg tgttcggaaa tcaggggaca tgcctgtgaa gctgaaacgt cacagagctt
     4141 gtatttggct ccatggttag tgaaagaaga taagctcgaa aaaggcaaat taataaaaga
     4201 caataaatat atttataaag aaatagtaag agatggagga gtgttttggg acttttcaag
     4261 tatcactgag aacggagcac ttggagatgc aacaaaatca agtattgaat tgggtaagga
     4321 aataacagat ttggtaattg atagattatc agatatgata gataaaataa tactttgaaa
     4381 cataatggat ttattaaatg tccataaata cagtattatt agtaatacaa gatcataaaa
     4441 aagattttgt tttgttgcat tgttttatat aggttaagga aatgctgata tagatgaatt
     4501 tttattatgt caaagactgt ctttttatga gatttcatgc aaatctcgta aaacagtctt
     4561 ttttttatag cccgaaccct aattatcata tgaaacctgc aatgaataac cgtattttta
     4621 tagtgaacac atttaggatt acatatattt ttatatatgt taaaaataaa catttcattt
     4681 tttttaaaaa taaagtaaaa tattataata atagttgaaa ttttgtcatt ttgtgttata
     4741 ttaatattat acgctatgag gtgaaacagt gattgagttt aataatgtta caaagatata
     4801 taagaaaact tttaccgctg taaaaaatgt caatttaaaa attgataaag gcgaatttat
     4861 cttcattgta ggaaaaagcg gagcaggtaa aagtacctta ataaaattat tgcttaggga
     4921 aataagtcct tcggaaggaa ttattacata taataatcag gatattaata aaatcagaaa
     4981 aagaagcgtg gcggaataca gaaggcgtat cggctttatt tttcaagact acaggctgct
     5041 tcctaagatg actgtatatg aaaatattgc atttgccatg gaaatgattt gttgtcaggc
     5101 aaaaaacatt cgaagagagg ttccactggt tttgagcatg gtgggattaa gcgataaggc
     5161 caaatattat cccgatgaat tgtcaggagg agagcagcag cgtgttgcta ttgcaagagc
     5221 tattattaat aaacctgaaa ccgtaattgc agacgagcct acaggtaatt tggatatgga
     5281 aacatctaag gaaataatga aaatattgac ggaaataaac aggcgaggaa ctacggttat
     5341 tatggcaaca cacgacagag aaatagtaaa tcagtatcaa agaagagtag ttgagcttaa
     5401 aaacggtatt gttatcagag acatcaaggc agggggatac agctatgaat ccgaagaaga
     5461 ttagctatat attcagacag gcatttaaaa gtatgtggcg aaatcgaatg atgggttttg
     5521 cttccatagg ctcagtctcg gcagtactga taattctcgg atttgtttta atactggtat
     5581 taaatataaa taatatggca atggttgcca aggaaacttt cgaccaaatt gcagtatata
     5641 taatggaagg cgtggatgat gagcaaatag aggaaatggg taactccttc agaaaaatcg
     5701 acggagtaat gggagttgca ttccaaacaa aggactatgc tcttgaaaag gttaaggaag
     5761 attggggcga agatgctatt ttactgcagg atttaagaca caatcctttt ccaaacacat
     5821 atattgtaca gctttccgat gtaagcaagg cagataaagt aattgcacaa ctgcagactt
     5881 ttgaaggtgt tgataaggta aggtattatc aggatgccgt aaacaccctt ataaagttag
     5941 ctgattttgt taaaaaattt ggagcaggca taataataat acttttatta ataagtgtat
     6001 ttattatatc caatacaatc aagattacgg ttttaagcag acaaagggaa attgaactta
     6061 tgcaatatat cggagctacc aacggatatg tcagaggacc ttttatactg gaaggtgtca
     6121 tgttgggact attgggctct gtattggcaa ttttacttat tatgcttggt tataattata
     6181 ccatcgatta tctcgccggt cgttatgttg cacttatatc gggaatgtca ggatacttgg
     6241 tcggcgttga aatggtactg tatgacctta tcataatttt tatgacaata ggaataggta
     6301 tcggagtatt aggaagcttg gtttcattga aaaaattctt gagcatttaa ggagtgtatt
     6361 atgtttagaa aatgtgtcgc tgttattctg ttaataataa caataatagg tatttttccg
     6421 gcaaatgcag gaggaagtat tgctgatcta aggtataagc agaaacaaat agagcagcaa
     6481 atcaaagaca tacgtaaaaa ggcagaagaa cttaaaggcg aaaagaaaag tattgaagct
     6541 gaaattcaaa ttcttgacag tgagctcaga gcattaactc tggaaaccga aaaacaagaa
     6601 gttcaaaagc aagagctaaa tatgcgaata gcagaatcag agcaaaaaat tgctgagctg
     6661 actgatgaaa tagataagaa taatatgctt ttggagcaac gcttaagagc tatgtatatg
     6721 aacggaacag gcggatatct ggaagtaatt ctcaatgccg aaaaccttat cgatgcatta
     6781 acaagaatgg atttgattca attaatcgtt aaaagcgatg ttgaattact aaaaactata
     6841 aatgaacaaa agaaagaagt tgaggaatta aaagctgccc aggaaatcag aaggcatgag
     6901 ctgactgcag taattaatga tttaaatatt aagcaaaatg aagtattaat agcttctcgt
     6961 tcaaaagaaa actacatgtc cagcctgcaa aataatattg aagaaattct aagacaggaa
     7021 gctgccatgg aagagcagtc aaagcaaatc gaaaaagaca ttatagcatt acagcgtgct
     7081 gttgaatatg caggaggcga attactgtgg cctctccccg gacattacag gattacatcc
     7141 gaatatggag gaaggattca tccgataaca ggggtttggt caactcatgg aggtacggat
     7201 attgcagctc catacggaac gaatattcta tcatcaaatg acggggttgt aatatatgca
     7261 ggataccatt attcttacgg caattacata attgttgacc atgggggagg aattgcaaca
     7321 ctctatgccc attgctcaaa gttgttggct tcagagggac aagcagtaac taaaggcgag
     7381 cccattgcaa aggttggctc aacaggagaa tctacgggaa atcaccttca ttacgaagta
     7441 agacttaacg gagtcaggaa aaacccgatg gaatatttaa agtaatgata gttagggacg
     7501 gttcttttca atcacacgaa aagagccgtc cttgatattc taaataagga gtaattatga
     7561 gaattggaat agtcggtttt ggaggagtag gcagagcatt tttaaattta atttataaca
     7621 aaaaaaataa attggaaagt gaaggaattt attttcaggt taattatata ataggcagaa
     7681 ccggtggagt ttataataag catggcatag atttacataa atttattaac tttcttttcg
     7741 atgaaaaaga cataaccaag tattgtgacg ggggaaaagc agaagatata aattttgagc
     7801 ttatgctcaa taataaggat atagacttaa tggttgaaat gacacctacc aataaagaga
     7861 ccggagagcc cggcatgact catatcacaa ggtgtcttga aaacaatatc aatgtagtga
     7921 catctaacaa aggacccata ctgctggctt atcataagtt atataatttg gctaaagcta
     7981 ataaggtaca actgggtatc ggttgtacaa cgggaggggc actgccttct attaacggag
     8041 gatttatcga tttggccgga gccgatatta tttctattga aggagtttta aacggaacta
     8101 cgaacttcat tttaaaagag atggaggata caggctgtaa ttatgacgat gccctgaagg
     8161 aagctcaaag gctgggtata gctgaaacaa atccggctct cgatgttgaa ggatttgata
     8221 ctgcttcaaa actgttaata ttaacaaatg tattgatgaa tacggaaaaa actatggatg
     8281 atatatttat agaaggcatt acaaaattaa caccccatga tataaataga gcaaaatcca
     8341 tgaacaaaaa atataagctg gtaggtaaaa cacaaattct tgataataaa atagaaatgg
     8401 aagtaaaact gcaattgctg gacccctcca atcctttata cggagttgag ggtaagaata
     8461 aagcagtaag atatatatcc gacacattag gggaattaac gattatgggc ggagcttcgg
     8521 gagttactcc tgcagcagct tcaatattgc gagacataat aaatattaac agaggttaca
     8581 agtttgtaaa gtagattttt agttggctgg ggacggttct ttttgttctt gaaacaaaaa
     8641 gaaccgtccc tactttcacg ccacgataat cgagtatact ctgtgtttgt catcctgagt
     8701 gtaagcgaag gatctcaaat cagagctgtt tttttggttt catccatcaa ttcgtctaat
     8761 ttttctgcgt tcatggtttc atatttcaat aagtattgag caattttatc aacaactgct
     8821 tcattttctc taattatcgc catggcgctt tcatatgctt cttttgtcat ttcttgggct
     8881 tcatccttga tattgtctaa ataatattta ataaatcttt catcaatgca tgtgttttga
     8941 gtatttttgc ccatgccgta attgcagata atttcataaa taatatttgt ggactcttta
     9001 atatcatttg aagcacccgt cgatatttca ccgataaata tttcttcgga agctctgcct
     9061 gcaagaagta caattacttt gttgcaaagc tcgtttttcg tgtagaggaa tttatcttcc
     9121 gtagggaatt ttaaaacata tcccagggct ttgtctcttg gaattattga aattttctga
     9181 acttggtcta tattaagaat tttagcagca actgcatgac ctgcctcatg atatgcaaca
     9241 attcgttttt ccttgtctgt taccgtggaa tgttttattt ccaaacccgc ggcaattttt
     9301 tcgatagcaa attcaaaatt ttcattggta attttttttg atttttcctt tattgcttta
     9361 agtgctgctt catttgctat gttggcaagc tgagctccgg aaaacccgtg agttttaatg
     9421 gcaatatcct taagctgaac ctttttgtca agaggtttgt ttttagtgtg aacttccaag
     9481 attttaagtc tggaatgaaa attagggttt ccgacataaa tttttctgtc aaatctgccg
     9541 ggcctcagca aggcttcatc caataagtcc agcctgttgg ttgcagcaat aacaattaca
     9601 ttgctggtgt tgttaaatcc gtcaagctca attaatagct ggtttagggt ttgatccttt
     9661 tcattgttgc tgtcggcatt tcttttggca ccaagagcat ccacttcatc tataaaaatt
     9721 atactgggtg cttcctttcg agctttttca aatatggttc tcaccctttt agcacctaca
     9781 cctacatatt tttctacaaa ttccgagccg ctcgaataaa tgaatgttgc ttttgcttct
     9841 cctgctattg ctttagcaat aagtgttttg ccggttcccg gaggtccgta taagagtatg
     9901 cctcttggaa cctttgcatc cattaacctg tatttatctt cattattcaa aaaatctata
     9961 atatccatta aatcttcttt aatctcctca agtcctgcca cgtcattaaa gcttatttga
    10021 attttttgct catcaatttt tttcttatca aaatatgtta cggtggaagc tatctcattt
    10081 acttccatat catcatcttg tttcttattt ttcatataat aaaacattgc tttagaaata
    10141 aacattgtat ataatagtaa cggagtaaaa tatagtagtg gtagtttaat gttttgaggt
    10201 agtgtagcaa ataaaagaat atatactata aagaaaatta ttaatgggaa atatttaatt
    10261 ttcatattac ctccttatgt cgaaaactct aaatattttt attgatatct ttatctttac
    10321 cattacaaaa aaagttatca cgtaattcta taaataattg ttgacattta ttgttatgtt
    10381 gtatatactg ttaaagtgtt caagaagaag taaccgcttc tcaccttgcg gcgttttatg
    10441 aactgccggc aaggttaaaa aataaatgca attttgtatt tttttagatg gcggtgaaaa
    10501 tcgctgtttt ttttatagcg ctgacccatt tttagaggta tggggacaca ctttgaatag
    10561 aaatataata taatatatta ttatatataa gaaacatctg aaattttagg agggatggat
    10621 tattaaggaa cttcaaatca acgagcaaat tcgtgagaag gaagtaagag ttgttgactc
    10681 tgatggtagc cagcttggca ttataagtac aaaagaggct ttaaagattg cggaaagcaa
    10741 aaagcttgac ttggtgaatg tatcgcctaa ttcagatccg ccggtgtgca ggattatgaa
    10801 ttacgggaaa tataaatatg ctcaggcaaa aaaggaaaaa gagtctaaaa agaagcaaaa
    10861 agtaataagt gtcaaagaaa tcagaatgac accaaatatt gaagagcatg acctggcagt
    10921 caaagctaag aatgcatcaa aattccttat agacggtgac agagttaaag ttgttgtgcg
    10981 tttcagagga agagaacttg gaaagatgga taggggcaga gaagtattga tgcaatttgc
    11041 tgaaatgaca tcagaatatt ctacaataga taaacaccct gttgtagaag gcaaaaatat
    11101 gtctatgttt ttgaccccga aaaaataact aaaggaggaa ccactatgcc aaaaattaaa
    11161 acaaacagat cagctgcaaa gaggtttaaa aaaaccggaa gcggtaaaat acaaagagca
    11221 aaatcaggca aaaatcattt cacagggaaa aaagcttcta aatcaatcag aaatttaagg
    11281 aaaggtacat tggtatcagg agctgacgaa aagaaagtaa ataaattaat accatattaa
    11341 tatggaacat aaggaggaac aacaatggca agagtaaaag gtgctgtaaa tgctaagaaa
    11401 aaccatagaa aaatattgaa acttgctaaa ggatattacg gagcaaagag taaattatat
    11461 aaaacagcaa accaagcagt tatgaagtca ctttcatatg cttatgtagg aagaaaatta
    11521 agaaaaagag aattcagaca gttgtggatt gcaagaatta atgctgcagc aagacaaaac
    11581 ggattatctt acagcaaatt tatcaatttg ttgaaggtaa acaatattga aataaacaga
    11641 aaaatgctgt cagaaatggc cattcacgac ccggaaggct tcacaaacct tgtggaacaa
    11701 gtaaaaatat cctaatacaa attaataccc tatcaagact gtatatacag tcttttttat
    11761 tgcaaaatag tttcatcatg ctataatgaa ttcatataag aggctactta ggagttattt
    11821 atggatatta ttacaagcaa ggataatatt aaaattaaat atataagaag tttgaatttg
    11881 aaaaaattcc gcgatgaaga aaatgcattc cttgtggagg gaataaagtt tgttgaggaa
    11941 gccataaatg aaaatgcaga tatcaaacta ttgcttgtaa gcgaggaaac acttaataaa
    12001 agtgaagtga aaaaactcat tgattccata gatgaaagca aggcagttgt atgtacagcc
    12061 caagtgttta acagtgctgc tgataccgtt aatgcacagg gagtgcttgc tgtaatcaat
    12121 aaaaataccg catttcaaaa tgatattatc gataaattca attttattat aatgtgtgat
    12181 agaattcaag accccggcaa tttaggtact attataagaa ctgccgatgc tttcggacct
    12241 gccgccttat taattaataa gggatgtgtt gatattttta atccaaaggt tgtccgtgct
    12301 tctgcaggtg caattttcag ggttcccctt attcattcaa aagatgattt agaagttata
    12361 aatattatga aagataaagg attttatata gtatcaactg ttgttgattc cgaatactat
    12421 attgacaaca ttgaaaaaag gaacaaaatt tgcatagtaa tcgggaatga aggacaggga
    12481 gtatcgcagg atattattga taattcggat atttcagtga ccataaaaat gacagggaaa
    12541 gcagaatcaa ttaatgcatc tgtagcagca ggcatatgta tatatgaaat aagaaaaaag
    12601 ttgttgaaat aaacttttct atgtagtata atcttgcgat aaggagacat acctcttcat
    12661 ataaattaaa tattaggcaa tgataaagag tagtaagtat aagtaaatgc tttttagaga
    12721 aaaatcatca taggctgcaa gtgattttag gcatttatac cgaagtttcg ctttggagcc
    12781 tgcagaatga aaattagtag ttttgcacgg gaataccgtt attcaaataa gactaatgaa
    12841 aatcaactat gttaattttc attagtgaaa aagagtggta ccgcggactt cgcctcttat
    12901 ggaggagtcc gtaatttttt tataaaggag tatttatgaa agagcaatta ttaaaattaa
    12961 aaaccgaagc attgaaccgg ttaaaggaat gcaatgacaa tgcagatatt gagaaaatcc
    13021 gtattcaata tttagggaaa aaaggtgaat taacatcaat tttgcgctcc atgggctcgc
    13081 tttccgagga agacagaccg gaaatgggaa aactggccaa tgaagtaaga gctctcatag
    13141 aagaagcaat ccaatcaacg aaggaaacag tacatgaaag ggaatcagaa aaaagactga
    13201 aaactgaatc cttggatata acaatgcccg gaagacaacc tgaaatagga agaagacatc
    13261 ctttgatgag ggttaaagaa gagttggaaa gcgtgtttat gcgaatggga tacgatgtta
    13321 tcggcggtcc cgaaattgaa acggtagcca acaactttga tgaattaaat gcaccatacg
    13381 accatccttc aagagactgg tcagacacat tttatatttc cgaagattta gtacttagaa
    13441 cacatacgtc accggtagaa ataagagtaa tgaaggaagc agtaagggaa ggtaagctcc
    13501 cgattcgtat ggtatcgaca ggcagaacat tccgatttga tgatgttgat gacacacatt
    13561 cacccatgtt tcaccaaatg gaatgtctgg ttgtagacaa aggcgttaca atggcaaacc
    13621 tcaaggactc aattgaccag tttgtcaggg aattattcgg aagcaacgtg aaaacacgtt
    13681 ttagacctca taacttccca tttactgagc caagtgcaga agtagatgta tcctgccata
    13741 tatgtatggg caagggatgt gctgcatgca acggaacagg atggagcatg gagcttttgg
    13801 gctgcggaat gactcatccg aatgttctca gaaattgcgg catcgaccct gaaatttatt
    13861 cgggatatgc attcggtatg ggaatcgata gagttgccat ggttaaatac ggaatcccaa
    13921 acatcaggtt attgtttgaa aatgatgtca gattcttaaa acaattttag gaggtttctt
    13981 atgttagtac ctattaaatg gatgaagaaa tatgtggata ttgatgttga tgataaaaca
    14041 ttggcggata aattaacact taccggctct catgtggatg cggtaatcga ttataacaag
    14101 gaagttaaaa gggttgttac gggaaaaata ctggagataa atcaacatcc taacgctgac
    14161 aaattggttg ttacgttgat tgacataaat gaaagcgagc ccgtacaaat aattaccgga
    14221 gcaaagaata taaagaagga cgatgtggtg cctgttgcct tggaaggggc agaacttcca
    14281 aacggattaa aaattaataa aactaatttc agaggacttc catcttacgg gatgatggtt
    14341 tcttatgaag aattaggatt tgaagataag gttattccaa aggaatcaag agatggaata
    14401 attattctgc cttctgatac tcccttaggt gtggatatta aggaaatttt ggaacttgac
    14461 ggaagtgctt tggaagttga agttacattc aacagacccg actgccttaa tatagtggga
    14521 atggcaagag aagctgcagc taccttggga acacaattaa aattccccga aattaaaatt
    14581 aataatgaag aaggaaatat taaggattat cttaacggag ttgaaattga agatgaagat
    14641 ttgtgctccc gtttctatgc aagagtaata aaagatatta aaataggacc atcaccttta
    14701 tggatgcaac gaattctgat ggatgcaggg atgagaccaa ttaacaacat agttgacgtt
    14761 acaaatttcg tcatgcttga gttaggtcag cctattcatg cttatgattt agaaaaactg
    14821 gaaggcagaa aacttattgc aagaagagca aaagacggtg aaaaaattgt aacgattgac
    14881 cagaatgaaa gagaattaga taacaatatg cttgtaattg cagattcaaa aaatcctgcc
    14941 tgcattgcgg gagtaatggg cggatattat tcggaaatca gcgatgatac taaaataatg
    15001 gcattggaaa ctgcaaactt taatcccaaa tccataagag aaacatccaa aaagatagga
    15061 ttgcgctctg aagcttctgc aagaaacgaa aaacccatcg ggtatgttct ttgtgaaatt
    15121 gccagcaaca gagtatgcca gttaattgaa atgataggtg caggaactgt ggttggaggt
    15181 taccttgaag cagggaaaaa tgagtatgaa cctcctgtgg taactttgag accccatcgt
    15241 gcaagttcgc ttctgggtgt tgaaataccg gtagccgata tgcttaaatt tcttaatact
    15301 ttggaaatag aatcagtata cgacggtgaa aagataactt caagaatacc ttatttcagg
    15361 acagatatcg agcaggaagc cgatttgatc gaagaagtcg gaaggcttta tgtatttgaa
    15421 aatattgaat caaaaccgtt aagaggaagc acaagagttg gaagaaaatc cgacctgcgc
    15481 atggtagagg atgaaatcaa gtcgattctt accggtatgg gattgtatga aataaccaca
    15541 tattctttca taagccccaa gacctatgac agtatatatg cagcggaaga cagccctttg
    15601 agaaattatg taagaatatt aaatccgttg ggggaagact tttcttccat gaggacaact
    15661 ctaatgccga acatgcttga agttatacaa agaaattcca acaggggtgt tgaaggagca
    15721 aaactatatg aagcaggaag catttttatt ccgagggacc agcccgttgc tgaagagccc
    15781 ttcgaaaagt taaggctttg cataggaatg tacggcagct atgatttcta tgacttgaaa
    15841 ggaattattg atgccatgct atcgagattc ggaataaagg cagcattaaa gaaagtatcc
    15901 gacaatccaa cattccatcc cggaaggact gccgcattat gcgttgatga tgatataata
    15961 ggaatttacg gagaagtatc ccctaaggtt acggataatt acggacttga ccacagggtg
    16021 tacttagcag aaattgatgt cgaaaaaatt cttcaatata aaaatacaga gtggaaatat
    16081 gagcccttgc ctaagtatcc ttcaatggta agagatattg cggtaattgt gaaggatgat
    16141 gtgcttgcag gcgatatgga agaagaaatc aggaaagtaa atccttattt aattgaaagt
    16201 gttgaattgt ttgatgtgta taaaggggag catataaagg aaggatataa aagcactgct
    16261 ttttctgtta cttacagaaa caaggagcgc accatgaaag aaaaagaagt agaaaaaatt
    16321 catgaaaaaa tcctccaggt tctttcaaac aaatttaatg ccgtcctaag atgacaaggg
    16381 gacgtgacaa ggggacgggg gtactgtcat cctgaacgca gtgaaggatc cccccctgtc
    16441 atcctgaacg cagtgaagga tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nncatcctga acgcagtgaa ggatcccccc ctgtcatcct gaacgcagtg aaggatcccc
    16621 ccctgtcatc ctgaacgcag tgaaggatct catattgtat tgatatatat aagataagta
    16681 atatgaaaaa cattagaagg agaaaaaatg aatccagttg ctttcaatat atttggaata
    16741 gatattatgt ggtatggtgt attaatatct gcaggtgtgc tcataggagt tcttatagca
    16801 ttaaaggaag ctaaaagaac gggttttagg gaagatgatt tgatagactt tctattatat
    16861 gcaatacctg caggtataat cggtgccaga gcatattatg ttatattctc ttgggattat
    16921 tacagccaaa atccatctca gataattaat ataagaagcg gcggacttgc aattcacggt
    16981 gcaatcattg caggtgtcgt aacgggaata ttgttctgta aaataagaaa aattgatgtg
    17041 ctggagattc ttgatttggt aatgcccagc gttgctctag gtcaatcaat aggaaggtgg
    17101 ggaaatttta taaatcagga agcccatgga ggtcctacag accttccttg gggtataata
    17161 gtggacgggc aaaaagttca tcccacattc ctctatgagt caatcattga tttttgcata
    17221 tttttgttct taatttggtt tagaaaaaag aaaaaagcaa gtcacggtca aatattaggt
    17281 ttatatttaa tattgtattc tgcaggaaga tattttgttg aaggtttaag aacagacagt
    17341 cttatgttta tgggaatgag agttgctcag ctcataagca ttgcatcaat agttatcgga
    17401 gcaactctgc tcatatattt aagaaacaaa aaaacaatat aaaaaacaaa aagaactcca
    17461 tgattgtcat cctgagcgaa gcgaatgatc tcagtatgtc atcctgagtg aagcgaagga
    17521 tctcggtatg tcatcctgag tgaaatgaaa aatctctgtt tgtcatcctg agcgaagcga
    17581 aggatctcat cttttgaaag gagataaagt gtattatgtt tatatactga caaataaata
    17641 caataaagta ttatatgtcg gtattactaa tgatttaatt cgcagaatat atgaacataa
    17701 aaacaattta gtagagggct ttacatcaaa atataatgtg cataagttag tttattatga
    17761 cttaaccagt gatgttatgt cagctataac tagggaaaaa cagataaaag gttggactcg
    17821 agataagaaa attaaactta ttgaatctat gaatccggaa tggaaggatt tatattatga
    17881 tataatataa agatgagatt cttcgagcta aagctctcag aatgacacgg atgagattct
    17941 tcgagctaaa gctctcagaa tgacatttct atactcttcg ctaatatgaa gagttttttt
    18001 tatgcaatga ataagcttat gttatcttat gcaacagaga agagactcaa agattgtcat
    18061 cctgagcgaa gcgaaggatc tcatgagatt cttcgagcta aagctctcag aatgacacgg
    18121 atgagattct tcagctaaag ctctcagaat tacaggcaag tacttttcgc tgcgttaaaa
    18181 tgacaagtat ttcactcaaa aaaatgacaa aaaaaaattt tttgtatttt ttaaaaataa
    18241 ttgtggcatt ttatacccac tatgtggtat aatacaccca gtaggtggta gaaaatgttt
    18301 actggtgaat atatacattc atttgatgaa aaaggaaggg ttataattcc ttcaaaattt
    18361 agaaatgaac taggagaaac attttatatt ggaaaaggtt tggataaatg cttattcgtt
    18421 tatcctattg aaacatggac agaattcgtt ggtaagctta aaaacttatc taccttcaac
    18481 aaggaagaaa gatttttctt aagacgattt gtttcaggtt tttctgaatg ttctttcgat
    18541 aaacagggaa gaatattaat accgcctcaa cttagaagtt tcagcgaatt aaatacagat
    18601 gctgcaatta taggggttat agacagaatt gaaatttgga ataaagatac ttgggatgaa
    18661 tattccaata atgagcaatt cgattttgac agcattgccg aaaaaatgtc ggaattgggg
    18721 ataggcttat aaggaggaag catggattac atacataaac cggttttact tgacgaatcc
    18781 gtaaagggac tgaatataaa agaagacggt atttatgttg attgcactct tgggggggga
    18841 ggccatacag aagaaatctt aaaaaaggca actaagggga aagtcatagg aattgaccag
    18901 gatgattatg ctattgaaag agcgtcagag aaattaagcg gctacaaaaa ttttatgccg
    18961 gtgaaaaata atttcagcaa aatcgaagaa attttaaatg aacttagtat tgacaaaatt
    19021 gacgggataa tatttgacct tggtgtttcg tcatttcaac tggatatacc ggaaaggggt
    19081 ttttcttata attatgatat gccccttgac atgaggatgg acaaaagtca gacaaccact
    19141 gcgagacata ttgtaaacgg ttatgaagaa aatgaattga caagaattct ttgggattac
    19201 ggtgaagaaa aatgggcagc aagaattgca aaattcattg tagaggagag agagcaggaa
    19261 tttattgaaa caacggggca gcttgtatca attattaaaa aagcaatacc taaatcagcc
    19321 aggcaagaag gctcccatcc tgctaagaaa acatttcaag caataagaat tgaagttaac
    19381 cgagagttgg aaatacttga aaatacagtt aaggctgcag ttgaaagatt gaccaaaaaa
    19441 gggagaatct gcgtaataac atttcattca cttgaggata gaattataaa aaatacattt
    19501 gcggaattaa acaaggattg tatttgtcct cccgagtttc ccaaatgcat gtgcgaacac
    19561 agaagaaaac taaaaataat taccaaaaaa ccaataactc cgtcagaaga agaattaaca
    19621 gaaaacagaa gagcacacag cgcgaaatta agaataggtg agagggtgta aagaatgtca
    19681 gcactgagaa aagaagctgt tgagttacat gagcatgaat tagaaaaaga atattctcca
    19741 aaaaagcgaa taaaaagaaa taagagaaat aatactttta tagagagttt aaaaaattct
    19801 gtcatgatag caattacttt tatattagga attttaatag tctataatta tgccgtaatc
    19861 actgacaagc aaatggagct gaataatctc aatattgaaa tagaaaatct taacaacgaa
    19921 attgacaaat ataatatagc tttggagagt attaaaaaca ctaacagcat tgaagaaatg
    19981 gcaaaagttt atttaggtat gaactatccc acaaggaagc agacagtatt tgtagacttt
    20041 acatatggcc ctgatgcaat tgaagaaaag aatataatgg caaaggatga aaatctgtta
    20101 gtaggattaa ttgacaaggt aataagtttc attcgataag ggggattgcc atatgaagaa
    20161 gcgtgcaaaa agcactacta accttcaaaa taaaagaagg atgttgtttt ttttagtatg
    20221 ctttttttta gttacactag ctttggttgt aagactcggt tacatacagc tgataaaagg
    20281 tgaagaatat agaaaacaag caatggaaaa ctggtcaagg gatgttacca taagcgccaa
    20341 aaggggaact atatacgatg ctaaaggtaa aaaattagca gtcagcataa attccaacac
    20401 tgtcacatgt tttcccgcgg acgttaagaa aagcgtaaat cctaccgttg ataattcaat
    20461 agatgaagat gacagtattc ttactgcaat tttaaaaaag cttaacagag ctatgtttac
    20521 tgaggaaact caagctgaac aaatacctgt aaataccaaa acacctgaag aaatagctgc
    20581 aattctggca gaaattttag agatggatta cgatgaagtt cttgaaaaaa taacatctaa
    20641 ttataactat gtagtagtaa aaaaatggat tacagatgag caggcacaaa aaataaggga
    20701 gcaggaatta agcggcataa gtataatcga agacaataaa agggtttatc cctacggaaa
    20761 ctttgcaccc tatgtgttag gctttacgaa catagaccag gacggcttat atggagttga
    20821 agccacatac aatagttatc tgacgggaat accgggaaga acagtcgtaa ataccgatgc
    20881 ttacggaaga gagcttcctt ttggctataa tgaatattat gaatctcagg acggattagg
    20941 tgttgtgctt accatagacg aggtaataca gcattatgct gaatctgcag ccgagaaggt
    21001 tatggcagat tacaatgcaa aacgagcaac aatcatagtt atggacccta atactagcga
    21061 tattttggca atggcatcaa agcctgatta tgaccccaat gaccccagaa caccaataga
    21121 tgaaaacatt aaagccgaat gggcaaattt atctaatgaa gaacttcaaa atgcctggtt
    21181 tgacatgtgg cgaaatcctg ctgttaatga tgtatacgag cccggttcaa catttaagct
    21241 gataaccacg gcaacagctc ttgaagagaa tgttgctaca cttggctcaa cctatttctg
    21301 cgacggttat gtaagacaaa tcacaagcca taatattaaa tgttggagat attacagacc
    21361 tcacggacag caaactttat cggaggcaat acaacattca tgcaacgatg cacttgcaca
    21421 aataggtctt gatataggaa aggatgtatt ttacaaatat gtaaaatcat tcgggtttga
    21481 agagcaatcg ggtattatgc tcaatggaga agcaacaggt attgtaaaga agccggaata
    21541 tatgaaggat gtggatgttg tgacacaggc tttcggccac ggtgttgcaa taactcccat
    21601 acaattgatt aatgccgttt cggcaatttc aaacggcgga aatttaatgg tgccgagaat
    21661 tgtaaagcag cttattgacg aagacggaaa tattataaaa aatttcgata cagaagtaag
    21721 aagaaaagta atatctgaag aaacatcaaa aattatgctt caaattatga aagattccgc
    21781 agaagcagga actaagcaag cttacagacc gggatacaga ataggcggta aatcgggaac
    21841 agctcaaaaa gtaatagacg gtaaatatat tgaaggcaag tttatatctt cgtttatagg
    21901 tgtggctccc gtagataatc ccagagccgt ggcgcttgtt atggtcgatg agcccgaaat
    21961 gagcatcggg tattacggaa gtatagtggc aggaccggtc gctgcagatt tactggagaa
    22021 tatattaaaa tattatgatg tagagccggt ttataccgaa gaagagcttg gccatgtgga
    22081 aaaacaaatg gtgacggttc cgaatctttt gggacttacg gttgcggaag caacagataa
    22141 attaataagc gcaggtttgg aaagcaacat tacaattgaa gtggatgctg aaagaacggt
    22201 aaaatctcaa ttcccgaaag ctggagagca ggttgttaaa tcttccatgg ttacactgac
    22261 actaaattaa tgggacattg gggaaggtcc tcagcgtccc gccaaagtga caaaaaggac
    22321 cgtccccagt gtcacaagaa tcaattgcgc agtttaatta attatgataa aattgcacat
    22381 actatcatac atttaaatag gagtatactt atgaatataa aaaatctgct caaagaaatg
    22441 gattatttaa gctatatagg cgaagacgat atagaaataa ggggcattac aaacgattca
    22501 agaaaagtta acagaaatga tatatttgtg gcaattaaag gatttacaaa cgatggtcac
    22561 aaatttattg aaaatgcttt ggaaaatgga gcatctgcgg ttatatgcga aaatattcct
    22621 gaaaatgtaa aaggtaaaag caattttatc attgttaaat cagcgagagc atcaatggct
    22681 aaagcagcca acataatata cggacgacct tccgaaaaaa tgaaaattgc cggagtaacc
    22741 ggaactaacg gcaaaaccag cactacatat ttattaaatg gaatatatga ttatttagga
    22801 gtgaaatcag gaataatagg aacgatgggt gttcttattg aaggtaaaaa aatcaaagta
    22861 gacaacacta ctcctgaagc ttttgatata cagcactatt tggcaatgat gcttgaaaag
    22921 gatattacac attgttgtat ggaggtttca tctcatgctc ttgaattgaa cagaattgac
    22981 gaggttaaga tagatgtggg tattttcacc aatgttacaa gagaccattt ggattttcat
    23041 aaaacaatgg aaaactatta tcaagcaaag aaaaagctgt tttatttaac aaaagttaac
    23101 aatattataa atgtggatga cccttatggc ttgagacttt acaatgaact tataaaagaa
    23161 ggtataaaag caatatccgt aggtattgaa aatgatgcgg atattaaagc ttcaaacttg
    23221 aaaaccacga tgaatggaac aacttttaac cttaatgtaa tggggaaaga gaaaacagcc
    23281 catgtaaata ctcccggaac gttcagtgtt cttaattccc ttggagcttt gggagcagca
    23341 tatgcactgg gtggaaatat tgatgatata attgaagctt taaaacaaat aaagggagtt
    23401 accggaaggt ttgaaatcct ccctaacaag cttgattgta ctataatact tgattttgcc
    23461 catactcccg acggtcttca gaaggtaatg gaaactataa atgagtttgc agaaggaaga
    23521 aaaattgtta tgtttggagc cggcggagaa agagatttgt caagacgtgc cccaatgggt
    23581 gaaattgcag gcaagtactg tgatttaagc atacttacat ccgacaatcc cagatttgaa
    23641 gacccttacg aaatatgtaa ggaaatagcg gaaggagtta aaaagtcaaa cggtgaatat
    23701 aaaataatag ttgagcgcga ccaagccata tactatgcta ttgacaagag ccaaaaaaaa
    23761 gatgtaatat tgttagcggg caaatccaca gagccttatc aggatatggg aaccgaaaaa
    23821 gtaccttatg atgaaactat gcttgctaaa agaacaataa aagaagtgga gaaaaaacga
    23881 ggattgtcat cctgagcgat agctgccttt ttgtcatcct gagcgcagcg aaggatctca
    23941 gggtttataa tatgagattc ttcgagctaa agctctcaga atgacacgta gctttttcat
    24001 agcaatgaca attgatataa ccaatgaatt aggagttatt atgcaaggta acggacaaat
    24061 aataagagta ataatagtat cctttttaat agcattgttc ttaggacccg ttgtaattcc
    24121 ggtcttaaaa cgccttaaag taggacaaag tatacgagag gacggacctc aaagccattt
    24181 agccaagtcg ggtaccccta caatgggcgg aattattata atattagcaa ttttaacttc
    24241 aattataacc ggaaggctca ttacaaaaga agtactggct gtaatattct tcatgctggg
    24301 ttttgggtta ataggagcca tagatgatta taaaaaggtg gtaatgagac aaaatgaagg
    24361 attaaaaccg tatcaaaaaa tcgtgggaca gctgattttt tccgtcctgc ttgcttggta
    24421 cggctcaaga tacagcgcca tgaaaactgc attgatgatt ccattcgtta atatgcacat
    24481 tgatttaggt gttttgtaca tacccttcat tacatttatt gccgtaggta ctgtcaatgc
    24541 cgtgaatttg accgatggac ttgacggtct taacaccgga gtaacattga tagttatggc
    24601 cgctttcagt ttgatagcaa acagtttaaa agaaacaaat caaatatatg aaggaatatc
    24661 attgatatgt gccgcaacaa gcggtgcctg ccttggattt ttaaaacata acgcaaatcc
    24721 tgccaaagta tttatgggag atataggctc attggctttg ggaggtgttg tttcatcagt
    24781 tgccgtttta tccggcatgg ttttatttat acccataatt ggcggcatat actttgttga
    24841 agcactttca gttattattc aggttttcca ttataagaga acaagaagaa gagtatttaa
    24901 aatggcacct ttgcaccatc atttcgaaat gtgcgggtgg aaggaaacaa aggtagtgtc
    24961 ggccttttgg attgctactc tgatattggc atttataggt atttattcaa tataaacaac
    25021 ataagataat aatgggcatt ataaggaagg taaaaaatga atgtacttgt ggtaggatta
    25081 ggagtttcgg gcattgcctg cataaaagga ttaagtaaga caggtgcgaa tatttatgca
    25141 tttgatgatt caaacgagga gaaattaaga gacagattaa aagaagtaga aggtattgaa
    25201 gttgaatact tcttcggcaa tgaagaaata aataaaataa aattcgggaa tttggattat
    25261 gccataaaaa gccccggtat caaatatgaa acaccgatta tcgaaaaact tgttaaaaac
    25321 aatattgaag taataagtga catagaagcg gcttacaggg tgacaaattc acccatcgtt
    25381 tccataacgg gaactaacgg caaaacaaca accacaactc taattggcga aatggttaga
    25441 gaaagcaaaa aaacctataa ggtaacggga aatataggtt ttggaatata ttacgatgca
    25501 ttaacggcaa aggaagaaga aattcttgtg gctgaggtaa gttcttttca gttagcaggt
    25561 acttcaaagt acaaacctca tataagcgta ataaccaata ttactcctga ccatttagac
    25621 tatcatcaca cggtagaaaa ttatatagaa gcgaaattta aaaatgtaat taatcaagat
    25681 gaaaatgatt atgcaatatt aaattatgaa gacgaaacaa tcaggaatca ttctgataaa
    25741 ataaaggcaa aaaaaatatt cttcagctcc gaaagaattt tggaggatgg aatatattca
    25801 gaaaacggtc aaatgtttta cagaaccggt aacaataaac tatccattat caatacaaag
    25861 gatatattta taccggggaa acacaattta gaaaatgcaa tggctgccgg cggtgctgca
    25921 atagctcttg gaattgatat tgaagttata tcaagagttt taagagaatt taaaggtgtt
    25981 gagcatcgtc ttgaatttac cggcgaatac aagaaggtta aattctataa tgattcaaaa
    26041 ggaacaaatc ccgatgcatc aattaaggca attcaaggaa tcgaaaaacc gattatcttg
    26101 atagcaggcg gatatgataa gaagtcatcc tacgatgatt ttattaaggc atttgacaat
    26161 aaggtaaaag cattgatact tcttgggcag actgccgaag atattcaaaa gtgtgctttg
    26221 aaatatggtt ttgaaaatat ttacagagtt gaaacaatgg atgaagccgt gaagaaaagc
    26281 tttgaattgg ccgaagaagg agacaatgtt gttttatccc cggcatgcgc cagctggggg
    26341 atgtacccta attatgaagt cagaggcaga gattttaaag aaagggtaga atattatgga
    26401 agaaaccgta agaaaagcta aagccaggtc aattgattgg gtattggttt ttataatagt
    26461 attattggtc ttgtttggat ttttaatggt ttacagttcc tcttatcccg acggatatta
    26521 taaatatggg ggagatcctt tttatttttt acgaaaacaa gttattgcag caggagcagg
    26581 acttgccgga atgatatttt ttgcaaatct tccatacata atctacaaaa aattcagtaa
    26641 gctgatatta ttcacatgta taggattagc aatgcttacc attataatag gaattgcatc
    26701 aaatggcgcc cagaggtgga tagagattgg cggaatatca ttgcagccct cggaaataat
    26761 taaaatcgga gctgtacttt atatggctga ttatttcaac agaaaaagaa gtgaattgcc
    26821 atcctttacc aaaggttttt taccgtcgct gttttatata gtgctatttt gcgggttaat
    26881 tgctatacag gatgatttaa gcacagctgt aatattagcc ggaactctga tggttatgtt
    26941 tttttgtgca ggagcaagaa tcagccattt aatactgttg tttcttcttg cactcgcggg
    27001 cataatcgca ctgattgcaa tgcagacata tcgaatatca cgaataatag tcttttttga
    27061 cccttttaaa tatcccttgg atgaaggtta tcaaataata aattcactgt atgccttggg
    27121 taccgggggt ttatttggta tgggagtagg aaaaagcaga caaaagtttt ttcatttgcc
    27181 ggaagcatac aatgacttta ttttctcaat aataggagaa gagctgggct ttgtcggagc
    27241 ggtctttgta atgctgggat ttgtggtatt tgcatggaga ggacttagga tttcaatgaa
    27301 cacagaagat tttttcggaa gccagacagc tttgggcttg actgtaatag tactggttca
    27361 gtttttaatt catgtagcgg ttgcaacatc ttcgatgcca ccaacgggaa gagcccttcc
    27421 ctttataagc gcaggtggct catcgataat gtttttgctc tgctccatgg ggattttgct
    27481 taatatatcg aaatattcca atacttacag gtcatagtta tgtcatcctg agcgaagcgc
    27541 aatatgtcat cctgagcgaa gcgaaggatc tcataagatt cttcgagcta aagctctcag
    27601 aatgacacgg atgagattct tcgagctaaa gctctcagaa tgacacggat aagattcttc
    27661 gagctaaa
//
LOCUS       DAOPUE010000015        27198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1750_length_27198_cov_10.393582, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000015 DAOPUE010000000
VERSION     DAOPUE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 27198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27198
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1750_length_27198_cov_10.393582"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(471..1949)
                     /gene="spoIVA"
                     /locus_tag="PKH42_02660"
     CDS             complement(471..1949)
                     /gene="spoIVA"
                     /locus_tag="PKH42_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOA19361.1"
                     /translation="MTNIDIYEDIAKRTKGEIYLGVVGPVRTGKSTFIRKFSENVMLP
                     NIEDEYTRERANDEMPQSGTGKTVMTTEPKFVPSDAVMVTFSNNVRAKLRLIDCVGYM
                     IPGAIGNMEGEVPRLVSTPWNDEPIPFAEAAEIGTKKVINDHSTIGVLVTTDGSITDI
                     NRDNYIMAEERVVRELKEINKPFVIILNTTHPKNENTVALSYQLKEKYDAPVLIVDCM
                     NLKTDDIENVLENVLYEFPIKEINVELPRWFDGLSYDYPIKRNLINILKDDFENYYKL
                     SDFKAEDIFSEENDYVEDYYVKDIELGTGKINLAVDLDNSYYYKIISDLCGEEITGEH
                     QILKLIGDLSKTRKSYSRVEAAINDAKANGYGYVTPTVSDMIIEPPEVFKEGGRYGIK
                     IKAQAPSLHIFSAGVNTEVSPVIGSKTQSEDLMCYLTEQMKENPQSIWQAEMFGKPLS
                     SLVEEQLDSKLTAMPDNARRKLRKTIEKIVNDGQGSIIFLIV"
     gene            complement(2127..3125)
                     /locus_tag="PKH42_02665"
     CDS             complement(2127..3125)
                     /locus_tag="PKH42_02665"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOA19362.1"
                     /translation="MNKKITVLGGGSWGTALSKLLSENGHNVTVWLRDEAQAKELTVK
                     RVNEKYLPKVKIPENIVFASDINKAVEEAEILLLVIPTQMVRGVLKQIKDEYKDGKII
                     INASKGIEKGTMKLVSTIVKEETKNTIFAVLSGPSHAEEVGLSLPTAITVACENKDVA
                     EEIQDIFMSEYFRVYTNEDVLGAELGGALKNIIALGAGISDGVGYGDNAKAALMNRGI
                     VEIARLGLAMGADLHTFYGLTGIGDLIVTCTSKHSRNWNAGYLIGQGLTKDEAIKKVG
                     MIVEGIPTTYAAYELSKKLMVEMPIVDAMYDVLENKADVKDTVNKLMLRDKKEERL"
     gene            complement(3135..3716)
                     /gene="plsY"
                     /locus_tag="PKH42_02670"
     CDS             complement(3135..3716)
                     /gene="plsY"
                     /locus_tag="PKH42_02670"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOA19363.1"
                     /translation="MKYIFIAIISYFIGNISFAYILGKILTNKDVREFGSGNAGTTNA
                     IRAFGKKVGAMVFSGDVLKGVVAVLFGRYFAGQTGAYIAGAFAIIGHNWPVLLNFKGG
                     KGVATTIGVMVFVNFYLTLICVIIGVIIIIFTRTVSLGSISGMAVAPLVAIILIRPLT
                     FDLLLFTIFVASMSIYRHKDNIKRLIKGNENKF"
     gene            complement(3761..5080)
                     /gene="der"
                     /locus_tag="PKH42_02675"
     CDS             complement(3761..5080)
                     /gene="der"
                     /locus_tag="PKH42_02675"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOA19364.1"
                     /translation="MGRPIVTIVGRPNVGKSTLFNRIAGKRISITEDTPGVTRDRIYA
                     ECEWLDNYFTLIDTGGIEPNTTDTIFAQMRIQAEVAIEMADVVLFMVDGKEGVTAADT
                     EVAQMLRKAKKEIILVVNKMDRFVNEDSVYEFYNLGLGDPIPISSAEALGIGDLLDEI
                     ISKFNGKMDTKEEEDSIKIAVIGKPNAGKSSIINRIIGEERVIVSDIPGTTRDAIDTK
                     FERNSKKYTLIDTAGIRKKSKVLESVEKYSVLRAFTAVERADICVLVIDADKGVTEQD
                     IRIAGYSHDSNKGMVIVINKWDLIEKDNSTFKEFTEEIRQKLAYATYAPILTVSALTG
                     QRVNKILEAVDEIYSFRNLRISTGVLNDILTEAVLMNQPATVKGKRLKIYYGTQVAVN
                     PPKFLIFVNDKEIVHFSYGRYIENKIREAFEYKGTPIIIEFRNRSEK"
     gene            complement(5094..6389)
                     /locus_tag="PKH42_02680"
     CDS             complement(5094..6389)
                     /locus_tag="PKH42_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOA19365.1"
                     /translation="MKNIIVKVLKNSIAEELNIEPGDELLSVNNSIIKDYIDYKYQIS
                     DNLVYFEIKKKDGEIWELEIEKDYDEDIGIVFENPLMDNIKVCSNKCIFCFIDQMPKG
                     MRKTLYVKDDDTRLSFLYGNFITLTNLSDDEFDRIIQYKISPIKVSVHTTDPQLRKYM
                     MGNDKDMDILKYLKRLIDANITVDCQIVLVRDVNDKEQLTKTINDLSSLHPGLRSVAV
                     VPVGLTKYREKLTELKPFDKESAKDVVEQVTKLQETLLRKFGTRFIFIADEFYILSKY
                     DFPPYEDYEDFDQLENGIGMCRLFKNRVEEEIKNIRYNKVLKDEVTFVTGVAAYELMV
                     ELAEKIMSRINVKINVLRIINNYFGDKITVSGLITGKDIIDQLKGKNYKNLILPRNMI
                     NDNHVMLDDMVIEDLERELNTKVEIYDAEDDTLLSLIVK"
     gene            6603..8297
                     /locus_tag="PKH42_02685"
     CDS             6603..8297
                     /locus_tag="PKH42_02685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOA19366.1"
                     /translation="MKFNEYEYIRPDYNIIKEKYLNLINDFKNSATADEQYTILKKIN
                     ALANNVDTMSQLVYIRNSINSADEFYDTEKDYMDMTSPKFYGLRIEFYKQLLNSRFKE
                     ELTDKIHPQLITLAEMEIKTFSDEAIPLMQEENKLISEYDKLISSAKIEYDGKLLNLS
                     QMQPYMESKDRRIRKEAHEKSSNFFKENEAKFDLIYDKLVKIRHQIALKLGFNNFIEL
                     AYLRNGRSDYNSTDVRNFREHVLKYIVPITNELRERQAKRIGVDEFKYYDIPLNFISG
                     NPTPKGNKDWIMERTKKMYEELSPETHEFINIMIDRELFDLETKPNKQAGGYCTFLHD
                     YKSPFIFANFNGTQDDVTVVTHEAGHAFQTYQSRHFDAPEYSFPTSEAAEIHSMSMEF
                     ITWPWMELFFEKDIEKFKFFHLADSLCFIPYGVTVDEFQHIVYEYPDMTPDERKKAWR
                     DIEKKYTPYKNYDDNEFLNKGTFWFKQGHIFSSPFYYIDYTLAQICSYQFWLRFNENR
                     KEAWEDYLKICKIGGSQSFLEILKAGNLKSPFEESTINSVASKIKEYLNTINDMKL"
     gene            complement(8372..8542)
                     /locus_tag="PKH42_02690"
     CDS             complement(8372..8542)
                     /locus_tag="PKH42_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA19367.1"
                     /translation="MAYIITDACISCGACEPECPVGAISAGDDKYVIDAATCIECGAC
                     ASVCPVEAPQQD"
     gene            complement(8654..9544)
                     /locus_tag="PKH42_02695"
     CDS             complement(8654..9544)
                     /locus_tag="PKH42_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOA19368.1"
                     /translation="MIKVVGVRFKKAGKIYYFDPEKKWINNGDFVIVETIRGIEFGQV
                     VVGPKMVKEEDIVQPLKKVLRLATEDDIKRNRENKEKEKEAYDICFKKIEEHGLEMKL
                     IDIEYTFDNNKVIFYFTADGRIDFRELVKDLAAIFRTRIELRQIGVRDEAKMIGGLGP
                     CGRHMCCSSFLGEFYPVSIKMAKEQKLSLNPAKISGICSRLMCCLNYEHQVYEENIKK
                     LPDIGDKVQVLGTNKTGIVIDINPLFVTAKVNVEKSDGTIEVEEIQGSRLKVVEEGVL
                     KHYDAEDTDIDIEELKELED"
     gene            complement(9541..10512)
                     /gene="holB"
                     /locus_tag="PKH42_02700"
     CDS             complement(9541..10512)
                     /gene="holB"
                     /locus_tag="PKH42_02700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOA19369.1"
                     /translation="MLYSQVAGQEEIKTSLIKSIDNNQVSHCYLFEGPEGMGKFELAL
                     VFAQSLLCLNFHKEPCNKCKNCIKVNSLNHPDLHIINKDGNSIKREDIDLLINTVYNK
                     PYEAKRKIFIIKDAHLMTPQAANGFLKTLEEPPGDTVIILLTINSNLLLPTIVSRCQE
                     IKFKNISKDSIKSYLKDYDSDRVELAANYSKGILSKAINIIKGSDDTLKKREEIIKIF
                     DKIVNSDSEIIFELESYFDDKKDDINSIIEIMIMWIRDIIFVNNNMDRLVINKDYINL
                     LKTHGKNMKKDTDIIEYMQNTSDNIKSNVNYKLTVDNMLLKIQEVFK"
     gene            complement(10523..10855)
                     /locus_tag="PKH42_02705"
     CDS             complement(10523..10855)
                     /locus_tag="PKH42_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOA19370.1"
                     /translation="MKLITAVVQNDDANKLISALRENGISSTKMSSTGGFLSSGNTTV
                     ISGIEDDRVDEAIEIIRKICKTKKKVTAAIPPSSFSTVGGYLPQTIEVTVGGAVVFVT
                     PVDRFEKI"
     gene            complement(10866..11474)
                     /gene="tmk"
                     /locus_tag="PKH42_02710"
     CDS             complement(10866..11474)
                     /gene="tmk"
                     /locus_tag="PKH42_02710"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOA19371.1"
                     /translation="MEGRFIVLEGSDGSGKSTIAERIGRYYSEKGRDIEFTREPGGTS
                     ISEKIRELILDRDNTEMDYRTEALLYAASRAQLVSEKIIPWLKAGKVVISERYVYSSL
                     VYQGLGRGLGIDEIKKINDFATAGIKPDLVMLLDVEPEKALKRKLCIDGGDRLENEDI
                     SFHRSVYEGYKKLSSHFPEIITIDAERSIEEIFEEIKGLLTI"
     gene            complement(11456..12901)
                     /locus_tag="PKH42_02715"
     CDS             complement(11456..12901)
                     /locus_tag="PKH42_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA19372.1"
                     /translation="MKKLNIISGIKQYRSEVDTNFHMPGHKGKPEILGELEKNLNFFD
                     ITETLGTDNLHYPTGFLKDAMNYISKVYGTKKSYMVVNGTSCGIISAIMACTNPGDKI
                     LVQRDCHKSVYNACILGDLKLFYLYPDFNKKYGLNFSISLDKLDQMLTDNPDIKMVVL
                     TYPTFYGICFDVKKAAEIVHKHGKILMIDEAHGSHLQFCKELLPPPAENSGADIVAQS
                     THKTLPCLTQGSILHICSDRVNVNNIETMLRMLQTTSPSYILMASIENAIHWMAENGE
                     ERLKRNIEVFKRRTLELRNMGINVLEDDFLISEGLYDFDATRAVISMSELGITGTELQ
                     DILRYKYKIQMEFADLQYTVGYVTATDEPEDIDRLFDAVKEIYIEESKSKEKREIVQI
                     EPFPKLEHERKMRKAFYAENMLLHMDDAIGKTAAEFIIPYPPGIPLVCPGEIIVQDTI
                     DYVKIMLENGINVNGVNKNNQVRVVKWKEDL"
     gene            complement(13162..13431)
                     /locus_tag="PKH42_02720"
     CDS             complement(13162..13431)
                     /locus_tag="PKH42_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOA19373.1"
                     /translation="MTGADLGIILAYTAGIMLVFMTSWIFFKPLKFIGKIILNSVIGA
                     IVLIIFNYFGQYTGVHIGVNEFTALIIGILGVPGFFAILLVKLLI"
     gene            complement(13532..14056)
                     /locus_tag="PKH42_02725"
     CDS             complement(13532..14056)
                     /locus_tag="PKH42_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOA19374.1"
                     /translation="MIKIGMRNIKTTISVFICLLVFELINRNNPIFACIAAVICVQNT
                     IVDSKEIGFSRIIGTIIGGLAGAFTLFIVNTFFNHKILIFIIPLGIMLLIQICVTINM
                     KKAVVICCVVFLVITLNHDEGYILYTFNRVLDTSIGIIIALFVNKYVKIPEKLKSKIG
                     DKEELIETHENSEP"
     gene            14307..15497
                     /locus_tag="PKH42_02730"
     CDS             14307..15497
                     /locus_tag="PKH42_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOA19375.1"
                     /translation="MERIVGTVVRGLRAPIITKGDDIVKITVETVLKASKTEGFSLRD
                     KDIVGITEAIVARAQGNFANIEQIALDVKQKFDSDTVGLIFPILSRNRFAVCLKGIAK
                     SFKKIYLMFSYPSDEVGNPLVDYDLLDKEGVNPWTDILKENEFRSHFKNTKHIFTGVD
                     YIKYYSEIIRESGCEVEVIFANNPISILNYTKNVLTCDIHTRNRSKRLLKSNGAETVY
                     GLDDILNQSVEGSGFNEKYGLLGTNKATEDTVKLFPRDCQSLVEKIQEKLFELTGKKI
                     EVMIYGDGAFKDPVGKIWELADPVVSPGYTEGLIGTPNEFKLKYLADNQFPHLKGDEL
                     KKAICDYIMNKDCDLKDRMESQGTTPRRLTDLIGSLCDLTSGSGDKGTPIVYIQGYFD
                     NYSV"
     gene            complement(15599..17056)
                     /locus_tag="PKH42_02735"
     CDS             complement(15599..17056)
                     /locus_tag="PKH42_02735"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOA19376.1"
                     /translation="MYYNNFGERRLSMLMDFYELTMANGYFSNGMKDEIVYFDLFFRK
                     NPDGAGFSIVAGLEQIIQYIKELKFYEEDINFLRSKKLFTEEFLQYLKDFKFSGDIYA
                     IPEGTPVFPNEPLITVRAKTIDAQIIETMVLLTINHQSLIATKANRVVRAAKGKPIME
                     FGARRSQGYDGAIYGARAAYIGGVIGTATTLADEAFGVPALGTMAHSWVQMFESEYEA
                     FKSYAQNYPDACNLLVDTYNVVKSGIPNAIRISKEVLEPMGKRLKGVRIDSGDIAYLS
                     KKCRKMLNEAGLHDCAIVASNSLDEFIISDLLDQGAKIDYFGVGERLITAKSEPVFGC
                     VYKLVAVERDGKIIPKIKISENEEKITNPGYKKVWRLYDKKTNSPIADVITLADEIID
                     NSKPYTIFDEIHTWKKKKVTSFYAKNLQVPVFINGECVYESPSLNEIREYCKKKVGNL
                     WGEIKRFSNPHHYYVDLSKELWDCKQHLISQYSFK"
     gene            complement(17198..18007)
                     /locus_tag="PKH42_02740"
     CDS             complement(17198..18007)
                     /locus_tag="PKH42_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOA19377.1"
                     /translation="MVIVKAVAVTGGIGAICGLLLALAAKLFHIDVDQRITDVRNALP
                     GANCGACGYAGCDAMAEAIAAGRAPVDGCPVANYQAHAKIADIMGTSAEEGEKKVAHV
                     LCRGNDCLASKKHEYHGVRDCKAAAAVQGGDKSCDKGCLGYGNCVRVCDFDAIDIIDG
                     IAVIDKEKCTACGKCIEECPKAVIEFVPYKNNVIVGCNNRDFGKAVKDVCKIGCIACK
                     ICEKNCPYDAIHVTNNLAKVDYDKCTHCMICVEKCPTKVIEGDLSIKDKAV"
     gene            complement(18019..18594)
                     /gene="rsxA"
                     /locus_tag="PKH42_02745"
     CDS             complement(18019..18594)
                     /gene="rsxA"
                     /locus_tag="PKH42_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOA19378.1"
                     /translation="MELVSLFITSFIVNNIIFMQFLGTCPFLGVSKKIDTAAGMGIAV
                     IFVITLASVITYIIHYAILVNLELEYLQTIVFILIIAALVQFVEMFLKKSAPGLYQAL
                     GIFLPLITTNCAVLGVAINNIIYEYNFVESLVYSVGISAGFLLAIVLFAGVRDKVDYA
                     DLPESLKGSAISLITAGLMSVAFLGFSGLQL"
     gene            complement(18605..19201)
                     /locus_tag="PKH42_02750"
     CDS             complement(18605..19201)
                     /locus_tag="PKH42_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOA19379.1"
                     /translation="MNKLEIFKNGVTTENPILVQQVGMCATLAITTSLINGIGMGVAV
                     IAVLTGSNIVISLLRKFIPDEVRIPSFIIVIAAFTTIIDLLMHAYTFELYKALGVFIP
                     LIVVNCIILARAEAFASKNSLSDSIIDGLGMGLGYLVAMILLSSVREILGNGTILGFR
                     ILPEAYEPMMMAIQPPGAFILLGLLIGIVNYLSRRKKA"
     gene            complement(19211..19783)
                     /locus_tag="PKH42_02755"
     CDS             complement(19211..19783)
                     /locus_tag="PKH42_02755"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA19380.1"
                     /translation="MKNNFVKLAGVLLIIGAVSAGVLAWLNDVTSDRIAQNEAMASMD
                     PAILEAVMPGSVMFNDYEDTALVDTIKADNNKFINLLTAVDESGNELGKVIRTWSTVK
                     GFNGDLELYVGFTPDGQISGTSVISHAETEGVGSKVTLLAFTEQFIGKDASGEISEGD
                     YDAVSGATYSSKSFLSAVNNAINVYAEYVK"
     gene            complement(19773..20723)
                     /locus_tag="PKH42_02760"
     CDS             complement(19773..20723)
                     /locus_tag="PKH42_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOA19381.1"
                     /translation="MEIKLIGSSSPHIRSDETTQKLMLDVIIALIPALAASIYYFGYK
                     AMILVLASVISAVATEYFCQKFMNRPITVTDLSAVVTGILLAFNLPAEAPWWIAVIGS
                     VFAIGIVKQIFGGIGFNFLNPALGARAFLMASWPHHLSGGFIDPAIDAVTSATPLSII
                     KGAASGQLPSLWDMLIGNIPGVIGETSAILLLAGGIYLIYTGTIKWIIPAFYIGTVAA
                     IALLFDAGNLSYHILGGGLMLGAFFMATDYATSPITDKGKVIYAVGAGMLTMFIRKMG
                     GYPEGVSYSILLMNILTPMIDKYVKPRVFGVATGGKKNEK"
     gene            complement(20736..22067)
                     /gene="rsxC"
                     /locus_tag="PKH42_02765"
     CDS             complement(20736..22067)
                     /gene="rsxC"
                     /locus_tag="PKH42_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOA19382.1"
                     /translation="MSTKLFTFKGGIHPPQSKSFTQNVPIEDAPAPDIVVIPLSQHIG
                     APCDPLVAVGDKVKVGQKIGESKAFVSAPVHSSVSGTVKKIENRQVPGGAKVNCVIIE
                     SDGKYEVHESVQPKGKLEDLSKEDILSIIKEAGMAGMGGATFPTHVKLSPPKDKTIDT
                     LLVNGSECEPYLTADHRIMLEKPDLVLLGTKAVMKALGVNKAYICIEDNKPDAIETLQ
                     QAVGNEEGIEVVSMIAKYPQGDEKRLINAVTGRVVPSGGLPMEVGCVVENVGTVATIG
                     NVIKTGMPCIQRVVTVTGSAVENPKNLYVRIGTLFKDCIEMCGGYKEEPGKIISGGPM
                     MGIAQYTDEVPVIKGTSGILVLNKKEANVPEHSNCILCGKCVEACPVYLMPYKIARFS
                     LLRNFDGADEYHATDCIECGACSYVCPAKRPLKESISVAKKEILTRRKKAK"
     gene            complement(22224..22775)
                     /locus_tag="PKH42_02770"
     CDS             complement(22224..22775)
                     /locus_tag="PKH42_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19383.1"
                     /translation="MKEISMHILDIAMNSIKADASLIEINIEDSIKNNRLSITIADNG
                     RGMSEDVINRVSNPFYTTRTTRKVGLGIPMLKEACERCNGYLKIESKIGVGTKVCCYF
                     ERDNIDRAPLGNMGETIMTIINSGDNFELTYSHKTDRGEFIFDTREVKEMLEGININD
                     IGVLMWIKEYINENVNLVSAGKI"
     gene            complement(22917..23606)
                     /locus_tag="PKH42_02775"
     CDS             complement(22917..23606)
                     /locus_tag="PKH42_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOA19384.1"
                     /translation="MKFYYDLHIHSDLSPCSACDMTPNNIINMSLIKGLDIISVTDHN
                     SAGNLRAVMELGNKTGIKVVPGLEVTTKEEVHVLCYFKELDNAIEFGNIIYNSLPFIK
                     NNPEIFGEQNIYNSKDEITGTLEKLLINASQYTIEDIFSIVKKYDGVMIPAHINKMTN
                     GILGVLGFMPINLKIETVEVYSKMPINSKIAEKYKVIYNSDAHQLVDIAEAVNYFDLD
                     DIEEIYEYLFE"
     gene            complement(23603..23935)
                     /locus_tag="PKH42_02780"
     CDS             complement(23603..23935)
                     /locus_tag="PKH42_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOA19385.1"
                     /translation="MNCLEFAEKLQLTPLCEKEGDIEIEGVYIGDLLSIVMAKAKKNY
                     VWITIQTHINIVAIAELLELSCIIVVENMAVDNDTLDKAKELNIPIFKTSESAYQVAC
                     RLSDMGIK"
     gene            complement(23948..25189)
                     /locus_tag="PKH42_02785"
     CDS             complement(23948..25189)
                     /locus_tag="PKH42_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOA19386.1"
                     /translation="MNFSIMFDKDKCRGCTNCMKRCPTQAIRLIEGKAFINTAKCIHC
                     GECIKICPYNAYSPESIEWYNQMHDTPKKFKIAIPTTSLYGQFPRGTDICKVQNAILK
                     LGFDFVYDASWAADLVAKAIKLKIDKEKTMRPYISTYCPAAVRLIKYRYPSLMDNLIL
                     IREPMEIAGILARERVRKKFGLKDEDIKVFYISPCPAKLLAVTNPIGDYKPSVDWVIP
                     LNKIYGDLYREVIREDNITCSYPSLLGMKWAVAGGQSEHAELNNYIAVHGMENIIEIL
                     EEIENGKLSDIDFVEASACVNGCVGGTFNVVNPFIASSSINYISNSLPDECLNPDIDR
                     FDEMYKTGFFNYKLKEEEKFDKLNLKEAVERNEKIREILDKLPGLDCGSCGAPTCLAH
                     AEDVYNNESEFYDCVVLRAKK"
     gene            complement(25199..25624)
                     /locus_tag="PKH42_02790"
     CDS             complement(25199..25624)
                     /locus_tag="PKH42_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19387.1"
                     /translation="MTDKFLKRYSYNITANDFMSAGKSASAIKSNLKSLGIDSNTIRK
                     ISIISYETEINIVIHSYGGKLHCDIYENKIVIISEDTGPGITDLDMAMTEGYSTATDS
                     VRELGFGAGMGLPNIKKYSDQLEINTSSTGTTIIATIYL"
     gene            complement(25617..25970)
                     /locus_tag="PKH42_02795"
     CDS             complement(25617..25970)
                     /locus_tag="PKH42_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOA19388.1"
                     /translation="MQIKEIVDIVKADVLTNYHDGNTEFKFGFASDLMSDVLAYGRSD
                     SLLITGLNNPQVIRTVEMLDINAVLFVRGKRPCEEVIELAKENGITILATDYTMFKTC
                     GLLVMNGMEGLEIND"
     gene            complement(25982..26845)
                     /locus_tag="PKH42_02800"
     CDS             complement(25982..26845)
                     /locus_tag="PKH42_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOA19389.1"
                     /translation="MEVAILKSFTYENSGGNGAGVVVLNQQIPYDKKQEIANKVGLSE
                     TAFIYKRDENDYDVSFFTPVCEVDLCGHATIAAFYYLGKIGQIKGNDETKCVYQNTKA
                     GRLKIEITFKGQDVEYVLMQQSEPRIYGELKEKKQIADSLNIDERLIGLPNYDIAPTI
                     VSTGLKDILVPVSSRKVLNSLDVDFKMIEDISYENDVVGYHVFTIDNERVYARNFAPR
                     VGINEECATGTSNGALGSLLFSKGFSNQMEVIQGESMNQKSLIFVRIDSHNDIMDVYV
                     GGKAHIENYIK"
     gene            complement(27009..27091)
                     /locus_tag="PKH42_02805"
     tRNA            complement(27009..27091)
                     /locus_tag="PKH42_02805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27056..27058),aa:Leu,seq:gag)
BASE COUNT         8155 a         5529 c         3929 g         9585 t
ORIGIN      
        1 ctgccagtct tctacgcctc tgccttttgt gacagctttg ttagtttacc ataactgcga
       61 attaaagtca ataatttttc actcattttt tctgtatttt tgacttgtct tctcagtttt
      121 ctttacaatc ttaatcaaaa aaactttcac aaaatctact gaaatttaaa tcataaatgc
      181 aatcggcttc ttggcaatca cttttttatt ttagctcact tatttacgct tgtcaatcag
      241 atttttatct atattttact gattgtcctt tatttccaat ccttaaatat taataatata
      301 ttatgcacgt ttattagatc gtttcaacat taatataata ttaatttata acacattttt
      361 attataataa aaaaatagat tttcatattt catttttcga aatagaaaat cttttatcga
      421 agaaattaag ttaggatata aaggacatat cgctgcttaa gtcaggcatc ttatactatg
      481 aggaatatta tgctcccttg accgtcgttt actatttttt caattgtctt tctaagcttc
      541 ctgcgtgcat tgtcgggcat agccgtaagc ttagaatcca gctgttcctc aacaagactt
      601 gataggggtt tgccaaacat ttcagcctgc catatacttt ggggattttc tttcatttgt
      661 tcggtaagat aacacattaa atcctcgctt tgagttttgc ttcctatgac aggtgatact
      721 tccgtattga caccggcact gaatatatgc aatgatggag cttgtgcttt aattttaatt
      781 ccgtacctgc cgccttcttt gaaaacttcc gggggctcaa taatcatgtc gcttacagtc
      841 ggtgtcacat atccgtagcc gttagcctta gcatcgttta tggcagcctc aacacggctg
      901 tagctcttcc ttgtttttga caagtcacct ataagcttta atatttgatg ctcacctgta
      961 atttcttcac cgcaaaggtc gctgattatt ttgtaataat aagaattatc caggtcaact
     1021 gctagattga tttttcctgt accaagttca atatctttta catagtaatc ttcaacataa
     1081 tcattttctt ccgaaaaaat atcctctgct ttaaagtcgc ttaatttgta ataattttcg
     1141 aaatcatctt ttagtatgtt aattaaattt ctttttattg gataatcata tgatagcccg
     1201 tcaaaccatc ttggaagttc aacattgatt tctttaatcg gaaattcata aagaacattt
     1261 tctaacacat tttcaatatc atcggttttt aaattcatac agtcgactat aagaaccgga
     1321 gcatcgtatt tctcctttaa ctgataagat aaagcaactg tattttcatt ttttggatga
     1381 gtagtattta aaataataac gaaaggctta ttaatttctt tcagttccct taccactctt
     1441 tcttctgcca ttatgtaatt atctctgttt atgtctgtga tgcttccgtc ggttgttact
     1501 aataccccaa ttgttgaatg gtcgttgatt accttttttg tacctatttc tgctgcttct
     1561 gcaaagggta tgggttcatc gttccaggga gtactaacaa gtcttggtac ttcaccttcc
     1621 atatttccaa ttgcaccggg aatcatatac cctacacagt caataagcct taattttgct
     1681 cttacattgt tgctgaatgt aaccattact gcatcactcg gtacaaattt tggctcagta
     1741 gtcataacgg tcttgcctgt accgctttga ggcatttcat catttgccct ttctcttgta
     1801 tactcatcct caatattggg cagcatcaca ttttccgaaa actttcttat aaatgttgac
     1861 ttaccggttc ttaccggtcc cacaaccccc aagtatattt cacccttggt tcttttagct
     1921 atatcttcgt atatatctat attagtgata ataatccctc cccttgcaaa tatgcactat
     1981 tgtatacaat taatttatat gccttgttca acttctttat tccaaaaaaa caaaaaaaga
     2041 atatattcta atattctcta tattcttctt ttaaattaca agagactcga ctgaagactt
     2101 gtgacaatac ccccgtcccc ttgtcactaa agtctttctt cttttttgtc tcttaacatt
     2161 aatttattta ctgtatcttt tacatcggcc ttgttttcca aaacatcata cattgcatcg
     2221 actatcggca tttctaccat tagtttctta gacaattcat atgcagcata tgtggtagga
     2281 attccttcaa caatcattcc tacctttttt atcgcttcat cctttgttaa gccttgaccg
     2341 ataaggtagc ctgcattcca gtttcttgaa tgcttgctgg tacatgtaac tatcaagtct
     2401 ccaattccgg taagcccgta aaatgtgtgc aaatccgctc ccatggcaag tccaagtctt
     2461 gcaatttcaa caatacccct gttcataagg gctgcttttg cattgtcccc gtagcctact
     2521 ccgtcggata tacccgcacc aagagctatt atatttttga gtgctcctcc taactctgca
     2581 cctaatacat cttcatttgt atatactctg aaatattcag acataaatat gtcttgaatc
     2641 tcctcggcta catctttatt ttcgcaggca acagttattg cagtaggcaa tgacaacccg
     2701 acttcctctg catgggaagg tcctgataaa accgcaaata ttgtattctt tgtttcttcc
     2761 ttaacaattg ttgaaaccaa tttcatggta cccttttcaa tacccttgga agcatttata
     2821 attattttac cgtctttata ctcatcttta atttgcttta atacacccct aaccatttgg
     2881 gttggaataa ccaacaataa aatctccgct tcctctacag ctttgtttat atcagaagca
     2941 aatactatat tttcaggtat tttaactttt ggcaagtatt tttcattaac cctttttacg
     3001 gttaattctt tagcttgagc ttcatctctc agccatacgg taacattatg cccgttttcc
     3061 gaaagaagtt tggaaagtgc ggttccccag cttcctccgc ctaggactgt tattttctta
     3121 ttcatcctcg cctcctaaaa tttgttttca tttcctttaa tcagtctttt tatattatcc
     3181 ttgtgtctgt aaatggacat acttgcaaca aatattgtaa ataataaaag atcaaaagta
     3241 agcggacgaa tcaaaataat tgccacgagt ggtgcaactg ccatgcctga aatagagccc
     3301 aaggataccg ttcttgtaaa aataataatt attacgccaa taataacaca aatcaaagtg
     3361 agataaaaat ttacaaatac cataacaccg atagttgtgg cgactccttt tcctcctttg
     3421 aaattaagaa gaacaggcca attgtgacct attattgcaa aagctcctgc aatataagct
     3481 cctgtttgtc ccgcaaaata tctgccaaac aagacagcaa cgacaccctt taatacgtct
     3541 cctgaaaaaa ccatcgcgcc tacttttttg ccgaatgccc ttattgcatt cgttgttccg
     3601 gcattgccgc ttccaaattc tctgacgtct ttattggtta atatttttcc taatatgtag
     3661 gcaaatgata tattgcctat aaaataagat attattgcga taaaaatata tttcataata
     3721 cctccagcat aaccttatta tctattacac agtttggatc ttatttctcg cttctgtttc
     3781 taaattcaat aataattggt gttcccttgt attcaaaagc ttccctgatt ttattttcaa
     3841 tatatcttcc gtatgaaaaa tgaacaattt ccttatcatt tacaaatatt aaaaacttag
     3901 gaggatttac tgcaacctgc gttccgtagt atattttaag tctcttccct tttactgttg
     3961 ccggttgatt cataagaact gcttctgtaa gaatatcgtt taatactccc gtagaaattc
     4021 ttaaatttct gaagctgtat atttcatcaa ctgcttcaag tattttgttg accctctgac
     4081 ctgtaagagc tgagactgtt aatatggggg catatgtggc atatgctaat ttttgtctta
     4141 tctcttcagt aaattcctta aaagtgctat tatccttttc aattaaatcc cacttgttta
     4201 tgacgataac cattcctttg ttgctgtcat gagaatatcc ggctattctt atatcctgct
     4261 ctgttactcc tttatcggca tcaattacca atacacatat atctgctctt tccactgcag
     4321 taaaagctct taatacgctg tacttttcga ctgattccaa aaccttgctc tttttcctta
     4381 tgcctgcagt atcaataagc gtatacttct tggagtttct ctcgaacttt gtatcgatgg
     4441 catcccttgt cgtacccgga atatcactta cgattactcg ctcttcgcct attattctgt
     4501 tgattattga agatttgcct gcattaggct ttccgataac agcaattttt atgctgtctt
     4561 cctcttcttt tgtatccatt tttccattga attttgaaat aatttcatcc agcaaatcac
     4621 ctatgcccaa ggcttcggca gatgatatcg gaatagggtc tccaagtcct aaattgtaaa
     4681 attcatatac gctgtcttca tttacgaatc tgtccatttt attgactacg agaattattt
     4741 ctttttttgc ttttctaagc atttgtgcca cctcagtgtc agcagcggta acaccttctt
     4801 tgccgtctac cataaacaaa acaacatccg ccatttctat ggctacttcc gcttggatgc
     4861 gcatctgtgc aaaaatcgta tccgttgtgt ttggctctat tcctcctgtg tctattaaag
     4921 taaagtaatt atcaagccat tcgcattcag cataaattct gtctcttgta acaccgggag
     4981 tatcttccgt tatggatatt ctctttccgg caatcctatt aaaaagtgtt gactttccta
     5041 cattcggcct gccaacaatt gttactatag gtcttcccat ttatttcctc cgtttattta
     5101 acgattaagc tgagcaaagt atcatcttct gcatcgtata tttctacctt agtgtttaat
     5161 tctctttcca aatcttcaat taccatatca tccagcatca catgattgtc attaatcata
     5221 tttctaggca atataaggtt tttataattt ttacccttga gttggtctat gatgtctttt
     5281 cccgtaataa gacctgatac ggttattttg tctccaaaat aattatttat aattctaaga
     5341 acattaattt tcacatttat tctgctcata attttttcgg ctaattctac cattaattca
     5401 taagcagcaa ctcctgttac aaaagtaact tcatctttta atactttatt atatcttata
     5461 ttttttatct cttcttctac acggttttta aataacctgc acatacctat cccgttttct
     5521 aattgatcaa aatcctcata atcttcgtag ggaggaaaat catatttact tagaatataa
     5581 aattcatccg caatgaaaat aaatctggta ccgaactttc taagcagtgt ttcctggagt
     5641 tttgtaacct gctcaacaac atcttttgca ctttctttgt cgaaaggctt aagctccgta
     5701 agcttttccc tgtacttggt gagccccacg ggaacaactg caacactcct aagtccgggg
     5761 tgaagagaag acaaatcatt aatggtcttg gtaagctgct ctttatcatt aacgtctctt
     5821 actaatacta tctggcaatc aaccgttata ttggcatcaa tcagcctttt taagtatttt
     5881 aaaatatcca tgtccttatc attgcccatc atatactttc ttagttgagg gtcggttgta
     5941 tgaacagata cttttatggg acttatttta tactgaataa ttctatcaaa ttcatcatcc
     6001 gataaattgg tcagggttat gaaattaccg tacagaaaag aaagcctggt atcatcgtct
     6061 ttcacataaa gtgtttttct catgcctttc ggcatttggt cgataaaaca aaaaatacac
     6121 ttattagagc ataccttaat attatccatc aggggatttt caaatactat tcctatatct
     6181 tcatcgtaat ccttttcaat ttcgagttcc caaatttctc cgtctttttt tttgatttca
     6241 aaataaacca aattatcaga aatttgatat ttataatcta tgtaatcctt tattatggaa
     6301 ttattaacgc ttaaaagctc atctcccggc tctatattta attcttccgc tatgctgttt
     6361 tttaatactt taacaataat gtttttcacg aatttccctt tcaattgata acataagttc
     6421 ttcagtctgt gccaaaagga tttggtttta tcattatacc atatcttaac aaacaactgc
     6481 aatcttattt ttaaacacta atgtcttgca tatttaacaa tttttgttat aatattatta
     6541 tcttttctgc catatgaaat atgtaaagtt aagagctaac taaatataaa gcgaggcgca
     6601 ttatgaaatt caatgagtat gaatatataa gacctgatta taatatcata aaagaaaagt
     6661 atttaaatct gattaatgat tttaaaaact cagcaactgc cgatgaacaa tataccatcc
     6721 taaaaaaaat caatgcttta gcaaataatg ttgacacaat gagtcaactg gtgtatatac
     6781 gaaattccat caattcagct gatgaatttt atgatactga aaaggactat atggatatga
     6841 catcaccgaa attttacgga ttgaggattg aattttacaa gcaactgtta aattcaagat
     6901 ttaaggaaga attaacagat aaaatacatc ctcagcttat taccttagcg gaaatggaga
     6961 ttaaaacatt ttctgatgaa gcgattcctc taatgcagga agaaaataag ctaataagcg
     7021 aatatgataa actgatatca tccgcaaaga tagagtatga cggaaagtta ttaaacttgt
     7081 ctcaaatgca gccctatatg gaatcaaagg accgccgtat cagaaaagaa gctcatgaaa
     7141 aaagcagcaa tttcttcaaa gaaaatgaag ctaaatttga cttgatatac gataaattgg
     7201 taaagattag acaccaaata gcattaaaat taggatttaa taattttata gagcttgctt
     7261 atttaagaaa cggaaggtct gattacaatt caacagatgt tcgaaatttc agagaacatg
     7321 tattgaaata catagtaccg attacgaatg aattaagaga aagacaggca aagcgcatag
     7381 gagtcgatga atttaaatac tatgacattc ccctgaattt catatcagga aatccgactc
     7441 caaaaggaaa taaagactgg ataatggagc gcaccaagaa aatgtatgag gaattgtctc
     7501 ccgaaactca tgaatttata aatataatga ttgacagaga attatttgac ttggaaacaa
     7561 agccaaataa gcaggcagga ggatattgca cattccttca cgattacaaa agtcctttca
     7621 tatttgccaa ttttaacggc actcaagacg atgtaaccgt tgttactcat gaagcgggcc
     7681 atgcttttca aacataccaa agcagacatt ttgatgcgcc cgaatacagt ttccctacgt
     7741 ctgaagctgc cgaaatacat tcaatgagca tggaatttat tacatggcct tggatggaat
     7801 tattttttga aaaagacata gaaaaattta aatttttcca cttggcggat tctttgtgct
     7861 tcatacctta tggagtgacc gttgacgaat ttcagcatat tgtttatgag tatcctgata
     7921 tgactcctga tgaaagaaaa aaagcatgga gagatatcga aaaaaaatat accccttata
     7981 aaaattatga tgataatgaa tttttaaata aaggtacttt ttggtttaaa caaggtcata
     8041 ttttctcatc tcccttttat tacatcgatt atactctggc acaaatttgc tcctatcaat
     8101 tttggttaag gtttaatgag aacagaaagg aagcatggga agattactta aaaatctgta
     8161 aaatcggcgg gagtcaatcc ttccttgaga ttttaaaagc cggtaatcta aaaagcccct
     8221 ttgaagaatc aaccatcaat tcagttgcat caaaaattaa agaataccta aatacgatta
     8281 atgacatgaa actgtaagga ttctggaaat ataaaaggga ctgattaaag cgtatacata
     8341 cacactttaa tcagtccctt ttattaattt attagtcctg ttgaggagcc tccactgggc
     8401 atacgctggc gcatgctccg cattcaatac aagttgcagc gtctatcaca tatttgtcat
     8461 caccggctga aattgcacct acagggcact caggttcaca tgctccacag cttatacatg
     8521 catctgttat tatgtaagcc atttaaattc acctccccca atatggtcat caccacttat
     8581 tgtaacacta tttaaaataa ttttaaagtg ttttttcata ataatttcac accttatata
     8641 aggtgtgaaa ttattaatct tccaattcct ttaattcttc gatatctatg tccgtatctt
     8701 ccgcgtcgta atgtttcagg acaccttctt ccacaacctt cagtcgtgaa ccttgaattt
     8761 cttctacttc aatagttccg tcactttttt ctacattaac ttttgcagtt acaaataaag
     8821 gattaatgtc tattactata cctgttttat ttgttcccaa cacctgtact ttatctccta
     8881 tatccggaag ctttttaata ttttcttcgt atacctgatg ctcgtaattt aaacagcaca
     8941 tgagcctgga gcatattcct gatattttgg caggatttaa agataatttt tgctcttttg
     9001 ccattttaat ggataccgga taaaattcac ccaaaaacga tgagcagcac atatgtctgc
     9061 cgcaaggtcc aagacctcct atcattttag cttcatccct gacaccgatt tgccttaatt
     9121 ctattcttgt tctgaatatt gccgctaaat cttttactaa ttccctgaaa tctattctgc
     9181 cgtcagccgt gaaataaaaa attactttgt tattatcgaa agtatattct atatcaatga
     9241 gcttcatttc caaaccatgt tcttctatct ttttgaagca aatatcataa gcttcttttt
     9301 ctttttcttt attttctcgg tttcttttaa tatcatcctc tgttgcaagc cttagtactt
     9361 tttttaatgg ctgtacaata tcttcttcct taaccatttt tggacctaca acaacctgtc
     9421 cgaattcaat acctcttatt gtttctacta taacaaaatc tccattgtta atccactttt
     9481 tttcaggatc aaaatagtat atcttgccgg cttttttaaa tcttacgcca actaccttta
     9541 tcatttaaag acctcctgaa tctttaaaag catattatct acagtcaatt tatagttaac
     9601 attgctcttt atattatcgc ttgtattttg catatattca attatatctg tatctttctt
     9661 catatttttc ccgtgagttt ttaaaagatt tatataatcc ttgtttatta caagtctatc
     9721 catgttgtta ttcacaaata ttatatctct tatccacatt atcattattt ctattattga
     9781 atttatatca tcttttttat cgtcaaagta actttccaat tcaaaaatta tttcagaatc
     9841 cgaatttaca attttatcaa aaattttaat tatttcttct cgtttcttaa gagtgtcatc
     9901 actgccctta attatattta ttgccttact gagtattcct tttgaataat ttgccgctaa
     9961 ttcaacccta tctgaatcat aatcttttaa atatgacttt attgaatctt tgcttatatt
    10021 tttaaatttt atttcctggc agcgggaaac aattgtaggg agaagcaaat ttgaattaat
    10081 tgtcaaaagt attataactg tatcacccgg aggctcctct aaggttttta agaacccatt
    10141 agctgcctgc ggtgtcatta aatgagcatc ctttataata aaaatctttc ttttagcctc
    10201 ataaggctta ttgtatactg tattaatcag caaatcaata tcttctcttt taatactatt
    10261 cccgtcttta tttataatgt gcaaatcagg gtggttaaga gaatttactt ttatgcaatt
    10321 cttgcattta ttgcatggct ccttatgaaa atttaagcat agaagcgact gggcaaatac
    10381 taatgccaat tcaaatttac ccatgccttc aggtccttca aacagataac aatgagatac
    10441 ttgattgtta tctattgact tgattaaaga tgtttttatt tcttcttgtc ctgctacttg
    10501 gctgtaaagc atacaagccc ccttaaatct tttcaaatct atcaacggga gttacaaaca
    10561 caacagctcc ccctacagtt acttcaatag tttgcggtaa atatcctcct accgtactga
    10621 atgaacttgg aggtatggca gcagtaactt tctttttagt cttgcatatc tttcttatta
    10681 tttcaattgc ttcatcaact ctatcatctt caatccctga aatcacagtt gtatttcctg
    10741 agctcaaaaa gccgcctgtt gatgacattt tagtggatga aatgccattt tctctcaatg
    10801 ccgatatcaa cttatttgca tcatcatttt gtacaactgc agttatcagt ttcataagaa
    10861 cctccttata ttgttaataa gccctttatt tcttcaaata tttcttcaat agacctttca
    10921 gcatcaatgg ttattatttc aggaaaatgt gatgaaagct ttttatatcc ttcatatacg
    10981 cttcggtgaa aagaaatatc ctcgttttcc agcctgtctc ccccgtcaat gcaaagtttt
    11041 cttttcaatg ctttttcagg ctcaacatcc aatagcataa ccaaatcagg cttaattccg
    11101 gctgttgcaa aatcatttat tttttttatt tcatcaatac caaggcctct tccaaggcct
    11161 tggtatacta aacttgaata aacatatctt tcgcttatta ccactttccc tgctttaagc
    11221 cagggtatta tcttttcaga cactaattgt gctcttgatg cggcataaag caaagcttcg
    11281 gttctgtaat ccatttcagt gttatctctg tctaaaatca gttccctgat tttttcactt
    11341 attgaagtac ctcccggctc cctggtaaat tcaatatcac ggcctttttc gctgtaatat
    11401 cttccaattc tttctgctat ggtacttttg cctgaaccgt cggagccttc caatactata
    11461 aatcttcctt ccatttaaca actctcactt gattattttt gttaacacca tttacgttaa
    11521 tgccattttc caacataatt tttacataat ctattgtatc ttgaacgatt atttctccgg
    11581 gacaaacaag aggtattccc ggcggatatg gtattatgaa ttctgccgca gtctttccta
    11641 tagcatcgtc catatgaaga agcatgtttt cggcatagaa tgccttcctc atttttcttt
    11701 catgctccag cttaggaaat ggctcgattt gaacaatttc tcttttttcc ttgcttttgg
    11761 attcctctat atatatttct tttacagcgt caaaaagtct gtcgatatct tcaggctcat
    11821 ctgtagcagt aacataaccg acagtatact gcaagtcagc aaattccatc tgtattttat
    11881 atttatacct taatatatcc tgaagctccg tacctgttat cccaagctcg ctcatgctga
    11941 taaccgcacg agttgcatcg aaatcataca aaccttcgct gattaaaaag tcatcttcca
    12001 atacattgat tcccatattt ctaagctcta atgttcttct tttgaaaact tcaatatttc
    12061 tttttaatct ttcttctccg ttctcagcca tccaatgaat tgcattttca attgatgcca
    12121 tcaaaatata agacggagat gtggtttgca gcatccttag cattgtttca atattgttga
    12181 catttactct gtctgagcaa atatgcaaaa ttgagccttg agtaaggcat ggcaatgtct
    12241 tatgtgtact ttgagctact atgtctgccc cggagttttc cgcaggcggc ggtaaaagct
    12301 ccttgcaaaa ttgaagatgc gagccatggg cttcatctat catcaaaatc ttgccgtgct
    12361 tatgaactat ttctgctgct ttttttacat caaaacaaat accgtaaaat gtaggataag
    12421 ttagcaccac catttttatg tccggattat ctgtaagcat ctggtcaagc ttatctaagc
    12481 taatactaaa gtttaatccg tactttttat taaaatccgg ataaagataa aacagtttta
    12541 aatctcctaa tatacaagca ttgtaaaccg acttgtgaca atctctttgc acaagtattt
    12601 tatctccggg gtttgtgcat gccataattg ccgagataat accacatgat gtaccgttaa
    12661 caaccatgta cgacttttta gtaccgtata ctttggaaat ataattcatt gcatccttta
    12721 aaaagcctgt aggataatgt aaattatcag ttccaagtgt ttccgtaatg tcaaaaaaat
    12781 taagattttt ttcgagttca cctaaaattt ccggttttcc tttgtgtccc ggcatgtgga
    12841 aatttgtatc tacttcactt cgatactgtt taatgcctga aataatatta agttttttca
    12901 tctttttttc ccgttaataa aatatatcct tctatttatt gggattagta ctcagctcct
    12961 ttaagattat agttttttat ttctttttct agaaatatta tttgtggtat caagagtttc
    13021 aactgtaagt accactgtta ttgagaaaaa cgtcatagca attgaaaata gtacaatata
    13081 ttcaagccac atacaatcaa tcaccttcct gtacatatta tataacagca cattaccatt
    13141 gtccaataaa acttgaatat tctatataag cagttttacc aacaatatag caaaaaatcc
    13201 aggtacacct aatattccta taataagtgc ggtgaattca tttactccta tatgaacacc
    13261 ggtatactgt ccgaaataat tgaatattat taagactata gcgcctatca ctgaatttaa
    13321 aattattttt cctataaatt taagcggttt aaaaaatatc caggatgtca tgaatacaag
    13381 cattatccct gccgtataag ctaatattat acctaaatct gctccagtca caaaacaacc
    13441 tccattacta aataatccgt taacccggtt tattaatttt atgaagttgt ccttatattt
    13501 atgtcatcta tatacattgc agacatcttt actaaggctc agaattttcg tgtgtttcta
    13561 tcagctcttc cttatcccca atttttgact ttagcttttc aggaattttt acatatttat
    13621 taacaaaaag agcaattatt ataccgattg aggtatccaa aactctgtta aaggtatata
    13681 ggatataacc ttcatcatga ttcaaggtta taactaaaaa tacaacgcag caaataacta
    13741 ctgctttctt catgttaatt gttacgcaga tttgaattaa caacataatt cctaatggta
    13801 taataaaaat caaaatttta tgattaaaaa aagtatttac gataaacaat gtaaaggctc
    13861 ccgccaggcc tcctattatt gttccgataa tccttgaaaa tcctatttcc tttgagtcaa
    13921 caatcgtatt ctgtacacag attactgcag ctatacatgc aaatatggga ttatttctgt
    13981 taataagttc aaaaaccaat aagcatataa agactgaaat tgttgttttt atattgcgca
    14041 tacctatttt tatcacgacg tttcctccgt ggaatcataa taacattttg atttttaaac
    14101 atattgtaat taataattat aaatttgtcc agtaatatat aataatattt tacacatgtc
    14161 ttaaaatgta ttataatgaa tgcaaacaat gaagcaaaag ctctggcttt tgcactgatg
    14221 cattcattgt atcggtgtga agcaagccga ttttgcggct tgcacttatg ataaatagat
    14281 ttgaattcaa aattaaggag acaaaaatgg aaagaatagt gggaactgtt gtcagaggat
    14341 taagagctcc aattattaca aaaggtgatg atatagtaaa aataactgtt gaaactgtgc
    14401 tgaaggcttc caagactgaa ggcttttcat taagagataa ggatatagta ggtattacgg
    14461 aagcaattgt agcaagagct caaggtaatt ttgcaaatat tgaacaaata gcgctggatg
    14521 taaaacaaaa atttgatagt gatactgtag gtctaatatt tccgatttta agcagaaaca
    14581 gatttgctgt ttgtcttaaa ggtattgcta aatcattcaa aaaaatatat ctgatgttca
    14641 gttacccttc agatgaagtg ggtaatccct tagttgatta tgacctcctt gataaggaag
    14701 gagttaatcc ttggacagat atacttaaag agaatgaatt caggagccac tttaagaata
    14761 caaaacatat cttcacggga gtggattata ttaaatacta ttcagaaatt ataagagaaa
    14821 gcggatgtga agttgaagtc atatttgcaa ataatcccat aagtattttg aattatacaa
    14881 agaatgttct tacctgtgac atccatacaa gaaacagaag caaaagattg ttgaaatcaa
    14941 atggcgctga aaccgtatac ggcttggatg atattttgaa tcaatctgta gaaggaagcg
    15001 gtttcaatga aaagtacgga ctgttaggaa caaataaggc tactgaagat accgtaaagc
    15061 tttttccgag agattgccag tccttagttg aaaaaattca agaaaaactt tttgagctta
    15121 caggcaagaa aattgaagta atgatatacg gggatggtgc ttttaaagat cctgtcggga
    15181 aaatttggga gttggcagac ccggttgtat ctccgggata tacagaagga ttaatcggta
    15241 ctcccaatga atttaaactt aaatatttgg cagacaacca atttccgcac cttaaaggcg
    15301 atgaacttaa aaaagctatt tgtgattata ttatgaataa ggactgtgat ttaaaagaca
    15361 ggatggaatc tcaaggaact actccgagaa gacttaccga ccttataggc tcactatgcg
    15421 atttaacatc gggaagcgga gataaaggta cacctatagt atatatccaa ggctatttcg
    15481 ataattattc ggtataaaaa aaagggcaac gccctttttt tatttgggtc atctgaagat
    15541 ccttcgcttt cgctcaggat aagcgactga caccaaatca tagatttggg tcatctgctt
    15601 acttgaagct gtactgtgat attaagtgct gtttgcagtc ccatagttct ttagagaggt
    15661 caacatagta atggtgagga tttgaaaatc tttttatttc tccccataag ttgcctacct
    15721 tctttttgca atattccctt atttcattta agcttggact ttcataaacg cattcgccgt
    15781 ttataaaaac agggacctga agattttttg cataaaaact tgttactttc ttcttcttcc
    15841 aagtatgtat ttcatcaaat attgtataag gctttgaatt atctattatt tcatcagcca
    15901 gtgttattac atctgcaata ggactgtttg tttttttgtc atacaatctc catacctttt
    15961 tgtagccggg attagttatt ttttcttcgt tttccgatat tttaatcttt ggaataatct
    16021 ttccgtccct ttcaaccgcc actaacttat aaacgcatcc gaacacaggc tctgacttag
    16081 ctgtaattaa tctttcccca acgccaaagt aatctatttt agctccctgg tccagtaaat
    16141 cagaaataat aaattcatca agactgtttg atgcaactat tgcacagtca tgcaaccctg
    16201 cttcattaag cattttcctg catttcttgc ttaagtatgc aatatcgccg ctgtcaatcc
    16261 taactccctt aagtcttttt cccataggct ccaatacttc tttggatatc ctgattgcat
    16321 ttggtattcc gctttttact acattgtaag tatcaaccag taaattgcaa gcatcaggat
    16381 aattttgtgc ataagattta aatgcttcat attcgctttc aaacatttgc acccatgaat
    16441 gagccatggt tcccagtgcc ggaactccaa aggcttcatc tgccaatgtt gtggctgttc
    16501 ctattactcc gccgatataa gcagctcttg ctccataaat tgctccatca tagccttgac
    16561 ttcttcttgc gccaaattcc attattggct taccttttgc tgctctaacc actcgatttg
    16621 ctttagtggc aattaagctt tgatgattta ttgttaaaag caccatggtt tcaattattt
    16681 gagcatcaat tgtcttggct cttaccgtaa ttaaaggctc attgggaaat acaggagtcc
    16741 cttcaggtat tgcatatata tcacctgaaa atttaaaatc tttcaaatac tgcaaaaatt
    16801 cttccgtaaa taattttttg cttcttagaa aattaatatc ctcttcataa aattttaatt
    16861 cttttatata ttgaattatt tgctctaatc ctgcaactat tgaaaaccct gcaccatcag
    16921 gattttttct gaaaaataaa tcaaaatata ctatttcatc cttcattccg ttagaaaagt
    16981 atccgtttgc cattgtaagt tcataaaaat ccatcagcat agataatctt ctttcgccaa
    17041 aattattata atacataata tactcctgtt tttatcttcc tatggtttat tttatcacag
    17101 attatacatt ttgtatatta tagtgccaaa atcatttttt gtcaaaaaat agatatctac
    17161 ccattgattc tcaatcactg agtagatatg atgcttgtta tactgcttta tcttttattg
    17221 ataaatctcc ttctattacc ttagtgggac atttttctac gcaaatcatg caatgcgtac
    17281 atttgtcata atctaccttg gctaaattgt ttgtaacgtg tattgcatcg taaggacagt
    17341 tcttttcaca aattttgcat gctatacaac cgattttgca aacatctttg actgctttac
    17401 cgaagtctct gttgttgcat cctacaataa cattattctt atacggaaca aattctatta
    17461 cggcttttgg acattcttca atacattttc cgcaagcagt acatttttct ttgtctatta
    17521 ctgctattcc gtcaattata tcaatagcat caaaatcgca tactctgaca caattcccgt
    17581 aaccgaggca tcccttgtca caggacttat ctccgccttg aacagccgct gctgccttac
    17641 aatcccttac tccatgatat tcatgttttt ttgaagccag gcagtcattt ccacgacata
    17701 aaacatgagc aacctttttt tctccctctt cagctgaagt acccataata tcggctattt
    17761 ttgcatgagc ctgataattt gccaccggac atccgtcaac aggcgctctt ccagctgcta
    17821 ttgcttcagc catagcatca cagcctgcat atccacaggc accgcaattt gcacccggca
    17881 aagcatttct tacatcggta attctttgat ctacatctat atgaaacagt ttagctgcaa
    17941 gagccaataa taatccgcat atggcaccta tgccgccagt gactgctact gctttaacta
    18001 taaccatctt tttacctcct ataactgcaa tcctgaaaat ccaaggaaag caactgacat
    18061 aagccctgca gtaatcaatg aaatagcaga gccttttaaa gattcaggca aatcagcata
    18121 atcgacctta tctctaactc ctgcaaacaa tactattgcc agcaagaaac ctgcggatat
    18181 acctacagaa taaactaatg attcaacaaa attgtactca taaataatat tatttatggc
    18241 aacacctaag actgcacagt ttgtagtaat aaggggcagg aaaattccca acgcctgata
    18301 caaaccggga gctgatttct taagaaacat ttcaacaaat tgtaccaatg cagcaattat
    18361 caatatgaat actattgtct gcaaatattc caattccaaa ttaaccaaaa tagcataatg
    18421 aataatgtat gttattacgg atgccaaggt tataacgaat ataaccgcaa tacccatacc
    18481 ggctgcagta tcaattttct ttgatactcc gaggaaagga catgttccca aaaactgcat
    18541 gaatataata ttattaacta taaatgatgt tataaataaa ctaactaatt ccattcccta
    18601 cctcctaagc tttctttctt cttgaaagat agtttactat acctatcaac aacccaagga
    18661 gaataaatgc tcccggcggt tgaattgcca tcatcatagg ctcataagct tccggaagaa
    18721 ttctaaatcc caaaattgta ccgttgccta atatttctct tacagagctg agtaaaatca
    18781 tagcaactaa gtatcctaat cccattccca aaccgtctat tatagagtca gacaagctgt
    18841 tttttgaggc aaatgcttca gctcgggcaa ggataataca gttaacaact attaacggta
    18901 taaatacgcc taatgcttta tataattcaa atgtataagc atgcatcaat aaatctatta
    18961 tggttgtaaa tgctgcaatt actataataa atgacggtat tctaacctca tcgggtataa
    19021 atttacgcaa taaagatatt actatatttg aaccggtcaa aactgctatt acagctacac
    19081 ccattcctat tccgttaata agcgatgttg taattgccaa ggtagcacac ataccaacct
    19141 gttgaactaa aataggattt tctgttgtta ctccattttt aaatatttct aatttattca
    19201 tgtcatcctc ctatttcaca tattccgcat aaacatttat agcattgttt acggcactta
    19261 aaaatgattt agaagaatac gtagcgcctg aaacagcatc atagtcacct tcagaaattt
    19321 cgccgcttgc atccttgcca ataaactgtt ctgtaaatgc aagtagagta actttagagc
    19381 ctactccttc agtttcagca tgtgatatta cacttgttcc ggatatttgt ccatcagggg
    19441 tgaaaccaac atataattcc aagtctccgt tgaagccttt aacagtactc catgttctta
    19501 taactttccc aagttcgtta cctgattcat caacagctgt aagaagatta ataaatttgt
    19561 tattatcagc tttaattgta tctaccaatg cagtatcttc ataatcatta aacattacag
    19621 atccgggcat aactgcttcc aatatagccg ggtccatact tgccattgct tcattttgtg
    19681 caattctatc acttgttacg tcattcagcc atgctaatac tcctgctgat accgcaccta
    19741 taataagcaa tactcctgca agttttacaa aattattttt catttttttt acctcctgtt
    19801 gcaactccga atacgcgagg cttaacatat ttatctatca ttggagtcaa aatattcatc
    19861 agaagtattg aataagaaac tccttcggga tatcctccca tttttcttat aaacattgta
    19921 agcattccgg ctccaactgc atatataact tttcccttat cggtaatggg gcttgttgca
    19981 taatcggtag ccatgaaaaa tgctcccaac atcaaacctc cacctaagat gtggtatgat
    20041 aaattgccag catcaaaaag taatgctatt gctgcaactg tacctatata aaacgccgga
    20101 attatccatt tgattgttcc ggtgtaaatt aaatatattc caccggctaa taatagtatt
    20161 gccgaagttt ctccgataac tcccggtata tttcctatta acatatccca caatgacggc
    20221 agttgtccgg atgctgcacc cttgataata gaaagaggtg tcgcagaagt aactgcatca
    20281 attgccgggt caataaatcc tcctgacaga tgatgaggcc atgatgccat taagaatgct
    20341 cttgctccca atgccggatt taaaaagtta aaccctattc cgccaaatat ctgttttact
    20401 attcctattg caaatacaga gccgataact gctatccacc atggtgcttc tgcgggtaaa
    20461 ttaaatgcta agagtattcc tgttacaacc gcacttaaat cggtaactgt tattggtctg
    20521 ttcataaatt tttgacaaaa atattcggta gcaactgcag aaattacaga tgctaatacc
    20581 aatatcattg ctttatatcc aaaataatat atgcttgctg ccaatgcggg aattaatgca
    20641 attatcacat caagcattaa tttttgtgtt gtttcatcgg atctaatatg aggtgacgat
    20701 gaaccaatca atttaatttc catcaatatt tcctcctatt tagccttttt gcgtctggtt
    20761 aaaatttctt tttttgcaac acttatcgat tctttaagcg gtctctttgc aggacataca
    20821 tacgaacaag caccgcattc tatacaatca gttgcatgat attcatcagc accgtcgaaa
    20881 tttctcaata atgaaaatct tgctattttg taaggcatta ggtatacagg acaagcctca
    20941 acacatttgc cgcataaaat acaatttgaa tgctccggta catttgcttc ttttttattt
    21001 aaaaccaata ttcccgatgt tccttttata acagggactt cgtcagtata ttgggcaatt
    21061 cccatcattg gtccgccgct gataattttt cccggctctt ctttgtaacc gccgcacatt
    21121 tcaatacaat ccttaaacag agttcctatt cttacgtata ggtttttagg gttttcaaca
    21181 gcacttcctg ttacggttac aactctttga atgcagggca tacctgtttt tattacattg
    21241 cctattgtag caactgtacc gacattttct acaacacatc caacttccat tggaagtcct
    21301 cctgaaggta caactcttcc ggttaccgca ttaattaatc ttttttcatc gccttgaggg
    21361 tatttagcta tcatcgatac aacttcaata ccttcctcat tgccaacagc ttgctgcaat
    21421 gtttcaattg catcaggctt gttatcttct atacaaatat atgctttatt tactcctaat
    21481 gccttcataa cagcctttgt gccaagcaat acaagatcag gtttttccaa cataattcta
    21541 tggtcggcag tcaaataagg ctcacattcc gaaccgttta ccagcaaggt atcaatggtt
    21601 ttgtcttttg gaggacttag cttaacatga gtggggaaag ttgctcctcc cattcctgcc
    21661 atacctgctt ctttgattat agaaagtatg tcttctttag aaagatcttc taatttgcct
    21721 tttggctgaa ctgattcatg cacttcatat ttcccgtcgg attctataat aacgcaattt
    21781 accttcgctc cgcccggcac ctgtcgattt tcaatctttt ttactgttcc ggacacgctc
    21841 gaatgaacgg gtgctgaaac aaaggctttt gattcgccaa ttttttgtcc aacttttact
    21901 ttatctccta cagccaccaa aggatcacat ggggctccaa tatgttgcga caatggaatt
    21961 acaacaatat caggagccgg tgcgtcttca attggcacat tctgcgtaaa agacttactt
    22021 tgagggggat gaattccacc cttaaacgta aacaattttg tgctcattat accacctcgt
    22081 gaaattaata attatgaatt ataaaaaagt tataatctca aacataaaga ttataacaga
    22141 ttgttttcga cttttaaata ccttttttta tttattttta taattttaac attatttatc
    22201 aaattatgca agaccataaa ttatcaaatt ttacctgccg atacaaggtt tacattttca
    22261 ttgatatatt ccttaatcca cattaaaaca ccgatgtcgt taatattaat accttcaagc
    22321 atttctttta cttctctcgt atcaaaaata aattctcccc tgtcagtctt atgagaataa
    22381 gttagttcaa aattatcacc ggagttaata attgtcataa ttgtttcccc catgttaccg
    22441 aggggtgctc tgtctatatt atccctttca aaataacagc aaacctttgt tccgacgcct
    22501 atctttgatt caatttttaa atagccgtta catctttcgc atgcttcttt cagcattgga
    22561 attcctaatc ctacttttct ggtagtgcga gttgtataga agggattgga tactctgttt
    22621 ataacatctt cgctcattcc tctgccgtta tccgcaatag ttatacttaa ccggttattt
    22681 tttatactat cttctatatt tatttcaatt aatgaagcat cagccttaat gctgttcatt
    22741 gcaatatcca atatgtgcat tgaaatttct ttcataattc ctccaaccaa gatattacgc
    22801 agtggaatgc aattggggac attcctctga ccgacatagg ttatcccaat agaaagaggt
    22861 gtgtccccat acctctatag gtgtgtcccc cctcctttta gtaaatacgt ggacattcat
    22921 tcaaataaat actcataaat ctcttcgata tcatccagat caaaataatt aaccgcttca
    22981 gctatatcaa caagctgatg ggcgtccgaa ttataaatca ccttatattt ttctgctatt
    23041 ttactgttaa taggcatttt tgaatatact tccacagtct ctattttcag attgataggc
    23101 ataaaaccta ataccccgag aataccgttt gtcattttgt taatatgggc aggaatcatc
    23161 actccatcat atttcttgac aatggaaaaa atatcttcga tggtatattg cgaagcatta
    23221 atcaataatt tttccaaggt gcctgttatt tcatccttag aattatatat attttgctca
    23281 ccgaatattt caggattatt ttttataaaa ggtaatgaat tataaataat attgccaaat
    23341 tctattgcat tatccaattc tttaaagtag caaagcacgt gtacttcttc ctttgttgtt
    23401 acttctaatc cgggtactac ctttatgcct gttttattgc ctaattccat tactgcacgc
    23461 aaatttcccg ctgaattatg gtctgttaca gatattatat ccagcccttt tattaaggac
    23521 atatttataa tattatttgg agtcatatcg catgcgctgc acggagacaa atccgaatga
    23581 atatgcaaat cataataaaa tttcatttta ttcccatatc tgacaatctg catgctactt
    23641 ggtatgcact ttctgatgtt ttaaatatag gtatgtttaa ttcctttgct ttatccaaag
    23701 tatcattgtc gactgccata ttttcaacaa ctataataca agaaagctcc aaaagctcgg
    23761 caatagctac aatattaata tgggtttgaa tagttatcca tacatagttc ttttttgctt
    23821 ttgccattac aatacttagt aaatcaccta tatacacacc ttcaatttca atatctcctt
    23881 ctttttcaca cagtggtgta agctgcagtt tttctgcaaa ttctaagcag ttcattttcc
    23941 cctcctctta tttttttgct ctcagtacaa cacagtcata aaattcagac tcgttattat
    24001 aaacgtcttc agcgtgagcc aaacatgtgg gtgctccgca ggagccgcaa tccaatcccg
    24061 gaagtttatc aaggatttcc cttatttttt cattcctttc aacagcttcc tttaagttaa
    24121 gtttgtcaaa tttttcttct tccttcagct tataattaaa gaagccggtt ttatacattt
    24181 cgtcaaatct gtcgatatca ggatttaaac attcatcagg cagactgttt gaaatataat
    24241 taatgctgct gcttgcaata aacggattta caacattaaa ggttccgcct acacaaccgt
    24301 taacacaagc tgaagcttcc acaaaatcta tatcgcttaa tttaccgttt tcgatttcct
    24361 ctaagatctc aatgatgttt tccatgccgt gaacggctat ataattattc aattccgcat
    24421 gttccgactg accgcctgcc actgcccatt tcatacctaa tagtgacggg tatgagcatg
    24481 taatattatc ctctcttata acttccctgt acaggtctcc gtatattttg ttaagaggta
    24541 ttacccaatc taccgaaggt ttgtagtctc cgattggatt cgttaccgcc aacaacttgg
    24601 caggacatgg tgatatataa aatactttta tatcttcatc ctttaaaccg aactttttcc
    24661 tgactctttc ccttgcaagt attcctgcaa tttccatagg ctcccttatt aaaataagat
    24721 tatccatcaa tgaagggtac ctgtatttaa taagcctaac cgctgccgga caatatgttg
    24781 atatataagg tctcattgtt ttttctttat ctattttaag ttttatggct tttgcaacca
    24841 aatctgccgc ccagcttgca tcatacacaa aatcaaaacc taatttcaaa attgcatttt
    24901 ggaccttgca tatatctgtt cccctgggga actgcccgta taaagaagtg gtaggaattg
    24961 caattttgaa ttttttgggt gtatcatgca tttgattgta ccactctatg gattcgggag
    25021 aataagcatt ataagggcat atttttatac actcaccgca atgaatgcat tttgccgtat
    25081 ttataaatgc tttcccttct attaatctta tagcctgagt aggacatctt ttcatgcaat
    25141 tggtacatcc tctgcattta tctttgtcaa acataattga aaagttcata ataccctcct
    25201 acaaatagat tgtcgcaatt attgttgtcc cggtactact tgtattgatt tctaattggt
    25261 cagaatattt tttaatattt ggaagtccca tacctgcacc aaatccaagc tctcttacac
    25321 tgtctgtcgc agtggagtac ccctctgtca ttgccatatc taaatccgtt attcccggac
    25381 ctgtatcttc cgaaattatt actattttat tttcatatat atcgcagtga agtttcccgc
    25441 cgtaagaatg tataacaata tttatttcag tttcatatga tattattgat atttttctta
    25501 tagtattcga atcaattccc aatgatttta aattgctttt tatggcactt gcacttttgc
    25561 ctgcagacat aaaatcattt gctgttatat tgtagctgta tctttttaaa aatttatcag
    25621 tcatttattt ctagcccttc cattccgttc ataaccaata atccgcatgt tttaaacata
    25681 gtgtaatcgg ttgcaagaat tgtaataccg ttttcctttg ctaattctat tacttcttcg
    25741 caaggtcttt tcccccttac aaacaatacg gcattaatat ccagcatttc aacagtccta
    25801 ataacctgcg gattgtttaa ccctgttatc agcaacgaat cgctgcgtcc atatgcaagt
    25861 acatcactca ttaaatcact ggcaaaaccg aatttaaatt cggtattacc gtcatgatag
    25921 tttgtaagaa catctgcttt tacaatatca actatttctt ttatttgcat accatccctc
    25981 gctatttaat ataattttca atgtgggctt ttccgcctac gtatacgtcc ataatatcat
    26041 tatgagaatc aattcttaca aagataagac ttttttggtt cattgattca ccttgaatta
    26101 cttccatttg atttgaaaac cctttactga aaagtaagct tcccaatgct ccgttggatg
    26161 tacctgttgc acattcttca tttataccca ctcttggagc gaaatttctc gcataaactc
    26221 tttcattgtc aattgtaaaa acatgatacc caactacatc attttcataa gaaatatcct
    26281 ctatcatctt gaaatcaaca tcaagactat ttagcacctt cctcgagcta accggtacca
    26341 aaatgtcttt taaacctgta gaaacaattg taggagcaat atcataattc ggcagaccta
    26401 ttaatctttc atctatattc agcgaatctg ctatttgttt tttctcttta agctcaccgt
    26461 atatacgtgg ttcgctttgc tgcataagaa catattcaac atcttgtcct ttaaatgtta
    26521 tttcaatttt gagtctgcct gccttcgtat tttgatacac gcattttgtt tcatcgttac
    26581 ctttaatttg tccaatttta cccaagtaat aaaaagctgc aattgtagca tgtccgcaga
    26641 ggtctacttc gcaaacggga gtgaaaaaac tcacatcata atcgttttca tcccgtttat
    26701 aaataaacgc cgtctctgat aatcctactt tatttgcaat ttcttgcttc ttatcatatg
    26761 gtatctgttg attcaaaaca acaacacctg ctccattgcc accgctgttt tcataggtaa
    26821 atgattttaa aattgcaact tccattttcc cctcaccgct ttattatata atcattataa
    26881 ttcaaaagca gtataattac aagcaaaaat acattatcaa tctttaccct tatcattcta
    26941 tgaatgaacc gtaacaaaat tacacagata aaacaaatag tttcggggat tacccgaaac
    27001 tatcttagtg cggttggggg gatttgaacc cccacgaata tacattcgct agcccctcaa
    27061 gctagtgcgt ctgccgttcc gccacaaccg cattacctat aatattataa tagattgatt
    27121 aactcttgta aagtaatttt tcagtcaatt aagccatcca aaaaatacct gaaacagaaa
    27181 aattgatttt taatttat
//
LOCUS       DAOPUE010000016        26155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1857_length_26155_cov_10.705402, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000016 DAOPUE010000000
VERSION     DAOPUE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 26155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26155
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1857_length_26155_cov_10.705402"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1158
                     /locus_tag="PKH42_02810"
     CDS             64..1158
                     /locus_tag="PKH42_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA19390.1"
                     /translation="MKLKFIHTSDLHLGKKFDIKNFSLKERKKRRQELWDSFDEIIRT
                     AKIEEVQYLFIAGDLTDGEYISYKSLNKISSKFSSIPDTNVIITCGQSDPYNVNNMYE
                     YIEWPDNVYIIKNTETVEKLNFPENNLCIYSISWDNHAENKRTQPIYDISVDENKLNI
                     LMLHCDIDSSDRLPVNIDIIKNKFDYCPLGGRHNFRQITDNVVYCGTPEPLSFDEEGE
                     HGIIRGTLEKNNTQYSFLPIAKRKFINRQIELNISYSFNKILDLIKFSGDTFSNIKDY
                     VRIELTGEVNADISMDEIENEAKQFFYYIEFEDNYIYDTENRNYEGNEFNIIESYKLQ
                     FDKNDDNDDKLQQQAFKLGLEVLRKEKVVK"
     gene            1160..3835
                     /locus_tag="PKH42_02815"
     CDS             1160..3835
                     /locus_tag="PKH42_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19391.1"
                     /translation="MYINRLNLRAFGKFINRKIHFSNKFNIIFGENETGKSTIHNFIE
                     LVMYGFDEDTSRYNKYKPWNSPVYKGSIEIADEEKKHIISRDFLLDTIQVFKKTNETK
                     ENEYSVEGIGVPGEHFFNINRVSFNNTVSISQLGNKTEKELADELKNKITNLSNTKDE
                     DISIDRIMQSLYRIKEEAGSENNDKTLLGQYSLRLEELRNAKESSMNSNRQVMFLAME
                     KKKLQSKIHELNMKIEEKQKEISDYELFLEKQKFLRAQPVKKEIDNINDELKLIEDDV
                     TNVSAEDYKEASETESSLASMKNDKQRLIEEKEECISELELIESDLSNYIPEEFDIDK
                     LNIDYDIYKANNEKIDTLKMKMKTGRESINSINIDEINNFINDYREVEELNHKIEINN
                     VLTDSKNYELMKKFGKKQRGISLFTGLLGTAFISAAAVSCYFAYFYNNFNYYYGGIGF
                     LIGILFYAFSWRKRNIAINAKKEIESMECEFADYTLSNNELNKQKDEILKRNNCQDFN
                     SMADKFQEKSKEKNIYEEKIKLLNYDDITFNEISADNKKIEERLNNNLKTLNLSLSED
                     TIIEINEAYKRKDTVKLQTEELNKKIEQINRDITRLDKEINFEQRRLEMILQANGADD
                     VEVFKKAVELNEKYKELLNRREYYEKILENIIGSTDYYELKKRTLNVSDEVKEIDKQD
                     IQLSIFKLNEEKTKLLQNINDIHHEIRDIEDNTRSLAEIEEEISFYEEKTAFFKNKIK
                     VAEIAAEKITKISDSIKGDFMPLLRSSISDNFSYLTGGKYKSVDIDENMNITVMSEEE
                     KDRKIELENLSGGTLDQLYFSLRIALSNILSGNQNIPLILDDSFIQYDSKRLRKSLEM
                     LSRESERRQVILFTCQEREAELSKQMNIKFNYFKL"
     gene            3844..4533
                     /locus_tag="PKH42_02820"
     CDS             3844..4533
                     /locus_tag="PKH42_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA19392.1"
                     /translation="MRIFAIADLHFDFKKEKPMDVFGDNWENHEEKIIENWKNTVDEE
                     DLVLIPGDISWALKLNDALEDLIRIENLPGMKIIGKGNHDYWWSTISQLDRLNLKTIK
                     FLKNNFHKYDDVVICGTRGWDTMEEYCAEVDNEKIFLRELNRFKISLQAAKKVSGKKI
                     IMLHYPPFNNNGLPNEFFSLMKDYNADICVYGHLHGEEGHRNVIEGLIDNIMVHCVSS
                     DYTGFKLKRIL"
     gene            4697..>5877
                     /locus_tag="PKH42_02825"
     CDS             4697..>5877
                     /locus_tag="PKH42_02825"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOA19393.1"
                     /translation="MFKAREYYSDILDEIKKSGLWKEERIITTPQRSIISTTKAGGVL
                     NMCANNYLGLADNEEIIEAAKNAYDKWGYGLSSVRFICGTQEIHKELEKKISEFLKTE
                     DTILYSSCFDANGGLFETITTENDAIISDELNHASIIDGVRLSKAKRYRYNNNDMESL
                     EEQLKQADKDGANIKLIATDGVFSMDGIIANLEGICDLADKYNALVMVDDSHAVGFVG
                     KTGRGTPEYRNVMGRVDIITGTLGKALGGASGGYTSGRKEIIDLLRQRSRPYLFSNTL
                     APAIVGASLKVLEMLMEDNKYKAKLDENTKYFREGIKKIGLNIIEGEHPIVPIMLGDA
                     VLSQKMAEKLLEKGVYVVGFYFPVVPKGKARIRTQISAAHSKEDLDFALNAFKDVKEE
                     LG"
     assembly_gap    5878..5977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6286..7230
                     /locus_tag="PKH42_02830"
     CDS             6286..7230
                     /locus_tag="PKH42_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOA19394.1"
                     /translation="MSDKGLKEVKKTSIGGQALIEGIMMKGPSKIATAVRKPDGEIVI
                     KESNIKQIFKHKFFKLPLVRGSFVLIDSLVNGVKELMYSAEFYGEDYEEDALDRFLKK
                     VFKDKADTAIIYTSVIIALVFSIAIFILGPTILTNLLKNIIKNSFLLNLVEGIVRVSL
                     FVGYVYLVSKLEDIKRVFMYHGAEHKTIYCYEYGEELTVENVQRYSTLHPRCGTSFLI
                     NVLLISIIVFSFFGWPNPLLRLVIRLAMLPVIAGLSYELNRYVGRSSSDNIFTKIIAY
                     PGFLIQKITTKEPDDSMIEVAIAAMKRVIPQGGEDDRW"
     gene            7224..8075
                     /gene="prmC"
                     /locus_tag="PKH42_02835"
     CDS             7224..8075
                     /gene="prmC"
                     /locus_tag="PKH42_02835"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOA19395.1"
                     /translation="MVTIEKLLKDGIDIIKQREFNSPKLEVELILCYLLQKDRIYLHL
                     NRKEEVSEEIKDKFYKLAEKRNNGYPLQYITNSQEFMGLDFYVQEGVLIPRPDTETLV
                     ETVINIVKERYNKEIKILDLGTGSGAIALSLAYYLRNSFVTAIDISDIAVETTKINIK
                     KHNLNNIKVIKGNIFEDILGEKFDVLVSNPPYIESDEIKNLQTEVSVYEPRLALDGGV
                     DGLDYYKPIVELFKKICEENGVLAVEIGRQQKKSVEEIFLNSKVFKEVKAYRDLAGND
                     RVIIGMT"
     gene            8180..>9229
                     /gene="prfA"
                     /locus_tag="PKH42_02840"
     CDS             8180..>9229
                     /gene="prfA"
                     /locus_tag="PKH42_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOA19396.1"
                     /translation="MLDKLEALKDKYTELSEKLSDPDILSDSQKLQKLMKEQAQIEPI
                     VLKYNEYKDIRNQLEESKEMLKEKLDDDFKEMVKEEIKELSSKVEELENEIKILLLPT
                     DPNDEKDVIVEIRAGVGGDEAGLFAGDLLRMYLRYAERRGWKTEFISVNEQGIGGYKE
                     VIFSIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSSATVAVLPEADDIDIEINPN
                     DVKVDVFRSSGNGGQSVNTTDSAVRLTHIPTGIVISCQDEKSQLKNKDKAFKVLKARL
                     YDKYLQEQTDELAQSRRSQVGSGDRSERIRTYNFPQGRVSDHRIGLTLYKLDAFLDGD
                     ITEMIDALITSDQAEK"
     assembly_gap    9230..9329
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9439..9684
                     /locus_tag="PKH42_02845"
     CDS             9439..9684
                     /locus_tag="PKH42_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOA19397.1"
                     /translation="MRIDKYLKSARIIKRRTIAKEACEQGRVLINGKIAKPGAEVEIG
                     DEIDIIFGTKPMKIKVEKLLEHTVKDEYKQMYSIINV"
     gene            9777..10313
                     /locus_tag="PKH42_02850"
     CDS             9777..10313
                     /locus_tag="PKH42_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOA19398.1"
                     /translation="MELKGSKTEQNLMTAFAGEAQAHVKYTYYQSQAKKDGYVQISKY
                     FEETARNEKEHAKLWFKLLHDGIAHTSVNLQDAADGEHYEWTEMYAEFAKVAREEGFD
                     KIAYLFEGVAAIEKSHEERYLALLNNIETGKVFKREGEVVWQCDNCGHIHVGSQAPKV
                     CPVCDHPQAHFQLYTKPY"
     gene            10455..11381
                     /gene="murB"
                     /locus_tag="PKH42_02855"
     CDS             10455..11381
                     /gene="murB"
                     /locus_tag="PKH42_02855"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOA19399.1"
                     /translation="MEYQLIINSMLDELTQSFRGLVLKDEPLKNHTYFKIGGPASIFA
                     IPEDTEDLKTVLKLIKKYEIDYYIIGNGTNLLVSDDGFKGIIIKIGEKFNKLSREGNK
                     VTAGAGLLLSTLAKYFAKEGLGGFEFAGGIPGCLGGAIPINAGAYGGEMKDVVTKVKC
                     MDMDGRIHEFSNEEMEFEYRRSRLSDSEYIVLEAEISLTPGRVEDIMEKMKELNEKRI
                     SKQPLNLPSAGSTFKRPLNGYASKLIEDAGLKGLKYRGAMVSDKHSGFIVSYDNASCE
                     DVLELMRIVISTVYDKFGIILEPEIKIVGTKL"
     gene            11422..12162
                     /locus_tag="PKH42_02860"
     CDS             11422..12162
                     /locus_tag="PKH42_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOA19400.1"
                     /translation="MKLRADYHIHSTYSKNRHGKSSIEEIAERAYELGLEEIAIADHG
                     PAHYLFGIKSNLISEAKNIVVETRKKYPNLKILYGVEANILDYKGTTDINSEILKHCD
                     IILCGFHLAALFSSPSDFWNFIVKNQLAKNNKKLYHEMVEKNTQAIINAMNKYKINIL
                     THPGDKIPVNIDKIAQTAEKTDTMLEINNSHGHLNKDEIIRASKYNVQFVINSDSHIK
                     DNVGRCDNALRAVEGSGIDLNRIVNLKS"
     gene            12174..13031
                     /gene="rapZ"
                     /locus_tag="PKH42_02865"
     CDS             12174..13031
                     /gene="rapZ"
                     /locus_tag="PKH42_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOA19401.1"
                     /translation="MELVIITGMSGAGKSQAIKVLEDINYYCMDNLPPALLPNFAELC
                     KSSSMDVNKVAVVADIRGGIFFKDLFNSLKELKNKGIEYRILFLDASDDDLIKRFKEQ
                     RRPHPLSSDGTIIEGILEERNRLEEVKKKSDYIIDTTSMTLGRLKEEILNIFVKGSIS
                     HNLNITIVSFGYKYGLPKESDLVFDVRFLPNPFYIDELKNSTGNDENVKDYVMSFETT
                     SIFLEKLMDILKFLLPLYIKEGKSSLVISIGCTGGKHRSVTISNYLASMLTAENYRVL
                     LNHRDAGKN"
     gene            13032..13997
                     /gene="yvcK"
                     /locus_tag="PKH42_02870"
     CDS             13032..13997
                     /gene="yvcK"
                     /locus_tag="PKH42_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOA19402.1"
                     /translation="MKIVVFGGGTGLSVLLRGLKNYTKDISAIVTVADNGGGSGVLRE
                     DLGMLPPGDMRNCIIALSDIEPIMIKLMQHRFSDGYLKGQSFGNLFIAAMYEIFGNYE
                     HALKEIGSIFRLSGKVLPMTLQDAQLKAYLANGDCILGEKYIPEYVSKLNTKIDKIEL
                     VPYVCKPLEETVEDIKEADLIVLGPGSLYTSVIPNILVEGIPSLLAESNATVFYVCNL
                     MTQPGETDNYNVLDHVNAIIKHSMKYMIDYVIVNDEKISEELILNYKYQGAKQVLIDE
                     KQIKELEEMNIKVICSNFVDIRKNYIRHNSDKIGQLLLNYINEKK"
     gene            14012..15097
                     /locus_tag="PKH42_02875"
     CDS             14012..15097
                     /locus_tag="PKH42_02875"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOA19403.1"
                     /translation="MDKYKSIDPNQLSIADGMIDWVRVVSKDNMVLYANKKMQEDLGE
                     NIIGKKCYDVLCRYNRCENCISSTTLEKGSIMRKHHKLGNKTYMIISSPLFDDKGEIE
                     ASVEVFRDITNEVLLSEKLQEKNKKMSDDIKFAKNMQNRMLPPKGMYNGLSIDYLYES
                     SESLSGDYFDVFEIDENNTGIYICDVVGHGVTASLLTIFVRQSLRTIAKGNINLNKIM
                     KELHKTFLSLNLDYDKYFSVFFGIYNKDSHVFKYVNAGHNSVPILISGKDIKKLMLKG
                     YPIANIFDNVDYIVEKVTLKVGDKILLYTDGITETTNSEGVQFGVDRLINTIISDGNI
                     LENIKSSVNNFSIVHKDDYAVLLAEII"
     gene            15209..16504
                     /locus_tag="PKH42_02880"
     CDS             15209..16504
                     /locus_tag="PKH42_02880"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOA19404.1"
                     /translation="MKNITLDLRGSFIDEDELKKIKPEIMAAQETLLDRKGVSSEMTG
                     WLDYMYRIDKNEYEEIKAAAKYIKENSEAFILLGIGGSYLGAKAAIEAIRGDFHNELL
                     SPKVYYAGQNLSGAYLKKLIELVKEKEVCIIVISKSGTTLETALSFRVFKELIEKKYG
                     EESKNRIFVVTDKYKGALKSISDLKGYKTFVVPDDIGGRYSVITPVGLLPMAAAGLDT
                     DKFMEGLKEGAEEYSVRDFECNISLLYTAARNILNRRNKEIEIFVNYEPSCISIAEWW
                     KQLYGESEGKEGKGIFPASLNFTTDLHSMGQFVQEGREIIIETTLLIENSEDDMVIPF
                     DLNDFDKLNYISGKTFNFVNLKAFEGTFMAHNEGKVPGIIISVPKMDEFYLAKLFYFF
                     MMSCGISGYTNKVDPFTQPGVEAYKKNMFKLLKEEEHTK"
     gene            16516..17415
                     /locus_tag="PKH42_02885"
     CDS             16516..17415
                     /locus_tag="PKH42_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOA19405.1"
                     /translation="MIKLGEMQELEVAKIVNIGAYLQSEDKEKTEDRVLLPIKQVPAG
                     TKVGDKINVFVYRDSDDKIIATVKKPKITLGEIATLKVVEMSRIGAFLNWGLEKDLFL
                     PFKEQIGDIRLNGEYMVGLYIDKSDRLCATMNLFKVLRTDSPYKVNDIVKGTVFSLKR
                     GLGAMVAVDNKYLGLIHEGEIIKPLHPGQSVEVRVSNIKEDGKLDLSLKDAPRLQIDK
                     DGEKILKVLIRNKGSLPLNDDSSPEEVNKILGMSKSSFKKASGRLMKRRLIIMTKNGI
                     EMLSDDEKQQSKVKKSNIRYKKK"
     gene            17475..18176
                     /gene="deoD"
                     /locus_tag="PKH42_02890"
     CDS             17475..18176
                     /gene="deoD"
                     /locus_tag="PKH42_02890"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOA19406.1"
                     /translation="MSTPHNSAVIGQIAETVLLPGDPLRAKFIAENFLEDVEQFNTVR
                     NMYGFTGSYKGKKVSVMGSGMGCASIGIYSYELIHMYGVKNLIRVGSCGAYAPDLNLY
                     DIVLAIGASTDSNYAYQYELPGIYSATASWELLSKAKKVADDNNIKAVVGNIVTTDIF
                     YHDNSENWRKWAKMGCLAVEMETYALYCNANRAGVNALTILTVSDSLEHKTETTPEQR
                     EKSFTDMIKIALEIL"
     gene            complement(18250..18450)
                     /locus_tag="PKH42_02895"
     CDS             complement(18250..18450)
                     /locus_tag="PKH42_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOA19407.1"
                     /translation="MKTGTVKWFDSAKGFGFITSDEGNDVFVHFSAINADGFKTLEEG
                     QKVTFEVVEGTRGPQAANVEII"
     gene            complement(18597..18797)
                     /locus_tag="PKH42_02900"
     CDS             complement(18597..18797)
                     /locus_tag="PKH42_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOA19408.1"
                     /translation="MKTGTVKWFDSKKGFGFISCDDGKDVFVHFSAINSDGFKTLEEG
                     QKVNFDIVDGQKGPQASNVTVA"
     gene            19013..19954
                     /locus_tag="PKH42_02905"
     CDS             19013..19954
                     /locus_tag="PKH42_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOA19409.1"
                     /translation="MIKHFLKVFFASFLLIAIILSVTALGYIFLVDKDIVIGKIKFVN
                     DEKSEVSFYESSSFDYGTSIGKAASNMKRFNILLAGLENQRTDTIIALSYDMESKTAD
                     LISLPRDTYLPRNADDDAELKKINAIYSHEGIEGLALRVQELLGIPIEKYVIIDYEGV
                     IACVDMIGGVEVNVPFHMEYTDPYDDPPLYIDIPEGVQLLNGEQSLKFLRYRKGYDNQ
                     DLGRIEAQQQFIKSAVKKTLGFQLPAVIKEAYSHIETNINVSDLLYLAGDLVGFSTDK
                     INTHILPGMETPLEGLSFYIPDEENIKNLVYSLYGLN"
     gene            19988..20995
                     /locus_tag="PKH42_02910"
     CDS             19988..20995
                     /locus_tag="PKH42_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOA19410.1"
                     /translation="MKQHFIKVFLVSLIIFSTVITTGIFTYAKFMSPEGITQKLPEDE
                     EPIAIDEEDENLTPLEKAMRDSNRINALLIGFEGARSDTIMLGSFDRKTKEANIISIP
                     RDTYYKRDAYAKYSEMQKINAVYGSEDDGYKALLKAVEEITGISVDKYVAVNYNGVRA
                     AVDAIGGVEVNVPIHMKYTDIYDKPPLYIDIKPGPQIIYGDKAMELLRFRTGDPGYPS
                     FPNGDVGRVEMQQQFIKSAIKKSLSLRLPNVISAVYPHVETNFTLTELLGLAKDAIGF
                     STDNLTTQILPGNPKILGSNPPLSFYIPDGQEITKVVYDLYDVPLSASAEEENVAHAS
                     E"
     gene            21013..21981
                     /locus_tag="PKH42_02915"
     CDS             21013..21981
                     /locus_tag="PKH42_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOA19411.1"
                     /translation="MIKPKPLKTGDKVAVIAPSSATDLKSVKNGERKIRALGLEPVMF
                     PTCYTNYGHLSASDEERAKDVNDAFKDDTIKGIICLRGGYGATRILDLLDYETIASNP
                     KIFVGFSDITALHTAFNQICNMVTFHGPMAATRFANVKNDKVEFDVYSCESLVKNIFT
                     DIPIGLHKNPNNEELKSLNTGKAKGKLAGGNLTLLTATLGSKYEIDTKGKILFIEEIG
                     EPVYKLDRMLTTLALAGKFNECAGIILGTFIKCEREKKPYEGGLDLTLEEVVENTLVK
                     YKKPIIYNFKAGHNFPQPTIALGTTVYIDAELKQVEFLESGTSANQ"
     gene            22084..23277
                     /locus_tag="PKH42_02920"
     CDS             22084..23277
                     /locus_tag="PKH42_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOA19412.1"
                     /translation="MDIYKRIDEIFDELVQIRRDFHMHPELSQYEFRTQRKIREYLNK
                     WGIENYTCAETGVVGIIRGKNKGKTVGIRGDIDALPIHEENDVPYRSLTPGVMHACGH
                     DAHAAIVLGTAKIIKELADSEDSINGNVKIFFQPAEETIGGAERMIREGCMEEPKVDY
                     VLGLHVQPYLNAGKVELKYGKLNASTDSINIVLKGKAAHAAYPDKGIDAIVMAGHVIT
                     ALQSIVSRNTSPLNPVAISLGRISGGVKDNIIADEVKITGTLRTLDDETRQYTKDRIS
                     KIVKNTARAFGGEGLTTFCEGYKALINNNEVVDIIKENAIKLLGKENVEFKEFPSLGA
                     EDFSYFMDVAKGAFFHLGCGNPAKGITSPIHTEHFDIDEECLKVGVRLQVENVMTLLK
                     RHENM"
     gene            23321..24115
                     /locus_tag="PKH42_02925"
     CDS             23321..24115
                     /locus_tag="PKH42_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOA19413.1"
                     /translation="MKVFLSVDIEGITGVTSWNETNLGNQEHRLCAEQMTKETLAACE
                     GAITMGVKEILIKDAHDSARNIDFTKIPKCAKISRGWTNTPESMVAGLDETFDAAIFI
                     GYHSGAGYDGNPLSHTMSLSNNYIKINGEIASEFVINSYLAAKYGVPVVFLSGDKMLC
                     ETAKKFNKGIETLAVKEGVGGASISINPELSCELIREGVKKALKHTSACKIEIPDKFE
                     VEINYREHFKAKKASYFPGVSQTGPYTVKYTAKDINEFSTTMMFIL"
     gene            24134..24619
                     /locus_tag="PKH42_02930"
     CDS             24134..24619
                     /locus_tag="PKH42_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOA19414.1"
                     /translation="MFREMRRIKQLLSMEDTNAVMDRGTCGVMACLGDDDYPYAVPVN
                     YVYYNDKIYFHSSKDGHKIDAIKKHPKVSFAVVDKDTIVSEEFTSYFRSVIVFGKARI
                     AEGEEWKEAFQALVEKYSGNMPKDDKHKIVTECTRSHIIAIDIDHMTGKEAIELVNAK
                     K"
     gene            24631..25992
                     /gene="rlmD"
                     /locus_tag="PKH42_02935"
     CDS             24631..25992
                     /gene="rlmD"
                     /locus_tag="PKH42_02935"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOA19415.1"
                     /translation="MLETGLVREVEIIDMNHTGQGLAKINNFVVFISGAITGDIVKIK
                     ITDKKKNYALAEIISIIKPSEFRIDSSCPHFQKCGGCQLMHMDYKEQLKFKRNRVINE
                     FRRALVNFDEEAVTNTIGMKEPFRYRNKTAFSVAKIEGEIHIGPYEQGTYNTVDIDGC
                     LIQSKAADEAVKAFKSLMEKYNIEAYDKKTKKGIIRNTIIRNNRKNEIMLIIVTRTEN
                     FKNKELLVSELSLISNIKTIIQNINNKNTNLIMGRKNITLYGSGTIIDTIDDLVFTIS
                     PETFFQINPVQTEKLYQTAIEYADIDKDSICFDIYCGIGTISLMAAKKAKYVYGVEVV
                     EQSILNARANAKKNNISNVEFYAGKAEEILPRLYKQNIKADIIIVDPPRKGCEKEVID
                     TIINMSPKRVVYVSCNPSTLARDVKLLEYGGYKLTKAQPVDQFPWSVHVETVVLMSRV
                     EGK"
BASE COUNT         9747 a         3766 c         5389 g         7053 t
ORIGIN      
        1 tattgaagat gtatactatc tgtggtaaaa tattatatat tcacataaat cgaaggagct
       61 gctatgaaac ttaaatttat acatacatct gacctgcatt tagggaaaaa attcgatatt
      121 aaaaatttca gtttaaaaga gcgcaaaaaa agaagacagg aattatggga ttcttttgat
      181 gaaattataa ggacagccaa aattgaggaa gtacaatatt tgtttattgc aggggattta
      241 acggacggag aatatataag ctataaaagt ttaaataaaa tttcttcgaa attcagctcc
      301 ataccggaca caaatgtaat aattacctgc ggtcaaagcg acccttataa tgttaacaat
      361 atgtatgaat atatagaatg gccggataac gtatacataa ttaaaaatac cgaaacagtt
      421 gagaaactga attttcccga aaacaatttg tgcatctatt caataagttg ggacaaccat
      481 gcagaaaata aaagaaccca gcccatttac gacatatcag ttgatgaaaa caagctcaac
      541 attctcatgc ttcattgcga tatcgattct agcgacagat tgccggttaa tatagacata
      601 ataaagaata agtttgatta ttgtcctctt ggaggaagac ataacttccg tcaaataacg
      661 gacaatgttg tgtattgcgg tactcctgag cctttgtctt ttgatgagga aggcgaacat
      721 gggattataa ggggaacctt ggaaaaaaat aatacacaat actcattttt accgattgca
      781 aaaagaaaat ttataaatag acaaattgaa ttaaatattt catacagctt taataaaata
      841 ttggatttga ttaaattttc cggcgatact ttttccaaca ttaaggatta tgtgcgcata
      901 gagcttacgg gtgaagtaaa tgccgatatt tcaatggatg aaattgaaaa tgaggcaaaa
      961 cagttttttt attatataga atttgaagac aattatatat atgatacaga aaacagaaat
     1021 tatgaaggca atgaattcaa tattattgaa agctataaac ttcaatttga caaaaatgac
     1081 gataatgacg ataaattaca gcagcaggca tttaaactgg gacttgaagt tctcagaaaa
     1141 gaaaaggtgg taaaataaaa tgtacataaa cagactgaat ttaagagcct ttggaaagtt
     1201 tatcaacagg aaaatacatt ttagcaataa atttaatatt atcttcggtg aaaatgaaac
     1261 aggaaaatct accatacata attttattga gttggttatg tatggatttg atgaagatac
     1321 atctcggtat aataaatata aaccttggaa cagccccgtg tacaagggaa gcattgaaat
     1381 agcggatgaa gagaaaaaac atataatttc aagagatttt ctattagata ctatacaggt
     1441 tttcaaaaaa acaaatgaaa caaaagaaaa tgaatattcg gttgagggta tcggtgttcc
     1501 gggtgagcat ttctttaata taaacagagt atcatttaac aatacagtaa gtatcagtca
     1561 gcttggaaat aaaacagaaa aggaattggc tgatgagctg aaaaataaaa taacaaattt
     1621 aagcaataca aaagatgaag atatttcaat cgacagaatt atgcaaagcc tgtatagaat
     1681 caaagaagaa gcgggaagcg aaaataatga taaaacactg ctgggtcagt attcgcttag
     1741 gttggaagag ctcagaaatg ccaaagaaag ttcaatgaat tcaaacagac aggtaatgtt
     1801 tttggcaatg gaaaagaaaa agcttcaaag taaaattcat gagctgaata tgaaaattga
     1861 agaaaaacaa aaggaaattt cagattatga actttttttg gagaagcaaa aattcctgag
     1921 agctcagcct gtaaagaagg aaatagacaa tatcaacgat gaacttaaat taattgaaga
     1981 tgatgttacg aatgtatcag cggaggatta caaagaagcg tctgaaacag aaagcagtct
     2041 ggcttcaatg aaaaatgata agcaaagatt gattgaagaa aaagaagaat gcatttcaga
     2101 gcttgaatta atagaatctg atttgtctaa ttatattccc gaagagtttg atatagataa
     2161 gttaaatatc gattatgaca tatataaggc aaataacgaa aagatagaca ctcttaaaat
     2221 gaagatgaaa actggcaggg agagcataaa ttcaattaat atagacgaaa ttaacaattt
     2281 tattaatgac tacagggaag ttgaagagct caatcataaa atagaaatta ataatgtctt
     2341 aacggatagc aaaaattatg aattgatgaa gaaattcggc aaaaaacaaa gagggataag
     2401 cctttttacg ggacttttgg gaacggcatt catatcggct gcggcagtat catgctactt
     2461 tgcttacttt tacaataatt tcaactatta ttacggcgga ataggttttt tgatagggat
     2521 cttattctat gctttttcat ggagaaaaag aaatattgca ataaacgcaa aaaaagaaat
     2581 tgaaagtatg gaatgcgagt ttgctgatta tacattatca aacaatgaat taaataaaca
     2641 aaaagatgaa atattaaaaa gaaacaattg tcaagacttt aatagtatgg ctgataaatt
     2701 tcaagagaaa agtaaggaaa aaaacattta cgaagaaaaa atcaagcttc ttaattatga
     2761 tgatattact ttcaatgaaa tttcagcaga taataaaaaa atagaagaaa ggctcaataa
     2821 caatttaaag actttaaact tatccttgtc agaagatact ataattgaaa taaatgaagc
     2881 ttataagcgt aaagacacgg taaagctcca aacagaagaa ttaaacaaaa aaatcgaaca
     2941 aatcaaccgc gatataacaa ggctcgataa ggaaataaac tttgagcaaa gacgcttgga
     3001 aatgatactt caagcaaacg gtgcggatga tgtagaagta tttaagaaag cagttgagct
     3061 gaatgaaaaa tacaaggagc ttttaaaccg cagggaatat tacgagaaaa ttttggaaaa
     3121 tataattggc agcaccgact attacgagct taaaaaaagg actttgaatg tttccgatga
     3181 agtcaaagaa attgacaagc aagatattca acttagtatt ttcaagttaa atgaagaaaa
     3241 aacaaagctg ttacaaaata taaacgatat acatcatgaa atacgagata ttgaagacaa
     3301 cacaagaagt ttagcggaaa ttgaagagga aatcagcttt tatgaagaaa aaaccgcttt
     3361 ctttaaaaat aaaatcaaag tagctgaaat tgcagcagaa aaaataacga agatatcaga
     3421 ttcaataaaa ggtgacttta tgcctcttct tagaagctca ataagcgata atttttcata
     3481 cttgacaggc gggaaatata aaagtgttga cattgatgaa aatatgaata ttacggttat
     3541 gtctgaggaa gaaaaggaca ggaaaataga gcttgaaaat ttaagcggag gtaccttgga
     3601 ccagctttat ttttcactgc gtatagccct aagcaatatt ttaagcggca accagaatat
     3661 cccgctgatt ttggatgaca gctttattca atatgattca aaaaggctta gaaagtcatt
     3721 agaaatgctt tcaagagaaa gtgagagaag acaggtaatt ctctttacat gccaggaaag
     3781 agaagcagag ctttcaaagc aaatgaatat aaaattcaac tattttaaac tataaggata
     3841 attatgagaa tttttgctat cgctgattta cattttgact ttaaaaaaga aaagccaatg
     3901 gatgtatttg gcgacaattg ggaaaaccat gaggaaaaga taattgaaaa ctggaaaaat
     3961 accgtagatg aagaagattt ggttctaatt cccggtgata tatcatgggc tttaaagtta
     4021 aatgatgcct tagaggattt aatcagaatc gagaatttac ccggcatgaa aattataggt
     4081 aagggaaacc atgattattg gtggtcaact atctcccaac tagacagact gaatttgaaa
     4141 actataaagt ttttaaaaaa caattttcat aaatacgatg atgttgttat ttgcggtacc
     4201 agaggttggg atacaatgga agagtattgt gcggaagttg acaatgaaaa aatattttta
     4261 agagaattga acagatttaa aatttccctt caagcagcaa aaaaggtcag cggcaaaaaa
     4321 ataattatgc ttcattatcc tccctttaac aataacggac ttcctaacga atttttttca
     4381 ctgatgaaag actataatgc agatatttgc gtttacggcc atcttcacgg ggaagaaggg
     4441 catagaaatg taatagaggg tttgatagac aatattatgg tccattgtgt ttcaagcgac
     4501 tacaccggat ttaagctgaa aagaatatta tgacaagggg acgggggtgc tgtcatcaaa
     4561 tattgtatac aatagatgga caaactatct aatcggctct tgacataaaa acataacaag
     4621 agtattattt ggataaagta ataagattga agcggaaaat tgtactgaac aaagaataaa
     4681 gatgggagga aacagaatgt ttaaagcaag ggaatactat tccgacatat tggacgaaat
     4741 taaaaaaagc ggtctttgga aggaagaaag aattataacc acacctcaaa gaagtattat
     4801 cagcacaacg aaagcaggcg gagtactgaa tatgtgtgcc aataactatt taggattggc
     4861 ggacaatgag gaaataatcg aggcagcgaa gaatgcatat gacaaatggg gttacggact
     4921 ttcttcagtt agattcattt gtggcaccca agaaatacat aaagagcttg aaaaaaagat
     4981 aagcgaattt cttaaaaccg aagataccat tttgtattca tcatgttttg atgcaaacgg
     5041 cggcttgttt gaaacaataa ccaccgaaaa tgatgccata ataagcgacg agctgaacca
     5101 tgcaagcatt attgacggag taagattatc gaaagctaaa agatacagat ataataacaa
     5161 tgatatggaa tcattggagg aacagctgaa acaggctgac aaggatggag ccaatattaa
     5221 attgatagca acagacggtg tattctcaat ggacggtatt attgcaaatt tagagggtat
     5281 atgcgattta gctgataaat ataatgcact tgtaatggtt gacgacagcc atgcagtggg
     5341 atttgtaggt aaaacgggaa gaggaactcc tgaatacaga aatgtaatgg gcagagtcga
     5401 tattattacg ggaaccttag gaaaggctct tggcggtgcc agcggcggat acacaagcgg
     5461 cagaaaggaa ataatagatt tattgagaca gcgctcaaga ccatatttgt tttcaaatac
     5521 attggcgccg gctattgtcg gagcatcact caaggttctt gaaatgctga tggaagacaa
     5581 taaatataag gctaagctgg atgaaaatac gaaatatttc agagagggaa tcaaaaaaat
     5641 aggtcttaat attatagaag gcgagcaccc gatagtgccc ataatgcttg gagatgcggt
     5701 cttatcccaa aaaatggctg aaaagctgtt agaaaagggt gtttatgtag taggttttta
     5761 cttcccggtt gtgccaaagg ggaaagcaag aataagaact caaatatccg cagcccattc
     5821 aaaggaagat ttagactttg cattgaatgc attcaaagac gtaaaagaag aattaggnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt ttaattgtat aataagatgc
     6001 cctaacccca tttattcaag gtatggggac acacctccct aatttaggga aggtctctgt
     6061 ttgctggagg aatgtcccca aattaggttt tcagaaaaat ggcggtaggt cattcgcaac
     6121 gaattcccaa aatatgcgac cggagggagc tgatattttg gtgaatgaga ctgcgtattc
     6181 gttgtgttct attacattgt ttttacagtg tttaacaatt ttctacattt ctcgggaaat
     6241 aatcctaaaa tgtgagttga ttctatattt tataaagggg acgaaatgag tgataaaggg
     6301 ttgaaggaag taaaaaagac aagtataggc ggtcaggcgc ttattgaagg gatcatgatg
     6361 aagggacctt caaaaatagc tacagccgta agaaaacctg acggagaaat agtaattaaa
     6421 gagagtaata taaaacaaat atttaaacat aaatttttta aattaccgct ggtaagagga
     6481 agctttgtgc tcatagattc actggtaaac ggtgttaagg aattaatgta ttcggctgaa
     6541 ttctatggtg aagattatga agaagatgcc cttgacaggt ttttgaagaa agtatttaag
     6601 gataaggcag acacggcaat tatttatact tcggtaataa tagccttagt attttcgatt
     6661 gcaatattta tattgggtcc aaccatttta acaaatttgt taaaaaacat tattaagaat
     6721 tcctttttgc ttaacctggt ggaaggcatc gtaagagtat ccttgtttgt aggttatgtt
     6781 tatttggttt caaaacttga agatataaaa agggtattta tgtatcacgg ggctgaacat
     6841 aaaaccatat actgttatga atacggcgaa gaattgactg ttgaaaatgt ccaaagatat
     6901 tcaacacttc accctcgttg cggaacaagt tttttgataa atgtattgct gataagcatt
     6961 atagtatttt catttttcgg ttggcctaat ccgcttctga gacttgtgat aagacttgcg
     7021 atgcttccgg ttattgcagg actttcctat gaattaaaca gatatgtggg aagaagctca
     7081 agcgataata tttttacaaa aattattgct tatcccggat ttttgattca aaaaattacc
     7141 actaaggagc cggatgattc aatgattgaa gttgcaatag cagcaatgaa gagagtaatt
     7201 ccccaaggcg gagaagatga tagatggtaa caattgaaaa attattgaaa gacggtattg
     7261 atataataaa acaaagggaa tttaattctc caaagcttga agttgagctt attttatgtt
     7321 atcttttaca aaaggacaga atttatcttc atcttaacag aaaggaagaa gtaagcgaag
     7381 aaattaaaga taagttttat aaattggctg aaaagaggaa taatggttac ccgctgcaat
     7441 atataactaa ttctcaggaa tttatgggct tggactttta cgttcaggag ggtgtcctga
     7501 taccgagacc cgatactgaa acattggtag aaacggtaat aaatatagta aaagaaagat
     7561 ataataaaga gattaaaata cttgatttgg gaacaggcag cggagcaatt gcattaagcc
     7621 ttgcttatta tttaaggaac tcgtttgtta ccgcaataga cataagcgat atagctgttg
     7681 aaacgacaaa aataaatata aaaaaacaca atctaaataa tataaaggtt ataaaaggaa
     7741 atatttttga agatattttg ggtgaaaaat ttgatgttct tgtatcaaat cctccatata
     7801 tcgaaagtga tgaaattaaa aatcttcaga cagaggtttc ggtctatgag ccaaggttag
     7861 ctttggacgg aggagttgac gggttggatt attacaaacc tattgttgaa ttatttaaaa
     7921 aaatttgtga ggagaatgga gtattagctg tggaaatagg aagacagcaa aaaaaatcag
     7981 ttgaagaaat ctttctgaat tctaaagtgt ttaaagaagt taaagcatac cgggacttag
     8041 ccggaaatga cagggtaata ataggtatga cttagcatcg cttattaatt atggagacac
     8101 accgaagaat ctcatgagat ccttcgctgt gctcggatga cataacaaac atattagatt
     8161 tataagagag aggaataaaa tgttagataa attagaagca ttaaaagata aatatacaga
     8221 attaagcgaa aaactatctg accctgatat attaagcgat tcacagaagc tgcaaaagct
     8281 tatgaaagag caggcgcaga tagaacctat tgtgcttaaa tataatgaat ataaggatat
     8341 aagaaaccag cttgaagaat ccaaggaaat gctgaaggaa aaattagatg atgactttaa
     8401 ggaaatggta aaggaagaaa ttaaggaatt atcttctaaa gtagaagagc tggaaaatga
     8461 aataaaaatt ctgcttctgc cgactgaccc gaacgatgaa aaggatgtaa ttgttgagat
     8521 aagagcagga gtgggcggtg acgaagccgg attatttgca ggcgatttac ttcgcatgta
     8581 tttaagatat gcagaaagaa gaggatggaa gactgaattc atttcagtaa atgaacaagg
     8641 aataggcggt tacaaggaag taattttctc tattaaagga aaaggagcat actcaaggct
     8701 taaatatgaa agcggtgttc acagggtgca gagagttccc gatacagaat ccagcggcag
     8761 aatccacacc tcatctgcaa ccgtagcagt tctgcctgaa gcagatgata ttgatatcga
     8821 aataaatcca aatgatgtaa aggttgatgt ttttcgctct tcgggaaacg gaggacagtc
     8881 tgtaaataca actgactctg ccgtaagact aacccacata ccaaccggta ttgttatttc
     8941 atgccaggac gaaaaatctc agcttaaaaa taaggataaa gctttcaagg ttttaaaggc
     9001 gagactttat gataaatatt tgcaagagca aactgatgaa ttggctcaat caagaagaag
     9061 tcaggtgggc tccggagaca gaagcgagag gataagaact tataacttcc ctcagggaag
     9121 ggtaagcgac cacaggatag gtcttacttt gtataagctt gatgcatttc ttgacggaga
     9181 tataactgag atgattgatg cacttattac ttcagaccaa gcggagaaan nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggagatataa ctgagatgat tgatgcactt
     9361 attacttcag accaagcgga gaaattaaaa aatgagatgc cctaacccta accagtccct
     9421 taaaaattgg aggttattat gaggatagat aagtatttaa aaagcgcacg cataattaaa
     9481 agaagaacca ttgccaaaga agcatgcgag cagggaagag ttcttattaa cggcaagata
     9541 gcaaagcccg gtgcggaagt agaaatcggc gatgaaatag atataatatt cggcacaaaa
     9601 ccgatgaaaa taaaggtaga aaaactatta gaacatacag tcaaagatga atacaaacaa
     9661 atgtacagca taataaatgt ttaatcagat tatcagggta tgagttaaaa ttggagagtt
     9721 tgatatctaa gcagccatgc tgctcacata agataaatat ttaaagaggt ggttatatgg
     9781 aattaaaagg gagtaagact gaacaaaatt tgatgactgc atttgcaggc gaagctcagg
     9841 ctcatgtgaa gtacacatat tatcaaagcc aagctaaaaa agacggttat gtacaaatca
     9901 gcaaatactt tgaagaaacg gcaagaaatg aaaaagagca tgcaaaatta tggtttaaac
     9961 ttcttcatga cggaatagca cacacaagcg taaacttaca ggatgcagca gacggtgaac
    10021 actacgaatg gacagaaatg tatgcagaat ttgcaaaagt ggcaagggaa gaaggattcg
    10081 ataaaattgc ctacttattt gaaggagttg cagcgataga aaaatctcat gaagaaagat
    10141 acttagcttt gttgaataat attgaaacag gcaaagtatt caaaagagaa ggggaagttg
    10201 tttggcagtg cgataactgc ggccatatcc atgttggctc ccaagccccc aaggtatgcc
    10261 cggtttgcga tcaccctcag gctcatttcc aattgtacac aaaaccctac taaaattaga
    10321 aaattgccat tcaatatggc aatttttttg ttctgataaa atataattac cattatgcca
    10381 tattaaaaat aaatctttaa aaataatcaa ttcaatgtta gaatatacat agttgatagg
    10441 gaaagggtgg aataatggaa tatcaattga taatcaacag catgctggat gaattgacac
    10501 aaagtttcag gggacttgtg ctgaaagatg agccgttaaa aaatcatact tacttcaaaa
    10561 tcggaggtcc tgcaagcata tttgcaatac ctgaagacac agaagattta aaaacagttt
    10621 taaagctgat aaagaaatat gaaatagatt attatattat aggaaacgga acaaatctct
    10681 tggtttctga cgacgggttt aaagggataa taattaaaat aggggaaaag ttcaacaagc
    10741 ttagtcgtga aggaaataaa gtaacggcag gtgcaggatt gcttttatcc accttggcaa
    10801 agtattttgc aaaggaaggc ttgggaggtt ttgaatttgc aggaggaatc cccggatgtt
    10861 tgggaggagc aattccgata aatgcaggag cttacggcgg ggaaatgaag gatgtggtaa
    10921 cgaaggtcaa atgcatggat atggacggca gaattcatga attttccaat gaagaaatgg
    10981 aatttgaata tagacgttcc aggctgtccg attcggaata cattgttctt gaagcagaga
    11041 taagtttaac acccggcaga gttgaagata taatggaaaa aatgaaggag cttaatgaaa
    11101 agaggatttc gaagcagcca ttaaatttac caagtgccgg aagcacattt aagagaccat
    11161 taaacggata tgcttccaaa ttaatagaag atgcaggact taaagggctg aaatacagag
    11221 gagcaatggt ttcagacaaa cacagcggct ttatcgttag ttatgataac gcatcttgtg
    11281 aagatgtcct ggagcttatg agaatagtaa taagtaccgt atatgacaaa tttggaataa
    11341 tattagaacc tgagataaaa atagtaggaa cgaagttgta gaagataaag taaaacatat
    11401 aaaataacaa ggaagtttta aatgaaacta agagctgatt atcacataca ctcaacatat
    11461 tcaaaaaata gacacggcaa atcatcaatt gaagaaatag cagaaagagc ctatgaactg
    11521 ggactggagg aaatagccat cgctgaccat ggtcctgctc actatttgtt tggtataaaa
    11581 agtaatttaa taagtgaagc taaaaacatt gttgtggaaa cgagaaaaaa gtatccgaat
    11641 ttgaaaatat tgtacggtgt ggaagccaat atattggatt ataaaggaac tacagacatc
    11701 aacagcgaaa tattgaagca ctgtgatata attttatgcg ggttccactt ggcagcttta
    11761 ttttcatcac caagtgactt ctggaatttt atagttaaaa atcaattagc taaaaacaat
    11821 aaaaagcttt accatgaaat ggttgaaaag aatactcagg caattattaa tgccatgaat
    11881 aaatataaaa ttaacatttt aacccatccg ggagataaaa taccggttaa cattgataaa
    11941 attgcacaaa cagctgaaaa aactgatact atgcttgaaa taaacaacag tcacgggcac
    12001 ttaaacaagg atgaaattat aagagcatca aaatacaatg tacaatttgt aataaacagc
    12061 gattcccaca ttaaggacaa tgtggggcgc tgtgacaatg cactgagagc tgtagaaggc
    12121 tcgggtattg atttaaaccg aatagttaat ttaaaaagtt aaagggggag catatggagt
    12181 tagtaattat tacaggtatg tcaggtgccg ggaaaagcca ggcaattaag gttcttgaag
    12241 atattaatta ttattgcatg gataatcttc ctcctgcact ccttccgaat tttgccgagt
    12301 tgtgcaagag ttcatcgatg gacgttaaca aagtggcagt tgttgcggac ataagaggag
    12361 gaatcttttt taaagacttg ttcaatagtt taaaagaact gaaaaataag ggaattgaat
    12421 acagaatact ttttcttgat gcctctgatg atgacttaat caaaaggttt aaggagcaga
    12481 gaagacctca tcctttgtct tcagacggta cgataattga aggaatcctg gaagaaagaa
    12541 acagattgga agaagtaaaa aagaagtctg attatataat agatacaacc agcatgacgc
    12601 ttggaaggct taaggaagaa attttaaata tttttgtgaa aggcagcata tcccataatt
    12661 taaacataac aatagtttca ttcggctata aatacggtct tcctaaggaa tcggacctgg
    12721 tttttgatgt aaggtttctg ccaaatccat tttatataga tgaattaaaa aactcaaccg
    12781 gcaatgatga aaatgttaaa gattatgtaa tgagttttga aacgacatcc atatttttgg
    12841 aaaagttaat ggacattctt aaatttcttc tgcctttgta tataaaggaa ggaaaatcaa
    12901 gtcttgtaat atcaataggg tgtaccggag gcaagcatcg ctctgttaca atatcaaatt
    12961 atttagcttc catgcttacg gctgaaaatt acagagtttt attaaatcac agagatgcgg
    13021 ggaaaaatta aatgaaaata gtagtattcg gcggagggac aggcctttct gtattgctca
    13081 gaggattaaa aaattacaca aaagacatat cagcaattgt aaccgttgca gataacggcg
    13141 ggggttccgg agttttaaga gaagacttag gcatgctgcc gcccggagat atgagaaact
    13201 gcattattgc tctttcagat attgagccca ttatgataaa attgatgcag caccgtttca
    13261 gcgacggata tttaaagggg caaagcttcg ggaatttatt tatagctgca atgtatgaaa
    13321 tattcggcaa ttacgaacat gctttaaagg aaataggaag tattttcagg ctttcgggta
    13381 aagttcttcc catgaccctg caggatgcac aattaaaagc gtatctcgct aacggcgact
    13441 gcatattggg agaaaaatat attcctgaat atgtgagcaa attaaacacg aaaatagata
    13501 aaatcgaatt ggtgccttat gtgtgcaaac ccctggaaga aacggttgag gatataaaag
    13561 aagcagatct tattgtatta ggaccgggaa gcttgtatac cagtgtaatt ccaaatattt
    13621 tagttgaagg tataccgtct cttttggctg aaagtaatgc aaccgtattt tatgtatgca
    13681 atcttatgac acagccggga gaaacagata attacaatgt tttagaccat gtaaatgcaa
    13741 taataaaaca cagcatgaaa tacatgattg attatgtaat tgtcaatgat gaaaaaatat
    13801 ccgaagagtt gattttaaat tataaatacc agggtgcaaa acaggtactt attgatgaaa
    13861 agcagataaa agaacttgaa gaaatgaata taaaggttat atgcagcaat tttgtcgata
    13921 taagaaaaaa ctatatcaga cataattcag ataaaatagg acagctcttg ttaaactata
    13981 ttaatgaaaa aaaataaaag ggggtgcttc tatggataaa tataagagta tagatccaaa
    14041 tcagctaagc atagcggatg gtatgattga ttgggtaaga gtagtcagca aagacaacat
    14101 ggttttgtat gccaacaaaa aaatgcagga agatttagga gaaaatatta tcgggaaaaa
    14161 atgttatgac gttttatgca gatataatag atgcgaaaac tgtatttcaa gcacaacctt
    14221 ggaaaagggc tccataatgc gcaaacatca taaattggga aacaaaacat atatgattat
    14281 atcctcaccc ttatttgatg ataagggtga aattgaagct tcggtagagg tattcaggga
    14341 tataacgaat gaagttttac tttcggaaaa gttacaagaa aaaaacaaaa aaatgagtga
    14401 tgatataaag tttgcaaaaa atatgcaaaa tcgcatgctt cctccaaaag gaatgtataa
    14461 cggcttgagc attgactatt tgtatgaatc atcagaatca ttaagcggag attattttga
    14521 tgtatttgaa atagatgaaa ataatacagg tatatatatt tgtgatgttg tggggcacgg
    14581 agtaacggca tctctcttga ccatttttgt aaggcaatca ttaaggacta ttgccaaagg
    14641 aaatattaat ttgaataaaa ttatgaagga gctgcataaa acctttttat cgttgaattt
    14701 ggactatgat aaatattttt cagtattttt tggaatatat aataaagatt cccatgtatt
    14761 taaatatgtc aatgcaggac ataactcggt accgatttta ataagcggta aagatattaa
    14821 aaagcttatg cttaagggat atcctattgc aaatattttt gataatgttg attacattgt
    14881 agaaaaagtt acattaaaag tcggtgataa aatactcctt tacactgacg gcataacgga
    14941 gacaacaaat agcgaagggg tgcaattcgg cgtcgataga ttgataaata caataatatc
    15001 cgatggaaat atattagaaa acataaagtc atctgttaat aatttcagca tagttcataa
    15061 agatgattat gcagtcttat tagccgaaat aatataaaca ttctgagcga tagcgaaata
    15121 tttcatgaga ttattcactg cgttcagaat gacatggtaa cagtagctat tgtatggaga
    15181 aagggaagga caattgggag gggagaaaat gaaaaatatt actcttgatt tacgaggaag
    15241 ctttattgat gaggatgaat taaaaaaaat taaaccggaa atcatggcag ctcaggagac
    15301 acttctggac agaaaaggag tcagcagcga aatgacaggc tggctcgatt atatgtacag
    15361 aattgacaaa aatgaatacg aagaaattaa agctgcagca aaatatatta aagaaaactc
    15421 tgaagctttc atacttttgg gaataggagg ctcctattta ggggcaaaag ctgcaatcga
    15481 agctataagg ggagattttc acaatgaatt gttaagtcct aaggtatatt acgcaggaca
    15541 aaatttaagt ggagcatatt tgaaaaaact tattgagctt gttaaagaaa aagaagtttg
    15601 cattattgta atttcaaagt cgggaacaac cttggaaaca gcattgtcat tcagagtctt
    15661 taaggagctg atagagaaaa aatacggcga agaatcaaaa aaccgcattt tcgtcgtaac
    15721 agataaatat aaaggtgcct tgaagagtat atctgattta aaaggttaca aaacatttgt
    15781 ggttcccgat gatataggag gaagatattc cgtaataact cctgtaggac ttcttccaat
    15841 ggcggctgca ggtcttgata cggataaatt catggaaggc ttgaaggaag gtgcggaaga
    15901 atactcggtc agagattttg aatgcaacat tagcttactt tatacagccg caagaaacat
    15961 tttaaacaga cgaaataaag aaattgaaat atttgttaac tatgaaccat cctgcatatc
    16021 aattgccgaa tggtggaagc agctttacgg cgaaagtgaa ggaaaagaag ggaagggtat
    16081 tttccctgct tcgcttaatt tcactacaga ccttcattcc atgggacaat ttgttcaaga
    16141 gggaagagaa ataattattg aaacaaccct attaattgaa aatagtgaag atgatatggt
    16201 aattcctttt gacttaaatg attttgataa attaaattat atatcgggaa aaacatttaa
    16261 ctttgtaaac ttaaaagcat ttgagggaac cttcatggct cacaatgagg gaaaagtacc
    16321 ggggattatt atcagtgttc ctaagatgga tgaattttat cttgccaagc ttttttactt
    16381 ttttatgatg agctgtggaa taagtgggta tactaataag gtagacccct ttactcagcc
    16441 gggagtggaa gcatacaaaa agaatatgtt caagcttcta aaggaagagg aacacaccaa
    16501 gtgagaggag aaattatgat aaaactggga gaaatgcagg aattagaagt tgcaaaaata
    16561 gtaaacatag gtgcctattt gcaaagtgaa gacaaggaga aaactgaaga cagagtattg
    16621 cttccaataa agcaagttcc tgcaggaaca aaagtaggag ataaaataaa tgtatttgtt
    16681 tacagagact ctgatgacaa aattatagcc actgtaaaaa aaccaaaaat aacattgggt
    16741 gaaattgcaa ccttgaaggt tgtcgagatg tcaaggatag gagcatttct taactggggt
    16801 ttggaaaaag acttattcct tccctttaag gaacagatag gcgatataag gctaaatgga
    16861 gaatacatgg tcggtcttta tattgacaag tcggacagac tttgtgcaac catgaattta
    16921 tttaaggtat tgagaacaga ctcaccttac aaggttaacg acattgtaaa gggtactgta
    16981 ttcagcttaa aaagagggct cggtgcaatg gttgcggtag acaacaagta tttaggatta
    17041 atacatgagg gagaaattat aaagcctctt catcccggac agtcagttga ggtgagggtc
    17101 agcaatataa aggaagacgg gaaactggat ttaagcctaa aggatgctcc gaggcttcaa
    17161 atagacaagg acggtgaaaa aatcctaaag gtacttatta gaaacaaggg ctccttacct
    17221 ttaaacgatg acagcagtcc tgaagaagtt aataagattt tggggatgag caaaagctcc
    17281 tttaaaaagg cttcagggcg tttaatgaaa agaagattga ttattatgac caaaaacggc
    17341 atagaaatgc tttctgatga tgaaaaacaa caatccaagg taaaaaagtc caatataagg
    17401 tacaaaaaga agtagcttag ttgacaaaag attattagct gataacctat ataaaaaaca
    17461 caaggaggaa ttatatgtct acaccacaca atagtgcagt tatagggcaa attgccgaaa
    17521 cagtcttgtt gcccggcgac cccttgaggg caaaatttat tgcagaaaac tttcttgaag
    17581 atgttgagca atttaacact gtaagaaata tgtatggttt taccggaagc tacaaaggca
    17641 aaaaagtttc tgttatggga agcggaatgg gctgtgcttc aataggaatt tattcttatg
    17701 aattaataca tatgtacgga gtcaaaaatt taattcgtgt aggctcctgc ggggcttatg
    17761 cacctgattt gaatttgtac gacatagtac ttgcaatagg agcaagcaca gattcaaatt
    17821 acgcttatca atatgagctg ccgggaattt attcggcaac agcttcatgg gagcttttat
    17881 caaaggctaa gaaggttgcg gatgataaca atataaaagc agtagtgggg aatattgtta
    17941 ctactgatat tttttatcat gataattccg agaattggag aaaatgggca aagatgggct
    18001 gtttagccgt cgaaatggaa acctacgcct tgtattgcaa tgccaacagg gcaggagtaa
    18061 atgcccttac aatattgaca gtatctgatt ccctcgagca taagactgaa actaccccgg
    18121 agcaaagaga aaagagcttt acggacatga taaaaatagc attggagata ttataacata
    18181 taatgtgcag cgagcctgcg agccggaaaa aaaagcacct attcttttat aggtgcttta
    18241 gagtatacat tagatgattt ctacgttagc agcttgaggg cctctcgttc cttcaaccac
    18301 ttcaaaggtt actttttgtc cttcttccaa agtcttgaat ccatctgcgt ttatagcgga
    18361 aaaatgaaca aacacatcat tcccttcatc gcttgtgata aagccaaaac ctttcgcaga
    18421 atcaaaccat tttactgtac ctgttttcat taaaactcct ccatgttttt taaaaataaa
    18481 taatataatg aaaaagaact acacatgtag ttcttatcac gtctccttca aatactatat
    18541 taatataagt aatgcattta tctatattaa tgtattattt cgaaaaacaa ttactactat
    18601 gctacagtaa cattgctagc ttggggtcct ttttgaccat caactatgtc aaagttaact
    18661 ttttgtcctt cttccaaagt cttgaatcca tccgagttta tagcggaaaa atgtacaaaa
    18721 acatcctttc catcatcaca ggatataaat ccaaaaccct ttttggaatc gaaccatttt
    18781 actgtacctg ttttcattta aaaaaatcct ccgatattaa aaaagatatc attattgtaa
    18841 cagttttttt tgtaaattgc aacatattta tttcttaaat aaaaaaatca tccttaatgt
    18901 ttgttataaa gttggtgata attgtgcgtg gcaaaataat ttttacaatc caattgaaaa
    18961 agaaacggta ctttgttata attatttagc gatgatttta taaaggtggg acatgattaa
    19021 acatttttta aaggtatttt ttgcgtcttt tttgcttatt gccataatat tgagtgttac
    19081 tgcattaggg tatatttttc ttgttgataa ggatattgtg atagggaaaa tcaaatttgt
    19141 aaatgatgag aagagcgagg tttctttcta tgaatcgagt tcctttgatt atggaacttc
    19201 gataggaaaa gctgcgtcta atatgaaaag attcaatatt cttttggcag gactggaaaa
    19261 tcaaagaaca gatacaataa tagctctaag ttacgatatg gaaagtaaga cagcggactt
    19321 aatttcattg ccaagagata catatcttcc aagaaatgct gatgatgatg ccgagttaaa
    19381 aaaaatcaat gcaatatatt ctcatgaagg aattgaagga cttgcgttaa gagttcagga
    19441 gctgttggga attcctattg aaaagtatgt gattattgat tatgagggag taattgcatg
    19501 cgttgatatg attggaggag ttgaggtgaa tgtccccttt catatggaat ataccgaccc
    19561 ctacgatgat cctcccttat atatagatat tcctgaaggt gtgcagcttc ttaacgggga
    19621 gcagtcttta aaatttttaa gatacagaaa gggatatgac aaccaggatt tggggagaat
    19681 agaagcacag cagcaattta ttaaatctgc cgttaaaaaa actttaggct ttcaactgcc
    19741 cgctgtgata aaagaagctt attctcacat tgaaacaaac attaatgttt cggatttgct
    19801 gtacttggca ggcgatttgg tcggattttc taccgataaa attaatacac atatattgcc
    19861 cggcatggaa acacccttgg aaggattatc cttttacata ccggatgaag aaaatataaa
    19921 gaatttggtt tattcactct acggattaaa ttaaaacact gtgtaagata ccgaaaggcg
    19981 gatttttatg aaacaacatt ttattaaagt atttctcgta tccttaataa tattttcgac
    20041 tgtgataacc acaggtattt ttacgtatgc aaaattcatg tctcctgaag gaataactca
    20101 aaaactgccg gaggatgaag aacccattgc aatcgatgaa gaagatgaaa atttaacacc
    20161 cttggaaaaa gcaatgcgtg acagtaatcg cataaatgct ttgctgatag gatttgaagg
    20221 agcaaggtca gacacgataa tgcttggaag ctttgacagg aaaaccaaag aagcaaacat
    20281 aatatcgata ccgagagata cttattataa aagagatgca tatgcaaaat attctgaaat
    20341 gcaaaaaata aatgctgtct acggcagcga agatgatgga tataaagctt tattgaaagc
    20401 tgtggaagaa ataacaggca tttccgtaga caaatatgtt gccgttaatt ataacggtgt
    20461 aagagcagct gtagatgcta tcggcggtgt tgaagtaaat gtacccatac atatgaaata
    20521 tacggatata tatgacaaac cgcctctata tatagatata aaaccgggac cgcaaattat
    20581 atacggcgat aaggcaatgg aacttctgag gttcagaaca ggggacccgg gctatccttc
    20641 tttcccgaac ggagatgtgg gaagagtgga aatgcagcag caatttatta aatcagcaat
    20701 taaaaaatca ctgagcctaa ggcttccaaa tgtaataagc gctgtttatc ctcatgtcga
    20761 aacaaatttc actttaacgg aattattggg gcttgcaaag gatgcaatcg gattttcaac
    20821 agacaacctt acgacacaaa ttttgccggg caatccaaaa atattaggca gtaatccgcc
    20881 cttatctttc tatataccgg acggtcagga aataaccaag gttgtatacg atttatatga
    20941 tgtaccgcta tctgcaagtg cagaggaaga aaatgtagct catgcttctg agtaacacag
    21001 gggggtattt aaatgataaa accaaaacca ttaaaaaccg gtgataaagt tgcagtaata
    21061 gctccttcct cagcgactga tttgaaatcc gtaaaaaacg gagaaaggaa aattagagcc
    21121 ttaggattag agcctgtaat gtttccaacc tgttacacaa attacggaca cctttctgca
    21181 tcagatgaag aaagggcgaa agatgtaaac gatgccttta aagatgatac tattaaaggc
    21241 ataatttgtt taagaggagg ttatggagct actcgaatac tggatttgtt agattacgaa
    21301 acgatagcat caaaccccaa gatttttgta ggattcagcg acattacagc tctccataca
    21361 gcttttaatc aaatatgcaa tatggttact tttcatggtc ctatggctgc tacaagattt
    21421 gcaaacgtta aaaatgataa agttgaattt gatgtttatt cctgtgaaag cctggtaaaa
    21481 aatattttta ccgatattcc cataggtttg cacaaaaatc caaacaatga agaattaaaa
    21541 agtttaaata caggcaaggc taagggaaaa ttagccggag ggaatctgac gcttttaact
    21601 gctaccttag gctcaaaata tgaaattgac acaaagggaa aaatactttt cattgaagaa
    21661 ataggagagc ctgtttacaa attggacaga atgcttacaa cactggcatt ggcaggaaag
    21721 tttaatgagt gtgcaggaat aatactggga acctttataa aatgcgaaag agaaaagaaa
    21781 ccctacgaag gagggttgga ccttactctg gaagaagttg tggaaaacac cttagttaaa
    21841 tataagaaac ctataattta taattttaag gcaggacata attttcctca gcctacaata
    21901 gctttaggga caactgttta catagatgca gaattgaaac aagtagaatt tttggaaagc
    21961 ggaacatcag caaatcagta atataagagt atggagacac acctccctat ataaggaaaa
    22021 gtctctgccg gtaggaggaa tgtccccgaa tagtatattt taaaaagaaa ttaaggagtc
    22081 aatatggata tttataaaag aattgatgaa atatttgatg aattggtgca aatacgcagg
    22141 gactttcata tgcatccgga acttagccaa tatgagttca gaacacagag gaaaattcgt
    22201 gaatacttga ataagtgggg aatagaaaat tatacttgtg cagaaacagg tgtagtaggg
    22261 ataataagag gaaaaaacaa gggaaaaacc gttggaataa gaggagatat tgatgcactg
    22321 cccatacatg aagaaaatga cgtgccatat cgctctttaa ctcccggtgt gatgcatgca
    22381 tgcggtcatg acgctcatgc ggcaatagtg cttgggacag ctaaaataat taaggaattg
    22441 gctgattccg aagactctat taacgggaat gtcaaaatat tttttcagcc ggcggaagaa
    22501 acaataggcg gtgcagaaag aatgattcgt gaaggttgca tggaagagcc aaaggttgat
    22561 tatgttttgg gactccatgt ccagccttat ttaaatgcgg gaaaggttga acttaaatac
    22621 ggcaagctaa atgcatccac agattccata aatatagtct tgaagggaaa agctgctcac
    22681 gcagcatatc ccgacaaggg aatagacgct atagtaatgg caggacatgt aataactgca
    22741 ctgcagtcaa tagtaagcag aaatacttct cctttaaatc cggtagcaat atcactcggc
    22801 agaatttcag gcggtgttaa ggataatata attgccgacg aagtcaaaat taccggtaca
    22861 ctgagaacct tagatgatga aacaagacag tataccaagg atagaatttc aaaaattgta
    22921 aaaaatacag caagagcttt tggcggagaa gggcttacaa cattttgcga aggatataaa
    22981 gctttgatta ataataatga agtggttgat attattaagg aaaatgcaat aaagcttctg
    23041 ggaaaagaaa atgttgaatt taaagaattt ccaagccttg gagcagaaga tttttcatat
    23101 tttatggatg ttgcaaaggg tgctttcttc cacctgggat gcggtaatcc tgcaaaggga
    23161 ataacatcgc ctatccatac ggagcacttt gatattgatg aagaatgttt aaaggtaggg
    23221 gtaaggctgc aggtggaaaa tgtaatgact ttgctgaaga ggcatgagaa catgtaattc
    23281 agaacgaatt gaagtatctc attaaaaaca ggagggagaa atgaaagtat ttttaagtgt
    23341 ggatatagaa ggaatcaccg gggtaacctc atggaatgaa acaaatcttg ggaatcaaga
    23401 gcacagatta tgtgcagaac aaatgaccaa ggaaacatta gctgcatgcg aaggtgcaat
    23461 aacaatggga gttaaagaaa tattgataaa ggatgcacat gactcggcaa gaaatataga
    23521 ttttaccaaa attccaaaat gtgcaaaaat aagccggggc tggacaaata cgcccgaatc
    23581 aatggtcgcg gggttagatg aaacatttga tgcagctata tttatcggct atcattcagg
    23641 ggcaggttat gacggaaatc cgctgtctca tacaatgagc ttaagcaaca attatataaa
    23701 gattaatggt gaaatagctt ccgaatttgt tattaattca tatttagcgg caaaatacgg
    23761 cgtaccggtg gtgttcttaa gcggggataa aatgctttgc gaaacagcaa aaaaatttaa
    23821 taaaggaata gaaacccttg cggttaaaga gggagtcgga ggagcttcaa taagtatcaa
    23881 tcctgaatta tcctgcgaat tgataaggga aggagtcaaa aaagctctca agcatacttc
    23941 ggcgtgcaag attgagattc cggacaaatt tgaagttgaa atcaactaca gggagcattt
    24001 caaagcaaaa aaagcatcat attttcccgg agtttcacaa acaggaccct atacggttaa
    24061 atacacggcc aaggatataa atgaattttc aacaacaatg atgtttattc tataaaaatc
    24121 agggaggata aaaatgttca gagaaatgag aagaatcaaa caattactgt caatggaaga
    24181 cacgaatgcc gtaatggaca gaggtacatg cggggtgatg gcatgcttag gagatgacga
    24241 ttacccttat gcagtacctg taaattatgt ttattacaac gataaaattt attttcactc
    24301 ttcaaaggac gggcataaaa tagatgctat aaaaaaacat ccaaaggtat ccttcgcggt
    24361 agtagataaa gatacaatag taagcgaaga attcacttca tactttagaa gtgttattgt
    24421 atttggaaaa gcaagaattg cggaaggcga agagtggaag gaagcctttc aggctttggt
    24481 tgaaaagtat tccggaaata tgccgaaaga tgataaacat aagatagtaa cagaatgcac
    24541 aaggtcccac attattgcaa tagatatcga ccatatgaca ggcaaagaag ctatcgaatt
    24601 agtcaatgcc aaaaagtaac cggagaaatt atgcttgaaa caggattagt cagagaagtt
    24661 gaaattatcg atatgaatca cacaggccaa ggccttgcta aaataaataa ttttgtcgtg
    24721 tttataagcg gtgcaataac aggcgatata gtgaaaataa aaataacaga caaaaagaaa
    24781 aactatgcct tagcagagat aatcagcatt attaagcctt cggaattcag aatcgattca
    24841 tcatgcccgc attttcaaaa atgcggagga tgtcagctca tgcatatgga ttacaaggag
    24901 cagttaaaat tcaaacgaaa cagagtaatc aacgaattta gaagggctct ggtaaatttt
    24961 gatgaagagg ctgtaactaa taccattggc atgaaggagc catttagata cagaaataaa
    25021 acagcattct cggttgcaaa aatagagggc gaaattcata taggtcccta tgaacaaggt
    25081 acatataata ccgttgatat tgacggctgc ttaatacaaa gcaaagctgc cgatgaagca
    25141 gtaaaagctt ttaaaagctt gatggaaaag tacaacattg aagcttatga taaaaaaaca
    25201 aaaaaaggaa ttataagaaa cactataatc agaaataatc gaaaaaatga aataatgctc
    25261 attatagtta caaggaccga aaactttaaa aacaaagagc tactggttag tgaattatct
    25321 cttatatcaa acattaaaac aataattcaa aacattaaca ataaaaacac aaatttaata
    25381 atggggagaa aaaatattac tttgtacggg tcaggtacaa taattgatac tattgacgat
    25441 ttagttttca ccatttcacc cgaaacattt tttcaaatta atcccgttca gacggaaaag
    25501 ctctatcaaa cagcaattga atatgcagat attgataaag acagtatttg ttttgatatt
    25561 tattgcggca tcggaaccat atctttaatg gctgcaaaaa aagcaaaata tgtatatgga
    25621 gttgaagttg ttgagcagtc aattttaaac gcaagagcaa atgccaagaa aaacaatata
    25681 tcaaatgtgg aattttatgc aggaaaagca gaagaaatac tccctaggct atataagcaa
    25741 aatataaaag cagatataat aattgtagac cctccaagaa aaggctgtga aaaagaagta
    25801 atcgacacta taatcaacat gtcgccaaaa agagttgtat atgtttcctg caatccatca
    25861 acattggcaa gggatgtcaa attgttggaa tatggcggat acaaactaac aaaagcacag
    25921 cctgtagacc aattcccatg gtccgtccat gtggagacgg tagtattgat gtcaagggtc
    25981 gaaggaaaat aggcgcgaaa gcccagtaat actgcggttt tcaggcaatc ggacaaattt
    26041 ggcatcgagt agaccaaacg cttctcggta acttatccaa gggcaaacag gctcgaaaag
    26101 tgtgagagtt gagttgccac gatagatgtc attatgttga gttgccgagt tagat
//
LOCUS       DAOPUE010000017        26051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1868_length_26051_cov_9.573665, whole genome shotgun sequence.
ACCESSION   DAOPUE010000017 DAOPUE010000000
VERSION     DAOPUE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 26051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26051
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1868_length_26051_cov_9.573665"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1397
                     /locus_tag="PKH42_02940"
     CDS             171..1397
                     /locus_tag="PKH42_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOA19416.1"
                     /translation="MINIKRKIIISVVFALLVILSAGCQQNDKDVGALNDTKIIRAEE
                     WKNQHPDIYASFMTNAEMKETKYGGSVPDDYLEEYPNLKVFYDGYGFSKEYLGARGHI
                     YALEDVINTSRPKGGASCLACKAADFNVQLANEGEGVNKIPFNEFVEANPEMEGISCY
                     DCHRNTPGEVNITRTHLTQGLTFVEEEKSGNLACAQCHVEYYLAPDTNKVILPWHNGI
                     DTDNMIEYYDEINFRDYEHPTTGAQILKAQHPEFETFKGSIHDAQGLTCIDCHMPRIA
                     GEENLKSHQWTSPLKSKEGLTKSCIPCHGGEPEDIISKVEGVQESVYKKTNEVSDELL
                     TFINRLGKAVEDGDLPDEVLEQLRGIHRNSQFMWDFVFVENSEGFHNSQKTHKNLDNA
                     RELITEGIEILEEHGK"
     gene            complement(1447..2037)
                     /locus_tag="PKH42_02945"
     CDS             complement(1447..2037)
                     /locus_tag="PKH42_02945"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HOA19417.1"
                     /translation="MGKLFVILIGAIIFMLLSQKKAYAHCDTMDGPTVKDGIKALETK
                     NINYALKWILPAYDEELKKIFELSLKVRTLSPEAEELADRYFLESLVRIHRAGEGAGF
                     EGLKPYGEPIDEKVQAADKSIEIGNLSPLKGLVTDDEMHELEKLFEKVMALKDFEVND
                     VGAGRKYIEAYVSFFKFAEGEEHGHGHHTAHGHHIH"
     gene            complement(2215..3660)
                     /gene="proS"
                     /locus_tag="PKH42_02950"
     CDS             complement(2215..3660)
                     /gene="proS"
                     /locus_tag="PKH42_02950"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOA19418.1"
                     /translation="MAKKEKEFVKEITPMEEDFSRWYTDVIMKNELVDYSPVKGFMVI
                     RPYGYALWEKIQEYADGKFKATGHKNMYFPLLIPESLLNKEKEHVEGFAPEVAWVTQG
                     GNQELTERLVIRPTSETIICHMYAKWLNSYRDLPYLYNQWCSVVRWEKTTRPFLRTSE
                     FLWQEGHTLHETAEEAQAETLQMLDIYRQVAEDLMAMPVVVGQKSEKEKFAGAYATYT
                     MEALMHDGQALQAGTSHNLGQHFAKAFEIKFQGRNGQEQYPYSTSWGISTRLIGGLIM
                     VHSDNRGLVLPPKMAPVQVVIIPIAQKKEGVIEKANELFNRLKAKGIRVELDDNTEYS
                     PGWKFNQYEMKGYPIRIEIGPRDIENNQAVAVRRDELEKVILSLDNIEDTIYNMLETM
                     QNDLFEKAKAHREANTYVVEDYEKYKRDLPNRPGFAKMMWCGERECEDKLKEETGATI
                     RCIPFEQENLGDKCHICGKPAKHMIYAARAY"
     misc_binding    complement(3729..3926)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4182..5912
                     /locus_tag="PKH42_02955"
     CDS             4182..5912
                     /locus_tag="PKH42_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOA19419.1"
                     /translation="MKKVLSLVLVVLLILSSFSFVFAEDAETVKITVIHTNDTHSRLI
                     GTNTEIGFAKIATLIKEAKEANPNTLVLDAGDTLHGMPIVNISKGENAIKVLEAAGYD
                     YMTLGNHDFNYGKERLFELRDMSQVGMLSANIVDENGEYIFTPYVIEEVGDVKVGIYG
                     LSTPDTVILTNPNNVVGLVFKDPIEVSKEMVEELEDKTDVIIALAHLGLDESSTLTSK
                     ALAQEVEGIDLIIDGHSHSMLEGGQLENNTLIVQTKNYGQNLGYVDIEILEGEVTGIT
                     ARLLAAADTADVVPDPDLQKIIEDIEAANAEVFNEVIATTGVYLDGVRENVRTKETNL
                     GNLSADAARAATGADIAFVNGGGIREDIPVGDITKGKIAAIFPFGNTIEVKKITGADL
                     KAMLEWSVSDYPAAKGAFLQVSGLEFTFDPAKEIGSKVVEILVGGEAFDEAKEYTVAI
                     NDFMSTGGDGYAMLADYDVLAIYGTYEEIIIDYLVANGTAGSEVSGRIKVMETVVEEP
                     EPVEPEPVEPAPVEPEPVEVVIYIVVPGDVLWKIAAKYNLTYQELAEYNNIANPHLIF
                     PDQVIKVPSK"
     gene            complement(6005..6844)
                     /locus_tag="PKH42_02960"
     CDS             complement(6005..6844)
                     /locus_tag="PKH42_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA19420.1"
                     /translation="MKLFSKLIGLLILASIIVYTYAREIEPVLLTIKYENIYSNYIND
                     KNDDIKIIQFSDAHISEFFDVKNLKKAVDRINSQNPHIVVFTGDLIDKYNEYNDKDNL
                     NKIWEVLKEINAPLGKYAVYGNHDYGGGAERAYKQIMEKSGFKVLLNKNEKLEEYNIN
                     IIGMDDSIFGTKDNKAIVDYLDENYFNIILSHEPDVAEYFLEYDADLFLTGHSHGGQI
                     NLPFINYTPPLAKKYVRGMYEVSNYRQTKIYVNVGLGTSQIPMRFMATPELTVITLKG
                     TSS"
     gene            complement(6957..7214)
                     /locus_tag="PKH42_02965"
     CDS             complement(6957..7214)
                     /locus_tag="PKH42_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HOA19421.1"
                     /translation="MPASAVARVKGAVEDCIGKKVKFKLKKGKSRSHFNEGVILDTYP
                     SIFTVYIEKKDYKRIVSFNYIDILTNNVEFYICDDKETRIG"
     gene            7500..8435
                     /locus_tag="PKH42_02970"
     CDS             7500..8435
                     /locus_tag="PKH42_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOA19422.1"
                     /translation="MLDVFKSIDDTLFKLEEIEDGAWINLVNPTKEELDFIENKLSIE
                     GDLLRAALDEEESARLETEEGQVLILVDTPYIEKEDEHIIYETLPLGIIITDKNIITV
                     CLKNSIVLDQFVKNSIRTFYTYKKYRFLYQILYKNAQRYLLYLRQIDKMSNYVEEKLH
                     QSMKNKELIQLLDLSKSLVYINTSLKANQIVLEKILRINIIKKYTEDEDLLEDVIIEN
                     KQAIEMATIYSGILSGTMDAFASLISNNLNIVMKLLTSITILMAIPTMFASFYGMNVA
                     NIPLANSPFGFWIIIGISILTSVFAGFILAKKDLF"
     gene            8758..9627
                     /gene="ispE"
                     /locus_tag="PKH42_02975"
     CDS             8758..9627
                     /gene="ispE"
                     /locus_tag="PKH42_02975"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOA19423.1"
                     /translation="MLRLNSYAKINLFLDIKGKLQNGYHIIKTVMQSIDIYDEITLIP
                     LNDNKIIIECSDKSIPINEKNTCYKAVLLLKKAYGINSGIQIYINKMIPAGAGMAGGS
                     SNAAAVIKGLNKMWKLNLSEQEMIDIGAQIGADVPFCLVGGTCLAEGIGDKVTDLNDF
                     QWDNILLVKPEFSISTAFVYKNLDTNYYNSNCEEYILKYITSYDYEKAAKCVANTLEK
                     VVEKYHPEINYIKKLMIDNGAISSIMTGSGSAVFALFKDNDSLNKAYEIAKKIYPSTF
                     KSKTSKYGVKFID"
     gene            9672..10217
                     /locus_tag="PKH42_02980"
     CDS             9672..10217
                     /locus_tag="PKH42_02980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOA19424.1"
                     /translation="MKKILIIILSIALITGCTNNSADIRENEVQEAVNETQETNDEAK
                     ANEPVMAPDFELESMDGSIIKLSETRDKNVIINFWYTGCQFCVVEMPDLQKLQDTYQD
                     DLLLLAINVGESKEKVEEFLDENNLSFTVLLDEDMNVAYDYGIRSFPTTIAVNKKGEV
                     IGGYIGMLTYEQMEQLYGFFE"
     gene            complement(10278..11303)
                     /gene="ltaE"
                     /locus_tag="PKH42_02985"
     CDS             complement(10278..11303)
                     /gene="ltaE"
                     /locus_tag="PKH42_02985"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOA19425.1"
                     /translation="MKFIDLRSDTVTMPTDEMRQAIANAEVGDDVYQDDPTVNKLEKI
                     AAEKVGKEAALFVPSGTFGNQLAIMTHTRRGNEVIVGKNNHIVVHEVGASAVISGVQL
                     RTLETVNGVMSPKDVLKAIRSDDIHEPETGLICVENAHGCGAVVPLSILKEIKEIAEN
                     HSIPVHMDGARIFNAAISSGVNAKEIAACCDSVMFCLSKGLAAPAGSMVAGSKEFIDR
                     ARKNRKLMGGGMRQIGILAAAGIIALEKMTDRLVEDHENAKYLAEELSKLPGVKVKRD
                     RLDIDMVFFEMGEDVIKESVLVKKFYEKNIKINGTEDGEYRFVTHIGVSKEDIDYVIN
                     CMKELIK"
     gene            complement(11354..12205)
                     /locus_tag="PKH42_02990"
     CDS             complement(11354..12205)
                     /locus_tag="PKH42_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOA19426.1"
                     /translation="MGILLKGKPVADSIVARVKDEAEELRRNNIIPKLKIIRVGERED
                     DLAYERAAVKRMKSANIDCEVLALPLNIDKETFEDEIKNVVNDNSVHGILVFRPLPKQ
                     LNEKDIRFIINPEKDIDCLNPINAAKILEGDESGFPPCTARAAMEILKYYNIDISGKE
                     ATVIGRSMVVGKPLSMMLLKENSTVTICHSKTENLPQVAKRADILIAAIGKSKMITKD
                     YIKENSTVIDVGINADDEGNITGDVNTEDCIEKAAFITPVPAGVGSVTTAVLADHVVK
                     ACRLLNK"
     gene            complement(12215..12844)
                     /locus_tag="PKH42_02995"
     CDS             complement(12215..12844)
                     /locus_tag="PKH42_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOA19427.1"
                     /translation="MNLTELSCKDFSFELSSKKPVPGGGGAAALAGSLGVALNMMVAN
                     FSKGKKKFIQYEKQHEDILKRGEKLRIDLLNLVEKDAEYFEPLSKAYSMPATTAEEKA
                     KKENQMQKCLKDACRAPLETMELVYEGIKLHEEILDISSLTIISDIGVGVQMLKAALN
                     SAYLSVLINLKYINDDQYVNNLKKRTEVLLTDGNKKADEIFEKVLITLR"
     gene            complement(12873..13481)
                     /locus_tag="PKH42_03000"
     CDS             complement(12873..13481)
                     /locus_tag="PKH42_03000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016080339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19428.1"
                     /translation="MFRQTIYETDRLYLRILKPNYFRQVLEYYKRNHNFLKEWEPKRP
                     RDFYTAAYQKSQLKYEYNMFKRNKLVRFWIFKKEDNKLIGNICLSNIVMGNFRSCYMG
                     YKLDNDEINKGYMTEAIRKVIRIMFEDFGLHRIEVNILPRNVRSLRVMEKLNFEKEGY
                     SKRYLEINGKWEDHVHFAIYQDSFEEQKCAETARKLIFESNI"
     gene            complement(13719..14381)
                     /locus_tag="PKH42_03005"
     CDS             complement(13719..14381)
                     /locus_tag="PKH42_03005"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOA19429.1"
                     /translation="MRYCNNCNVSVVGKRKLCPLCQDRLIGDKPQEEVFPKISFVYRE
                     YSSFFKIMLLVSIIIATVSVAVNILIPESGAWSFLILGGLGSVWASLINLINQRKNIP
                     KNIVYQVMTISVISLIWDLLTGWKGWSINYVIPSVCVFAMISMAIISKIRKLYIEDYI
                     LYIIIDALFGIVPIIFVIFGLLDVLYPSIICISTSIISLSTIIIFEEKSLIAEIKRRL
                     HV"
     gene            complement(14381..15667)
                     /locus_tag="PKH42_03010"
     CDS             complement(14381..15667)
                     /locus_tag="PKH42_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19430.1"
                     /translation="MKNKRISEWSKLDNAAKIFPANSKKSDTKVFRFACELFDTVEPK
                     VLQQALDITLEAFPLYLSVIKSGLFWHYFEKSHFKALVTEETLPPCSTLYDKNNKTLL
                     FRVMYYRKRISFEVYHALADGAGALQFFRRLIFHYITIKYKYALRDKSPVLNIESSKT
                     QRLDDSFKRYYKEEKILTKKKRIKAYKIKGAKIPEYRIRIIEGVIPVDEILPKVKENK
                     TSLTIFLAAIFMCAINEEIPSRLKRKPVVLSIPVNLRNFYSSQTARNFFGVINVDYNF
                     AEGSGDLEDVIEVLKKKFKENLKPEIMARRINKLSSLEHNYVLRVIPLAIKNLILRIA
                     YAIADRSYTSTLSNVGIINMPDDIKPFIYSFDIFVSTDKIQACVNSFENILRISFTSA
                     FVSTEIQRRFFKTLTDMGINVTIESNIIDEEQEVLL"
     gene            complement(15668..16585)
                     /locus_tag="PKH42_03015"
     CDS             complement(15668..16585)
                     /locus_tag="PKH42_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOA19431.1"
                     /translation="MAINKVVRAALKALSYNEYDIKENLQGYRNFVNLTRRQILKPSL
                     KTYDFQISSKNHNIPVRVYSPEANFDCPLLLFFHGGGWVAGSIESYNKICTNMARLTN
                     HKVVSVDYRLAPENPFPAGLEDCYETAKAFITNDEINDKEVTLIGDSAGGNLAAALSL
                     LARDRKEFKVDKQILIYPSTYNDYTENSPFKSVIENGTDYLLTSKRIRDYMDLYKTKE
                     DDIYSPYFAPLLSRDFTKQPKTLIITAEYDPLRDEGEEYGRKLREAGNYVEIYRIKDA
                     LHGFFALPPKFPQVKLCYDIINRFLCEVN"
     gene            16749..16907
                     /locus_tag="PKH42_03020"
     CDS             16749..16907
                     /locus_tag="PKH42_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOA19432.1"
                     /translation="MQRYVCNACGYVYDPAEGDPDNGIAPGTSFEDLPDDWVCPLCGV
                     GKDEFSPE"
     gene            complement(16967..17446)
                     /locus_tag="PKH42_03025"
     CDS             complement(16967..17446)
                     /locus_tag="PKH42_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19433.1"
                     /translation="MKKTSYIFIIIVLLIILTGCRAKIEEKIAEKVMEKAMGGADIDI
                     DGEEVTIKTEEGDVTFGSTEWPDSGISDKIPEFKKGNIVSVMNSEAYLLVIIEDVKEN
                     DFMDYYQEVKGEFTQESYETKSEDTITYMGSNENGISIVASYSKGDSTATISASKQE"
     gene            17626..18996
                     /locus_tag="PKH42_03030"
     CDS             17626..18996
                     /locus_tag="PKH42_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOA19434.1"
                     /translation="MKKLVVCILIFIMLLTGCKSNEPLSAKNPVTINLWHNYGGLLKD
                     TMDLLIDEFNDTVGREKGIIINVTSLTGSATIAEKLKMAVNDEPGAPELPDITTLYPN
                     TAYMLAEKGLLVDIESLFSKEELSLFIPEFIEEGRLPDGKIYVLPTGKSTEVMFLNTT
                     IFNKFMKETNVSYNDLYTFEGLIDIAEKYYKWTDNQTPDIDNDGKAFICYDSVFNMAQ
                     TIYKQSGDELIADGSLNLSSDAFKDVWKYCFEPAVKGYSAIYDGYGSDLIKTGRVVAS
                     IGSTAGVLFYSDIVTYEDNITEPAEFLILPYPVVDGGKKVAIQRGGGMCIIKSNEAKE
                     YAAGIFLKWFTAPEQNLKFVSSTGYVPVTQMAVEMTVVNGDDIDDKIMKLQKTTKEMA
                     EQYTFFYTPVIENFDELQENYDNNIKNYAIQCKNDYFNLLSNMNETDAFNAATENRYE
                     EFTGQR"
     gene            19002..>19963
                     /locus_tag="PKH42_03035"
     CDS             19002..>19963
                     /locus_tag="PKH42_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19435.1"
                     /translation="MAVKDRIKYTLRLRLFLFLVVLVITILLGALIILFVSGNITAGL
                     KENETFIKNEHSRILKNTVGFYDNLAAEAVAFSRSISMSIESSLRDNDLTVDDLNNNP
                     ELLKEILSDELHQIIFYLQKSKSSGAFIILDATSNSNLPDSEYSKAGIYLKNMEPNIV
                     SSSSPTIYVLRGMADIAYKNSLPLHPQWRMEFNVADAPYYNLPMDKANKDTSLSNLYY
                     WSKAFTFPKTNEKIMMCSVPLIDIEGNVFGVCGFDVSYMLFKLLNMPDNSRYERIFCL
                     ISPIENNILKTDGSLFSGGYSARSLINNNDLKISSGKRSLYIYE"
     assembly_gap    19964..20063
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20064..20960)
                     /gene="mnmA"
                     /locus_tag="PKH42_03040"
     CDS             complement(<20064..20960)
                     /gene="mnmA"
                     /locus_tag="PKH42_03040"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOA19436.1"
                     /translation="MKKKVMVAMSGGVDSSVAAVLLKDQGYDICGATLKLFSDEDVLS
                     SCSRYRTCCSLEDVEDARSVCHKLGIEHFVFNFKETFQEEVIQKFKRKYEEGSTPNPC
                     IDCNKYIKFSKLIQRAILMEKDYIATGHYARIEFDNASGRYLLKRAVDLSKDQSYVLY
                     VLTQHDLSRTLFPLGGMLKADVRKIAEERDLINARKPDSQDICFVRDGDYAGFLENQM
                     GVESKKGNFVDTEGNIIGEHKGIIHYTVGQRKGLGIALGKPVYVIDKNKELNTVTIGS
                     EEELYSKTLKATELNMIAVEKLT"
     gene            21152..22327
                     /locus_tag="PKH42_03045"
     CDS             21152..22327
                     /locus_tag="PKH42_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOA19437.1"
                     /translation="MFKISDTVSWVGKIDWELKKFHGDEYSTHKGSSYNSYLIRDEKT
                     VLIDTVWAPFSKEFVANLKKEIDLNKIDYIIANHSEIDHSGALAELMEEIPDTPIYCT
                     KNGAKILKGHHHKDWNFVEVKTGDTLDIGKNKLIFVEARMLHWPDSMMTYMSGENILF
                     SNDAFGQHYASELMYNDKVDNDELYDEALKYYANILTPFSKFVVSKIEEVVALNIPIE
                     MICPSHGIIWRDNPLQIVNKYMDWAKNYQENQIAIVYDTMWNGTRRMAEAIAEGIKSK
                     DKDVDIKLFNCAKRDKNDIITEVFKSKMILVGSSTINSGILTATAGILEMIKGMKFTN
                     KKAAAFGSYGWSGEAVKIITEQLKEAKFEVVNDGIRELWNPDEEALERCREFGRNLV"
     gene            complement(22392..23105)
                     /locus_tag="PKH42_03050"
     CDS             complement(22392..23105)
                     /locus_tag="PKH42_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOA19438.1"
                     /translation="MEKCNHCSHDCSHGYCIDNVAIFVDLPFEIKQDILDSSNHKTYK
                     KGEIIFNAGDYFDYLFIVIKGRVKLSKISAMGKEQILKILEVGDFMGELSLFKDTVMT
                     NFAEALENTEICIIRSEKIREIIMQKPEIAIKFLEKYAERIKHSEEIIEQIGLRDVEQ
                     RIANYLIAEVEKNNIKNRNNEYIINLPITKSVLSSILGTTKETLSRKLTLLQDEGLIR
                     MEGQRKIIITDIEGLRDMQ"
     gene            23416..25083
                     /gene="hcp"
                     /locus_tag="PKH42_03055"
                     /gene_synonym="priS"
     CDS             23416..25083
                     /gene="hcp"
                     /locus_tag="PKH42_03055"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOA19439.1"
                     /translation="MGAKENLMFCYQCQEAAGCKGCTKVGVCGKKPDTAGLQDLLIYV
                     SKGLSEVTTKLRNEGKEVSADVDHLITLNLFTTITNANFDNDVFNDRIKMTLNVKSNL
                     LNQLSDKENLSEAALWTADIEEFEKKANSSEVGVLATANEDVRSLRELITYGLKGLAA
                     YMKHANVLGYDDNEINAFMQSTLAKLLDDSLTVDDLVALTLETGKFGVSGMALLDKAN
                     TSTYGNPEITKVNIGVGKNPGILISGHDLGDLEQLLKQTEGTGVDVYTHSEMLPAHYY
                     PHLKKYKHLVGNYGNAWWKQKEEFELFNGPVLMTTNCIVPPNDSYKDRLFTTGSAGYP
                     GCKYIEGNTGSEKDFTEIIELAKKCAPPTEIETGEIVGGFAHEQVFALADKVVDAVKS
                     GAIKKFFVMAGCDGRAKSRNYYTEFAKALPEDTVILTAGCAKYKYNKLNLGDIGGIPR
                     VLDAGQCNDSYSLALIALKLKEVFELQDINELPLAFNIAWYEQKAVIVLLALLYLGVK
                     NIHLGPTLPAFLSPNVANVLVEKFGIAGIGTVEDDLKIFLGDSTVTA"
     gene            25138..25347
                     /locus_tag="PKH42_03060"
     CDS             25138..25347
                     /locus_tag="PKH42_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOA19440.1"
                     /translation="MAKEITKDMYIGEILQIDPSLAEILMEAGMHCLGCPSSQRESIE
                     EAAMVHGFDADELVKKLNSHINSAK"
     gene            25608..25880
                     /locus_tag="PKH42_03065"
     CDS             25608..25880
                     /locus_tag="PKH42_03065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19441.1"
                     /translation="MEFKYEKDRIYLVNQVGESIAEVTFPQISENEVNINHTYVNPAL
                     RGQGIADKLVYELASYLRKNNIKAISTCSYAIDWFEKHPEFKDVCK"
BASE COUNT         8443 a         4538 c         4343 g         8627 t
ORIGIN      
        1 agttagcatt taaaaagacc tcctttcatg caatatttat atattcttgt aaattatagc
       61 aattattgat ataatgaagc ttaagaaaaa tatgacatac tttaccttga agacgaaaat
      121 gattaagaaa taataaggat aattgtttgt aatagattaa gataaaggag gtgataaata
      181 ttaaaagaaa gattatcatt agtgtagttt ttgcattgtt ggtaatactc tcggcaggat
      241 gtcagcaaaa tgataaagat gtcggtgcac tcaatgacac aaagataata agagcagaag
      301 aatggaagaa tcaacatccg gacatttatg catcttttat gactaatgcc gaaatgaagg
      361 aaacaaaata cggtggctct gtgcctgatg actatcttga agaatatcct aatcttaagg
      421 tattttatga tggatatgga tttagcaaag aatacttagg ggcaaggggt catatttatg
      481 ctttggaaga tgtgataaac acttcaagac caaaaggagg agccagctgt ttagcctgca
      541 aagcagctga tttcaatgta cagcttgcca atgaaggtga aggggtcaac aaaattccat
      601 ttaatgaatt tgttgaagca aatcccgaaa tggaaggtat cagctgttat gactgtcaca
      661 gaaacactcc gggagaagta aacataacaa gaacgcatct tacacagggt ttaacttttg
      721 tcgaagaaga aaagtcagga aatttagctt gcgcacagtg ccatgtagaa tattatttag
      781 ctcctgatac caataaagta attttacctt ggcataacgg aattgataca gataatatga
      841 ttgaatacta tgatgaaata aacttcaggg attatgaaca tccgactaca ggtgcgcaaa
      901 tattaaaggc acagcatcct gaatttgaaa cctttaaagg aagtatacat gatgctcaag
      961 gtttaacttg tattgattgt cacatgccta gaatagctgg agaagaaaat cttaaatctc
     1021 atcaatggac cagcccgctt aaatctaaag aaggattgac taaatcttgc attccttgtc
     1081 atggcggaga acctgaagac atcataagca aggtagaagg agttcaagaa tcagtctaca
     1141 agaaaaccaa tgaggtaagt gatgagcttt taactttcat caatcgatta ggaaaagccg
     1201 ttgaagatgg agatttgcct gatgaagtat tagaacagct gagaggcatc cacagaaatt
     1261 cacaatttat gtgggacttt gtctttgtag aaaatagtga gggattccat aactctcaaa
     1321 aaacacataa aaacctggat aatgcacgtg aattgattac agaaggaata gaaattttag
     1381 aagagcatgg aaaatagaat aaaaatatca cccttttgtt aattcaatcg ggtgatgctt
     1441 tttattctag tggatatgat gtccatgcgc agtgtgatgt ccgtgaccat gttcttcgcc
     1501 ttcagcaaat ttgaagaagc ttacatatgc ttcaatatat tttcttcctg cccctacatc
     1561 atttacttca aaatctttga gcgccataac tttttcaaac agtttttcaa gttcatgcat
     1621 ttcatcatca gtcactaatc cttttaaagg tgacaggttt ccaatttcaa tacttttatc
     1681 ggctgcttga actttttcat caattggctc gccgtacggt tttaatcctt caaatcctgc
     1741 cccttctcct gctctatgta tgcgaactaa actttcaaga aaatatctgt cagcaagttc
     1801 ctctgcttca ggacttaacg ttcttacctt taaacttaat tcaaatattt tctttaattc
     1861 ttcatcatat gcaggtaaaa tccactttaa tgcatagtta atattttttg tttctagtgc
     1921 tttaatacca tccttaacag ttggaccatc cattgtgtca caatgagcat atgccttttt
     1981 ctgcgatagg agcataaata ttattgctcc gataagaatt acaaataatt tccccatgtc
     2041 aattctcctt attatttact ttattatttt atacccttat tgtacaaaac taatcgatgt
     2101 cattctttga tttatatcaa tttaataatt aaataaaaac gaaatccctc gcctctgttc
     2161 tgaatgacag tcatcctgaa gaagtgaagg atttctataa taaggttggg acatctaata
     2221 tgctcttgct gcgtaaatca tatgttttgc aggctttccg catatgtggc acttatctcc
     2281 taagttttcc tgctcgaaag gaatacatct tattgttgca ccggtttctt cttttaattt
     2341 atcttcgcat tctctttcac cgcaccacat catttttgca aatccgggac ggttgggtaa
     2401 atctctctta tacttttcat aatcttcaac tacataagtg tttgcttctc tgtgagcttt
     2461 tgctttttca aataaatcat tttgcattgt ttcaagcatg ttataaattg tatcttcaat
     2521 attatccaga gaaagaatta ctttttccaa ttcatctctt cttactgcaa ctgcttgatt
     2581 attttctata tctcttggtc ctatttcaat acgaatagga taacccttca tttcatattg
     2641 attgaatttc catccgggtg aatattctgt attgtcatca agctctactc ttattccttt
     2701 tgcttttaat ctgttgaata attcattagc tttctcgata acgccttctt tcttttgagc
     2761 tattggaatt attacaacct gcacgggtgc cattttagga ggaagtacca accccctgtt
     2821 atcagaatga accattatta gaccaccgat taatctagtg gaaattcccc acgacgtgga
     2881 atagggatat tgctcttgcc cgtttctgcc ttgaaattta atttcaaaag cttttgcaaa
     2941 atgctgccct aaattatgag atgttcccgc ctgcagagcc tgaccgtcat gcatcaatgc
     3001 ttccatagta tatgtggcat aagcaccggc aaatttttct ttttcgcttt tttgacctac
     3061 gacaacgggc atagccatta agtcttccgc cacctgtcta tatatatcaa gcatttgtaa
     3121 agtttccgct tgagcttctt ctgctgtttc atgaagggtg tgaccttcct gccacaaaaa
     3181 ttccgatgtt cttaaaaaag gtcttgttgt tttttcccac cttacaacac tgcaccactg
     3241 gttatacaaa tatggcaaat ccctgtatga attaagccat tttgcataca tatgacatat
     3301 aatggtttct gatgtaggcc ttattacaag cctttctgtc aactcctgat ttcctccctg
     3361 agtaacccat gcaacctcag gagcgaaccc ttccacatgt tctttttcct tgtttaacaa
     3421 actttcagga attaacaatg gaaaatacat atttttatga cctgtcgctt taaattttcc
     3481 atccgcatat tcttgaattt tctcccacaa agcatatccg taaggtctta taaccataaa
     3541 acctttaacc ggcgaataat caaccaattc atttttcata ataacatctg tataccacct
     3601 gctgaaatcc tcttccatag gcgtaatttc ttttacaaat tctttttctt tcttagccat
     3661 aatttctcct ttcataacac agactcataa ctccaaattc ataaccttac aaaaataaaa
     3721 ccgcctccgt aagaggcgga tatccgcggt accactctaa ttactgtttt aaaaacagca
     3781 ctcattgtat ataagggact tccctttgag tttttaccct caaatactcc aaggcgggtt
     3841 caaaatacag ggggtcgaaa atcttccacc gatgatttcc tctctagaga ccgtgtaaat
     3901 ttactattcc tcttcatcgc aaattattaa tttgtataca taatataaaa atatataaac
     3961 tttgtcaaca gatttaagat attttttatg ctaactttgt tcagtatcac gtatttacca
     4021 caccacgata taaatacgaa tgttattaat aacaatgaca ggctgattaa aagtacagtg
     4081 actctcaaat ttgcaaatta ttcaaaagtt gtcttgaaat tccagcctcc tgactgtata
     4141 atggacataa gtcttaacat aacaaaaagg aggaaagttt aatgaagaaa gtattaagtt
     4201 tagttttagt tgtattactg attctaagca gtttcagctt tgtatttgca gaagatgctg
     4261 aaaccgtaaa gattactgtt attcacacta atgataccca ttcaagatta ataggaacaa
     4321 atactgaaat aggctttgct aaaattgcaa ctcttattaa agaagcaaaa gaagcaaatc
     4381 caaacaccct ggttcttgat gcaggagata cactccacgg tatgccgata gtaaatatct
     4441 ccaaaggtga aaatgcaatt aaggtattgg aagcagcagg ttatgattac atgacattag
     4501 gaaaccatga ctttaattac ggcaaagaaa gattgtttga attaagagat atgtctcagg
     4561 taggaatgtt gagcgccaat attgttgatg aaaacggaga atatattttt actccttatg
     4621 ttattgaaga agtaggcgat gttaaggttg gtatttacgg tctttctaca cctgatacag
     4681 taattcttac aaatcctaat aatgttgttg gtttagtatt taaagatcct atagaagtat
     4741 caaaggaaat ggtagaagaa cttgaagaca aaacagatgt tataattgca ttggctcatt
     4801 tgggtcttga tgaaagttca actttaacat caaaggcttt agctcaagaa gtagaaggaa
     4861 tcgatttgat aatagacgga cacagccatt caatgttaga aggcgggcaa ttagaaaata
     4921 atacattgat tgttcaaaca aaaaattacg gtcaaaattt aggttatgtc gatatagaaa
     4981 ttttagaagg tgaagttaca ggtattacag caagacttct tgcagctgca gacactgcag
     5041 atgttgttcc cgaccctgat ttgcaaaaaa taatagaaga tattgaggct gccaacgcag
     5101 aagtatttaa tgaggttata gcaacaacag gcgtatactt agacggagta agagaaaatg
     5161 tcagaactaa ggaaacaaat ttaggaaacc tatctgctga tgcagcaagg gctgcaacag
     5221 gtgctgatat tgcctttgta aacggcggcg gcataagaga agatatacct gttggtgata
     5281 taacaaaagg taaaatagca gctatattcc catttggcaa tacaattgaa gtaaagaaaa
     5341 ttacaggtgc tgacctgaaa gcaatgcttg aatggtccgt aagcgattat cctgcagcaa
     5401 agggagcctt cctgcaagta agcggattgg aatttacatt tgaccctgct aaggaaattg
     5461 gttccaaggt tgtagaaata ttagtcggcg gagaagcatt tgatgaagct aaggaatata
     5521 cggttgctat aaatgacttt atgtcaaccg gcggagacgg atatgcaatg ctggctgatt
     5581 atgatgttct tgcaatatac ggtacctatg aagaaatcat catagattat cttgttgcca
     5641 acggtactgc aggaagtgaa gtttcaggaa gaattaaggt tatggaaact gttgttgaag
     5701 aacctgaacc tgtagagcct gagcctgttg aacctgctcc tgtagagcct gaaccggttg
     5761 aagtagtgat ttacatagtt gtaccgggag atgttctatg gaaaatagca gcaaaatata
     5821 atttaactta tcaggaattg gctgaataca acaatattgc aaatcctcat ttaattttcc
     5881 cagaccaagt aataaaggtt ccatccaagt aaaatgaaat atatgaaggc tttgttaaca
     5941 taacaaagcc ttcaattttt ttttacaaac ccttttatgt catccttagc gtaagcgaag
     6001 gatctcatga tgatgtgcct tttaaagtaa taaccgtcaa ttccggtgtt gccataaaac
     6061 gcatcggtat ttgagatgtt cccaaaccta catttacata tattttcgtc tgcctgtaat
     6121 tggaaacctc atacatccct cttacatact tttttgccaa gggcggagtg taattaataa
     6181 atggcaggtt tatttgtcct ccgtggctgt gtccggttaa aaacaaatct gcatcatatt
     6241 ccagaaaata ttccgctaca tccggctcat gacttaatat tatattgaaa taattttcat
     6301 ccaaatagtc gactatggct ttattgtctt ttgttccaaa aattgaatca tccatcccta
     6361 ttatattaat attatattct tcaagctttt catttttgtt taatagcact ttaaaaccgc
     6421 ttttctccat aatttgctta taggctcttt ctgcgccgcc gccgtaatca tggttgccat
     6481 atactgcata cttccctaat ggagcattta tttcctttaa aacttcccat attttgttta
     6541 aattgtcttt atcattgtat tcattgtatt tatctatcaa atcacccgtg aaaactacaa
     6601 tatgcgggtt ttggctgttt attctatcga ctgctttttt taaatttttt acatcaaaaa
     6661 attcagatat atgggcatct gaaaattgta ttatttttat atcgtcattt ttatcattta
     6721 tataattact gtatatattt tcatacttaa ttgttaataa aaccggttca atttctcttg
     6781 catatgtata aacaataatt gaagctaata ttaaaagacc gattaatttt gaaaataatt
     6841 tcacttaact actcctaatc atttatataa tcaaagtata acaaattttc acaaaaaaat
     6901 atagcgaaac ttcttaatta ataatttttt aataagttag tttcactatt aaaatgctat
     6961 ccgattctgg tctctttatc atcacatata taaaattcaa cattatttgt tagaatatct
     7021 atataattaa atgatacaat ccttttatag tccttctttt caatgtagac tgtaaatatt
     7081 gaaggataag tgtctaaaat tactccttca ttgaaatgag acctgctttt acctttttta
     7141 agcttaaatt ttaccttttt cccgatacag tcttcaactg ctcctttaac tcttgcaaca
     7201 gcacttgcgg gcaaataatc accttctata ctaaatttga tgaaaaacga aaaaatacca
     7261 tttatttgtt aatatctttt ccattaacat aaaaattatg tgcattatct taaaattgcc
     7321 ggtatattta acttgaaaaa catattgata tattgtatta ttataaagca gtaaaaaagt
     7381 aaaggatgtg aagtaatgga cgaggtccct aaggtcgaat ctatattaac tgaactctct
     7441 attactaata ttatatcaat ttagtaatag agaaatctat tattaaattg aggtgaaaaa
     7501 tgcttgatgt atttaaatca attgatgaca ccctattcaa gttagaagaa attgaagatg
     7561 gtgcttggat taacttggtt aatcctacaa aagaggaatt agactttatt gaaaataagc
     7621 tgtctattga aggagatctt ttaagagcag ctttggacga agaggaaagt gcccgtttag
     7681 aaacggaaga aggtcaagtt ttaatattgg ttgatacgcc ctatattgaa aaagaagatg
     7741 aacatattat ttatgaaaca ctccctcttg gaattataat aaccgataaa aatataataa
     7801 cagtatgttt aaaaaattca attgtacttg accaatttgt gaaaaacagc attcgtactt
     7861 tctacactta taaaaaatac cgttttttat atcaaatact atacaagaat gctcaaagat
     7921 atttacttta tttaaggcaa attgacaaaa tgagcaatta tgtagaggag aagctgcatc
     7981 aatcaatgaa aaacaaggag cttatacagc tcttggactt atcaaagtct ttggtatata
     8041 ttaatacttc tcttaaagca aaccaaatag tactggaaaa aatccttaga atcaatataa
     8101 taaaaaaata taccgaggat gaggatttat tggaagatgt aattattgaa aataagcaag
     8161 ccattgaaat ggccacaatt tacagcggga ttttaagcgg tacaatggat gcatttgcat
     8221 ctcttatatc aaataattta aatattgtta tgaagctctt aacatccata acaatattaa
     8281 tggctatacc gaccatgttt gcaagctttt atggaatgaa cgttgctaat atccccctcg
     8341 caaattcgcc ttttggtttc tggataataa ttggtatttc aatactgaca tcagtatttg
     8401 caggatttat acttgcaaaa aaagatttgt tctaaggtat ggggacacac cttcaaagtc
     8461 gggtcactct atgtgggata acttttgaag gaatgtcccc aatttgaggg ggacacacaa
     8521 aaaatgaata acacccgttt ctcatcagtt acacaccaga ggacgggtgt tattcattca
     8581 tagtttcaat ttccgttttc gggggtacca ccctctttct ttatttgctt cattaaattt
     8641 atacccaata atttaaattt taatcataaa aaactattaa atttataaat aattgtattt
     8701 tttgttgttg aaaaataaat tatgttatat aatgttgaaa aaaattgaga gatggctatg
     8761 ttgagattaa actcatacgc aaaaattaat ttatttttag atattaaggg aaaacttcaa
     8821 aacggttatc atataattaa gactgttatg cagtctatcg atatttatga tgaaataacc
     8881 cttattcccc ttaatgataa taaaattatt attgaatgct ccgataaatc tattccgata
     8941 aatgaaaaga atacctgtta taaagctgtg cttttattaa agaaagctta cggtataaat
     9001 tcaggcatac aaatatatat caacaaaatg attcctgcgg gagcgggcat ggcaggaggc
     9061 agttccaatg cagctgcagt aataaaaggc ttaaataaaa tgtggaagct taatttatcg
     9121 gaacaagaaa tgatagacat tggagcacaa atcggtgctg atgtgccttt ttgcttagta
     9181 ggaggtactt gtctggcaga agggattggt gacaaagtta ctgatttaaa tgatttccaa
     9241 tgggataata ttttattggt gaagccggaa ttttcaattt cgactgcatt tgtttataaa
     9301 aaccttgata ccaattacta taattctaat tgtgaagaat acatactgaa atatattaca
     9361 tcatatgatt atgaaaaagc tgctaaatgt gttgcaaata cattagaaaa agttgtggaa
     9421 aagtatcatc ccgaaatcaa ttacattaag aaattaatga ttgacaatgg tgcaatatct
     9481 tccataatga ccggttcagg ctctgctgta tttgctcttt ttaaagacaa tgattccctg
     9541 aataaggcat acgaaattgc taaaaaaatc tatccttcta catttaagtc gaaaacatct
     9601 aagtatggag ttaaatttat agattagaca aattcgtatc tgtgtatcag ttaattaata
     9661 aggagattac catgaaaaaa atactgatta ttattttatc aatagcattg ataaccggat
     9721 gtacgaataa ttcagctgat attcgtgaaa atgaggtgca ggaagctgtc aatgaaactc
     9781 aagaaaccaa tgatgaagca aaagcaaatg agcctgtaat ggcaccggat tttgaacttg
     9841 agtctatgga cggctcaata ataaaactga gtgaaaccag agataagaat gtaattataa
     9901 atttctggta tacgggatgt caattttgcg tagtggaaat gcctgatttg caaaaacttc
     9961 aggatactta ccaagatgac cttttgttat tggcaataaa tgtaggtgag agcaaagaaa
    10021 aagttgaaga gtttttggat gaaaacaacc tgagctttac tgttttattg gatgaggata
    10081 tgaatgtagc ttatgattac ggcatccgct ccttcccaac aactattgca gtaaacaaaa
    10141 aaggtgaagt tataggcggt tatataggga tgctcactta tgagcaaatg gaacagctgt
    10201 atgggttttt tgaataggaa catctgtgcc atactggtgc caggcacctt ttaaggcgcc
    10261 tggcaccagt ggtttaatca tttaatcaat tctttcatac aatttataac ataatcaata
    10321 tcttcctttg atactcctat atgtgttaca aagcggtatt caccatcctc ggttccattt
    10381 attttaatat ttttttcata aaacttttta actagaacac tttcttttat tacatcttct
    10441 cccatttcaa aaaataccat atcaatgtca agcctatccc gttttacttt aactccgggc
    10501 agttttgaaa gttcttccgc taaatattta gcattttcat ggtcttctac aagtctgtca
    10561 gtcatttttt ccaatgcgat aattcctgct gcagccaata ttcctatttg cctcataccg
    10621 ccgcccataa gttttctgtt ctttcttgcc ctgtctataa attccttgct tcctgcaacc
    10681 attgaacctg caggtgcagc taatcccttt gataagcaga acattacgga atcacagcat
    10741 gccgcaattt cttttgcatt tactcctgat gatattgcag cattaaaaat tcttgcccca
    10801 tccatatgaa ccgggattga atgattttcc gcaatttctt ttatttcttt caatattgaa
    10861 aggggtacta ctgctccgca cccatgagca ttttcaacac atatgagacc tgtttccggc
    10921 tcgtggatat catcggatct tattgctttt aaaacatcct tgggagacat aacaccgttt
    10981 acggtttcca aagttctaag ctgtactcct gaaataacag cagacgctcc aacttcatgc
    11041 acaacgatat gattattttt ccctactatt acctcattgc ctcgtctggt atgcgtcata
    11101 attgccagtt gatttccgaa tgtaccgctt ggtacgaaca aggctgcttc tttacccacc
    11161 ttttcagcgg caatcttctc caatttatta accgtcgggt catcttggta aacatcatct
    11221 ccaacttctg catttgcaat agcctgtctc atttcatcag taggcattgt aacagtatcg
    11281 cttcttaagt ctataaattt catttttcca ccctctattt ttatttttaa taaggtatgt
    11341 taattaaccg ttattactta tttaataacc tgcatgcttt tacaacatgg tctgccagca
    11401 cggcagttgt aaccgagccc acccctgcag gtacgggagt tataaatgct gctttttcaa
    11461 tgcagtcctc cgtgttgaca tctccggtta tattcccttc atcatcggca ttgattccaa
    11521 catctattac ggttgaattt tcctttatat agtcttttgt aatcatttta gactttccaa
    11581 tggctgctat cagaatatct gctcttttgg caacttgtgg caggttttct gtttttgaat
    11641 ggcatatggt tacggttgaa ttctctttaa gaagcatcat tgacaaaggt ttccctacca
    11701 ccattgacct ccctattact gttgcctcct tgccgctgat atctatatta taatatttga
    11761 gaatttccat tgctgccctt gctgtgcatg gaggaaatcc cgattcatct ccttccaata
    11821 ttttagcagc atttatagga ttaaggcaat caatatcttt ttccgggttt attataaatc
    11881 tgatatcttt ttcatttagc tgcttgggaa gaggtctaaa taccaaaata ccgtgaacac
    11941 tgttatcatt tacaacattc tttatctcat cctcaaaagt ttccttgtct atatttaagg
    12001 gcaatgccaa aacttcacag tcaatgtttg cacttttcat tctttttaca gctgctcttt
    12061 cataagccaa atcatcttct ctttcaccga cacgaattat tttcaatttt ggaataatat
    12121 tatttcttct aagttcttcc gcttcatctt ttactctggc tacaatactg tctgccaccg
    12181 gtttgccctt taaaagtatt cccattttgc ctccttaacg caaagtaatt aaaacttttt
    12241 caaatatttc atcagccttt ttatttccgt ccgttaaaag aacttctgtt ctttttttta
    12301 aattatttac atattggtca tcatttatgt atttcaaatt aattaataca cttaaatatg
    12361 cactgttaag tgctgccttt aacatctgaa ctccaacacc tatatcacta ataattgtta
    12421 gggatgaaat atccagtatt tcttcatgaa gtttaattcc ttcataaact aactccatcg
    12481 tttctaaagg agctctgcat gcatccttaa gacatttttg catttggttt tcttttttag
    12541 ctttttcttc ggctgtagtt gcaggcatac tgtatgcttt tgaaagaggc tcgaaatatt
    12601 cagcgtcttt ttctaccaaa ttcaataaat ctatgcgaag tttttctccc cttttcaaaa
    12661 tatcttcatg ttgtttttca tattgaataa attttttttt accttttgaa aaatttgcaa
    12721 ccatcatatt gagggcaact cccaaagagc cggcaagtgc tgctgcccct ccgccaccgg
    12781 gcaccggctt ttttgatgac agttcaaaag aaaaatcttt gcatgataat tccgtcaaat
    12841 tcataaaatc acctctcata tattataacc aattatatat tactttcaaa gattaatttt
    12901 cgcgcagttt ctgcacattt ttgttcttcg aaggaatctt gatatattgc aaaatgaaca
    12961 tgatcttccc atttgccgtt gatttccaaa tatctctttg aataaccttc cttctcaaag
    13021 ttcagttttt ccataactct caatgacctt acattccgag gcagtatatt tacttcaatt
    13081 ctgtgcaggc caaagtcctc aaacataatt cgtataactt tcctgattgc ttctgtcata
    13141 tatcccttat taatttcatc attgtccaac ttgtagccca tataacagga cctaaagttt
    13201 cccataacta tattgcttaa acaaatattg cctattaatt tgttatcttc ctttttgaaa
    13261 atccaaaatc ttaccagttt gtttctttta aacatattgt attcatattt cagttggctt
    13321 ttttgatatg ctgcggtata aaaatccctt ggtctttttg gctcccattc ctttaaaaaa
    13381 ttatgattcc ttttatagta ttccaagact tgtctgaaat aattcggttt taaaattctt
    13441 aaatataatc tgtctgtttc atatattgtt tgtctaaaca tcttattttc ctttcatatg
    13501 tgtcatttaa tttcataatc cttcttatat aggcagcaga tcactttatc atgagatcct
    13561 tcgctatcgc tcaggatgac aaagcgtatt aaggcgcaac ccttgttatt gctattaata
    13621 aactacgtgt cattctgaga gctttagctc gaagaatctc atttttttaa ccatgagata
    13681 cttcgctact ctcatgatgg aaaaaatatt tagaagtatt acacatgcag ccttcttttt
    13741 atttccgcta tcaaactttt ttcttcaaaa attataattg ttgacagtga aatgatgctt
    13801 gttgatatac aaataattga cgggtacaaa acgtccagta aaccaaaaat tacaaagatt
    13861 atggggacta tcccaaacaa tgcatctatt attatatata aaatataatc ttcaatatag
    13921 agttttctga tttttgaaat tatagccatt gatatcatag caaacacgca tacggaaggt
    13981 attacataat taattgacca acctttccaa cccgttaata aatcccatat taaagatatt
    14041 acagatatgg tcataacctg gtatacaatg tttttgggaa tatttttgcg ttggtttatg
    14101 aggttaataa gacttgccca aactgagccc aatcctccca atattaaaaa tgaccacgct
    14161 ccgctttccg gtatcaatat attaaccgca actgaaacag tggcaattat tattgaaact
    14221 aagagcataa ttttgaaaaa tgaagagtat tctctataaa caaatgagat tttcggaaaa
    14281 acttcttcct ggggtttgtc gcctatcagc cggtcctgac acaaaggaca gagctttctt
    14341 tttccgacta cgcttacatt acagttgtta caatatctca ttaaagcaac acctcctgtt
    14401 cttcatcaat tatattggac tcaatagtaa cgtttattcc catatcagtt aatgttttaa
    14461 aaaaccttct ttgtatttcg gtacttacaa atgccgaagt aaaactgatt cttagtatat
    14521 tttcaaaaga gtttacgcaa gcttgtattt tgtcagtact tacaaatata tcaaatgaat
    14581 atatgaaagg tttaatatca tcaggcatat tgataatacc aacattggac aatgttgaag
    14641 tatagcttct gtctgctatt gcatatgcaa tccttaaaat cagattttta attgccagcg
    14701 gaatcactct taaaacataa ttatgctcta aggatgataa cttgttaatt cttcttgcca
    14761 taatttcggg ctttaaattt tctttaaatt ttttctttaa aacttctatt acatcttcta
    14821 aatcaccgct tccctcagca aaattatagt caacgtttat cactccaaaa aaattacggg
    14881 ctgtttgtga ggaataaaag tttctaagat ttacgggaat gcttaatact acgggctttc
    14941 tttttaatct tgatgggatt tcttcattta tggcacacat aaaaattgca gccagaaaaa
    15001 ttgtcaaaga agttttattt tccttaacct taggcaatat ttcatcaaca ggaataactc
    15061 cttcaattat tctgatacgg tattcgggta ttttcgcacc ctttatttta tatgctttaa
    15121 ttctcttttt tttagttaaa attttttctt ctttatagta tcttttaaaa ctgtcatcca
    15181 gtctctgtgt ttttgaagat tctatattta aaacaggcga tttatctctt aaggcatatt
    15241 tatatttaat agttatgtag tgaaaaatta accttctgaa aaactgcaag gctcctgctc
    15301 cgtcagctaa ggcatgatat acttcaaaac ttatgcgctt cctgtaatac ataaccctaa
    15361 acagcaatgt tttattgttt ttatcatata atgtggagca gggaggtaaa gtttcttctg
    15421 taacaagggc cttaaaatga cttttttcaa aataatgcca aaaaagcccg ctctttatta
    15481 ctgacagata aagagggaat gcttccaaag taatatcaag tgcctgttga agcaccttgg
    15541 gctctaccgt atcaaataat tcacatgcaa acctgaatac ttttgtatcg ctttttttgc
    15601 tgtttgcagg gaatatttta gctgcattat ctaatttgct ccattcggat attcttttat
    15661 ttttcaatca attcacctcg caaagaaacc ggttgattat atcatagcat aatttaacct
    15721 gaggaaattt aggaggaaga gcaaaaaatc cgtgaagggc atcttttatt ctgtatattt
    15781 caacataatt tcctgcttcc ctaagttttc tgccgtactc ttcaccttca tcacgcaagg
    15841 ggtcatattc tgcagttata attaatgtct tcggctgctt tgtaaagtcc ctggaaagca
    15901 aaggtgcaaa ataggggcta tatatatcat cttccttggt tttatacaaa tccatatagt
    15961 ctcttattct cttggaagta agcaaataat cggttccgtt ttctataacg gacttgaagg
    16021 gagaattttc cgtgtagtca ttgtatgttg acggatatat caatatttgt ttatcaacct
    16081 taaattcttt tctatccctt gcaagcaagg ataaagctgc agccaaattg cctcctgcac
    16141 tatctcctat caaagttact tctttatcat taatttcatc atttgtaata aatgcttttg
    16201 ctgtttcata acaatcctct aatcctgcgg gaaagggatt ctccggtgcc agcctgtaat
    16261 cgacggacac aaccttatgg tttgtcaacc ttgccatatt tgtacaaatt ttgttgtaac
    16321 tttctatact gcctgccacc catccgccgc catgaaaaaa taaaagcaaa ggacagtcga
    16381 aattcgcttc gggagaatat acccgaacag gaatattgtg attttttgag cttatttgaa
    16441 aatcgtacgt tttgagcgaa ggttttaaaa tttgccttcg tgttaaattt acaaaattcc
    16501 tgtagccctg caagttttct tttatatcat attcattgta ggacagagct ttaagtgcag
    16561 ctcttacaac tttattaata gccataatac cctctcgatt attttacact attatatata
    16621 taattattat atatgtctat aaaaaggtag ggactactta tcaaataaag aaatctttta
    16681 attgacattt tgataatata gtttaaaatg ttatataagt tagttacata aaattggagg
    16741 aggatactat gcaaagatat gtttgcaatg catgcggtta tgtttatgat ccggctgaag
    16801 gagatccgga taacggcata gctcccggta cttcatttga agatcttccg gatgactggg
    16861 tatgcccatt gtgcggagtt ggtaaggacg aattttcacc tgaataaagg ctaaaaaaaa
    16921 tggcgagcaa tcgccatttt tttgcttaaa gatacaagag cactttttat tcttgtttag
    16981 acgcggaaat cgttgctgtg ctatcacctt tactgtagga tgccacaata cttattccat
    17041 tttcattgct tcccatataa gttattgtat cttcactttt tgtctcataa gattcttgag
    17101 tgaattcccc cttaacttct tggtaataat ccataaagtc attttcttta acatcttcga
    17161 ttattaccag caaatatgct tcactattca tcacacttac tatattcccc tttttaaact
    17221 caggaatctt atcggaaatt ccggaatcag gccattctgt ggagccaaat gtaacatcgc
    17281 cttcttcagt ctttattgtt acctcttcgc cgtctatgtc tatatctgca cctcccatag
    17341 ccttttccat gaccttttct gcaattttct cttctatttt tgccctgcaa ccggtgagaa
    17401 ttatcaataa aacaatgatt ataaaaatat aggatgtctt tttcatattc cttccccctt
    17461 tgattttgtt attattatat agttttttat gataattgta atcaccctga gggtgatatt
    17521 tttaaaaaaa tcagtatgaa ataagggtta attaaatgga tttttacttg gaagtataat
    17581 ttaaatgtgg tataataaaa aaaatcgttt acatggagcg tcattatgaa aaaattggtt
    17641 gtatgtatat tgatttttat aatgctttta acaggatgca aaagcaatga accactaagc
    17701 gcaaaaaatc ccgtaacaat taatttatgg cataattacg gaggtctatt gaaggataca
    17761 atggaccttc taatagatga attcaatgat actgtaggaa gagaaaaggg cattattata
    17821 aatgtaactt cactcacagg ctcggcaaca atagcggaaa aattaaaaat ggcagtaaac
    17881 gacgaacccg gtgcacctga actgcctgat attacaacct tatatcccaa tactgcatat
    17941 atgttggccg aaaaaggatt gttggttgat attgaatcgc ttttttccaa agaagaattg
    18001 tcattattta ttcctgaatt tatagaagaa ggaaggcttc ccgacgggaa aatttatgtg
    18061 ctgcctacgg gtaaatctac ggaagttatg tttttaaaca caacaatatt caataaattt
    18121 atgaaagaaa caaatgtgag ctataatgac ttgtatacat tcgaaggact tatagatata
    18181 gctgaaaaat attataaatg gacagataac caaacacctg atattgataa tgacggcaaa
    18241 gccttcatat gttatgactc tgtttttaac atggctcaaa caatatacaa gcaatccggc
    18301 gatgagttga ttgcagacgg aagcttgaat ttatcttccg atgcatttaa agatgtttgg
    18361 aaatactgtt ttgagcctgc cgtgaaaggt tattcggcca tatatgacgg atatggctca
    18421 gaccttatta agaccggcag agttgttgca agcattggat ctactgccgg tgtgcttttt
    18481 tactctgata tagttacata tgaagacaat ataactgagc cggcagagtt tttgattttg
    18541 ccctatccgg ttgtagacgg cgggaaaaag gttgcaattc aacgaggcgg tggtatgtgc
    18601 attataaaat ccaatgaagc gaaagaatat gcggcaggaa tttttcttaa atggttcact
    18661 gctcccgagc aaaatctaaa atttgtatca tctacgggat atgttcccgt aacgcaaatg
    18721 gcggttgaaa tgactgttgt taatggagat gacatagacg acaaaattat gaaacttcaa
    18781 aaaaccacaa aggaaatggc tgaacaatat actttctttt atactcccgt aatagaaaat
    18841 ttcgatgaac tgcaagaaaa ttacgataat aatataaaga actatgcaat acaatgtaaa
    18901 aatgattatt tcaatttatt gagcaatatg aatgagactg atgcatttaa tgcggctact
    18961 gagaacagat atgaagaatt taccgggcag aggtgagaag tttggctgta aaagatagaa
    19021 ttaaatacac cttgcgccta agattgtttt tgtttttagt agttcttgta ataaccatat
    19081 tgttaggcgc tttgataata ctgtttgtat caggcaacat taccgcagga ttaaaagaaa
    19141 atgaaacctt tataaaaaat gaacattcac gaattttaaa aaatactgtc gggttttatg
    19201 acaatcttgc cgctgaagcc gtagcctttt cacgctctat atccatgagt atagagagca
    19261 gtttgaggga caatgacctg actgttgatg accttaataa caatccggag cttttgaagg
    19321 aaattttatc tgatgagctt catcagataa tcttttattt gcaaaaatcc aaaagcagcg
    19381 gcgcttttat aattttggat gctacatcaa acagcaatct tcccgattca gaatattcaa
    19441 aagccggaat atatttaaag aatatggagc cgaatatagt cagttcgtca agccctacaa
    19501 tttatgtatt gaggggtatg gctgatattg cttataagaa ttccctgcct cttcaccctc
    19561 aatggagaat ggaattcaat gtagcagatg caccctatta taatttgccc atggataagg
    19621 cgaacaagga tacctcttta tcaaatcttt attattggag caaagcattt acatttccaa
    19681 aaacaaatga aaaaataatg atgtgttctg ttcctctgat tgatattgaa ggaaatgttt
    19741 ttggtgtatg cggttttgat gtgagctaca tgctttttaa gcttttaaat atgcctgaca
    19801 acagtagata tgaaagaatt ttttgtttga tttccccgat tgaaaataat attttaaaaa
    19861 cagacggctc attgttttca gggggatatt cggcaaggag tttaattaac aacaatgatt
    19921 tgaaaatttc ttccggaaaa aggtctctat atatttatga aaannnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nnnnnnnnnn nnnnnnnnnn nnnggtaagt ttttcaacag ctatcatatt tagctcggta
    20101 gctttcaatg tttttgaata aagctcttct tcgcttccaa tggtaaccgt attaagttct
    20161 ttatttttat cgattacgta gaccggcttt cccaatgcaa ttccaagtcc tttgcgctgt
    20221 cccacggtat aatggataat tcctttgtgc tcaccgatta tatttccttc agtatctaca
    20281 aaattacctt tttttgattc tacacccatc tgattttcca gaaaccctgc atagtcaccg
    20341 tctctcacaa agcaaatatc ctggctgtca ggctttcttg catttattaa atctctttct
    20401 tcagctattt ttctgacatc agcttttaac attccaccca atgggaaaag tgttcttgac
    20461 aaatcatgct gggtcagcac ataaagtaca tagctttggt ccttcgacaa gtccacagcc
    20521 ctttttaata agtatctgcc gcttgcattg tcgaattcta ttctggcata atggcctgtg
    20581 gcaatataat ctttttccat taaaattgct ctttgaatca atttgctgaa ttttatatat
    20641 ttgttgcagt caatgcatgg atttggtgta ctgccctctt catattttct tttgaatttc
    20701 tgtataactt cttcttggaa tgtttcctta aaattaaaaa cgaaatgctc tattcccagt
    20761 ttgtgacata cgcttctcgc atcttccaca tcctctaaag agcagcaagt tctatacctg
    20821 ctgcaagacg ataatacgtc ttcatcgctg aacaatttca aagttgctcc gcatatatca
    20881 taaccttggt ctttaagcaa gacggcagca acagatgaat caactccccc gctcattgca
    20941 accataactt ttttcttcat aaaaactcct tttaaagtat tatacccctt cgttagttta
    21001 ttataattac agtaattcat tatataggtg taaaatatca ttgtcaataa tttagacata
    21061 accaaatatt taacataaac ttgatttaaa tcaaagaaat atggtatatt aagtaatata
    21121 gtgcatttat tattaaatca aaggaggaag tatgtttaaa ataagtgata cggtaagctg
    21181 ggttggtaaa atcgactggg aattaaaaaa atttcacggc gatgaatatt ccacccacaa
    21241 aggttcatct tataattctt atttaataag ggatgaaaaa acagtcttaa ttgacacagt
    21301 ctgggcacca ttttcaaaag aatttgttgc taatttaaaa aaggaaattg atttaaacaa
    21361 aattgactac ataatagcca accattcgga aatagaccac tcaggtgcat tagccgaatt
    21421 aatggaggaa attcccgata cacctattta ttgcaccaaa aatggagcta aaattcttaa
    21481 aggtcatcac cataaggatt ggaattttgt tgaagtaaaa acaggagata ccttggatat
    21541 cggtaaaaat aaacttatct ttgttgaagc aagaatgctt cattggccgg attcaatgat
    21601 gacatacatg tcaggagaga atattctttt cagtaatgat gcctttggcc agcactatgc
    21661 ttcagagtta atgtacaatg acaaggtaga taatgatgag ttgtatgatg aagcattaaa
    21721 gtattatgca aacatcttaa ctcccttcag caaatttgtt gtaagcaaaa tagaagaagt
    21781 tgttgcttta aacataccta ttgaaatgat ttgtcccagc catggaataa tatggagaga
    21841 taatcctctg caaatagtta acaaatatat ggattgggct aaaaactatc aggaaaatca
    21901 aattgcaatt gtttatgaca caatgtggaa cggtacaaga agaatggctg aagcaattgc
    21961 ggaaggtatt aaatctaagg acaaagatgt tgatattaaa ctgtttaact gtgcaaagcg
    22021 agataagaac gatataatta ctgaagtttt caagtcaaaa atgattcttg taggttcatc
    22081 tacaataaac agcggaattc ttaccgcaac agcgggaatt ttggaaatga taaaaggtat
    22141 gaaatttaca aataaaaaag ctgctgcatt cggcagctac ggttggagcg gcgaggcagt
    22201 aaaaattatt acggagcagc tgaaagaagc aaagtttgaa gttgtcaacg acggtatcag
    22261 ggagctttgg aatcctgatg aagaagcttt agaaagatgc agggaattcg gaagaaactt
    22321 agtataatat aattcttggt aattctgaaa gcagtgaaga atctcaaaaa tgagattctt
    22381 cattttctct tttattgcat atctcttaat ccttcaatat ccgtaattat tattttcctc
    22441 tgcccttcca tccttataag cccttcatcc tgtaaaaggg taagtttacg gctgagagtt
    22501 tctttggttg ttcccaatat ggaagataaa acacttttgg ttatcggcag gtttattata
    22561 tattcattat ttctattttt aatattgttt ttttccacct ctgcaatgag gtaatttgct
    22621 attctttgct ccacatccct taaacctatt tgctctatta tctcttccga atgtttaatt
    22681 ctttcagcat acttttcaag aaacttaatt gctatttccg gtttttgcat aataatttca
    22741 cgaatttttt cgcttcttat tatacaaatt tcagtatttt ccaatgcctc ggcaaaatta
    22801 gtcatcaccg tatccttgaa cagcgacaac tctcccataa aatctcccac ctcaagaatt
    22861 tttagtattt gctccttgcc catagcagag attttagaga gtttaaccct ccccttgata
    22921 acaataaaca aataatcaaa ataatctccg gcgttaaata taatttcgcc ttttttataa
    22981 gttttatggt tggatgaatc caagatgtct tgtttaattt caaaaggcaa gtctacaaat
    23041 atggcaacat tatctataca atacccatgg ctgcagtcgt ggctgcagtg attacatttt
    23101 tccatgccac cctcccagac tgttttaatt aatcataaca gttagcttac ttttattcaa
    23161 caaaaaagat gattaaatcc gccgcttagg ctcatgatga cataggggtt tatgctcagg
    23221 ataagataaa ggtgtgacaa taaatactta ttatttataa ttgtttatat atagacaaag
    23281 acgggtataa aaaaagagca gaaaaaattg ttctttaaaa attattgtaa caaatgttac
    23341 agaagtgatt tttaaaatat attataatcg tgtcataaaa acaaatataa gaaaattgaa
    23401 aggagaacaa tttatatggg tgcaaaagaa aatttgatgt tttgttatca atgtcaggag
    23461 gcggcaggct gtaaaggctg cactaaggta ggagtgtgcg gcaaaaaacc tgacactgcc
    23521 ggattacagg atttattaat ctatgtatca aaaggattat cggaagtaac cacaaaatta
    23581 agaaatgaag gaaaagaagt atcagccgat gttgatcatt taatcacttt aaacttattt
    23641 acgactatta caaatgccaa ctttgacaat gatgtattta atgacagaat aaaaatgact
    23701 ttaaacgtta aatcgaactt attaaatcaa ttatcagata aagaaaactt atcggaagca
    23761 gccttatgga ctgcagatat agaagagttt gaaaaaaaag caaactcaag tgaagttggt
    23821 gttttagcca ctgccaatga agatgtaaga agcttaagag agcttattac atacggatta
    23881 aaaggattag cagcatatat gaaacatgca aatgtgctcg gatatgacga caatgaaata
    23941 aatgccttta tgcagagcac attggcaaag ttgcttgatg attcattgac agttgatgat
    24001 ttggtagcat tgacattgga aacaggaaag ttcggagtta gcggaatggc attgcttgat
    24061 aaggcaaaca caagcacata cggaaatcct gaaataacaa aggttaatat aggcgtaggt
    24121 aaaaacccgg gaatcttaat ttcaggtcat gacttgggag acctggagca actgttaaaa
    24181 caaacagaag gtaccggagt tgatgtttac actcattcag aaatgctgcc ggcacactac
    24241 tatcctcatt taaagaaata caaacactta gtaggcaatt atggaaatgc ctggtggaaa
    24301 caaaaagaag aatttgagtt attcaacgga cctgttttga tgactacaaa ctgtatagtt
    24361 cctcctaatg acagctataa ggacagattg ttcacaacag gttcggcagg ttatccggga
    24421 tgtaaatata tagagggaaa tacaggctct gaaaaagatt ttactgaaat tatagaatta
    24481 gctaaaaaat gtgctccgcc gacagaaata gaaacaggtg aaatagtggg aggttttgcc
    24541 catgaacaag tatttgcctt agctgataaa gttgtagatg cggttaaatc aggagctatt
    24601 aaaaaattct ttgtaatggc aggctgtgac ggcagagcta aatcaagaaa ctattacacg
    24661 gaatttgcaa aagctttgcc cgaagatacg gttattttaa cagccggctg cgcaaaatat
    24721 aaatacaaca aattaaattt aggtgatata ggcggaatac caagggttct tgatgcaggg
    24781 cagtgtaacg attcatattc attggctctt atagctctta aattaaagga agtatttgaa
    24841 ttacaggata taaatgaatt gcccttagca tttaacattg catggtatga gcaaaaagcg
    24901 gttatagtac tcttggcgct tctatattta ggagttaaga atattcactt aggaccgact
    24961 ttacctgctt tcctttcacc aaatgtcgca aatgtattag ttgaaaaatt tggtatagcc
    25021 ggaatcggta ctgtagaaga cgacttaaag atattcttgg gagactcaac tgttactgca
    25081 taatgaattt taattaatag attggtaatg atatcaagag tacaaaggag gttttttatg
    25141 gcaaaagaaa taactaagga tatgtatata ggagaaatac tgcaaataga cccaagttta
    25201 gccgaaatat taatggaagc aggaatgcac tgcctcggat gtccttcttc acaaagagaa
    25261 tcaattgaag aagcagccat ggtgcatggt tttgatgcgg atgaactggt taaaaagtta
    25321 aattctcata taaactctgc caaataaatt ataagagagc atggacaaca tgctctcttg
    25381 tttcatgcta tgaatggttt ataaaaaatg tatccctatt aatatccaac aggaaaatta
    25441 aacattaaaa tttcaattgt aaataatatt aaaagaaagg caggtgacaa acctgattcg
    25501 aaaccgccca acaataaata aatatgttgt cgggtactcg gaaatcttgt tgtaaatgca
    25561 ataagataaa aagcataagg ataatattat gtttagaacc aggaactatg gaatttaaat
    25621 atgaaaaaga caggatatac ctagtaaacc aagtaggaga aagtattgct gaagtaactt
    25681 ttccccaaat atccgaaaat gaagttaaca taaatcatac ttatgtaaac cccgctctac
    25741 gaggtcaagg catcgcagat aaattagttt atgaattggc ttcatatctg agaaaaaata
    25801 atataaaagc aatatcaaca tgctcctatg cgatagactg gtttgaaaaa catccggaat
    25861 tcaaagatgt gtgcaaataa aaatattttt taaaaaacac tttacattta taaatatttg
    25921 tgataatgta tgtaaaatga taaatgccgt gacaaggaat agtagatata aaagtttttt
    25981 atagagagtg catggtaggt ggaaatgcat aaaaacctat attgaacaca cctttgagca
    26041 aatggatgaa a
//
LOCUS       DAOPUE010000018        25236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_1937_length_25236_cov_9.325523, whole genome shotgun sequence.
ACCESSION   DAOPUE010000018 DAOPUE010000000
VERSION     DAOPUE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 25236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25236
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1937_length_25236_cov_9.325523"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PKH42_03070"
     CDS             <1..1148
                     /locus_tag="PKH42_03070"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOA19442.1"
                     /translation="HIVDGLGGKTEGVPREDGFDITVASEVMAVFCLSNDIIELKENL
                     SKIIVAYNRAGEPVTAGQLKAHGAMAALLKDALKPNLVQTLEGTPAFIHGGPFANIAH
                     GCNSVIATRMSCKIADYAVTEAGFGADLGAEKFVDIKCRKTGLKPSAVVIVASVRALK
                     HHGGAAKADLGKENLEALDKGLPNLLKHVENITQVFKIPAVVAINKFPTDTEAELKLI
                     KEKCMELGVNVKLSEVWGKGSEGGVDLANEVIRLTEQESSLEFAYDLDISIKDKIRAI
                     ARKIYGADDVEFSSKASKEIANFEKLGFGNLPICMAKNQYSFTDDSKVFGRPTGFNIT
                     IRDITISAGAGFLVALTGDIMKMPGLPKVPAAENIDIDVDGRIKGLF"
     gene            complement(1178..2050)
                     /locus_tag="PKH42_03075"
     CDS             complement(1178..2050)
                     /locus_tag="PKH42_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA19443.1"
                     /translation="MNKTKGLTFGILAGFIYGFTPILGKLTYLEGSNPISLTFYRNLL
                     SIPFFFVMLKYNNVPLKVTKSQTKQLAILGLLASATALSLYGSYNYISVGMSTTIHYI
                     YPVLVTAACIIIFGEKISRDKVVSLILSTMGITLFFEGSINITGILIAFMSGVFFAGY
                     LLFMDKSGLNTIYQFKITFYTAAFSSMYLFIFGIISKNLVFTMTFKGWFFTVLVAVFV
                     SFFANTFVALGVKYVGPTVTSIVGMLEPITSIVMGILFINEPITLRNILACVLILLGV
                     LIVSLSKEKASEDY"
     gene            complement(2071..2568)
                     /locus_tag="PKH42_03080"
     CDS             complement(2071..2568)
                     /locus_tag="PKH42_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOA19444.1"
                     /translation="MSLYKQWTENLEAIKTQGEYNEFFKGYMEKETECYRRILENKTA
                     VIEGKIVELAEENKMSALEYIGFLDGANTSFKKELVLDDLTEESEIKAEFDFEKLLWN
                     MHDAKAEWLYGMEEWENIFDEDKRKQIKKEFNRSKIVVKGEKIGRNDPCPCGSGLKYK
                     KCCGK"
     gene            complement(2762..3187)
                     /gene="mscL"
                     /locus_tag="PKH42_03085"
     CDS             complement(2762..3187)
                     /gene="mscL"
                     /locus_tag="PKH42_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOA19445.1"
                     /translation="MWDDFKKFAFQGNVLDLAIGVIMGGAFGKIVTSVVNDLIMPVLG
                     YLMAGIDFKDMKYVMSEAIMEGDTVIKPEAAIMYGNFIQNVVDFLIVALSIFLFIKLI
                     NKSKEKLKKQEEAAPEPEAPPAPTQEELLTEIRDLLKEK"
     gene            complement(3309..6011)
                     /locus_tag="PKH42_03090"
     CDS             complement(3309..6011)
                     /locus_tag="PKH42_03090"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOA19446.1"
                     /translation="MKLVLSLFDLMFSLYRCEASVFVLHLCYNLKKKGMINMMFKRIA
                     AISAAAILLLSSSAYGSSLYTVYDLSEEIRLSKSITYERIEKYTSSGWMNINVIRADL
                     TDEHTEIKPINNEDGISNRSTLSSMIRSSGAVAAVNGDFFYMGDPTYTYGPIIRDNKL
                     ITSPLPFNDGYPTVSKLKDEIDISVWNPKITLYGSDSTEFNVVVMNKTSNIEWGPTIL
                     TSDWNKTSPGYTTQDIVEVVVVDNIVSSVRKNQPSTIIPENGYIVASSNPATMELMLN
                     SFKEGHPVSLNIELDFSPEDIDWSFGALNYLVKDGRENVISSQVLGVNPRTAIGFNKD
                     NTEIILVTVDGRHRDYVGAKQTELAKIMIELGAYNAVNMDGGGSTTMAVDFLRNANVT
                     VVNFPSDGRERKIASGVGVFNNYPDSNKVDKIEITASQNTVFNKTETNLDLKFYNEYY
                     TPVEFDLKNVDYTVSPAGAGKVVNNVFMPEKPGKATITAHAGNAEGHIEIDILDKPVA
                     LKLDTDSLTLGFNDKYELGTVLGIDKDGNSAYIPWKYITASYRNRVGKIEDGVFISSD
                     IANTGAVTLTFGDAIKHIQVKVGYRYKTLNRFENLDNLKLSLYPEESAGSISITKDFV
                     KEGKNALRLDYDFTKMTDQSIAFVEFGDEGEGIKLEDKPLAIGMWVYGDNKNHWLRTR
                     ITDAYGTMVKIDFEEEVNWTGWKWVEAKIPEGIAYPITLKNIYLAEINEARKDTGTIY
                     VDNLRILYEPKDKQLGLREETKFEDKMKTSSIKNYTEKLTITQGNYYYEGIGNIVYFE
                     GIISNGTMSSADTTMWNNIKSFMDFEDKVLVLSMNGDIDNINDKREIKILNEILEKAS
                     QNNDVFVVFKGENESTVIENKVRYITYDDSFEIGISEGKVSYKN"
     regulatory      6217..6312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6477..7655
                     /gene="metK"
                     /locus_tag="PKH42_03095"
     CDS             6477..7655
                     /gene="metK"
                     /locus_tag="PKH42_03095"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOA19447.1"
                     /translation="MRKWLFTSESVTEGHPDKMCDQIADGILDAIMEQDPNGRVACEA
                     ITKTGFVLVAGEISTSCYIDIPHLVRETIKNIGYDNALYGFDYKTCAVFTSIEEQSQD
                     IALGVNEADEYKKNNNDINDMIGAGDQGMMFGFACNETPELMPMPISLAHKLAKKLAE
                     VRKNNIVDYLRPDGKTQVTVEYHDNKPVRIDTIVISTQHNPEVNIDTIKKDLIEIVIK
                     PTVPNELLDENTKYIVNPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSGKD
                     PTKVDRSAAYAARYIAKNVVAAGLADKCEVEVAYAIGVAKPVSIMVETFGTNKVDDEI
                     IQKAITENFDLRPAAIIEKFNLRRPIYQKLAAYGHFGREDLDLPWEKTDKTDELKKYL
                     "
     gene            7706..9202
                     /gene="malQ"
                     /locus_tag="PKH42_03100"
     CDS             7706..9202
                     /gene="malQ"
                     /locus_tag="PKH42_03100"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOA19448.1"
                     /translation="MFERSTGILLPIFSLPSKYGIGTLGKEAYKFIDFLRSAGQKYWQ
                     FLPIGHTSYGDSPYSCFSVFAGNPYFIDLDVLFEEGFIEETDLKGIDFGSDDERIDYK
                     KIFETRYGILKKAYLNKGMSCDLASFTCNNPWVSDYALFMSLKYKNNQLPWYKWDEMF
                     IKRDINTLQKYKNDFYDEINFWIFLQYIFFEQYFKLKDYANSSGISIIGDIPIYVAED
                     SVEAWTEKKLLMLNEDNLPICVAGVPPDDFSDDGQLWGNPVYNWEYLKETGYMWWIDR
                     IAFSFKLFDVVRIDHFRGFDEFWAVPYGSENAVNGSWMPAYGKQMFQTLKKSLGNLNI
                     IAEDLGIITDSVIKLKNDSGYAGMKVLQFAFDGNNDNPYLPYNYEENDVAYTGTHDND
                     TLKGWFNKLTLKEKQYVLQKLNINMVNEKDDNNLVYELIKAIYKSKASLCIVPLQDFL
                     CLGSEARVNIPSTLGNNWRWRLKNSMITDELAEKIKALKTERDVQNFN"
     gene            complement(9388..11160)
                     /locus_tag="PKH42_03105"
     CDS             complement(9388..11160)
                     /locus_tag="PKH42_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOA19449.1"
                     /translation="MYKFNSQSEFYRYPQGGIKSNEIITFKILVKRNICAPELLIEKR
                     HDYSRTFHDKIKMHWISQEKNYDIYQAMLSIEEYGHYYYSFIFDNEKSKDYELLIYDD
                     KYITPNWAKGGIIYHIFVDRFFRTKIIKKKGAIIRNWGETPYYLPDKKGEILNNDFFG
                     GNLDGIREKLPYIAKLGVSIIYLSPIFEAYSNHKYDTGNYHKIDPMFGTENSLKKLIK
                     EAKKYNIHVILDGVFSHTGDDSIYFNKYGRYDSIGAYQSKDSPYYDWYTFNNWNDDYV
                     SWWGIKTLPEVNENNDSYINFITGKNGVITHWQKTGIMGWRLDVADELPDEFIIALRN
                     SIKKNNEDALIIGEVWEDASNKYSYNKLREYLCGKQLDSVTNYPLRKGIIEFVKNKDC
                     TNLNHIMNKVIETYPPDTVNCLMNVLGTHDTERILTILGSKRVPSSRSEMAESKLSYE
                     ELEQGIKFLKIAVLLQMTLPGIPCIYYGDEAGIEGWDDPFNRRCYPWGKENKEILDFY
                     LFITKLRRTNKIFAEGKYRCLIHDKEVFVFERFNENERIIIAVNLSQKEITLKFKEDM
                     IEYTNNIKNNIFNIEKENYLILLS"
     gene            complement(11238..13658)
                     /locus_tag="PKH42_03110"
     CDS             complement(11238..13658)
                     /locus_tag="PKH42_03110"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOA19450.1"
                     /translation="MKDSKIDATLINRLIDEEIIRYWNTEPEKATINQIYRAFSIVIR
                     DLLLKKRNEFKRMQNEHKQVYYICMEFLIGKSLRNNLFNLGIEDEAKEALKKYNITPE
                     ELYSIERDPGLGNGGLGRLAACFMDSLATQDYAAVGFSIKYEFGLFKQKLIDGWQTEL
                     PDDWLTTSDVWLMPRTDERVIVKFDGTIREEWTGEGLKIHHDCPTEIEAIPYDMMISG
                     YNSKGVSLLRLWESRSINSIDMNLFSQGDYIRALSEDSMAEAISKVLYPADDHNEGKI
                     LRLKQQYFLVSASAQNIVKNHMKKYNSLDNFSEKNAIHINDTHPSLIIPELMRIFMDE
                     YGLSWEKAWTIVVNTVSYTNHTVLKEALETFDNNIVQTKLPRIYMIIKEINRRFIEEA
                     SAKYNNRDKAFNMSIFGYGKVNMANLCIIGSHKVNGVARLHSEILKRTVFRNFYEYTP
                     DKFISITNGITHRRWLNQANPPLGALLDDLVGPGYRTSPERLNMLKHYKDDDSVLNRL
                     DEIKYTNKKRLAQYVKTNNHIDLNIDSIFDIQAKRLHEYKRQLLNALRIISIIIDINE
                     GNTSHVVPETFIFAAKAAPGYHRAKEIIQLIYALGEYINKNTFIKKYINVVFLENYSV
                     SLAEIIIPAGEISEQISLAGKEASGTGNMKFMLNGAITIGTYDGANVEIINEVGEDNF
                     FLFGMKDYEVEELWKNGYSSVIYYQKSLMLRKIIDFLNKGISGKSFESISRYLIGGPV
                     VDDPYMCFADFDSYMEVHQKASQLYFNNKKKWNQMSLMNIASAGVFSSDRTIREYADN
                     IWGIKPVI"
     gene            complement(13645..15102)
                     /locus_tag="PKH42_03115"
     CDS             complement(13645..15102)
                     /locus_tag="PKH42_03115"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOA19451.1"
                     /translation="MNKLKVLYVASEAAPFIATGGLGEVAGSLPKALVEKYKDKIDIR
                     IVLPLYQGIVDRNEFEFIGKTSVPLAWRQQYCGVYKTILNNVTYYFIDNEYYFKRSEC
                     YGHFDDAERFAFFSKAVLYILPMIKFMPDIIHANDWQTSLIPIYLSAKYSGYEGYRDI
                     KSIITIHNIEYQGVFDLAISEDVFDLHGKEKGIIEFKGAINLLKGALETAHFISTVSE
                     SYSKEILDDYYAHGLAEIIQKNSSKIKGILNGIDTEKYNPEDDTEIFENYNAESIQKK
                     YLNKINLQSLSGLPQDEDIPVIGIISRLVKHKGFDLIKESMEEILKEKVQFIILGKGD
                     RGYELFFKYLQESYRDKIYVRIGFDSDFAKKIYSGADLFLMPSISEPCGIAQMISSRY
                     GTVPIVRETGGLKDSIKDASLGKGNGFTFIDETPQALCEAVKRALIVYENKEDWRKLV
                     ETVMKMDFSWRKSAEKYFDLYSSLANFEENNNEGF"
     gene            complement(15113..16231)
                     /gene="glgD"
                     /locus_tag="PKH42_03120"
     CDS             complement(15113..16231)
                     /gene="glgD"
                     /locus_tag="PKH42_03120"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOA19452.1"
                     /translation="MNALGIIFSDMQDWSMTELASHRSVAAIPFGGRYRLIDFPLSNM
                     VNSGINKIYLITSSNYRSLMEHVGSGKDWDLSRKKGGILMIPPYSAGENHGLYKGRLD
                     ALKRVLDYIKFSKRNYVVLSDSDMIYDINYNKVIQYHEEKNSDITAVYIKTDINKSDS
                     IHKILYEFDEDGRADDILVYPVTSGNYNIGLNTWVMNKEFLESLIYDAIARNYKDFSF
                     DVLSARVHSLRIYGYEYTGFFAHIDSLQSYYENSMKLLNMDTLQNIFYSNTGPIYTKT
                     KDTAPAKYCKDAVVKNSLIANGCIIEGTVENSILFRDVRVGKNAVVKNSIIMQHGRIS
                     DNAKLDYIITDTYTFVNKDRVLLGYSNCPFFIKKNSVV"
     gene            complement(16233..17387)
                     /locus_tag="PKH42_03125"
     CDS             complement(16233..17387)
                     /locus_tag="PKH42_03125"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF003670.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOA19453.1"
                     /translation="MKLAKKECVVMLLAGGQGSRLGALTTKIAKPSVPFGGKYRIIDF
                     ALSNCVNSGLDTIGVLTQYQPLILNSYIGDGKSWDLDRMYGGIQILPPYQRVGDSEWY
                     LGTANSIFQNTNFIDQYSPEYVLILSGDHIYKMDYSLMIDFHKRMDADCTISVIDVPI
                     KEASRFGILSSDDSGRIIDFEEKPKRPKSTQASMGIYVFKWNKLKKYLEIDDKNPDSS
                     NDFGKDVIPLMLRSNEKLYAYKFKGYWKDVGTIDSLWQANMDLLGENGFELSEDWIIY
                     SRHAEKPPHYVGKNALINNSLVSEGAVILGNVENSVIFSGVVIEENALVKDSVIMADV
                     KINKNCIINYSIIDEMVTIGANLKIGKPKGENSEITVIPRESVINKMQEE"
     gene            complement(17374..19290)
                     /gene="glgB"
                     /locus_tag="PKH42_03130"
     CDS             complement(17374..19290)
                     /gene="glgB"
                     /locus_tag="PKH42_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOA19454.1"
                     /translation="MKKNDLPIYLFHQGTNYYSYNFLGSHIIENIGTVFRVWAPNAKN
                     VHITGNFCNWQKSAEYEMKKISDSGIWELFAPGIKEGELYKYIITAADNTIIYKADPY
                     AFSSQTKTETASIVYNINKYKWNDTNWFEYKKRINLYEIPINIYEVNLPSWKRKENGD
                     FYTYREMAPLLINHVKKHYYTHIELMPIMEYPFDGSWGYQVSGYYSPTRRLGEPDDLK
                     YFIDQCHQNGIGVILDWVPAHFPKDEHGLYKFDGDFLYEYKDSEKREIKGWGTHKFDF
                     GRTEVQSFLISNAVFWLDVYHADGLRVDAVSSMLYLDYDKKEGEWTPNIYGGNENLEA
                     ISFLKKLNESVFKNYPNTMMVAEESTAWPMVTKPTYLGGLGFNFKWNMGWMNDMIEYM
                     ETDPFFRKYKHKNITFSLHYAFSENYILPLSHDEVVHGKKSILNKMPGPYEEKFANVR
                     VLLGYMMAHPGKKLNFMGYEIGQFTEWDYNKEIEWFMIEYEYHGKLNTYIKNLNEFYL
                     KRSELWEVDYSWSGFKWISNDDYEQNIISFKRINKNGDELIAVVNFSPTERHNYIIGV
                     DEGIYTEVFNSNDEEFGGTGTGNKDRLISSQGSVHGYNSFVSLTLPPLSIIFLEKETK
                     YKVNKEGGKIYEIS"
     gene            complement(19470..20312)
                     /locus_tag="PKH42_03135"
     CDS             complement(19470..20312)
                     /locus_tag="PKH42_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19455.1"
                     /translation="MRRLISILLIAMILSTGCTSLDMADENIVKDVSDIITDTVFNGA
                     GNEKAERKESHTVKSENKNTLNLMSSVGDINIVPHQSDDTIININITAKAKEKKKAEE
                     IIDNYIYKINEEGSSIVIDTTFNELPNDINLVTDLTVYLPSTIQNLEVTANVGDVNIN
                     SFIGNMDIKNNVGEVILDKSNGSYNIKADVGGIILKDCIAVGNSEFKTSTGDIELSFE
                     SISKAVSIIAETGVGNIEVSLNDDSGYHAVINEFMKDERIQTKHDQGTSINLTTGVGK
                     IEFN"
     gene            complement(20325..21104)
                     /locus_tag="PKH42_03140"
     CDS             complement(20325..21104)
                     /locus_tag="PKH42_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19456.1"
                     /translation="MLNLIKYEFIRKYRLVSMILITMLALNLFLLTRGVGGSMLFLVL
                     SPIVLYVFYLADIIKMYSDDLNKKTGYMLFMTPNSGYKIISSKLLAAILEGFALVLIY
                     FIFILINGTYIIISAGNEIDYSQIIKAVDNLLSGSLGFNLGHVLVFLLAALAFLIAFI
                     TTVYTAMTIRKSIFSEIKFGGLFSFIIFLLINWVLSFLSSKFYDIITPYYDTVDTISS
                     TGRVSAGGLAMILLPMIAVLIIQAVSLTGISGYLLEKKINL"
     gene            complement(21097..21804)
                     /locus_tag="PKH42_03145"
     CDS             complement(21097..21804)
                     /locus_tag="PKH42_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19457.1"
                     /translation="MNKLSVLKITDLTKRYYNKTALENVSLDFEQGKIYGLLGPNGSG
                     KTTLMKVVAGLHKQTSGSVLINDEMLSYKSKAYVSYMPTENYLYENFKVIDILKYYNT
                     MYEDFRYDYAVNLLNDIDVNSDIKISNLSSGQIGKLKTVIALSRDAQIYMLDEPLNGV
                     DVLSRDTILDLIAKSHSENKIIIISTHLVSEIEKILDTVIFLKEGIVEVFGDAEELRQ
                     ERQLSVEGIYKEVFRNA"
     gene            complement(21797..22168)
                     /locus_tag="PKH42_03150"
     CDS             complement(21797..22168)
                     /locus_tag="PKH42_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA19458.1"
                     /translation="MEFNNNIPIYLQVIEKIKQDIVSGKLKPGEKMPSSRDYSNELGI
                     NFNTVARVYRELENEEIVFTKRGLGTFITESDQIIEKLRYTMAKNQITAFIDGMKQLG
                     YTKEDMIKFIETENNYLEGNE"
     gene            22372..23445
                     /locus_tag="PKH42_03155"
     CDS             22372..23445
                     /locus_tag="PKH42_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOA19459.1"
                     /translation="MHKKLFIPGPVEVKEDVLSKMATPLIGHRSEDASNLQRDISNNL
                     KKLFYTENEILLSTSSGTGLMEAAVRCCTKERAAMFSIGSFGDRWHEMAVSNGINADL
                     FKSELGSITRPEMVDDVLKTGKYDLVTITHNETSTGVMNPIDKISNVIKKYPDVIFCV
                     DAVSSAGGVKIETDKLGIDICITSSQKALGLPPGLSMCTISERAVEKARYIKNRGYYL
                     DLVQLYDTIKKKDYQYPSTPNLSLMFALNYQLKLIMQEGLDNRFNRHFAMAEYTRSWA
                     KKNFALFADEKYASNTLTTVKNIRNISVKDLNIELGKRGFAISNGYGSLKEKTFRIAH
                     MADLTLEDVKELILNIEDILSIS"
     gene            23469..24248
                     /locus_tag="PKH42_03160"
     CDS             23469..24248
                     /locus_tag="PKH42_03160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOA19460.1"
                     /translation="MYDLNKISSYLNTNTIGQSIIQYDNLNSTFAKAKNIFSTCPDGT
                     VVLSENQSECILRFGNEWLCSPDKNIYLSIILKSINNNYLVPLTDVVACSSILKSIEE
                     LYNLDCRIKWPNDIFINNNKISSVKSEIIGKGAGIILSLYINVNMEEKIENLKATSIK
                     IEINNDVERELLIGTILNNIEYHYVEMINTGKAYHSVDIYNENLLFNNKVVGVIKRGR
                     KTVRKAVAKNIDSGGWLQVINEKGEEEILDPGETIIQYEET"
     gene            24235..>25236
                     /gene="dusB"
                     /locus_tag="PKH42_03165"
     CDS             24235..>25236
                     /gene="dusB"
                     /locus_tag="PKH42_03165"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOA19461.1"
                     /translation="MKKLKIGSIELDTNVVLAPMAGITDITYRTICKEMGAGLVHTEM
                     ISAKGLYYQDRKTEELMKINKNNRPVSMQIFGSDADIMSYVVEKYINNRNDVDIIDVN
                     MGCPAPKIVKNKDGCFLMKEPELAGKIINKIKRTTKKPVSAKIRSGWSKDSINAVEFA
                     KILEFNGLDMVTVHGRTREQYYSGKADYRIINEVKKSVNIPVVGNGDIFSPHDAVKMI
                     EETNCDAVMIGRGVLGNPWLIMNTVKILNNDMNFYTPSPLDKINMIKRHASMLADELN
                     EKVAILEMRKFAAWYMKGMKNSSQIRNIINKITSAEELYKILDKYIEVYCLNDLNGLN
                     "
BASE COUNT         8049 a         4454 c         4063 g         8670 t
ORIGIN      
        1 ggcatatagt tgacggttta ggcggcaaga cagaaggtgt tccaagagaa gatggatttg
       61 atattacggt tgcatcggaa gtaatggcag tattttgtct ttctaatgat attattgagc
      121 ttaaagaaaa tttatctaaa ataatagttg catacaacag agcaggcgag cctgtgacag
      181 ctggtcaatt aaaagctcac ggagccatgg ctgcactttt gaaggatgca ttgaagccta
      241 atttggttca aacactggaa ggaacgcccg catttattca cggaggtcct tttgcaaaca
      301 tagcccacgg ctgtaattca gtaattgcta caagaatgtc ttgtaaaatt gctgactacg
      361 cggtaactga ggcaggtttt ggagcagact tgggagctga aaaatttgtg gatataaaat
      421 gcagaaaaac aggattaaaa ccaagtgcag ttgttatagt tgcatcggtt agagcattga
      481 aacaccatgg tggagcagca aaggcagatt taggaaaaga gaacctggaa gcattggata
      541 aaggacttcc taatttatta aagcatgttg agaatattac tcaggtgttt aaaatacctg
      601 ctgttgttgc aataaacaag ttccctacag atacggaagc agaattgaaa ttgataaaag
      661 aaaaatgtat ggagttgggt gtaaatgtaa agctgtcaga agtatggggc aaaggaagcg
      721 aaggcggagt tgatcttgcc aatgaagtta taagacttac agagcaggaa agctcattgg
      781 aatttgccta tgatttagat atttccatca aggataagat aagggcaatt gcaagaaaga
      841 tatatggtgc cgatgatgtg gaattttcat caaaagcatc aaaagaaatt gctaattttg
      901 aaaaacttgg atttggaaat cttcctatat gcatggctaa aaaccaatat tcctttactg
      961 atgattcaaa ggtatttggc aggcctacag gttttaatat aacaatcaga gatataacaa
     1021 tatcagccgg agcaggattt ttagttgcat tgacgggaga tataatgaag atgccggggc
     1081 ttcctaaggt acctgcagca gaaaatatag atatagatgt tgacggaagg ataaaaggac
     1141 tgttttaatg agaggggacg gaatgtcccc ttctttatta ataatcctcg ctagcttttt
     1201 ctttcgatag cgacactatc agaacaccga gcaatatcag cacgcatgca aggatatttc
     1261 ttaatgttat cggttcattt ataaacaata tacccatcac tatgcttgta ataggttcaa
     1321 gcattccaac tatactcgta actgtaggac ctacatattt tactcccagg gctacgaatg
     1381 tatttgcaaa aaatgaaaca aagactgcaa ctaagaccgt aaaaaaccat cccttaaatg
     1441 tcattgtaaa aacaaggttt ttgcttatta taccaaaaat aaacaggtac atagatgaaa
     1501 aggcggctgt ataaaatgtt attttaaatt gatatattgt attcaaaccg cttttgtcca
     1561 taaataataa atatcctgca aaaaatacac ctgacataaa agctatcagt attcccgtta
     1621 tattgatact tccttcgaaa aataaagtaa ttcccattgt ggatagtatc aaagaaacta
     1681 ccttgtctct gcttattttt tcaccaaaaa taataataca cgcagcagta accaatacag
     1741 gataaatata atgaatagtt gtgctcattc caacagatat ataattatag gagccgtaaa
     1801 ggctgagtgc ggtagcggaa gctaacaatc ctaatattgc taattgtttt gtctggctct
     1861 ttgtaacttt taaaggtaca ttattatatt ttaacataac aaaaaaaaac ggaatggaaa
     1921 gaagatttct atagaatgta agtgatatgg gattgcttcc ttccaaatac gttaattttc
     1981 ccaatatagg agtgaaacca taaataaatc cggctaagat accgaatgtt aagccttttg
     2041 ttttattcat aattccctcc tatatcattt ttattttccg cagcactttt tatattttaa
     2101 tcctgaaccg caggggcatg ggtcatttct tcctattttt tcacctttta caactatctt
     2161 ggagcgattg aattcctttt ttatctgttt tcttttatct tcatcaaaga tattttccca
     2221 ttcctccatt ccgtaaagcc attcggcttt tgcatcatgc atattccata acagcttttc
     2281 aaagtcaaat tctgctttta tttcagattc ttcagttaaa tcatctaaga ctaattcttt
     2341 tttgaaactt gtgtttgcac cgtctaaaaa ccctatgtat tccaatgcgg acatcttatt
     2401 ttcttctgcc aattcaacta tttttccttc aattacagca gttttgtttt caagaattct
     2461 tctgtaacat tccgtttctt tctccatata ccctttaaaa aattcgttat attctccctg
     2521 ggttttaatt gcttctaagt tttctgtcca ttgtttatat aaactcataa cttcctccaa
     2581 aaaatatact gttcttttat tttatcatga aaatggaaaa attcaataaa aaaaagcgct
     2641 ccttagagcg cacaatttac aaaccccttt gccattctta accgatgaga ttcttcaggc
     2701 cgtgccttca gaatgacaaa ttcaaaatat actgccatta atggtgcata ataggattat
     2761 cttatttttc tttcaacaaa tctcttattt ccgtcaacag ctcttcctgt gtaggtgccg
     2821 gaggagcctc aggttcagga gcagcttctt cttgtttttt tagcttttct tttgatttgt
     2881 ttattaattt aataaacaga aaaatagata atgctacaat caggaagtct acaacatttt
     2941 gaataaaatt tccatacata attgctgctt caggtttaat tacggtgtca ccttccataa
     3001 ttgcttcgct cattacatac ttcatatctt taaaatctat tcctgccatt aaatatccca
     3061 aaacaggcat tatcaaatca tttaccacgg atgttacaat tttgccgaaa gctccgccca
     3121 ttataacacc gattgccagg tctaaaacat ttccttgaaa tgcaaatttt ttaaaatcgt
     3181 cccacataac ttgtcctcca taaataagat taaccgatgg taaggggata cctttatgta
     3241 cggttataat atattatacc caattttatg taatatcaac ataaaattgg gtatgtactt
     3301 caaatttgtt agtttttata tgaaactttt ccttcggata tgcctatttc aaagctgtcg
     3361 tcataagtga tgtatcttac tttattttca attaccgtac tttcattttc tcccttgaat
     3421 accacaaata catcattgtt ttgtgaagct ttttcaagaa tttcatttaa aatttttatt
     3481 tctcgtttat catttatatt gtcgatatca ccattcatgg ataataccaa taccttatct
     3541 tcaaaatcca tgaaggattt gatattgttc cacattgttg tatcagccga actcattgtc
     3601 ccgtttgaaa ttataccttc aaaatatact atatttccga ttccttcata ataataattt
     3661 ccctgtgtta ttgttaattt ttccgtatag tttttaattg atgaagtttt cattttatct
     3721 tcaaatttag tttcttctct taagcccagc tgtttgtctt tcggctcata caatatcctt
     3781 aaattgtcaa catatattgt acctgtatct tttcttgctt cgttaatttc cgccaagtaa
     3841 atatttttaa gtgttatcgg ataagcaata ccctccggta tttttgcttc aacccatttc
     3901 catcctgtcc agttaacttc ttcttcgaaa tcaattttca ccatagtgcc gtaggcatca
     3961 gttatccttg ttctaagcca atgattttta ttgtcgccgt aaacccacat accgattgcc
     4021 aaaggtttat cttcaagttt gattccttca ccttcatctc caaactctac gaaggcaata
     4081 ctttggtctg tcatttttgt aaagtcgtaa tccaatcgaa gtgcattttt cccttctttt
     4141 acaaagtcct ttgtaatgga tatagaacct gccgattctt caggatacaa gcttaatttt
     4201 aagttatcaa gattttcaaa acgatttaat gtcttatatc tgtatcctac cttaacttga
     4261 atatgtttta tggcatctcc aaaggttaaa gttaccgctc cggtatttgc aatatcactt
     4321 gatataaata ctccatcttc aatttttcca accctgtttc tataggaagc agtaatgtac
     4381 ttccatggta tatatgcgga attgccgtct ttatcaattc caagaaccgt gcctagttca
     4441 tatttatcat taaatcctag ggttaatgaa tcagtatcca acttcaatgc caccggcttg
     4501 tctaatatat ctatctcaat atgtccttct gcattcccgg catgggcggt aatagttgct
     4561 tttcccggtt tttcaggcat gaatacatta tttacaacct tacctgcacc ggcgggagaa
     4621 acagtgtaat ctacattttt caaatcaaat tctacaggag tataatattc attgtaaaac
     4681 tttaaatcta aatttgtttc tgttttatta aataccgtat tttgtgatgc tgttatttca
     4741 attttatcaa ctttgttact atcaggataa ttattaaata ctccgacacc cgatgctatt
     4801 tttctttccc ttccgtctga cgggaagtta acaacagtaa catttgcatt tcttaaaaag
     4861 tcaacagcca tggttgtgga gcctcctcca tccatattaa cagcattgta agcgcctaat
     4921 tctatcatta tttttgcaag ttctgtctgt tttgcaccca cataatctcg gtgcctcccg
     4981 tcaacagtta ccaaaattat ttccgtattg tctttgttaa aaccgattgc tgttcttgga
     5041 tttactccta agacctgcga acttatcaca ttttccctgc cgtctttaac caagtagttc
     5101 agtgcaccaa aagaccaatc aatatcttcc gggctaaaat ccaactcaat atttaaggat
     5161 acgggatgac cttccttgaa tgaattaagc attaattcca ttgtagcagg atttgagcta
     5221 gcaactatat acccgttttc cggaataatc gtagaaggct gattttttct tactgatgat
     5281 acgatattat caactacaac aacttcaact atatcctgtg ttgtatatcc gggagatgtt
     5341 ttattccagt cggatgtgag aattgtcgga ccccattcaa tatttgaagt tttattcatt
     5401 acaacaacgt tgaattccgt agaatccgag ccataaaggg tgattttggg gttccatacg
     5461 gaaatatcaa tttcatcttt taattttgaa acggtgggat atccgtcatt aaaaggaaga
     5521 ggagaagtta ttaatttatt gtctctgatt attggaccgt atgtatatgt ggggtccccc
     5581 atgtagaaaa agtctccgtt aacagctgca accgctcccg atgacctaat catggagctt
     5641 aaagttgatc tgtttgaaat gccgtcttca ttgttaatag gtttaatttc tgtatgctca
     5701 tcggttaaat ctgcccggat aacattaata ttcatccacc ctgatgatgt atacttttca
     5761 attctttcat atgtaatact ctttgagagt ctaatttctt cagataagtc gtaaacagta
     5821 taaagactgc tgccgtatgc tgaagatgat aataatagta ttgctgcagc agagatggct
     5881 gctattcttt taaacatcat gttaatcatt cctttctttt ttaaattata acataagtgc
     5941 aagacgaata cgcttgcttc gcaccgatac aatgagaaca ttaaatcaaa aagacttagt
     6001 acaagtttca tcttgtattc attttatcag aattcaccca taattgccat taatttaagg
     6061 atattagagc atgttaaaat atagcaaatc agggttttat ttatctatta tagacgtaat
     6121 tataataata tagttccata taatcaataa taatagtaat acgataaaaa tttaaacgtt
     6181 gacaataaaa aacggatgtg ataatattaa aaaaaatcct tatcaagagt ggtggaggga
     6241 cgaggcccaa tgaaacccgg cagcctgatt attcaaggtg ccaattcccg cggattttcc
     6301 ggaagatgag gctataggaa actatataac ctcttcttgt gaagaggttt tttagtgata
     6361 aattgttgat agcctatacg gccgttcaaa ttttcgagac gaatttattg cattcattac
     6421 gtgcaaaata ttaaaggtat gcaaaattta aatacaaaaa aggaaaggat aaaataatga
     6481 gaaaatggtt atttacatct gaatctgtta cagaaggtca tcccgataaa atgtgcgatc
     6541 agattgcaga tggcatctta gatgcaatta tggaacaaga tccaaatgga agggttgcct
     6601 gcgaagcaat tactaagacc ggatttgtac ttgtagcagg agaaatcagc acgagctgtt
     6661 atattgatat tccgcattta gtaagagaaa ctataaagaa tatcggttat gacaatgcct
     6721 tatatggatt tgattataaa acatgtgctg tatttacatc aattgaagag caatctcagg
     6781 acatagcatt gggtgttaat gaagcagatg aatataaaaa gaataataat gatataaatg
     6841 atatgattgg agcaggggac cagggtatga tgtttggttt tgcctgcaat gaaacacctg
     6901 aattgatgcc catgccgata tccttggcac ataagctggc aaagaagctt gcagaggtaa
     6961 ggaaaaacaa tatagtggat tacttaagac cggacggaaa aactcaggtt actgttgaat
     7021 atcatgataa taaacctgtt agaattgaca caatagttat atctacccag cataatcctg
     7081 aagttaacat agatacaata aaaaaagacc ttattgaaat tgtaataaaa ccaaccgttc
     7141 ccaatgaatt gttggatgag aacacaaaat atatcgtaaa tcccaccgga agatttgtta
     7201 taggcggccc tcagggagac tcaggattaa cgggaagaaa gataatagta gacacctacg
     7261 gcggatattc aagacacgga ggaggagcat tttcaggaaa ggaccccaca aaggttgacc
     7321 gttcagccgc atatgcagca agatatatag ctaaaaatgt agtcgcagca ggattggcag
     7381 acaaatgtga ggtagaagtt gcttatgcaa tcggagtagc aaaaccggtt tcaataatgg
     7441 ttgaaacatt cggtacaaac aaggttgatg atgaaattat acaaaaagca ataaccgaga
     7501 attttgattt aaggcctgct gcaattattg aaaaatttaa tttaagaaga cctatttatc
     7561 agaagcttgc agcttatggt cactttggaa gagaagacct tgatttacca tgggaaaaga
     7621 cagataaaac agatgagtta aaaaaatatt tataaaagat acggggacac ttcttttgag
     7681 gaatgtcccc atattaggag tatatatgtt tgaaagaagc acgggtatcc tattacccat
     7741 tttttcactt ccatcaaaat acggtatagg gactcttgga aaagaagctt ataaatttat
     7801 tgattttttg cgctcagccg gacaaaaata ctggcagttt ttacctatag gccatacttc
     7861 atatggggat tcgccatatt cttgtttttc tgtatttgca ggaaatccgt attttataga
     7921 cttggatgta ttgtttgaag aaggattcat tgaagaaacc gacttaaaag gtattgactt
     7981 cggaagcgat gatgagcgaa tcgattataa gaaaatattt gaaactagat atggaatttt
     8041 aaaaaaggct tatttaaata aagggatgtc ctgtgattta gcatctttta cctgtaataa
     8101 tccatgggtt tcggattatg cattgtttat gtctttaaaa tataaaaata atcaacttcc
     8161 ctggtataaa tgggatgaaa tgtttattaa aagagatata aatacacttc aaaagtataa
     8221 aaatgatttt tatgatgaaa taaacttttg gattttttta cagtatatat tttttgagca
     8281 atattttaag ttgaaggatt atgcaaacag ctcgggaata tcaataatcg gtgatattcc
     8341 tatttatgtt gcagaggaca gtgttgaagc atggacagaa aagaagctgt taatgttaaa
     8401 tgaagataat ctgcctatat gcgttgcagg tgttcctccg gatgattttt cagacgatgg
     8461 gcagctttgg ggaaatcctg tttataattg ggagtactta aaagaaacag gatacatgtg
     8521 gtggatagac agaattgcat tttcatttaa attgttcgat gttgtaagga ttgaccattt
     8581 cagaggattt gatgaatttt gggcagttcc ctacggctct gaaaatgccg taaacggtag
     8641 ctggatgcct gcttatggaa aacaaatgtt ccaaacatta aagaaaagtt tgggtaattt
     8701 aaatattatt gcagaagatt tgggaattat taccgacagt gttattaaat taaaaaatga
     8761 ttcaggatat gcgggaatga aagttttgca atttgcattt gacgggaata atgataaccc
     8821 ttatttgcct tataactatg aggaaaatga tgttgcatac actggtactc atgataatga
     8881 tacattaaag ggatggttta acaaacttac gcttaaagaa aaacaatatg ttttacaaaa
     8941 attaaatatt aatatggtta atgaaaaaga tgataacaat ttagtatacg agttgataaa
     9001 agcaatttat aaaagcaagg catccctttg tatagtacct ttgcaggatt ttctttgctt
     9061 aggctcggag gcaagagtta acataccgtc aacattgggc aacaactgga gatggagact
     9121 aaaaaactca atgattacag atgaattagc agaaaaaata aaagctttaa aaactgaaag
     9181 agatgttcaa aatttcaatt aatgtgtgaa tttataatga gtttacctca attatcgacg
     9241 tattaatcta aatttagaat atattcgacc tgaaaaatat cagtaaaata gcatttctat
     9301 caaaaaaaac acacaaaact tgaaattttg agcattcaaa gtttacagag ctatatatta
     9361 ataaaaaaaa gaaccgtcca taaaatatta tgatagtaaa atcaagtaat tttccttttc
     9421 tatattgaaa atattatttt ttatattatt tgtgtattca atcatatctt ctttgaattt
     9481 taaagtaatt tctttttgtg ataaatttac tgctataata atcctttcat tttcattaaa
     9541 tctttcgaac acaaaaactt ccttatcatg aattaaacat ctgtatttac cttcagcaaa
     9601 aattttattt gttcttcgta attttgtaat aaataaataa aagtcaagta tctctttatt
     9661 ttctttcccc caaggatagc atcttctatt gaaagggtca tcccatcctt caattcctgc
     9721 ttcgtcgccg taataaatgc agggaatacc gggtagagtc atttgcagca agactgcaat
     9781 ttttaaaaac ttaattcctt gctccaattc ctcgtatgaa agtttgcttt ctgccatttc
     9841 gcttctgctt gaaggtactc ttttgcttcc caatattgtc agtattcttt cagtgtcatg
     9901 agttcccagc acattcatta ggcagtttac ggtatcagga ggatatgttt caataacttt
     9961 attcattata tgattaagat tagtgcagtc tttatttttt acaaattcga ttattccttt
    10021 tcgcagggga taatttgtta cagaatccaa ttgctttccg caaagatact ctctcagttt
    10081 gttgtatgaa tacttatttg aagcatcttc ccatacttca ccgattatca gtgcatcttc
    10141 attattcttt tttattgaat ttctaagagc aataatgaat tcatccggga gttcgtcagc
    10201 aacatccaaa cgccatccca taatgcctgt tttttgccag tgggtgataa caccgttttt
    10261 ccctgttatg aaatttatat aagagtcatt gttttcattc acttcaggca atgtttttat
    10321 accccaccag ctcacataat catcattcca attattgaat gtataccaat cgtaataagg
    10381 tgaatccttg ctttggtaag cacctatgga gtcgtacctg ccatatttat tgaaataaat
    10441 gctgtcatcg cctgtgtggc tgaaaactcc gtcaagaata acatgtatat tatatttctt
    10501 tgcttctttt ataagttttt tcaaggagtt ttcagtgccg aacatggggt ctatcttatg
    10561 ataatttccc gtatcgtact tgtggtttga atatgcttca aagattggcg ataaataaat
    10621 tatgctaaca cctaattttg ctatgtaggg cagtttttct ctgataccgt ctaaatttcc
    10681 tccaaagaag tcgttattta aaatctcacc ctttttatca ggaagatagt atggagtttc
    10741 accccaattc ctgattattg ctcctttttt tttgattatt ttagtcctga aaaacctatc
    10801 cacaaaaata tgatatataa ttcctccctt agcccaattt ggagttatat atttatcatc
    10861 atatatcagc agttcatagt ctttggattt ttcattatca aaaataaagg aataatagta
    10921 atgaccatat tcctctatgg aaagcatagc ttggtagata tcatagttct tttcttgtga
    10981 tatccaatgc atttttattt tatcgtgaaa agtcctactg taatcatgcc ttttttcaat
    11041 caataattcg ggtgcacaaa tgtttcgctt tacaagtatt ttaaatgtaa ttatttcatt
    11101 tgatttaatt ccgccttgag ggtatctgta aaattcagat tgcgaattaa atttatacat
    11161 aatgcctcct tgttgtaatt cctatgaata atctcatgag atccttcgct aacgctcagg
    11221 atgacatcat aaacacatta tattaccggt ttaatacccc atatattgtc ggcatattct
    11281 cgtattgttc tgtcggaaga aaaaacacct gctgatgcta tattcattaa tgacatttga
    11341 ttccacttct ttttattatt gaagtacagc tgggatgctt tttggtggac ttccatatag
    11401 ctgtcaaagt ctgcaaaaca catgtaaggg tcatccacga caggtcctcc tatcaagtat
    11461 ctggatatgg attcaaagga ttttcctgaa atccctttgt ttaaaaaatc aataattttt
    11521 ctgagcatga gacttttttg ataatatatt actgatgaat atccgttttt ccacaattct
    11581 tcaacttcat agtctttcat tccgaacaaa aagaaattat cttctccaac ttcattaatt
    11641 atttcaacat tagcaccgtc ataagttcct atggttattg ctccgttaag cataaatttc
    11701 atatttcccg ttccgcttgc ttcttttcct gccaaagata tctgttcgct tatttcaccg
    11761 gcaggaatga ttatttcagc caggctgact gaataattct ctaagaaaac cacattaata
    11821 tattttttta taaaggtgtt tttgtttata tattcgccta atgcataaat aagctgaatt
    11881 atttcctttg ccctgtggta gccgggagca gcttttgcag caaatataaa tgtttccggc
    11941 actacatgtg aagtattgcc ttcatttata tcgattatga tgcttataat tctaagagca
    12001 tttaacagct gccttttgta ttcatgcagt cttttcgcct gtatgtcaaa aatcgaatct
    12061 atgtttaaat cgatatgatt attagttttc acatattgtg caagcctttt tttatttgta
    12121 tatttgattt catcaagtct gtttagcaca ctgtcgtcat ccttataatg ttttaacata
    12181 ttaagtcttt ccgggcttgt tctatatccg ggtccgacaa gatcatcaag cagagctcca
    12241 agtggaggat ttgcctgatt aagccatcgc ctgtgagtta taccgtttgt tatgcttata
    12301 aacttatcag gtgtgtattc ataaaagttt ctgaaaacag ttctttttaa tatttccgaa
    12361 tgcagtcttg ctacgccgtt cactttgtga ctgcctatta tacacaaatt agccatattt
    12421 actttgccat agccgaatat ggacatatta aaagctttgt cccggttatt gtatttagct
    12481 gatgcttctt ctatgaatcg cctgtttatt tccttaatta tcatatatat tctcggaagc
    12541 ttagtttgaa cgatattatt atcgaatgtt tccagtgctt ccttcaatac cgtgtgattt
    12601 gtgtaggata ccgtattaac tacaatcgtc catgcttttt cccacgataa tccgtattca
    12661 tccataaaaa tacgcattag ctcaggaatt atcaatgaag ggtgggtatc atttatatga
    12721 attgcatttt tttcactgaa gttatccaat gaattgtatt ttttcatatg atttttaaca
    12781 atattttgtg ctgatgcaga aaccaaaaaa tattgctgct taagtcttaa tattttaccc
    12841 tcattatggt catcggcagg atataatact tttgatattg cttctgccat tgagtcttca
    12901 gataaggccc ttatatagtc tccctgcgaa aacaaattca tatcaatact gtttatactt
    12961 cgggattccc aaagccttaa aaggctgaca cctttgctgt tataaccgga aatcatcata
    13021 tcataaggta ttgcttctat ttccgtaggg caatcatgat gtatcttaag tccttcgccg
    13081 gtccattctt ctcttattgt gccgtcaaac tttacaatga ctctttcgtc tgtcctgggc
    13141 ataagccata catctgaagt agtcagccag tcatcgggaa gctccgtctg ccatccgtca
    13201 attaactttt gtttaaaaag tccgaattca tacttaattg aaaaccctac tgccgcatag
    13261 tcttgtgttg caagagagtc catgaagcag gcagcaagcc ttccaagtcc tccgttgcct
    13321 aagcccggat ctctttctat tgaatatagt tcttcgggag taatgttata cttcttcaga
    13381 gcttctttcg cctcatcttc aatacctaaa ttaaacaggt tattgcgtag agattttccg
    13441 ataagaaatt ccatgcatat atagtatact tgcttatgtt cattctgcat tcttttgaat
    13501 tcatttcttt tttttaagag caaatccctt attacaattg aaaatgccct gtaaatttga
    13561 tttatagtcg ccttctcagg ctcagtattc caatatcgaa taatttcttc atctattaaa
    13621 cggttaatca gtgttgcatc gattttagaa tccttcatta ttattctcct caaaatttgc
    13681 cagactgcta tacaagtcaa aatatttttc tgcagatttt ctccagctga aatccatttt
    13741 cataactgtt tcaactaatt ttctccaatc ctctttattt tcataaacta ttagagccct
    13801 tttgacagct tcgcacaatg cttgaggggt ttcatcaatg aatgtaaaac cattgccttt
    13861 tccaaggctt gcatccttta tcgaatcctt aagtcctccg gtctctctaa caatcggaac
    13921 agttccgtaa cgggaagaaa tcatttgagc aataccgcaa ggctcactta ttgaaggcat
    13981 cagaaataaa tctgcacctg aataaatctt ttttgcaaaa tcagaatcaa agccgattct
    14041 tacgtatatt ttatcacgat aactttcttg aaggtattta aagaataatt catatcccct
    14101 gtctcccttt ccgagtatga taaactgaac cttttccttt agtatttctt ccatggattc
    14161 ttttatcagg tcaaatccct tatgcttaac cagtcttgat ataatcccta ttacgggaat
    14221 gtcttcatcc tgcggcagcc ctgataatga ttgtaaattt attttgttta aatatttctt
    14281 ttgaatactt tctgcattgt aattttcaaa tatttctgta tcgtcttcag gattgtattt
    14341 ttcggtatct attccgttta atattccctt aatcttggat gaattttttt gtataatttc
    14401 tgccagcccg tgagcataat aatcatctaa tatttccttt gaatagcttt cgcttaccgt
    14461 actaataaaa tgagcggttt ccaaggcacc tttcaaaagg tttattgctc ctttaaattc
    14521 aattatacct ttttcctttc cgtgcaagtc aaatacatcc tctgaaatag ctaaatcaaa
    14581 caccccttga tattcaatgt tatgtattgt tattattgat tttatatctc tgtaaccctc
    14641 ataaccggaa tattttgcag ataaataaat gggaatcaat gatgtctgcc agtcatttgc
    14701 atgaataatg tcaggcataa attttatcat aggcagtata tacaataccg ccttagagaa
    14761 aaatgcaaat ctttcggcat catcaaaatg accgtagcat tcactccttt taaaatagta
    14821 ttcattgtct ataaaataat atgtaacatt atttaatatg gttttataaa cgccgcaata
    14881 ttgctgtctc caggcaagag gaacggatgt tttcccaata aattcaaatt catttctgtc
    14941 cacaattcct tggtacaaag gaagtactat ccttatatct attttgtcct tatatttctc
    15001 gaccaatgct ttcggcaatg acccggcaac ttcaccaagt ccgccggttg ctataaaggg
    15061 tgcagcttcg gatgcaacat ataatacttt taatttattc atataatcac tcctatacaa
    15121 ctgagttttt ctttataaag aagggacagt tagaatatcc caacaaaacc ctatccttgt
    15181 tcacaaaagt gtatgtgtcg gttattatgt agtccaattt ggcattgtcg cttattcttc
    15241 catgctgcat aataattgaa tttttaacaa ctgcattctt gcctactctc acatctctga
    15301 aaagtatgga attttcgaca gttccttcaa tgatacagcc gttggcaatc aaagaatttt
    15361 taacaactgc atctttacaa tacttagcag gagccgtatc ttttgttttt gtatatatgg
    15421 gacctgtatt tgaataaaat atattttgca atgtatccat atttaaaagt ttcatactgt
    15481 tttcataata gctttgcaaa gagtcaatat gtgcaaaaaa tccggtatat tcatatccgt
    15541 aaattcttaa agaatgaacc ctggcagata aaacatcaaa gctaaaatcc ttgtaatttc
    15601 ttgcaatagc atcataaata agggattcca agaattcctt gttcataacc catgtattta
    15661 atcctatatt gtaatttccg cttgtaaccg gataaactaa gatatcatca gctcttccat
    15721 cttcatcaaa ttcatataat attttatgaa ttgaatcaga tttatttatg tctgtcttaa
    15781 tatagacggc agtaatatcc gaattttttt cttcatggta ttgtattacc ttattataat
    15841 taatatcata aatcatatcg gagtctgata atacaacata atttcttttt gaaaatttga
    15901 tataatccaa aactcttttc aaggcatcga gtcttccctt ataaagcccg tggttttctc
    15961 ctgcagaata ggggggaatc ataagtatgc cgcccttctt tcttgacaag tcccaatctt
    16021 taccggaacc tacatgttcc attagagagc ggtagttgct gcttgttatc aggtatattt
    16081 tatttatgcc ggaattaacc atatttgaga gcgggaaatc tataagcctg tacctgcccc
    16141 caaaaggtat tgcggcaact gagcgatgag atgctaattc tgtcatgctc caatcttgca
    16201 tatctgagaa aataattcct aacgcattca tattattcct cctgcatttt atttataaca
    16261 ctttcacgcg gtattacggt aatttcagag ttctccccct ttggtttgcc aatttttaag
    16321 ttggctccga ttgttaccat ttcatctatt atcgaataat taattataca atttttattt
    16381 atttttacat ctgccattat cacggaatct tttacaagag cattctcttc tataactact
    16441 cctgaaaata ttactgaatt ctctacattt ccaagaatta ctgcaccttc cgatactaat
    16501 gaattattaa taagagcatt ctttcctaca tagtgggggg gcttttctgc atgccttgaa
    16561 tatattatcc aatcttctga taattcaaag ccattctcgc ccaaaagatc catatttgcc
    16621 tgccatagtg aatctatggt accgacatcc ttccaatatc ccttaaattt gtaagcatac
    16681 aacttttcat ttgatcttag cattagagga ataacatctt tcccgaagtc atttgatgag
    16741 tcgggatttt tatcatctat ttcaagatac tttttaagct tgttccattt aaatacatat
    16801 atacccatag aggcttgagt actttttggc ctttttggtt tctcttcaaa atcaattatt
    16861 cttccgctgt catccgagct taaaatcccg aagcgggaag cttcctttat tggaacatca
    16921 attaccgata tggtgcaatc ggcgtccatt ctcttatgga agtcaatcat taaggaatag
    16981 tccattttat aaatatggtc accggataaa ataagcacat attcaggaga gtactggtct
    17041 atgaaattcg tgttttgaaa aattgaatta gctgtaccca agtaccattc cgaatcccct
    17101 actctttgat aaggaggcag tatttgaatt cctccgtaca ttctgtctaa atcccaagac
    17161 tttccgtcac ctatatacga atttaatatt aatggttgat attgtgtcaa aacacctatt
    17221 gtatcgaggc cggagttaac acaattagat agggcaaagt caattattct gtatttgcct
    17281 ccaaacggta cagaaggttt tgcaattttt gttgttaagg ctcccaatct gctgccctga
    17341 cctccggcaa gcagcatgac aacacattct tttttagcta atttcataaa ttttccctcc
    17401 ctctttgtta actttatatt tagtttcttt ttctaaaaag attatcgaaa ggggcggcag
    17461 ggtcaatgat acaaaagaat tatatccgtg taccgaacct tgagatgata ttaatctatc
    17521 cttatttccg gtgcctgtcc ctccaaattc ctcatcattg gaattaaata cttcagtgta
    17581 aataccctca tcaacgccaa ttatatagtt atgtctttct gtaggtgaaa aattaaccac
    17641 cgcaatcaat tcatcaccat tcttatttat tcgcttaaat gatataatat tttgctcata
    17701 atcatcattt gaaatccatt taaagccgct ccatgaataa tcaacttccc atagctcaga
    17761 acgtttcaga taaaactcgt ttaaattttt aatatatgta tttaattttc catggtattc
    17821 atactcaatc ataaaccatt caatttcctt gttataatcc cattccgtaa attgtcctat
    17881 ttcatatccc atgaagttaa gttttttccc cggatgagcc atcatgtagc ccaaaagcac
    17941 cctgacattt gcaaactttt cctcataagg tccgggcatt ttattgagta tggatttctt
    18001 gccatgaaca acttcatcat gagacaaagg aagtatatag ttttcactga aggcataatg
    18061 aagtgaaaag gttatgtttt tatgcttgta ttttctgaaa aacgggtcag tttccatata
    18121 ttctatcata tcgttcatcc agcccatatt ccacttgaaa ttaaaaccca gtccgcctaa
    18181 ataagtgggt tttgtaacca ttggccaggc tgtggattct tctgctacca tcatggtatt
    18241 agggtaattt ttaaatacgg attcattaag cttttttaaa aaagaaattg cttcaagatt
    18301 ttcattccct ccatatatat tgggagtcca ctcaccttcc tttttatcat aatccaaata
    18361 taacattgag cttaccgcat caactctcaa gccgtcggca tgataaacat ccaaccagaa
    18421 tacggcgttt gatattaaaa atgattgaac ttcagttctc ccgaaatcaa atttatgagt
    18481 tccccagcct ttaatttctc ttttttcact atccttgtac tcgtaaagaa aatctccgtc
    18541 gaatttatac agtccgtgtt catccttggg gaaatgagca ggaacccaat caagaataac
    18601 acctatccca ttttgatggc attggtcaat gaagtatttt aaatcatccg gctcaccgag
    18661 acgcctggtt ggagaatagt aacctgaaac ctggtagccc caggaaccgt caaagggata
    18721 ttccataatg ggcataagct ctatatgggt atagtaatgt tttttaacat ggtttatcaa
    18781 tagcggcgcc atttctctgt atgtataaaa atccccattt tctttacgct tccaagaggg
    18841 tagattaact tcgtaaatat ttatgggaat ttcatataag ttaattcttt ttttatattc
    18901 aaaccaattt gtatcattcc atttgtattt gttaatatta taaacaattg atgccgtttc
    18961 cgtcttagtt tgtgaagaaa atgcatatgg atctgcctta tatataattg tattatccgc
    19021 agcagtaatt atatatttat acaattcacc ttcctttatg ccgggagcaa acaattccca
    19081 tatgccgcta tcagaaattt ttttcatttc atactcggca cttttttgcc aattgcaaaa
    19141 attgcctgta atgtgcacat tttttgcatt gggtgcccat actctgaaaa cggtgcctat
    19201 gttttctata atatgcgagc ctaaaaaatt atatgaataa tagtttgtac cttgatggaa
    19261 taaataaata ggtaaatcat tctttttcat aaaagccccg gtaaaattta tttgttctta
    19321 tttaaagtat acgacaaaat aaataacttt taaagatata atttataaac ataagtactt
    19381 ataatataag tataccttgc tttagacaag tttaaacaaa aaagacgaca aaaaaagaac
    19441 actcatatga atgccctttt atctaataat caattaaatt ctattttgcc tacacctgtg
    19501 gtaagattaa tgctggtacc ttggtcatgt tttgtttgga ttctttcatc tttcataaat
    19561 tcattgataa ccgcatgata tcccgagtcg tcatttagcg atacttcaat atttccaact
    19621 cctgtttctg ctataatgct cactgctttt gaaatgcttt caaaggacaa ttctatatct
    19681 ccggtacttg ttttaaattc agaatttcct actgctatgc agtctttgag aataattccc
    19741 ccaacgtcgg cttttatatt atatgaacca tttgatttat ctaaaataac ttcaccaaca
    19801 ttatttttta tatccatgtt cccgataaaa ctgtttatgt ttacatcacc gacatttgct
    19861 gtaacttcta aattttgtat ggtagaagga agatatacgg ttaaatcggt tacaaggttt
    19921 atatcattag ggagctcatt aaatgttgta tcaataacta tggagcttcc ttcttcatta
    19981 attttgtaaa tgtaattatc gataatttct tcagcttttt tcttttcttt tgcttttgct
    20041 gtaatattta tatttattat tgtatcatca gattggtgcg gtactatatt tatatctcca
    20101 acagagctca ttaagtttag agtattttta ttttctgatt taacagtgtg agattccttt
    20161 ctttctgctt tttcatttcc cgcaccgtta aatactgtgt ctgtaataat gtcggagaca
    20221 tccttaacta tattttcatc agccatatct aatgaagtgc agcctgtaga cagaatcata
    20281 gctattaata atatactaat taatcttctc atatttcccc ctgtttataa gttaattttc
    20341 ttttctaaaa gatatcctga aattcctgtt aaagaaacag cctgaattat taatacggca
    20401 atcattggaa gcaatatcat agctaatcct ccggctgaaa ctctgcctgt acttgatata
    20461 gtatcaacgg tatcataata gggagtaata atatcataga atttacttga taaaaatgaa
    20521 agtacccaat taatcaataa aaatattatg aaactaaaca gccccccaaa tttaatttcg
    20581 gaaaatatac tttttcttat tgtcatagct gtataaaccg ttgtaataaa tgctattaaa
    20641 aatgcaagtg cagccagcaa aaatactaat acgtggccca agttgaaacc aagactgccg
    20701 gacaataaat tgtcaacagc ttttattatt tgactgtaat caatttcatt acccgctgaa
    20761 ataataatat atgttccgtt tatcagtata aatataaaat atattaatac tagtgcaaag
    20821 ccttccagta tagctgctaa gagtttcgat gatatgattt tataaccgct gttaggtgtc
    20881 ataaacagca tgtaacctgt tttcttatta aggtcatcgc tgtacatttt tataatgtct
    20941 gccaagtaga aaacatataa aactatggga gataacacaa gaaataacat gcttccgcct
    21001 actcctcttg tcagtaagaa caaattcaat gctagcattg taatcagtat catagaaacc
    21061 aatctatact tcctaataaa ctcatactta attaaattaa gcatttctga atacctcctt
    21121 atatattcct tcaaccgaca attgcctttc ctgccttaat tcttcagcat cgccgaaaac
    21181 ctctacaata ccttctttta agaaaataac ggtatcaagt attttttcaa tttcactgac
    21241 taaatgtgtt gaaataataa ttattttatt ttcactgtga gattttgcaa ttaaatccaa
    21301 aatggtatcc cttgataaaa catctacacc gttcaaaggt tcgtcaagca tgtatatttg
    21361 tgcatctctc gataatgcaa taaccgtctt aagctttccg atttgacccg atgacaaatt
    21421 tgaaatttta atgtctgaat ttacgtctat atcatttaaa agattaacgg cataatcata
    21481 cctgaaatct tcatacattg tattgtagta ttttaatata tcaatgactt taaaattttc
    21541 atacaaatag ttttctgtag gcatgtatga aacatatgcc ttggacttgt aggataacat
    21601 ttcgtcattt attaatacac tgccgcttgt ttgcttatga agacctgcca caactttcat
    21661 aagagtggtt ttgccgcttc cgtttggccc aagtaagccg taaattttac cttgctcaaa
    21721 atccaagctg acattttcca gtgctgtttt attgtaatat ctttttgtta aatcagtaat
    21781 ttttaacaca ctcaatttat tcattccctt ctaaataatt attttctgtt tcgataaatt
    21841 taatcatatc ttccttggtg tatcctaatt gtttcattcc atcaataaat gcagtgattt
    21901 gatttttagc cattgtgtat cttaattttt caattatttg gtcagattct gttatgaatg
    21961 ttcccaaacc tctttttgta aatactattt cctcattttc caactcccta tatactcgtg
    22021 caaccgtatt gaaatttata cctaattcat tggagtagtc tcttgaagaa ggcatttttt
    22081 ctccgggctt tagtttccct gaaactatat cttgtttaat cttttctatt acttgtagat
    22141 atataggtat attgttattg aattccaact tatcaccttc ttttgtatat tagtgtcata
    22201 gtagaatagt atactaatac ttgagttatg tcaatagttt aatgttaaca tttcaaacaa
    22261 taattagtta caatttcgta acaattgctt ttaattataa cttgacataa aattgataaa
    22321 gttatataaa tggatagaga aattgagtta ttacatagag cggagggaac aatgcataaa
    22381 aaattattta ttcctggacc cgtagaagta aaggaagatg tgctcagtaa aatggctact
    22441 ccactgattg gtcataggag cgaggatgct tctaatctac aaagagatat ttcaaataat
    22501 cttaaaaaat tattttatac agaaaatgaa atattattgt caacatcttc aggtacagga
    22561 ttgatggagg cagcggtgcg atgctgcact aaagagaggg ctgctatgtt ttcaataggc
    22621 tccttcggcg acagatggca tgaaatggct gtttcaaacg gtatcaatgc cgatttattc
    22681 aaatcagaat taggaagtat tacaagacct gaaatggttg atgatgtatt aaaaacagga
    22741 aagtatgatt tagttacaat aactcacaat gaaacatcaa cgggagtaat gaatcctatt
    22801 gataaaataa gtaatgtaat aaaaaaatat ccggatgtaa ttttctgtgt tgatgcagta
    22861 agctctgcag gaggagtaaa aattgagaca gataaacttg gaatagatat ctgcataaca
    22921 tcatcacaaa aagcattggg gctgcccccg ggactctcta tgtgcaccat atccgagagg
    22981 gctgtggaaa aagcaagata tataaaaaac agggggtatt acttagattt ggttcaattg
    23041 tatgatacaa tcaaaaaaaa ggattatcag tatccgtcga caccaaattt atcattgatg
    23101 tttgctttga attatcaatt aaaactgata atgcaggagg ggcttgataa caggtttaac
    23161 agacatttcg ccatggctga atacacaaga agctgggcaa aaaagaattt tgcattattc
    23221 gcggatgaaa aatatgcttc caatacgctt accacagtga aaaacattcg aaatataagc
    23281 gtaaaggatt taaacattga attagggaaa agaggttttg ctatttctaa cggctatggc
    23341 agtttaaaag aaaaaacatt cagaattgca cacatggcgg acttaacttt agaagatgta
    23401 aaagaactta ttttaaatat agaagatatt ttatcaattt cttaaacata taatatcggg
    23461 ggatttaaat gtacgattta aacaaaatca gcagttattt aaatacaaat acaattggcc
    23521 agtctattat tcaatacgac aatttgaatt ctacgtttgc aaaagccaaa aatatatttt
    23581 caacatgtcc tgacggaact gttgtattat cggaaaacca atccgaatgt attttaaggt
    23641 ttggcaacga atggctttgc tcacctgata aaaatattta tttaagtatt attttaaaat
    23701 ctattaataa taattatctg gtacccttaa cagatgtggt tgcttgttct tctatattga
    23761 aatccataga ggagctgtac aacttggatt gcagaataaa atggcctaat gatattttta
    23821 taaataataa caaaatatcg tctgtaaaaa gtgaaattat aggcaagggt gcaggtataa
    23881 tattatcctt atatataaat gttaatatgg aagaaaaaat tgaaaattta aaagcgacat
    23941 caataaaaat cgaaattaat aatgatgttg aaagagaact tcttataggc acaatattaa
    24001 acaacattga atatcattat gttgaaatga taaacaccgg taaggcttat cattccgttg
    24061 acatatataa tgaaaacttg ctgtttaata ataaggtagt cggagttatc aaaaggggca
    24121 gaaaaacggt tagaaaagct gttgctaaga atattgacag cggtggatgg cttcaagtaa
    24181 tcaatgagaa aggcgaagaa gaaatattag atccgggaga aactataata caatatgaag
    24241 aaacttaaaa ttggaagtat tgaattagat acaaatgttg tgcttgcacc catggcaggc
    24301 ataacagata taacatatag aactatttgc aaagaaatgg gagcgggtct tgttcatacc
    24361 gaaatgataa gtgcaaaagg attgtactat caagacagaa aaacagaaga gcttatgaaa
    24421 ataaacaaaa acaacagacc tgtgtcaatg cagatttttg gaagcgacgc tgacataatg
    24481 tcgtatgttg tcgaaaaata tataaataac agaaatgatg ttgatattat agatgttaac
    24541 atgggctgtc ctgcacctaa aatagttaaa aacaaagacg gctgtttcct tatgaaagaa
    24601 cctgagttgg caggtaaaat aataaacaaa ataaaacgca caacaaaaaa acctgtttca
    24661 gctaaaataa ggtcgggctg gagtaaagat tccatcaatg ctgtagaatt tgcaaagatt
    24721 ttggaattta acggtttaga catggtgact gttcacggca gaaccagaga gcagtattat
    24781 tcaggtaagg ccgattatag aataataaat gaagtaaaaa aaagcgttaa tattcctgtc
    24841 gtaggtaacg gtgatatatt cagtcctcat gatgcagtta aaatgattga agaaacaaat
    24901 tgcgatgcag tcatgatagg aagaggtgtt ttgggaaatc cgtggctgat aatgaataca
    24961 gtaaaaattc taaacaatga tatgaatttt tatacaccat caccattgga taaaattaat
    25021 atgattaaga ggcatgcatc catgttggca gatgaattaa acgaaaaggt tgctattttg
    25081 gaaatgcgta aatttgccgc atggtatatg aagggcatga aaaattcttc acaaattaga
    25141 aacatcataa ataaaataac cagtgcagag gaattataca aaattttgga taagtacatt
    25201 gaagtttatt gcttaaatga ccttaacggt ttaaat
//
LOCUS       DAOPUE010000019        24431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2037_length_24431_cov_9.905194, whole genome shotgun sequence.
ACCESSION   DAOPUE010000019 DAOPUE010000000
VERSION     DAOPUE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 24431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24431
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_24431_cov_9.905194"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PKH42_03170"
     CDS             complement(<1..1325)
                     /locus_tag="PKH42_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOA19462.1"
                     /translation="MSRMSSMDEAYAKLTQDQIDEIEEKFAKADAAQKIFEKWSQEDI
                     DRTIRAIAWKVANTQTFKELVKMSIEESKLGDPVSRENKKYKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYAKPVGVIACVIPATNPDLTPAGNAIYALKARNAIIFSPHPRAAKTG
                     GRTIELMREGLRQVGAPEDLIQILGQGKAKINRAMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAYGVGAGNATMIWDETAKDELYKAAYNTMLSKTSDFGSGCSADGNIVMHESI
                     FEEGINALIEAGGYLLNESEQESIKKIMWDEECHRLPDSVAQSPQRMAQLAGFEIPED
                     RKFLIAMGTGSYTTAYEDVWCREKLTTLLAVHKYEGEFQNAIDIIKAIHEVGGKGHSV
                     GIYSYDEDHIHRLALEAQVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGT"
     assembly_gap    1379..1478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1479..2026)
                     /locus_tag="PKH42_03175"
     CDS             complement(<1479..2026)
                     /locus_tag="PKH42_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOA19463.1"
                     /translation="MILPSEIRLCEVGLRDGLQNEPKILTTEEKLKLAEGLINAGFKV
                     IELGSFMSPKAVPQMADTDELFRHFNNVEGVELRALVANKKGVERAINCGCGKIKLNV
                     SASKKHNLANINKTPEETVAGFAENVDLALENGLEVSGSISMPFGSPWEGVIPIKDVE
                     EIVEAYLRVGIKEISLSDAAGVA"
     gene            2238..3212
                     /locus_tag="PKH42_03180"
     CDS             2238..3212
                     /locus_tag="PKH42_03180"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOA19464.1"
                     /translation="MSVKNNILHILESNKGKTLSGQELADMLNVSRTAVWKAINSLKD
                     DGYNIKSTPNKGYSLSESSDMLSAEGIRLYLNEEFKNIPITVYKTIDSTNAKAKLLAV
                     QNAEHGTTIIAEEQTKGRGRFGRDFFSPAESGIYMSIILKAELSIENSVLVTTAAAVA
                     VCQAIEKFTSVYPKIKWVNDIFIGDKKICGILTEAVTNFESGMMDCVVVGIGINVKTR
                     NEEFPLELQNTAGSIFIDDDNYIRNQLAAEIINNLLKAANNLADKSFMKSYKQRSMVL
                     GENILYKKDNKWHEGYASYIDDYGGLVVYTEDGQKITLNSGEVSIKKK"
     gene            3293..3844
                     /locus_tag="PKH42_03185"
     CDS             3293..3844
                     /locus_tag="PKH42_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOA19465.1"
                     /translation="MYKEQTFSVRDITQIGIFTALTAVGAFISIPIGPVPITLQSFFV
                     LLSGIILGSKKAMFSQITYLLLGLIGFPIFSGFSGGLQHIFKPSFGFIIGYVAAAYVV
                     GKLTEEKTASQYLWAAVFAGTLIIYTFGLPYMYYILNIMLNSNLSIVKILQLGMFAFI
                     PGDMLKAVIIVFFAQKISPLLKG"
     gene            complement(3833..5164)
                     /locus_tag="PKH42_03190"
     CDS             complement(3833..5164)
                     /locus_tag="PKH42_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA19466.1"
                     /translation="MRVVIIGAVAAGTSAATEIRRNDKEAEIIIYEKDKYISYAGCGM
                     PYYISNEAEGFSSVVPRDKDFFKEKHNIDIYTEHEVVSVNPNNKTLRVKNKSGEIMDR
                     YDKLIIATGAMSVKPDLKGSDRDNVFLLRNINDMIDIKNFIDENKPKSAVIIGSGFVG
                     LEMCESFKLLGLDVTIIARSKISKGIDNDLSLYIEEHLKEKGVNVYTNTKTVEINDSG
                     VVLGDGSIVKADMVLLATGAKPNVTLAKEMGVELGATGAIKVDKYMKTNIEDVYSCGD
                     CIEMYYSFNGNPVYRPLGSTANKTGTIAGSNITGIEQEFKGILGTAIYRVFELTVGMT
                     GLSEEEAVKSGYNVVITIDKRPNKPEYMGGRPIIIKTVAEKGTRRLLGAQLIGYEGVD
                     RRLDVLVTAITFNAKAEDLMHLDLAYAPPYSTPRDNLYYTGAKLKAKLSAL"
     gene            complement(5194..5673)
                     /locus_tag="PKH42_03195"
     CDS             complement(5194..5673)
                     /locus_tag="PKH42_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA19467.1"
                     /translation="MKLLVPVDGSNASISAVKKALELAKKYNFSIKLISVVSAKSSEY
                     RRHENAWKGIDSSVISESEELERQLEMKIKENAQRLLNAIISKLDFSGIRVELEVLLG
                     EPYVKILETARNDNIDMIVMSNRGFSKIKRFFVGSVTQKVISEAPCPVLVVNTGFEP"
     gene            complement(5748..7136)
                     /gene="lpdA"
                     /locus_tag="PKH42_03200"
     CDS             complement(5748..7136)
                     /gene="lpdA"
                     /locus_tag="PKH42_03200"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOA19468.1"
                     /translation="MKIAIIGGGPGGYVAAIRAAQLNAEVTLIEKEHIGGTCLNKGCI
                     PTKVLLQTSGEFEAVNKSFKDLGIKISGAELDWKQLQKRKTKIIRKLVAGVDNLLKNN
                     KVTKIMGEGSFVNKNQLKVKTADGAEIFVDFDYAIIATGSKPVVIPIPGVELPGVVTS
                     DEVLSLEEIPNSMVIIGGGVIGSEFAEVFSAVGCKVTIIEMLPNIIANMDRDIVETLV
                     EKFKKKGVEIYTNTKVVSISKAEKGLSVNTLSSGNEISFNAEKVLLSIGRKPVTDNLA
                     LENVGVETNRGAVLVNKNMQTNVPNIYAIGDATAGVMLAHVASAEGIVAVESIMNKKS
                     NIDFKTIPYCVYTKPELAGVGLTEEQAREKGYDVKIGIFPMYINGKAMIEGEQEGLVK
                     YVVDAATGEVLGLHMSGPNATELIVEGALAVRLEATVDEIISTIHAHPTVAESLQEAA
                     HAVHNNAIHIPK"
     gene            complement(7148..7516)
                     /locus_tag="PKH42_03205"
     CDS             complement(7148..7516)
                     /locus_tag="PKH42_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HOA19469.1"
                     /translation="MVDRFIIEFGMGVDFHGQDVTKAAVKAAKDAISKSCLIGLNEIC
                     GFNQDNVDDKVLIDVTIGVTRPDEVKIDEVVKCFPVGKVTVKAVYGGLKTEGLYFSKF
                     GDKDNSIEVAVASVSVKIKK"
     gene            complement(7582..8232)
                     /locus_tag="PKH42_03210"
     CDS             complement(7582..8232)
                     /locus_tag="PKH42_03210"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOA19470.1"
                     /translation="MNKNCFVSWSGGKDSCLALYKAIEMGYKPVKLFTMFGTEKISSS
                     HRLDENIIKLQADSIGIEYLIGKSDFDEYEKVFVSNLKSFKDQGIDYGIFGDIDLDEH
                     RQWEERVCKSAGMEAILPLWKRDRKEIAKEFISLGFKAKIVVINTSMIDIKFLGKDLS
                     LSLIEEIEKAGADPCGENGEYHTLVYDGPIFKKPVDIKYDYEIIPVGETWAQIKVY"
     gene            complement(8379..9551)
                     /locus_tag="PKH42_03215"
     CDS             complement(8379..9551)
                     /locus_tag="PKH42_03215"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOA19471.1"
                     /translation="MNILILNCGSSSIKFQIAEMPDYNVIIQGSVERIGYEDAEFKFF
                     LVKQKIIQPVLSHEEGINLIINTLFKSNFGIETKDINAIGHRVVQGGDKQNISVLIDD
                     KVIDYIESIKDLAPLHNPNHLAGIYACRKLLPNVPQVAVFDNGFHMSLPRSTYIYGIP
                     YEYYEKYRIRRYGFHGIAFRSMAEEVDKILNETHKNYKIVNLMLGSGTTANALKYGKS
                     VEVSTGFTPHEGLIQSTRAGDIDAAAILYLMKKENLSIEQADELINKKSGWKGLSGIS
                     NDMREIYQASQSGNERAKLTIDAVCHRFKKYVGAYSAIMEGIDILVFSGGVGQNAPYI
                     REKVCKGLEYMGIELDEYKNQNTAGIISKDSSKVKVIVVNANEEKIIAQDTYEICK"
     gene            complement(9739..9882)
                     /locus_tag="PKH42_03220"
     CDS             complement(9739..9882)
                     /locus_tag="PKH42_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19472.1"
                     /translation="MADKNIKKENKKKKKKETKQANVSSVSYEQPVMSQPELVPKKKK
                     KDE"
     gene            complement(9992..10483)
                     /locus_tag="PKH42_03225"
     CDS             complement(9992..10483)
                     /locus_tag="PKH42_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOA19473.1"
                     /translation="MSIVKWNGRNLLKSIAENEKEATKLYRMFASEARIGEKFFELLA
                     KDEERHEKIYNALLEKYSDKLELEMEESDAEYMDLLVESNIGFDDELVEKAKKIFTKS
                     QIFDLAERAERDAVLFVTELQRLYPDLAKDEMAIILKEEKSHLKKVLERKKESQPMFG
                     RGM"
     gene            10625..11053
                     /locus_tag="PKH42_03230"
     CDS             10625..11053
                     /locus_tag="PKH42_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA19474.1"
                     /translation="MKLRDIMSREVACLSAEDTVEKAAQLMKTYNVGSIPICAQNSVI
                     GIITDRDIALRAVAVGDDGYLQVKKVMTANPVVGTPDMDVEDAVRIMSENQIRRLPVV
                     ENNRLVGIVSLGDISVEPRLQDEAEIALKNISKSATNRFS"
     gene            complement(11184..12089)
                     /locus_tag="PKH42_03235"
     CDS             complement(11184..12089)
                     /locus_tag="PKH42_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOA19475.1"
                     /translation="MELKNYQDLIDRIRKTDSKKRVAVAAAHDEHTLEAVFRAADDKL
                     VEPVLIGNKAKIIEILKELNVEYDLNSIISTDSDKESAEKTVELINSNMADFIMKGKL
                     QTADLLRAVVDKEKGLRTGRVMSHVAFLEIPAYHKLIAVTDGGMMMYPNAEEKKQILE
                     NVVDVFRAMGYECPKVAVLAAVETVNPKMPETVDADILKKMNQSGEIKDCLVEGPISV
                     DLTFNRESAEIKGFKSPVTGEADILLVPSITTGNIMCKALIEMAGATMAGMIVGAKVP
                     VVLASRGASSQEKYLSIVLSASSVK"
     gene            complement(12199..13497)
                     /locus_tag="PKH42_03240"
     CDS             complement(12199..13497)
                     /locus_tag="PKH42_03240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOA19476.1"
                     /translation="MAEFIVMPKMGLTMTKGMLTNWKKKEGDLVNKGDILFEVETDKI
                     TNKVEAKTSGILRKILVDKETVDVFVPVGIIGRADEDISELLEQASKELKTDKPQKEE
                     GPKETRELSPAVQAGDRVKISPRAKKIAKDLKVDYNLVQGTGPGGAITEEDIRKFAEG
                     TKAKVSPTAAIVAEQLGVDIFNIEKDTRIMKADVIKYKLNESLAKYANPQEYRQPMTN
                     MRKIIAKRMLHSVQTSPSVSFNIKVDTTGMKELRDELKDTFKVSYTDILVKIVSEVLL
                     EFPLLNSTLDGDEIITRNYVNMGVAVALPAGLLVPVVKFTNVKGLKEISNEIKDLSER
                     AKTNDLLPDELTGGTFTISNLGMYGIESFTPIINQPEVAILGVNAIHKEPVVVNDEVV
                     IKPMMNLSLTADHRVVDGAVAAEFLAKLKKYIENPCLLLL"
     gene            complement(13513..14517)
                     /locus_tag="PKH42_03245"
     CDS             complement(13513..14517)
                     /locus_tag="PKH42_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOA19477.1"
                     /translation="MASIGIIANPASGKDIRRLVSHATVIDNNEKINIVERIVLGAQA
                     LGVEKVFVMPDSYNMGYRVEDKLNSCNELRCEINVVNMMRFDGMEDTVKAADYMEKND
                     DIKCVIILGGDGTNRAAAKSLKNTPIITISTGTNNVYPTMLEGTVAGMAAAAVASGRF
                     DDTRYVTKDKRIEIYKDGDLIDIALIDAVISKDVFIGSKAIWNIDTIEKIIATRSHPA
                     SIGFSSLVGCKKIIYPEDDFGAYVDINSGSVRIKAPVAAGVVESVSVSEPVILRLEDE
                     YEFTAKDRGTIALDGEREIEFKKDQKLIFKITREGPYHVDVIKALETAQENNFFIIKN
                     "
     gene            complement(14577..15551)
                     /locus_tag="PKH42_03250"
     CDS             complement(14577..15551)
                     /locus_tag="PKH42_03250"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOA19478.1"
                     /translation="MKKMTYAEGIREAMRFKMREDPNVILFGEDVGKFGGCFGVSAGL
                     WEEFGDDRIRDTPISEGVIVGAAVGSAACGIKPIAEIMFIDFITVGMDQLVNQAAKMR
                     YMFGGEINLPMVIRVPAGAGIGAAAQHSQSLEGWLTHVPGLKVVYPSNARDAYGLLLS
                     SIEDQNPVVFIENKVLYAMQNDVDENELKPIPLGKARIAKEGTDVTIITYGRQVYDAL
                     EAAEILAKDGINAEVIDLRTLYPLDKETVFNSVEKTHKVVLVSEECKRGAYTGEIAAM
                     IAEERFYECDAPIMRVGALNTPIPFATDLESYVLPKARDIELAVKSLF"
     gene            complement(15548..16549)
                     /locus_tag="PKH42_03255"
     CDS             complement(15548..16549)
                     /locus_tag="PKH42_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOA19479.1"
                     /translation="MGGSILKITNEQLKEMYLTMLKIRKFEKSASELFAAGKIPGFVH
                     LYIGEEAVATGACANLREDDYITSTHRGHGHIIAKGGELKYMMAELFGREDGYCKGKG
                     GSMHIADATKGILGANGIVGAGHNIAVGSGLAIQHKKTDQVCVCFFGDASTNQSTFHE
                     ALNLASIWKLPVIFVCENNGYGISVSQKRHQAIADVSVRASAYNMPGITVDGNDLFAV
                     YEAVSEAVARARKGQGPTLIECKTYRWRGHFEGDPVVYRPEGELEAWIKKDPIPKVEK
                     YLLDNGVMNEEEMKKMNDDVDKMVEEAIEFAEKSPFPSLESALKDVYSDIVEEGRVR"
     gene            complement(16985..18820)
                     /locus_tag="PKH42_03260"
     CDS             complement(16985..18820)
                     /locus_tag="PKH42_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA19480.1"
                     /translation="MKKLLWRLIISAIFFVSAIIINDSQLKLIMYIISYILAGGDVVK
                     RAIENIKNGQVFDENFLMSVASIGAFFIGEAPEGVGVMLFYQIGEMLQSYAVGQSRRS
                     ISSLMDIRPDYANVLRNGEVLTIPPEEVEIGEIIVIKAGERVPLDGIVVEGNSMLDTS
                     ALTGESLPREVITGSELLSGCINMNGLLNAKVTKSYSESTVNKILELVENASSRKSKS
                     EKFITRFARVYTPVVVVIAVVLAIIPPLIIKGATFNEWIYRSLAFLVVSCPCALVISV
                     PLSFFGGIGGASRNGILVKGSNYLESLAKTEIIVFDKTGTLTKGVFKVQKIHSEKIKP
                     EELLKLAAYAESYSNHPISLSLKEAYKKDIDIEKISQVEEISGHGVSAAVENIKVLAG
                     NDKLMEKFKIPYNKETSEGTLVHIAADGEYAGYILIADKIKDDAFEAVSDLRKHNIKD
                     IVMLTGDSESVAINTARELGIDRVYAGLLPADKVEKVEELISQKSEKATLMFVGDGIN
                     DAPVLARADVGVAMGALGSDAAIEAADVVIMTDEPSKIAKAIEIAKNTIKIAYQNVIF
                     AISVKVIILVLSTLGLASLWAAVFADVGVTFIAVLNAMRALRLNN"
     gene            complement(18832..19053)
                     /locus_tag="PKH42_03265"
     CDS             complement(18832..19053)
                     /locus_tag="PKH42_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOA19481.1"
                     /translation="MTKTYKLQGLDCANCLAKMERKISKLKGVKSVTSNFFTEKMIIE
                     ADDGFMPEIEEAALKIIKKYEPQVVFEEA"
     gene            complement(19103..19474)
                     /locus_tag="PKH42_03270"
     CDS             complement(19103..19474)
                     /locus_tag="PKH42_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOA19482.1"
                     /translation="MSSNEGFVCDCEVIHGDIVNEVKKRMPDDEDIYDLSDFFKVLGD
                     STRMRIIWALDESEMCVCDIAVLLNMTKSAISHQLRALRNANLVKFRKEGKTVYYSLK
                     DKHIRDIYEIGMEHIKEKYNK"
     gene            complement(19563..19862)
                     /locus_tag="PKH42_03275"
     CDS             complement(19563..19862)
                     /locus_tag="PKH42_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19483.1"
                     /translation="MEKLFLQTENGLIPLDEKMIEKYNLVKGRKSPFTRYYVVDETGN
                     HNLGSTKETSSDDVDRMPAGEGAEDDEIVEFSDTGTILSQSEIIDFSHGTDSEEP"
     gene            20100..20411
                     /gene="trxA"
                     /locus_tag="PKH42_03280"
     CDS             20100..20411
                     /gene="trxA"
                     /locus_tag="PKH42_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOA19484.1"
                     /translation="MNTVKITSDNFEQEVLKSDKPVLLDFWAAWCGPCKMISPIIEEI
                     AKEVTDAKIGKVNIDEQPELASAFGIMSIPTLVKIKDGKVVNKMIGVRPKASIVQMLA
                     S"
     gene            20562..21227
                     /locus_tag="PKH42_03285"
     CDS             20562..21227
                     /locus_tag="PKH42_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOA19485.1"
                     /translation="MEKIMSSLVLKITALITMIIDHYGAIFQGNIIIYRIIGRLAFPI
                     YCFLLVEGYTHTSNIKKYASRLIAFAIISEIPFDLAFFGKIGFEHQNIFFTLFIGLAA
                     IYFIDNKEGKYSTNSAFIIIAASVIATLLRTDYQFIGIIYILAFYFTRNQAKHKKFLT
                     IGLIMFAVNLSAGMLQQFSLLALIPIYFYNKKEGRKNKILQISFYTAYPLHLIIFYLI
                     NTL"
     gene            21547..22233
                     /locus_tag="PKH42_03290"
     CDS             21547..22233
                     /locus_tag="PKH42_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOA19486.1"
                     /translation="MKKKILSLTLALIIALSSTSAFAAEQVYYTNFNIYDLCKNLGYY
                     YFEPVNTNNNIEIKTNIEKAPVNNGAETTQAPVNNGAETAQAPSNERATTEAATTAAP
                     SVSNYERRVVELVNVERQKNGLAPLTLDPAISNVARIKSKDMSDNNYFAHQSPTYGSA
                     GDMLSQHGIRYSAWGENIAAGQRTPEEVVSAWMNSPGHRANILGSHYTKIGVGYVNNG
                     RPYWTQMFTN"
     gene            complement(22398..23126)
                     /locus_tag="PKH42_03295"
     CDS             complement(22398..23126)
                     /locus_tag="PKH42_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOA19487.1"
                     /translation="MDYLYDGTFEGLMTCIYYHYKKEKATGIYEISEYQQSIVYEAEI
                     VKTDISKAKIVCDSINRQISKEAYIHVYYCYLSNDSKKENLILDFLIFAFKYGRDTMN
                     FYTHEKVLPINEIYKKVAREEHRVLGILRFSDIGGILFAKYSPDNDISILLADHFADR
                     YKHEKFIIYDEKRKKAVVYANNSWEIKENINIDNVEFSLDETKIHKLWKQYFTDLAIK
                     ERKNINLQFQFVPARYRKNMTEFK"
     gene            complement(23129..24376)
                     /locus_tag="PKH42_03300"
     CDS             complement(23129..24376)
                     /locus_tag="PKH42_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOA19488.1"
                     /translation="MEDNLFEKLRILSDAAKYDVSCVSSGVERKNNTVGGIGNASAAG
                     ICHAWSTDGRCISLLKILLTNDCIYDCKYCLNRVTNPIKRATFTPEEVAELTIQFYRR
                     NYIEGLFLSSAVEKSPDHTMENIYRVLELLRYKYNFWGYVHVKVIPGANPLLVQKTGM
                     LADRMSVNIELPTRESLSLLAPQKKMENILKPMSLIHNEISRAIEDKSHFKNAQKFVP
                     GGQSTQMIIGATPDSDKTILSASQNLYDKFRMKRVFFSAYVPIMSSPNLPAVSTMPPL
                     LREHRLYQADWLMRFYYYKSEELFTEVEQNLDLEFDPKMMYALRNIDKFPIEINNASY
                     EELLRIPGLGVTSAQRIIRERKNAQISYDSLKKMRTVIKRAKYFITVKGKYYGNTKID
                     PEAIKAEIRSKEIQRQMSIFELL"
BASE COUNT         7016 a         5118 c         3761 g         8436 t
ORIGIN      
        1 gtgccgcatc ctatgcttga tgtcatgggc attccgtttg tgaaggagcc ggcatttgat
       61 ttcgattgag gttgtctaac cattatcctg cctacttggg cttcaagagc aagtctatgg
      121 atatgatctt catcgtatga atatattccc acgctgtgac ccttaccccc tacttcgtga
      181 atggctttga ttatatcaat cgcattttga aattcacctt catatttatg aacagccaaa
      241 agtgttgtta atttttcacg gcaccacaca tcctcgtaag cagttgtata gcttccggtt
      301 cccattgcta tcaggaattt tctgtcttcg ggtatttcaa atcctgcaag ctgtgccatt
      361 ctctgaggcg attgggcgac cgaatcaggc aagcggtggc attcttcatc ccacattatt
      421 tttttaatgc tttcttgctc gctttcattc aataaatacc cgcctgcttc tattaatgca
      481 tttattcctt cttcaaaaat gctctcatgc ataacaatat tgccgtctgc agaacatccc
      541 gacccaaaat cggatgtttt ggaaagcatg gtattatatg ctgctttata taattcatcc
      601 ttggctgttt catcccatat cattgtagca tttcctgcac ctactccata agccggtgtg
      661 ccggagtgat atgcccttct tactacacct tgaccgccgg ttgcaattac caaatcacat
      721 tttgtattta aagcttccga catggctctg tttatcttag cttttccctg tcccaggatt
      781 tgtatcaaat cctcgggagc tcctacctgc cttaaaccct ctctcatcag ttcaatcgtt
      841 ctgcccccgg tctttgctgc tctgggatgg ggagaaaata ttattgcatt tctagcctta
      901 agggcatata ttgcattccc tgcaggtgtt aaatccgggt tagttgcagg tataacacat
      961 gctataaccc caaccggttt tgcatatttt acaattcctt tttcgggaat ttcctcaatt
     1021 attcctacgc ttttttgtct gagagcatca cgaagtactc cccttatttt atatttttta
     1081 ttctctctgc ttacggggtc tcccaattta ctttcttcga tgctcatttt gactaattct
     1141 ttaaaagttt gtgtattggc tactttccat gcaatagccc taatagttct atcaatatcc
     1201 tcttgactcc atttttcaaa aattttttgt gcggcatcag cttttgcaaa cttttcttct
     1261 atttcgtcaa tttggtcttg agtaagtttt gcataagcct cgtccattga actcattctg
     1321 ctcatatttc ctcctatatt atatagaaaa ctatatttta gaatagttta aacaatttnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cacacctgca gcatctgaaa
     1501 gggaaatttc ttttattcct actcttaaat aagcttcaac aatttcttct acatccttaa
     1561 taggaattac tccctcccaa ggagaaccga atggcatgga tatggagcct gaaacttcaa
     1621 ggccgttttc caaagcaagg tcaacatttt cggcaaaacc tgccacagtt tcctcaggtg
     1681 ttttatttat atttgcaagg ttatgttttt tgctggcaga aacattcagc ttaattttac
     1741 cgcaaccgca atttatcgcc ctttcaactc cctttttatt ggctacaagc gcacgcaatt
     1801 ccacgccttc aacattatta aagtgcctga acagctcgtc cgtgtctgcc atctggggaa
     1861 ctgctttagg gctcataaaa gaacctagtt ctattacctt aaaacctgca ttaattagac
     1921 cttcagcaag cttgagcttt tcttctgtgg ttaaaatttt aggttcgttt tgcaacccat
     1981 ctctcaaacc tacctcgcat aatcttattt ctgatggaag tatcaaaatg ccactccttt
     2041 caatattatt tatgcacgca atgttattaa tattatttta tattaataat atctatatgt
     2101 ccaatactag ttttatatag tatcaataga atttctctat ataacaaata taaattgcat
     2161 acaaggtcaa ccataattat tttttagttg acaatcttac aattgctttt tataatattg
     2221 tttattgagg tgaaattatg tcagtaaaaa ataatatatt acatatttta gagtcgaata
     2281 agggtaaaac tctttcaggt caagaattag cagatatgct gaatgtttcg agaacagcag
     2341 tttggaaagc aatcaattca ttaaaagatg atggatataa cattaaatca acacccaaca
     2401 aagggtactc tctgtctgaa tcgtccgata tgttatctgc cgaaggaata agattgtatt
     2461 taaatgaaga atttaaaaat attcctatca ccgtatataa aacaatagat tcaacaaacg
     2521 caaaggctaa gcttttagct gttcaaaatg cagagcatgg aacgacaatc attgcagaag
     2581 aacaaactaa gggaagaggc cggtttggaa gagatttttt ctcacctgcc gaatcaggta
     2641 tttatatgag tataattctt aaggctgaat taagcataga aaattcagtc ttagtgacaa
     2701 ctgcagcagc tgtggctgtc tgtcaagcca ttgaaaaatt tacaagtgtt tacccaaaaa
     2761 tcaagtgggt aaatgacata tttataggtg ataaaaagat atgcggcata cttacggaag
     2821 ctgttaccaa ctttgaaagc ggcatgatgg attgtgtggt agttggaata ggaattaacg
     2881 taaaaacaag aaatgaagaa tttcccttgg aattacaaaa tactgcaggc tcaatattta
     2941 ttgatgatga caattatata agaaatcagc ttgcagcaga aataattaat aatctgctga
     3001 aagcagcaaa taacttggca gacaagagtt ttatgaaatc atataaacag cgctccatgg
     3061 tattgggaga aaacatttta tataaaaagg acaataaatg gcatgaaggt tatgcatcgt
     3121 acatagacga ttatggagga ttggttgttt ataccgaaga tggacaaaaa ataacattaa
     3181 attcaggaga agtttcaatt aaaaagaagt gacaaaccct ggcgagtcat cttgagcgat
     3241 agcgaaggat ctcatgataa agacttaata aagaaagaat gagaggaaaa acatgtataa
     3301 ggaacaaaca ttcagcgtaa gagacataac acaaatagga atttttaccg cattaacggc
     3361 agtaggtgca tttataagca ttcccatagg tcccgtacca ataaccctgc aatcattttt
     3421 tgtactgctg tcaggaatta ttttaggctc taaaaaagct atgttttctc aaataacata
     3481 tttattgttg ggacttatag gcttcccgat tttctccgga ttttcaggag gattgcagca
     3541 tatatttaaa ccaagctttg gttttataat aggatatgtt gctgcagcct atgttgtagg
     3601 taaattaacg gaagaaaaaa ctgcctctca atatttatgg gcggcagttt ttgcgggaac
     3661 cttaattata tatacttttg gacttcccta tatgtattat attttaaata taatgcttaa
     3721 cagcaattta agtatagtta aaattcttca attaggtatg tttgcattta taccgggaga
     3781 tatgctgaaa gctgtaatca tagtattctt tgcccaaaaa atcagccccc ttttaaaggg
     3841 ctgataattt agcttttagt tttgctcccg tgtaatatag attatcccta ggtgttgaat
     3901 aaggaggagc gtatgctaaa tcaagatgca ttaagtcctc tgcctttgca ttaaaagtaa
     3961 tagcagttac caacacatca agccttctgt caactccttc gtaaccgatt aattgggcac
     4021 ctaataatct gcgtgttccc ttttcggcaa cagtcttgat aataattggt cttcctccca
     4081 tatactcagg tttattaggc cttttgtcaa tcgttataac aacattatat cctgatttta
     4141 cagcctcttc ctcagataaa cctgtcatcc ccacggtaag ttcgaaaact ctgtatatgg
     4201 cagtgccaag tattccttta aattcttgtt ctattcccgt aatattgctt ccggcaatcg
     4261 taccggtctt gtttgcagta gagcctaagg gtctgtaaac aggattgccg ttaaaagaat
     4321 aatacatttc gatacaatcg ccgcagctgt aaacatcctc aatatttgtt ttcatatatt
     4381 tatcgacctt gatggcaccg gttgctccaa gttcaactcc catttctttt gcaagggtta
     4441 catttggttt agcacctgta gcaagaagta ccatgtccgc tttcacaatg cttccgtcac
     4501 ctaatacaac cccgctgtca ttaatttcaa ctgtctttgt atttgtataa acattaactc
     4561 ccttttcttt taaatgttct tcaatatata aagataagtc attgtcgatt ccctttgaaa
     4621 ttttagacct ggcaataatc gtaacgtcca accccaatag tttgaagctt tcgcacattt
     4681 cgagacctac gaatccgctt ccgataataa cagctgattt tggtttattt tcatctatga
     4741 aattcttaat atcaatcatg tcatttatat ttcttaaaag aaatacattg tctctatcag
     4801 agccttttaa atcaggctta acagacattg ctccggtggc aattatcagc ttgtcatatc
     4861 tgtccataat ctcgcctgat ttattcttta ctcttaatgt tttgttattc ggatttacag
     4921 acactacttc atgctcagta taaatatcga tattatgttt ttctttaaaa aaatccttgt
     4981 ctcttggaac aacgctagaa aaaccttcag cttcattgct tatataataa ggcataccgc
     5041 aacctgcata tgaaatatat ttgtcctttt cataaatgat tatttctgct tccttatcat
     5101 ttcttctgat ttcggttgct gccgaagttc ctgccgcaac ggctccaatt ataacaacac
     5161 gcatattccc tccgctaatt ttataatttc ccattaaggc tcaaaaccgg tgttcacaac
     5221 taaaacaggg cacggtgctt ctgaaataac cttttgagta acagagccga caaaaaatct
     5281 ttttattttc gagaaacctc tgttactcat tactatcata tcgatattat catttcttgc
     5341 tgtttctaat atttttacat agggttctcc caaaagaact tctaattcta ctcttatacc
     5401 actgaaatcc aacttggata ttattgcatt taacaatctt tgtgcatttt ctttgatttt
     5461 catttccaat tgtctttcca gctcttcact ttccgaaatt acggagctgt caattccttt
     5521 ccatgcattt tcatgccttc tgtattcaga actctttgca gaaacaacag aaattagttt
     5581 tattgaaaag ttatattttt ttgccaactc caaagctttt tttactgcac ttattgaagc
     5641 gtttgaacca tctaccggaa caagcagttt cataaatatc actccttata cgttatatta
     5701 attttatatc cacaatttat gaggctaaac atattatatt ccaaaattta ttttggtatg
     5761 tgtattgcat tgttatgtac tgcatgagct gcttcttgga gagattctgc tacagtaggg
     5821 tgagcatgga tggttgatat aatttcatcc accgttgctt caagcctaac tgcaagagct
     5881 ccttcaacta taagctcggt tgcattcggt cctgacatgt gaagtcccaa aacttctccc
     5941 gttgctgcgt caactacata ttttaccaat ccttcttgct ctccttcaat cattgccttt
     6001 ccgtttatgt acattggaaa tataccgatt tttacatcat atcctttttc tcttgcctgc
     6061 tcttcagtta gtcctactcc tgcaagttca ggtttcgtat aaacgcagta aggaattgtt
     6121 ttaaaatcta tattagactt tttgttcata atagattcaa ccgcaactat tccttctgcc
     6181 gaagctacat gagcaagcat aactcctgct gtagcatctc cgatggcgta aatgtttggt
     6241 acatttgtct gcatattttt gtttacaaga actgcgcctc tgtttgtttc aacgccgaca
     6301 ttttccaatg caagattatc ggtaacaggt tttctgccta ttgaaagcaa caccttctca
     6361 gcattgaatg atatctcatt cccgctcgat agtgtattta cgcttagtcc tttttctgct
     6421 tttgaaattg aaaccacctt ggtatttgta taaatttcta cacctttctt tttaaacttt
     6481 tctactaagg tttcaacaat atccctatcc atatttgcta taatattagg cagcatttca
     6541 attatcgtaa ctttacatcc cactgcactg aatacttctg caaattcaga acctattact
     6601 cctcctccta taataaccat ggagtttggg atttcttcaa gagacaatac ttcatcgctt
     6661 gtaacaactc ccggcagttc aacccccggt ataggaatta cgacaggttt tgaacctgtc
     6721 gcaattattg catagtcaaa atcaacaaat atttcagcac cgtctgcagt ctttactttt
     6781 aattgatttt tattgacaaa gcttccttct cccattattt ttgttacttt attgtttttt
     6841 aacaggttat ccactccggc tactaatttt cttattattt tagtttttct tttttgtaac
     6901 tgtttccaat ctaattctgc tccggatatt tttatgccca agtccttaaa acttttattg
     6961 actgcttcaa actctcctga agtttgaagc agaaccttag taggtataca tcctttattg
     7021 aggcatgtac ccccgatatg ctctttttca attagcgtta cttccgcatt aagctgagcc
     7081 gccctaatag ctgctacata gccgccgggg cctccgccta ttattgctat tttcattatt
     7141 cctcccctta ctttttaatt ttaacagata cacttgcaac tgcaacttca attgaattat
     7201 ccttatctcc gaattttgaa aaataaagac cttcggtttt taaaccgcca taaaccgctt
     7261 ttacggttac ctttcctaca ggaaaacact ttacaacctc gtcaatcttc acctcgtcag
     7321 gacgagtaac tccaatcgta acatcaatca gcaccttgtc atcaacatta tcctggttaa
     7381 atccgcatat ttcattaaga cctataaggc aacttttgga tatggcatct tttgctgctt
     7441 ttactgcagc tttcgttaca tcttgcccat ggaaatccac tcccatacca aattcaataa
     7501 taaatctgtc cacgatatca cccctttata ttattagtat agtttataaa ataatgttta
     7561 ttcaacaata attgtatccc tttaatatac tttaatctgg gcccatgttt ctcctacggg
     7621 aataatttca taatcatatt ttatatccac cggtttttta aaaatgggac catcatacac
     7681 aagggtatga tattctccat tttcgccgca tgggtcagct cctgcctttt caatttcttc
     7741 tatcaaagac agacttaaat cttttcccaa aaattttata tctatcatac tggtgttgat
     7801 aacaactatt ttagccttaa atccaagcga aataaattct tttgctattt cttttctatc
     7861 cctcttccaa aggggcagga tagcttccat acctgctgat ttgcatactc tttcttccca
     7921 ctgtctatgt tcatctaaat ctatgtctcc gaatattcca tagtcaattc cctggtcttt
     7981 aaaacttttt agatttgaaa caaatacctt ctcatattca tcaaagtctg atttccctat
     8041 caggtattca attcctattg aatccgcctg taattttata atattctcat ccaatctgtg
     8101 agaggaactg attttttccg tgccaaacat ggtaaaaagc ttgacgggct tatatcccat
     8161 ctctattgcc ttatataatg caagacagga atccttgccc ccgctccatg aaacaaaaca
     8221 atttttattc aaattctacc tctctatact tagttattag ttgacagctt ataacagcat
     8281 tagtatatat ttttattaat tctacccaat aatgcataaa atactatagc aatgagtatt
     8341 ccgattgttt atggataatc ctttcctatc gctcagactt acttgcatat ttcatatgta
     8401 tcttgggcaa ttattttttc ttcatttgca ttaactacaa taacttttac ttttgatgaa
     8461 tctttgctga ttatacctgc agtgttttga ttcttatact cgtccagctc aattcccatg
     8521 tattccaatc ccttgcatac tttttctctt atataagggg cattctgacc tactccaccg
     8581 gaaaacacaa gaatatcaat accttccatt atggcactgt aagcacccac atacttttta
     8641 aacctgtggc atacagcatc aattgtgagt ttcgctcttt cgtttccgct ttgtgatgct
     8701 tggtagattt cacgcatatc gttactgata ccggaaagac ctttccatcc gctttttttg
     8761 tttattaatt catccgcttg ttcaatagat aaattttctt ttttcattaa atataaaatg
     8821 gcagcagcat caatatctcc ggctcttgta gactgtatca gtccttcgtg aggagtgaaa
     8881 cctgtgctca cttcaactga tttgccgtat tttaaagcat tagccgttgt tccgcttcca
     8941 agcattaaat ttactatttt ataattctta tgagtctcat ttaatatttt atcaacttct
     9001 tccgccatgc ttctgaatgc gataccatga aagccgtatc ttctgatacg atatttttca
     9061 taatattcat aaggaatacc atagatatat gtagaccttg gaagcgacat atgaaatccg
     9121 ttatcaaata cagccacctg cggtacattg ggaagaagct tcctgcatgc ataaattcct
     9181 gccaaatggt tagggttatg aagaggggcc aagtccttta tactttctat ataatctata
     9241 actttatcat caataaggac cgaaatattc tgcttatctc ctccctgcac aactctatga
     9301 ccgattgcat taatgtcttt tgtttcaatt ccaaaattac ttttgaaaag agtattaatg
     9361 attaaattaa tgccttcttc gtgacttagc acaggctgaa ttattttttg ctttactaag
     9421 aaaaatttaa attctgcatc ttcatatcct attctttcta cgctgccctg tataattaca
     9481 ttgtaatcag gcatttctgc tatctgaaat ttgattgatg agctgccgca gtttaatatc
     9541 aaaatattca tattccctcc tgatttaccg agaccatgat atatcccttt atcctttgct
     9601 gttaaaacaa tatgttgaga ttattcgaac tctctcttgt gacaaaaaac ttatttaatt
     9661 atacaacata taaaaaaaac aatcaattac tgcaataaag aaaatgtgtc cggtatatcc
     9721 gagacacatt tttagatact attcatcttt tttcttcttt ttaggaacta attcaggctg
     9781 actcataaca ggttgttcat aagatactga tgatacattt gcttgttttg tttctttttt
     9841 cttttttttc ttgttttctt tcttaatgtt tttatctgcc attttattaa ctcctttatt
     9901 tgcatttttc atttgtgtca aattgatttt gtgacactat tataatacaa atggaataat
     9961 aatgcattat ttttttaaag aagataatat tttacatgcc tcttccaaac attggctggc
    10021 tttctttttt tctttccaga actttcttca agtgagattt ttcttccttt aagataattg
    10081 ccatttcatc ctttgccaaa tcgggataca atctttgcaa ttcggtaacg aataatactg
    10141 catctctctc agctctctct gccaaatcaa atatttgtga tttagtaaat attttttttg
    10201 ccttttcaac tagttcatca tcgaaaccga tattgctttc aactaataaa tccatgtatt
    10261 ccgcatcgga ttcttccatt tccagttcca gtttatctga atatttttca agcagtgcat
    10321 tgtagatttt ttcgtgtctt tcttcatcct ttgccaacag ttcaaaaaac ttctctccta
    10381 ttcttgcttc acttgcaaac attctgtata acttggttgc ttctttttca ttttcagcta
    10441 ttgatttaag taaatttctt ccgttccatt ttacaatact catataaaat accctccttt
    10501 taatatataa atacttttac ccatcaggga ctgctttaaa caataaatac cgaaaattca
    10561 ttttttattc ataaatctga atacaacaaa gaataaaggt aaatatatat ttggaggtga
    10621 tattttgaaa ttaagagaca ttatgtcaag agaagttgca tgtttaagtg cagaagatac
    10681 tgttgaaaaa gctgcgcagc ttatgaaaac ttacaatgtc ggctcaattc cgatatgtgc
    10741 gcaaaacagc gtaatcggca taattaccga tagggacatt gctttaagag cagtagctgt
    10801 gggcgatgat ggttatttgc aggtaaaaaa agtaatgact gcaaatcctg tggtaggaac
    10861 tcccgatatg gacgttgagg atgctgtgag aataatgagc gagaaccaga ttagaagact
    10921 tcctgtcgtt gaaaataaca gattagtagg aatcgtatca ttaggtgata tttctgttga
    10981 accaagactg caggatgagg ctgaaatagc acttaaaaat atatcaaaat ctgctacaaa
    11041 cagattcagt taagatggac taaacaaatt tcaatgcgag atgcccaaga cccattcatt
    11101 agaagtatgg ggacacacct ttaacgcagg aaaatctttg agagtgcgtt aaaggaatgt
    11161 ccccgtattt tattccaaaa tatttatttt actgatgaag cagacaaaac tatcgataaa
    11221 tatttttcct gtgatgatgc gcctcttgat gccaatacta ccgggacttt tgctcccact
    11281 atcattcctg ccatagttgc gcctgccatt tctatcaatg ctttacacat tatatttcct
    11341 gtcgttatgc tcggaaccaa caatatatcc gcttctccgg taacaggact tttaaaacct
    11401 ttgatttctg ctgattccct gttgaaagtc aaatccacgg aaatgggtcc ttcaactaaa
    11461 caatctttta tttctcccga ttgattcatt tttttaagaa tatccgcatc aactgtttca
    11521 ggcatttttg gattcacggt ttcaaccgct gcaaggactg caaccttggg acactcgtat
    11581 cccatggcac ggaaaacatc gacaacattt tcgagtatct gcttcttttc ctctgcattt
    11641 ggatacatca tcatcccgcc gtctgtaacg gcaatcagct tatggtatgc cggaatttcc
    11701 aaaaatgcaa catgggacat taccctgcct gttctcaagc ctttttcttt gtcaactacc
    11761 gccctcaata aatctgcagt ctgaagcttt cctttcataa tgaagtctgc catgtttgaa
    11821 tttattaatt caacggtttt ttctgcagat tccttgtcgc tatctgtact aataatgctg
    11881 tttaaatcgt attctacatt aagctctttt aatatttcta tgatttttgc tttatttcct
    11941 attaatacag gttcaaccaa tttgtcatca gctgctcgga aaacagcttc caatgtatgc
    12001 tcatcatggg ctgcagcaac ggctacacgt tttttcgaat cagttttgcg aattctgtca
    12061 atcaaatcct ggtaattttt taattccatt gtttcctcct attgttgttt gtccgcttat
    12121 gtacaaaaca ttatttgtta tattatatca tcatttgcaa atacttcaac cgggatttga
    12181 gacatctcaa ggcagtatct ataataaaag caagcacgga ttttcaatgt atttttttaa
    12241 ttttgctaag aattcggctg caaccgcccc gtcaaccacc ctgtggtctg ccgtaaggct
    12301 taaattcatc attggcttaa taacaacttc atcatttact acgaccggct ccttatgaat
    12361 tgcgtttact cccaatattg caacttcagg ctgatttatg atgggggtga aggattcgat
    12421 tccatacatt cccagattac tgatggtgaa tgttccgccg gtcagttcat ccggcaataa
    12481 atcattggtt tttgctctct cgcttaggtc tttaatctca ttggaaattt cttttaagcc
    12541 ttttacgttt gtgaatttta ccacaggaac caggagtcct gccggaaggg caacggcaac
    12601 acccatgttt acataattcc gggttattat ttcatctccg tcaagggtgc tgtttaaaag
    12661 aggaaactca agcaatactt cagatactat ttttacaaga atatccgtgt aagatacctt
    12721 gaatgtatcc tttaattcat ctctcagctc tttcatcccg gttgtgtcaa cttttatgtt
    12781 aaatgatacg gatggagatg tttgaacgct gtgaagcatt ctctttgcaa taatctttcg
    12841 catattggtc atgggttggc ggtattcttg gggatttgca tattttgcca agctttcatt
    12901 taatttatat ttaattacat ctgccttcat tatcctggtg tctttttcaa tattgaagat
    12961 gtctactccc aattgttcgg caactatagc cgctgtcgga gatacctttg cttttgtacc
    13021 ttcggcaaat tttcttatgt cttcttccgt aatggcgccg cccggtcccg ttccctgcac
    13081 caaattataa tccaccttta aatcctttgc tatcttcttt gctctcggag aaatctttac
    13141 tctatctcct gcttgtacag ccggcgatag ttcccttgtt tctttgggac cttcttcctt
    13201 ttgcggctta tccgttttaa gctcttttga tgcctgctcc aacaattcag aaatatcttc
    13261 atccgcccta cctataattc caacaggcac aaagacatct acggtttcct tgtcaaccaa
    13321 gatctttctt aaaattccgg atgtcttggc ttcaaccttg tttgttattt tatccgtttc
    13381 cacttcaaac aggatatcgc ctttatttac taagtcccct tctttctttt tccaatttgt
    13441 cagcatgccc tttgtcatgg taagccccat ttttggcatt acaatgaatt cagccatatt
    13501 acctccttag tatcagtttt ttataatgaa aaaattgttt tcttgtgcag tttctaaagc
    13561 ctttataaca tcaacgtgat aaggaccctc tcttgtaatt ttaaaaatca acttctgatc
    13621 ctttttgaat tcaatttccc tttctccgtc taaagctatg gtgcccctgt ccttggccgt
    13681 gaattcatac tcatcctcta atctcaaaat taccggctcg cttacagata cggattccac
    13741 cactcccgct gcaacggggg ccttaatcct tacgcttcct gagttgatat ccacataggc
    13801 tccgaaatca tcttccggat aaataatttt tttgcacccc accagggagg aaaaaccgat
    13861 ggaagccggg tgagatctgg ttgcaatgat tttttcgatt gtatctatat tccatattgc
    13921 ctttgagcct atgaaaacat cctttgatat cacggcatct ataagggcga tgtcaataag
    13981 gtctccatct ttgtatattt caattctctt gtccttggtt acatatcttg tgtcatcaaa
    14041 tcgaccggaa gcaactgccg cagctgccat gccggcaacg gttccttcaa gcatggttgg
    14101 atatacgttg ttggttccgg tggaaatagt tattatggga gtgtttttta aactttttgc
    14161 tgctgccctg ttggtgccgt ctccccctaa tatgattacg cacttgatat catcgttctt
    14221 ttccatataa tcggcagcct tgacggtgtc ctccataccg tcaaaccgca tcatattaac
    14281 cacatttatt tcacatctaa gctcattaca gctgtttaac ttatcttcaa ccctgtatcc
    14341 catattgtat gagtcgggca ttacaaaaac cttttcaact cccaatgcct gggctccaag
    14401 tacaattctt tccactatgt tgattttttc attgttgtcg ataaccgtgg catgagatac
    14461 cagccttctg atatccttac cggaagcagg attcgcgatt attcctattg aagccatata
    14521 aacctcctaa cagcaggggc tgccgcatag tgcaacagcc ccggccttat cctagtttaa
    14581 aacaatgatt ttacagccaa ttcaatatcc cttgcttttg gtagcacata agactcaagg
    14641 tcggtggcaa aaggtatggg tgtatttaag gcacccaccc tcatgatggg agcatcacat
    14701 tcatagaacc gctcttctgc tatcattgcc gcaatttctc ccgtataggc ccccctcttg
    14761 cactcctcgc ttaccaaaac aaccttatgg gttttttcca ccgaattgaa tacagtttct
    14821 ttgtcaagag gataaagggt tcttaaatca ataacttcag catttatccc atccttagcc
    14881 aagatttcgg ctgcctccaa ggcgtcataa acctgtcgac cgtatgtgat gattgtaaca
    14941 tcagtccctt ctttagcaat tcttgccttc cctaagggta tgggctttaa ttcattttca
    15001 tcaacatcgt tttgcattgc ataaagtact ttattttcaa taaataccac aggattttgg
    15061 tcctcaatgg atgatagcag caagccgtat gcgtcccggg catttgacgg ataaacaact
    15121 ttaaggcccg gcacatgtgt cagccatccc tccagggact gggagtgctg ggctgcagca
    15181 ccaatgcctg ctcctgccgg tactcttata accatgggga ggtttatttc tccgccgaac
    15241 atgtacctca ttttggctgc ctggttaacc aattggtcca taccaaccgt gataaaatct
    15301 atgaacatta tttcagcgat gggttttatt ccgcatgcgg ctgagcctac ggcagcacct
    15361 actattacac cttccgatat gggggtgtcc cgaattctgt catccccgaa ctcttcccac
    15421 aagccggcac tgactccgaa gcatcctccg aatttaccca catcttctcc aaacagtata
    15481 acattgggat cttcccgcat tttaaaacgc attgcttccc ttattccttc ggcatatgtc
    15541 atctttttca tcttactctt ccctcctcaa ctatatcaga atacacatcc tttaaagctg
    15601 attccaatga tgggaagggg ctcttttcgg caaactctat tgcctcttca accatcttgt
    15661 ccacatcgtc attcattttc ttcatttcct cttcattcat aacaccgtta tccagcaagt
    15721 atttttctac cttgggaatt ggatctttct taatccatgc ttccaattct ccttccggcc
    15781 tgtacacaac cgggtctcct tcaaaatgac ctctccacct gtatgttttg cactcaatga
    15841 gggttggacc ttgtcctttt ctggctcttg caactgcttc gctcacagcc tcatataccg
    15901 caaacaaatc atttccgtct acagtaatac cgggcatgtt atatgcagat gctctcacgg
    15961 aaacatccgc aatagcttgg tgtcttttct ggcttacgga aataccatac ccgttgtttt
    16021 cacacacaaa tattaccggg agtttccaaa tacttgccaa gttcaaagct tcatggaatg
    16081 tactctggtt ggttgacgca tcaccgaaaa agcacacgca gacctggtct gttttcttgt
    16141 gttgaatagc caagcccgaa cctactgcaa tattatgacc tgcgcctaca ataccgtttg
    16201 ctcccaaaat gcccttggtt gcatccgcaa tatgcatcga gccgcctttg cctttgcaat
    16261 atccgtcttc ccttccaaaa agttcagcca tcatgtattt taattctccg cccttggcga
    16321 ttatgtgtcc gtgccctctg tgggtgctgg ttatgtagtc atcttccctt aaatttgcgc
    16381 aagcgcctgt tgcaacagct tcttcaccta tatagaggtg gacaaagccc ggaatttttc
    16441 ctgcagcaaa caattctgac gcactttttt caaactttcg aatctttagc atggtcaaat
    16501 acatttcttt caattgttcg ttagtgattt tcaaaatact acctcccatc atttttaatt
    16561 tttacggata cactcatccg tcacatcctg gccatggaaa tccgctccca tgccgatttc
    16621 aacaataaat ttttccaagg caccacccct ttaatataaa taatatttta tattataatt
    16681 tttatgacaa gcttttaaga ttctaggtct ttgtcataca cggattgtag gacagtcaaa
    16741 tgatataaaa tgcatgcctg aaattatgtc atgatgagaa aatgtatcag attgagaaaa
    16801 aacaccgaga tgtttcgatt cttgagaact gtcgtataat gtttcgcata gatgagttgt
    16861 ttatattatt gtacttttat atatatccat aatttagcat attaaacaaa aaatctcaac
    16921 aggtaattga gatttttcat gagattcttc gggctaaaac cctcacattt ttttaaatat
    16981 ttatttaatt attaagtcta agtgccctca tggcattcaa tacagctata aaggttacgc
    17041 caacatctgc aaatactgca gcccataagc ttgcaaggcc taaggtgctc aaaaccagaa
    17101 ttatcacttt taccgatatg gcaaaaataa cattttgata tgcaatcttt atagtatttt
    17161 tcgcaatttc aattgctttt gcaattttag acggctcatc cgtcataatt acaacatcag
    17221 cagcttcaat tgcagcatct gagccaagag ctcccattgc aactcccaca tcggctcttg
    17281 ccaaaacagg tgcatcatta ataccgtctc ccacgaacat taatgttgcc ttttccgatt
    17341 tttgagaaat caattcttct actttttcaa ctttgtctgc aggcaataac ccggcataaa
    17401 ccctgtcaat tcccaattcc cttgcagtat ttattgcaac cgattcactg tcaccggtaa
    17461 gcattactat atccttgatg ttatgtttcc ttaaatcact tacagcttca aaagcatcat
    17521 cctttatctt atcagcaatc agaatatacc ctgcatattc tccgtctgca gcaatatgga
    17581 ctagagttcc ttcagatgtt tctttattgt atggaatttt gaatttttcc ataagcttat
    17641 catttccggc caagacttta atattttcta ctgctgctga tacaccatgt cctgaaattt
    17701 cttccacttg agaaattttt tcaatgtcga tatctttttt gtatgcttct tttaatgaaa
    17761 gagaaatcgg gtgatttgaa taactttctg cataagctgc aagttttaaa agctcctccg
    17821 gtttaatttt ctcagaatga attttttgca ctttgaaaac acccttagtt aatgttccgg
    17881 ttttgtcaaa aacgattatt tcggttttcg ccaaggattc caggtaattg cttcctttaa
    17941 caagaatacc gtttctggaa gcacctccta ttcctccgaa gaaactcaga ggaactgaaa
    18001 ttaccaatgc acaaggacat gatacaacca aaaatgccaa tgatctgtaa atccactcat
    18061 taaatgtagc acccttaata atcaatggcg gtataattgc gagaacaact gctattacaa
    18121 ccacaactgg cgtgtaaacc cttgcaaatc ttgttatgaa tttctccgat ttggatttcc
    18181 tgctgcttgc attttctacc aattcaagta tcttatttac ggtggattcg ctatagctct
    18241 ttgtaacctt tgcgttgagc aatccattca tattaatgca accgcttaac aattcgcttc
    18301 ccgtaattac ttcccttgga agtgattcgc ctgtaagagc tgatgtgtcg agcattgagt
    18361 tcccttccac gacaataccg tcaaggggaa ccctttctcc cgcttttatt actattattt
    18421 cacctatctc cacttcttca ggaggtattg taagaacttc gccattcctt aatacatttg
    18481 cataatccgg acgaatatcc atcaaacttg aaatagatct tctggattgt cccaccgcat
    18541 agctttgaag catttcacca atctgataaa ataacattac gccgacacct tcaggagctt
    18601 cacctataaa aaacgctccg attgaagcaa cagacatcag aaaattttca tcaaaaactt
    18661 gtccgttttt aatgttttca attgctcttt ttactacatc gccaccggct aatatataac
    18721 ttattatata cattattaat tttaattggc tgtcatttat aattattgca gaaacaaaaa
    18781 atattgcact gataattaac cgccataata attttttcat aaattctcct gttaagcttc
    18841 ttcaaatact acctgcggtt catatttttt tattattttt aatgcagctt cttctatttc
    18901 aggcataaaa ccatcatctg cttcaattat cattttctct gtgaaaaaat tagatgtaac
    18961 gcttttaaca cccttaagtt tgcttatctt tctttccatt tttgctaaac aattagcgca
    19021 atccaatcct tgcaatttat acgtctttgt catatcttcc tccacatatt ggttcagttt
    19081 attcattata ttttgctttg atttatttgt tatatttttc tttgatatgc tccataccta
    19141 tttcgtatat atcccttata tgcttgtcct tcaaggagta ataaaccgtc ttgccttctt
    19201 ttctgaactt aactaaattt gcatttctta atgcacgcag ctgatgagaa atggctgact
    19261 tggtcatatt caacaaaaca gcaatatcgc aaacacacat ttcgctttca tccagcgccc
    19321 aaattattct cattcttgtg ctgtcaccca acaccttaaa gaaatccgat aaatcataaa
    19381 tatcctcatc atcgggcatc ctttttttga cctcattaac aatatctcca tgaatcactt
    19441 cacagtcgca tacaaaacct tcattagaag acatttctac ctcccgtttt cagttgaatg
    19501 attgttcaac cgtttgatta tattatagtt gaatgtctgc tcaattgtca atagttttat
    19561 cactatggtt cctctgaatc tgttccgtga gaaaaatcaa taatttccga ctgtgaaagg
    19621 attgttcccg tgtcggagaa ttctacaatt tcatcatctt ctgcaccttc cccggcaggc
    19681 attctatcaa cgtcgtcact tgatgtttct tttgttgaac ctaaattgtg atttcccgtt
    19741 tcgtcaacta cataataacg agtaaaagga gattttctgc ctttcactaa gttatacttt
    19801 tcaatcattt tttcgtcaag gggaattaat ccgttttctg tctgtaaaaa taatttttcc
    19861 attttaatct ccaataattt ttttatagta taccctttta aaataattta ataaatttga
    19921 ttttggtaaa tgaatagact taaaactata ttatgatttg tgatttagtc actgcaataa
    19981 tatttaaatc ggataaaatc atatcataaa actgaaatta ccgatttgtg acttagtcac
    20041 tgcaataata ctttaatcag gtaatataaa aatataataa gctgaaagga gattatgata
    20101 tgaatacagt aaaaattaca agcgacaact tcgagcaaga agttttaaaa tcagataaac
    20161 cggtactctt agatttctgg gcagcctggt gcggtccctg caaaatgatt tctccgataa
    20221 tagaggagat agcaaaagaa gtaaccgatg caaaaatagg caaggttaat atagatgagc
    20281 agccggaatt agcatcagca tttggcatta tgagtatacc aacattggta aaaattaaag
    20341 acgggaaagt tgttaacaaa atgattggag taagacctaa agcttcaata gtacaaatgt
    20401 tagcatcata gaattttctc ctccaaaaat ataatatttt acaccaaaaa aagaacccaa
    20461 taattaaacc ccctatatgg aacctcgcaa aaggcagcag tagctgcctt ttttgttgaa
    20521 attataaata taaattgata taatcaattg aaaacacaaa aatggagaaa atcatgagca
    20581 gtctggtttt aaaaataacg gcactaatca caatgataat cgatcattac ggagcaattt
    20641 ttcaaggtaa tataattatt tacagaataa taggaagatt agcattcccg atttattgtt
    20701 tcttattggt tgaaggatat acccacacta gcaatataaa gaaatatgca tcaagactga
    20761 tagcatttgc tatcatttcg gaaatacctt ttgatttggc atttttcggt aaaatcggtt
    20821 ttgagcatca aaatatattt tttactctct ttataggttt agctgccata tattttattg
    20881 ataacaagga aggaaaatat agcaccaata gtgcttttat tattattgca gcatctgtca
    20941 ttgcaacctt gcttagaacg gattatcaat ttataggtat tatttatatt ttagcatttt
    21001 attttactag aaatcaggca aagcacaaaa aatttctcac tataggatta attatgtttg
    21061 cagtaaatct ttcggcagga atgctgcaac aattctcttt gcttgcatta ataccaatct
    21121 atttctataa caagaaggaa ggaagaaaaa acaaaattct tcagataagt ttttacactg
    21181 cttacccctt gcatctgata atattttatc taataaatac attataaaac atagctcaaa
    21241 tattttaacc tttcacataa atcctctctc cgaacccgcc ccgggaagca gtctcatatt
    21301 caataattgt ttataataat taccagtcat gcaataattt agtttaattt ttgtaataaa
    21361 cttgtaatat atgacttttg cttcataatt gtgtaatata tttgttacat ttaccaataa
    21421 aacataatat tgagggtttc attacgaaaa gtatcgtgct ataattcgtc agtaactaaa
    21481 aataagttac acgcttcaac tgaagtgtaa ggggtaaaag aatatatata gaaggagaaa
    21541 caaaaaatga aaaagaaaat tttatcttta acattagcgc taattatagc actatcttca
    21601 acatctgcat ttgctgcaga acaagtgtat tatacaaact ttaatattta tgacttgtgt
    21661 aaaaatttag gttattatta ttttgaacct gttaatacaa acaataacat tgaaataaaa
    21721 acaaatattg aaaaagctcc tgtaaataac ggagccgaaa caactcaagc tcctgtaaat
    21781 aacggagccg aaacagctca agctccttca aatgaaagag caacaacaga agctgcaaca
    21841 actgctgctc cttcagtatc aaattatgaa agaagagtag tagagcttgt aaatgttgaa
    21901 agacagaaaa acggattagc accgcttact ttggatcctg caatatcaaa tgttgcaaga
    21961 atcaaatcaa aagatatgtc agacaacaat tattttgcac accaatctcc aacttacggt
    22021 agtgccggtg atatgctttc tcagcacgga atcagataca gtgcatgggg tgaaaatata
    22081 gcagcaggcc agagaacacc ggaagaagta gtaagtgcat ggatgaattc accgggccac
    22141 agagcaaata tacttggatc acactataca aaaataggtg ttggctatgt gaataacggt
    22201 agaccatatt ggacacaaat gtttacaaat taagcaaatg acccaaatct ttgatttggt
    22261 gtcagttgct tatcctgagc gtatgcgaag gatcccatgt cttaaaagat agattcttca
    22321 ctgcgttcag aatgacaaag aaaaatatac tctaaatcaa taacagcagg catttctaat
    22381 tgaagtgtct gctgttttta tttgaattca gtcatatttt ttctgtatct tgcgggtaca
    22441 aattgaaact gtaagttaat attttttctc tccttaattg ccaaatcggt aaagtattgt
    22501 ttccacaatt tatgtatctt tgtttcatct aaagaaaatt caacattatc aatatttatg
    22561 ttttctttaa tttcccaact gttattagcg tacacaaccg ctttttttct tttttcatca
    22621 tatataataa acttttcatg cttatatcta tctgcaaaat ggtcagccaa taagatgctt
    22681 atgtcattat cgggagaata tttagcaaac aaaattcctc ctatatcaga aaacctgagt
    22741 atgccaagca ccctgtgctc ttctcttgct acctttttgt atatttcatt aataggaagg
    22801 accttttcat gtgtatagaa gttcatggta tctctgccgt acttaaatgc aaatattaaa
    22861 aaatctaaaa tcaagttttc ctttttagaa tcatttgaaa gatagcaata ataaacatgt
    22921 atataagcct ctttagaaat ctgcctattg attgaatcgc acactatttt tgctttcgaa
    22981 atatctgttt ttacaatttc cgcctcatat acaattgatt gctgatattc agatatttca
    23041 tatataccgg tcgccttttc ttttttgtaa tgataataaa tgcaagtcat caatccttca
    23101 aatgtaccgt cgtataaata atccatgatt ataacaattc aaagatactc atttgtcttt
    23161 gaatttcctt actccttatt tctgctttta ttgcttccgg gtcaatcttt gtattgccat
    23221 agtatttacc tttaacggta ataaaatatt tagctctctt tataacagtc ctcatttttt
    23281 taagagaatc ataggaaatt tgggcatttt ttctttccct tattatcctt tgggcactgg
    23341 ttacaccaag ccccggtatc cttaaaagct cttcgtatga tgcattgttg atttctatag
    23401 gaaatttgtc tatatttctc aatgcataca tcattttcgg gtcaaattcc aaatcaagat
    23461 tttgctcaac ttcggtaaac aattcctcag atttataata ataaaaccgc atgagccagt
    23521 ctgcctgata caacctgtgc tcccttaata atggaggcat ggtacttacg gcaggcaaat
    23581 tcggtgatga cataatcgga acgtatgcag aaaaaaacac tcttttcatc ctgaatttat
    23641 catacaaatt ttgtgatgcg cttaggattg ttttatcgct gtcaggggtg gctccgatta
    23701 tcatctgtgt cgattgaccg cccggtacaa acttctgagc atttttaaaa tgactcttat
    23761 cttcaatagc acgagaaatt tcattatgta tgagagacat tggcttaaga atattctcca
    23821 tctttttttg aggtgccaat aaggataagc tttctcttgt gggaagctcg atatttacgc
    23881 tcatcctgtc tgccagcata cctgtttttt gaactaatag aggatttgct cccggaatga
    23941 ccttcacatg aacatagccc caaaaattat acttatatct taagagctca agtaccctgt
    24001 aaatattttc catggtatgg tcgggacttt tttcaacggc cgagcttaaa aataaccctt
    24061 caatataatt tcttctgtaa aattgaatgg ttagctccgc aacctcttcc ggggtaaaag
    24121 ttgctctttt gattggattt gtcaccctgt tcaaacaata tttgcaatca taaatgcagt
    24181 cgtttgtaag taaaattttt agaagtgaaa tacatctgcc gtccgtggac caggcatgac
    24241 aaatacctgc agcagaagca ttgcctattc ctcccactgt attgtttttt ctttctacac
    24301 cgcttgaaac acatgatacg tcgtattttg ccgcatcaga caaaatcctt aacttttcaa
    24361 acaaattgtc ttccatattt tcaccaagtt tttcttagtc ttctttgatt attatattac
    24421 aaagaaaaac a
//
LOCUS       DAOPUE010000020        23495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2159_length_23495_cov_10.974915, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000020 DAOPUE010000000
VERSION     DAOPUE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23495
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_23495_cov_10.974915"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..605)
                     /locus_tag="PKH42_03305"
     CDS             complement(27..605)
                     /locus_tag="PKH42_03305"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOA19489.1"
                     /translation="MFFEALILGIVIGYIRRGKLSRLSYVNFSFRFLIYISALFYLGI
                     IVINLGLYDYESFLYSTFLIGSIVLTGLFLIANISIKYMFIPLIGLALNLLSFLTNNF
                     KFPLSPDAAAKIYGAEAAELLNAGKLLFYTSSETATLSFLGNIIPIGNWFVVSIGDII
                     ASLGVVLIVQCIMADKFIQTRSKITFSKDIFR"
     gene            837..2171
                     /locus_tag="PKH42_03310"
     CDS             837..2171
                     /locus_tag="PKH42_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOA19490.1"
                     /translation="MLEEGSLWGPLVLQFILLFINAIFASAEIAVISMNDNRIAKMML
                     EGDKRAIRLSKLTDNPSQFLSIIQVAITLAALLGGAFAAENFAGRLTGLLINIGVNIP
                     YSVLNSVSVVLITLLLSYITLVMGELVPKRIAMKNAERLALSLSGLLYFISKLFAPVV
                     WLLTVSTNGILRLLGIDPNETDDEVTEEDIRMMVDVGSEKGSINENEKEMIQNVFEFD
                     NKTAEEVMTHRTEAVILWLDETEEEWDKKISESRHSRYPVCDGSADNVIGVLHVKEYY
                     TIEDKSRENIMKMAVKPAYYVPETVRTDVLFQNMKKSRNHFAVVFDEYGGMSGIITMN
                     DLLEQIVGDIDDDYLTPEEEEIKKIDDRTWRIKGSAYLDDVSKELGVSLPDEDFDTFG
                     GLVFGILGIIPEDGSTLELEEYGLKIVITEIKGRRLETATVYKNVDNDEDTN"
     gene            2198..2806
                     /locus_tag="PKH42_03315"
     CDS             2198..2806
                     /locus_tag="PKH42_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOA19491.1"
                     /translation="MNYNIKDILEIMPGFLLGISIHEFSHGLAADRLGDNTPRLQGRL
                     TLNPAKHLDPIGFLCLLVFGFGWAKPVMINPRNFKNPRRDDAIVSLAGPISNFLIAFL
                     FMGLIKLTINTSLSPTAIDVFYGTVMVNLMLMVFNLIPIPPLDGHHILGSIGGPKVWN
                     FYFKYREHLRFALLLLIIFRGIGRIIGPLIFILYRLLVSIFF"
     gene            2954..3529
                     /locus_tag="PKH42_03320"
     CDS             2954..3529
                     /locus_tag="PKH42_03320"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOA19492.1"
                     /translation="MESKSKIRKRILNVRNNMSKEDVKKNSNAIMDKITSLDIYKHSK
                     VVFIYMDFKNEVMTSNLIKRMLSEKKRVVIPYTDSINTVLIPSEITKESDLKQNSFGY
                     FEPKSILPVNIEEIDLVIVPGVVFDKNLNRIGFGKGYYDKILNRLKPSAKKVALAHDF
                     QVLEDIPAEEHDVKMDMIITEKNIYPFTGLL"
     gene            complement(3513..4232)
                     /locus_tag="PKH42_03325"
     CDS             complement(3513..4232)
                     /locus_tag="PKH42_03325"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOA19493.1"
                     /translation="MKGYVKSFLELLYPEKNTCFICNVYDSSIDDKYICSDCEKKLIK
                     IVPPLCIKCSKPIDYASDCLCPDCNVYEKSFEAAKSPFSYEGIIKECIYNFKYYNKPY
                     FYKFFGNLLLRYMNEINYTNFDFITSVPLHKSKMRTRGYNQSQLLAKYMSDKLSIPYA
                     DVLKRTVKTTKQSQQSKDERKKNLKGAFAVKYPHTNTKIKNRSVLLVDDVYTTGSTAD
                     ECSNTLLNFGVSKVYVITIAR"
     gene            complement(4306..6534)
                     /locus_tag="PKH42_03330"
     CDS             complement(4306..6534)
                     /locus_tag="PKH42_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOA19494.1"
                     /translation="MERLKGTIVETIFRNNENGYTVALLETKNELITITGVFGTDISG
                     ENIEVFGKRVHHPKYGEQFQVDIYNSILPTGLEQIENYLSSGLIKGIGRHTAKKIVEA
                     FKEATFDIIQNTPEKLTEIEGIGEKKADMIAKSFQEQADVKEIMMFLQSHNISTNYNI
                     KIYKEYGKNTINVIKENPYRLAEDIYGIGFKLADKIAASLGVEKDSPYRISSGVKYIL
                     AEYAARGHTFVPYNLLVKSSANLLETSIETIEDEIRNMAFTDKIHIETIDSETVVYYL
                     PYHTAEVNVCKNLINLAGFKTDISEEDVLMYLESDEDNSINLTEMQKEAVVQSILNGV
                     TVITGGPGTGKTTIILSIIKIFEAMNKKVLLCAPTGRAAKRITESTGREAKTIHRLLE
                     YAYGENDSNLVFNKNENSPLDCHAIIVDEMSMVDILLMNNLLKALKRGTKLIMVGDVD
                     QLPSVGAGNILSDIIKSNTIKTIRLDMIFRQSAGSMIVQNAHAVNKGMMPLLNIKNSD
                     FYFIRENNINNIAKLIVELYKERLPSKYGLNPMKDIQVLSPMKKGDAGVLELNKKIQE
                     AVNPPSKLKKEKVLGKYTFREGDKVMQIKNNYQTEWKLTGNNLVTGEGIYNGDIGNIL
                     FIDEAEQEMYVQFDDEKEVVYSFNQLDELILAYASTVHKSQGSEFPVIIMPIVWAPPM
                     LLTRNLLYTAITRAKRLVVLVGQEKYMEEMIENNKIDKRFSALDYRLKSAVETYKLLT
                     DL"
     gene            6789..8381
                     /locus_tag="PKH42_03335"
     CDS             6789..8381
                     /locus_tag="PKH42_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOA19495.1"
                     /translation="MSKESFIKGAAILSVAGLFVKILGAVYRIPLTNLIGTEGIGHYQ
                     PAYNIYNLLLVVSLSGFPTAIAKMVSERRALNNYKGAYQVYKIAGWGLFLIGLVSSIF
                     VLIFARNLVTFLGFPGSYYSMLALVPALFAVPLLSAYRGFFQGMQNMTPIALSQLIEQ
                     IFRVAVGLYLAYALAGTGLEEAAAGATFGASAGGIAALILIYIMFIFSKKSIKEEISV
                     SKNNEAEPVQYVIKSLLYIAVPITIGASIAPLMGNMDSFIVSNRLAALGYTVEEYTDM
                     FGELSGTAQTLINFPQVFSTAVAMSLVPSITEAFTKKQGIRLNETANAGVKMSLIIGL
                     PCGIGLFMLAEPIIALLYPSIGPAKHASSGALLEILSIGVIFLTLVQAFTAILQSVNK
                     QFHPVKNLALGLIVKVVLSYMLIGIPNINIKGAAISTTIAYLFVALFNWHDINKTKIK
                     IDLIKVSSRALLSTAAMAVTVWISFSVILSAIGSRNLATLGSIAIAGTVYVICLFITG
                     AITKEDLELIPKGEKLKKFVRK"
     gene            8383..9816
                     /gene="mazG"
                     /locus_tag="PKH42_03340"
     CDS             8383..9816
                     /gene="mazG"
                     /locus_tag="PKH42_03340"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOA19496.1"
                     /translation="MSIIQIIGLGAGGEDDLTVRAQKALSEKIPTFARTDRHPIVNEL
                     RKNIDIVSFDDYFEKYETFNEVYENIINTLIEKSKQYGKINYCTAGSPYYGDIVTKKL
                     INEYKKQINIIIIDGMSFLDKCLKLSGYADYNNIKILDCLEADEFSFDVNSFNIITQV
                     YDLDIASGLKIKLMEIYPEDANSVIIDVLEEKIEKVPLFMLDQEKNYGFSTYFCILPI
                     ELSRNRVYNVDNLLRIVKLLRGPDGCPWDKKQTHHSIRQHVIEEAYEVVDAIDNDDVD
                     NLVEELGDLLFQVVFHAELGSEKGYFNFSDVVTNVCKKMYFRHPHVFGDVKADDVEEA
                     LTSWEHSKLKEKNLTTYTDNLKNVPKALSPLSRSYKIQKRAAEVGFDWSDAKGAVLKI
                     KEELLEFIDEYNKGNIENMEEEFGDLLFALVNFARFEKINPDIALNKTINKFIERFEY
                     IEKNSTKDLKQMTLNEMDELWEESKFR"
     gene            9866..10144
                     /locus_tag="PKH42_03345"
     CDS             9866..10144
                     /locus_tag="PKH42_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOA19497.1"
                     /translation="MNKAELIASVAEKTGFTKKDAEKALNGFMETIKEELVAGGKVQL
                     VGFGTFEVRDRKERQGRNPRNPGETIDIPASKAPVFKAGKSLKEAVNK"
     gene            10408..11298
                     /locus_tag="PKH42_03350"
     CDS             10408..11298
                     /locus_tag="PKH42_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOA19498.1"
                     /translation="MWEVIEHALEHTFKDSFKMLPFLFAAYIIIEFIEHKSSDKLVSG
                     LQKFGVVGGAVLGGVPQCGFSVAASNLYAGRIITAGTLIAVFISTSDEAIPILLSSNG
                     NLNVILLLITVKAIFAVIAGAMADFIFKGFFNKSNNEGIASSIHHHVCTDCGCHDDPG
                     ILKPALKHTINIFIFLFITNFLINFFIEYVGEDNLSEILLHDSIFQPALAGIIGFIPN
                     CAASIILTQLFIAGSISFGSVIAGLSTGAGVGLLILFKVNRNINENLKIMLYIYVFSV
                     LAGSLIQTMGTYLTVPHGFF"
     gene            complement(11383..12090)
                     /locus_tag="PKH42_03355"
     CDS             complement(11383..12090)
                     /locus_tag="PKH42_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19499.1"
                     /translation="MLKINGLHVNYGGISAVKGISIHVPEKSIVTLIGANGAGKSTTL
                     KAIAGLVKARNGEIIFQNENILGKDTIDIVARGITMVPEGRKVFVNLSVLENLRVGAY
                     LRRDGIEDDIKRVYDLFPILKERAWQLAGTLSGGEQQMLAVGRALMARPKLIMMDEPS
                     LGLAPLIVNSIFEIIKEINKQGTTILLIEQNANKALKVADYGYVLETGNITMEGKGIE
                     LLKNEKIIEAYLGTSKK"
     gene            complement(12083..12853)
                     /locus_tag="PKH42_03360"
     CDS             complement(12083..12853)
                     /locus_tag="PKH42_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19500.1"
                     /translation="MSVLKINNVTMQFGGVTAVNNLNIEVQEGRIEALIGPNGAGKTT
                     AFNVITGVYSPTKGNVFYKNENITGLTPDKITKKGIARTFQNIRLFKNLTVLENILIA
                     NHLRINSNFLSSTLRMPWSNKEERQMREKSEMLLDKLDLLRLKNEIAHSLPYGEQRKL
                     EIARALATDADLLLLDEPAAGMNPSETMELSSFIHNIRDEFHLTIFMIEHHMDLVMEI
                     SEKIYVLDFGQLIAEGTANEVKNDQRVIEAYLGVEEDA"
     gene            complement(12850..13944)
                     /locus_tag="PKH42_03365"
     CDS             complement(12850..13944)
                     /locus_tag="PKH42_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA19501.1"
                     /translation="MMDKLNNKNNKLILNILLIIIFASIIILLDTKKIGDAYIRRILN
                     LSAIYAIVSVSMNLVNGFTGLFSLGQAGFMAIGAYTVAIFTVPLAARAKIFYITPQNP
                     VLAQIELPFVIALILGGLLAALVALLIGLPVLRLKSDYLAIATLGFSEIIRIVFTNIK
                     SITNGALGIKNIPTMPTMWVFFGIAIISIGFMWLLINSSYGRAFKAIREDEVAAEAMG
                     INLFKHKVMSFSIGAFFAGIGGGLLATLFATVDPNQFYFPVTYNFLLIIVLGGMGSIS
                     GTIIASFVVTSGLEILRFFDEPLSLFGLNIPLFRPGLRMVIFSILLMVLVLFFRNGLL
                     GQKELSWKLIFDLLSGKKPRKKTGEGGAAQ"
     gene            complement(13941..14834)
                     /locus_tag="PKH42_03370"
     CDS             complement(13941..14834)
                     /locus_tag="PKH42_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA19502.1"
                     /translation="MTGEIFMQQLANGISLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMMAAYFMVFSIITFNIPWFISIIITIIATVTLGVLVEKTAYRPLRNAPRMSIMISAI
                     GVSFLLENLATYLFTGVPRGFPDIKVLTTTMKLGSLSLTVATLITPFVTLMLLYVVLF
                     ITNKTKIGMAMRAVSKDFETAKLMGININQVISITFIIGSFLAAIGAVLWGSRYPSVM
                     PLMGVMPGLKCFIAAVLGGIGNTTGAVLGGFILGMAEIMLITFLPSLTGYRDAMAFVI
                     LIIVLLVKPTGLLGEKVTDKV"
     gene            complement(14907..16118)
                     /locus_tag="PKH42_03375"
     CDS             complement(14907..16118)
                     /locus_tag="PKH42_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA19503.1"
                     /translation="MKRILSLLLSVLMVLALFTACTPASNNGNGNEEPETGNEEEPVA
                     EDADVIKIGVFEPLTGMNGGGGLQELGGIEYANKMYPEVLGKKIQLVVVDNKSDKGEA
                     TTAVTRLIEKDKVVAIIGSYGSAVSIAAGDIIKNAQIPAMGASCTNPMVTQGNEFYFR
                     ACFLDPFQGKVMATYAIEQGAKTAAVIMQNGDDYSVGLANFFINAFKDLTGDENSIVD
                     VSEFQLNESDFNAILTNIKAKNPDVIFAPSAATTAPLIIKQARDLGIDSLIMGGDTWE
                     NPAIIDVAGEDAEGIVLSTFFDENDPATEEARKFVEGYKKELAGKEPIIPAVAALGYD
                     AYILLRDAIERAGSTDGVAIRDALKATKDFEGVTGIINFTEEGDADKNLAVIKTVKDG
                     QFVFVDSVEVK"
     gene            complement(16324..17553)
                     /gene="glyA"
                     /locus_tag="PKH42_03380"
     CDS             complement(16324..17553)
                     /gene="glyA"
                     /locus_tag="PKH42_03380"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOA19504.1"
                     /translation="MNFNLIEENDNELYRFIMEEKERQRINIELIASENFVSDTVLQA
                     MGSHLTNKYAEGYPGKRYYGGCEFVDKVENLAIERAKKIFGADHVNVQPHSGSNANFG
                     VYFAVLKPGDKILGMDLSHGGHLTHGSPVNLSGSYFNVVFYGVNRETETIDYDQVREI
                     AKKERPKLIVAGASAYPRIIDFKAFRDIADEIDSYFMVDMAHIAGLVAAGLHPNPVDY
                     ADFVTTTTHKTLRGPRGGMILCRQEFAQKIDKAIFPGTQGGPLMHIIAAKAACFKEVL
                     DDEFVLYQQQVIKNAKKLSEELMNKGFRLVSGGTDNHLMLVDVRNKNLTGKEAEKILD
                     EIHITTNKNTIPFDTQSPFITSGIRIGTPAVTTRGMKEAEMIELADIMDSALSKKENE
                     SMLKERVLKLTSKFSLE"
     gene            complement(17564..18694)
                     /gene="alr"
                     /locus_tag="PKH42_03385"
     CDS             complement(17564..18694)
                     /gene="alr"
                     /locus_tag="PKH42_03385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOA19505.1"
                     /translation="MNEKLRPAWVEINREKAIHNFLEVRRAVGPDRKICAVVKADSYG
                     MGALELSKMYLENGVDMFAVAVIGEAFELRKEIKDKDILVLGFSSEEFYDDAIENNIT
                     LTIYDYNHALRLNETAKKLNKQAKIHIKVETGMNRLGFLPTAENADKIADIAKFENIK
                     IEGAFSHQAKADEKDKTTAHMQADRFIKFIGMLEERNVQIPIKHIANSATIIELPEYY
                     FDMVRPGIILSGFYPSDEVDTEKYKFKICVTLKAKVANVKTIEAGEGVGYGHLYRAEK
                     PVVVGTIPLGYADGYSRLLSNKGYVVVKGVKCPILGKVCMDQFMVDLSNVENPQIGDL
                     AIVYGDGTDGAMTAEDVANMRGTISYEVLTNLAVRRLPKIYV"
     gene            complement(18898..21222)
                     /locus_tag="PKH42_03390"
     CDS             complement(18898..21222)
                     /locus_tag="PKH42_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19506.1"
                     /translation="MKKTLLVIIILIFVASFINIYAAVFPDIKKDPYLEEAIGVLSSY
                     KVVQGYPDNSFKPDRIVTRAEMAKVVTVAAGFNEYSKNMTSSYEDMHGHWAESYVELA
                     DVLGIVKGISPSVYGPDNLIKFEEAYTMILRLLGYTDESLMGSWPSNYREKAIELNLF
                     ENINSNTEFASRRDITIMLFNALSKNLVSVKENNTYNVTNKTLISKLGKMETRVVSIK
                     DLNIDNFDYTDYLFNKWDIYYDNYGNTVYVSNPQYNEFSGTVTSILSNRVIFVTDDYG
                     NVRVFQVPDIPIIINGERGSFNNLNESSVKIIYEEDSFKGEVIGIIAYKATDVIVVDR
                     DDLYKEGSRQFAGKNLPLSNSEVNFNKINIKGDAKSLEDIKVNDVVYFYETQNDKNSS
                     LTLNVLRSKVEGVVTSVDNVNGATFYTINNISYMTGKDYIFTEKANVNDSVTLILDKN
                     NNIIKLYINKYGKRPSTYGIVINSSSGTNGNAEARILDEYGRIRTYSLADNSSVVRVH
                     ENDSSVLKQSLIGSNDLVMFDPVSNGTLKIINLMQTRNIASPYNNQTQTLENGGYKIS
                     SDTFIVYRNNGQYQLLVPSQLGEYLEGRAVVNYMGHIDAIYLTKGIKESNVITITPDT
                     PQNYNGTIYGIIKGVTKLDDTTSHIQFFNDSNVFSVSNTSVAGKSISSMMNSYVRAVI
                     VNGMVSSIEKVTPETSRIKISQIYTNQMLIDNITYMEYAQDVKVYVCTLDSSGNISDF
                     KEGTKSDIKAGSTAQLYNLYGGFDGVIDVVFVFK"
     gene            complement(21453..>22112)
                     /locus_tag="PKH42_03395"
     CDS             complement(21453..>22112)
                     /locus_tag="PKH42_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOA19507.1"
                     /translation="LTAIINNALTKVINIFMAFANTLLSIIISVYILIDKESIINWFK
                     EFASLFIGRKAANDIVRISHILYRNVSSYISGKATASLIIAFLIYIGSKYIIKCPYPA
                     IDGMVIGITNMIPYFGTFIGSVPIVLINVLYTPQKGALLFILILILQQLESLIIDPKI
                     LGSQLSIKPLLVIISIIIGGGLFGPLGLFFAVPVAALIKSIVDVYMLKSLKNVHIIDK
                     S"
     assembly_gap    22113..22212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22665..>23495
                     /locus_tag="PKH42_03400"
     CDS             22665..>23495
                     /locus_tag="PKH42_03400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOA19508.1"
                     /translation="MKKRLLGNTGMEVSVIGFGGIPIQHVSEKEAINLILECKQQGIN
                     FIDTARVYTVSEKLIGKGLREAGRENFYIASKAMNYTYDGMKSSIEDSLNDLEIDCID
                     LYQVHNVSSKKDLDTVFSENGALRALVEAKEQGLIKHIGITGHIRELLMEAIDNYEFE
                     TLQFPFNPVEPQGTELLLKALKKGMGTIAMKPMAGGTFSYPLLSLKYIMNSGLITVAI
                     PGMDTREQVIENARAGIDDEPLTKEEQEIINKEVEILGTEFCRRCGYCKPCPEGIDIP
                     S"
BASE COUNT         7408 a         4169 c         3997 g         7821 t
ORIGIN      
        1 gcgcctaagg gcgctttttt aattatttat ctgaatatat cttttgaaaa agtaattttg
       61 cttctggttt gtataaattt atctgccata atacattgaa caatcaatac aacacccaag
      121 gaggctataa tatcgcctat acttaccacg aaccagtttc ctataggaat tatattgcct
      181 aaaaagctga gtgtggctgt ctctgaagat gtatagaaca ataatttgcc ggcatttaaa
      241 agctcggctg cttctgcacc gtatattttt gctgcagcat ccggagacag cggaaattta
      301 aaattgtttg ttaaaaacga taacaaattc aatgcaagtc ctattaatgg aataaacata
      361 tattttatgc ttatatttgc tattaaaaat aatcctgtta aaactattga gccgataaga
      421 aatgttgaat aaagaaatga ttcataatca taaaggccta aatttataac tataatgccc
      481 aagtaaaaca aagctgaaat gtaaatcaag aatctaaaac tgaaattaac atatgataat
      541 ctgcttagct ttccccttct tatatagccg ataactattc ctaatattaa agcttcaaaa
      601 aacatatttg cctccattaa atagagttat actaattcta caatatagat taaattatag
      661 caacttattt ttgacaaaaa atgctagttt ttgtataaaa aattaataat atttatttag
      721 ttatcctcag tatacattga ttttataaag tttacattaa taaatgaata ttgacggagc
      781 aaaatatcta tgttattatt cttgtaatta tttaacttaa ttaggaggaa ttaattttgt
      841 tagaagaagg ctcgttatgg ggtccattgg ttttacaatt cattttatta ttcatcaatg
      901 caatctttgc atctgctgag attgcggtaa tatcaatgaa tgacaacaga atagcaaaaa
      961 tgatgctgga aggcgataag cgtgctatta ggctttcaaa acttaccgat aacccgtctc
     1021 aatttctttc tattatccaa gttgccatca cattagcagc actattaggg ggtgcttttg
     1081 ctgcagaaaa ctttgcaggc aggctcaccg gactattaat aaatataggt gtaaatatac
     1141 cttattcagt cttaaattcc gtttctgtag tactgataac attattactg tcttatataa
     1201 cacttgtaat gggtgaactt gttccaaaaa gaattgcaat gaaaaatgct gaaagattag
     1261 cgctgtcttt gtcgggactt ttatatttta tatcaaaact gtttgctccc gttgtttggc
     1321 ttttgacagt atccaccaat ggtatattga gattattggg gattgacccc aatgaaactg
     1381 atgatgaagt aacagaagaa gatattcgga tgatggttga tgtaggaagt gaaaaaggct
     1441 caattaatga aaatgaaaaa gaaatgattc aaaatgtttt tgaatttgac aataaaacag
     1501 cagaagaagt aatgactcac agaacagaag cagtaatatt gtggctggat gaaacagagg
     1561 aagaatggga taaaaaaata tccgaaagca ggcactcaag atatcctgta tgtgacggaa
     1621 gcgcagataa tgttatcggc gttttgcatg ttaaggaata ttacacaatt gaggataaaa
     1681 gcagagaaaa tattatgaaa atggctgtaa agccggctta ctatgtacct gaaacagtga
     1741 ggacagatgt tttgttccaa aatatgaaaa agagcagaaa tcattttgcc gttgtttttg
     1801 atgaatacgg aggaatgagc ggtattatta ccatgaatga tttattggag caaatagtag
     1861 gggacataga cgatgattac ttaactcccg aagaggagga aattaaaaaa atagatgata
     1921 gaacatggag aattaaaggc tcggcatatt tggacgatgt gtctaaggaa ttaggagtaa
     1981 gtttgcccga tgaagatttt gatacattcg gaggattggt tttcggtata ttgggaatta
     2041 ttccggaaga cggaagcaca ttggaattag aggaatacgg ccttaaaatc gtcataacgg
     2101 aaattaaggg cagaagactt gaaacagcaa cagtatacaa gaatgtagac aatgatgaag
     2161 atacgaacta atcagtgtat actgataagg agggacaatg aattataaca taaaagatat
     2221 attagaaata atgccgggtt tcttgctggg aatttcaata cacgaatttt cgcatggctt
     2281 agcagcagac agattgggcg ataacacacc gcgattacag ggacgactaa ccttgaatcc
     2341 ggcaaagcat ttagacccaa taggtttttt atgtttactt gtgtttggat tcggatgggc
     2401 taagcctgta atgattaatc cgagaaattt caaaaatcca agacgagatg atgcaatcgt
     2461 gtcattggca ggccctatat cgaatttttt aattgcattt ttgtttatgg ggttaataaa
     2521 acttaccatt aacacatcac ttagcccaac cgcaattgat gttttctatg gaaccgtaat
     2581 ggtaaatctt atgttaatgg tatttaacct aatccccata cctcccttag acgggcatca
     2641 tatattggga agcataggag gaccgaaggt ttggaatttt tatttcaagt atcgtgagca
     2701 cttaagattt gcattgcttc tgttgattat attcagggga ataggaagaa taataggtcc
     2761 tcttattttt atattgtaca gattattagt ttctatattt ttttaatgcc ctaaccctat
     2821 tttagactaa agggagaaaa aaatagcgtt agatcgatca tatgcagcgc aatcccaatt
     2881 tgcacgacca aagggagtgc acaagttggt gatggagcct gctaatgact aaactttatg
     2941 agttggtgat ataatggaaa gcaaaagtaa aataagaaaa agaatactca atgtaagaaa
     3001 taatatgtct aaagaagatg ttaaaaaaaa cagcaatgcg ataatggata aaataacaag
     3061 cctggatatt tataaacata gtaaggttgt tttcatatac atggatttta aaaatgaagt
     3121 aatgacatca aatcttatta aacgtatgct ttccgaaaaa aagcgtgttg ttattccata
     3181 tacggatagt attaatactg tcttaatacc ttctgaaata accaaggaat cagatttaaa
     3241 gcaaaattct ttcggttatt ttgagccgaa aagtattcta ccggtgaaca tagaagaaat
     3301 tgacttggtt attgtacccg gagttgtatt tgataagaat ttaaacagaa tcggatttgg
     3361 taagggttat tatgacaaaa ttttaaacag gttaaaaccg tcagcaaaaa aggtagccct
     3421 agcccatgat ttccaagtgc ttgaagatat acctgcagag gagcacgatg tgaaaatgga
     3481 tatgataata accgaaaaaa atatttaccc ttttaccggg ctattgtaat tacatagact
     3541 tttgatacac cgaaattaag aagtgtatta gagcattcat cggcagtgga gcctgttgtg
     3601 tagacatcat ctaccaaaag cacactgcga tttttaattt ttgtgtttgt atgaggatat
     3661 tttacggcaa atgcaccctt caagttcttt ttcctttcat ccttgctttg ttggctttgc
     3721 ttagtggttt ttacagtcct tttcaaaacg tctgcataag gaattgaaag cttgtcactc
     3781 atatatttcg caagcagctg cgattgattg tagccacgtg tgcgcatttt agacttatgt
     3841 aggggtaccg atgttataaa atcaaagttg gtgtaattaa tttcattcat ataccttaac
     3901 aggaggttgc caaaaaactt ataaaaatag ggcttattgt aatatttgaa gttgtagata
     3961 cactctttaa taataccttc atatgagaag ggagacttag cggcttcaaa gcttttttca
     4021 tatacattgc agtcggggca aagacaatca gatgcatagt caatgggctt ggagcactta
     4081 atgcatagag gaggtacaat ttttatcagc ttcttttcgc agtcactgca aatatatttg
     4141 tcatcgatgg agctgtcata aacattacat ataaagcaag tatttttttc aggatataaa
     4201 agttctaaaa acgatttaac atatcctttc attttacccc ccaattggta tgggtacaca
     4261 cctcttgtac cggtatagtc ttattggtta gaggaatatc cccaattata aatctgtcag
     4321 aagcttataa gtttccactg cagatttcag cctgtaatca agggctgaaa aacgcttatc
     4381 tattttattg ttttcaatca tttcttccat gtatttttct tgacctacaa gaacgaccaa
     4441 tcgttttgct cttgttatgg cagtatataa taaatttctc gttagcagca ttggcggtgc
     4501 ccatactatg ggcattataa taacggggaa ttcgcttcct tgacttttat gtaccgttga
     4561 tgcataggca agtattaatt catctaattg gttaaaagaa tacaccactt ctttttcatc
     4621 atcaaattga acatacattt cttgctctgc ttcatcaata aataatatat ttcctatatc
     4681 accgttatat atcccttctc cggtgacaag gttattacct gtcaatttcc attctgtttg
     4741 gtagttgttt tttatctgca ttaccttatc gccttcgcgg aatgtatatt tgcctaatac
     4801 cttttccttt tttaacttgg aaggaggatt aactgcttcc tgaatttttt tgtttaattc
     4861 taaaacacct gcatcgcctt ttttcatggg agaaagcacc tgtatatctt tcatgggatt
     4921 caaaccgtac tttgaaggca gacgctcttt atacagttca actataagct tggcaatatt
     4981 atttatattg ttttctctta taaaataaaa atctgagttt tttatgttta ataaaggcat
     5041 cattcccttg tttacggcat gtgcgttctg aacgatcata cttcctgcag actgtctgaa
     5101 aatcatatca agtctgattg ttttgatggt attgctttta ataatgtcgg ataaaatatt
     5161 gcctgctcca acagacggca attggtctac gtctccaacc attataagct ttgttcccct
     5221 ttttaatgcc ttgagtaaat tgttcatcag cagtatatca accatggaca tttcatcaac
     5281 tataatagcg tggcaatcca ggggtgaatt ttcattttta ttaaagacta aattgctgtc
     5341 attttcgcca taagcgtatt ctaagaggcg atgaatggtt tttgcctctc ttcccgtcga
     5401 ttctgttatg cgttttgctg ctcttccggt tggagcgcat aagagtactt ttttattcat
     5461 tgcttcaaat attttaatga ttgataatat aattgttgtt ttaccggttc cgggtcctcc
     5521 ggttattacc gtaacaccgt ttaatatgga ttgcacaact gcttccttct gcatctcggt
     5581 gagattaatt gaattatctt catcagattc caaatacata agaacatctt cttcagaaat
     5641 atcagttttg aagccggcta aatttattaa atttttacat acatttactt cagcggtgtg
     5701 atagggcaga taatatacca cggtttcaga gtcaattgtt tctatatgta ttttatcggt
     5761 aaatgccata ttccttattt catcttctat agtttcaatt gaagtttcaa gtaaattggc
     5821 agagcttttt accaataaat tgtaaggtac aaaagtgtga cctcttgctg catattctgc
     5881 cagtatatac ttaactcccg atgatattct gtagggcgag tccttctcaa ctcctaagga
     5941 tgcagctatt ttatcggcta acttaaaacc tatcccataa atatcttcag ctaacctgta
     6001 tggattttct tttattacat ttatagtatt tttaccatat tccttataaa tttttatgtt
     6061 gtagttagtg ctgatattgt ggctttgcag aaacatcata atttctttga catctgcctg
     6121 ctcctgaaag ctttttgcaa tcatatcggc ttttttctca ccgattcctt ctatttcagt
     6181 taatttttcc ggagtgtttt gaataatgtc aaaggttgct tctttaaatg cttctacaat
     6241 tttttttgcg gtatgtctgc ctataccttt tattaaacct gaggaaagat agttttctat
     6301 ttgctccaag ccggtgggaa gtatggaatt atatatgtca acttgaaatt gctcaccata
     6361 ttttggatgg tgaactcttt ttccaaacac ttcaatattt tcgccggata tatcggtacc
     6421 aaatactcct gttatggtta ttaattcatt ttttgtttcc aacaaagcaa ccgtatatcc
     6481 gttttcatta tttctaaaaa tagtttcaac tattgtaccc ttcagtcttt ccatcaaatt
     6541 ctccttaagg aattcccccg aactgtaata ataaaattat accataaaaa tagttacaag
     6601 gtataaaaag ttgctattta aagaagctaa aaataataaa aatatctgct gattcgcatt
     6661 tttctcttta aaagaagaat aaactgtgat aaactaaatt tcagcgaaaa aggtatgggg
     6721 acacaccggg atagtctctg cgggaagaag gaatgtccaa aatatattgt tgtaagaaag
     6781 gaagaaacat gtctaaggaa agttttatta aaggtgctgc tatactgagc gtagcgggat
     6841 tatttgtaaa aatattaggg gcagtgtaca ggatacctct gacaaattta ataggtactg
     6901 aaggcatagg tcactatcaa ccggcttata acatatataa tcttttgctt gttgtgtcac
     6961 tgtccggatt ccccacagca attgctaaga tggtttccga aagaagagct ctaaataatt
     7021 acaaaggagc atatcaggtt tataaaatag caggttgggg tttattttta ataggacttg
     7081 tttcatctat ttttgtgctt atatttgccc gtaatcttgt tacgtttctt ggttttcccg
     7141 gctcatacta ttcaatgttg gcactggttc ctgcattgtt tgcagtacct ttgctatccg
     7201 cctacagagg attcttccaa ggaatgcaga acatgactcc cattgccttg tcacagctca
     7261 ttgagcaaat ttttagagtt gcagttggtt tatacctggc atatgcattg gcaggtacag
     7321 gacttgaaga agctgccgca ggtgctacct tcggtgcatc cgcaggaggt attgcagctc
     7381 ttatattgat ttatattatg tttatcttca gcaaaaaaag tataaaagaa gaaatttctg
     7441 tatcaaagaa taatgaagca gaaccggtgc aatatgtaat taaaagtctt ttatacattg
     7501 cagttcccat aaccataggt gcttcaattg caccgcttat gggaaacatg gattcattta
     7561 tagtttcaaa tcgacttgca gctctaggat acactgttga agaatataca gacatgtttg
     7621 gtgaattaag cggaacagct caaaccttaa taaatttccc ccaggttttt tcaacagccg
     7681 tggcaatgag cttagttcct tcaattactg aagcatttac gaaaaaacaa ggtataaggt
     7741 taaatgaaac tgctaatgcc ggcgtaaaaa tgtcattaat tataggactt ccatgcggta
     7801 taggcttatt tatgcttgct gagcctataa ttgctctgct ttatccgtct attggacctg
     7861 caaagcatgc aagttccgga gcacttttgg aaatactgtc aataggagtg attttcttaa
     7921 ctttggttca agcatttaca gcaatacttc agtctgtaaa caaacaattt catcctgtaa
     7981 aaaacttagc attgggcttg attgttaaag ttgttttatc ctacatgctt atcggcattc
     8041 caaatattaa tattaaagga gcagcaataa gcacaactat tgcatattta tttgttgctc
     8101 tgtttaactg gcatgatata aataaaacta aaataaaaat agatttaatt aaagtttctt
     8161 caagagcact tttatccact gcggcaatgg cagtgactgt atggatatcc ttcagtgtta
     8221 tattatcagc tataggaagt agaaacttag caacattagg ctccatagct atagcgggaa
     8281 cagtatatgt gatttgtttg tttattaccg gagcaattac aaaggaagac cttgaattga
     8341 taccaaaagg agaaaaacta aaaaaatttg tgaggaaata atatgagcat aattcaaata
     8401 atcggattgg gagcaggcgg tgaagatgac ttaactgtaa gagctcaaaa ggctttatca
     8461 gaaaaaatac ctacttttgc aagaactgat aggcacccta tagtgaatga attaagaaaa
     8521 aatatagata ttgtaagttt tgatgattat ttcgaaaaat atgaaacctt taacgaagtt
     8581 tacgagaata taataaatac acttatagag aagtcaaaac aatacgggaa aattaactac
     8641 tgtacagcag gaagccctta ttacggcgat atagtaacaa aaaagctgat taatgaatat
     8701 aaaaagcaaa ttaatataat aataattgat ggaatgagtt ttcttgacaa atgtttaaaa
     8761 ctttcgggat atgccgatta taataatatt aaaattttgg attgtcttga agcagatgaa
     8821 ttctcatttg atgttaattc atttaatatt attactcaag tttatgattt ggacattgca
     8881 tcgggtttga aaatcaaact tatggaaatc tatcctgaag atgcaaattc cgtcattatt
     8941 gatgtattgg aagaaaaaat agaaaaagta ccgcttttta tgctcgatca agaaaaaaat
     9001 tatggatttt caacatattt ttgcattctg cctattgaat tatctagaaa tagagtgtat
     9061 aatgtagata atctgttaag aattgtaaaa ttattgaggg gtcccgatgg gtgcccatgg
     9121 gacaaaaaac agacacatca ttcaataaga cagcatgtta ttgaagaagc ttatgaagtt
     9181 gtggatgcaa ttgataatga tgatgtagac aatttagttg aggaactggg ggatttattg
     9241 ttccaggttg tatttcatgc cgagttgggc agtgaaaaag gttattttaa ctttagcgat
     9301 gttgtaacca atgtctgtaa aaagatgtat ttcagacatc cccatgtatt tggagatgtg
     9361 aaggccgatg atgtagaaga agcattaaca agctgggagc attccaagct gaaggagaag
     9421 aatttaacaa catatacaga taatttgaag aatgtcccca aggcattatc acctttaagc
     9481 agaagttaca aaattcaaaa gagagctgcg gaagttggat tcgattggtc tgatgcaaaa
     9541 ggggcggtat tgaaaattaa agaagaactc ttggaattca ttgatgaata caataaaggc
     9601 aatatagaaa atatggaaga agagttcggc gacttgttgt ttgcacttgt taattttgca
     9661 aggtttgaga aaattaatcc tgatattgct ctaaataaaa caatcaataa gtttattgag
     9721 cgttttgagt acatagagaa aaattcgacc aaggatttaa agcaaatgac tttgaacgaa
     9781 atggatgagt tgtgggaaga atcaaagttc cgataggaat ttagataaag taaaataaat
     9841 tacattttta ttaaggagga tttttatgaa taaagctgaa ttaattgcca gtgttgctga
     9901 aaaaaccggt ttcacaaaga aagatgcaga aaaagcatta aacggattta tggaaacaat
     9961 aaaagaagaa ctggtagcag gcggaaaggt acaattagtt gggtttggaa cttttgaagt
    10021 aagagacaga aaagaaagac aaggaagaaa tccaagaaat cctggcgaaa caatagatat
    10081 tccggcatca aaagcaccgg tgttcaaagc aggaaaatca ttaaaagaag cagttaataa
    10141 atagacacaa attaagggga tatcgaagat atcccctatt ctattgacaa acttttgtat
    10201 gccctaaccc tatattaaat ttggggacat tcatcttaac atcagaagct atccctgaat
    10261 agggaggtgt gtccccatac cttatatata gggttagggc atcttttgaa tattaagcat
    10321 gacggtcttc gcctgggtta ttattgtagt attgacaaat aaataggtgc acatatataa
    10381 tcatactata ttttaaccgg aggaaatatg tgggaagtaa ttgaacatgc acttgaacat
    10441 acatttaagg attcattcaa aatgctgccc tttttatttg cagcatatat tataattgaa
    10501 tttatagagc ataaatcatc agataagctg gtaagtggcc ttcagaagtt tggggttgtc
    10561 gggggagctg tgcttggagg tgttccccag tgcggatttt cggtagctgc atcaaattta
    10621 tatgcaggga gaattataac tgcaggaaca ttgatagctg tattcatatc cacatccgat
    10681 gaagcaatac cgatattatt atcaagtaac ggtaatttaa atgttatatt gttgcttata
    10741 accgtaaaag caatatttgc tgttatagcc ggagctatgg cggatttcat ttttaaaggt
    10801 ttttttaata aaagcaataa tgaagggatt gcaagttcta ttcatcacca tgtttgcacc
    10861 gactgcggat gccatgacga cccgggtata ttaaagccag cattgaagca tacaatcaat
    10921 atatttatat ttttgtttat tactaatttt cttataaatt ttttcataga gtatgtaggc
    10981 gaggataatt tatctgaaat tttactacat gacagtattt ttcagcctgc cttggcaggt
    11041 ataataggat ttataccaaa ttgcgccgca tcaattatac tcactcagct gtttatcgca
    11101 ggaagcataa gctttggctc ggtaattgcc ggcctctcta cgggagcagg agtagggttg
    11161 ttgattttat tcaaggtaaa tcgaaatatt aatgaaaacc tcaagattat gttgtatata
    11221 tatgtttttt ctgtattggc agggtctttg attcaaacaa tggggacgta ccttactgtc
    11281 ccacatgggt ttttttgatt caggaaacga cgtggaaatg gggacgtatc ttggatatta
    11341 caaaatcggg acagtaaggt agttccccac tgtcccgttt ctttattttt ttgatgttcc
    11401 taaatatgct tctataattt tctcgttttt caggagctca attccttttc cttccatggt
    11461 tatattgccc gtttccaaga cataaccgta atctgcaacc ttcagtgcct tgtttgcatt
    11521 ttgctcgatt aacaaaattg tagttccttg tttgtttatt tctttaataa tttcaaatat
    11581 tgaattaact attaagggag ctaatcccaa ggaaggctca tccatcatta tcagcttagg
    11641 ccttgccatt aaggctcttc ctactgccag catctgctgc tcgcctccag ataaggtacc
    11701 tgccaattgc caagctcttt ctttgagtat tggaaacaaa tcataaactc tttttatatc
    11761 atcttcaatt ccgtctcttc tcaagtaggc accgactctt aggttttcca gcaccgaaag
    11821 attcacaaag acttttcttc cttccggaac catggtaatt cctcttgcta caatatctat
    11881 tgtatctttt ccgagtatgt tttcattttg aaaaataatt tcgccgttcc ttgctttaac
    11941 gagtcctgct atggctttaa gggtggtgct tttaccggca ccgttggctc ctattaatgt
    12001 tacgattgat ttttccggaa catgtatgct tataccctta actgcggaaa ttcccccgta
    12061 atttacatgt agtccattaa ttttaagcat cttcttccac ccccaagtaa gcctctataa
    12121 ctctttggtc atttttaact tcatttgccg ttccttctgc tataagctgc ccgaagtcta
    12181 aaacgtagat tttttcagat atttccataa ccaggtccat atgatgctca atcataaaaa
    12241 tcgtaagatg aaattcatct cttatattat gaatgaatga tgacaactcc attgtttccg
    12301 agggattcat acctgcagcc ggctcatcca agagcagaag gtcggcatca gttgcaagag
    12361 ctctggctat ttccaatttt cgctgctcac catatggaag agaatgagca atttcatttt
    12421 tcaatcttaa caaatccaat ttgtctagaa gcatctccga tttttcacgc atttgtcttt
    12481 cttctttatt agaccaaggc attctaagtg tgctggacag gaaattgctg tttattctta
    12541 aatgattggc aatcagtata ttttcaagca cggttaaatt tttgaataat ctgatattct
    12601 gaaatgtgcg agctatacct tttttggtta ttttatcagg tgtaaggcct gtgatgtttt
    12661 catttttata aaatacattt cctttagttg gagaatatac accggtaata acattaaagg
    12721 cggttgtttt tcctgcaccg ttgggaccta tcaaggcttc aattcttccc tcttgaacct
    12781 caatattaag gttgttgaca gcagtaactc caccaaattg catagttaca ttgtttatct
    12841 ttaaaacact cattgtgcag cacctccttc accggtcttt tttcgtggtt ttttcccgga
    12901 aagcaggtca aaaatcaact tccaggacaa ttccttctgt cctaacaggc cgtttctgaa
    12961 gaagagcacc aacaccatca ataatattga gaatattacc attcttaaac cgggacggaa
    13021 taatggaata tttaatccaa acaatgatag gggttcatcg aaaaatctta gtatttcaag
    13081 tcctgacgta actacaaagg atgctatgat cgtacctgat atacttccca tacctcccaa
    13141 tacgataatc aaaaggaagt tgtaggtaac agggaaataa aattgattag ggtctaccgt
    13201 agcaaacagt gtagccaata gtccgcctcc gattcctgca aaaaatgctc ctatgctgaa
    13261 tgacattact ttatgtttaa acaaattgat acccatggct tctgctgcca cttcatcctc
    13321 tcttatcgct ttaaaagccc tgccgtagct ggagtttatt aacagccaca taaaaccgat
    13381 tgaaattatg gctattccaa agaaaaccca catggtaggc attgtaggta tatttttaat
    13441 tcccaatgca ccgttggtaa ttgatttaat gtttgtaaaa acaatacgga ttatttccga
    13501 aaatcctaat gttgctattg ctaaatagtc gctctttaac ctgagaacgg gtaaacctat
    13561 taaaagtgct accaaggctg ctaaaagacc tcccagtata agtgcaataa caaatggcag
    13621 ctcaatttgt gccaacacag gattttgagg agtaatataa aatattttag ctctggcggc
    13681 taagggaacg gtaaatattg caaccgtgta agcaccgatt gccataaagc ctgcctgccc
    13741 caaggagaat aatcccgtaa aaccgttgac caagttcatt gaaacgctca ctattgcata
    13801 tattgcagat aaatttaaaa tacgtcttat ataggcatca ccgatttttt ttgtatctaa
    13861 aagaattatt atcgatgcaa atataataat aagcaatata ttcaaaatta atttattatt
    13921 tttattatta agtttatcca tcatacctta tccgtcacct tctctccaag aagccctgta
    13981 ggtttaacca acaacacaat tattaatata acaaatgcca ttgcatctct gtaaccggtt
    14041 agggatggca gaaatgtaat aagcataatt tcagccatac ctagtataaa tcctcctaat
    14101 acagctcctg tagtatttcc gataccgccc aagactgctg ctatgaaaca cttaagtccg
    14161 ggcataacac ccatgagcgg cattacggaa ggatatcttg aaccccacag tactgctcca
    14221 atcgccgcta aaaatgaacc tattataaaa gttatggata ttacttgatt tatatttatt
    14281 cccattagtt tggctgtttc aaaatccttt gaaacggccc tcattgccat accgattttt
    14341 gttttatttg ttataaacaa tacaacatat aaaagcataa gcgtaacaaa aggtgtaatc
    14401 aatgtggcaa cggttaatga aagacttcct aatttcattg ttgtagttaa aacttttatg
    14461 tcgggaaacc ctctgggaac acctgtaaac aaataagttg ctaagttttc caacagaaat
    14521 gatactccta tggcagaaat cataatggac atacgaggag catttcttaa tggtctataa
    14581 gctgtttttt caactaatac tccaagggta accgttgcta ttattgtaat tataattgat
    14641 ataaaccatg gtatattaaa agtaataatg ctaaacacca taaaatacgc tgccatcata
    14701 aaaatatccc catgggcaaa attaataagt cttagaattc cataaaccat cgtataacct
    14761 attgccaaca gggcatacaa gcttcctaat gaaatgccgt tggcaagctg ctgcataaaa
    14821 atttcaccag tcatattatt cctccaaata tctgttaaaa ctgtaaaagg gcgaacgaac
    14881 agtccgccct aatagttcta tatcagttat tttacttcta ctgaatctac aaacacaaat
    14941 tgtccatcct taactgtctt aataacagca agattcttat cagcatctcc ttcttcagtg
    15001 aagttaataa ttcctgttac gccttcaaaa tcttttgttg cttttaatgc atctcttata
    15061 gcaacgccat cagttgaacc tgctctttct attgcatctc tgagcaatat atatgcatca
    15121 tagcctaatg ctgcaactgc aggaattata ggctctttac ctgctaattc ttttttgtag
    15181 ccttcaacaa atttcctggc ttcttccgta gcagggtcat tttcatcaaa gaaagtcgaa
    15241 agaactatac cttcggcatc ttctccggca acatcaataa tggcaggatt ttcccatgta
    15301 tctccgccca ttatcaagct gtcgattcca aggtctcttg cttgtttaat tattaatgga
    15361 gccgtagttg ctgcacttgg agcaaatatt acgtcaggat tctttgcctt gatatttgta
    15421 agaatggcat taaagtcaga ttcattcaac tggaattccg aaacatcaac tatactgttc
    15481 tcgtctccgg ttaaatcttt aaatgcatta ataaagaagt ttgcaagacc taccgagtag
    15541 tcatctccat tttgcatgat aacagctgct gtctttgctc cctgctcaat tgcatatgta
    15601 gccataacct taccttggaa ggggtcaagg aagcatgcac ggaagtaaaa ttcattgccc
    15661 tgtgttacca tggggtttgt acaagaagca cccattgcag gaatctgtgc attcttaata
    15721 atatcaccgg ctgcaataga aacagctgag ccgtaagaac caattattgc aacaaccttg
    15781 tccttttcaa taagacgtgt tactgcggta gttgcttcgc ctttgtcact tttgttgtca
    15841 actacaacta attgaatttt tttgcctaat acttcaggat acattttgtt tgcatattcg
    15901 atgcctccaa gttcctgaag accaccgccg ccattcatac ctgtaagcgg ttcaaacaca
    15961 ccgattttaa taacatctgc atcctctgca accggttctt cctcatttcc tgtttccggc
    16021 tcttcatttc cattaccgtt attgctagcc ggggtacaag ctgtaaacaa ggcaagaacc
    16081 atcaatacac ttaataacaa agataaaatt cttttcatat actctctcct cattattata
    16141 aatttctatg attataacat ttaaaaaata ttattgcaag aaaattttcc gcgctatgta
    16201 aacagtcttg cacattttac atacaaacag cataatttgt atgtaaaata aaaactatgc
    16261 tgctatatga aaaaacagtc agattgtcat tactgatact attatattaa tgggttgtct
    16321 gttttattct aaagagaatt ttgaagtcag ttttaatacg cgttctttca acatactttc
    16381 attttctttc ttagatagtg ctgaatccat aatatctgct aattcaatca tttcagcttc
    16441 tttcataccc cttgtggtaa ctgcgggagt tcctattctt attccgcttg taataaaggg
    16501 gctttgagta tcaaatggaa tagtattttt atttgtagta atatgaattt catcaagtat
    16561 cttttctgcc tctttgcctg ttaagttctt gttcctgaca tcaaccaaca tcagatgatt
    16621 gtcagtaccg ccggatacta atctaaatcc cttattcata agttcttcgg ataacttttt
    16681 cgcatttttt ataacttgct gctgatacaa tacgaattca tcatccaaga cttctttaaa
    16741 acatgcggct ttagcggcaa taatgtgcat taaaggacct ccctgtgttc ccggaaatat
    16801 tgccttatca attttttgtg cgaattcttg cctgcacaaa atcattccgc ctcttggacc
    16861 tctcaaggtt ttgtgagtgg ttgttgtaac aaagtctgca tagtcaacgg gattaggatg
    16921 cagtcctgca gctacaagtc ctgctatatg agccatatca accataaagt aagagtcgat
    16981 ttcatctgct atatccctga atgctttaaa gtcaataatt ctcggataag cacttgctcc
    17041 tgccactatt aatttaggcc tttctttttt tgctatttct cttacctgat cataatcaat
    17101 ggtttcagtt tccctgttta ctccataaaa cactacattg aagtatgagc cggataaatt
    17161 tacgggactt ccgtgagtga gatgacctcc gtgagatagg tccataccta atattttatc
    17221 tcccggcttc agtactgcaa agtatactcc aaagtttgca tttgagcctg aatggggttg
    17281 aacatttaca tggtctgcac caaatatttt cttagccctt tctattgcaa ggttttctac
    17341 cttatcaaca aattcgcatc ctccgtaata ccttttgccc ggataccctt cagcatattt
    17401 attggttaaa tgacttccca ttgcctgcaa aaccgtatca gaaacaaaat tttccgatgc
    17461 tattaattca atgttaattc tttgtctttc cttttcttcc attataaaac gatacaactc
    17521 attatcattt tcttcaatca aattaaagtt catatagtac tccttaaacg tagattttcg
    17581 gaagtcttct tacggctaaa tttgtcagta cttcataaga aatagtacct ctcatgttgg
    17641 ctacatcctc cgctgtcatt gctccatcag taccgtcacc gtatactata gccaaatctc
    17701 ctatctgtgg gttttccaca ttagacaaat ccaccataaa ttggtccata catacttttc
    17761 ccaaaatagg gcattttact ccttttacaa ccacatatcc cttgtttgac agcaatcttg
    17821 aatatccgtc agcatatcct aacggaattg tgcctactac caccggcttc tctgcacggt
    17881 acaaatgtcc ataaccgaca ccttcgccgg cttcaattgt ttttacgttt gcaacctttg
    17941 ctttcaatgt aacgcaaatt ttaaatttat atttttcggt atccacttcg tcagaaggat
    18001 aaaatcctga taaaataatg cccggtctta ccatatcaaa ataatactca ggcaattcta
    18061 ttatagtagc actgtttgca atatgtttta taggtatttg cacatttctt tcttccagca
    18121 tacctataaa tttaatgaat ctgtcagcct gcatgtgagc ggttgttttg tccttttcat
    18181 cggccttagc ttgatgtgaa aatgcacctt caattttaat attttcaaat ttagcaatat
    18241 cagctatctt atccgcattc tctgccgtag gcaaaaagcc taatctgttc atgcctgttt
    18301 caacttttat gtgaattttt gcttgcttat ttaatttttt tgctgtttca tttaatctta
    18361 atgcatgatt atagtcataa atagtcaatg taatattatt ttctattgca tcatcgtaaa
    18421 attcctctga tgaaaaacct agtaccagaa tgtctttatc ttttatttct tttctcagtt
    18481 caaaagcttc tccgattact gccacggcaa acatatctac gccattttca agatacattt
    18541 ttgataattc caaagctccc attccatagc tgtctgcttt tacaacggca catattttcc
    18601 tgtcaggtcc tactgctctt cttacctcca aaaagttatg aatggctttt tctctgttta
    18661 tttcgaccca ggcaggcctc agcttttcgt tcatttaatt gctcctcctt tagtggtatg
    18721 gagaacacct ctatgataac ctttgcaggt aagctttgag gaatattccc caacttgtat
    18781 tattaataaa attatagcaa accagagtca attaatcaac aaaaaaagca tacgccctca
    18841 gtttgatgac aaagtataca atcttgtcat tctgaaggcg cagcctgaag aaacttatta
    18901 tttaaacaca aatacaacgt caatcactcc gtcaaatccg ccgtacaggt tataaagctg
    18961 ggctgttgag ccggctttaa tatctgattt agtaccttct ttaaaatcgc ttatatttcc
    19021 cgaactgtcc aaggtacaaa catagacttt tacatcttgt gcatattcca tatacgttat
    19081 attgtcaatc aacatttgat tggtataaat ttgagatatt tttattcttg atgtttcagg
    19141 agttactttc tcaatgcttg aaaccatacc gttaacaatt actgctctta cataggagtt
    19201 catcattgat gaaatacttt tgcccgcaac agatgtattt gaaacagaaa atacgttgct
    19261 gtcgttaaaa aactgtatat ggcttgtagt atcatctaat tttgtaacac cctttattat
    19321 gccgtatatg gttccgttgt agttttgtgg tgtatccggt gttattgtta ttacatttga
    19381 ttcttttatt cccttagtaa gataaatggc atcaatatgt cccatataat taacaactgc
    19441 tcttccttcc aggtattctc ccagctggga aggtactaac aattggtatt gaccgttgtt
    19501 tctgtaaaca ataaatgtat ctgatgatat tttataaccg ccgttttcta aggtttgcgt
    19561 ttgattattg tatggacttg caatatttct ggtttgcatt aaatttatta tttttaatgt
    19621 cccgtttgac acagggtcaa acataactaa atcattgctt cctattaatg attgctttaa
    19681 aacacttgaa tcattttcat gcactcttac aacacttgag ttgtccgcta aggaatatgt
    19741 tctaatcctt ccgtattcat caagtattcg cgcttcggca ttaccgtttg tgccgcttga
    19801 cgagttgatt acaatgccgt aagtcgatgg ccttttgccg tatttattta tatacagctt
    19861 tataatatta ttgtttttat ccaatattaa tgtaactgaa tcatttacat ttgccttctc
    19921 tgtaaaaata taatcttttc ctgtcatata gcttatattg tttatggtat aaaatgtagc
    19981 accatttaca ttgtctacac ttgtaacaac gccttcaacc ttagacctta aaacattaag
    20041 tgtcaatgag gagtttttat cattttgtgt ttcataaaag tacaccacgt cgtttacttt
    20101 tatatcctcc aaagattttg cgtcgccttt aatattaatt ttattaaagt taacttccga
    20161 attgctcaag ggcaggtttt tacctgcaaa ctgtctgctt ccttccttat acaagtcatc
    20221 cctgtcgaca actataacat cagttgcttt ataagctatg atacctataa cttctccttt
    20281 gaaagaatct tcttcataga tgatttttac gctggattca tttaaattat taaagcttcc
    20341 tctttctccg ttaattataa tgggaatatc aggaacctga aaaactctca cattgccgta
    20401 atcatcagtt acaaagatta ctctgtttga aagtatgctt gttacagttc ccgaaaattc
    20461 attatattgg gggttgctta catagacggt atttccatag ttatcataat aaatatccca
    20521 cttattgaac aaataatccg tatagtcaaa attatcaata tttaagtctt tgattgatac
    20581 tactcttgtt tccattttgc ctaactttga aataagtgtt ttgtttgtaa cgttgtaagt
    20641 attgttttcc tttaccgaaa ctaagttttt gctaagtgca ttaaataaca ttattgtaat
    20701 atctcttcga gatgcaaatt cggtatttga attaatattt tcaaataaat tcagttctat
    20761 tgctttttca cgataattag aaggccagct tcccatcagt gattcatcgg tatatcctaa
    20821 tagccttaaa atcattgtat aggcttcttc aaactttatc aggttatcag gtccgtagac
    20881 tgatggagaa attcctttaa caatacctaa tacatcagcc agctcaacat aactttcagc
    20941 ccaatgccca tgcatatctt cataactgga agtcatattt ttggagtatt cattaaatcc
    21001 tgccgctaca gttacaacct ttgccatctc tgcacgagta actattctat caggcttaaa
    21061 gctgttatcc ggatagcctt gtacaacctt ataacttgac aatacgccta ttgcttcttc
    21121 taaataagga tcttttttta tatcaggaaa gacagcagca tatatattta taaaagatgc
    21181 aacaaatatt aaaattatta tcaccaatag tgtttttttc atataatcaa tccttaaagc
    21241 attcaattaa aaaaatacta tgcatagtaa tagtatttct ttaatattat tatacattat
    21301 atttatttaa ttagtgttac attaacatta caatgctttt ttctgcttta aaaccagact
    21361 gtttttcaac tttatttcat aatacttata cacatgtttt atggcttttg ggcgacttgt
    21421 caacagtttt atccacaaac tcacaaatta tatcaagatt tatcaataat gtgaacattt
    21481 tttagggatt tcaacatata gacatcaact attgatttaa tcaatgctgc aacaggaact
    21541 gcaaaaaaaa gacctaaggg accaaacaat cctcctccga ttattatcga aataattact
    21601 aataagggct ttattgacaa ctgactgcct aaaattttag ggtctattat aagactctcc
    21661 agttgctgta atattaggat taaaataaac aaaagagccc ctttttgcgg agtatataaa
    21721 acatttatca acacaatcgg aacactccct ataaatgtgc caaaataagg aatcatattg
    21781 gtaattccta taaccattcc atcaattgca ggataaggac attttataat atattttgag
    21841 cctatataaa ttaaaaatgc aataatgaga gaagcagttg cttttcccga tatgtagctt
    21901 gaaacattgc gatacaggat gtggcttatc ctaacaatat catttgctgc ttttctgcct
    21961 ataaacaggc tggcaaattc tttaaaccaa tttataatac tttctttatc aattaaaata
    22021 tatacagaaa taattattga caacaatgta tttgcaaatg ccatgaatat gttgatgact
    22081 ttagtgagag cattatttat tattgctgtc agnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22201 nnnnnnnnnn nntttttttt caaaaaaaac caagacagga tttaagattg aagccagaag
    22261 tatccctgaa aatatgggaa agcatacttg gattatctta gacagaccgt tctctaaata
    22321 aataaatctg aaaactgcaa tataaaatat aaaaacaggt aaaataataa gactattctt
    22381 tttcaaatta tcacctacac agaaagaagt gtaccttacc ccctattatt gcgtcttatt
    22441 attcatatta aaatttttca aaaattatgt ataaataatg aatttctgta aataattatg
    22501 ttgataatac tttttttatc ttcttccaaa ttatataaac atcccgcgaa acaaacattg
    22561 cgggatgtcc tttttgtatg ccctataact ccccttgtct attctccatt gactcaaata
    22621 aatttctaat atataattaa cccataaaaa caatgaggtg gcatatgaaa aaaagattgt
    22681 tgggaaatac gggtatggaa gtgtctgtaa tagggtttgg aggaattccg atacaacacg
    22741 tcagtgaaaa agaagcaatt aacttaatac ttgaatgcaa gcaacaaggg attaatttta
    22801 ttgatacggc aagagtttat acagtcagtg aaaaattaat aggtaaaggt cttagggaag
    22861 caggtcgtga gaatttttat attgcctcta aggcaatgaa ctatacctac gatggaatga
    22921 aaagctcaat tgaagacagt ttaaatgact tggaaataga ctgtatagat ttatatcagg
    22981 ttcacaacgt aagctctaaa aaggatttag ataccgtttt ttccgagaat ggagcactga
    23041 gagcattagt agaagcgaaa gagcaaggat taataaaaca cataggaata acaggtcata
    23101 taagagaact tcttatggaa gcaattgata attatgaatt tgaaacttta caattccctt
    23161 ttaatcccgt agagccgcag ggaacggagc tattgttaaa agcattgaaa aaagggatgg
    23221 gaacaattgc aatgaagccg atggcaggcg gtacattcag ttatccattg ttatctttga
    23281 aatatattat gaattcaggt cttataacag tagcaatacc cggtatggac acaagagaac
    23341 aggttattga aaatgcaagg gcaggaatag atgacgagcc tttaacaaag gaagaacaag
    23401 agataataaa caaggaagtt gaaatattag gaactgaatt ttgcaggcga tgcggttatt
    23461 gtaagccttg tcccgaagga atagacatac ccagc
//
LOCUS       DAOPUE010000021        23134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2207_length_23134_cov_9.752026, whole genome shotgun sequence.
ACCESSION   DAOPUE010000021 DAOPUE010000000
VERSION     DAOPUE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 23134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23134
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_23134_cov_9.752026"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..884
                     /gene="thyA"
                     /locus_tag="PKH42_03405"
     CDS             57..884
                     /gene="thyA"
                     /locus_tag="PKH42_03405"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOA19509.1"
                     /translation="MSTADKIFKEMCKDILTKGVWDTDYDVRPRWEDGTPAHTIKKFG
                     IVNRYNLQESFPILTLRKTNFRAAVDELLWIWQKKSNNVNDLNSRIWDSWADETGSIG
                     KAYGYQLGIKHKYKEGEFDQVDRVLYDLKNNPASRRILTNIYNFQDLHEMHLYPCAYS
                     MTFNVSGNKLNAILNQRSQDVLAANNWNVVQYSILVHMMAQVSGLVAGELVHVIADAH
                     IYDKHVPIIEELIEMEEHEPPTLEIDNIDDFYKFTVDSFRLINYQYNPFKVKVPIAV"
     gene            895..1416
                     /locus_tag="PKH42_03410"
     CDS             895..1416
                     /locus_tag="PKH42_03410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOA19510.1"
                     /translation="MKLIVAVARDWGIGNKGELLFNVPEDMEFFKKTTLNKVVVMGRK
                     TLLSLPGGTPLKNRTNIVMTSDVNFKKEGCIIVNSKEALFEELKKYESDDVFLIGGGI
                     LYKELYPYCSEAYITKFDAILEADTYLHNFDEDKDWLLTYASEIHEHNGVKFIFTIYK
                     NLTLNDKGTGVLS"
     gene            complement(1569..3044)
                     /locus_tag="PKH42_03415"
     CDS             complement(1569..3044)
                     /locus_tag="PKH42_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19511.1"
                     /translation="MSEKDYNFDKQIIEQLSYVTPPEEEIRKTNPWSKPIGFITWGFI
                     LTTFKLNIIYLQYILPVIGVIHIFLGFRSLRKENKYFKIAWLLSIIKLSLHLIDLAWI
                     STPLNIMDYPNLIIGSVMLPFQISMYLVFRAALKETSKKAGRLMETDPLLWASLWTMA
                     AYLIALSPLSNSWLIFIPMVVSYIFIVRSLYHIGDEMDDTGYVLTNAPIKISNKIFGW
                     TYFLIAFAVVITCSVFSNHLKLQSQEYLHPETTETRQHLLDMGFPAEALRYLNNEDLM
                     MLSDAVNVESFSKLLMFDAKRIEHEERYGNYIQITHTYEPGKKNMDSTTVYIELPKNV
                     VYVMQYFKWKGGRPIWQDGILISGETAADDKQIISSGLFYSKKGTEYTADFPRLICDR
                     MTQHTMFGTYYPVLITGALSYPFGSEEQGGYVLYRYKVMTDSDIYDTYNIFSYVHLSS
                     PLHMPYTRTEDLILSGGYTFEDMLQQHQTNYESMALREKIK"
     gene            complement(3041..3568)
                     /locus_tag="PKH42_03420"
     CDS             complement(3041..3568)
                     /locus_tag="PKH42_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA19512.1"
                     /translation="MDEKIIELVKSSKDKGLTLMQEQYSGLMHYIVRGILQNKSDIEE
                     CISDICIKVWNSIESYSPEKGKFSTWLTVISRNTALNYMKSKYTEHDELKEETADKHS
                     VEDDVLRRERAEELKCAIRTLSPEEQHIFYRKYYYLQRTSQIAAELGMTERSVEGRLY
                     RLRKKLQKKLGGDFI"
     gene            complement(3737..4087)
                     /locus_tag="PKH42_03425"
     CDS             complement(3737..4087)
                     /locus_tag="PKH42_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOA19513.1"
                     /translation="MTNPSIYKMSVAKVYPFYVAKAEKKGRTKAEVDEIIRWLTGYSQ
                     EEFQEQLNKQTDFETFFLEAPLPNPARTLIKGVVCGVRVENVEEPIMREIRYLDKLID
                     ELAKGKAMDKILRK"
     gene            complement(4161..6806)
                     /gene="ppdK"
                     /locus_tag="PKH42_03430"
     CDS             complement(4161..6806)
                     /gene="ppdK"
                     /locus_tag="PKH42_03430"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOA19514.1"
                     /translation="MSKKWVYLFKEGSASQKSLLGGKGSNLAEMTNIGMPVPQGFIVT
                     TEACTEFYNQGEKLSEEIISQIRTNLSILEKQTEKVFGDTNNPLLVSVRSGAKFSMPG
                     MMDTILNLGLNEDTVEGIARKTGNTRFAYDSYRRFIQMFSDVVLGVPKPLFENALEAK
                     KEKNGYKFDTELTADDLKELVQEFKQIYKNEVGEDFPDNPEKQLLMAVEAVFKSWNNQ
                     RAITYRNLYNIPHDIGTAVNVQSMVYGNMGDTSGTGVAFTRNPSTGEDKVFGEFLINA
                     QGEDVVAGIRTPLTIDKLKDIMPEIYDEFINICEKLEQHYKDMQDIEFTIEEGKLYIL
                     QTRSGKRTADAALRAAVEMVDEGLISEEEAVLRVEPKSLDQLLHPKFDPVELKKSSPI
                     AKGLPASPGAASGKIYFTAEDAVEASLKGEDAILVRKETSPEDIEGMSKAKGILTSRG
                     GMTSHAAVVARGMGKCCVAGCESVSVDEKNKTMTVNKAVYHEGDYISLDGSTGSVYAG
                     IIKTVPANISGNFEKLMKWADEFRRLKVRTNADTPHDSEAAVKFGAQGIGLCRTEHMF
                     FEESRIFSVRKMIISDTVEQREKALAEIMPMQKNDFKGIFKAMGVRPVTIRLLDPPLH
                     EFLPTEEDDLIELAEAMNVSLQEVKAKVNSLHEFNPMLGHRGCRLAISYPEIAKMQTR
                     AIIEAAIEVSREENLEIIPEIMVPLVGKKEELLILRKLIIETAEETKKEFNSDLNYLV
                     GTMIEIPRACLTADEVAEYADFFSFGTNDLTQMTFGYSRDDAGKFLEDYKEARILEQD
                     PFETIDQEGVGILVDMAVKLGRKAKEHLHLGVCGEHGGDPASVEFFHRVGLDYVSCSP
                     FRVPVARLAAAQAALKDKMNVHIDK"
     gene            7047..8480
                     /gene="glnA"
                     /locus_tag="PKH42_03435"
     CDS             7047..8480
                     /gene="glnA"
                     /locus_tag="PKH42_03435"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOA19515.1"
                     /translation="MFSTVNEVQEYCVSNKIKMIDFKMIDINGRWRHLTIPAERLNED
                     TMKYGIGFDGSNYGYATVEKSDMIFIPDLTSAHIDPFVDIPTLSMIGNVFVLHPDGNK
                     PFNQYPRNVALRAEEYLRESKIADSMIIGPEFEFHVLDHISYECKPNKVAFEMDTSQA
                     EWNSGNNESRNLGYTVPLKSGYHITAPHDITYGLRSRMCLLLEDWGVEVKYHHHEVGG
                     PGQVEIEVELGELTEMADKTMIIKYVVKNSAIAENKTATFMPKPFFAEAGNGMHVHML
                     LMKDGKPVFYDENGYSGLSREAHWFIGGLLKHVRSLCGLTNPSTNSYRRLVPGYEAPV
                     TIGYATSNRSAVVRIPAYCKSPEAKRFEIRNPDGTCNPYYAYAAILMAGIDGIINKID
                     PHENGWGPYDFNLYNLSPEEQKKLSALPQSLDEALKALEEDHDYLLRGGVFPEELLNT
                     WIANKRMEMNEINKIPHPAEFAYYYDL"
     gene            8763..10664
                     /gene="dxs"
                     /locus_tag="PKH42_03440"
     CDS             8763..10664
                     /gene="dxs"
                     /locus_tag="PKH42_03440"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOA19516.1"
                     /translation="MKLLDSINSPEDIKHLNMDELNSLCRELREYILENVSKTGGHLA
                     SNLGVVELTVALHRVFNTPHDKIIWDVGHQTYAHKILTGRKNKMSSLRQLNGISGFPK
                     RNESIFDVFDTGHSSTSVSAAMGMARARDLNNEDYEIVAVIGDGALTGGMAFEAINDA
                     GRSNTKLLIILNDNEMSISKNVGGLSRYLSRIRTTKGYLSTKRDVEKILNKIPVGKQI
                     KSFLKRAKDGIKQIVVPGMLFEELGLTYMGPIDGHNIHKIIESLERAKNIEGPLLMHV
                     ITKKGKGYKFAEERPNEFHGVSAFNIETGEALKKSSQSYSDYFGMKMCEIAEKNENVV
                     AITAAMTDGTGLRDFAKKFPNRIYDAGIAEQHAVTMAAGLAVSGVVPVVALYSSFLQR
                     AYDQIIHDVALQNLHVVFAVDRAGLVGNDGETHQGVFDEGFLSLIPNMTVLSPADYRE
                     FGNMMEFAVSYNGPVAIRYPRGSMSEYIKNSEQSIVLGKGYIVEEGKDITIAAAGKMV
                     KTAIETREILKTKNIDAEVLNLRFIKPFDKELIINSVNKTKSLVIIDEATVFASYAVN
                     IYNILPKNLDVMVKTLPDEFIKQGSIEELLKENQLDADSIAAEIIKWKADNYDIDISN
                     NYTCLKQGR"
     gene            10789..11868
                     /locus_tag="PKH42_03445"
     CDS             10789..11868
                     /locus_tag="PKH42_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19517.1"
                     /translation="MNKVEIVVGDKKYSVRTDESPEYVRKIESVLNDQINIIANQNKR
                     FNEIDKLILSSFVVIDRYLKLSDEINEYKKDICEEILSLKEAKELSEKEKEEAVNKAA
                     DAIIEKERFKEKLMARDNDREYLNSQITKLQEKVSEQEQQLLKSEMLINELKIKNEEL
                     IEVNEELIKERENFTKEISFMNNTKASLNGRISKLQLKLNEKEQEIIKLEKTIRDLKS
                     AVDDKSQKIYNFSDEQQKMNLLVDSKEKDINSLINKINLLQNKLNEKDESIASKDKLI
                     NDLKSTEEVLKQKYESINDEKEKYLEELLMINSDKESLINSINQLQEKLNRKEAENFQ
                     NQLEINKLKKDNMELMQLLDEETAK"
     gene            11904..14075
                     /locus_tag="PKH42_03450"
     CDS             11904..14075
                     /locus_tag="PKH42_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOA19518.1"
                     /translation="MSVELLAPAGSIESFYAAVNSGADSVYLGGKFFNARYNSRNFDN
                     DEMKHIVKYAHNKGVKVYVTMNTVLKDNEITDALNYAIFLYENDVDALIVQDLGLFCL
                     VNKYLPDFPVNISTQASVYDEYGVKFFEEFNVKKVIMARELSLTELKETVKNTDADLE
                     VFIHGALCTCYSGQCYMSSFLGGRSGNRGKCAQPCRLNYSFYDKETKEEIESFKEVPL
                     LSLKDFIAGETIYELIEAGIKTFKIEGRMKGPAYTSSVVEYYRQIIDNYYSGKNIDST
                     HLKEKAMETFSRGYTNGYLKPSKDDEMFAKLSSGIKGENIDEIEEDVKDKAQEYSRFR
                     RNKISFDIELVIGEYALLKVFDGKNTVEVKSKEVCESSIKNNVTDEIIYEQLSKLGNT
                     IYELHDLKIVKDEGVFIRKSSLNQLRRDATEILYEKCSKFYDRRKVNTLTYNDIFSNI
                     NKESHSAKISLKINSIDELKYADDVKVKRLYVPFYLNIEEVKNTKVVEKYLWVPNIVS
                     KENYKILKENLNLYDEIFDGVCVNNVGSFYFFKKNSRLKLHCGPFFNIINTFSAELLK
                     EKGAHSFTFSVETNIRDMESIVKHTSLNSEIVAHEYVQLMVMKNCPMSVIKNCKNLQD
                     CEKCSFKSKYQLKDRKGIHFNIERQNKLTHIYNSVPLSIIGIANNFVEKNIDYFLVDT
                     KFLEDAEEIIDTLYCEIKGVKTHYILEENKFTRGHYLKNVL"
     gene            14290..16665
                     /locus_tag="PKH42_03455"
     CDS             14290..16665
                     /locus_tag="PKH42_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOA19519.1"
                     /translation="MKEKSKSVLEFDKIIEKVAGFAETKMGKEEVYKLDTSYNLEGVK
                     FKQEQTAQALSIIIEKGSPPLGGISDIKEYVKRGAVGGIISLRGLLNCADTLRAARLL
                     KNYVLLNNDDRNYDLLEDLCNSIYVNKDIEERIYSVIISEEEIADDASPELKRIRREI
                     QIKNNSIKNKINSIVSSSSMQKYLQEAIVTMRNDRYVIPVKKEYRSMVKGIIHDQSAT
                     GSTLFIEPMALVEMTNDIANLKIEEKKEIERILLELSMMIGDISEEMLNNQEILKELD
                     FIFAKGKYAISINGIEPKINDRGYIRIKKGRHPLISPELVVPIDAVLGDEYTTLIITG
                     PNTGGKTVSLKTLGLFTLMGMAGLHLPAEYGTEISVFSAVYADIGDEQSIEQSLSTFS
                     SHMTNIVKILKEVDEKSFVLFDELGAGTDPTEGAALAIAILDTLHERKILTAATTHYS
                     ELKLYALTTEGVINGSVEFDVETLSPTYKLLIGIPGKSNAFEISRKLGLQQEIIDKAK
                     KNIETEKIEFEDALKQIEENRIYIEEKKQEIDRLESESKKKHSDFLMKERKSLEKSEK
                     IIDEANYEARKIVENAKREAAEIIKELRKLNLEMDKDKNRRVNELRQALNEKTKELTE
                     NIYSDIIEEEDTIDTDTPLKIGDSVTVRSLNQKGFIISDVDDSQTVMVQIGLIKTKVK
                     KSDLIKIKSDEEVKQKTNASRKVRLRTSSINPTIDIRGYNLDDALLELDKYLDDAFMS
                     NLNEVQVIHGKGTGVLREGVRQFLKNHKHVKESRLGTFNEGGDGVTIVKFK"
     gene            16729..17811
                     /locus_tag="PKH42_03460"
     CDS             16729..17811
                     /locus_tag="PKH42_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19520.1"
                     /translation="MNVRDKYSNKAVIKYTGAFIAWVIGSGFATGQEALQFFSSYGYL
                     SYGVLLVNLIGFLFLGQVLLVTGFEHKTESSFNHFKYFCGEKIGTIYSWLIPITLILI
                     MPVLISGAGATLSEYYGVNRYVGSFLMSVLVLSVYLIGFEHLIKIVSAIGPAIIIFSL
                     LVGAITLANDYGNFSDVPKYQFKLNELQTAPHWTISAILYLSLNFLSGSVYFTELGKS
                     AYNRNDVKYGAFFGALAIILNIAIMNTAILLNAENISTLAIPVLYLAKKISYMLGAIF
                     SIILILGMFSSCSAMMWSICNRFTRGGKRGNRIFAVFISILTFVLGLFSFSELIGTFY
                     PLVGYVGLFFIGSVIYKGIKYNIIKN"
     gene            17822..18043
                     /locus_tag="PKH42_03465"
     CDS             17822..18043
                     /locus_tag="PKH42_03465"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOA19521.1"
                     /translation="MERIIKDVNCPKCGQVMEEGYIHSRKQILWSEDDKPRLFDFYDE
                     NLVTMPIRRANKVPAWRCKECKIAIFRYE"
     gene            18165..18944
                     /locus_tag="PKH42_03470"
     CDS             18165..18944
                     /locus_tag="PKH42_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOA19522.1"
                     /translation="MRQYVVDAFAEKVFEGNPAAVCILDEWLPDEVMLNIARENNLAD
                     TAFVVKDGTNFKLRWVTPGGEIDLCGHATLATGYVISRFIAPELSEVVFQTKSSGILK
                     VLKKGELYEMDFPAYKLKKVEVTNAMADAIGVKPIEAYMGRDLLCVLESEDEVRNLNP
                     NLEKVKLLDGLLFHVTAKGNEFDCVSRSFVPKLSGTEDPVCGSGHCHIIPYWAERLGK
                     DNLIAYQASQRGGTLYCKYQGDRVIMGGKVALFSTADINIE"
     gene            19015..20094
                     /locus_tag="PKH42_03475"
     CDS             19015..20094
                     /locus_tag="PKH42_03475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19523.1"
                     /translation="MEREIVRLDMDRDFEAFEDLLKISFGEATNSKKEMYEWLFENNP
                     YNKSGNMMYLLKEGDKVIGCDGLLPNELYVNGKTLLTAHSVKSMTHPDYKRQGIFRKM
                     TENSCERGKQDGVDVIIGLANDQSYPAYQKFGWPTLFEKEVYVKPILINNILKRRIKI
                     GFLSEAANSVYTVFMKNKLKNQMDKEIKFEILDRVPEEIQKCWDRYKSKYNVLLVRDY
                     KYLNYRYNERPDINYVTILARHKGEIIGFAILHNAVANGSNMTSAVEFFTDPDNERYI
                     KALANGIAKYCYDKGLEYAVIGTGHYGEYRKVLLNNGFMVTRKPPKNNMMIAHILSDK
                     VTLEEITGHEKWHITQGDGETELDL"
     gene            20384..21253
                     /locus_tag="PKH42_03480"
     CDS             20384..21253
                     /locus_tag="PKH42_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOA19524.1"
                     /translation="MIKLIVDSTCDLNCEILDNHDVEVIPLSITIDDKTYLDGVDITT
                     DRVYEYMKDGKVPKTSQVSFESVSQAVEKCISKESDFIYLAFSSKLSGTYSFAKKIID
                     MYKEKYPERKMAIIDSKGGAGGTGLIAIQVLKMIERNLPFEYILNQAQFMVQHIVYRF
                     TISDLEWLSKGGRISKSAGYVGNVLNIKPYLVLDDGLIKVSKMIRGSRKTIQTLIQDI
                     EDGTDKFKNQIIGISHADDLDTALMVEQKIKDSIKGCKTTIFKIGAVLGVHLGIGGVG
                     VFFFDEKPEFYEL"
     gene            21406..23112
                     /gene="argS"
                     /locus_tag="PKH42_03485"
     CDS             21406..23112
                     /gene="argS"
                     /locus_tag="PKH42_03485"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOA19525.1"
                     /translation="MDFKEKVAEIFTSIETELSMDDALQLIEIPPSSDMGDYAVPCFK
                     FAKKYRKSPAAIANEIVEKIGAVEEFEKIESAGPYVNFFVNKSQFAKKILKQAFEEKE
                     NYGRTNVGEGKTVIVEFSSPNIAKPFHIGHIRSTLIGNAINNIYKYLGYNTVAINHLG
                     DYGTQFGMLISAYKKWATPEIIEAIEKDPIPELLKLYVRYNQEIETHPEYQEEARYWF
                     NQLEKGNQEAYQLWAWFREVSLKEFNRVYDMFGIKFDSYAGESFYSDKMPAIIDELED
                     KGLLKDSEGAKIVELSEFGLTDALIQKSDGATLYVTRDVAAAKYRKETYNFYKNIYVV
                     GAQQKLHFDQWRKIIDLMGYDWAYDCVHVMFGTVSLEGGAIATRKGRVVFLEDVLNKA
                     IEKTTEIINERNPGLEDREKVAKQVGIGAVIFQELFNNRIKDYVFSWDKTLSFEGETG
                     PYVQYTFARTCSLLRKADIDINDDVDYSVLTDSESFNVIKKLEGFKEAVLNAHDKYEP
                     FMITRYIVGLAQEFNRFYHECQIITDDESVKKARLLLTLTVNTVLKQGMKLLGMEAPE
                     KM"
BASE COUNT         7978 a         3706 c         4552 g         6898 t
ORIGIN      
        1 aatgcaaaca atgaagcaaa agctttggct ataatatata atgcgtggga ggaattatga
       61 gtactgcaga taaaattttt aaagagatgt gtaaagatat attgactaag ggagtatggg
      121 atactgatta cgatgtaaga ccaagatggg aggacggcac gcctgctcac actattaaga
      181 agtttggcat agtaaacaga tacaatttac aggaaagttt tcctattcta acactgagaa
      241 aaacaaattt cagagctgca gttgatgaac ttttgtggat atggcagaaa aaatcaaata
      301 acgtaaatga tttaaacagc cgtatttggg attcctgggc agacgaaaca ggctccattg
      361 gcaaggctta cggttaccaa ctcggaataa aacataaata caaggaaggc gaattcgacc
      421 aggtagacag ggttttatat gatttaaaaa ataatccggc aagcagaaga attttaacaa
      481 atatttataa tttccaagac ctgcatgaaa tgcacttata tccatgcgca tacagcatga
      541 ctttcaatgt ttccggaaac aagttaaatg ccatattaaa tcagcgctct caggatgtct
      601 tggcagccaa caactggaat gtggtgcaat attcaatatt ggtacatatg atggctcagg
      661 taagcggact ggttgcagga gaattagttc atgtaattgc cgatgctcat atttatgaca
      721 agcatgttcc cataatagaa gagcttattg aaatggaaga gcatgagccg ccgacattgg
      781 aaatagataa tatagatgat ttttataaat ttactgtcga tagttttagg ttgattaatt
      841 accaatacaa tccttttaaa gtaaaggtac cgatagcggt ataaaggaga aaccatgaaa
      901 ttaatagttg cggtagcaag agattgggga ataggaaata agggagagct cttgttcaat
      961 gtgcctgaag atatggaatt ttttaaaaaa actacattga acaaggtcgt cgttatgggc
     1021 agaaaaacac ttctttctct tcccggcggg acccccttaa aaaacagaac aaatatagtt
     1081 atgacaagtg atgtgaattt taaaaaggaa ggttgcatca tagttaattc aaaagaagct
     1141 ttgtttgaag agttaaaaaa atacgagagc gacgatgtgt ttttaatagg tggaggtata
     1201 ttgtacaagg agctttatcc ctattgcagt gaagcttata ttaccaaatt tgacgccata
     1261 ttggaagcgg atacatattt gcataatttc gatgaagaca aggattggct tctcacttat
     1321 gcatcagaaa tacatgaaca caacggagta aaatttattt tcaccatata caaaaactta
     1381 accttaaatg acaaggggac gggggtactg tcataatttg ataatactcc gtccccttga
     1441 cccggagggg acggttctct ccggacaatt aaactaaatg acatcaatga aaccatcctc
     1501 ggtttaaaat aagtgctgaa atatctttct atcaacccac cttcataaga actaaaccat
     1561 tcccaaaact atttgatttt ttctcttaat gccatagatt cataatttgt ttgatgttgc
     1621 tgtaacatat cctcaaaggt ataaccacca cttaaaatca agtcctctgt ccttgtgtat
     1681 ggcatgtgta atgggcttga aaggtgtacg taactaaaga tattataagt atcataaata
     1741 tcactgtcag tcatgacctt atagcgataa agcacatagc cgccctgttc ttcagagcca
     1801 aagggataac ttaaagcacc cgttatcagt acggggtagt aggtgccaaa cattgtgtgt
     1861 tgtgtcattc tgtcacatat aaggcgggga aaatccgcag tatactctgt gccttttttg
     1921 ctgtaaaaga gaccgctgct gattatctgt ttatcatccg ccgctgtttc tcccgatatc
     1981 agaattccat cctgccagat tggccgtcca cccttccatt tgaagtattg cattacatat
     2041 accacatttt tcggtaactc gatgtagacc gtagtagaat ccatattttt tttacccggt
     2101 tcataagtat gagttatttg aatgtagttt ccatatcttt cctcatgctc aattctcttt
     2161 gcatcaaaca ttagcaactt actgaatgat tccacattca cagcatcact tagcatcata
     2221 aggtcttcat tgttgagata tcgcagggct tcagccggaa atcccatgtc aaggagatgt
     2281 tgccttgttt cagtagtttc tggatgcaaa tattcttgtg attgtaattt caggtgattg
     2341 gaaaaaacac tgcatgttat cacaactgca aatgcaataa gaaaataagt ccatccgaat
     2401 attttgttgc tgattttgat aggtgcattt gtcagaacat atccggtatc gtccatttca
     2461 tctcctatat ggtataatga gcgcacaata aaaatatagg acactaccat aggtataaaa
     2521 atcagccaac tattggaaag tggtgataga gcaataagat atgctgccat tgtccataaa
     2581 gacgcccaaa gtaatgggtc agtttccatt aacctgcctg cttttttaga agtctctttc
     2641 aaagctgcac ggaaaacgag atacatagat atttggaatg gaagcattac agagcctata
     2701 atgagattgg gatagtccat aatgtttaag ggcgttgaaa tccaagccag gtctatcagg
     2761 tgtaatgata gctttattat ggaaagtaac catgcgattt taaaatattt attttccttt
     2821 ctcaggctgc gaaagccaag aaaaatatga ataacaccta taacaggcaa aatatattgt
     2881 aggtatataa tattcaattt aaatgttgtc aggataaaac cccaggtgat aaagccaatg
     2941 ggtttgctcc agggatttgt ctttcggatt tcctcttccg gaggggtcac gtagctgagc
     3001 tgctcaatta tctgtttatc aaagttataa tctttttcgc tcatatgaaa tcacctccta
     3061 atttcttctg cagctttttt cgcagtcggt atagtcttcc ttcgacagag cgttctgtca
     3121 ttccaagttc tgcagcaatt tgagatgtgc gctgcaggta ataatattta cggtagaaaa
     3181 tatgctgttc ttccggagaa agtgtgcgta tagcacattt taattcctcc gcccgttccc
     3241 ggcgaaggac atcatcttct acagagtgtt tatccgctgt ctcctctttt agctcgtcat
     3301 gttcagtata cttgcttttc atataattaa gcgctgtatt tcgtgatatt acggtaagcc
     3361 atgtggagaa tttgcccttt tccggagaat agctctcgat ggaattccaa actttaatgc
     3421 agatatcgct tatacattct tctatgtcgc tcttattttg caaaatccct cttactatat
     3481 aatgcatgag ccctgaatac tgttcttgca tgagcgttaa gcctttgtcc tttgatgatt
     3541 tcacaagctc aataatcttt tcatccacat gcccacctcc tccatcttcc attatattat
     3601 acaagtaaat ttgtagaatc ccacgaaata ttataaagtt cctgaaaatc gcaagatata
     3661 aatatggtac tgtttcttat ttttttaaga aacagtacct cagattattt acaaacctaa
     3721 tcttcctatt ttgaatttat ttgcgtaaaa tcttatccat tgcttttcct tttgccaatt
     3781 catcaatcag tttatccaaa taacggattt cacgcatgat tggttcttca acgttctcta
     3841 ctcgaacacc gcaaactaca cctttaatca gggttcttgc aggatttggc aggggagctt
     3901 ccaaaaagaa agtttcaaag tctgtttgtt tattaagctg ctcttgaaat tcttcttggc
     3961 tgtatcctgt taaccaacgg ataatttcat cgacttctgc ttttgtgcgt ccttttttct
     4021 ccgccttggc aacataaaag ggatagacct ttgccacgct cattttatag atagagggat
     4081 tggtcatgta agcctcctgt attaatatga tcgggacggg gatgtcattt atgacactac
     4141 ccccgtcccc ttgtcatctt ttatttatcg atatgaacat tcattttatc ttttaatgca
     4201 gcttgtgcgg cagcaagtct tgcaacagga actctgaatg gtgagcatga tacatagtca
     4261 agccctactc tgtggaaaaa ttcaactgag gcagggtctc cgccatgctc cccgcatact
     4321 cccaggtgca aatgttcctt tgcttttctt cctaatttta cagccatgtc tactagtatg
     4381 cctacacctt cctggtcgat agtttcaaag gggtcttgct cgaggattct tgcttccttg
     4441 tagtcctcta ggaatttgcc tgcatcatcc cttgaataac caaatgtcat ctgtgtgagg
     4501 tcatttgttc caaaggagaa gaaatcggca tattctgcta cttcgtcagc tgttaagcat
     4561 gcacgcggta tttcaatcat tgtgcctaca agatagttca aatcggaatt gaattccttt
     4621 ttagtttctt cagctgtttc gattattaat tttcttagta tcaatagttc ttcctttttg
     4681 cctacaagcg gaaccattat ttccggtata atttctaaat tttcttccct tgaaacttca
     4741 attgcagctt caattattgc tcttgtctgc atttttgcta tttcaggata tgaaattgcc
     4801 agacggcagc ctctgtgacc aagcatcggg ttgaattcgt ggagactgtt gactttggct
     4861 tttacttcct gtaaagaaac attcatagct tcagccaatt ctattaaatc atcttcctct
     4921 gtaggtaaaa actcatgaag cggagggtca agaagcctga ttgtaacagg acgaacgccc
     4981 attgctttaa atattccttt aaagtcgttt ttctgcatag gcataatttc tgccaatgct
     5041 ttttctcttt gctcaactgt atcagaaata atcatttttc taacggagaa tatacggctt
     5101 tcctcgaaga acatgtgctc agtcctgcaa agtcctatac cttgtgctcc gaatttaacg
     5161 gctgcttctg aatcatgggg tgtatccgca tttgttcgaa ctttaagtct tctgaattca
     5221 tctgcccatt tcattaattt ttcaaaattt cctgatatgt tggcaggtac tgtttttatt
     5281 attcccgcat atacgcttcc tgttgagccg tcgagtgaaa tataatcgcc ttcatggtaa
     5341 actgctttgt ttacagtcat tgttttattc ttttcatcta ctgataccga ttcacagccg
     5401 gcaacacagc atttacccat acctcttgca acaactgcag catgagaggt cattcctccc
     5461 cttgaagtca gtatgccttt agctttgctc attccttcaa tatcttcggg agatgtttct
     5521 tttcttacaa gaattgcatc ttcacctttt aatgaagctt caaccgcatc ttcggctgta
     5581 aagtatattt tcccggaggc tgctcccggt gaagccggca gcccttttgc aataggtgat
     5641 gattttttaa gctctacagg gtcaaactta ggatgcaaaa gctggtctaa tgatttcggt
     5701 tcaactctta aaactgcttc ttcttcagat atcaatcctt catcaaccat ctcaaccgca
     5761 gctcttaatg ctgcatcagc tgttcttttg cctgaacgtg tttgaagtat gtagagtttg
     5821 ccttcctcta ttgtaaactc tatatcctgc atgtctttgt aatgctgctc gagtttttcg
     5881 caaatattta tgaattcatc atatatttca ggcataatat cctttagctt atcaattgtt
     5941 agaggtgtac gtattccggc aacaacatct tcgccttggg catttatcag gaattcaccg
     6001 aatactttgt cctctccggt ggacgggttt cttgtgaatg caactcccgt tcctgatgtg
     6061 tctcccatgt tgccgtatac cattgactga acatttactg ctgttcctat atcgtgaggt
     6121 atattgtata aattcctgta tgtaatagct ctttggttat tccatgattt aaatactgct
     6181 tctactgcca ttaaaagttg tttttccgga ttatcgggaa aatcttcacc tacttcattc
     6241 ttgtaaattt gtttgaattc ctgtacaagt tcttttaaat catctgcagt caattcagta
     6301 tcaaatttgt aaccgttttt ttctttttta gcttctagag cattttcaaa caaaggttta
     6361 ggaactccta atactacatc gctgaacatt tgaatgaatc ttctgtaact gtcgtatgca
     6421 aagcgtgtat ttcctgtttt tcttgcaata ccttcaacag tatcttcatt taaaccaagg
     6481 tttaaaattg tgtccatcat tcccggcatt gaaaatttag ctcccgagcg gacagatact
     6541 aaaagagggt tattagtatc accaaatact ttttctgtct gtttttccag aatagataaa
     6601 ttagtcctga tttggctgat aatttcctca gataattttt ctccttgatt gtaaaattcc
     6661 gtgcaagcct ccgttgtaac tataaatcct tgaggtacgg gcattcctat attagtcatt
     6721 tctgctaaat ttgagccctt tcctcccaat aagctttttt gagaagcact tccttctttg
     6781 aataagtaaa cccatttttt actcatatga acctccttct atattcctat tattattgca
     6841 aactcattaa ttgagtatta ttcctaaaat aaattatata gaccaataat attataaatt
     6901 taataaaaag tacagtatta tttcaaaaaa tataaaataa tataccggac tgacatataa
     6961 cggcaaggtt aaataaggct tgttttaatt tttatatgtt gtataatatt accataagct
     7021 acctaaaaaa ttttttggag gtttttatgt tttcaacagt aaatgaagtg caagagtatt
     7081 gtgtcagtaa caaaattaaa atgattgact ttaaaatgat tgacataaac ggacgctggc
     7141 gtcatttaac tattcccgcc gaaagactta acgaagatac aatgaagtat ggtatcggtt
     7201 ttgacgggtc aaattacggt tatgctaccg ttgagaaaag cgatatgata tttattcctg
     7261 atctcaccag cgcacatatc gacccttttg ttgatatacc tacactttct atgattggaa
     7321 atgtatttgt tttacatcct gacgggaata aaccctttaa tcaatacccc aggaatgttg
     7381 cattaagagc cgaagaatat ttacgagaaa gtaaaattgc tgattccatg attattggtc
     7441 ccgaatttga atttcatgtt ctggatcata ttagttatga atgtaaacca aacaaggtag
     7501 cttttgagat ggatacatca caagctgagt ggaacagcgg taataatgaa agcagaaatt
     7561 taggttatac cgtaccttta aaaagcggtt atcatataac tgcgcctcat gatataactt
     7621 acggactaag gagcaggatg tgtcttcttt tggaggattg gggcgtagaa gttaaatatc
     7681 accaccatga agtaggcggc cccggacagg ttgaaattga ggttgagctt ggagaattaa
     7741 ctgaaatggc cgacaaaacg atgataataa aatatgttgt taagaattcc gccatagcag
     7801 aaaacaaaac tgcaactttt atgcctaaac cgttttttgc ggaagccggg aacggcatgc
     7861 acgtacatat gctcttgatg aaggatggaa aacctgtttt ctatgatgag aacggatatt
     7921 ccggactcag ccgggaggct cattggttta taggaggttt attgaaacat gtcaggtccc
     7981 tttgcggttt gacaaatcct tccacaaatt catacagacg ccttgtgccg ggatatgagg
     8041 ctccggttac gataggttat gcaacttcaa acagaagtgc agttgtacga atacctgcgt
     8101 attgcaaatc gcctgaagca aaacgttttg aaataagaaa tccggatgga acatgcaatc
     8161 cttattatgc ttatgcagct atacttatgg caggtataga cggtataatc aataaaatag
     8221 accctcatga aaacggctgg ggtccatatg attttaatct atacaatctt tcacctgagg
     8281 agcagaaaaa actttcagcc cttcctcaat cattggacga agctcttaaa gctctggaag
     8341 aagaccatga ttatttgctt cgcgggggag tattccctga agaattactg aatacatgga
     8401 ttgcaaataa aagaatggaa atgaacgaaa tcaataaaat acctcaccct gctgaatttg
     8461 cttattatta tgatttgtag ttgttctatg aattagcgcc tttccgaatg cacattatta
     8521 cctccaaagt agataatgta attaattatt ttacaaaatc tatcaggagg taaatttttt
     8581 gtatacagaa atcagtattg tcttagtata tgtgggattg gattaattga atatattatt
     8641 atagctttga cataataatt caaacataat ataattataa aaaacaaaga gcttgttgat
     8701 agcgttcatt gcatggtaat gcaaatgaat aatgcatttt aaatacgtat agtgaggtta
     8761 aaatgaaatt actggatagt ataaattcgc ctgaagatat caagcatttg aatatggacg
     8821 aattaaattc tctttgcagg gaattaagag aatatatttt agaaaacgtt tcaaaaacag
     8881 gaggacatct tgcatctaac ttgggagtgg tagagctcac tgtagccctg catcgagttt
     8941 tcaatactcc tcatgataaa ataatatggg atgttggtca tcaaacgtat gcccataaaa
     9001 ttcttactgg aagaaaaaat aaaatgtcat ccttgcggca gcttaatggt ataagcgggt
     9061 ttcccaaaag aaatgaaagt atttttgatg tgtttgatac gggacacagc agcacctctg
     9121 tttctgcagc aatggggatg gcaagagcaa gagacttaaa taatgaggat tatgaaattg
     9181 ttgcagtaat aggagacgga gcccttaccg gaggaatggc ctttgaagca ataaacgacg
     9241 cgggaaggag caacacaaaa ttattgataa ttttaaacga caatgaaatg tccatatcca
     9301 aaaacgtagg aggcttgtcc cgctatttaa gcagaataag aactaccaaa ggatatttgt
     9361 ccactaaaag ggatgtcgaa aaaatattaa ataaaattcc ggtcggtaaa cagattaaat
     9421 catttttaaa aagagcaaaa gacggaatta agcaaatagt tgttccgggg atgctttttg
     9481 aagaattggg gcttacctat atgggaccta tcgacggtca caatatacat aaaattattg
     9541 aatccctgga aagggcaaaa aatattgaag gccccttatt aatgcatgta attacgaaaa
     9601 aaggcaaggg ctataagttt gctgaagaaa gaccgaatga attccatggt gtttctgctt
     9661 ttaatataga aacaggagaa gctctaaaaa aatcctctca atcttattcc gattattttg
     9721 gaatgaaaat gtgtgaaatt gcggaaaaaa atgaaaatgt ggtcgcaata acagcagcta
     9781 tgactgatgg tacggggctt agggattttg caaaaaaatt ccccaataga atttatgatg
     9841 caggcattgc agagcaacat gcggtgacaa tggctgcagg gcttgccgta agcggggttg
     9901 tcccggttgt tgcactttat tcgtcatttc tacaaagagc ttatgaccaa atcatccatg
     9961 atgttgcctt acaaaacctt catgttgttt ttgctgttga cagggcaggt ttggtgggaa
    10021 atgacggcga aacgcatcaa ggtgtgtttg atgaaggatt tttgagttta attcccaata
    10081 tgacagtatt atcacccgct gattacaggg aattcggaaa tatgatggaa tttgccgttt
    10141 catataacgg acctgtagca ataagatatc cgaggggcag catgagtgaa tatatcaaaa
    10201 acagcgagca aagtatagtt ctcggcaagg gatacatagt cgaggaaggg aaggacataa
    10261 caatagctgc agccggtaaa atggtaaaga cggcaataga aacaagggaa attttaaaaa
    10321 caaaaaatat agatgcagag gttcttaatt taagatttat aaaaccattt gacaaagagc
    10381 ttataataaa ctccgtaaat aaaacgaaat cattggtaat aatcgacgaa gcaactgttt
    10441 ttgcttccta tgcagttaat atttacaata tacttcctaa aaatcttgat gtaatggtta
    10501 aaactttgcc cgatgaattt attaaacaag gctcgattga agagctctta aaagaaaatc
    10561 aattagatgc tgattcgata gctgctgaaa taattaagtg gaaggcagat aattatgata
    10621 ttgatataag caataattat acttgcttaa aacaaggacg ttagagcgga agtagaatta
    10681 ttaaattagt gaaaatgtat tatttactat tgaaatttta agggttttca tttatattat
    10741 agttacggat atattttact cacgataaaa aataaagggg atttgaatat gaataaagta
    10801 gaaattgttg ttggagataa aaaatatagt gtaagaactg atgaaagtcc cgaatatgta
    10861 agaaaaatag aatctgtttt gaacgatcaa attaatataa tagctaatca gaataagcgt
    10921 tttaatgaaa tagacaaatt aattctgtca tcttttgtgg ttattgacag gtatttaaaa
    10981 ctttccgatg aaattaacga atacaaaaaa gatatatgtg aagaaattct atctttaaag
    11041 gaagcaaaag aattatccga aaaggaaaag gaagaagctg taaataaggc agcggatgcc
    11101 attattgaaa aagaaagatt caaagaaaag ctaatggcaa gagacaatga ccgtgaatat
    11161 ttaaattccc aaataacaaa gcttcaggaa aaagtcagtg agcaggaaca gcagctcttg
    11221 aagagcgaaa tgctcataaa tgaattaaaa ataaagaacg aagagttgat tgaagtaaat
    11281 gaagagctaa tcaaagaaag agaaaacttc acaaaagaaa taagctttat gaacaataca
    11341 aaagcaagct taaacgggag aatttcaaag ctgcagttaa aacttaacga gaaagaacag
    11401 gaaataatca aacttgaaaa gactatcagg gatttaaaaa gcgcagtaga tgataaatca
    11461 caaaagatat ataatttcag cgatgagcag caaaaaatga acttattggt tgattcgaaa
    11521 gaaaaagata tcaattcatt aataaataaa atcaatttgc tgcaaaataa gcttaatgaa
    11581 aaagatgaat caattgcttc caaggataaa ttaataaatg atttaaagag cactgaagaa
    11641 gtattaaaac aaaaatatga atcgattaat gatgaaaaag aaaaatattt agaagagctt
    11701 ttaatgataa acagcgataa agaaagcctt atcaacagca taaatcagct gcaggaaaaa
    11761 ttaaaccgca aggaagcgga aaacttccag aaccagttgg aaattaataa gctgaaaaag
    11821 gataatatgg agcttatgca gcttttagac gaagaaacag caaaatagaa cgcgtcggcg
    11881 ttctttttta aacaggagaa gaaatgagcg tagaattatt agcgccggca ggcagtattg
    11941 aatcatttta tgcagctgta aacagcggag cagattcagt atatttaggc ggaaagtttt
    12001 ttaatgcaag atataattcc cggaattttg ataatgatga aatgaagcac attgtaaaat
    12061 atgctcacaa caagggtgta aaagtatatg ttacaatgaa tactgtttta aaggataatg
    12121 aaattacgga tgcattgaac tatgcaatat ttttgtatga aaatgatgta gatgccttaa
    12181 tagttcaaga tttaggtttg ttctgcctgg taaataaata tttacccgat tttcccgtaa
    12241 acattagcac acaggcaagt gtatacgatg aatacggtgt taaatttttt gaagaattta
    12301 atgtcaaaaa agttattatg gcaagagaac tgtccttaac agagctaaaa gagacagtta
    12361 aaaatacaga tgccgatttg gaagtattca ttcacggtgc cctttgcaca tgctattcgg
    12421 gacaatgcta catgtcaagc tttttgggtg gaagaagcgg aaaccgaggg aaatgtgcac
    12481 agccgtgcag attaaactac agcttttatg acaaagaaac aaaggaagaa attgaaagtt
    12541 ttaaagaggt gcctctgctt agtttaaaag actttatagc aggggaaaca atttatgagc
    12601 ttattgaagc aggaattaaa accttcaaaa tagaagggcg catgaaaggt cctgcatata
    12661 cttcatcagt tgtggaatat tacagacaaa taatagataa ttattacagc ggcaaaaata
    12721 ttgacagtac tcatctaaaa gaaaaggcta tggaaacatt cagcagaggt tatacaaacg
    12781 gatatttaaa gccttcaaaa gacgatgaaa tgtttgcgaa attaagttcc ggcataaagg
    12841 gagaaaatat cgatgaaata gaagaagacg taaaggataa ggcacaggaa tattcaaggt
    12901 tcaggagaaa taaaataagc tttgacatag agcttgtgat aggcgaatat gccctgttaa
    12961 aagtttttga cgggaaaaac acggttgaag taaaaagtaa agaggtctgc gagagcagca
    13021 ttaaaaacaa tgtaacagat gaaataattt atgagcagct ctcaaagtta ggtaatacta
    13081 tatatgaact gcatgattta aagattgtaa aagatgaagg tgtttttatc agaaaaagtt
    13141 ctttaaatca attaaggcgt gatgcgacgg aaattcttta cgaaaaatgc agcaaatttt
    13201 atgacagaag aaaagttaat actcttacat acaatgatat attttcaaat atcaataaag
    13261 aaagtcactc tgcaaaaata agccttaaaa taaacagcat tgatgaatta aagtatgctg
    13321 acgatgttaa ggttaagcgc ttatatgtgc ctttttattt aaatattgaa gaagtaaaaa
    13381 atactaaagt tgtagaaaaa tatttatggg ttccaaatat agtatctaaa gaaaattata
    13441 aaattttaaa agaaaatttg aacttgtatg atgaaatatt tgacggtgta tgtgtaaaca
    13501 atgttggaag tttttatttc tttaagaaaa atagcaggct gaagcttcac tgcggaccgt
    13561 tttttaatat tatcaatact ttttcagcag aacttttaaa ggaaaaggga gctcacagct
    13621 ttacattttc tgtagaaaca aatatcaggg acatggaaag tattgtgaaa cacacatcat
    13681 taaactcgga aattgtcgcc catgaatatg tacagcttat ggttatgaaa aattgtccca
    13741 tgtcggtgat aaagaattgt aaaaacctgc aggactgtga aaaatgcagc ttcaaaagca
    13801 aatatcaatt aaaagacaga aaaggaattc attttaatat cgaaagacaa aataaactta
    13861 ctcatatata taacagcgtg cccctgtcga ttataggaat tgcaaataat ttcgttgaaa
    13921 aaaatattga ttatttttta gtcgatacaa aatttctaga ggatgcggaa gaaataatag
    13981 atacattgta ctgcgaaata aagggagtta aaactcatta tattttggag gaaaacaaat
    14041 ttacgagagg gcactatttg aagaatgtgt tgtaacacac ttgtcatcct gagcaaagcg
    14101 aaggatctca tgagattctt cgagctaaag ctctcagaat tatcacgtgt ttatcacacc
    14161 acgataaacg aatattctct gtgattgtca tcctgagcaa agcgaaggat ctcatgagat
    14221 tcttcgagat aaagctctca gaatgacaaa atgctaatca tagcaatgac aaacaggaaa
    14281 ggaaaaacga tgaaagaaaa atctaaatca gttttggaat ttgataaaat aattgaaaag
    14341 gttgccggat ttgctgaaac caagatggga aaagaagagg tgtacaaatt agatacatct
    14401 tacaacctgg aaggtgtaaa attcaagcag gagcaaacag cacaggcact ttccataatc
    14461 attgaaaaag gttcacctcc cttgggaggt atttcagata taaaggaata cgtgaaaaga
    14521 ggagccgtag ggggtattat atctctaaga gggctattga actgtgccga taccctaaga
    14581 gctgcaaggc ttctaaaaaa ttatgtcctt ctgaataatg acgacagaaa ttatgatttg
    14641 ctggaagact tgtgtaacag tatatatgta aacaaggata ttgaagaaag aatatattcg
    14701 gttattatca gcgaggaaga aattgccgat gatgctagcc cggagcttaa gcgcataaga
    14761 agagaaattc aaataaaaaa caattcaata aagaataaaa ttaattcaat cgtaagctca
    14821 tcttccatgc agaaatatct gcaggaagca atagtaacca tgcgcaatga cagatatgta
    14881 atccccgtga agaaagaata cagaagtatg gtcaaaggaa taattcacga ccagtctgca
    14941 acaggctcca cgctttttat agagcctatg gcccttgtgg aaatgaccaa tgatattgcc
    15001 aatttaaaaa tcgaagaaaa aaaggaaatc gagcgtatac tattagagct tagcatgatg
    15061 ataggtgata taagtgaaga aatgctaaac aatcaagaaa tactaaaaga gcttgacttt
    15121 atttttgcaa agggcaaata cgccataagc ataaacggaa ttgagcccaa gataaatgac
    15181 agaggataca taaggataaa aaagggaaga catcctctca tttcacctga attggtggta
    15241 cctatcgatg cagtgcttgg tgacgaatat acaaccctaa taataacggg acctaatacc
    15301 ggaggaaaaa ccgtttcttt aaaaacctta gggcttttca ctcttatggg aatggcaggg
    15361 cttcatctcc ctgccgaata cggcaccgaa atatctgttt tcagcgcagt ctatgccgat
    15421 ataggcgatg agcagagcat cgagcaaagc ttgagcacat tttcctctca tatgacaaat
    15481 atagtgaaaa tattaaaaga ggttgatgaa aagtcattcg ttcttttcga cgagctcggt
    15541 gcgggaacgg accctactga aggtgctgcg ttggcaattg caatacttga cactctccat
    15601 gaaagaaaga ttctgactgc tgcaaccaca cattactcag agcttaaatt atacgctctt
    15661 acaaccgaag gagtaataaa cggaagtgtt gaatttgatg ttgaaacatt aagtccgacc
    15721 tacaagcttt taatcggtat acccggtaaa tccaatgcct ttgaaatatc aaggaaacta
    15781 gggcttcagc aagaaattat cgataaggcc aaaaagaaca tagaaaccga gaaaattgaa
    15841 tttgaagatg ccttaaaaca aattgaagaa aaccgaattt atatcgaaga gaagaaacaa
    15901 gaaattgaca gactcgaatc agaaagcaaa aaaaagcaca gcgacttttt gatgaaggaa
    15961 agaaaatcac ttgaaaaaag tgaaaaaatt atagatgaag ctaactatga agcaagaaaa
    16021 atagtcgaga atgccaaaag ggaagctgct gaaattatca aggaattaag aaaattaaac
    16081 cttgaaatgg ataaggataa aaaccgccgt gtaaacgaat taagacaggc actaaatgag
    16141 aaaacaaagg agcttacaga aaatatttat tcggatatta ttgaggaaga agatacaatt
    16201 gacacagaca cacctttaaa aatcggtgac tccgtaactg taagaagtct aaatcaaaag
    16261 ggattcatta tttctgatgt ggatgattca cagactgtaa tggtccaaat aggactaatt
    16321 aaaacaaagg ttaaaaaatc cgacctaata aaaataaaat ctgatgaaga agtaaaacaa
    16381 aaaaccaacg catcccgtaa ggtcagacta agaacttcgt caatcaatcc tacaattgat
    16441 atcagaggat acaatcttga tgatgctttg ttggagctgg ataaatattt ggatgatgcc
    16501 tttatgtcaa atttaaacga agtccaggta attcacggta aaggtaccgg ggtcctgcgt
    16561 gaaggagtaa ggcaattctt aaaaaatcac aagcatgtta aggaatcaag acttggcacc
    16621 ttcaacgaag gcggcgatgg tgttacaata gtaaaattca agtaaatgac aaggggacgg
    16681 gggtactgtc ataattcaga ggcggtagct gtcttaaggg attaaattat gaatgtacgg
    16741 gataaataca gtaataaagc tgtaataaag tatacgggag cttttatagc ttgggttatc
    16801 ggttcgggat ttgcaacggg acaagaagct ctgcagttct tttcaagtta cggatactta
    16861 agctatgggg ttcttctggt taatttgata ggttttttat ttttggggca agtattatta
    16921 gttacaggat ttgaacataa aaccgaaagt tcatttaatc attttaaata cttttgcggc
    16981 gagaaaatag gaacaattta ttcttggctg attccaatta ccttgatttt aattatgcct
    17041 gtcttaatct ccggtgccgg tgccaccctt tccgagtatt atggagtaaa tcgctatgtc
    17101 ggttcttttc ttatgtccgt cttggtttta agtgtctatt taattggatt tgaacattta
    17161 ataaaaatag tttcagccat aggacctgct attattattt tttccttgct tgtgggagcg
    17221 ataacactag caaatgatta cggcaatttt tctgatgtac caaaatatca atttaaactc
    17281 aacgagttgc aaacagctcc acattggacg ataagtgcta ttttgtactt atcgctgaat
    17341 ttcttaagcg gcagcgtata ttttactgag cttggcaaat cagcatataa tcgaaatgac
    17401 gtaaaatacg gagcattttt tggggctctt gcaataattt taaatattgc aattatgaat
    17461 actgcaatct tattgaatgc tgaaaacata tcaacattag caataccggt cttatattta
    17521 gcaaaaaaaa tatcttatat gttaggagca attttttcaa ttatattaat attaggtatg
    17581 ttttcttctt gttctgcaat gatgtggtca atatgcaacc gatttacaag aggcgggaaa
    17641 agaggcaaca ggatttttgc agtctttatc tcgatattaa cctttgtgtt agggttattc
    17701 tcgttcagtg aacttatagg taccttttat cccctcgtag ggtatgttgg attgtttttt
    17761 attggttctg taatatataa aggaattaag tataatatta ttaagaattg aggtgcataa
    17821 tatggaaaga ataataaaag atgtaaattg tcccaaatgc ggacaagtga tggaagaagg
    17881 atatattcat tcacgaaagc aaatattatg gtccgaagat gataagccaa gactgtttga
    17941 tttctatgat gaaaatttag tcacaatgcc tataagaaga gcaaataaag ttcccgcatg
    18001 gagatgcaag gaatgtaaaa ttgcaatatt ccggtatgaa taattatgat ggtataatat
    18061 ttagtaaatg ctaccattta tgattaatcc atgatacaat atttaataaa aaggtaaatt
    18121 tattattaat attaaataaa aataaaagaa agggagtgta aaagatgaga caatatgtag
    18181 ttgatgcatt tgcagaaaag gtttttgaag gaaatcctgc agcggtatgc attttggatg
    18241 agtggctgcc ggatgaagtt atgcttaata ttgccagaga aaacaactta gccgatactg
    18301 cttttgtggt taaggatggc acaaatttca aattaagatg ggttactccc ggcggtgaaa
    18361 tagatttatg cgggcatgca acccttgcaa ccggatacgt tattagcaga tttattgctc
    18421 ctgaattatc ggaagttgtt tttcaaacca aaagcagcgg aatattgaaa gtccttaaaa
    18481 aaggtgagct ttatgaaatg gattttcccg cttataaatt aaaaaaggta gaagttacaa
    18541 atgcaatggc ggatgctata ggagttaaac ctatagaagc atatatgggg agagatttac
    18601 tctgcgtact ggagtccgag gatgaagttc gaaatttaaa tccgaactta gaaaaggtaa
    18661 aattactgga cggattgctt tttcatgtta cagccaaagg aaatgaattt gattgtgtat
    18721 caaggtcatt tgtacctaag cttagcggta ccgaagaccc ggtatgcggt tcggggcatt
    18781 gccatataat cccttattgg gccgaaagat taggaaaaga caatttaatt gcttatcagg
    18841 catctcaaag aggcggtacc ttgtattgta aatatcaagg ggacagggtg ataatgggag
    18901 gcaaggtagc attgttttcc accgcggata taaatataga atagtttttt atttacataa
    18961 agaaaatatt attaagatga aacacataat atcgatatta aatatggagt acttatggaa
    19021 agagaaatag taagattgga tatggataga gattttgagg catttgaaga tttattgaaa
    19081 atctctttcg gtgaagcaac caactcaaag aaagaaatgt atgaatggct ttttgaaaat
    19141 aacccttaca ataaatccgg aaatatgatg tatttattaa aggaagggga taaggttata
    19201 ggttgtgacg gattgttgcc aaacgaactt tatgttaacg ggaaaacttt gttaaccgct
    19261 cattcggtta aatccatgac ccatcccgat tacaaaagac agggtatttt cagaaaaatg
    19321 acggaaaact cctgtgagag gggaaagcag gacggtgtgg atgtgataat aggtttggca
    19381 aacgaccaat cttatccggc ataccaaaaa ttcggctggc ctacactttt tgaaaaagaa
    19441 gtatatgtaa agcctatact tattaacaat attcttaaac gaagaattaa aattggtttt
    19501 ttgtcagaag cagccaattc tgtctatact gtatttatga agaataaact aaaaaatcag
    19561 atggacaaag aaattaaatt tgaaatactt gacagggttc cggaagaaat tcaaaaatgt
    19621 tgggacagat acaaatcaaa atataatgtt cttttggtca gagattataa atacctgaac
    19681 tacagatata acgaaagacc ggacattaat tatgtaacca ttcttgcaag gcataagggg
    19741 gagattatcg gctttgcaat acttcataat gcagttgcaa acggctctaa tatgacatct
    19801 gctgtagaat tttttaccga ccccgataat gaaagataca taaaagcatt ggctaatggc
    19861 atagcgaagt attgctatga caagggactt gaatatgccg ttattggaac ggggcattac
    19921 ggtgaataca ggaaggttct tttgaataat ggatttatgg taacccgcaa acctccaaag
    19981 aataatatga tgattgctca tatattatct gacaaagtaa ccttagagga aataacaggt
    20041 catgaaaagt ggcatataac tcaaggagac ggcgaaacag agctagactt ataactgtat
    20101 tgtatccgtt tttttaagag gttttcaaaa tagcagtaaa atactcctaa tacgcctcta
    20161 taattctttt aaagcatctt ggtcaggaaa aaccaagctt tggtttttcc tgagttcttt
    20221 tatatttata agcaccactc cggaatttat gtaggtgctt ttctcaggca tatcaagcct
    20281 aagctgcaaa taatgtttgt ttacagttaa aattcatgtt aaaatatagt ttgagattat
    20341 atatataata caattattca tatgtaatgg ggagaaggta ataatgatta aattaatagt
    20401 ggacagcaca tgtgatttga attgtgaaat tttggacaac catgatgtag aggttattcc
    20461 gctaagtatt actattgatg acaaaaccta tttagatgga gtcgatataa ctacggacag
    20521 agtttatgag tatatgaaag acggtaaagt ccccaagact tcacaagtgt catttgagtc
    20581 tgtatcccaa gccgttgaaa agtgtatttc aaaagaatca gattttattt atttggcttt
    20641 ttcatccaaa ttatcaggca catacagttt tgccaagaaa ataatagata tgtataagga
    20701 aaaatatcct gagagaaaaa tggcaataat tgattctaaa ggcggagccg gagggaccgg
    20761 tttaatagct atacaagtac ttaaaatgat tgaaagaaac ttaccctttg aatatatctt
    20821 aaaccaggca caattcatgg ttcaacatat agtttacaga tttacaatat ctgatttaga
    20881 atggctgtca aaaggaggca gaataagcaa gtctgcagga tatgtaggaa atgtattaaa
    20941 tattaaacca tatctcgttt tagatgatgg acttattaaa gtatccaaaa tgattcgcgg
    21001 cagcagaaaa actattcaaa ctttgattca agatattgaa gacggtacag ataaatttaa
    21061 aaatcagatt atcggcataa gtcatgcgga tgatttagat actgctttaa tggtggaaca
    21121 aaaaattaag gattcaatta aaggatgcaa gacaacaatt tttaaaatcg gtgccgtttt
    21181 aggagttcat ttgggaatag gcggtgtagg agtttttttc tttgatgaaa aacctgaatt
    21241 ctatgaatta taatattatt aatagtatat aaaaatagtt attgcattag ttgacacatt
    21301 aaaaatgctg tgatattatt acaaaaaata gaagttacaa cctgccgaac atatcaaatg
    21361 gtaggtttta gttttaataa ttcgtaatta ccttggagga gagagatgga ttttaaggaa
    21421 aaagtggctg aaatttttac atccatagaa accgagctgt caatggatga tgctcttcaa
    21481 ttaatagaga taccacccag tagcgatatg ggagattatg cggtaccgtg ttttaaattt
    21541 gcaaagaagt acaggaaatc tcctgcagca atagcaaatg aaattgtaga aaaaatagga
    21601 gcagttgaag aattcgaaaa aattgaaagt gcaggaccat atgtaaattt ctttgtaaat
    21661 aaatctcagt ttgcaaaaaa aatacttaaa caagcttttg aagaaaaaga aaattacggc
    21721 agaacgaatg tgggtgaagg caagactgtt atagttgagt tttcatctcc gaatattgca
    21781 aaaccgtttc acatagggca tatacgctca acattaatag gaaatgccat aaataatatt
    21841 tataaatacc ttggttataa tactgttgca ataaatcatt tgggggatta tggaacacag
    21901 ttcggaatgc ttatttcggc atataaaaaa tgggcaactc ctgaaataat tgaagcaatc
    21961 gaaaaggacc ccatacctga acttttaaag ctgtatgtaa gatataatca ggaaattgaa
    22021 acacatccgg aataccagga agaagcaagg tactggttta atcaattaga aaaaggcaat
    22081 caagaagcat atcaattatg ggcatggttt agggaagtta gcttaaaaga atttaaccgc
    22141 gtatatgata tgttcggtat taagtttgat tcatatgccg gagaaagctt ttattcggat
    22201 aaaatgcctg caataatcga tgaattggaa gataaaggac tgcttaaaga ttcggaggga
    22261 gcaaaaatcg tagagctttc cgagtttggt ctgacggatg cattaatcca aaagagcgac
    22321 ggcgctactc tatatgtaac cagggatgtg gcagctgcaa aatacagaaa agaaacatat
    22381 aatttttaca aaaatatata cgtggttggt gcacagcaaa aattacattt tgaccagtgg
    22441 agaaaaataa tagatttaat gggatatgac tgggcatatg actgtgtaca cgtaatgttt
    22501 ggaaccgtaa gcctggaagg cggtgctata gctacaagaa aaggaagagt agtatttttg
    22561 gaagatgttt tgaataaagc aattgaaaaa actaccgaaa ttattaatga aagaaatccg
    22621 ggcttggaag acagggagaa agttgcaaag caggttggta taggtgctgt aatattccaa
    22681 gagcttttca ataacagaat aaaagactat gtattttctt gggataaaac attaagcttt
    22741 gaaggtgaaa caggacctta tgttcagtac acatttgcaa gaacctgcag tcttcttaga
    22801 aaagcagata ttgatataaa tgatgatgtg gattattctg ttttgacaga cagtgaatca
    22861 ttcaatgtaa ttaaaaaact ggaaggtttt aaagaggcgg ttttaaacgc tcatgataaa
    22921 tacgagccct ttatgattac ccgatatatt gtcggccttg cacaagaatt taaccgattc
    22981 tatcatgaat gtcagattat tactgacgat gaaagtgtta aaaaggcaag actgcttctt
    23041 actctcacag taaatacagt tcttaaacaa ggtatgaagc ttctgggaat ggaagcaccg
    23101 gagaagatgt aactacgtaa acttcggtgc cagg
//
LOCUS       DAOPUE010000022        22839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2258_length_22839_cov_10.496401, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000022 DAOPUE010000000
VERSION     DAOPUE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 22839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22839
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2258_length_22839_cov_10.496401"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1600)
                     /locus_tag="PKH42_03490"
     CDS             complement(128..1600)
                     /locus_tag="PKH42_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19526.1"
                     /translation="MKNKISIKLIVYFSAALIVFSIIIGNIFMLLFKNNAVEIHKNEL
                     KRRALTIADTISELMDGSSLNFGPGMGRHGGMMGMQGGLGLYIRNLDDIAMADAWIVD
                     EELNLLITGSMSHIQYNYTDLPQDADIVVKKVFEGQTTFSEGFSKLLETPTLTIGTPI
                     TSDGQIIGALLIHSPIQGINDATSEGFRILLISIFMALILSVLLSVALALTFTKPLEK
                     MKNTAALLAGGNYSVKTGIKQNDEIGDLAKTMDILSQRLGKAEEERKRVQKQRQDFIT
                     NISHELRTPVTVIRGSLEALSDGVITDSDKINEYYKQILIESKSLERLINDLLELSRL
                     QNVDFKIEMQELNICDVLKDSLRSASNLARQKNINIQYNSDKDVFLTKGDYGRLRQMF
                     LTVIDNAIKFSRENKSIYITLKDNQITIRDEGIGISEKDLPNIFDRYYSVKSDENKTG
                     TGLGLTIAKQIALRHDIELFAESKINEGTSFVFVIRSNFE"
     gene            complement(1590..2279)
                     /locus_tag="PKH42_03495"
     CDS             complement(1590..2279)
                     /locus_tag="PKH42_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA19527.1"
                     /translation="MPKMLIADDNRQITSILEEYAKKEGYDVKIAYNGRQALDTFDKH
                     KPDIVLLDVMMPIIDGFEVCRRIRKVSNVPLIMITARGEDFERIMGLDIGADDYIVKP
                     FSPAEVMARVRAVMRRIGAENISSKLFVYDNIKINMEDYIVTIDENNVSLTKKEIEIL
                     WTLAVNKNKVFSRENLLNSLWGYDYYGDSRTVDSHIKRLRSKIDNFQHKNWEIKTIWG
                     VGYKFEVYDEK"
     gene            2447..2833
                     /locus_tag="PKH42_03500"
     CDS             2447..2833
                     /locus_tag="PKH42_03500"
                     /inference="COORDINATES: protein motif:HMM:NF022378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HOA19528.1"
                     /translation="MKNKILIIGLVLLIILATSITVFAITGRTQTAYDESEDTWEKFR
                     DEMFENKKAFIEERVKDGTLTQEEADEILKNIELMQEYCLGAGGCGMGRGFGMMRNRL
                     GNNSGYGNYGFRGMGFGGGRCGRGSW"
     gene            2951..3583
                     /locus_tag="PKH42_03505"
     CDS             2951..3583
                     /locus_tag="PKH42_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA19529.1"
                     /translation="MYNYYNYPCQNIYPWYRQQDRMYNRQRMYNPYNNLMNPDFNNYP
                     RYPTYPDYPYNQESFYSPWSLQYPSDRGFIEIKDYGPNPFVINIEEATEQNNTFRSTL
                     WTGDHLQLTLMSLNPGEDIGLEMHPDVDQFIRIEEGRGIVQMGDSEDNLDFEEMVSED
                     FIFIIPAGKWHNLTNIGRTPLKLYSIYAPPEHPHGTVHETKAEAEAVHGN"
     assembly_gap    3897..3996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3997..5671)
                     /locus_tag="PKH42_03510"
     CDS             complement(<3997..5671)
                     /locus_tag="PKH42_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19530.1"
                     /translation="MNKLGKDCMPCTVKRSMAIILTLMMIISSLLSSGNLIYAYGDGE
                     LVLKSASHPEAVREAVIDGNKVTFTLPSGFASETLDLKDIICELKTEFNQEKIIFPSG
                     SDAEIDGPTVEMEVKYTYKEGEDTTTEYSTNYYIEVKSGEFPTFSGTISKTTTINDNI
                     IFKSSDFTTSYEKNDGEEMGFISINTADIAVGTLEYNGSAYVAGSQISVSDISKLIFK
                     PTNIGKASFIVTAFADDEAKTDCGYATLNMEVIYPAASDIKLSTNQDKVLKLSSSQFV
                     DACNKTVGGTFDYISFDSMPSNGKIYIDYVSSTNKGTAVEAGKEYSKADLDKMSFVPN
                     SGYFGKFTIPYKGYNTAGDVFTGKVEITVAEKVFVIDKIKYSLYVNTLVDFNADDFEE
                     VCLDGTGQRLNYVKFTLPSSSRGTLYSNYGLSSQSKVSSTSKYYDDDIDDISFVPYKN
                     YSGTVVISYTGYNSKGESYTGEIEIQVLKVVTDADDITYKTGSYTPIDFDADDFDDEC
                     RDVTGKRLDYVRFTIPSSTYGTLYYNYSSRSQSKVSSTTKYYDDDLDDIT"
     gene            complement(5837..7555)
                     /locus_tag="PKH42_03515"
     CDS             complement(5837..7555)
                     /locus_tag="PKH42_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOA19531.1"
                     /translation="MKRTRLKYLIISLTLILLFYIGQAASFLTEGSEKTKSINENTEK
                     AQQIIIGYYPAWKSYSGYTPESVDISKLTHINYAFANIGPDYRIEMGYPDKDPENFEK
                     FRDLKKINPDLKVLISVGGWNWSGRFSDMAATDENRNKFTDSCVEFISKYGLDGVDLD
                     WEYPVGGGLKTNSKRPEDKQNFTLLLKEMREKLDAQELKDNKDYLLTIAAGASNYYIE
                     NTEADKFQNYLDYVNLMTYDIHGPWDKYTDFNSPLYNNNDESHQYKISIESSVKAWLR
                     AGLPAEKLVVGVPFYGYLYTVSKNTIDGLYQTHKNGKALSYDTIKKEYLSNPQYTKNF
                     HYESLVPWLNNGNTYISYDDSQSIALKAEYIREQNLGGAMIWELSQDSEGELLNSLYE
                     GLYDGSVLAARDYVGHWAEAVIQKWLDMEYITGYPDGTFRPEEFITRAEFVKIVNNAF
                     GFKETAEITFTDVKEEKWYYEEIQKAYKEGDIVGVSETEFAPENYITREQAAIIMTRL
                     LELEGNEKGAVVFSDSSKVSSWAKEYVGAAAEHNLIKGYEDNTFRPQNNIKRAEALVL
                     LDRILK"
     gene            7806..9158
                     /locus_tag="PKH42_03520"
     CDS             7806..9158
                     /locus_tag="PKH42_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA19532.1"
                     /translation="MRNNIILTEGKIRSALVKLALPIMGTSFINMAYNLTDIMWLGRL
                     STDAVAAAGAAGYFLWFGSSLVMIAQVGVGVMVAQLFGKGDSEGVKNYINNGLQLNLF
                     IAILYSLFLYIFRHQIIGFFNIENENVSKMAVEYLTIISAGITFHFLNPVFSAILNST
                     GNSITPFKINTVGLIANIVIDPLLIFGFGPLPALGVKGAALATIMAQFFVTMIFLIIG
                     KRYDTIYSHAKLFKKPRLPIIQNIVKIGFPPFTQMGIHAGIAIILTRILAVFGPVAIA
                     VQSIGAQIESITWMTTEGFSAAISAFVGQNYGAGKIKRVKDGYVQGIQIVGSIGIFST
                     LLLIFGAEQLFSLFTPNDPIAIKEGIIYLHILGFSQFLMAIEIGTTGAFNGLGKTIFP
                     TINGIILNVLRIPAALILSRSLGLPGVWWAIAISSNLKGLILFILFYRYSNNFERTYI
                     "
     gene            9437..10360
                     /locus_tag="PKH42_03525"
     CDS             9437..10360
                     /locus_tag="PKH42_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOA19533.1"
                     /translation="MAIRPPILRAGDTVGVVTLGSPRDAESVDSRIQTLESMGFNVVV
                     GEFVYSDAGIVAASPMERAADLMNMFVNPEVRAIIPTRGGTGVSDIIPYLDFDVIRRN
                     PKIITGYSDITILLNVLYQLSDLITFHSLLLIDFRPTTPAYNFDQFFTATSTITAPRP
                     LENPPGIPLISRIEGNVTGTIVGGNLTSFVDTLGTPFEIDTTGRILFIEETHEAASRV
                     YRMIAHLIHAGKFRDCAGVIMGECAGCYANYGKTYEDIINEVIVPLGKPLMTNLASGH
                     GYYKMAIPIGAVTNLNTYNNTLTVLEPTVSL"
     gene            complement(10377..12800)
                     /gene="ligD"
                     /locus_tag="PKH42_03530"
     CDS             complement(10377..12800)
                     /gene="ligD"
                     /locus_tag="PKH42_03530"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HOA19534.1"
                     /translation="MAVGLNEYKQKRNFEKTFEPEGKTQSSEESLRFVVQHHLASRDH
                     YDLRLEWNGVLLSWAVPKGPSYNTKDKRLAVQVEDHPLEYRNFEGTIPKGEYGGGVVM
                     IWDEGYWEPHMNVDEGLSEGMLKFSLKGSRLRGRWALIRLKGKAGETQDNWLLLKEKD
                     EYSKDEEGMSEFTTSIRTGRTMAEIEGGQDEKFIKNPFSKADVQLAKLVKAIPEGDWL
                     YEMKYDGYRILAFVEGNSVRLITRKGNDITKRFFTIASSLIDLAKGRAMVLDGEIVIT
                     DESGKTDFQALQNYLKTQNSQNLTYIVFDLLAIDGEDIRGQSLILRKEKLESLLQDAP
                     KNLYYSRHVKGYGKESFAAACEAGMEGIVGKKPDSIYSGKRNDDWIKIKCDKRQEFVI
                     GGYTLSDKRSSGVSSLLLGVYEGEDLIYAGRAGTGISENDMKILESKFEGLKCSKPPF
                     KEVPKTKPNEKITWLEPKLAAEIKFAEWTKDNLLRQASYKGLREDKDTKDIKREKAEG
                     ILPQSNDEEGRYMEKDLIIEGVKISSPDKIIYNELDITKEEVIRYYSKVADKMLPYVR
                     HRILSIVRCPKGVSQACFYKKHPGPGSKGIITMPVSTGDDETGEYFYIENATGLISEA
                     QMGTLEFHIWGSSVDNLEMPDMMVFDLDPDEGMDLDKVRQGVIDIRSILDELNLKSYL
                     KISGGKGYHVVIPLKPTADWDKFHDFARQVAEVMENKWPERYTSNVRKAKRKGKIFID
                     WIRNARGATSIAPYSLRARKGAKVSMPIFWEELYTVAPDGINMEDALLRIEKKDPWED
                     FFQNSQSII"
     gene            complement(12802..13572)
                     /locus_tag="PKH42_03535"
     CDS             complement(12802..13572)
                     /locus_tag="PKH42_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOA19535.1"
                     /translation="MISRKSVITFGMVAIPIAMYTATQDNDIHFNQLHAEDNSRIRYK
                     KTCAHCGKEITSNEIVKGYQYDKDKYVVITDEEIEKIKTEKDKSIQILHFAELNQISP
                     VYYEKTYQAVPEPGGEKAFELLRSALMAEQKIAIGKTVMGTKDTLMAIIPREEGILIS
                     TMFFADEVKEIQKQYTKPEVSKQELDMAKLLINSMDTPFDPSKYKDEYQVKLRELIEA
                     KISGREVVAAEPESAGKVIDLMEALKASVEKAKNKETA"
     gene            complement(13698..14441)
                     /locus_tag="PKH42_03540"
     CDS             complement(13698..14441)
                     /locus_tag="PKH42_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOA19536.1"
                     /translation="MEAIFRKKYIVQPSETDFRRQLKLSSLFLYLQDMATEHAYNIGV
                     GRDVLQKKYGIIWVLNRVKGEVIRYPMFKEEITIETWPEPPEKVQFNRNFLVYDKDGN
                     IIVRAFSQWVVIDFITRKLRRSSIIEEEFPKVDRERAIDCTLNKIKPRGKLNLGYKKT
                     VGYSDIDINEHLNNAKYIDYIMDCFSLENHKKYSVKAIDIDYIHEALPGEIIQLYTDT
                     EDAKDNMVYIEGINENTKLTTFKAQILRK"
     gene            complement(14469..15860)
                     /gene="argH"
                     /locus_tag="PKH42_03545"
     CDS             complement(14469..15860)
                     /gene="argH"
                     /locus_tag="PKH42_03545"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOA19537.1"
                     /translation="MKNLWGGRFSKSMEDYTADYNESISFDNILYKHSITGSKAHVKM
                     LAKQQIISNEICEEILRGLSIVEKKIENNEVEFNILDEDIMMIIEKELTKEVGEAGKY
                     LHTARSRNDQNALDETLFLRDAVKNSIKHLIDLLDVLINKAEEESHKIMPGFTHLQHA
                     QPITVGFYYMAHFQGLRRNVERFRELLKRLDKNPLGSCALAGTTLPIDRFATTELMGF
                     SEPTENALDTVASRDNIAEYLFNAALCMTHLSGFAEEIIVFNSQEFNFITIDDSFCTG
                     SSIMPQKKNPDIAELARGKSGRAIGNLITLLTVLKGTFLTLNKDYQEDKEALFDSIHT
                     LDRTVFIFARMLENITFNEDVLRAHLKKGFIEATDIAEFLVKHNIPFREAHEIVGKLV
                     KYCELKSKTFQDINKEELKELDFPVEIEDLSQFTVENCIKNRNSYGGTSYDDVKRQIN
                     NAKNFVAELKSLF"
     gene            complement(15874..17088)
                     /gene="dpaL"
                     /locus_tag="PKH42_03550"
     CDS             complement(15874..17088)
                     /gene="dpaL"
                     /locus_tag="PKH42_03550"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOA19538.1"
                     /translation="MEIYEACGIKAVINNKSKGVRPYFLSEEEIKGVRKFHATIDGYE
                     ITPLVSLECLAEKLGVSKIYVKDESKRFGLNAFKGLGATYAIARLLCEKLGVDIDSID
                     YNYFKLPVVKEKIKDMVFVTATDGNHGRAVAWAAAQLGCNSIVYMPKGSSLRRLQHIK
                     DTGAEAFITDVNYDDTVRMAKKKADEIGGLLVQDTAWEGYEKVPNWIAQGYTTMAAEA
                     IDQMKSDGSMKPTHVFLQAGVGSFAGGILGYFTNIYKDEPPVTVIAEPENAACIYKSA
                     LADDGKPHSVSGDLNTIMAGLACGEPNTVTWPILRDFAKVYVSCPDYVAAKGMRILAA
                     PSGNDKKVISGESGAVGLGLLTLFLEKEELKPLKEKLKLNENSVIICFSTEGDTDPEQ
                     YRAIISQPEIFN"
     gene            complement(17245..18408)
                     /locus_tag="PKH42_03555"
     CDS             complement(17245..18408)
                     /locus_tag="PKH42_03555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA19539.1"
                     /translation="MKHKFIAKRYWKDKSTAMGQSDVIAKSFEDIINLSLGDPDLITH
                     DIIIDNSYADAKAGHTKYTDFRGDPELRQEIINYYKEEYDMDIKDEEVFVCASGCLGM
                     YLALEAIVDDGDEIILQAPCFTPYPQQVELARGVPVELPTYEEEDFQINVDRLECLIT
                     EKTKALVINSPSNPTGSCLTVEVMEKIAQIAEKYDLIVVSDDIYTSFSYQYPFVPFAS
                     LPGMKDRTIIINSFSKNFTMTGWRIGNIIAPDYIIKVIQQINENVVFTAPSISQRAAI
                     YALKNRHIIQPPMIEEYKKRMFYAAERINKIPKLSVIYPPKGSFYLFMNIKKTGLSSV
                     EAADLILKEAHVLMLPGDAFGACGEGYIRIACTVDVETLKEAFDRIEKIALLN"
     gene            complement(18534..19292)
                     /locus_tag="PKH42_03560"
     CDS             complement(18534..19292)
                     /locus_tag="PKH42_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOA19540.1"
                     /translation="MTLFRWDELTWTELTNLRDGIIIIPTGSVEQHGPHLPIGTDTFI
                     AEGFAKKLYNKAIDKKIIIAPSISYTYAKLNSNYLGTINLNGSTLINLGLDLISEFLR
                     QGFNKIILINGHLESIPFLMEGIELALTAYENSKNTNSEIPKIVIANWWEFISDEMID
                     NIFGDKWPGWEAEHAALTETALMKYLYPELVRDIKVDSCEYEKLPYRILPWNKRVQPS
                     SGSYANPDGATMEIGRHLTEAVISGFTELIKKEF"
     gene            complement(19279..19785)
                     /locus_tag="PKH42_03565"
     CDS             complement(19279..19785)
                     /locus_tag="PKH42_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19541.1"
                     /translation="MDIVCLQTNAEYNSIKNEAADFKDYDCQEIFYPLWLLEVEVCMH
                     LLFSKVKRMSMKVAVDGLTGDISFICKQIETYHAEVKPEQLLPVTAKMDAEGLAKVKD
                     SLLPFVLSKARSFLVRPSINIQNKSIIFKPFYRLSSMDGDDRIIYLDKSNGKIAVLRQ
                     EGKSNDSF"
     gene            complement(19798..21405)
                     /locus_tag="PKH42_03570"
     CDS             complement(19798..21405)
                     /locus_tag="PKH42_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOA19542.1"
                     /translation="MKLNVIIPFVSYLLIMLGIGMYAMRRIKTYDDFIIGGRSIGAWV
                     SSFALITSYASGYTYTAAPGMSYTGGWSTMWWSTGDAPGNALSFGLLGRRLRKFSELL
                     GAITLPEYYEKRFKSPGLRLLTSIIIITTVSLHLVAQWQASGILLSVTFGTDYLVGML
                     IGGAVVLAYTIMGGYLATVYTDFMQGVVMFIGTHILFWSAISKVGGFNALNDQLALIN
                     PGLVTPWGPDGSYFGLIAAASPIILIIMGSFGMPHVTVRHLSLKNPDTARKAMLITAI
                     FVALFSFAYYMVGAAGLILLGPGLENPETSGVLLWFEVLPPVGAGILVSAAVAAIMST
                     ADGFLMLIVSTIAHDILNRFLLPNTPQEKRIRIARIITAIIVILSLVAAINPPAGVFT
                     IVMTVFGGMALAFGIPNIFSVYWKRSTKTGAIACVILSLTVYVWLTAAGIELFGMSPF
                     MNGLIVAILAFIIGSLLTQKPGPEMEELFDIGTSYGPLPESFVKSSNVSEELKAEALR
                     AEKLMTKNNVLKQSLNINALNEPIPTN"
     gene            complement(21435..21575)
                     /locus_tag="PKH42_03575"
     CDS             complement(21435..21575)
                     /locus_tag="PKH42_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19543.1"
                     /translation="MEGSKLFWSSGTWIFFLVQALVVALAVYLYWYLGKKEKEACEEK
                     KE"
     misc_binding    complement(21772..21981)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(22121..22828)
                     /locus_tag="PKH42_03580"
     CDS             complement(22121..22828)
                     /locus_tag="PKH42_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HOA19544.1"
                     /translation="MKKFIIEDDFWELFPDAKFGVVICHNIDNKIKEVDKYLDMLRDS
                     EDEALKYLDNPEFIQNDVIKVWREAFQKFKTKKGARCSIEALLKRVKNGNHIGTINPL
                     VDIYNSISLRYALPCGGEDIDAFAGDNRLTKAVGDESFITYGSDKSEPPYEGEIVYKD
                     DQGAICRCWNWRESVRTMLTESTKNAFLVIESVDEKRTEELKNAINDLAKLVQDNLGG
                     TIKTSILDINNKLIDLE"
BASE COUNT         6647 a         4680 c         3555 g         7857 t
ORIGIN      
        1 tcgtcgcaca aaattggcac tcgttgcgaa tgacctaccg ctatttttct ctccctttag
       61 tcggaaaaat agggttaggg catctttatt atatattttg ttaattaaat tttcaataaa
      121 atataattta ctcaaagttg ctacggataa caaatacaaa acttgttccc tcattaatct
      181 tgctttctgc aaaaagctct atatcgtgcc ttaaagctat ttgttttgca atagtaagtc
      241 caagtcctgt acctgttttg ttttcatctg attttacact gtaatacctg tcgaatatat
      301 tgggaaggtc tttttcggat atgccgattc cttcatctct tatagtaatt tgattgtctt
      361 ttaaagttat ataaatgctt ttgttctcac gtgaaaattt aattgcatta tctataactg
      421 tcaaaaacat ttggcgaagt cttccgtaat ctcccttggt taaaaacaca tccttatcgg
      481 aattatattg aatattaata tttttttgtc ttgccaagtt tgaagcactt cgtaaagaat
      541 cctttaatac gtcacagata ttcagctctt gcatttctat tttaaagtcg acattttgca
      601 gtcttgacaa ttccaataaa tcgtttatca atctttccaa gcttttgctt tcaataagaa
      661 tttgtttgta atattcatta atcttatccg aatcggttat tactccgtcg gaaagcgcct
      721 caagggagcc ccttataaca gttacgggag ttcttaattc gtgagaaata tttgttataa
      781 aatcctgcct ttgtttttgc actctttttc tttcttcttc agctttaccg agcctttggc
      841 tcaatatgtc cattgtttta gcaaggtcac ctatttcatc attttgttta ataccggttt
      901 ttacgctgta attccctcct gccagcaggg ctgccgtatt tttcatcttt tcaagcggtt
      961 tggtaaatgt taaagctaag gcaacagata aaagtacaga taaaattaat gccataaaaa
     1021 tacttattaa taaaatccta aaaccttcac ttgttgcatc atttatacct tgaataggcg
     1081 aatgtattaa aagtgctcct attatttggc catcagaagt aatcggagtg cctatcgtca
     1141 aggtaggtgt ttccaaaagc ttgctgaacc cttcactgaa agttgtttgg ccttcaaaaa
     1201 cttttttaac aacaatatct gcatcttgag gcaggtcggt ataattgtac tgtatgtggg
     1261 acatgcttcc cgttattaaa aggtttaatt cttcatcaac aatccatgca tctgccatgg
     1321 ctatatcatc caaattccgt atatataaac ctaagccgcc ctgcattccc atcatgccgc
     1381 catgtctgcc cattccgggt ccgaaattta aactgcttcc atccattaat tccgaaattg
     1441 tatctgcaat tgtaagagcc cgtcttttta gctcattttt atgaatttca actgcgttat
     1501 ttttaaacaa aagcatgaat atattgccta taattattga aaaaacaatt aatgcagctg
     1561 aaaaatacac tatcagtttt atagaaattt tatttttcat catatacctc aaacttgtag
     1621 cctacccccc atatagtctt aatctcccaa tttttatgtt ggaaattatc gatttttgag
     1681 cggagtcttt taatatggga atccactgtc cggctgtccc cgtaataatc atatccccac
     1741 agactgttta acaggttttc tcttgaaaaa actttgttct tattaacagc caaggtccat
     1801 aatatttcta tttccttttt cgttagggaa acattatttt catcaatcgt aacgatataa
     1861 tcttccatat ttatttttat attatcatat acaaataatt tactgcttat attttcagcc
     1921 cctattcttc tcataacagc tctcactcta gccataactt cggcaggaga aaatggtttt
     1981 acgatataat cgtccgcacc tatatcaagt cccattattc tctcaaaatc ctctcctctt
     2041 gctgtaatca taataagagg aacatttgaa actttcctta ttctacggca tacttcaaac
     2101 ccgtcaatta tcggcatcat aacatcaagc aagactatat ccggtttgtg tttatcaaat
     2161 gtatccaagg cttgcctgcc attgtaagca attttaacat cgtagccttc tttttttgca
     2221 tattcctcca atatggatgt tatttgtctg ttgtcatctg ctattaacat tttgggcata
     2281 taaaaatacc tcctatgttt tttgtaatta taccatatgg taataggaaa atgtgacaat
     2341 tatgtgtttt tataaaaaga aaaatcataa tttttttata ctaatgacat aaatttgaca
     2401 caaatattgt ctatattaaa tctaaagaaa attcaggagg taaaaaatga aaaataaaat
     2461 tctaataatt ggtttagtat tattaataat attggcaaca tcaattactg tttttgcaat
     2521 tacgggaaga actcaaactg cttatgatga aagcgaagat acttgggaga aattcagaga
     2581 tgaaatgttt gaaaacaaaa aggcttttat agaagaaaga gttaaagacg gaactcttac
     2641 tcaggaagaa gccgatgaga ttcttaaaaa cattgaatta atgcaggaat actgccttgg
     2701 agcaggcggc tgcggaatgg gaagaggctt tggaatgatg agaaataggc ttggtaacaa
     2761 tagcggatat ggcaactacg gcttcagagg tatgggcttt ggcggaggca gatgcggcag
     2821 aggttcttgg tgagatcctt cgctgacgct caggatgaca taatgtatgg ttgatgtcat
     2881 attaaaatca cattctttgc atagaaaaca tattatagta ttaataaaat acagaaagga
     2941 atttgaaaaa atgtacaatt attataacta tccatgccaa aatatatacc cttggtatag
     3001 acaacaagat aggatgtata atcgccaaag aatgtataat ccatacaata atctaatgaa
     3061 ccctgatttt aacaattatc ccagatatcc cacttacccg gactatcctt ataatcagga
     3121 atctttttac agtccctggt cattacaata tccgagcgac agaggcttca tagaaataaa
     3181 ggattacggt ccaaatccgt ttgtaatcaa tattgaggaa gcaacagaac aaaacaatac
     3241 tttccgttca actctatgga caggggatca tttgcaactc actttaatga gcttaaatcc
     3301 cggagaagat ataggtttgg agatgcaccc tgatgttgac caatttatac gaattgaaga
     3361 aggtcgggga attgttcaaa tgggagacag tgaagataat ctggattttg aagaaatggt
     3421 aagtgaagac tttatattca taatacctgc cggcaagtgg cataatttaa ccaatatagg
     3481 aaggacaccc cttaaactat attcaattta tgcaccgccc gagcaccctc atggcacagt
     3541 ccatgaaacc aaggctgaag cagaagctgt gcatggtaac taatattttt caataataaa
     3601 gcggtttctc ccaaaggaga aaccgcttta cctttgacaa actttagata cttcgggcta
     3661 gccctcagaa tgacaaccag tgtcattcta tgaatacacg aaagtgtcat cctgagcgta
     3721 agcgaaggat ctcatattta attattgaaa tttttcactg cgctcagtat gacaggcttc
     3781 gcctaagatt gttattaatc taattgtagg accttgtaaa gtattaccgc catttcagct
     3841 cttgaaatca ttgattttgg atataaatat gaattgtttc cttgaattat gcctgcnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt tatatcatcc aaatcatcat
     4021 cataatactt ggtggttgaa gaaactttgg actgacttct tgatgaataa ttgtagtaca
     4081 aagttccata tgtagatgaa ggtatggtaa atctgacata atctaatctt ttacctgtaa
     4141 catcacggca ttcgtcatca aaatcatccg catcaaaatc tattggtgta tatgagcctg
     4201 ttttgtatgt tatgtcatca gcatctgtaa caacctttaa tacttggatt tcaatttcac
     4261 ctgtatagga ttctccctta ctattataac ctgtataaga aatcactacc gttccggaat
     4321 aatttttata cggtacaaag gaaatatcat ctatatcatc atcatagtat tttgaagtcg
     4381 aggaaacttt agattgactg cttaagccgt agtttgaata cagagttccc cttgatgaag
     4441 atgggagagt aaacttaaca taatttaatc tttgcccggt tccatccaga cacacctctt
     4501 caaaatcatc tgcgttaaaa tccactaatg tatttacata taaagaatat ttaattttat
     4561 ctattacaaa tactttttct gcaacggtta tttctacttt gcccgtaaac acatcaccgg
     4621 ctgtattata acctttgtat ggtattgtga attttccgaa atacccggaa ttaggaacaa
     4681 aagacatttt atctaaatcc gctttactgt attctttgcc ggcttcaaca gctgttccct
     4741 tatttgttga agatacataa tcaatataga ttttaccgtt tgaaggcatt gaatcaaaac
     4801 ttatgtaatc aaaggttcct cctactgttt tattgcaagc atcaacaaat tgggaggagc
     4861 ttaatttaag aaccttatct tggttagtgc ttagtttaat atctgaggca gcgggatata
     4921 taacttccat gtttaatgtc gcgtagccgc agtctgtttt tgcctcatca tctgcaaagg
     4981 ctgttacgat aaaggatgcc ttacctatat ttgtaggttt aaaaataagc ttgcttatat
     5041 cagaaacact tatttggctg ccggctacat aagcacttcc attgtattcc aatgttccaa
     5101 cagcaatatc tgctgtattt attgagataa accccatttc ttccccatca tttttctcat
     5161 aagatgttgt aaaatcggag cttttaaata ttatattatc attaattgtt gtcgtctttg
     5221 aaatagttcc actgaatgta ggaaattctc cgcttttcac ttcgatataa tagttggtgc
     5281 tatattcggt tgttgtgtct tctccctctt tataagtata ttttacttcc atttctaccg
     5341 taggaccgtc tatctcagcg tcactgccgg aaggaaatat tattttttcc tgattaaatt
     5401 cagttttcaa ctcacatatg atgtctttta aatctaatgt ttctgaagca aacccagatg
     5461 gtaaagtaaa agtaaccttg ttaccgtcta tgacagcttc tctcactgct tcaggatgac
     5521 tggccgattt tagaaccaat tccccatcac cataagcata aattaaattg ccggagctta
     5581 acaatgatga tattatcatc atcaatgtaa gaattatcgc catacttctt ttaactgtac
     5641 atggcatgca gtctttccct aatttattca tagttacccc tcactttatc tttaatatta
     5701 tatttcatgc atataaccta aataattaga aacataaaat atacctaaat atattgtagt
     5761 acaatttttg attattagca atgggaaaaa ggaaaaaggt gcctggcacc gtggtgatgt
     5821 ggtgcctggc acctttttat ttcagtattc tgtcgagcaa gacaagagct tcggctcttt
     5881 ttatattgtt ttgcggtcta aatgtattgt cttcataacc cttaatcaaa ttatgctccg
     5941 ccgctgcacc tacatattcc tttgcccagc tgctgacttt gctgctgtcg gagaatacaa
     6001 cggcaccttt ttcatttcct tctaattcaa gcagtcttgt cataattatt gctgcctgct
     6061 ctctagtaat ataattttcg ggagcaaact ctgtttccga aactcctact atatctcctt
     6121 ctttgtatgc cttttgtatt tcttcatagt accatttctc ttctttaaca tccgtaaatg
     6181 taatttcggc agtttcttta aaaccaaagg cattattaac tattttgaca aactcggctc
     6241 ttgttataaa ttcttcaggt ctgaaagtcc cgtcaggata tccggttata tattccatat
     6301 caagccattt ctgtattacg gcttctgccc aatgtcccac atagtctctt gccgccaaca
     6361 ccgagccgtc atagagacct tcatacagtg aatttaaaag ctcgccttct gaatcctggc
     6421 ttaattccca aatcattgca ccgcctaaat tttgctctct tatatattca gccttcaatg
     6481 ctatcgactg agaatcatca tagcttatat aagtatttcc gttgttaagc catgggacca
     6541 atgactcata atgaaaattc tttgtatatt gagggttgct gagatattcc ttttttatgg
     6601 tatcgtagct caatgctttg ccgtttttat gagtttgata gagcccatca atagtatttt
     6661 ttgataccgt gtataaataa ccgtagaacg gaactcccac taccagcttt tcagccggca
     6721 aacctgctct tagccatgct ttaacacttg attcaatgct tattttatat tgatgtgatt
     6781 catcattatt gttatacaag ggagaattaa agtcagtata tttatcccaa ggaccatgta
     6841 tgtcataggt cattaggttt acataatcca aataattttg aaatttatct gcttctgtgt
     6901 tttctatgta atagttgctt gctccggctg caatagtgag caaataatct ttgttatcct
     6961 tcaattcttg agcatccagc ttttcgcgca tttccttaag aagcaaagtg aaattttgtt
     7021 tgtcttcagg tctttttgaa ttagttttaa ggcctcctcc gaccggatat tcccagtcca
     7081 aatccacacc gtcaagacca tactttgaaa taaactcaac acagctgtcc gtaaatttat
     7141 tcctgttttc gtctgtggcc gccatgtccg aaaatctccc cgaccaattc catccgccta
     7201 ctgaaattaa tacttttaaa tcagggttaa ttttcttaag atcccgaaac ttttcaaaat
     7261 tttcggggtc cttatccgga tatcccattt ctattctgta atccggacct atatttgcaa
     7321 acgcataatt tatatgggtt aatttgctta tatctacact ttccggagta tatcccgaat
     7381 aagacttcca tgcaggataa taccctataa ttatttgttg agctttttca gtattttcat
     7441 ttattgactt tgttttttca cttccttcgg ttaaaaaaga tgcagcttgt cctatgtaaa
     7501 acaaaagaat taaggttaat gatatgatta aatactttaa tcttgtcctc ttcatatatc
     7561 ctgtatgatg tatagacaat atacatcata ctcctcctat aaaacgtttt tttaattata
     7621 acataataat attgttataa atcttgaaat attaggcaat tccttatact cttagcttat
     7681 tttccattat aagtaaccaa tcgtgaagta attttgtttt gccttattgg caagtatatg
     7741 ttttgcttgt acaaagcaaa attatcagtt aaaataatac aataataatt actaaggaca
     7801 aaagaatgag aaacaatatt attttgacag aaggaaaaat acgaagtgca ttagtaaagt
     7861 tagcacttcc cataatgggc acttcattta taaatatggc atacaacttg acagacatta
     7921 tgtggcttgg caggctcagt accgatgccg ttgcagccgc cggtgctgca ggctattttt
     7981 tatggtttgg ctcttcattg gtgatgattg cacaggtggg agttggagtt atggttgccc
     8041 aattatttgg gaagggagat tcggaaggtg taaaaaacta tatcaacaac ggacttcagc
     8101 taaatctttt catagctata ttatactctt tatttttgta tattttcaga caccaaataa
     8161 ttggtttttt taatattgaa aatgaaaatg taagtaaaat ggcagttgag tatttgacaa
     8221 ttatttcagc aggaatcaca tttcactttt taaatcctgt tttctcagca attttaaaca
     8281 gcaccggaaa cagtattact ccttttaaaa ttaatacagt gggtcttatt gcaaatattg
     8341 ttattgaccc tttgttgata ttcggtttcg gtcccttgcc ggcattaggt gttaaaggtg
     8401 ctgccttagc tacaataatg gctcaattct tcgtgacgat gattttctta attataggga
     8461 aaagatatga tactatttat tcacatgcaa aattatttaa gaaaccaaga ctacccatta
     8521 ttcaaaatat agtcaaaata ggatttcctc cctttactca gatgggtatc catgcgggca
     8581 tagcaataat tctaaccaga atactggcag ttttcggacc cgttgcaatt gccgttcaaa
     8641 gtataggcgc tcaaatcgag tccattacat ggatgacaac tgaaggtttt tctgcagcta
     8701 tatctgcatt tgtagggcaa aattacggtg ccggaaaaat aaaacgagtc aaagacggat
     8761 atgtacaggg gattcaaatt gtagggagta tcggaatatt ttcaaccttg ctattgattt
     8821 ttggtgctga acaactattt tcattattta ctcccaatga cccgattgca ataaaggaag
     8881 gaataattta tttgcatata ttaggttttt ctcaattcct catggcaatt gaaattggta
     8941 ctaccggtgc atttaacgga ttaggaaaaa caattttccc cacgataaac ggaattatac
     9001 tgaatgtact tagaataccg gccgcattga tactaagcag gtcattagga ttgccgggtg
     9061 tttggtgggc aatagctata tcttcaaatt taaagggatt aatattattt attttatttt
     9121 atagatactc gaataatttc gagcgaacct atatttagta tttttttcat ctgagtgttc
     9181 aaaatttcaa ttaatgtgtg aattaaaaaa ttaatttatt ccattttaga cacattaatt
     9241 agaatttcga gcatatatga cctgtagaag tatcttttat atcaagataa ccattaattg
     9301 aatttttgag ctattcaatt tacaatttca tatatgcttt gattttttct tctgccgatg
     9361 aacttcttat ttattaacca acatagcttt ttctcatata ataactaaat agaatggata
     9421 ttattaggag gaaagaatgg ctatcagacc acctatttta agagcaggag ataccgtagg
     9481 cgtagttacc ttgggtagtc ctcgtgatgc cgaatctgtt gactctagaa ttcaaacatt
     9541 agaatctatg ggttttaatg ttgtagtagg cgaatttgta tattccgatg caggtattgt
     9601 tgctgcttcg cccatggaac gtgctgcgga tttaatgaat atgtttgtga atcccgaagt
     9661 tagagctatt atacctacaa gaggtggtac gggagtttcg gatatcattc cttatcttga
     9721 ttttgatgta ataaggcgca accctaagat tataaccgga tacagcgata taacaatttt
     9781 attgaatgtt ttataccaat tgtcagactt aattaccttt cacagcttat tgttaataga
     9841 ttttagaccc actacccctg catataattt tgaccaattt tttactgcca catcaacaat
     9901 aactgcacca agacctttgg agaatccccc cggtattccg ctgataagca gaattgaggg
     9961 aaatgtaaca ggtaccatag taggaggcaa tctaacgtct tttgttgata cacttggaac
    10021 accctttgaa attgatacta ccggaaggat tttatttatt gaagaaactc acgaagcagc
    10081 aagccgtgtt tacagaatga ttgctcattt gattcatgca ggtaaattca gagattgcgc
    10141 cggagttata atgggagaat gtgcaggttg ttatgcaaat tacggaaaaa cctatgaaga
    10201 cataataaat gaagtaattg ttcccttagg caaacctctt atgaccaatt tagcttcagg
    10261 gcacggctat tataaaatgg ccatacccat aggggcagtt actaatctta atacttataa
    10321 taatacattg acagtattgg aacctacagt gagcctgtag gttcttgcag gttattctat
    10381 ataatcgatt ggctgttttg gaagaaatcc tcccaagggt cctttttttc aatccttaaa
    10441 agagcatcct ccatattgat gccatccggc gcaaccgtat aaagctcttc ccaaaatata
    10501 ggcatagaca ccttagctcc ttttcttgcc ctcagtgaat aaggggcaat acttgtagct
    10561 ccccttgcat tccgaatcca gtcaataaat attttgcctt ttcttttagc ttttcttaca
    10621 ttgcttgtat atctttccgg ccacttattt tccattacct ctgcaacctg tcttgcaaaa
    10681 tcatgaaatt tatcccaatc ggcggtaggt tttaacggaa ttaccacatg atatccctta
    10741 ccaccgctta tcttaagata tgattttaaa tttagttcat caagtatact tctaatgtca
    10801 ataacccctt ggcgaacttt gtctaaatcc attccttcat ccgggtccaa atcaaatacc
    10861 atcatgtccg gcatttcaag attatcaaca ctgcttcccc aaatatgaaa ttccaaagta
    10921 cccatctggg cttcagatat taaccctgtt gcattctcta tataaaagta ttcacctgtt
    10981 tcatcatcgc ctgttgaaac gggcattgta ataattcctt tgcttccggg accggggtgc
    11041 tttttataaa agcacgcttg ggatactccc ttggggcaac gtacaatact taaaattcta
    11101 tgcctcacat aaggaagcat tttatctgca acctttgaat agtaacgaat aacttcttcc
    11161 tttgttatat cgagttcatt gtatatgatt ttgtcaggac ttgaaatttt aactccttca
    11221 ataataagat ctttttccat gtatcttccc tcttcatcat ttgattgggg cagaatcccc
    11281 tctgcctttt cccttttaat atccttagtg tccttatctt cccttaatcc tttgtagctt
    11341 gcttgcctta gcagattatc cttggtccat tctgcaaact ttatttctgc agccaatttg
    11401 ggctcaagcc aggtaatctt ttcattaggc tttgtctttg gcacctcctt gaaaggaggc
    11461 ttcgagcact ttaaaccctc aaatttactc tcaagaattt tcatatcatt ttcactgatt
    11521 cccgtgcctg cacgtcctgc atatattaaa tcctcaccct cgtaaactcc gagaagaagg
    11581 gagcttactc cgctgcttct tttatctgaa agtgtatatc ctccgataac gaactcttgt
    11641 cttttatcgc atttaatttt tatccagtca tcattccttt ttccgctgta aatggaatca
    11701 ggttttttcc ccactattcc ttccattcca gcttcacaag ctgcagcaaa gctttccttt
    11761 ccgtatcctt tcacatggcg gctgtagtaa agattttttg gcgcatcctg caataaactt
    11821 tcaagctttt cttttcttag aattaaagat tgtcctcgaa tatcctctcc gtcaatggcg
    11881 agcaaatcaa agacaatata agtaagattt tgagagttct gcgtttttaa atagttttgt
    11941 aaagcttgaa aatccgtctt gcccgattca tccgtaataa ctatttcacc gtctaatacc
    12001 attgctcttc ccttagccaa gtctataagc gaagaggcta tcgtaaaaaa ccgtttggta
    12061 atatcattgc cctttcttgt tataagacgg acactgttgc cttccacaaa tgccaaaatt
    12121 ctatagccgt catatttcat ttcataaagc caatcacctt ccgggattgc cttaactaat
    12181 ttggcaagct gaacatcagc tttactgaaa ggatttttga tgaacttttc atcttgacct
    12241 ccttcaattt cagccattgt tcgtccggtc ctgatactgg tggtaaattc ggacattcct
    12301 tcttcatcct tagaatactc atccttttct tttaataaaa gccagttatc ctgtgtttca
    12361 cctgccttac cttttaacct tatcaaagcc cacctgcctc taagccggct tcctttcaga
    12421 ctgaatttga gcataccttc actgagacct tcatccacat tcatatgagg ctcccagtat
    12481 ccttcatccc aaatcattac aaccccgccg ccatattccc ccttaggaat ggtaccttca
    12541 aaattcctgt attccaaagg gtggtcctct acctgtacgg caagcctttt atccttagta
    12601 ttgtaagaag gccccttggg tactgcccag cttagaagaa caccattcca ttccaaacgc
    12661 aaatcatagt ggtctctgct ggctaaatga tgctggacaa caaatcttaa actttcttct
    12721 gagctttgtg tctttccttc cggctcaaaa gttttttcaa aatttctttt ttgcttatat
    12781 tcatttaatc ctacagccat attatgccgt ttccttattc ttagctttct ctacactggc
    12841 tttcaaagcc tccatcaggt cgattacctt gcctgcactt tcaggctcgg cagcaacaac
    12901 ctctcttcct gaaatttttg cttcaataag ctcacgaagc ttcacttggt attcatcctt
    12961 gtatttggaa gggtcaaaag gagtatccat tgaatttata agaagctttg ccatatcgag
    13021 ctcctgcttg gatacttcag gcttggtata ttgtttttga atttctttga cttcatcggc
    13081 aaaaaacatg gtagaaatca atataccttc ctctcgagga ataatagcca tcaatgtgtc
    13141 cttggtgccc atgacagtct ttcctatggc tattttttgc tcggccatca gtgcagagcg
    13201 caacagttca aaagccttct ccccgcccgg ctccggcact gcctgatagg tcttttcata
    13261 ataaacgggt gaaatttggt tcaattctgc aaaatgaaga atttgaattg atttatcttt
    13321 ctccgtcttt attttttcaa tttcctcatc tgtgattaca acatacttgt ccttatcata
    13381 ttggtatcct tttacaattt cattagaagt tatttctttg ccgcaatgtg cacatgtttt
    13441 cttgtatctt attcggctgt tgtcttccgc atgcagctga ttaaaatgaa tatcattgtc
    13501 ttgcgttgcc gtatacattg caattggaat agcaaccatg ccaaaagtta taactgattt
    13561 acgtgatatc acataatcac ctccaaggtt attgttgcca attatatttt atgtattcga
    13621 catactgaca agtattggct atcaatcgta tataaaaaag acaaggaagc aaaacaaacg
    13681 tcccgtgtct tttgtaatta ttttcttaat atttgtgcct tgaaggttgt aagtttcgta
    13741 ttttcgttaa ttccttctat gtataccata ttatcttttg catcttctgt atcggtataa
    13801 agctgtatga tttcacccgg caatgcttca tgtatataat ctatatcgat tgctttgaca
    13861 gagtattttt tgtgattttc caagctgaaa cagtccatta tataatcgat gtattttgca
    13921 ttgttcaaat gctcgtttat atcaatgtca ctgtaaccga ctgttttttt ataacccaaa
    13981 tttagtttgc ctcttggttt tattttgttc agtgtacagt cgatagctct ttctctgtca
    14041 acctttggaa attcttcttc tattatactg cttcttctca gtttcctagt aatgaaatct
    14101 attacaaccc attggctaaa agccctgact ataatatttc catccttgtc ataaactaaa
    14161 aaattcctgt taaactgaac tttttccggc ggctccggcc aagtttcaat tgttatttct
    14221 tccttgaaca taggatatct tattacttca ccctttacac gatttaatac ccatatgatg
    14281 ccgtactttt tttgtaatac atctcttccc acaccgatat tataggcatg ctcggttgcc
    14341 atgtcctgca ggtataaaaa taacgagctt aacttaagct gccttctaaa atctgtttca
    14401 ctcggctgta ctatgtattt ctttctaaaa attgcttcca tcttacacct ctatcatact
    14461 caggatgatt agaacagact ttttaactca gcgacgaaat ttttggcatt attgatttgt
    14521 cttttaacat catcgtagga tgtgcctcca tagctgtttc tgttctttat acagttttct
    14581 acggtaaatt gactcaagtc ttcaatttca acgggaaagt ctaactcctt caattcttct
    14641 ttgtttatat cctgaaaagt ctttgatttg agctcgcaat atttaactaa cttacccacg
    14701 atttcgtgag cttccctaaa tggaatatta tgttttacca agaattccgc tatatccgtt
    14761 gcttctataa atcctttttt taaatgcgct cttaatacat cctcattaaa agttatattt
    14821 tcaagcattc tggcaaatat aaacactgtt ctgtccaatg tatgtatact gtcaaagagt
    14881 gcttctttat cttcctgata atccttgttt aatgttaaaa aggtaccttt taaaacggtc
    14941 aaaagtgtta tgagattccc tatggctcta cctgatttgc ctcgagccaa ttcggcaata
    15001 tccgggtttt tcttctgagg cataatactg ctacccgtac aaaagctgtc gtcaatagtg
    15061 atgaaattaa attcttgaga attaaaaaca ataatttctt ctgcaaaacc gcttaaatga
    15121 gtcatacata gagcagcatt aaataaatat tctgcaatgt tatctcttga agcaactgta
    15181 tccaatgcat tttccgtggg ttccgaaaaa cccattaatt ctgttgtcgc aaaacggtct
    15241 atgggcaatg tggttcccgc caatgcacac gatcctaagg gatttttgtc cagacgtttt
    15301 aataattctc ggaatctctc aacatttctt cgcaagcctt gaaaatgtgc catatagtag
    15361 aatccgaccg ttatcggctg agcgtgctgt aaatgagtaa aaccgggcat tattttatgt
    15421 gattcttctt ctgccttatt tatcaggaca tcaagtaaat ctattaaatg tttgattgaa
    15481 ttctttactg catcccgcaa aaacagcgtt tcatccaatg cattctggtc gtttctgctt
    15541 ctcgctgtgt gaagatattt tcctgcttct ccgacttcct tggtgagctc tttttcaata
    15601 atcatcatta tatcttcatc gagtatatta aattcaactt cattattttc aattttcttt
    15661 tctacaattg ataatcccct caatatttct tcgcatattt cattgcttat gatttgctgt
    15721 tttgcaagca tttttacatg agctttgctg cccgtaatac tgtgtttata caaaatatta
    15781 tcaaagctta tagattcatt gtaatcagcg gtatagtctt ccatactctt ggaaaaacga
    15841 ccgccccata aatttttcat acttttcatc ctttcagtta aaaatctccg gctgtgaaat
    15901 aatcgctcgg tactgttccg ggtcagtatc cccttccgta ctgaaacata ttattacaga
    15961 gttttcattt aattttaact tttcttttaa tggtttcaac tcttcttttt caagaaacag
    16021 ggttaacagt cctaacccca ctgcacctga ttctcctgat atgactttct tatcattacc
    16081 tgaaggagct gccaatattc tcattccttt agctgccaca taatcaggac aagacacgta
    16141 gacttttgca aaatctctta aaataggcca ggtaaccgta ttaggctctc cgcaagctaa
    16201 tcccgccatt atggtattaa ggtctcccga aacggaatga ggttttccgt cgtccgccaa
    16261 ggcagactta taaatacatg cggcattttc cggctccgca attacagtta cgggcggctc
    16321 atccttataa atatttgtaa agtatcccaa tatacctccc gcaaaggagc ccactcccgc
    16381 ctgtaaaaag acatgcgtcg gcttcatgct tccatccgac ttcatctggt ctatggcttc
    16441 ggctgccatt gtagtatagc cctgagcaat ccagttggga actttttcat atccttccca
    16501 agctgtatcc tgaactaata atccacctat ttcatcagct ttcttcttag ccatccttac
    16561 ggtatcgtca taatttacat ccgtaataaa agcttccgca ccagtatcct taatatgctg
    16621 caatctcctc agggaagacc cctttggcat atatacaatt gagttgcatc ccaattgagc
    16681 tgccgcccaa gctaccgctc ttccatgatt gccgtctgtt gcggtgacaa agaccatgtc
    16741 cttgattttt tctttcacca caggcagctt aaagtagtta taatctattg aatctatatc
    16801 cacacctaat ttctcgcaca acagtcttgc tatggcatat gttgcaccca agcctttaaa
    16861 tgcattaagt ccaaatcttt ttgattcatc ctttacatat atcttggaaa cacctaactt
    16921 ttcagctaaa cattccaagc ttaccaaagg cgtaatttca tagccgtcga ttgtagcatg
    16981 gaacttcctt actcctttta tttcttcttc acttaaaaag taaggcctca cacctttact
    17041 tttattattt attactgctt tgattccgca tgcttcatat atttccatta cataccccca
    17101 taattagtag tagaacgcta acattactat ataataagtg tatattaagg tcaatatttt
    17161 cctgccgaaa aaatattgga taaccttttg gttatcccaa atattattat gggaagtttt
    17221 tatattccct ataaatactt tatattaatt taataatgct atcttttcaa ttctatcgaa
    17281 tgcttctttc agtgtttcaa catcaactgt gcaggcaatg cgaatataac cttctccgca
    17341 agctccaaag gcatcccccg gcagcatcaa tacatgagct tctttcaaaa ttaaatctgc
    17401 cgcttcaacg cttgacagtc ctgttttttt aatattcata aacaagtaga agcttccctt
    17461 cggcggataa ataacagaaa gttttggaat cttatttatt cgctcagctg cataaaacat
    17521 tctcttctta tattcttcaa tcataggcgg ttgaattata tggcgatttt ttaaagcata
    17581 gattgcagcc ctttgcgaaa tcgacggtgc tgtgaaaact acattttcgt taatctgctg
    17641 gataactttt attatatagt ccggtgcaat aatatttcct attctccaac cggtcatagt
    17701 aaaatttttt gaaaaagagt taattataat tgttctgtct ttcatgcccg gaagacttgc
    17761 aaacggcaca aatggatatt gataactgaa agatgtataa atatcatctg aaactacaat
    17821 caagtcgtat ttttcagcga tttgtgcaat tttttccata acttcaacag taagacaaga
    17881 gccggtcgga ttgcttggtg agttaataac caatgccttt gtcttttctg taattaagca
    17941 ttcaagtcta tctacattta tttgaaagtc ttcttcttca taagtaggca gttcaacggg
    18001 tactcccctt gctaattcca cctgctgggg atatggcgta aagcatggtg cctgcagtat
    18061 tatttcatct ccatcatcaa caatagcttc taaggctaag tacattccaa gacatcccga
    18121 tgcacaaaca aagacttcct catctttaat atccatatca tattcttctt tgtaataatt
    18181 aattatttct tgccgcaatt cagggtcccc gcgaaagtcg gtatattttg tatgtcctgc
    18241 cttagcatca gcataagaat tatctattat aatatcgtgt gtaattaaat ccgggtcacc
    18301 cagacttaaa tttataatat cttcaaaaga cttcgctatc acatcagact gacccatggc
    18361 tgtactttta tctttccagt acctctttgc aataaactta tgtttcatta ctatcctcca
    18421 tttactatgc ctcttatttt aactgattgg cagatttgtt attcatctac acttttaccc
    18481 atattttaac atagtattca gcatatataa acattcttat tataggtaac tcattaaaac
    18541 tcttttttaa ttagttctgt aaaacctgaa ataactgctt cagtcagatg tctcccaatt
    18601 tccatagtag caccgtcagg atttgcatat gagccggatg aaggttgaac acgcttattc
    18661 caaggtaaaa tgcgataagg caatttctcg tactcacacg aatctacttt tatatcccta
    18721 accaactcag ggtataaata tttcatcagt gctgtctcgg ttaaagcagc atgttccgct
    18781 tcccaaccag gccatttatc cccaaatata ttatctatca tttcatcaga tataaattcc
    18841 caccaattag caattactat tttaggaatt tcactatttg tattttttga attttcataa
    18901 gctgtaagag ccagctcaat tccttccatc agaaaaggta tgctttcaag atgcccgttt
    18961 attaatataa tcttattaaa cccctgtctc aagaattccg aaatcaggtc taaacccaaa
    19021 ttaatcagtg tactcccgtt caaattgata gttcctaaat aattactgtt tagttttgca
    19081 taagtatatg atatagaagg agctattatt atttttttat ctatagcttt attatacaat
    19141 tttttagcaa aaccttcagc tataaatgta tccgtaccaa tgggcaaatg cggtccatgc
    19201 tgttcaaccg agcctgtcgg tatgataatg atgccatcac gaagattggt taattctgtc
    19261 catgttaatt catcccatct aaaaagagtc attgcttttt ccctcctgtc ttaatacggc
    19321 tattttaccg ttactcttat ctaaataaat gattctgtca tccccgtcca tagatgaaag
    19381 cctgtaaaag ggcttaaaaa taatactttt gttttgtatg ttaattgaag gtctgacaag
    19441 aaacgaccgt gctttagata gtacaaaagg cagcagagaa tctttcactt ttgccaatcc
    19501 ttccgcatcc atttttgcgg ttacaggcag taattgttca ggctttactt ctgcgtgata
    19561 tgtctctatt tgtttacaaa taaaacttat atcaccggtt aatccatcca cggctacctt
    19621 catactcatc ctttttactt tcgaaaaaag taaatgcatg caaacttcaa cctctaacaa
    19681 ccataacgga taaaatatct cttggcagtc ataatcttta aaatcagctg cttcattttt
    19741 tattgaattg tattctgcat tagtttgtaa gcatactata tccatagtta tcactgccta
    19801 attagttggt atcggctcgt tcaaagcatt tatgttcaat gattgtttta aaacattgtt
    19861 tttagtcata agcttttctg cacgcaaagc ttcagctttt aattcctccg aaacatttga
    19921 tgactttaca aaagattccg gaagcggtcc gtaagaagtt ccaatgtcaa aaagctcttc
    19981 catctcagga cctggttttt gtgtaagcag acttccaata ataaatgcta atatagcaac
    20041 aattaatcca ttcatgaagg gactcatacc aaacaactct attccggcag ctgtaagcca
    20101 tacatatacc gtcaatgaca aaataacaca agcaattgca ccggttttcg tactgcgctt
    20161 ccaataaacc gagaagatat tgggaatacc aaaagcaaga gccatgcctc cgaatactgt
    20221 cattactatg gtaaagactc ctgccggagg atttattgca gcaacaagtg aaagaattac
    20281 aatgattgcc gtaattatac gagcaatacg gattcttttc tcctgaggcg tatttggaag
    20341 taaaaatcta tttaatatat catgagcaat ggttgaaact ataagcatca agaatccatc
    20401 tgctgtagac ataatagctg caacagctgc agaaacaagg ataccggctc caaccggcgg
    20461 aagcacttca aaccaaagca aaacacctga tgtctcaggg ttttccaaac cgggtcccaa
    20521 taaaatcaat ccggcagctc ccaccatgta atatgcaaaa gaaaagagtg caacaaatat
    20581 tgcagttata agcattgctt tccttgccgt atcagggttt ttaagggata aatggcgtac
    20641 ggttacatga ggcattccaa atgaccccat tataataagt attataggag aagctgctgc
    20701 aataaggccg aaatatgagc cgtcaggtcc ccaaggtgta accaaaccgg ggtttattaa
    20761 agccaactgg tcatttaatg cgttaaaccc gcctacttta gaaattgcac tccaaaatag
    20821 aatatgagta ccgataaaca tcaccactcc ctgcataaaa tcagtataaa cagtggccag
    20881 atatcctccc attattgtat aagcaagtac cactgcaccg cctataagca ttccaactaa
    20941 ataatctgtt ccaaaagtta cactcagcaa tattccggat gcctgccatt gtgctacaag
    21001 gtgcagactt acagtagtaa taattataat agaagtcaac aatcttaatc ccgggctttt
    21061 gaaacgcttt tcgtaatatt caggcaaggt aatagctccc agcaactccg aaaacttcct
    21121 aagccttctt ccaagtaatc caaatgatag tgcgtttccg ggagcatcac cggttgacca
    21181 ccacatggta gaccatcctc ccgtatagga catcccggga gcagcagtat aagtataacc
    21241 gctggcataa gatgtaataa gtgcaaagga tgaaacccat gctcctatgg accttcctcc
    21301 aataataaaa tcatcataag tcttgattcg tcgcattgca tacatgccta ttccaagcat
    21361 tattaacaga taagatacaa atggtattat tacatttaat ttcatttttt tgcctccttt
    21421 aatttagctt tatcttattc ttttttttct tcacatgctt ccttttcttt ctttcccaaa
    21481 taccaataca aatagactgc cagagcaact actaacgcct gaacaaggaa aaaaatccat
    21541 gttcctgatg accaaaaaag ttttgaacct tccatctttt cacctccttc tttaaataaa
    21601 cttgcggctt tgtccacgat tactacacta aatttgaaat atatatctga aaataattac
    21661 tatctataaa cctatatgtg taaagctccg gaattattat caatatttat gcatgccttt
    21721 catccccata tgaagaatct tggtttctaa agggcaaaaa aaaactgccc ataaagaggc
    21781 agtgtttaca ccgcggtacc actcttttat gaagcagctg cttctctctt tcataataac
    21841 ggattttaac cgtattctca tactgctact tctgagaatc ttttcatgga ctcttttcaa
    21901 ttaaactaca ctaccgaact cacactctgc tccggctcgc tgaagcttta tttaatctac
    21961 tcttccaatc attaaattat acatagttta ttatttttat aaattataag ttggtttatt
    22021 tataatgtca acatattttt tttattattt gtttttttat ataggtaaaa aaataataag
    22081 gttattttca attatatcat tgatctgcat attggtagaa ttattctaaa tcaatcaact
    22141 tattgttaat atcaagaatt gatgttttaa ttgtaccgcc taaattatcc tgtaccagtt
    22201 ttgctaaatc atttatggca ttttttaatt cttccgttct tttttcatca accgattcaa
    22261 taaccaaaaa agcatttttt gtactttctg taagcatggt tcttactgat tctctccaat
    22321 tccagcacct gcaaattgcc ccttggtcat ctttataaac tatttcacct tcgtagggcg
    22381 gctcgctttt atccgagccg tatgtaataa aactttcgtc tccgactgcc tttgtcaatc
    22441 tgttatctcc cgcaaatgca tcaatatcct caccgccgca gggcaatgca tatctcaatg
    22501 aaatagaatt gtaaatatca actaatggat ttatagtacc tatatgattc ccgtttttta
    22561 ctctcttcaa caaggcctca atagagcatc ttgcaccttt ctttgttttg aatttttgaa
    22621 atgcctccct ccataccttt ataacatcgt tttgtataaa ctcaggattg tccaggtatt
    22681 ttaatgcttc atcctcggaa tctctaagca tatccaaata tttatccact tcttttattt
    22741 tgttgtctat attatgacat ataacaactc caaattttgc atcagggaac aattcccaaa
    22801 aatcatcctc gattataaat ttcttcattt gatacctcc
//
LOCUS       DAOPUE010000023        22628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2283_length_22628_cov_10.386701, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000023 DAOPUE010000000
VERSION     DAOPUE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2283_length_22628_cov_10.386701"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..414
                     /locus_tag="PKH42_03585"
     CDS             187..414
                     /locus_tag="PKH42_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOA19545.1"
                     /translation="MATNNSGSNNIVVPEAKQALNQMKTEIANELGLTNYEQTDKGNL
                     TARQNGYVGGYMTKRLVEMAQRQMSGGGQFK"
     gene            complement(525..1550)
                     /locus_tag="PKH42_03590"
     CDS             complement(525..1550)
                     /locus_tag="PKH42_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOA19546.1"
                     /translation="MNISEYKGVFVFAEQRDRTVQKVAYELIGKAKELAKDLNTTVTA
                     VLLGYDMIEEAKKLCYAGADKVIYADNELLDEYMTEPYTFTLHKIIREKKPEIVLYGA
                     SAIGRDVAPRVSARLKTGLTADCTGLAITEDDTNKGQLNLAMTRPAFGGNLMATIIIG
                     KHRPQMATVRPGVMQKAKYSENNPVNIEQFMIDIPADKKNVEILDITKIIQQRMNIED
                     AKVLVSGGRGMHGPENYQMLEELAGLLGGTISASRAAVDAGWVPKDRQVGQTGKTVRP
                     NLYIACGISGAIQHLAGMEESDFIIAINKDETAPIFEIADVGIVGDLFKIVPLLIEEL
                     KKSKQMA"
     assembly_gap    1601..1700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1701..2212)
                     /locus_tag="PKH42_03595"
     CDS             complement(<1701..2212)
                     /locus_tag="PKH42_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005953297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOA19547.1"
                     /translation="MNIVVCIKQVPDTTEVRIDPKTNTLIREGVPSIINPDDKAGLEA
                     ALRLKDEYNAHVTVLTMGIPNAEDALREALAMGADEAILITDRAFGGADTLATSTTLA
                     AAIKNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLVNISYAEDIKIDGDKIIVKRQYE
                     DRYHLLKTKLP"
     gene            complement(2226..3365)
                     /locus_tag="PKH42_03600"
     CDS             complement(2226..3365)
                     /locus_tag="PKH42_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOA19548.1"
                     /translation="MNFQLSKEHEMARQLFRNFAVEEVEPLAQEIDEEERFPVETVEK
                     MSKYGFMGIPFPKEYGGQGCDYLTYAMAVEELSKVCATTGVIVSAHTSLCGGPLYEFG
                     TEEQKKKYLTKVASGEWVGAFGLTEPGAGTDAAGQQTKAVLDGDNYILNGTKIFITNG
                     SYADCYIIFAMTDKSQGTKGISAFIVEKNFPGFSIGKKELKMGIRGSATCELVFEDCI
                     VPKENLLGKEGQGFKIAMKTLDSGRIGIAAQALGIAQGAIDESAKYVKERKQFGRAIA
                     KFQNTQFRLADMQTKVDAARLLVYRAACAKDAGQAYSVYAAMAKLFASETAMEVTTKA
                     VQLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVMKMVVSANMLK"
     gene            complement(3463..4236)
                     /locus_tag="PKH42_03605"
     CDS             complement(3463..4236)
                     /locus_tag="PKH42_03605"
                     /EC_number="4.2.1.150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain-enoyl-CoA hydratase"
                     /protein_id="HOA19549.1"
                     /translation="MEFKNLLFEKNEGICTIKINSPQTLNALNEEVLEELDSAFDLAG
                     KDEEVSAIILTGEGKAFVAGADIAYMNNLNVEEAKKFAEYGSKIFRKIELLEKVVIAA
                     VNGYALGGGCELCMACDIRIASEKAKFGQPEVGLGITPGFSGTQRLPRLVGLGRAKEL
                     ILTGGHIDAEEAYRIGLVNKVTNKDSLMEEAFTLAKKIISNSKVAMKYAKEAMNRGIE
                     TDIETGIAIEANLFSLCFADEDQKEGMTAFMEKRTAKFK"
     gene            complement(4440..5084)
                     /locus_tag="PKH42_03610"
     CDS             complement(4440..5084)
                     /locus_tag="PKH42_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOA19550.1"
                     /translation="MIDTVIFDFDGTLVNTNNLILNSFNHIYCKYHNRNCDEDYVLST
                     FGEPLELTLKRDFSDFNYEEVVGSYRQYQVDKFDKEVCLFDTVSETIKYLHDKNIKIG
                     IATSRLKSSTLHALKIFNLEKYFQVVITAEDVVKHKPDKEPLIKVIQELNSNPENTLY
                     VGDSKFDMECAINAKVTPVLVGWHKNSDELAEKYNIKYVLNKMWDLTEIIIMSS"
     gene            complement(5077..7377)
                     /locus_tag="PKH42_03615"
     CDS             complement(5077..7377)
                     /locus_tag="PKH42_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOA19551.1"
                     /translation="MIDINKILCTEFNLKPYQVENTVKLIDEGNTIPFIARYRKEQTG
                     SLDDVVIRDLFERLTYLRNLETRKEEVIRLIEEQGKLTDELKNEILNADVLQRVEDLY
                     RPYKQKKSTRASKAKEKGLEPLAEIILAQNVTEGDLDEIAGPFVNEEKGVNSVKEAYE
                     GACDIIAETVSDNADYRKHIREVYINDAILISEAADEEEKTVYEMYYKFEEPVNKIAN
                     HRVLAINRGEKEKKLKVKLNAPDEKIINYLISKEITNENAITKDFYLSAILDSYKRLI
                     APSIEREIRTMLTERAEEEAIKVFGKNTKNLLMVPPVKDIRILAIDPSFRTGCKLAVL
                     DETGKFLEQGVMYPNEPRNEVEESQKLMADLINKFDIDVIAIGNGTASRETEMVVSDM
                     LSKYTFSKKVSYTIVSEAGASVYSASKLAQEEYPDLDVTIRGAISIGRRLQDPLAELV
                     KIDPKSIGVGQYQHDVNQTKLGQELDGVVEDCVNSVGVDLNTASPALLQYVSGITKTI
                     SKNLVKYREENGKFVNRQELMKVKMLGDKAFEQCAGFLRISDGDNPLDKTAVHPESYE
                     IAGKLLKKLGYTIQDLRSGTIRDINERIMKIPVKEDKAITPAKNVRLKGLEALAQINF
                     KENKKSFNEKIDDRIKVLSEELNVGVPTLTDIIGELKKPGRDPREEMPKVVFRSDVKS
                     FEDMKVGMNLTGTVRNVVDFGAFVDIGVKQDGLVHLSEMSDKYIKNPMDVVQVGDTVN
                     VTIISIDKERQRIGLSMKKSLRSKND"
     gene            complement(7480..8590)
                     /gene="prfB"
                     /locus_tag="PKH42_03620"
     CDS             complement(join(7480..8514,8516..8590))
                     /gene="prfB"
                     /locus_tag="PKH42_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157593255.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOA19552.1"
                     /translation="MTDWYEITERIKKFNDILNEVGGSLDLEKLREENFTLESQMQEP
                     GFWNDQEKAQKVSQKLKHNTNRIEEFNKLKQQVEDIELFIELIKEEDDESHVPELLKN
                     MDEAEEQLESARIVALLSEEYDGNNAILSIHAGAGGTEAQDWADMLFRMYTRWIESKG
                     YSMEIYDIQTDTEAGIKSVTVFVEGQNSYGYLKSEKGVHRLVRISPFDSSNRRHTSFA
                     SVDVTPEIEDDEDIEINEDDIKIDTYRSSGAGGQHVNTTDSAIRITHIPTGIVVSCQN
                     QRSQHSNKETAMNMLKSKLLEIKRLENKEKIEDIQGKYNQIAWGSQIRSYVFHPYSMV
                     KDHRTDAETSNVQSVMDGNLDMFMNEYLKMKSRER"
     gene            complement(8724..11489)
                     /gene="secA"
                     /locus_tag="PKH42_03625"
     CDS             complement(8724..11489)
                     /gene="secA"
                     /locus_tag="PKH42_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOA19553.1"
                     /translation="MKNFFEKIFGSYSEKEIKRIQPIVDKVLSLEKTMETLTDSQLKG
                     KTAEFKQRLANGETKDDILPEAFAVVREAAWRVLGQKHYPVQIIGGIVLHQGRIAEMK
                     TGEGKTLVSTLPVYLNALEGNGVHVVTVNDYLARRDADWMGKVHNFLGLSVGCIVHGI
                     TKEERLSAYRSDITYGTNNEFGFDYLRDNMVIHKEDMVQRDLNFCIIDEVDSILIDEA
                     RTPLIISGEGEKSTDLYEMANSFVLNLKGKIQLPEEKKTKMDYIMGDVEEDDTVDYIV
                     DEKAKNVTLTARGIDKAEKYFGVENLADQENMELTHHINQALKAHNTMKKDIDYIVKD
                     GEVLIVDEFTGRIMYGRRYSNGLHQAIEAKERIEVRKESKTLATITFQNYFRMYKKLS
                     GMTGTAKTEENEFREIYGVDVIEIPTNKPVIREDLQDVVYKGEAGKFKAVINEIIERH
                     QTGQPILVGTISIEKSEILSKLLKKQGIKHEVLNAKQHDKEAEIVAQAGRLGSVTIAT
                     NMAGRGTDIVLGGNPEFIAKHKLKAKGFSDELIAQADGFNETDDAEILEARRVYKQLL
                     EESKNELRDEQKKVIEAGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSSRFYISLED
                     DLMRLFAGDKVKAIIETLKMPEDEPLEAGMLTRTIETAQARVEGRNFDIRKHVLQYDN
                     VMNKQREIIYSERRRVLLGEDLKEYISNMIDSLIDKAINMYTTDEKNNENWDLNGFVK
                     YIAETFSLLISFDGVKPEDIDKEDLRKRVRIAVDKHYARQEAEFGEETFREVERIVLL
                     RNVDTKWMDHIDAMDQLKQGIGLRAIGNEDPVRAYQVEGFDMFEEMTEAIQEDTVKML
                     MRVRPQEKLQRKQVARITSVSGADADSIGGRPQPLVKKEKKVGRNDPCPCGSGKKYKK
                     CCGANEK"
     gene            complement(11509..12729)
                     /locus_tag="PKH42_03630"
     CDS             complement(11509..12729)
                     /locus_tag="PKH42_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOA19554.1"
                     /translation="MKIAVIGGGASGLTAAIAAARNGAEVTICEKLNRVGKKILATGN
                     GRCNYTNMNLSKECYHSNNLNFVDEVMKFFNLDKTLVFFEDLGILPYVDESGKVYPNS
                     LQASSVLDVLRYELKRHKVKEVTDFNVTALRKSKDKFSIIGNDTITADRVILATGGKA
                     SPQLGSDGKGYELAKSLGHEIIEPFPALVQLKLSGKYFKRIAGIKFDGIVKAYAGDRL
                     IREEEGEILFTDYGISGPPILQVSRKVIEELNKKNKPFLNIDMFPGYSKLKLYDILQD
                     RFRRINYKTIEESMIGFINKKLIPVVFYEAGFEDLNKICGKLNKKEIYKIIEILKEWK
                     FEVIGHNSWQQAQSTAGGIKLSEVNPKTLESLKVKGLYFAGEILDVDGDCGGFNLQWA
                     WSSGYTAGYFSSLE"
     gene            complement(12733..14334)
                     /locus_tag="PKH42_03635"
     CDS             complement(12733..14334)
                     /locus_tag="PKH42_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19555.1"
                     /translation="MYRIQQIKLPIDHKEEELLIKAAEALRIKKEEIKEISIFRKSID
                     ARKKDEILFIYTLDVDTYKEVKISKRNNNISVAEPFIYDVQATGEKPLNNRPVVVGSG
                     PAGLFCALLLAEKGYRPIILERGKTVKERVKDVEKFWKEGILNYSSNVQFGEGGAGTF
                     SDGKLNTLIKDRSKRGKKVLEEFVEAGAPAEITYINKPHIGTDLLRNVLVNIRKKIIS
                     LGGTFRFEECLTDIIIKDNAVQRIVTESDIIETDILVLAIGHSARDTFEMLNNKLKLT
                     AKPFAIGVRVEHPQKMICEAQYGEMAGNPLLGPADYKFVHKSKNGRSVYTFCMCPGGV
                     VTASASEAGGVVTNGMSFHERDLENANAAVVVQINPEDFGGEENPMAGVEFQRYWERK
                     AFEAGGGNYRAPVQLFKDFKEKIVSSSFGEIYPTYRPGCTFANLWDCMPDYVCESLVE
                     GITAFGKFLTNYDRPDALLTGVETRTSSPLRILRDDEMQSNVRGIYPCGEGSGYAGGI
                     MSASIDGLKVAEQILKTYKTINDKE"
     gene            complement(14395..14811)
                     /locus_tag="PKH42_03640"
     CDS             complement(14395..14811)
                     /locus_tag="PKH42_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOA19556.1"
                     /translation="MDQTDLKIISILQNNGRISMKELGKIVSLSPPAVAERVKKLEET
                     DVIQRYTAVINNEKIGKPITVLINASIKPENQKDFLHFAENKEEIAKCHHVTGPHSMI
                     IKANLREMKHLEELVREIQVYGNTETYIIMSSPIDR"
     gene            15057..16400
                     /locus_tag="PKH42_03645"
     CDS             15057..16400
                     /locus_tag="PKH42_03645"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HOA19557.1"
                     /translation="MTKLKITETSLRDGHQSLIATRLSTPEMLPILQEMDKAGYHAME
                     VWGGATFDTCLRYLNEDPWERLRKIRANIKNTKLQMLSRGQNLLGYKNYPDDIVERFH
                     KNAISEGIDIVRVFDALNDIRNLQKTIDTVKSEGAHLQCAISYTISPVHTKEYYLKLI
                     KDFENAGADSVCIKDMSGILLPYTAYDLVKSIKEVTNLPIELHTHCTGGIGEMTVLKA
                     VEAGVDIVDTAISPLSSGTSQPATESLCLTLKDTERDPQLDLMKLNEIADHFKNVMKK
                     FEEEGTLNTKVLMTEPKTLLYQIPGGMLSNLLIQMKSLNAVDKFQAVLDEVPKVREDL
                     GFPPLVTPMSQIVGAQAVFNIVTGERYKVIPTEIRNYVRGLYGKPAAPISDEIRKLII
                     GDEEVITVRPADLLENSYEQMKEEIGGLAKSEQDVLSYALFPPVAKAFFEKRAGK"
     gene            complement(16574..17791)
                     /locus_tag="PKH42_03650"
     CDS             complement(16574..17791)
                     /locus_tag="PKH42_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOA19558.1"
                     /translation="MKKELNKETARIILKALEDAKKHMQYLQERREEKLFSEIEIPCN
                     LNDVICRLTKDEMSHICKNYKLQGLSSLKKSELASELIKIIPHKFEGIIRILDQNTYD
                     LIKSIVKNSGAIPCKDIKFSIIENLMRYAITFPGLKNGERILVMPVELMEKFNILDTE
                     ERQKVIHRNTEWIQLTQGMLYYYGVMDSWRLIKNVQKYVKENININQFAEVISLACNL
                     YENLDKIPSYGYKDARVFDSENIIEEHNKRPELDYYQFTKSQLLKAGNQNHKNTSPEM
                     NSFIDFLLESYDLKDDDIDEISTQLTNLINQDSEPTFIIKYLETWLEIPSLEYKQVLT
                     LKVIELSNNTRQWALKGYTPSELLQEENSNTSKAKIIKMTKSNKIGRNEPCPCGSGKK
                     YKKCCGMNKNVTE"
     gene            complement(17887..18636)
                     /locus_tag="PKH42_03655"
     CDS             complement(17887..18636)
                     /locus_tag="PKH42_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOA19559.1"
                     /translation="MPRVARKLSETGIYHVIFRGVNRFNIFEEDKDYEKILEIISDLK
                     EKISFDIYAYVLMNNHVHLIIKELELGEISLIMQRILTRYAGWFNRKYDRSGILIANR
                     FKSDPVETEDYLLTLARYIHQNPVKGGLTSKIDSYKWSSFKEYLGKSEICNTDFIMSI
                     IDRDSFIKFNFEINEEEYEISDKIQKFDDEFVKKRIKEILKGKEPTKLGEMPIDYRNR
                     IIKQLITTEKFSIRQIERATGISRGVISRCK"
     gene            complement(18752..20485)
                     /locus_tag="PKH42_03660"
     CDS             complement(18752..20485)
                     /locus_tag="PKH42_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19560.1"
                     /translation="MNNTAQDDKRALNIIWNASDDYSIEPQFKAYDKNGEADIYWNYI
                     IGAVHKYYDYTLLRGFFNYLKTDREHEFYEKLFWIGLEKSAFDKGKNERPVIVSLRKN
                     YARKVLAGETEVFDDNLLNVLTNAHFKRALGKAPGITGNALNILNDLEFGDASTEEII
                     LKMNQIIKKYFRFNYGQYEVNAQINKKRVQRQIQYCEEERLSDSDMETMKELYLDSAE
                     TSREVFYEEKKGKIKKSAKKVKIEKKDDSERQYIRKYFGASIFPDKKTYLIERKLCIG
                     NHKNTHLHFTRGEFDDEFSNDINALYFNKALKEQREINLEHFNSNYIKYKNSILKLTN
                     KIRNTMLAYFESYTGKSEAGRLNAPKIWRNVYLNDNRVFTKVYNNDIGNLSVDILLDA
                     SASQHERQETIAAEAYIIAESFTQCQIPVRVYSFSSLRNYTVINLYRDYDETDKNTKI
                     FNYKTTGCNRDGLAIRTALYMMEGSNYLNKILIVLSDCKPNDTQCIPDTGIKQEQTKY
                     AGATGIIDTAFEVKRGINNGNSILCVFTGEDEDIPTAKKIYGHNFARIYYPERFAEIV
                     GVLMQNQLKNL"
     gene            complement(20488..>21075)
                     /locus_tag="PKH42_03665"
     CDS             complement(20488..>21075)
                     /locus_tag="PKH42_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19561.1"
                     /translation="GQVVFRNGPIYECAKWGGFGVLDEINMAKNDSVAVLHATLDYRR
                     IIDVPGYDKITIDDASRFIATMNYGYAGTRELNEALTSRFMVINMPNISSKNLRKLLQ
                     REYPNLREEYVEQFAGLFKDLQLKSENAEISTKSVDLRGLLASINLMDKGLSPFKALQ
                     MGITNKSFDDFERTLVEDVIRLRIAENLGRNEIFR"
     assembly_gap    21076..21175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21177..22535)
                     /gene="radA"
                     /locus_tag="PKH42_03670"
     CDS             complement(21177..22535)
                     /gene="radA"
                     /locus_tag="PKH42_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOA19562.1"
                     /translation="MAKIKSKFVCQECGYETAKWLGKCPSCEEWNTFVEEFETKKSDS
                     KSQRGISKGIIEKINKITSTKKERISTGSVEMDRVLGGGIIKSSLILVGGDPGIGKST
                     LLLQVADHAAKQKLKVLYVSGEESGEQIKIRADRLGIGDGELYVLAETNIDIIIEHVD
                     KEEPDLLVLDSIQTIYSPDVPSAPGSVTQVREVTSMVMRITKTRNMASFIVGHVTKSG
                     AIAGPRVLEHMVDTVLYFEGERHFSYRILRAVKNRFGSTNEIGIFEMRDRGLIEVENP
                     SEVFLKGRPLDAYGTVVTAAMEGTRPVLIEIQALVTYSPVGFANRVTTGIDKNRASML
                     IAVLEKKAGLSIQSSDIFINVTGGLQIKEPACDLAILCALASSFKEIPVDFKTVLIGE
                     VGLTGEIRGVNSIEKRLLEAKKMGFKRAVIAEANAEISKEVKNMEIIPVNNIRQVMDI
                     IF"
BASE COUNT         5976 a         4739 c         3425 g         8288 t
ORIGIN      
        1 aataataagc atagaaagtc agttttgatt ttctaaaatt taatcaatgt aaaattttgc
       61 aacctgttac ataaaacagg ttaatggggc ccatgagtaa tttaccaatt taacataata
      121 ttacatgatt aaattgaacc taattgataa taataagaat acatcttaag agaaaggaga
      181 atgaaaatgg caactaacaa cagcggaagc aacaacatag tagttcctga agcaaaacaa
      241 gctcttaacc agatgaaaac tgagatagct aatgaattag gtttaacaaa ttatgagcag
      301 acagacaagg gtaaccttac tgcaagacaa aatggttatg taggcggata catgactaag
      361 agattggttg aaatggctca gagacaaatg agcggcggcg gacagtttaa atagtaaata
      421 aggaaagggg gacacacctc tactttgagg atatactccg gggtagagga ttgtccccgt
      481 acttataaaa ttggtggatt agaatatcca ccaatttttc tgatttaggc catctgctta
      541 ctttttttta attcttcaat aagtaaggga actattttaa ataaatctcc tactataccg
      601 acatctgcaa tttcaaatat aggtgctgtt tcatccttat ttattgcaat gatgaaatca
      661 gattcttcca tacctgccaa atgctgaata gctccggaaa ttccgcatgc gatataaaga
      721 tttggtctta ctgtctttcc ggtttgtcca acttgtctgt cctttggaac ccatcctgca
      781 tcaactgctg ctcttgatgc agaaattgtt cctccgagaa gtcctgccaa ttcttcaagc
      841 atttgataat tttcaggacc gtgcattcct cttccgcctg ataccaatac ttttgcatct
      901 tcaatattca ttctttgttg aattattttt gttatatcaa gtatttcaac attctttttg
      961 tctgcaggaa tatctatcat aaattgttca atatttacag gattgttttc ggagtattta
     1021 gctttctgca taactcccgg ccttacagtt gccatctgcg gcctgtgttt tcctattata
     1081 attgtagcca tcagatttcc gccaaatgcg ggtctagtca tggcaagatt taactgtcct
     1141 ttgtttgtat catcctctgt aattgccaga cctgtacagt ctgccgttaa accggttttc
     1201 aatctagcgg atactctggg agccacatct cttcctatag ccgatgcacc gtataaaact
     1261 atttcaggtt tcttttctct tattatcttg tgcagtgtaa aagtatatgg ctctgtcata
     1321 tattcatcca gcagttcatt gtctgcgtat attactttgt ctgcaccggc atagcataat
     1381 ttttttgctt cttcaatcat atcataacct aataatactg ccgtaaccgt tgtatttaaa
     1441 tcttttgcca attcttttgc cttgcctatc aattcatatg ctactttttg tactgttctg
     1501 tctctttgtt cagcaaaaac aaaaacacct ttatattcag aaatattcat ccggttctcc
     1561 tcctatatta taaatttttc tttcattttt tcaacaatca nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn ggtaattttg tttttaacag atggtatcta tcttcatatt
     1741 gcctttttac aataatttta tcaccgtcta ttttaatatc ttccgcatag cttatattta
     1801 ccaatcctaa atgttctgct atttgcggac caacctgtgc ggtatctcca tctattgcct
     1861 gacgtcccgt tatgattaaa tcataatcaa gattttttat ggcagctgca agagttgttg
     1921 aagttgccaa tgtatcagca cctccaaatg ctctgtctgt tatgagtatt gcttcatctg
     1981 cacccattgc caaagcttct cttagggcat cttctgcatt tggaataccc attgttaaga
     2041 ctgttacatg agcattgtat tcatctttca atctaagtgc tgcttcaaga cctgccttgt
     2101 cgtcagggtt tattatgctt ggtacgcctt ctctgattaa tgtgttagtt ttcgggtcaa
     2161 ttctaacttc tgtcgtatcg ggcacctgtt tgatacatac tactatattc actgtctttg
     2221 cctccctatt tcaacatatt tgcactgaca accattttca ttacttctga agtaccttca
     2281 taaatttcag ttatcttcgc atctctcatc attctttcaa caggataatc tctcgtatat
     2341 ccatatccgc cgtgtagctg aacagccttt gttgtaactt ccatcgctgt ttccgatgca
     2401 aacaatttag ccattgctgc ataaacacta taagcttgtc ccgcatcctt agcgcatgct
     2461 gctctgtaaa ccaaaagtct tgctgcatct actttagtct gcatatccgc taacctaaac
     2521 tgtgtatttt ggaattttgc tattgctctt ccaaattgct ttctttcttt tacatattta
     2581 gctgattcat ctatagcacc ttgagcaata ccaagcgcct gagcagcaat tcctattctt
     2641 cctgaatcaa gtgttttcat tgctatttta aatccctgtc cttcttttcc cagaagattt
     2701 tcctttggta ctatacagtc ttcaaaaact aattcacagg tagcagagcc tctgataccc
     2761 atttttaatt cttttttccc tattgaaaag ccgggaaagt ttttttctac tatgaaagca
     2821 gaaatacctt ttgtgccttg tgatttgtcg gtcattgcaa aaattatata gcaatccgca
     2881 taggaaccgt ttgttataaa aattttcgtg ccgttcaaaa tataattgtc accatcaagc
     2941 actgccttcg tctgctgacc ggctgcatct gtgcctgctc cgggctcagt aagcccaaat
     3001 gcacctaccc attctcctga cgcaacctta gttaaatact ttttcttttg ttcttctgtt
     3061 ccaaattcat atagaggacc gccacacagc gaagtgtgtg cagatactat aacgcctgta
     3121 gttgcacata cttttgacaa ttcttcaacc gccatggcat atgtcaaata atcgcagcct
     3181 tgtccgccgt actcctttgg aaacggtatg cccataaaac catacttgct catcttttca
     3241 acagtttcaa cgggaaatct ttcttcttca tcaatttctt gtgccaatgg ctcgacttct
     3301 tccacagcaa aatttctaaa cagctgtctt gccatttcgt gttccttgct taattgaaaa
     3361 ttcatgattt cactccctaa attttttatt tgttaatttt ttaacattta attacgcgct
     3421 aacgcattaa ttatatataa ttgcttaaaa tatatcaagt gcttatttaa atttagcagt
     3481 acgtttttcc ataaaagcag tcattccttc tttttggtct tcatcagcaa agcataggct
     3541 aaataaattc gcttcaattg caattcctgt ctctatatct gtttcaattc ccctgttcat
     3601 tgcttctttt gcatatttca ttgcaacttt ggaatttgaa ataatttttt ttgctaatgt
     3661 aaatgcttct tccataaggc tgtctttgtt agttacttta ttaacgagtc ctatcctgta
     3721 tgcttcctct gcatctatat gtccgcccgt aagtattaat tcctttgccc ttccaagtcc
     3781 cactaatctt ggaagcctct gagttcctga aaatcccgga gtaattccaa gaccgacttc
     3841 cggctgtcca aacttagctt tttctgatgc aattctaatg tcgcaagcca tgcagagttc
     3901 acatccgccc ccgagagcat acccgtttac agctgcaatt acgacttttt caagcaattc
     3961 tattttcctg aaaattttag agccgtattc cgcaaatttc tttgcctctt ctacattaag
     4021 attgttcata tatgctatgt ctgcacctgc tacaaaagct ttcccttctc ctgtaagtat
     4081 aattgcggaa acttcttcgt ccttacctgc taagtcaaaa gcagaatcaa gctcttctaa
     4141 tacttcctca tttaatgcat tcaaagtttg cggactgtta attttgatag tacaaattcc
     4201 ttcatttttt tcaaataata aatttttgaa ttccatatca ataccatcct ttctgtttgc
     4261 taattgtatt atattaatga attgctatca caatattcaa actattgcaa acacaatcaa
     4321 cattgtcatt tctatgaata gcctgcggag tgtcattgct atgattatgc tgcgtgtcat
     4381 tctgagagct ttagctcgaa gaatctcatc ttataaaacc ggagatcctt cgcttgcgct
     4441 caggatgaca taataattat ttcagtcaga tcccacattt tattgagtac gtatttaata
     4501 ttatattttt ctgccaattc atctgaattt ttatgccatc ctaccagcac cggagtaacc
     4561 tttgcattta tggcacattc catgtcaaat ttagaatcac ctacatataa agtattttcc
     4621 ggattgctgt ttaattcttg gataacctta atcaaaggct ctttatcagg cttgtgcttt
     4681 accacatctt cagcggtaat aacaacttga aaatatttct ccaaattaaa aatttttaag
     4741 gcatgcaagg tggatgattt aagtcttgat gttgcaattc cgatttttat atttttatcg
     4801 tgcaaatact ttatagtttc gcttaccgta tcaaataagc atacttcttt gtcaaatttg
     4861 tcaacctgat actgtctgta gcttcctaca acttcttcat aattgaagtc gctaaaatcc
     4921 ctctttaagg ttaattcaag aggctctcca aatgtgctta aaacataatc ttcatcacag
     4981 tttcggttat gatatttgca gtaaatatgg ttaaatgaat ttaatatcaa gttattggta
     5041 ttaactaagg ttccgtcaaa atcgaatata actgtatcaa tcatttttgc tccttagact
     5101 ttttttcata cttagaccga ttctttgtct ttctttgtct atgctaataa ttgttacatt
     5161 gacggtgtct cctacctgaa caacatccat ggggttttta atatatttgt cgctcatttc
     5221 ggaaagatgc accaaaccgt cctgctttac ccctatgtct acaaatgctc caaagtcaac
     5281 aacgttcctt actgttcccg taagattcat tccaaccttc atatcttcaa agctcttaac
     5341 atcgcttctg aatacaacct taggcatttc ttcgcggggg tctctgccgg gttttttaag
     5401 ctctcctata atgtctgtta atgtaggaac tcccacattt aattcttcag ataaaacttt
     5461 tattctgtca tcaatttttt cgttgaaaga ttttttgttt tccttaaagt ttatttgagc
     5521 caatgcttcc agtcctttta gccttacgtt tttagcaggt gttattgctt tatcttcttt
     5581 tacgggaatt ttcattattc tttcatttat atctcttatg gtaccgcttc ttaaatcttg
     5641 aattgtatat cctaattttt tcagaagttt tcctgcaatt tcatatgatt cgggatgtac
     5701 tgcagtcttg tcgagaggat tgtctccgtc ggaaattctt aaaaaacctg cacattgctc
     5761 aaaagcttta tcacctagca tttttacttt cataagctcc tgtctgttta cgaatttccc
     5821 gttttcctcc ctgtatttaa ccaaattttt tgaaattgtc tttgttattc ccgacacata
     5881 ttgtaataat gccggagaag ccgtattcaa atcaacacct acactgttaa cgcagtcttc
     5941 aacaacgccg tcaagctcct gtcccaattt tgtctgattc acatcatgct gatattgacc
     6001 tacaccgata cttttggggt ctattttaac aagttcagcc aatgggtctt gaagtcttct
     6061 tcctattgaa atagctcctc ttatggtaac gtccaggtcg gggtattcct cctgagccaa
     6121 ttttgatgcc gaataaacag aagctccagc ctcacttacg attgtatagg aaactttttt
     6181 agaaaatgta tatttagaca gcatatcaga tacgaccatt tctgtttctc ttgaagctgt
     6241 tccgtttcct attgcaataa catctatatc aaatttattg attaaatcgg ccatcagctt
     6301 ttgtgactct tctacttcat ttcgcggctc attggggtac ataactccct gctccaagaa
     6361 cttgcctgtt tcatccaaaa cagctaattt gcatcctgtt ctgaagctcg ggtcaatggc
     6421 aagaatcctt atatccttaa ccggaggaac catcaataaa ttcttggtat ttttgccgaa
     6481 tacctttatt gcttcttctt ctgctctttc agtaagcatg gttcttattt ccctttcaat
     6541 ggaaggggca ataagccttt tataactgtc caaaattgcg gacaggtaga agtctttagt
     6601 tattgcattt tcatttgtta tttcttttga tatcaaataa ttaattattt tttcatcggg
     6661 tgcatttaac ttaactttta atttcttttc cttttcgcct ctgttaatgg caagaaccct
     6721 atgatttgca attttattta caggctcttc aaatttataa tacatttcat agacggtttt
     6781 ttcttcttcg tcggctgctt ccgatatcag tattgcatcg tttatgtata cttctcttat
     6841 atgttttctg taatctgcat tatcggaaac agtttcagct attatgtcgc aggcaccttc
     6901 ataagcttct ttgacactgt ttacaccttt ttcttcattt acaaaaggtc ctgctatttc
     6961 atcaaggtct ccttcggtaa cattttgcgc caatataatt tctgccaaag gctcaagccc
     7021 tttctctttg gcctttgatg ctcgtgtgga ttttttctgc ttatatggtc tgtataagtc
     7081 ctcgactctt tgcagcacat ctgcatttaa tatttcgttt ttaagttcat cggtaagctt
     7141 tccctgctcc tcgattaacc tgattacttc ttcttttctg gtttctaaat ttcgtaagta
     7201 tgtaagtctt tcaaataaat ctcttataac cacatcgtcc aaggagcctg tctgctcctt
     7261 gcggtacctt gcaatgaagg gaattgtatt cccttcatct attaatttga ccgtattttc
     7321 aacctggtag ggttttaaat taaattccgt acacaatatt ttgttaatat ctatcattct
     7381 tctctcccct gtacaatggt gattttccat attttctttg acattatttt cgccgttccg
     7441 agccgcaagt taacctttct aacgctcagg atgacaattt tatcgttctc ttgatttcat
     7501 tttcaaatat tcattcataa acatatctaa attgccgtcc attaccgact gaacatttga
     7561 tgtttcggca tccgtgcgat ggtccttcac catgctgtaa gggtgaaata catatgagcg
     7621 gatttggctt ccccatgcaa tttgattgta ttttccttga atatcttcga ttttttcctt
     7681 gttttccagc ctctttattt ccaaaagctt tgattttagc atgttcattg cggtttcctt
     7741 gttgctgtgc tgagacctct ggttttggca ggaaaccact atgcctgtag gtatatgtgt
     7801 tatgcgtatt gcggagtctg tagtattaac gtgctgtcct cctgcaccgc tcgagcggta
     7861 agtatcaatt tttatatcat cttcattgat ctcaatatct tcatcatctt caatttcagg
     7921 tgttacatcc actgatgcaa aggatgtgtg cctcctgttt gaagagtcaa aaggtgatat
     7981 tctgacaagt ctgtgtacac ctttttcgga tttcaaatat ccgtaagagt tttgcccttc
     8041 aacaaaaacg gtgacactct taattcccgc ttctgtatcc gtttgtatat catatatttc
     8101 catactatag cccttggatt ctatccacct ggtgtacatt cgaaacagca tatccgccca
     8161 atcttgtgcc tcggtgcctc ctgctcccgc atgaatggat aaaattgcat tatttccatc
     8221 gtattcttcg cttaaaagag caactattct tgcactttcc aattgttctt cagcctcatc
     8281 catattcttc agaagctccg gcacatggct ttcgtcatct tcttctttta tcaattcgat
     8341 gaaaagctca atatcttcta cctgctgttt taatttatta aattcttcta ttcggtttgt
     8401 gttatgcttt aacttctgag atactttttg cgccttttcc tggtcattcc aaaatccggg
     8461 ttcctgcatc tgtgattcca atgtaaaatt ttcttccctc aacttttcca agtcaaaggg
     8521 aaccacctac ctcgtttaat atatcattga actttttgat tctttcagta atttcatacc
     8581 aatcagtcaa tatatcatca tctccttctt aaatattcat aacttgtcat cctgagcaaa
     8641 gcgaaggatc tcatttttac atgcatgaga ttcttcgggc taaagccctc agaatgacaa
     8701 ttcataaaca cacagcaata acactatttc tcatttgctc cgcagcattt tttatacttt
     8761 ttcccgcttc cgcacggaca cgggtcattt ctgcctacct ttttctcttt tttaacaaga
     8821 ggctgaggcc tgcccccaat actgtctgca tctgctccgc tgacacttgt aattcttgct
     8881 acttgttttc gctgcaattt ttcctgaggt cttactctca tgagcatttt taccgtatct
     8941 tcctgaattg cttcagtcat ttcttcaaac atatcaaatc cttctacctg atatgctctg
     9001 acagggtctt catttcctat ggcacgaagt ccgattccct gtttcagttg gtccatagcg
     9061 tctatatggt ccatccattt tgtatcaaca tttctaagaa gtacaattct ttccacttct
     9121 ctgaaagttt cttctccaaa ctctgcttcc tgccttgcat aatgcttgtc aacggcaatt
     9181 ctgactcttt ttcttaagtc ttccttgtct atatcttcgg gttttactcc gtcaaagctg
     9241 attaacagtg agaatgtttc tgctatatac ttgacaaatc cgttcaaatc ccaattttca
     9301 ttattctttt cgtcggtcgt atacatgttg attgccttgt cgattaagga atcaatcatg
     9361 ttggaaatat attcttttaa gtcttctcct aacagtaccc ttcttctttc ggaataaatt
     9421 atttctcttt gcttattcat tacattgtca tattggagaa catgttttct tatatcaaag
     9481 tttcttcctt caactcttgc ttgggctgtt tcaatggtcc tggtcagcat tcctgcttct
     9541 agaggctcgt cttcaggcat ttttaatgtt tcgattattg ctttaacctt atcaccggca
     9601 aacagtctca ttaaatcatc ttccagggat atataaaacc tggatgaacc cgggtctccc
     9661 tgcctgcctg aacgacctct gagctgattg tcgatacgcc ttgattcgtg gcgttcagta
     9721 ccgataatat gaagtccgcc tgcttctatt accttttttt gctcgtctct caattcgttt
     9781 tttgattctt ccaataactg cttatatact cttcttgctt ccaatatttc agcatcatct
     9841 gtttcattga aaccgtcagc ttgtgcaatc agctcatcgg aaaatccttt tgccttaagt
     9901 ttatgctttg caataaattc cggatttcct ccaagcacaa tgtcggttcc tcttcctgcc
     9961 atgttggttg caattgtaac agagccgaga cgtcctgcct gggctacaat ttcagcttct
    10021 ttgtcatgct gctttgcatt taatacttca tgcttgattc cttgcttttt taaaagcttg
    10081 cttaaaattt ctgatttttc tatagaaata gtaccaacca aaatgggctg tcctgtttgg
    10141 tgcctttcaa taatttcgtt tattacggct ttgaatttgc ctgcttctcc cttgtagaca
    10201 acatcctgca aatcttctct tattacgggc ttattggttg gaatttctat tacatccaca
    10261 ccgtaaattt cacggaattc attttcttcc gtctttgctg ttcctgtcat accggacagt
    10321 tttttataca ttctgaagta attttgaaat gtaatggtgg caagtgtttt tgattctttt
    10381 cttacttcta ttctttcctt tgcttcgatt gcctgatgaa gtccgttgga atatctcctg
    10441 ccgtacatta tacgtccggt aaattcatca acaatcagaa cttctccgtc ttttacaatg
    10501 tagtcgatat ctttcttcat cgtattatgg gcttttaagg cttgatttat atgatgggtg
    10561 agctccatgt tttcctggtc ggctaaattc tcaacaccga aatatttttc tgctttatcg
    10621 atacctcttg cagtaagggt aacatttttt gccttttcat cgactatata atcaacagta
    10681 tcatcttctt caacatcccc cataatatag tccatctttg ttttcttttc ttcaggcagt
    10741 tgaatttttc ccttcaaatt gagtacaaag ctgtttgcca tttcatatag gtcggttgat
    10801 ttttctcctt caccggaaat aataagaggc gttcttgctt catctataag tatagagtca
    10861 acctcatcta taatgcagaa gtttaaatcc ctttgaacca tgtcttcctt atgaataacc
    10921 atgttgtctc ttaaataatc aaaaccgaat tcattgtttg ttccgtaggt aatgtccgac
    10981 ctgtatgcac taagcctttc ttcctttgta atgccgtgga ctatacagcc tacagaaagc
    11041 cctaagaaat tatgcacctt tcccatccag tcggcgtcac gccttgccaa gtaatcattt
    11101 accgttacaa catgcacccc gttgccttcg agagcattca aataaacagg caatgttgaa
    11161 acgagtgttt ttccttctcc tgttttcatt tctgcaattc gtccctgatg aagaactatt
    11221 ccgcctataa tttgaaccgg ataatgcttt tgacccaata ccctccatgc ggcttctctt
    11281 actactgcaa atgcttccgg caatatatca tcctttgttt ctccatttgc aagcctttgt
    11341 ttgaattcgg cagtcttacc cttgagctgg ctgtcggtca aagtttccat tgtcttttcc
    11401 aaagataata ccttatctac gataggttgt attcttttaa tttctttttc gctgtatgaa
    11461 ccaaaaattt tttcaaaaaa atttttcata ttagtatcct tccatacttt attcaagaga
    11521 gctgaaatat ccggcagtgt agcctgaact ccatgcccat tgaagattaa atcctccgca
    11581 gtctccgtct acatcaagaa tttctccggc gaaatacaat cctttcacct ttaaagactc
    11641 caaggtcttt ggatttacct cagaaagctt aatccctcct gccgttgact gagcctgctg
    11701 ccatgaatta tgtcctataa cttcaaattt ccactctttt aaaatctcta ttattttata
    11761 tatttctttc ttgtttaatt tgccgcatat cttatttaaa tcttcaaagc ctgcttcata
    11821 aaaaactacc ggaattaatt ttttgtttat aaaaccgatc atagactctt caattgtttt
    11881 atagtttatt cttctgaatc tgtcctgaag aatatcataa agcttaagct ttgaatatcc
    11941 gggaaacata tctatgttta aaaaaggttt atttttctta tttagctcct caataacctt
    12001 tcggcttacc tgcagaatcg gaggtcctga tatgccgtaa tcggtaaaaa gtatctcccc
    12061 ttcttcttct cttataagcc tatcgcctgc ataggctttt acgataccgt caaattttat
    12121 tcccgcaatt cttttaaagt acttaccgct taattttaac tgaacaaggg caggaaaggg
    12181 ctctattatt tcatgtccca aggatttggc taattcatat cctttcccgt cagagcctaa
    12241 ctgaggactt gcttttcctc ccgttgccaa aataaccctg tctgcagtaa ttgtatcatt
    12301 cccaataata ctaaatttat ctttgctttt ccttaaggct gttacgttaa agtcagtaac
    12361 ttccttgacc ttatgtcttt ttaattcata tcttaaaaca tccagtacgc ttgaagcctg
    12421 gagagaattt ggatatactt tgccgctttc atcaacgtaa ggcaaaattc ctaagtcttc
    12481 aaaaaagaca agagttttat caagattaaa aaatttcata acttcatcta caaaattcaa
    12541 attattagag tggtagcatt cctttgacag attcatattg gtataattgc acctgccgtt
    12601 gccggttgcc agaatttttt tgcctactct gttcaatttt tcgcagatag ttacttcagc
    12661 accgtttctg gccgcagcta tcgctgcggt caaacctgat gcacctccgc cgattacagc
    12721 aattttcata gtttattcct tatcgttaat ggttttgtat gttttaagaa tttgctccgc
    12781 taccttaagc ccatcgattg atgcagacat aattcctcct gcataacccg agccttcgcc
    12841 gcagggataa atccccctta catttgattg catttcatca tcccttaaaa ttcttaacgg
    12901 tgatgaagtt cttgtttcaa ctcccgttag cagtgcatcc ggtctgtcgt aatttgttaa
    12961 aaattttcca aaagctgtta ttccttcaac caggctttca cacacataat caggcatgca
    13021 atcccacagg tttgcaaagg tacagcccgg tctgtatgta ggatatattt ctccaaaaga
    13081 tgaagaaaca attttttctt taaaatcttt aaagagctgc accggagccc gataatttcc
    13141 tccgcctgcc tcaaaggctt ttctttccca gtatctttgg aattctacac ctgccattgg
    13201 attttcttcg ccgccgaaat cttcgggatt tatttgtaca accacggcag catttgcatt
    13261 ttccaaatcc ctttcgtgaa aactcattcc gtttgtaaca actcccccgg cttcggaggc
    13321 agaggcagtt acaactccac ccgggcacat gcaaaatgta tatacgcttc ttccattttt
    13381 acttttatga acaaatttat agtcagcagg acctaagagt ggatttcctg ccatttctcc
    13441 gtactgagct tcgcatatca ttttttgagg gtgctctacc ctgacaccaa tcgcaaatgg
    13501 tttagctgtt agttttaact tattattcag catttcaaat gtatctcttg cgctatgtcc
    13561 tattgcaagt accaatatat ccgtttcaat aatgtcactt tcggtaacaa tgcgttggac
    13621 agcattatcc ttaataatta tgtccgttaa acattcttca aacctgaatg ttccacccag
    13681 acttattatt ttttttctta tatttacaag aacatttcta agaaggtctg ttcctatatg
    13741 aggtttgtta atatatgtga tttccgccgg ggcacctgct tcaacaaatt cttccaatac
    13801 ttttttgcct ctttttgacc tgtccttaat taaagtgtta agtttaccgt cggaaaatgt
    13861 tcctgctcct ccttctccaa actgcacgtt tgagctgtag tttaaaatgc cttctttcca
    13921 aaatttttca acatctttga ctctttcctt aacagtcttt cctctttcga gaattatagg
    13981 tctgtagcct ttctctgcaa gaagcagtgc gcaaaaaagc cctgcgggac cgcttcctac
    14041 cactacaggc ctgttgttta agggcttttc tcctgtagct tgcacatcat atataaatgg
    14101 ctctgcaacg gaaatgttgt tgtttctttt ggaaattttc acttccttgt atgtatcaac
    14161 atctaatgta taaataaaaa gtatttcatc ttttttcctc gcatcaattg attttctgaa
    14221 aatagaaatc tccttgattt cttctttctt aattcttaag gcttctgctg cttttattaa
    14281 cagctcttcc tccttgtgat ctataggcag cttgatttgc tgaattctat acatatcgta
    14341 cacctcttta acttcctgtc atttaaaatt ctgccatgta ctatatcaat atccttatcg
    14401 atctatcggg cttgacataa taatatatgt ttccgtattg ccgtaaacct gaatttctct
    14461 taccaattct tctaaatgct tcatttctct taagtttgct tttattatca tgctgtgagg
    14521 acctgtaacg tgatggcatt ttgctatttc ttccttgttt tcggcaaaat gcaaaaaatc
    14581 cttctggttt tcaggtttaa ttgatgcatt tattaatacg gtaataggct tacctatttt
    14641 ttcgttgttt attacagccg tataacgctg aattacatcc gtttcttcta atttttttac
    14701 tctttctgcc acggcaggcg gactcaatga tactattttg cctaattcct tcattgaaat
    14761 tcttccgtta ttctgtaata tgctgataat ttttaaatct gtctgatcca ttttacacca
    14821 ccttattatt atgtatactt aaaaacagat tccttttaaa agggttgcaa aaattaaaaa
    14881 aattaaataa tgtacaaagt cataaaaata tgctattgta tttattaaaa tgataatcgc
    14941 tgattatcat tatcatcaaa atacaaaggt attataccat aagtgcaata atcatatcaa
    15001 ggaaaagaaa tgagagataa ttttaattta acataataaa aggagaagga gaaaatatga
    15061 caaaacttaa aattactgaa acatctctga gagatggaca tcaatcgctt atagcaacaa
    15121 gactttcaac tcccgaaatg ctgccaattc tgcaggaaat ggataaggca ggataccatg
    15181 caatggaagt atggggagga gcaacatttg atacctgcct caggtattta aatgaagacc
    15241 cttgggaaag acttaggaaa attagagcaa atattaaaaa tacaaagctt caaatgcttt
    15301 cccgcggtca aaatttattg ggatataaaa attatcctga tgatattgtt gaaaggtttc
    15361 ataaaaatgc aatttctgaa ggaattgata tagtcagagt atttgatgct cttaatgaca
    15421 taagaaactt acaaaaaaca atagatacag taaaatccga aggtgctcac ctgcagtgtg
    15481 caatatccta tacaatcagc cccgtacata caaaggagta ttatcttaaa ttgattaaag
    15541 actttgaaaa tgcaggagct gactcagtat gcataaagga tatgtccggt atattgcttc
    15601 cttatactgc ttatgattta gttaaatcca taaaggaagt tactaacctg cctattgagc
    15661 ttcataccca ctgtaccgga ggaatcgggg aaatgactgt tctaaaagca gtagaagcag
    15721 gagttgatat tgttgacaca gccatatcgc ctttatcatc aggaacttca cagcctgcaa
    15781 ccgaatccct ttgtttgacg cttaaggata cggaaagaga tccacagctt gatttgatga
    15841 agttaaatga aattgcagat cattttaaaa atgtcatgaa aaaatttgaa gaggaaggaa
    15901 cattaaatac aaaggtactt atgacagaac ctaagacctt gttatatcaa atacccggag
    15961 gcatgctttc caacctgctt atacaaatga agtctcttaa tgcagttgac aaatttcaag
    16021 ctgtgttgga tgaagtgcca aaggtaagag aagacttggg atttcctccg ctggttactc
    16081 caatgagcca aatagtagga gctcaagcag tgtttaatat agttacggga gaaaggtaca
    16141 aagtaattcc tacggaaata agaaattatg taagaggtct atacggaaaa cctgcagctc
    16201 ccatatccga tgaaataaga aagcttatta tcggagatga agaagtgata acagtacgcc
    16261 ctgcagacct gttagagaac agctatgagc aaatgaagga agaaatagga ggacttgcaa
    16321 agtctgagca agatgtttta tcctatgcac ttttcccgcc ggttgcaaaa gcattctttg
    16381 aaaaaagagc cggcaaataa cgggacagtg gggacgtacc ttggtgtccc actgttttct
    16441 tgttttctga cccgtttcct ttcaaatagg cagttataat tgttaaaaca aaaaacctct
    16501 ggagattgac ctaaaaagtc cacctccaga gttttttaag gtgacttaat ctttttttca
    16561 tgttcgctat ttcttattcc gtaacatttt tattcatgcc acaacatttc ttgtattttt
    16621 taccgctacc gcaagggcat ggttcattgc gtccaatttt attagacttt gtcattttaa
    16681 ttattttggc ttttgatgta ttagaatttt cttcttgcaa taattcgctt ggagtataac
    16741 cttttagcgc ccattgacgt gtattattgg ataactcaat tactttcaga gttaatactt
    16801 gtttatattc taatgaagga atctctaacc atgtttctaa atacttaata ataaatgttg
    16861 gttctgaatc ctgatttatt aaatttgtta attgggttga tatttcatct atatcatcat
    16921 ctttcaagtc ataactttcc agtaaaaaat caataaaact attcatttca ggagaagtgt
    16981 ttttatgatt ttgattacct gcctttaaaa gttgactttt agtaaattga taataatcaa
    17041 gttcaggtct tttattatgt tcttctataa tattttcgct gtcaaatact ctcgcatctt
    17101 tataaccata tgatggtatt ttatccaaat tttcatataa attgcaagca agtgatatga
    17161 cttctgcaaa ctgattaata tttatattct ctttaacata cttctgtaca tttttaatga
    17221 gtctccatga atccattaca ccatagtaat atagcattcc ttgtgttaat tgtatccact
    17281 ctgtatttct atgaataact ttttgtcttt cttcagtatc taatatgttg aatttttcca
    17341 ttaattcaac cggcatgaca agtatccttt ctccgttctt aagtccggga aaagttattg
    17401 catacctcat taagttctct attatagaga attttatatc tttgcaaggt attgctcctg
    17461 aattttttac tattgatttt attaaatcat aagtgttttg atctaatata cgtattattc
    17521 cttcaaattt atgaggtatt atttttatta gctcagatgc taattctgac tttttcaaag
    17581 agcttaaccc ttgtaattta tagtttttac aaatatgtga catttcatcc ttagttaacc
    17641 tacaaattac atcatttaag ttgcatggaa tttctatttc actaaaaagc ttttcctctc
    17701 ttctttcttg cagatattgc atatgttttt tagcgtcttc caaagccttt agtatgattc
    17761 tagctgtttc tttatttaat tcttttttca ttttcaaccc cttggggacg taccttggtg
    17821 tcccactttt ttaagtatct catattttcc ttatttattt taagtgtttc atttattata
    17881 attgcgttat ttgcaccttg atattacacc tctggatatt cccgtagccc tctctatttg
    17941 tctgattgaa aacttttcag tggttattaa ttgcttaatt attctattcc tataatcaat
    18001 tggcatttct cctaacttag taggttcctt accttttaat atttccttta ttctcttttt
    18061 tacaaattca tcatcaaatt tttgtatttt atctgatatt tcatattctt cttcattaat
    18121 ttcaaaatta aattttatga aactgtctct atcaattatt gacattataa aatctgtatt
    18181 gcatatttca ctctttccca aatattcttt gaagctgctc cacttataac tgtctatttt
    18241 agatgttagt ccgcccttga caggattttg atgtatatac cttgctaagg ttaataagta
    18301 atcttctgtt tcaacaggat cgcttttaaa tctgtttgca attagtattc cacttctatc
    18361 atattttcgg ttaaaccagc ctgcatatct tgtaagtatt ctttgcatta tcaatgatat
    18421 ttcacccaat tccaattcct ttattattag gtgcacatga ttgttcatta aaacatatgc
    18481 ataaatatca aagctaattt tttcttttaa atcagaaata atttctaaaa ttttctcata
    18541 atctttatct tcctcgaaaa tattgaacct gttaactcct ctaaaaatta catgatatat
    18601 tccagtctca ctcaattttc tggcaaccct tggcatgaag atcacctgcc tttttgcttt
    18661 taattatatg atattttcca tataatgtca aaatgttaaa atgatttcaa atatatgcag
    18721 tgggacagca aggtacgtcc ccactgtccc gctagaggtt ttttagttgg ttttgcatta
    18781 gaacaccgac tatttctgca aatctttcgg gataatatat acgggcaaag ttatgtccgt
    18841 atattttttt tgctgtcggt atatcctcat cttcgccggt aaagacgcaa agaattgaat
    18901 tgccgttatt tatgcctctt ttcacttcaa aggcagtatc tattataccg gtagcccccg
    18961 catacttggt ctgctcctgc tttattcccg tgtcgggaat gcactgggtg tcattaggct
    19021 tgcagtctga aagaacaatg agaattttat ttaaataatt gctgccttcc atcatgtaca
    19081 atgccgttct tattgccaag ccgtcacggt tgcagcccgt tgttttataa ttgaatattt
    19141 tagtgttttt atctgtttca tcataatcac ggtaaagatt tataacggtg taatttctta
    19201 ggcttgaaaa tgagtatact ctgacgggaa tttggcactg agtaaagctt tctgcaataa
    19261 tatacgcttc cgctgcgatt gtttcctgtc tttcatgctg cgatgcggat gcatcaagca
    19321 aaatatctac cgataaattt ccgatatcgt tgttatatac tttcgtgaaa actctgttat
    19381 catttaaata gacatttctc caaatttttg gtgcattaag tcttcctgct tcggattttc
    19441 cggtatatga ttcaaaatat gcaagcatag tgtttctgat tttatttgta agctttaaaa
    19501 tactgttttt gtattttata taattggaat tgaaatgctc taaattaatt tccctttgct
    19561 ctttcaatgc tttattgaaa tacaatgcat taatatcatt gctgaattca tcatcaaatt
    19621 ctcctctggt gaaatgaaga tgagtatttt tatggttacc gatacaaagt tttcgctcta
    19681 ttaaatatgt ttttttatcg ggaaaaattg aagcaccgaa atatttacga atatactgtc
    19741 tttccgaatc atctttcttt tcaattttaa ctttcttagc tgatttttta atttttccct
    19801 ttttttcttc atagaaaact tctctcgacg tttcagctga atccaaatac agctctttca
    19861 tagtttccat gtctgaatcg ctaagtcttt cttcttcgca gtattggatt tgtctttgca
    19921 ctcttttttt gtttatttgg gcgtttactt catattgacc gtaattgaac ctgaaatatt
    19981 tcttgattat ttggttcatt tttaaaatta tttcttctgt tgaagcatca ccgaattcaa
    20041 ggtcgtttaa aatatttaaa gcattgcctg ttattcccgg agctttacct aatgctcttt
    20101 taaagtgagc atttgtcagt acatttaata aattatcatc aaaaacttca gtttcacctg
    20161 ctaatacttt tcttgcataa ttttttctaa ggcttactat tacaggcctt tcgttttttc
    20221 ctttatcaaa tgcactcttt tccaagccta tccagaaaag tttttcataa aattcatgct
    20281 ctctgtctgt tttaagataa ttaaaaaagc ctctcagcaa agtgtagtca tagtatttgt
    20341 gaacagcccc tattatataa ttccaataga tatccgcttc accgttttta tcataagcct
    20401 tgaattgagg ctcgatggaa tagtcgtccg atgcattcca aataatattt agagctcttt
    20461 tatcgtcttg tgctgtgttg ttcatgctca cctaaaaatt tcattccttc ctaaattttc
    20521 agctattcgt aggcgtataa catcctctac aagcgttcgc tcgaaatcat caaatgattt
    20581 gtttgtaatt cccatctgca atgctttgaa aggagatagt cctttatcca ttaaattaat
    20641 tgaagctaaa agtcccctca aatcaaccga cttagttgaa atctcggcat tttcgctttt
    20701 taactgcaaa tccttgaaaa gccctgcaaa ctgctcaaca tattcttctc ttaaatttgg
    20761 atattctctt tgcaataatt ttcttaaatt ctttgatgaa atattaggca tatttattac
    20821 catgaaacgg gatgtcaatg cttcgtttaa ctctcttgtg ccggcataac cgtaattcat
    20881 ggttgcaata aaacggcttg cgtcatcaat tgtaattttg tcatatccgg gcacgtcaat
    20941 tattcttcta tagtctaagg ttgcatgaag tactgcaacg gaatcatttt tggccatgtt
    21001 tatttcatcc aatacgccaa agccgcccca ctttgcacat tcgtagatgg gaccgtttct
    21061 gaatacaacc tgaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctaa
    21181 aatataatgt ccataacttg tcttatgttg tttaccggga ttatttccat gtttttaact
    21241 tcttttgaaa tttctgcatt ggcttctgct atgaccgctc ttttaaatcc catctttttt
    21301 gcttccaaaa gtcttttttc aattgaatta actcctctta tttctccggt tagccctacc
    21361 tcgcctataa gaacggtctt aaagtcgacg ggtatttctt tgaagcttga tgccaaagcg
    21421 catagtattg ccaaatcgca tgcaggctct ttgatttgca gccctccggt tacattaata
    21481 aatatatcgg agctttgaat tgacagacct gctttctttt ccaatacagc aatcagcatg
    21541 gatgccctgt ttttatcaat tcccgtggtt accctgttgg caaatccaac aggagaatat
    21601 gttaccaagg cctgaatttc tatcagaacg ggtctcgtac cttccattgc tgcagtaact
    21661 acggtgccgt aagcatcaag aggtcttccc ttcaaaaata cttccgaagg attttcaacc
    21721 tctataagtc ctctgtcccg catttcaaaa atacctattt cattggttga gccaaatcga
    21781 tttttaacag ctcttaatat tctatatgaa aaatgcctct ctccttcaaa gtataaaaca
    21841 gtatcaacca tgtgctcaag tactcttggt cctgcaattg cacctgattt tgtaacgtgt
    21901 cccactatga aggaagccat atttcttgtt ttcgtaattc tcattaccat tgaagtcact
    21961 tccctgacct gggtaacgct gcccggggca ctgggcacat cgggtgaata tatagtctga
    22021 attgaatcaa gtacaagcaa atccggctct tctttatcta cgtgctcgat tattatgtca
    22081 atattcgttt cagcaagaac atacagctca ccgtctccta tccccaacct atcggctctt
    22141 atttttatct gctctcccga ttcttctccg gatacataaa gtacttttaa tttttgcttt
    22201 gcagcgtggt ctgcaacctg cagaagaagt gttgacttgc cgataccggg atctcctcca
    22261 actagtatca gggagctttt tattattcct ccgcccaata ccctgtccat ttcaacgctt
    22321 cctgttgaga ttctttcttt tttggtggat gtgatcttgt ttattttttc aattataccc
    22381 ttgctgatac ctctttgact tttggagtca ctttttttag tttcaaattc ctccacaaag
    22441 gtattccatt cttcgcagga gggacatttg ccaagccatt tggccgtttc ataaccgcat
    22501 tcctggcaaa caaatttgga ttttatctta gccatgattc acctgctttc actgctttca
    22561 tacgctaatt aactataacg caaacctgta taatatgtca atagtagtat aaataacatc
    22621 cttgcctg
//
LOCUS       DAOPUE010000024        21937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2373_length_21937_cov_15.430582, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000024 DAOPUE010000000
VERSION     DAOPUE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_21937_cov_15.430582"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKH42_03675"
     CDS             <1..345
                     /locus_tag="PKH42_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA19563.1"
                     /translation="QSLIHFGEDFTSAGHLYLARIPNISENERNEIIIKMAENGISTN
                     VHYKPLPMLTAYKNLGFDIKDYPNSFDMYKNEITLPLHTLLSDEDVKYICESLKKAMN
                     KVLQTRQVVADK"
     gene            356..982
                     /locus_tag="PKH42_03680"
     CDS             356..982
                     /locus_tag="PKH42_03680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008496964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOA19564.1"
                     /translation="MYRKYFKRIIDFVLAVIALPFWFIILIIIGAIIYIQDEGPIFYN
                     AQRLGQDGKIFKMYKFRSMKVNSLDFRNEDGSTFNSEDDPRLTKIGKFIRKTSLDETP
                     QLLNIIKGDMSIIGPRPDLPEHRDLYEGNEERKLEVRPGVSGLNQAYYRNTVPWKERI
                     QNDIYYIDNLTILLDIKIFLKTVSSVLKRESVFIEHKSKSKSGVKANG"
     gene            975..1697
                     /locus_tag="PKH42_03685"
     CDS             975..1697
                     /locus_tag="PKH42_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19565.1"
                     /translation="MANTNYECKHLQWDTDYFGVSSARVNLFGIVDEKGQEDIVEFCK
                     GYDFVTISNLDNTKENNHWIGNRTNAFLTDINVKFIKVLEDKPDYQDEKTYTANCLYK
                     NAQIVDIAGKSFNYSRFFNDSKLPEKKAKNIYIHWTECAFNNKDKYFVISEREGNVAG
                     YILFSFIEDRSVIELIAVDEKYQGQRVGKSLINTMESFVIDKGIGKIKAGTQVNNISA
                     TRFYSKMGFEYVSCGSVYHLWR"
     gene            1716..2936
                     /locus_tag="PKH42_03690"
     CDS             1716..2936
                     /locus_tag="PKH42_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA19566.1"
                     /translation="MKVLYIATSFPEPNKGATIYTDLAEALYEAGHEITVAVSEQAKN
                     LSRTQMKKERGFDVLRIVTGNYYDVSLIEKGITTLRIPIIMKNEISKYIVNRKFDFIL
                     YEAPPVTNASLVAWAKKKFDCPSYLMLKDIFPHNAVDIGIMKKSSIIYRYFIKKEKDL
                     YKSADIIGCMSEANKQYIIKHNSWLNESKIELLPNTKRITDEIEPVKLKIRQQYSIPE
                     DACVFIFGGNIGKPQYVDLLSQAIIECKNDEGIYFLFVGRGTERYKLENTIKSENIIN
                     AKVIENLPRDQYEQITKESDVGLIILDPRFTIPNYPSRILSYMEYSKPVLAATDKTTD
                     IKDLILQSKCGEWVWSGDKDKFVEAIRRMSKSPLLKQMGRNGRDYVKKNFSLESSVDI
                     LEKRFNKETRQQNV"
     gene            2929..3972
                     /locus_tag="PKH42_03695"
     CDS             2929..3972
                     /locus_tag="PKH42_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOA19567.1"
                     /translation="MFKDKTMLITGGTGSFGNAVMGKFLKTDIKEIRIFSRDEKKQDD
                     MRRHYKDDKLKFYIGDVRDIQSVKDAMHGVDYIFHAAALKQVPSCEFFPLEAVKTNVL
                     GTDNVLTAAIEHFVKKVICLSTDKAAYPINAMGISKAMMEKVFIAKSKTVDADKTLIC
                     GTRYGNVMASRGSVIPLFIDQIKSGQPLTVTDPNMTRFLMSLEEAVELVVFAFQNAEA
                     GDIMVQKSPASTIADLAQAVKELFNADNDIKIIGTRHGEKRYETLLTKEEYIKAEDMG
                     SFYRVPADKRDLNYDKYFIEGSQKLSSNGEYNSDNTERLNIEQIKEKLLLLECIRKEL
                     EYFNYRYETAITM"
     gene            4006..5121
                     /locus_tag="PKH42_03700"
     CDS             4006..5121
                     /locus_tag="PKH42_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOA19568.1"
                     /translation="MKILITGAKGFIGKNLITELKNRKYTDLFEYDIDTNPVLLDKYC
                     KEAEFVFHLAGVNRPKEQSEYMEGNFGFTSILLDLLRKHKNICPIMLSSSIQAELDNP
                     YGKSKKAVEELLIDYGKKNGVKVLIYRFPNVFGKWCRPNYNSAVATFCHNVAHDLPIT
                     VNDPSVVMKLVYIDDVVNELISAYEGKENRKIEFCEVPIVYTITLGEIAKLLYSFKKS
                     REGRSIPNMSDVFIKKLYSTYLSYLPEDKFSYELKMNVDNRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHSKNEKFLVVSGKGVIRFRKIYSDEVIEYFVSGDKMEAVDIPPG
                     YTHNIENLGDIDMVTIMWVDESFEPEKPDTCYLEVTM"
     gene            5118..6242
                     /gene="wecB"
                     /locus_tag="PKH42_03705"
     CDS             5118..6242
                     /gene="wecB"
                     /locus_tag="PKH42_03705"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOA19569.1"
                     /translation="MKRLKVMTVVGTRPEIIRLSAVINKLEESKVIEHVLVHTGQNYD
                     YELYEVFFEDFKLKKPDYFLNAANGTAVETIGNILIKIDPILEEIKPDAFLVLGDTNS
                     CLCAIAAKRRHIPIFHMEAGNRCFDQRVPEETNRKIVDHISDINLTYSDIAREYLLLE
                     GLPADRIIKTGSPMFEVLNSRKTDIEKSDVLERLGLEEGKYFVVSAHREENINSEENF
                     LDLIESLNEVAEKYKMPVIISTHPRTRNMIFTRGIEFHPLIQTMKPLGFNDYIKLQIN
                     SKAVLSDSGTISEESSILGFKALNIRQANERPEAMEETSVMMVGLKRERILQGIEILE
                     TQEKDTLRKVGDYCMPNVSDKVLRIILSYTDYVNRVVWGK"
     gene            6278..7372
                     /locus_tag="PKH42_03710"
     CDS             6278..7372
                     /locus_tag="PKH42_03710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOA19570.1"
                     /translation="MKLLLIAPCPPPYGGISNWSKIVIDGIRRYFADIDLDVIDISPR
                     RRETEGRNLFNRAMDGITSTIKALCIAKKTLKNSKPDSVHLTTSGSLALLRDYVLLIF
                     FRKHQINTVYHIHFGRTAEIIKYGSWERILLMHAMKHAGSVIILDRLSFNSLREQFPL
                     LQVKKINNPIKIMGEPRNSTSKYYMFLGWVLKTKGIEELVSAWNQAGINATLRIVGPY
                     QESYLEYLHQAGYDFKSIIFEGEKGHDEAMEILSECEAFILPSYSEGCPYVILEAMSF
                     KKPIIATNVGDIPNMLSNECGILVEPHSSTQICEAIKSLESDSDLKIRIGSNAYKRVL
                     EEYSIKEILDLYRNEWESKYESIPFLLNIS"
     gene            7405..8505
                     /locus_tag="PKH42_03715"
     CDS             7405..8505
                     /locus_tag="PKH42_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA19571.1"
                     /translation="MKKVLIVIPTVRTGGAEKLALDISRLLDKNKFEATLLCYYSDQP
                     DAYESLIRDENIKIVYLNKKLGLDFTMFSKVKEVVELISPDIIHAHLDTLLYLIPSFN
                     RNQVKLFTIHSTPCYEASGILQKIIRTFCFHYKNVIPVAISQCFVQDIASYYQLNEKR
                     IACIPNGIDFGNFNTSKRKKTEDNIYKFITVGSMRPEKNQKLLIKAFAYVRKKNPNTQ
                     LTIVGDGTLKPEIEKLIAAYHLQDAVNLTGIVSNVGDYLASADAFVLSSDFEGLPLSL
                     LEAMACGLPIVSTKCGGTVDIIDDGVNGYLVDINDIGQLSDAMYRVTLLSEELRLRMI
                     EKSFEILPMYDIKRCTGDYERLYTKNIVTMLK"
     gene            8524..9672
                     /locus_tag="PKH42_03720"
     CDS             8524..9672
                     /locus_tag="PKH42_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA19572.1"
                     /translation="MNDTIATCKSKHPISVRCLFAWLVLMPFSFVALGSLGSILKLLT
                     IVIIVYYFIKGGRLKYRKSAILFTWFLYVIYYAVSLIWSLNLSDAIVYVIGYIQFFVL
                     AFLFSSEIMDNDDIRHLEDAWIFISIFCIIIFITGGDKSIEYSDRSTLYMLGTFTDPN
                     EFGGYFCIPTGLMCSRLFSENKRKSCIYLILIIVSTYIVLRTGSRGALLALIVCIIAA
                     MFRAARMSFKNTLFIIVLAILSYFIILNFMLPQISESVLNRFTFESLKADGGSERKLI
                     WTKSIKYFYEESIFRQVLGLGIFGGTFYKATMHNQLLQNLIDGGFIGLLLYCVLMVLG
                     FINIYKKYKNFVPGYIGMMLLSMTLTMTSSYKPLWTLMILGLIDIRRE"
     gene            9834..10841
                     /locus_tag="PKH42_03725"
     CDS             9834..10841
                     /locus_tag="PKH42_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA19573.1"
                     /translation="MMKVLVTGGAGYIGAVLVRKLLKEGYEVRVLDSLKYGGESIIDL
                     LNNPKFEFVKGDIRDREIAYASTKNVDAVVHLAAIVGDPACSQFPVEAKEVNYDASAQ
                     LYEESLKNNVGRFIFSSTCSNYGKMDNSEKFLNEESELKPLSLYAQTKVEFEEYLLNQ
                     DKINVCKPVILRFSTVYGISPRIRFDLTVNEFTKELAMGRELLVYGEQFWRPYCHVDD
                     LANSILFVLESDEAKVEFNVFNVGSSDENYQKKMIINEILKQVPDGMVKYVTVNEDPR
                     DYRVNFDKIRDVLGFKVTKRVPDGINEIVTLIENGFIINPDDDKYYNIIRVRKPAILE
                     V"
     gene            10843..12015
                     /locus_tag="PKH42_03730"
     CDS             10843..12015
                     /locus_tag="PKH42_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HOA19574.1"
                     /translation="MAQRFIPLSVPNLKGRELQYVQDAIETEWVSTAGKYVNKFEEKI
                     ISYAITKGAVSCQNGTAGLHTALIVSGVTKDDEVIVPALTFIASVNPVKYIGAEPVFM
                     DCDDSLNMDTDKLEDFCRRECIFADGSLINKQTKKHIKAIIIVHVFGNMADMESIIEI
                     AREYNLIVIEDAAEAIGTYYTQGKYEGRHAGTIGDAGVYSFNGNKIITTGGGGMIVSN
                     DEDLLKRAKHLTTQAKTDELYYTHDDVGYNYRMTNVQAALGLAQLEQLETFIKIKIEN
                     YILYKEKIHEIPGLSLLNFNEDTRSNHWLYSLICGDEYPLNRDEIIMYLSSKGVQARP
                     IWGLINEQIPYLKNQAYKIEKAKFYVDHVVNIPCSTNLTKDEILYVVENLSNAALK"
     gene            12029..13075
                     /locus_tag="PKH42_03735"
     CDS             12029..13075
                     /locus_tag="PKH42_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOA19575.1"
                     /translation="MEIKDILIDENSTMIEAMNALDKTAKKILFVVKEDRLVAAITDG
                     DIRRWILAKGSLDARVKSIANYKPLFLYEKDKALARKYMKQNLIDALPIVDENKKIIT
                     VKFLYRQDIKNKVELDCPVVIMAGGLGSRLYPYTKILPKPLIPIGEIPIAEHIINRFQ
                     QLGINNFYMIVNHKKNMIKTYFNEIAKNYKIIYIDENQPLGTGGGLSLLKGIVKETFI
                     LTNCDIIIEEDIGKIYNFHKEKNNLITMVCSLKNITIPYGVINISEDGRIEDMKEKPV
                     LKFFTNTGMYVVESDIVNELENNKTIDFPDIIEQYKKSGGKIGIYPISEYSWMDMGQI
                     DVLENMRRRLENEM"
     gene            13065..13715
                     /locus_tag="PKH42_03740"
     CDS             13065..13715
                     /locus_tag="PKH42_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOA19576.1"
                     /translation="MKCKKLLLIGGGGHCKSVLDSILDNNDYIDIGIIEKKEICYGDI
                     LGISIIGCDSELEYYYNNGYQFAFVTVGNIGNPKIRIKLYKLAYKIGFKIPNIIDSSA
                     CVSRFTNLGRGIFIGKQAVVNAGTSIGDCAIINTSSVIEHDCIIEEFVNVAPGAVVCG
                     NVTVGRNSYIGAKSVIRQQLKIGSNTIIGMGSTVTKNIDSNVLAYGNPCRVINSKY"
     gene            13728..14606
                     /locus_tag="PKH42_03745"
     CDS             13728..14606
                     /locus_tag="PKH42_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOA19577.1"
                     /translation="MKTALGTVLYKQSFKYLNEFSRSVNNQDCPDFDMLFLNDDLDEA
                     EIDISINSIKKKVILSSGKPNSTIPQLRYQLIKYAKENNYDLLALVDFDDTFSENRVS
                     KVKTAFNNEYGFYYNDIYYFYKKNKFFSFLPDYTNEVNQILEQNYLGLSNTAINLSKI
                     DFDLLEKLNVTICKAFDWYMYSVFLAEGHKGKKVDGCRTYYRIYENNTAGECKNTINY
                     INNEINTKINHYSLLKQWDSKYEELYDYYKALQHEWKRGNVDLLSYIDIKNDYWWGLI
                     KSNKVKEVIGYNENQR"
     gene            14590..15639
                     /locus_tag="PKH42_03750"
     CDS             14590..15639
                     /locus_tag="PKH42_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOA19578.1"
                     /translation="MKIRDKEIKCYSKPYIIADIGANHNGDMELAKKMIDCLAECGAD
                     AAKFQSWNDKSLISREEYDKNQKYDDSPKKHFGSLKEMVEKYYLREEQHFMLKEYCVS
                     KGIEFCSTAFSEQEADLLDKLEVPFFKIASMDINNLRLLKYIAKKGKPVILSTGMATI
                     AEIETAVKSIESEGNKNIIILHCISIYPPNPKDINLNNIIMLRQLFPNYPIGFSDHTI
                     GVSVPMASVALGACVIEKHFTIDKNLPGWDHEISADSTEMKFIVQESKIVADSMGNYY
                     RTVSAAEEEKKKKFRRSIVLAKDIKKGEVIKNDDITYKRPGTHIRPDEDMYVIGRKLI
                     RDMHEDDLLDWCDME"
     gene            15684..16400
                     /locus_tag="PKH42_03755"
     CDS             15684..16400
                     /locus_tag="PKH42_03755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOA19579.1"
                     /translation="MIAIIPARGGSKGVPKKNIRELSGKPLIFYTINAAQKSRYISRI
                     IISTDSFEIAEVAKSFGCEIPFMRPPELATDTSKALDAYLYTVEKLNTEFNEQIRDFS
                     VLLPTSPLRNAEDIDKAIEIFYSKRAKTVISVVEAEHPPTWYKKINDKGILEDYFRGE
                     DNSLNRQEAEKTYLPNGAIYIFNYNSLKLNCNYYNDETYPYIMSRKSSVDIDTEVDFK
                     LAELIIRSSQLNYTHGDYNG"
     gene            16393..17715
                     /locus_tag="PKH42_03760"
     CDS             16393..17715
                     /locus_tag="PKH42_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HOA19580.1"
                     /translation="MDENFGRSMYEMADRLFPICRSLTGEGVRQTLSILKEVCPSMNV
                     YEVPTGTIAFDWKVPKEWNIKEAWIKDSNGNIVVDFKTNNLHVVGYSAPVNQKVSLNE
                     LMKYIYTDTSQPDIIPYVTSYYEEKFGFCMSDNHKNSLKEDTYHMVIDSELKDGSMTY
                     GEILIPSTNIDETDGSNKEVFLSTYICHPSMANNELSGPCLAIYLAKWIRELKNRRYN
                     YRIIFIPETIGSITYLSKNLDTMKKNIVAGFNISCVGDSNTYSYVASRHGNTLADRTA
                     KNILKYYYPEFKRYSFLDRGSDERQYNAPGIDLPVCGICRSKYGEYPQYHTSADNMNF
                     ISPEGLAGSFDVYVKCIEALENNYYYKTNFLCEPHLGKRGLYPATSRKGSVEKVRTMM
                     DFMAYADGEKDLIEISDIIDVPLYEILPIVEELMNKEVVIRVQKFVLR"
     gene            17753..18859
                     /locus_tag="PKH42_03765"
     CDS             17753..18859
                     /locus_tag="PKH42_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19581.1"
                     /translation="MISIFVCSTQFQLILSAMVSQEIRSKVSHRQVLLVDVYRDSLKT
                     FVRDNKEEIGKIWDEVYYINDNNSSNKSGILNNKLNGVKILFKVKKEIEEIFRKVKTT
                     EIKEIYIATGTYYSYVTKQLSRLGHQNNCIINIIEDGTSTYIYSKAKDNDYIIKESKK
                     IRNHINAVIKHILMYFLRYNNFDYDMADKIYVLNKDIYIGDESIKNKLIDIEVRQAIS
                     INLSMYPEDNIKDINRTDIIYFSQNIFDYEDEMIFLENLLSECNYCYKPHPSADNIIT
                     NNKILDSVPWEILCNYIEEDLIFISIASTAVFTPLLMYNKKNKAILLYKVLDINISKK
                     LDEFFIKVQNIYKDRVYVPEDSIQLQNVLKNIKK"
     gene            18930..20447
                     /locus_tag="PKH42_03770"
     CDS             18930..20447
                     /locus_tag="PKH42_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOA19582.1"
                     /translation="MSAKSIVLVNSFFYTFSALLVRAMSFFLLPIYTRFLTPEDYGIT
                     NLVNSFNSVAIFIISFSLYIAVVRFYTDYKDDREQIKRFYGTIIIFIFISGLAFCIIV
                     IIFRYILIRLIIKDISFYPVGFMALFILLFLSLNTVHQNILEGMQQGKKLTVINFIIF
                     GSQVCLNMLFIGIYKLGALGILLATLITNLGYSLFMLYDLKKNNLITLCIDIRILKES
                     LKYSIPIMPHNLSTPIANFISRLLINNSSSLSFVGLYSIASQFGSIIDTVQASVNQAF
                     APWFYDMMNSCNQEARKDVVNFSYSLILIYSFIYMVIGLFSQEAIIIMTNERYIIAWT
                     AVPILVIAYSVKSIYYFYVSVLFFYKEASRKLFIATISGSFADIIIAFVLVPKYGMYG
                     AAFSFLVAKVIVVTIVIIISKRYDDIGFSVIKMIIIIIPSLLFMIAGLYFSYTKYLTV
                     FSWINLLYKLVVLSAYLVYIFITNKKNISIIINSEFIKQIIGELKSKNFKELSLK"
     gene            20470..21831
                     /locus_tag="PKH42_03775"
     CDS             20470..21831
                     /locus_tag="PKH42_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19583.1"
                     /translation="MRTNKKIVYVCHCIDTEGPLYESLEATFERLETSIGIKIKPSQE
                     NLIKIQNQEMDLGGKEKVASLIVDKNLINYNDTWDKLDNMLDEMMSKPYRRKYADSFG
                     NGLIYNWFILDHIGYKTNPRRRDIGYHNIYNHYTVKIDEHDSYQDEINWHFHPMSAYM
                     EAHMCATSFINSPHLYESLARAVIDRCWFPSAFRAGFNTERPDSHWFLEQWIPFDFSN
                     QAIELTELDIQQMDNSDGRFGDWRRARADWAPYNPSHDDYQAEGSCRRTIFRCLNIGT
                     RIRLLNEDEVDKAFIRADCGTPTILAFTNHDFRDMRIDIENAHNLIMKTSKKYPDVRW
                     CNSGAKSAARKVLNIGNGDGINLTTRFDHKKDSVKITVETDSDSFGPQPFLAIKTFDN
                     KYMTDNFDFQIPRRKWTYTFDTNSINFKSVNKIGIATNDINGTTCVEVYDSTGEILQR
                     RKL"
BASE COUNT         8073 a         2934 c         4238 g         6692 t
ORIGIN      
        1 caaagtttaa ttcattttgg agaggatttc acctctgcag gacatcttta tttagctagg
       61 atacccaata ttagtgaaaa tgagagaaat gagattatta taaagatggc tgagaatggg
      121 atttcaacaa atgttcatta taaacctctg cctatgctta cagcatataa gaatttaggt
      181 tttgatataa aggattatcc gaattccttt gatatgtata agaacgaaat tacactgcct
      241 cttcatacat tgttaagtga tgaggatgtg aagtacattt gcgaaagttt gaagaaagca
      301 atgaataaag tgttgcaaac cagacaggtt gtggcagata agtaggagta agggtatgta
      361 taggaagtat tttaaaagaa taattgactt tgttctagct gtaatagctt tacccttttg
      421 gtttattata ttgattatta tcggagcaat tatatacatc caagatgaag gtccaatttt
      481 ctataatgca caacgactag gtcaagatgg aaagatattc aaaatgtata aattccgttc
      541 tatgaaggta aattctcttg atttcagaaa tgaagatggg tctactttta attctgagga
      601 tgatccaaga ttaactaaga taggtaagtt tatcagaaag acaagtcttg atgaaacacc
      661 ccagctttta aatatcatta aaggtgacat gagcattata gggccaagac ccgatttgcc
      721 agagcataga gatttatatg aaggtaatga ggaacgcaag ctagaagtta gaccaggggt
      781 ttcaggatta aatcaagctt attatagaaa tacagtacct tggaaagaaa gaattcagaa
      841 tgatatttat tatattgata atcttacaat attgttggat atcaagattt ttttaaaaac
      901 agtttcatcg gttcttaaga gggagtctgt atttatagaa cacaaatcta aaagcaagag
      961 tggtgtgaaa gctaatggct aataccaatt atgagtgcaa acatctgcag tgggatacgg
     1021 attattttgg tgttagttct gccagagtaa atctattcgg tattgtagat gaaaaaggtc
     1081 aagaagatat agtagagttt tgtaaaggtt atgattttgt tactatttct aatcttgaca
     1141 atacaaaaga aaataatcat tggataggca atagaactaa tgcatttctt actgatataa
     1201 atgttaagtt tattaaagtc ttggaggata agccagacta tcaagatgag aaaacctata
     1261 cagcgaattg cttatataaa aatgcacaga tagttgatat tgcaggaaag tcatttaatt
     1321 attccaggtt ttttaatgat tcaaaacttc cagaaaaaaa agcgaagaat atttatattc
     1381 actggactga atgcgccttt aataataaag ataagtactt tgttatctct gaaagagaag
     1441 gaaatgtagc aggctatatt ctgttttcat tcattgaaga cagaagtgtg attgaactca
     1501 tcgctgttga tgagaagtat caaggacaaa gagtcgggaa gtcactgatt aatacaatgg
     1561 aatcatttgt tatagataaa ggaattggaa aaattaaagc aggaacacaa gtgaataata
     1621 tttcagctac tcggttttat tcgaagatgg gcttcgagta tgtgagttgt ggaagtgtgt
     1681 atcatttgtg gagatgatag tatggagaca ggttaatgaa agtattatat attgcaacat
     1741 catttccgga acctaataaa ggtgcgacta tttatactga tttggctgaa gccctatatg
     1801 aagcaggtca tgaaataacg gtagcagttt cagaacaagc gaaaaaccta agccggacac
     1861 agatgaaaaa agaacgtgga tttgatgttt taagaatagt tactggaaat tattatgatg
     1921 taagtcttat agaaaaagga atcactacat taagaattcc aattataatg aaaaatgaaa
     1981 tctcaaagta catagtcaat agaaaatttg attttatact ttacgaagct ccacctgtaa
     2041 ctaatgctag cttagttgca tgggctaaaa agaagtttga ttgtccttca tatttaatgc
     2101 taaaagacat atttccacat aatgctgtcg atatagggat aatgaaaaag agcagtatta
     2161 tttatagata ttttataaaa aaagagaaag atctgtataa gtcagcagat attattggtt
     2221 gtatgtcaga agcaaacaag cagtatatta tcaagcataa ttcttggttg aatgagagta
     2281 agattgaatt gcttcctaat actaagagga ttactgatga gatcgaacct gtaaaactaa
     2341 agataagaca acaatactcc attccggagg atgcatgtgt gtttattttt ggcggcaaca
     2401 tagggaaacc gcaatatgta gacttgctga gccaagcaat tatagaatgt aaaaatgatg
     2461 aaggtatata tttcttattt gtcggaaggg gtacggaacg atacaaactg gaaaatacaa
     2521 taaaatcaga aaatataata aatgcaaaag ttattgaaaa cttaccgaga gatcaatatg
     2581 aacaaattac caaggaaagt gatgttggac tcataatttt agatcctcgg ttcacaattc
     2641 cgaattatcc atcaagaata ctatcttaca tggagtattc aaagccggta ttagcagcaa
     2701 cagataaaac aactgacata aaggatttga ttcttcaatc taaatgtggt gaatgggtat
     2761 ggtccggtga taaggataag tttgtagaag ctataagaag gatgtcaaaa agtccgttac
     2821 ttaaacagat gggaaggaac ggcagggatt atgttaaaaa gaatttttct ttagaaagct
     2881 ctgtagatat acttgaaaag cgttttaaca aagaaacgag gcagcaaaat gtttaaagat
     2941 aaaaccatgc taatcacagg cggaacaggc tccttcggca atgccgtaat gggaaaattc
     3001 ctgaaaacgg atataaaaga aattcgcatt ttctcacgtg atgaaaagaa acaggacgat
     3061 atgcgaagac actataagga tgacaagctt aagttttata taggagatgt aagggatatc
     3121 caatctgtaa aagatgctat gcacggagta gattatatat ttcatgccgc agcacttaag
     3181 caggttccgt catgtgaatt tttcccgtta gaagcagtaa aaacaaatgt tttagggaca
     3241 gataatgtgc ttacagctgc cattgaacat tttgtaaaga aagtcatctg tctctctact
     3301 gacaaagctg cttatccaat aaatgcaatg ggaatttcaa aagcaatgat ggaaaaagtt
     3361 tttattgcca agtctaagac agttgatgct gataagactc taatatgcgg aactcgttac
     3421 ggtaatgtga tggcttccag aggttcagtt atacccctgt ttattgatca gataaaaagc
     3481 ggacaaccat taactgttac agaccccaat atgaccagat ttttaatgag cttggaagaa
     3541 gcagtagagt tggtggtatt tgcatttcag aatgctgaag caggtgatat tatggttcag
     3601 aagtcgcctg catctacaat tgctgattta gctcaagctg ttaaagaact gtttaatgct
     3661 gataatgata taaaaattat cggcacccga cacggagaga aaagatatga aactctttta
     3721 acaaaagaag aatatataaa ggcggaagat atgggcagct tttatagagt gcctgctgat
     3781 aaaagagacc tgaattatga taaatatttc atagaaggaa gtcaaaagtt atcatctaac
     3841 ggagagtata attctgataa tacagaaagg ttgaatatag aacagattaa agaaaagtta
     3901 ctattacttg aatgtataag aaaagaattg gagtatttta attatagata tgaaacagca
     3961 atcaccatgt agtcttctaa attaatagaa aggccgaaat aagtgatgaa aatacttata
     4021 acaggagcaa agggttttat agggaagaac cttattactg aactaaaaaa cagaaaatat
     4081 acagatttat ttgaatacga tatagataca aaccctgttt tgcttgataa atattgtaaa
     4141 gaagcagaat ttgtatttca tttggcagga gtgaatcgtc caaaagagca atcggagtat
     4201 atggaaggaa attttgggtt tacatcaata ctgcttgatt tacttagaaa gcacaaaaat
     4261 atctgtccta taatgctttc ttcttctatt caagctgaat tagataatcc ttacggcaaa
     4321 agcaaaaaag cagtagaaga attattgatt gattacggga agaaaaacgg tgtaaaagta
     4381 ttaatctaca ggtttccaaa tgtattcggt aaatggtgca gaccaaatta caacagtgct
     4441 gtcgcaacat tctgccataa tgttgctcat gacctgccta taaccgtgaa cgacccaagt
     4501 gttgtaatga aacttgtata tatagatgat gtggtaaatg aattgataag tgcttatgaa
     4561 ggcaaagaaa atagaaagat cgaattctgc gaagttccta tagtctatac cattaccctc
     4621 ggagagattg ctaaattatt gtattcattc aaaaaaagca gagaagggcg ttcaattccg
     4681 aatatgtctg atgtatttat caaaaagctg tatagtacat atttgagtta tttaccggaa
     4741 gacaaattca gctatgaact aaagatgaat gtggataaca ggggttcttt tacagaattc
     4801 ataaaaacac cggacagagg acaggtttca gtaaatatat ctaaaccggg aattacaaaa
     4861 ggaaaccatt ggcatcacag caagaatgaa aaatttttag tagtcagcgg taaaggcgtt
     4921 atccgattca gaaaaatata ttcagatgaa gttatagagt attttgtaag tggagataag
     4981 atggaagcag tagacattcc gccgggatac acacataata tcgaaaacct tggtgatata
     5041 gatatggtta ccattatgtg ggtcgatgag tcttttgaac ctgaaaaacc agatacttgt
     5101 tacttggagg taacgatgtg aaaagattaa aagttatgac ggttgttggt acgagaccgg
     5161 agataataag actatctgct gttattaaca aattagaaga gtcaaaagtt atagaacatg
     5221 ttcttgttca tacaggacaa aactacgact atgaactata tgaagtgttt tttgaagatt
     5281 tcaaattaaa aaaacctgat tattttctta atgcagctaa cggaacagca gtagaaacta
     5341 taggtaatat tcttattaaa attgatccga ttcttgaaga aataaaaccg gatgcattct
     5401 tggtactcgg agatactaac agttgtttat gtgctatcgc agcaaagcga agacatatac
     5461 caatatttca tatggaagca ggaaatagat gttttgacca aagagtacct gaagaaacaa
     5521 acagaaaaat cgtagaccat atttcagata taaacttaac ttatagtgat atagcaagag
     5581 aatatctgtt acttgaaggg ttaccggctg acagaattat taaaaccggc agtccaatgt
     5641 ttgaagttct taattcaaga aagactgata ttgaaaaatc agatgtatta gagagattag
     5701 gacttgaaga aggaaagtat tttgtagtat cagcccatag agaagaaaat attaattctg
     5761 aagaaaattt tttggattta attgaaagtc taaatgaagt ggcagaaaaa tataaaatgc
     5821 ctgtgattat tagcactcat ccaagaacta gaaacatgat tttcacaaga ggtatcgaat
     5881 ttcatccact gatacagaca atgaaacctc ttgggtttaa tgattatatt aagcttcaga
     5941 ttaattccaa agctgtttta agtgacagcg gtacaatcag tgaagaatct tcaattcttg
     6001 gatttaaagc acttaacata agacaagcca atgaaagacc ggaagctatg gaagaaacat
     6061 cagtaatgat ggtaggacta aaaagagaaa gaattctaca gggcatagaa atattagaga
     6121 ctcaagagaa ggatacttta aggaaagttg gagattactg tatgcccaat gtttcagata
     6181 aggtacttag gataatacta tcttatactg attatgttaa tagggttgtt tgggggaaat
     6241 aacagaaaag aactggattt agaaaggtat gaaatttatg aaattgttat taatagcacc
     6301 atgtcctcct ccatatggag gaatatcaaa ctggagtaaa attgtaatcg atggaatcag
     6361 gaggtatttt gctgacattg accttgatgt aatagatata tctccacgaa gaagggaaac
     6421 tgaaggacga aaccttttta accgagctat ggatggtata actagtacta ttaaggcttt
     6481 atgtattgct aaaaaaacat taaaaaacag caaacctgat tctgttcatc ttacaacaag
     6541 cgggagcctg gcgttattaa gggactatgt gctgttaata tttttccgca agcatcaaat
     6601 aaatacagtt tatcatattc attttggcag aactgcagaa attattaaat atggcagttg
     6661 ggagcgcata ttgctaatgc atgctatgaa acatgcaggg tcagtaataa ttcttgatag
     6721 attaagcttt aattcactaa gagaacagtt tcccctgctt caagtaaaga agataaataa
     6781 tccaattaaa attatgggcg aaccccgcaa ttcaacatct aaatattata tgtttcttgg
     6841 atgggttttg aaaacaaagg gtatagaaga acttgtttca gcatggaatc aggcaggaat
     6901 aaacgcaact cttcgcattg tcgggcctta tcaagagtct tatttagagt atttacatca
     6961 ggccggctat gattttaaaa gcattatttt tgaaggagaa aaaggacatg atgaagctat
     7021 ggaaattctg tcagaatgtg aagcttttat tctgccaagt tattctgaag gatgcccata
     7081 tgttatttta gaggctatgt catttaaaaa accaataata gccaccaatg tcggtgatat
     7141 tccaaatatg cttagtaatg aatgcggaat ccttgttgaa ccgcatagta gcactcaaat
     7201 atgcgaggca ataaaaagtt tagaatctga ttcggatttg aaaattcgaa ttggttctaa
     7261 cgcatataaa agggtcttgg aagagtattc tatcaaagaa attctggact tatacagaaa
     7321 cgaatgggaa agcaaatatg aatctattcc atttttactg aatatttcat aaagttatat
     7381 aaattgctgt atgaggagat tatcttgaaa aaagttttga tagtaattcc aactgttaga
     7441 actggaggag cagaaaagtt agctttagat ataagccgtt tactagataa aaataaattt
     7501 gaagctacat tgctgtgcta ttatagtgac cagcccgatg cttacgaaag tcttataagg
     7561 gatgaaaata ttaagatcgt ttacctaaat aaaaaattag gacttgactt taccatgttt
     7621 tcaaaggtta aggaagtagt agaactgatt agtcctgata taattcacgc acatcttgac
     7681 acgttgctat atttgattcc ttcatttaat agaaaccagg tcaaactttt tacaatacat
     7741 agtaccccgt gctatgaggc gagtggaata ctccagaaga ttatccgcac tttttgtttt
     7801 cattataaaa atgttattcc tgtggcaatt agtcaatgtt ttgttcaaga cattgcctca
     7861 tattatcaat taaatgaaaa acgaattgca tgtataccaa atggcattga ttttgggaat
     7921 tttaatacaa gtaaacgcaa aaaaactgaa gataacatat ataaatttat tactgtagga
     7981 tcaatgagac ctgaaaaaaa tcagaaatta ctaataaaag catttgcata cgtacgtaaa
     8041 aaaaatccaa atacacaatt gacaattgtg ggtgatggaa ctctcaaacc ggaaattgaa
     8101 aaacttatag ctgcatacca ccttcaagat gctgtaaatt taacaggaat agtcagtaat
     8161 gtgggggatt atttagctag tgccgatgct tttgttcttt catctgattt tgaaggtctt
     8221 ccattgtctt tattggaagc aatggcatgc ggtctcccga ttgtttctac aaaatgcggt
     8281 ggtacagttg acattattga tgatggagtg aatggatatt tagtggatat aaatgatatt
     8341 gggcagctga gtgatgccat gtatagagtg acactacttt ctgaagagtt aagactacgt
     8401 atgattgaaa aatcatttga gattttgcct atgtatgata ttaaacgctg cactggcgat
     8461 tatgagagat tatatacaaa aaatatagta actatgctta aataaatgaa ttgcgagggt
     8521 accatgaatg atacaatagc aacttgcaaa tcaaaacatc caattagcgt gcgatgcctt
     8581 tttgcctggc ttgtactcat gccattttca tttgttgcac ttggcagtct tgggtctata
     8641 ttgaagttgc tgacgattgt aattattgta tactatttta taaaaggcgg acgattaaaa
     8701 tatcgaaaat cagcaatttt atttacatgg tttttatatg ttatatatta tgcagtcagc
     8761 ttaatatggt cattgaattt atcggatgca atagtatatg taattggtta tattcagttt
     8821 tttgttttag cttttctttt tagcagcgag attatggata atgacgatat taggcatttg
     8881 gaagatgcat ggatatttat atcgatattt tgtattatca tttttataac cgggggagac
     8941 aaatcaattg aatatagtga taggagtaca ttgtatatgt taggtacatt taccgatcca
     9001 aatgaattcg ggggatattt ctgtattccg actggactga tgtgtagcag gttgttttct
     9061 gaaaacaagc gtaaaagttg tatttatttg attcttatta ttgtttcaac atatattgtg
     9121 ttgaggaccg gttccagggg tgcacttctg gctttaatag tatgcattat agcagccatg
     9181 tttcgagctg ccagaatgtc atttaaaaac actcttttta taatagtgct tgccattttg
     9241 tcatatttta ttattctcaa ttttatgcta cctcaaattt ctgaatcagt tctcaatcga
     9301 tttacatttg aatcattaaa agctgatggt ggaagtgaaa gaaaattaat ttggacaaaa
     9361 tctattaagt atttttatga agaatcaata tttaggcagg ttttgggctt aggcatattt
     9421 ggtggaactt tttataaagc aacgatgcat aatcagttat tacaaaatct gattgatggc
     9481 ggatttatag gcttgttatt atattgtgta cttatggtat tgggatttat aaatatatat
     9541 aaaaaataca agaattttgt acccggttat atcggaatga tgctgttaag catgacgctg
     9601 acaatgactt catcgtataa accactatgg acattgatga tattaggatt aattgacata
     9661 agaagagaat aacctaagct taaatatttt taccttgctt tttatttcga cacatctcaa
     9721 attttataag gcttacaaaa ataatcgcct aaaataatat ttacattatg gaaaaatcta
     9781 aggtggacaa gtttataaag gaagaattga aggtaataaa atggaggatg gctatgatga
     9841 aagttttagt tacgggaggg gcaggctata taggcgctgt cttagtaagg aaattgctga
     9901 aagaaggtta tgaggtaaga gttcttgatt cgcttaagta tgggggagaa tcaattatag
     9961 atttgctaaa taaccctaag tttgaatttg tcaaaggtga tatcagagac agggaaattg
    10021 catatgcatc aaccaagaat gtggatgcag tagtgcatct tgcagcaata gtaggcgacc
    10081 ctgcttgttc acaatttcct gttgaagcaa aggaagtaaa ttatgatgca tcggcgcaat
    10141 tatatgaaga atccttgaaa aacaatgtag ggaggttcat tttttcttcg acttgctcta
    10201 attacggaaa gatggataat tctgaaaaat tcttgaatga agaatctgaa ctaaaaccat
    10261 tgtctcttta tgctcaaaca aaggttgaat ttgaagaata cttgctcaac caagataaaa
    10321 ttaatgtttg caagcctgta atactgaggt tttcaacagt ttacgggata tcaccacgca
    10381 ttagatttga tttaactgta aatgaattta caaaagaact tgccatgggc agggagcttt
    10441 tggtatatgg agaacagttt tggcgcccct actgtcatgt ggatgatttg gccaactcga
    10501 ttttgtttgt attagaaagt gatgaggcaa aggttgaatt caatgttttt aatgtgggca
    10561 gctcagatga aaactatcaa aagaaaatga taataaatga aattttaaaa caagtacccg
    10621 atggtatggt taaatatgta acagttaatg aggacccaag agactatcga gttaattttg
    10681 ataaaatcag agatgtgcta ggatttaaag taaccaaaag agtacctgat gggataaatg
    10741 aaatagttac attgatagag aatggtttca taattaaccc agatgatgat aagtattaca
    10801 acattataag agtaagaaaa ccagctatat tggaggttta gtatggcaca aaggtttatt
    10861 cccctttctg ttccaaattt aaagggcaga gaattgcagt atgttcagga tgctattgaa
    10921 actgaatggg tttccacagc aggaaaatat gtaaataaat ttgaagagaa aataatatct
    10981 tacgctatta ctaagggagc agtttcttgc cagaacggaa cagcaggtct tcatacagcc
    11041 cttatcgtat ctggtgttac aaaagatgat gaagtaattg tacctgcatt gacattcata
    11101 gcatctgtta atccggtcaa gtacattggt gcagaacctg ttttcatgga ttgtgatgat
    11161 tcattgaata tggatactga taaactggag gatttttgca gaagggagtg catatttgca
    11221 gacggaagtc ttattaataa acagactaaa aagcatataa aagcaataat aattgtccat
    11281 gtattcggaa atatggctga catggaaagt ataattgaga tagcaagaga atacaattta
    11341 atagtaattg aagatgctgc ggaagcaata ggaacatatt atactcaggg caagtatgaa
    11401 ggcaggcatg caggcactat aggagatgca ggtgtctatt catttaacgg caataaaatc
    11461 attacgacag gcggtggtgg aatgatagta tcaaatgatg aggacctgct aaaaagagca
    11521 aagcacctaa ctactcaagc taaaacagac gaactgtact atacccatga cgatgtggga
    11581 tacaactatc ggatgacgaa tgtacaggca gcattgggac tggcacaatt agaacagtta
    11641 gaaacattta ttaaaataaa aattgaaaat tatattttat ataaagaaaa gattcatgag
    11701 atacccggtc tttctttatt gaactttaat gaggatacca gaagcaacca ttggctttat
    11761 tccttaatat gcggggatga atatccgttg aacagggatg aaataataat gtatttatct
    11821 tcaaagggtg tacaagcaag accaatatgg ggattgataa atgaacaaat tccatattta
    11881 aaaaatcagg catataagat tgagaaagca aaattttatg tagaccatgt ggtaaatata
    11941 ccgtgcagca caaatctgac aaaagacgag atcctatatg tagtagagaa tctgagcaat
    12001 gcagcactga aatgaaagtg gtgatactat ggaaattaag gatatcctca tagatgaaaa
    12061 ctcaacaatg attgaagcta tgaatgccct tgataaaacc gcaaaaaaaa tactttttgt
    12121 tgttaaagaa gacaggctgg tggctgctat cactgatggc gatataaggc ggtggatatt
    12181 agctaagggc agtcttgatg caagggttaa aagtattgca aattataaac ccttattttt
    12241 gtatgaaaag gataaagctt tagcaaggaa atatatgaaa caaaacttaa ttgatgcttt
    12301 acccattgtt gatgaaaata aaaagattat tacagtaaaa tttttatata ggcaggacat
    12361 taagaacaag gttgaactgg attgtcctgt agttatcatg gcagggggac ttggatccag
    12421 gctgtatcca tatacaaaga tacttccaaa acccttaata ccaataggag aaattccaat
    12481 tgcggagcat attattaaca ggttccagca actaggcata aacaactttt atatgattgt
    12541 aaatcacaaa aaaaacatga taaaaaccta ctttaatgaa atagcaaaga attacaagat
    12601 aatttatata gatgaaaatc aacccttggg gacaggagga ggattgagtt tattaaaggg
    12661 tattgtaaag gaaactttta tactaacaaa ctgcgatata attattgagg aagatattgg
    12721 gaaaatttat aattttcaca aagaaaaaaa taatttaatt actatggtct gttctttgaa
    12781 aaatattact attccatatg gcgttatcaa cataagtgag gacggccgaa tagaggatat
    12841 gaaggaaaag cctgtactta agttttttac aaacaccggt atgtatgtgg ttgagtcgga
    12901 cattgttaat gaactggaga ataataaaac tatagatttc cctgatataa ttgaacaata
    12961 taaaaaaagc ggaggtaaaa taggtatata tcctataagt gagtattcgt ggatggatat
    13021 gggacaaata gatgtactag aaaatatgag aaggcgattg gaaaatgaaa tgtaaaaaat
    13081 tgctgttaat aggtggagga ggacattgta agtctgtatt ggattcaata ttagacaata
    13141 atgattatat tgacatagga ataattgaaa aaaaagaaat ttgttatggc gatatattgg
    13201 ggattagcat aattggatgt gatagtgagc ttgaatatta ttacaacaac ggttatcaat
    13261 ttgcttttgt aaccgttggc aacataggaa atccaaaaat tagaattaag ctttataaac
    13321 ttgcatataa aattggtttt aaaattccca atataattga ttcttccgcc tgtgtcagca
    13381 ggtttacaaa tcttggcaga ggaattttta taggtaaaca ggccgttgta aatgcaggta
    13441 cttcaatcgg agattgtgca ataatcaata caagctcggt tattgaacat gattgtatta
    13501 ttgaagaatt tgtcaatgta gcaccaggtg ctgttgtatg cggcaatgta acagtgggga
    13561 gaaattctta tataggtgca aaaagtgtta taagacagca gttgaaaatc ggttctaata
    13621 caataattgg catgggtagc actgtaacaa agaacataga cagtaatgtc ttagcctatg
    13681 gaaatccatg cagggttata aatagcaaat actaaagaag gttgaaagtg aaaacagcac
    13741 tgggaaccgt tttatataaa caatcattta aatatttaaa tgagttttct aggtcagtaa
    13801 acaatcaaga ctgcccggat tttgatatgt tgtttttaaa tgacgattta gatgaagccg
    13861 aaatagatat ttccataaat tcaataaaaa agaaagtaat attatcatca ggaaaaccaa
    13921 atagtaccat accccagtta agataccagc ttatcaagta tgcaaaagaa aataattatg
    13981 atttgctggc gttggtagat tttgacgaca cattctcaga aaacagggtt tcaaaagtca
    14041 aaacagcatt caataatgaa tacggctttt attacaacga tatttattat ttttataaaa
    14101 agaataaatt ctttagtttt ttgcccgact atacaaatga agtcaatcaa atacttgagc
    14161 aaaattattt gggtttaagc aatacagcaa taaacttaag taagattgat tttgatttgc
    14221 tggaaaaatt aaatgttaca atatgtaaag cctttgactg gtatatgtat tcagtttttc
    14281 ttgcagaggg ccataaaggg aaaaaagttg acggttgcag aacgtattac agaatttatg
    14341 aaaataacac tgccggtgaa tgtaaaaaca caataaacta tatcaataat gaaattaaca
    14401 caaaaataaa ccactattca ttattaaaac agtgggatag caagtatgaa gaactttatg
    14461 attattacaa agcattgcag catgaatgga aaagaggaaa tgttgattta ttaagctata
    14521 ttgatatcaa aaatgattat tggtggggtc ttataaaatc aaataaggtg aaggaggtta
    14581 taggttataa tgaaaatcag agataaagaa ataaaatgtt actcaaaacc ttatattatt
    14641 gcagatatag gagctaatca caacggagac atggagcttg caaaaaaaat gattgattgt
    14701 cttgcagaat gcggggcaga tgccgcaaag tttcagtcat ggaatgataa atccttaata
    14761 tcaagggaag agtatgataa aaatcaaaaa tatgatgact ctccaaagaa acatttcgga
    14821 tcattaaaag aaatggtaga aaaatattat ttaagagaag aacaacattt tatgcttaaa
    14881 gaatattgcg tttcaaaagg aattgaattt tgctctacag cattttcaga acaggaagcg
    14941 gatttattgg acaagttgga agtacccttc ttcaaaatag catccatgga tattaataat
    15001 ttaagattat taaagtatat tgcaaaaaaa ggcaagccgg taattttatc tacaggcatg
    15061 gctactatag cagaaattga aacagcagtt aaatcaattg aatcagaggg aaacaaaaat
    15121 ataattatat tacactgcat ttccatatat ccgccaaatc caaaggatat taatttaaat
    15181 aatattatta tgcttaggca attattcccc aattatccca ttgggttttc agatcatact
    15241 ataggggttt cagttccaat ggcttctgtt gctttgggag cttgtgtaat agaaaagcat
    15301 tttacaatag ataaaaattt acctggttgg gaccacgaaa tttctgctga ttcaacagaa
    15361 atgaagttta ttgtgcaaga aagtaaaatt gttgctgact ctatgggtaa ttactataga
    15421 actgtttcgg cagcggaaga agaaaaaaag aaaaaattta gaagaagtat agttttggct
    15481 aaggatatta aaaaaggcga agttataaaa aatgatgata taacttataa aagacctggt
    15541 acacacataa ggcccgatga ggatatgtat gtaattggaa gaaaattaat aagagatatg
    15601 catgaggatg atttattgga ctggtgcgat atggagtaat ttgtagtgag gttatatctt
    15661 ggcaattaaa ggagcaaaaa aatatgatag ctattatacc ggcaagaggc ggatcaaaag
    15721 gtgttccgaa aaagaatata agagagctgt caggaaaacc tttgattttt tatactatta
    15781 atgcagctca aaaatcgaga tatatcagca gaattataat ttctaccgat agctttgaaa
    15841 tagcggaggt tgccaaatct ttcgggtgtg aaataccatt tatgagacct cctgagctgg
    15901 caacagatac atcaaaagcc ttggatgctt atttatatac agttgagaaa cttaatactg
    15961 agtttaatga acaaataagg gatttctcag tactattgcc cacatctccc ttaagaaatg
    16021 cagaagatat tgataaagcc atagaaattt tttattcaaa aagggcaaaa acagtgattt
    16081 ctgttgttga agcagaacat cctccaactt ggtataaaaa aattaatgat aaaggtatat
    16141 tggaggatta tttcagggga gaagataaca gcttgaacag gcaggaagct gaaaaaactt
    16201 acctgccaaa tggtgcaata tatattttta attataactc gttaaaatta aattgtaact
    16261 actacaatga tgagacatac ccttacataa tgagcagaaa aagctcagtg gatattgata
    16321 cagaagtaga ttttaaactt gctgaactaa ttataaggag cagtcagttg aactacacac
    16381 atggagatta taatggatga gaattttgga agatcaatgt atgaaatggc agataggctt
    16441 tttcccatat gcagaagttt aacaggtgaa ggtgtgagac aaactcttag tatattaaaa
    16501 gaagtttgtc cttcaatgaa tgtatatgag gtacctactg gaacaattgc attcgactgg
    16561 aaggttccaa aggaatggaa tataaaagag gcttggataa aagattcaaa tggcaatatt
    16621 gttgttgatt ttaaaacaaa taatctgcat gttgtaggtt actcagcacc ggtaaatcaa
    16681 aaggttagtt tgaatgaatt aatgaagtat atttataccg acacgtctca gcctgatata
    16741 attccctatg ttacttcata ttatgaagaa aaatttggct tttgcatgtc ggataatcat
    16801 aaaaacagtt taaaagaaga cacgtaccac atggttatag atagcgaatt gaaagacgga
    16861 agcatgactt acggcgagat attgattcca tcaacaaata tagatgaaac tgatggcagc
    16921 aataaagaag tatttctgtc cacctatata tgtcatccgt caatggcaaa caatgaatta
    16981 tcaggccctt gtttggcaat atacctggca aaatggatac gggaattaaa aaacagacga
    17041 tataattaca ggattatatt tatacctgaa acaataggct caattacata tttaagcaaa
    17101 aatttagaca ccatgaaaaa aaatatagtt gcaggattta atatttcatg tgttggtgac
    17161 agtaatacat attcatatgt tgcatctcga cacggcaata ctttggcaga cagaactgca
    17221 aaaaatatat taaaatatta ttatcctgaa ttcaagagat actccttttt ggacagaggt
    17281 tccgatgaaa ggcagtataa tgcaccgggt attgatttgc ctgtatgtgg catatgccgt
    17341 tcaaaatacg gagaataccc gcagtaccac acatctgccg acaacatgaa tttcatttcg
    17401 ccggagggct tggcagggtc atttgatgtt tatgttaagt gcattgaggc cttggaaaac
    17461 aattattatt acaaaacgaa ctttttatgt gagcctcatc taggcaaaag aggtctttat
    17521 cctgcaacca gcagaaaggg atcagttgaa aaagtacgaa caatgatgga ttttatggca
    17581 tatgcagatg gggaaaaaga tttgatagaa atttctgata ttattgacgt tccattatat
    17641 gaaattttgc ctattgtgga agaacttatg aacaaagagg ttgttattcg tgtgcaaaaa
    17701 tttgtactaa gataaataga tgcatagatg aataatgaaa ggaagtaaaa ttatgattag
    17761 catatttgtt tgcagcacgc aatttcagct tattttgtca gctatggtat cacaggaaat
    17821 aagaagcaaa gtgagtcata ggcaggttct tttagtagat gtatacagag attcacttaa
    17881 aacatttgta agggataata aagaagaaat aggcaagata tgggatgaag tttactatat
    17941 aaatgataac aattcaagta ataaaagtgg gattttaaat aacaaattaa atggagtaaa
    18001 aatattattc aaagtaaaaa aagaaattga agaaattttc agaaaagtaa aaacaactga
    18061 aataaaagaa atctatattg caacgggtac atattacagc tatgttacca aacaattatc
    18121 acggttagga catcaaaata attgtataat aaatattatt gaagatggaa ccagcacata
    18181 catatattca aaagcaaagg acaatgatta cataattaag gaatcaaaaa aaataagaaa
    18241 tcatattaat gctgtaatta agcatatttt gatgtatttt cttaggtata ataattttga
    18301 ttatgatatg gctgataaaa tatatgtact caataaagat atctatatcg gagacgaaag
    18361 cattaaaaac aagttgatag atatagaagt ccgtcaagcc ataagcatta acttaagcat
    18421 gtaccccgag gataacataa aagatattaa tagaacagat attatatatt ttagccagaa
    18481 tatatttgat tacgaagacg aaatgatatt tttagaaaat ctgttaagtg aatgtaatta
    18541 ttgttataag ccacacccca gtgcagataa tatcataaca aacaataaaa tattggattc
    18601 tgttccatgg gaaatattat gcaattacat cgaagaggat ttaattttta tatctatagc
    18661 gtcaactgct gtctttacac ctcttttgat gtataacaaa aaaaacaagg caattttact
    18721 ttataaagtt ttggatatta atataagtaa gaagttagat gaatttttta ttaaggtgca
    18781 aaacatctat aaggatagag tctatgtgcc ggaagattca atacaattac aaaatgtttt
    18841 aaaaaatata aaaaaatgaa ttattaaata cagtgagttt taaagagagt tacgcttttg
    18901 aattatgaat aaggagaaat cacattataa tgagtgcaaa aagtatagtt ttagtaaatt
    18961 ccttttttta tacatttagt gcattgttag tgagggcaat gagttttttt ttactgccaa
    19021 tatacactcg attcctaacg ccagaagatt atgggataac aaatctcgtt aatagcttta
    19081 attcagtagc aatttttatc atttcgtttt cattgtatat tgcggtagtc agattctata
    19141 ctgattataa ggatgataga gaacaaataa aacgtttcta tggaacaata attatattca
    19201 tatttatatc cggattagca ttttgtatta tagtaataat ttttagatat atattaatta
    19261 gacttattat taaagatatt tccttttatc ctgtgggttt tatggcatta tttatcctgt
    19321 tatttctttc actgaataca gtacatcaaa atattttgga aggaatgcaa caaggtaaaa
    19381 agttgacagt aataaatttt ataatttttg gaagtcaagt atgcctaaac atgttattta
    19441 taggtattta taaactaggt gcactaggta ttttactcgc aacattaata acaaatttag
    19501 gttattcttt atttatgtta tatgatttga aaaaaaataa tttaattacg ctatgcattg
    19561 atataaggat attaaaagaa tcattaaaat attcaatacc tattatgcct cacaatttat
    19621 caactcctat tgcaaacttt atttcacgtt tacttattaa taatagcagt tcattatcat
    19681 ttgttggtct ttatagtatt gcatcacaat ttggttcgat tattgatacg gttcaggctt
    19741 ctgtaaatca ggcttttgcc ccatggtttt acgatatgat gaatagctgt aatcaagaag
    19801 ctagaaaaga tgttgtaaac ttttcttatt ctttaattct catatattcg tttatataca
    19861 tggtaattgg tcttttttct caagaagcta tcattataat gactaatgag agatacataa
    19921 tcgcttggac agctgtaccc attttagtaa tagcctattc agttaaatct atttactact
    19981 tttatgtaag cgtattattt ttttacaaag aagcatcaag aaaactcttt atagcaacta
    20041 tcagtggtag ttttgctgac attataattg catttgtatt agttcctaaa tatggaatgt
    20101 atggagcagc tttttctttt ttagttgcaa aagtaattgt tgtaactata gtgattatta
    20161 taagtaaaag atatgatgat attgggttta gtgttataaa aatgataata atcattatac
    20221 ctagtttatt gtttatgata gctggactct attttagtta tacaaaatat ttgacagtgt
    20281 ttagttggat aaacttgctt tataaactag tagtattatc agcttattta gtttatattt
    20341 ttataacaaa taaaaaaaat atcagtatta taattaactc agaatttatc aaacaaatca
    20401 taggagaact aaagagcaaa aattttaaag aattatcatt gaaataaaaa tatattaatg
    20461 aggtgaataa tgagaaccaa caaaaaaatt gtatatgtgt gtcattgtat cgatactgaa
    20521 ggacctctgt atgaatcctt ggaagctact tttgagaggc ttgaaacaag tattggcatt
    20581 aaaataaaac ccagccaaga aaacttaatt aaaatccaaa accaggagat ggatttaggc
    20641 gggaaagaaa aggtggcaag tttaattgtg gataagaatc taataaatta caatgacact
    20701 tgggacaagc tggataatat gctggatgaa atgatgagta agccatacag gaggaaatat
    20761 gcagattcat ttggtaatgg attgatatac aattggttca tacttgacca tataggttac
    20821 aagactaatc ccagaagaag agatataggc taccataata tttacaatca ttatacagta
    20881 aaaatagacg agcatgattc atatcaggat gaaattaact ggcacttcca tcctatgtct
    20941 gcatatatgg aagcccatat gtgtgccact tcatttatta attctcctca cttgtatgag
    21001 tccctagctc gtgcagtcat agaccgctgt tggtttcctt cagccttcag agctggcttc
    21061 aacacagaaa gaccggacag ccattggttt ttagaacaat ggataccttt tgatttttca
    21121 aatcaggcta ttgaattaac agagctggat atacagcaaa tggacaattc ggacggccgg
    21181 tttggagact ggcgtcgagc aagggcagat tgggctccct acaatccgtc acatgatgat
    21241 tatcaagctg aaggctcatg cagacgaaca attttccgat gtcttaacat cggaacaagg
    21301 attcgcttat taaatgagga tgaggtggat aaggcgttta taagagctga ttgtggtaca
    21361 cctacaattt tggcatttac caaccatgat ttcagagata tgagaattga tatagaaaat
    21421 gctcataacc taataatgaa aacttcaaag aagtacccgg atgtaagatg gtgcaactca
    21481 ggagcaaaat cagcagcaag aaaagtacta aacataggta atggtgatgg cattaattta
    21541 accacaaggt ttgatcataa gaaggattca gttaaaatca cagttgaaac tgactcggat
    21601 tcctttggac cgcagccatt tttggcaata aagacatttg ataataaata tatgacagat
    21661 aattttgact ttcaaatacc aaggcgcaaa tggacatata cttttgatac aaattccata
    21721 aacttcaaaa gtgttaataa aataggcatt gcgacaaatg atataaacgg gaccacatgc
    21781 gttgaagtgt atgattcaac cggagaaatt cttcaaagga gaaaactata aaaaatgata
    21841 ctataatatc agaggagggt tatagttgta tattatgatt ctaaaacaat cttcaaataa
    21901 aaagcaaatg atatgccatt attatattct ccaaagt
//
LOCUS       DAOPUE010000025        21634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2418_length_21634_cov_10.035915, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000025 DAOPUE010000000
VERSION     DAOPUE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21634
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_21634_cov_10.035915"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PKH42_03780"
     CDS             <1..506
                     /locus_tag="PKH42_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOA19584.1"
                     /translation="IELLIGFTGGIATTYVHQHLKEGDTAHINGPYGDFYYHDDDGPI
                     ILAGAGTGMAPIMSILQYMADNNIMRETIFFFGARTMDDLFLVDRIKGFEEKLNLKFV
                     PTLSREESPEWKGEKGRVPNAIDKYVENAENYSAYLCGSPAMIDSIVEALVKKNVPVE
                     KIYFDKF"
     gene            complement(713..1216)
                     /locus_tag="PKH42_03785"
     CDS             complement(713..1216)
                     /locus_tag="PKH42_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19585.1"
                     /translation="MFSDKFVITSSGGLPQELSQEEFFDGISAPRNKELMRVFKDVKL
                     VEQLGSGVQRILKVYNKNVFKFSPNFLKVSFPIKNVGENVGENVGENVGENVGETMIK
                     INNTQKSIISIIKSDKHITQAEISKKLNKTIRTVERNMKKLQEADIIVRVGSDTLGYW
                     EVVVKEV"
     gene            complement(1769..2935)
                     /locus_tag="PKH42_03790"
     CDS             complement(1769..2935)
                     /locus_tag="PKH42_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOA19586.1"
                     /translation="MRIAVAGTGYVGLSVAVLLAQHNEVIALNRTQEKADMINNKKSP
                     IVDAEIQDYLTNRSLNLRATTDSNTAYKNAEYVIIATPTNYDENKNYFDTSSIEEVIT
                     NVLRINPEAVMVIKSTVPVGYTEKIKEIFDTDNIIFSPEFLREGKALYDNLYPSRIVI
                     GEKSERAEIFANLLAEGAIKKDIPILYTDATEAEAIKLFANTYLALRVSFFNELDTYS
                     EVRGLNTQQIIEGVCSDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYTDVPQNIMTAV
                     VDANRTRKDHIADMIIKRNPKIVGIYRLTMKTDSDNFRQSSIQGVMKRIKGKGIEVVV
                     YEPVLKDDSFYNSRVIRDLDEFKRISDVIVANRMSNDIRDIEDKVYTRDLYCRD"
     gene            complement(2957..3976)
                     /locus_tag="PKH42_03795"
     CDS             complement(2957..3976)
                     /locus_tag="PKH42_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOA19587.1"
                     /translation="MKLLITGAAGFIGFHLTRKLLDQSYEVVGIDNLNDYYDPSLKNS
                     RLEILGEYNNFNFQKVDLKDKASIDIIFETYRPTHVINVAAQAGVRYSIENPYAYVDS
                     NLLGFINILEACRNYPVDHLLYASSSSVYGGNNVVPFSTNHNVDHPVSLYAATKKSNE
                     LMAHTYSHLYGIPTTGLRFFTVYGPWGRPDMAYFSFTKDILAGKPIKVFNHGKMERDF
                     TYIDDIVEGIVKLIGKAPVPNKEWDESKDDLSTSFAPYKIYNLGNNNPVKLMRFINAL
                     ESALGKEAEKVYMDMQPGDVLRTYADVSDLEKDINFKPSTSIEDGLRKFVNWYMDYYK
                     IRDND"
     gene            complement(4295..5281)
                     /locus_tag="PKH42_03800"
     CDS             complement(4295..5281)
                     /locus_tag="PKH42_03800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA19588.1"
                     /translation="MLNREDNKVSVIVPVYKIEKYLTKNIESIIKQNIDMEIIYVDDG
                     SKDKSVEIIKKYMHLDQRIKLLQKDNGGCASARNYGLKNATGKYVIFIDGDDYINENS
                     LEALYNAAEQENLDIVQGSYRLVDDKGSIVIRKCNDGRGNIGDCLSGFQWLVRKNFNF
                     VVWLYLFRREYLIDNNLWFSEDIYHEDFEFIPRALYFAKRIKAIDLCFYNYVQRQGSF
                     MHTKNIKKCTDLVKIGDRYKDFANDNIDKKTQADVYDFFEKHLRYAYVSSLNSAIEQG
                     IPLSVLLEDKQLKNKLINEISKSGKKRHQLISYLLKYNLYNTYKYLFLIRNL"
     gene            complement(5268..6215)
                     /locus_tag="PKH42_03805"
     CDS             complement(5268..6215)
                     /locus_tag="PKH42_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOA19589.1"
                     /translation="MAKRIVVTGGAGFLGSHLCERLLIERNDVICVDNLFTGSKENIR
                     HLFNNNYFEFIRHDIVTPLYVECDEIYNLACPASPVHYSHDPIKTAKTSIFGSVNMLG
                     LAKRTNSKILQASTSEVYGDPQVHPQKEDYWGNVNPLGLRSCYDEGKRAAETFFMDYY
                     RKYNVSVKIARIFNTYGPKMSFNDGRVVSNFIIQALKNEDITIYGDGSQTRSFCFVDD
                     LIEALIKMMDTESNFTGPVNIGNPEEHNILELAEAIIRLTSSKSKIVFQKLPKDDPMR
                     RKPDISLARESLKWEPKVSLNDGLTKTIKYYGEWLNVKS"
     gene            complement(6245..7807)
                     /gene="murJ"
                     /locus_tag="PKH42_03810"
     CDS             complement(6245..7807)
                     /gene="murJ"
                     /locus_tag="PKH42_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOA19590.1"
                     /translation="MSTIKTTAIIITMISLGSKLLGFAREIVLAYFYGTSYVVDAYLM
                     AIAIPGIVFGWIVSLSVSYTPIYTEVRVKSGEDNAKRFNNNIISIAITIAIICAFIGV
                     LFNNQIVSLAAPGFEGEVYNLTNRFLKVTVFSISINVFVQILISYLNCHNKFIASNIS
                     TLIISSTQLLVVYISSKLGKEILIYGTILSYIAQLSLLLFFSYKNGYVYKYELKITPE
                     IKQAFSILVPIFISSMTIQINSFVNKAFASNLAEGSISALNYSGVIRYFIFYIFTVAL
                     TTMIYPMLSSSMAKNDINTIKRLVSKAITIIIILFIPISVGAIILSEPAIFFVYQRGE
                     FSSQSTLMTSAALQMYSIGLAAVALRDVITKVFYSMKETKAIMYISFLTVFACIIFST
                     ILIKPLEHAGLALAASLSDIITLPLFFYFLRIRLGNLGLKDSLTILIKSCISSVVMGL
                     LVYFTFNYLNSSFEPGKIITLLSIAASAAAGASIYFVLMLLMKVEEMNYFTDLVKYFN
                     KKVFNLINKRGQ"
     gene            complement(7864..9033)
                     /locus_tag="PKH42_03815"
     CDS             complement(7864..9033)
                     /locus_tag="PKH42_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA19591.1"
                     /translation="MKILHLCLASFYIDNYSYQENMLPKFHKDMGLDVEIIASLVSFD
                     KDGNPNLLNESGSYINEYGIPVTRLNYSKKKYSKTLRTYTNTYETICKTQPDIIFAHG
                     CQFLDIKHVVKYVKLHPNTRIYVDNHADFSNSARNFLSKNILHKIIWKYCAHQIEPYT
                     KKFYGVIPTRVDFLRDVYKLPKEKIELLVMGADDEKVTEAKDPDIKHEVRNKYNIKNE
                     DLLIITGGKIDKEKRQILDLMEAVQKIDYDNLKLIVFGSVIKELKDEINKLADGVKVQ
                     YVGWIKSSESYKYFAASDLVVFPGRHSVLWEQAAGLGIPLIVRHWEGTNHVDLGGNCK
                     FLYGNSVDEIYKLLMSLINNKDELDHMREIAESEGMKFFSYKDISCRAIEFKEQQ"
     gene            complement(9053..9655)
                     /locus_tag="PKH42_03820"
     CDS             complement(9053..9655)
                     /locus_tag="PKH42_03820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HOA19592.1"
                     /translation="MNLSFLISKLIKKLHIPAIKNSTIDKTAKVCSGSHIVNSTLGRY
                     TYITNFCSVFNCDIGNFCSIADNCIIGGHRHPIDWVSTSPVFHEGKNIMRKNFSEHEF
                     ETGQKTIIGNDVWIGNNCLIKGGIKIGNGAIIGMGSVVTKDVGDYEIWAGNPAKLIRK
                     RFDDETIDILIKSNWWNMDDAALQAAALNFNNIEKFKIPH"
     gene            complement(9684..10730)
                     /locus_tag="PKH42_03825"
     CDS             complement(9684..10730)
                     /locus_tag="PKH42_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOA19593.1"
                     /translation="MNKNEKEVQYYVYQTDLKEIFLVLWKRKKMIISLSLIVGLIVAL
                     YSKFVIIPTYETKLNIIISMPETYTTRYGEYELPFFDNSQYLNLIKSNDVIANTIKYM
                     DYDNDVSIEDLRGKVTILETKAASGVKQNSFEIIVSERTPEESLKLAQNLYKSYLDFI
                     DVMIKEVAINNYYNEFTLQNKNLEKQLNSNKEILKRNEELLNEIPKSLSQEGSSIDIK
                     RSLNESIDYVIPINAINPNYISIESDIIQNKQSINEIENHIRMNNMFLTELEEELQSV
                     KEYYETGKVKKLESNIVSSVTANIYLPSPPVAPTFKSSPSIALNTAIGLSIGLFIGVL
                     ITLIKEYWLDVNKK"
     gene            complement(10765..11961)
                     /locus_tag="PKH42_03830"
     CDS             complement(10765..11961)
                     /locus_tag="PKH42_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA19594.1"
                     /translation="MKVTTDKIVNEKLSAAIFIQFTVLTIQHLVLKYFGLEGSGAETY
                     IKLFSKLIVGIFFIRTIFDIYYREYKLIIFIYIFSATIFLINVLLFPQNINELKSILF
                     DFFIMCLPCFLFSFSIDNYDHLLNTLKKHGRLIFIVGIFMSYLIIFKNLNIGSNNYSM
                     SLSYYLLIPSLIYLYYFLKDYTFLNFLLFVFSTIGILLIGARGPILCIVVYIIVFIFN
                     NIRGYNSKGRLMFYLASIFIFCIGIIFFNDIIYLVSKVLDYFNIYSRTINFLLNDTFN
                     LSEREKLYFGAFQLIREHPLIGIGIAGDRLYFQGYVHNLFLELILNYGIILGFSFSIM
                     LIYIFAKSIISVDKETSNFNLIFICLGVIPLMFSSSYLVNVWFWIYLGLILKTIFKFN
                     KRNYSL"
     gene            complement(12591..13649)
                     /locus_tag="PKH42_03835"
     CDS             complement(12591..13649)
                     /locus_tag="PKH42_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA19595.1"
                     /translation="MKVLLVDTAVSGHHVSYLKALISNENCDFVICIPKKISDIETKQ
                     IEIKNANFNSGNFIQYYKWIKEIQSAANAEKPDIIQFLYGDIFYRYFGIGLSKFKKYK
                     TVVTFHQIRNSFLRNISIKTISKKINTGIVHTEELLRKLNSLDINNAKHIEYPHLNDI
                     NYITKEFALKYLGLSDKTPVLLALGGTRFDKGLDILLEALKYADKPFQLIIAGQEQYF
                     DKSFIENKIETYKDNVKLLLKFLSDEEFSICINACDFIVLPYRKIFTGASGPLGEGVW
                     LRKTIIGPNHGSLGDIITKNNIGITFTSEDSYMLSKVINDQLNKKYEWNDIAENYRLT
                     LSVYKFKNDYQVIYDELI"
     gene            complement(13678..15003)
                     /locus_tag="PKH42_03840"
     CDS             complement(13678..15003)
                     /locus_tag="PKH42_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOA19596.1"
                     /translation="MNIILLSGGSGKRLWPLSNETRSKQFLKLLKDNEGKPESMVQRV
                     YRQIKEASIESNIVVATGKSQLDSIRSQLGNNVDVVLEPERRDTFPAIVLSCAYLYYK
                     NGMNNDDVVLVLPVDPYANLDYFKALIKMERAIIDKNSNIALMGIKPTYPSAKYGYII
                     PSKYGNLVERFQEKPSEEYAEKLIEEGAMWNGGVFAFKSGYLMNIAKKYIDFNSYEEI
                     EKKYVDLPKISFDYEVVEKEKSIAMVEYQGVWKDLGTWNTLTEVMDCNTLGKVILATN
                     CSNTHVINELDIPVTVLGANNMVIAASPDGILISDKHESSFLKPYVEKLKQRPMYEER
                     RWGEYIVLDYVAYDDGTSSLTKSLFIKAGKSISYQSHKIRDEIWTIVDGMGDLLIDGH
                     IRNVRRGDVAYITKGQKHAIRATSNLRLIEVQIGVELVETDIERHAWKW"
     gene            complement(15023..15967)
                     /locus_tag="PKH42_03845"
     CDS             complement(15023..15967)
                     /locus_tag="PKH42_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOA19597.1"
                     /translation="MLNKDSKIYVAGHRGMVGSAIVRKLNSEGYNNIIIRTSKELNLK
                     RQSDVENFFAEENPEYVFLAAAKVGGIVANNTYPADFLYDNIIIEMNVIKSAFDNKVK
                     KLVFLGSSCIYPRMAPQPIREDSLLTGALEPTNEAYALAKISGLKFCEYLNRQYGTDY
                     ISVMPTNLYGPNDNYHSENSHVIPGLIRRFHEAKISGTKEVIVWGTGKALREFLYVDD
                     LADACVYLMNTYSDNETVNIGTGKEISIAQLAEVIKNVIGYEGTITFDTKKPDGTPRK
                     LLDLSKLNKLGWNYKTEIEEGLKKSYDDFLSNEVRTER"
     gene            complement(16005..17021)
                     /gene="gmd"
                     /locus_tag="PKH42_03850"
     CDS             complement(16005..17021)
                     /gene="gmd"
                     /locus_tag="PKH42_03850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOA19598.1"
                     /translation="MNKKALITGVTGQDGSYLAELLLEKGYYVHGLERRSSIDGQGRI
                     RHLLNNKNFHMHNGDLSDSLSIMRVISKVKPDEIYNLAAQSHVQVSFEAPEYTADVDA
                     IGTLRILEVVHQLNMDKTCRFYQASTSELFGKVQEIPQKETTPFYPYSPYACAKQYAY
                     WIVKNYREAYGMFAVNGILFNHESERRGETFVTRKITLAASRISKGLQDKLYLGNLNA
                     LRDWGYAKDYVECMWLMLQHDTPEDFVIATGEQNSVRDFCNLAFKFAGIDLIWEGSDI
                     NEKGIDKKTGNIIIEVDKRYYRPTDVATLLGDPTKAKSLLGWNPRKTSFEELVKLMME
                     HDLD"
     gene            complement(17053..17820)
                     /locus_tag="PKH42_03855"
     CDS             complement(17053..17820)
                     /locus_tag="PKH42_03855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008245873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOA19599.1"
                     /translation="METARPLVSLITVSYNSEKTIADTIESILEQTYDNIEYIIVDGL
                     SSDNTVSIAKNYETEFKDKGYSFIIISEKDSGLYDAMNKGIRLSTGDIVGILNSDDFY
                     VNEYIIEKVVKMMMEENSDCLYADLLYVDEVNLEKVVRRWKANKGDFRLGWNPPHPTT
                     FITKRSYDKYGLYKTDYKISSDYDILYRIIHKGKVKTSYLEEYIVKMRIGGKSTSGIK
                     SNIISNKEIYNTLKENNQKFKLPIIITRLLVKIKQFL"
     gene            complement(17852..19267)
                     /locus_tag="PKH42_03860"
     CDS             complement(17852..19267)
                     /locus_tag="PKH42_03860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOA19600.1"
                     /translation="MKFNIKNSSFIFKFIEELLYVVFVVLGYYLAFLLRYNMNPLLFN
                     LQPFYDNIPFIVLTSIAIFYIYNIVSTVKKSVFENALTIAIALFLIDIIVVAIVFFNR
                     GFSFPRSVILLGYIIQFILIFTVKIIIIKLIKSNRKQNNILIIASKEESEELAVKLLL
                     DKVNVDNLKYISNNVNDETYDLIDNSEKIYIGNNININNKLNVIKYCSENQKDIYLIP
                     GLYELAMMNSKVNQIKDILFLKVDKLGLTFEQILVKRIIDVAASLIGLILLSPLLIIV
                     AITVKVYDRGPVFYKQERVTIGNKSFKLYKFRTMIIDAEKKTGPVLATDNDDRITPIG
                     KFLRATRVDEFPQLINVLKGEMSLVGPRPERPFFVEKHNKEIEEFKYRVYVKAGITGL
                     AQIMSNYSTNAKNKAKYDLLYIRNYSLMLDIIIIFNTVKIMFMKDSSVGVTKYKNLED
                     ILNEQDLKAKEEMGITKVDNI"
     gene            complement(19281..21131)
                     /locus_tag="PKH42_03865"
     CDS             complement(19281..21131)
                     /locus_tag="PKH42_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA19601.1"
                     /translation="MKKKNIKTKQKQQPVAIDYENKKLSAAYFLPLLLIAGFVPLIVY
                     AKHIDLEGTTQALYWTGQRQYLDFFSYWKSRWTVVLTAIALILYIILYKQKKLPFKNL
                     KQYYIPLGIYAICVIISTILAIDTQTALWGFVDMYQGMFVLISYVLITFLTINFVNSE
                     RDVTLFVKAFLVLMIIEGLIGITQYFGFDFFQTKIGTGLIVPGRLHVENLSFPFGPKT
                     IYGTLFNTNFVGSFATLMLPLSIAFLLGAKTKKQRIVSAIAVVLMIFVWIGCNSRAGY
                     LGVAVASIFAVWLFRKLIIKFWKASASIAAILLIIVIAMNTVSQGALFDRIKTFNIVE
                     QIKSVKAKDQTAFKFEDIELGKDTISIKTNIQTLNMRLDNNKLYFLDENMIELPIKTV
                     GNKIEIQDVNKYWQLNNIKIPENYPGISITTHWGWGTINFLFSQDGVKIIGSGGRLTE
                     PIVAESFKPITGYEKLMSDRGYIWSRTIPLIKNNFITGSGADTYPIAFPQDDFIAKLN
                     IYMDASMVVDKPHNMYLQTAINTGAISLMAILVIIVMYIVTGLKLYNKTNFKVYEEYV
                     GASCLISVIGYLTAAFFNDSVVSVAPLFWLILGLGIGINYKLIKKNHTLE"
     gene            complement(21386..>21634)
                     /locus_tag="PKH42_03870"
     CDS             complement(21386..>21634)
                     /locus_tag="PKH42_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19602.1"
                     /translation="DSTKAGGEKFSWVVEALYELIPSPLKIIVTKKCIEKIVQNSFDA
                     IENYAKMQLDIAVEKYLKYLNDGKSGDFIDFIRDSDVE"
BASE COUNT         6797 a         3843 c         2903 g         8091 t
ORIGIN      
        1 ctatcgaatt attgataggt tttacgggag gaattgccac cacctatgtt catcagcatt
       61 taaaggaagg agatacagct catattaacg gtccttatgg tgatttttat tatcatgatg
      121 atgatggccc gattatattg gcaggtgcag gaaccggaat ggcgcctata atgtcaatcc
      181 ttcaatatat ggctgataac aatattatga gggaaactat atttttcttt ggtgccagga
      241 caatggatga tctattttta gttgatagga taaaaggttt tgaagaaaaa cttaatctaa
      301 agtttgtacc tacactatct cgggaggaat cccctgagtg gaagggagaa aaggggcgtg
      361 tacctaatgc cattgataaa tatgttgaaa atgcagaaaa ttactcagca tatctatgcg
      421 gcagccctgc aatgattgat tccatagttg aggcattggt gaaaaagaat gtacctgtgg
      481 agaaaatata tttcgacaag ttttaaggta tttaagattg ttcttcattc tttatcgcca
      541 ttttcaaatc tgcagcacca caagttgtac gcatataatt actacaccag ttattttctg
      601 cctgagcttt taataatttc acatatatat tttcaaaaat ggagtttaca tgtattcgct
      661 gataagtgtt taatcaaagt cccatattat agccattgta tttcgttcca atttaaacct
      721 ctttaactac cacttcccaa taacccaatg tatctgaacc cactcggaca ataatatcgg
      781 cttcttgaag ttttttcata tttctttcaa ctgttctaat agttttattt aactttttac
      841 ttatctctgc ctgggtaata tgcttatcag acttaatgat gctaataatg cttttttgtg
      901 tattattgat cttaatcatt gtctcaccga cattttcacc gacattctca ccgacattct
      961 caccgacatt ctcaccgaca ttttttatag ggaaactaac ttttaaaaaa tttggactaa
     1021 acttaaatac atttttatta taaactttta atattctctg aacaccagaa ccaagttgct
     1081 ctactaattt aacatctttg aacaccctca taagttcctt atttctaggt gcagaaattc
     1141 catcaaaaaa ttcttcttga cttaattctt gcggcaaccc tcctgatgac gtaataacaa
     1201 atttatcaga gaatatttca aaaactggag gtattccgtt agaataatca ttatgtatta
     1261 ttgcatttat tacagcttcc cttaatgctg tagaattaat aagacgcttt tcttcataaa
     1321 ctacttactt tagcagtaat accataacac tcgaactttg tcacaaaatg cttattttgc
     1381 ctttaataac tcaagtttaa ttaaccaacg aacaaaatca atcgctatga tcattgaaac
     1441 ttcaatcaaa aataactcaa gtcaatggtg cctggcacgt caatggtgcc tggcacggcc
     1501 aatgtgaatg tccatccaag agcatttcta caaaatttat ggccgcgttc ctaatgccat
     1561 tgataaatat attgaaaacg gaaaaaatga agcattgtta aaaaagaatg tacctgtaga
     1621 gaaaatacat tttgataagt ttaaatcaat ttgtttatat tttttctata atacttcttg
     1681 ggtttttttt gagttttagc tttcatattt ttgactattg ggttacttgt attccgatca
     1741 gttgtttttt gaggaaccca cattcttctt agtccctaca atacaaatcc ctggtataaa
     1801 ccttatcttc aatatctctg atgtcattag acattctatt ggcaacaatg acatcggata
     1861 ttcttttaaa ctcatctaaa tctctgatta ctcttgaatt gtagaagctg tcatctttta
     1921 ataccggctc gtatacaact acttcaatac ctttaccttt aatacgtttc attactcctt
     1981 gaattgagga ttgtctgaag ttatctgaat cagttttcat tgtaagtcta taaataccaa
     2041 ctatcttagg atttcttttg ataatcatat cagcaatatg gtcttttctt gtacggtttg
     2101 catccaccac cgctgtcatt atattctgcg gcacatctgt ataattagct aaaagctgct
     2161 tggtatcctt cggcaagcaa tatcctccat atccaaatga aggattgttg taatgacttc
     2221 ctattcgcgg gtctgaacat accccttcaa taatctgctg tgtgtttaat cctctaacct
     2281 ctgagtaagt atctaattca ttaaaaaatg atactcttaa tgcaaggtaa gtattagcaa
     2341 ataacttgat ggcttccgct tctgtagcat cagtataaag gattgggata tctttcttaa
     2401 tggcaccttc agctaatagg tttgcaaaaa tctctgctct ttcagatttc tctcctatca
     2461 caattcttga aggataaaga ttatcatata aagcctttcc ttctcttaaa aattcaggtg
     2521 aaaaaatgat attatcagta tcaaatattt ctttaatctt ttctgtatat ccgaccggca
     2581 cagttgattt aattaccatc actgcttcgg gattaattct taatacattt gtaataacct
     2641 cttcaataga gctggtatca aaatagtttt tattttcatc ataatttgtt ggagttgcaa
     2701 taattacata ttctgcattt ttatatgctg tgttactatc agtggttgct cttaaattta
     2761 aactcctgtt tgtcaaataa tcctgtattt cagcatcaac tatgggtgac tttttattat
     2821 taatcatatc agctttttct tgagttctat tcaatgcaat tacctcattg tgctgagcta
     2881 aaagaactgc gacagataac cctacataac ctgttcctgc tactgctatt ctcattttgt
     2941 gttctccttc tgctttttag tcattatctc taattttata ataatccata taccaattta
     3001 caaacttcct aagtccatct tcaatacttg ttgatggttt aaaattaatg tctttttcaa
     3061 ggtcagacac atcagcatat gtccttagaa catctcccgg ctgcatatcc atataaactt
     3121 tctcagcttc tttccctaat gctgattcta aagcattgat aaatctcatg agcttcaccg
     3181 gattgttatt tcctaaatta taaatcttat aaggtgcaaa gcttgtactt aaatcatctt
     3241 tgctttcatc ccattcttta ttcggaacgg gagctttacc tataagcttc acaatacctt
     3301 caactatgtc atcaatatat gtaaagtctc tttccatttt tccatgattg aaaactttaa
     3361 tcggcttccc tgctaatata tctttcgtaa atgaaaaata agccatgtcc ggtcttcccc
     3421 acggtccgta aacagtaaaa aatctaagtc cggttgtcgg aattccataa agatgactat
     3481 atgtatgggc catcaactca tttgatttct tagttgcagc ataaagtgat acaggatgat
     3541 ccacattatg attagttgag aatggaacaa cgttattgcc gccataaaca gagcttgaag
     3601 atgcataaag caaatgatcg accggataat ttctacaagc ctctaagata ttgatgaagc
     3661 ctaataagtt agagtcaaca taagcataag gattctcgat agagtaccgc actcctgctt
     3721 gagccgccac atttataacg tgagtgggac gataagtttc aaagataata tcaattgatg
     3781 ctttgtcttt caagtctact ttttgaaaat taaaattgtt gtactcccct aagatttcaa
     3841 gtcttgaatt ctttaggctt ggatcataat agtcatttag attatcaatc cctacaactt
     3901 cataggattg atcaagtaat ttccttgtaa ggtgaaatcc gataaagccg gctgcacctg
     3961 ttattaatag tttcataaga tttctccaat tataatatct attattaatt tttcatatca
     4021 cagtttatag aataatcaag aaattgctct gctttttaca aactatattg actggtgact
     4081 ttgttaaatt caggaaatat ttattttaag acattacagt acattttaaa tcattttcgt
     4141 attagaatca aggtgaaaat aagttttttc catattttta ctaattctac ataatagata
     4201 tttaaaaggc atgcttagtc cctgctaaac aagtttctgg tatataccta ttgggttggt
     4261 tgtggtgatt tatctattgg tatggttttc catattaaag atttctaata aggaataaat
     4321 atttgtatgt gttgtataga ttatatttca ataaatatga tataagctga tgtcttttct
     4381 tgccggattt tgaaatttca ttaattaatt tatttttgag ctgcttgtct tctaacaaca
     4441 cactcagagg gatgccttgc tctattgcag aatttaaact acttacatat gcatatctta
     4501 aatgtttttc aaagaaatca taaacatctg cctgggtttt tttgtcgata ttgtcattgg
     4561 caaagtcttt gtacctgtcg cctattttga ctaaatcagt gcactttttt atatttttgg
     4621 tatgcataaa agacccttgt ctttgaacat aattatagaa gcataaatca attgctttta
     4681 tcctttttgc aaaatataat gccctcggga taaattcaaa atcttcatga tatatatctt
     4741 cactaaacca taaattgtta tcaatcaggt actctctgcg gaacaaataa agccacacta
     4801 caaaattaaa attctttctt accagccatt gaaaaccgct taaacaatca cctatgtttc
     4861 ctcgtccgtc gttacatttt cttataacaa tacttccttt atcatctact aacctatagc
     4921 tgccttgaac tatatctaaa ttttcttgtt cagctgcatt gtataaagct tcaagggagt
     4981 tttcattaat ataatcatct ccgtcaataa aaataacata tttgcctgtt gcattcttca
     5041 aaccataatt tcgggcagaa gcacagccgc cattatcctt ttgcagcagt tttattcttt
     5101 gatctaaatg catatatttt ttgattattt ctacgctttt atccttgctt ccatcatcca
     5161 catagatgat ttccatatcg atattctgtt ttattattga ttcgatattt tttgtcaaat
     5221 acttctcgat cttgtaaact ggaactatta cacttacttt attgtcttca cgatttaaca
     5281 ttcagccatt ctccataata tttgatcgtt ttggttaatc catcatttaa tgatactttc
     5341 ggttcccact tcaaagattc tctagctaaa ctgatatctg gttttcgtct catcggatca
     5401 tccttgggta atttttgaaa tactatttta gattttgaac ttgttaatct tataattgct
     5461 tccgcaagtt ctaagatgtt gtgttcttca ggatttccta tgtttacagg acctgtaaag
     5521 ttgctttctg tatccatcat ttttatcaaa gcttcaatta aatcatcaac aaaacaaaag
     5581 cttctggtct gactgccgtc accgtaaatt gttatatctt catttttaag tgcttgaata
     5641 atgaagtttg acactactct tccgtcattg aagctcattt taggaccgta agtattaaat
     5701 attcttgcaa ttttcacaga aacattgtac ttgcgataat aatccataaa aaatgtttca
     5761 gcagctcttt taccctcatc ataacatgat ctcaatccca acggattaac atttccccaa
     5821 taatcctctt tttgaggatg cacttgaggg tctccgtaaa cttcactggt actagcttgc
     5881 aatattttag aattagtgcg ttttgccagt cccaacatat taaccgatcc aaatatgctt
     5941 gttttggcag ttttgatggg gtcgtgactg taatgaaccg gacttgcagg acatgctaaa
     6001 ttatagattt catcacattc aacatatagt ggagttacta tgtcatgccg gatgaattca
     6061 aaataattat tattaaacaa atgtctgata ttttctttgc tgccggtaaa aaggttatca
     6121 acacagataa catcatttct ctcgatcaat aatctttcgc acagatgcga ccctaaaaat
     6181 cctgctccgc ctgttacaac tattctctta gccataatta tatttgatgt gtcggtttaa
     6241 cacatcattg ccccctcttg tttattagat taaaaacttt tttgttgaaa tatttcacta
     6301 agtcggtaaa gtaattcatt tcttccacct tcatcaacag catcaaaaca aaatatattg
     6361 atgctcctgc tgctgcagat gctgcaattg ataaaagagt tataattttg cctggttcaa
     6421 atgatgaatt taaataatta aatgtaaaat aaactaaaag ccccataaca actgaactga
     6481 tacaggattt aatcaatata gtcaaactgt cttttaatcc taaattgccc aaacgtattc
     6541 ttaaaaaata aaaaaacaag ggcaatgtta tgatgtctga tagactggca gcaagtgcaa
     6601 gaccggcatg ttctaaaggt ttgattaaaa ttgtactgaa aattatgcat gcaaagacag
     6661 tcaaaaaact gatatacatt atggctttgg tttctttcat ggagtagaat acttttgtta
     6721 taacatctcg caaggcaaca gctgccaacc ctatggaata catctgcagc gcagcagatg
     6781 tcataagggt tgattggctg ctgaattctc ctctttgata aacaaaaaat attgcaggct
     6841 cagacaatat aattgcaccc acagatatgg gaataaacag tattattatg atggtgatag
     6901 ctttggaaac taatcttttg atggtattta tatcgttttt tgccatactg cttgaaagca
     6961 tgggataaat catagtggtt aaagcaacgg taaatatata aaaaataaag taccttatga
     7021 cacccgaata attcaaggct gatatgctgc cttcagctaa atttgatgca aatgctttat
     7081 tgacaaaact atttatctga attgtcatgc tgctgataaa tattggaacc aatatactga
     7141 aagcttgctt gatttcaggt gttatcttca gttcatactt gtaaacatat ccgtttttat
     7201 aagaaaaaaa taataacagc gacaactgtg ctatatatga caatattgtg ccgtaaatca
     7261 aaatttcttt gcccaatttg ctacttatat agactaccaa tagctgagtg ctgctgatta
     7321 tcagagtaga tatattggat gcaataaatt tattgtgaca gtttaaatag gatataagta
     7381 tttgtacaaa aacattgatt gatatggaaa aaacagttac ttttaagaat ctgtttgtaa
     7441 ggttgtaaac ttctccttca aatcctggtg cagccaagct tactatctga ttgttgaata
     7501 gaacacctat aaaggcacat attatggcaa ttgtaatggc tatggatata atattgttgt
     7561 taaacctctt ggcattgtct tctccactct ttactcttac ttccgtatag attggggtat
     7621 atgatacaga taatgaaaca atccacccaa aaacaattcc gggtatggct atagccatca
     7681 aatatgcatc aacaacatat gatgtgccat aaaagtaagc taaaacaatt tctctggcaa
     7741 atcctaacaa ttttgaaccc aagcttatca ttgttattat tatggctgtt gtttttatgg
     7801 ttgacattat ttcctcgcgt tgatctcatg ttgcaaaact accgcaacac aaacctgtta
     7861 ttctcattgt tgttctttaa attctatggc tctgcatgaa atatccttgt atgaaaaaaa
     7921 tttcattccc tccgattcgg ctatttccct catatgatcc agctcgtctt tgttgtttat
     7981 caaactcatc aataatttat aaatttcatc gacactgttt ccatataaaa atttacagtt
     8041 gccacctaaa tccacatgat ttgttccttc ccagtgtcta acaatcaagg gaattcccaa
     8101 tcctgctgct tgttcccata ataccgaatg cctgccggga aagacaacta aatctgatgc
     8161 agcgaaatac ttataacttt ctgatgattt tatccatcct acatactgta ctttaactcc
     8221 atctgctaac ttattaattt catctttcaa ttcttttatt acagaaccaa aaactataag
     8281 ttttaaatta tcatagtcta ttttctgaac agcttccatt aaatccagaa tctgtctttt
     8341 ttctttgtca attttaccgc cggtaataat caataaatct tcgtttttta tattgtattt
     8401 gtttcttact tcatgtttga tgtccgggtc tttagcttct gttacttttt catcatctgc
     8461 ccccatgaca agcagttcaa ttttttcttt cggaagttta taaacgtctc ttaagaaatc
     8521 cactctggta ggaataaccc catagaattt tttagtataa ggctctattt gatgagcaca
     8581 atatttccag attattttat gaagaatatt tttggacaaa aaatttcttg cactgttgga
     8641 aaaatcggcg tggttgtcta catatattcg tgtgttcgga tgaagtttca catatttgac
     8701 aacatgcttt atatcaagga actgacaacc gtgggcaaat ataatatccg gttgtgtttt
     8761 gcatattgtt tcatatgtat tggtgtaggt tcttagagtt ttgctgtatt ttttctttga
     8821 ataatttagc ctcgtgacag gtatgccata ttcatttata tagctgcctg attcattcaa
     8881 caaattagga tttccgtcct tgtcaaaaga aaccaatgat gcgattattt caacatccaa
     8941 gcccatatcc ttatgaaact taggcaacat gttttcttgg taagaataat tatcaatata
     9001 aaaacttgct aaacataaat gtagtatttt caaagttgta ctccttaatt acttaatgtg
     9061 gaattttaaa tttttcaatg ttattgaaat ttaaagcagc agcttgaaga gctgcatcat
     9121 ccatattcca ccaattgctt tttattaaaa tatctattgt ttcatcatca aatcttttgc
     9181 gaattaattt agcagggtta cctgcccata tttcataatc gcccacatct tttgtaacta
     9241 cagagcccat tccaataatg gcaccgttac ctattttgat tccaccttta attaaacaat
     9301 tattacctat ccatacatca ttgccaatta ttgttttttg tcctgtttca aattcatgtt
     9361 cagaaaaatt ctttctcatt atatttttac cttcatgaaa tactggtgaa gttgataccc
     9421 aatctatggg gtgacgatgt ccgccaataa tgcaattatc tgcaattgag caaaaattac
     9481 ctatgtcaca gttaaaaacc gagcaaaaat ttgttatata tgtgtatctt cctaatgtag
     9541 aattaacaat atgagaacct gagcatacct tagctgtttt atctattgta gaatttttga
     9601 ttgcaggtat atgaagtttt tttattagct tagaaatcaa aaagcttaga ttcataaatc
     9661 caccacctta aactatttca attttatttt ttatttacat ctaaccaata ctccttaatt
     9721 agagttatta aaaccccaat aaacaaacct atcgacaatc ctattgctgt atttaatgca
     9781 atgcttggac tgctcttaaa tgttggagca acaggaggtg aaggcaaata tatattagct
     9841 gttactgaac ttactatatt tgattccaat ttttttactt taccggtttc ataatattct
     9901 tttacagact gtaattcttc ttctaattcc gttagaaaca tattattcat cctaatgtga
     9961 ttttctattt catttattga ttgcttattt tgtataatat cactctcaat acttatataa
    10021 ttaggattta ttgcatttat aggtataaca taatcaattg attcatttaa acttcttttt
    10081 atatctatac tgctgccttc ttgtgataat gatttcggaa tttcattcaa tagttcttcg
    10141 ttccttttta aaatttcttt attactattt aactgttttt ctaaattttt attttgcaga
    10201 gtaaattcat tgtagtaatt attaatagct acttctttta tcattacgtc aataaaatcc
    10261 aaataacttt tatatagatt ttgggccaat tttaacgact cttcaggggt tctttcagat
    10321 actataattt cgaagctgtt ttgttttact ccagaagcag cttttgtctc taaaattgtt
    10381 actttccctc tcaaatcttc tatactgaca tcattatcat aatccatgta ttttattgtg
    10441 ttagcaataa catcattgct ttttattaaa ttaagatatt gactattatc aaaaaaaggc
    10501 aactcatatt caccatatct tgttgtataa gtttccggca tgctaattat tatatttagc
    10561 tttgtttcat atgtgggaat aatcacaaac ttgctatata aggctacaat taatccaact
    10621 atcaatgata aacttataat catttttttc cttttccata aaactaaaaa tatttctttt
    10681 aaatcagttt gatatacata atactgaact tctttttcat ttttgttcat cttatttact
    10741 cctttagtta ttaaatcgta attattataa agaataattt cttttattaa atttaaatat
    10801 tgttttcaaa attaagccta aatatatcca aaaccaaaca tttactaaat aagagctgct
    10861 aaacatcaaa ggtatcacac ctaaacagat aaatattaag ttaaaattag atgtttcttt
    10921 atcaacagat ataatagatt ttgcaaatat atatattaac ataatagaaa atgaaaaacc
    10981 taaaattata ccataattca atattaactc taaaaataaa ttgtgaacat aaccttgaaa
    11041 atataaccta tcgccggcta ttcctatgcc tatcaaagga tgttctctga ttagttgaaa
    11101 agctccgaag tatagctttt ctctttcact taaattaaat gtgtcattta gcaaaaaatt
    11161 aatagttcta ctgtatatgt taaaataatc cagaacctta cttactaagt atattatatc
    11221 attaaaaaat ataattccta tacaaaagat aaatatagat gctaaataaa acatcaatcg
    11281 tcccttgcta ttatatcctc taatattatt aaagataaat acaataatat aaacaacaat
    11341 acataaaatc ggacctcttg ctcctattaa taatattcca atcgttgaaa aaacgaatag
    11401 taaaaagttt aagaaggtat aatcttttaa aaagtagtaa agataaatca atgatggtat
    11461 taataaatag tatgataatg acatgctata attgttgctg cctatattaa gattcttaaa
    11521 tattatcaaa tatgacataa atatacccac tataaaaatt aatcttccat gttttttcag
    11581 ggtatttaat agatgatcat aattatcaat gctaaagcta aataaaaaac atggtaaaca
    11641 cataataaaa aagtcaaaca gtattgattt taattcatta atattttgag gaaacaataa
    11701 tacatttatc aaaaaaattg ttgcggaaaa aatataaata aaaattatca gtttgtattc
    11761 tctgtagtaa atatcaaaaa ttgttctaat aaaaaatatc ccaacaatta atttcgaaaa
    11821 tagcttaata taggtttctg caccacttcc ctctaaacca aaatatttta aaactaaatg
    11881 ttggattgtg agtactgtaa attgtataaa aattgctgca gataactttt cattaactat
    11941 tttatccgtt gttactttca ttaaatactc caaattcgtt aagtttatta tttctttaat
    12001 tattataaca agttatgagt cgttttaaaa tataaaagca cttaaattct atctatttaa
    12061 taaaattatt taccaaacct aataattttt cctttttaat atcatatgta aacagacttt
    12121 gtgctcgttg ccttgcttta atagacaatt tatctcttaa attatttaaa atttttttag
    12181 gaagttatgt ggtgctattc tttttaaaat atttataatt tcttgtttca tttcttgatt
    12241 taacgttagt ataccaaagt aaaatatcga gcataataat attgagaata aatcccaaac
    12301 tatccaattg cttatttgtt gaagaactga tccaaataaa aacattaata gtgttgaagc
    12361 agctgttaaa gatacattct tgatcatttt aaatatagga aatttaataa taaacttcaa
    12421 tataattaag tgaaccaata acatctctaa tcttatccat gatctaacat aaactaatgg
    12481 ccaaaaacca taattagaac ttattataca agcaggaatt aaaacaacta aatgaagttt
    12541 tcccacagat attactcctt atataactta ttcttaaatg gttatttata ttatatcaat
    12601 tcatcgtata ttacttgata gtcattctta aatttataaa cagacaatgt aagtctataa
    12661 ttttccgcaa tatcattcca ttcatatttc ttatttaact gatcattaat tactttagat
    12721 aacatataag aatcctcaga agtaaatgtt atgcctatat tatttttagt aataatatca
    12781 cctaaacttc catgattagg tccaattatt gtttttctta accatacacc ttctcccaaa
    12841 ggaccgcttg ctcccgtgaa tatttttctg tagggcagta ctataaaatc gcaagcattg
    12901 atacatattg aaaattcttc atctgataga aattttaata atagttttac attatctttg
    12961 tatgtttcaa ttttattctc tataaatgat ttgtcaaaat actgctcttg acctgctatt
    13021 attaattgaa acggcttatc cgcatatttt aaggcttcaa gcagtatgtc aagacctttg
    13081 tcaaatcttg ttccccccaa tgccaacaaa acaggagttt tgtctgacag acctaaatac
    13141 ttaagagcaa attccttggt tatataattt atatcattta agtgaggata ctctatatgc
    13201 ttggcattgt taatgtctaa actgtttaat tttctcaaca attcttctgt gtggacaata
    13261 cctgtattga tttttttgga tattgttttt atgctgatgt ttcgtaaaaa actgtttctg
    13321 atttgatgaa aagttactac agtcttatat tttttgaatt ttgataaacc tataccaaaa
    13381 tatctgtaga atatatcgcc gtataaaaat tgtattatgt ccggcttttc tgcattcgct
    13441 gcggattgga tttcttttat ccatttataa tactgaatga aattgcccga attaaaattt
    13501 gcatttttga tttctatctg ttttgtttct atgtcactga tttttttcgg tatgcaaatg
    13561 acaaagtcac aattttcatt gcttataagt gccttcaaat aagaaacatg gtgaccacta
    13621 actgccgtat ctactaataa aaccttcata tattctcctg aaatattact ccattaatta
    13681 ccatttccaa gcatgacgtt caatatctgt ttcaactaat tcaacaccaa tctggacttc
    13741 tatcaaacgc aaattagaag tagctcttat tgcatgtttt tgtcctttag taatgtacgc
    13801 tacatccccg cgcctaacgt ttctaatgtg tccatcgatt aaaaggtctc ccatgccatc
    13861 tactattgtc caaatttcat ctcttatttt atgtgattga tagcttattg actttcctgc
    13921 tttaataaac aaactttttg ttaaggatga tgtcccatca tcataagcaa cataatcaag
    13981 cactatgtat tctccccatc gccgctcttc atacattggt cgttgcttaa gcttttctac
    14041 ataaggctta aggaaagaac tttcatgttt atctgaaatc aaaataccat ccgggcttgc
    14101 agctataacc atattattag cccccaatac agtgacaggg atatctaatt cattaattac
    14161 atgtgtgttg ctacagtttg ttgcaagtat cactttccct aaagtattgc aatccattac
    14221 ctcggtaagt gtattccaag ttccgagatc cttccataca ccttgatatt caaccattgc
    14281 aattgatttc tctttttcta caacttcata gtcaaagctt attttaggca ggtctacata
    14341 tttcttttct atttcttcat atgaattgaa gtcaatatat ttttttgcaa tgttcataag
    14401 gtatcctgat ttaaatgcaa aaacaccgcc attccacatt gcaccttctt ctattaattt
    14461 ttcagcatat tcttctgaag gtttttcttg aaatctttca actaaatttc cgtatttgct
    14521 tggtattata tatccgtatt tggcagatgg gtaagttggc ttaattccca ttaatgcaat
    14581 atttgaattt ttatctatta ttgctctttc catctttatc aatgctttga aataatccaa
    14641 attggcatat gggtctaccg gcaatacaag aactacatca tcgttattca taccattttt
    14701 ataataaagg taagcacatg ataaaactat ggcagggaag gtatctcttc tttcgggctc
    14761 aagcacaaca tccacattgt ttcctagttg gcttcttatg gaatctaact gtgacttccc
    14821 tgtagcgaca acaatatttg attctatgga cgcttcttta atctgcctgt atactctctg
    14881 aaccatggat tcgggttttc cttcattgtc ctttaacaat ttaagaaatt gctttgatct
    14941 ggtttcgttg gaaagtggcc acaatctttt ccctgaaccg cctgataata gaattatatt
    15001 catttgttta tctcctgtct aattatcttt ctgttcgaac ttcattactt aaaaaatcgt
    15061 catatgattt cttaagacct tcttctattt ctgttttgta attccagcct aatttattta
    15121 gttttgaaag atctaatagt tttcttggcg ttccatcagg tttttttgtg tcaaaagtaa
    15181 ttgttccttc ataccctata acatttttaa taacctccgc aagttgtgca attgatattt
    15241 cttttcctgt tcctatattt actgtttcat tgtcagaata tgtattcatt aagtaaacac
    15301 aagcatctgc taaatcgtca acataaagaa attctctcaa tgcttttcct gtgccccata
    15361 caattacttc ttttgttcct gaaatttttg cttcatgaaa acgtctaatc aaaccgggaa
    15421 tcacgtgaga attctcggaa tgataattat catttggtcc gtatagatta gttggcataa
    15481 cacttatata atccgttcca tattgacgat ttaagtattc acaaaatttt aacccagata
    15541 ttttagctaa agcatatgct tcgtttgttg gttccagggc acccgtaagc aagctgtctt
    15601 ctcgaattgg ttgtggtgcc atgcgaggat aaatacaaga agagcctaaa aaaactaatt
    15661 tctttacttt attatcaaat gcacttttaa taacattcat ttcaattata atattatcat
    15721 acaaaaagtc agctggatat gtattgtttg ctactattcc gccaacctta gctgctgcta
    15781 aaaaaacata ttcaggattt tcttcagcaa aaaagttttc tacatctgat tgtcttttta
    15841 aattaagttc tttagacgtt cgtataatga tattattgta accttcacta tttaatttcc
    15901 ttacaattgc agaaccaacc attcctctat gtcctgccac atatatttta gaatctttgt
    15961 ttaaaatttt ttttgatatc attttaattt tgcctctcgt tcagctaatc taagtcatgc
    16021 tccatcatta attttaccag ttcttcaaat gacgtttttc gtggattcca tcctaacagt
    16081 gatttagctt ttgttgggtc accaagtaat gttgcgacat ctgttggacg atagtatctt
    16141 ttgtcaactt caattataat atttccggtt tttttatcta tgcccttttc gtttatatct
    16201 gaaccttccc atatcaaatc aattccagca aatttaaagg ctaaattgca aaagtcacgt
    16261 actgaatttt gctcgcctgt tgcaataacg aaatcttcag gggtatcatg ctgcagcatt
    16321 aaccacatgc actcaacata atctttagca taaccccagt ctctcagggc atttaaattt
    16381 ccaagataca atttatcctg cagtcctttt gaaattcttg atgctgctaa agtaattttt
    16441 cttgttacaa atgtttcgcc tcttctttca gattcatgat taaataagat cccattcact
    16501 gcaaacatac catatgcttc tctataattt tttacaatcc aataagcgta ttgttttgca
    16561 catgcatatg gagaatatgg ataaaaagga gttgtttcct tttgaggtat ctcctgcacc
    16621 ttcccgaata gttctgatgt tgatgcctgg taaaaacgac atgttttatc catgtttagc
    16681 tgatgtacta cttctaaaat tcttaatgtg cctattgcat ctacatctgc agtatattca
    16741 ggtgcttcaa atgacacctg aacatggctc tgtgctgcca aattgtatat ttcatccggt
    16801 ttgacttttg atataactct cattatgctt aaagaatctg ataagtctcc attatgcata
    16861 tggaaattct tattatttaa taaatgtcta attcttcctt gtccgtctat tgatgaacgc
    16921 cgttctaatc catgaacata gtatcctttt tctaataaga gttccgctaa atacgaaccg
    16981 tcttgaccag ttactcccgt aattaatgct tttttattca tacgtttaac tcctttgtta
    17041 tatattttta aatcaaagaa actgttttat cttaactaat aatctggtaa taattatcgg
    17101 taatttgaat ttttgattat tttcttttaa ggtattatat atttctttat ttgaaattat
    17161 gttgctcttt ataccgcttg tagattttcc tccaatcctc attttaacaa tatattcttc
    17221 aagatatgag gtttttactt ttcctttatg aattatccta tacaaaatat catagtctga
    17281 tgaaatttta taatcggttt tatagagtcc gtatttatca tatgatcttt ttgttataaa
    17341 agttgtagga tgcggcggat tccaacctaa cctgaaatct cctttgtttg ctttccacct
    17401 tcttacaacc ttctctaaat ttacttcatc tacatataat aaatcagcat acagacaatc
    17461 tgaattttct tccatcatca tttttacaac tttttcaatt atatattcat taacataaaa
    17521 atcatctgaa ttcaaaatcc caacaatatc tccggtagat aatcttatgc ctttgttcat
    17581 agcatcatac aagccgctat ctttttccga aattattata aaactgtatc ctttgtcttt
    17641 aaactctgtt tcataatttt ttgctataga tacggtattg tctgacgaga ggccgtctac
    17701 aattatatat tcaatattat catatgtttg ttcaagtatt gattcaattg tatcagcgat
    17761 tgtcttttca ctgttgtatg aaacggtaat taaacttaca agtggtcttg ctgtttccat
    17821 attgttctcc gacaaatata caatctgttt tctatatatt atctactttt gtaattccca
    17881 tttcttcttt agcttttaaa tcttgttcgt ttaaaatatc ttctaaattt ttgtatttag
    17941 tgactcctac tgaactgtct ttcataaaca taattttaac agtgttaaat attataataa
    18001 tatctaacat caaagaataa ttccggatgt agagtagatc gtattttgct ttatttttag
    18061 cattcgttga gtaattgctc attatttgtg ctaatcccgt tattcctgct ttaacatata
    18121 ctctgtattt aaattcttca atttctttat tatgtttttc aacaaaaaat ggtctttccg
    18181 gcctaggacc cactaaactc atttctcctt taagaacatt gattagctga ggaaattcat
    18241 caaccctagt tgctcttagg aactttccta tcggggttat tctatcatca ttatcagtag
    18301 ctaaaacagg tcctgtcttt ttttcagcat ctattatcat agttctaaac ttatataatt
    18361 taaatgattt gtttccaatt gtaacccttt cctgtttata aaaaactggt cccctatcat
    18421 agactttcac tgttattgca actattatca gtaatggtga caataatatc agtccaatca
    18481 atgaagctgc cacatctatt atccttttta ccagtatttg ctcaaaagta agacctaatt
    18541 tgtcaacttt caaaaatagg atatctttta tttgatttac ttttgaattc atcattgcca
    18601 attcatataa acccgggata agataaatat ctttttggtt ttcagaacaa tattttataa
    18661 catttaactt attgttaata tttatattat tgccgatata aattttctcc gagttatcaa
    18721 tcagatcata cgtttcatca ttaacattat tgctgatgta ttttaggtta tcaacattaa
    18781 ctttgtccag caaaagcttg acagctaatt cttctgattc ttctttggat gcaattatta
    18841 agatattatt ctgtttcctg tttgatttta ttaatttgat tattattatt ttgacagtaa
    18901 atattaatat aaattgtatg atgtaaccca gtagtataac gctacgagga aatgagaatc
    18961 ctctgttaaa aaaaacaatg gctacaacta ttatatctat caaaaatagt gcaattgcaa
    19021 tagttaaagc attctcaaaa acagattttt ttactgtaga aactatatta tatatataaa
    19081 aaatagctat agaggttaaa acaataaagg gtatgttgtc ataaaaaggc tgcaggttga
    19141 acagcaatgg attcatatta tatcttaata aaaaggctaa ataatatcca agcacaacaa
    19201 aaactacata taatagttct tctatgaatt taaaaataaa gctgctgttt ttaatattaa
    19261 atttcatatt atcgcctcat ttattccaga gtatgatttt tcttaattag tttataatta
    19321 ataccgattc ctaatccaag tattaaccaa aataatggag ctacgctgac tacactatca
    19381 ttgaaaaatg ctgccgttaa ataacctatt acacttatta aacaagaagc cccgacgtat
    19441 tcttcataca ccttaaaatt tgtcttatta tacaatttta atccagttac aatatacatt
    19501 acaattatca caagaattgc cattaatgat attgctccag tgtttatggc tgtctgcaga
    19561 tacatattat gtggtttatc aacaaccata cttgcatcca tataaatatt aagctttgca
    19621 ataaaatcat cctgtggaaa cgcaatcgga tatgtgtcag caccggaacc ggttataaaa
    19681 ttattcttta tcaatggaat tgtcctactc catatatatc ctctgtcaga cattagtttt
    19741 tcatatccgg ttattggttt aaaactttct gcaacaatag gttcagttaa ccttcctccg
    19801 ctacctataa tttttacacc atcttgtgaa aataagaaat taattgttcc ccatccccaa
    19861 tgtgttgtta tagaaatacc aggatagttt tccggaattt taatgttatt caattgccag
    19921 tatttattga catcttgaat ttcaatttta tttccaactg tttttatagg taattcaatc
    19981 atattttcat caagaaaata tagtttattg ttgtctaatc tcatatttaa agtttgaata
    20041 tttgttttta ttgaaatagt gtctttgcct aattctatat cctcgaattt aaaagcagtt
    20101 tggtccttag cttttacgct tttaatttgc tcaacaatgt taaaagtctt aattctatca
    20161 aacagtgctc cttgagagac agtattcata gctataacaa ttattaataa aatagctgct
    20221 atacttgcgg aagctttcca gaattttatt attaactttc taaacaacca cactgcaaat
    20281 attgaagcta ctgcaacacc caagtatcct gcccttgaat tgcatcctat ccaaacaaat
    20341 atcatcagca caactgctat tgctgataca attctttgct ttttcgtttt tgctcccaac
    20401 agaaaagcaa tggataacgg aagcataagg gttgcaaaac ttcctacaaa gttcgtgtta
    20461 aacagtgtac cgtaaattgt tttaggtccg aagggaaacg ataaattttc aacatgaagt
    20521 cttccaggta caataagacc tgttcctatt tttgtctgga agaaatcaaa gccaaaatat
    20581 tgtgttatgc ctattaatcc ttctattatc ataagaacta agaatgcttt tacaaacagt
    20641 gttacatccc tttcgctgtt tacaaagttt atagtaagga atgtgattag tacatagctt
    20701 atcagaacaa acataccctg gtacatatca acgaatcccc acagagctgt ttgggtgtct
    20761 atggccaata tggttgatat tattacacaa atcgcataaa tgcccagggg tatatagtat
    20821 tgttttaaat tcttaaatgg cagtttcttt tgtttatata atattatata aagaataagt
    20881 gctattgctg taagaactac tgtccacctt gatttccagt aactgaagaa atccaagtac
    20941 tgtctttggc ctgtccaata tagggcttgg gttgttcctt ccaagtcaat atgctttgca
    21001 tatactataa gcggtacaaa tcctgctatt aataacagag gcaagaagta tgcagcagat
    21061 aattttttgt tttcatagtc tatagctaca ggttgttgtt tttgttttgt ttttatattt
    21121 tttttcttca ctttgtatcc ctcgatattt aagtaaatgt ttagcttctc cgcaagtaag
    21181 tttatgttaa aataacttag aagctggtta agcaagctgt ctaagttatt ataacatgat
    21241 atgaatcaat tgcaaatata aatgtttttg acaattatgg gttatattat tgcctttgcc
    21301 agcttcttaa ctaagtcatc tccatatctg tagtgcctcg ccatatagtc catagttttg
    21361 tctaaaggca agtgctttaa tttaatcatt caacatcact gtctcttata aagtcaatga
    21421 agtcgcccga cttcccgtca tttaaatatt taaggtattt ttcaacagct atatcaagct
    21481 gcatttttgc ataattttca atggcatcaa aggaattttg aacaattttt tcaatgcatt
    21541 tttttgttac aattattttt aagggagaag gaataagttc gtacaaggct tcaacaaccc
    21601 aagaaaactt ttctcctcct gcttttgtac tatc
//
LOCUS       DAOPUE010000026        21595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2423_length_21595_cov_9.971866, whole genome shotgun sequence.
ACCESSION   DAOPUE010000026 DAOPUE010000000
VERSION     DAOPUE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21595
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2423_length_21595_cov_9.971866"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKH42_03875"
     CDS             complement(<1..203)
                     /locus_tag="PKH42_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19603.1"
                     /translation="MYQKSIVHYMTKICIDILFIVAIICCILVPFSSSWLFQYFGIVG
                     ANTIFIKAILLASGISCAYILWQ"
     gene            complement(404..1036)
                     /locus_tag="PKH42_03880"
     CDS             complement(404..1036)
                     /locus_tag="PKH42_03880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOA19604.1"
                     /translation="MLKIEQGLWDKGYNYIACIDEVGRGCLAGSVVTCAIIMPKGLLI
                     EGVNDSKKLTAKKREELFDIIIESSLGYGIGEYDCKKVDEYNIKNATKLAMVQAVENI
                     KDKEGNKIVPDYILVDAEKLDINIPQFNIIHGDAICHGIAAASIIAKVTRDRQMELLH
                     IQYPEYNFSKNKGYGTKEHIEALLEYGPSEIHRKSFVKKILDKNQQLSFL"
     gene            complement(1165..2007)
                     /gene="ylqF"
                     /locus_tag="PKH42_03885"
     CDS             complement(1165..2007)
                     /gene="ylqF"
                     /locus_tag="PKH42_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOA19605.1"
                     /translation="MNINWYPGHMKKTKELLLENIKLVNLIIEVVDARIPYSSKNPDF
                     DILFRDKKRLIVLNKYDLSDSNANKLWENYYRSKGWSVVLYNSTDNKELRKLNKAIAE
                     ATQEIVERYKKRGLLNKTIKAMIVGIPNVGKSTLINSLARKKSAKTGNMPGVTKGKQW
                     IHLAGNIDLLDTPGILWPKFDDEECALNLAFTGAIKDDVLVLEEIALKFVERMQQMGK
                     ENLILERYGIEAGENPLDILNNIAHKKGFLINKTDIDYHRVARLLFNDFRNGKLGNIT
                     LEMP"
     gene            complement(2087..2431)
                     /gene="rplS"
                     /locus_tag="PKH42_03890"
     CDS             complement(2087..2431)
                     /gene="rplS"
                     /locus_tag="PKH42_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOA19606.1"
                     /translation="MDLIKSIENEQVKGVTPFNVGDTIKVHVKVKEGNRERIQIFEGI
                     VIKKQGESNRATFTVRKISYGVGVERTFPVHSPKIEKIEVVRRGKVRRARLFYLRERT
                     GKATKVKELRRY"
     gene            complement(2530..3246)
                     /gene="trmD"
                     /locus_tag="PKH42_03895"
     CDS             complement(2530..3246)
                     /gene="trmD"
                     /locus_tag="PKH42_03895"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOA19607.1"
                     /translation="MKFEIVTLFPELIEVYKKNGIIGRAVENKLFEIEYVYLRDYSED
                     KHKKVDDYPYGGGPGMLLRPEPMFRALEAIKKHNSYIVYLSPKGSLFNQNKAKELSDK
                     EHIILIPGHYEGIDQRIIDKYVDEEISVGDYVLTSGELPALMIIDAVGRLIKGVLGSD
                     ESSKEESHSENLLEYPQYTRPESYMGLDVPEILLSGHHKNIEEWRLFKAIEITLKRRP
                     DMIKDKSIIEKYNELAKKYK"
     gene            complement(3243..3743)
                     /gene="rimM"
                     /locus_tag="PKH42_03900"
     CDS             complement(3243..3743)
                     /gene="rimM"
                     /locus_tag="PKH42_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227124.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOA19608.1"
                     /translation="MKDYIKVGKIINTHGIKGYIKCMPMTDDLERFEELEYVYTEKDN
                     IKRVIDDVWYQKGMVYLKLRNIDDMNTAESYKDTYISILEDQLRELPEDSYYLFDLNG
                     MDVYSSTGEYIGKISEIYQTGANDVYEVRNNKSSFLIPAIKDVVKSVNIQEKKMIIDV
                     IEGLLE"
     gene            complement(3760..3990)
                     /locus_tag="PKH42_03905"
     CDS             complement(3760..3990)
                     /locus_tag="PKH42_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOA19609.1"
                     /translation="MGELVEFIAKSLVDNPDMVEVNEVEGSQSLIIELKVAPDDMGKV
                     IGKQGRIAKAIRTVVKAAATKDNKRVIVEIIQ"
     gene            complement(4002..4265)
                     /gene="rpsP"
                     /locus_tag="PKH42_03910"
     CDS             complement(4002..4265)
                     /gene="rpsP"
                     /locus_tag="PKH42_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOA19610.1"
                     /translation="MSVKIRLKRMGSKKKPFYRIVVADSRSPRDGRFIEEIGYYNPVV
                     EPKVVKINDESAIKWLNNGAKPTDTVSMLFKNNGIYEKRDENK"
     gene            complement(4386..5735)
                     /gene="ffh"
                     /locus_tag="PKH42_03915"
     CDS             complement(4386..5735)
                     /gene="ffh"
                     /locus_tag="PKH42_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOA19611.1"
                     /translation="MFENLSDKLQNALQKLRGKGKLTEKDIDLAMKEIKMSLLEADVN
                     FKVVKAFINNVKERSLGVEVMESLTPAQQVVKIVHEELAKIMGQGESKLKFNSNDITY
                     FMMCGLQGAGKTTATGKLARRLKKDGRRPLLVACDIYRPAAIKQLMVVGESVGVPVFE
                     MGDKKSPVTIAKEAINHARKHGNDVVIIDTAGRLHIDDELMEELVEIKKTVKPDEILL
                     LLDAMTGQDAVKVAETFNSYLDITGVILTKVDGDARGGAALSIRTVVDKPIKFLSVGE
                     KMDQLETFHPDRMASRILGMGDVLSIIEKAQDAFDEKQAIELQKKLKTQDFNLEDFLT
                     QLQQMKNMGPLDEILEMIPGLGGKKKLKGLKVDEKELVYTEAIIQSMTKKERMNPSII
                     NGSRRKRIAAGSGTSVQRVNSLLKQYEEMKKMMKKFQAMGTGKKKGFGGMGGFKLPF"
     gene            complement(5738..6085)
                     /locus_tag="PKH42_03920"
     CDS             complement(5738..6085)
                     /locus_tag="PKH42_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOA19612.1"
                     /translation="MSEKDFIYSILYDYYGDLLKKNQATIIEMYYNLDYSLSEIAEEM
                     NISRQGVHDALKRAEKNLAEYERKIKLYDKYVKFSEAAENIIQLSSKIDDKKYKDVIE
                     KIKLEALRIINEG"
     gene            complement(6389..7306)
                     /gene="ftsY"
                     /locus_tag="PKH42_03925"
     CDS             complement(6389..7306)
                     /gene="ftsY"
                     /locus_tag="PKH42_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOA19613.1"
                     /translation="MNNEKGFFAKLKEGLSKTKKNLTEQIEGVIFQYKKIDDNFLDEL
                     EEILITSDMGVGTTMDIINYIKEEAKKRKLEDTLELKDVIKEYLINVLKENERPDVTD
                     KQRVILIVGVNGVGKTTSIGKIAYRLKQSGNKVIVAAADTFRAAAVEQLKEWCQRAGV
                     DIIASEQGSDPGAVVFDAIQAAKARKADILICDTAGRLHNKKNLMNELSKIFKILDKE
                     FSEAHIDVYLVIDATTGQNGIVQAKLFKEACNINGLILTKLDGTAKGGIAFPIVNELK
                     IPIKYIGVGEQIDDLQDFNAQDFVNAIFE"
     gene            complement(7399..10980)
                     /gene="smc"
                     /locus_tag="PKH42_03930"
     CDS             complement(7399..10980)
                     /gene="smc"
                     /locus_tag="PKH42_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOA19614.1"
                     /translation="MYLKKIYVNGFKSFAEKTEIEVEKGITAIVGPNGSGKSNISDAI
                     KWVLGEQSAKSLRGGKMDDVIFAGTAKRLPLGYAEVRLTFNNESGIIPLEYKEVSISR
                     KLYKTGESEYYINKQQCRLKDIKELFMDTGIGREGYSFIGQGRVEDILSPNSDVRRQV
                     FEEASGIVKYKTRKEESERKLEKTKNNLDRVEDIIYELKERIEPLYEQSIRAQKFIEI
                     RDNLKKYELNYLAHEYEKHCEQLKALKNQRDMSLEEKLRLQKRRDYYAEIIESQKEKI
                     NDLQNKINETEALRDSKNKALEKYKSLRQVHKEKKLLYSSNIDNIKNEIGELEKRNLE
                     LEEKINVLIQEKVSCEEIYKRDIEKFESLNSDIKEYKEEIDEIVQSIEQQKNELFELH
                     KDINKNNSNKNTIESLIINNGDRIKHLNQEVNSEEEERQKRIETINNLQEEQRITREL
                     LIKKKAVLKSILEELNNAEKNKERLNLHISKLNEEYNGTKSKINILTNMEAYYEGYYK
                     SIKTVMNNKDKEPVLNSILGTVADVIKTEKKYEKAVEIALGSAIQNIIVNTDKEAEEI
                     IEYLKKNKIGRVTFLPINMLTERSLNDFEREVLKEECVIDTADNLIQTNSKYEKVIKN
                     LLGRIIIADNLNNGLKISKKLGNTVKIVTLQGDVINAGGSVTGGHISSNQALLGRKRE
                     IDELKAHLDKLSEELKEKNDSLKLLNFKIKELNNSVEEIKSFINEKNNYYEMNNSKIK
                     MLSEQNEKTTAAINKYKEEIHFIQSEKQKYENDLRYLNAETEKLKLLVEQKEKSIEET
                     ILMNNSNKLKYDEYNNTISIQRDKVSNITQNIKLIEEKLNNIHMEIERNKNSIKYKEE
                     SLSSIEQEIEAAREKIIEFTECSDALEEEIKSLNESYIKEKETHKNYQNEIYDYQNKI
                     NESNKLITDILDEEMKLNVKIERESSKIEDISAKLWEDYEINYAMALKYKDDSISQTK
                     LYSEVNSCKKAIKELGNINLDSIEEYKEVRERYDFLTKQEKDLKDAIEQLNEVIKELE
                     IQMKEKFVEEFYNIRAKFSEVFKKLFNGGNGDIYLENEEDALNSNIEITVQPPGKKLS
                     KISLLSGGEKALTAIALLFSILKTKPTPFCILDEIESSLDDLNIFRFSQYLKEFSRET
                     QFIVITHRKGTMEYADTLYGATMEEKGVTKLVSMKLSDEQSIIGTHS"
     gene            complement(10971..12068)
                     /locus_tag="PKH42_03935"
     CDS             complement(10971..12068)
                     /locus_tag="PKH42_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA19615.1"
                     /translation="MNKKIKIIPIFVPHVGCPNNCVFCNQRRITGQKNFTVDGEYVHK
                     IVKRYIETIDGNTNTEIAFFGGSFTAIDTKIQEELLKAAKHYKDLKIISSIRCSTRPD
                     AINDEILMLQKKYGMDIIELGIQSLDDEVLKISNRGHNKKQSIDASRLIKEYGFILGH
                     QIMPGLPGSSRIKDIKTCIESIEMKPDIVRIYPTLVIKDTELFDMYEKGLYKPLSLDE
                     AVEISAYIYSLYSINDISVIRIGLQNTESINNDKDVMAGPFHPAFRQLVEERIYYYAL
                     GSKLCKVNTKELGESITIHAPENLISSIAGQKRSNINKLKERFSIKQISFINKKDDII
                     EIYNKNKRLFSFKESEVFQNYLNMQQGDLCI"
     gene            complement(12061..12768)
                     /gene="rnc"
                     /locus_tag="PKH42_03940"
     CDS             complement(12061..12768)
                     /gene="rnc"
                     /locus_tag="PKH42_03940"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOA19616.1"
                     /translation="MITGTVYEFEKIIGYKFNNIKILEESLTHSSYSNEDKAYNKVNN
                     ERLEFLGDAVLSISVSRYIFDEFPDYPEGDLTKLRAQVVCEDTLSLVAANLNLGKYLL
                     LGKGEESSGGRERKSILADAVEAVIAAIYLDGGYKQAEKFVLNNLTEYIKLAVKGKII
                     TDFKSYLQEYYQSKSQSCKIRYVVTKEEGPDHDKVFHVNVMVNKNVAGKGVGKNKKIA
                     EQNAAKDALIQEGCLNE"
     gene            complement(12863..13099)
                     /gene="acpP"
                     /locus_tag="PKH42_03945"
     CDS             complement(12863..13099)
                     /gene="acpP"
                     /locus_tag="PKH42_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOA19617.1"
                     /translation="MNDVFERIRELVAEKVGVEPEDISMETSFTDDLEADSITLFELV
                     MAIEDEFDIEVDDESIEKIETVGDIVRYLEDIII"
     gene            complement(13096..14094)
                     /gene="plsX"
                     /locus_tag="PKH42_03950"
     CDS             complement(13096..14094)
                     /gene="plsX"
                     /locus_tag="PKH42_03950"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOA19618.1"
                     /translation="MKIAIDAMGGDNAPEAVIIGSIQARDEYNLSLVLCGKEIEIKRI
                     LEKNTSNFNNIEIINAEDIISNDDKPVLAIRRKKESSMVKALYAVKEGNADAIVSAGS
                     TGALLSGGTLLIGRIKGIERPAIGSLIPNMSGGFCLLIDSGANVDSKPEFLYDFAIMG
                     DIYLKRVMNIKSPRIALANIGVEKSKGNKLTVETYELLVNSQLKLNFIGNIEARDILK
                     GEADIVICDGFTGNMILKTMEGTVLEIMKGLKEEIMSSMKTKLGGMLIKPALKNFKRK
                     FDYSEHGGAPLLGVKAPVIKAHGSSDAKAIKNAIRQAKICGESNVNELIEESIMRR"
     gene            complement(14212..14391)
                     /gene="rpmF"
                     /locus_tag="PKH42_03955"
     CDS             complement(14212..14391)
                     /gene="rpmF"
                     /locus_tag="PKH42_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOA19619.1"
                     /translation="MAVPKRKTSKAKRDSRRAANAKLTVPNTVECPQCHEPKLPHRVC
                     ISCGYYDGKEVVAVE"
     gene            complement(14404..14898)
                     /locus_tag="PKH42_03960"
     CDS             complement(14404..14898)
                     /locus_tag="PKH42_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOA19620.1"
                     /translation="MLLKINKFLSSDNVSMTVNENFVIDDEEFLEETRLDKNILLSGE
                     IFKIDDSLTFSGRIEYTLTDECARCLKEFNNRINYKFQASLVHKEDKESDEVQMVITD
                     GIIKMDDIIKQLIYLSLPMKSLCDKDCRGICPNCGANLNNEKCQCVNSLTDPRFDKLK
                     DLLI"
     gene            complement(14971..16167)
                     /locus_tag="PKH42_03965"
     CDS             complement(14971..16167)
                     /locus_tag="PKH42_03965"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOA19621.1"
                     /translation="MNVLVINCGSSSLKYQLINMDDESVLAKGLVERIGIDGSLLKHE
                     RTGLDKLVIEEDLKNHKDAVNLMLRALLDEKYGAVKSLDEIDAIGHRVVHGGEKFACS
                     VVINDEVIDTMKENIGLAPLHNPPNIIGIEACKELLPDVPMAGVFDTAFHQTMPPVSF
                     IYPLPYELYEEMRIRRYGFHGTSHKYVSERVAEITGKSLEGTKIVTCHLGNGASLTAI
                     KDGKSFDTSMGMTPLEGLVMGTRCGDIDPAIVTYLICKKDMTAEQVDNLMNKKSGVLG
                     ISGVSNDFRDIEAEADKGNKRARLALDKFAYTVKKYIGSYAAAMGGLDYIVFTAGLGE
                     NSATARKEICEGLEFLGVEIDDEKNKIRGKEAEISKDGSRVKVFVIPTNEELMIARET
                     KELTSK"
     gene            complement(16224..16799)
                     /locus_tag="PKH42_03970"
     CDS             complement(16224..16799)
                     /locus_tag="PKH42_03970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOA19622.1"
                     /translation="MIYLVRHGESESNISRRFSGITDVELTETGALQAVKAGKKLRGK
                     TIDNIFSSPLKRAKNTAEIIADEIGFNKKDIIIENRLTEVNFGIFENLTWEEILEQYA
                     DETERWIEFKHKYKFPKGEGYDDIIYRVSGFIDNVPDNSLILTHYGVIQGILLYYKIV
                     DDNTLWNYQISNCDIFILNNKKLENIIKCVH"
     gene            complement(16796..17533)
                     /gene="cobS"
                     /locus_tag="PKH42_03975"
     CDS             complement(16796..17533)
                     /gene="cobS"
                     /locus_tag="PKH42_03975"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOA19623.1"
                     /translation="MASGFLILISFFTRIPVGEKIEYNEENFVKALNLFTLVGVVIGF
                     LLAVVYLIFNNQISFIRGLVITVMYILISGGIHLDGACDTADGLLSGRTGDRIFEIMS
                     DSHIGAFGVIALILIILSQFVLFSFCSIETVFMMPLVGRASTIVSCWNKKYAKKIKGM
                     GTVFIENIDKNVLLINLLILFVFSMIPWYRAIMFTACFAAIAVSYFISILIEEKIGGM
                     TGDTCGFITEISQIVFMTIVLFLMRQI"
     gene            complement(17515..18108)
                     /gene="cobU"
                     /locus_tag="PKH42_03980"
     CDS             complement(17515..18108)
                     /gene="cobU"
                     /locus_tag="PKH42_03980"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOA19624.1"
                     /translation="MSLIFVIGGARSGKSTFAELKAKEYSENVLYIATAAVTDEAMAD
                     RVKKHREQRPAFWQTLEMYSDFKTLINKAEFNQSEVVLLDCITTMIGNYMVESKLDFD
                     DCSVEDVNILENKITEEVFSLINVCKENNKKLIIVSNETGMGLVPSYYMGNYFRDISG
                     RINNKIGVQAEHMYFIFSGIPIKLKHKGEMIKWPQDF"
     gene            18333..19532
                     /locus_tag="PKH42_03985"
     CDS             18333..19532
                     /locus_tag="PKH42_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOA19625.1"
                     /translation="MRITGVVAEYNPFHSGHEYQLRMARRLSGCDCIAVVMSGNYVQR
                     GEPAIIDKFKRAEAAIYGGADIVIELPMPFSCQNAEMFAHAAIMELKKLNVDSISFGC
                     ETDNVRLLKKIALLQLTPGFNNYIKEEMKKGLSYPNAMSNALKFILNEEAEAVYSPNN
                     VLAVEYIKSAVKLNLELQYYPVKRVGMAHNDTEISGLFHSATAIRNAIMEGSFDNLKL
                     TEKSIELIKKFCKEHKKYNILNNYRDYLYYKIITCDDLEEIYEINEGLNNKIISQVYK
                     HETIDEFIMSLKSKRYTYSKLRRSLLNILLGIKKVDIINIMSTNDKYVKVLAFNNTGR
                     KAIKNAVNNGTIVINRYSDYKKNNLTPEKLMRFNITNKTSNIYYLPLENRKMNSEYFE
                     NAVYVDV"
     gene            19638..20588
                     /locus_tag="PKH42_03990"
     CDS             19638..20588
                     /locus_tag="PKH42_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19626.1"
                     /translation="MLRRNFIPVFIILIICLFVMKPGIVSEGVFNGLKIWVNNMIPYL
                     LPMSILSNILLQYNFLYRISEKLSFLSNKIFNSKYAFIPYFISFVVGYPSGAMTVNTM
                     ASFKRIDSSEANALMVFTNNCSFQFMAGAVSFSMLGDLKLAKYIAIPHLLGALLIGII
                     IKKGSISTAFTKEKKHKYISFYDAFNSSIYKSITSILSIGGVIVIFSVFSNFFDNLLI
                     SVSKYLPLSINLSDIIHSVLTGILEISNGCSVTASSVLPLDVKLIIINFLISFSGISV
                     IFQTIAVANDFDFKISNYVFYRAIQGILSVIICIGMLILS"
     gene            20641..21549
                     /locus_tag="PKH42_03995"
     CDS             20641..21549
                     /locus_tag="PKH42_03995"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOA19627.1"
                     /translation="MINVTLIGTGGMVPLPGRFLASCHIDYQGKAILIDCGEGTQISL
                     HKGKISLNKIDTILITHCHADHVTGLPGLLLTMGNHGRTNHIDIIAPRGSTKILNSLL
                     MVCGFLPFEVRIAELHDTKPQKFSKIGLNITSIPLKHHINCLGYSFELILRPHFQPEK
                     AKKLNIPVKLWRLLHGGNEVVVDDRTITPDMVLGSPRKPIKISYVTDTRPVKLIDEII
                     KDSDLFICEGMYGNDEMFEGAVAKMHMIFSEAANISLNANVKELWLTHFSPSMPNPEE
                     YKEFAENIFSNTVIGKDLMSKTLSSP"
BASE COUNT         6108 a         4241 c         2983 g         8263 t
ORIGIN      
        1 aactgccata aaatatatgc acaagaaata cctgaagcta ataatattgc tttaatgaaa
       61 atagtatttg ccccaactat tccaaaatac tgaaacagcc aagacgaaga aaatggaacc
      121 aaaatacagc aaataattgc cactataaac aaaatatcaa tgcaaatttt agtcatataa
      181 tgaactatgc ttttctggta cataaaatcc tccaaaattt attattgtat tctaattata
      241 gcagatattt tttgcatgtc aataaatttt tattgttttt cgataatatt ttattgataa
      301 ataaaaataa ggcatcgtga ctctcaatgc cttatatttc tatctgtatt tctaattcat
      361 tccaataact tcatttttac ttaaataaaa aacgtcgccg atattatagg aaactcagct
      421 gctggttctt atcaagaatt tttttaacaa atgattttct atgtatttcg gaaggaccgt
      481 attcaagcag ggcttcaatg tgctcttttg taccataccc cttattttta ctaaaattat
      541 attccggata ttgaatatgt aagagctcca tttgtcggtc acgggttact tttgcaatta
      601 ttgatgctgc tgctattccg tgacaaattg catctccgtg gattatatta aactgcggta
      661 tatttatgtc aagcttttcg gcatcaacaa gaatataatc aggaactatt ttgtttcctt
      721 ctttgtcttt tatgttttcg acagcttgta ccattgcaag ctttgttgca tttttaatat
      781 tatattcatc aaccttttta cagtcgtatt ctccaatacc gtatcccaaa gatgattcaa
      841 taattatatc aaataattct tctctttttt tagccgtcag ttttttggaa tcattaacgc
      901 cttctatcag taaacccttg ggcataataa ttgcacaggt tacaacgctg cctgctaaac
      961 agcctctccc tacttcatct atgcatgcta tgtaattata tcccttgtcc cacagacctt
     1021 gttcaatttt aagcatgtta ttctccttta gggcatctcg cagtctcatt caccaaaata
     1081 tttgctccct ttgctcgcat attttgggaa ttcgttgcga atgacctacc atccattttt
     1141 ctgaatacaa tcagaaaaat ggggttaggg catctctaat gttatgtttc ctaattttcc
     1201 gtttctgaag tcgttaaata ataatcttgc tactctatgg tagtctatgt ctgttttatt
     1261 tattaaaaag ccttttttat gggctatatt gttcaaaata tccaaagggt tttcccctgc
     1321 ttctatgccg tatctttcaa gaattaaatt ttcctttccc atttgctgca ttctttcgac
     1381 aaacttaaga gcaatttcct ccaataccaa tacatcatcc tttattgcac ccgtgaatgc
     1441 aagatttaaa gcacactctt catcatcaaa tttcggccac aatattccgg gagtatccaa
     1501 taaatcaata ttaccggcca agtgtatcca ttgtttgccc tttgttacac cgggcatatt
     1561 tcccgtcttt gcgctcttct ttcttgccaa tgaatttatt agtgttgatt tgcctacatt
     1621 tggtatgccg acaatcattg ctttgattgt tttgttcaat aatccccttt ttttatatct
     1681 ttcaacaatt tcttgagttg cttctgctat tgccttattc agtttccgaa gctctttatt
     1741 gtctgttgaa ttataaagca caaccgacca gcccttgctt ctgtagtagt tttcccataa
     1801 tttattcgca tttgagtctg ataagtcgta tttatttaat acaatcagtc tttttttgtc
     1861 tctaaacaat atgtcgaagt caggattttt actgctgtat ggaattcttg catctacaac
     1921 ttcaatgatt aaattaacta atttaatatt ttccagcaat aactcctttg tctttttcat
     1981 atgtcccgga taccagttta tattcataat gtacctctaa tctgaaaggg acacacctta
     2041 ttaaaggaat gtccccttta tatgaggacg tccgccctca attcaattaa taccttctca
     2101 gttctttaac tttagttgct ttacctgttc tttctctcaa gtagaacaaa cgagcacgtc
     2161 ttactttacc tcttcttacc acttcaattt tttcaatctt tggtgaatga acagggaatg
     2221 ttctttcaac tccaacgccg taagatattt ttcttaccgt aaaagtcgct ctgttgcttt
     2281 cgccttgttt tttaattacg atgccttcaa atatctgaat tctttctctg tttccttctt
     2341 tgacctttac atgtactttt atagtatctc caacattaaa aggtgttacg cctttaactt
     2401 gttcgttttc tatagatttt attaagtcca tttgtatacc tcccttcgcc ttaacgttct
     2461 tgttacctgt acagaggacc gccgtatact tttagcatta tagcatatga atcataatgc
     2521 atacaacatt tatttatact tctttgctaa ctcgttgtac ttttcaatta tacttttgtc
     2581 ctttatcata tcgggacgtc tttttaaagt aatttctatt gctttaaaca aacgccattc
     2641 ttcaatattt ttgtgatgtc ccgataataa tatttcgggc acatctaagc ccatgtagct
     2701 ttccggtctt gtgtattggg gatattcgag taaattttcg ctatgtgatt cttcctttga
     2761 agattcatca gagcccaata ctcctttaat gagccttcct accgcatcaa taatcattaa
     2821 tgcaggcagt tccccgcttg ttaagacata gtctcccaca gatatttcct catcaacata
     2881 tttgtcgata attctttggt ctataccttc ataatgtccg ggaataagta ttatatgctc
     2941 cttatccgaa agctctttag ctttattctg attaaataaa ctgccttttg gtgataaata
     3001 aacaatatat gaattatgct ttttaatagc ttccaaagct ctaaacatag gttccggtct
     3061 caatagcata ccgggacctc cgccataagg ataatcatct acttttttat gtttatcttc
     3121 ggaataatcc ctaaggtaca catattctat ttcaaacaat ttgttttcta cagctcttcc
     3181 gattattccg ttttttttat aaacttcaat taactccgga aataaagtta ctatttcaaa
     3241 tttcattcca ataacccttc aataacatca attatcattt ttttttcctg aatattaact
     3301 gatttcacaa catcctttat tgcaggtatc aaaaatgagc ttttgttatt tcttacttca
     3361 tatacatcgt tggctccggt ttgatagatt tctgaaattt ttccgatata ttcaccggta
     3421 ctagaataca catccattcc attcaaatca aataaatagt aggaatcttc gggaagttct
     3481 ctcagctggt cctcaagtat ggaaatgtat gtgtccttat atgattctgc agtattcata
     3541 tcgtcgatat ttcttagctt aagataaacc ataccttttt ggtaccatac atcgtcaatt
     3601 acacgcttta tgttgtcttt ttccgtgtag acgtattcta actcctcgaa tctttccaaa
     3661 tcatcggtca ttggcatgca ttttatatag cctttgatac cgtgtgtatt tattattttg
     3721 ccaactttaa tataatcctt catttctacc tcagaaaggt tattgaatta tttctactat
     3781 aaccctttta ttatcctttg ttgcagctgc tttaacaaca gtccttattg cttttgctat
     3841 tctgccttgt tttcctatta ctttacccat atcatcagga gctactttta attcaattat
     3901 cagtgattga ctgccttcaa cttcattgac ttcaaccata tcgggattgt caacaagaga
     3961 ttttgctata aattctacta attcacccat gatatcacct cttatttgtt ttcatctctt
     4021 ttttcgtaaa tgccgttatt tttgaaaagc atacttactg tgtcggtcgg tttagctccg
     4081 ttattcagcc atttgattgc actttcatcg tttattttca caaccttcgg ctcaactaca
     4141 ggattgtagt atcctatttc ttcaatgaaa cgaccatctc ttggagagcg tgaatctgct
     4201 actactattc tgtaaaaagg tttctttttg gaacccattc tttttaatct gatttttact
     4261 gacattatgt cacctccttg atttagtgtg tgggacacac ctctttttat ggagctatgc
     4321 tcctgtttga ggaataccct gaatatatat gaaaaaggaa aatcctattt ctaacaataa
     4381 tatggttaaa atggcagctt gaatccgccc ataccgccaa agcctttctt cttgcctgta
     4441 cccatcgctt ggaatttttt catcattttt ttcatttctt catactgctt taataatgaa
     4501 ttgacccttt gaacagatgt tccgcttccc gcagctattc tttttcttct gctgccgttg
     4561 attatcgaag gattcattct ttccttctta gtcatagatt gaataattgc ttccgtatac
     4621 actaattctt tttcatcaac tttaagacct tttaattttt tcttgcctcc aagtccggga
     4681 atcatttcca atatctcatc caaaggtccc atatttttca tctgctgaag ctgcgttaag
     4741 aaatcttcta aattgaaatc ttgagttttt aatttctttt gaagttcaat tgcctgtttc
     4801 tcatcaaaag catcctgggc tttttcaata attgatagaa catctcccat cccgaggatt
     4861 cttgatgcca ttctgtcagg atgaaatgtt tcaagctggt ccatcttctc tccaacagat
     4921 aaaaatttta taggcttatc cactacggtt cttatggaaa gcgctgctcc gcctcttgca
     4981 tcaccatcaa cctttgtaag tattactcct gtaatatcca aataagaatt gaatgtttct
     5041 gcaactttaa ctgcatcctg cccagtcatc gcatccaata acaaaagtat ttcatcaggc
     5101 tttacagttt ttttaatttc aactaattct tccatcaact catcatctat atgaagccgt
     5161 ccggcagtat ctataataac aacgtcgttt ccatgttttc tggcatgatt tattgcttcc
     5221 tttgcaatag taacaggaga ttttttatct cccatttcaa aaacaggaac tcctacactt
     5281 tctcccacta ccataagctg ctttattgca gccggtctgt atatgtcgca agctacaagt
     5341 aaaggtctcc tgccgtcttt tttcagtctt cttgcaagct ttccggtagc tgttgttttt
     5401 cctgcccctt gaagcccgca catcataaaa tatgttatat cattcgaatt gaatttaagc
     5461 ttgctttcac cctgacccat tattttggct aattcttcat ggactatttt tacaacctgt
     5521 tgtgcgggag tcaaactttc cattacttca acgcctaatg acctttcttt cacattgtta
     5581 ataaaggctt taacaacctt aaaattaaca tctgcttcaa gaagtgacat tttgatttct
     5641 ttcattgcca agtctatatc tttttcagtc agttttccct tgcctctaag cttttgtaat
     5701 gcattttgaa gtttatcgga taagttttca aacattttta accctcatta attattctta
     5761 atgcttcaag ttttattttt tcaataacat ctttatattt tttatcatca atctttgatg
     5821 ataattgtat gatgttttct gctgcttcac tgaatttaac gtatttatca tatagtttta
     5881 ttttccgctc atactctgca aggttttttt cagccctttt taaagcatca tgtactccct
     5941 gcctgctgat gttcatttct tcagcaattt ctgataagct gtaatccaag ttgtaataca
     6001 tttcaattat ggttgcttga ttttttttaa gcagatctcc ataataatca tacaagatgc
     6061 tatatataaa atccttctct gacataataa cccctgtaaa gtattttact tgacagtgaa
     6121 ttttaccgca tcctgattat tttgtcaata gtttttttga tatattgaga catattattc
     6181 attataatga aaaaaataat aaaaatatca agcgaacatt tgcaggacac aattataatt
     6241 cttagcgaat gaaggcttaa aacccgttaa gcagcttgca ctgtttgagc agagcgagtt
     6301 tgcaagctgt agggttttta gccgtagtga gcgcatagaa ttattcgtgt ccgaaaccgt
     6361 gagcggatat ttttattatt tttcgtgttt actcaaaaat tgcattaacg aaatcttgag
     6421 cgttaaaatc ctgcaggtca tctatttgct ctccgacacc gatatatttt atcggtattt
     6481 tcagttcgtt tacaatagga aatgctatgc ctccctttgc tgttccatcc aatttcgtta
     6541 aaatcaaacc gttaatattg catgcttctt taaacagctt tgcttgaaca ataccatttt
     6601 gaccagttgt tgcatctatt acaagataca catcaatatg ggcttcgctg aattctttat
     6661 caagaatttt aaaaatcttg ctgagctcat tcataaggtt cttcttgtta tgaagtcttc
     6721 ctgcagtatc acatatcaat atatctgctt ttcttgcttt tgctgcctgt attgcatcaa
     6781 aaacaactgc tccggggtct gagccttgct ctgaagcaat tatatcaact cctgctcttt
     6841 gacaccactc tttaagctgc tcaaccgctg cagctctaaa tgtatctgca gcagcaacga
     6901 taaccttgtt gccggactgc tttaatctat aggcaatttt gccgatagaa gttgttttac
     6961 cgacaccatt taccccaact attaatatta ccctctgttt atctgttaca tcaggccttt
     7021 cattttcctt aagaacattt atcagatact ctttaattac atctttaagc tccagcgtat
     7081 cttctaattt tctctttttt gcttcttctt tgatataatt aataatgtcc atcgttgttc
     7141 caacacccat gtcggaagta ataagtattt cctccaattc gtcaagaaaa ttatcatcta
     7201 tttttttgta ttgaaaaatc acgccttcaa tttgctctgt aaggtttttc tttgttttgg
     7261 aaagtccttc ttttaatttg gcaaaaaagc ctttttcatt gttcatattt gcctccatct
     7321 tatttgggga cattcttctg tccctcttcc gctatctctt tagcgactat ccctatccga
     7381 gtggtttatc cccatagctt aactgtgtgt ccctataatg ctttgttcat ctgacagttt
     7441 catcgatact aattttgtaa ctcccttttc ttccattgtt gctccgtata aagtgtcagc
     7501 atactccata gttcctttcc tgtgagtaat tacaataaat tgtgtttccc ttgaaaattc
     7561 ttttagatat tgcgaaaatc tgaaaatatt taaatcgtca agcgatgatt caatttcatc
     7621 taatatgcaa aatggcgtcg gcttggtttt taaaattgaa aacaagagag caattgcagt
     7681 aagcgccttt tctccgcctg acagcaatga tattttgctc aattttttac ccggcggctg
     7741 tactgtaatt tctatgttgc tgtttaacgc atcttcctca ttttccaaat aaatatctcc
     7801 atttcctccg ttgaaaagct tcttgaatac ttcagagaat tttgctctta tattgtaaaa
     7861 ttcttcaaca aacttttctt tcatttgaat ttcaagctct tttattacct cattcaactg
     7921 ctcgatggca tcttttaaat ccttctcttg ttttgttaaa aaatcatatc tttctctaac
     7981 ttctttgtac tcttctatgg agtccaaatt aatattcccc aattctttaa ttgccttttt
     8041 gcatgagttc acttcgctgt aaagtttcgt ttgactaatg ctgtcatcct tatattttag
     8101 tgccattgca tagtttattt catagtcttc ccatagtttt gcagaaatat cttcaatttt
     8161 tgaactttct ctttcaattt taacattcaa tttcatttct tcatcaagta tatcggttat
     8221 cagtttattt gattcattaa ttttattttg gtagtcatat atttcatttt gataattttt
     8281 atgagtttct ttttctttta tatagctttc gtttaatgat tttatttctt cttccaaagc
     8341 atcagagcat tccgtaaatt caataatttt ttctcttgct gcttcaattt cctgctcaat
     8401 agaggataag ctttcttctt tatattttat ggaatttttg tttctttcta tttccatatg
     8461 aatattattc agtttttctt ctataagttt aatattctga gtaatattag ataccttgtc
     8521 cctttgtatt gaaattgtat tattgtattc atcatatttc aatttattgg aattattcat
     8581 taatatggtt tcttcaatac tcttttcttt ttgctcgacc aataatttca gtttttctgt
     8641 ttcagcattc aaatatctta aatcattttc atatttttgt ttttcactct gtatgaaatg
     8701 aatttcttct ttatatttgt tgatagcagc agtagttttt tcattttgct ctgacagcat
     8761 tttaattttt gaattattca tctcataata attatttttt tcattaataa aactcttaat
     8821 ttcttctacg gaattgttta attcttttat tttaaaattc aaaagcttca atgaatcatt
     8881 tttttctttt aattcttccg atagtttgtc taaatgagct tttaattcat caatttctct
     8941 tttccttccc aagagagctt ggtttgagct tatatgccct cctgtaacgg aaccgcccgc
     9001 atttattaca tctccttgaa gtgttacaat tttaactgta tttccaagct tcttggatat
     9061 ttttagaccg ttgttcaggt tgtctgcaat aattattctt ccaagcaaat ttttaatgac
     9121 tttttcatat tttgaatttg tttggattaa attgtctgcc gtgtcaatta cgcattcttc
     9181 ctttagtact tctctttcga agtcatttaa acttctttct gtaagcatat taataggaag
     9241 aaaagtaact cttccgattt tattcttctt taaatactct attatttctt cggcttcttt
     9301 atcagtgttt acgattatgt tttgtattgc ggagcccaat gctatttcaa cagctttttc
     9361 atattttttt tctgttttta ttacatcggc caccgttccc aatatactgt ttaatacagg
     9421 ctccttatcc ttgttgttca ttacggtttt tatgctttta taatatccct cataatatgc
     9481 ttccatattt gtcaggatat ttatttttga ttttgtgccg ttatactctt catttaactt
     9541 ggaaatatgt aaatttaacc tttctttatt tttttctgca ttatttaatt cttccaatat
     9601 gcttttcagt actgctttct tttttatcag cagttctctt gtaatccttt gttcttcctg
     9661 cagattgttt attgtttcta ttctcttttg tctttcttct tcttctgaat ttacttcttg
     9721 gtttaaatgc ttaattctgt ctccgttgtt tattattaat gattcaattg tgtttttatt
     9781 tgaattgttt ttattgatat ccttgtgcag ttcaaacaat tcattttttt gctgctcaat
     9841 cgattgcacg atttcatcaa tttcttcttt atattcttta atatctgagt ttaatgattc
     9901 aaatttttct atatctctct tgtatatctc ttcgcaagaa actttttctt gaattaaaac
     9961 attaatcttt tcttctaatt ccaagttcct tttttcaagt tctcctattt catttttaat
    10021 attgtctatg tttgagctgt acaacaattt tttttctttg tgtacttgtc ttaagctctt
    10081 atatttttct aaagctttat tttttgaatc tctcagcgct tcagtttcat tgatcttatt
    10141 ctgtaagtca ttaattttct ctttttgaga ttcaataatt tcagcataat aatctcttct
    10201 tttttgcagc ctaagctttt cttccaatga catatctctt tggtttttca gggctttaag
    10261 ctgctcacaa tgtttttcgt attcatgagc taaataatta agctcgtatt tttttagatt
    10321 gtcccttatt tcaataaatt tttgcgcccg gatactttgc tcataaagag gctcgatacg
    10381 ctccttaagc tcatatatta tatcttctac tctatccaga ttattctttg ttttttcaag
    10441 tttcctttca gattcttctt ttcttgtctt gtattttact attccggaag cttcctcaaa
    10501 aacttgtctt ctcacatcag aattagggct taaaatgtct tccacccttc cttgtccaat
    10561 aaaagaatag ccttctcttc ctattccggt atccataaaa agctctttta tatctttaag
    10621 cctgcattgc tgtttgttta tgtaatattc gctttcacct gtcttgtaca gcttcctgct
    10681 gatgcttact tctttgtatt caagtggtat tatacccgat tcattgttga aagtcagcct
    10741 tacctcagca taacccaggg gcagtctttt tgcagttccg gcaaatataa catcatccat
    10801 tttgcctcct ctgagggatt ttgcactttg ctcccccaaa acccacttaa tggcatcgga
    10861 tatgttgctt ttaccgctcc cgtttggtcc tacaatggca gtaatacctt tttctacttc
    10921 tatttcagtt ttttccgcaa aggatttaaa cccgtttaca tatatcttct ttaaatacat
    10981 aaatccccct gttgcatatt taaataattt tgaaatactt ctgattcctt aaaactgaat
    11041 aatcttttat ttttattata tatttcaatt atatcatctt ttttatttat aaaggatatt
    11101 tgttttattg aaaatctttc tttcaattta ttgatattag accttttttg gccagcgata
    11161 ctgcttatca gattttcagg tgcatgaatt gtgatacttt ccccaagctc cttagtattc
    11221 actttgcaca gttttgaccc caatgcataa tagtaaattc tttcttctac caattgcctg
    11281 aaagcgggat gaaagggccc tgccataaca tctttatcat tattgatgga ttcagtattt
    11341 tgaagtccta tccttataac acttatatcg ttgatactgt ataagctgta tatatatgct
    11401 gatatttcta ctgcttcatc cagagataga ggtttatata agcctttttc atacatgtca
    11461 aaaagctcag tatcctttat taccaatgta ggataaattc tcacaatatc cggtttcatt
    11521 tcgattgact caatacatgt ttttatgtcc tttatcctgc tgcttccggg aagtccgggc
    11581 attatctggt gtcctaatat aaatccatac tcctttatga ggcggcttgc atctattgat
    11641 tgttttttat tatgccctcg gtttgaaatc ttcaaaacct catcatcgag actttgtatt
    11701 cctaactcaa ttatatccat tccgtatttt ttttgaagca tcagtatttc atcgtttatg
    11761 gcatcaggtc tcgtggagca tcttatactg cttatgattt ttaagtcctt ataatgtttt
    11821 gcagccttta ataattcttc ctgaattttc gtatcgattg ccgtaaatga gccgccgaaa
    11881 aatgcaattt ccgtattggt attaccatca attgtttcta tatacctttt aacaatttta
    11941 tgaacatatt ctccatcaac agtaaaattt ttttgtcctg taattcttct ttggttgcaa
    12001 aatacacagt tattgggaca acctacatga ggtacaaaaa taggaataat ttttattttc
    12061 ttattcattc aaacagcctt cttgtataag agcatccttg gcggcatttt gctcagctat
    12121 tttcttgttt ttaccgacgc cttttcccgc tacatttttg ttaaccatca cattcacatg
    12181 aaaaacttta tcgtggtcag gcccttcttc ctttgtaact acatatctga ttttgcatga
    12241 ctggctttta ctttgatagt attcctgaag atacgattta aaatccgtta taatttttcc
    12301 tttaacagca agcttaatat actcagttaa attattcaat acgaattttt cagcctgctt
    12361 gtatcctccg tctaaatata ttgcagcaat aacagcttcc acagcatctg ccagtattga
    12421 ttttctttcc ctgcctccgg aggattcttc acctttccct aatagtaagt atttccctaa
    12481 gtttaaattt gccgcaacca agcttagggt atcttcgcaa acaacctggg ctctcaattt
    12541 tgttaagtct ccttcaggat aatcaggaaa ttcatcaaaa atatatctgc ttacggaaat
    12601 gcttaagact gcatctccca aaaattcaag tctttcatta tttaccttgt tatatgcctt
    12661 atcctcattt gaatatgagc tgtgtgtcaa ggattcttcc aatattttta tgttattaaa
    12721 tttataacct attatttttt caaattcata aactgtacct gtaatcaatt ttccctccgg
    12781 aataataaca tatcattgct actggatgac ataactgatg tctgctttca aactatggga
    12841 cgcattgctg cgtccccctt ctttaaataa ttatatcttc caagtatctg acaatatctc
    12901 ctactgtctc tattttttca atactttcat catcaacttc aatgtcgaat tcgtcttcaa
    12961 ttgccataac caattcaaaa agtgttattg aatcggcttc taaatcgtct gtaaaggatg
    13021 tttccataga aatgtcttca ggctcaactc caaccttttc ggctactaat tccctaattc
    13081 tttcaaacac atcattcacc tcctcataat gctttcttca attaattcat ttacattgct
    13141 ttctccgcaa atttttgcct gacgaattgc atttttaatt gcttttgcat cagagctgcc
    13201 gtgtgctttt attacgggag ctttaactcc caacagcgga gccccgccat gctctgaata
    13261 atcaaatttt cttttgaagt tcttaagtgc cggtttaatt aacataccgc ctagtttagt
    13321 tttcatgctt gacataattt cttctttcaa gcccttcata atttccagca ccgtaccctc
    13381 catagttttt aatatcatat tccccgtaaa cccgtcacat attacaatat cagcttctcc
    13441 tttaagtata tccctggctt caatgttgcc gatgaaatta agcttaagct gtgaatttac
    13501 aagcagttca tatgtttcaa cagtcagttt gtttcccttg gatttttcaa ctcctatatt
    13561 tgctaaggca atccttgggg atttgatatt cataactctt ttaaggtaaa tatctcccat
    13621 aatggcaaag tcatataaaa attccggttt tgaatccaca tttgcaccgg agtctatcaa
    13681 taagcaaaac ccacctgaca tattgggtat caatgagcct atcgcgggtc tttcaattcc
    13741 tttaatcctt cctatcagaa gggtcccgcc gcttaataag gctcctgtgc ttcctgcaga
    13801 tactattgca tcagcgttgc cttccttcac cgcatataaa gccttgacca tcgaagattc
    13861 tttcttgcgc cttattgcta agacaggttt gtcatcattt gatattatat cctctgcatt
    13921 aatgatttct atattattga agtttgatgt gtttttttct agtattcttt ttatttcaat
    13981 ttcttttccg catagtacca agcttagatt atactcatct ctagcttgta tacttccgat
    14041 tataacagcc tcaggtgcat tgtctccacc catggcatca attgcaattt tcatattcac
    14101 ctcttatcta atatacttat atcagtatat agcagtaata tttgttttgc aataaaaaaa
    14161 tagtgccaag cactattttt ttaaataccc gacacgggtt ttagagtctt attattcaac
    14221 agctaccact tctttaccgt cataatatcc gcatgatata catactctgt gaggtagctt
    14281 tggctcatga cattgtggac attccacagt attaggaaca gttaattttg cattggctgc
    14341 tcttcttgaa tctcttttgg ctttggaagt ttttctctta ggtactgcca tttttacacc
    14401 tccttatatc aacaggtctt tcaacttatc aaatcgcggg tctgttaagc tgtttacgca
    14461 ttgacatttt tcattgttta agtttgctcc gcaattggga catatgcctc tacagtcttt
    14521 atcacacaga gatttcatgg gtaaggataa ataaatcaat tgtttgatta tatcatccat
    14581 ttttataatt ccgtctgtta taaccatttg aacttcatca gattccttat cctctttatg
    14641 tacaagagat gcttggaatt tgtaattgat tctattatta aactccttta aacatcttgc
    14701 acattcatct gtcagagtat attctattct gcctgaaaat gtcagactgt cgtcaatttt
    14761 gaatatttct ccgcttaata aaatattttt gtccagacgt gtttcttcca aaaattcctc
    14821 atcatctatt acaaaattct cgtttacagt catagacaca ttatcagatg ataaaaactt
    14881 gtttatctta agcaacataa taccaccctt tttaattcgc agagtttatt atatgagaaa
    14941 agaaaatatt tgtcaaggta atatttctta ttatttgctt gttaattcct ttgtttctct
    15001 tgcaatcatc aattcttcgt ttgtaggaat tacaaatact tttactctgg agccatcctt
    15061 tgatatttcg gcttcttttc ctcgtatttt gttcttttca tcatctattt caactccaag
    15121 aaattccaag ccttcgcata tctcttttct tgcagttgcg gaattttcac ctaaacccgc
    15181 agtaaatacg atataatcca atcctcccat tgctgctgca taagagccga tatatttttt
    15241 aactgtatat gcaaatttgt ctaaggccaa cctggctctc ttatttcctt tatcagcttc
    15301 ggcctcaata tctctaaaat cgtttgaaac tcctgaaata cccaaaactc cggacttttt
    15361 attcattaaa ttgtcaacct gttcagccgt catatctttc ttacagatta aatatgttac
    15421 tattgcaggg tctatgtctc cgcatctcgt tcccattacc aaaccttcaa gaggagtcat
    15481 tcccatactt gtatcaaatg attttccatc ctttattgca gttaagcttg ctccgtttcc
    15541 caaatgacat gtaactattt tggttccttc aaggctcttg cctgttattt cagcaactct
    15601 ttcagaaaca tatttatgcg atgttccgtg gaatccgtat cttcttattc tcatttcttc
    15661 atataattca taaggcaatg gatatataaa ggatactgga ggcattgtct gatgaaatgc
    15721 agtatcaaat actcctgcca tcggtacgtc aggcaataat tccttgcatg cttcaatacc
    15781 tatgatgttt ggaggattgt gaagaggtgc caatccaata ttttctttca ttgtatcaat
    15841 aacttcatcg ttaattacta ctgaacatgc aaatttttct ccgccgtgaa ctaccctgtg
    15901 tccgatagca tcaatttcgt ccaatgattt tactgctccg tacttttcat ctaacagtgc
    15961 cctcaacata aggttgactg catccttgtg attttttaaa tcttcctcaa taaccagttt
    16021 gtctaatcct gttctttcat gttttaataa gctgccgtcg attcctattc tttcaactaa
    16081 gcctttagct aaaacacttt catcatccat gtttatcaac tggtatttta atgatgaact
    16141 cccgcaatta attactaata cattcatttt attcctccat agttttaaaa taataataat
    16201 aatatctaaa atcccttaag attttaatga acacatttaa taatattttc tagttttttg
    16261 ttgtttaaaa taaatatatc acagtttgat atttgataat tccacagtgt attatcatca
    16321 actattttat aatacaatag tatcccctgg attacaccgt aatgagtcaa gattaatgaa
    16381 ttgtcaggca cattgtctat aaagcctgaa accctataaa ttatatcatc ataaccttca
    16441 cctttgggaa atttatattt atgtttaaat tcaatccatc tttctgtttc atctgcatat
    16501 tgctctaata tttcctccca ggtcaaattt tcaaaaatgc caaagttcac ttctgttaaa
    16561 cggttttcaa taattatatc ttttttattg aaacctattt cgtctgctat tatttccgct
    16621 gtattttttg ctctttttaa ggggcttgaa aaaatattat ctattgtttt acctcttaat
    16681 ttcttgcctg cctttactgc ctgcaaagca cctgtttcag tcaattcaac atctgtaata
    16741 ccgctgaatc tcctgcttat atttgattcg gattctccgt gacgaactaa atatatcata
    16801 tctgcctcat taagaaaagt acgattgtca taaagactat ctggcttatt tctgtgataa
    16861 atccgcatgt atcccctgtc attccgccta ttttttcttc tatcagtatg gatatgaaat
    16921 atgaaacagc aattgcagca aaacatgcag taaacattat agcccggtac catggtatca
    16981 ttgaaaaaac aaataatatc aataaattaa tcaataatac atttttatca atattttcta
    17041 taaatacagt tcccatacct tttattttct tagcatattt tttattccaa caagaaacta
    17101 ttgtgcttgc ccgtcctaca agaggcatca taaaaacagt ttcaatgctg caaaaagaaa
    17161 acagaacaaa ttgtgaaagt ataattaaaa tcaatgctat aacaccaaaa gctcctatgt
    17221 gggaatcact cattatttca aagattctat cccctgtcct tccggataat aagccgtctg
    17281 cagtatcaca agcaccatcc aaatgtatac cgccgcttat aagaatgtac ataacagtaa
    17341 ttaccagccc tcttataaag gatatttgat tgttaaaaat taaataaaca acagccaaaa
    17401 gaaaacctat taccactcct actaaagtga aaagattcaa tgcttttaca aagttttctt
    17461 cattatactc tattttttcc ccaacaggaa tccgagtaaa aaaggaaatg agaattagaa
    17521 atcctgaggc cattttatca tttctccttt atgtttaagt ttaattggta ttcctgaaaa
    17581 aataaaatac atatgctcgg cttggactcc aattttattg ttaatccgcc cgcttatatc
    17641 cctgaaataa tttcccatgt agtaagaagg tacaagaccc attcctgttt cattagagac
    17701 tattataagt tttttattgt tttccttgca tacattaatt aatgaaaaaa cctcttcggt
    17761 aattttgttt tccaatatat ttacatcttc aacactgcaa tcatcaaaat ccaatttgct
    17821 ttcaaccatg taatttccaa tcatcgttgt tatgcagtcc aaaagaacta cttcagactg
    17881 attgaattca gctttattaa taagtgtttt aaaatcagag tacatctcca atgtctgcca
    17941 gaaagccggt ctttgctccc tgtgcttttt aactctatct gccatagcct cgtctgttac
    18001 ggctgcagtt gctatataca atacgttttc cgaatattct tttgctttta attcagcaaa
    18061 ggtgctcttc ccgcttcttg caccgccgat aacaaatata agactcattc tatcaccatt
    18121 taatattttt aaccattaac atttaacagt atatcattta tttaataaaa aataaacaat
    18181 gaattttaaa attttaagga cgggtgttct cgcggtgcct ggcaccgggc tgcattaaca
    18241 aaggacggtt ccaaatagaa ccgtcccgaa attttattga cattgatatt gattataatt
    18301 aagatatatg taaagggcga aaggataaaa ttatgagaat aacaggtgtt gtggcggaat
    18361 ataatccatt tcattcagga catgaatatc agttaaggat ggcaagaagg ctatcaggct
    18421 gtgattgtat tgcagttgta atgagcggaa attatgtaca gcgcggagag cctgcaatta
    18481 tagataaatt taaacgggcg gaggctgcaa tttatggcgg tgctgatatt gttatagagc
    18541 ttcccatgcc tttttcatgt caaaatgctg aaatgtttgc tcatgcagca ataatggaat
    18601 taaaaaagct taatgttgat tcaataagct ttggctgtga aaccgacaat gtaagactat
    18661 tgaaaaaaat tgctttatta caattaacac ccggttttaa caattatatt aaggaagaaa
    18721 tgaaaaaagg attatcatat cccaatgcca tgtctaatgc acttaaattt atacttaatg
    18781 aagaagcaga agccgtatat tcacctaata atgtcttggc tgtcgaatat ataaaaagtg
    18841 ccgtgaaact taacttagaa cttcaatact atcctgtaaa acgtgtggga atggctcaca
    18901 atgacaccga aatttcaggg ctctttcaca gtgctaccgc aataagaaat gccataatgg
    18961 aaggctcttt tgataatctt aagcttacgg aaaaaagtat tgaattgata aaaaaattct
    19021 gcaaggagca caaaaaatat aatatattga acaattatag ggattatttg tattataaaa
    19081 taattacatg tgacgattta gaagaaattt atgaaataaa tgaaggatta aacaataaaa
    19141 taatatcaca ggtttacaag catgaaacta ttgatgaatt tataatgtct cttaagtcaa
    19201 agcgttatac ctattcaaag ttgagaagaa gccttctgaa tatcttactc ggcatcaaaa
    19261 aggttgacat aattaatatt atgtcaacca atgataaata tgttaaggtt cttgctttta
    19321 ataataccgg caggaaggca ataaaaaatg ccgtaaataa tggaaccata gtaataaaca
    19381 gatattccga ttataaaaag aataatttaa ctcctgaaaa acttatgagg tttaatataa
    19441 caaacaaaac atcaaacatt tattatcttc cgttggaaaa cagaaaaatg aacagcgaat
    19501 atttcgaaaa tgcagtctat gtagatgtat aattttaggg ggcccgttta tttactgaag
    19561 aattgtgatt gtcataataa ttatatacta agcatataaa gcactcttgg acaataaaat
    19621 aaaaaggggg tgtttttatg cttagaagaa attttattcc tgttttcata atccttatta
    19681 tttgcttatt cgttatgaaa ccgggcatag tatcggaagg tgttttcaac ggtcttaaaa
    19741 tatgggtcaa caacatgatt ccgtatttgc ttcccatgtc tattttatca aatattttgc
    19801 tgcaatataa ttttttatac agaatatctg agaaactatc ctttttatcc aataaaattt
    19861 ttaacagcaa atatgcattc atcccctatt ttatcagctt tgtggtaggc tatccttcag
    19921 gtgcgatgac agtaaatacc atggcttcgt ttaaaagaat agacagcagt gaagcaaatg
    19981 ctctgatggt tttcacaaac aactgcagct ttcaatttat ggcaggagca gtatcttttt
    20041 caatgctcgg cgatttaaaa ctagccaagt atattgccat tcctcatttg ttaggtgcat
    20101 tacttatagg gattatcata aaaaaaggca gtatatctac tgcttttaca aaggaaaaaa
    20161 aacataaata catttcattt tacgatgctt tcaattcttc aatttataaa tcgattacct
    20221 caattttatc catcgggggc gtaattgtta tattttctgt attttccaat ttttttgaca
    20281 acctcctaat atcagtttca aaatatctcc cattaagtat aaatctaagc gatataatac
    20341 attctgtttt aacaggaata ttagaaattt caaacggctg cagcgttaca gcttcctctg
    20401 tacttccctt ggacgtaaag cttataataa tcaattttct tatcagcttc tcaggaatat
    20461 cagtaatttt tcaaaccatt gccgttgcaa atgattttga ttttaaaatt agcaattatg
    20521 ttttttatag agctatacaa ggaatattat cggttataat atgtatcggg atgttaattc
    20581 tatcttgaaa agtattgtaa gtatatatat aatggtatta cttaagaaaa aggtgatata
    20641 atgataaacg ttactttaat cggtacaggc ggtatggtac cgcttcccgg aaggttttta
    20701 gcttcatgtc atattgacta tcaaggaaaa gcaattctta tagattgcgg agaaggtaca
    20761 caaatttcac tccataaggg aaaaataagc ttaaataaaa ttgatacgat attgataact
    20821 cattgtcatg cagaccatgt aacaggtctt cccggactgc ttttaaccat gggaaatcac
    20881 ggcagaacaa atcacataga tattattgct ccaagaggaa gtactaaaat attgaactcc
    20941 cttttaatgg tttgcggatt tcttcccttc gaggttagaa ttgcggagct tcacgacaca
    21001 aaacctcaga aattcagtaa aataggatta aatattacat cgattccttt aaaacatcat
    21061 ataaactgct taggctacag ctttgagctt attttaagac cacatttcca gcccgaaaaa
    21121 gcaaagaaac taaatattcc ggttaaacta tggagactcc ttcacggggg taatgaagtt
    21181 gttgtagatg atagaacaat tacaccggat atggttttgg gtagtcccag aaaacccatt
    21241 aaaatttctt atgtaacaga tacaagaccg gttaaactta tagatgaaat cattaaggat
    21301 tctgatttgt ttatttgtga aggaatgtac ggaaatgatg agatgtttga gggtgctgtt
    21361 gcaaaaatgc atatgatttt ttcggaagcg gctaatattt cattaaatgc taatgttaag
    21421 gaattatggc ttactcattt cagtccctca atgccaaatc ccgaagagta caaagaattt
    21481 gctgaaaata tattttcaaa cactgttatc ggtaaagatt taatgagcaa gactttaagc
    21541 tcaccataat aaaaggagcg gaaacccgct ccttttaata ttgcaataaa aaatg
//
LOCUS       DAOPUE010000027        21323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2458_length_21323_cov_10.538179, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000027 DAOPUE010000000
VERSION     DAOPUE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 21323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21323
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_21323_cov_10.538179"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..1269)
                     /locus_tag="PKH42_04000"
     CDS             complement(382..1269)
                     /locus_tag="PKH42_04000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOA19628.1"
                     /translation="MKCIVYYRKEFETSMNKKPIVAIMYDFDKTLSPKDMQEYGFISE
                     LGMKSEDFWEKSRLNMVNHNMDQILAYMLTMLQEAEGRMLLTRNIFNEMGRNVKLFEG
                     LETWFDRINLYGEKLGLEIEHYIISSGLKEIIQGTEIAHNFKEIYAAEYCYNENNIPI
                     WPAMAVNYTSKTQFLFRINKGVLDVTEHKGLNESTPDNKKRIPFCNMIYIGDGLTDVP
                     CMKLVKINGGHSIAVYHDDRCIADSLISQGRVDFVVSADYRENSEMEKTVQTILNQIA
                     ALNKVLKLHTKHMKNLITD"
     gene            complement(1398..1922)
                     /locus_tag="PKH42_04005"
     CDS             complement(1398..1922)
                     /locus_tag="PKH42_04005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOA19629.1"
                     /translation="MESENIIIRKSTFDDCKIFTEWEQQDYVKEFLTINKERTYEEVV
                     REFILREQDPTKEQYTIILKNTGPIGRVYLSNISREFDFIDITRIYIGEKEHLGKGYG
                     RECMELLLDYCFNILKMERVTLDHYTGNKASNLYLKLGFKYEGIMRHAAKKDGKYYDL
                     HLMSMLREEYKQSI"
     gene            complement(1967..2485)
                     /locus_tag="PKH42_04010"
     CDS             complement(1967..2485)
                     /locus_tag="PKH42_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOA19630.1"
                     /translation="MLLILALLAHEVSHVFSAILLNIEFSKVKITLFGINLNAHLDNV
                     SHAKKIILFFAGPLCNLCFYFGFRNTEYFCFANMNLLLAYINLFPIVPLDGGNICKTI
                     LEIFLDTRTVSGYITMTNAFFILFFIIIIYIFKDYLFFLLVVMGLKGIVEENSYLLEK
                     SIIAVYNKINTK"
     gene            complement(2499..3122)
                     /locus_tag="PKH42_04015"
     CDS             complement(2499..3122)
                     /locus_tag="PKH42_04015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOA19631.1"
                     /translation="MNKLTKKRLNIKRKEQVNKKLVLQVIFSIVLVAAVIVTKNINSN
                     LSEKIIEAADEKMSASIELLEVRDTIAKGFTTAINKLPFVGKEEASPVIGTLYRKYGL
                     NKTDDVSYYNHGIDIKSETQSVKALTDGKVMSVGKNEKHSGYIVIQDDDRTLIYGKIN
                     ETFVKEGDNISKGDVIGALDEENMILHIEVWEDGASINPSSLFDLTK"
     gene            complement(3265..3948)
                     /locus_tag="PKH42_04020"
     CDS             complement(3265..3948)
                     /locus_tag="PKH42_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19632.1"
                     /translation="MNVILTGSQKNNIGKTIISIKLAIELANSGKNVLMMDLSSGKIK
                     MSEYLNVNEDIIYDIVDVVNKTCTLEQGIIEINENLSLLPCPRIAGKINEIKKESFIN
                     LLQSVEVYDYVIIDADKLTSVFIDFSIINNAITINNNDFSCIKEINTDKTISSNAGNF
                     IVAINKYNKKKAKKGIMMKSNDIEKLTETTISALIEENIKYSDLNHEKFLDDNFLKTE
                     IKNIVKNLK"
     gene            complement(3984..7316)
                     /locus_tag="PKH42_04025"
     CDS             complement(3984..7316)
                     /locus_tag="PKH42_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOA19633.1"
                     /translation="MIKFFKNRFNILYLIIVLLLIILGFRMAVLTIVEGQEYRQIADI
                     KKIKDIPVKAPRGKIFDRNGIILADNVSSFTVQMYTDEIKPEDFNEIAYKLSKILDEN
                     MERPIDDFPIELDTFDYINESVEIIDSDLNLEEEIEVNYYRTAEEKVIEKVKENIHEW
                     LNFSISIYGENFSPKQRVHAAIINDNVPINLEGDKFEYEDEMLIQTDSDLTEAENETT
                     EQKTPIQIWLSEHNLKENISAEEAVAQLLVNNNKYLSSLFSNSKIRRLSYEFIIQKGV
                     ENIKLNEFSFIQDEEYENIKRDLNALNEEITMDTSAKDDFVILTMKNSIDNLLQTVYQ
                     GNEKIMPGAILIERLKEIYPDLPVKFVENGELGVFEYTDTETRNKYFNQQHMEYSTTA
                     YDFIKEIAFKNYDVLYNLVTDDNIKYYSQIELLKYVNPSISINEWEYTPLRNKKNWIN
                     NNVELDEETDDYSAKKVFEMLRESLKIPEEINDYDMRNMMVIRERYNIKSYLSYHPVD
                     ICYSISQKTVAMISERHHELNGINIEIEPLRYYPQYESASHIIGYLGKIAQDYELHEY
                     VVEKGYSTDDIIGKTGLEEKFEDYLRGEKGKKTVEVNNVGRTIRSVSSEAPVPGDDLY
                     LTLDLRLQKKAEESLKKGLEKIREGGVFESEWGDFQFRDKYENAYSGALVALDVKTNE
                     ILALANYPAYDLNLFSTGITTENWNSLLNDSKNPLAPRPLYNIAMLTAIQPGSTFKMI
                     TSLAALEKGVNPNTTVYCAGTMKVGDRDFRCWIYNMFGGRHGSQTMYQAIMNSCNFYF
                     YTTVLGENLATHQKHTVKVDAEDIIEMASKFGLDSKTGIEIDIPQEASGGVPSIEGKK
                     SGIRVYLRLFLEANIERYLNDGVVIDASMKNEIIEEIASWIDRDELMTRAEVYEGLLA
                     LNLNPDKTNDNYVPLVDIIKYSYLNQATWTVGDNLNISIGQGNNAYTALQMANYVSSI
                     ASGLRRNVSVVKEIKTYDGKKTDYIPLRESEKIELSDDSYLDIVKHGMKLVSYDDNAK
                     PFATFPVEVGSKTGTAQNQGINPETGQPYDDFAWYVAFAPYDDPQIAVACVLFEGGSG
                     RYPIPIVREVIGEYLKINGLIE"
     gene            complement(7323..7817)
                     /gene="mreD"
                     /locus_tag="PKH42_04030"
     CDS             complement(7323..7817)
                     /gene="mreD"
                     /locus_tag="PKH42_04030"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOA19634.1"
                     /translation="MKKLILMSSLIVIGFVFQTSFIGFFNYFKILPNIPLILLVIFAM
                     LSDGVEGGVLGILTGILHDAMIYDVFGIYTLIYFIIGAVIGAFSDEMLRENKLVYSAV
                     TGISTVVMHLLLFLILFFLRFRVHLAKSILPNILFETALNAVLVIFVLKFVIYIFDRF
                     NVKA"
     gene            complement(7837..8670)
                     /gene="mreC"
                     /locus_tag="PKH42_04035"
     CDS             complement(7837..8670)
                     /gene="mreC"
                     /locus_tag="PKH42_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOA19635.1"
                     /translation="MNFLKEHFKKILFFLTIFILIIMIGLSSIGRFEGADLGFIGNLI
                     AGFQKLTYKTGNTLTNSFYSVQDIVKMREENIMLTESIYKLREQVRILENIINKSEAL
                     ETEYEMRKNLTYDYVVGQVIAIDDSNWFSRFTIDKGENDGIRQNDIVIQAVKTDDGIV
                     QIGLLGIVTQTSSDWAKVITLLDEGCKVSFKNIENDESGIVQGSIDGNVTGYFFNSKA
                     GAEKGDNLVTSGIGEIYIPDIYIGDVKDVVKTTDASTLRIIVEPAVDFTKISRVYVLT
                     K"
     gene            complement(8719..9870)
                     /locus_tag="PKH42_04040"
     CDS             complement(8719..9870)
                     /locus_tag="PKH42_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOA19636.1"
                     /translation="MGLFDFFTQKIGIDLGTSNTLVYVNNKGITLNEPSVVAINTTSY
                     EVCAVGFDAKAMLERTPKTIRAIRPLQNGVIADFEITRAMIKYFITKALNKKRFIKSN
                     IVISVPVGVTSIERRAVEEVAYDAGARKVKLVEEPMAAAIGAGLNVDQPIGNMIVDIG
                     GGTTEIAVISLGGIVESDSLRIAGDDIDINIKEYIKNKYKMDIGIVTAENIKKVLGNA
                     SKEYYKRIIDSDERIDSKEANEDNEDFEINEDNKDKKDLKDKKDKKEKKDEKENKKTD
                     YVQQMEIRGRDIVSGLPLKITITSDEVREAILETVNSIVNAIKRVLERTPPELSSDIY
                     TNGIYLSGGGALLKGLDIYLEKETGIKVFIADNPLQSVALGAGKISEEL"
     gene            complement(9888..10577)
                     /gene="radC"
                     /locus_tag="PKH42_04045"
     CDS             complement(9888..10577)
                     /gene="radC"
                     /locus_tag="PKH42_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOA19637.1"
                     /translation="MDNTNYTIKSLPADDRPQEKLLKYGAGALSNSELIAVILRTGTR
                     EENVVMLSQRILKEGGKGLRNIAEGSLEKFKSYKGISDAKAAKLMALAEISKRMSTLK
                     IEKLKISSPDDAAVMMMEEMRYYQKEYFKIMLLDTKNNVKKVSEISVGSLNSSIVHPR
                     EVFYEAVVNLASSIILVHNHPSGDCEPSHEDIVLTNRLDESGKILGIRVLDHIIIGDG
                     VYFSFKEEGLI"
     gene            complement(10604..11179)
                     /locus_tag="PKH42_04050"
     CDS             complement(10604..11179)
                     /locus_tag="PKH42_04050"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOA19638.1"
                     /translation="MKKIVLASNSPRRIEMMKKYVDIITYIPDIVEKVNSFDYPQTTV
                     MKVAFEKALNVYLKYKENALIIAADTIVYLDEIMGKPKDEAEAYKMLSLLSGKTHYVF
                     TGICIIDTESSKKIVDYEKTQVVFNELTEGLIKRYLKTYEYLDKAGAYGIQGYGELLV
                     KEIHGCYNNVKGLPIAKLNNLLSKHFSYDLL"
     gene            complement(11188..11430)
                     /locus_tag="PKH42_04055"
     CDS             complement(11188..11430)
                     /locus_tag="PKH42_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOA19639.1"
                     /translation="MRKGNSFYLILLILTSVIIGTILGKAFSNMLPILNYGEGIGFGP
                     ATLDLNIFTITFGFSARLTVAGIIGLIAGIIIFRKL"
     gene            complement(11430..11951)
                     /locus_tag="PKH42_04060"
     CDS             complement(11430..11951)
                     /locus_tag="PKH42_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOA19640.1"
                     /translation="MNKIHRYIFLSLLTAAALILSIIEGMMPLPYIAPGAKLGLSNIV
                     ILSTVVVFGFKDALLVVIIRSILLMLVATNPITFIYSIASGIVSTIVMSLACKYLSNI
                     FSLIGISVLGAMAHNTTQVTVAAILFTTVNLYYYLPILSLVSIFTGCFVGYTAIFVTN
                     NLETTVKTWRRGV"
     gene            complement(11964..12353)
                     /locus_tag="PKH42_04065"
     CDS             complement(11964..12353)
                     /locus_tag="PKH42_04065"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOA19641.1"
                     /translation="MNKKINKYDIGLIVSIIIISLFFILYGGRDAVISNSKTAYIYSN
                     NKLVGEYVLTDDYKDVVNIESETGYNIMHIENGQIWIHEASCPDKICIYQGKISKNGE
                     MIVCIPNRMFIKIVDENDESEIDFIAD"
     gene            complement(12517..13149)
                     /locus_tag="PKH42_04070"
     CDS             complement(12517..13149)
                     /locus_tag="PKH42_04070"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HOA19642.1"
                     /translation="MYINLDVLNITNGNTHIIDSVFGRMNIEKIEAGKLKLLTGRIIT
                     TDPVLLYDDECFSEHVKPGLYTVNIYVGKCGKRKKQTVAAELRINENVPVKWEMALLE
                     GESAKGFSSDEFMGYEVENGLGCFMDEKVMEMLDIMSEEELNDYETKVKDAVRQSDSS
                     CADIIMDEEKGLNIIVFASGWNEGTFPTYYGFDKNNKLSRLVTDFMVIEK"
     gene            complement(13159..13620)
                     /gene="nrdR"
                     /locus_tag="PKH42_04075"
     CDS             complement(13159..13620)
                     /gene="nrdR"
                     /locus_tag="PKH42_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOA19643.1"
                     /translation="MRCPKCNYTESKVIDTRPTDDGFKIRRRRECIECGHRFTTYEKI
                     EETQIVVIKRDGTRQGYNRDKIINGLIRACDKRSVSLEQIEKIADKVEKQLYNTFQNE
                     VSTEIIGELVMNELQNVDDVAYVRFASVYRKFKDIDTFMDELTKILNERKK"
     gene            complement(13650..14033)
                     /locus_tag="PKH42_04080"
     CDS             complement(13650..14033)
                     /locus_tag="PKH42_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOA19644.1"
                     /translation="MELNLSVGLKYKEEKTVTINDTAKVFGSGAAEVFATPMMIGLME
                     AASMNAVKNFLPRGCSTVGTLVNVKHMSATRVGKKVWAEAELIEIDRRRLVFNVTAYD
                     EDKKIGEGTHERFVIDDEKFMSNLK"
     gene            complement(14178..15527)
                     /locus_tag="PKH42_04085"
     CDS             complement(14178..15527)
                     /locus_tag="PKH42_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19645.1"
                     /translation="MQARGNISNLFVRLKFSAMMALDGITSNLLRTFLTVLGISIGVA
                     AVISLMGIGEGARRSIVAQFESLGENVIVIKSNADNVKFEIEEAEILPERVTGIEYAT
                     PVIYTEDTPVRWRRDESEMNIVGVNESYPQIKDHPVISGNFFSSLHVKQRSPVVVLGY
                     NVAKSLLNGRSPVGYTMKIDGINYRIIGVLGEKGEGKGDGIDDKIVIPYTSAMKIAKT
                     KDILEIWAKSNSAEDAELAMVQLGRIYKRKVGLGSNIKPSTGEGEQPPGEGGGMDMPI
                     EEPMPEPVPEEPEEPGEKENVFEKGEEPLTITSLNNLVKEADQANRIMTVLLGGIAAV
                     SLLVGGIGIMNIMLVAVTERTSEIGVRRALGAKKEDLLIQFLLEAVYVSIIGCVAGIV
                     LGAWAISIVAKYGLSAVVSLDSIRIAVFVALTSGLLFGVYPAINASNLPPVEALKRQ"
     gene            complement(15540..17282)
                     /locus_tag="PKH42_04090"
     CDS             complement(15540..17282)
                     /locus_tag="PKH42_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA19646.1"
                     /translation="MYKKIISIAIVLGLVFFGGYLTAKWLIPKDTEASAGPRYSTKAV
                     ERGDIKQGVNLSGQLSGNWGGSITAPRPEGITDSNGMSVSVTYTVEEVFVEPNQMIKK
                     GDNLVRLSAVNLGDILTELTDSIQKKQEDIQAKISNLEKILNKDVTDISQVNAYDGIV
                     FRAPIRGRITELSVEEGEKVENFNIATIVDDSMVKISFKVNTYEFESLKEGQKVLLQY
                     RKTTEHGSQLAFDGFYPGTIKKLNKNKVPNGMTYVHTGTIEAENPGLVQPGMTVSIYT
                     ENNGIPVTALSYTGEVNSFVNEKKVTYSYLHGGENSNVIATEVYVSTNEFVEEGDLLV
                     RIAGSDVTQLIQNDIDEITNAYKEIENIYRRIDKISELTSKLMVTAPTDGMVAYVNYR
                     VGDQITANEQSDQWSLMLMDVYNTDEMYIYTNASDLDVLYIAQDAPVIVTVDALPGET
                     FDGKVMRLDSYTDYRTGETVYNVQIRVEGREGLRPGMNTNCFVDSGESLDTLLIPIEA
                     VFEENGKQKVEILKEDNQVEVVEIETGLMNDMYVEVISGLEEGQLVVTGSTKDLMPSQ
                     SVPESDIILPGTNK"
     gene            complement(17275..17973)
                     /locus_tag="PKH42_04095"
     CDS             complement(17275..17973)
                     /locus_tag="PKH42_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19647.1"
                     /translation="MDIIRMQGVSKIYKSGQIQVEALKNISFNIKEKEFVSIMGPSGS
                     GKSTLLNLVGCLDHPTSGTYELAGNQVDKLSDSKMSDVRNQFIGFVFQSFHLLPRMTA
                     IKNVEIPLIYRGMSDKKRRELAENALELVGLKDRMTHLPAQLSGGQQQRVAIARAIVG
                     NPSIILADEPTGALDSKSGELIMELFVELNEKNSMTVVQVTHEEFIAEYGSRIIRLVD
                     GQIVSDDVVEVKNV"
     gene            complement(18100..19500)
                     /locus_tag="PKH42_04100"
     CDS             complement(18100..19500)
                     /locus_tag="PKH42_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19648.1"
                     /translation="MNNNYKIIRTIYENAFQRILECKDDDTGAIFYSNIITSKKVINL
                     ININDLKKIKSNILECYNTEDRIYIFTKSLETDCRKLTDLANSNLTMKQQFSLAEKSI
                     NLASNIYNMTDVVQQKILDIDRLFIDDNNDLIVDCNLIFEQEYDIADNETFKRLGNII
                     HMIFSGIEIIDYNISDSIPPDILKIIVRCLTREYVFPNDALVELKRSPIFNMLFGEEI
                     INNTVDIEQPEEPSLVIHNEESDDYLNIYLEDNQPAKKKALINKDIKRAAVSLLTVVM
                     VLLLGNFIIKKFNKNDEAEAINNVKPPLQEAPSETPVEEEPVYESTDIHFSEELLESI
                     GYEGAKAQKDTDIYVEGSSSLTVANSGQDKVKALFAAVDFKDDNFNYMLMKQIGVSGK
                     IKSQYDTQARVVFEAYKDGVIKSNFHSLVNIYDDIWSQFTVPINVTEADVLYIYLEYE
                     GENKVWIDSLFIDVIK"
     gene            complement(19596..19877)
                     /locus_tag="PKH42_04105"
     CDS             complement(19596..19877)
                     /locus_tag="PKH42_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOA19649.1"
                     /translation="MGRILYIIKFLFDKNIPIREKWWVIIPLIYILSPADLIPFPILG
                     LGIVDDLVMLGFLLTIVYDKTKKYYFDKRHEDKDIIENVEYEIDEDEEK"
     gene            complement(19887..20255)
                     /locus_tag="PKH42_04110"
     CDS             complement(19887..20255)
                     /locus_tag="PKH42_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA19650.1"
                     /translation="MPNCVLVCVTRQKTSEKLIKKGVELIKGEAGSCLNVIHVVNEND
                     KFLYGLSDGDALEYLFEITKDVGAANLVVKRSKDVIKTIVEYAEEINCTHIVLGHSNN
                     PSNNFIAKLQRKLPNVEFII"
     gene            complement(20273..20367)
                     /locus_tag="PKH42_04115"
     tRNA            complement(20273..20367)
                     /locus_tag="PKH42_04115"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20331..20333),aa:Sec,seq:tca)
     gene            complement(20481..21167)
                     /locus_tag="PKH42_04120"
     CDS             complement(20481..21167)
                     /locus_tag="PKH42_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA19651.1"
                     /translation="MKVLIVDDELLTIKNLKYSFEQDNYEVESTGDSNEALKKITTTD
                     YDIIILDLVLPQTNGLEVCQKIREISNVPILILSANNDDMSKILGLEYGADDYITKPF
                     NILELKARMKAILRRTRGTSQKANEQTLVVDDFIINALGRKVSLHKQEINLTAKEFDL
                     LLLLITNPGKVFSREELLEIIWGYEYYGDLRTVDVHVRRLREKIETKNKAYEYILTKW
                     GVGYYFRNRN"
BASE COUNT         6213 a         4335 c         2860 g         7915 t
ORIGIN      
        1 gtcaattaag ccatccaaaa aatacctgaa acagaaaaat tgatttttaa tttatttatg
       61 cattcttata attagcgttc atttattcac atataatata cggggggatt atattgattt
      121 taaaaatatg ggaatttcta aaaaaacata tttttattat aatttttata ttttgtattt
      181 tgactatttt attttcactt ttacatacat gacgaggacg ccttccccat gcctataaaa
      241 gggcaagccg tgaatacatt cacggcttga ggcaaaaaat ttaagaggca tttataaatt
      301 ttcatttaca aatgtattta tattatacta cttttttaaa aaatttcaaa tagttttttt
      361 attcttcatt tatgcattaa ctcagtccgt tataaggttc ttcatatgct ttgtatgcag
      421 ttttagcacc ttatttaaag ctgctatttg attaagaatc gtttgaactg ttttttccat
      481 ttcgctgttt tctctatagt cggcactaac gacaaaatca accctgcctt gtgaaatcag
      541 gctgtctgca atacacctgt catcatgata gactgcaatg gaatgcccgc cgttaatttt
      601 gactaacttc atgcacggca catcagtcaa accgtcacca atataaatca tattacagaa
      661 aggtattctt ttcttattat ccggagtgga ttcgtttaat cctttatgct ctgtaacatc
      721 caacacaccc ttatttattc tgaacaaaaa ttgagttttg ctggtataat ttacagccat
      781 tgccggccat ataggaatat tattttcatt atagcagtat tcggcagcat aaatttcttt
      841 gaaattatga gcaatctcag ttccctggat tatttccttc aatcctgaag atattatata
      901 atgctctatt tccaatccca acttttctcc atataagtta attctgtcaa accaagtttc
      961 tagcccttca aataatttca cattcctgcc catttcatta aatatatttc gggtcaaaag
     1021 cattctgcct tctgcttcct gcaacatggt taacatatat gccagtatct ggtccatgtt
     1081 gtgattaacc atatttaacc tgcttttctc ccaaaaatcc tcggatttca tccctaactc
     1141 agagatgaat ccatattcct gcatatcctt cggtgaaagt gttttatcaa aatcatacat
     1201 tattgcaact atcggttttt tattcatgct tgtctcaaat tcctttctat aataaacaat
     1261 gcacttcatt gcaacatctt ttataccttg tagttatata ttataacttc ttcgcctttt
     1321 ctattatttg catccgaatt aatcattctt ttcacgctta ctacatcaat acaaattttt
     1381 tgaattttca attattttta aatagattgt ttatattctt ccctcaacat tgacattaag
     1441 tgcaaatcat agtatttacc gtcttttttt gcagcatgtc tcattattcc ttcatattta
     1501 aaacctaatt tcaaatacag attactagct ttatttcctg tataatggtc taatgttacc
     1561 ctttccattt ttaaaatatt aaagcaatag tctaagagca attccatgca ctctcttccg
     1621 taaccctttc caagatgctc cttttctccg atatatatcc gtgtaatatc aatgaagtca
     1681 aattctcttg atatattaga aagatagact cttccgatgg gtcctgtgtt tttcagaata
     1741 atcgtatatt gctcctttgt agggtcctgc tctctcaaga taaactctct aacaacttcc
     1801 tcgtatgtac gctctttgtt tattgtcaaa aattccttaa cataatcttg ttgctcccat
     1861 tcggtaaata ttttgcaatc atcaaaagta ctttttctaa ttattatatt ttcagattcc
     1921 atttatccct ccgactattt ggatttaaga gttgtttagg gttatttcat tttgtattta
     1981 ttttattata tactgctata atggattttt caagcaagta actgttttcc tccactatcc
     2041 cctttagtcc cataacaacc agcaaaaaaa acaaatagtc tttgaatatg tatattataa
     2101 ttataaagaa aagtataaaa aaagcatttg tcatagttat gtaaccggat accgtccttg
     2161 tatccaaaaa aatttctaaa atagttttgc aaatatttcc tccgtctaaa ggcacaatcg
     2221 gaaataaatt tatgtatgcc aataacaaat tcatatttgc aaaacaaaaa tattctgtat
     2281 ttctaaatcc aaaataaaaa cacagattac ataaaggtcc tgcaaaaaac aaaataattt
     2341 tttttgcatg tgaaacattg tctaaatgtg catttagatt gattccgaac agtgtaattt
     2401 taactttaga aaattcaata ttcagtaata ttgcagaaaa tacatgggaa acctcgtgtg
     2461 ctaagagagc taagattaaa agcatttccc aaaggtgttt attttgtcaa atcaaataat
     2521 gatgaaggat ttattgaagc accgtcctcc catacttcaa tgtgaagaat catattttct
     2581 tcgtcaaggg ctcctataac atctcccttg gatatattat ccccctcttt aacgaaagtt
     2641 tcatttattt tcccgtaaat caatgttcta tcatcatctt gtattacaat atagcccgaa
     2701 tgtttttcat ttttgcctac cgacattact tttccgtccg taagagcctt aaccgattgt
     2761 gtttcggatt taatgtctat tccatgattg taatacgata catcgtctgt tttgttcagt
     2821 ccgtattttc gataaagtgt ccctattacg ggtgaagctt cttctttacc tacaaaaggt
     2881 aatttgttta ttgcagttgt aaaacccttt gcaatagtat ctcttacttc caataattca
     2941 atgcttgcac tcattttttc atctgctgct tctataattt tttctgaaag attcgagttt
     3001 atattctttg ttacaataac ggctgctacc aaaactatgc tgaaaattac ctgtaaaact
     3061 aatttcttat taacttgttc ctttctttta atattgagcc ttttttttgt tagcttattc
     3121 atttcaccac cccttccaat acattttatt aatgtgttac aagctttatt acaaaataaa
     3181 aaaacttttg tccctgtatt accagtgaca aaagttaata tttcataatc cttcactatt
     3241 cctataaaaa cagaggaaag caggttattt taaattcttt actatgtttt tgatttctgt
     3301 ttttaaaaaa ttatcatcaa ggaacttttc atgattcaaa tccgaatact ttatattttc
     3361 ttcaattaaa gctgaaattg ttgtttcagt taacttttca atatcattgc tcttcatcat
     3421 aattcctttt ttagctttct ttttgttgta cttgtttatt gcaacaataa agttaccggc
     3481 attagaagaa attgttttat ccgtatttat ttcctttata catgagaaat cattgttatt
     3541 gattgttatt gcattattaa ttattgaaaa atctataaat acggatgtta atttgtctgc
     3601 gtctattata acataatcat agacttctac actttggagt aagtttataa atgattcttt
     3661 ttttatttca tttattttgc cggctattct gggacatggc aacagcgata aattttcgtt
     3721 aatttctata ataccctgtt ccaaggtgca agttttattt acaacatcaa ctatgtcata
     3781 aataatatct tcattgacat tcaagtattc tgacattttt atctttcccg aggacaaatc
     3841 catcattaga acatttttgc ctgagtttgc aagctcaatt gctaacttta tacttattat
     3901 tgtcttgcct atattgtttt tttgtgaacc tgttaatata acattcattt tttatccccc
     3961 tataacgact attctgcatt ttgtcactca atcaaaccgt ttatctttaa atactctcct
     4021 ataacttctc tcactatcgg aataggataa cgtcccgagc ctccttcaaa aagtacacat
     4081 gctacggcta tttgcgggtc atcgtaaggt gcaaatgcaa cataccacgc aaaatcatca
     4141 tagggctgtc ctgtttcagg attgattcct tgattttgag ctgtaccggt cttgctgcct
     4201 acctcaacgg ggaaggttgc aaatggtttt gcattgtcat cataagaaac aagcttcatc
     4261 ccatgtttta caatatctaa atagctgtca tcggacaatt cgattttttc ggattctcta
     4321 agcgggatat aatctgtttt ctttccgtca taagttttta tttcttttac tacgctgaca
     4381 tttcttctta agccgcttgc tatggaagaa acgtaatttg ccatctgcaa tgctgtatag
     4441 gcattatttc cctgacctat gctaatattt aaattatctc ccactgtcca tgttgcctgg
     4501 tttaaatatg aatattttat tatatcgact aagggtacat aattatcgtt tgttttatcg
     4561 gggtttaaat ttaatgctaa tagcccttca tatacttcgg ctcttgtcat cagctcatcc
     4621 ctgtctatcc agcttgcaat ttcttctata atttcatttt tcatcgatgc atctataaca
     4681 accccgtcat taagatatct ttcgatgttt gcttctagaa ataatcttag gtaaacacga
     4741 attccggatt ttttaccttc tatgctcgga actccgcctg aagcttcctg gggtatgtca
     4801 atctctattc ctgtttttga atctaatcca aacttgcttg ccatttctat tatatcctct
     4861 gcatcaacct tcacggtatg cttttggtga gttgctaaat tttcacctaa gactgttgta
     4921 taaaagtaaa agttacagga attcattatt gcctgataca ttgtttgaga gccgtgcctt
     4981 cctccgaaca tgttataaat ccaacatctg aaatctctat cccctacctt catggttcct
     5041 gcacaataca ccgtagtatt aggatttact cccttttcca aggcagccaa ggatgtaatc
     5101 attttaaagg tggagcccgg ctgtattgcc gttaacattg caatattgta aagtggtctt
     5161 ggagccaaag ggttttttga atcatttaac aggctgttcc agttctctgt ggtaattccc
     5221 gttgaaaaca aattcaagtc atatgccggg taatttgcca aagccaggat ttcattggtt
     5281 ttaacatcaa gtgcaaccaa tgctcccgaa taggcatttt catatttgtc tctaaattga
     5341 aaatctcccc attcgctttc aaatactccg ccttcacgta ttttttcaag acctttcttc
     5401 aaagattctt ctgctttttt ctgcagccta agatccaagg ttaaatacag atcatcgccg
     5461 ggaacaggcg cttcgcttga tactgagcgg attgttcttc ccacattgtt tacttcaaca
     5521 gtttttttac ccttttctcc ccttaaataa tcctcaaatt tttcttcaag ccccgtcttg
     5581 cctattatgt catctgtact atagcccttt tcaacgacat attcgtgcaa ttcataatcc
     5641 tgagcaattt tccccaggta gcctataata tgagaggcgc tttcatactg gggataatac
     5701 cttagcggtt caatttctat atttattccg tttaattcat gatgcctttc agaaatcatt
     5761 gcaacggttt tttgtgaaat actatagcaa atatccacgg ggtggtatga aagatagctt
     5821 tttatgttat acctttccct gattaccatc atatttcgca tatcgtaatc gttgatttct
     5881 tcaggtattt ttaaggactc tctaagcatt tcaaatactt tttttgctga gtaatcatca
     5941 gtttcttcat ctagttctac attgttatta atccagtttt tcttgtttcg caaaggtgtg
     6001 tattcccatt cgtttattga tattgaagga tttacatatt ttaaaagctc tatttgggaa
     6061 taatatttta tgttatcatc cgtaaccaaa ttgtataaga catcataatt tttaaaagct
     6121 atttctttta taaaatcata ggctgtagtg ctgtattcca tatgctgttg attaaaatac
     6181 ttgttccggg tttctgtatc tgtatattca aatactccaa gttcaccgtt ttctacaaat
     6241 ttaaccggca agtcgggata aatttctttc agtctttcta taagaattgc tccgggcata
     6301 attttttcat ttccctgata tactgtctga agcaaattat ctatagagtt tttcatggtc
     6361 aaaataacaa aatcatcttt agccgatgtg tccatagtaa tttcttcatt caatgcattt
     6421 aaatctcttt tgatgttttc atattcttcg tcctgtataa atgaaaactc gttaagctta
     6481 atattttcta ctcccttttg aataataaat tcataggaaa gtctccttat ttttgaattg
     6541 gaaaaaagtg aagataaata tttattgttg ttgaccaaaa gttgtgccac tgcttcttca
     6601 gcacttatat tttcttttaa attatgttct gaaagccaaa tttgtatggg agttttttgc
     6661 tcagttgttt cattttctgc ttcagttaaa tccgaatcag tttgtataag catttcatcc
     6721 tcatattcaa atttatcgcc ctctaagttt attggtacat tatcgtttat aatggctgca
     6781 tgaacccttt gtttgggaga aaaattctca ccgtatatag aaatgctgaa gttcagccat
     6841 tcatgaatat tttccttgac tttttctatt actttttctt cagcggttcg gtaatagttt
     6901 acttcaattt cttcttcaag atttaaatcg ctgtcaatta tttcaacact ttcatttata
     6961 taatcaaatg tatcaagctc aataggaaaa tcatctattg gtctttccat attttcatct
     7021 aatatttttg acagtttata tgctatctca ttaaaatctt caggttttat ttcatccgtg
     7081 tacatttgca cggtaaagct ggacacattg tcggcaagaa ttatgccgtt tctgtcaaaa
     7141 attttccccc tgggtgcttt aacggggata tccttaattt tctttatatc tgcaatttgt
     7201 ctgtattcct gtccttcaac aatggtgagt acagccattc taaaacctaa aatgataagc
     7261 aacaaaacta ttatcaagta taatatatta aagcgatttt taaaaaactt tatcatttca
     7321 cctcatgcct ttacattaaa tctatcaaaa atatatataa caaattttaa cacaaatata
     7381 acaagcacag catttaatgc tgtttcaaaa agtatatttg gcaatatact ttttgctaaa
     7441 tgtactctga atcttaaaaa gaataaaata agaaataata ataaatgcat cactacagtt
     7501 gaaataccgg ttacggcact gtaaacaagc ttattttctc tgagcatttc atcgctgaag
     7561 gctcctatta ctgcgcctat aataaaataa atcaaagtat atattccaaa gacatcataa
     7621 atcatggcat catgcaaaat ccctgtcaga atccccagaa ctcctccctc taccccatcc
     7681 gataacatgg caaatatcac caataaaatc agcggaatat ttggaagaat tttaaaatag
     7741 ttaaaaaagc caataaatga tgtttggaat acaaaaccta taactattaa ggaagacata
     7801 agaataagct ttttcattat taagcctttc taatcattat tttgtaagta catatactct
     7861 gcttatcttt gtaaaatcaa cagcaggctc cacaataatt cttagggtag aagcatctgt
     7921 tgttttaaca acatctttta catcacctat ataaatatca ggaatataaa tttctcctat
     7981 tcctgatgta acgagattat cacctttttc agcaccggct ttgctgttga aaaagtatcc
     8041 tgttacattg ccgtcaatac ttccctgtac aattccgctc tcatcgtttt caatattttt
     8101 gaagcttacc ttacagccct cgtctaaaag tgtaataacc ttagcccaat cggatgaagt
     8161 ttgagtaaca atgcctaaaa gacctatctg tactatgccg tcatccgttt tcactgcttg
     8221 tataactatg tcattttgtc gtataccgtc attttctccc ttgtcgattg taaatctgct
     8281 gaaccaatta gaatcatcaa tagcgattac ttgtccaaca acatagtcat aagtaaggtt
     8341 cttcctcatt tcgtattccg tttccaaagc ttcggactta ttgataatat tctccaatat
     8401 ccttacttgc tccctgagtt tataaatact ttcagtgagc attatatttt cttcacgcat
     8461 tttcacgata tcctgtacgg aatagaatga atttgtaagt gtattgcctg tcttataggt
     8521 gagcttttga aagcctgcta tcaagttgcc gataaagcct aaatctgctc cttcaaatcg
     8581 tccgattgat gacagtccta tcattattat caatatgaaa attgtaagaa aaaataatat
     8641 tttcttgaaa tgctctttca aaaaattcat ttgctcaccg ctttcaaaca tcagaagaaa
     8701 ttgccacgca atttctcgtt ataattcttc actgattttg cctgcaccca aagcaacact
     8761 ttgcaatgga ttatctgcaa taaatacttt tattccggtt tccttttcaa gatatatatc
     8821 caagcctttt aacaacgctc ccccaccgct taagtatatt ccattagtat aaatatctga
     8881 agataattcg ggaggtgttc tttcaagaac cctttttatg gcattaacaa ttgaatttac
     8941 ggtttcaaga atagcttctc tcacctcatc gcttgttatt gttattttta acggaagtcc
     9001 cgacacaatg tcccttcccc ttatttccat ttgttgaaca taatcggttt ttttattttc
     9061 tttttcatct tttttttctt ttttgtcttt cttgtctttt aaatcttttt tatctttatt
     9121 atcttcattg atttcaaaat cttcattgtc ttcatttgct tctttgctgt caattctttc
     9181 atctgaatct atgattcttt tataatattc cttgcttgca tttcctaata cttttttgat
     9241 attttcagca gtaacaatac ctatatccat tttatactta tttttgatat attccttaat
     9301 attaatatcg atatcatctc ctgcaatcct tagagaatcg ctttcaacaa ttcctcccag
     9361 tgatattact gcaatctcgg ttgtaccccc tcctatatca acaatcatat tgcctatcgg
     9421 ctggtctaca ttaagcccgg cgccaattgc agctgccata ggttcttcaa caagttttac
     9481 cttccttgca ccggcatcat atgctacttc ttccactgct cttctttcaa ttgaagtaac
     9541 tcccacggga acacttatta ctatatttga tttaataaat ctttttttgt taagagcctt
     9601 tgttataaag tattttatca ttgcccttgt gatttcaaag tctgcaatta ctccgttttg
     9661 caacggtctt atcgccctta tggtctttgg agttctctca agcattgctt ttgcatcaaa
     9721 accgactgca catacctcat atgacgtcgt gtttattgca accactgatg gttcatttaa
     9781 agtaattcct ttattgttta catatactaa ggtgtttgat gttcctaaat ctattcctat
     9841 tttctgagta aaaaaatcaa atagtcccat ttatgtatcc tttcatttta aattaagcct
     9901 tcttccttaa aactgaaata tacgccgtca ccgattatta tatgatctaa aactctgatt
     9961 cccaaaattt tgccgctttc atccaacctg tttgttaaaa caatatcctc atggctgggc
    10021 tcgcaatctc cgctggggtg attgtgaact aatattattg aagaagccaa atttacgact
    10081 gcttcataaa atacttcccg aggatggaca attgaactgt ttaagcttcc cactgatatt
    10141 tcagaaacct tttttacgtt gtttttggtg tctaaaagca ttattttaaa atactctttt
    10201 tgataatatc tcatttcttc catcatcatc acagctgcat catcaggaga agaaatcttt
    10261 aatttttcaa tttttaatgt gctcattctt ttgcttattt ctgcaagagc cataagcttt
    10321 gcagccttag cgtcactgat tcccttgtag gatttaaact tttccagact tccttctgca
    10381 atatttcgaa gccctttgcc gccttccttt agaatcctct gagacagcat aacaacattt
    10441 tcttccctcg ttcctgtcct taaaattact gcaataagct ctgagttaga caatgcaccg
    10501 gctccatatt ttaatagttt ctcctgaggc ctgtcatctg caggcaatga ttttatggta
    10561 taattagtat tgtccatttt atcccccact atgcaatatt tgattacagc aagtcgtaag
    10621 aaaaatgttt tgaaagcaaa ttattcagtt ttgcaatagg cagccctttt acattattgt
    10681 agcatccatg tatttcttta accaataatt ctccgtatcc ttgaataccg tatgctcctg
    10741 ctttatccaa atactcatat gttttcaagt atcttttaat taatccttct gtaagctcat
    10801 taaacacaac ttgagttttc tcataatcca ctatcttttt tgagctttct gtatcaatta
    10861 tacaaatacc tgtaaacaca tagtgagttt ttcctgaaag cagactaagc attttataag
    10921 cttctgcttc atcttttggc ttgcccatta tttcgtctaa atatacgatt gtatctgcag
    10981 ctataatcag tgcattttct ttatatttta aatagacatt taatgccttt tcaaaagcta
    11041 ccttcataac cgttgtttgg gggtaatcaa aagaatttac cttttctacg atatcaggaa
    11101 tataagttat tatgtctaca tatttcttca tcatttcaat tcttctcgga gaatttgatg
    11161 ccaatactat tttcttcata ttaatcctta tagttttctg aatattatta ttcctgcaat
    11221 cagaccgatt attcctgcaa cagtcagtct ggcgctgaat ccgaatgtaa tagtgaaaat
    11281 gttcaaatcc aaagttgcag gaccaaaacc aattccttcg ccataattta gtatgggaag
    11341 catatttgaa aatgccttac ctaaaattgt tcctataatt acagatgtaa gtatgagtaa
    11401 tatcaaataa aaagaattgc cttttctcat tacacgcctc tcctccaagt tttaacagtg
    11461 gtttccaagt tgtttgttac aaaaattgct gtatatccta caaagcatcc ggtaaaaata
    11521 cttaccaatg acaatatggg caaataataa tacaagttca cggtagtaaa caatattgca
    11581 gccaccgtta cctgagttgt attatgtgcc attgctccca aaacgctaat cccgattaaa
    11641 ctaaaaatat tgcttaaata cttacatgcc aatgacatta caattgtgct tacgataccg
    11701 ctcgcaatac tgtaaataaa tgtaatggga tttgtagcaa caagcattaa aagaatactt
    11761 cttattataa caacaagaag agcatcctta aaaccaaata caactactgt cgatagtatt
    11821 acaatatttg ataaacctaa ctttgctccg ggagctatgt aaggtaatgg catcatacct
    11881 tctattatgc taagtatcag cgctgccgca gtcaaaagcg ataaaaaaat atacctatgg
    11941 attttattca taatattctc tttctaatca gctatgaaat caatttcact ttcgtcattt
    12001 tcgtctacaa tttttataaa cattctattt ggaatacata caatcatctc gccgtttttt
    12061 gatattttgc cctgatatat gcaaatttta tcgggacagc ttgcttcgtg tatccaaatt
    12121 tgtccatttt caatatgcat tatattataa cctgtttcag attctatgtt aactacatcc
    12181 ttataatcgt cggttaaaac atattctccc accaatttat tgttagaata aatatatgct
    12241 gttttgctat tgctgatcac ggcatcccga ccgccataca aaataaagaa aagactaata
    12301 attattatag atactataag gccaatatca tatttgttga ttttcttatt catagtattc
    12361 cttcgtcata tttaatagat tatttatttt atcaccaatt aattaatatt tcaagaatgt
    12421 atatgtttat atatgtgttt ttaataatta tttaagaaac gcaaaaaaag actctgaaaa
    12481 atagggcttg cagggagaag tatcggttag gcagtactac ttttctatta ccataaaatc
    12541 agttacaagc cgggatagtt tattattctt gtcaaaaccg taataagtag ggaatgttcc
    12601 ttcattccag ccgctggcaa aaacaattat gtttagacct ttttcttcat ccataattat
    12661 atctgcacat gagctgtcac tttgtcgtac cgcatccttt acttttgttt cataatcatt
    12721 taattcttct tcgctcataa tatcaagcat ttccatgacc ttttcatcca tgaaacatcc
    12781 gagcccgttt tcaacttcat atcccataaa ttcgtcgctg ctgaaccctt ttgcggattc
    12841 accttccaat aaagccattt cccatttaac aggcacattt tcattgattc tcagctcggc
    12901 ggcaacagtt tgtttttttc tttttccgca ttttccaaca tatatattga cagtatataa
    12961 tcccggctta acatgctccg aaaaacattc atcatcatac aataataccg ggtctgtggt
    13021 tattattctg ccggtcaaca atttaagttt ccctgcttca attttttcaa tattcatcct
    13081 gccaaatacg gagtctatga tatgtgtatt gccgtttgta atattaagaa catctaagtt
    13141 tatatacatc ttatcacctt acttttttct ttcatttaaa atttttgtta attcatccat
    13201 gaaagtatca atgtctttaa attttctgta aactgatgca aatcttacat atgccacatc
    13261 atccacattt tgaagttcat tcatgaccaa ttccccgata atttcggttg aaacttcatt
    13321 ttgaaatgta ttgtacaatt gtttttcaac cttatccgca attttttcaa tttgctcaag
    13381 acttacggag cgtttatcgc aagcacggat aagaccgttt attattttat ctctgttata
    13441 accctgcctt gttccatctc ttttaattac tacaatttga gtttcttcta ttttttcgta
    13501 tgtagtaaaa cgatgtccgc attctatgca ttcccttcga cgtcttattt taaacccatc
    13561 atcggtaggt cttgtatcaa taaccttgct ctctgtataa ttacattttg gacatctcat
    13621 aaattactcc ttttcatagc aatgacaaat tatttcaaat tactcatgaa tttttcatca
    13681 tcaataacaa atctttcgtg ggttccttct cctatttttt tgtcttcatc ataagctgtc
    13741 acattaaaaa ccaatctcct tctgtctatt tcaataagct ctgcttctgc ccacactttt
    13801 ttgccgactc ttgtagcact catgtgtttt acattaacta aggttccaac agttgaacag
    13861 ccccttggca aaaaattttt tacggcattc atggaagctg cttccattag accaatcatc
    13921 ataggcgttg caaaaacttc agcagcaccg ctgccgaaaa cttttgcggt atcatttatt
    13981 gtaacagttt tttcttcctt gtattttaac ccaactgata gatttaattc cattattacc
    14041 tccaatacct gttcttaccg tattatacaa taaaataata taaaataaaa gaggttttga
    14101 aattaaaacc tcatagacat tggtgccagg caccgccatg gtgcctggca ccgagaaaag
    14161 catgtcacca aacgttctta ctgtcttttc aatgcttcaa ccggcggtaa atttgaagca
    14221 tttattgcag gatatacacc gaacaaaagt ccggaagtca gtgcaacaaa tactgctatt
    14281 ctgatcgaat caagacttac aactgcagaa agtccgtatt ttgccacaat acttattgcc
    14341 caagcaccaa gaacaattcc tgcaacacat ccgattatgc ttacatagac agcttccaat
    14401 aaaaactgaa ttaataaatc ttccttctta gctcctaaag ctcttcttac tcctatctcg
    14461 cttgttcttt ccgtcactgc cacaagcatt atgttcataa ttcctatacc gcctaccaga
    14521 agcgatacag cagcaatacc tccaagtagt accgtcatta ttctgtttgc ctggtcagct
    14581 tcctttacta aattatttaa actagtaatt gtaagcggct cttcaccctt ttcaaagaca
    14641 ttttcttttt cacccggttc ttccggttct tcaggaacag gttcaggcat tggctcctct
    14701 attggcatat ccattcctcc gccttctccc ggaggctgct cgccttcgcc ggtactcggt
    14761 ttaatattgc ttcctaaacc gactttcctt ttatatattc ttccaagctg aaccattgca
    14821 agttccgcat cttcggcact gttggatttt gcccatattt ctaatatatc ctttgttttt
    14881 gctattttca tagcagaagt atagggtatt acaattttat catcgattcc gtctccctta
    14941 ccttctcctt tttctcccag aactcctatt attctgtagt ttataccgtc aattttcatg
    15001 gtatagccaa ccggacttct gccgtttaaa agcgatttag caacattata gcccaaaacc
    15061 acaaccggag acctttgttt tacatgaagg gatgaaaaaa agtttccgct gattacgggg
    15121 tggtccttta tttgtggata actttcattt actcccacaa tattcatttc actttcatct
    15181 cttcgccacc ttacaggtgt gtcttccgta tatatgaccg gagttgcata ttcaatcccg
    15241 gttactcttt ccggcagtat ttctgcttct tctatttcaa attttacatt atcagcattt
    15301 gatttaataa caataacatt ttcacccaag ctttcaaatt gtgctactat agatcttctc
    15361 gcaccttcac cgatgcccat taagctgatt acagctgcaa caccgatgga aataccaagg
    15421 actgttaaaa atgttctaag caaatttgat gttatgccgt ctaatgccat catggcagaa
    15481 aatttcaacc ttacaaacag gttagaaata ttccctcttg cttgcattat catcacccct
    15541 tatttgtttg taccgggcag tattatatcg ctctcaggca cagactgact gggcatcaaa
    15601 tctttagtgc tgccggtaac aacaagctgc ccttcttcta atccgcttat aacctcaaca
    15661 tacatatcat tcattaaacc tgtttcaatt tcaactacct ctacctggtt atcttcctta
    15721 agaatttcaa ctttttgttt tccgttttct tcgaagacag cttcaatcgg tattaaaagt
    15781 gtatctaatg attctccgga gtctacaaaa cagtttgtat tcataccggg ccttaatcct
    15841 tctcgcccct cgactctaat ttgcacgtta tatacagttt ccccggttct gtaatccgta
    15901 taggaatcca atctcattac tttgccatcg aaagtttctc cgggaagtgc atcaaccgtt
    15961 actataacag gcgcatcctg agctatgtaa agaacatcca aatccgaagc atttgtgtag
    16021 atatacattt catcagtatt atacacatcc atcagcatca aggaccactg gtcgctttgc
    16081 tcgtttgctg ttatctggtc accgactctg taattaacgt aagcaaccat tccgtctgtg
    16141 ggagccgtaa ccattaactt tgatgtaagc tcggatattt tatctattct tctgtaaata
    16201 ttttctattt ctttgtatgc attcgttatt tcatctatat cattttgaat aagttgagtt
    16261 acatcacttc cagctatacg gacaagcagg tctccttctt caacaaattc atttgtgctt
    16321 acataaactt cagtagcaat tacattagaa ttttcaccgc catgaagata agaataagtt
    16381 actttctttt catttacgaa tgagtttact tctcctgtat atgataaggc agttacagga
    16441 attccattgt tttcggtata aatagaaaca gtcattccgg gttgtacaag accggggttt
    16501 tcagcttcaa ttgtacctgt atgtacatat gtcataccgt tgggcacctt attcttgttc
    16561 aattttttta tagtcccggg gtagaaaccg tcaaaagcaa gctgggagcc atgttccgtt
    16621 gtctttctat actgcagcaa tactttttga ccttccttca aactttcaaa ctcatatgta
    16681 ttaactttga aagaaatttt caccatactg tcatcaacaa tagtagcaat attgaagttt
    16741 tctacctttt caccctcttc aaccgaaagt tctgtaattc gtcctcgaat aggtgctcta
    16801 aatactattc catcataagc attaacctga cttatatctg taacatcctt atttaatatt
    16861 ttttctaagt tagatatttt tgcctgaata tcttcctgtt tcttttgaat gctgtcggtc
    16921 aattcagtca aaatgtcacc taagtttacg gcagataaac gaactaaatt atctcccttt
    16981 ttaatcattt ggttaggctc aacaaacact tcttcaacag tatatgttac cgatacgctc
    17041 ataccgtttg aatcagttat accttcaggt cttggtgctg ttattgagcc tccccagtta
    17101 ccgctaagct gccctgataa attaactcct tgttttatat ctcctctttc aactgccttt
    17161 gttgaatatc ttggacctgc agatgcttcc gtatctttag gaataagcca ctttgccgtt
    17221 aaatatccgc cgaaaaatac taatcctaat actattgcta tcgatataat ttttttatac
    17281 atttttaacc tccactacat catcagatac tatttgacca tccacaagtc ttatgattct
    17341 tgagccgtat tctgcaataa attcttcgtg agttacctga acgacggtca ttgagttttt
    17401 ttcatttaat tctacaaata attccataat caattcgcct gattttgaat ctaatgctcc
    17461 tgtaggctca tccgctaata taattgacgg atttcctacg atagccctgg ctatagctac
    17521 cctttgctgc tggccgcccg acaattgagc aggcaaatgt gtcattctgt ccttcagtcc
    17581 taccagctcc aatgcatttt cggccagctc tcttcgtttt ttatcgctca ttcccctata
    17641 tattaaagga atttcaacat tttttattgc agtcatcctt ggcagcaaat gaaagctttg
    17701 aaatacaaat ccgataaatt ggttccttac atctgacatt ttagaatcgc ttaatttgtc
    17761 aacctgattt cctgctaatt catatgttcc gcttgtagga tggtctaagc atcccactaa
    17821 gtttaacagt gttgattttc ccgagccgga aggtcccata attgaaacaa attctttttc
    17881 tttaatattg aaggaaatgt tttttaaagc ttccacttga atttgacctg atttataaat
    17941 ttttgaaaca ccttgcattc tgattatatc catcttccac ctctctttta cacatcagtc
    18001 catatattag acgtataaac ttaagaaaaa gttcagtaaa cattaataat tttgtaaaaa
    18061 aaagaacgac cgggtcgttc caatagtcag tttgattaat tatttgatta cgtcaataaa
    18121 caaggaatca atccacactt tattttcacc ctcatattcc aaataaatat acaagacatc
    18181 agcttccgtt acattaatag gtactgtaaa ctgtgaccat atatcatcat atatattaac
    18241 caatgaatga aaatttgact ttataacacc atctttatat gcttcaaaaa ctacccgagc
    18301 ttgagtatca tattgtgatt tgatttttcc tgaaacccct atttgtttca taagcatata
    18361 gttgaaatta tcatctttga aatcaacagc tgcaaacagt gctttaactt tatcctgtcc
    18421 actattggcc actgttaagg aagagcttcc ttccacatat atatcggtat ctttttgtgc
    18481 ttttgctcct tcatatccga tgctctccaa cagctcttca ctgaaatgaa tatctgttga
    18541 ctcgtaaaca ggttcttctt caaccggtgt ttctgaaggt gcctcctgca aaggtggttt
    18601 aacgttgtta attgcttcgg cctcatcatt tttattgaat ttttttatta taaaattacc
    18661 caaaagaagt accataacaa ctgtaagcaa tgatacagcg gctcttttga tatctttatt
    18721 tataagtgct ttctttttag ctggttgatt atcttccaaa taaatattga gataatcgtc
    18781 cgattcttca ttgtgtatta caagagacgg ttcctcaggt tgttcaatat ccacagtatt
    18841 attaataatt tcttctccaa aaagcatatt aaaaataggc gaccttttta attccaccaa
    18901 agcatcgttt ggaaaaacat attctcttgt aaggcatctt actattattt tcaatatgtc
    18961 aggaggtatt gagtcggata tgttataatc aataatttct attccggaaa aaatcatatg
    19021 tataatattg cccaatcttt taaatgtttc attgtctgct atatcatatt cctgctcaaa
    19081 tatcagattg cagtcaacaa tcaaatcatt attatcatcg atgaacaatc ggtctatatc
    19141 caaaattttc tgctgtacta catcggtcat attgtaaata tttgaggcta aatttattga
    19201 cttttctgca agactgaact gttgtttcat ggtgaggtta ctgtttgcta aatctgttaa
    19261 ctttctgcaa tctgtttcta aagattttgt aaatatataa attctatcct ctgtattgta
    19321 gcactctaat atattggatt taatcttttt taaatcgttt atgtttatta aatttataac
    19381 ctttttgctt gttattatat tggagtagaa aattgcaccc gtatcatcgt ccttgcattc
    19441 taaaattctt tgaaaagcat tttcataaat tgtacggata atcttgtaat tattattcat
    19501 atacagacct ccaaatatta ttaactatat attaacaagt atttcgacaa aaataaagac
    19561 atttttgtat tattttacaa ataaataagg atgagttatt tctcctcatc ctcgtcaatt
    19621 tcatattcaa cattttctat aatatctttg tcttcatgtc ttttatcaaa ataatatttc
    19681 ttggttttat catagactat tgttaataaa aatccaagca ttaccaaatc gtctactatt
    19741 ccgagcccca atattggaaa aggtatcaga tccgcaggac tcaatatgta aataagaggt
    19801 attataaccc accacttttc ccttatggga atatttttat caaataaaaa ttttattata
    19861 tataaaattc tgcccatatt cgcatcctag attatgaact caacattagg aagcttcctc
    19921 tgcagcttag cgataaaatt gtttgatgga ttgttcgagt ggcccaatac aatatgagtg
    19981 caatttattt cttccgcata ttccactatt gtctttatta catccttgga tcttttaaca
    20041 actagattag cagcacccac atctttagta atttcaaata aatattctaa cgcatctccg
    20101 tcgcttaaac catacaaaaa cttgtcattt tcatttacaa catgtataac atttaaacag
    20161 ctgccagctt ctccttttat cagttccact ccttttttta tcaatttttc gcttgttttc
    20221 tgtcttgtta cacataccaa aacacaatta ggcataattt atctccttaa tttggcggga
    20281 atatgtggga attgaaccca cccaggacga ttccgccccg caactgattt tgaagaccag
    20341 gaggcacacc agcacctatc tatccccatc acaaatatat taccatatcc acctgataaa
    20401 ttcaagctga ttttactatc gtaatgactg aattaaaaaa agctgcataa tatgcagcag
    20461 tatgggcagt acgaggacat ttaattcctg tttctaaaat aatatcctac tccccatttt
    20521 gtgagtatat actcatatgc cttgtttttc gtttctattt tttccctcaa tcttctaaca
    20581 tggacatcaa cggttctcaa atcaccgtag tattcataac cccatattat ttctaaaagt
    20641 tcctccctgg aaaatacttt cccggggttt gttattaata aaagcaacaa atcaaattct
    20701 ttcgcggtaa gatttatttc ctgcttatga agacttactt tcctgcctaa ggcattaatt
    20761 ataaaatcat ccaccaccaa agtctgttca tttgcttttt ggctcgtacc tcttgtcctt
    20821 cttaaaattg ctttcattcg tgctttaagc tctaaaatat taaagggctt ggtaatataa
    20881 tcatcagcac catattccaa tcctaagatt ttgctcatat catcattgtt tgctgacaaa
    20941 atcaaaatcg gaacattcga tatttctcta attttttgac aaacttctaa accatttgtt
    21001 tgaggaagca ctaaatcaag tattataata tcgtaatcag tcgttgtaat tttttttaaa
    21061 gcttcattgc tgtcaccggt agattccact tcataattat cctgctcgaa actatattta
    21121 agattcttaa tggttaaaag ttcatcatca acaatcaaga ctttcattta ttacgctcct
    21181 taaatttgat tgttacaaaa aattaaactt ttaataatct tacataaaaa aacttatttt
    21241 gtcaagaaaa atattaattt tatgtcaaat ttagtagtat tctatcattt tctattcttt
    21301 ttgtaagttt taacatatcg aag
//
LOCUS       DAOPUE010000028        20257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2667_length_20257_cov_9.597664, whole genome shotgun sequence.
ACCESSION   DAOPUE010000028 DAOPUE010000000
VERSION     DAOPUE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 20257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20257
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_20257_cov_9.597664"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..399)
                     /locus_tag="PKH42_04125"
     CDS             complement(49..399)
                     /locus_tag="PKH42_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOA19652.1"
                     /translation="MAEPVKANITLDLLDKIDIRVGMIISVEDVAKSDKLVKLTVDFG
                     EFTRNILVGMKKERDNPKEIEGKQALFVVNLAPKKMAGEISEGMLFDIGYADGITPVL
                     AQPEKPVPNGSRAG"
     gene            complement(606..1217)
                     /locus_tag="PKH42_04130"
     CDS             complement(606..1217)
                     /locus_tag="PKH42_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19653.1"
                     /translation="MIERKLLKILSLSLVLSLSLIGFVFAETKENLDEPVSIQITAVD
                     EEMYEEQADIDKLLFEQNYANDLELKGIYITHTSTVDDYVEIGITPYTPENADAVVRL
                     LGKDNVKVVEGVLAEILQYSTDNPETIDPAELADDSQIVDVTADAEDISQPADEDVVE
                     DAEIISAPAEKEVEKNGISPFNILGLSAFVIGIVVVLFKKKSA"
     gene            complement(1326..1691)
                     /locus_tag="PKH42_04135"
     CDS             complement(1326..1691)
                     /locus_tag="PKH42_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOA19654.1"
                     /translation="MKFTFNHFNFNVLDLEKSMKFYEEALGLKEVKRKEAEDGSFVIV
                     YLGDGKTNFSLELTWMKGRTEPYDLGEQEFHLAMAADNYDEAFKKHEQMGCIEYINEK
                     MGIYFIVDPDGYWVEVIRG"
     assembly_gap    1878..1977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2071..2997)
                     /gene="fmt"
                     /locus_tag="PKH42_04140"
     CDS             complement(2071..2997)
                     /gene="fmt"
                     /locus_tag="PKH42_04140"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOA19655.1"
                     /translation="MNVIFMGTPGFAVPTLEKLHESKHNLVLVVTQPDKPSGRGKKLK
                     KSEVKETAEKLGLNIFQPDKIKKQENIDLLKSYNPDVIVVVAYGQILNKEILTLPKYG
                     CINVHASLLPKLRGAAPLNWALINGETKTGITTMQMDVGLDTGDMLLKSEVEIDENIN
                     VGELHDILMHKGAELLIETLDKLEKNELTPKKQDDSLSSYAPILNNENRKINWNLPAK
                     SIHNLIRGLSPWPAAYFTLDEKTVKVYSSSYINNDSDYEPGYVIKANNEGIFVKAKDG
                     IVILKEIQMPGKKKMPVEAYLRGNKFPEKIIL"
     gene            complement(3006..3464)
                     /gene="def"
                     /locus_tag="PKH42_04145"
     CDS             complement(3006..3464)
                     /gene="def"
                     /locus_tag="PKH42_04145"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOA19656.1"
                     /translation="MALRNLRYEGDEILRKKSREVPLVDDRIRMLMEDMVETMYKYDG
                     VGLAAPQVGILKRVIVIDVEDGKVYKMANPNIINESGEQSAQEGCLSVPEKKGIVSRP
                     MKVIVEYTDENNETVKLEAEGLLARAICHEIDHLNGVLFIDRVTDDVIEE"
     gene            complement(3477..5681)
                     /gene="priA"
                     /locus_tag="PKH42_04150"
     CDS             complement(3477..5681)
                     /gene="priA"
                     /locus_tag="PKH42_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOA19657.1"
                     /translation="MYNTFVQVILNNISKSTDKIYTYGVPDALKSFVDEGKRVRINFG
                     RGKNIIDALIVSVDLTCDYPIEKIKPILDIIDEKPIVTKEMIKILFWIRERYLCKYSD
                     AMRLMIPSETKSIYEKHIRLKEDKEISQYKINKTENQIKVIEYLKNHKSASIAQLMDR
                     LNISKAVINNLIKRDIIEESLVFDYSNQREFQSDEPLCLNEEQKNACDTILNSDKKVF
                     LLHGVTGSGKTEVYMSIIEKYLKEGRQSIMLVPEISLTAQTIERFEKRFGNKIAVFHS
                     KLSKKDRYIQWMRVYKKEADIVIGARSALFAPLNRLGVIVIDEEHEDSYISTTSPKYD
                     TMEVAVKMAYMMDGKVILGSATPSLNTYYMAMKGQIELIEMKNRVKDAEMPEMKIIDM
                     SAELDKGNNTIVSEDLYESIKNSLKQNEQVILFLNKRGYSGFVSCKKCGYVVRCQRCD
                     VSMTYHVVGNIMKCHYCGSAKKMIYTCPKCGSEKIEQFNAGTQHVEKLINQLFPYAST
                     ERMDLDSMTDTESYENIYNDFKNKKIDILIGTQMLAKGFDFPNVTTVGVLSADAILNL
                     PFYNASEKTFQLITQVSGRAGRGDKRGNVYIQTYEPENFIINAAKNNDYELFLHEELK
                     LRKEFAFPPFINIINICLISKIEELVVKTANEKYLEVKETVKEMVKERSLLLYRPVPH
                     NIYKVNDEYRINLYIKVSNHSLSELKKILRTVYMEKDIENIKVSININTDTI"
     assembly_gap    5820..5919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5920..6514)
                     /gene="gmk"
                     /locus_tag="PKH42_04155"
     CDS             complement(<5920..6514)
                     /gene="gmk"
                     /locus_tag="PKH42_04155"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOA19658.1"
                     /translation="MDEGLLVVISGPSGAGKGTICAQLLLEMSYLKVSVSVTTRKPRA
                     GEVDGISYYFIEEEEFINRINNDEFLEYAKVYGNYYGTPKEEVFKQLKAGNDIILEID
                     IQGALQVKKNYPMGVFIFILPPSLEELKSRIEGRGTDSKEVILRRMESAYEELNYAFQ
                     YDYVVVNDHVDTATEKIKHIILAEKNRVIRKKEIISKI"
     gene            complement(6524..6772)
                     /locus_tag="PKH42_04160"
     CDS             complement(6524..6772)
                     /locus_tag="PKH42_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOA19659.1"
                     /translation="MSNTINIGFGNIALANRIVAIVSPESAPIKRLIQETREKNKLID
                     ATYGRKTRAVIITDCDYIILSAIQPDTMALRFENHNIK"
     gene            complement(6782..7669)
                     /locus_tag="PKH42_04165"
     CDS             complement(6782..7669)
                     /locus_tag="PKH42_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOA19660.1"
                     /translation="MANIFSMTGYGKGEHNDGKRSITAEIKAINNRYCDINIKTPRHL
                     RIYEDNIRKILKNGIQRGRIDVYINIEYISESDTVILPNLALAKQYMDAIEKIKSELG
                     INGNPSLDTIIKFQDVLIAKEDDADDDELRICLENAMNEAVKNLLTMRVKEGIQLKDD
                     ISLSLVKIRELMEVISENSHTIVEDYKIKFEDRIKELLGNTHELDENRLYNEIVIYAD
                     KSDINEEIVRFNSHMVQLSDALDSGGAIGRKLDFILQEANREINTIGSKVSSLDIIQV
                     VIEIKNLLEKIREQVQNIE"
     gene            7966..9726
                     /locus_tag="PKH42_04170"
     CDS             7966..9726
                     /locus_tag="PKH42_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOA19661.1"
                     /translation="MSLDGIFLNSIINDLYKKLIGGRVDKIYQPDKNEIVISIRSKGE
                     NYKLLITAISNSPRLHLTNVIRQNPSEPPMFCMLLRKHLTSAHITNIKQINFDRIVEI
                     SFECKDEFGTAVNKSLITEIMGKHSNIIFINEDKTIIDAIKRVAENISSVRQVYPGLK
                     YVIPPGSDKLNPLTVTKDLFMKEIESTNNGIYIYNFLYKNFLGLSPFISREICYLTNL
                     NESTYLGELSEEKKEEIWNVFSKIMNKIKQNDFSFNIFYDENMNQYGFYCLEVEYLKD
                     YERKNFLSPGELLDEYYYELDNKNKINQRVASLIKNINTKIDRNRKKVEKQRNELLNA
                     ENRDKYKIYGELILANLHKTPENHKLRVINYYDPEQKEITIPLDPRFNLPQNSQKFFK
                     RYNKLKKAEEELEKLIDITLNEIKYLENILFSIEECETIDDLNDIYSELISEGFMKRK
                     SKADKSNKTKPITAFISSGGHEILVGKNNTQNDMITFKIAKNEDYWFHAKNMPGSHVI
                     IRTKGDELTDEEFIEAAKIAAYYSKGKNSSTVEVDYTKKSNIKKPPNAKPGFVIYDTN
                     YSMLVKPNIEGIKRDSGDVS"
     gene            complement(9771..10301)
                     /gene="pyrR"
                     /locus_tag="PKH42_04175"
     CDS             complement(9771..10301)
                     /gene="pyrR"
                     /locus_tag="PKH42_04175"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOA19662.1"
                     /translation="MKIKALIMDEKAIERATFRIAHEIIERNKGVENIVLIGIKTRGW
                     PLSIRLAKKIEEIEGSKVPVFPLDITYYRDDVEKDDRAPLSVKSFDFDIKNKTVVLVD
                     DVLYTGRTVRAALDAIVDQGRPNNVELAVLIDRGHRELPIRADFIGKNVPTSRSERIS
                     VRLEETDKINQVVITE"
     gene            complement(10358..11275)
                     /locus_tag="PKH42_04180"
     CDS             complement(10358..11275)
                     /locus_tag="PKH42_04180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOA19663.1"
                     /translation="MEISDSEKEIILVSDEENKRIDVFISEKSGESRNFVQKLISDGN
                     ITVNEREVKANYKVKINDTIKIIIPPPEILDVKAENIDLDIVYEDNDLAVINKPQGMV
                     VHPAPGHYSGTMVNGLMYHLKNLSTINGVLRPGIVHRLDKNTSGLILVAKNDRSHNFL
                     AKCLKEHTINRIYYALVKGNIKKDEGEINAPLGRSEKDRKKYTVTNKNSKEAITNYWV
                     VERFGKYTLLKLKLKTGRTHQIRVHMKHIGHPVVGDDVYGNVNEFGLEGQLLHSKILG
                     FIHPTTGKYMEFESELPDYFVKVLNKIKS"
     gene            complement(11391..11855)
                     /gene="lspA"
                     /locus_tag="PKH42_04185"
     CDS             complement(11391..11855)
                     /gene="lspA"
                     /locus_tag="PKH42_04185"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOA19664.1"
                     /translation="MSIIAVFIIIFSVVFDQLSKYWATEVLKNGESIKIIGNFLRFTY
                     AENRGAAFSILQNQRVFFLIITIIMLIILGIVYFTNRNLSKLSRLSMAMIAGGAIGNF
                     IDRYRMGYVIDFIDVRFGTFYNFPVFNIADSFVVCGTILMVILILFNKFETS"
     gene            complement(11867..12649)
                     /locus_tag="PKH42_04190"
     CDS             complement(11867..12649)
                     /locus_tag="PKH42_04190"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOA19665.1"
                     /translation="MKSLEKYYDFIRDDEIKNTVKKIQDKASYVNKNYIAAITEFINP
                     YVVKISIPVIKSYDIKFEIFPSYENCERKVFILYPQYSEIDENEFISGIRITNKSKFK
                     KLSHKDYLGVLMSLGIERSKLGDIYVCDDYGDVVVHSDIADYIIYNLDKIGHNKIEAE
                     KINLSQVNFKEQEHEILNIITSSMRIDNIVKHLTKLSREKASFIIKSGDVKINHIPEY
                     KVSTELKEEDILSIRRAGRFKIYKIIGTTKSGKLKIQIKHYI"
     gene            complement(12676..12939)
                     /locus_tag="PKH42_04195"
     CDS             complement(12676..12939)
                     /locus_tag="PKH42_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOA19666.1"
                     /translation="MFYIIQLALASLLRIIYYLILVRVILSFFPTLQTSRISYFIYQM
                     TEPVLAPCRAILDKLGLGMGMFDFSPILALLLLNILQRLIYAI"
     gene            complement(12949..13386)
                     /locus_tag="PKH42_04200"
     CDS             complement(12949..13386)
                     /locus_tag="PKH42_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOA19667.1"
                     /translation="MSMMEKVKDFIGITDLEEDYEEEDLVSEGTERNKNERMGTNTKR
                     NNVIKVHSNTDMKVFICEPEKYEDCTKAVDEIKNRKVVVLNIEGMELEDQKQVFEFIK
                     GAIYALEASIQKVSNGIFVLAPNNVQIDGRLSDRYERNYYYGK"
     gene            complement(13387..14091)
                     /locus_tag="PKH42_04205"
     CDS             complement(13387..14091)
                     /locus_tag="PKH42_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOA19668.1"
                     /translation="MSIRENIDDIVKRIEDTCKKVGRNPNDITVIAVSKTVESERARE
                     AFEAGINNLGENRVQELVKKYDELKDLDIKWHMIGHLQKNKVKYIIDKTVLIHSVESL
                     SLAEEINKRAEKNNLIANVLVELNIGEEDSKFGIKEESVYDFILSLEKFENLRVVGLM
                     TVAPFCENPEDVRWVFKKMKYIYDKISTMNLRNTEMKYLSMGMTNDFEIAIEEGSNII
                     RIGTAIFGARKYKEEE"
     gene            complement(14101..15327)
                     /locus_tag="PKH42_04210"
     CDS             complement(14101..15327)
                     /locus_tag="PKH42_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOA19669.1"
                     /translation="MARKNFGLKNFFLSFALVVIAIYVFQNIKSPADFMIAENGILED
                     VIKAEGIVVKDEKVYSATLDGNVLYYYEEGTKIKQGQLIADINTDADSVEVNRQIAEI
                     QAAINKLENSSQVLTKENFTAYQNELQISILNNDLQNMYGIVEQMTNNDFYGSKYEDF
                     SISQLIEMKNSLANSISTHKVPYYSDRAGIISYKTDGLEGIYRFENVMDMTPSSTLRR
                     EYSLKDKRESVQKDEAFYKIIRNFEYYIIVTINNEKARLFEENKYIKVRILSDGEQHI
                     VWGYIEKINYGSEESVLILYFDDYFYKVYDKRYVNIELITAAYEGLKIKNESLIEVNG
                     IKGVYVQDASNIIKFFPVQILGSNEEYSVVFPGDYIGINERNIITIGEKRYPTIKIFD
                     KVILEPDKVYEGQIAD"
     gene            complement(15561..16688)
                     /locus_tag="PKH42_04215"
     CDS             complement(15561..16688)
                     /locus_tag="PKH42_04215"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOA19670.1"
                     /translation="MERDKYFEEYYEKKRNNISFITLKKGAAVNYKDKTYAAKKELPV
                     PVRIEKLLEDINKQNEMDGITLNNIIDGIIYILGTDRNFEYIQDYKEMFKELNFELIP
                     YIIYCINNDIKTEDGVVYGRALINSEENEKTCFIYASCLEKMGMEYHEKGNDSSRYFL
                     EEAHFYYEKCLDYNDKFSLAYYKLGYYYKRKQQYVKAELIWQKHQELDDNDLRIEEIR
                     NELIQLKPFVDYEKGYNLVLKERPDEALDLLLPLVKDFGGWWNLLFFIGLAYRSKREY
                     DIAETYFENVLKIDSTQKETLNELGLCKICTGKYVEAAELFSTLLSLDPGNCEVFCNR
                     AVAYLYNNQLERAKEDIQTALKINPKDTVALGIKEELEKKH"
     gene            complement(16675..18387)
                     /locus_tag="PKH42_04220"
     CDS             complement(16675..18387)
                     /locus_tag="PKH42_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOA19671.1"
                     /translation="MKILLVTLDSKFIHANLAVRYLKKYCRGFDIVIKEFTINQKIEY
                     ILDEILAEDADLICFSCYIWNIDYIKDISYIIKESNNKAKILYGGPEASFEIKELMEN
                     NPFIDYVIFGEGEETFKEFLEEIQNTNSNLYKIRGLAYKENNDVIINEGREPIDNLDI
                     INYPYEVDDKFANKIIYYESSRGCPFSCSFCMSSIDKKMRFFSMDRVKKDLKMLLNTK
                     ARQIKFVDRTFNADFRRSMEIMQYIVENNRNNMTIHFEITADIINDEFLSFIAKMPVN
                     MFQFEIGVQSLNEDTLREINRHMDKDKLCRVIKEIGKNKNVHMHLDLIAGLPYEDYES
                     FKRSFDGIYNLDAEKIQLGFLKVLKGTKIYEDREKHQIKYGLKAPYEVICTKYISLKE
                     LLKLKNIEELLDKYYNEKYFSKSLKYITCYIFPDSAFEFYSSFSDYWQKYNLYNYAHS
                     RKELYKILYDYIVHMKRKNDEFVQALKFDYIFNNQYEELPDYLNRETEDKFKSIKRQL
                     ANDIEFKNKYFPSMKGKIINDFRIAEIGGKIVLFVYRDRENIFNRCDTYVVNNIEEIV
                     YGKR"
     gene            complement(18384..19238)
                     /locus_tag="PKH42_04225"
     CDS             complement(18384..19238)
                     /locus_tag="PKH42_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19672.1"
                     /translation="MQYKINKMLNFCFDDIQKIDFQLFLITVKCLIPYDDKIGCLFDK
                     DRYKKEIELFSSYMNGNDETIDYWLRNKEASNLDDNLLEYKIIPLALSNTVWENLTDE
                     LIKVVLFYTINKNAILNALLISSAVFDFMTGSDIDSIEAAARERLINFSVKEFLIKNQ
                     INADKNYIIDFEKERIKMIAKPILFDNNFINGFKVLNYIFEKSPEHLTENNSDIIDSF
                     SVYLYKLRKGLISPEKLIIPDKIPEIKECLKYSTFSHPLLGRCKVIKRTEKEAIVRNK
                     LGLMRIRI"
     gene            complement(19417..20064)
                     /locus_tag="PKH42_04230"
     CDS             complement(19417..20064)
                     /locus_tag="PKH42_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOA19673.1"
                     /translation="MKRVNYLTFLIFLCIFSYFMMINTSMTAIAEDNQNYYKFSQDKI
                     IKSIIHDYRDSFIREKELIRYTEELTGLDYEHCRFLIKESKEKYIDPFIVLGLIKRES
                     DFNPKAKGVGGELGLGQLMENTAQIIAMNLGYSYNNEMLLDPVFNLKLTIAQLSYLNK
                     VYDNDMHKTLTAYNRGQQGLKNYLTTCDGRGVSDYSTKVLQFASIYKEEFENLKY"
BASE COUNT         5943 a         3871 c         2675 g         7568 t
ORIGIN      
        1 tttcatgttc tgcctcctta agcaacaata atccggtaat ggattagatt aaccggctct
       61 cgaaccgtta gggaccggtt tttccggctg cgccaaaaca ggagttatac cgtcggcata
      121 tccaatatca aagagcattc cttcagaaat ttcaccggcc attttctttg gagccaaatt
      181 aaccacgaat aatgcctgtt tgccttcaat ttccttagga ttatctcttt ccttcttcat
      241 cccgaccaat atgtttcgtg tgaattcacc gaaatcgact gttaacttta ccaatttatc
      301 tgattttgca acatcttcta ccgatattat cattcctacc ctaatatcaa ttttatcaag
      361 caaatctaaa gtgatattag cttttacagg ctcagccata ataacctcca tatacttcat
      421 tctgaataac taacgctttc attttacaac tcacagtgat taaaatcaac cataactata
      481 tatttaaaaa gacgcttttt ctctaatatt gttccataca atacaggtta tcatgctttt
      541 aaacttacat atgctatgaa aaaacgacag gttattcacc tgtcgtctgt ttagctttct
      601 ttagtttatg cacttttttt cttgaatagc actacgacga ttcctattac aaatgcactt
      661 agccctaata tgttgaatgg tgatattcca ttcttttcaa cttccttttc agcaggagca
      721 cttattattt ctgcatcctc aactacatct tcatctgccg gctggcttat atcctcagca
      781 tcagcagtta catcgactat ttgcgaatca tctgctaatt cagccggatc tatagtttcg
      841 ggattatcag tactgtattg aagtatttct gctaatacac cttcaactac cttaacatta
      901 tcttttccta acagtctaac aacggcatcg gcattttccg gtgtatatgg tgtgattcca
      961 atttcaacat aatcatcaac agtgcttgta tgagtaatat atataccctt taattctaag
     1021 tcattagcat agttttgctc aaataataat ttgtcaatat cagcttgttc ctcatacatt
     1081 tcttcatcaa ccgcagttat ttgtatagaa accggctcat ccaagttttc tttagtttct
     1141 gcaaaaacaa atccaattaa tgataatgat aaaactaaac ttaatgataa tatttttaat
     1201 aattttcttt caatcataaa taacctcttg taatttattt tgaagtacca cggcctatgg
     1261 gatttttact tctgataaat tagacgttat ttttttaatt ttgtttcatg gtaatttttg
     1321 ataaattatc ctcttatcac ttctacccaa tacccatccg ggtctacgat aaagtatata
     1381 cccatttttt cgtttatgta ttcaatacag cccatttgtt catgtttttt gaaagcttcg
     1441 tcataattgt ccgcagccat tgcaagatga aattcctgtt ctcctaaatc ataaggctct
     1501 gttcttcctt tcatccaagt caactctaat gaaaagtttg tttttccatc acctaaatat
     1561 actattacga agctgccgtc ttctgcttct tttcttttta cctcttttaa acctaatgct
     1621 tcttcataaa acttcatgct tttctctaaa tcaaggacat taaaattaaa atgattaaat
     1681 gtaaatttca taattacctc actagtattt tttatattaa taaagaattt agcagtcaaa
     1741 ctttctactt atctttcatt tattttgtgc caatagctat agctatgacg gctaaaataa
     1801 gcacggctgc tacaataacc cataacggta cttttttcaa caataaaata gtttttttaa
     1861 tattcataat atcaccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt
     1981 cattatatta ttttggggca tccgtaactc gtccctacat tgacttgtcc tgtcccctcg
     2041 tcatttgatg acagtacccc cgtccccttg tcataagatg attttttcgg ggaatttatt
     2101 tcctcttaaa tatgcttcaa ccggcatttt ctttttgccg ggcatttgaa tttctttaag
     2161 aattacgata ccatctttgg ctttaacaaa gattccctca ttatttgctt ttattacata
     2221 accgggctca taatctgaat cattatttat atacgatgag ctgtacactt ttacggtttt
     2281 ttcatccaga gtaaaatatg ctgcaggcca aggagataat cctcttatta agttatggat
     2341 actctttgca ggtaaattcc aatttatttt ccggttttcg ttgtttaata tcggagcata
     2401 gcttgaaaga gaatcatcct gctttttcgg tgttaattca tttttctcca atttatccag
     2461 tgtttctatc aagagttctg cccctttatg cattaaaata tcatgaagtt caccaacatt
     2521 aatattttca tcaatttcaa cttctgattt taaaagcatg tcacccgtat ctaagcccac
     2581 atccatttgc attgtagtta tgcctgtttt ggtttctccg tttataagag cccagtttaa
     2641 aggtgccgca cctcttaatt tcggcagcag tgaagcatgg acgttgatgc agccgtactt
     2701 cggcaatgtc aaaatttcct tgttcagtat ttgtccgtaa gcaacaacaa ctattacatc
     2761 aggattatat gattttaata aatcaatatt ttcctgtttt tttattttat caggctgaaa
     2821 aatattaaga cctagctttt cagccgtttc cttcacttct gattttttta attttttccc
     2881 tctgcctgac ggtttatccg gttgagttac aaccaagaca agattgtgtt tgctttcatg
     2941 tagtttttct aaggttggaa ctgcaaaacc cggtgtaccc ataaatatta cattcataac
     3001 atcacctatt cttcaattac atcatcggtt actctgtcta tgaacaatac tccgtttaaa
     3061 tggtctattt catggcatat tgctcttgcc aataaacctt ccgcttccag tttaactgtt
     3121 tcattatttt catctgtata ctcaacaatc actttcatgg gtctagaaac tatgcccttt
     3181 ttctcgggga cgcttaagca tccttcctga gctgattgct cccctgattc gttaataata
     3241 ttaggattag ccattttata taccttgccg tcttccacat caattactat tacacgtttt
     3301 aatatcccta cctgtggagc tgcaagtccg acaccatcat atttatacat ggtttcgacc
     3361 atatcttcca ttagcattct tattctatca tcaacaaggg gaacttccct gctttttttt
     3421 cttaatattt catcgccttc atatcgtaaa tttcttaatg ccatttttga accctcctaa
     3481 attgtatccg tgttgatatt tatgcttacc tttatatttt ctatatcctt ctccatatat
     3541 acagtcctta gtattttttt gagttccgac aagctatggt ttgacacctt tatatataaa
     3601 ttaattcggt actcatcatt aactttgtat atattgtgag gaaccggtct atacaacaat
     3661 agacttcttt cctttaccat ttctttaacg gtttctttta cttccaagta tttttcattg
     3721 gctgttttta caaccaattc ctctattttt gatatcaaac atatatttat tatattaata
     3781 aatggcggaa atgcaaattc ttttcgcaat tttaattctt catgcaaaaa tagctcatag
     3841 tcattgtttt ttgctgcatt tattatgaaa ttttcaggct cgtatgtttg aatgtacaca
     3901 tttcctctct tgtctcccct gccggctctt ccgcttacct gtgttataag ctgaaatgtt
     3961 ttttcgcttg cattataaaa tggtaaattt agtatcgcgt ctgccgaaag aactcctacc
     4021 gtagttacat tcgggaagtc aaaccccttc gcaagcatct gggttcctat taatatatca
     4081 attttcttat tcttaaaatc attatatata ttttcatagc tttctgtgtc agtcattgaa
     4141 tccaagtcca ttctttctgt ggatgcatat ggaaataatt gatttatcag tttttcaaca
     4201 tgctgtgttc ctgcattgaa ctgctcaatt ttttcgctgc cacactttgg acaggtgtaa
     4261 atcatttttt ttgcacttcc gcagtagtga cacttcatta tatttcctac aacatgatag
     4321 gtcatggaaa catcgcacct ctgacacctc actacataac cgcatttttt gcaggatacg
     4381 aatccggaat aacctctctt gtttaaaaat agtattacct gctcattttg ctttagagaa
     4441 ttttttatac tttcgtataa atcttcactg actatggtat tgttgccctt gtcaagctca
     4501 gctgacatgt ctataatttt catttcaggc atttcggcat ctttaaccct gtttttcatt
     4561 tcaattaatt ctatttggcc tttcattgcc atgtagtagg tatttaaaga tggtgtcgcg
     4621 gagcctaata tcaccttgcc gtccatcata tacgccatct ttacggcaac ttccatggtg
     4681 tcatactttg gagatgttgt tgaaatatag ctgtcctcat gctcttcatc aattactatg
     4741 acacctaatc tattaagggg ggcaaataag gcagagcgtg ctccaattac tatatctgcc
     4801 tcttttttgt aaaccctcat ccattgtata tacctgtctt ttttacttaa ttttgaatga
     4861 aatactgcaa ttttgtttcc aaaccttttt tcaaatcttt caattgtctg cgccgtaaga
     4921 gaaatttcag gaacgagcat tattgactgt ctgccttctt ttaaatattt ttctattatg
     4981 ctcatataga cttctgtctt accgctgcct gttacaccgt gcaagagaaa tacttttttg
     5041 tctgagttaa gaattgtatc gcatgcattc ttctgttctt catttaagca taagggctca
     5101 tccgattgaa actctctttg gtttgaataa tcaaaaacca aactttcttc tattatatct
     5161 ctttttatta agttatttat cactgcctta gaaatattaa gcctgtccat taattgagct
     5221 atgctggcgg atttgtgatt tttaagatat tctataactt ttatttggtt ttcggttttg
     5281 ttaatcttgt attgggatat ttccttatcc tctttcagtc tgatatgttt ttcgtaaata
     5341 ctttttgttt cggaaggtat catcaatctc atggcatcgg aatacttaca taaatacctt
     5401 tcccttatcc aaaaaaggat tttaatcatt tcttttgtta caataggttt ttcatctatt
     5461 atatctaaaa taggcttaat tttttcaatg ggataatcac aggttaaatc aacggatact
     5521 attaatgcat caataatatt tttccctctg ccgaaattaa ttctgaccct ttttccttca
     5581 tcaacaaagc ttttcaatgc atccggtact ccgtatgtgt aaattttgtc tgtgcttttg
     5641 gaaatattat ttaaaataac ttgtacaaaa gtattataca tattttctcc gctcaactaa
     5701 aattaaataa tcagtccttt gactgattat ctgactattc ctgtgccgtt gtttcatcca
     5761 tgtctttttt ttcatctgtt gtttcaacag ctgtctcctt atttgccgtc aatgattttn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gattttactg attatttctt
     5941 ttttccttat tactctgttt ttttctgcca atattatatg tttaattttt tctgttgcag
     6001 tatccacatg gtcattcacc accacataat catattggaa agcatagttt aattcctcat
     6061 aagctgactc cattcttctt agaatcactt cttttgaatc agtacctctt ccttcaattc
     6121 tggattttaa ttcttccagt gacgggggaa ggataaatat gaacactccc atcggataat
     6181 tctttttaac ctgtagtgct ccttgaatat ctatttctaa tattatatca ttcccggctt
     6241 taagctgctt aaacacttct tctttgggcg taccgtaata attgccgtaa acttttgcgt
     6301 attcaagaaa ttcgtcatta tttattctat ttatgaattc ttcttcttca ataaaataat
     6361 agcttattcc gtctacctcg cctgcccttg gctttctggt cgtaacagat acggaaactt
     6421 tcaggtaact catttccaac agaagctgag cacaaattgt gcctttgcct gctcccgaag
     6481 ggcctgatat taccactaat aatccttcat ccataactca cctctacttg atattgtggt
     6541 tctcaaatct cagtgccatt gtatccggtt gaattgccga tagaattata taatcgcaat
     6601 cagtaataat tacagctctt gtttttctgc cgtaagttgc atctataagt ttattttttt
     6661 cacgggtttc ctgaataagg cgttttatcg gtgccgattc aggacttaca attgccacaa
     6721 ttctgtttgc cagcgcaata ttaccaaaac caatattaat agtatttgac atatttaact
     6781 cctattcaat attttgtact tgctctctga ttttttccaa aagattcttt atttcaataa
     6841 caacttgtat aatatctaag ctgctgactt tagagcctat ggtattaatt tctctgttag
     6901 cttcttgtaa gataaaatca agcttccttc ctattgcacc tccgctgtcc aaagcatcgg
     6961 acaattgcac catatgcgag ttaaatctta caatttcttc attaatatcg cttttatcgg
     7021 cataaattac aatttcattg taaagcctgt tttcatcaag ctcgtgagta tttcccaaca
     7081 gctctttaat cctgtcttca aatttaattt tataatcctc cacaattgta tgggagtttt
     7141 cagatatgac ttccattaat tcacgtattt ttaccaggct taggctaatg tcatccttta
     7201 actgtatacc ttctttaact ctcattgtca gcaggttttt tacagcctca ttcattgcat
     7261 tttccagaca aatacggagt tcatcatcat cggcatcatc ttcctttgca ataagaacat
     7321 cctgaaattt gattattgtg tcaagagatg ggttgccgtt aattcctaat tctgatttaa
     7381 ttttttcaat ggcatccata tactgtttag ccagggccaa attcggtaaa attactgtat
     7441 ctgattcact tatatattca atgttaatat atacatctat tctacccctt tgaatgccgt
     7501 ttttcaagat ttttcttata ttatcctcat aaatacgcaa atgtctgggg gtttttatat
     7561 ttatatcgca atacctgttg ttgatagctt ttatttcagc tgtaatactt cttttaccgt
     7621 cattgtgttc acctttgccg taaccggtca tactaaatat atttgccatt ttattctcca
     7681 attattttaa ttattttcct acttactcaa tttagagtat tatcatatac ctatctgtgt
     7741 gtcccacacc ttattacacc attataacac taaaacctta ttagtaaaca aaaatattgc
     7801 agtctaagag ctatgataga ataattcaga aaaaaccggc agccttatat taaatatttt
     7861 aaaaaattca tgacattata atgtttataa tatacaatat ggttattaac taaagaaaga
     7921 tacttactta gatattctaa tcacgagtaa caagcaggag gaaatatgtc attagacggt
     7981 atttttttaa acagtataat aaatgattta tataaaaagt taattggcgg acgagtggat
     8041 aaaatttatc agccggataa aaatgaaata gtaatatcca taagaagcaa aggagaaaac
     8101 tataaactgt taattactgc aataagcaac agccccaggc ttcatcttac aaatgtaata
     8161 aggcaaaatc catcggagcc tcccatgttt tgcatgctgt taagaaaaca cttaacaagt
     8221 gctcacataa caaatataaa acaaattaat tttgacagaa ttgtggaaat ttcatttgag
     8281 tgcaaagatg aattcggaac tgccgtgaat aaatcgctga taaccgaaat aatgggaaaa
     8341 cacagcaata ttatatttat aaacgaagat aagactataa ttgatgccat taaaagagtt
     8401 gccgaaaata taagctctgt aaggcaagtc tacccggggc tgaaatatgt tattccgcct
     8461 ggctcggata aattaaatcc gctcactgtt acaaaagatt tatttatgaa ggaaatcgag
     8521 tctacaaata acggaatata catttataat tttttatata aaaacttttt aggtcttagt
     8581 ccttttattt caagagaaat ttgttatttg accaatttaa atgaaagcac ttatttaggc
     8641 gaattaagcg aagaaaagaa agaagaaata tggaatgtgt ttagtaaaat aatgaataaa
     8701 ataaagcaaa atgatttttc ttttaatatt ttttatgatg aaaatatgaa ccaatatggc
     8761 ttttattgtt tggaggttga atatttaaaa gattatgaaa ggaaaaattt cttatcaccc
     8821 ggtgaattgc ttgatgaata ttattatgaa ttagacaaca aaaacaaaat caaccaaaga
     8881 gttgcaagcc ttattaaaaa tattaataca aaaatagaca ggaacagaaa aaaggttgaa
     8941 aagcaaagaa atgaacttct taatgccgaa aacagagata agtacaaaat ttacggagag
     9001 cttattcttg caaatcttca caaaacacct gaaaaccata agcttagggt tataaattac
     9061 tacgaccctg aacaaaagga aattacaata cccttagacc ccagatttaa tctgccacaa
     9121 aacagtcaaa aatttttcaa aagatataat aagctgaaaa aagcagaaga agaactcgaa
     9181 aaattaatcg atataacttt aaatgaaata aaatacctgg aaaatatatt attttctatt
     9241 gaagaatgtg aaacaatcga tgatttaaac gacatataca gcgagcttat ttccgaaggt
     9301 tttatgaaaa gaaaatctaa ggctgataaa tcaaacaaga caaagcctat tactgcattt
     9361 atttcttcag gcgggcatga aatactggtt ggaaaaaaca atacccaaaa tgatatgata
     9421 acatttaaaa tagctaaaaa tgaagattat tggttccatg ccaaaaatat gcccggctcc
     9481 catgttatca taagaacgaa aggcgatgaa ctaacggatg aagaattcat tgaagcagct
     9541 aaaattgcag catattacag caaggggaaa aattcaagca cggttgaagt cgactacaca
     9601 aaaaaatcaa atataaaaaa gccacctaat gcaaagcccg gctttgtaat ttacgacaca
     9661 aattattcaa tgcttgttaa gccgaatata gaagggataa aaagggacag tggggacgta
     9721 tcttgactgt cacaggtatg ggacagcaag gtacgtcccc actgtcccgc ttattcggtt
     9781 ataaccacct ggttaatttt atctgtttct tcaagccgga cgcttattct ttccgacctg
     9841 gaagtaggta cgtttttacc gatgaagtct gcacgaattg gaagttccct gtgacctctg
     9901 tcaattaata ctgctaattc cacgttatta ggtcttccct ggtccacaat ggcatcaagg
     9961 gcggctctta ctgtcctgcc tgtgtataaa acgtcgtcaa ccaataccac tgttttattc
    10021 ttaatgtcaa agtcaaaact ttttactgac aagggagccc tgtcatcctt ttcgacatca
    10081 tctctataat aagtaatatc caaaggaaaa acaggcactt tggagccttc aatttcttca
    10141 atttttttcg ccaatctgat agataagggc catcctcttg tttttattcc gattagtact
    10201 atattttcaa ctcccttatt tctctcgata atttcatgtg caatcctgaa tgttgctctt
    10261 tcaatcgctt tttcatccat tattaatgct tttattttca taaattccct cccaatatga
    10321 gatctttcgc tatcgctcag gctgacatgc aatgccctta agattttatt ttattcaata
    10381 ctttaacaaa ataatccggt aattcacttt caaattccat atacttgccg gttgtgggat
    10441 gaataaagcc caaaattttc gaatgcagga gctgtccttc caaaccaaat tcatttacat
    10501 ttccatatac atcatcacca acgacgggat gtcctatatg cttcatgtga acccttatct
    10561 gatgagtcct gccggttttt aatttcagtt tcaataaagt atatttacca aacctttcca
    10621 ctacccaata atttgtaatt gcttccttgc tgttcttatt tgttacggta tactttttcc
    10681 tgtctttttc gcttctcccc aagggtgcgt ttatttctcc ttcatctttc ttgatatttc
    10741 ctttaaccaa tgcataataa atcctgttaa ttgtgtgctc ctttaaacac ttggctaaaa
    10801 agttgtgtga tctgtcattt ttagcaacca agatcaaacc tgaagtattt ttgtctaatc
    10861 tgtgaacaat accggggcgt aaaaccccgt taatggttga caaatttttc agatgataca
    10921 taagtccgtt taccatagtg cctgaataat gccccggggc aggatgaaca accattccct
    10981 gaggtttatt tataaccgct aaatcgttat cttcataaac aatatccaag tctatgtttt
    11041 ctgccttcac atccaaaatt tcaggaggcg gtattataat ttttatagta tcgtttattt
    11101 taactttata attagcttta acttctcttt catttacggt tatatttcca tcgctgataa
    11161 gcttttggac aaagtttcgc gattcgcctg atttttcaga aatgaacacg tcaattcttt
    11221 tattttcttc gtctgaaaca aggataattt ctttttcact gtctgaaatt tccatgtctt
    11281 caaaatcatc atcatcggaa ttgtcaaaat catctatttc tttaatatcg tctaaatttt
    11341 ctatatcttt ttccgttgta ctatttaaat tatttaactt tttcattttc tcaactcgtt
    11401 tcaaacttgt taaatagaat taaaattacc ataagtattg ttccgcatac aacaaagctg
    11461 tcagctatgt taaataccgg aaaattataa aaagttccaa atcttacatc aataaaatct
    11521 attacatatc ccatgcgata tctgtcaata aaattgccta ttgctcctcc tgcaatcata
    11581 gccatggata atctggacaa ctttgaaagg tttctgtttg taaaataaac tattcctaat
    11641 ataataagca ttataatagt aataatcaaa aagaataccc tttgattctg caatatgctg
    11701 aatgcagcac ctctgttttc cgcataagtg aaacgcaaaa aatttcctat aatttttatt
    11761 gattcaccgt tttttaaaac ttctgttgcc caatactttg ataattggtc aaaaacaact
    11821 gaaaatataa ttataaatac tgctataata ctcattactt ctcccgttaa atataatgct
    11881 taatttgaat ttttagcttt ccgctttttg ttgtaccgat tattttatat attttaaacc
    11941 gtcctgccct tctgattgac aagatgtcct cctccttcaa ttctgtgctt accttgtatt
    12001 caggaatatg attgatttta acatctcccg attttattat gaaagaggcc ttttcccttg
    12061 ataattttgt aaggtgcttt acgatattgt caatgcgcat ggaggaggta ataatattca
    12121 agatttcatg ttcctgttct ttaaaattga cttggcttaa gttaattttt tctgcttcta
    12181 ttttattatg acctatttta tccaaattgt aaattatata atcagcaata tccgagtgaa
    12241 ccactacatc cccgtaatca tcacagacat agatatcccc gagcttactt ctttcaatac
    12301 ctaaggacat caatactcct aagtaatcct tatggcttaa ttttttgaat ttagacttat
    12361 tagtaatacg aattcccgaa ataaattcat tttcatctat ttcagaatat tgcgggtata
    12421 aaatgaaaac tttcctctca caattttcat atgaggggaa aatttcaaat tttatatcat
    12481 aactttttat taccggaata ctgattttta caacatatgg atttataaat tcggttattg
    12541 cggcaatata gtttttatta acatacgatg ctttatcttg tatctttttt accgtgtttt
    12601 tgatttcatc atccctgata aaatcataat atttttctaa acttttcatt tttctcccgg
    12661 tagtattgca gccatctata tggcgtatat caatctttgt aatatgttta ataatagaag
    12721 agccaatatt ggagaaaaat caaacattcc catgcctaat cccagtttat ctaaaattgc
    12781 cctgcaagga gccagtacag gctcggtcat ttgatagata aagtaggata ttcttgaagt
    12841 ttgtagggtt gggaaaaatg ataatatcac tctgaccagt attaaataat aaattattct
    12901 taatagtgat gctagagcca actgtataat ataaaacata aatgctcctt atttaccgta
    12961 ataataattc ctttcatatc tgtcgcttaa tcttccgtca atttgcacat tgttcggagc
    13021 tagtacgaat ataccgtttg atactttttg aatgcttgct tccaatgcat aaattgcacc
    13081 tttgataaat tcaaatactt gcttttggtc ctcaagttcc attccttcaa tattaagaac
    13141 tacaactttt ctgtttttaa tttcatcaac agcttttgtg caatcctcat atttttccgg
    13201 ctcacaaata aagactttca tatcagtgtt ggagtgaacc ttaatcacat tgtttctttt
    13261 tgtgttggtt cccattcttt catttttgtt tctttctgtt ccttcagaaa caagatcctc
    13321 ctcctcatag tcttcttcca agtctgtgat gccaatgaag tccttcactt tttccatcat
    13381 actcatttac tcttcctcct tatattttct ggctccaaat atagctgttc cgatacgaat
    13441 aatgttagag ccctcctcga tagcaatttc aaagtcattt gtcatgccca tggacaaata
    13501 tttcatttct gtatttctca aattcatagt tgaaatttta tcatatatat atttcatttt
    13561 tttgaaaacc catcttacat cctcagggtt ttcgcaaaac ggggcaaccg tcatcaatcc
    13621 gactactctt aaattttcaa acttctccaa actaagtata aaatcataaa cactttcttc
    13681 ttttatgcca aacttactgt cttcttctcc aatattaagt tcaacaagta catttgcaat
    13741 taaattgttt ttttctgctc ttttatttat ttcttccgcc agacttaaac tttcaacaga
    13801 atgaataagt actgttttat ctattatgta ttttacttta ttcttttgca aatgacctat
    13861 catgtgccat ttaatgtcca aatctttaag ctcgtcgtac ttttttacca gttcctgaac
    13921 cctgttttct ccaaggttat taattcctgc ctcaaaagct tctcttgctc tttctgattc
    13981 taccgtcttt gatacagcta tcactgttat atcattcggg tttcttccga cttttttaca
    14041 ggtatcttca attctcttta caatatcgtc aatattttct ctgatgctca actttgcctc
    14101 ctaatctgca atttgacctt catacacctt atcaggttca agtattactt tatcgaaaat
    14161 cttaattgta ggatatcttt tttctccgat agttattata ttcctttcat ttatacctat
    14221 atagtcaccc ggaaatacta ccgagtattc ctcattgctt cctaatattt gaacgggaaa
    14281 aaatttgatt atattgctgg catcttgtac atacacacct ttaataccgt taacttctat
    14341 cagtgattcg ttttttattt tcagcccttc atatgctgcc gtaatcagct ctatattaac
    14401 gtatctctta tcataaacct tgtagaaata gtcatcaaaa tacagtatta ataccgattc
    14461 ttcgctgccg taatttattt tttctatata accccagact atgtgctgtt ccccatctga
    14521 aagtattcta acctttatgt atttattttc ttcaaacagt cttgcttttt cattatttat
    14581 agttacaatt atataatatt caaaattcct tattatttta tagaaagctt catccttttg
    14641 aacagactct ctcttatcct tcaatgaata ctcacgtctt agggtgctgc ttggggtcat
    14701 gtccataaca ttttcaaatc tataaatacc ttctaatcca tcggttttat atgatattat
    14761 tcccgccctg tctgaataat atggaacctt atgagtagaa atactgtttg ccaagctatt
    14821 tttcatttca atcagctggc tgattgaaaa atcttcatat ttgctgccgt aaaaatcatt
    14881 attggtcatt tgttcgacta taccgtacat attctgaagg tcattgttca gtatactgat
    14941 ttgcaattcg ttttgataag cagtgaaatt ttctttagtt aaaacttgac tgctgttttc
    15001 caatttattt atggcagctt gtatttcggc aatttgtctg tttacttcaa cggaatccgc
    15061 atccgtattt atgtctgcta taagctgtcc ttgttttatt tttgtacctt cctcataata
    15121 atacaaaacg ttgccatcaa gggttgcact gtaaactttt tcatccttaa caacaattcc
    15181 ttcagcctta ataacatcct ctaatattcc gttttctgct atcataaagt ctgcaggaga
    15241 ttttatattt tgaaatacat aaatggctat aacaactaat gcaaaagaaa gaaagaaatt
    15301 ttttaaacca aaattttttc ttgccactat taatctccaa gttctatatt aagttctctt
    15361 atgtatcgag ccctcatgaa attcttcagg tttcgccttt agaatgacac tcgctgtata
    15421 atttatcatc aattgtacaa ccattataac ataatcttca tatcttgtgc aagctatatt
    15481 aagagatttt cgagaaatat tgcaataatt attaaaatta atcaatatat atgacacatt
    15541 ttttacttgc taatcttatt tcaatgcttt ttttccaatt cttccttaat accgagtgcc
    15601 acagtatctt tcggattgat ttttaacgct gtttgaatat cttctttagc tctttcaagt
    15661 tgattgttat acaaataagc tactgccctg ttgcaaaata cttcacagtt gcccgggtcc
    15721 aggcttaaca atgttgaaaa caattcagct gcttcgacat atttgcctgt gcatatttta
    15781 cacaagccca attcatttaa tgtttccttt tgagtgctgt caatttttaa tacattctca
    15841 aagtatgttt ctgcaatatc gtattctctc ttcgacctgt atgcaagacc tatgaaaaac
    15901 aacaaattcc accatccgcc aaaatccttt accaaaggca ataataagtc caaggcttcg
    15961 tctggtcttt cttttaatac aagattgtag cccttttcat aatcaacaaa tggttttaat
    16021 tgaattaatt cgtttcttat ttcttctatt cttaaatcat tatcatccaa ttcctgatgt
    16081 ttttgccaaa tgagctcggc ttttacgtat tgctgcttcc ttttataata ataacctaat
    16141 ttatagtatg ccaatgaaaa cttgtcatta tagtcaaggc atttttcata atagaaatga
    16201 gcttcttcca aaaaatatcg gcttgaatca tttccttttt catgatattc catccccatc
    16261 ttttccaaac atgaagcata aataaagcag gttttttcat tttcttcact gttaatcaaa
    16321 gctcttccgt ataccacacc gtcttctgtt ttaatgtcat tgtttatgca atatataata
    16381 taaggaatta attcgaaatt gagttcttta aacatttcct tataatcttg tatatattcg
    16441 aagtttctgt cagttcctaa tatatatatt atgccgtcaa taatattgtt cagtgttatt
    16501 ccgtccattt cattttgttt gtttatatcc tccaaaagct tttcaattct tacaggtaca
    16561 ggcagctctt tttttgcagc atatgtttta tccttatagt taacggctgc accttttttt
    16621 aaggttataa atgaaatatt atttcttttt ttttcataat actcttcaaa atatttatct
    16681 ctttccataa acaatctcct ctatgttatt aacaacataa gtatcacatc tgttgaaaat
    16741 attctccctg tccctatata caaataatac tattttccct ccgatttctg caattctgaa
    16801 atcatttata attttccctt tcattgaagg gaaatattta tttttgaatt caatgtcatt
    16861 tgccagctgc cgctttatgc ttttaaattt atcttcagtc tctctgttta aataatcagg
    16921 cagctcttca tattggttgt tgaatatata atcaaatttt aatgcttgaa caaattcatc
    16981 attctttctt ttcatatgta ctatatagtc atacaaaatt ttgtaaagct ctttcctgct
    17041 gtgagcataa ttatataaat tatatttctg ccagtaatca ctgaaagagc tgtaaaattc
    17101 aaaagctgaa tcgggaaata tatagcatgt tatatatttt aaggatttgc tgaagtattt
    17161 ttcattataa tatttatcca gcaattcttc aatatttttt aattttaata attccttcaa
    17221 acttatatat tttgtgcata tcacttcata tggagccttc aagccgtatt tgatttggtg
    17281 cttttctctg tcttcgtata ttttggttcc tttcagcacc tttaaaaaac caagctgaat
    17341 tttctctgca tcaagattat atataccgtc aaatgacctt ttaaaacttt cataatcttc
    17401 ataaggaagc cctgcaatca agtcaaggtg catatgtaca tttttgtttt ttcctatttc
    17461 cttaataacg cggcataatt tatccttgtc catatgcctg ttgatttcac gcaaagtatc
    17521 ttcattcaag gattgaacac caatttcaaa ctgaaacata ttaaccggca ttttcgctat
    17581 aaagcttaaa aactcatcat ttattatgtc ggcagttatt tcaaaatgta ttgtcatatt
    17641 atttctgtta ttttctacaa tatactgcat tatctccatg gagcgcctga aatctgcatt
    17701 gaatgttctg tccacaaact taatctgcct tgcctttgta tttaaaagca tctttaaatc
    17761 ttttttaact ctatccatcg aaaaaaatct cattttttta tcgatggatg acatgcagaa
    17821 gctgcagcta aaaggacatc cccttgaaga ttcatagtat attattttat ttgcaaattt
    17881 atcatcaacc tcataggggt aattaattat gtcgagatta tctatcggct ctctgccttc
    17941 gtttatgatt acatcattgt tttctttata tgccagaccc ctgattttat aaagattaga
    18001 atttgtattt tgaatttctt caagaaattc tttaaatgtt tcttcccctt ccccgaagat
    18061 tacataatcg atgaaggggt tgttttccat taattctttt atttcaaaag aagcctcggg
    18121 acctccgtac aatatcttgg ctttattatt tgattccttt attatgtatg aaatatcctt
    18181 tatataatca atattccata tatagcagga aaaacaaatt aaatctgcat cttcagccaa
    18241 aatttcatcc agtatgtatt cgattttttg atttattgta aattctttta ttacaatatc
    18301 aaaacccctg caatactttt ttaaatatcg cactgctaaa ttagcatgaa taaattttga
    18361 atctaaggtt accaacaata ttttcatatt ctaatcctca tcagccccaa tttattccta
    18421 acaattgctt ctttttcggt tctttttata accttgcatc tccctaacag gggatggctg
    18481 aatgtcgaat actttaagca ttcctttatt tccggaattt tatccggtat tattaatttt
    18541 tcaggactta ttaaaccctt cctgagtttg tacaaatata cagaaaagct gtctattata
    18601 tccgagttat tttcggttaa atgttccggg ctcttttcaa atatgtaatt taagaccttg
    18661 aatccgttga taaaattatt atcgaacagt ataggcttag caatcatttt tattctttct
    18721 ttttcaaaat cgattatata atttttatct gcatttattt gattttttat taaaaattct
    18781 tttaccgaaa aatttataag cctttccctt gctgctgcct caatactgtc aatatcagag
    18841 cctgtcataa aatcaaaaac tgcagatgaa ataagcaaag cattcaatat ggcattttta
    18901 tttattgtgt agaataaaac cacttttata agttcatccg ttaaattttc ccacacagtg
    18961 tttgacaagg caagtggaat gattttatac tctaaaagat tgtcatctaa atttgacgcc
    19021 tctttatttc gcagccagta atcaattgtt tcatcattac cattcatata agaggaaaat
    19081 aattcaattt cctttttata tctatcttta tcaaaaaggc atccgatttt atcatcatag
    19141 ggtatgagac attttaccgt aattaaaaag agctgaaagt caattttttg tatgtcgtcg
    19201 aaacagaaat ttagcatctt gtttatttta tattgcatat attctcctaa atcgcattgg
    19261 aatgccgcaa atttatggca ttccttgcgt ttgacccctt atccctttat gttttgctat
    19321 tacatcagca atggagtcag gtcataaaaa agaattcgca gtattaactg cgattccatt
    19381 atacaatcaa ttattgaaat tatcaaatgc tattaatcaa tactttaaat tttcaaattc
    19441 ttctttataa atggatgcaa attgtaaaac cttagtagag taatcactaa cacctctccc
    19501 atcacatgtt gtcaaatagt ttttgagtcc ttgctgacct ctgttgtaag cagtgagagt
    19561 tttatgcatg tcattatcgt aaactttatt aagatatgaa agctgtgcaa ttgttagttt
    19621 taaattaaat accgggtcta aaagcatttc attattatat gaatatccta aattcatagc
    19681 aataatttgg gcagtattct ccatcagctg acccaaaccc aattctcctc cgacaccctt
    19741 tgccttggga ttaaaatcac tttctctttt tattaaaccc aatacaataa aggggtctat
    19801 gtatttttct ttgctttctt taataagaaa tctacagtgc tcgtaatcta atcccgtcag
    19861 ttcttctgta taccggatca attctttttc gcgaataaat gaatccctgt aatcatgaat
    19921 aattgatttt attattttat cctgtgaaaa tttgtaataa ttttgattat cctctgctat
    19981 tgcagtcatg cttgtattta tcatcataaa gtaagaaaat atacatagaa atattaaaaa
    20041 agtcaaataa tttacccttt tcatttcgta cctccttgtc caatattaat tataatgtaa
    20101 atttaaatta tgctcaatgt tgaaatttga aatgtattgt tgaataggga gttaatttga
    20161 cgttttagta aaaaaaatcg gtgccaggca ccaaaattgg tgcctggcac cgaagtttac
    20221 gtagttacat cttctccggt gcttccattc ccagaag
//
LOCUS       DAOPUE010000029        19528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2818_length_19528_cov_10.745956, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000029 DAOPUE010000000
VERSION     DAOPUE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19528
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_19528_cov_10.745956"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKH42_04235"
     CDS             complement(<1..595)
                     /locus_tag="PKH42_04235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOA19674.1"
                     /translation="MVIKKDFYYDSADKKSKIHAVKWVPDAEIRGVLQIAHGMLEHID
                     RYDCFARYMAENGILTAGNDHLGHGDSVLVEEDRGFFGEGDGNKLVTKDMRKLREILK
                     EEYEGLPYFILGHSMGSFLVRQYISIYNDLNGALLVGTAYQPYLSVKAGLIISKLMAV
                     FKGWRYRSKFINKLTIGGNNKYFEPAKTKSDWLSRDEE"
     gene            complement(622..1173)
                     /locus_tag="PKH42_04240"
     CDS             complement(622..1173)
                     /locus_tag="PKH42_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOA19675.1"
                     /translation="MIKEFEGIKPIIDEKTYIADGAQIIGKVTIKEYSSIWFNTVVRG
                     DVNYIEIGRYTNIQDNSVVHVADDYPAIIGDYVTIGHNATVHACKIEDHCLIGMGAII
                     MDGAVIGRGSIIAAGAVVTNNQKIPPFSVAAGIPAKVIKTVPEEDIIKIHSQALKYKH
                     VWSIRYGIMPDCGGESYDGGEII"
     gene            complement(1243..1656)
                     /gene="ndk"
                     /locus_tag="PKH42_04245"
     CDS             complement(1243..1656)
                     /gene="ndk"
                     /locus_tag="PKH42_04245"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOA19676.1"
                     /translation="MSDFLGKERTLVLIKPDAIERKLMGEIISVYEKNFNIIDMKMIK
                     PSVEIAEEHYYEHRDKPFFNELISYIIRGNVCALVIEGKNVIDRVRKINGATDPKNAE
                     PDSIRGKFALSKQENSVHGSDSKESAEREIKIWFG"
     gene            complement(1654..2049)
                     /locus_tag="PKH42_04250"
                     /pseudo
     CDS             complement(1654..2049)
                     /locus_tag="PKH42_04250"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
     gene            complement(2353..3150)
                     /locus_tag="PKH42_04255"
     CDS             complement(2353..3150)
                     /locus_tag="PKH42_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOA19677.1"
                     /translation="MANKYHDFSPKEVRQLIREEKIQEPTSGMCGGYAQANLVILPKK
                     YADDFKLFAEYNPKPCPILEITEPGSYTTTKTADKANIITDIPKYFIYKNGVRVDEVL
                     NAEKYWGDDFVCFLIGCSFSFEEAMIREGIEIRHISMGRNVPMYKTNIEAKPAGIFKG
                     PYVVSMRPMKPEDVDKAYEITGKFPHVHGAPIHHGDPAEIGIKDINKPDYGEAVIIKE
                     GEVPVFWACGVTPQAAIENAKPEIVITHAPGHMFITDILNEDIESIF"
     gene            complement(3214..3627)
                     /locus_tag="PKH42_04260"
     CDS             complement(3214..3627)
                     /locus_tag="PKH42_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19678.1"
                     /translation="MDIINHKITEGLSITYQKQTNASDAYVSNTSSIENLVSTPALLT
                     MIVEASCKMLDSLIPQQCLTVGNRLEISHINPTLVGDTITLILTVEKVDGNKILLDIV
                     GADSNGRICLGKYHKVIVERNKLMENAYKRSKDWQ"
     gene            complement(3664..4731)
                     /locus_tag="PKH42_04265"
     CDS             complement(3664..4731)
                     /locus_tag="PKH42_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOA19679.1"
                     /translation="MSSVKVKKGGFLTTVQDLGRIGYQKFGMPVAGVMDNFSYRVANF
                     LVGNNEGEAVLEATLLGPELEFGCDMSFAVTGANMKPMLNGKEISMWESYKVTKGDVL
                     SLGVVTSGLRAYIAFSGSIEVPLLNNSKSTYLKTGIGGFKGRKLMNDDILEIKVSDKI
                     VYGKYMDPKYIPEFKRKGNVRVVLGPQDDYFTEEGINTFLSPEGYKITKEADRMGYRL
                     DGKTIKHIDKADIISDGALFGSVQVPANGLPIILMADRQTAGGYTKIATVISTDLPML
                     AQMGEGCQLVFEKITLEEAHKLYKEYEEKLSEIEKQIANSQRVIEAPVEKAAYVNIGP
                     VRRMNIVINGVNYVVDVQEIQ"
     gene            complement(4724..5470)
                     /gene="pxpB"
                     /locus_tag="PKH42_04270"
     CDS             complement(4724..5470)
                     /gene="pxpB"
                     /locus_tag="PKH42_04270"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOA19680.1"
                     /translation="MYEKIKYLSAGDKAVVMEFGNEISKEINAKIRNVVKLIDEAKID
                     GIVELLPTYRSLMIMYDPLKIEYSDLISTLDSMSSKQVESSDEKIRIVEFPTVYGGEY
                     GPDINFVAEHNNITVDEVIKIHTGTDYLVYMMGFTPGFTYLGGMSNKIATPRLSSPRT
                     KIPAGSVGIAGAQTGMYPSETPGGWQLIGRTPLKLYDPDKEPPVMLSAGDYVRYVSVS
                     EEEYLEIKKQVEEGTYEVKVIISEGGDLRE"
     gene            complement(5498..6694)
                     /locus_tag="PKH42_04275"
     CDS             complement(5498..6694)
                     /locus_tag="PKH42_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOA19681.1"
                     /translation="MSERDKSQDKRILIGAAFLMATSAIGPGFLTQTTTFTAQLGPTF
                     GFVILVSVLFSLIAQVNIWRVICISKARGQDIANKVFPGLGYVVAILVAMGGLAFNIG
                     NVGGAGLGLQVLFGLDLNVAIAISGIIGIIIFSVKQVGNAMDKFATFCGALMIILILY
                     VSISSKPPVGQAIKETFVPSSFPYLQILTLIGGTVGGYISFSGGHRLIDAGIVGEENF
                     DRVNQSAIMGMGVATIVRILLFLAVLGVVSKGATLDASNPAADAFKIAAGTLGYKIFG
                     MVMAAAALTSVVGAAFTSVSFLRTLIKPIGDHVNITTIAFIVASTAIQIFVGSPAKVL
                     VIVGSLNGLILPLTLGTILVASKRKDIVGEYKHAQWLFILGWIVVAVTAVLGLRSLTG
                     IAALWK"
     gene            complement(6793..7560)
                     /locus_tag="PKH42_04280"
     CDS             complement(6793..7560)
                     /locus_tag="PKH42_04280"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOA19682.1"
                     /translation="MYKVDLNSDVGESFGNYKLGLDSEVLKYVSSANIACGWHAGDAM
                     VMQKTVDFAIKEDTCIGAHPGFPDLMGFGRREMKVTKEEAKNYVKYQIGALWAFAKSC
                     GTQLQHVKAHGGLYNMAAKDPELAMAIAEAIYEVDKDLILMGLANSEMIKAGKAVGLK
                     TANEVFADRAYNTDGTLVSRKLEGSMITDETLALTRVVRMVKEGKVQAITGEDIDIKA
                     DSICVHGDNPKAVEFVKTIRKALEDEGIQIMPLKDFI"
     gene            complement(7735..9144)
                     /locus_tag="PKH42_04285"
     CDS             complement(7735..9144)
                     /locus_tag="PKH42_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOA19683.1"
                     /translation="MKIRILIVDDEETIRLSLEEALKDLGYSISTAATGHIALEKVKE
                     YKPQIVFLDMRLAGENGLDILPKIKKIDKKVEVIIMTAYGDINTAVKAIKLGAFDYIN
                     KPFDLIDVEILIKRITENLKLQNKIYLLEKEKEKELNKKIIIGNHPLMNEVMNRIDIL
                     SKNDNVTVLIRGETGTGKDLVASYIHNHSIRRGSAMLKLNCGAIPQQLIESELFGFEK
                     NAFTGANNRKKGLLEIADGSTVFLDEIAEMPMEVQPKLLRFLDERKFKRVGGLEDIEV
                     DIRIIAATNKNLEKAIEDKEFREDLYYRLNVVPVYLPLLRERGDDILVLANNFLYEYN
                     CKFNKKIKGFTDRAKEALLLYSWKGNVRELKNVIERIVILVENEYIDIGDLPLEIRSA
                     LGGGNEFTKPSQDEMSSKLLSGGFSLENEVAEMEAEYIKLALKYCSNNHTKAAEMLGI
                     SRFAFKRRIEKYFYADNNA"
     gene            complement(9134..10891)
                     /locus_tag="PKH42_04290"
     CDS             complement(9134..10891)
                     /locus_tag="PKH42_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19684.1"
                     /translation="MRNSIEKKILIPFIIILMVSILIIVIVFVVNGYNLLLDNHINIS
                     YRNLEEMILTIEKVNDDIKDEIGAKDFIINFYKDLGKNNLIIFSDDDDEILLNTVLNN
                     NFIEEFMNEELNKGPESISVGNYIFTYQKYDKWNWVIAYGLNRDNLLIEVMESQQYII
                     LVALIALVFSMHASIFITHNISKPIKMLAEHCNKIVADGIYKDRIEIDRNDEIGTLAD
                     AFNNMLDTVQNNTDRLIEATKFNEDILRNVSAGIVTADQHGSVISINQTAENLLIPAA
                     EKIGLVKECLHEQIMETLNTKMKINKILAFDDEHGNKKYIDVTTSLLIGGDGSISGAI
                     CNFNDITERKRIENSMSTLDRLTTIGQFASGIAHEVRNPLAGMKTSIQVLKNRLCKED
                     NDMIASNKKLLNGVLFEIDRLNNLITDLLNFAKPSFPNIEKTDLMGVLDKSLNLIGET
                     AKKNNIKINIDNNCKNTVAYVDKAQTEQIFINIINNAFFAMDKGGTLNISFNNCSQKE
                     KFIEVIFHDNGCGIQSQDLAKVFNPFFTTNPQGTGLGLSVVYELVMGNRGEIDIQSAV
                     NEGTKVIIRFPINGGSNEN"
     gene            complement(11137..12411)
                     /gene="serS"
                     /locus_tag="PKH42_04295"
     CDS             complement(11137..12411)
                     /gene="serS"
                     /locus_tag="PKH42_04295"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOA19685.1"
                     /translation="MLDIKRIRNNPNEVEELLKRRNSELSLDKVLALDKARREKLVEV
                     ENMKAEQNKVSKQVPLLKKEGKDVTEILQKMKELAEKVKVLDGQVKEIDEKIEEELLN
                     IPNTPNPDITVGASDADNKEIRRWGEPRKFDFELKAHWDLGVDLDILDFERATKITGT
                     RFSMFKGEGARLERAIIAYMLDKHTLDHGFTEISPPFMVNRDSMIGTGQLPKFEEDMF
                     KLNQREYYLIPTAEVPVTNIYRNEVLDEKDLPMYMTAYTPCFRAEAGSAGRDTRGLIR
                     NHQFDKVEMVMYAKPEDSYNQLEILTNFAEEILKGLGLPYRVVELCTGDIGFSSAKTY
                     DLEVWMPSYNRYVEISSCSNFEDFQARRANIKFRPAEKGKLEYIHTLNGSGLAVGRTF
                     AAILENYQNEDGTVTIPNVLRDYMRGLEKITK"
     misc_binding    complement(12444..12704)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12743..13297)
                     /locus_tag="PKH42_04300"
     CDS             complement(12743..13297)
                     /locus_tag="PKH42_04300"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOA19686.1"
                     /translation="MIGKKIRNIRKNKNMTIVELSEKINVTSGYISQIERDLISPSLA
                     VLKRLSDALDVPLSVLFSEESSNDVITIPKEERTKVKLNNINVELEFITPILKNSHKQ
                     SEFEAFLFKMKPKTWISNHSITHESKEYIFVTQGEIECHVGEKIYTISKGDSIIVPEN
                     NNHMYYNKNDDVSEALCIISPSIH"
     gene            13560..15794
                     /locus_tag="PKH42_04305"
     CDS             13560..15794
                     /locus_tag="PKH42_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA19687.1"
                     /translation="MADNRVYLEEIKYLDEIINLLKNKLEYEKNQLENKKSDLIESRR
                     EMWENTTHSSADFDKLTDLNQYLSALQAQTFTYAELAKRILKHEKMLDRPYFARIDFT
                     EEGYDDIEKIYIGLFNLMDDETHEIKIYDWRAPISGLYYRNEVGPVEYKAPAGLIKGY
                     VSLKRQFKITKGKMEYFIDSNINILDEMLMVALSKNMTSKMKTIVETIQKQQDLIIRD
                     LYNDLLVVQGVAGSGKSSVALHRIAYLMYQGLNLKLRANNVIVISPNPLFSKYISNVL
                     PELGEDSINEYTFENIFHKLFGNKLSMKSKSENFENIITAETEEQRNFLRSYDDFKGS
                     FVFSKIIDRFIEYYEHKLINFEDVYYNGKIIEKRELAKAFLLSGKLDMPTAKKLKIIE
                     SRILEKVRENRIERRNKIEKAVNKLNNHEFEIMSFTRLLSAKETLALTERLKKFTEFD
                     VFELYKKLFADIILFKKISKGLELPENIDEIISYTNTTLSDSYNIPYADGIALTYMKI
                     KAEGCDLFSDIKQVVVDEAQDYYPIHYLILKNLYKYARFTIVGDINQSIEKSNDLSLY
                     DDIISIFNFEKSSKVFLNKSYRSSYEIGKFSAKLSGDENYAEFFKRNEEEPVIFKFKD
                     INNLRDEIIKTIEKYKTEGFSSIAIICKDRKEASDLYFKLANKIKIKLIDYLDYDSVL
                     EGTLILPVYLAKGLEFDAVIVYEANDKHYNNEFDKKLLYVACTRALHRLSLFYTGNLT
                     KFLD"
     gene            complement(15846..15916)
                     /locus_tag="PKH42_04310"
     tRNA            complement(15846..15916)
                     /locus_tag="PKH42_04310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15882..15884),aa:Gly,seq:ccc)
     gene            complement(15923..15995)
                     /locus_tag="PKH42_04315"
     tRNA            complement(15923..15995)
                     /locus_tag="PKH42_04315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15960..15962),aa:Ala,seq:ggc)
     gene            complement(16086..16158)
                     /locus_tag="PKH42_04320"
     tRNA            complement(16086..16158)
                     /locus_tag="PKH42_04320"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16123..16125),aa:Thr,seq:cgt)
     gene            16297..16563
                     /locus_tag="PKH42_04325"
     CDS             16297..16563
                     /locus_tag="PKH42_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19688.1"
                     /translation="MDDYNQYYDTMNDYMYYDDINKDVYSSQYDMYDRRFDNRFDNKF
                     DRRFDRRYDRFFYDPMRYYRFPFCDRYGRCENPIWWLFWPFFFQ"
     gene            complement(16595..17155)
                     /gene="pyrE"
                     /locus_tag="PKH42_04330"
     CDS             complement(16595..17155)
                     /gene="pyrE"
                     /locus_tag="PKH42_04330"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOA19689.1"
                     /translation="MIDVIEILKECGALLEGHFLLSSGKHSNKYCQCAKLLQYPDKAE
                     KVISLVYEKVKSLNLDVIVGPAMGGITAAYELGRQLGIKTIFTERENNVMTLRRGFEI
                     QPGDRVLIMEDVVTTGKSSMETAKVLEEAGGIVVGIGCIVDRKVCEIQFPVYSSIELF
                     FETYDDKNCPLCEMGSKPIKPGSRKF"
     gene            complement(17175..18077)
                     /locus_tag="PKH42_04335"
     CDS             complement(17175..18077)
                     /locus_tag="PKH42_04335"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOA19690.1"
                     /translation="MANLKTEAMDLKFKNPIIAASGTFGFGEEFSQIYDLSILGGICS
                     KGLTLTPRSGNKGIRLWETPMGLMNSIGLENPGVDKFITEELPRMKTYNTIIFANLGG
                     HCEEDYLEGIYKLNDTNVDAVELNISCPNVKSGGIAFGVKSSVAYDIVSRVRKICKKP
                     LMVKLSPNAENIPDMAVACESAGADAISLINTIQAMAIDIKNRKAVFENVYAGLSGPC
                     VKPIALRMVHEAAKMVNIPICGLGGIMNAIDVIEFIMAGATLVQIGTANFIKPDIAVD
                     ILNDLEEFMDNENIKSLEEIRGII"
     gene            complement(18087..18815)
                     /locus_tag="PKH42_04340"
     CDS             complement(18087..18815)
                     /locus_tag="PKH42_04340"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOA19691.1"
                     /translation="MKVLVTAVAENEQISKKIYRLSVKFDSEIKPGQFFMLKTTDNAF
                     LLPRPISVNDVNENLVSFLYRLEGQGTKKISSLRKNDEIQIFGPLGKGFEPDKLLGRV
                     AVIGGGIGVAPLLYLCKVLGKKADVYLGYRDKENMYIIDEFKSSARRCIVVTEDGSFG
                     EKGYVTDYISYDDYNVVVTCGPEIMMSKIATICRDKKIKCYVSLERRMACGMGACLGC
                     TVETKTGNKRACKEGPVFDSEELI"
     gene            complement(18838..19263)
                     /locus_tag="PKH42_04345"
     CDS             complement(18838..19263)
                     /locus_tag="PKH42_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOA19692.1"
                     /translation="MLNIDSVEKGLVIDHIKAGKAMEIYHYLNLDKMDCSVAIIKNAK
                     SKKYGKKDIIKVENNLDMDLKVLGFIDPNITINVIDNSVIIDKINLELPQEVENVVKC
                     KNPRCITSIEQEIVHKFRLADREKGIYRCVYCDTAYDKK"
     gene            complement(19263..>19528)
                     /locus_tag="PKH42_04350"
     CDS             complement(19263..>19528)
                     /locus_tag="PKH42_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009662583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOA19693.1"
                     /translation="LDVLYMTRVQKERFFNEADYIRLKDSYIVDNKKMSNAKKDMILM
                     HPLPRVNEISVEVDNDPRAAYFIQAKCGMYVRMALILKLLGVE"
BASE COUNT         5880 a         3873 c         2797 g         6978 t
ORIGIN      
        1 cttcttcgtc cctgctaagc caatccgact tggttttagc aggctcaaaa tatttattgt
       61 tgcctccgat tgtcagctta tttataaact tgcttctgta tctccatcct ttaaaaactg
      121 ccattaactt agaaattatc agacctgctt ttactgataa atatggctga taagcggttc
      181 ctacgagtaa ggcaccattc aaatcattat atattgaaat atactgtctt accagaaatg
      241 aacccatact gtggcctaat ataaaatagg gcaagccttc atattcttcc ttcaatattt
      301 ctcttaattt tcgcatatct ttggttacta acttatttcc gtcgccttca ccgaaaaaac
      361 cgcggtcttc ttcaacaagc actgaatctc catgtccaag atggtcattt cctgcagtca
      421 gtattccgtt ttcggccata tagcgtgcaa agcagtcgta cctgtcaata tgctcgagca
      481 tcccgtgagc aatttgaagt acacccctta tttcagcatc aggcacccat ttaaccgcat
      541 gaatctttga ttttttatcg gctgaatcat aataaaagtc tttcttgatt accataatta
      601 ctccataaat ttaatatata actatataat ttccccgcca tcatatgact ctccgccgca
      661 atcaggcata ataccatacc tgattgacca aacatgttta tattttaatg cttgggaatg
      721 tattttaatt atatcttctt caggtacagt cttaattact ttggcaggta ttcctgcggc
      781 aacagaaaaa ggaggtattt tttggttgtt ggtaactacc gcacctgcag ctataatgct
      841 tccccttcct ataacagcac cgtccataat aattgcaccc ataccgatta agcagtggtc
      901 ttcaatttta caggcatgaa cagttgcatt gtgcccgatt gttacgtaat ctccaattat
      961 tgccggataa tcgtcggcaa catgtacaac gctgttatct tgaatatttg tatacctgcc
     1021 tatttctata tagtttacat ctcctcttac aaccgtatta aaccaaatac ttgaatattc
     1081 ttttattgtt accttgccta ttatttgagc accatcggca atgtatgttt tttcatctat
     1141 tattggctta ataccttcaa attctttaat cattatttcc tcccatacag gacgttcttc
     1201 aattctatcc aaagaattac cttcatagat gtcccttttt tattatccga accaaatctt
     1261 tatttctctt tctgcacttt ccttagaatc tgagccatgt actgaatttt cctgttttga
     1321 taaagcaaat tttcccctga ttgaatcagg ctcagcattt tttgggtctg ttgctccgtt
     1381 aatttttcta accctatcaa ttacattttt accttcaata accaatgcgc acacatttcc
     1441 cctgataata taagaaataa gttcattgaa aaatggtttg tccctgtgct cataataatg
     1501 ttcttctgca atttcaacag atggcttaat cattttcata tcaattatat taaaattctt
     1561 ttcataaaca gaaattattt cacccataag ttttctttca attgcatccg gttttataag
     1621 caccaatgtt ctttcttttc ctaaaaagtc tgatattttt gaaagcagct catcaatatt
     1681 attaacatca agcgacagtg tttcaatagg acacattcct gattgttcac gattttttat
     1741 gtatgaaacc aatttttgat attcaaacac aatagaagtg tttttcaaaa tatccacagc
     1801 attttgagaa attaatttag tgcttagttc tttgatatca gcatgtttat aaatcattgc
     1861 agccgcctta ccaacaactt tatcagccgc actagagcct ttaagttcgt ctttgagctc
     1921 attaaaagct tcacaaacag gtttaattcc tttattttca cttgtaaaaa ttacttttcc
     1981 atccttgaca attactattg ttttattttc attattcaat atttgttttg ctaattcaat
     2041 gtctttcata tatactccaa ttcttcgctg tgctcaggat gacaaacata aggtttgtca
     2101 agcctatgca atggctaaag tttttgcttc attgattgta ttcattatag tatagtacag
     2161 taattggtga tttgcaataa taaaaaccgg cataaaccct gagttaagaa tgctaacaaa
     2221 taaaatccaa aatatttggt ttaaatattt tggatttatt tgtattgtcc tgctatcctt
     2281 gaggcaattg acatatatta tcacgactta ttaaagccgg gtttcatatt cgaataatta
     2341 attgtttgac ctctagaata tgctttcgat atcttcatta agaatatcgg ttataaacat
     2401 atgacccggt gcatgagtta taactatttc aggtttagca ttttcaattg cagcttgcgg
     2461 tgtaactcca catgcccaaa ataccggtac ttctccttct tttattataa ctgcttcgcc
     2521 ataatcaggt ttattgatat ccttaattcc tatttccgca gggtcgccgt gatgtatcgg
     2581 agcaccatga acatgcggga atttccctgt tatttcatat gctttatcta catcctcggg
     2641 tttcataggt ctcatgctta caacatatgg tcctttgaat attccggctg gtttagcctc
     2701 tatatttgtt ttatacatag gaacattacg acccattgaa atatgtctga tttcaattcc
     2761 ttctcttatc attgcttctt caaatgagaa actgcagccg ataaggaaac aaacgaaatc
     2821 gtctccccaa tatttttctg catttaacac ttcatctact cttactccgt ttttatatat
     2881 aaaatatttt ggaatatcgg taatgatgtt tgccttatct gcagtctttg ttgtagtata
     2941 acttcccggt tcggttattt caagtatagg acatggttta ggattatact ccgcaaataa
     3001 tttaaaatca tctgcgtatt ttttaggtaa tattaccaga tttgcttgag catatccacc
     3061 gcacataccg gatgtcggtt cttgaatttt ttcttctctt attaattgtc ttacttcttt
     3121 tggagaaaaa tcatgatatt tattagccat ttatcttatc ctttctataa atattgatag
     3181 tattatttaa tctatcttgc acattttatt gtatcactgc caatctttcg accttttata
     3241 ggcattttcc atcaacttgt ttctctccac aataacttta tgatattttc ctaagcaaat
     3301 ccttccgttc gaatccgcac ctacgatatc aagaagtatc ttattgccat ctactttttc
     3361 taccgtaagt atcaatgtta ttgtatcacc gaccaatgtt ggatttatat gagaaatttc
     3421 taatcggttt ccaacagtta aacattgctg tggaattaaa ctatcaagca ttttgcaaga
     3481 agcttcaacg atcatagtca aaagagccgg agttgaaaca aggttttcta ttgaagatgt
     3541 atttgaaaca tatgcatcag aagcattagt ctgcttttga tatgttattg aaagaccttc
     3601 ggtaatttta tggtttataa tgtccacctc catatattca tttgcttaat cgtatcttag
     3661 aattcattga atctcttgaa catctaccac gtaatttact ccgtttatca ctatattcat
     3721 tcttcttacg ggtccaatgt tcacatatgc cgctttttca acaggtgctt caattactct
     3781 ttgtgaattt gctatttgtt tttcaatctc agatagtttt tcttcatact ctttatacaa
     3841 tttatgtgct tcttccaatg ttatcttctc aaatactaat tgacagcctt cgcccatttg
     3901 agcaagcatt ggtaagtcgg ttgatataac cgtagcaatt tttgtataac cgcctgctgt
     3961 ttgtctgtct gccatcagta taataggaag tccgttagca ggcacctgca ctgagccgaa
     4021 taaagcaccg tcggaaatta tgtcggcttt atctatatgc ttaatagttt ttccgtcaag
     4081 cctgtatccc attctatctg cttctttggt tattttatat ccttcgggac ttaaaaaagt
     4141 atttatacct tcttccgtaa aataatcgtc ctggggacct aataccactc ttacattacc
     4201 ttttcttttg aattccggta tatatttagg atccatatat tttccgtaca ctattttgtc
     4261 ggatacttta atttccaaga tatcatcatt cattaatttt cggcctttaa aaccgccgat
     4321 tcctgttttt aagtaagttg atttgctgtt gtttaagaga ggtacttcaa ttgaaccgct
     4381 gaaagctata taggctctga gaccgcttgt aactactcca agagataata cgtcaccctt
     4441 tgtaacttta tagctttccc acattgaaat ttctttgccg tttagcatag gtttcatgtt
     4501 tgcgcctgtt acagcaaatg acatatcgca gccaaattcc aattccggtc ctaataaagt
     4561 agcttccaat acagcctcgc cctcattatt tccaaccaag aaattagcaa ctctatatga
     4621 aaaattgtcc ataacaccag caacaggcat tccgaatttt tggtaaccaa tacgtcctaa
     4681 atcttgtact gtagttaaaa acccgccttt cttaactttt acgctactca cgcaggtcac
     4741 ctccttcgga aattatcact tttacttcat atgtaccttc ttctacttgt tttttaattt
     4801 ccaagtattc ttcttcactt actgatacat atcttacata atcgcctgca ctaagcataa
     4861 ccggaggctc cttgtcagga tcatataact ttaacggagt cctgccaatc agctgccacc
     4921 cgccaggtgt ttccgacgga tacattcctg tttgtgcccc tgcaattcct acagagcctg
     4981 caggaatttt tgttcttggt gaggacagtc taggtgtggc tattttattg ctcatacctc
     5041 ctaaatatgt gaatccggga gtaaatccca tcatataaac cagataatcc gtacctgtat
     5101 gaattttaat aacctcatct acagtaatat tattatgttc tgcaacaaag ttaatatcag
     5161 gtccatattc tccgccatac acagtaggaa actccacaat tcgaattttt tcatctgaac
     5221 tttccacttg cttgctactc atagaatcaa gagttgaaat tagatcagaa tactctatct
     5281 ttaacgggtc atacataatc atcaaagacc tgtatgtagg caacaattca actattccat
     5341 caattttagc ttcatctatc agttttacga catttctgat tttagcattg atttctttgg
     5401 aaatttcatt gccaaattcc attactacag ctttatctcc tgcagaaagg tattttatct
     5461 tttcatacat atcattattc ctttcatcaa ttcttgttta tttccacaat gctgctatac
     5521 cggtaagtga tcttaaacct aagacagctg ttacagcaac aactatccat cctaatataa
     5581 ataaccactg tgcatgtttg tattcaccca caatgtcttt tctcttagaa gctacaagta
     5641 ttgttcctaa tgtaagcggt aaaataagtc cgtttaacga gcctacaata accaatactt
     5701 ttgcaggact gcctacaaat atttgtattg cggttgatgc tactataaat gctatggtag
     5761 ttatattaac atggtcgcct attggtttaa ttaatgttct taagaaagaa accgatgtga
     5821 aagctgctcc aacaacagaa gttaatgcag cggcagccat aaccattccg aaaattttat
     5881 agcctaaggt acctgctgct attttgaatg catctgctgc agggttactt gcatctaatg
     5941 tagctccttt tgaaacaaca cctaatacag ctaagaataa tagtattctt actatagttg
     6001 caactcccat acccataata gccgattgat taactctgtc aaaattttct tctcctacga
     6061 ttcccgcatc aataagtctg tgtccgcctg agaaagaaat gtatcctcct actgttcctc
     6121 ctatcaaggt caatatttgc agataaggga atgaactggg aacgaatgtt tcttttattg
     6181 cttgaccaac cggtggcttt gatgaaattg aaacatatag tatcaaaata atcattaatg
     6241 ctccgcagaa tgttgcaaat ttgtccattg catttccaac ttgcttaacg gagaatatga
     6301 ttataccgat aattcccgaa attgcaatag ctacatttaa gtctaatcca aacaaaactt
     6361 gtaatcccaa tccggctccg ccaacgttac cgatgttgaa agccaaacca cccattgcaa
     6421 ccaaaattgc cactacatat cctaggccgg ggaatacttt atttgcaata tcttgacctc
     6481 ttgcttttga tatacaaata actctccaga tattaacctg agcaattaaa gaaaaaagaa
     6541 ctgatactaa aataacgaat ccgaatgtcg gtcctaattg tgccgtaaaa gttgtagttt
     6601 gtgttaaaaa tccaggtcct attgcagatg ttgccatcaa aaatgcagca cctataagga
     6661 ttcttttgtc ctgtgattta tctctttctg acataatatt aatctccttt attattatta
     6721 ttagtcggtt atattcttaa ccgctattat gttacttgaa tttgtctttt gtaagtagat
     6781 atacctttgt tgttaaataa aatcttttaa aggcattatc tggatacctt catcttccaa
     6841 agcttttctt attgttttta caaattcgac agctttagga ttatcaccat gtacacaaat
     6901 tgagtctgct ttaatatcaa tatcttctcc ggtgatagcc tgaactttgc cttctttaac
     6961 cattctaaca acccttgtaa gtgcaagagt ttcatcagtt atcatagagc cttccagttt
     7021 ccttgaaacc agagtcccat cggtattata agccctgtcc gcaaatactt catttgctgt
     7081 cttaagacct actgccttgc cggcctttat catttcgctg tttgcaagac ccatcaaaat
     7141 taagtctttg tcaacttcat aaattgcctc tgcaattgcc atagccaatt caggatcttt
     7201 tgcagccatg ttgtataaac cgccatgagc tttaacatgc tgcagctgtg ttccacagct
     7261 tttcgcaaat gcccacaaag cacctatttg atatttcaca tagttctttg cttcctcttt
     7321 tgttaccttc atttctcttc tgccaaatcc cattaaatca gggaatccgg gatgtgcgcc
     7381 aatgcaagtg tcttctttaa tagcaaaatc aactgttttt tgcattacca tcgcatctcc
     7441 tgcatgccat ccgcatgcaa tatttgctga cgaaacgtat tttaggactt cagaatccaa
     7501 gcctagttta taatttccaa aactttcgcc aacatcactg tttaaatcta ctttgtacaa
     7561 aattaatcac ctccaaataa atattatgca attaatatgc caagattatc tttaataata
     7621 atctccatat ttcaacaaaa aataaagagt aaataaatat ttgcgtgcga atatgcacgt
     7681 tttgccgagc gatagcgccc tagttattga tgttctatat ataattctac caaattatgc
     7741 attattatct gcataaaagt atttttctat tctcctttta aaagcaaatc tgcttatacc
     7801 tagcatttcc gcagctttag tatggttgtt gctgcaatat ttaagagcta gtttaatata
     7861 ttcagcttcc atttctgcaa cttcattttc caaagaaaaa cctcctgaaa gcagtttact
     7921 gctcatctca tcttgactcg gctttgtaaa ttcattgcct ccgcctaagg cacttctaat
     7981 ttctaaaggc aaatcgccga tatcgatata ttcgttctct accaatatta caattctttc
     8041 aataacattt ttaagctccc tgacattccc tttccatgag tataataata gtgcttcttt
     8101 ggctctgtca gtgaatcctt ttatcttttt attaaattta cagttatatt catataaaaa
     8161 attatttgct aatactaata tatcatctcc tctttcacgt aaaagaggca gataaacagg
     8221 cactacattc aatctgtagt ataagtcctc tctgaattct ttatcttcta tggctttttc
     8281 caagttttta tttgtagcgg caataattct gatatctact tcaatatcct ctagtccacc
     8341 gaccctttta aattttcttt catctaaaaa cctcaatagt ttaggctgta cttccatggg
     8401 catttctgca atttcatcca aaaataccgt acttccgtca gctatttcaa gcaatccctt
     8461 ttttctgttg tttgcaccgg tgaacgcatt tttttcaaat ccgaaaagtt cactttctat
     8521 aagctgctga ggaatagcac cgcaattaag tttcagcatg gcagatcctc tccttatgct
     8581 gtgattatga atataagaag ctacaagatc tttgcctgta ccggtttcac cccttataag
     8641 gacagttaca ttgtcgtttt ttgataatat atcaattcta ttcataactt cattcataag
     8701 aggatggttc cctattataa ttttcttatt taattctttc tccttttctt tctccaacaa
     8761 ataaattttg ttttgcaatt ttaaattttc tgtaattctt tttattaaaa tttctacatc
     8821 aatgaggtca aagggtttgt taatatagtc aaaagcgccg agttttattg ctttaacagc
     8881 agtgttgata tcaccatagg cagtcataat aataacctcg acttttttgt ctattttttt
     8941 tattttaggc aaaatatcca atccgttctc acctgctaaa cgcatgtcta aaaaaactat
     9001 ctgaggttta tattctttta ccttttcaag agcaatatga ccggttgcag cagtacttat
     9061 gctatatccc aaatctttca gtgcttcttc caaagataat cgtatagtct cttcgtcgtc
     9121 aactatcaat attctaattt tcattactac ctccattaat gggaaacctg attattactt
     9181 tagttccttc atttactgca ctctgaatat ctatttctcc ccgattgccc attaccaatt
     9241 catatactac agataaccca agtcctgttc cttgagggtt tgtagtaaaa aaaggattaa
     9301 atacctttgc taaatcttgt gattgtatcc cacatccatt atcatgaaaa ataacttcaa
     9361 taaatttttc tttttgtgag caattattaa aagaaatatt taaagtacct cctttatcca
     9421 tagcaaaaaa agcattattg ataatattga tgaatatctg ctcagtttga gccttgtcca
     9481 catatgcaac agtatttttg cagttattat caatatttat ttttatatta ttttttttgg
     9541 cagtctcccc gattagattt aacgacttat ctaaaactcc cattaaatct gttttttcaa
     9601 tattaggaaa acttggctta gcaaaattca ataaatcagt aatcaaatta ttaagtctgt
     9661 caatttcaaa aagtactccg ttaagtaatt ttttattaga agctatcata tcgttatcct
     9721 ctttgcataa cctgtttttt aaaacttgga tacttgtttt catgcctgcc aagggatttc
     9781 gtacttcatg agcaatacct gatgcgaatt ggcctatggt agttaatcta tccaatgtgc
     9841 tcatgctgtt ttcgattctt tttctttcgg taatatcgtt aaaattgcat atagctccgc
     9901 tgatagaccc atctcctcct atcaaaagag aagttgtaac atctatatat tttttatttc
     9961 catgctcatc atcaaaagca aggattttat ttattttcat ctttgtattt aatgtttcca
    10021 ttatttgttc atgcaaacat tccttgacaa gaccgatttt ctcggctgcg ggaatcagta
    10081 aattttctgc ggtctgattt attgatatta cactgccatg ctggtcagca gttactattc
    10141 ctgctgatac gtttcttaaa atatcttcgt tgaatttggt tgcttcaatt aatctgtctg
    10201 tattgttttg cacagtatcg agcatattgt taaaagcatc agccaatgtt cctatttcat
    10261 catttctgtc tatttctatc ctatctttat aaattccgtc agcaacgatt ttattacagt
    10321 gttcagccag cattttaatc ggttttgata tattgtgcgt aatgaatatt gatgcatgca
    10381 tagaaaatac aagggcaatt aatgcaacta agattatata ttgttgactt tccattactt
    10441 caattaataa gttatctcta tttaagccgt atgctattac ccaattccac ttatcatact
    10501 tttgatatgt aaaaatataa ttgcctacag atattgactc cggtccttta tttaattctt
    10561 cattcataaa ttcttcaatg aagttattat tcaggacagt atttaacaaa atttcatcat
    10621 catcatcgct gaaaataatc aaattgtttt tgcccaggtc tttataaaaa ttaataataa
    10681 aatcttttgc tccaatttca tcctttatgt catcattaac tttttcaata gtaagaatca
    10741 tctcttctaa attacggtat gaaatattta tatgattatc caacaataaa ttatatccat
    10801 taacaacaaa tacaataaca attattaaaa ttgataccat caatataata ataaaaggaa
    10861 tgagtatctt tttttcaata ctgtttctca taatttttgc cttccctttt tacgagcgta
    10921 ttcacatgat tataactgaa atcaaaatgc tttctctaaa tcatctctac tatgattaca
    10981 caattaccat aataaaagaa ttcaacaata aataatagaa tcccgcctat atatcggaaa
    11041 tgtagcatta cactacactt ccaataagag gattctatta tattgtacca taaagtaaga
    11101 ctaaagaaaa tatttttata gaataacaat accttgttat tttgttattt tttccaaacc
    11161 tctcatgtaa tccctcaaga cattaggaat agtcactgta ccatcttcat tctgataatt
    11221 ttcaagaata gcggcaaatg tccttcccac agcaagtccg gagccgttta gggtatgaat
    11281 atactctaat ttaccttttt ctgcaggtct aaacttgata ttggctcttc tagcttgaaa
    11341 atcttcaaaa ttcgagcaag aagaaatttc aacatatctg ttgtagcttg gcatccatac
    11401 ctccaaatca taggttttgg ctgagctgaa accgatatct cccgtacata gctctacaac
    11461 cctgtaagga agacctaatc cctttaaaat ttcttcagca aaatttgtca gtatttcaag
    11521 ctggttataa gaatcttcag gtttggcata cataaccatt tcaaccttat caaattgatg
    11581 atttcggatt aggcctcttg tatctctccc ggcagaacct gcttctgccc tgaaacatgg
    11641 cgtataagca gtcatataca ttggaaggtc cttttcatcc agcacttcat ttcggtatat
    11701 atttgttacg ggaacttcag cagtaggaat taaataatat tccctttgat taagcttaaa
    11761 catgtcttct tcaaatttag gaagctgtcc tgttcctatc atactgtccc tatttaccat
    11821 aaagggagga gaaatttccg taaagccgtg gtctaaggtg tgcttgtcaa gcatatatgc
    11881 aataattgcc ctttccaatc ttgctccttc tcccttgaac attgaaaaac gagtgcccgt
    11941 aatttttgtt gccctttcaa aatcaagaat atccaagtct acgcctaaat cccagtgagc
    12001 ctttaattca aaatcaaact ttctcggctc cccccatctt cttatttcct tgttgtctgc
    12061 atctgaagca ccgacagtta tgtcgggatt tggagtgttt ggaatattca acagctcttc
    12121 ttcgattttc tcatcaattt cttttacctg gccatccaga acctttactt tttccgccaa
    12181 ttctttcatt ttttgaagaa tttccgtcac atcttttcct tcttttttga gaagaggaac
    12241 ctgtttagat actttgttct gctctgcctt catgttctcc acttcaacaa gtttttctct
    12301 cctcgctttg tctaatgcaa gaaccttatc caaactaagt tcggaatttc ttcttttcaa
    12361 cagttcttcc acctcattag ggttgtttct tattcttttg atatctaaca ttttttacct
    12421 ccataaataa aaactcccgc ctctttagag acgagagata ctcgcggtgc caccctaatt
    12481 attatactaa tggggggaca ttccttcatc cccggagact ttccctaaaa aaggtgtgcc
    12541 cccataccat aatataatca ctttaagaag ttaacgcctt caacgtctaa gttttttacc
    12601 cttagatact ccggaacgga ttcaacaact attataccgg ctcgcaccac ccgtcggctc
    12661 tcttaaataa gtcgctgttt actgctttcc ttcattgttt atatggataa tataataaca
    12721 ttataacttt gtcaagcttt atttaatgga ttgaaggcga tattatgcac aaggcttctg
    12781 acacatcatc atttttattg taatacatat gattgttgtt ttcaggaaca attatgctgt
    12841 ctccctttga tattgtgtat attttttctc ctacatgaca ctctatttcg ccctgtgtaa
    12901 caaaaatata ctctttagat tcatgtgtta tactatgatt gcttatccat gtttttggtt
    12961 tcattttaaa cagaaaggct tcaaactccg attgtttatg actatttttc aatattggcg
    13021 taataaactc aagttccaca ttaatattgt tcaatttaac tttagttctt tcttcttttg
    13081 gtattgttat aacatcattg gaagattcct ccgaaaataa tactgaaaga ggtacatcca
    13141 atgcatcgga cagtcttttt aataccgcta gagaaggaga tattaaatct ctttctattt
    13201 gtgatatata tcctgatgtt acgttaattt tctctgacaa ttcaacaatc gtcatatttt
    13261 tattctttct aatattcctg atttttttcc ctatcattat aataccccct gtactgctta
    13321 tactaatatt atacactaaa taaaacttat gtcacgtaaa ttttcacatt ttataccatt
    13381 aaatggtata ttaaaatgca ttgcagtttc tgctcttgtt ttaatgatag tcagcaaaga
    13441 caaatatttt acttttattg tagaaagttg gatttttgct tttcatatac tgctaattat
    13501 gatataatta tctattaata ctttgtaatt attcaattat atgcataagg ggatatgaaa
    13561 tggctgataa cagagtgtat ttggaagaaa taaaatattt ggatgaaatt ataaatttgt
    13621 taaaaaataa gttggaatat gaaaaaaacc aacttgaaaa taaaaaatcc gatttaattg
    13681 agtcaagaag agaaatgtgg gaaaatacaa cccattcatc tgccgatttt gataaactaa
    13741 ctgaccttaa tcagtatttg agcgcccttc aggctcaaac tttcacctat gccgagcttg
    13801 caaaacgtat tttaaagcat gaaaagatgc ttgacagacc ttattttgca agaattgatt
    13861 tcaccgaaga aggatatgat gatattgaaa aaatatatat aggtcttttt aatttaatgg
    13921 acgatgaaac ccatgaaatt aaaatctatg attggcgtgc acccatatcc ggcctatatt
    13981 accgaaatga agtaggtcct gtagaataca aagctcctgc cggattaata aagggctatg
    14041 tttcattaaa acggcaattc aaaattacaa aaggtaaaat ggaatacttc attgacagca
    14101 acattaacat ccttgatgaa atgctgatgg ttgctctatc aaaaaatatg acctccaaaa
    14161 tgaagacaat agtagaaaca atacaaaaac aacaggactt aataataaga gacctgtata
    14221 acgacttatt ggttgtacaa ggggttgcag gcagcggcaa gtcatcggtg gcgctgcaca
    14281 gaattgcata tttgatgtat caaggtttaa acttaaaact aagagccaat aatgttatag
    14341 ttatttcacc taatccgtta ttctcaaaat atatatcaaa tgttcttcct gaattaggag
    14401 aagatagtat caatgaatat acttttgaaa atatatttca taaactattt ggaaataaac
    14461 tatccatgaa atcaaaaagt gaaaactttg aaaatattat tactgcagaa actgaagaac
    14521 aaaggaattt tctgaggtca tacgatgatt ttaaaggctc atttgttttt tcaaaaatta
    14581 tagatagatt tatagaatat tatgagcata agcttataaa ttttgaagat gtttattaca
    14641 acggaaaaat aatagaaaaa cgcgagcttg caaaagcttt tctgttaagc ggaaaacttg
    14701 atatgcctac agccaaaaaa cttaaaataa ttgaaagccg aatattggaa aaggttcgtg
    14761 aaaataggat tgaaaggcgt aacaaaattg aaaaagcagt aaataaatta aataatcatg
    14821 aatttgaaat aatgtctttt acaagattgc tatcagctaa agaaacctta gctttaacgg
    14881 aaagattgaa gaaattcact gaatttgatg ttttcgagct ttataaaaag ctgtttgcag
    14941 atattatttt atttaaaaaa atttcgaaag gtcttgaact tccggagaat attgatgaaa
    15001 taataagtta tacaaataca actttaagcg attcttacaa tattccttat gctgatggaa
    15061 tagctctcac ttatatgaaa atcaaagcag agggttgtga tctgtttagc gatataaaac
    15121 aagttgttgt tgatgaggcc caggattatt accctattca ttatttaatt ttaaaaaatt
    15181 tatataaata tgcaagattt acaatagtag gcgatataaa tcaatcaata gaaaaaagca
    15241 acgatttgtc cttatatgat gatattattt ctattttcaa ttttgaaaag agcagtaagg
    15301 tttttctgaa taaaagttac agaagttcat atgaaatagg caagttcagt gcaaaattat
    15361 cgggagatga aaactatgcg gaatttttta aaagaaatga agaagagcct gttattttca
    15421 aatttaaaga cattaacaac ctccgggatg aaattataaa aactattgag aaatataaaa
    15481 ctgaaggatt tagctcaatt gcaataatat gtaaagatag aaaggaagcc tcagacttgt
    15541 attttaaact ggctaataaa ataaaaataa aattgataga ctatttagat tatgacagtg
    15601 ttttggaagg taccttaata cttcctgttt atttggcaaa aggtctggaa tttgatgctg
    15661 ttattgtata tgaagcaaat gataaacatt ataataacga attcgataaa aaacttttat
    15721 atgtcgcatg tacaagagca cttcacagat tatcactgtt ttatacggga aatttaacaa
    15781 aatttttgga ctaaaaaatc cgcttaaccc ttaagcggat ttgcttatat atggtcatgt
    15841 cataaagcgg gtgatgggaa tcgaacccac acagccggct tgggaagccg gaactctgcc
    15901 actaagttac acccgcaata aatggagata aggggtctcg aacccctggc ctcttgaatg
    15961 ccattcaagc actctcccaa ctgagctata tccccaaaag tgtatgtatc ttaattttaa
    16021 gaaaaaatac tatatataat ttaatatatt ttttttcatt tgtaaagtta atcttttaat
    16081 aaaaaagcta ctgaggggat tcgaacccct gacctactga ttacgaatca gttgcgctgc
    16141 ctgctgcgcc acagtagcat gtatataatt taatactttt ttggcaattt gtaaacagta
    16201 aattttgtaa gactaaattt ccgggaaata atcgtaacat gagaggttgt aacacatata
    16261 ttataatata taaattttat atagaggaga gtatatatgg acgattacaa tcagtattat
    16321 gataccatga acgattatat gtattatgat gatattaata aagatgtata cagcagtcaa
    16381 tacgatatgt atgacaggag attcgacaac aggttcgaca acaagttcga cagaagattc
    16441 gatagaagat atgacagatt tttttatgac ccgatgaggt attatcgatt cccattctgt
    16501 gacaggtacg gaagatgtga aaatcctatt tggtggctgt tctggccctt cttttttcaa
    16561 taatcagtat gacaaaggga cgctctgcgt ccctttaaaa tttgcgactt cccggtttta
    16621 ttggctttga gcccatttca catagggggc aatttttatc atcataggtt tcaaagaata
    16681 attctatcga gctgtaaaca ggaaattgaa tttcacatac ttttctatct acaatacatc
    16741 cgattcctac tactataccg cctgcctctt caagaacctt ggcagtttcc atggatgatt
    16801 tcccggttgt tacaacatcc tccattatta aaactctgtc accgggttgt atttcaaaac
    16861 cccttctaag tgtcattaca ttattttctc tctcagtaaa tattgttttt atacctaatt
    16921 gtctgcctag ctcataagcg gctgttattc ctcccatagc aggtccgaca ataacatcca
    16981 aatttaatga ttttactttt tcatatacaa gagaaataac tttttctgcc ttgtccggat
    17041 attgcagaag tttagcacat tgacaatatt tgttgctgtg cttgccggat gaaagcaaga
    17101 aatgaccttc aagcaatgca ccgcattctt tcaatatttc aattacatct atcatattac
    17161 cgcctcctgt ggctctaaat aatccctctt atttcttcta gggatttaat attttcattg
    17221 tccataaatt cttccaaatc attgagaatg tctactgcaa tatcaggttt aataaagttc
    17281 gctgttccta tttgcaccaa tgttgctcct gccataatga attcaattac atcgattgca
    17341 ttcattatac ctccaagacc gcaaatggga atattaacca tttttgcagc ttcatgtacc
    17401 attcgaagag caataggttt tacacatggt cctgaaagtc ctgcataaac attctcaaat
    17461 acggcttttc tgttttttat atcaattgcc atagcttgaa ttgtattaat taatgaaatt
    17521 gcatctgctc ctgccgactc acatgctaca gccatatccg gaatattttc cgcatttggt
    17581 gatagtttaa ccataagagg ctttttacat atcttacgaa ctcttgatac tatatcataa
    17641 gcaacggaag atttcactcc aaaagctatg cccccgcttt taacattcgg acaggaaatg
    17701 ttaagctcta cagcatccac atttgtatca ttcagcttat atatgccttc taaataatct
    17761 tcctcgcaat gtcctccaag attggcaaaa ataatagtat tatatgtttt catcctggga
    17821 agttcttctg ttataaattt gtcaacaccc gggttttcaa gtcctatgct gttcataagc
    17881 cccattggag tttcccagag tctgattcct ttattcccgc tccttggagt taatgtaagg
    17941 cctttgctgc aaattccgcc aagaattgat aaatcgtaaa tttgagaaaa ttcttctccg
    18001 aaaccaaaag ttcctgatgc cgctattatg gggtttttaa atttaagatc cattgcttcg
    18061 gtttttaaat ttgccatatt tctcctctat attaattctt cactgtcaaa tacaggacct
    18121 tccttgcaag ctcttttatt gcccgtcttc gtttcgaccg tacaccccaa acaagctccc
    18181 ataccgcatg ccatccgcct ttccaacgat acatagcatt taattttttt gtccctgcat
    18241 atagttgcaa tcttactcat cattatttcc ggcccgcagg ttacaacaac attataatca
    18301 tcataagaaa tataatccgt tacgtaacct ttttctccaa agcttccatc ctccgtaaca
    18361 acgatgcatc tgcgggctga agatttaaat tcatcaatta tatacatatt ttctttatcc
    18421 ctgtatccta aataaacatc tgctttttta cctaagactt tacataaata caatagtgga
    18481 gcaacaccta tgcctcctcc tattactgca actctgccta acagtttatc cggctcaaag
    18541 ccctttccca agggaccgaa tatctgaatt tcgtcatttt ttcgcaggct gcttattttt
    18601 tttgttcctt gcccttccag tctatataaa aatgaaacaa gattctcatt tacatcatta
    18661 acgcttatgg gtctcggaag caaaaatgca ttatcagtgg ttttcagcat gaaaaattgt
    18721 ccgggtttta tttccgaatc aaattttacc gaaagcctat aaatcttttt ggaaatttgc
    18781 tcgttttccg caacagcggt tactaatacc ttcattattt tctccaattg cttaccttta
    18841 cttcttatca taagccgtat cgcagtatac gcacctgtat attccttttt ccctgtcagc
    18901 aagtctgaat ttatgaacta tttcctgctc tattgatgtt atgcatctcg ggtttttaca
    18961 tttaactaca ttttcaactt cctgaggcaa ttctaaatta attttgtcta ttataacaga
    19021 attgtctatt acattgattg ttatgtttgg gtcaataaaa ccaagcacct taaggtccat
    19081 atccagattg ttttctactt ttattatatc tttttttccg tatttcttgc tttttgcatt
    19141 tttaattatg gcaacactgc aatccatctt atcaagattc aaataatgat atatttccat
    19201 tgccttgcct gccttaatat ggtcaattac caatcctttt tctacactgt ctatattaag
    19261 cattattcca cccccaatag tttcaatatt aaagccattc ttacatacat accgcattta
    19321 gcctgtataa aatatgctgc tctcggatca ttgtcaactt ctaccgatat ttcattgact
    19381 ctaggaaggg gatgcataag aatcatatct ttttttgcat ttgacatttt tttattgtct
    19441 acaatatagc tgtcctttaa tcttatatag tccgcttcat tgaaaaatct ttctttttga
    19501 actcttgtca tataaagaac atccaact
//
LOCUS       DAOPUE010000030        19090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2924_length_19090_cov_9.940320, whole genome shotgun sequence.
ACCESSION   DAOPUE010000030 DAOPUE010000000
VERSION     DAOPUE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19090
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2924_length_19090_cov_9.940320"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..908)
                     /locus_tag="PKH42_04355"
     CDS             complement(24..908)
                     /locus_tag="PKH42_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOA19694.1"
                     /translation="MMKALYSGVSGMRSHQTKMDVIGNNIANVNTYGYKTQRTTFRDV
                     YYQTLRSPGAPNEDRGGTNASQVGYGAQVGSVDTIHTISGYSPTYKSTDLYINGDGYL
                     AVLNTSGDTLYTRLGALNFDEDGNLVDVNGSRLLGYDNDSTGTDNEIELDGDGNINIT
                     ATLAKTIGAIKIADFENFKNITINSDGSITAVDDTDDTIKTIGFIPIFKIPNQDALIL
                     EGNSYYSVGNNAGNPIANAPGAGGTGALVTGGLEMSNVDLANEFSDMIITQRGFQANT
                     KIISVVDQMLEELVNLKR"
     gene            complement(987..1361)
                     /locus_tag="PKH42_04360"
     CDS             complement(987..1361)
                     /locus_tag="PKH42_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HOA19695.1"
                     /translation="MTDINFIKKISSLNPNIQPNYNAHRNDSNNFKELLSEKLKNEVS
                     FSKHANERIQQRNIDVSQEVTEKLNEAAVQAKDKGLKNVLVMIDNQAFILSTMNNKVI
                     TAVNSDDLKENIFTNIDGAVIK"
     gene            complement(1374..1943)
                     /locus_tag="PKH42_04365"
     CDS             complement(1374..1943)
                     /locus_tag="PKH42_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOA19696.1"
                     /translation="MEINAYNSSYKVNNINTANDLKIQKNNEGLNMQDFLNLLVAQIT
                     NQDAMNPMDNTEFIAQMAQFSTLQAMTDLTEMALQGQATSLIGKNVVVADYNGNGGLT
                     IDEGIVQRVTLHGGKTKLYVNDMEYEYSNVMEIKEAEKAEENFLTEMLGNISEKIDNL
                     NNTNNSNISPTDEIPPIDEEEALPYDETQ"
     gene            complement(1933..3012)
                     /locus_tag="PKH42_04370"
     CDS             complement(1933..3012)
                     /locus_tag="PKH42_04370"
                     /inference="COORDINATES: protein motif:HMM:NF021032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOA19697.1"
                     /translation="MNVNSLTISQLPGKVIRSDYSNSVNKNMFENMLNNAMAENQNMM
                     ELLGKNFGSILAANSIEIEDYSIEHENKDETQKQNFYENIYGIADIFFFRPNAAIEEV
                     TAENKITNVDYNFIDAKIQNGTDITAGSALKNLVSEIEIQRDLGISNDIKSSNEVQSM
                     KTILELGKKQKNEPAYLEMPDCIDLKNKIITISDGSTEIKSQVLSQVKDKIIIMTENS
                     QDSNGIKTITMELRPQALGKVDIKMSYENNKLTVEIKASNEETQKILSSNTDELKELL
                     NKTSETDVKIIVKPYEPKNQNILNYQDNRQGSENFYQDNEQNNQGRQRNKYYYDNDIK
                     TKEEDVFSELIDLNYSRIKEGMHGN"
     gene            complement(3203..3655)
                     /locus_tag="PKH42_04375"
     CDS             complement(3203..3655)
                     /locus_tag="PKH42_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19698.1"
                     /translation="MKKFVFSLQKVLELKNQLLENMKIELTNLNRECEKIEIFIKCLK
                     IKFAEIENEYVEKSYKSISAGEMSYYKMLSESILVQIENKEIEKAIVLKKIADKRQEI
                     ISMNTEISSLEKLKEKELEKHSKECLKKEELFIEEFVSNKTMTNEYAI"
     gene            complement(3698..5005)
                     /gene="fliI"
                     /locus_tag="PKH42_04380"
     CDS             complement(3698..5005)
                     /gene="fliI"
                     /locus_tag="PKH42_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOA19699.1"
                     /translation="MQVKEKFNDIRETLIKEDFYTHIGKVKRISGLMIEAHGGKYKIG
                     EICEIKTASNKTIRAEVVGFNDDRVLLMPYEDIKGIGLGNLVVSTNNKLKIPVGDFLI
                     GRIIDATGQAIDGGEKFTSQEYCYVDNNYINPLSRPRISEPLSFGVKAIDGLLTIGKG
                     QRMGIFSGSGVGKSTLLGMVAKNVKADINVIALVGERGREVREFIEKDLGEEGLRRSV
                     LVIATSDQPAMLRVKCALTATTIAEYFKDKGKDVLLMMDSLTRFAMAQREIGLAAGEP
                     PVSRGYTPSIYAELPKLLERSGNFQEGSITGVYTVLVEGDDTNEPISDTVRGILDGHI
                     VLNRKLANSNHYPAIDINASISRLMDDIATEEHNNKAKKLRDILAEYYSNYDLISIGA
                     YKKGSNVKLDEAISKIEEVNNFLKQGVNEKYNFDEIINLMDKI"
     gene            complement(4995..5750)
                     /locus_tag="PKH42_04385"
     CDS             complement(4995..5750)
                     /locus_tag="PKH42_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOA19700.1"
                     /translation="MSNIIKAEYVICDIDKQKNNHIHRQVSTYREDLFRIYNQREILL
                     KEAEEKAAEIVSTAIENAQLEITECKKNAYEDGYNAGMEAGRNKGYKEGYDTGKSKIS
                     EILLEQNKEKLNEIAEMIKTIESEKLKIISRYEEELIKLSIEIAEKIIRHEIDTKDDV
                     VSGIIKNAIKDYRNVDWIKIYLSDKDDAEIIQADKELISELEKISKDIKIEVMDELQK
                     GGAIIETADGIVEASIDTQLKNLKEMVLNKNAG"
     gene            complement(5743..6747)
                     /gene="fliG"
                     /locus_tag="PKH42_04390"
     CDS             complement(5743..6747)
                     /gene="fliG"
                     /locus_tag="PKH42_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOA19701.1"
                     /translation="MAERLTGKQKAAAIIISLGAEDASRIYKFLKEDEIEQLTYEISR
                     LQHLSPQTMESTLKDFYELCLTQKVITEGGLEYAKNVLEKAFGTQVATSLLERVTKTL
                     RNKSFDFIRQADYKNLLTIIQNEHPQTIALILSYARADQAAAVIAELPKEKRIDVVER
                     IAKMDRTSPEIVKYVEKELLKKFNSMVSIDYTEIGGINYVADIMNNMDRGNEKYIFDE
                     LNKKDAKLSDEIRKKMFIFEDIVTLDSLSIQRFLREVDSKDLVLSIKGSNKDVSDVLF
                     NNMSTKMADTIRSELEYTHNVRLRDVEEAQQRIVSVIRRLEEEGEVIIAKSGKDDIIV
                     "
     gene            complement(6749..8311)
                     /gene="fliF"
                     /locus_tag="PKH42_04395"
     CDS             complement(6749..8311)
                     /gene="fliF"
                     /locus_tag="PKH42_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOA19702.1"
                     /translation="MSEQLKNIWKNVTEFWGKLNKRKKSLIIGGLLVLVIGALIITFI
                     LNNKDYVVLFRNMDEAESVEVMKQLQESKIDYKYQTDGTILIPKEQESLLRMQLAQAG
                     HPRTGINYDIFTENIDFMTTDYEKRQYEIFQLQERLQSSIETIDGVKEAIVTINLPQD
                     KSFAWETDKKESSASVKINLQNGYSLSPSQTNGIMQLLVKSVKGLKEENVAIIDTNGN
                     SLNISNEMLQTNTIKLKLSIEREFERETEEDVTEFLSRIYGPENVKVSAKCTINFDKK
                     ISEILQYLPDEETRLGVPGDTHSEKEITGPGETTGGIAGTETNAEVPTYPGVVVSGDD
                     IYFRDSNTINYLVSQLKEQIEHNPGTIEKLTVAVVINKANMSDKEAGEINQLIAFAAG
                     IGTEDIALSNMVFYDLEEPAVPVAATGIFERIQLKELLIYGGIALIAISILLIILILV
                     LRKRKKKKALDNAAITSDTDSSEYSWADIQDGIKVQETEEQAIKKQLKDFTKTSPEIV
                     SQLIRTWLKGDE"
     gene            complement(8338..8634)
                     /gene="fliE"
                     /locus_tag="PKH42_04400"
     CDS             complement(8338..8634)
                     /gene="fliE"
                     /locus_tag="PKH42_04400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOA19703.1"
                     /translation="MFIENIKPINASQINELNKPKEKISELAFKSIFDTAIENYKNAE
                     AEVDKDIYKLSIGETDDLHNLMINVEKAQISLDLVIQLRNKALEAYNEIMRMGV"
     gene            complement(8637..9068)
                     /gene="flgC"
                     /locus_tag="PKH42_04405"
     CDS             complement(8637..9068)
                     /gene="flgC"
                     /locus_tag="PKH42_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOA19704.1"
                     /translation="MSFLKTLNISGSGLTAQRARMDVISENIANIDTTRTEEGGPYRR
                     KMVVLKTSGDFKNMMVKNLNQYEPGGVEVAEIMEDNSEFKAVYNPEHPDADENGYVNM
                     PNVDSLKETIDIMEAFRAYQANITALNTVKQMAIKALEIGR"
     gene            complement(9137..9520)
                     /gene="flgB"
                     /locus_tag="PKH42_04410"
     CDS             complement(9137..9520)
                     /gene="flgB"
                     /locus_tag="PKH42_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOA19705.1"
                     /translation="MRVNVNLNLLKKSLDGLWLRQQTTMENIANYSTPGYKSKTVDFE
                     TILSANLKNTYGKTTSEINEQILSGNIIIKENDNLSMRLDGNNVDLEKENLQLAKTQI
                     QYMYTVAEINSYFSKLKSVISEGKK"
     regulatory      complement(9646..9728)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(10026..10262)
                     /locus_tag="PKH42_04415"
     CDS             complement(10026..10262)
                     /locus_tag="PKH42_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOA19706.1"
                     /translation="MLVIKRKVSESILIGDDIEIIISEISQDKVKIAISAPQDIKITR
                     KELAETCEFNKIASEKINEISLNDIKNKLKAIKN"
     gene            complement(10256..10708)
                     /gene="fliW"
                     /locus_tag="PKH42_04420"
     CDS             complement(10256..10708)
                     /gene="fliW"
                     /locus_tag="PKH42_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOA19707.1"
                     /translation="MSEKMKIKTRDFGEILVGEEDIINFTTGIYGFDDYKKYIILKDS
                     PEDDIMFLQSLDNMELSFVLIDPYAIFIDYNPILNEDDLCELKSTGTAELKYLVIAII
                     KDDIKESVVNLKSPIAINPNNRTAKQVILQNEYPLRYNIVSTEEGNKC"
     gene            complement(10701..11306)
                     /locus_tag="PKH42_04425"
     CDS             complement(10701..11306)
                     /locus_tag="PKH42_04425"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HOA19708.1"
                     /translation="MEPLLEIKTIPMAFEMKINRARLELATTDATFEVSRDKGGLQMQ
                     MKPAKIKIDTVEARYSAGLKSAMHLISDVAKKGVQAAYEATATYAREGNLMLDINIMD
                     NPIPEIAMKKFISDVSFNIGFIPSTGPDISWDTGGISMSFAMDKLDFDWNVEKPQIKF
                     IPGSIEFIISEYPSVEIKYVGTPIFVPPSANPNYKEIDTLV"
     gene            complement(11322..12314)
                     /locus_tag="PKH42_04430"
     CDS             complement(11322..12314)
                     /locus_tag="PKH42_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19709.1"
                     /translation="MRITTSMMSNKYIKNLNKSAYEMNYLSEKVETGRKFVKGSEDPV
                     SAIKAYKLRREYRTTEIYDTNIRDVESFLTAAETNLTEISNNMESVYTSYLKGINGTM
                     NDEDREIIAKQLENLQRSILTSLNAKFEDRYVFGGTNKEEIPFTLDMNGNLLFKGLDV
                     NDPANKEALDKLANETINIDLGLGMTFNGDGELNTDTVFDMSMSGIKFMGYGGTDDNP
                     ENLYNLIGKIKDELRNPDFSIDKISPYIDRFEEQKKQVLVHITDIGAKTNYLDFLKTR
                     NEDNQFNLNKKLLEVEFEDPAEAIVNFKMQEYSYNAALSMGNRILQNSFIDFMK"
     gene            complement(12325..13941)
                     /gene="flgK"
                     /locus_tag="PKH42_04435"
     CDS             complement(12325..13941)
                     /gene="flgK"
                     /locus_tag="PKH42_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOA19710.1"
                     /translation="MLRSTFLGYKTATSALKVNQNHMDVVGQNISNINTKGYTRQRLD
                     ISSVAFNTSNLKYGTPGPVIGQGVNANGVSQFRDAFLDLRYRMETSKAGSEQVQLEAL
                     RDLESVFDEISMDKLDAQFSDLLTQLHSLTSSPSDPVMEGVVRTSAEILTQMFNDYSR
                     DIETIYNQQLNYLENGAVEKVNELTKNIAKLNQQIKESNISGNPALELNDERNMLVDE
                     LSNYIDIEVDIEPLNIGGGKTIDELVIRLSGTTYELVNRNNSNTISVNEQNGLIKIEL
                     TGATDTDDITNFIKGGQIDGYLKFLNGKGEFATVDDIDPVNGYEKAAEVKGIQYYNNM
                     LDTLANKFATVMNELNRKPDSFDIDGNVQTWITKPLFGARGADPSDPDTEIIRAGNIK
                     ISDDWASATDSYITNTIKEKAGVDEDNTGAVDNILRMINAFNAKNEFDVDRDPSTTND
                     ILFKGTFQEFLSYTTTKLNLQTADVQISFDTYSETQFHIDYARSSMSSVDLNEEGVNL
                     LQYSKSYNAAARLMTTLDEMLETLINRMGV"
     gene            complement(13961..14416)
                     /locus_tag="PKH42_04440"
     CDS             complement(13961..14416)
                     /locus_tag="PKH42_04440"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HOA19711.1"
                     /translation="MDSLYQLLKEYTEIYKMFLELEYDKYEAVIEDDVIALDNIVSQE
                     EVYYLQMKGIEHKREKLLNSLELSGKTLKEIIDAAENESKIKLQEQFEELNNKIKELN
                     KINDLLKIVIEVRLRRINNKMNNLGEKENIYTNDKSKNVTEGLLISHKI"
     gene            complement(14426..14704)
                     /locus_tag="PKH42_04445"
     CDS             complement(14426..14704)
                     /locus_tag="PKH42_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOA19712.1"
                     /translation="MKINNINNNNYINYKGNSNLIKKEEINKENKYDVIDFNNKNKEN
                     ISLQSIKKKVVSQINEETNADKINRIKDSINNKTYSIDVEEIVRRLLR"
     gene            complement(14775..15254)
                     /locus_tag="PKH42_04450"
     CDS             complement(14775..15254)
                     /locus_tag="PKH42_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOA19713.1"
                     /translation="MNNENIMQMREVIPWLIFKLNETLYTVNSKYITSIVIKPDDVTF
                     VPNVAEHIIGLIHLRGNVIPLVDLKLLLNTEDESGNNSANNSKENKAMVVVLEKGNSF
                     IGLVVDEVLSVENIFAYEETEEIKQMCNNGFITGVAKGHKSEEVLLVIDEEKIMSNA"
     gene            complement(15247..15771)
                     /locus_tag="PKH42_04455"
     CDS             complement(15247..15771)
                     /locus_tag="PKH42_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOA19714.1"
                     /translation="MDKFNSLHTYIEEEDAQVNKYLTFYIENQVFGIPIAHVVQITGM
                     QEIEEVPEFPSYAKGIINLRGIIVPIIDVRIRLKKQEIEYNERTCIIVTNIEGLYIGF
                     IVDSVNEVINIYYENISEPPQVGNNYVITYITGIAKLDKKIVILIDLNKLLNEKEIGS
                     LELIKDEIMGDYNE"
     gene            complement(15924..17549)
                     /gene="groL"
                     /locus_tag="PKH42_04460"
     CDS             complement(15924..17549)
                     /gene="groL"
                     /locus_tag="PKH42_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOA19715.1"
                     /translation="MAKIIKYDEDARRAMERGVNALADTVKVTLGPKGRNVVLNKSFG
                     SPQITNDGVTIAREIELSDPYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPMILKKGIEKAVAAAVEELKNITKKVETSEEIAQIASISAGDEEIGKLI
                     SQAMDQVGKDGVITVEESKTMDTELVVVEGMQFDRGYLSAYMVTNTEKMEAVLEDPYI
                     LITDKKITNIQDVLPVLEQIVQTGKSLLIIAEDVEGEALATLVVNKLRGTFNCVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISEELGLELKETTLDMLGNAGTVKIDKENTVIVNGA
                     GSKEEIEERARQIRAQHDESTSEFDREKLMERLAKLTGGVAVINVGAATETEMKEKKL
                     RIEDALNATRAAVEEGLVSGGGVALLTTIDKVNKVAEGLDGDAKTGAKIIEKALEEPL
                     RQIAINAGLEGSVIVEKIKNSEKGVGFDALNEKYVNMIEAGIVDPTKVVRSALQNAAS
                     VAAMLLTTEAAVTDEPKKEEPAMPPMGGGMGMM"
     gene            complement(17565..17846)
                     /locus_tag="PKH42_04465"
     CDS             complement(17565..17846)
                     /locus_tag="PKH42_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOA19716.1"
                     /translation="MTVKPLGERVVIKMIEAEEKTKSGIVLPGSAKEKPQVAEVLAVG
                     AGITNDEKKKDEIKVGDKVIYSKFAGTEVKIDGEELIIIKLADVLAVIE"
     gene            18201..>19090
                     /gene="fba"
                     /locus_tag="PKH42_04470"
     CDS             18201..>19090
                     /gene="fba"
                     /locus_tag="PKH42_04470"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOA19717.1"
                     /translation="MALVTSQEMFKKAYEGKYAVGAFNVNNMEIIQGIVEAAKQENAP
                     LILQVSAGARKYANPIYLRKLVEAAIEDTGLDICLHLDHGEDFEICKQCVDDGFTSVM
                     IDGSKYPFEENIALTKRVVEYAHSKGVVVEAELGKLAGVEDAVKVRTKDATYTDPDEA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGDPTLDFERLETITNLLPGFPLVLHGSSSVPK
                     EFVDLCNKYGGQIPGAAGVPEEMLRRAAQLGVCKINIDTDLRLALTACIRQFFVEHPS
                     EFDPRKYLGPGRTAIKDMVA"
BASE COUNT         5036 a         3753 c         2730 g         7571 t
ORIGIN      
        1 ttactgtttc gttactaatc tatttacctt ttcaaattaa ccagttcttc aagcatttgg
       61 tcaacaactg aaataatctt ggtattagcc tggaatcctc tttgagtaat aatcatgtcg
      121 gaaaattcat ttgccaaatc tacgttggac atttcaagtc ctccggttac caaggcacct
      181 gttccgcctg ctcccggtgc atttgcaatt ggatttcctg cattattccc tactgaataa
      241 taagagtttc cttctagaat cagagcatct tgatttggta ttttaaaaat cggaataaat
      301 cctatcgttt taatcgtatc atcggtgtca tcaacggctg ttattgaacc atcactgtta
      361 atagtaatat tcttaaagtt ttcgaaatca gctatcttaa ttgcacctat agtcttagcc
      421 aaggtagccg ttatattaat gttgccatcc ccatcaagtt ctatttcatt atcagtaccg
      481 gttgaatcat tgtcatatcc taagaggcgt gagccgttta catctaccaa attcccgtct
      541 tcatcaaagt ttagagctcc taagcgtgta taaagcgtat ctccgcttgt attcaaaact
      601 gccaaatatc catcgccatt aatataaagg tccgttgatt tatatgtcgg cgaatatccg
      661 gatattgtat gaattgtatc aactgaacct acctgtgctc cataaccaac ctgtgatgca
      721 tttgttcctc ctctatcttc atttggtgct cccgggcttc gaagtgtttg ataatataca
      781 tctctaaaag ttgttctttg tgttttatat ccatatgtat taacgtttgc tatattgttt
      841 cctattacgt ccatttttgt ttggtggctt ctcattccgg atacaccgga gtaaagtgct
      901 ttcatcataa tttttattct ccttttaatt ttctttgctg ttagtccgtc aagatatcag
      961 gaccttagcc tccttaaaag gaccggctat tttattacag ctccatcgat atttgtaaaa
     1021 atattttctt ttaagtcatc actgttcact gccgttatta ctttgttgtt cattgtgctt
     1081 agtataaatg cctgattgtc tatcattaca agaacatttt ttaaaccctt gtccttggcc
     1141 tgaacagctg cttcattaag tttttccgtt acttcttggc ttacgtcgat atttctttgt
     1201 tgaattcttt catttgcatg ctttgagaaa ctaacttcat tttttaattt ctcactcagc
     1261 agctctttaa agttgttgct gtcatttctg tgtgcattgt aatttggctg aatattggga
     1321 tttaagctgc ttattttttt tataaaattt atgtctgtca tatcatcacc tgcttattgt
     1381 gtttcatcat acggcagtgc ctcttcttca tcaatcggcg gtatttcatc agtaggcgaa
     1441 atatttgaat tattggtatt gttcaaatta tctattttct ccgaaatgtt tcctaacatt
     1501 tctgtcaaga aattttcctc cgctttttca gcttccttaa tttccattac attggaatac
     1561 tcatattcca tatcatttac atacagcttt gtttttccgc cgtgcagcgt aactctttga
     1621 actattcctt catcaattgt taatccgccg ttaccgttat agtcggcaac cacaacattt
     1681 ttccctatga gtgatgtagc ttgtccctgc aatgccattt ccgtcaggtc cgtcatagcc
     1741 tgaagcgttg aaaactgcgc catctgtgct ataaattccg tgttgtccat tggattcata
     1801 gcatcctgat tagtaatctg agctacaagc aaattcaaaa aatcctgcat atttaaacct
     1861 tcattatttt tttgaatttt taaatcattt gctgtattaa tgttgtttac tttgtagctt
     1921 gagttgtaag cgttaatttc catgcatacc ctccttaatc ctggaataat ttaagtcaat
     1981 aagctctgaa aatacatctt cttcttttgt cttaatatcg ttatcatagt agtatttgtt
     2041 gcgctgcctg ccttgattat tttgttcatt gtcttgatag aaattttcgc tgccttgccg
     2101 gttgtcttga taatttaaaa tattctgatt ttttggctca taaggcttca cgatgatttt
     2161 tacatctgtt tctgatgttt tatttaacaa ctcctttagc tcatctgtat ttgaagatag
     2221 tattttttga gtttcttcat ttgatgcttt gatttctacc gttaacttat tattttcata
     2281 gctcatttta atatctacct tacctagagc ttgaggtctg agctccatgg ttattgtttt
     2341 tatgccattt gaatcctgac tgttttctgt cataataatt attttgtctt ttacttgaga
     2401 tagtacttgt gacttaattt ctgttgagcc gtcactaata gttattattt tgttttttaa
     2461 gtcaatacaa tccggcatct ctaaatatgc tggttcattt ttttgttttt tgccaagctc
     2521 aagtatagtc ttcatacttt gtacttcatt tgagcttttg atgtcattgc tgattcctag
     2581 atctctttgg atttcaatct ctgacaccaa atttttaagt gctgagccgg cagtgatatc
     2641 agtgccgttt tgaatcttag catcaataaa attataatcc acatttgtta ttttattttc
     2701 ggctgtaact tcttcaattg ctgcattggg cctgaaaaag aaaatatctg caataccata
     2761 tatattttca taaaaatttt gtttttgtgt ttcatctttg ttttcatgct caatactata
     2821 atcttcaatt tcaatactgt ttgcagccag aatgcttccg aagttttttc ctagcaactc
     2881 catcatattt tgattttctg ccattgcatt attgagcata ttttcaaaca tatttttatt
     2941 cacgctgttt gaataatcac ttcttataac cttaccgggc aattggctta tcgttaaact
     3001 gtttacattc aaattctcac ctcctttcaa tataattcag cagttgggga cacacacgag
     3061 gagtgtcccc gtacttacat tcatatttcc ctcctttcta ttgatattca atggtttcac
     3121 ctgtagttgt tatcccgggc tcagactttg agatccttcg ctgcgctcag gatgacgcgg
     3181 gctgcgccat caggtctcca aattatatag catattcatt tgtcatggtt ttatttgaaa
     3241 caaattcctc tatgaaaagt tcttcctttt ttaaacattc cttgctgtgt ttttctaatt
     3301 ccttttcttt tagtttttct aaacttgaaa tttctgtatt catactgatt atttcttgtc
     3361 ttttgtctgc tattttcttt aatactattg ctttttcaat ttccttattt tcaatttgta
     3421 ctaatatgct ttcactgagc attttataat aagacatttc acctgctgat attgatttat
     3481 aggatttttc tacatattca ttttctatct ctgcaaactt tatttttaag catttaataa
     3541 atatttcaat tttctcacat tctcgattta agtttgtgag ttcgattttc atattttcca
     3601 acagctgatt ttttaattct aaaacttttt gaagtgaaaa aacaaatttc ttcatttcct
     3661 gcctcgttta aaatggtatt tttgtaacta gaaagtatca aattttatcc atcaaattaa
     3721 ttatttcatc gaaattatat ttttcattta caccttgttt taaaaagtta tttacttcct
     3781 caattttact aattgcttca tccaatttaa cattgcttcc ttttttgtag gcacctatgg
     3841 aaattaaatc ataattagag tagtattcgg ctaatatatc tctgagtttt ttggccttat
     3901 tgttgtgctc ttcggttgca atatcatcca taagtcttga tatacttgca tttatgtcga
     3961 ttgccggata atgatttgaa ttagccaatt ttctgtttag tactatatgt ccgtcaagta
     4021 ttcctctgac ggtatccgaa ataggctcat tggtgtcatc tccttcaaca aggactgtat
     4081 aaacacccgt aattgaacct tcttggaagt ttccgctcct ttctaacagc tttggcagct
     4141 cggcataaat tgacggggta taccctcttg atacaggcgg ttcacctgca gccaggccta
     4201 tttctctttg agccattgca aatcttgtca aggaatccat cattaataaa acatctttac
     4261 ctttatcttt gaaatattct gctatggttg ttgctgttaa tgcacatttt actctaagca
     4321 ttgcaggttg gtcggatgtt gctattacca atacggacct tcttaatcct tcttctccta
     4381 agtctttttc aatgaattct cttacttctc ttcctctttc tcctaccaat gcgatgacat
     4441 ttatatctgc ttttacattt tttgcaacca tcccgagaag ggtgcttttc ccaacaccgc
     4501 ttcccgaaaa tatgcccata cgctgtcctt ttccgatggt taaaagtccg tcaattgctt
     4561 ttactccaaa gcttaaaggt tcgcttatcc taggtcttga caaaggattt atataattat
     4621 tatcaacgta gcaatattcc tgactagtaa atttttcgcc gccatcaata gcttgtcctg
     4681 ttgcatctat aattctgcct atcaagaaat ctcccacggg tatttttagc ttgttgtttg
     4741 ttgatacaac gagattcccg agacctatgc ccttaatatc ctcatagggc atcagcagca
     4801 ctctgtcatc attgaagccc acaacttctg ctctgattgt tttgtttgat gcagttttta
     4861 tttcgcatat ttctccgatt ttgtacttgc cgccgtgtgc ttctatcatc aatcccgata
     4921 ttcttttaac ttttcctata tgtgtataga aatcttcttt tattaatgtt tctcgaatat
     4981 cattaaattt ttctttaacc tgcattttta ttaagtacca tttctttaag attttttaac
     5041 tgggtatcaa tacttgcttc gactattcca tctgctgtct cgattattgc accgcctttt
     5101 tgcaattcat ccataacttc tatttttatg tctttagata ttttctcaag ttcacttatt
     5161 aattctttat ctgcttgaat gatttctgca tcgtctttat ccgaaagata aatctttatc
     5221 caatctacat tgcggtaatc ttttattgca tttttaatta ttcccgatac aacatcatct
     5281 tttgtatcaa tttcatgcct gataatcttt tctgctattt ctatagagag tttgattaat
     5341 tcttcttcat atcttgaaat tatttttagt ttttcggatt ctatggtttt aatcatttca
     5401 gctatttcat ttaatttttc tttgttttgc tctaagagta tttctgatat cttagatttt
     5461 cccgtatcat atccttcttt gtagcctttg tttctgcctg cttccattcc tgcattatag
     5521 ccgtcttcgt aagcattttt tttacattct gttatttcca attgggcatt ttctattgca
     5581 gtactgacaa tttcggcagc cttttcttcg gcttctttta gaagaatttc tctttgatta
     5641 taaattctaa acaaatcttc cctgtaagta ctcacttgcc tgtgtatatg attgtttttc
     5701 tgtttgtcta tgtcacaaat cacgtattcg gctttaatta tattagacaa taatatcatc
     5761 cttcccgctc ttagcaatta taacttctcc ttcttcctca aggcgtctga ttactgatac
     5821 aattctttgc tgagcttctt ctacatctct taagcgtaca ttgtgagtgt attcaagttc
     5881 tgaccttatt gtatctgcca tcttagtgga catattattg aataatacgt ccgaaacatc
     5941 tttatttgaa cctttaattg acaataccaa gtctttgctg tcaacttcac gaaggaatct
     6001 ttgaatactc aatgaatcta aggttacaat atcctcgaat atgaacatct tctttctgat
     6061 ttcatcactt agcttagcat cctttttatt gagttcatca aatatatact tttcgtttcc
     6121 tctgtccata ttattcataa tatcagcaac atagtttatg ccgccgattt ccgtataatc
     6181 aatagatacc attgaattga atttcttcaa taattctttt tccacatact ttacgatttc
     6241 cggtgatgta cggtccattt ttgctattct ttctaccaca tctattcttt tttcctttgg
     6301 caattctgca ataactgccg ctgcttggtc tgcccttgca taagatagta ttaaagctat
     6361 tgtttgagga tgctcgtttt gtataatggt taataaattt ttgtaatcag cttgtctaat
     6421 aaaatcaaac gacttattgc gcagtgtctt tgttactctt tctaaaagac ttgttgctac
     6481 ttgagtgcca aatgcttttt caagtacatt ttttgcatat tctaaaccgc cttctgttat
     6541 tactttttgc gtaagacata gctcgtaaaa atctttgagc gttgattcca ttgtttgggg
     6601 actaaggtgc tgcagcctgg aaatttcata tgttaattgc tcgatttcgt cttcctttaa
     6661 aaatttataa attcttgatg catcttcggc accgagagat ataattattg ccgcagcttt
     6721 ttgtttacct gttagtcttt ccgccatatt attcatcccc ctttaaccag gttctgatta
     6781 gctgtgatac gatttccgga ctggttttcg taaagtcttt aagttgtttc ttaatcgctt
     6841 gctcctctgt ttcttgaacc ttgattccat cttgtatatc agcccatgaa tactcgcttg
     6901 aatctgtatc tgatgttatg gctgcattat ctaaggcctt tttctttttt ctttttctta
     6961 acacaagaat taatattatt aataatattg aaattgcaat taaggctata ccgccgtaaa
     7021 taagcagctc ttttaattga atcctttcaa agatacctgt tgcagccacc ggtactgccg
     7081 gttcttctaa gtcataaaac accatattgc tcaatgcaat atcttcggtt ccgatacctg
     7141 cagcaaatgc tatgagttga ttaatttctc ctgcttcttt atcgctcatg tttgctttgt
     7201 taattactac tgctaccgtc agcttctcaa ttgttcccgg attgtgctca atttgctcct
     7261 ttaactggct taccaggtaa ttaattgtgt ttgaatccct gaaatatata tcatctccgc
     7321 tgactaccac accgggataa gttggtactt ctgcattagt ttcagttcct gcaattcccc
     7381 ctgttgtttc tccgggacca gtaatttctt tttcgctatg agtatcgccg ggtacaccca
     7441 gtcttgtttc ctcgtccggt aaatactgca atatttcgct gatttttttg tcaaaattga
     7501 tagtacattt tgccgaaacc ttgacatttt caggtccgta tattcttgaa agaaattctg
     7561 ttacatcttc ttcagtttct ctttcaaatt ctctttctat actaagtttt aattttattg
     7621 tatttgtttg gagcatttca ttgctaatat tcaaactatt cccgttggta tcaattattg
     7681 caacattttc ttcctttaat cctttaacgc ttttaaccag caactgcatt ataccgtttg
     7741 tctgggaagg agatagggaa tatccgtttt gcaaatttat tttaacagag gcagaagatt
     7801 cctttttatc ggtttcccat gcaaaactct tgtcttgcgg taaatttata gttacaattg
     7861 cttccttaac accgtcaata gtttctattg agctttgcag tctttcttgt aactgaaaaa
     7921 tttcatattg cctcttttca taatctgttg tcatgaaatc aatattttcg gtaaagatat
     7981 cgtaatttat accggttctg ggatggccgg cttgagctaa ttgcattctt aataagcttt
     8041 cttgttcttt aggtatcaat atggttccat ctgtttgata tttatagtca attttgcttt
     8101 cttgcagctg tttcataact tcaaccgatt cagcttcatc catatttcga aataaaacta
     8161 cataatcttt gttattcagt ataaaggtta ttattagtgc accaattacc aatacaagaa
     8221 gtccgcctat tataaggctt tttttccttt tatttagttt tccccaaaac tcagtaacat
     8281 ttttccatat gttttttaat tgttctgaca tttatagctc ctaagatagt atgagaccta
     8341 aacccccata cgcattattt cattgtaagc ttctaaagct ttatttctaa gttgtattac
     8401 caggtctaaa gaaatttgtg ctttttcaac atttatcatt agattgtgca aatcatccgt
     8461 ttcacctatt gataatttgt aaatgtcttt atctacttct gcttctgcat tcttataatt
     8521 ttcaattgca gtatcaaata ttgatttgaa tgcgagttcg cttattttct ctttcggctt
     8581 atttaattca ttaatttgag atgcatttat cggcttaata ttttcaataa acataattac
     8641 ctcccgattt ctaatgcttt aattgccatt tgcttaaccg tgttaagagc agttatgtta
     8701 gcttgatatg ctctgaatgc ttccattata tctatcgttt cttttaatga atcaacatta
     8761 ggcatattaa catatccgtt ttcatccgca tcgggatgtt cgggattgta tactgctttg
     8821 aactcactgt tatcttccat aatttcagca acttccactc cacccggctc atactgattt
     8881 aaatttttca ccatcatatt tttaaagtcg ccggaagttt ttaatacaac catttttctt
     8941 ctgtaaggtc ctccttcttc agtccttgta gtatcaatgt ttgctatatt ttccgaaatt
     9001 acatccatcc ttgccctttg cgcagttaaa ccagacccac ttatattgag cgtttttaaa
     9061 aaagacatat taccctcctt gaaatcgtta tcttatgtat attatcattg tgtatctcat
     9121 gctaagtcta ttaagtttat tttttccctt cggatattac agattttaat ttggaaaaat
     9181 aagaattgat ttctgcgaca gtatacatat actggatttg cgttttagcc agctgcaaat
     9241 tttctttttc taaatccaca ttgttgccgt ctaaacgcat ggataggtta tcattttcct
     9301 taatgataat attaccggat aaaatttgct cgtttatttc agatgtagtt tttccgtatg
     9361 tattttttag attagccgat agaatggttt caaaatcaac tgtcttgctt ttgtatccgg
     9421 gagttgaata gttggctatg ttttccattg ttgtttgttg tctaagccat aaaccatcca
     9481 aacttttctt taacaaattt aaatttacat tgactctcac ttcttgcccc tccaacaaat
     9541 ttatgaaata aagagtgatt tgaaaatatc caaaaaagaa atgtataatt attgacagtt
     9601 accaaaataa ctatgaatta tacatttctt ttgatataaa tcatacatgg caaccggcta
     9661 ccatagacaa gtctttaggt ccaacagctt tgcgtcacta cctttcgata gttttgccct
     9721 ttacacataa gtattaagtt gtataaattt gtaaagtaat cccattataa atgaggttaa
     9781 aacaaaatgc aagccttttt tacaaaattt tacaaaaatc tttatacatt ttcttgattt
     9841 attattcgta taatgccttt atatttatac atcctcatgc gttattttct ggtgcattac
     9901 agcgaataaa aaaatgggta ttgcaaaaac acccataatt gttataaata ttattttttt
     9961 tcgactagca aaattagcat aattataaat tatgcatcaa aatatcaaaa aaatattaca
    10021 tttttttaat tttttattgc ttttaatttg ttttttatat catttaagct tatttcattt
    10081 attttttcag aagcaatttt attaaattca caagtttcag ccaattcttt tctggtaatt
    10141 tttatgtctt gaggagcact gatagcaatt ttaactttat cttgacttat ttctgaaata
    10201 attatctcaa tatcatcacc gataagtatg gattctgata cctttctttt tatcactaac
    10261 atttattacc ctcctcagtt gatactatat tgtatctgag tggatattca ttttgcagta
    10321 taacctgctt agctgttctg ttattaggat taattgcaat cgggcttttt aaattaacca
    10381 cggattcttt aatatcgtct tttataattg caattaccaa atacttcaat tctgccgtgc
    10441 cggttgattt cagctcacat aaatcatctt cgtttaaaat cggattataa tctataaaaa
    10501 tagcatacgg atcaatcaac acaaaagata gttccatatt atcaagtgat tgaagaaaca
    10561 ttatatcatc ctcgggacta tcctttaata taatatattt tttgtaatca tcaaatccgt
    10621 atatgcctgt ggtgaaattt attatatctt cttcaccaac caaaatttct ccaaaatctc
    10681 ttgttttaat tttcattttt tcagacaagg gtatctatct ccttataatt aggattcgca
    10741 ctgggcggca caaaaatcgg ggtacctaca tattttattt ctacgcttgg gtattcactg
    10801 ataataaatt ctatacttcc gggaataaat tttatttgag gtttttcaac attccagtca
    10861 aaatctaatt tatccattgc aaaactcatg gaaattcctc ctgtatccca tgaaatatca
    10921 ggacctgtgg aagggataaa tccaatatta aaactcacat cactgatgaa ctttttcatt
    10981 gcaatttcag gtattggatt atccataata ttgatatcca acataaggtt gccttccctt
    11041 gcataagttg cagttgcttc ataagcagct tgtacacctt ttttggcaac atcactaatc
    11101 aagtgcattg cactttttaa tcctgccgaa tatcttgctt ctacagtatc gatttttatt
    11161 ttggcaggct tcatctgcat ttgaaggccg cccttgtctc ttgatacttc aaaagtggca
    11221 tcggtagttg ccagttcaag gcgagctcta ttaattttca tttcgaatgc cattggaata
    11281 gttttaattt ctaatagcgg ttccatatta ttttctcctt gctatttcat aaaatctata
    11341 aaactatttt gcagaatcct attgcccatt gataatgctg cattataaga atattcctgc
    11401 atcttaaagt ttactattgc ttctgcaggg tcttcaaatt caacttccaa taatttttta
    11461 tttaaattga attgattatc ctcatttctt gtctttaaaa agtccaaata atttgtttta
    11521 gccccaatat cggtaatatg aactaatact tgtttttttt gctcttcaaa ccggtctatg
    11581 tatggtgaaa ttttatctat tgaaaagtcc ggatttctca attcgtcctt tatttttcct
    11641 attaaattat ataggttttc cggattatcg tccgttcctc cataccccat aaactttatg
    11701 cccgacattg acatatcaaa tactgtgtca gtgtttaatt ctccgtcacc gttaaaagtc
    11761 attcctaagc ctaagtctat gttaattgtt tcattcgcta atttatctaa ggcttcctta
    11821 ttggcaggat cattaacatc aagtccctta aataataaat ttccattcat atccaatgta
    11881 aaagggattt cttccttgtt tgttccaccg aaaacatatc tgtcttcaaa ctttgcattg
    11941 agagatgtca gtatggagcg ctgcaaattt tcaagctgct ttgcaataat ttctctgtcc
    12001 tcatcattca ttgttccatt aatacctttt aaatatgagg tgtaaactga ttccatattg
    12061 ttgcttattt ccgtcaaatt tgtttctgca gctgtaagaa atgattcaac atctcgtatg
    12121 ttggtatcgt atatctcggt tgttctatat tctcttctga gcttgtatgc ttttattgca
    12181 gatacagggt cttcagagcc tttaacaaac ttcctgcctg tttcaacctt ctcacttaaa
    12241 taattcattt cataagcaga tttgtttaaa ttttttatat atttatttga catcatactt
    12301 gtagttattc tcatactatc acccctatac tcccattctg tttatcaatg tttccagcat
    12361 ttcatccaag gtggtcataa gccttgcggc tgcattgtat gatttgctgt actgaagtaa
    12421 atttactcct tcttcattca aatccacgga agacatgctg cttcttgcat agtctatatg
    12481 aaattgggtt tctgaatatg tatcaaatga tatttgtaca tcagctgtct gcagatttaa
    12541 ttttgttgtt gtatatgaca aaaattcttg gaatgtacct ttaaacagta tatcatttgt
    12601 agtgcttggg tctctgtcaa catcaaattc attcttagca ttaaatgcat ttatcattcg
    12661 aagtatattg tcaacagctc cggtattatc ctcatctacg ccagctttct ccttaatagt
    12721 gttggttata tatgagtctg ttgcacttgc ccagtcatct gatattttta tgttgccggc
    12781 tcttatgatt tctgtatcag ggtcagatgg atctgcacct cttgctccga atagaggttt
    12841 tgtaatccat gtctgtacat ttccgtctat gtcaaatgaa tccggcttcc tgttcagctc
    12901 attcataact gttgcgaatt tatttgctaa tgtgtctaac atattattat aatattgaat
    12961 acctttaact tctgctgcct tttcatatcc attaaccggg tcaatatcat ccaccgtggc
    13021 aaattcgccc tttccgttaa ggaactttaa gtaaccgtct atttgaccgc cttttataaa
    13081 attggtaata tcgtccgtat ctgttgcacc tgttaattct attttaatta atccattttg
    13141 ctcattaaca gaaattgtgt tgctattatt tctattaacc aattcatatg ttgtcccgct
    13201 taatctgata accaactcgt ctattgtctt gcctccacct atattgagag gctcaatatc
    13261 tacttctata tctatatagt tggaaagctc atctaccagc atatttcttt catcattaag
    13321 ttccaatgcc ggattgccgc ttatattgct ttctttaatt tgctgattta atttggctat
    13381 gttttttgtc aactcattta ctttttcaac agctccattt tccagataat taagttgttg
    13441 attgtatata gtttctatat ctcttgaata atcattgaac atttgagtaa gaatttctgc
    13501 agatgttctg acaacacctt ccattacagg gtctgaagga ctgcttgtca gtgaatgaag
    13561 ctgtgttaaa aggtcggaaa attgcgcatc taatttatcc attgatattt catcaaacac
    13621 tgattccaag tctctcaaag cttctaattg aacctgctcg ctgcctgcct ttgaagtttc
    13681 cattctatat ctcaaatcaa gaaatgcatc tctaaactgg ctcacaccgt ttgcattaac
    13741 tccttgtcct attacaggtc ccggagtgcc atactttaaa ttgcttgtgt taaaagcaac
    13801 agaacttatg tcaagacgct gcctggtgta tcccttagta tttatattcg aaatattttg
    13861 ccccactaca tccatgtggt tttgatttac ttttaatgca cttgttgctg ttttatatcc
    13921 taaaaaagtt gacctgagca tagcttctcc taactttctt ttatatttta tgtgaaatca
    13981 ataaaccttc ggttacattt ttgcttttgt catttgtata tatattttct ttttctccta
    14041 aattgttcat tttattgttt atcctgcgaa gtcttacttc aataacaatc tttaataaat
    14101 cgtttatttt atttaattcc ttaattttat tatttaattc ttcaaattgt tcttgtaatt
    14161 ttattttaga ttcattttcc gccgcatcaa ttatttcttt caaagtcttg ccgcttaatt
    14221 ccaatgaatt tagaagtttt tctctcttat gttcaattcc tttcatttgc agataatata
    14281 cttcttcttg tgacactata ttatctaatg caattacatc atcttctatt actgcttcat
    14341 atttatcata ttctagttcc aaaaacattt tgtaaatttc ggtgtattct tttaataatt
    14401 ggtataaaga atccataata gccccctacc ttaataacct tcttactatt tcttcaacat
    14461 ctatgctata agttttattg tttatgctgt ccttaattct gttaatttta tcagcatttg
    14521 tttcttcgtt aatttgtgat actactttct tcttaataga ttgaaggctg atattttcct
    14581 tatttttatt attaaaatct attacatcat atttattttc tttattgatt tcttcttttt
    14641 tgattagatt agaattaccc ttatagttta tataattatt attgttgata ttattaattt
    14701 tcataacgtc cctcttctat ttattttcat ataatattaa tcgacagaaa tacaaaaaaa
    14761 tttagctaat ttcatcatgc attgctcatt attttttctt catctataac taaaagaacc
    14821 tcttcacttt tatgtccttt ggcaacaccc gttataaagc cgttattaca catttgttta
    14881 atttcttcgg tctcttcata agcaaaaata ttctctacag ataaaacttc atctacaacg
    14941 aggcctataa acgaattgcc cttttccaag actacaacca tagctttgtt ttcttttgaa
    15001 ttattggccg aattgtttcc ggattcatct tcggtattca ataataattt taagtctacc
    15061 aagggaatta cgtttcctct cagatgtatc aatccgataa tatgctccgc tacattaggc
    15121 acaaatgtaa catcatccgg tttgataaca atcgatgtta tatacttact gtttacggtg
    15181 tataaggttt cattaagctt aaatatcagc cagggaatga cctctcgcat ttgcataata
    15241 ttttcattat tcattgtaat ctcccattat ttcatcttta attaattcca aggatccaat
    15301 ttctttttcg ttaagtaact tattaaggtc aattaatata actatttttt tatctaattt
    15361 ggcaattccc gttatataag taataacata attattgccc acctgcggcg gctcggatat
    15421 attttcataa tatatattta taacttcatt gacggaatca acaataaagc cgatgtataa
    15481 gccttcgatg tttgtaacaa ttatacatgt cctttcattg tattcaattt cctgtttttt
    15541 tagccttatc ctgacatcaa ttatgggaac gataatccct cttaaattga taattccttt
    15601 tgcatagctt ggaaattcag ggacttcttc aatctcttgc atccccgtta tttgtacaac
    15661 atgagcaatg ggaattccga atacttgatt ttcaatataa aaagtaagat atttattaac
    15721 ttgagcatct tcttcttcaa tatatgtatg caagctatta aatttgtcca taacttcacc
    15781 tcgtgatatc attctatata agttatcgtc atgtttaata aaaacattag aaaaatttac
    15841 ataataccgg ttaagctatg gggcataaaa aaacagccga tagatttctc tattgactgt
    15901 ttagtatttc tctttttacg agattacatc attcccattc cgccgcccat tggtggcatt
    15961 gcaggttctt cttttttagg ttcgtctgtt actgccgctt ctgttgtcaa taacattgca
    16021 gctacgcttg ctgcattttg aagggctgat cttactacct ttgttgggtc tacgatacct
    16081 gcttcaatca tatttacata tttttcgttt aatgcatcaa atccgacacc tttttcgcta
    16141 tttttgattt tttcaactat tactgagcct tcaagacctg cattgattgc aatttgtctg
    16201 agtggttctt ccaatgcttt ttcgattatc ttagcacctg tctttgcatc tccgtccaat
    16261 ccttctgcta ccttgttaac tttatcgatt gttgttaaaa gtgcaacacc gcctcctgat
    16321 accaagcctt cctcaactgc tgctcttgta gcatttaaag catcttcaat tcttaatttc
    16381 ttttctttca tttcggtttc tgtagctgct ccaacattga taactgccac tccgcctgtt
    16441 aatttagcta atctttccat cagcttttct ctgtcgaatt cagaagttga ttcatcgtgc
    16501 tgagctctaa tttgtctagc tctttcttcg atttcttcct ttgaacctgc accgtttact
    16561 attacggtat tttctttgtc tattttaaca gttccggcat taccgagcat atctaaggtt
    16621 gtttctttta attctaatcc taactcttcg gatattacct ggcctcctgt cagaattgcg
    16681 atgtctctta acatttcttt tcttctgtca ccaaagcccg gagcttttac cgctacacaa
    16741 ttaaaggttc ctcttaattt atttactacc aatgtagcta atgcttcccc ttctacatct
    16801 tctgctataa taagtaatga tttgcctgtt tgaacgattt gttccaaaac gggaagaaca
    16861 tcctgaatgt ttgtaatttt cttgtctgtt attaaaatat acggatcttc taaaacagct
    16921 tccatttttt ctgtattagt taccatatat gccgataagt accctctgtc aaactgcata
    16981 ccttcaacaa cgaccagctc tgtatccata gtctttgatt cttcaacagt tataacgccg
    17041 tccttgccaa cctgatccat tgcttgtgat attaattttc ctatttcttc atctcctgcg
    17101 gaaatagaag caatttgagc aatttcttct gatgtttcaa ctttttttgt tatgttttta
    17161 agttcttcca ctgctgctgc aactgctttt tcgatacctt ttttaagtat catgggatta
    17221 gcgcctgctg caacattttt gagtccttct cttattattg cctgtgccaa taatgttgct
    17281 gtagtagttc cgtcacctgc tacgtcattt gtttttgttg caacttcttt taccagttgt
    17341 gctcccatgt tttcatacgg gtcagaaagt tcaatttctc ttgctattgt aactccgtca
    17401 ttagtaatct gcggtgagcc gaatgatttg tttaaaacaa catttcttcc ctttggccct
    17461 aaggtaactt ttaccgtatc tgctaatgca ttaacgcctc tctccattgc gcggcgtgca
    17521 tcttcatcat atttaattat ttttgccata tttatctctc ctttttattc aataactgcc
    17581 aatacatcag ctaattttat tataattaat tcttcaccgt caattttcac ttcagttcct
    17641 gcaaatttag agtaaataac cttgtctcct actttaattt catctttttt cttttcatcg
    17701 tttgtaattc ccgcaccaac agcaagaact tcagcaacct gcggtttttc ttttgcagag
    17761 ccgggcaata caatgccgct tttggttttt tcttcggctt caatcatttt aattactact
    17821 ctttcgccta atggttttac tgtcattatc aattcctcct atatttaatg ttaatatata
    17881 cgattttttg ttagcactca actccaaaga gtgctaacac ctattcttta tattactcat
    17941 ttgcctttgt gttttcaacc cctatttacc ccaattaaca cccataaaca tattaatata
    18001 taattattcc taccgttttc tttcctttgc tttaaattgc tccatatttc ataacaaaag
    18061 accgtgacaa ggggacgggg gtattgtcat atgacaaggg gacggggcgt tgtcataaaa
    18121 tatattttaa ataatagatg ttttttatgc cgatacgttg tataatgaat ttaccagtat
    18181 aatacatagg agggtttaaa atggctcttg taacatcaca agaaatgttt aagaaagcat
    18241 atgagggcaa atatgctgta ggtgccttca atgtaaacaa catggaaatc atacaaggaa
    18301 tagtggaagc agcaaaacag gaaaatgcac ctcttatact ccaagtttcg gcgggcgcaa
    18361 ggaaatatgc aaatcctatt tatttaagaa agcttgtgga agcagcaatt gaagacacag
    18421 gattagatat ttgtctccac ttagaccatg gtgaagattt tgaaatttgt aagcaatgtg
    18481 ttgatgatgg tttcacctca gttatgattg acggctctaa atatcctttt gaagaaaaca
    18541 tagctcttac gaaaagagtg gttgaatatg ctcactccaa aggggttgta gtagaagctg
    18601 agctgggtaa attggcaggg gtagaggatg cagttaaagt aagaaccaaa gatgctacat
    18661 acacagaccc tgatgaagct gtggaattcg tggaaagaac tggagtagac tcattggcaa
    18721 ttgctatcgg aacaagccat ggtgcttata aattcaaggg cgacccgact ttggactttg
    18781 aaagattgga aactataaca aaccttctcc cgggtttccc cttagtactg catggttcat
    18841 cctcggtacc aaaggagttt gtggatttat gcaataaata cggaggtcaa atcccgggag
    18901 ctgcaggggt gcctgaagaa atgctaagac gtgccgctca attaggggta tgtaaaataa
    18961 atatagatac agatttaaga cttgctctta ccgcatgtat cagacaattt tttgttgaac
    19021 atccttctga attcgacccg agaaaatatt taggacccgg aagaacagcg attaaagata
    19081 tggttgctca
//
LOCUS       DAOPUE010000031        18950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_2952_length_18950_cov_9.167928, whole genome shotgun sequence.
ACCESSION   DAOPUE010000031 DAOPUE010000000
VERSION     DAOPUE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18950
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2952_length_18950_cov_9.167928"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..234)
                     /locus_tag="PKH42_04475"
     tRNA            complement(152..234)
                     /locus_tag="PKH42_04475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Leu,seq:tag)
     gene            complement(264..>1164)
                     /locus_tag="PKH42_04480"
     CDS             complement(264..>1164)
                     /locus_tag="PKH42_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOA19718.1"
                     /translation="RNMISNEDLIRMHLINGLGRKTICKILACIESENIKLNNFSEVL
                     KLLSDMKIKRLKIDEEKIEKEASIIFECCKKNKIKIVGLYDEIYPYKFRLIEDKPLLL
                     FVDGDLELLNQANNIAIVGTRTPSQEGYEISSILAEKLTEEGCCIISGFASGCDEAAH
                     YGSLQANGRTIAVIPSGHINVLKQNRSLYNMIILNKGCIISELPPLAKADNNSFMDRN
                     RLIAAVSEGLIVIEGGLRGGTSHVVKFAKEYNKPVAYTANMCKITGQTLIFKDIDIID
                     SFEKLVKFKSKSCNKILDKAIPQ"
     assembly_gap    1165..1264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1463..1747
                     /locus_tag="PKH42_04485"
     CDS             1463..1747
                     /locus_tag="PKH42_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HOA19719.1"
                     /translation="MILLVRFEDIRLPLLPFLGVDLSEGMGPEYPRILLLEIMLNSIS
                     AMHPEIKPVEIGGIFDSDTRAAVISFQSLYGLPVTGMADAQTFYHHLIIV"
     gene            1833..2348
                     /locus_tag="PKH42_04490"
     CDS             1833..2348
                     /locus_tag="PKH42_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA19720.1"
                     /translation="MNRRGRNIPYVARNKIRKTEAPAELPLLNENQLNEDAVIQTGYV
                     NVSVNTALGALPVEDAVVTLYVTDQEGNQEALYHLVTDISGKVPRMALPVVYNPQNPS
                     ESREYFFSTYNLRIQAIGYYTQNILDIRVFPDTTTNFNVTLIPISQNGDEDEEERTLV
                     IPSSPIDRSNV"
     assembly_gap    2391..2490
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2625..4043
                     /locus_tag="PKH42_04495"
     CDS             2625..4043
                     /locus_tag="PKH42_04495"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOA19721.1"
                     /translation="MSIPEIDVVIPELPLPHVIVPFPLYITVHLGAPDDEEALNVTVP
                     YLEYIKNVASSELYPTWPEEALRANIHAITSFAMNRIFTEWYRSRGYDFDITNTTQYD
                     QAYVHERGIFDTISNIANEIFNVYITREGHIEPLFAAFCDGRITQCDGMYQWGSVELA
                     NQGYTAEEILKYYYGEDITLVESTAAVEITGTYPGEPLSIGDSGIDVFRMQHSLNVIH
                     NNFPLIPAVRITGFFDEETENAVRIFQEAFNLPVTGIVDRDTWYRIRFIYIAVTNIAE
                     LVSAGLTYEELQELYANIVLQGATLPVVVYIEFFLNVLSQKYDAIEPVEIDTFYGPDT
                     DEAVRQFQIIMGLNPTGIVDQQTLNLLFREAYLILTETPVEELELPVLPFIGMELTEG
                     MGLEYPRILLLQIMLNAISAIHPEIRPVEIDGIYGPDTTAAVIIFQNLYGLPVTGIVN
                     EETWNKLNEVYQLSLLERETNT"
     gene            4056..4598
                     /locus_tag="PKH42_04500"
     CDS             4056..4598
                     /locus_tag="PKH42_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOA19722.1"
                     /translation="MVTDLSFNKNLVMVIQNHLNNIKNLYPSLPSVTPTGIYDEATQN
                     AVLIFQKIKGLSPTGFVDVPTLNALVHENHECVKKTQTPYKISFSTRDFVDVKLGDQR
                     DIVYVIKIMINGFCRRYANYNKLEVTNVFDEETEEAVRLFQQRSMLPPSGIVDIKTWN
                     ALVKIYDVCRLYKENSCVRN"
     gene            4813..6684
                     /gene="htpG"
                     /locus_tag="PKH42_04505"
     CDS             4813..6684
                     /gene="htpG"
                     /locus_tag="PKH42_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOA19723.1"
                     /translation="MAKKQFKSESKRLLNLMINSIYTHKEIFLRELISNASDAIDKRY
                     YMSLRDKNIDFNKDDFYIRITPNEEERILRVSDTGIGMSKEELDSNLGIIAKSGSFDF
                     KENNEIKEDVDIIGQFGVGFYSAFMVSEKVTVISKRADSEEAYKWESTGADGYTITPC
                     KKDSVGTDIIMRIKPSTEDEDYDEFLDAYNIKSLVKKYSDFIKYPIKMMMKKRKLKEG
                     TENEYEDYFEDEILNSMVPIWRKNKSELKEEDYHNFYTEKHFGYEKPLKYTHVSAEGV
                     VSYNAILYIPARAPYDFYTKEFEKGLELYSNGVMIMKKCGDLLPDYFGFMQGLVDSPD
                     FSLNISREVLQHDKQLQFIAKKIKEKIKSELLSMLKEDRETYEKFFINFGKVLKYGLY
                     SDWGLNKEFLQDLVMFYSSYEEKLTTLDEYVSRMKEDQKYIYYAAGESVDKLSKLPQA
                     ELIADKGFEILYFTDEIDEFAIKVLLNYKDKEFKNISSSDLDIKSEDDNTDNVEENKE
                     LFAFMKESLGEKVKEVRASKRLKTHPVCLATEGELSIEMEKVLNALPDKPSSIHAEKI
                     LEINTSHQIFEKLKDAFHNDKEKFKKLSNVLFNQALLIEGLTIEDPVQYANDVYELI"
     gene            complement(6984..7832)
                     /locus_tag="PKH42_04510"
     CDS             complement(6984..7832)
                     /locus_tag="PKH42_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019153883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOA19724.1"
                     /translation="MRRNKKSNIKKTLIAVTALLFILFAVLLIKEKLNNDKLPPQEVQ
                     EEENPPNNQNKPEDEEETSTEPEKEPQEEEPKEPEPEQVPEDTEYGIIIQNPEKIDVL
                     VNRKRNLPDTYVPEDLVTLSEVPTVLSNPEVNQLRSAAYEALKELFNAAWEEAGFELC
                     ARSGYRSYNTQSSLYTSYVESYGQQAADKYSAKPGQSEHQTGLAIDITCEALNYKLDT
                     SFAETEEGKWVSENAHRFGFIIRYPKGKEEITGYAYEPWHIRYLGVELAEEIYESGLT
                     LEEYFQ"
     gene            complement(8019..8465)
                     /gene="rimI"
                     /locus_tag="PKH42_04515"
     CDS             complement(8019..8465)
                     /gene="rimI"
                     /locus_tag="PKH42_04515"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOA19725.1"
                     /translation="MGEVIIRGMLPKDIDQVMNIERICFSLPWSRASFENELKNELAY
                     YQVAEVSGKIAAYMGMWKIIDECHITNVAVLPEYRNKGIAGRLIEKMIEICRCSEISF
                     MTLEVRVSNTPAINLYTKYGFFAVGKRPNYYVKPVEDALIMWKQID"
     gene            complement(8475..9164)
                     /gene="tsaB"
                     /locus_tag="PKH42_04520"
     CDS             complement(8475..9164)
                     /gene="tsaB"
                     /locus_tag="PKH42_04520"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOA19726.1"
                     /translation="MKILSIESASVTASCAVSDDEKILGEYTLRHKKTHSEKLMPLIE
                     ELMGELNLKINDIDVISISKGPGSYTGLRIGAAIAKSLAYAADIKIVSIPTMKSLAAN
                     VYDEHKLIVPIMDAKAGRIYTGIYKWDNGECKEVLEQFPCNIDELIQILNEYEEPIIF
                     NGDGSVNYRKRIEDGLNKKAYFAPEVFNYLKASTLAFIGYKMAVQGDIVSANEFNPQY
                     LRLSQAERNRK"
     gene            complement(9145..9612)
                     /gene="tsaE"
                     /locus_tag="PKH42_04525"
     CDS             complement(9145..9612)
                     /gene="tsaE"
                     /locus_tag="PKH42_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOA19727.1"
                     /translation="MKIIANNLKDTEKLGRIIAKCAEKGTVICLDGELGAGKTSLARF
                     IAKELGVKDYIVSPTFTIIKEYEGRLPFYHMDVYRIDSEDDMYDLGYDEYIYSEGVTV
                     IEWSKLIEGILPEERINIEIKRINDTKREINIDGKGFIFEKLVSELSNENTFN"
     gene            10106..11458
                     /gene="murC"
                     /locus_tag="PKH42_04530"
     CDS             10106..11458
                     /gene="murC"
                     /locus_tag="PKH42_04530"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOA19728.1"
                     /translation="MNYNFNHIYFIGIGGISMSALAEIMLEEGIKVSGSDRSDSKIIK
                     KLQTSGATIYLNHDSKHISNDIDLVVYTSAISDDNPELIRARELNIEIMDRAEFLGYV
                     MKNYKNAICVSGTHGKTTTTGMLSSVLIETDLAPTIFLGGEMDSLGGNLLHGSYELLL
                     TESCEYKRNFLKFNPTMEVVLNIEEDHLDYYKDLNDIESAFVEYADKLPPDGYLIVNE
                     SYRHLFDNVKCNIVTFGLDKGAYYARNIKLVPEPSYTLMHGDEEIAEIKLKVYGEHNI
                     LNSLAAAAASLTLGIEPETVVKGLFNFKGTHRRFEYKGKLKGANVFDDYAHHPSEMKA
                     TLKTARSYNDGRIITVFQPHTYTRTIKLLDGFAEALSMSDEVILVDIYAAREINTGVI
                     HSKDIINKMKNYSKDAIYAGSFREAADIVRSMAKKGDIIITMGAGNVVDIADMLIADR
                     "
     gene            complement(11617..12501)
                     /locus_tag="PKH42_04535"
     CDS             complement(11617..12501)
                     /locus_tag="PKH42_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOA19729.1"
                     /translation="MEFKEWFKEKVSIVDEYLGQIIEVKENPQKIIYQAVNYSLLSGG
                     KRLRPVLFLGAYELFRDDLKKALPFACAMEMIHTYSLIHDDLPAMDNDDYRRGKLSNH
                     KKFGEARAILAGDALLNKAFETGLDAAINLKDINAIKALKLIADSSGTEGMIGGQVID
                     IDGENKISSIEDLKYMYSLKTGAIIKSSVTAGATLAGATENEIKLLENYAEKVGIAFQ
                     IMDDILDVTSTQEKLGKLIGSDAANNKTTYLTFKSIDEARKDVEKFTEEAIKSLSLFG
                     NRAIYLIELAKYLTSREN"
     gene            12862..14571
                     /locus_tag="PKH42_04540"
     CDS             12862..14571
                     /locus_tag="PKH42_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA19730.1"
                     /translation="MKMRKIFALFLALLMVALVFAGCTQTSQPSAPQADTQPPSEEEQ
                     QADEAKSEQILRYGASGFNGIFNPIMFDNVYDGYISNIIFEKLITHTPEGEYIPGIAE
                     WKLSEDKLTYTFTLKDGIKFSDGTDLTAEDVAFTYMTIAHPEYNGPMAYAVSNFEGYD
                     EYHEGVTDEFEGIKVIDEKTISFTFKDGRAAPANIECFDYGIMSSDYYAFENWEDFLA
                     LIDKPMGSGIMTLDSWEPKQFILLKKNTNYWDPENGAKIDSLLVSEVPEESILSALQT
                     NQIDFAQISPSAENLEAAKAMDNINIVNYIGNGYTFMCFNTTRPALEDVRVRQALMHA
                     LDRESFIKVQYGEGLAAVGMAPISPASWAFPDLSELNPYSFDMEKAAELMDEAGWLME
                     SDGYRYKDGQKLAVNWLVFTEADWPKTLYEMAADTWKQLGVDLSMELMDGNSVIARTI
                     GPEPGEKDFDIYTLAFTLTIDPDPTGFLFDDDAYMAGGFNASGYKNAQAMDLVRAGKK
                     EFDIEKRAEIYKEWAKIMNEEIPHAIIAYRSEIWGINKRVDGMFIDTYATWDLSLKNV
                     TLK"
     gene            14640..15605
                     /locus_tag="PKH42_04545"
     CDS             14640..15605
                     /locus_tag="PKH42_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19731.1"
                     /translation="MRYYILRRIIQIIPVLIGIIFILFFILEQAPGTPVSYMMHPRMT
                     PEQKAELEEKLGLDIPWYERFVNYIKEASQGNLGYSSTHKKPVTEVIGNYAGPTLLLA
                     LVSLTISIFIGIPAGIISAVKQHTVIDNILTVMSFIGISIPSFFFGLLLLKAFAVDIQ
                     LFPLFGLKDPLLMSDNIFVKIKDIAWHLVLPSIVLGLNSTASFMRYTRSSMLEVIKQD
                     YIRTARAKGLKEKTIIYRHAFRNGIIPLITLLGFWIPLLLSGAIVTESIFALPGLGKI
                     SVEAAMSRNYPLILGINSILAILTLIGSLVADILYALADPRIRYD"
     gene            15616..16500
                     /locus_tag="PKH42_04550"
     CDS             15616..16500
                     /locus_tag="PKH42_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19732.1"
                     /translation="MAKDKTNNNYTGNQSYWGQVLSRLRKNKMAMISLYILIIIIALC
                     IIIPIISPYEIQTTNLSGKDQKPNSRHLLGTDLIGRDIFTRLFYAGRISLGIALATVI
                     IECVIGVVLGSLSGFYGGIIDSIIMRITEMFMSFPFMIICITVVAVFGNSIPNLILVL
                     AILSWPNIARIVRGQILVLREMEYMEACEALGISDLRRIFKHLLPNVSAYVIVYATLG
                     MASVILVETSLSFLGLGVSPPTPTWGNMIQEARNILIIQQKWWYWVPPGTAIFISVMC
                     FNILGDGIRDALDPKMMR"
     gene            16505..17479
                     /locus_tag="PKH42_04555"
     CDS             16505..17479
                     /locus_tag="PKH42_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19733.1"
                     /translation="MDKKLLEVKNLKTYFYSDGVIIKSVDDLSFYVNEGETLGIVGES
                     GCGKSVACLSLARLVETPPGKYEYGEILFKGEDLLRVSDARIRQIRGNDISFIFQEPM
                     TSLNPVFKIGRQISEVLILHRGISKEEAYEESIRMLELVKIPNPERVVNDYPFALSGG
                     MRQRVMIAMALACKPGLLIADEPTSNLDVTIQAQVLDLMNELKEKINTSIIFITHDLD
                     VIAEMSDRVMVMYAGKVVEEASTNEILKNPRHPYTVGLINSKPNMAKEGTRLNVIPGN
                     VPDLSNLPPGCSFHPRCDKAMDICKRVIPDEKLLYEGRHRISCWLYAD"
     gene            17490..18464
                     /locus_tag="PKH42_04560"
     CDS             17490..18464
                     /locus_tag="PKH42_04560"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOA19734.1"
                     /translation="MSEYLITVNNLKKYFPIKKGLVKRTSGNVKAVDGVSFNIQRGQI
                     LGIVGESGCGKSTMGRTILRLLPPTGGEVFYEGKNVFNMSKREMNKMRTKMQIIFQDP
                     YSSLNPRLPVYDIVGEAMLQHGIVKNKKEMKEKVIEIIGKCGLSPEQADRYPHQFSGG
                     QRQRICIARALAVNPDFMVCDEAVSALDVSIQSQIINLLKDAQEDYGLTYMFISHDLS
                     IVKFISDEIAVMYLGQIIEKGSKKQIFSNPLHPYTEALLSAVPSLKQNKRRIVLNGDI
                     PSLVNPPKGCRFYTRCPYVKDICKEEIPEYREINTGHFAMCHKVNGVF"
     gene            18464..18697
                     /locus_tag="PKH42_04565"
     CDS             18464..18697
                     /locus_tag="PKH42_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19735.1"
                     /translation="MFLKKNRVDEDLERIREANLPNKEEIKMNAKHSDDLSLEKGDLL
                     AMVLAVISLILPYVIAFLGAIGVVIFFMYLFFS"
     gene            complement(18784..>18950)
                     /locus_tag="PKH42_04570"
     CDS             complement(18784..>18950)
                     /locus_tag="PKH42_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOA19736.1"
                     /translation="KGFGFITKDDGNDVFVHFSAIQGDGFKTLEEGQRVSFDVTQGNR
                     GDQAANVQRI"
BASE COUNT         6063 a         3319 c         3498 g         5870 t
ORIGIN      
        1 aataaacact taactgattt agaccgttta caaattcaac atggtctttt acatcataat
       61 tccattaaat tctaaactat tatcttatct ttttcaaaaa ttaatctata tgtgcaaaat
      121 aaaagctccg ttcatatcgg agctattgtt ttggtgcgga tgaagggact cgaaccccca
      181 cgctgctgcg ctagatccta agtctagtgc gtctgccaat tccgccacat ccgcttaact
      241 gttgagcagc aaaactgatt atactactga ggaattgctt tgtcaaggat tttattgcat
      301 gatttgcttt taaattttac cagtttttca aagctgtcta ttatatctat atctttaaaa
      361 atcaaggttt gtcccgttat tttgcacata tttgcagtat atgcaaccgg cttgttgtat
      421 tcttttgcaa atttaaccac atgtgatgta ccgcccctca agcccccttc tattactatc
      481 aggccctctg aaacagctgc tatcagcctg tttctgtcca tgaaagaatt gttgtcagcc
      541 tttgcaagcg gaggcagttc agatataata caacctttgt ttaaaataat catattatac
      601 aaggacctgt tttgttttaa aacattgata tggcctgaag gtattactgc aatagtcctt
      661 ccatttgcct gaaggcttcc gtagtgagct gcttcatcac atcctgatgc aaaaccgcta
      721 atgatacaac aaccttcttc agttaatttt tcagctaata tcgagcttat ttcatatcct
      781 tcctgagatg gagttcttgt gcctactata gctatgttgt ttgcctgatt taaaagttcc
      841 aagtctccgt caacaaacaa caacaaaggt ttatcttcta ttaatctaaa tttgtaggga
      901 tatatttcat catataaacc aacaattttt attttatttt ttttgcagca ttcaaatatt
      961 attgatgctt ctttttcaat tttttcttca tcaattttta atcttttgat tttcatatcg
     1021 cttaacagtt tcaaaacttc tgaaaaattg ttaagcttta tattttctga ttcaatgcat
     1081 gctaaaattt tgcagatagt ttttctgcct aacccgttaa tcaggtgcat cctgattaag
     1141 tcttcattgc ttatcatatt tctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnaacaac atattattgt gttgtatttt agcaaaatga aatgagtgtt tttacatttt
     1321 accacaaatt tttacaaaat agaacacttc tttttattca gaagagttaa gaaaatttaa
     1381 taaatttatg ttataaacat atatttaaat ggatataaga ttttcttaat ttgaggaaaa
     1441 gttttattta tatgaaagca aggtgatatt attagtacgt ttcgaagata taagacttcc
     1501 cttgcttccc tttttgggag tagatttatc cgagggtatg ggaccggaat acccgagaat
     1561 actccttctt gaaataatgt taaacagcat ttctgcaatg catcccgaaa taaagcctgt
     1621 tgaaataggc ggcatatttg attcagacac aagagctgca gttatttcct tccaaagttt
     1681 gtatggtctg ccggttacgg gtatggcaga tgctcaaacc ttctatcatc acctcataat
     1741 agtttagttg tgtacatata tattaagtaa gcaaattctt attaattacc atgtaccaat
     1801 attaccgtta tgcaatagaa aggtgtgatt ttatgaatag aagaggcaga aatattccgt
     1861 atgtggccag aaataaaata agaaaaactg aagcacctgc tgaacttcca ttattaaacg
     1921 aaaatcagct taatgaagac gctgttatac aaacaggtta cgtaaatgta tccgtaaaca
     1981 cagccttagg tgccttgccg gtagaagatg ctgttgttac attgtatgta acagaccaag
     2041 aaggaaatca agaagcactg tatcatctcg ttaccgatat aagcggaaaa gttccgagaa
     2101 tggctctccc ggttgtatac aaccctcaaa acccatcaga aagccgcgaa tatttttttt
     2161 ctacatataa tttaagaata caggcaattg gttattatac ccaaaatatt cttgacataa
     2221 gagttttccc cgatacgacg acaaatttta atgtaactct cataccgata agtcaaaatg
     2281 gtgatgagga tgaagaagaa cgaaccttag tgataccgtc aagcccaatt gatagatcaa
     2341 atgtgtgagg tgtataaatg agtgtaacaa tgattcagca aacatatccg nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agccaaccgg aattgtaaat gaagaaacat
     2521 ggaacaaatt gataagtgtt tatcgagagc ttagatttgg tgatacaatg ccggctcctt
     2581 ctaatacgaa tggcggttaa aatagttgaa agcaaggtga tagtatgagt attcctgaaa
     2641 tagatgttgt tatccctgag ttacccctcc cgcatgtcat tgtgcctttt cctctttata
     2701 taactgtgca tttaggagct cccgatgatg aagaagctct taatgtaaca gtaccgtatt
     2761 tggaatatat aaaaaatgta gcctccagtg aattatatcc cacatggccg gaagaagctt
     2821 taagggcaaa tattcatgct attacttctt ttgcaatgaa taggatattc acggaatggt
     2881 acaggtcaag aggctatgat tttgatatta caaatacaac tcaatacgac caggcttatg
     2941 tgcatgagcg aggtattttt gacactattt ccaacattgc aaatgaaatc tttaatgtgt
     3001 atattacgag agaaggtcat atcgaaccat tatttgcagc cttctgcgac ggaagaatca
     3061 ctcagtgcga cggcatgtat caatggggaa gtgttgaatt ggcaaatcaa ggatatacag
     3121 ctgaagaaat tcttaaatac tactatggcg aagacataac attagttgaa agcactgccg
     3181 cagtagagat tacaggaacc tatccgggtg agcctctctc aataggagat tcaggaatag
     3241 atgtttttag aatgcagcat tcgctaaatg ttatacacaa caatttccct ttaattccgg
     3301 ctgtaagaat aacaggattt tttgacgaag aaacggaaaa tgccgtaaga atttttcaag
     3361 aagcattcaa tttgcctgtt accggaattg tggatagaga tacatggtac cgaatacgct
     3421 ttatttatat tgctgttaca aacatagccg agttagtatc cgcaggactt acctatgaag
     3481 aacttcaaga actctatgca aatattgtat tgcagggagc cactttacct gtagtggtat
     3541 atatagaatt ttttttaaac gttttatccc aaaaatacga tgcaatagag cctgttgaaa
     3601 tagacacgtt ctacggtcct gatactgatg aagcagtaag acaatttcaa attattatgg
     3661 ggttgaatcc taccggaata gtagaccaac aaacattgaa tttgctgttt cgggaggcat
     3721 atttaatcct tactgaaact cctgtagagg aattagaact gcccgttctt cctttcattg
     3781 gaatggaatt aaccgaaggt atggggcttg aatatccgag aattctgctt cttcaaatta
     3841 tgctgaatgc aatttctgcc atacatcccg aaataagacc tgttgagatt gatggcatat
     3901 atggtcctga cacaactgct gcagttatta tcttccaaaa tttatatggg ctgcctgtta
     3961 ccggcattgt taatgaagaa acatggaata aattaaacga agtatatcag ttgtctcttc
     4021 ttgaaagaga aactaataca taaaaaggag atattatggt tactgattta agctttaaca
     4081 aaaatctggt gatggtaatt caaaaccatc ttaataacat aaaaaatcta tatccttcac
     4141 tgccttctgt taccccaacg ggaatatatg atgaagcaac tcaaaatgca gtccttattt
     4201 ttcaaaaaat caagggactc tctcctaccg gtttcgtgga tgttccaaca ttgaatgcat
     4261 tagtacatga aaaccacgaa tgtgtaaaaa aaactcaaac gccctacaaa atttcgttca
     4321 gcacccgtga ctttgttgac gtgaaactag gggaccaaag agatatagta tatgtgataa
     4381 aaattatgat taacggattt tgcagaagat atgccaatta taataaatta gaagttacaa
     4441 acgtatttga tgaagaaacc gaagaagcag taagactttt tcagcaaagg agtatgctcc
     4501 ccccaagcgg aatagtcgat attaaaacat ggaatgcctt agttaaaata tatgatgttt
     4561 gcagattata caaagaaaac agttgtgtca gaaactgata aacgtcagta gatgagattc
     4621 ttcgtgctgg ctctcagaat gacaggcatg tcatcctggg catagcgaag gatctcattt
     4681 tttatttagc ccattgacaa tctatgtagt tttgataaaa tatggttagc actcgactgc
     4741 tccgagtgct aatacaatgt aatacaaaaa attaaaaaat agggttagat cattttaaga
     4801 aaggaagata aaatggctaa aaaacaattt aaatcagaat caaagcgact tttgaactta
     4861 atgattaatt ccatttacac tcacaaggaa atatttctga gagagcttat ttcaaatgcg
     4921 agtgatgcga ttgacaagag atactatatg tctttaagag acaaaaatat tgattttaac
     4981 aaagatgact tttacataag aattacccca aatgaagagg aaagaatttt aagggtatct
     5041 gacacgggaa tcggcatgtc aaaggaagag ctcgattcaa atcttggtat catagcaaaa
     5101 agcggctctt ttgattttaa agagaataat gaaataaaag aagatgtgga tataatcgga
     5161 caattcggag tcgggtttta ttctgcattt atggtttcag aaaaggtaac ggttataagc
     5221 aaaagagcag attccgagga agcatataaa tgggagtcaa cgggagcaga cggatataca
     5281 atcacaccat gcaaaaaaga tagtgtcgga acggatataa tcatgagaat caaacctagc
     5341 actgaagatg aagattatga tgagttcctt gatgcttaca acataaaatc actggttaag
     5401 aaatattccg atttcattaa atatcccatc aagatgatga tgaaaaaaag aaagctaaaa
     5461 gaaggaacgg aaaatgaata tgaagattat tttgaagatg aaattttaaa cagcatggtc
     5521 cccatatgga ggaaaaacaa aagcgagctt aaggaagaag attatcataa cttctatacg
     5581 gaaaaacatt tcggctatga aaaacctttg aaatatactc atgtaagcgc tgaaggagta
     5641 gtaagctaca atgcaatatt atatattcca gcaagagcac cttatgattt ctatacaaag
     5701 gaattcgaaa aaggccttga gctttattca aacggtgtaa tgattatgaa aaaatgcggt
     5761 gatttattgc ccgattattt cggttttatg cagggattgg ttgattcgcc cgatttttct
     5821 ctgaatattt caagagaagt attgcagcat gacaaacagc ttcaatttat agcaaagaaa
     5881 atcaaggaaa aaataaaaag cgaactcttg tctatgctga aggaagatag agaaacttat
     5941 gaaaaattct ttataaattt tgggaaagtt ttaaaatacg gcttatacag cgattggggc
     6001 ttaaacaagg aatttcttca ggatttagta atgttttatt catcttatga agaaaaacta
     6061 acaacccttg atgaatatgt gagtcgaatg aaggaagacc aaaaatacat atattatgcg
     6121 gccggagaaa gtgttgataa attatcaaaa cttcctcagg cggaactcat cgctgacaag
     6181 ggatttgaaa ttctctattt tacagatgaa atagatgaat ttgcaataaa agttttatta
     6241 aactacaaag ataaagagtt taagaatata tcaagctccg acctggatat taaatcagaa
     6301 gatgacaata ccgacaatgt tgaagaaaat aaagagttgt ttgctttcat gaaggaatca
     6361 ctgggtgaaa aagttaaaga ggtgagagct tcaaaaagac ttaaaactca tcccgtgtgc
     6421 ctagctaccg aaggagaatt atcgatagaa atggaaaagg tgttaaatgc actgcctgat
     6481 aaaccttcaa gcatccatgc ggagaaaatt cttgaaatta acaccagcca tcaaattttt
     6541 gaaaaattaa aagatgcatt tcataatgat aaagaaaaat ttaaaaagct ttcaaacgta
     6601 ttatttaatc aagcattgct tattgaaggg cttacaatcg aagaccccgt ccaatatgca
     6661 aatgatgtat acgagctgat ataaaaaggc catggaatca tgtggctcag gacactaaaa
     6721 aaaataaaaa tatccgctca cggtttcggc cacgaataat tctaggagct cattccggct
     6781 aaaaatccta cagcttgcga actcgctctg ctcaaacaac gcaagctgtt taacggattt
     6841 ttaacctgca ttcgctaaga attataatcg tgtcctgcaa atgtttgctt gatattttta
     6901 tttttcttag aaataagcga aggatttcat aataatgggt tataactttc catcaaataa
     6961 aaccacgggg attccgtggc tttttattga aaatattctt ccaatgtgag tcccgactca
     7021 tatatttctt ctgccagctc aacacctaag tatcttatat gccatggctc atatgcataa
     7081 cccgtaattt cttccttccc cttaggatat ctgataataa atccgaaacg gtgagcgttt
     7141 tctgaaaccc acttgccttc ttctgtttcg gcaaatgaag tatccaattt gtaatttagg
     7201 gcttcacatg taatatctat tgcaagacct gtctggtgtt cgctttgacc cggtttggca
     7261 ctgtatttat ctgccgcttg ctgaccgtag ctttcaacat atgaagtata caatgatgat
     7321 tgcgtattat atgacctgta gcctgagcgt gcacacagtt caaaaccggc ttcttcccat
     7381 gcagcattga acaattcctt caatgcctca taggcggcac ttcttaattg atttacttcc
     7441 gggtttgaaa gcactgtagg tacttcgcta agcgttacta aatcttcagg cacataggta
     7501 tcaggcagat ttctttttct atttaccaga acatctattt tttcaggatt ttgaattatt
     7561 atgccatatt cagtatcttc aggcacctgt tccggttcag gctcttttgg ctcttcttcc
     7621 tggggttctt tttccggttc agttgaagtt tcttcctcat cctcgggttt attttgattg
     7681 tttggaggat tctcctcctc ctgaacctct tgaggaggta atttatcatt atttaatttt
     7741 tcctttatca gtaatactgc aaataaaata aagagcaaag cagtaacagc aattaatgtc
     7801 ttttttatat tacttttctt atttcttctc attgttttct cctatgttaa tgtcattccg
     7861 gtcatagtca cttgcttaac gaatgataaa cagttatatt ttgggatgcc atcctgagag
     7921 taagcgaaag atctcatgag attcttcgag ctaaagctct cagaatgaca aaaatgttat
     7981 tcatataaat gacaggcttc tgcttagaat gtttgtcatc agtctatttg tttccacatt
     8041 atcaaggcat cctcaacagg ttttacataa tagtttggtc ttttccctac agcaaaaaac
     8101 ccatactttg tatacaaatt aatggccggt gtattagata ctctaacttc aagcgtcata
     8161 aaagaaattt ccgagcatct gcatatttca atcattttct caatgagcct gcctgctatt
     8221 cccttgtttc tgtattcagg caaaactgca acgttggtta tgtggcattc atcaataatt
     8281 ttccacatcc ccatataagc tgcaattttt cctgatactt cggcgacctg ataatatgcc
     8341 aattcatttt ttaattcatt ttcaaaggat gccctagacc atggcaatga aaaacaaatt
     8401 ctttcaatat tcattacttg gtctatatcc ttaggaagca ttcccctaat aataacttcc
     8461 cccatataca cctcttactt tctgtttctt tctgcttgag ataatcgcaa atattgcgga
     8521 ttaaattcat ttgccgagac gatatccccc tgaaccgcca ttttatagcc aataaaggct
     8581 agtgttgatg ctttaagata attaaaaact tcaggtgcaa aatatgcttt tttgtttaaa
     8641 ccatcctcaa ttctttttct gtaatttacg gaaccgtcac cgttaaaaat tataggctct
     8701 tcatattcat tcagtatttg aatcaattca tcgatattgc atggaaattg ttctaatact
     8761 tccttgcatt ctccattatc ccatttatat atgccggtat atatcctgcc tgcctttgca
     8821 tccattatag gtacaataag tttgtgctca tcgtagacat tggctgctag tgatttcatg
     8881 gttggaatgc ttacaatttt aatatctgca gcatatgcca agctttttgc aattgcagcc
     8941 cctattctta accctgtata ggagccggga cctttagaaa ttgaaataac atcaatatcg
     9001 ttaattttaa ggtttaattc tcccatcaat tcttcgatta agggcataag tttttccgaa
     9061 tgagtttttt tatgtcttaa ggtgtattct cccagaattt tttcatcatc agatactgca
     9121 caggaagctg ttacagatgc tgattcaatt gaaagtattt tcattgctta actccgatac
     9181 cagtttctca aaaataaatc cttttccatc aatgtttatt tctctttttg tatcattaat
     9241 tcttttaatt tcaatattta ttctttcttc gggcagtatc ccttcgatta gcttagacca
     9301 ttctattacg gtaactccct cagaatatat atattcatcg tagcccaaat catacatatc
     9361 atcttccgag tcaatgcggt acacatccat atgatagaaa ggaagccgcc cttcatattc
     9421 tttaataatt gtaaatgtag ggctgacaat atagtcttta actcctaatt cctttgcaat
     9481 aaaacgtgca agagaagttt tgcctgctcc taattctcca tccaggcaaa ttactgttcc
     9541 tttttcagcg cattttgcta ttattcttcc cagcttttct gtatctttta aattgtttgc
     9601 tattattttc atatgaatcc taactttata attttactac taaatatttt tcattacaat
     9661 aaaaaactta tagaacgtca tcgctatgaa taagctacgt gtcattctga gagctagccc
     9721 gaagaatctc atgagatcct tcgctttcgc tcaggatgac attgccctgg aatcctatac
     9781 cacactcggg atgacaattt tgttctaatc ataacattgc tattatacat ttatatattt
     9841 taaaaatcaa gaaataaaga tgccctaacc ctatttttca gatgccctaa ccctattttc
     9901 ccgaccgcga tgcccttatc ctaaatagag atagccatct gtgatgatcg aagaaaattt
     9961 tattgaacaa tgtttttact catgctataa tactatgagt atttatccat ataataatca
    10021 aaatcaacaa attttattac aactgtgtta attgcaaaat aattgaataa tattaaacaa
    10081 aataatgaaa cccggggaga ctgcaatgaa ttataacttc aatcatatat attttatcgg
    10141 aattggcgga ataagcatga gtgccttagc tgaaataatg cttgaggaag gaatcaaggt
    10201 atcgggctct gacagaagcg attccaaaat aataaagaag cttcaaactt caggagcaac
    10261 aatttactta aaccatgaca gcaaacacat aagtaatgat attgacttgg ttgtatatac
    10321 atctgcaatt tcggatgaca atcctgagct tatacgagct agggaattaa atattgaaat
    10381 aatggacaga gctgaatttc taggatatgt gatgaaaaat tacaaaaatg caatatgcgt
    10441 ttccggaact cacggcaaaa caactaccac cggaatgctt tcatcggttt tgattgaaac
    10501 tgatttagcc cccaccattt ttttgggcgg tgaaatggat tccttgggag gaaacttgct
    10561 tcacggctca tatgaactcc tgctgactga atcttgtgaa tataaaagaa actttctgaa
    10621 attcaacccc actatggagg tagtactgaa tattgaagaa gaccatctgg attattacaa
    10681 agatttaaat gatattgaaa gtgcatttgt tgaatatgca gataaactgc ctcctgacgg
    10741 gtacttgatt gtcaatgaat catacaggca tttgttcgat aatgtaaaat gcaatatagt
    10801 aaccttcggt cttgataaag gtgcttatta tgcacgaaat ataaagcttg tcccggagcc
    10861 ttcatatact cttatgcacg gtgatgagga aatagctgaa attaaattga aagtatatgg
    10921 cgagcataat attttaaatt ccctggcagc ggctgctgca agcctgacct taggaattga
    10981 acctgaaact gttgttaagg gtttgtttaa ttttaaagga acccacagaa gatttgaata
    11041 taagggaaaa ctcaagggag caaatgtttt tgacgattat gcacatcatc cttcagaaat
    11101 gaaagcaact ttaaaaacag cccgctccta taacgatggt agaataatca ctgttttcca
    11161 gccccatacc tacacgagaa ccataaagct tctagacgga tttgccgaag ctttgtcaat
    11221 gtccgatgag gtaattcttg tagatattta tgctgcaaga gaaattaaca cgggtgtaat
    11281 acattcaaag gatattataa acaaaatgaa gaattattca aaagatgcaa tctatgcggg
    11341 aagcttccgg gaagctgcgg atatagtaag gtcaatggca aaaaaaggtg atataattat
    11401 caccatgggt gccggaaatg tggttgatat agctgatatg ctgattgcag atagataagt
    11461 gtttcatcct gagcgtaagc gaaggatctc gggttttata agatgagatt cttcgagcta
    11521 aagctctcag aatgacacgt agcttattca tagcaatgac gagagctgtg ccctcggttg
    11581 acagggcgtc atcctgagcg taagcgaagg atctcattag ttttctctgc ttgtgagata
    11641 ttttgccaat tcaatgagat agattgctct attgccgaat aggctcaagc ttttaattgc
    11701 ttcttccgtg aatttttcca cgtcctttct tgcttcatct atacttttaa atgtaagata
    11761 tgttgtttta ttgtttgctg catcacttcc gattaacttt cccagttttt cctgagtact
    11821 tgttacatcc aaaatatcat ccattatttg aaatgcaatg cctacttttt cggcgtaatt
    11881 ttccaatagt tttatttcgt tttctgttgc tcccgctaat gttgcgcctg ccgttacgct
    11941 gcttttaatt atagctcccg tttttaatga atacatatat ttcaggtctt cgatggagct
    12001 tattttattt tccccatcta tgtctattac ctgccctcct atcattcctt ccgtacctga
    12061 ggagtcggca attaatttca gagcctttat tgcatttatg tcctttaaat taattgcagc
    12121 atctaaacct gtttcaaagg ctttgttaag gagtgcatca ccggcaagta ttgctcttgc
    12181 ctcaccaaac tttttatgat ttgataattt gcctcttcta taatcatcat tgtccatggc
    12241 aggcaaatca tcgtgaatca atgaatatgt atgaatcatt tccattgcgc aggcaaatgg
    12301 aagggctttt tttaaatcat ctctgaaaag ctcataggct cctagaaaaa gcacgggtct
    12361 gagtctcttc cctcccgaca gaaggctgta attaactgct tggtatatta ttttttgagg
    12421 gttttccttt acttcaataa tttgccccag atattcatct acaatactta ctttttcttt
    12481 aaaccactct ttaaattcca tattaacatt tcctttgctc tgaattcttt tatacaatcc
    12541 tatgctacac ccgggatgcc gcaagctgtg acctcggatg agctgtcata caggtgtaga
    12601 atcttaggtt tttttaatta tatcataaat aagtaaattg taaaactatt ctgaaagcgc
    12661 agctcgaaag atacctaacc gtaaatgagc gtaacatggg taaagcctgc cttctccaat
    12721 caatattaaa ttgactatgg tttgtgcgtc gtatataata tatctaggtt atggtaacgt
    12781 atccataaac ttgtaaattt tttatatctg cactaagctg atataataga atcaattcaa
    12841 aaataatcta gaggagcttt tatgaaaatg agaaaaatat ttgcattgtt tttagcatta
    12901 ttgatggttg cccttgtttt tgcaggctgt actcagacaa gccaaccttc ggcgccgcag
    12961 gctgacacac aaccgccgtc tgaagaagag caacaggctg atgaagcaaa gagtgaacaa
    13021 atattaaggt atggcgcatc agggtttaac ggtatattta atcccattat gtttgacaat
    13081 gtatatgatg gttatatttc aaacataata tttgaaaagt taataacgca cactcctgaa
    13141 ggtgaatata tccccggaat tgctgaatgg aaattatcgg aggataaatt aacctacaca
    13201 tttacattaa aagatggaat caaattttct gatggcacgg atttaactgc agaagacgtg
    13261 gcttttacat atatgactat tgcccaccct gaatacaacg gaccaatggc atatgcagta
    13321 agcaattttg aaggttatga cgaatatcac gagggagtga cagatgaatt tgaaggtatt
    13381 aaagttattg acgaaaaaac catatcattt acttttaaag atggtagggc tgcacctgca
    13441 aatattgaat gcttcgacta tggtattatg tcttcagact attatgcatt tgaaaattgg
    13501 gaagatttct tagcattaat tgataaacca atgggctcag gcataatgac actggacagc
    13561 tgggaaccga aacaatttat tttattaaag aaaaatacaa attattggga ccctgaaaac
    13621 ggggctaaaa ttgacagtct attggtaagt gaagttcctg aggaaagcat attatcggct
    13681 ttacagacaa atcaaataga tttcgctcaa atttcaccga gtgcagaaaa cttagaagca
    13741 gcaaaggcta tggataatat caacatcgta aactatattg gaaacggcta tacatttatg
    13801 tgctttaaca caacaagacc tgccttagag gatgtacgag tcagacaagc tcttatgcat
    13861 gcacttgaca gagaatcatt tataaaggtt caatatggtg aaggtttggc agcagtcggt
    13921 atggctccta tatctccggc atcatgggca ttccctgatt tatcagagtt aaatccatat
    13981 tcattcgata tggagaaagc tgcagagctt atggatgaag cgggatggtt aatggaatca
    14041 gacggataca gatataagga tgggcagaaa cttgctgtta attggcttgt tttcacagag
    14101 gctgattggc cgaaaacatt atacgaaatg gctgctgata catggaaaca attaggtgtt
    14161 gacctttcta tggaattaat ggacggcaat agcgtcatag ctcgtacaat aggtcccgag
    14221 ccgggtgaaa aagactttga tatttacaca ttggccttta cattgacaat agacccggac
    14281 cccacaggat ttttgtttga tgatgatgct tatatggcag gcggatttaa cgcatcgggt
    14341 tataagaatg cccaagcaat ggaccttgta agagcaggaa agaaagaatt cgacatagaa
    14401 aagcgtgccg aaatttataa agaatgggct aaaataatga atgaagaaat tcctcacgca
    14461 atcatagcat acagaagtga aatttgggga ataaacaaaa gggtagatgg aatgtttatt
    14521 gatacctatg caacatggga cttatctttg aagaacgtta ctttaaaata acaaaggggc
    14581 aggcagtcct gccctaattt attgacctta ggctatatca ttataaaaga ggcggtatta
    14641 tgagatatta tattttgagg cgcataattc aaataattcc tgtactcata ggaattatat
    14701 ttatattatt ttttatcctc gaacaagcac cgggtactcc ggtttcatat atgatgcatc
    14761 cgagaatgac tcctgagcaa aaagccgaac tggaggaaaa attaggcttg gacattccat
    14821 ggtatgaaag attcgtcaac tatataaagg aggcatctca aggtaattta ggctattcaa
    14881 gcacccacaa gaagcctgta accgaagtta tcggaaatta tgcgggacct acactgctgc
    14941 ttgccttagt ctctttaaca atttctattt ttatcggtat tcccgccggt ataataagtg
    15001 ctgtaaaaca acataccgta atagacaata tattaacggt gatgtctttt atcggcataa
    15061 gcataccgtc gtttttcttt ggcctcttgc tgttaaaagc ttttgccgta gacatacagc
    15121 tctttccgct gtttgggctg aaagacccct tgttaatgtc tgacaatatt tttgttaaaa
    15181 taaaagatat tgcctggcat ttagtattgc cgagtattgt cttaggctta aactcaactg
    15241 caagctttat gcgttacacc cgctctagca tgcttgaagt tattaagcag gattatatta
    15301 ggactgcaag agccaagggt ctgaaagaaa aaaccattat ttacagacat gcatttagaa
    15361 acggaataat cccccttatt acattgcttg gtttctggat acctttactt ttatcgggag
    15421 cgatagtaac ggaaagtatt tttgcattgc ccggacttgg aaaaatatct gtcgaagcag
    15481 caatgtcaag aaactatccc ctcattctcg gtatcaattc aatacttgca attcttacac
    15541 tgataggatc tttggttgca gacatactgt atgctcttgc agacccgaga ataagatacg
    15601 attaggcagg tacatatggc taaggataaa acaaataata attataccgg aaatcaatcc
    15661 tattggggac aagtactctc aagactaaga aaaaacaaaa tggcgatgat aagtctttac
    15721 attcttataa taataattgc cctatgcatt attattccaa ttatatcacc ctatgaaatt
    15781 caaacaacga atctctcagg caaagatcaa aaacctaatt cccggcacct tttaggcacg
    15841 gatttaattg gaagagatat ttttaccaga ctgttttatg caggaagaat atcacttggc
    15901 attgcacttg caacggttat aattgaatgt gtaataggtg ttgttttggg cagtttatcc
    15961 ggcttttacg gaggaataat tgattcgata ataatgcgta ttaccgaaat gtttatgtca
    16021 tttcctttta tgataatatg cattaccgtt gtagcagtat tcgggaacag tataccgaat
    16081 ctaattttag tactggctat attgagttgg ccaaacatag cacgtattgt aagaggacaa
    16141 atacttgttc ttagagaaat ggaatatatg gaggcctgcg aagctcttgg catcagcgac
    16201 ctaagaagaa tatttaaaca ccttttgcct aatgtatcag cctatgtaat tgtttatgca
    16261 acacttggga tggcaagtgt aatcttagtg gagacttctt taagcttttt aggtctgggt
    16321 gtttctcctc ctactccaac ctggggaaac atgattcaag aagcaaggaa tatactaata
    16381 attcaacaga agtggtggta ctgggttcct ccgggaacag ctatattcat atctgttatg
    16441 tgctttaaca tattgggcga cggcatacgt gatgccttag accccaagat gatgaggtaa
    16501 ttttatggat aaaaaattac tggaagttaa aaacctgaaa acatattttt attcagacgg
    16561 agttattata aagtcagttg acgatttaag cttttacgtt aatgaaggag aaactttggg
    16621 tatagtaggc gaatcgggat gcgggaaaag cgtagcctgt ctgtctttgg cacgcttggt
    16681 ggaaacaccg ccggggaaat atgaatacgg agaaatactt tttaagggtg aagatttgct
    16741 tagggtttca gatgcaagaa taagacaaat aagaggaaat gatatatctt ttatttttca
    16801 ggagcccatg acttcattaa atcctgtatt taaaataggg aggcaaatta gcgaggtcct
    16861 gattttacac cggggaatat caaaagaaga agcttatgaa gaaagtattc gaatgctgga
    16921 gcttgtaaaa attccaaatc ctgaaagggt ggttaatgac tatccctttg cattatcggg
    16981 aggaatgagg caaagagtaa tgattgcaat ggcacttgca tgtaagcccg gtctcttgat
    17041 tgctgatgag cctacaagca accttgatgt tacaattcag gcgcaggttt tggatttaat
    17101 gaatgagctt aaagaaaaaa ttaatacttc aattatattt ataactcacg accttgatgt
    17161 aattgcagaa atgagcgaca gagtaatggt tatgtacgca ggtaaggttg tagaagaagc
    17221 ttcaacaaac gaaatactta aaaacccgag acacccgtat acagtcgggc ttataaactc
    17281 aaaaccgaat atggcaaaag aaggtaccag acttaatgta atacccggta atgtacctga
    17341 tttaagcaat cttccgccgg gatgttcctt ccatccgaga tgcgataaag caatggatat
    17401 atgcaaaagg gttattcccg atgaaaaatt attatatgaa ggacggcaca gaatttcatg
    17461 ctggctttac gccgattagg aagaagtaga tgagtgaata tttgattacc gtaaataatt
    17521 taaaaaagta ttttcccata aaaaaaggac ttgttaagcg tacaagcgga aatgtaaaag
    17581 ccgtggacgg agtatccttc aatattcaga ggggacagat tcttggaata gtaggagaat
    17641 cgggctgcgg aaaatctact atgggaagaa ccatcttaag attgttgcca ccgacaggag
    17701 gtgaagtctt ttatgaaggt aaaaatgttt ttaatatgag taaaagagaa atgaacaaaa
    17761 tgcgaaccaa aatgcagatt atatttcaag acccatattc ttcattgaat ccaaggcttc
    17821 ctgtttatga tatagtgggc gaagcaatgc tacagcatgg tattgtaaaa aataaaaagg
    17881 aaatgaagga aaaggtaatt gaaataatcg gtaagtgcgg gctttccccg gaacaggcag
    17941 accgttaccc ccatcagttt tccggagggc aacgtcagcg tatttgcatt gcaagggcct
    18001 tagccgttaa tcctgatttt atggtctgcg acgaagcagt ttctgcattg gatgtttcta
    18061 tacagtcaca aataataaat ctgttaaaag atgcacagga agattacggg cttacctata
    18121 tgtttatatc tcatgacctg tcgatagtaa aatttatcag tgatgagata gcagttatgt
    18181 accttggtca aataattgag aagggcagta aaaaacaaat attttcaaat cccctccacc
    18241 cgtacacgga agcattattg tcagctgtcc cttcacttaa acaaaacaaa agaagaatag
    18301 ttttgaatgg ggatatacca tcgctggtaa atccgccaaa aggatgtcgt ttctatacac
    18361 gatgtcccta tgtaaaagat atatgcaagg aagaaatacc tgaatacagg gaaataaata
    18421 ccggacattt tgcaatgtgt cataaggtaa atggagtatt ttaatgtttt taaaaaagaa
    18481 tagagtagat gaagatttgg aaagaattcg ggaagctaac cttcccaaca aagaagaaat
    18541 aaaaatgaac gcgaagcatt cagatgacct tagtctggaa aaaggtgatc ttcttgcgat
    18601 ggtattggca gtaatatctc tgatactgcc ctatgtaatt gcttttttgg gggctatcgg
    18661 agttgtgata ttttttatgt atttgttttt tagttgaaaa atatacgaca ataagctata
    18721 gcagaggaat atggaagtat ataaaaaatc gggatttctc ccgatttttt cttttacagc
    18781 tgcttatatt ctttgaacgt tagccgcttg gtctcctctg tttccttgag taacatcaaa
    18841 gcttactctt tgaccttctt caagagtttt gaatccgtct ccctgaatag ctgaaaaatg
    18901 tacgaaaacg tcatttccat catcttttgt gataaatcca aatccttttt
//
LOCUS       DAOPUE010000032        18227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3114_length_18227_cov_9.770526, whole genome shotgun sequence.
ACCESSION   DAOPUE010000032 DAOPUE010000000
VERSION     DAOPUE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18227
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_18227_cov_9.770526"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKH42_04575"
     CDS             <1..339
                     /locus_tag="PKH42_04575"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOA19737.1"
                     /translation="MTEAQEAPKAEAESAAPGMDSSDTNVQVEGVQEGDIIKNDGKYI
                     YINTNRGLKIIDSNPDAPKIVYTVPVPENTSISEIFTAGNKLIVIGQNNLFYIMDNRQ
                     LTALICLSLP"
     gene            339..1664
                     /locus_tag="PKH42_04580"
     CDS             339..1664
                     /locus_tag="PKH42_04580"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOA19738.1"
                     /translation="MPPRYYQDRTDVLIYNIENIEKPVLEREYLFDGNYLSGRVIDDS
                     FYLINNKYLNLGYRYYDNKDIDIPLPYYTDVLNYEDYKFGYDEIKYFPNYIDTRYMYI
                     IGIDLNDTKSKPDVDVYLGGTDTIYVSKENLYAAVADYSYNYIVRPLEEFSPEYTTST
                     VIYKFELKDGKIGTAVQGEVPGTIINQFSMDEHNNTFRIATTTGDMWRNTSKNNVYIL
                     DEDLKVLGKLEGLAEGERIYSTRFAGDRMYMVTFRQVDPLFVIDTSNPRAPELKGMLK
                     IPGYSTYLHMVDENHILGFGYDTEVNQWGGTVNGGLKISMFDVSNVNKPKETFTEIIG
                     KSGTYSELLYNHKALMFSLNKGLMAFPVNRTAENYKSDFSGAYVYNVSNDSISLKNTI
                     THKESEFDSYGDNIKRIIYIGDYLYTFSENKMQIHGINTNKKVNELIIK"
     gene            1723..2313
                     /gene="yfbR"
                     /locus_tag="PKH42_04585"
     CDS             1723..2313
                     /gene="yfbR"
                     /locus_tag="PKH42_04585"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HOA19739.1"
                     /translation="MKKSNFFAFISRMKYIDRWGLMRNINKENVSEHSLDVAVIAHAL
                     AVIQKNRLNMDVNPEKTALYAIYHDVSEIFTGDMPTPVKYYNSVIKSAYKGVELSVNE
                     RLLNMLPEDFKDEFKDILIPKEEDKDIWKTVKAADKISAYIKCLEEEKSGNKEFLKAK
                     QSLLSTIKNMNMPEVKIFMDEFLEGYSLTLDEMEMS"
     gene            complement(2330..3067)
                     /locus_tag="PKH42_04590"
     CDS             complement(2330..3067)
                     /locus_tag="PKH42_04590"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOA19740.1"
                     /translation="MNILDYLIMGFIFGGIGTSIGGLISVLIFKPSEKIISGILSFAA
                     GIMLAVTSFDLMPQAYEIGGFFIVTLGLVIGLIIVFYANDLIPLNKLKQYKGKKLSYI
                     KMSLIIIISISLHNFPEGVAVGSSYVYSDNLGVKIGLLIAAHDIPEGISVGVPLIMAG
                     LSPVMVVFLTLLTGIPTALGAVFGALVGGVSDYFIAFCLSAASSSMLYVSANELIPEA
                     NLLYNGKSSSVLLLLGFMCGCYISFAV"
     gene            3272..4330
                     /locus_tag="PKH42_04595"
     CDS             3272..4330
                     /locus_tag="PKH42_04595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOA19741.1"
                     /translation="MENKINTKVVKVDPENIDCNIIKEAADIIKRGGLVVFPTETVYG
                     IGADALNDEAVDKIFKAKGRPQDNPLIVHIAEFHELYDLALEVPEKAELLAEKFWPGP
                     LTMILYKKKILSDKITAGLNTAAIRLPENKIALALIRESKKPIAAPSANTSGSPSPTE
                     ASHVIEDLMGKVDMIIDGGSTYIGLESTVVDMTSSVPMILRPGGVTAEDIISVLGECS
                     YDPAIIKSEEIVIPKSPGQKYRHYSPKAKVILYKGKIERIVEKINEDYDKFTSLGLKT
                     GIMSTAQTEEFYKGKINICIGDRTKLLTISSNLFKGLRDFDHMGVNVILAEAVDEKGL
                     GKAIMNRLGKSASETVEV"
     gene            4332..4820
                     /locus_tag="PKH42_04600"
     CDS             4332..4820
                     /locus_tag="PKH42_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOA19742.1"
                     /translation="MNILFVCTGNTCRSPMAEGIFKDMLMKNNINNISVSSAGISVFP
                     GEHASEKAIKALKEKGIDITGHRARQLLDEINNVDLILTMTLSHKMIISDYLKNETDE
                     KSPRLFTLKEFAAKLSGEKLTDTDIVDPYGKNYNFYKNSRDEIEKELIKILKNLDKLE
                     GI"
     gene            4817..5272
                     /gene="rpiB"
                     /locus_tag="PKH42_04605"
     CDS             4817..5272
                     /gene="rpiB"
                     /locus_tag="PKH42_04605"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOA19743.1"
                     /translation="MKIVLASDHGGFELKEAIKKHLIKKGYDINDIGVYDTKSVDYPD
                     YGKRAALMVADKEADRGIIVCGTGIGISIAANKIKGIRCALCTNEYMARMSRMHNNAN
                     MLALGGRVTGIGLATDMVDVWLSTEFEGGRHENRVNKIMDIEISKEEEK"
     gene            5272..6300
                     /locus_tag="PKH42_04610"
     CDS             5272..6300
                     /locus_tag="PKH42_04610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOA19744.1"
                     /translation="MTANLIAFISAVVISFVMTPWARRLAIKVGALDIPKEARKVHNK
                     PMPYFGGLAIYVSIIACMFVYMPHTVTNYYIMAGATVIVLAGVVDDMYGMPAKIKLLI
                     QIIAALIAIRGGVQIHFITNPLSETGMSYLFDWLSFVITLFWLVGITNTINLIDGLDG
                     LASGVASIAATTLLFTAARLGHDFIVMQCAIIAGASLGFLPFNFNPAKIFMGDTGSLL
                     LGYMLAVTSVSGMVKSVAVVALAVPVFALGLPIFDTTFAIIRRYINKKPIMEADKEHL
                     HHKLMGVGLNQRQTVLIMYFISMLLGIVSVIIADADPFTGLTVATAVVIIAFIFAKKA
                     GLFVRKEQ"
     gene            6377..7498
                     /gene="wecB"
                     /locus_tag="PKH42_04615"
     CDS             6377..7498
                     /gene="wecB"
                     /locus_tag="PKH42_04615"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009221699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOA19745.1"
                     /translation="MDKIKVLVVFGTRPEAVKMAPIVKALKKDEDNFISKICVTAQHR
                     DMLDQVLRIFDIEPDYDLNIFENGQTLTQITCRALTGLEEVIEDFKPEIILVQGDTTT
                     VFAGALAAFYHQVKIGHVEAGLRSGNLYSPYPEEANRMMTGVLTDFHFAPTETSKKNL
                     IREGYDENKIFITGNTSIDALRWVIDDNYKFEEEIINNIDFENKRVILLTAHRRENIG
                     KPMENIFSALKDIAEENQDVEVIYPMHLNPKVREIAHRIFGNLENVHLIEPLDYLPFT
                     NLMNKCYLVVTDSGGVQEEAPSLGKPVLVVRRETERPEGIEAGTAKLAGTDRENIYNE
                     LHLLLNNEEEYKKMANAVNPYGDGKAAEYIIQAIKSNYK"
     gene            7674..7895
                     /locus_tag="PKH42_04620"
     CDS             7674..7895
                     /locus_tag="PKH42_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOA19746.1"
                     /translation="MLEKIKEILVEELDVEEDSITLDSKIKEDLGADSLDLFELINRL
                     EDELDVVIEEDDYGKLITVGDIVNYLTDK"
     gene            7928..8899
                     /gene="fabK"
                     /locus_tag="PKH42_04625"
     CDS             7928..8899
                     /gene="fabK"
                     /locus_tag="PKH42_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOA19747.1"
                     /translation="MLRRVMIYTKICEMLNIKYPIFQGGMAQISDAGLAAAVSEAGGL
                     GIIASGNNPAEKIKAEIHRIRELTDKPFGVNVMLLSPYAKEVSELLIEEKVPAIITGA
                     GNPGKYMKIWKEAGIKVIPVVPSVAFAKHFEKAGVDAVICEGTEAGGHVGELTTMCIT
                     PQVVDAVNIPVISAGGIGDGRGIAAAFCLGAQGVQVGTRFVLAKECSVHQNYKDKIKK
                     AGDTDTAVTGRKTGHPVRVLKNRLVTKFKELEKTNATVEEMESLGRGKLYMAAVEGDV
                     DYGSVMSGQIAGMVNKEQSCKEIIDEMFEEAEKIMKNLVFIAGGGNE"
     gene            8892..9827
                     /gene="fabD"
                     /locus_tag="PKH42_04630"
     CDS             8892..9827
                     /gene="fabD"
                     /locus_tag="PKH42_04630"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOA19748.1"
                     /translation="MNKTAFVFPGQGAQYAGMGKDFYDNFKESREIFHRANEALGFDI
                     TKLCFEGPNEELSITKITQPALLTVCLAIYEVLKKRADDSVVMGGLSLGEYSALTAAG
                     AMDFETAVQLVYKRGNYMQNTVPLGEGGMLALLGCTDEEAIAFCKDITLKYGLLEPAN
                     FNYPGQIVAGGKSVAIDNAIDKAPRFNIKKAVKLQVSAPFHTSMLKPAGDKLKEDLAK
                     IIFKKPVTNVISNVDSVYYEKSKIAEKLEKQVYNPVRWEACVRKMIADGVNTFVEIGP
                     GKTLSGFIKRIDKNVKSINIENVQGLNTYLQFAGA"
     gene            9848..>10578
                     /gene="fabG"
                     /locus_tag="PKH42_04635"
     CDS             9848..>10578
                     /gene="fabG"
                     /locus_tag="PKH42_04635"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOA19749.1"
                     /translation="MKTAIITGASKGIGAVIAKRLNELNYNLVLNYRSNTALIEELID
                     NFTNKDTNNIIVKCDISLYEDAKKLIDEAYNNFGTVDVLINNAGITKDNILPLMTEEE
                     FDEVIDINLKGTFNCCKHIARRMIKQKYGRIINISSVVGLSGNAGQVNYAASKAGVIG
                     MTKSMARELGKKNILVNAVAPGFIKTEMTDKIPEDIKAVMLKNIPLQRLGQPEDIADC
                     VEFLISDKASYITGQVISVNGGFYM"
     assembly_gap    10579..10678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10692..11927
                     /gene="fabF"
                     /locus_tag="PKH42_04640"
     CDS             10692..11927
                     /gene="fabF"
                     /locus_tag="PKH42_04640"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOA19750.1"
                     /translation="MKRRVVITGMGVVSPIGSGDIFWENVKKGVCGIDFIESFDTSDF
                     SVKIAAEVKDFDPTLYMEKKEAKRMDRYSQFAIAAADIAVKDSGLNTKEVDSDRFGCI
                     VGSAVGGIHTIEEEHAKLLEKGPGKISTFFIPMMLSNMASGLVAVKYQAKAVNVSTVT
                     ACATGANAIGESYRRIQDGTCDVMIAGGAEAAITPCSIAGFTALTTLSTTRDINRASI
                     PFDKDRHGFVMGEGAGVMVLEDYEHAVKRGAKIYGEIVGYGATCDAYHMTSPDPSSEG
                     ASKSMLNAILDAGIEPKDITYINAHGTSTLYNDKFETQAIKNVFKEESENIYVSSTKS
                     MTGHLLGAAGAVEAIICVKAIEDSFIPATINHKETDPELGLNFVPNVGISKDFEYALS
                     NAFGFGGHNATLVVKKFKG"
     gene            11932..12357
                     /gene="fabZ"
                     /locus_tag="PKH42_04645"
     CDS             11932..12357
                     /gene="fabZ"
                     /locus_tag="PKH42_04645"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOA19751.1"
                     /translation="MVYNINDIQNVIPHRYPMLLVDKIIELQEGQKAVGIKNLTMNEQ
                     FFQGHFPGNPVMPGVLIIEALAQTGAFAILSLEKFKNKTVYFGGIQSAKFKRKVLPGD
                     TLKLVTEITKMKSTFGIGSAVAYVDDQVAAEATLIFAVE"
     gene            12678..13004
                     /locus_tag="PKH42_04650"
     CDS             12678..13004
                     /locus_tag="PKH42_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19752.1"
                     /translation="MSIEVIKVIKEAEEKAEAIKKDAAAQARLVITNANAQAQDILDE
                     ARKTAESHRSEVLSKAESEGQLLYDGIINDAAKECDKILTKANDNMDAAASIILERIV
                     KTSDNS"
     gene            13006..14973
                     /locus_tag="PKH42_04655"
     CDS             13006..14973
                     /locus_tag="PKH42_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOA19753.1"
                     /translation="MNKVNIIGLDSIKTELMKRIMDLGVVEISSQDTKLTDPEWTSYV
                     KKDGNENVVNNLDVKISQINEVLNTLELYDTSKRPLFTTRKTISSDEFRKKLEENNSV
                     SENVAKVLELNKSLSELCSEGNKIEASILSLKPWSSYDIPLDMTETKYSSIIMGTIPN
                     IVEIDELKSQLLQTTDRCIINVIGSDKDQYYCSFICLTEEKEDILEFLKQYGFSAVIF
                     KDLSGTAAENIIQYENSLKEISAKRETIDKRITELVSCKEEIQLFHDHLIIERDKNKI
                     LSNMLKTDTTFYIEGWIPEVSKDKVQKVLDEYECWYDFKKPEEGEEFPILLKNNAFSG
                     PFDSITELYSLPSPSNIDPTTVMAPFYVLFFGMMLGDVGYGFLFSLGCFIILKKFDIE
                     GTFGKMLKVFFWGGISTMFWGVMYGSWFGDGLVVGARHLFNINLPLTYVWVDPLKEPM
                     TVLLVSFIMGLVHLFVGMGMQAYMLIRDGKIKDAITDIGFWYLFIIGPILWLAGDMII
                     PGSPLPVIGKWMTLFAVIGLVLTQGRAKDNIISKLISGVLSLYGITGYLGDVLSYSRL
                     LALGLATGVISSVLSLIGSMVGGIPGKIMFVLVFVFGHTLNLAINGLGSFVHSARLQY
                     VEFFGKFYEGGGDAFNPFTKKTKYIKIKEEI"
     gene            14974..15450
                     /locus_tag="PKH42_04660"
     CDS             14974..15450
                     /locus_tag="PKH42_04660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOA19754.1"
                     /translation="MELTMGGFLAILGAAVASMAGIGSAWGVAIAGMAAGGVIAEDPK
                     KFGPTLMLQALPGTQGIYGLLIAFIIMNKIGVLGGNIAALTTPQGLMFLAAALPAGIT
                     GTVSAIWQGKVAATGVHLIGKRPEEIAKAMVYAAMVETYAVLALLASFLMVNGIQI"
     gene            15488..16069
                     /locus_tag="PKH42_04665"
     CDS             15488..16069
                     /locus_tag="PKH42_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOA19755.1"
                     /translation="MSIDNITSKILSDAQNNADSTLMNAENTKQEIITKAKSEAETII
                     KTEAERAEKDAVDLKNRRVSAAELQGRKMLLSAKQEAIKKSFDKAIEKLKAMPEDEYL
                     NFIAQEILKIPNCEGSIVLNAKDRENIGERLVKTVNEKLGAEKVVLSKNTVKASGGFV
                     LKRGSVEINSTFETLLDSMKDELTGEIANALFK"
     gene            16097..17107
                     /locus_tag="PKH42_04670"
     CDS             16097..17107
                     /locus_tag="PKH42_04670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HOA19756.1"
                     /translation="MAKIKESDYLYSASRVKSIEKHMLTRERAEKMIDAKTMEDAIRV
                     LDDCYYGYLNEITDFDNYESLLADEHKKTYDFIKSIAPDAEHFNIFLYPYDYHNLKVL
                     MKNEYLNKDETDLLVDTGTIDLNVLKYALKERNFSDLTENMGKALNEIIEDFPKTKDP
                     QLIDIILDRYCFDEMLKAAEKTENKFIIDYVKMQIDSINIKTYVRLKKMNKSWDFFSK
                     VFLHGGTIHEHIFIKNYDEPFDKFGELLLAYDFKDVFLEGTEALVETGKYTKLEKLLD
                     NKLMEHVKSAKYVPPYGIETLAGYLIAKDNEIKIARIILAGKTAGISPELIRERLRET
                     YV"
     gene            17100..17420
                     /locus_tag="PKH42_04675"
     CDS             17100..17420
                     /locus_tag="PKH42_04675"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOA19757.1"
                     /translation="MYKIGIIGDKQSVQGFKAVGLNVFDCNKAQEARRILHNIADDNY
                     AIIYVTESLYDEIKELIYEYNEERLPAIIPIPGMQGTLGIGIGNIKKAVEKAVGADIL
                     FSND"
     gene            17453..>18227
                     /locus_tag="PKH42_04680"
     CDS             17453..>18227
                     /locus_tag="PKH42_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOA19758.1"
                     /translation="MSQGRVVKISGPLVIADGMKEADMFDVVRVSEQGLIGEIIEMRG
                     EMASIQVYEETSGLAPGAPVVSTGAQLSVELGPGLIETIYDGIQRPLEEIRRIAGPNI
                     TRGISIPALNREKKWEFVPVVKAGDKVQAGDIIGTVQETIVVEHRIMVPYGISGEVVE
                     IKEGSFTVEETVCVVKKDNGEDVNLTMMQKWPVRKGRPYKEKLVPEAPLLTGQRVIDT
                     LFPISKGGVAAVPGPFGSGKTVVQHQLAKWADAEIVVYIG"
BASE COUNT         6461 a         2637 c         3916 g         5113 t
ORIGIN      
        1 atgactgaag ctcaagaagc accgaaggcc gaagcagaat ctgctgctcc cggcatggac
       61 agctcagata caaatgttca agttgaaggt gttcaggaag gggatattat taaaaatgac
      121 ggaaaataca tttatattaa tacaaacaga ggcttaaaaa taattgattc aaatcctgac
      181 gccccaaaaa tagtttatac agttcctgtc cccgaaaata caagtatcag tgaaattttt
      241 acggcaggaa ataagctgat tgtaatcggt cagaacaacc ttttctatat tatggataat
      301 cggcaattga cagcattgat atgcttgagc cttccataat gccgccgaga tactatcagg
      361 acagaaccga cgtattgatt tataatatag aaaatattga aaaaccggta cttgaaaggg
      421 aatatctttt tgacggcaac tatttatcag gcagggttat tgatgacagt ttttatttga
      481 taaataacaa gtatcttaac ttaggataca ggtactacga taataaggat attgatatac
      541 ctcttccata ttatacggat gtgttgaatt acgaggatta taaatttgga tatgatgaaa
      601 tcaagtattt tccaaattat attgacacaa gatatatgta tataatagga attgatttaa
      661 acgatacaaa atcaaaaccg gatgttgatg tgtacttagg cggcacggat acaatttatg
      721 tttcaaagga aaacttgtat gcggcagtag ccgattattc atataattat atagttcgtc
      781 cgcttgagga atttagccct gagtatacta ctagtacggt aatctataaa tttgaattga
      841 aagacggcaa aataggaact gctgtccaag gtgaagttcc gggaactata attaatcaat
      901 tttcaatgga tgagcataac aacaccttta gaattgcaac aactaccggt gatatgtgga
      961 ggaatacatc caaaaacaat gtatatattt tggatgaaga cctaaaagtt ttgggaaaat
     1021 tggaaggttt ggctgaaggt gaaagaattt acagcacacg cttcgccgga gacagaatgt
     1081 acatggttac attcaggcag gttgacccct tgtttgtaat tgacacatca aacccaagag
     1141 ctcccgagtt aaagggaatg cttaaaatac cgggatacag cacatatctt catatggttg
     1201 atgaaaatca cattttagga tttgggtatg atactgaagt aaatcaatgg ggcggaaccg
     1261 taaacggcgg cttaaaaatt tcaatgtttg atgtttcaaa tgtgaataaa ccaaaagaaa
     1321 catttacgga aataataggg aagtcgggaa catattcaga gctcttgtac aatcacaagg
     1381 cattgatgtt ttcattaaat aagggattaa tggcatttcc cgtaaacaga acagcagaaa
     1441 attataaaag cgactttagc ggagcatatg tatataatgt ttccaatgac agcataagtt
     1501 taaaaaatac aataactcac aaagaatctg aatttgattc ttacggagat aatatcaaaa
     1561 ggataattta tatcggtgat tatttgtaca cattttctga aaataaaatg caaattcacg
     1621 gcatcaatac aaataagaag gttaatgagt taataattaa ataaataaag gtgccaggca
     1681 cctttttggg tgcctggcac caatatacct atgggagagc ttatgaaaaa gtctaatttt
     1741 tttgcattta tatcaagaat gaagtatatt gaccgctggg gcttaatgag aaatataaac
     1801 aaggaaaatg tatctgagca cagtcttgac gttgctgtaa ttgctcatgc attggctgta
     1861 attcaaaaaa accggttgaa catggacgta aatccggaaa agactgccct ctatgcaatt
     1921 tatcacgatg tatcggaaat atttacggga gatatgccca cacctgttaa atattacaat
     1981 tcagttatta aatctgccta taaaggtgtg gagttgtcgg taaatgaaag gcttttaaat
     2041 atgcttccgg aagattttaa agatgagttt aaggatattt tgattcctaa ggaagaagac
     2101 aaagatatat ggaagaccgt aaaggctgcc gataaaataa gtgcatatat taagtgctta
     2161 gaagaagaaa agtcaggcaa caaggaattt ctaaaagcaa aacaatcatt gctttcgaca
     2221 ataaaaaata tgaatatgcc tgaagtgaaa atatttatgg atgaattctt ggaaggttac
     2281 agccttacct tggatgaaat ggagatgtcc taaccctatt tttaaataat cataccgcaa
     2341 aggatatata acatccgcac atgaaaccta gaagcagcaa tacagagctg ctttttccgt
     2401 tataaagtaa gtttgcttca ggaattaatt cattggctga cacataaagc atgctgcttg
     2461 atgcagcgga aagacaaaaa gctatgaagt aatccgatac acctcccacc aaagcaccga
     2521 atacggcccc cagagcagtt ggaatacctg taagaagagt caaaaaaact accattacag
     2581 gagataatcc tgccataatc aaaggaacac caacacttat accttccggt atatcgtgag
     2641 ctgcgatgag taatcctatt tttactccta aattatctga atacacatag cttgagccca
     2701 ccgcaacacc ttcggggaaa ttgtgcaagc ttatactgat tattataatt aagctcatct
     2761 tgatataact tagctttttt cctttatatt gctttaattt attcaaggga ataaggtcat
     2821 tagcataaaa aactattatt aggcctatta caaggcctaa ggtaactata aagaaccctc
     2881 cgatttcata tgcttgaggc atgagatcaa aggatgttac cgctaacata attcctgctg
     2941 caaagcttag aattccagat attatttttt cgcttggttt aaatataagt acggaaatca
     3001 atcctccgat tgaagttccg attccgccaa aaataaatcc cattatcaaa taatccaata
     3061 tattcacaat taatctcccc cttatgtccc tctagcatgg ggacatgagg tgtgtccccc
     3121 taccttatat atatgcgctt taactgattt aagaaattaa aaaattatta atcagtaaaa
     3181 ttcttgtaca taaagttatt gtagtatata atattacgag aacattcctt catgggatga
     3241 cggcaaatcg ccaagcaaga gaaggtatat gatggaaaat aaaataaata caaaggtagt
     3301 aaaggttgac cctgaaaata tagattgtaa tattattaaa gaagctgcag atattattaa
     3361 aaggggaggt cttgttgttt ttcctacgga aacagtctac ggaatcggtg ctgatgcatt
     3421 gaatgacgaa gcagtcgata aaatattcaa ggcaaaggga cgtcctcagg acaatccttt
     3481 aattgtacat attgcagaat ttcatgaatt gtatgattta gctttggagg tgccggagaa
     3541 ggcagagctt ttggcagaaa aattttggcc gggaccacta acgatgattt tatataaaaa
     3601 gaaaatatta tcagataaaa ttactgcagg attaaataca gctgccatac ggctgcctga
     3661 aaataaaatt gcacttgctt taataagaga aagcaaaaaa cctattgcag cgcccagcgc
     3721 aaatacatcg ggcagtccaa gtcctacgga agcttctcac gtaatagaag atttaatggg
     3781 aaaggtggac atgataattg atggaggaag tacttatatc ggtcttgaat caactgttgt
     3841 ggatatgaca agctccgtgc ccatgatatt aagaccgggc ggagtgacag cagaggatat
     3901 aattagtgtt cttggtgaat gcagttatga ccctgcaatt ataaaaagcg aagaaatagt
     3961 tattccaaaa tcaccgggtc agaaatacag gcattattct cccaaggcaa aagttatttt
     4021 gtataagggt aaaatagaaa gaattgtaga aaaaatcaat gaagattatg ataaattcac
     4081 gtctttggga ttaaagacgg ggattatgtc aactgctcaa acagaagagt tttataaggg
     4141 gaaaataaat atttgcattg gagacagaac aaaactgctt acaatttcgt caaacttatt
     4201 taagggttta agggattttg accacatggg agtaaatgta atacttgcgg aagcagttga
     4261 cgaaaaaggt ctcgggaagg ccataatgaa caggctcggt aagtcagcca gcgaaacagt
     4321 cgaggtatag aatgaatatt ttgtttgttt gtacgggcaa tacatgcaga agccctatgg
     4381 cagaaggtat ttttaaggat atgcttatga agaataatat taataatatt agcgtatctt
     4441 ctgccgggat aagtgttttt cccggagagc atgccagtga aaaggcaata aaggctttaa
     4501 aagaaaaggg aattgatata acagggcaca gagcaagaca gctgcttgat gagattaata
     4561 atgttgacct gatattaaca atgaccttat ctcataaaat gattatttct gattatttaa
     4621 aaaacgagac agatgaaaag tctcctcgtc tttttacctt gaaagaattt gctgcaaaat
     4681 taagcggtga aaaattgaca gatactgata ttgtcgaccc ttatggcaaa aattataatt
     4741 tttacaaaaa tagcagagat gaaatcgaaa aagaactaat aaagatactc aaaaatttag
     4801 ataagctgga gggaatatga aaatagtatt ggcaagcgac catggaggat ttgaattaaa
     4861 ggaagcaatt aagaaacatt taataaaaaa gggctatgat ataaatgata taggtgttta
     4921 tgatacaaaa tccgttgatt accctgatta cggtaagagg gctgccttga tggtagctga
     4981 taaagaagcc gatagaggta ttattgtttg cggaaccgga ataggtattt ctattgcagc
     5041 aaacaaaatt aaaggaataa gatgtgcctt gtgcacaaat gaatatatgg ccagaatgtc
     5101 aagaatgcac aacaatgcaa acatgcttgc attaggcggc agagttaccg gtataggtct
     5161 tgcaacggat atggttgatg tttggctttc tactgaattt gaaggcggaa gacatgaaaa
     5221 cagggtaaac aaaattatgg atatagaaat ttctaaagaa gaggaaaaat aatgactgca
     5281 aatttaatag ctttcatatc agctgttgtt atatcattcg taatgactcc atgggcaaga
     5341 agactagcaa taaaggtggg agcattggat atacccaagg aagcaagaaa agtccacaac
     5401 aagccgatgc cttactttgg aggacttgca atatatgtat caataattgc atgtatgttt
     5461 gtttatatgc ctcacactgt caccaactat tatattatgg caggggcgac agtaattgtg
     5521 cttgcaggtg ttgttgatga tatgtacggc atgcctgcaa aaattaaact cttgatacag
     5581 ataatagcag cattaatagc aataagaggc ggcgtacaga tacattttat aacaaatccg
     5641 ctttcggaaa caggtatgag ctacttgttc gattggttat cttttgttat tacattattc
     5701 tggttagtgg gtattacaaa tacaataaat ttaattgacg ggcttgacgg gcttgcttca
     5761 ggggttgcca gcattgcagc cacaactctt ttatttaccg cagcaagatt gggacatgac
     5821 ttcattgtga tgcaatgtgc aattattgca ggtgcttcac tggggttttt gccttttaat
     5881 tttaatccgg caaaaatttt catgggagat acaggctcct tgttgctggg gtatatgctt
     5941 gcggttacat ctgtttcggg tatggtaaag agcgttgcag ttgttgcatt ggctgtcccg
     6001 gtatttgcat taggtctgcc tatatttgat actacgtttg caattataag aagatatata
     6061 aataaaaaac ctataatgga agcagacaag gaacatcttc atcataagct tatgggagtc
     6121 ggccttaatc agcggcagac agtcttaata atgtatttta taagtatgct tttgggaata
     6181 gtttcggtaa taatagcaga tgcagacccc ttcaccggtt tgacggttgc tactgcggtt
     6241 gttataattg catttatatt tgcaaaaaaa gcgggactgt ttgtaaggaa agagcaataa
     6301 gtgtcatcct gagcgtagcg aaggatctca tgagattcat cggactgata tattgttgcg
     6361 gttaatagga ggaaacatgg ataaaattaa ggttttggta gtgttcggaa cccgtccgga
     6421 ggctgtaaaa atggctccaa ttgtgaaggc tcttaaaaag gatgaggata atttcatatc
     6481 taaaatttgc gtaactgccc agcacaggga tatgcttgac caagtactga ggatttttga
     6541 tattgagccc gattatgatt taaatatttt tgaaaacggt caaacactta cacaaattac
     6601 atgcagagca ttaacagggt tagaagaagt aatagaagat tttaaacctg aaataatact
     6661 ggtacaggga gatacaacaa cggtatttgc aggagctctt gctgcttttt atcatcaggt
     6721 taaaataggt catgtggaag caggacttag aagcggcaac ctgtattcgc cttatcctga
     6781 ggaagcaaat agaatgatga caggtgtatt gaccgatttt cattttgctc cgacagaaac
     6841 atctaagaaa aatctcataa gagaaggcta cgacgaaaat aaaatattta taaccggaaa
     6901 tacttcaata gatgctttaa gatgggttat agatgataat tataaatttg aggaagaaat
     6961 aatcaataat atagattttg aaaataaaag ggtaatactc ttgactgctc acagaagaga
     7021 aaatatcggc aaaccgatgg aaaatatatt ttctgctctg aaggatattg cagaagaaaa
     7081 tcaggatgta gaggttatat atccaatgca cttaaatcct aaggtaagag aaattgccca
     7141 tagaatattt ggcaatttgg aaaatgttca tttgattgaa cccttagatt atcttccttt
     7201 cacaaattta atgaataaat gttaccttgt tgtcacagat tcgggagggg ttcaggaaga
     7261 agctccatca ttgggaaaac ctgtcttggt tgtgagaaga gaaacggaaa gaccggaagg
     7321 tattgaagca ggaactgcaa aattagcagg tacagacaga gaaaatattt acaatgaact
     7381 gcatttattg ctcaataacg aagaagaata taaaaagatg gctaatgccg taaatccata
     7441 cggagatgga aaggctgcag aatatataat tcaagcgata aagagcaatt ataaatagtg
     7501 acaacatccc cgtttttttg acactcaaaa aaacagggcc atgatgtagg caaaaaatat
     7561 attgacaagg gtgttttgtt gtattatact ttgaatatca aaatacttga atatgaataa
     7621 atctaataat caaagtaata gataatatta gtatattagg aggaaatatt atgatcttag
     7681 aaaagattaa ggagattttg gttgaagaat tagatgtgga ggaagatagt attaccttag
     7741 acagtaaaat caaggaggac ttaggagcag actcattgga cctatttgaa ctgataaaca
     7801 gacttgagga tgagctggat gtagtaattg aagaagacga ttatggtaag ctaataacag
     7861 tgggtgatat agtaaattac cttacagata aataataata aataaaagag acgccgcaag
     7921 gcgttttgtg ctaaggagag tcatgattta tactaagata tgtgaaatgc taaatataaa
     7981 atatcccatt ttccaaggag gaatggcgca aatatcggat gcaggattag cagcagccgt
     8041 atccgaagca ggagggcttg gcataatagc gtccggtaat aatccggctg aaaaaataaa
     8101 agctgaaata catagaatca gagagttgac ggataaaccg tttggagtaa acgtaatgct
     8161 tttaagccct tatgccaaag aggtcagcga attattgata gaagaaaaag ttcctgcaat
     8221 tataacgggg gcgggcaatc ccggaaagta tatgaaaata tggaaggaag cagggattaa
     8281 ggtaatacct gttgttccgt ctgtagcttt tgctaagcat tttgaaaaag caggggttga
     8341 tgccgtaatt tgtgaaggaa ctgaagcagg aggacatgtg ggagaattaa ccaccatgtg
     8401 tattactcct caggttgtgg atgctgttaa tattcccgta atatctgcgg gaggaatagg
     8461 tgacggaaga ggaattgcgg cagcattctg ccttggtgca caaggtgtac aggttggaac
     8521 aagatttgtt ttagcaaagg aatgcagcgt tcaccaaaat tacaaagata aaataaagaa
     8581 agcgggcgat acggatactg ccgtaacagg aagaaagaca ggtcatcctg tgagagtatt
     8641 aaaaaacaga ttagtgacta aatttaaaga attggaaaaa accaatgcaa ctgttgaaga
     8701 aatggagtca ttgggaaggg gcaaacttta tatggctgct gttgaaggcg atgtagatta
     8761 cggttccgta atgtcaggtc aaattgcagg catggtaaat aaagagcaaa gttgcaagga
     8821 aattatcgat gaaatgtttg aagaagcaga aaaaatcatg aagaatttag tatttatcgc
     8881 gggaggcggc aatgaataaa acagcttttg ttttcccggg ccagggtgct caatatgccg
     8941 gcatgggaaa agatttttat gataatttta aagaaagcag agaaatattt catagagcaa
     9001 atgaagcatt aggattcgat ataactaagc tttgctttga aggacctaat gaagaactca
     9061 gcattacaaa aataactcag ccggcacttt tgacagtatg tcttgcgatt tatgaagtat
     9121 taaagaagag ggctgatgat tctgttgtaa tgggcggatt aagccttggt gaatattctg
     9181 ccttaactgc tgcaggtgca atggactttg aaacagccgt acagttagta tataaaagag
     9241 gaaattatat gcaaaatact gttcccttag gagaaggagg catgttagct cttttaggct
     9301 gtacggatga ggaagcaatt gctttttgca aggatataac tttaaaatat ggtttattgg
     9361 agcctgctaa ttttaactat ccgggacaaa tagtagcagg tggaaaaagt gttgccattg
     9421 ataatgctat tgataaagca cctcggttta atatcaaaaa agctgtaaag cttcaagtga
     9481 gcgctccatt tcacacatca atgcttaaac ccgcaggtga taaattaaaa gaagatttgg
     9541 ctaaaataat attcaaaaaa cctgtcacta atgttatatc aaatgtggat agcgtatatt
     9601 acgaaaaaag taaaattgca gaaaagcttg aaaaacaagt gtataatcct gtcaggtggg
     9661 aggcgtgcgt cagaaaaatg attgcagacg gagtaaatac atttgtagaa ataggacccg
     9721 gtaagacact ttccggattt attaaaagaa ttgacaaaaa tgttaaatca ataaacattg
     9781 aaaatgttca aggattaaat acatatctgc aatttgcagg agcttaaaat acaaaggaag
     9841 gaaaaccatg aagactgcca taataacagg agcttccaaa gggataggag ccgtaattgc
     9901 caaaagattg aatgagttga attacaattt agtgttgaac tacagaagca acactgcttt
     9961 aatagaagaa cttatagata attttactaa taaagatact aataatataa tagttaagtg
    10021 cgatatatct ctttatgaag atgcaaaaaa acttatagat gaagcttaca ataattttgg
    10081 cacggttgat gtgcttatta ataatgccgg aattacaaag gataatatac ttcccttaat
    10141 gacagaagaa gaatttgatg aagttatcga tataaattta aagggtacct ttaactgttg
    10201 caaacatatt gcaagaagaa tgataaagca aaagtacggc agaattataa acatatcatc
    10261 ggtagttggt ctttcgggca atgcaggaca ggtaaactat gcagcttcaa aagcaggagt
    10321 aataggtatg acaaagtcca tggcaagaga attaggcaag aaaaatattt tggtaaatgc
    10381 tgtggcaccg ggatttatta aaactgaaat gacagataaa atacctgagg atattaaggc
    10441 agtgatgttg aaaaatattc ctcttcaaag attagggcaa ccggaagata tagcggattg
    10501 tgttgaattt ttaatatcag acaaagcgtc ctatattaca gggcaggtta tatctgttaa
    10561 cggcggattt tatatgtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
    10681 aggagagaat aatgaaaaga agagttgtta taacaggaat gggggtagta agccctattg
    10741 gctcgggaga tattttttgg gaaaatgtaa aaaagggagt atgcggaatt gattttatag
    10801 aatcttttga tacatcggat ttcagcgtaa aaatagcagc tgaagtgaag gattttgacc
    10861 ctactttgta tatggaaaaa aaggaagcca aaaggatgga cagatacagc cagtttgcca
    10921 tagcagctgc agatattgca gtcaaggatt cgggtcttaa tactaaggag gttgattccg
    10981 acaggtttgg ctgtattgtc ggaagtgcag tcggtggaat acatacaata gaagaagaac
    11041 atgcaaagct gttggaaaag ggaccgggaa agatttcaac ttttttcatt ccaatgatgc
    11101 tttcaaatat ggcatccgga ttagttgccg ttaagtatca ggcgaaggct gtcaatgtaa
    11161 gtacggttac ggcttgtgcc accggagcta acgcaatagg agaatcatac agaagaatac
    11221 aggatggaac ctgcgatgtt atgattgcag gaggcgctga agcagcaata actccatgct
    11281 ctattgcagg atttacggct cttactacat tatcaacaac aagagacata aacagagctt
    11341 ctattccatt cgataaggat agacatggat ttgtaatggg agaaggtgcc ggtgttatgg
    11401 tgcttgaaga ttatgagcat gctgtcaaaa ggggagcaaa aatatatggt gaaatagtgg
    11461 ggtatggtgc aacctgtgat gcatatcaca tgacaagccc tgatccaagc tcagaaggcg
    11521 catctaagtc aatgttaaat gctattttgg atgcaggtat cgagccaaag gatataacat
    11581 atataaatgc ccacggaaca agtaccttat acaatgataa atttgaaacc caggctatta
    11641 agaatgtttt taaggaagaa tcagaaaata tatatgtaag ctctacaaaa tcaatgacag
    11701 gacatttgct tggagcagcg ggggcagttg aagcaatcat ctgcgttaaa gctatagaag
    11761 attcctttat tcctgcaacc ataaatcata aagagacaga tccggaatta ggtttaaatt
    11821 ttgtgcctaa tgtgggaatt tcaaaggact ttgagtatgc cttgtcaaat gcatttggtt
    11881 ttggcggtca taatgcaacc ttagtagtaa aaaaatttaa aggatgaaat tatggtttat
    11941 aatataaatg atatacaaaa tgtaattcct cacagatatc ccatgctttt agtcgataaa
    12001 ataatcgagc ttcaagaagg tcaaaaggct gtgggaatta aaaatttaac aatgaatgag
    12061 cagttttttc aaggtcattt tcccggaaac ccggtaatgc ccggcgtttt aattattgaa
    12121 gctttagctc agacaggagc ttttgcaata ttgtccttag aaaaattcaa aaataagacg
    12181 gtttatttcg ggggaattca aagcgcaaag tttaaaagga aggttcttcc gggagataca
    12241 ttaaagcttg ttacagaaat aacaaagatg aaatcaactt ttggaatcgg atctgcagtt
    12301 gcttatgttg atgaccaagt tgcggcagaa gcaacgctca tatttgcggt tgaatagccc
    12361 tggacctttc tttagtaaag ggtatgcctc tgtttgcaaa tgaatataag aataactttt
    12421 atcactcttg tgatataagt tatttttttt gacatttacc gataaatttt actattgtaa
    12481 acaaatactt attaagtaaa gaagaatcat aatttatggt ttaatcaaag ttactttaaa
    12541 cactaaaatc caaaaatact cttcttttag tatttttgga tttagacaaa aacttgacaa
    12601 atgcttgttt ttactttaat ataagttaat gtggaatgat agaaaatttc tagaaatatc
    12661 ataaaggtgg tggtatagtg tcaattgaag taattaaggt aataaaggag gcggaggaaa
    12721 aggctgaagc aattaaaaaa gatgccgcag cacaagcaag attagttatt accaatgcta
    12781 atgcacaagc tcaagatatc ttggatgaag caagaaaaac agcagagtct cacagatctg
    12841 aagttttaag caaagcagaa tccgagggac agcttcttta tgacggcatt attaatgatg
    12901 ctgcaaagga atgtgacaaa attttaacaa aggcaaatga taatatggac gccgcagctt
    12961 caattatact ggaaaggatc gtgaagacca gtgacaatag ttaatatgaa taaagtcaac
    13021 ataataggtt tagacagcat aaaaacagag cttatgaaga gaattatgga tttaggcgtt
    13081 gtggaaataa gctcacaaga tacaaaactt acagaccccg aatggacttc ttatgtaaaa
    13141 aaagatggga atgaaaatgt agttaacaat ctcgatgtta aaatttctca gattaacgaa
    13201 gtactgaaca cccttgaatt gtatgacaca agtaaaagac ctttatttac cacaagaaaa
    13261 acgatatcat cggatgaatt tagaaaaaaa ttagaggaaa acaacagcgt atctgaaaat
    13321 gtagccaagg ttcttgaatt aaataagtca ttaagtgagc tatgctctga gggaaataaa
    13381 atagaagcat ccattttgag tttaaaacca tggagcagtt atgatattcc tctagatatg
    13441 acggagacta aatattcaag cattataatg ggtacaatac ctaatatcgt tgaaatagat
    13501 gagttaaaat cccagcttct tcaaacaact gacagatgta ttataaatgt aataggcagc
    13561 gacaaggacc agtattattg ctcatttatt tgccttaccg aggaaaagga ggatattctg
    13621 gaatttctaa aacaatatgg ttttagtgct gtgatattca aggatttaag cggtactgca
    13681 gcggaaaata ttatccaata tgagaacagc ttaaaagaaa tatctgctaa gagagaaaca
    13741 atcgataaaa gaataacgga attagtttcc tgcaaagaag aaatccaact tttccatgac
    13801 catctgataa tcgaaagaga taaaaataaa atattaagta atatgctaaa gacagataca
    13861 accttttata ttgaaggttg gatacctgaa gtaagcaagg acaaagttca aaaagtttta
    13921 gacgaatatg agtgctggta tgattttaaa aagccggagg aaggggaaga attcccgata
    13981 ttattgaaga ataatgcttt ttcaggacca tttgattcaa taacagagct gtatagtctg
    14041 ccttcaccat cgaatataga cccgactaca gttatggcac cgttttacgt tttattcttt
    14101 ggcatgatgc ttggggacgt aggctatggt tttctttttt cattgggatg tttcattata
    14161 ctcaaaaaat ttgatataga aggtactttt ggaaaaatgc ttaaggtatt tttttggggc
    14221 ggaatatcca ctatgttttg gggtgtaatg tatggaagct ggtttggaga tgggttagtt
    14281 gtaggagcaa gacatctttt caatattaac ttgcctttga catatgtgtg ggtagacccc
    14341 ttaaaagagc ctatgactgt cttattggta tcattcatca tgggtctcgt tcacttattt
    14401 gtaggaatgg gaatgcaggc ttatatgtta ataagagatg gtaaaattaa ggatgcaata
    14461 accgatatag gtttttggta tttatttata ataggaccta ttttgtggtt agcaggagat
    14521 atgataattc caggttcgcc attacctgtt ataggtaaat ggatgacttt atttgctgta
    14581 ataggtcttg tcctgactca aggaagagca aaagataata taatcagcaa gttaataagc
    14641 ggtgtattgt cgctatatgg aataacaggt tacttaggtg atgttctttc atattcgagg
    14701 cttcttgcac tcggacttgc aacaggtgtt atttcatctg ttctaagttt aataggctcc
    14761 atggtcggcg gcatacccgg gaaaataatg tttgtattgg tttttgtatt cggccataca
    14821 cttaatcttg ccataaacgg tcttggttca ttcgttcact cggcaaggct tcaatatgta
    14881 gaatttttcg gtaaatttta tgaaggtgga ggagatgcat ttaatccttt cactaaaaaa
    14941 actaaatata ttaaaattaa ggaggaaatt taaatggaat taacaatggg tggtttttta
    15001 gctattttag gtgccgctgt agcatctatg gcaggaatag gtagtgcatg gggagttgct
    15061 atagcaggaa tggctgctgg tggtgttatt gcggaggatc ctaagaaatt cggtcccaca
    15121 ttaatgcttc aggctctgcc cggaacacaa ggtatatatg gacttcttat tgcatttatt
    15181 ataatgaata aaatcggtgt acttggaggc aacatagctg ctttgacaac acctcagggt
    15241 ttaatgtttt tggcagcagc acttcctgcc ggtataaccg gaacagtatc agctatttgg
    15301 caaggtaagg ttgcagctac aggtgttcac ttgattggaa aaagacctga agaaatcgca
    15361 aaagcaatgg tatacgcagc tatggttgaa acatatgcag ttttagcact tcttgcatca
    15421 ttccttatgg taaatggaat tcaaatataa aggagtgtgt aaaaatttat tataaggatg
    15481 tgatgatatg agcattgata atataacatc aaagatttta agcgatgcac aaaataacgc
    15541 tgattctact ttgatgaatg ctgaaaatac aaagcaggaa ataataacca aagctaaaag
    15601 tgaagctgaa acaataataa agactgaagc ggaaagagca gaaaaagacg cagttgattt
    15661 aaaaaacaga agggtatctg ctgctgaact tcaaggcaga aaaatgctat tatcagcaaa
    15721 acaggaagct attaaaaaaa gttttgataa agcaattgag aagcttaaag caatgccgga
    15781 agatgaatat ttgaatttta tagcacagga gattttaaaa attcctaatt gtgaaggaag
    15841 tatagtttta aatgccaaag acagggaaaa tatcggtgaa agattagtaa agactgtcaa
    15901 cgaaaagctg ggagcagaaa aagttgtatt aagcaaaaat acggttaagg caagcggcgg
    15961 attcgtgctt aaaaggggca gcgttgaaat taacagtact tttgaaaccc ttttagattc
    16021 catgaaggac gaacttacag gtgaaatagc aaatgcgctt tttaaataag actcctgtcg
    16081 gaaaggagga atgactttgg cgaagattaa ggaatctgat tatttatatt ctgcttcaag
    16141 agtgaaaagt atagaaaagc atatgcttac ccgtgaaaga gcagaaaaaa tgatagatgc
    16201 aaaaacaatg gaagatgcca ttagagtatt ggatgattgt tattatggtt acttaaatga
    16261 aataacggat tttgataatt acgaaagcct ccttgcggat gagcataaga aaacatatga
    16321 ctttattaag tccattgctc ctgatgctga acatttcaat atatttttgt acccatacga
    16381 ctatcataat ttgaaagtat taatgaaaaa cgagtatttg aacaaggatg aaacagatct
    16441 tcttgttgat actggaacca ttgacttaaa cgttttaaaa tatgctttaa aagaaagaaa
    16501 tttttctgat ttaaccgaaa atatgggaaa agcattaaat gaaataattg aagattttcc
    16561 aaagacaaaa gaccctcagc tcatagatat tattctagac aggtattgtt ttgatgaaat
    16621 gcttaaagcc gcagaaaaaa cagaaaataa atttattatt gattatgtaa agatgcagat
    16681 tgattcaatt aatattaaga catatgtaag gcttaagaaa atgaataaat catgggattt
    16741 tttcagcaaa gtatttttgc atggcggtac catacatgaa catatattta ttaaaaacta
    16801 tgatgagcct tttgataaat ttggagaact tcttttagct tatgatttta aggatgtatt
    16861 tctcgaggga actgaagcat tagttgaaac aggaaaatat acaaagcttg agaaactgtt
    16921 agataacaag cttatggagc atgttaaatc tgccaagtat gttccgcctt acggaataga
    16981 aactcttgca ggatatctca ttgctaagga taatgaaatt aaaattgcac gaataatttt
    17041 agcaggaaaa acagcaggca tatcgccgga acttataaga gagaggttga gagagactta
    17101 tgtataagat aggaattata ggagataagc aaagtgttca agggttcaaa gctgttggct
    17161 tgaatgtatt cgactgcaat aaagcacaag aagcaagacg tatactgcat aatattgcag
    17221 atgataatta tgcaataata tatgttacgg aaagcttata tgacgaaata aaagaattaa
    17281 tatatgagta caatgaagag cgtcttccgg caatcattcc tataccgggc atgcagggaa
    17341 cactaggcat agggattggc aacataaaga aggcagttga aaaagcagtt ggtgccgaca
    17401 tattgtttag taacgattaa cgtaaaaaaa atgaaagcag gtgattagtt taatgagcca
    17461 aggtagagta gttaaaatat ccggtccgct ggttattgca gatggcatga aagaagccga
    17521 tatgtttgac gttgttcgtg taagcgagca aggccttata ggggaaataa ttgagatgcg
    17581 cggtgaaatg gcatccatac aggtttatga agaaacatca ggcttagctc cgggggcacc
    17641 ggttgtttca acaggagcac agctttctgt tgaattggga cccggactaa ttgaaacaat
    17701 atatgacgga atacaaaggc ctttggaaga aataagaaga atagcaggac ctaatattac
    17761 gagaggaata agtattcctg ctcttaacag ggagaagaag tgggaatttg tccctgtagt
    17821 aaaagcagga gacaaggtac aggcaggaga tataataggt actgttcagg aaacaatagt
    17881 tgtagagcat agaattatgg ttccttacgg aatttcaggg gaagttgttg aaataaagga
    17941 aggaagcttt actgttgaag aaacagtatg tgtagtaaaa aaagataacg gtgaagatgt
    18001 taacctaacc atgatgcaaa aatggcctgt aagaaaagga cgtccatata aggaaaaatt
    18061 agttcctgaa gcacctttgc taacaggtca gagggttata gatactttat tcccaatttc
    18121 aaagggtggt gttgcagctg ttccgggacc tttcggaagc ggaaagacgg tagtgcagca
    18181 ccagctggca aaatgggcgg atgctgaaat agttgtatat ataggct
//
LOCUS       DAOPUE010000033        18088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3148_length_18088_cov_10.771364, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000033 DAOPUE010000000
VERSION     DAOPUE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 18088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18088
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3148_length_18088_cov_10.771364"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..624
                     /locus_tag="PKH42_04685"
     CDS             28..624
                     /locus_tag="PKH42_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOA19759.1"
                     /translation="MKDPYEILGLKRGATKEEVKGAYRKLAKKYHPDMNVNNPLQDLA
                     EEKFKEIQWAYDEIMSGNADGPGYGSTYTGNNSKSTGDLFSARQYVNAGRFYEALSIL
                     RKTKVRNAEWNFLMGVCYVSLGSINQGMQHIQTAVNMEPSNMEYQNYLNQIYNMQRTY
                     QQRAYSYGGGSSSVDCCAQLICADCLCECLGGDLISCC"
     gene            615..1115
                     /locus_tag="PKH42_04690"
     CDS             615..1115
                     /locus_tag="PKH42_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19760.1"
                     /translation="MLLKSNNVAYLGVLLGLNQLFIILSSVIETNTIILMAAAALIVG
                     VVVVEFGGKAGIIFYIASCISGFFLTFNKIEIITYIYFFGIYSIIKHYIETKIFNKYI
                     SYAVKIASFNISILLMYFTAKLFINLPIRWWMIIAAQVLFIIYDYAYTIFINQYINFI
                     KPKLGR"
     gene            1339..3117
                     /locus_tag="PKH42_04695"
     CDS             1339..3117
                     /locus_tag="PKH42_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOA19761.1"
                     /translation="MLKKIKYAILLLLLTVLLTSCTESADADANTPPSDETNQEIPEI
                     IESELSEEEKAKLELEKRIAEREELEEQRKEELGEFYVPLIPIGEEREKKSVEVKALY
                     VTGHTAGNDLNKENIDAYGEYVKALAENNTQKARELFDKAEKANKFERIVGIALATEI
                     NGLVINVKDDNGFITYKSNIEIVKEMNKETPIPIKDIDNMLKILKEYDLYIIGRIVAF
                     KDKNFAVKSPEHAIQLKSGGIWLDPNSGKIPWINPFDKYVWDYNVAIAKEASLLGFDE
                     IQFDYVRFPDGAKTYNAIAEFPGRDGRDKDEAIADFLEYSKKELEPYNVNLGADVFGL
                     VTRTWDDYPEDIGQTWILIGKHVDNICPMVYPSHYSTGWYNLENPNAHPYLVVKGAME
                     EAIEKNSSMETPPIIRPWIQDFDWDGIAYGPDKVRDQIIAAKELGVYEYMIWNPSNVY
                     DPYAFMVTEKEKITAYPLNKGELDYREKSPADAAVKYLTGMLQDRHSYTYLMTPVSDR
                     VNDFDEFVKLREEDNIKLLSYKVYGYEMDANDSNKANVSLSYKIEVKNGDTTEIIEKD
                     NAKWQAIRELNIWKVKVNWDIGKNVH"
     gene            3244..4257
                     /locus_tag="PKH42_04700"
     CDS             3244..4257
                     /locus_tag="PKH42_04700"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOA19762.1"
                     /translation="MKKYNVAILGATGAVGQEMLKVLGERNFPINDLKLLASKRSAGA
                     IVEFNGEEYAIEEATESSFENIDVVLCAAENDISEKLSPAAVKAGAVVVDNSSAFRLY
                     DDVPLVVPEVNGEDVKNHQGIIANPNCSTTIALTAINELNKYSPIKRMIVTTFQAVSG
                     AGVNGIKELNQQIKDISEGKDVEINTFQHQIAYNVIPQIGSFNEMGYSQEEMKLLNEG
                     RKILHNPDLKVNCTCVRVPVYRSHSESITIETEKEITVEKARELLSKAAGVKLIDDLE
                     NNKYPMPLDSSDQDLIYVGRIRKDISGENSLVLWCCGDQIRKGAATNAVQIAEVLVKE
                     NLI"
     gene            complement(4320..6062)
                     /locus_tag="PKH42_04705"
     CDS             complement(4320..6062)
                     /locus_tag="PKH42_04705"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOA19763.1"
                     /translation="MKKAISLVLVIIIFFSAAQPCVLAADIETYNQLDKMVQRIVDEG
                     KSKGAVLSVVKDGNIELCKGYGLADDNLGIASDGEKIAFRIGSVSKTFVAVAAQILVE
                     KELLNMDSDISIYLDLDLKLTYPVSMRQLLTHTAGFEDMVTGMAFYNVSDTEPLEISV
                     RKYLPRQIYKPGEVISYSNYGIALAAYVIECITEQDFALFCEEKIFEPLNMNRTTFRF
                     MHDKAYVSKAYSPNGNETIEPYMNLYPEGSAVSTAEDMAKYMIWLMDGDDNRVLSPDS
                     KAELFKKQFTMSEEFEGIGLIWNRKEQDNKVYYDKKGETLNFYTRIALYSEADTGVFL
                     SFNTYVPEHEINNIMNKATDLIYGEKQEYTIKNADSSIKIGGLYVNNWSSFNTPEKIL
                     RYFIPNKMIYIDTVKDGHYINGQKMTLIDEDLYSSDLGLLKFKEVQNKIIMSSESAIT
                     YVKVPLWQHRIIQMIIPIIFIIVTFIIFIRELIKKFSNKNNKYSFAFALNSFFQIVSF
                     VILCLLMYKGIVTFSILKYAPYMKFLGWFIAALVISNIIIAIKNKKYKELLPAIWVLS
                     GILFSAWMLFFHIL"
     gene            6220..6927
                     /locus_tag="PKH42_04710"
     CDS             6220..6927
                     /locus_tag="PKH42_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA19764.1"
                     /translation="MNILVAEDEIDIRNLIKITLEDEGYSVITACDGIEALKKLKETP
                     VDLAILDIMMPRLDGFNLLRKIRENSTIPVIFLTARTDDLDKVLGLELGADDYIAKPF
                     SMSELIARVRAQLRRNNEYMLPKQENKTLLQYGDLTIDKESCCAYKNNEQIELNAKEY
                     KLLLHLMENPERVFTKKQLYNAVWDDDYYYDDNTIMVHISHLRNKIETDSQNPEYIKT
                     IRGLGYKLHKTGGYNEK"
     gene            6917..8323
                     /locus_tag="PKH42_04715"
     CDS             6917..8323
                     /locus_tag="PKH42_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA19765.1"
                     /translation="MKNKISHQFLLNYLTFFLMSLLAALFAYLLLSFADDVISKTLVK
                     NRYPSELIMKDNYADIDAESVVEHGGGVQVINENYEVVYSKGLDTLGKKQFSVSEFTD
                     FLVKSKEKGFKYHYDILYNPQAKFWLIVTFPTSMRLDISFVYNKEAASEDLKNVSFVF
                     ISVFILYLLLLGLLAFIFSKITSIHIIKPLKMLKEGTKALREGDYSARVSMNLKNEFA
                     ELQDTFNEMAEKIETEIKLREQSEAERKKIIMDISHDLKNPLAIVVGYTELCLKKAES
                     LDKELINHLQIIFKNSKRAGVLLNDLFEFSKLESSEFSMKFHKTDICEYLRQACGDIL
                     PMIEQAGFEYSFDIPEKAVYVMLDVSQMNRVLFNLAENAIRYNQSGTSLSVSLYDDKT
                     NVLIYFSDDGIGIPSHLAKNIFKPFVRIDDSRNSQTGGTGLGLSIAYKIIKSHGGDME
                     LKTDLNKGCTYIITLPKI"
     gene            8450..9673
                     /locus_tag="PKH42_04720"
     CDS             8450..9673
                     /locus_tag="PKH42_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOA19766.1"
                     /translation="MYDSVIISLLSLSLIINIFLLIKQIKNNTNERIISELKIYLKDN
                     NLDLIDSVNRKIAESEQKQLRELLTNKLETVERIDRNSNKLTESFAKFSADTSKTLNE
                     NFISLNERVSQNLDRINLRVEERLNEGFEKTNKTFVNILERLSRIDEAQKKIDSLSTN
                     IVSLQDVLTDKKSRGTFGEVQLNHILSVVFGEKNDKIYEIQKKLENSMIADAVLYIPE
                     PVGMLCIDSKFPLENYQRMIDVNISEAERKQYSRDFKLNVKMHINDISSKYIIPGQTS
                     DQAIMFIPAEAIFAEINAYHQDLLDYAGEKKVWITSPTTLMSVLSTVQVVLRNIEREK
                     YAGIIHEELNKLGKEFKLYKDRWESLSRNIKRVSDDVDKINITSNKIERKFDRISRVE
                     MGCEPEIAEIEYSEI"
     gene            9733..9815
                     /locus_tag="PKH42_04725"
     tRNA            9733..9815
                     /locus_tag="PKH42_04725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9766..9768,aa:Leu,seq:caa)
     gene            9989..10855
                     /gene="rsmI"
                     /locus_tag="PKH42_04730"
     CDS             9989..10855
                     /gene="rsmI"
                     /locus_tag="PKH42_04730"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOA19767.1"
                     /translation="MSKLYVVPTPIGNLGDMTNRAVETLNSCDLILAEDTRHTQKLLN
                     YFNIKTKMISYHKFNEKQRSEEIIHKLLNEDINAALVTDAGTPCISDPGYELVREVRE
                     KGIEVIGLTGASALTTALSISGIETYEFAFYGFLPRKKSELDTALKRVLNNPVKTFVF
                     YESPKRIISLIENIKIHMPNSTLCVCKELTKIHEKTFYGSVDDVLEQLNNHGDVEKGE
                     YVIVGYNNDYKEKGDTDIQVSLHAILFDKLFKNEYSLKEAVNEAALEGIASKNEAYKA
                     SLEIKKIIEKLR"
     gene            10861..11541
                     /locus_tag="PKH42_04735"
     CDS             10861..11541
                     /locus_tag="PKH42_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008910035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOA19768.1"
                     /translation="MIGTIANALAIIVGGIIGLLFKNIIPEKISEALLKAIGLAVIGI
                     GINLMLSGENFTLLIISMVIGTIIGEGIDIEKKLDKIGAFIESKMKNKESNVALGFVA
                     CTLVYCVGSMAIVGSIQSGLTGNHEILFSKAVLDGITAVTFAATMGVGVVFSGISVLV
                     YQGTITMLASIMQSLLNPVVVSEMTAIGGVIIMGIGLNFLISNRMRVGNMLPSIFIPI
                     IYYMILMR"
     gene            11546..12892
                     /locus_tag="PKH42_04740"
     CDS             11546..12892
                     /locus_tag="PKH42_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOA19769.1"
                     /translation="MDRNLVLKPSAIMSICLIFIIGSIILSIPLYFGFLAGIVLTSTV
                     LYKSGYHMREIYETSVSALKDVKRLLIVILFIGATTSIWLASGVVPTIMYYGFTYMEG
                     VNFLLAVFLITSVVSYFLGTAIGTISTVGISLSGIGLSIGIPHNLMVGVLVSGAFIAD
                     KISPLSGLVNLGMTTVNRNYKELFKSMIITLIPAIAATSVIYFIIGSSYIAKTENLIM
                     YKQAIQEGFNTSPALFILPAVILILSISGLDSLKTFSIGIGVGAILSIIFQQESLLSI
                     IKALIFGYRGNTPSEELNRLLLSGGMISMIEAIFIVAGAYLLIKLFEKGNVLVPLMDK
                     LVYGINNKMSLIGRTGLISILMTVATCDQTSGIILPGTILQEKYKELKLDNAVLARTI
                     FDTGVIVAPLMPWNINSFLIKPVMGITAAQYAPFAVLCIVCPLITMLVSYFQYRNS"
     gene            13115..14902
                     /locus_tag="PKH42_04745"
     CDS             13115..14902
                     /locus_tag="PKH42_04745"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOA19770.1"
                     /translation="MKKLTDNSNVLKWVDEIISMTNPSNVVWIDGSEELYEELREEAC
                     STGELVKLNEEIYPGCYYHRSAKDDVARMEDKTFICSRKKEDAGNTNNWKSPEEAYKL
                     GREIFTDSMKGRTMYIIPYSMGPVGSPFSKIGIEITDSIYVVLSMNIMTRVGKEVLDA
                     LGDSNDFVRGVHGKCTLDINKRYIFHFPEDNTIWSCNSGYGGNVLLGKKCFALRIASY
                     QGRMEGWMAEHMLIVGIESPQGEVKYIAGAFPSACGKTNLAMLIPPEIYLNKGYKVWT
                     VGDDIAWLRIKEDGRLWAINPEAGFFGVAPGTNAKSNYNALMSTKKNSIFTNVVLNLD
                     DNTVWWEKLDNNPPENALDWMGNPWNGKTSKENGAHPNSRFTAPAKNCPCISPEWESP
                     EGVPLSAIIFGGRRAKTAPLVYQSRDWQHGTFVGSILASETTAAASGKVGVVRRDPMA
                     MLPFCGYNMGDYFKHWLEMGKKMSNPPKIFHVNWFRTNEEGQFIWPGFGDNFRVIEWM
                     LKRCENKADANEAPIGYLPKPEDINIEGLNLSADTLKSLLSIDTESWLADIKNIEEFY
                     AKIGDTIPQELIEELKRLADNLIKSAALV"
     misc_binding    15258..15487
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15618..16604
                     /locus_tag="PKH42_04750"
     CDS             15618..16604
                     /locus_tag="PKH42_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA19771.1"
                     /translation="MKNSTIKFIAILFLVSLIITGCSANTEANKPAGGKFTIGIAQLV
                     QHPALDASRQGFIDEMEKLGVEVEFIDQNAQADINNAQMIAEKFVKDDVDLIFSIATL
                     TSQAAKKAIEGTKIPLVFTAVTDPVFSELVTALDVTDNNITGVVDAAPIKENLELFKL
                     LKEDIKTVGIIYNIGESNSEVQVEQTKEIAAELGLTIETVGITSINDIPQAVDTISKK
                     VEGLYIITDNMVASAMELVAKLAGEKELLTVSADGSHVDQGILISKGISYYVIGQQSA
                     RIAKQILVDKVDVADIAVQASTEFEKKVNLKTAESLGLAKEHKAFEGAEFVE"
     gene            16601..17479
                     /locus_tag="PKH42_04755"
     CDS             16601..17479
                     /locus_tag="PKH42_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19772.1"
                     /translation="MNTLILTSIEQGLIFSVLAIGVIITFKILDFADMSVEGTFPMGA
                     FIFAKFITTGFSPIMSTLLTFILGTLAGLLTYTLNIKLRIRALLSGILTATILYSANL
                     RLNGRSNIGLTSYDSIFDGFRPVIVLILIVLAVKILMDIFLRTEVGYLLIATGDNETL
                     VKSLGENSNKYKLIGLMLSNGLVSLSGALMAQYQGFSEISMGTGIIVVAIASIIVGDT
                     VLRNSKILKMTTRAIMGALIYKFIGALAIDLGLAPTDLKAINAAIVIIFLSYNTFSPV
                     KMRKARKIKAVEGVKC"
     gene            17473..>18088
                     /locus_tag="PKH42_04760"
     CDS             17473..>18088
                     /locus_tag="PKH42_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA19773.1"
                     /translation="MLDIINLSKTFNQGTDYEIRIFSNFNIHINKGECTAILGANGCG
                     KSTLFNIISGFLMQEKGIIKLNGKNINGLKENERAKFIGRVHQNPSAGVSPSLTILEN
                     ISLSDKKCQKFTLRKLIRKNKIEAYKEILRTLDLGLETKLDTEVKYLSGGQRQSLSLL
                     MATMNKPQLLLLDEHTAALDPKTSRLVMEKTQELIKTQNITTMMI"
BASE COUNT         6442 a         2732 c         3569 g         5345 t
ORIGIN      
        1 tgaataaatt taataaggag aaaacaaatg aaagacccat atgaaattct tggtttgaaa
       61 agaggagcaa caaaggaaga agttaaaggt gcttatagaa agttagcaaa aaagtatcac
      121 cccgacatga atgtgaacaa tcctcttcag gatttggcgg aagaaaagtt caaggaaatt
      181 caatgggctt atgacgaaat aatgagcgga aatgcagacg gtccgggcta tggctcaaca
      241 tataccggca ataattcaaa aagcaccggt gatttgtttt ctgcaagaca atatgttaat
      301 gcagggagat tttatgaagc tttgagtata ctccgaaaaa ctaaagtcag aaatgcagag
      361 tggaattttt taatgggtgt atgctacgtg agtttaggct ccataaatca aggaatgcag
      421 catattcaaa ctgccgtgaa catggagcct tccaacatgg aatatcaaaa ttatttaaat
      481 caaatttaca atatgcagcg cacataccaa caaagggctt acagttacgg aggaggctca
      541 agttcggttg attgctgtgc tcaactgata tgcgctgatt gtctgtgtga atgtttggga
      601 ggggacctga tttcatgctg ctaaaaagca ataatgtggc atacttaggt gtgcttttag
      661 gcttaaatca gttgtttata atactttcat cggtaattga aacaaacacc ataatattaa
      721 tggcagcagc agcattgatt gtaggagttg tggtagttga atttggaggg aaagcaggaa
      781 taatatttta tattgcatca tgtatttcag gattcttttt aacattcaac aaaattgaga
      841 ttattacata catatatttt ttcggaattt acagtattat taaacactat attgaaacaa
      901 aaatattcaa taaatacata tcctatgctg taaaaatagc atcttttaac atttctatct
      961 tattaatgta ctttactgca aaactattta taaacttacc tatccgatgg tggatgataa
     1021 tagcagccca agtattgttt attatctacg actacgccta tacaatattc ataaatcaat
     1081 atataaattt tataaaacca aagttgggaa gatagaagca cttctcattt ttaagattga
     1141 attctgagaa atggttgaca tatctttcgt aaacgagcaa caattttgtg tgacgtaagg
     1201 agtcgaaaaa tgaaaaaacc tgtattatcc attgcttata ggtatagatt atgttaaaat
     1261 taatcgtggt ataagagtag tttataaata ttatatacta tgggtcatct gcacctatta
     1321 tttaaaattg gaggatttat gcttaaaaaa ataaaatatg caattttatt attattacta
     1381 accgtgttat tgacatcttg tactgaatcc gcagatgcag atgcaaatac acccccttcc
     1441 gatgaaacaa atcaggaaat tccggaaata atagaatccg aattatcaga agaagaaaaa
     1501 gcaaaattag agttggaaaa aagaattgct gaaagggaag aattagaaga acaaaggaaa
     1561 gaagagctgg gagaatttta tgttccgctg attcccatag gggaagaaag agaaaaaaag
     1621 agtgttgaag ttaaagcatt gtatgttacc ggtcatacgg caggcaatga tttaaacaag
     1681 gaaaatatcg atgcatatgg tgaatatgtg aaagcacttg ccgaaaataa cactcaaaaa
     1741 gcaagggaat tgtttgacaa ggctgaaaag gccaataaat ttgagcgtat tgtgggaata
     1801 gctcttgcta ctgagataaa cggattggtt ataaatgtta aagatgacaa cggctttata
     1861 acatataaga gcaatattga aattgtcaaa gaaatgaata aggaaacacc tattccgata
     1921 aaagatatag acaatatgct taaaattctg aaggaatatg atttatacat cataggaaga
     1981 attgttgcat ttaaggacaa gaattttgcc gttaagagtc cggagcatgc catacagcta
     2041 aaatccggcg ggatatggct tgaccctaac agcggaaaaa ttccatggat aaatcccttt
     2101 gataaatatg tgtgggatta taatgtggca attgccaagg aagcatcact gctgggtttt
     2161 gatgaaattc aatttgacta cgttcgtttt ccggatggag ctaaaacata taatgctatt
     2221 gcggaatttc cgggaagaga cggcagagat aaagatgaag caatagctga ttttttagaa
     2281 tattctaaaa aagaattaga accttacaat gtaaatttag gtgcagacgt atttggtctc
     2341 gttacaagga catgggatga ttatcctgag gatataggcc agacttggat actgattggt
     2401 aaacatgttg ataatatttg tcccatggtt tacccgagtc attattcaac agggtggtat
     2461 aatttagaaa atcctaatgc tcatccttac ttggttgtaa aaggtgctat ggaagaagca
     2521 atagaaaaaa attcctccat ggaaacacct ccaattatac gaccttggat tcaggacttt
     2581 gactgggatg gaattgcata cggtcctgat aaagtaagag accagataat agcagcaaag
     2641 gagcttgggg tatatgaata tatgatatgg aatccaagca atgtttatga tccatatgca
     2701 ttcatggtta cagaaaagga aaagataacc gcttatccgt taaataaagg agaactggac
     2761 tacagagaaa aatcacctgc tgatgcagca gttaagtatt taaccggtat gcttcaagac
     2821 aggcacagct acacatattt aatgactcct gtttcagaca gagttaatga ttttgatgaa
     2881 tttgtaaaac ttcgggaaga agataatatt aagcttttaa gctacaaggt ttacggctat
     2941 gaaatggatg caaatgacag caataaggcc aatgtaagcc tatcctataa aatcgaagtt
     3001 aaaaacggag atacaacaga aataatagaa aaagacaatg ctaaatggca agcaataaga
     3061 gagcttaaca tctggaaagt aaaagtaaac tgggatattg gaaaaaatgt acactgaatt
     3121 aaaacacatg tacataaaat aaagtacaaa aaataaatca aaaagcttga caagctgaaa
     3181 tttattgtta taatattctt taaatattta aaagcaggta agcttgcaga aggtgaggac
     3241 gatatgaaaa aatacaatgt agcaatattg ggagcaacag gcgcagtagg gcaagaaatg
     3301 ctaaaagtgt tgggagaaag gaattttccc ataaatgatt taaaattgct ggcaagcaaa
     3361 agaagtgcag gagcaatagt tgaattcaac ggggaagaat atgcaatcga agaagcaaca
     3421 gagtcatcct ttgaaaatat agatgttgtg ttgtgtgccg ctgaaaatga tataagcgaa
     3481 aaattgtctc ctgctgctgt aaaagcaggt gcggttgtag ttgataattc aagtgcattc
     3541 agattatatg atgatgttcc cctggtggta cctgaagtta acggagaaga cgttaaaaac
     3601 catcagggaa taattgcaaa cccaaactgc tcaacaacaa tagctctaac ggcaataaat
     3661 gagcttaata agtattctcc aataaaaagg atgatagtta caacatttca ggcagtatcg
     3721 ggtgccggcg ttaacggtat aaaggaactt aatcagcaga ttaaggatat aagtgaagga
     3781 aaagatgtag aaataaatac attccagcat cagatagcat acaatgtaat tccccaaata
     3841 ggaagtttca atgaaatggg gtattcgcag gaagaaatga aattgctgaa tgaaggaaga
     3901 aaaattcttc ataatcctga cttaaaagtt aattgtacat gtgtaagggt tcccgtttac
     3961 agaagccatt ccgaatcaat tacaatagaa acagaaaagg aaataacggt agaaaaagca
     4021 agagagctgt tgtcaaaagc agcaggcgtg aagttaatcg atgatttgga aaacaataaa
     4081 tatccaatgc ctcttgattc atcagaccag gatttaattt atgtaggcag aatcagaaag
     4141 gatataagcg gcgaaaattc attggttctg tggtgctgcg gagaccaaat aagaaaaggt
     4201 gcagctacaa atgcagtgca aatagcagaa gttttggtta aagaaaattt aatttagttt
     4261 ttcccggtgc ctttttccct gtgccaggca ccattgtggt gcctggctcc aatgtatatt
     4321 tataggatat gaaagaatag catccatgct gagaataata ttcctgatag aacccatatt
     4381 gcaggcagca gttccttata ttttttattt tttattgcaa taattatatt tgatataacg
     4441 agtgctgcaa tgaaccatcc gagaaatttc atgtaaggag catatttaag aattgaaaag
     4501 gttacaattc ctttatacat aagcaaacat aagatgacaa acgaaacaat ttggaaaaag
     4561 gaattcaatg caaatgcaaa agaatattta ttgtttttat tactaaactt tttaatcagt
     4621 tctctaataa agatgataaa tgttacgatt ataaaaatta tcggtataat catttggata
     4681 attctatgct gccacaatgg aacttttaca tatgttatgg cagattctga agacataatt
     4741 attttatttt gcacttcctt aaattttagc aatcctaaat ctgacgaata caaatcctca
     4801 tcgattaaag tcatcttttg accattaata taatgcccgt cttttaccgt atctatatat
     4861 atcattttgt taggtataaa gtaacgcaga attttttcgg gagtattgaa gctggaccaa
     4921 ttatttacat ataaaccgcc tatcttaatt gatgagtcgg cattttttat agtgtattct
     4981 tgtttctcgc cataaatcaa gtcagtggct ttattcataa tattattaat ttcatgctca
     5041 ggtacataag tgttaaatga tagaaaaact cccgtatcag cttccgaata aagggctatc
     5101 cttgtataaa aatttaaggt ttctcctttt ttatcataat aaactttatt gtcttgttct
     5161 ttcctgttcc agattagtcc tataccttca aattcctctg acattgtaaa ttgtttttta
     5221 aacagctctg ctttggaatc aggtgataat acacgattat catcaccgtc catcaaccaa
     5281 atcatatatt ttgccatatc ctctgccgtt gacacagccg agccctccgg atataagttc
     5341 atgtaaggct caatagtttc attgccattc ggcgaatatg cttttgaaac ataggcttta
     5401 tcatgcataa atctaaatgt tgtcctattc atatttaaag gctcaaaaat cttctcctcg
     5461 caaaataagg caaaatcctg ctctgtaata cattcaatta catatgcggc aagtgctata
     5521 ccgtaattcg aataagatat aacctcaccc ggcttatata tttgtcttgg caggtactta
     5581 cgtacactta tttcaagagg ttcagtatcg cttacattat aaaaagccat acctgtaacc
     5641 atatcttcaa atcctgcggt atgtgtgaga agctgtctca tagaaacagg ataagtaagt
     5701 tttaaatcta aatccagata aattgatata tcgctatcca tgttgagcaa ttctttttca
     5761 acaagaattt gagctgcaac cgcaacaaag gtttttgata ctgagccgat tcgaaatgca
     5821 attttttctc catcagaagc aatgccgagg ttatcatcag ccaagccata ccctttgcac
     5881 aattcgatat ttccatcctt tacaacagac agtacggcac ctttggattt tccttcatcc
     5941 actatccttt gaaccatctt atccaactga ttgtatgttt ctatatcggc tgccaaaacg
     6001 cacggttgtg cagctgagaa aaaaattatt ataactaata ctaaacttat tgcttttttc
     6061 ataatatcct cctttatttt tgaatttatt ttattttggc agcctgaaat ataaatgtta
     6121 tttaccttaa atttgcctta aattaacaag aaagtattta tcagatatta atattgtgct
     6181 atattgaaaa tataaaaaat tcttaaagag gggataaaag tgaatatatt agtggcagag
     6241 gatgaaatag atattagaaa ccttataaag ataaccttgg aggatgaggg ctattccgta
     6301 attaccgcat gcgacggtat tgaggcgtta aaaaaactga aggaaacacc tgtcgactta
     6361 gccatattag atattatgat gcctcggctt gatggattta acctgcttag aaaaataaga
     6421 gaaaacagca ccattcctgt tatatttctt actgcaagaa cagatgattt ggacaaggta
     6481 ttaggacttg aattaggagc tgatgattac attgccaagc ctttctccat gagtgagctc
     6541 attgcacgag tcagggctca acttaggaga aataatgaat acatgctgcc caaacaagaa
     6601 aataaaacct tgcttcaata tggtgattta acgatagata aagaaagttg ctgtgcttac
     6661 aaaaataatg agcaaataga actcaatgca aaggaataca aactgctctt acatttaatg
     6721 gaaaaccccg agagagtatt tacaaaaaaa cagctctaca atgcagtttg ggatgatgac
     6781 tattactatg atgataatac aatcatggtt catatcagtc atctgcgcaa taaaatagaa
     6841 actgattctc aaaatcccga atatataaag actatacgag gcttaggata taagcttcat
     6901 aaaacaggtg gatataatga aaaataaaat ttcccatcaa ttcctgttaa attatcttac
     6961 attttttctt atgtcattat tagcagcttt atttgcctat ttattgttgt cgtttgcaga
     7021 tgatgtgatt tcaaaaacac ttgtaaaaaa cagataccct tcagaactca tcatgaaaga
     7081 caattatgcc gatattgatg cagaatcagt agttgagcat ggcggtggag tacaggtaat
     7141 aaatgaaaac tatgaagtgg tgtattcaaa aggattagat actcttggaa agaagcagtt
     7201 ttctgtgagt gaatttactg attttcttgt aaaaagcaaa gagaaaggat ttaagtatca
     7261 ttatgatatt ctatataatc cgcaggcaaa gttttggctt attgtaacat tcccgacatc
     7321 aatgcgttta gatatttcct ttgtttacaa taaggaagct gcctcggagg atttaaaaaa
     7381 tgtatccttc gtattcattt ccgtgtttat tttatatttg cttctgcttg ggttgcttgc
     7441 ctttattttc tcaaaaatta catcgataca cataattaaa cccttaaaaa tgttaaagga
     7501 agggactaaa gcattaagag aaggtgatta ttctgcaaga gtttccatga atttaaaaaa
     7561 tgaatttgca gaattgcagg atacttttaa tgaaatggct gaaaaaatcg agaccgaaat
     7621 aaagctgaga gaacaatcgg aagctgaacg caagaaaata attatggata tttctcatga
     7681 tttaaaaaat cctctggcta ttgttgtcgg atatacggag ctatgtctta aaaaagcaga
     7741 gtcactagat aaagagctta ttaatcatct tcaaattatc tttaaaaaca gtaaaagagc
     7801 aggtgttttg ttaaatgatt tgtttgaatt ctctaagttg gaaagctctg aattctccat
     7861 gaaatttcat aaaaccgaca tatgtgaata tttaaggcaa gcatgcggcg atatactgcc
     7921 tatgatagaa caagccggct ttgaatacag ctttgatata cctgaaaagg cggtatatgt
     7981 gatgctggac gtcagccaga tgaaccgtgt actattcaat ttagctgaaa atgcaataag
     8041 atacaatcag tcgggaacca gtttatcagt aagtttatat gatgataaaa caaatgtact
     8101 aatttatttc agtgatgatg gtataggaat tccgtcacat cttgcaaaaa atattttcaa
     8161 accctttgtc aggattgatg actctcgtaa ttctcagacg ggaggaacgg gcttagggct
     8221 gtcaatagca tataaaataa ttaagtcaca tggcggagac atggaattaa aaactgattt
     8281 aaataaagga tgcacttata ttatcactct tccaaaaatt taatatacct ccaaaaaatc
     8341 ctttagagtt tttatgaagt aatctataag attgtaagca tacgagagta atttaagtgg
     8401 acaaatgttc gattttgtag tattattaat aagtaaaggg gaggtgtcca tgtacgattc
     8461 agttattata tcattgcttt ctttatcatt gataatcaat atttttttac ttataaaaca
     8521 aataaaaaac aatacaaacg agcgcataat atcagagctt aaaatttatt taaaggataa
     8581 taatttggat ttaattgatt cagtaaatcg aaaaatcgct gagtctgagc agaagcagct
     8641 gagggaattg ttgacaaaca agctggaaac agttgaaaga atagatagaa actccaataa
     8701 gctgacagaa tcattcgcaa aattcagcgc tgatacatct aaaactctaa atgaaaattt
     8761 tatatctttg aatgaaagag taagccaaaa tcttgacagg ataaacttaa gagttgaaga
     8821 aagattgaac gaagggtttg agaaaaccaa caaaacattt gtcaacatac ttgaaagatt
     8881 gtcaagaata gatgaagcac aaaagaaaat agacagcctt tccacaaata tagtttcctt
     8941 gcaggatgtt ttaaccgata aaaaaagcag aggcactttt ggagaagttc agcttaatca
     9001 tattttgtcg gttgtctttg gggaaaagaa cgataaaata tatgaaatcc aaaaaaaatt
     9061 agaaaattca atgattgccg atgctgtttt atacataccg gagccggtag gaatgctttg
     9121 catagactcg aaatttcctc ttgaaaatta ccagcgcatg attgatgtca atatttctga
     9181 agctgaaaga aaacaatatt caagagactt taaattaaat gtaaagatgc atattaatga
     9241 tatatcttca aaatacataa ttccgggaca aacttcagac caggcaataa tgtttattcc
     9301 ggcagaagca atatttgcgg aaataaacgc atatcatcag gacttgttgg attatgcagg
     9361 cgaaaagaaa gtttggataa cttcaccaac gacattaatg tcagttttgt caacggttca
     9421 ggttgtttta agaaatattg agagagaaaa gtatgcagga ataatacacg aagagcttaa
     9481 taaattagga aaagaattta agctctacaa ggacagatgg gagtctttat ctagaaatat
     9541 taaacgtgtt tcagatgatg tggataagat taacataact tccaacaaaa ttgaaaggaa
     9601 gttcgacaga atatcaaggg tagaaatggg ctgcgagcct gaaatcgcag aaatagaata
     9661 cagcgaaata tgaattgcaa aaaatacttg cattttactt ttgtttatgc tactatctta
     9721 aagtagcatt ttgccgcagt gatggaatgg cagacgtgac ggactcaaaa tccgttggtg
     9781 gcaacaccgt gggggttcaa gtcccttctg cggcataatc aatataaaat cgaccttata
     9841 gtcgatttat ttttttatgt gttaaaatgc gttagtaaag aaaattacac ccaagcgata
     9901 attatgaata ttgttatatt gcatttttaa ctatatattt atagtttttt gtgatataat
     9961 tttttaacta agaaataaag gtgaaaagat gagtaaatta tatgtggtac ctacgcctat
    10021 cgggaattta ggcgatatga caaacagggc agttgaaaca ctgaattctt gtgatttaat
    10081 attagctgaa gataccaggc atactcaaaa gctgttaaat tatttcaata ttaaaacaaa
    10141 aatgatttca tatcataaat ttaatgaaaa gcaaagaagc gaagaaataa tacataagct
    10201 tttgaatgag gatatcaatg cagccttggt aacagatgca ggaacaccgt gtatttcaga
    10261 ccccggatat gaattagtga gggaagtcag ggaaaagggt attgaagtaa taggtttaac
    10321 cggtgcatct gctcttacaa cagccctttc catatctgga attgaaacat acgaatttgc
    10381 attttacgga tttttgcccc gtaaaaaaag cgaactggat actgctttaa aaagagtgct
    10441 taacaatccc gttaaaacat ttgtttttta tgaatctcca aagaggataa taagtttgat
    10501 tgaaaatata aaaattcata tgccgaattc aactctttgt gtttgcaagg aattgacaaa
    10561 gattcatgaa aaaacttttt atggaagtgt ggatgatgta cttgagcagt taaacaatca
    10621 tggtgatgtg gaaaaaggcg aatacgttat tgtaggatat aacaatgatt ataaagaaaa
    10681 gggtgataca gacatacagg taagcctcca tgcaatttta tttgacaaat tattcaaaaa
    10741 tgaatattct ttaaaagaag ccgtaaatga ggcagcatta gaaggaattg cttctaaaaa
    10801 tgaagcgtat aaggcttcgc tggaaataaa aaaaattatt gagaagttaa ggtagggcat
    10861 atgataggca caatcgcaaa tgcacttgca ataatagtag gcggaataat aggactttta
    10921 tttaaaaaca taatacctga aaaaatatcc gaagcacttt taaaggctat cggtctggca
    10981 gttattggca taggcataaa tttgatgcta tccggtgaaa attttacact tttaatcata
    11041 tcgatggtaa taggaacaat aataggtgaa ggcattgata ttgaaaaaaa actggataaa
    11101 atcggagcat ttatcgaaag taaaatgaaa aacaaggaaa gcaatgtagc gcttggattt
    11161 gttgcatgca ccctggttta ctgtgtgggc tccatggcta ttgtgggctc aattcaaagc
    11221 ggattgacgg gaaaccatga aattttattt tcaaaagcag tgcttgacgg cattacagcg
    11281 gttacttttg cggcgacaat gggtgtgggt gtagtttttt ccggcataag cgttcttgtg
    11341 tatcaaggta cgataacaat gcttgcaagc attatgcaat ctttgttaaa ccctgtggta
    11401 gtaagcgaga tgactgctat aggcggtgta ataattatgg gaatcggtct gaattttcta
    11461 atatcaaaca gaatgagggt aggtaatatg ctgccgtcta tatttatacc tataatatac
    11521 tatatgattt tgatgaggta ataaattgga tagaaattta gttttaaaac caagtgctat
    11581 aatgtctata tgtctaattt ttattatcgg ctcaattatt ttatcaatcc ccctatattt
    11641 tggttttttg gcggggattg ttcttacttc tacagttctt tataaaagcg gttaccatat
    11701 gcgggaaatt tacgaaacat ctgtgtctgc attaaaggat gttaaacgtt tattgattgt
    11761 aatattgttt ataggcgcaa ctacttcaat ttggcttgca tcaggtgttg tgcctacaat
    11821 tatgtactat ggttttacat atatggaggg agtaaacttt cttttggcgg tatttctaat
    11881 tacgagtgtt gtttcatatt ttttgggcac ggcaatcgga actataagca ccgttggaat
    11941 atccttaagc ggaatcggat taagtatagg aatacctcat aatttgatgg ttggcgtctt
    12001 ggtttcggga gcttttatag cagataagat atctcctttg tcgggtttgg taaatctcgg
    12061 aatgactacc gtcaacagaa actataaaga actgttcaag agtatgatta taacattgat
    12121 accggctata gcagcaacat cagtaatata ttttattata ggttcaagtt atattgctaa
    12181 aacagaaaat cttataatgt ataaacaggc aattcaagaa ggatttaata catcgccggc
    12241 attatttatt ttacctgcag taattctaat tttatccatt tcgggacttg attcattaaa
    12301 aacattttct atcggtattg gggtaggtgc aatacttagt attatatttc agcaggaatc
    12361 gttattatca attattaagg cgcttatatt cggttatcga ggcaacacac cttccgaaga
    12421 attaaacaga ctcttgctca gcggaggtat gatatcaatg atagaggcaa tattcatagt
    12481 tgccggagca tatttattga ttaaactttt tgaaaagggc aatgtgcttg tgcctttaat
    12541 ggataagctg gtttacggaa taaacaataa aatgagttta ataggcagaa caggactgat
    12601 aagtatattg atgacagtgg caacatgtga tcaaacatcg ggaattatat tacccggcac
    12661 catattgcag gaaaaataca aggagctgaa attggacaat gctgtcttgg caagaaccat
    12721 atttgacacg ggagttattg tggcacctct aatgccttgg aatataaatt catttttaat
    12781 aaaacccgta atgggaatta ctgcagccca atatgctcca tttgctgtat tatgtattgt
    12841 ttgtcccttg attacaatgc tggtttccta ttttcaatat aggaattcat aaccgaaatt
    12901 tgtttcatga ttctcttatt gtactacagt gttacaatta ttttgctttt ttaaaaaata
    12961 cagttttatt ttaagtaaag cataataaaa gttaagaatc ttcgaatttg tttttaatat
    13021 tgaatgtatt atacagtagc tatataatac acttgtgata tagtggttac aataaataaa
    13081 acatagtaac acaaataata tttaggagag tgccatgaaa aaattaacgg acaattcaaa
    13141 tgttttaaag tgggttgacg aaataatttc tatgactaat ccatcgaatg tagtttggat
    13201 tgacggcagt gaagaattat acgaggaact tcgcgaagaa gcatgctcaa caggcgaact
    13261 cgtaaaattg aacgaagaaa tttaccccgg atgctattac catcgctcgg caaaagatga
    13321 cgtagcaaga atggaagaca aaacatttat ttgttccaga aaaaaggaag atgcaggtaa
    13381 tacaaacaat tggaaaagcc ctgaagaggc ttataaattg ggaagagaaa ttttcactga
    13441 ttccatgaag ggaagaacaa tgtatattat tccctattca atggggcctg ttggttcgcc
    13501 cttttcaaag ataggaattg aaatcaccga ttctatttat gtcgttttaa gcatgaatat
    13561 aatgacaaga gttggaaaag aagtacttga tgcattagga gattccaatg atttcgtgag
    13621 aggtgtacac ggaaagtgta cattggatat caacaaaaga tatattttcc attttcccga
    13681 agacaacaca atatggtcat gcaattccgg ttacgggggc aatgttctcc tcggcaaaaa
    13741 atgctttgca cttcgcattg cgtcatacca ggggcgaatg gaaggttgga tggctgagca
    13801 catgctcatt gtaggaatag aaagccctca gggagaagta aaatacatag caggagcatt
    13861 cccgtcagca tgcggcaaaa cgaatttggc tatgcttatt cctcctgaaa tttacttaaa
    13921 caaaggatat aaagtttgga ctgtaggcga tgacatagca tggcttcgaa ttaaagaaga
    13981 cggaagacta tgggctataa atcctgaagc aggctttttc ggagttgcac cgggaacaaa
    14041 tgcaaaatca aattataacg ctcttatgtc tacaaagaaa aattcgattt ttacaaatgt
    14101 tgtattgaat ttggacgata acacggtatg gtgggaaaaa cttgacaaca atcctcctga
    14161 aaatgctctg gactggatgg gaaatccatg gaacgggaaa acatcaaagg aaaacggagc
    14221 acatcctaac tcaagattta cggcaccggc taaaaattgt ccgtgcattt cacctgaatg
    14281 ggaatcaccg gagggagttc ctttatcggc aataatattc ggaggaaggc gtgcaaagac
    14341 tgctcccctt gtttatcagt caagggactg gcagcatggt acttttgtag gctcgatact
    14401 tgcatcggaa actactgcag ctgcatcagg aaaagtcgga gttgtaagaa gggaccccat
    14461 ggccatgctt ccattctgcg gatacaatat gggagattat tttaaacact ggcttgaaat
    14521 gggtaaaaaa atgagcaatc cgccaaaaat tttccatgta aactggttta gaacaaatga
    14581 agaagggcaa tttatttggc cagggtttgg agataatttc agggtaatag aatggatgct
    14641 caaaagatgt gaaaataagg cggatgcaaa tgaggcacct atcggttatc tgccaaaacc
    14701 cgaagacatt aatattgaag gtcttaattt atcagcagac acattgaaat cacttctttc
    14761 aatagacacc gaatcatggc ttgcagatat aaaaaatata gaagagttct atgctaaaat
    14821 cggggataca ataccccaag aattaattga agaacttaaa agattagctg ataatttgat
    14881 caaaagtgca gcattggttt aatgttatac agagcaatca acaatataat aactaaaaat
    14941 atttagcgaa catttgcagg acacgactat aattcttagc gaatgaaggt taaaaatccg
    15001 ttaagcagct tgcattgttt gagcgcagcg agtttgcaag ctgtaggatt tttagccgga
    15061 atgagcttta gaattattcg tggccgaaac cgtgagcgta tatttttagt tatgtttctt
    15121 taaataatcg gacaatctca aataggaata caacagttta cgagggcgta gccctcgtta
    15181 tttttatgat tggtaaaata ttactgaaaa ataattgttg acaaagggat tattataagt
    15241 tattattgct attaatattt aaagcgttga agcagagaaa agtatttcag acgtttacag
    15301 agaattggcg gtggtgagag ccagtaacag aggaatataa tatacactgc tgagcaggtt
    15361 tgctaaaagg aactccgagt aggcgaacac gtgtactcac gttacagggt tagaagttgt
    15421 tagacttcga agagtgatga tttatatcat aattagggtg gtaccgcgga aatctttcgt
    15481 ccctataagc atattttaaa aaagtatgtt tataggaatg aaagattttt tttatgattt
    15541 acctgtaatc atgttttttc gatatcgaac cctggccaag ttcatatcta aaaataaata
    15601 aaccttggag ggacattatg aaaaatagta caattaaatt tatagcaatt ttatttctgg
    15661 tttcgctgat aataaccgga tgttctgcaa atacagaagc aaacaagcct gcaggaggaa
    15721 aatttacaat aggaatagca cagcttgtgc aacatcctgc cttggatgct tcaagacagg
    15781 gatttataga tgagatggaa aagttgggtg tagaagttga atttattgac cagaatgctc
    15841 aggcagacat aaacaatgca caaatgattg cagaaaaatt tgttaaagat gatgtggatt
    15901 taatattttc gattgcaaca cttacatctc aggcagcaaa aaaagcaatt gaaggaacaa
    15961 agataccatt ggtatttact gctgtaacag accctgtttt ctctgaactg gtaacagctt
    16021 tggatgttac agacaacaat attactggag tagttgatgc agctcccata aaggaaaacc
    16081 ttgagttgtt taaactgtta aaggaagata taaagacagt tggaataatt tacaatattg
    16141 gagaatcaaa ctctgaagtt caggttgaac agacaaaaga aattgcagca gagctcggac
    16201 tgacaatcga aacagtcggg attacttcaa ttaatgacat accgcaagca gttgacacca
    16261 tttcaaaaaa ggtggaaggt ctatatataa ttacggataa catggtggca tccgctatgg
    16321 aattagttgc caagttagcc ggagaaaaag aattgttgac tgtatccgcc gatggcagtc
    16381 atgtagacca ggggatactg atttcaaagg gtataagcta ctacgtaata ggtcagcaat
    16441 ctgcaaggat agctaaacaa atattggttg ataaagttga tgttgctgac attgctgttc
    16501 aagcatcaac cgaattcgag aaaaaagtta atttaaaaac agcagaaagt ctgggactgg
    16561 caaaagaaca caaagcattt gaaggagcag aatttgttga atgaatacat taattcttac
    16621 atcaattgaa caaggtttaa ttttttccgt attagcgata ggagttatta ttacatttaa
    16681 aatacttgat tttgcagata tgtccgttga aggaacattc ccgatgggag catttatatt
    16741 tgctaaattc attaccacag gcttttctcc tattatgagc acacttttaa cctttatcct
    16801 gggaactttg gcaggactgt taacatacac attaaacatt aaactaagaa taagagcttt
    16861 gttatcgggg attcttacag caaccatatt atattcggca aacttaagat taaacggcag
    16921 atcaaatata gggctaacaa gctatgattc aatatttgac ggattccgac ccgtaatagt
    16981 gcttatactg atagttcttg cagtaaagat attaatggat atatttttaa gaaccgaagt
    17041 ggggtatttg ttaatagcaa caggagacaa tgaaacattg gttaaatcct tgggagagaa
    17101 ttcaaacaaa tacaaattga taggattaat gctttcaaac ggattagtat cgttaagcgg
    17161 agctcttatg gcacaatacc agggtttttc ggaaatatca atgggaaccg ggataatagt
    17221 ggtggcaata gcttcaataa ttgtgggcga tacagtatta agaaacagca aaatattaaa
    17281 gatgacgaca agggcaatta tgggagcctt aatatataaa ttcattggag ccttagcgat
    17341 tgatttaggc cttgcaccga cagacctaaa agcaataaat gcggcaatag taattatatt
    17401 tttgagctac aatacgttca gccctgtgaa aatgagaaag gcaagaaaaa taaaagccgt
    17461 ggagggagta aaatgttaga tataataaat ttatcgaaga ctttcaacca aggaacagat
    17521 tatgagataa ggatattcag taatttcaac attcatataa acaagggaga atgcacggca
    17581 atattgggag caaacggatg cgggaaaagc accttgttta acataataag cggcttttta
    17641 atgcaggaga aaggtattat aaaattaaac gggaaaaata tcaacggttt aaaagaaaac
    17701 gaaagggcta agtttatagg aagagtgcat cagaatccat cggcaggcgt ttcaccgtca
    17761 ctcacaatat tggaaaatat aagcctgtcg gataagaagt gtcagaaatt tacattaaga
    17821 aaattaatca ggaaaaataa aatcgaagct tataaagaaa ttttaagaac tcttgactta
    17881 ggacttgaaa ccaaattgga tacggaagtg aaataccttt caggagggca aaggcagtca
    17941 ctgtccttgc tgatggcaac aatgaataaa ccgcagcttc ttttgttgga tgagcacaca
    18001 gcagccttag accccaaaac ctcaaggctt gtaatggaaa aaactcagga attaataaaa
    18061 actcaaaata ttacaactat gatgatat
//
LOCUS       DAOPUE010000034        17251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3367_length_17251_cov_9.690975, whole genome shotgun sequence.
ACCESSION   DAOPUE010000034 DAOPUE010000000
VERSION     DAOPUE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_17251_cov_9.690975"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKH42_04765"
     CDS             <1..614
                     /locus_tag="PKH42_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19774.1"
                     /translation="HFEDNIVVLINNEWVLFEKEVSTKSEETYVLPINREAVENVVRD
                     KNSNWTITDEQNKEYQTTFVIKDRKDEPGRIDAGITVTIDSIGNEEERYLAVQVMYPN
                     DYFVKQIQEEQVQNLPLLFDIASEIYGHVDSKALYDEFIKHLDGNENYEIKGVQWNHE
                     VNGSHVFVKMTPLNNGIMYRKCAVFIMNDVSYEKFKDGLILNQ"
     gene            715..1023
                     /locus_tag="PKH42_04770"
     CDS             715..1023
                     /locus_tag="PKH42_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19775.1"
                     /translation="MKNVILSHDDKVRLYSVPDEVADNLDKYCWEFAANWIWKNPNGA
                     KLLQEIRGQKVAIYGSTDFIDYLNEWIFPQQKSVLIEKLNYYDYELPEKYKDYPQYNF
                     "
     gene            complement(1148..2530)
                     /locus_tag="PKH42_04775"
     CDS             complement(1148..2530)
                     /locus_tag="PKH42_04775"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOA19776.1"
                     /translation="MKNTKRLMLMNYSYYKEIEKKLELYAKKGLVLEKMGPYFWTFKK
                     TEPQNLKYTVTYFAEGSVFNPHPTDNQQTYFDYAKAAGWDFVCEYNQMQIFCSSLENP
                     PEFETDEKEKLENIHKCMKKSFVISQLLMLLVFALNLYLRFNILKRNPTDFLSSNIDL
                     ATLLMFISIILYSSYTLINYYMWYNKSKKSVDMGCSIIENFNKTQRYFDIGYLIFLFS
                     LVGYMFIHLLTNTAFGIIALSVAQLPLFALVFWGSITLLKRRKISANANKIISTSLLI
                     LTGVLYLGFIFYSIPRFNFSERSNKPYTTVGEYRLYSDKIPLTCEDLYGHIDFEYYSY
                     EADEEDTPLLHRSHYSQTRPPMKNSPPELEYSIYEPKFEFVKNIVVSDLTKLNEWSDR
                     KLKALDNAVFSTEKAYAFYYESPNGMLFSGEYILVFTDKIIHIDAETPFTDEQIEIIK
                     SKLLSGNSKV"
     gene            complement(2531..2848)
                     /locus_tag="PKH42_04780"
     CDS             complement(2531..2848)
                     /locus_tag="PKH42_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOA19777.1"
                     /translation="MPREKYQTLTEQMFYILLCLQSECCGVDIMKMVSEMTEQRVTVG
                     PGTLYTLLDNFVSQGFITLTNMENRKKSYIITDKGREMLKKEYDRLHKLTEDYERFMK
                     KVI"
     gene            3317..5380
                     /locus_tag="PKH42_04785"
     CDS             3317..5380
                     /locus_tag="PKH42_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19778.1"
                     /translation="MKSKVFLLIVLVVILSSCSFQTDEKVSDKNDLDTIGELNDENTD
                     EELNNVITEEELNSKENHADILDRIINDIKNKLEIYKDIKLADIKKIQDTYYVVVKVN
                     EAEMTDHDSMLWKYDMLPELLLTGNAIDILSMDNYYFVSYRESLQYGTKYTITMYDHY
                     DENYEKVIYEGGFSKFTFSPNNDYMYIQNDNEVIILDKDYEILFKNKLYNNAENIEDG
                     HYVDMQFISWTKDNKKLWVATGYHAYVMTFHLIDIENSTQDTFFTGESYGVSEIALDP
                     DNSCIVYSTKPEFYESSAYEEFNNNQTIVYLYFLNLVTNEKIEISKSVTRGFRPKWVN
                     NKIFEYNHPDGFMRLTFNIDNYYLNSGKENPENEENLWQYVEGITPFVNNKDGNWYYN
                     ETTYKINLKIPSIYHLDSPGVYFYWREDGKGCYISLTNYIYEFDKNTSFVENVKNIYF
                     LNNSYINENIFDNVIIEEGTTDNGYKYVYFADDITDYSNDRVLSYIFVQLDDYAICRL
                     DYALFSEDFGKFDADVIINSITHTLTEGRFRDEFFTVYNDDAVNLVNFKVPYFYIQNY
                     RGEYYYETSEKKGSFYPLDYFIDNKEEITLYIQDTDTEIEYMGEITEGYTKNGYKYSY
                     YVEKETVSYQPYGTEKWEEVTRFITNISVRLDKYSVLKMKYELSEEDFSLFDVNEILD
                     SVQLK"
     gene            5562..7004
                     /locus_tag="PKH42_04790"
     CDS             5562..7004
                     /locus_tag="PKH42_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19779.1"
                     /translation="MKKIYRTKFNVFIVVLICVFLFNIIVYANSAPIYMPDYPSTGIF
                     SVDENTPIEVLKENLLFDFTEEDDDTYSPKAKVIATYEMQNSSNEDVSVYMAFPFIER
                     FSEFSPDEIIITANDEIIPYDTYLGETINNSMNPFQEAENTDFNIEKIIKSITDKKYT
                     ANNFKENERGKLYRFKVSSADDKINFAVSFDFNHEKTKIILNGFNRFERNENNIRVAS
                     GTREEETREIYVLGEDISFDIKGYTSGNLEEETDKFEYEIITEEIDVETFLKNYAAYY
                     LNKYADNLEEMAKGQISEIQLYNLYAESLDKYLDRNLGVCTEGDIMSETWVPRIITLV
                     YSVNFPQNSKKEISVSYKTNGTMDRRETAEPQFTYDYILNPARNWKGFKNLNIKIITP
                     EEAPYIVNSSIELNKEDNNVYTAYSEELPEEDFSFTLYSKEKVTLSDRIEGYLNRTFG
                     YFTPIVIGFVFIITVIVILVVVKTKKRNKE"
     gene            7247..8893
                     /locus_tag="PKH42_04795"
     CDS             7247..8893
                     /locus_tag="PKH42_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOA19780.1"
                     /translation="MKKSRFKKFVLVGLTCTIISTSAIFALADNFDANKAIRLQINNE
                     WVDAQNLYIENDRAMVPLDGIIEELGLKTESDLATNTIKVYDDNLTIILTIGNNTAGV
                     IKVISGEIIEEANNLDVAPKIINNQIFVPIRFVAETFGLQVSWDDTLRAVIIEAASGI
                     SVERPIDFEVLDFQAIMDNDLLKNLYDNNYMTKGIYSLVDGEWMYVLAAAGEKPTGGY
                     DLTIDSITEVTKGTAYIHVTLTSPEEGSMVTQVLTYPTVFVKFNKGDIRDIQWDVTGD
                     VTEPETDEQEKVLIENLVKSFGEKLKMVSLLATEDMVKESIKEYYGEFFSQVLLEKWL
                     SDPAKALGRTTSSPWPERIDIISFEKLSDTEYEVKGNIIEMTSVELTQGGIAASRPAT
                     FIIRNIDGKWLIDDIKIGDYIDLNLLVYDNKEYGFYFTLPASWIGYKVLEDKWEGVSL
                     TESKENQSGPIIYIRHPEWTEKTPRQDIPIMIFTKDQWESLNNGEFSVGAAPVGPSIL
                     GENTSYVFALPARYNYEFLTGFEEVEEILENDSLQAYEVN"
     gene            9117..9899
                     /locus_tag="PKH42_04800"
     CDS             9117..9899
                     /locus_tag="PKH42_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOA19781.1"
                     /translation="MFDNQDDAVNNLLNKEGMTVKDRYLPIDGYTRVEYEEGSFAEFL
                     RNQKLKPYGEKVLYYDGRQKPAKGIYDSVFDVDIGNRDLHQCADAIMLLRAEYLYSQG
                     LYDKISFNFVSGFKAEYKKWMEGNRIDVQGNNAEYHKTAEPSNTYENFRKYMDMVFAY
                     SGTLSLEKELISVSIDNMSIGDVFIVGGSPGHAVIVVDMAVNSLGEKIFLLAQSYMPA
                     QQTQLLINPMDEDISPWYSLKGKEKLITPEWTFELDTLKSWY"
     gene            10244..10405
                     /locus_tag="PKH42_04805"
     CDS             10244..10405
                     /locus_tag="PKH42_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19782.1"
                     /translation="MFISNTTDFYSTKLTKIDDVTFKIEYYDNSKGEKFEDIFKWDGK
                     RFIIAQSIN"
     gene            10481..10942
                     /locus_tag="PKH42_04810"
     CDS             10481..10942
                     /locus_tag="PKH42_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19783.1"
                     /translation="MKKKICYMICIVFITLLLSNIFQYYCLNNFHFGVNVSAQEREYP
                     EYTDKIMGFGGYVNNKTIFPIKIREINPIGSRGMEYFATLNTTWGFSEIEQNKMGNYE
                     DIENKVILPFKEYDIGVFHKFNGDYVVNADAYELKYSVLGINFKKVIIKTH"
     gene            11069..12157
                     /locus_tag="PKH42_04815"
     CDS             11069..12157
                     /locus_tag="PKH42_04815"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOA19784.1"
                     /translation="MLIKEIENLSGMDRANIGAELPQVYYPWRRYLARAFDGLIYAIF
                     WAAFLAFVFHVNVVGRNNAEKFLDGFITIIMMLFLEPSLLHLFGTTPGKAIFGLKIEN
                     PDGGRLSYTDGVVRTWGVLSKGMGFNIPIYSIIRLFKSYNLCIANETQPWDESISYTI
                     KDTKWYRGVIYVGATLATFAAFFMISSAQQLPPNKGDINIAEFAENYNYYAKLYKIDF
                     GNEYLDEDGKWAEKETDGTVIYINLTEKPEYKFTIENGYVTGVSFDIEMKNHDYMISS
                     YDKHMFLASLALAGAQNEMGLFSKIPKRIARSIENNILNDYQFVEAGIAFNCDIEHSG
                     YNKITNILVPSENTKENYFSLKFSMNKE"
     gene            12194..13861
                     /locus_tag="PKH42_04820"
     CDS             12194..13861
                     /locus_tag="PKH42_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOA19785.1"
                     /translation="MKTRIFIILILTLLMTVAPFVTEDVYGAEYVKGQSIVAEDQEKA
                     SRGLEFPGLDITWLDIGDSHIQSYSQMDIFSEGIFTLRLSGQTICFDTTGKIIAVGDY
                     NGIHEFSDGMAKVYKYIPRDEPDVPGRLMAPPGQMEGFIDSEGNEVIPLGMHSNMGDM
                     FHEGFTTIGGYEENKGFINKTGEIVIPQIYKDAGNFSEGLAAVQDIETELWGYINKDG
                     DIVIPMLYDEAKPFREEVAYVVKNGMAGYIDKEGNIIIDFMLMPETDKYVDNSFYGGL
                     AVAWDSSGKYGYINKNGDYVLPVKYKEANPFIGEAVYVVIENQNYPNGYGSSFLINRD
                     GERLTPLWQYGRYAGEYMRDGLIRALSTYGPSPKEFIVMLNKYGAEVIPASLNIQYLS
                     SFNEGYALIIADNNGGTAIGLVELPENIDAYKDRKLIRVFIDDKLLNFEDTDPIIENS
                     RTLVPMRAIFEALGAEVEWDDTNKTAIARKDNTTISLKIDDNTAYINNEPVELDVPAK
                     IKNSRTLVPVRFIAESLNADVKWNDEGRAVLIKTSGIDNTAVDDYQE"
     gene            13916..14842
                     /locus_tag="PKH42_04825"
     CDS             13916..14842
                     /locus_tag="PKH42_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOA19786.1"
                     /translation="MMKKYLDINPKVKTALEQGKPVVALESTIISHGMPYPKNVETAL
                     NVEKIIRDNGAVPATIAILSGKLKVGLTEKEIEYLGKATNVIKASRRDIPYIVSKKLD
                     GATTVASTMIVASLAGIKVFATGGIGGVHRGATETFDISADLEELANTNVAVVCAGAK
                     SILDIGLTLEYLETHGVPVVGYQTEEMPAFYTRKSGYKVDYKLDTPEEIAKMLKAKWD
                     LGLKGGMVVANPIEEQYQMDYDIITDAINKAVKQADENGIKGKDTTPFLLAKVKEITG
                     GDSLESNIMLVYNNASLGAKIAVELSKIYEIE"
     gene            15091..16671
                     /locus_tag="PKH42_04830"
     CDS             15091..16671
                     /locus_tag="PKH42_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19787.1"
                     /translation="MSIVNTFIHETDFYRILFENLPQAIQINQIVFNETGEPVDYLLL
                     DVNRTAEKYMGLKREEIVGKRIKEIYSYIEKEWFIRYGEAVKTGKFDRLEIYIEALGI
                     WFDVQVIPLGSQNKFAILFQDITELKNKEASEKKALNDRKKIEIEKSHYMKIAEERAA
                     WLQTIMDLMPAGVWISDYSGKVIMVNQEAINMYRGSSSMTDIPKEYTSYRLFLPDTDE
                     PVTFEPYPLKEALVGVMLDFERFDGTRGTQVASTEALRDKDGNIINYVAVAMDITSLR
                     QAERALAESEKNALDLVEKLRQADHNKNVFINMLSHELRNPLASIIMGLELLEKAPSD
                     GKQAYMAMDIVKRQAKLLTDLVDDLLDVTRITHNKVYLKKDTLEINNLIGKVVQDYQP
                     QFIDKNVKLEFKSTIPVYIEADSSRLAQAIGNLLHNAVKFTNEKDLVIVSAALDTNSS
                     EAVIAVEDTGRGIDQTDQPNLFEPFMQVDKSLDRKLGGLGLGLTIVKRVVELHGGRVE
                     VFSEGVGKGSKFTIRLPI"
     gene            16695..17081
                     /locus_tag="PKH42_04835"
     CDS             16695..17081
                     /locus_tag="PKH42_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA19788.1"
                     /translation="MSNDILNILIIEDNKDLVDIMCEMLVLSGYRALSAKNGTEGIAK
                     ARENRPDVIICDIGLPDMSGYEVAKIIRKDSELKDTYLIASSGYAQPEDVERSRETGF
                     NIHLAKPISFNILGKVLKGVKLKCMQ"
BASE COUNT         6287 a         2353 c         3380 g         5231 t
ORIGIN      
        1 cacattttga agataatatt gttgttttga ttaataatga atgggttctt tttgaaaaag
       61 aagttagtac taagtctgag gagacatatg tgttacccat taacagagaa gctgtagaaa
      121 atgtggtacg ggataaaaat tcgaattgga ccattactga tgaacaaaac aaagagtatc
      181 aaactacatt tgttataaag gatagaaaag acgaacccgg acggatagat gcaggtatca
      241 cggttaccat tgattcaatc ggtaatgaag aagaaagata tttagcagtt caggttatgt
      301 accctaatga ttattttgtt aaacaaattc aagaagagca agttcagaat ttacctttat
      361 tatttgacat tgcttcagag atatatggac atgtcgattc aaaagctttg tatgatgaat
      421 ttataaagca tttagatggc aacgaaaatt atgaaattaa aggtgtgcag tggaatcatg
      481 aagtaaatgg cagccatgtg tttgttaaaa tgacaccact taacaatggg ataatgtata
      541 gaaagtgtgc tgttttcata atgaatgatg tatcttatga aaaatttaag gatggattaa
      601 ttctaaatca atagaatatt agttacataa tgcaaatgtt acgaatcagt aaataattac
      661 gaactaacaa ttatttatat ataatatcgt taaataggaa tttggagggt atatatgaaa
      721 aatgttattc tcagtcatga tgataaagta agattatatt ctgttccaga tgaggtggca
      781 gataacttag acaagtattg ttgggagttt gctgctaatt ggatttggaa aaacccaaat
      841 ggagcaaaac tgttgcagga aataagaggg cagaaagtag caatttatgg gtcaactgat
      901 tttattgatt atttgaatga gtggatattt ccacaacaga agtcggtact aattgaaaaa
      961 ctgaattact acgattatga attacccgaa aagtataaag actatccaca atacaacttt
     1021 tgagatatac aaattccaat ttgtagagag gagttagcat aattttttca gttgttatgt
     1081 cgtaatgttc aaattatgcg aatcaaccaa atataaaaaa atgcctaaag cgttggcagg
     1141 taatctatta tactttactg ttcccactaa gtaatttgct tttaattatt tcaatttgct
     1201 cgtctgtgaa aggtgtttct gcatcaatat gaatgatttt atcagtaaaa accaatatat
     1261 attcaccgct aaacaacatc ccatttgggc tttcgtaata aaaagcatag gctttttcgg
     1321 tggaaaaaac agcgttgtca agggctttaa gttttctgtc tgaccactca ttaagctttg
     1381 ttaaatcgga tacaacaata ttcttgacaa attcaaattt aggctcatat atactatatt
     1441 caagttccgg tggagaattc ttcattgggg gacgggtctg ggaataatgg cttctatgaa
     1501 gcagaggggt gtcttcttca tctgcttcat aagaatagta ttcaaaatca atatgcccat
     1561 ataaatcctc acaagtaagt gggatcttgt cgctataaag gcgatattct ccaacggtgg
     1621 tgtatggttt atttgacctt tctgagaaat taaaccgagg gatagaataa aatataaatc
     1681 caagatacaa aacgccggtt agaataagaa gtgaagttga gattatttta tttgcgtttg
     1741 cagagatttt gcgtcttttc agcaatgtaa ttgagcccca aaaaacaaga gcaaacagtg
     1801 gcaactgcgc cacagaaaga gcaattatgc caaaagcggt gttagttaac agatggataa
     1861 acatataccc aacaagtgaa aacaggaata taagatagcc tatatcaaaa tatctttggg
     1921 ttttattgaa attttcaata atagagcagc ccatatcaac agattttttg gatttattgt
     1981 accacatata gtaatttatt agggtatagg atgaatagag aataatgcta ataaacatta
     2041 gcaaggtagc taaatctata ttgcttgaca gaaaatccgt gggattccgc ttcaagatgt
     2101 taaatcgtaa ataaagattt aatgcaaaaa ctagaagcat taaaagttgt gatatcacaa
     2161 agcttttctt catacatttg tgaatatttt caagcttttc tttctcgtcc gtttcaaatt
     2221 ccggagggtt ctcaagggaa gaacaaaata tttgcatttg attatactcg caaacaaaat
     2281 cccatcctgc tgcctttgcg tagtcaaaat aggtctgctg attgtcggtt gggtgaggat
     2341 taaaaaccga gccctcggca aaatatgtga cggtgtattt aaggttttga ggttcagttt
     2401 tcttaaatgt ccaaaaataa ggtcccattt tttctaaaac aagtcctttt ttcgcataga
     2461 gctccagctt tttctcgatc tctttgtagt aactataatt cataagcatg agtctttttg
     2521 tatttttcat ttatatcacc tttttcataa atctttcgta atcctctgta agtttgtgaa
     2581 gccggtcata ctcttttttt agcatttctc gacccttatc tgtaataatg taactctttt
     2641 ttcggttttc catattggtt agcgtgatga aaccttgaga tacaaagttg tcaagcagtg
     2701 tatacagggt tccggggcct accgtcaccc gctgttcagt catttctgat accatcttca
     2761 taatatccac cccacaacac tcactttgca gacataacag tatatagaac atctgctctg
     2821 ttaatgtttg atatttctct ctcggcattt agatcacctc cgcaaatatc gaatatcaat
     2881 aattcatatt tccagtatac atcgaatatc gatattgtca atagcttata taggatcata
     2941 aatatattta aaaatttaat tttaaaatag tgggtatggc tgaactgcca tatggggaac
     3001 atttcttgtt ttaattctac gaagaagata ttagaagaga aataatctct gattatactc
     3061 aggagaacaa aaatgagtga ccaaatatat tgttatccca attcggatgt gcctattaat
     3121 aagttttaat tgattcacag cgtctaaagg tagtattatt ttcatgcacg gataaattaa
     3181 tgaggaatct ggtatgtggt tttattataa tattcaactg ctgcaacaaa gtgacttgat
     3241 acttctattg tatttttgtg gaacaaattg ggtaacgaaa tcgtctattt ataatgaata
     3301 ttgcatggag gcaagtatga aaagcaaagt ttttctatta atagttttag ttgttatatt
     3361 aagctcttgt tcatttcaaa ccgatgaaaa ggtgagtgat aaaaatgatt tagatactat
     3421 tggagaatta aatgatgaaa atacagatga agagctgaat aatgtaatta cagaagaaga
     3481 actgaatagt aaagaaaatc atgcagatat tttggacaga ataattaatg atataaaaaa
     3541 taaattagaa atatataaag atattaaatt agcagacatt aaaaaaatac aagatactta
     3601 ttatgtagtt gtaaaagtaa atgaagctga aatgacagac catgattcta tgttgtggaa
     3661 atacgacatg ttaccggaat tgctgcttac aggtaatgca attgatatat tatcgatgga
     3721 caattattat ttcgtaagtt atcgtgaaag cttgcaatac ggcactaaat acactattac
     3781 catgtatgac cactatgatg aaaattatga aaaagttatt tatgaagggg gtttttcaaa
     3841 attcactttc agcccaaaca acgactacat gtatattcaa aatgataatg aggtaataat
     3901 actcgataaa gattatgaaa ttttatttaa aaataagttg tataacaatg ctgaaaatat
     3961 agaagatggt cactatgtag atatgcaatt tatcagctgg acaaaagata ataaaaaact
     4021 atgggttgca acgggttatc atgcttatgt aatgacattc catctcatag atatcgaaaa
     4081 ttcaactcaa gatacatttt ttaccggtga aagctacgga gtatcagaaa ttgctttaga
     4141 tcctgataac agctgcattg tttacagtac aaaaccggaa ttttatgaaa gcagcgcata
     4201 tgaagaattt aacaataatc aaacaatagt ttatctgtat tttcttaatt tagtaaccaa
     4261 tgagaaaatt gagatttcca aatcggtaac aagaggcttc agacctaaat gggtcaacaa
     4321 taaaatattt gaatataatc atcctgacgg atttatgaga ttaaccttta atattgataa
     4381 ctattattta aattcgggta aggagaaccc tgaaaatgag gaaaatttat ggcagtatgt
     4441 agaaggcata acaccttttg taaataataa agatggcaat tggtattata atgaaaccac
     4501 atacaaaatt aatcttaaaa taccatccat atatcattta gactctcccg gggtttattt
     4561 ttactggagg gaagatggta agggctgtta tatatcattg acaaattata tatatgaatt
     4621 tgataagaat acatcttttg ttgaaaatgt aaagaatata tattttctta ataattctta
     4681 tataaatgaa aacatatttg ataatgtaat aattgaagaa ggaactactg ataatggcta
     4741 taaatatgtt tattttgcgg atgatattac tgattattcc aatgacaggg tgctatccta
     4801 tatatttgtt cagttagatg attatgcaat atgcagatta gattatgctt tgttttctga
     4861 ggattttgga aaatttgatg ctgatgtaat aattaattcg attacccata cattaacaga
     4921 gggacgtttc agggatgaat ttttcactgt ctataatgat gacgcagtca atttagttaa
     4981 ttttaaagtt ccctactttt atatccaaaa ttatcgcggt gaatactatt acgaaacttc
     5041 tgaaaagaaa ggcagctttt atcctttgga ttatttcata gataataagg aagaaattac
     5101 actttacatt caagataccg atactgaaat tgaatatatg ggagaaatta cagaaggtta
     5161 tacaaaaaat ggatataaat attcatacta tgtagaaaaa gaaacagtat cttatcagcc
     5221 ttatggaaca gaaaaatggg aggaagttac gcgatttata acaaatatat ccgtcagatt
     5281 agataaatat tcagtactaa aaatgaaata tgaactatca gaagaagatt tcagtttatt
     5341 tgatgtaaat gaaattttag attctgtaca actcaaataa tgcttatata aataacaaaa
     5401 ggctcctaaa tatattttta aggaatttga attttaaaga agatataggg ggtatcggct
     5461 gagtaccatg tggggaacat ttcttatttt aattcgtctg aataggtgac agctagttat
     5521 gaataaagga aaaaattaat tatataaagg caggaaaagc tatgaaaaaa atctatcgca
     5581 ctaaatttaa tgtatttatt gtagtgctta tttgcgtttt tttgtttaac ataattgtat
     5641 atgctaattc agcaccaatt tatatgccgg actatccttc aacaggaata ttttcggtag
     5701 atgaaaacac tccgattgaa gtgttaaagg aaaatttgct gtttgatttt accgaagaag
     5761 atgacgatac ctattctcca aaggccaagg tcattgcaac ctatgaaatg caaaattctt
     5821 caaatgagga tgtttcagta tatatggcat ttcctttcat tgaaagattc agcgagtttt
     5881 cacctgatga aattattatc actgcaaatg atgaaattat tccctatgat acctatttag
     5941 gtgaaacaat taacaattca atgaatcctt ttcaggaagc tgaaaatact gactttaata
     6001 ttgagaaaat tataaaatct attacagata aaaagtatac cgctaacaat tttaaagaaa
     6061 atgaaagagg taagctttac aggtttaagg tatcttctgc tgatgacaaa ataaatttcg
     6121 ccgtgagctt tgattttaat catgaaaaaa ccaaaattat tttaaatggt tttaatcgct
     6181 ttgaacgaaa tgaaaataat atcagagttg cttccggcac aagagaagaa gagacaagag
     6241 agatctatgt attaggtgaa gatattagtt ttgatattaa aggttataca agcggaaatc
     6301 ttgaggaaga aaccgataaa tttgaatatg aaataataac agaagaaata gatgtagaaa
     6361 cattcctgaa aaattatgcg gcttattatt tgaataaata tgctgataac cttgaggaga
     6421 tggcaaaggg acaaatatca gaaatccagt tgtataacct ttacgcagag tctttggata
     6481 aatacttaga caggaattta ggagtttgta ccgaaggtga tattatgtct gaaacctggg
     6541 tgccacgcat aattacatta gtctattctg tgaattttcc ccagaattct aaaaaggaaa
     6601 taagtgtgag ctacaaaacg aatggaacga tggataggcg ggaaacggca gaacctcaat
     6661 ttacatatga ttatatttta aatccggcaa ggaactggaa aggatttaaa aacttaaata
     6721 ttaaaatcat aactccggag gaagctcctt atattgttaa cagcagcatt gaattaaata
     6781 aagaggataa taatgtttat actgcttatt cagaagaatt accggaggaa gacttttcct
     6841 tcacgcttta ctcaaaggaa aaagtcacat tatcagacag aattgaagga tatttaaaca
     6901 gaactttcgg gtatttcaca cctatagtaa taggttttgt atttataatt actgttattg
     6961 ttattcttgt tgttgttaaa acaaagaaaa gaaataaaga ataaataaaa gagtttattg
     7021 accaccgggg acggttctca ccggacaagg ttcaatacaa gaatcctaat aacaactaaa
     7081 aaattaatat gatttaaggg tggctgatat agatgaaatc catatcggcc atttctataa
     7141 taaatttaaa atttctagct tgagtaagat ttaaggtttg aggaacaaat aagataaatt
     7201 tgcgtcttat ttatatatca atttgtttgt tacaggagga aaagacatga aaaaatcaag
     7261 gtttaagaaa tttgttcttg taggattaac ttgcacaatc atatccacaa gtgccatatt
     7321 tgcattagcg gacaattttg atgcaaacaa agcaataagg ttgcaaataa acaatgaatg
     7381 ggtggatgca caaaatcttt atattgaaaa tgaccgtgca atggttccat tagacggtat
     7441 catagaagaa ttagggttaa agactgaaag tgatttagca acaaatacaa taaaagttta
     7501 tgacgataat ttaacaatca ttttaacaat aggtaacaat actgccgggg ttataaaggt
     7561 tattagcggt gaaattattg aggaagctaa taacttggat gtagcaccta aaataatcaa
     7621 taatcaaata tttgttccca tacgctttgt tgctgaaaca tttggtttgc aggtaagttg
     7681 ggatgataca ttaagggcgg ttataataga agccgcatcc ggtattagcg ttgaaagacc
     7741 tattgatttc gaggttttgg attttcaggc aattatggat aatgacttgc tgaaaaattt
     7801 atatgataat aattatatga caaaaggtat ttattcattg gttgacggag aatggatgta
     7861 tgtattggca gctgcagggg agaaaccgac tggaggatat gatttgacca tagacagtat
     7921 tacggaagtt acaaagggta cggcatatat tcatgttact ttgacttctc ccgaagaagg
     7981 cagcatggtg acccaggtgt taacttatcc cacagttttt gtaaagttca acaagggtga
     8041 cataagagat attcaatggg atgtaacagg tgatgttact gaaccggaaa ctgatgaaca
     8101 agaaaaagtg ctgatagaaa atttagttaa aagttttgga gaaaagctga aaatggtttc
     8161 ccttttggca acggaggaca tggtaaaaga aagtataaaa gaatactacg gggaattttt
     8221 ttcacaggta cttcttgaaa agtggctaag tgatccggca aaggctctcg gacgaacaac
     8281 ttccagccca tggccggaac gtatagatat tatatcgttc gaaaaattgt ctgatactga
     8341 gtatgaagta aaaggaaata taattgaaat gaccagcgtt gaattgacac aagggggtat
     8401 agctgccagc agacctgcaa catttataat aagaaatata gatggaaaat ggctaataga
     8461 tgatataaaa ataggggatt atattgacct taatttatta gtatatgata ataaagaata
     8521 cgggttttat ttcaccttgc cagcaagctg gataggatac aaggttttag aagataaatg
     8581 ggaaggtgta tcattaacag aatcaaaaga aaaccaatcc ggtccgataa tttatataag
     8641 gcatccggag tggaccgaaa aaacccccag acaggatata cctatcatga tatttaccaa
     8701 ggaccagtgg gaatccctca ataacgggga attcagcgtg ggtgcagcac ctgtcggccc
     8761 aagcatatta ggtgagaata cgagctatgt ttttgccttg cctgcaagat acaattatga
     8821 atttttgact ggatttgaag aagtagagga gatattggaa aatgactccc ttcaggcata
     8881 tgaagtaaac taattctttt gagcatttca aatcatgagc atatatacta taattattat
     8941 acaaagagca aactacattt atgagcatcc caaattgctg gcaaaaacta ttttaataat
     9001 atggagagac tgtgatggtg tccataagaa gattatgcat ttatggttta ttaataggtt
     9061 tgttaataac aggatacaaa aatgtcggat tggattcgac tgaagtacct tttaatatgt
     9121 ttgataatca agacgatgct gtaaataacc tccttaacaa ggaaggaatg actgttaaag
     9181 acagatattt gccaatagat ggatacacaa gggttgaata tgaagaaggt agctttgcgg
     9241 aatttttgag gaatcaaaag ctcaaaccct atggcgaaaa ggttttatat tatgacggta
     9301 gacaaaagcc ggcaaaagga atttatgata gtgtatttga tgtagatatc ggcaacagag
     9361 acctccatca gtgtgcggat gctattatgc ttttaagggc agagtatctt tattcacaag
     9421 gactatatga taaaataagc tttaattttg tatcaggatt taaggcagaa tataagaagt
     9481 ggatggaagg taacagaatt gatgtacagg gaaacaatgc agagtatcat aagactgcag
     9541 agccttcaaa tacatatgaa aatttcagaa aatatatgga tatggttttt gcatattccg
     9601 gaactctttc tttggaaaaa gagctgataa gtgtaagtat tgataatatg agtataggcg
     9661 atgtgtttat agttggcgga agccccggcc atgccgtaat agttgttgac atggcagtta
     9721 attcattagg tgaaaaaata tttttactgg cacagtccta tatgcccgcc cagcaaactc
     9781 aacttcttat aaatccgatg gatgaggata ttagtccatg gtattcatta aagggaaaag
     9841 aaaaattaat aacacctgaa tggacttttg aacttgatac acttaaaagc tggtattagt
     9901 agataagtaa atatctttag ttttattaaa gggaactgta attgaaatga aaagattgaa
     9961 atagcaaaat caataactaa aggattcagc ccgaaatgga ttagtaataa aataattgaa
    10021 tacaacgacc ctgatggttt tataaggtta actttcaata ttgatgacta ttatttaaat
    10081 aaagctaagg aaatggtgat actgtcaaat aatgatttat tcccgcttaa cggagaacat
    10141 cagtttttga agctaaaaat ggtcgagtgg cagagaatat aaactgttta cattaaggaa
    10201 ggatgggacg gtagaagaat taactgttaa ggatcattat tcattgttta taagcaatac
    10261 tacggatttc tactcaacaa aacttacgaa aatagatgat gttacattta aaatagaata
    10321 ttatgataat tcaaaaggcg aaaaatttga agatattttt aagtgggacg gtaagagatt
    10381 tattattgca caatcaataa attaacatat gatatgcatt attattttaa caactcatca
    10441 ttgttaacat tcaaattata gaattattca tggaggaaat atgaaaaaga aaatctgtta
    10501 tatgatatgt attgttttta ttactctttt gttaagtaat atatttcaat actattgttt
    10561 aaacaatttt cattttggag ttaacgtatc agcccaagaa agggaatatc ctgaatatac
    10621 tgataaaata atgggatttg gcgggtatgt aaataataaa acaatttttc ctattaaaat
    10681 cagggaaatt aatcctatag gaagtagggg aatggaatat tttgcaactc ttaatacaac
    10741 ttggggtttt tcagaaattg aacaaaataa aatgggtaac tatgaagata ttgagaataa
    10801 agtaattttg ccatttaaag aatacgatat aggtgttttc cataaattca atggggatta
    10861 tgtagtaaat gccgatgcat atgagttaaa atattcagta ttgggtataa attttaaaaa
    10921 agttattatt aaaacacatt aatatcaaat tgttttaatt atatttccat aattactaca
    10981 gttaataccg gataatctga aaaagtattt gactgtgaac agacttcata gtgtattatt
    11041 aaggcatcaa attgcaaggg agaatgatat gctgataaaa gaaattgaaa atttgtccgg
    11101 gatggatagg gcaaatattg gcgctgagtt gccgcaggtt tattatccat ggcggagata
    11161 tttagccagg gctttcgacg gtcttatata cgcgatattt tgggcggctt ttcttgcatt
    11221 tgtgtttcat gtaaacgtgg tgggtagaaa caatgcagag aaatttttag atggattcat
    11281 aactattata atgatgctgt ttttagagcc gtccttgctc catttatttg gaactacacc
    11341 agggaaggca atctttggac ttaaaataga aaaccctgac ggcggtcgtc tttcttatac
    11401 agatggagta gtgaggacct ggggcgtatt aagcaaaggg atgggattta atattccaat
    11461 ctatagtata attcgccttt tcaaaagtta caatctatgt atagcgaatg aaacacagcc
    11521 ttgggatgaa tcaatatctt acactattaa ggatacgaaa tggtatagag gcgtgattta
    11581 tgtcggtgca actttggcaa catttgctgc tttttttatg atatcctctg cacagcaact
    11641 tcctcccaac aaaggagata taaatatagc agaatttgca gagaattaca actattatgc
    11701 aaagctgtac aaaattgatt ttggtaatga atacttggat gaagacggga aatgggcaga
    11761 gaaggaaact gacgggactg taatttatat caatttaact gaaaagccgg aatataaatt
    11821 tacgatagaa aacggatatg tgacaggtgt atcatttgac attgaaatga aaaaccatga
    11881 ttatatgatt agttcatatg ataagcatat gtttttggca tcccttgctt tagcaggggc
    11941 acagaatgaa atggggctat tttcaaaaat cccgaaacga atagcaagaa gtatagagaa
    12001 taatattcta aatgactacc aatttgttga agccggaata gcatttaatt gcgatataga
    12061 gcattcaggc tacaataaga taacaaatat attagtgccg tcagaaaata cgaaagaaaa
    12121 ttatttcagt ttaaaatttt caatgaataa agaataataa tatttgaaga gtaatactat
    12181 aggaggaaat acaatgaaaa ccaggatttt tatcatacta attttaactc tgctaatgac
    12241 agttgcacct tttgttacag aagatgtata tggagctgaa tatgtaaaag ggcagtcaat
    12301 tgttgcggaa gaccaagaaa aagcaagcag gggacttgaa ttcccgggac ttgatatcac
    12361 ttggcttgat attggagact cacatataca gtcttattca cagatggata tattcagtga
    12421 agggatcttt actctaagat tatcaggaca aactatatgt tttgacacaa ccggtaagat
    12481 aatagctgtt ggtgattaca atggaataca tgaattttct gatggtatgg caaaagtata
    12541 taaatacata ccacgagatg aacctgatgt tccggggaga cttatggcac ctccaggtca
    12601 aatggaaggt ttcatagaca gtgaaggaaa tgaagtaata cctcttggta tgcatagtaa
    12661 tatgggagat atgtttcatg aagggtttac gactattggc ggttatgaag aaaataaagg
    12721 atttattaat aaaacgggag aaatagtaat tcctcaaata tataaagatg caggaaattt
    12781 ctcagaaggt cttgctgctg tgcaagatat tgagacagag ctttgggggt acataaataa
    12841 agatggcgat attgttatac ctatgctata tgatgaagct aagccattta gagaagaagt
    12901 tgcctatgtg gtcaaaaacg gtatggcagg ttacatagac aaagaaggaa acattataat
    12961 tgattttatg ctaatgcctg aaaccgacaa atatgttgac aacagtttct atggaggtct
    13021 tgcagttgcc tgggacagtt caggaaaata cgggtatata aataaaaatg gagactatgt
    13081 attacctgta aaatacaaag aagcaaatcc gtttatagga gaagctgtct atgttgttat
    13141 agaaaatcaa aattatccaa atggctacgg ctcaagtttt ctaattaata gagatggtga
    13201 aaggttgact cccctttggc aatatggtag atacgcagga gaatacatga gagatggtct
    13261 cataagagca ttgtcaacct atggtccaag cccaaaagaa ttcatagtca tgctaaataa
    13321 atatggtgct gaagtaattc cggcatcgtt aaacatacaa tatttaagtt cttttaatga
    13381 aggctatgct cttattatag ctgacaataa tggaggtaca gctataggct tggtagaatt
    13441 accggaaaac attgatgctt ataaggacag aaaactaatc agagtattta tagatgacaa
    13501 gcttctaaat tttgaggata cagatccaat tatagaaaac tcaagaaccc ttgtgccgat
    13561 gagagcaata tttgaagctc ttggagcgga agttgagtgg gatgatacaa ataaaacagc
    13621 tattgctagg aaagataata caaccatatc tttaaaaatt gacgataata cagcatatat
    13681 taacaatgag ccggtggagt tagatgttcc tgcaaaaatt aaaaattcaa gaacattagt
    13741 acctgttaga ttcattgcag aaagcttaaa tgcagatgta aaatggaatg atgaaggaag
    13801 agccgttctt atcaaaacat cgggcataga taacactgca gttgatgatt accaagaata
    13861 aattctagaa accggtggaa tagatggaat gttggaggga tgaaggagtt gaattatgat
    13921 gaaaaaatat ttagatatta atccgaaagt aaaaacagca ttagaacagg gaaaacctgt
    13981 tgtagcatta gaatcaacaa ttatatcaca cggtatgcca tatcctaaga atgttgaaac
    14041 agcacttaat gtagaaaaga ttatacgaga taacggggcc gtgcctgcca caatagcaat
    14101 tttaagcggt aaactgaagg tcggactcac tgaaaaagaa atagaatact tgggaaaagc
    14161 aacaaatgta attaaagcat caagaagaga cattccatac atcgtttcca aaaaattaga
    14221 cggggctaca actgtcgctt cgacaatgat tgtagcttct cttgcaggaa ttaaagtgtt
    14281 tgcaacgggg ggtatcggcg gtgttcatag aggagcaact gaaacatttg acatttctgc
    14341 agaccttgaa gaattagcaa atacaaatgt ggcggttgta tgcgcgggag caaaatccat
    14401 tttggatata ggtttaactt tggaatactt agaaactcat ggagttcccg tagtaggata
    14461 tcaaacagaa gaaatgccgg cattttatac cagaaaaagc ggctacaaag ttgattataa
    14521 gcttgatact cctgaagaaa ttgcaaaaat gctaaaagca aaatgggacc ttggacttaa
    14581 agggggtatg gtagtagcaa accctataga agaacaatat cagatggatt atgatataat
    14641 tacggatgca attaataagg ctgttaaaca agctgatgaa aacggtataa agggaaaaga
    14701 tactactccg tttttgctgg caaaagtaaa ggagataacc ggcggcgaca gccttgaatc
    14761 aaatattatg ttagtataca acaatgcctc acttggggca aaaatagctg ttgaactctc
    14821 taaaatatat gaaattgaat aattgttgta ctgtcatatt gaggaatcaa aagctgaagt
    14881 catatggtga aagtctatat tgtattaact gtttattggt tcttaactgg ttatttatta
    14941 agaaatatta aagaaaattc ataaaaatat aacaaaaatt actaaaataa gacaaaattc
    15001 ccaatcttct tagtgtataa tatatgaaat aacataatta ttttattgca attaacctat
    15061 aatccgagaa ccatgttagg aggtatttaa atgagcatag ttaatacctt tattcatgaa
    15121 acagactttt atcgaatatt atttgaaaat ttaccgcaag caattcaaat caaccagata
    15181 gtctttaatg aaacggggga accggttgat tatttgcttt tagacgtaaa tcgtaccgct
    15241 gaaaagtata tggggttaaa acgggaagaa attgtaggaa aaagaattaa agaaatctat
    15301 tcttatattg aaaaagaatg gtttatacga tatggtgaag ccgtaaaaac gggtaaattt
    15361 gataggttag aaatatacat tgaagcactt ggtatatggt ttgatgttca agtgattccg
    15421 cttggaagtc agaacaagtt tgcgatttta tttcaggata taacagaatt gaaaaataaa
    15481 gaagcatcag aaaaaaaagc ccttaacgac cgcaaaaaaa tcgagataga aaaaagtcat
    15541 tatatgaaaa ttgcagaaga aagagctgct tggcttcaaa ccataatgga tttaatgcct
    15601 gcaggcgttt ggatttcgga ttatagcgga aaagtgatta tggtaaatca agaagcgata
    15661 aatatgtatc ggggcagttc ttcaatgaca gatattccta aagaatacac gtcatataga
    15721 ttatttctgc ccgataccga cgaaccggta acttttgaac catatccact taaagaagcg
    15781 ctggttggtg tgatgttgga ttttgaacgg tttgacggta cgagaggcac tcaggttgct
    15841 tcaaccgaag ctctccgtga taaggacggg aatattatca attatgtggc tgttgcaatg
    15901 gacataacat cgctaagaca agctgaaaga gctcttgcgg aaagtgaaaa aaatgcatta
    15961 gatttagtgg agaaacttcg tcaggctgac cataataaga atgtttttat caatatgctt
    16021 tctcacgaat tgagaaatcc gcttgcatca ataattatgg ggctggagct cttggaaaag
    16081 gcaccttctg acgggaaaca agcttatatg gcgatggata tcgtcaaacg tcaagcaaaa
    16141 ctccttactg atttggttga cgatctatta gatgtaaccc gcattacaca caataaggtt
    16201 tatttaaaaa aagatacttt agaaatcaac aatcttatag gcaaagtagt tcaagattac
    16261 cagcctcaat ttattgataa aaatgtaaag ttggaattta aatcgaccat accagtgtat
    16321 atagaagcag attcttcacg attagctcaa gcaattggca acctgctgca caacgctgta
    16381 aagttcacaa atgaaaagga tttggtaatt gtttctgcag cactggacac caatagcagt
    16441 gaagctgtaa tcgctgttga agataccgga cggggtatag accagacaga tcagccaaat
    16501 ctgtttgaac cattcatgca ggtggacaaa tcattggatc gaaaacttgg aggacttgga
    16561 cttggtttga ctattgtcaa aagagtagtg gaacttcatg gcggcagagt agaagtattt
    16621 agcgaagggg taggaaaagg ttctaaattc acaataaggc tacctattta aataaatatc
    16681 ggaggatatt tgctatgtca aatgatatac taaatatttt aataattgaa gataataaag
    16741 atttggttga tataatgtgt gagatgcttg ttttatcagg ttacagagca ttatcggcta
    16801 agaacggaac tgaaggtatt gctaaagcaa gggaaaatag accggatgta ataatatgtg
    16861 acataggtct tccggatatg agcggatacg aggttgcaaa aataatccga aaggactctg
    16921 aattgaaaga tacatatctt attgcttctt ccggctatgc tcagcctgaa gatgttgagc
    16981 gttcaaggga aacgggattt aacatacatt tggctaaacc tataagcttt aacatattgg
    17041 gaaaagtact taaaggtgta aaattaaaat gcatgcagtg aacagataat attatatttt
    17101 aagagcagag tgccgtttga aacaaggaga agaaaagtct ttaactaata taatgaaact
    17161 gtatgtaaag ctttaatagt aaagaacact aaccaggaag aaacaggtga tttattgata
    17221 aatatttctg acaaaataaa gaaaaaatta g
//
LOCUS       DAOPUE010000035        15750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3797_length_15750_cov_11.532781, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000035 DAOPUE010000000
VERSION     DAOPUE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3797_length_15750_cov_11.532781"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="PKH42_04840"
     CDS             <1..932
                     /locus_tag="PKH42_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA19789.1"
                     /translation="NINDMIQIKNFIEEKKPSSAAIVGSGFIGLEMCESFKSLGLDVT
                     IIARSKVSKGIDNDLSLYIENHLREKGVNVYTNTKTAEINDHGVVLGDGTIIKADLVL
                     LATGAKANVTLAKNMGVEIGSTGAIKVDKQMRTNIEDVYSCGDCIEMYSAINGAPVYR
                     PLGSTANKTGTIAGSNITGMKEEFTGILGTAIYRVFDLTVGMTGFSEEEAVKSGYDVV
                     AVTDKRANKPEYMGGRPIIIKSVAEKETGRLLGAQMIGYEGVDRRLDVFAALITLNGR
                     AEDLMHLDLAYAPPYSTPRDPLYYTGVKLRAKL"
     gene            complement(956..1504)
                     /locus_tag="PKH42_04845"
     CDS             complement(956..1504)
                     /locus_tag="PKH42_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOA19790.1"
                     /translation="MNKEQTLSVRDITQTGIFTALTAAGAFISIPIGPVPITLQSFFV
                     LLSGIILGSKKAVFSQITYLLLGLIGFPVFAGFSGGIQSIFKPSFGFIIGYVAAAYAV
                     GRLTEEKTVTQYPWAAVLMGTLIIYTFGLPYMYYILNIMLHNNFSIIKILQSGMFAFI
                     PGDVFKAVVAVLLAKRLKSAAG"
     gene            complement(1566..2537)
                     /locus_tag="PKH42_04850"
     CDS             complement(1566..2537)
                     /locus_tag="PKH42_04850"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOA19791.1"
                     /translation="MAVKNEILHILESNKGKTFSGRELADSLGVSRTAVWKAINSLKD
                     DGYFIETSAKGYSLSETSDILSAEGIRLYLNEEFKNIPIAVYKTIESTNTEAKLLAVR
                     DAGQGTTIIAEEQTKGRGRFGRDFFSPSDSGIYMSIILRPELNIKNSVLVTTAAAVAV
                     CNAVEKLTNASARIKWVNDIFIDNKKVCGILTEAVTNFESGIMDSVVVGIGVNVKTKT
                     EDFPLELQNTAGSIFNNEDNSVRNQLAAEIINNVLNVTKNLEDRGFIQEYKNRSMILG
                     EHILYKKYNDWHEGYASDIDDYGGLIVYTGDGQKITLNSGEVSIKRN"
     gene            3169..4080
                     /locus_tag="PKH42_04855"
     CDS             3169..4080
                     /locus_tag="PKH42_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOA19792.1"
                     /translation="MKLPSNVTLCEVGLRDGLQNEPKILTTSEKLKFAEGLINAGFKV
                     IELGSFMNPKAVPQMADTDELFKSLNNINGVELRALTANKKGVERAINCGCRKIKLNV
                     SASKGHNLANINKTPEETVAGFAENVDLAVKNGLEVSGSISMPFASPWEGIIPVKDVE
                     NIVEAYLRAGIREISLSDASGMAVPNQVYDMCISMRKKYPDVKWWLHFHNTRGMAMAN
                     ILASMEAGITNFDSALGGFGGCPFVPGAAGNVSTEDVVHMMDEMGIETGIDILKIIEM
                     AGSLKAVLGHEGDSYILKAGRSKDLFK"
     gene            4159..5631
                     /locus_tag="PKH42_04860"
     CDS             4159..5631
                     /locus_tag="PKH42_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOA19793.1"
                     /translation="MSRMSSMDEAYAKLNQDQIDEIEEKFAKADAAQKIFEKWNQEDI
                     DRTIRAIAWKVANTQTFKELVAMSIEESKMGDPVSRENKKFKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYAKPVGVIACVVPATNPDLTPAGNAIYALKARNAIIFSPHPRAVKTG
                     GRTIELMREGLRQVGAPEDLIQIMGLGKARINKSMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAYGVGAGNATMVWDETAMDEIKQAAHNTMLSKTSDFGSGCSADGNVIMHESI
                     FDKGVNALVEAGGYLLNEKEQESIKRIMWDEECHRLPDSVAQSPQTMAKLAGFEIPED
                     RKFLIAMGTGSYTPDYKDVWCREKLTTLLAVHKYEGEFQNAVNIVKAIHEVGGKGHSV
                     GIYSYDEDHIHKLALEAQVGRIMVRQPQSKANAGAFTNGMPMTSSIGCGTWGGNATSE
                     NVNLRHYMNVTWVSKEIPEDRPSDEELFGEFYVPEREGKK"
     gene            5645..7462
                     /locus_tag="PKH42_04865"
     CDS             5645..7462
                     /locus_tag="PKH42_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOA19794.1"
                     /translation="MKRLVSEQMVEYLERRNAEYIFGLCGHTVIAFLDALSKSEKLKY
                     ISFRNEQLAAMAADGYARIKKKAGVVLCHLGPGLANTVTGVANASFDSIPMVVIAGDV
                     PRYYFGKHPHQEVNLHSDASQYEILKPIVKRAWRIDDPEALPEILDKAFRLAESGRPG
                     PVLISVPMDVFSVKIDEELFTRTFKDSQETIKPALAPSAAKAIARKLIEAKNPVIHAG
                     GGVLLAGASKELEELVDFLDIPVSRTLMGQGCISDTHPLMLGQTGFWGLEFTHRLTAN
                     ADLILGLGTRFAEADSSSWYPGVTFDMNKTDFLQIDIDPAEIGRNYPVSIGAIADLKL
                     ALGQILEEVKILLPEGIDRPELKEMIKTNRSEFKESNVEISNDGRFPMTPQRILKDVK
                     AVIPEDTIIFTDVGWNKNGVAQQFDITIPGTIHHSSGLATMGFGGSALIGGKLAAPDK
                     VVLTLVGDGGFGVNPGALATAVEQNIPVVWVVMNNSAFGTIAGLEYSNYETKFGTVFT
                     TPDGKPYSPNWAEVAKAYGVEAVKIESADDFKPELEKAIKSNKPYLLDVPMENIAVPT
                     PGSWNINDIYNPKPGIVNGKLVRNEKGEYMPPDHSTSHK"
     gene            7487..8308
                     /locus_tag="PKH42_04870"
     CDS             7487..8308
                     /locus_tag="PKH42_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOA19795.1"
                     /translation="MSRKFSLAYLTIPGTNPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFQFDLDRNLFKDIRNALEHNDIKLMDIELARVREDLKVEDYESAFAAGAELGATDV
                     LSSVWTKDKEFAYREFAKICDLAAKYDLRVNLEFVTFSGIPALDGALELLDKVDRPNA
                     YLMVDTLHAHRSMVGPKDLEKVDRKKFGFIHLCDGPLEIPSLEDPSMIGVAREGRLYT
                     GEGEIDIKGMLLAMPDNPISIELPNSKEMAARGAAGHAARCLVTAKEYFAKNQIE"
     gene            8333..9208
                     /gene="aroE"
                     /locus_tag="PKH42_04875"
     CDS             8333..9208
                     /gene="aroE"
                     /locus_tag="PKH42_04875"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016459772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOA19796.1"
                     /translation="MNININTRMIALLGKPLSQSYAARMQNAAYKAAGIDMLYFYSEV
                     ENDHLPDVVNGIRYMSFAGFAVTKPNKVEIMKYVDEKDPLCEKMNASNTVVILPDGRL
                     KAYNTDGIGFIRALKEEMPNINIKESTFFCIGAGGAGRAVSSVLAYYGAKKIYIANRT
                     LSKAQVLVDDINKNFAPVAELVDLNDEAVLKDKIRQSDVIMNNTGLGMVTKLEETPIP
                     KEFLSSGQLCFDATYNPAKTRFLIEAEEMGCSIMNGLGMSLYQGAEQIELWSGKDAPI
                     EVMRQELMNILSEKK"
     gene            9250..9828
                     /locus_tag="PKH42_04880"
     CDS             9250..9828
                     /locus_tag="PKH42_04880"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOA19797.1"
                     /translation="MSAIKWLDEHFEEAIMVVLLALISCIMMAQIVARNLFNSMTWPE
                     EFCRYCYIWTVFLSLGYTVKKGNMLKVGLVMDLLPTKVRRAIEILVNLIILAICVVFF
                     RYAITYTGIIHKTGQISPAMGIPMWMMYLCTVTGFGLASIRTAQQVVSNIRNFNVKAE
                     TTLEATLKEAIEEVETTGIKIDDDLKNSGGDE"
     gene            9828..11111
                     /locus_tag="PKH42_04885"
     CDS             9828..11111
                     /locus_tag="PKH42_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOA19798.1"
                     /translation="MALWIFIVFMVALVFAVPIAMSMVLGALTPLFLGGTGSSIQQLI
                     ANSFSGSDSTPILAVPLFILGGVLMAEGGISRRLFNFFAYFVGGFTGGLPCAVILTCL
                     FYGAISGSGPATTAAVGSMTIPFLVEMGYNKTWAAGMIATSGGLGVIIPPSIPFVLYS
                     LATGVSTGALFLGGVLPGIAIGLGMMVYAVYYCRKKGEDREKIDKKMKELRDKGLFKL
                     FKESFWALLSPVIVLGGIYSGYVTPTEAACISVFYSLFVSLVIYKSFTFKEIVPFLAK
                     AVKTYAPLCFVLAFAIAFGRVLALAKAPVIIEGFILDNFTTSFTVLTGIVIVFLLLGM
                     VMDTGPAIVILAPILLPAVQALGVHPVHFGVIMVCCLSIGLATPPFGLDLFVAGTLSD
                     TPPMTVAKKAMPFIIAFGIVLFVITYVPWLSLALL"
     gene            11155..12228
                     /locus_tag="PKH42_04890"
     CDS             11155..12228
                     /locus_tag="PKH42_04890"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOA19799.1"
                     /translation="MKKLLIVLLIATMVLLPACTQQAAPEPAPPVEEEPEPSEEEPSG
                     IDALEPVVLKGADNAGVGAAAQLYGELVAQKVKDITGGKLTVDYFPNSQLGNDQELQG
                     QMLAGDIDFVICQTAQTVSFVPEVAIFDLPMVFAKYDAATIDYALNKSDFTEKINQAY
                     QAKNMYCLHFLQGGTFRETTSNKNIQSIDDFNGLKIRTMENKNHMAFWQAMGAAPTPL
                     PWPEVYISLQQGLVHAQENATDTCVGANLQEVQDYLIMTHHILYCNQFLINAEKFDSL
                     DPLYQEALQQAIDEAAVEIEAQLTQINADNKQTLIDGGMELVEFDASFIDQVLDKAKG
                     VYESVGNDIGQDYVDSLLEALEK"
     gene            12317..13489
                     /locus_tag="PKH42_04895"
     CDS             12317..13489
                     /locus_tag="PKH42_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA19800.1"
                     /translation="MDTVRAGLAGAGYAAHLHCEAYKRVSGVNVRLKTIVDIDIEKAE
                     EIRKKYNFENAVKSFDELLKDDEIDLIDIVTPPYLHKDMIIRAVNAGKHVICEKPLTG
                     YFGKAGDSEPIGLMVSKEKMFNDVIRELDEIKDAVKKSGKHFMYAENFVYAPNVQKTA
                     EIIRSKKSRILFMKGEESLRGSSSPLAGRWDKTGGGSFIRVGCHPLSGILWLKQEEAK
                     ARNTEIKIKSVSADMGCVSKNLSDLEKKHLTLAPVDVEDIAAVTVTFSDESKALIIAA
                     DNCLGGTRNYIDVYCNNGSMLCNITPTDILQTYFLDEEGMDDVYISEMLPSKLGWNKV
                     FIVDEVLRGYTSELQDFMECASMDREPLSSIDLAYDAIKTIYAAYWSAEEGRRINF"
     gene            complement(13612..14490)
                     /locus_tag="PKH42_04900"
     CDS             complement(13612..14490)
                     /locus_tag="PKH42_04900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOA19801.1"
                     /translation="MTLQQLYYFRVLAKVQHYTKAAEILLIAQPSLSYSISQLENELN
                     VKLFERNGRSIKLSYAGEQFLKYVEKSLDTLDEGTMMLKTLADSSTGEVSLGYIYSMS
                     SKFIPEMIKKFYENDSNKNIKFSLVQNLSEQLLKDLKSNKIDLAICPKPDDGFKSVKI
                     FEQPLYLIIPKSHPFSHKKCIAVSDLANEPFVALSKSSSLRSTVDDIFARSNFTPNII
                     FEAEECNAVISFVSLNLGVSIVPEIPSMYKHINTVKICKMPLVREIYLCWTDKYSSPA
                     AVKIRDFIINNFSITK"
     gene            14859..>15750
                     /locus_tag="PKH42_04905"
     CDS             14859..>15750
                     /locus_tag="PKH42_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA19802.1"
                     /translation="MESAKKYKYMGFILLQSVIYGFGNPLTKIAFESITPFWSLAIRF
                     TTAFLVFMLFFGKRIIESLKSVEPKVYLPASICMAAAYITCNLALNWTSATNVGFLMS
                     LPVIFAPVLALFLYKVKYRLIHIPIQIVVVIGLYLLCFDGEKLVFGKGEVTALITALS
                     IAGALVFGEESLKQLDAVAVSAAQAGITALISLPCALIFESIEVLSRVTLPAWLVVLY
                     LAIFCTNIAYMLQNTALKHISAAAVSMLQCTQPILTAIISYFILREKLSTLGVIGAVI
                     IIMCIIAENIITAKEQAPSEN"
BASE COUNT         4991 a         2791 c         3437 g         4531 t
ORIGIN      
        1 gaaatataaa tgatatgatt caaataaaaa attttatcga agaaaagaaa ccaagctcgg
       61 ctgcaattgt aggcagcggg tttataggcc ttgaaatgtg cgaaagtttc aaatccttag
      121 ggctggatgt tacaattatt gcaaggtcaa aagtttcaaa gggaatcgac aatgacttgt
      181 ccctgtatat tgaaaatcat ttaagagaaa agggagtcaa tgtttacaca aatacaaaga
      241 ctgcggagat aaatgaccat ggagttgttt taggtgacgg gaccatcatc aaagcagatt
      301 tggtgcttct agccacaggg gctaaagcaa atgtaaccct tgcgaaaaat atgggagttg
      361 aaattggctc aacaggagct ataaaggttg ataagcagat gagaaccaat attgaggatg
      421 tatacagctg cggagactgt atagaaatgt actccgccat aaacggggca cctgtttaca
      481 gacctttagg ctctactgca aacaagaccg gtacaattgc aggaagcaat attacaggaa
      541 tgaaggaaga gttcacagga attctcggga ccgccatata cagggttttt gaccttactg
      601 tcggaatgac cggtttttca gaagaagagg cagtaaagtc aggatatgat gttgttgctg
      661 taactgacaa aagagcaaac aaacccgaat atatgggagg caggcctatt ataataaaat
      721 ctgttgccga aaaagaaaca ggcagattgc taggtgcaca gatgattggc tatgaaggag
      781 ttgacaggag acttgatgtt tttgcagcct taattacctt aaacggcagg gctgaagact
      841 taatgcacct tgatctggcg tatgcgccgc cttattcgac tccaagagat ccactttact
      901 acacaggagt aaaactaaga gcaaaactat agaatttaac cgtccccaaa atgttttaac
      961 ctgccgcaga tttgagcctt ttagctagaa gcactgcgac cactgcctta aaaacatctc
     1021 ccggtatgaa tgcaaacata cctgattgga ggattttaat aatactgaaa ttattgtgga
     1081 gcataatatt taaaatatag tacatatagg gaagaccaaa ggtataaata attaaggttc
     1141 ccattaaaac tgccgcccat ggatattggg tgacagtttt ttcttccgtt aatctgccta
     1201 cagcataggc tgctgccaca tatccgatta tgaagccgaa gcttggctta aatatacttt
     1261 gtattcctcc cgagaaacct gcaaatacgg ggaagcctat aagccccagg agcaagtatg
     1321 ttatctgaga aaacacagct ttctttgagc ctaatattat tcctgacaat agtacgaaaa
     1381 atgactgcag tgtaataggc acgggaccta taggaatgct gataaatgcg cctgctgccg
     1441 tcaatgccgt aaaaattcct gtttgagtta tatctcttac gctgagtgtt tgctccttgt
     1501 tcattcctgc cctcgtctct tcttaatgtc tgccctctgt tcttatttct taataatagc
     1561 tttcttcaat tcctcttaat agaaacttcc cccgaattta atgtaatttt ttgcccatcc
     1621 cctgtataga caattaaacc gccataatcg tcaatatctg aagcataacc ctcatgccag
     1681 tcattgtact ttttatataa aatgtgctct cccagtatca tagaccggtt cttgtattct
     1741 tgtataaaac ctctgtcttc caaattcttt gtaacattta gtacattatt aatgatttct
     1801 gctgccagct ggtttcttac agaattatct tcattgttaa atattgagcc tgctgtattt
     1861 tgtaattcca aggggaagtc ttcggtcttt gtttttacgt ttacgcctat tccaaccaca
     1921 acgctgtcca taataccgct ttcaaagttg gtcactgcct ctgtaagtat gccgcacact
     1981 tttttattat caataaatat gtcatttacc cacttaatac gggcagaggc atttgttaat
     2041 ttctctacag cattacagac tgccacagct gccgcagttg ttaccaggac tgaatttttt
     2101 atatttaatt caggtcttag aataatactc atataaatcc ctgaatcaga aggcgagaaa
     2161 aagtctcttc caaatctgcc ccttcccttt gtttgttctt ctgcaataat tgttgttcct
     2221 tgcccggcat cccgtacagc taatagcttg gcttcagtat ttgtggactc gattgttttg
     2281 taaacagcta ttggaatatt tttaaattct tcatttaaat ataaccttat tccttcagca
     2341 gataatatat ccgaggtttc agacagagaa taccccttgg cggatgtttc tataaaatat
     2401 ccatcatcct ttaatgaatt aattgctttc caaactgctg tccttgaaac tcccaggcta
     2461 tctgccagtt cccggcctga aaaggtcttg cccttattag actccaatat atgtaatatt
     2521 tcatttttta ctgccataaa ccacctcaaa catcaatctg tgcaaaacta agattgtaat
     2581 tattacgaat aactatagca tcaaagacat tatatgttat ctgaacgtaa gtgaaaatct
     2641 caatagactt gatctataaa agtattgtaa ttaacattta tattattgtc aaccaaataa
     2701 ttattttggt taacaataat aatcaattag atacactata ttttctggta gtatttattt
     2761 tagatactaa tgtatgacat aacactacaa atttctgcat gttttaaaat accatttaat
     2821 aattaattgt tatatttatt ttataagcat ataatgttac aaagaatttt tgcagcctaa
     2881 aaatgggtga atatacaaag ctgttttctt attaatcctg catgaaattg ttaatgcttt
     2941 acatttaaaa cgatataatt agactattac tggtcatgga tccagtgaat ttcttataac
     3001 aatgcaaata acctataata attcacttca tgacagaatt atcagcaaca ggtaataatg
     3061 attcatagaa agtctctatc attaccatta aaaacaggta tttgacttgc atatattgct
     3121 ggtttataat tattataata aagtttatac tcgaaaggag tggccagttt gaagcttccg
     3181 tcaaatgtaa ccctatgcga ggttggtttg agagatgggt tgcaaaatga acctaaaatt
     3241 ttaactacat cagaaaaact aaaatttgcg gagggtttaa taaatgcagg ctttaaggtc
     3301 attgaactgg gttccttcat gaacccgaag gcagttccgc agatggctga cacagatgaa
     3361 ctttttaaaa gtcttaataa tattaacgga gtggagcttc gcgcccttac agcaaacaaa
     3421 aaaggggtgg agagggcaat aaattgcggc tgtcgtaaaa ttaagctgaa tgtttctgcc
     3481 agcaaaggac ataatcttgc aaacataaat aaaacaccag aggaaaccgt ggcaggcttt
     3541 gctgaaaatg ttgaccttgc tgtgaaaaac gggcttgaag tttctggctc catatccatg
     3601 cctttcgcat ctccctggga agggattatt ccggttaaag atgtggaaaa tattgttgaa
     3661 gcttatttaa gagcagggat tagagaaatt tctctttctg atgcttcagg aatggctgtg
     3721 ccaaaccagg tgtatgacat gtgcatttca atgagaaaaa aatatccgga tgtcaaatgg
     3781 tggctgcatt ttcataatac cagaggtatg gccatggcta atattcttgc ttccatggaa
     3841 gccggaataa cgaattttga ttctgccctg ggaggttttg gaggatgtcc gtttgttccc
     3901 ggagcggcag gtaatgtatc tacggaagat gttgttcaca tgatggatga aatgggaata
     3961 gaaacgggaa tcgatatact gaaaattatt gaaatggcag gaagtttaaa ggccgtgcta
     4021 ggtcatgagg gtgattctta tattcttaag gcaggcaggt caaaggattt gttcaaataa
     4081 aaatatctat cataatgaaa tcgttttact tatgacaaaa ccccgtccac ttgtcatttg
     4141 taagaaatag gaggaagaat gagcagaatg agttcaatgg atgaggctta tgcaaagctt
     4201 aatcaggacc agattgatga aatagaagaa aagtttgcaa aagccgatgc cgcacagaaa
     4261 atattcgaga agtggaatca ggaagatatt gacaggacta tcagggcaat agcctggaag
     4321 gtagccaaca cccagacatt taaagaatta gttgcaatga gcattgaaga aagcaagatg
     4381 ggagatcccg taagccggga aaataaaaaa ttcaaaataa gaggggtcct ccgtgatgct
     4441 ctcagacaga aaagcgttgg cataattgaa gaaatccctg aaaagggcat cgttaagtat
     4501 gcaaaaccgg ttggtgtaat agcatgtgtt gtacctgcga caaatcctga cttgacacct
     4561 gcggggaatg caatatatgc tctaaaagca agaaatgcaa taatattttc gcctcatccc
     4621 agggcagtta agacaggcgg cagaacaatt gaattgatga gagagggttt aagacaagta
     4681 ggggcaccgg aagatttaat tcaaataatg ggattaggga aagcaagaat taataaatcc
     4741 atgtcggaag ctttgaatac aaaatgcgat ttggtaattg caaccggcgg ccagggtgta
     4801 gtaagaagag catatcattc cggcacaccg gcatatggag taggagcagg aaatgccacc
     4861 atggtatggg atgaaacagc aatggatgaa ataaaacaag ccgcacacaa tactatgctt
     4921 tccaaaacat cagacttcgg gtcaggatgc tctgcagacg gcaatgttat catgcatgag
     4981 agcatttttg acaagggtgt aaatgcctta gtggaagcag gcggatatct attgaatgaa
     5041 aaagagcagg aaagcataaa aagaattatg tgggatgagg aatgccacag attgccggat
     5101 tcagttgccc aatcgcctca aacaatggcg aagcttgcag gatttgaaat acctgaggac
     5161 aggaaatttc ttatagcaat gggaaccgga agctacacac cggattacaa agatgtatgg
     5221 tgccgggaaa aattaacaac acttttggct gtccataaat atgaagggga gtttcaaaat
     5281 gcagttaata tagttaaagc cattcatgaa gtgggaggaa agggtcacag cgtgggcata
     5341 tattcatacg atgaagacca tattcacaag cttgccctgg aagcccaagt gggcagaata
     5401 atggtgagac agcctcaatc aaaagcaaat gccggagcct tcacaaatgg aatgcccatg
     5461 acatcaagca taggatgcgg cacctgggga ggtaatgcaa catcagaaaa tgtcaacctc
     5521 cgccattata tgaatgttac ctgggtttca aaggaaatac cggaagacag acccagtgat
     5581 gaggaattgt tcggtgaatt ctacgtacct gaaagagaag ggaaaaaata aaggaggtat
     5641 ttatttgaaa agattagtgt cagaacagat ggtagaatat cttgaaagac gtaatgcaga
     5701 atatatattt ggtttatgcg gccataccgt aatagccttt ttggatgctt tgtcaaaaag
     5761 tgaaaaacta aaatatatat cattcaggaa tgagcagctt gctgccatgg ctgcagacgg
     5821 ctatgcgaga atcaaaaaaa aggcgggggt tgtactctgc catttgggcc ccggcctggc
     5881 aaacactgtg accggtgtag ccaatgcatc ttttgattct atacccatgg ttgttatagc
     5941 aggggatgtg ccaagatatt actttggaaa acatcctcat caggaggtga atcttcattc
     6001 ggatgcttct caatatgaaa ttttaaaacc aatagtgaaa agagcctgga gaattgatga
     6061 tccggaagca ttgcctgaaa ttcttgataa ggctttcaga cttgctgaaa gcgggagacc
     6121 agggcctgtt ctcatatcag ttcccatgga tgtattttct gtgaagatag atgaggaatt
     6181 gttcacccga acatttaagg acagtcagga aaccatcaag cctgctttgg caccttctgc
     6241 tgcgaaagca atagccagaa aacttataga agccaaaaat cctgttattc atgcaggagg
     6301 aggagtactc cttgcaggtg cgtctaaaga gcttgaggaa ctggtggatt ttttggatat
     6361 tcctgtttca aggaccctta tgggacaggg ctgcatttca gatacacatc ccttgatgct
     6421 tggacagaca ggattctggg gtcttgagtt tacccacagg cttacggcaa atgcagattt
     6481 gatacttggt ctgggaacac ggtttgcaga ggctgacagc tcaagctggt acccaggtgt
     6541 tacctttgac atgaacaaaa cagatttttt gcagatagat attgatcctg cagaaatagg
     6601 caggaattat cctgtgtcaa tcggtgcaat tgccgactta aagctggctc ttgggcaaat
     6661 tcttgaggaa gtaaaaattc ttttaccgga aggcattgac aggcccgagt taaaggaaat
     6721 gattaaaaca aaccgctctg agtttaaaga atcaaatgtt gaaatttcca atgacggaag
     6781 attccccatg actcctcaga gaattttaaa agatgttaaa gctgttatac cggaagatac
     6841 aattattttc acggacgtag gctggaataa aaatggagtt gcccagcaat ttgatattac
     6901 aatacccggg accatccatc acagctcagg acttgcaacc atgggatttg gcggttctgc
     6961 tcttatagga ggcaagcttg ccgcaccaga taaagttgtt ctgactcttg tgggagacgg
     7021 cggctttgga gtcaacccag gagcattggc aacagctgta gaacaaaaca ttcctgttgt
     7081 ttgggttgtt atgaacaatt ctgcctttgg aaccatagca ggattggaat attcaaatta
     7141 tgagactaaa ttcggtacag tatttacaac tcctgacggg aaaccttatt cgcctaactg
     7201 ggctgaagtt gcaaaggctt atggagttga agcagtaaag atagaatctg ctgatgattt
     7261 taaaccggaa cttgaaaaag caatcaagag caataaaccg tacctattgg atgtgccaat
     7321 ggaaaatata gcagtgccaa caccgggcag ctggaacata aatgatattt ataatcccaa
     7381 gccgggcatt gtaaacggaa aattggtaag gaacgaaaaa ggcgagtata tgccgccgga
     7441 ccattccaca tcccataaat aatactgtta ttataaggag gagaacatgt caagaaaatt
     7501 ttcgcttgct tatctgacaa taccggggac taatcctgtt gaccagataa gaattgccgc
     7561 tgaggcagga tatgattttg tcagtcttag accaatccct atgcaccttc caaatgaacc
     7621 cttgtttcaa tttgatttgg acaggaattt gttcaaggat ataagaaatg cattggagca
     7681 caatgacatt aaacttatgg acattgaact tgcacgggtc agggaagact tgaaggttga
     7741 agattatgaa tctgcatttg cggctggagc cgagcttggt gcaacagatg tattatcaag
     7801 tgtttggacc aaggataagg aatttgcata cagggagttt gcaaaaattt gtgatttggc
     7861 agcaaaatat gatttaagag ttaaccttga atttgtaaca ttttccggga tacctgcttt
     7921 ggacggagct ttggaacttc ttgacaaagt agacagacct aatgcatact taatggtaga
     7981 taccctgcat gcccacaggt ccatggttgg tccaaaagac cttgaaaagg ttgacagaaa
     8041 gaagttcggg tttattcatc tttgcgacgg ccccttggag attccatccc tggaggatcc
     8101 ttccatgatt ggcgttgcca gagaaggaag gctgtatacc ggagaaggtg aaattgacat
     8161 taagggaatg cttctggcta tgcctgataa tcctatatca attgagctgc caaattccaa
     8221 agaaatggca gcaaggggag cagcaggcca cgcagcaaga tgtcttgtaa ccgctaaaga
     8281 gtattttgcg aaaaatcaaa tagaatagca taagctgttc aaggaggaaa acatgaatat
     8341 taacattaat acgagaatga ttgcattgct tggaaaacct ttgtctcagt catatgcggc
     8401 aaggatgcag aatgcagcct acaaagctgc aggaattgat atgctgtatt tctactcaga
     8461 agtggaaaat gaccacttgc ctgatgtggt aaatggaata agatacatga gttttgcagg
     8521 ttttgcagtt acaaaaccca ataaggttga aataatgaaa tatgttgatg aaaaagaccc
     8581 cctttgcgaa aagatgaatg caagcaatac agttgtcata ctgcctgatg gaaggctgaa
     8641 agcctacaat accgacggta taggatttat ccgagcatta aaggaagaaa tgccgaatat
     8701 aaatataaaa gaatcaacat ttttctgtat tggagctgga ggcgcaggaa gggcagtaag
     8761 cagtgtatta gcttattacg gagctaaaaa aatatatatt gccaacagga cattatcgaa
     8821 ggctcaggta ctggttgatg atataaataa gaattttgca cctgttgctg aattggttga
     8881 ccttaatgat gaagctgtgt taaaggacaa aatcaggcag tcggatgtaa ttatgaataa
     8941 taccgggctc ggcatggtta caaagcttga agaaaccccc atacccaagg aatttcttag
     9001 ttccggtcaa ttgtgttttg atgcaaccta taatccggcg aaaactcgtt ttcttattga
     9061 agccgaagaa atgggctgca gcataatgaa tggtttaggg atgtcattat accagggggc
     9121 cgaacagatt gaactttgga gtggaaagga tgccccgatt gaagttatgc ggcaggagct
     9181 tatgaatatc ctttcggaga aaaaataagc ataagtattg attgacttcc aacaaaagga
     9241 ggaaacaaaa tgtctgcaat aaaatggttg gatgagcact ttgaagaagc cattatggtg
     9301 gttcttctgg ctcttatatc atgtattatg atggctcaaa tagttgcccg taatcttttt
     9361 aactcaatga cctggcctga ggaattctgt aggtattgct atatttggac agttttttta
     9421 tccctgggat atacggtaaa aaaaggcaat atgcttaagg taggccttgt aatggactta
     9481 ttgcccacaa aagtaagaag ggcaatagaa attttagtta acctgattat tctggcaata
     9541 tgtgtggttt ttttcaggta tgcaataacc tataccggta taatccataa aaccggacag
     9601 atttcaccgg caatgggtat acccatgtgg atgatgtatt tgtgtactgt gactggattt
     9661 ggactggctt caataaggac agctcagcaa gtagtaagca acataagaaa tttcaatgta
     9721 aaagctgaaa caaccctgga agctacttta aaggaagcca tagaagaagt ggaaactacg
     9781 ggaatcaaga ttgatgacga tttaaaaaat tcagggggtg acgagtaatg gctttgtgga
     9841 tattcatagt ttttatggtt gctttggtat ttgctgtacc catagccatg agcatggtcc
     9901 ttggtgcttt gactccgctt tttctcggcg gtaccggcag ctcaatacag cagcttatag
     9961 ctaacagctt ttcaggatca gattcaaccc ctatactggc agtaccctta tttatcctgg
    10021 gtggagtgct tatggctgaa ggaggcatat caaggagatt attcaatttc tttgcttact
    10081 ttgtaggcgg ttttaccgga ggcctgcctt gtgccgtaat attaacctgt ttgttttatg
    10141 gagcgatttc cggttccggt cctgcgacaa cagcagccgt aggctccatg acaataccgt
    10201 tcttagttga aatgggttat aataaaacct gggcagcagg aatgattgca acatcaggag
    10261 gccttggtgt aattatcccg ccctccatac cctttgttct ctactcgctg gcaacgggtg
    10321 tttccacagg agcacttttc ctgggaggcg tattgccggg tattgcaata ggcttaggca
    10381 tgatggttta tgccgtgtat tattgcagga aaaagggaga ggacagagag aaaatcgaca
    10441 aaaaaatgaa ggagctcagg gacaaaggtt tattcaaact ttttaaagag agcttttggg
    10501 cattgctttc accggttatc gtgcttggag gaatttattc aggctatgta acaccaacag
    10561 aagctgcctg tatttcggtg ttttattctt tgtttgtttc attggttata tacaagtcat
    10621 ttacatttaa ggaaatagtc cccttcctgg caaaagctgt gaaaacttat gcacctcttt
    10681 gctttgttct cgcatttgca atagcatttg gcagagttct ggcattagct aaggcacctg
    10741 tgattattga gggatttatt ttagacaact ttacaacaag ctttactgta ttgactggta
    10801 tagtaattgt atttttactc cttggtatgg ttatggatac cggccctgcc atagttatac
    10861 tggcaccgat attactgccg gcagttcaag ctttgggtgt acatcccgta cattttggcg
    10921 ttattatggt ttgctgctta tcaataggat tggcaacacc gccatttggc ttggacttat
    10981 ttgtggcagg aactttatcc gatacccctc caatgactgt tgcaaaaaaa gctatgcctt
    11041 ttataattgc attcggcata gtattgtttg tcataacata tgtaccatgg ctcagtcttg
    11101 cactcctgta aaggattgaa agaataaatt aaaaaaaaga aaggaaggaa aaaaatgaaa
    11161 aaattattga tagtattgtt gatagcaaca atggtgttgc tgcccgcttg tacccagcag
    11221 gcagcacctg aacctgctcc gccagttgaa gaagagcctg aaccatcaga agaagaacca
    11281 tcaggcattg atgcacttga acctgtagtt ttaaagggtg ctgacaatgc aggagttggg
    11341 gctgcagctc agctctatgg tgaacttgtt gcacagaagg taaaagatat taccggaggt
    11401 aagcttactg tagactattt tccaaacagc cagttaggaa atgaccagga acttcaggga
    11461 caaatgcttg ccggtgatat tgattttgta atatgtcaga ctgcacaaac cgtatctttc
    11521 gtaccggaag tagctatatt tgaccttcct atggtatttg caaagtacga tgcagcaact
    11581 attgattatg cattgaacaa gagcgatttt accgaaaaaa taaaccaggc ctaccaggca
    11641 aagaatatgt actgtttgca cttcctgcag ggaggaacat ttagagaaac aacctctaac
    11701 aaaaatattc agtcaataga tgatttcaac ggattaaaga taagaaccat ggaaaacaaa
    11761 aatcacatgg cattctggca ggcaatggga gcagcaccta caccgcttcc atggccggag
    11821 gtatatattt ccttacagca gggattggtg catgcacagg agaatgctac agatacttgt
    11881 gtaggagcta atctgcaaga agttcaggat tacctgatta tgacccacca tatactgtac
    11941 tgcaatcagt tcttgatcaa tgcagaaaaa tttgacagtc tggacccgtt ataccaggaa
    12001 gcactgcagc aggctattga tgaagcagct gtagaaattg aagctcagct gactcaaatt
    12061 aacgcagaca ataaacagac gcttattgac ggcggcatgg aattagtgga gtttgatgca
    12121 agctttattg accaggtatt ggataaggca aagggtgtat acgaatcagt aggaaatgat
    12181 attggacagg actatgttga ttcgctgctt gaagcacttg aaaaataaat cactcctgat
    12241 ttattttaag ttataaaaat ggtaaataaa accgccggat tatccggcag ttttatttca
    12301 tttaagagag gttattatgg atactgtaag ggcaggtctt gcaggtgccg gttatgcggc
    12361 acatctgcac tgcgaagctt ataaaagagt aagcggtgtt aatgtgaggc tgaaaacaat
    12421 agttgatata gatatagaaa aagctgaaga aataagaaaa aaatataatt ttgagaatgc
    12481 ggttaaaagt tttgacgagc tcctgaaaga tgatgaaatt gaccttatag acattgttac
    12541 tcctccttat ctgcacaaag atatgattat cagggctgtg aatgcgggga aacatgttat
    12601 ctgcgagaag cccttgacag gatatttcgg aaaagcagga gacagtgaac caatagggct
    12661 gatggtttca aaggagaaaa tgtttaatga tgttatacgt gaactggatg aaatcaagga
    12721 tgctgtaaag aaaagcggaa aacatttcat gtatgcagaa aactttgttt atgctccaaa
    12781 tgtacaaaaa acagcagaaa tcatcagaag caaaaaaagt agaattttat ttatgaaggg
    12841 cgaagaaagt cttagaggtt caagctcgcc tttggcaggc aggtgggata aaacaggcgg
    12901 aggatctttt ataagagtag gctgccatcc tttgagcgga attttgtggc ttaagcagga
    12961 agaagcaaaa gcaagaaata ctgaaataaa aataaagagc gtgtctgctg atatgggatg
    13021 tgtaagcaag aatctttccg acctggaaaa aaagcatctg actcttgctc ccgtggatgt
    13081 tgaggatatt gcagctgtaa ccgttacatt ttcagatgaa agcaaggcat tgataattgc
    13141 agcagataat tgtctgggag gaaccagaaa ttatatcgat gtctattgca ataacggctc
    13201 aatgctttgc aatattactc caacagatat tttacagaca tattttctgg atgaagaggg
    13261 tatggatgat gtgtacatat cagaaatgct gccaagcaag ctggggtgga ataaggtatt
    13321 tattgtggat gaagtgctca ggggatacac atcagagctt caggacttca tggagtgcgc
    13381 atcaatggat agagaacctt tgtcatctat tgacctggca tatgatgcca ttaaaacaat
    13441 atatgccgcg tattggtctg cagaagaagg acggagaatt aatttttaaa ttataagaaa
    13501 gaattaataa atatattaaa gagcaatgaa gataattgaa gtattgattt attaattaag
    13561 gcactccacg tcaaaattcg cagggtgcct ttctttatgg atagtataag tttatttcgt
    13621 aatactaaaa ttatttataa taaagtctct tattttaaca gctgcaggtg atgaatattt
    13681 gtctgtccag cataaatata tttcccttac taatggcatt ttgcagattt taaccgtatt
    13741 gatatgcttg tacatagatg gaatttccgg aactatggaa acacctaaat tcagagaaac
    13801 aaatgaaatc acggcattac attcttcagc ttcaaaaata atatttggag taaagtttga
    13861 cctagcaaat atgtcatcaa cggtagacct tagagagctg cttttgctta aggctacaaa
    13921 gggctcatta gctaaatcag aaacagcaat gcattttttg tgagaaaagg ggtggctttt
    13981 cggtatgata agataaaggg gctgctcgaa aatttttacg cttttaaagc cgtcatcagg
    14041 ttttggacaa atagccaggt ctattttatt ggatttcaaa tcttttaaca actgttcact
    14101 taaattttgc accaatgaaa atttgatatt tttgtttgaa tcattttcat agaacttttt
    14161 aatcatttca ggaataaatt ttgaactcat gctgtaaata tatccaagcg atacttcccc
    14221 cgtggaagaa tcggctaggg ttttcagcat cattgtgcct tcgtctaggg tatccaatga
    14281 tttttcaaca tatttaagaa actgctcccc tgcatagctt aattttatac ttctcccgtt
    14341 tctttcaaac aatttgacat ttaattcgtt ttccaactgg gatattgaat agcttaagct
    14401 tggctgagca ataagaagta tttcagctgc ttttgtataa tgttgaacct tagctaatac
    14461 tcgaaaataa tacaactgct gcaatgtcat cttaaacctc ctagttctca atgataatca
    14521 gcttttatag tattataaca gattaattat agaatgtctc tataaatttg gaaatatttt
    14581 tagatatcgg ttgattgctg ttgattatga atacagtctg ccagcccgtg gcattgatat
    14641 attaagtcaa catcagtgta atggtgtacg taactgcgta aactcaaaag tgaaatagca
    14701 ttacaatatc aatacaataa acattgaatc attttagttt ttattatata atgttaataa
    14761 cgtttgcagg aaattccatc gcgtcccctt agtttaaagg tatgtctacg gagtttgcct
    14821 ttgtaatgca gtacatcaat catgaaagca ggtttgtcat ggaaagtgct aagaaatata
    14881 aatatatggg ttttatttta cttcaaagtg taatttacgg cttcggcaat ccgctgacca
    14941 aaattgcctt tgaaagcatc actccctttt ggagtcttgc aatccgtttc acaacagcat
    15001 ttttggtttt tatgttattt ttcggtaagc ggataatcga atctttaaaa agcgttgagc
    15061 ctaaggttta tctgcctgct agcatatgca tggcagcggc ctacattacc tgcaatcttg
    15121 cattgaactg gacttctgca acaaatgtag gatttttaat gagcttgcct gtgatttttg
    15181 cacctgtgct tgcactcttt ttatataagg ttaaatacag gctcatacac attcctatac
    15241 aaattgtggt ggttatcggc ttatatcttc tatgcttcga cggagaaaaa ctggttttcg
    15301 gaaaaggaga ggtaacagca ttaattacag ccctgtcaat agcaggagca ctggtattcg
    15361 gtgaggaatc cctaaagcag ctggatgctg tggcagtatc tgctgcccag gcaggcataa
    15421 ctgctttaat aagcctgccc tgtgcattga tttttgaaag catagaggtt ttaagccggg
    15481 taaccctgcc tgcatggtta gtggttttgt atcttgcgat attttgtacc aatattgcct
    15541 acatgctcca aaatacagcc ctaaaacaca tatcagcagc agctgtgtcg atgcttcaat
    15601 gtacccagcc cattctgacg gcaataattt cttattttat tctaagagaa aagctcagca
    15661 ctttaggagt tataggtgct gtaataataa tcatgtgtat aattgcagaa aatataataa
    15721 ctgctaaaga acaagcccct tccgaaaatc
//
LOCUS       DAOPUE010000036        15744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3802_length_15744_cov_10.352476, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000036 DAOPUE010000000
VERSION     DAOPUE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15744
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3802_length_15744_cov_10.352476"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..4866)
                     /locus_tag="PKH42_04910"
     CDS             complement(88..4866)
                     /locus_tag="PKH42_04910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOA19803.1"
                     /translation="MSQITFIDAEVVPETGKIIDFGAIRPNGEYIHSASLDIIRGFLF
                     ENDFLCGHNIIAHDLKYLNKYLGQIKCVGVIDTLHLSALLFPQRPYHSLLKDDKLLTD
                     ELNNPVNDSKKAMDLFFDEIGAFKLLPEEMKKILYLLLNHKKEFKDFFQYIDYKSEGT
                     NLEAETEIRNFFNGRLCDNADFKHLIANHPIELAYCLSLINADDEYSITPPWVLMTYP
                     YVDSIMNQLRGTKCLFGCEYCNEMMDAVKGLKRFFGYNAYRSFDGVPLQEQAVKSAVN
                     GDSLLAVFPTGGGKSITFQVPALMAGRNTKSLSVVISPLQSLMKDQVDNLEKINITDA
                     VTINGLLDPLERADAIKRVENGDVHILYIAPESLRSKTIEKLLLGRKIARFIIDEAHC
                     FSAWGHDFRVDYMYIADFIKQLCEKKNLDYIIPVSCFTATAKRNVIEDIKKYFREKLN
                     LELKLYTANSGRENLTYKVIPNDRSEKYETLRNLIEYNNSPTIIYVSTTKKAEELAER
                     LTKDGYKAMAYHGRMDKKIKSQNQNEFIRGNVDIMVATSAFGMGVDKKDVGMVVHFDI
                     SDSLENYVQEAGRAGRDQNIKANCYVLFNNEDLDRHFMQLNRTKISIQEIQQIWMAIK
                     NITKMRSKISSSALEIAKKAGWDEGINDLETRVRTAILALENAGYIKRGQNTPRVYAN
                     SILVRNMKDASEKIQASKLFNDNEKQQAIRIMSKLISARSRKRASSEDAESRVDYISD
                     HLGIEKEQVIHIIQLLRQAGILSDNKDLTVFMEESSLGNKSMEKLYEFVELERFLLSH
                     ISNTTVNIKDLNELALDAGVKSSNPDKIKTVFNFWSIKGLVKRYAKGTYYLKIILLKD
                     KDEALKELEERWSIAEFILIYLQKINVNNENALEFSVLEIMGEYNFSMQLFNKKATQS
                     QIEDSLFFLSKIGILKIEGGFLVIYNAISIERLIKDNRIRYKAEDYKTLKDFYKHKME
                     MIHIVGEYAKKMMEDYNSALKFVDDYFRLEYSSFLKKYFKDSKEDELQINMTPERFRQ
                     LFGQLSPAQLRIINDKDSKYILVAAGPGSGKTRILVHKLAAILQLEDVKNEQLLMITF
                     SRAAATEFKKRLSDLMGTKANYVEIKTFHSYCFDLLGLVGNIEKSDDIINNAINAIIN
                     NQVEQSRITKTVLVIDEAQDMDANEYKLVKTLMERNEDMRVIAVGDDDQNIFSFRGSD
                     SKYLSDILNFPNSKMYELVDNYRSTRSIVDFCNYFVRTMKKRLKSMSIAAVSKEKGSV
                     TITEYKSSNLIEPVTNKLIKNGIYGSTCILTMTNEEALQIYALLERNGIKAGMVQRGG
                     IFNLKNLLEIRYFIEEMKLMDGTPLINDEDWENAKKRTFIKYNESDNLPLLRQIISNF
                     EESSGKYKYKSDFSMFIEESREEDFYTDTDKITVSTIHKSKGKEFDHVIMVLRDLNLY
                     QEETKRLIYVGMTRAKKSLSIHFNGEFIERKIGFIDMILRNIKYEFDNEEYPPAEIIS
                     MQLKYNDVYLSYFYKTIKLVQKLNSGDILHFDENGCKDENGVYILLFSSKFKNEIQTY
                     IEKGYKPFSAKVNHILFWKEENKEKETLIVLPQLTFIKSN"
     gene            complement(5045..6391)
                     /gene="glmM"
                     /locus_tag="PKH42_04915"
     CDS             complement(5045..6391)
                     /gene="glmM"
                     /locus_tag="PKH42_04915"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOA19804.1"
                     /translation="MGKLFGTDGVRGIANKDLSPELAYKLGRVGGYFLTNGIKRPKMV
                     VGLDTRISGDMLEGALSSGLNSAGIDVLYLGILPTPAVACMIKILDADGGVMITASHN
                     PIEYNGIKFFDKDGLKLTDETENLIEEYINNNLDIDYIPISGDLGRKIRVEAPLRKYL
                     DYLKSTINVELNGLKIAVDCGNGAVYKAAPQLLHELGAQVYVIHNEPNGININVDCGS
                     TNPEEIQKLVLETGADVGLSFDGDADRLIAADENGNIVDGDHVLAICGTCLKEKGKLR
                     NNTIVGTVMTNLGLDICLKDKGINIIKTSVGDRYVLEEMLKSGYSLGGEQSGHIIFLD
                     FNTTGDGLLTAIQLLSVVKQKNAKLSELSAMMKSMPQVLVNAHVSASKKKDYLTDDVI
                     VNKIKEIEEHFSGKGRVLIRPSGTEPLVRVMIEGENQEEINFHAAELAKLIEERLQ"
     gene            6613..8529
                     /locus_tag="PKH42_04920"
     CDS             6613..8529
                     /locus_tag="PKH42_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOA19805.1"
                     /translation="MIVLSCNNVSKAYMVENIIENISFTVNDNEKVGLIGLNGAGKTT
                     LFNILTENLEPDSGEIYVAKGKKLGYLKQNTIIESNMSIMDEMLLIFDDVIRMEEKLH
                     ELSHKISSISGEKGSAELEALMNTYSELSEQFVEADGYSFRSIIKGVLKGLGFSEDEF
                     DKPINILSGGQKSRVMLAQLLLKKADILLLDEPTNHLDIEAISWLEKYIKNFKGAVII
                     ISHDRYFLDNTVNRIFLMENKYLKSFNGNYTEFVKKRKIELEQAMKQYDEYEREIERQ
                     EEMIRRLHAYGSKRNIRQAFSRQKALDKIKKMDKPSVGSQKFKLKFTPRINSGRDVLS
                     VNNVAVSFDDQQVFKNVNLKIYKGEKVGIIGPNGIGKSTLLKIIAKIIKDYSGEIEYG
                     HYVNIGYYDQEQTNLNNENTVIDEIWDEVPNLNYYTIRKYLAQFLFYGDDIYKLIEDL
                     SGGEKGRLSLLKLMTSNANFLLLDEPTNHLDIDSKEVLEDSLINYEGTILVVSHDRYF
                     LNKVVNKIYDLSEDGAFEYLGNYDYYLEKKAELDETDDIDTITKTKTQINAEKKKERE
                     IIRDRQQREKTIKELEEQIDMHENEIHELEIKLCEPSTYNDNFLIINLNEKLNIRKKE
                     LQELYLLWEELMDN"
     gene            complement(8639..9670)
                     /gene="tsaD"
                     /locus_tag="PKH42_04925"
     CDS             complement(8639..9670)
                     /gene="tsaD"
                     /locus_tag="PKH42_04925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOA19806.1"
                     /translation="MKENINEKTLILSIETSCDETAASVVENGRNVLSNIISSQIETH
                     KEFGGVVPEVASRKHIENINDVVSKAMSEANAEPKDIDAIAVTYGPGLVGALLVGINF
                     AKGLSYAWEKPLIGVNHIEGHINANYIENKELQPPFLCLVVSGGHTHLIHVKDYGVYK
                     ILGMTRDDAAGEAFDKVARAIGLGYPGGPLIDMAAKKGKRDAIKFPRAIIDDETLDFS
                     FSGLKSAVLNYLHNSKQKNETVNTEDIAASFQEAVVDVLVMKTMKAAKQENISTIALA
                     GGVASNSRLRERFKEECLMHNYIFMRPSPILCTDNAAMIGCAGYYKYLNKRFADSSLN
                     AIPNLELAN"
     gene            complement(10081..11382)
                     /locus_tag="PKH42_04930"
     CDS             complement(10081..11382)
                     /locus_tag="PKH42_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19807.1"
                     /translation="MNNENTSERLNNSKVSTWLADNVVSLIFVAFTIFGFLVSDGVTM
                     NFFLTELSSRFFRNAFLVLSLIIPVVAGLGLNFGIVVGAMAGQIALAIVLYFGWGGVN
                     GLLLTMLIALPIATLFGYLTGLLYNKTRGQEMIASLMVGYFANGIYQFLFLFVVGVII
                     KVPNTHPMIKPDGVGVRMTVDMGAYDKGGLKYALDKIWEIPFMWAILAVFACILLLQI
                     WKIVRNKKNPTLYRYNMTMTAINIILSLVMIAISVHAITTNSSLMIVRKVPVITGLLI
                     VALCIFNELILKTKLGQDFRSVGQSQHIAEVSGINVDKTRIIATIMSTVFAAWGQIMY
                     LQNMGTLNTYGAHMQIGLFSVAALLVGGASTQKAGVKHAIFGTLLFNSMFIMSPEIGL
                     SLFGNALLGEYFRTFMVYGVIGLALGLHVWRTNKKGKITLN"
     gene            complement(11382..12482)
                     /locus_tag="PKH42_04935"
     CDS             complement(11382..12482)
                     /locus_tag="PKH42_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19808.1"
                     /translation="MVEVKKKKATFNDIVNKIGFPTIFIGGFWILLLIAGGALGISII
                     TLLSDTIKRAGMNGILVLAMVPSIQSGTGPNFALPVGIVCGLFAIVCSIEFGFTGWAW
                     LFVAIAFAIPLATVVGYLYGRLLNAVKGSEMTIATYTGFSIVAAMCLAWLMIPFQSPN
                     MGWFIGKGLRETIQLDAIGGAHILSNFLRFSLFGENGLITINGETGIIIPTGMLLMFF
                     ICCTIVWLYFRSKSGIAISAGGINPKFAQSSGINIDRCRIKANVLSTILGAIGIIVYS
                     QGYGYAQLYTAPLMMAFAAVAAVLIGGASASRAKVSNVIIGVLIYQGLFTTALPVANQ
                     LFEGTDLSEILRMVIQNGIILYALTKVKGGDN"
     gene            complement(12482..14077)
                     /locus_tag="PKH42_04940"
     CDS             complement(12482..14077)
                     /locus_tag="PKH42_04940"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOA19809.1"
                     /translation="MSDNLLSLKNVSKEYSGNRVLKDINIELKKGQIHALIGENGAGK
                     STLMNILFGMSVIHTTGGFEGTIEIEGKQVDIKSPHDAMDYGIGMVHQEFMLIPGFTI
                     MENIKLNREITKPNLVSPIFGKRLETLNIKRMNEDTRKALDVLDMDIEEYVQVAGLPV
                     GHMQFIEIAREIDKTGLKILVFDEPTAVLAETEAANLLKAMKKLAQKGIGILFISHRL
                     DEIIEVADVVTILRDGEHVVTKNTEDTNKYEIAQLMVGRSITIEREESTNRNIVDETI
                     LDIKNLHVAMPGERVKGVDLKVKKGEIVGIGGLAGQGKLGIANGIMGIFPAEGEVEFK
                     GKKSALNNPKSAIKSGIAFVSEDRKGVGLLLDESIESNIAFSAMQINDEFLVNYGPIK
                     LKDSAKIREHANKMIKDLDIRCTSSLQRVGSLSGGNQQKVCVARALTMKPELLLVSEP
                     TRGIDIGAKKLVLDLLKQLNNEGMTIIMTSSELAELRSICDRILIICEGKVEGVLPPD
                     APDAEFGLMMSGSRNGKSEGSEA"
     gene            complement(14197..15420)
                     /locus_tag="PKH42_04945"
     CDS             complement(14197..15420)
                     /locus_tag="PKH42_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOA19810.1"
                     /translation="MKRLLSLLLALMMVLSLAACTQPAQTPPAEEKPAEEKPAEEKPA
                     EEKPAEEEPFEGIIAIVTNTLSQNEEEYRSAELMVQTYGEDKIMHVLWPDNFMTEQEQ
                     MISTIAKIGSNPDVKGLIINQAVPGTNAAVDKLLETRDDIFISYCTPQENPPDVAERA
                     DLILNPDELKMGDAIPIQAQKLGAKTLVHYSFPRHMSIVMLAARRQLMIDKCKEIGLE
                     FYDATAPDPTSDVGVPGAQQFILEDVPKLVDRFGKDTAFFSTNCAMQTPLIKATSDAG
                     AIYPQPCCPSPYHGYPSALGIESTGYSVDAMANVISETAKALKAKGVLGRFSTWPVPV
                     AMMNTVASTEYIIEWINGNVGDELDVEVLEEKMAEYAKLAVTTSSYTEEGLEIPHFRL
                     IMMDFLTYGEEHILD"
BASE COUNT         4711 a         3074 c         2515 g         5444 t
ORIGIN      
        1 ccaaaggagc attccattta tattccctac atggaaattc ctttgggaga ataacacaga
       61 ctgttcttct ctgaaattcc tctgtaatca gtttgatttg ataaatgtta gttgagggag
      121 aactatcaat gtttcctttt ccttgttctc ttccttccag aataatatgt gattaacttt
      181 agcagaaaaa ggtttgtagc ctttctctat ataagtttga atttcgtttt taaactttga
      241 tgaaaaaagt aatatatata caccattttc gtccttacag ccgttttcgt caaaatgtag
      301 tatatcacca ctattcagct tttgaactaa ttttatggtt ttgtaaaaat acgacagata
      361 cacatcgtta tactttaatt gcatggaaat aatttctgca ggcggatatt cttcattatc
      421 gaattcatat ttaatgtttc tcaaaatcat atcaatgaat ccgatttttc tttcaataaa
      481 ttcaccgttg aaatggatgg ataaggactt tttagctctt gtcattccga cataaatcag
      541 tctctttgtt tcttcctgat ataaattcaa atctcttaat accattatga catggtcaaa
      601 ttcttttccc ttggatttat gaattgtaga gactgttatt ttatcagtat cggtataaaa
      661 atcttcttct cgagattctt ctataaacat ggaaaaatca gacttatatt tatattttcc
      721 ggaactttct tcgaaattgg atattatttg tcttaataaa ggcagattat cactttcatt
      781 atactttata aatgttcttt ttttcgcatt ttcccagtct tcatcattaa ttaatggagt
      841 tccatccatt aatttcattt cctctatgaa gtatcttatc tcaagaaggt ttttcagatt
      901 aaatattcct cctctttgaa ccattccagc ctttattccg ttcctttcaa gcagtgcata
      961 tatttgaaga gcttcttcgt tagtcatagt aagaatgcag gtagaaccgt agataccatt
     1021 tttgattaat ttatttgtta caggctcgat taaattactg cttttgtact ctgttatggt
     1081 aacactacct ttttcttttg atactgctgc tatagacatg ctttttagac gttttttcat
     1141 tgttcgtaca aaataattgc aaaaatcaac aatacttctt gtacttctgt aattatccac
     1201 caattcatac attttagagt ttggaaaatt taaaatatcg ctcaaatatt ttgagtctga
     1261 gcccctgaat gaaaaaatat tttggtcatc atctccgaca gctattaccc tcatatcctc
     1321 atttctttcc ataagggttt tcacaagttt gtattcatta gcgtccatat cctgtgcttc
     1381 atcaattacc aataccgtct ttgtgattct tgactgctcc acttgattgt ttattatagc
     1441 atttattgcg ttgttgatta tatcgtctga tttttctata tttcctacca accctaacaa
     1501 atcaaagcaa taggaatgaa atgtttttat ttctacgtag tttgcctttg ttcccatgag
     1561 atcagataat ctttttttaa attctgtagc tgcagccctt gaaaaagtaa tcattaaaag
     1621 ctgctcattt ttcacatctt caagctgtaa tatagctgct aatttgtgta ctaaaatcct
     1681 tgttttgccg cttcccggac ctgctgccac taaaatatat ttagaatctt tatcatttat
     1741 tattcttaat tgagcaggtg aaagctgacc gaaaagctgt ctgaatcttt caggtgtcat
     1801 gtttatttgg agctcatctt cttttgaatc tttaaaatat tttttcaaaa atgagctgta
     1861 ttccaatcga aaataatcat ccacgaattt aagtgctgaa ttgtagtctt ccatcatctt
     1921 ttttgcatat tcgcccacta tatgtatcat ttccatctta tgtttgtaaa aatctttaag
     1981 agtcttatag tcctcagcct tgtatctaat cctgttatcc ttgattaatc tctctatact
     2041 gattgcattg tatataacaa gaaagccccc ttcgattttt agaattccta tttttgataa
     2101 gaagaaaagt gaatcctcta tctgcgattg ggtggctttt ttattaaaaa gctgcatgct
     2161 aaaattatat tctcccataa tttcaaggac agaaaattcc aaggcatttt cattatttac
     2221 atttattttt tgaaggtata ttaggataaa ttctgcaata gaccaccttt cttctaactc
     2281 cttcaaggcc tcatctttat cttttaaaag aatgatctta agataataag taccttttgc
     2341 atatcgcttt acaagaccct ttatcgacca aaaattaaag actgttttta tcttatcagg
     2401 attagaactt tttacaccgg cgtccaaagc aagctcattt aaatctttta tatttacggt
     2461 tgtgtttgaa atatgcgaaa gcaaaaaacg ctccaattca acaaattcgt aaagtttttc
     2521 catggatttg tttcctaaac tgctctcttc cataaaaaca gtcaaatctt tattatcgga
     2581 taatatgccc gcttgtctta atagttgaat aatatgtata acctgttcct tctcaattcc
     2641 caaatgatct gaaatgtaat caaccctgga ttctgcatct tcactgcttg ccctttttcg
     2701 gcttcttgct gatataagtt tgctcattat tcttatagcc tgctgttttt cattatcatt
     2761 aaacaacttg gaagcttgaa ttttttctga tgcatccttc atgtttctta ccaatatact
     2821 gtttgcatat actctgggtg tgttttgccc tcgcttaata taacctgcgt tttccaaagc
     2881 taaaattgcc gttctgactc ttgtttcaag gtcattaata ccttcatccc agccggcttt
     2941 cttggcaatt tccagtgctg aacttgatat ctttgatctc atcttggtta tattctttat
     3001 tgccatccag atttgctgta tttcttgtat gctgattttt gttcgattca gttgcatgaa
     3061 atgcctgtct aaatcctcat tattaaataa aacataacaa ttggctttta tgttttggtc
     3121 tctgccggct cgtccagctt cctgaacgta attttccagt gaatcggaaa tatcgaaatg
     3181 tacaaccatt cccacatctt ttttatctac acccatacca aatgctgaag ttgcaaccat
     3241 aatgtcaaca ttaccacgaa tgaattcatt ctggttttga ctttttattt ttttatccat
     3301 tcgcccatgg taagccatgg ctttatatcc gtctttggta agtctttctg caagttcttc
     3361 ggctttttta gtggttgaaa catagatgat ggtaggagaa ttgttgtatt caataaggtt
     3421 tctcagtgtt tcgtatttct cggatctatc attaggtatt actttgtagg tgaggttttc
     3481 cctccctgaa ttcgcagtat aaagctttaa ttccaaattg agtttttctc tgaaatattt
     3541 cttaatatct tctataacat ttcgttttgc tgtagctgta aagcatgaaa ccggtattat
     3601 gtagtcaagg tttttctttt cacacagctg tttaataaaa tcagcaatat acatataatc
     3661 aactctaaaa tcatgccccc aggcagaaaa acaatgggct tcatcaatta tgaatcttgc
     3721 aatcttacgg ccaagtaaaa gtttttcaat ggttttcgac ctaagagatt caggcgcaat
     3781 atataatata tgaacatcgc cgttttcaac tcttttaatg gcatctgctc tttctaatgg
     3841 gtctaaaagc ccgttgatag ttacagcatc agtaatattg attttctcta aattatcaac
     3901 ctggtctttc ataagagatt gtagtggtga aattacaaca ctaagagatt ttgtattcct
     3961 tccagccata agtgcaggaa cttggaatgt aatagatttg cctccgcccg tggggaaaac
     4021 tgctaaaaga gaatctccgt ttactgcgct ttttactgct tgttcctgca agggaacacc
     4081 atcgaaactt ctgtatgcgt tataaccaaa aaaccttttt aagcctttta ctgcatccat
     4141 catttcattg caatactcac aaccaaaaag acactttgtg ccccgaagtt gattcatgat
     4201 actatctaca tatggatatg tcataagtac ccaaggaggc gttatggaat actcatcatc
     4261 tgcatttata agagaaaggc aataagcaag ctcaatcgga tgattagcta taaggtgttt
     4321 aaaatctgca ttatcgcata atctgccatt gaaaaaattc cttatctctg tttcagcttc
     4381 taaatttgta ccttcacttt tgtaatcgat atattgaaag aaatctttaa actccttttt
     4441 atgattcaat aataaatata atatcttttt catttcctct ggcagtaatt taaaagctcc
     4501 tatttcatcg aagaataaat ccattgcctt cttagaatca tttacaggat tgtttagctc
     4561 gtctgttaac agcttatcgt cctttaacaa tgaatgatag ggtctttgag gaaataaaag
     4621 agcagataaa tgcaaggtat ctataacacc gacacattta atctgcccta aatatttatt
     4681 taaatatttt aaatcatgtg caataatatt atgtccgcat aaaaagtcgt tttcgaaaag
     4741 aaatcctctg atgatatcaa gagaagcgct atggatgtat tctccgtttg gtcttatagc
     4801 accaaagtca atgatttttc ctgtttccgg cactacttcc gcatcaataa atgttatttg
     4861 actcataata cccgtatcct tagtcgaatt atcttatttt agctaaatta tatagcatat
     4921 tggaaaaaat ttcaatgaaa acaagaaaat tcatctggta acatagagac ggaccttata
     4981 tagattgaga aatactctga tagcaaaaag aaccgtccaa ggaaacgtct gaaacagaaa
     5041 ctttttattg aagtcgctct tctatcagtt ttgctaattc agcggcatgg aagtttattt
     5101 cttcctggtt ttctccttca atcataaccc ttacaagggg ctctgttccc gatggtctta
     5161 ttagaaccct tcccttaccg ctaaaatgtt cttcgatttc tttaatttta tttacgatta
     5221 catcatcagt taaataatct ttcttttttg atgctgaaac atgagcattc accaaaacct
     5281 gaggcatact tttcatcatc gcggaaagct ctgaaagctt tgcatttttt tgttttacta
     5341 cacttaacag ctgaatggct gtcaaaagtc cgtcgcctgt ggtgttgaag tccaaaaata
     5401 ttatgtgtcc tgactgctct cctcccaatg aataaccgct ctttaacatt tcctccaaaa
     5461 catatctgtc gcctacgctg gtctttataa tattaatacc cttatcctta aggcatatat
     5521 caagacctaa gtttgtcata actgttccga ctatggtgtt gtttctgagc tttccttttt
     5581 ctttcaagca agtgccgcat attgccaaaa catggtcgcc gtcaacaata tttccgtttt
     5641 catccgctgc aatcagtcta tcggcatccc cgtcgaaaga tagtcccacg tcagctcctg
     5701 tttctaaaac aagcttttgg atttcttccg ggtttgtaga gccgcagtct acattaatgt
     5761 ttataccgtt aggctcattg tgaataacat aaacttgtgc tccaagctcg tgaagaagct
     5821 gaggtgcggc tttataaaca gcgccgtttc cgcaatcgac tgcaatttta agcccattta
     5881 gctccacatt aattgtactc tttaagtaat ccaaatattt tcttaatggg gcttcaacac
     5941 gtatttttct tcctaagtct ccggaaatcg gtatgtagtc tatatccaaa ttattattta
     6001 tatattcttc aattaagttt tctgtttcat ccgttaattt aagtccatcc ttgtcaaaaa
     6061 actttattcc gttgtattca ataggattgt gagatgcagt tatcatcact cccccgtcag
     6121 catcgagtat tttaatcatg catgcgaccg caggtgtggg cagtattccc aaatataaaa
     6181 catctatccc ggctgaattt aatcctgaag acagtgcacc ttccaacata tctccggata
     6241 ttctcgtatc caagccaaca accatctttg gtctttttat tccattagtt aaaaaatatc
     6301 ctcctactcg ccctaattta tatgctaatt caggggacaa atctttattt gcaatacctc
     6361 ttactccatc tgtgccaaat agtttaccca ttcttcctcc aaacaaaaaa atttaatcaa
     6421 tatccgccgt ctaaaaagag gggttatctt tttcgttata ttgtatcata ttttatactc
     6481 taaaaaaaga taccaaaata ttaatttttt atgatataat atcggtgtga agcaagccga
     6541 ttttgcggct tgtacttatg atataatgaa aactaatcat tcataaactt gagcacttga
     6601 aaggatacgg ttatgattgt tctatcatgc aacaatgttt caaaagctta tatggtggaa
     6661 aatataattg aaaatatttc ttttaccgtt aatgataatg aaaaggttgg attaatcggt
     6721 cttaacggag caggtaaaac aaccttgttt aatatattaa cagaaaactt ggagcccgac
     6781 tcgggagaaa tatatgtggc aaagggtaag aaacttggct atctgaagca aaacacaatt
     6841 atagaaagca atatgagcat tatggatgaa atgcttttaa tttttgatga tgttataagg
     6901 atggaagaaa agctccatga gttatcgcat aaaattagca gtatttcggg agaaaaaggc
     6961 tctgcggaac ttgaagcact aatgaacaca tattcagaat tgagcgagca gtttgttgaa
     7021 gctgacggtt acagttttag aagcataatt aaaggagttt taaaaggtct tggattttca
     7081 gaagatgaat ttgataaacc tataaacatt ttaagcggcg gtcaaaaaag cagagttatg
     7141 cttgcacagc ttttgctcaa aaaagcagat attcttttgc tggatgaacc taccaatcat
     7201 ttagatattg aagcaatatc ttggcttgaa aaatatataa agaattttaa aggtgcggta
     7261 ataattattt ctcacgacag atacttcctt gacaacacgg taaacagaat tttccttatg
     7321 gaaaacaaat acttaaaatc gtttaacgga aattatacgg aatttgtgaa aaaaagaaaa
     7381 attgagcttg aacaagcaat gaaacaatat gatgaatatg aaagggaaat tgaaagacag
     7441 gaagaaatga ttcggcgcct ccatgcatac ggaagtaagc gaaatatcag acaggcattt
     7501 agcaggcaaa aggctttgga taaaatcaaa aaaatggata agccgtctgt tggaagccaa
     7561 aaattcaaat taaaatttac accgagaatc aatagcggca gggatgtgtt aagtgttaat
     7621 aatgtagctg tatcttttga cgatcaacaa gttttcaaaa atgttaattt gaaaatatat
     7681 aagggagaaa aagttggaat tataggtcct aacggtatag gcaaatcaac tctcttaaaa
     7741 attattgcca aaataataaa ggattacagc ggggagatag aatatggtca ttatgtcaat
     7801 atcggatatt acgaccagga gcaaacaaac ttaaataatg agaatactgt aatcgatgaa
     7861 atatgggatg aagtccctaa cttaaattat tatacaatcc gaaaatatct tgcacaattt
     7921 ttgttttacg gagatgatat ttataagctc atcgaagatt tgagcggcgg tgaaaaagga
     7981 aggctgtcac ttttaaaact tatgacttct aatgcaaatt ttttgttgtt ggatgaacct
     8041 acaaatcatt tggatattga ttcgaaggaa gtattagaag attcacttat taactatgaa
     8101 ggaaccatat tggttgtttc tcatgacaga tatttcttaa acaaggttgt aaataagata
     8161 tacgatttat cggaagacgg agcatttgaa taccttggca actatgacta ctatctggaa
     8221 aaaaaagcag agcttgatga aacagatgat attgatacta ttacaaagac taagacacaa
     8281 ataaatgcgg aaaagaaaaa ggaaagggaa attatcaggg acaggcaaca gcgggaaaaa
     8341 accatcaaag aacttgaaga gcaaattgat atgcatgaaa atgaaattca tgaattagaa
     8401 atcaaattat gcgaaccttc gacatataat gacaattttt taattataaa tctgaatgaa
     8461 aaattaaaca ttcgaaaaaa agagttgcaa gagctttacc ttctatggga agaattaatg
     8521 gataactaaa aaaagttatc caccatatgt ttgtagtatg tgcataactt tttactgtat
     8581 tacttgtatc aacaacttta tccacagttg ttgatacctt gtttttatag gctgttaatt
     8641 aatttgcaag ttctaaattt ggaattgcat ttaaggatga atctgcaaat cttttgttta
     8701 aatatttata atatccggcg cagccaatca tggctgcatt atctgtgcat aaaatcggag
     8761 aaggtctcat aaatatatag ttatgcatta aacattcttc cttaaatctt tcccttaaac
     8821 ggctgtttga cgcaactccg ccggctaatg caatagtgct tatattttcc tgttttgcag
     8881 ccttcattgt tttcataacc aacacgtcaa ccactgcctc ctgaaaactt gctgcaatat
     8941 cttcggtatt gacagtttca ttcttttgct ttgaattatg taaataattc aaaaccgctg
     9001 attttaaccc gctgaaacta aaatccagtg tttcatcatc aattatcgct ctgggaaact
     9061 tgattgcatc cctctttcct tttttggctg ccatgtcaat caaaggtcct cccggatatc
     9121 ccaatccgat tgctcttgcc accttgtcaa aagcttctcc ggcagcatca tctctggtca
     9181 ttcctaaaat tttatataca ccgtaatcct taacatgaat caggtgagta tgaccgcccg
     9241 agaccacaag acagagaaat ggcggttgaa gctctttatt ttcaatatag tttgcattaa
     9301 tatgtccttc gatatgattt acgccaatca agggtttttc ccatgcatag gaaagtcctt
     9361 ttgcaaaatt aatacccacc aacaatgctc ccactaaacc gggaccgtat gttacagcaa
     9421 ttgcatctat gtcctttggc tctgcattgg cttcactcat ggcctttgat actacatcat
     9481 ttatattttc aatatgtttc cttgaagcaa cctcaggtac aacacctcca aactccttat
     9541 gagtttctat ttgagatgat attatattgg ataaaacatt tctcccgttt tcaacaacag
     9601 aagcagcagt ttcatcacag gatgtttcta tggaaagtat taaagttttt tcgtttatat
     9661 tttccttcat gcgcaatatt ttcctccgta ttttcctgaa ctgattatac tactgtgagt
     9721 ttcataattc aagaagaaaa aatgtaacag tacccccgtc cctttgtcag aattcaagaa
     9781 gaaaaaaaga gatccttcgc tgcgctcagg atgacaaaga gtcataccga acaatcagga
     9841 agaatctcat actaacgtgt ccccatacct ctaataaatg gtaaataata aagatacaaa
     9901 gcgaacattt gcaggacacg actataattc ttagcgaatg caggttaaaa atccgtttaa
     9961 cagcttgcat tgtttgaacg aagtgagttt gcaagctgta ggatttttag ccgtaatgag
    10021 ctttagaatt attcgtgtcc gaaaccgtga gcgtgtattt ttattatttt tatattttaa
    10081 ttagtttagg gtgattttgc cttttttgtt tgttctccaa acgtggagtc ccaatgcaag
    10141 acctataacc ccgtatacca taaaggttct gaagtattct cccaaaaggg cattgccaaa
    10201 cagtgacaat cctatttcag gagacattat aaacattgag ttaaacaata gtgtaccaaa
    10261 aattgcatgt ttcactcctg ccttttgagt tgaagctcct ccaaccaaaa gtgccgctac
    10321 agaaaacaat cctatttgca tgtgggcacc gtatgtgttt agggtaccca tattctgcag
    10381 gtacattatt tgtccccatg cagcaaatac agttgacatt attgttgcaa taattctggt
    10441 tttatcaaca ttaatgcctg atacctcagc aatgtgctgg ctttggccga cacttctgaa
    10501 atcctgtccc agttttgttt tcaaaatcaa ttcattgaat atacataaag ctactatcaa
    10561 taatcccgtt attaccggaa cctttctaac aatcattaaa cttgagtttg ttgtaattgc
    10621 atgaacgctt attgcaatca taaccaagct cagaataatg tttattgctg tcatggtcat
    10681 gttatatctg tacaaggtgg gattcttttt gtttcttacg attttccata tctgcaggag
    10741 aagaatacat gcaaatactg ccaatatagc ccacatgaac ggaatttccc aaattttgtc
    10801 caatgcgtat ttaagtccgc ccttgtcata tgcccccatg tctactgtca ttctcacacc
    10861 tacgccgtca ggtttaatca tgggatgagt attgggaact tttattatta cacctacaac
    10921 aaacaagaat aaaaattgat atataccgtt tgcgaaataa cctaccatca ggctggcaat
    10981 catctcctgt cctcttgttt tgttatagag caaacctgtt aaatatccga acaaggttgc
    11041 tattgggagt gcaataagca ttgttaacaa aagtccgtta acaccgcccc atccaaaata
    11101 taatacgatt gcaagtgcta tttgtcctgc cattgcaccg acaactatac caaagtttag
    11161 cccgagacct gccactaccg gtattatcaa agataaaaca agaaatgcat tcctgaagaa
    11221 acggcttgat aattccgtca ggaaaaagtt cattgttaca ccgtctgata ctaaaaatcc
    11281 aaatattgtg aaggcaacga atattaatga aacaacatta tctgcaagcc atgttgatac
    11341 tttagaatta tttaatcttt cggaagtatt ttcattattc attaattatc gcctccttta
    11401 acctttgtca gcgcataaag aattattccg ttttggataa ccatacgaag tatttcggat
    11461 aaatccgttc cttcaaacag ttggtttgcc acgggaagcg cggtggtaaa aagaccctgg
    11521 tatattaata cgcctattat tacgttagat acctttgctc ttgaagcaga agccccgcct
    11581 attaatacgg cagcaaccgc ggcaaatgcc atcataagcg gtgccgtata gagctgtgca
    11641 tagccgtaac cctgggaata aacaataata ccgatggctc ccaaaattgt agataaaaca
    11701 tttgctttta ttctgcatct gtcaatattt atacctgaag attgggcaaa ttttggattt
    11761 attccacctg ccgatatggc aattccggat tttgacctaa aatacagcca tactatggta
    11821 caacatatga aaaacattaa aagcattccc gttgggatta ttattccggt ttcaccgttt
    11881 atggtgataa gcccattttc tccaaataat gagaacctta agaaattact taatatatgt
    11941 gctccaccga ttgcatctaa ctgtattgtt tctcttaaac cctttcctat aaaccatccc
    12001 atattggggc tttgaaacgg tatcatgagc catgcaaggc acattgctgc aacaatcgaa
    12061 aatcctgtgt aagtggcgat tgtcatttct gaacctttaa cagcatttag aagtcttccg
    12121 tacaagtatc ctacaactgt cgccaatggt attgcaaaag caattgctac aaataaccat
    12181 gcccaacccg taaatccaaa ttctatggag cagactatgg caaataagcc gcatacaata
    12241 cctaccggca atgcaaagtt tggacctgtt cctgattgta ttgaaggaac cattgccaat
    12301 accagtatac cgttcatacc cgcacgtttt atcgtgtcgc ttaataaagt aataatggat
    12361 attcccaaag ctcctccggc aataagcaag agtatccaaa aaccgcctat aaaaattgta
    12421 ggaaatccta ttttattaac aatatcattg aaagtggctt ttttcttttt tacttctacc
    12481 attatgcttc actcccttct gattttccat ttcggctgcc tgacatcatc aaaccaaatt
    12541 cagcatccgg tgcatcggga ggaagtactc cttcaacttt cccctcacat attattaaaa
    12601 ttctgtcaca tatgcttctc aactcggcta attcggagga agtcattatt attgtcatgc
    12661 cttcattatt taattgcttg agtaagtcaa gcactaattt tttagcccct atgtcgattc
    12721 ctctcgtagg ctcggaaact aaaagcagct ccggtttcat tgtcaatgcc cttgcaacac
    12781 aaaccttttg ctggttcccg ccgcttaggc ttcctaccct ctgcaaggag gaggtgcatc
    12841 taatatccaa atcttttatc atcttattgg catgctctct tattttagca gaatctttga
    12901 gtttaatggg accataattt actaaaaatt catcgtttat ctgcatggcg gaaaatgcaa
    12961 tattggattc tattgattca tccaataaaa ggccgacgcc tttcctgtct tcgcttacaa
    13021 aagcaatgcc ggatttaatt gcagatttgg ggttattcag agctgatttt ttgcccttga
    13081 attcaacctc tccttcagct ggaaatattc ccattatgcc gttggcaata cctaatttcc
    13141 cttgacctgc aagtccgcct attcctacta tttcaccttt ttttactttt aagtcaacgc
    13201 cttttactct ttcaccgggc attgcaacat gtaaattttt tatatctaat attgtttcat
    13261 caacaatatt tctgttcgta gattcttctc tttcaatagt gatacttctt ccgaccatca
    13321 actgtgctat ttcatatttg tttgtatctt ctgtattttt tgtaactaca tgctctccgt
    13381 ctcgcagaat ggttactaca tccgctactt caataatttc atcaagtctg tggcttataa
    13441 ataatatgcc gattcccttc tgggcaagct ttttcattgc tttcaaaaga tttgcagctt
    13501 ctgtttccgc taataccgca gtgggctcat caaaaacaag aatttttaat cctgttttat
    13561 caatttctct tgcaatttca ataaattgca tatgaccaac aggaaggcct gctacttgta
    13621 catattcttc tatatccatg tccaacacat ccaaggcttt ccttgtgtct tcgttcattc
    13681 tttttatatt taatgtttcc aggcgttttc cgaatatggg gcttactaag ttgggttttg
    13741 taatttccct gttcaactta atattttcca taatagtaaa cccggggata agcataaatt
    13801 cctgatgtac catgccgatt ccatagtcca tggcatcatg aggacttttg atatctacct
    13861 gtttgccttc tatttctatg gttccttcaa atccgccggt tgtatgaatt accgacattc
    13921 caaatagaat attcatcaat gtggattttc ctgcgccgtt ttcgcctata agcgcatgaa
    13981 tttgaccttt ttttaattca atatttatat ccttcaatac tctatttcct gaatattctt
    14041 ttgaaacatt ttttaagctt agaaggttat cactcattac tacctcctgt ttattgacat
    14101 aactaattag caaaggaatg aatcagaagt tcctaataag aaattctgaa acattcctta
    14161 atttttatat tatttaatta tagtactata tatcgattaa tcaagaatat gttcttctcc
    14221 gtaagttaag aaatccatca tgattaatct gaaatgagga atttctaatc cttcttctgt
    14281 atatgaactt gtagtaacag ctaattttgc atattcagcc attttttctt caagtacttc
    14341 tacatcaagc tcatcgccta cattaccgtt aatccattca atgatatatt ctgtagaagc
    14401 taccgtattc atcatagcaa caggtaccgg ccatgttgag aatcttccta atactccttt
    14461 tgccttcaat gctttggctg tttcgcttat tacgtttgcc atagcgtcaa cggaataacc
    14521 tgtagattca atacctaatg cagatggata tccgtggtat ggtgacgggc agcaaggttg
    14581 cggataaatt gctcctgcat cgctcgttgc tttaataagc ggagtctgca ttgcacagtt
    14641 tgttgagaag aatgctgtgt cttttccaaa tctatcaact aacttcggta catcttcaag
    14701 aataaactgc tgtgctcccg gtactccaac gtcacttgtc gggtcaggtg ctgttgcatc
    14761 atagaattca agtccgattt ctttacattt atcaatcatt aattgacgtc ttgcagcaag
    14821 cattacgatt gacatatgtc tcgggaatga atagtgtacc aatgttttcg cacccaattt
    14881 ctgtgcttgt ataggaatag catctcccat cttcaattcg tcagggttaa gtattaagtc
    14941 tgctctctca gcaacgtcag gtggattttc ttgtggtgta cagtaagaaa taaatatgtc
    15001 atcacgagtt tccaagagtt tatctactgc tgcgtttgtt cccggaacgg cctgatttat
    15061 aatcaatccc ttaacatccg ggttagagcc tatttttgca attgtagaaa tcatttgttc
    15121 ctgctcggtc atgaaattat caggccataa tacgtgcatg attttgtctt cgccatatgt
    15181 ttgaaccata agttcagctg aacgatattc ttcttcgttt tgagaaagag tgttcgtaac
    15241 aatggctatt atgccttcaa acggttcttc ttctgcaggc ttttcttctg caggtttttc
    15301 ttctgccggt ttttcttctg ccggtttttc ttctgccggc ggagtttgtg caggttgtgt
    15361 acaagctgct aacgataaaa ccatcatcag agccaacaat aaacttaata atcttttcat
    15421 tacttgtccc tcctaattta tacacataaa aatatgtatt catattataa ttgaatatac
    15481 ttctcatgtc aaactatttt tgaaaacgtt atatacttct tacttactaa attatggttt
    15541 tgtgtgtgtt gtgcgtacat ttcaccgcat tttttgtatg tcagcaatat ttataaaatg
    15601 ttaataattg taaactttat tcttgcgcat attacaagcg gagcttttga ttatatctat
    15661 ggaatataat tatgggtttg aatggctaaa agggagcttt tgctccctaa tcttccaagt
    15721 tttctaaaaa ctttctcatt tttc
//
LOCUS       DAOPUE010000037        15346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3924_length_15346_cov_10.193382, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000037 DAOPUE010000000
VERSION     DAOPUE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3924_length_15346_cov_10.193382"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..987)
                     /locus_tag="PKH42_04950"
     CDS             complement(28..987)
                     /locus_tag="PKH42_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOA19811.1"
                     /translation="MLTIEKIKEAQKRIAPFVYKTPLLRLNNLDEYLGCQVYTKMESF
                     QKTNSFKVRGAVNKLMTLSEEELNKGIITVSSGNHGKAVAYAATLLGRKARVVIPDNA
                     PKIKVEGIQSYGAEIIYSIPSERYNVARQLSEENGWSLIPPFDDYEIIAGQGTAGLEI
                     LEQLPDADYIVVPIGGGGLIGGLSAAVKEINPKVKVIGVEPALVPRYSESFKAGHPVS
                     LDPNSKSVADALQTLRPGERNYPIIEKYVDKIVTVHEENILKATKFLLTIGKLLAEIS
                     SSITIGAVLQKAVEFEPEDKVVFLISSGNIGIDQFNKFENIDL"
     gene            complement(1024..1947)
                     /locus_tag="PKH42_04955"
     CDS             complement(1024..1947)
                     /locus_tag="PKH42_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOA19812.1"
                     /translation="MEYIGLVLLLLIVVILFATNIRIVPQAHAYVIERLGAYQATWGV
                     GVHVKVPLIDRVVKKISLKEQVIDFPPQPVITKDNVTMQIDTVIFFQITDPKFYTYGV
                     ERPISAIDYLSATTLRNIIGDLELDETLTSREIINTKMRAVLDDATDPWGIKINRVEV
                     KNILPPKEIQNAMEKQMKAEREKREAILIAEGQKSSAILVAQGNKESMILQAEAEKQS
                     AILRAEAKKEATIREAEGEAEAILSVQKATAEGLKMIKDVELNKEVLTFKSFESFEKA
                     ANGQATKIIIPSEIQGLAGLSKALSETIKES"
     gene            complement(1958..2389)
                     /locus_tag="PKH42_04960"
     CDS             complement(1958..2389)
                     /locus_tag="PKH42_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOA19813.1"
                     /translation="MEYIGYAWLGIVVACVAIEAMTVSLTTIWFAIGAIFAWLIYLTG
                     LGLEVQIVVFLLVSIVCLIFTRPIAMEKLKVGRVKTNAESLIGQYFKVESTIDNINNT
                     GTVNVKGQMWAARSLDDQVIEKDEIVIVKKIVGVKLIVEKK"
     gene            complement(2487..4046)
                     /locus_tag="PKH42_04965"
     CDS             complement(2487..4046)
                     /locus_tag="PKH42_04965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOA19814.1"
                     /translation="MKKIIIIVILCVLFSGCSTFFSDVTEIEAVNLQNGVKIVLPKDS
                     KDADIIIDAVNKKNKTDEDITSLLSYEIILKKENDEEVYKLYFDLDNQTAYLAQDKTV
                     FKIRDNIAKQLFLSDAFSYIYISAMYNSYIEVNGQQINPDIEYNWNSRNIEGDFVNNA
                     GLLTGKYEENEKITVSHSDSIDIKHEKQPDSQIIRIYEQGSLFGTGKNLSELISNIKS
                     DGEYLVECESQWHFKENRESYGSQKLKFSISVDIPAQINIVTKENYPGNILLISIENL
                     NDDETVKLETDAVKTNFDLYAYKDKNYYMMPIDLYTQPGEHNVIAVVNEGAEDEYTLE
                     KTLTVSNRSFKTQYLTVSEEMNETHNDNAAIYEFAQLVKPARTESAKEKLWEGTFIMP
                     VEGELTTDFAEIRYVNNEISSSRHSGLDLAAPKGTPVVAPNNGRVTFAMEGLLSPGNT
                     VVIDHGMGLFTSYYHLDTINVKKGDEVKKGDVIGTVGTTGFSTGPHLHYAVSIYNTYV
                     NPYQTLSGIID"
     gene            complement(4290..5240)
                     /locus_tag="PKH42_04970"
     CDS             complement(4290..5240)
                     /locus_tag="PKH42_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19815.1"
                     /translation="MFIKFLIAAVFAGTPLLFGTVGEIMNEKAGHLNLGVEGMMAMGA
                     CAGFMAGFFSNSIYIALLAAFLAGMLGALIYAVLTITFMANQNVTGLTLSIFGVGISN
                     FAGEFMLVNSASNSLKLPEQITAQISNINIPGISTIPVLGPLLFQYNIFVYLGVVIAV
                     TCGIYYSRTKTGLNVRAIGENPGAADAAGIKVTKLKYINVLIGGGICGIGGAYCSMII
                     NGGVWMNNPVNGQGWIAVALVIFASWKPLRAIFGSFIFGAFNILKYYFPKTIFTIPNA
                     FYDMLPFAITALVLVVTSIRKSKENQQPASCGVNYFREER"
     gene            complement(5242..6324)
                     /locus_tag="PKH42_04975"
     CDS             complement(5242..6324)
                     /locus_tag="PKH42_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19816.1"
                     /translation="MNNRSHTPFIRTVKISERSSNDILKIRIAAFVLALTAGGLFILL
                     LGFNPLKLYGTIISGAFRSKMAFQATVKFMIPLLMSSLGVSIAFKMRFWNIGAEGQII
                     VGAVCASYFALFHDNWPHFLLLIVMFAAGAIGGGLWGLIPTYFKSKYDTNETLFTLML
                     NYIALHIITYLRDGPWKDPGSSGFPKIARFTANAQIDKVLGVHFGWIIALILVVIVFI
                     YLKYTKHGYEISVVGESKSTARYAGMDVKKIILRTMIISGGICGITGMIQAAGSDMTL
                     ASSVAGGVGFTAIIVSWLAYLNPFGILLVSFLFSILDKGSSVMQSTYGLSTYFASVLQ
                     GIILFFILGCEFFIRYKFVLTKKGGK"
     gene            complement(6317..7849)
                     /locus_tag="PKH42_04980"
     CDS             complement(6317..7849)
                     /locus_tag="PKH42_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19817.1"
                     /translation="MLKKNDNYAIELKGITKSFGKVIANEDVNLGIKYGEILALLGEN
                     GSGKTTLMNILSGIYYADEGTIFIDGNEVFITNPHEAIKLGIGMIHQHFKLVEVFSAM
                     DNIVLGTKGRYVKNSALKEKILEMSNKFGLEIEPDKKVYNMSVSEKQTIEIMKVLYRG
                     AKILILDEPTSVLTPQETEKLFVILRRMKEQGNAIIIITHKLHEVLAISDRVAILRKG
                     KVVDTVNTRETNEKQLTELMVGRPISLEIKRPETSRKKTVLKVVDLSVVNEDGTIGLN
                     NINFKLKSGEILGVAGVAGSGQRELCETIAGLMHPKTGAVLYNKENIIGKTPDEIINL
                     GISMSFVPEDRLGMGLVGSMDMIDNMLLKSYKKNKGFIIDKKPSRQLAEELVDKLSIT
                     TPNVETPVRMLSGGNVQKVLLGREIESEPNVLITAYPVRGLDINSSYIIYDLLNEQKK
                     KNVAILFIGEDLDVLLELSDRIMVLCHGELMGLVDAKNTTKEEVGLMMAGRKMEGVDS
                     DE"
     gene            complement(7994..9157)
                     /locus_tag="PKH42_04985"
     CDS             complement(7994..9157)
                     /locus_tag="PKH42_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA19818.1"
                     /translation="MKKFLGIILALTMILSLFTACGSNGQQTKPESEGPEVSGEGIFP
                     AIAKEDIKVGVIHIGNPADGSGYTFAHDQGIIEMQKALGLSDSQIIRKNNVNDTDPTA
                     TDAAIRECIEEGAHIIFGTSWGYMDTMEALAEEYPDVIFSHGSGYKSNDRNFNNYFGR
                     IYQARYLTGIAAGLKTKTNKLGYVAAMGKDNSEVTGGLNAFAMGVHSVNPEAKVYVKV
                     TNSWYSPEEETNAAKALIAEGCDVIAQHCDTPNPQLEAEKAGVFGIGYNSDMSKDAPK
                     ATITSAVWNWGAYYTWAVEKVINGTWLSENYYGGMKEGMIDISPINESIAAEGTVEKI
                     EEAKQSIMDGTFNVFDGVIETNDGSTVGEEGKTLDDGTITGGINWYFKTVVEK"
     regulatory      complement(9225..9326)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(9475..9660)
                     /locus_tag="PKH42_04990"
     CDS             complement(9475..9660)
                     /locus_tag="PKH42_04990"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOA19819.1"
                     /translation="MPIIQVEMLKGRTVDQKREMVKEITEVVSRTANCPKEAVRIVIR
                     EMEFENFGISGKLQCDI"
     gene            complement(9693..10784)
                     /locus_tag="PKH42_04995"
     CDS             complement(9693..10784)
                     /locus_tag="PKH42_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOA19820.1"
                     /translation="MERYPMLEFNLKKLYNNTKIVTKLCEDNGIKVAGIIKGFGGIAE
                     GAEEMVKGGCYQIGSSRIEQLKALKDRGFDMPLLLVRIPMMCEIEDVVKYSDISLVSE
                     KETLNLLNKEAKAQNKIYGVVLMYDLGDLREGVFYKEELIRLAEYVEYESDNLYLEGI
                     GSNLSCYGSVAPTTENLSQLAEAATEIEEKLNRKLNIISGGGTTTLPLLVRGGVPKKI
                     NHLRIGEGINNTQDLPLYWDTNIEGLDPDVFVLKAQIVEINEKPTHPIGQLMVNAFGD
                     LPHYEDRGIRKRAIIALGNQDLGDSSRLVPKDKNMIVLGASSDHTILDIHDCDTEYKL
                     GDIIEFNILYQAMLMTTLSDYVHKKIIKY"
     gene            complement(10794..11246)
                     /gene="tadA"
                     /locus_tag="PKH42_05000"
     CDS             complement(10794..11246)
                     /gene="tadA"
                     /locus_tag="PKH42_05000"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOA19821.1"
                     /translation="MDEVFMKEALKEAYKAYEINEVPIGAVIVRKGEIVGRGFNQKET
                     LKDATLHSEITAIKDACKNLGGWRLPGCTMYVTLEPCPMCAGAIVNSRIERLVIGARD
                     LKTGACGSVLDITNNKKLNHQPEVKFGVLEKECTGILQDFFIKLRKKI"
     gene            11592..12611
                     /locus_tag="PKH42_05005"
     CDS             11592..12611
                     /locus_tag="PKH42_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19822.1"
                     /translation="MKTIKKLWALGLCGILVINMNSMAYADVLDDIIGSSTIEDTEDA
                     ENTEDVYTDVDNEDVTGNDITVTDENIAKYSVLFSDIDNHWAKEWIKNAVGLGFVSGY
                     EDGTFKPDRTITRAEFSTMLNKAMKIEITENINFSDVREKEWFYKEIQKSVAAGFFSG
                     YENNTFRPNNPIKREEVAKVVAGAITTGNIDGEGATLLKDYSTIQEWAKDSVNTVYNK
                     GYILGYPDKTYMPSKALTRAEAVKIIFEIVDNENIEYGFNITNYNETYSSAVVVGNLN
                     VLDSVGNGNVYIKNVVVLGDIVISAKNVKSVELTDVKARRIIVEGENNPVRVVYNDNV
                     IIAKK"
     gene            complement(12657..14402)
                     /locus_tag="PKH42_05010"
     CDS             complement(12657..14402)
                     /locus_tag="PKH42_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19823.1"
                     /translation="MEKKNNFMPLLIYSIKEKKNWVLLSTAIIFLTTLLIPYILRVDE
                     EFFIMFGIVELFVLVLINCLVDNSFFHNESKLTYYSSKPVTLGRQIIINIVTNAVFTV
                     YLLALIVLSVIFQGLDYEVLKVFKMLIPWLSAIILLASLSSILSGNTLMAGAMTIFNF
                     CLPLIILLVIMFIFSILENLVLGFSADVLSDYFVNNIYRIDYLYFVRYAESKSIDLVY
                     LLLLPTILIFISLLINKFIKRRKNENTGFIVFNGFKYFVAVLACLIIPASFSIGFSRY
                     MSIGNRLIISALLAVLSYYIIIAFIEKSFRISKSSVKVFIVSMVAFAVITGGTVGVAS
                     QYENLVPEPEDVQMAYVGNQRWSINEIDRFLRGDYTSENSDFPEWKRNRNIITYEDED
                     IIEIITELHEEVLKDQTFYHESDYYGINNFVIAYWMKDGSKIIRDYTLTATDKLTKEH
                     ETKNELANKLLNSIDYKKQKFYYIYDENYYSGRNLYADFKNISNLNNVIKSVYLNDLR
                     GALTKDIENLFIENDKAFIELSMNYDSRFNKAVMAGEQEYILEIYEKISDIDKNYINE
                     INLNKNFVNTLEMLK"
     gene            complement(14386..15276)
                     /locus_tag="PKH42_05015"
     CDS             complement(14386..15276)
                     /locus_tag="PKH42_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19824.1"
                     /translation="MLTINNLSKKFGDFQVLKNININADKGSIYGLVGSNGCGKTTIL
                     KHIMKVYKQDDGSITFNGEEVVEGSDIIKDFYFVQDTLYFPYQYTLNKLFHYEKLMYN
                     MSEEKFEYLLKYFNIDKNKVLNKMSKGQKKQAAFVLSIAAQPRLLLLDEIVDGLDAVI
                     KRKFWDVLIKEIIDNNMTVVISSHDLKELDNICDRVGIMHEGRILREENLEKMKEDIK
                     RVQFAVEGEFDPDKLKEFNVLQVVKIGSVFIATLTGNIDELKKYLESKYTLLLFDKLS
                     MSLEEIFITELGGVGYGKEK"
BASE COUNT         4608 a         3137 c         2285 g         5316 t
ORIGIN      
        1 gcgcccttag gcgcattttt tattttttta caaatctata ttctcaaatt tattgaactg
       61 gtcaattccg atattgccgc ttgaaattaa aaagacaact ttatcttccg gttcaaattc
      121 aacagccttt tgcaggactg cacctattgt aatagaggaa gaaatctcag ccaataattt
      181 ccctatcgtt aataagaatt tagttgcctt aagaatattt tcttcatgaa ccgtaacaat
      241 tttatcaaca tatttttcta ttatggggta attacgttct cctggtctta aggtttgtaa
      301 ggcatcagcc actgattttg agtttgggtc caaagaaacg ggatgacctg ctttaaagct
      361 ttcactgtaa cgaggaacca atgccggttc aactccgata actttaactt ttggattaat
      421 ttctttgact gctgcagaaa gcccgccgat taaaccgccg ccgccgatgg gaacaacaat
      481 ataatctgca tccggaagct gttcaagtat ttctaatccg gcagtacctt gtccggcaat
      541 aatttcataa tcatcaaacg gaggtattaa gctccagcca ttttcttcac ttagttgtct
      601 tgccacatta tatcgctccg atggtatact gtagattatt tcagctccat atgattgtat
      661 tccttcaact tttatcttgg gagcattatc cggaattact actctggctt ttcttcctaa
      721 caaagtagct gcatatgcta cagctttacc gtgatttccc gacgaaacag taataattcc
      781 tttattgagt tcctcctccg ataaagtcat taatttatta accgcaccgc gaactttaaa
      841 agaattggtc ttttgaaatg attccatttt agtgtatact tgacagccta agtattcatc
      901 caagttattt aaacgaagga gaggagtctt atacacaaat ggtgcaatgc gtttttgtgc
      961 ttctttgatt ttttcaattg taagcatgag cttaaccccc tagactaatt ttaataccat
     1021 taattaagat tcttttattg tttcggacaa tgcttttgaa agtcctgcaa gtccctgaat
     1081 ttccgaaggt attattatct ttgttgcctg accgtttgca gctttttcaa acgattcaaa
     1141 actcttgaat gtaagcactt ccttgttaag ctcaacatcc ttaatcattt ttaaaccctc
     1201 ggcggttgcc ttttgaacgc ttaaaattgc ttctgcttca ccttctgcct ctctgattgt
     1261 tgcttccttc ttagcttctg cccttaaaat tgctgactgt ttttctgcct ctgcctgaag
     1321 tatcatagat tccttgttgc cttgcgctac caatatagca gaactctttt gtccttcagc
     1381 aataagaatt gcctctcttt tttctctctc ggccttcatt tgtttttcca ttgcattttg
     1441 aatttctttt ggaggcaata tatttttaac ctctacacga ttaattttta taccccaagg
     1501 gtcggtagca tcgtccaaga cggcacgcat tttcgtattt attatttctc ttgaagtcaa
     1561 tgtttcatca agctccaaat caccaataat atttcttaag gttgtcgcac ttaaatagtc
     1621 aatagctgat atcggcctct cgaccccgta tgtataaaac ttggggtctg taatttggaa
     1681 aaatattacc gtatctatct gcattgttac attatccttt gtaataaccg gttgaggcgg
     1741 aaagtcaatt acctgctctt taagagatat tttctttacc accctgtcaa tcaagggaac
     1801 ctttacatgt actccaacac cccatgttgc ttgataagct ccaagtcgct ctataacata
     1861 ggcatgtgcc tgaggtacta ttctgatatt tgttgcaaac aatataacta ctatcaataa
     1921 caataaaacc agacctatat attccatata ctcctcctta ttttttctca acaataagct
     1981 taacgcctac tattttttta actattacta tttcgtcctt ttcaattacc tggtcatcaa
     2041 gagaccttgc agcccacatt tgtcctttca cgtttactgt tcccgtatta ttaatattat
     2101 caattgttga ttcaacctta aagtattgcc ctattaatga ttcagcattg gttttcactc
     2161 ttccgacctt taatttctcc attgcaatcg gcctcgtaaa tataagacat actatagaaa
     2221 ccaacaaaaa aaccactatc tgtacttcta atcctaaacc ggtcaaataa atcaaccacg
     2281 cgaaaattgc gccgatagca aaccatattg ttgttaagct aacagtcata gcttctattg
     2341 ctacacaagc aaccactatt cccagccagg cataccctat atattccatt gatacctcct
     2401 attacgggta cattccttaa gttgtgtccc ccttctaata actttacatt aattatatac
     2461 cacaattcaa acctggtaaa cagttttcaa tcaattatcc cgcttaaggt ttgatatgga
     2521 tttacataag tgttataaat actcactgca taatgcaagt ggggcccggt tgaaaatcct
     2581 gttgtaccca cagttccaat aacatccccc tttttaacct catcgccttt ttttacatta
     2641 attgtatcta agtgatagta ggaagtaaaa agccccattc catggtcaat aacaaccgtg
     2701 ttgcctggag aaagaagtcc ttccatagcg aatgtaaccc tcccgttatt tggagctaca
     2761 acaggagttc cttttggagc ggctaagtca agacctgagt gccgcgaaga agaaatttca
     2821 ttattcacat atcttatttc agcaaaatcc gttgtcagtt ctccttcaac aggcattata
     2881 aatgtaccct cccacaactt ttcctttgcc gactcggttc ttgcaggttt tacaagctgt
     2941 gcaaattcat agattgcagc attatcatta tgtgtttcgt tcatttcttc gctaactgta
     3001 agatattgag ttttaaaact tctatttgac acagtcaatg ttttttccaa tgtatattca
     3061 tcttctgccc cttcatttac aactgctata acattatgtt caccgggctg tgtatacagg
     3121 tctattggca tcatataata gtttttatct ttgtatgcat aaagatcaaa gttagtttta
     3181 actgcatctg tttccaattt tactgtttca tcatcattta aattctcaat actaatcaac
     3241 aagatgttgc ccggataatt ctccttggtt actatattaa tttgtgcagg tatgtctacc
     3301 gagatagaaa atttcaactt ctgacttccg tatgactctc tgttttcctt aaagtgccac
     3361 tggctttcac attcaaccag gtattcaccg tcacttttta tattacttat gagctcagac
     3421 aaattttttc ctgtgccgaa caaactgcct tgctcataaa ttcttattat ttggctatca
     3481 ggctgctttt catgtttaat atctattgag tcggagtgtg acactgttat tttttcattc
     3541 tcttcatatt ttcccgttaa tagtcctgca ttgtttacaa aatctccttc tatatttctg
     3601 ctgttccaat tgtattcaat gtcaggattt atttgttgcc cgtttacctc aatatacgaa
     3661 ttatacattg ctgaaatata tatgtatgaa aaagcatcgc ttaaaaacaa ttgttttgca
     3721 atattgtctc taattttaaa cactgtttta tcctgagcaa gatatgcagt ttgattgtct
     3781 aaatcaaagt aaagcttgta aacttcttca tcattttctt tttttagtat aatttcatag
     3841 ctcaataagg atgttatatc ttcatctgtt ttattcttct tatttactgc atctataatt
     3901 atatccgcat ccttgctgtc ttttggtaaa acaatcttaa caccgttttg taaatttact
     3961 gcttcaattt cggtaacatc ggaaaagaaa gtgctgcaac cggaaaataa aacacacaat
     4021 ataacaataa ttattatttt cttcataata cctccatcaa gtagtttttg attttgacaa
     4081 agtattatac attattatat ggaaattgaa ctatgaaaat acaaaataac acaataatag
     4141 tataacatag atatgctgat atgaaataaa aaaatgcgat atatttaaag agctgtcaaa
     4201 tgacagctct aatttcaaca gatgagattc tttgagctaa ggctttcata attacacgta
     4261 gcttattcat agaaataact aaggtcatgt tatctttctt ctctgaaata atttactccg
     4321 cagcttgcgg gctgttggtt ttcctttgat ttcctaattg aagttactac caataccaag
     4381 gctgttatag caaatggaag catatcataa aatgcatttg gaattgtaaa aattgttttt
     4441 ggaaaataat attttaaaat attgaatgct ccaaatataa atgagccgaa aattgctctt
     4501 aagggtttcc atgaagcaaa tataacgaga gcaacggcaa tccatccctg accgtttacc
     4561 ggattgttca tccatacacc gccgttgata atcatggagc agtatgctcc tcctataccg
     4621 catataccgc cgccaatgag tacattaata tactttagct ttgttacctt gattcctgct
     4681 gcatcagctg caccgggatt ttccccgata gctctcacat tgagccctgt ctttgtgcga
     4741 gagtagtata taccgcatgt tacagcaata actacaccca aatatacaaa aatattgtat
     4801 tgaaataata atggtcccaa aacaggaata gtgctgatgc cgggaatatt tatattgctt
     4861 atttgtgcag ttatttgctc aggaagcttt aaagaatttg aagcagaatt tacaagcata
     4921 aattctcccg caaagtttga tatacctaca ccgaaaattg aaagggtaag accggtaaca
     4981 ttttgatttg ccataaatgt aattgttaaa acagcataaa ttaaagcacc cagcatgcct
     5041 gccaaaaaag ctgccaaaag ggcaatataa atactattgc tgaagaatcc tgccatgaat
     5101 ccggcacatg ctcccatagc catcattcct tcaactccaa gatttaaatg tcctgccttt
     5161 tcattcataa tctcgcctac cgtaccaaat aacagaggag ttcccgcaaa taccgcagca
     5221 attaaaaatt taatgaacat gttatttccc tccttttttg gtaagtacaa atttatatct
     5281 tatgaaaaat tcgcatccta atataaaaaa cagaattata ccctgcagca cacttgcaaa
     5341 atatgttgac aatccatagg tagactgcat gacgctgcta cccttatcaa gtatgctgaa
     5401 caaaaatgaa accaaaagta ttccaaaagg atttaaatat gccagccagg atacaattat
     5461 tgcagtaaat ccaactcctc ctgctacaga agatgctaag gtcatgtctg aaccggcagc
     5521 ctgaatcatt cctgtaatac cgcaaattcc gccgcttata atcatggttc gaagtattat
     5581 ttttttaaca tccatacctg catatctggc agtagattta ctttcaccga ctacgcttat
     5641 ttcatatccg tgtttggtat atttaagata tatgaatact atgacaacaa gaatcagagc
     5701 aataatccat ccgaagtgca ctccaaggac cttatcgatt tgagcattag cagtaaatct
     5761 tgctattttc ggaaaacctg acgagccggg gtccttccag ggaccatccc tcaggtaagt
     5821 aattatatgc aatgctatat aattgagcat caatgtaaaa agtgtttcat ttgtatcata
     5881 ttttgattta aagtatgtag gtatcagtcc ccacagacct ccgcctattg ctcccgcagc
     5941 aaacataaca atcaataaca aaaaatgagg ccaattatcg tgaaacagtg caaaatatga
     6001 agcgcagaca gcaccgacta ttatctgtcc ttcggcacct atattccaaa atctcatttt
     6061 aaatgcaata gaaacaccta aggatgacat taataatgga atcataaatt taactgttgc
     6121 ctggaatgcc attttacttc gaaatgcccc ggatataata gttccataca atttaagggg
     6181 attaaagcct aagagaagta tgaataatcc tcctgcagta agggctaata caaaggcagc
     6241 tattcttatt tttaatatat cattgcttga gcgctcggat atttttacgg tccttataaa
     6301 aggtgtatga cttctgttat tcatctgagt caactccttc catctttctt cctgccatca
     6361 taagcccgac ttcttccttt gtagtatttt ttgcatccac aagacccatt aattctccat
     6421 ggcataaaac cataatcctg tcggacagct ccaacaaaac atccaaatct tctcctataa
     6481 ataatattgc aacattcttt ttcttttgct catttaataa atcataaatt atataagaag
     6541 aatttatatc aagtcctctg acgggatatg cagtaatcaa aacattcggt tcagattcaa
     6601 tttctcttcc caaaagaacc ttctgtacat ttcctcctga aagcattctt accggagttt
     6661 caacattcgg agttgtaata ctcagtttat caaccaattc ttcggctaac tgcctggacg
     6721 gttttttgtc aattataaaa cctttgtttt tcttgtatga tttaagcaac atattatcaa
     6781 tcatgtccat agagcctact aaccccatgc ctaacctgtc ctccggtaca aaactcatgc
     6841 ttatgcctaa atttataatt tcatcgggag ttttccctat aatattttcc ttgttatata
     6901 atacggctcc tgtcttagga tgcataagtc ctgcaatagt ttcgcacaat tctctttgcc
     6961 cgcttcctgc aacacctgca acacctagta tttctccgct ttttagctta aaattaatat
     7021 tgtttaaacc tattgtgccg tcctcattta caacagacag gtctaccacc tttaaaactg
     7081 tcttctttct gcttgtttcg ggacgcttga tttcaagact gataggtctt cccaccatta
     7141 attccgtaag ctgtttctca ttagtctccc gagtattgac cgtatcgact acctttcctt
     7201 ttcgaagtat tgcaaccctg tcactgattg ctaaaacttc atggagcttg tgtgtaataa
     7261 taattatcgc attcccctgc tctttcattc tgcgaagtat cacaaagagc ttttcagttt
     7321 cctgaggtgt gagtaccgaa gtgggctcgt ctaaaattaa aatcttcgcc cccctgtaca
     7381 gcactttcat tatttcaatg gtctgcttct cgctcacgga catattgtag acttttttat
     7441 caggctctat ttctaaaccg aatttgttgc tcatttcaag aattttttct tttaaagcac
     7501 tgtttttgac gtatcttccc tttgtgccta aaacaatatt atccattgca ctgaatactt
     7561 ctaccaattt aaaatgctgg tgaatcattc ctatgcctaa ttttatagct tcgtgtggat
     7621 ttgttatgaa aacttcattt ccatcaataa atattgtacc ttcatcagca taataaatgc
     7681 cggataaaat attcatcaat gtggttttcc ccgagccgtt ttctccaagc aaagcaagaa
     7741 tttcaccata tttaattcca aggttaacat cttcgtttgc aattactttt ccaaaggatt
     7801 tagtaatacc cttaagttca attgcataat tatcattctt tttcaacata atcacctttt
     7861 ttgtcatatc ctgagcgtaa acgaaggatc tcagggttta aaaatgagat tcttcgagct
     7921 aaagctctca gaatgacacc tagcttattc atagcaatgt attaaggggt tggcaacaac
     7981 caaccccatt gaattatttt tctactacag ttttaaaata ccaattgatt ccgccggtaa
     8041 tagtaccgtc atccaaggtt ttgccttctt ctccaactgt ggagccgtca ttagtctcta
     8101 ttactccgtc aaaaacattg aaggtgccat ccataataga ttgttttgct tcctcgattt
     8161 tttcaacggt tccttctgca gcaatacttt cattaatagg tgaaatatca atcatgcctt
     8221 ccttcattcc accataataa ttttcactta accaagttcc gttaataact ttttctacag
     8281 cccatgtata ataagctccc caattccata ctgcagatgt aatggtagcc tttggagcat
     8341 ccttagacat atctgaatta tagcctatac caaaaactcc tgccttctct gcttccaatt
     8401 gagggttggg agtatcgcaa tgctgggcta ttacgtcaca gccttcagct atcaatgctt
     8461 ttgcggcatt tgtttcttct tcgggtgaat accagctgtt tgtaaccttt acataaactt
     8521 ttgcttcagg gttaactgaa tgcacaccca ttgcaaatgc atttagtccg cctgttacct
     8581 ctgaattatc cttacccatt gctgccacat atcccaactt attagttttt gtttttaatc
     8641 cggctgcaat tcctgttaaa tatcttgcct gatagattct tccgaaataa ttgttaaagt
     8701 ttctatcatt gcttttatac cctgaaccgt gtgaaaatat tacatcagga tattcttcgg
     8761 ctaaggcttc cattgtatcc atgtaccccc agcttgtacc gaaaatgatg tgtgctcctt
     8821 cttcaataca ttcgcgtata gctgcatcag tagctgtagg gtctgtatca ttaacattgt
     8881 ttttacgaat tatctggctg tcgctaagac caagtgcttt ctgcatttct attattccct
     8941 gatcgtgtgc aaaggtataa ccggagccat cagcaggatt tccgatatgt attactccaa
     9001 ccttaatatc ttccttggca attgcaggaa atattccttc tccggaaact tccggcccct
     9061 ctgattcggg ttttgtttgt tgtccgttgg aaccgcatgc agtaaacaat gaaagtatca
     9121 ttgtcagcgc taaaataata cctaaaaatt ttttcatttt atcctcctat gtaataaact
     9181 ttgcatcaag aaataaaaaa tgcgaaaagt atccctaatc gcatcaatag aataatacta
     9241 tcaatagtca aacaatttaa ggttgtttgg tagaaactat ggaaccatat tatccatatt
     9301 atattgatgc agtctatata ttcaacataa ttataactta agctgtgaca ataatcaaca
     9361 aattattttg ttatcagccc ggtgggacag ggttcgcaga aataatttaa tattaatagt
     9421 tgaatactaa ctaatgctca gatacagaat ttatcagtaa ttagaaggtc agtcttaaat
     9481 gtcacattga agctttccgc ttattccaaa gttttcaaat tccatttctc ttataacgat
     9541 tcttacggct tcctttggac aattggcggt tctcgatact acttccgtta tttccttaac
     9601 catttctcgt ttttggtcaa cggtccttcc ctttagcatt tctacctgaa taatgggcat
     9661 gattccacct cctctttctc aatatggtaa atttaatact ttattatttt tttgtgtaca
     9721 taatcggaca aggtggtcat cagcattgct tgatacaaaa tattaaattc aattatatct
     9781 cctaatttat attctgtgtc gcaatcgtga atatccaaaa tggtatggtc gctgctggct
     9841 cctaatacaa tcatattctt atcctttgga acaagccttg atgagtcccc taaatcctga
     9901 tttcccaatg caataattgc cctttttctg attcccctgt cttcgtaatg aggcaaatct
     9961 ccaaaagcat ttaccattaa ttgtccgatg gggtgagtcg gtttttcatt tatttcaaca
    10021 atttgagcct ttaaaacaaa tacatcaggg tctagacctt caatatttgt atcccagtaa
    10081 agaggcaaat cttgtgtatt atttattcct tctccaatac gaagatgatt tattttcttt
    10141 ggaactcccc ctctcaccaa aagaggcagc gtggttgtcc ctcctccgga tattatgttt
    10201 agttttctgt ttagtttttc ttctatttct gttgctgctt ctgccaattg acttaagttt
    10261 tccgtagttg gagcaactga gccgtaacag cttaaatttg agcctatacc ttcaagatat
    10321 aaattatctg attcatattc aacatattcg gctaatcgaa tcaattcttc tttataaaat
    10381 actccttctc ttaaatcccc caaatcatac ataagcacaa cgccgtagat tttattttga
    10441 gcttttgctt ctttatttag caaattcaga gtttcttttt cagaaaccaa ggatatatca
    10501 ctgtatttaa cgacatcctc aatttcacac atcatgggga ttctgaccaa taacaaaggc
    10561 atatcaaatc ccctgtcctt caaggctttt agctgctcaa ttcgggagct gcctatttga
    10621 taacagcctc cttttaccat ttcttcggca ccttccgcaa ttccgccgaa tcctttaatt
    10681 atgcctgcaa ccttgattcc gttatcttca catagttttg taacaatttt ggtattgttg
    10741 taaagttttt ttaaattgaa ttcaagcata gggtatcttt ccataggagc ctcctatatt
    10801 ttttttctaa gctttataaa aaagtcttgt aaaatgccgg tgcattcttt ctccaatacg
    10861 ccaaacttaa cctccggctg gtgatttagc tttttattgt ttgttatatc caaaacagag
    10921 ccgcatgcac ccgtttttaa atccctggct cctattacca gcctttcgat tcttgaattg
    10981 actattgcgc cggcacacat ggggcaaggc tccaaggtta catacatggt acatccggga
    11041 agacgccatc cgcccaagtt cttacaggca tcctttatgg ctgttatttc agagtggaga
    11101 gtggcatcct ttaatgtttc tttttgattg aaacctctgc cgactatttc tccctttctt
    11161 actattacag caccgatggg aacctcattt atttcatatg ccttgtatgc ttctttcaag
    11221 gcttccttca taaatacttc atccataact cttccttact gcattttatt ttaattatag
    11281 ttttttcatt aattcaagtc aacaatattg tctaagagcc tgtcttaaca taagcagaaa
    11341 ttgcatgtta tttaatatac cttttcaaga aggcttgtct ccttatatta cctgtttact
    11401 ttcttcacaa taaaatatag aattaattat atagttttaa acagcgaaag attccctatt
    11461 agaggtatgg ggacacacct tcaatgaagg gatagccttt ctgggtgagc attgaaggaa
    11521 tgtccccgta aatatccata cttggaacaa atgttaattt gattaagtct aatatatgag
    11581 gaggaggtac tatgaaaaca ataaaaaaat tatgggctct tggtctttgt ggcatattgg
    11641 taatcaatat gaattcaatg gcttacgctg atgttcttga tgatattata ggcagcagta
    11701 ctattgaaga tacggaagat gctgaaaaca ctgaagatgt atacacagat gttgataatg
    11761 aagacgtaac tggaaatgat atcacagtca ctgatgaaaa tatagcaaaa tattcggttt
    11821 tgttttccga cattgataat cattgggcaa aagaatggat taaaaatgca gttggtttag
    11881 gctttgtatc aggatatgaa gatggaacct tcaaaccgga cagaactatt acaagagctg
    11941 aattttcaac aatgttaaac aaggcaatga aaattgaaat aaccgaaaat ataaactttt
    12001 ccgatgtaag agaaaaggaa tggttttata aggaaattca aaaatctgta gctgccggat
    12061 ttttttcagg atatgaaaat aatacattca gaccaaacaa tcccatcaaa cgggaagagg
    12121 tagcaaaagt tgttgcaggt gccataacaa cgggaaacat tgacggtgaa ggtgctacgc
    12181 ttttaaagga ttacagtact attcaggaat gggcaaaaga tagtgtaaat actgtttaca
    12241 acaaaggata catactaggc tatcccgaca aaacgtacat gccttctaaa gcattaaccc
    12301 gtgccgaagc agtgaaaatt atctttgaaa tagttgataa tgaaaatatt gaatacgggt
    12361 ttaatattac aaattacaat gaaacttaca gcagcgcggt tgtggtggga aatttaaatg
    12421 ttttggattc tgtcggcaac ggtaacgtat atattaaaaa tgttgtggtt ttaggtgaca
    12481 ttgtaatttc ggctaaaaat gttaaatcgg ttgagcttac agatgttaag gcaagaagaa
    12541 taatagttga aggtgaaaat aatcctgtaa gagtagtgta caatgacaat gtaataattg
    12601 caaaaaaata gatataaaaa gattcttcat cacgaagaat cttttttgtt gccggtttat
    12661 ttcagcattt caagcgtatt tacaaaattc ttatttaaat taatttcatt tatataattt
    12721 ttatcaatat cagaaatttt ttcatatatt tctaatatat attcttgttc tcccgccata
    12781 accgccttat tgaaacgaga atcgtaattc attgacaact caataaaggc cttatcattt
    12841 tcaataaata aattttctat atcttttgtc aatgcacctc ttaaatcgtt taaataaaca
    12901 ctctttataa cattattaag attactaata tttttaaagt ctgcatacag atttcttccg
    12961 ctgtaatagt tctcatcgta aatataatag aatttttgct ttttataatc aattgaattc
    13021 agcaatttgt ttgctaactc atttttagtt tcatgctctt ttgtaagttt atctgttgca
    13081 gttaaagtat agtctcttat tattttactc ccatccttca tccaataagc tattacaaaa
    13141 ttatttattc cgtaataatc agattcatga taaaatgttt ggtcttttaa tacttcctcg
    13201 tgaagttcag taataatttc aattatatcc tcatcttcat aagtaatgat attcctgttt
    13261 cttttccatt ccggaaaatc tgaattttca cttgtatagt cgccccttaa aaacctgtcg
    13321 atttcattta ttgaccatct ttgattgccc acatatgcca tttgaacatc ttccggctca
    13381 ggaacaagat tttcatattg acttgcaacc cctacggtgc ctcccgttat tacagcaaat
    13441 gcaaccatgc tcactataaa caccttaact gaagatttgg atattctgaa tgatttttca
    13501 ataaatgcaa ttattatata gtaggatagt actgccaata atgctgaaat aattaacctg
    13561 tttccgatac tcatatatct tgaaaagcct attgaaaatg atgcaggtat tatcaaacat
    13621 gcaagcactg ccacaaaata tttaaatcca ttgaatacaa taaatccggt attttcgttt
    13681 tttctccttt ttataaattt atttataagc agtgatataa atattaatat agttggcaac
    13741 aaaagcaaat ataccaaatc tatcgattta ctttccgcgt accttacgaa gtacaaataa
    13801 tcaatcctgt atatattatt aacgaaataa tcgcttaaaa catccgcgct gaaccctaat
    13861 accaggtttt ccaatattga aaatataaac attataacca acagtattat caggggaagg
    13921 caaaagttaa atattgtcat tgctcctgcc atcaaggtat ttcctgacaa tattgacgac
    13981 aaagacgcta acagtattat tgccgaaagc cagggaataa gcattttaaa cactttaagt
    14041 acttcatagt ccagcccctg aaaaattacc gacaagacaa taagagccag taaataaact
    14101 gtaaaaactg catttgtcac tatgttaata attatctgtc tgcctaaggt cacaggcttt
    14161 gagctataat atgttaactt tgattcattg tggaaaaaac tgttatcaac cagacagttt
    14221 atcaaaacca aaacaaaaag ctcaactata ccaaacatta taaaaaattc ttcatctact
    14281 ctcaaaatat acggtattaa aagtgttgtt aaaaaaatta tagccgttga cagaagtacc
    14341 caatttttct tttccttaat tgaatatatt aatagcggca taaagttatt tttcttttcc
    14401 ataacccaca cctcctaatt ctgtaataaa tatttcttca agactcattg acagcttatc
    14461 gaacaacaaa agagtatatt tgctttccaa atatttttta agctcatcta tgtttcctgt
    14521 taaagttgca ataaatacgc tgccgatttt cacaacctga agcacattga attcttttag
    14581 cttatcaggg tcaaattcac cctccactgc aaattgaact cttttaatat cctctttcat
    14641 cttttcaagg ttttcttctc ttaatattct gccttcatgc attattccta ctctgtcgca
    14701 tatattatcc aattccttca ggtcgtggga agaaataacg acagtcatgt tattatcaat
    14761 tatttctttt ataagaacat cccaaaactt tcttttaatt actgcatcta atccatctac
    14821 aatttcatca agcaagagca gtctgggctg agccgctatt gacaaaacaa atgctgcctg
    14881 ctttttttga ccttttgaca tcttgtttaa tactttattt ttatctatgt taaagtactt
    14941 tagcaaatat tcaaattttt cttcagacat attatacatg agcttttcat aatggaataa
    15001 tttgttcaat gtatactgat agggaaagta cagcgtgtcc tgtacaaaat agaagtcttt
    15061 aataatatcc gagccttcca caacctcttc cccgttaaaa gttatagagc cgtcatcttg
    15121 tttgtagact ttcattatat gctttaatat agttgttttc ccgcagccgt tagagcctac
    15181 cagaccataa atactgcctt tatcggcatt tatgtttata ttttttaata cctgaaaatc
    15241 tccgaatttc ttgcttaaat tatttatggt taacatcatt gcctcctttt ttgcctggag
    15301 ttaaaatatc tctgaccatt tcaattattc tttcatctct taaccc
//
LOCUS       DAOPUE010000038        15196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3970_length_15196_cov_9.901328, whole genome shotgun sequence.
ACCESSION   DAOPUE010000038 DAOPUE010000000
VERSION     DAOPUE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15196
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_15196_cov_9.901328"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1840
                     /gene="dnaX"
                     /locus_tag="PKH42_05020"
     CDS             158..1840
                     /gene="dnaX"
                     /locus_tag="PKH42_05020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOA19825.1"
                     /translation="MSHQAIYRKFRPKIFDDVLGQEHVTRTLKNQIMSQNIAHAYLFS
                     GIRGTGKTSTAKIFARAVNCLNNHEGNPCNECEICISILDETNMDVIEMDAASNNGVD
                     DIRELRDKVMFLPVKSKYKVYIIDEVHMLSKGAFNALLKTLEEPPEHLLFILATTEPQ
                     KIPATILSRCQRFDLKRINADIIVENMKKICNELEIKYEEKALKLIAANSEGAMRDAQ
                     SILDRCISFNSQVDYDTVINLLGTVNYQVILEAVEAIADKNIVKTMSLVDNILNSGKE
                     LTFFLDELIICFRNMLIIKTTNSSDNLMRISEDETNEIKELNKKISVEEIVKIIEELS
                     IAQSECKKALNPRVLLETKLIKMLHNVHYVNLDVLVERIENIESSINGKYQPKDAVKE
                     PESTENIERTIKTTSVKKTEKEDILKTEKIENVDKNKEEALENKQQNKSDQAIFDAII
                     SEWQSLLKEIRRENAGLQAIIRESKLTEVINKKAVFEFDSKFTFHINAANQPKNKEEF
                     KEILSRFLNEKCEVEFITKKEIKSVKPKTTIKDVYEDLKNEFGEIVEYKQED"
     assembly_gap    1931..2030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2031..2561
                     /gene="recR"
                     /locus_tag="PKH42_05025"
     CDS             <2031..2561
                     /gene="recR"
                     /locus_tag="PKH42_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOA19826.1"
                     /translation="RKSAQRLAFHILEMKSSDVIELAKAIVNAKKYTKHCQRCGNLTE
                     GELCGICRSLKRDSSTICVVEDARDISAMERTKEYKGVYHVLHGTISPLDGIGPDDIN
                     IKSLISRLNTDIKEVILATNPTIEGEATAVYISRLIKPMGIKVTRIAHGIPVGGDIEY
                     ADEVTLMRAMEGRREM"
     gene            2666..3715
                     /locus_tag="PKH42_05030"
     CDS             2666..3715
                     /locus_tag="PKH42_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOA19827.1"
                     /translation="MNKKDEPESDSKFKSFLRRKNIEISAQRYLIEALGLMAYGLFAS
                     LLIGTILNTIGKKFGISFLTETVWPICSQMTGAAIGVAVAYGLKAPPLVLFASTITGT
                     AGNILGGPAGAFCSALVGAEFGKLVSKETKIDIIITPAVTIIAGVLIASLVGPAIGAF
                     MSATGEFIMYATTLRPFLMGILVSVVVGIVLTLPISSAALCMMLGLSGIAAGAATAGC
                     CAHMVGFAFLSFKENGWGGVAAQGLGTSMLQMPNLVRNPRCWIPAVIVSAITGPMATV
                     LFHMENSPIGAGMGTSGLVGIIETLNTMGGGMKVWLGILILYILIPVVLTPLIGNILR
                     KVGWIKDGDLKLDLV"
     gene            3815..4453
                     /locus_tag="PKH42_05035"
     CDS             3815..4453
                     /locus_tag="PKH42_05035"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOA19828.1"
                     /translation="MAQLYFRYSTMGAGKSIEVQKVAFNYEERGQNPLIFTSSIDNRF
                     AESGIVASRIGLSRRAIPVYDDMNIFEVIKEYNDIFGVDAVIIDECQFMKKHHIQELT
                     DVVDELNIPVMCYGLRADFRNELFEGSYWLFALADKIEEIKTICWCGKKALTNARIVD
                     GKVVYVGEQIFIGGNESYMPLCRKHYKEGKIMPYREKGEDLIIRYEKLKKEK"
     gene            4493..4822
                     /locus_tag="PKH42_05040"
     CDS             4493..4822
                     /locus_tag="PKH42_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOA19829.1"
                     /translation="MAIFEDFMKLDIRVGEIVEAKNFEKARKPAYKLIVDFGHEIGTK
                     KSSAQITECYTVDELIGKQVLAVVNFPPRQIADFISEVLVLGVYTEKGVVLIKPDQRV
                     EKGAKLG"
     gene            4849..5313
                     /locus_tag="PKH42_05045"
     CDS             4849..5313
                     /locus_tag="PKH42_05045"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOA19830.1"
                     /translation="MKSVFYYDTDIGRIGIAEDGNFITNVFFENEELEDIKINETPLI
                     KEAHKQIEEYLRGERKSFDLPISASGTEFQQRVWNALKTIPYGKTVSYKDIAIAIGNE
                     KSCRAVGMANNRNPISIIIPCHRVIGKNGKMVGYGGGIEIKEYLLKLENNNL"
     gene            5399..5644
                     /locus_tag="PKH42_05050"
     CDS             5399..5644
                     /locus_tag="PKH42_05050"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HOA19831.1"
                     /translation="MNDVQRKISIKSIIESFKKNKSADEEMFKNIENAKREWEDAKNI
                     FENVSHPDLVDYAIYKVEAAEQKYIYLLKQFKSNNLT"
     gene            complement(5713..5955)
                     /locus_tag="PKH42_05055"
     CDS             complement(5713..5955)
                     /locus_tag="PKH42_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOA19832.1"
                     /translation="MVVREAVINRINEICDDRGIAVNKLANLSGITPSTVYSIFNSGR
                     KDIGIVLIKKICDGFDITLDEFFSSDLFKSLEQELL"
     gene            6161..7249
                     /locus_tag="PKH42_05060"
     CDS             6161..7249
                     /locus_tag="PKH42_05060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOA19833.1"
                     /translation="MLKWSKYRKSDDMPPIAVEQIIKKRDKLYELDYMRFIACFAVMI
                     VHITATGVSEYIHGTFPYILMLTVNRSLKFTTPVFILLSGVTSFYSYSNKNREFKYME
                     FLKKRLAKVIVAYLIWCIIYYAAYIYFGYYAIDIKFFVKSVLQGTMSYHFYFVIIIIQ
                     MYIVGPIFYFILKNSGKKVTILIFAAVITYLCAEFIRFDLSDRIFLKYIVFYMLGIYI
                     AMEYDNYSLWINKNKLYIILGYIMMSIIYIVVSYYDSVASTFAWFIFSTMSLFFVYLI
                     GLLMKDKLKNIYGFIEVFGQSSYYVYLMHPLVLTIMILFAEKNFLSVTDKIILNTFTV
                     IPVTVISCLTYTFIKNKFKKKEKTLAVN"
     gene            complement(7275..7838)
                     /locus_tag="PKH42_05065"
     CDS             complement(7275..7838)
                     /locus_tag="PKH42_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOA19834.1"
                     /translation="MNITKEKAWELLKKYNKDEFHLQHADTVSFVMEYFAKKLGFEDE
                     AEFWGIVGILHDIDFEMYPDEHCVKMQEILSQNGVDEKIIKSAASHGYGLTDVTIKPE
                     HTMEKILFATDELTGLIGASALMRPSKSTLDMELKSLKKKFKSPGFAAGCSREAIKTG
                     AEMLGWELDYLLDETLKAMQEYEKARN"
     gene            8129..8893
                     /locus_tag="PKH42_05070"
     CDS             8129..8893
                     /locus_tag="PKH42_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOA19835.1"
                     /translation="MKDITRVQSIDRAVAILDCFSEEKKELKLSEISERLGLNKSTVH
                     GIISTLKYHGFISQDEETQKYKLGIRFVQFGDLVINSMNIRNAAVPVIDAVCEKIEET
                     VHVAMLDGLDVVWIEKRECTKSIKTSTKIGARLPAYTTADGKIIICYQNKDKIKNYLP
                     KRIPQYTNNTITNKGEFIKKLEEMKKNGYAIDNEEYVEGLKCVAAPIFDHDGKVRFSL
                     STTGPAFRMNEERIKELIVIIKEAANEISQRIGYMK"
     gene            9218..10303
                     /locus_tag="PKH42_05075"
     CDS             9218..10303
                     /locus_tag="PKH42_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19836.1"
                     /translation="MKKLLAIVLILAMSLFAFVACTQDQPGETPKNEEPKTEEPKTEE
                     PKEEEPLAEGAVKTGLAVISSVEKSKDAGEKDGLAQTDSVIVAVTVDKDGKIVQTIID
                     TAQTKIEFSKEGKIIPDLNTVFKSKQELKEEYGMGKASSIEKEWYEQANALAEYVKGK
                     TVEEVKAIAVDETGVTTEADLVSSVTIKINPYKEAIEKAVANAENLGADASDKLGLGV
                     ITTIAKSKDATADAEGLAQAYSTYTVATFDADGKVTSSIIDASQANVKFDNTGKITTD
                     LAVAPLTKVELGEDYGMKKASAIGKEWYEQADAFADYVVGKTVEEIKGIAVDESGHAT
                     EEDLVSSVTVGIVDLKTIFEKAYNNSI"
     gene            10377..11423
                     /locus_tag="PKH42_05080"
     CDS             10377..11423
                     /locus_tag="PKH42_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOA19837.1"
                     /translation="MAKKIISVILLILIIISLTGCSDKEPIRYQSEFIMLFDTVTRIV
                     GYSKSKEEFTEYAQIIYDNLKEYHQLYDIYNDYEGINNIKTINDNAGIKPVKVDKRII
                     DLIKFSKDMYEKTNGKTNIAFGAVLRIWHEYRTIGIEDPANAALPPEEELRIASEHTN
                     IEDIIIDEEQSTVFLRDPFMSLDVGAVAKGYATEQVSKIAKESGMNSALLSVGGNVRA
                     INKNVTSNEPWNVGIQNPDTEAEESVIKIVKLDDSSLVTSGDYERFYIVDGEKYNHII
                     NPDTLYPAKYFTAISIICKDSGLADALSTAVFCMPFEEGLEFINSLPEAEAMWIFSDK
                     TIKYSDNFESFIKK"
     gene            11518..13323
                     /locus_tag="PKH42_05085"
     CDS             11518..13323
                     /locus_tag="PKH42_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19838.1"
                     /translation="MKKESELKIIPILSKGFKRFFFISAAATILKVSASFAVPQVIRF
                     TVDHIIDDYPLNYIQLVMNTINNIGGIEFLKENLWLLTFFILVLAVITGVSDYIGKIT
                     LAKGTEGIIKNIRDKLFEHIQKLPYSWHIKNQTGDIIQRSTSDVEVVRNFISTQLVEI
                     FRVIMLVIISLILMFSMNFHLTAAVLVFIPVVVLYSTIFYGILTKKFLVADEAEGKLF
                     TAIQENLTGVRVVRAFGREKHEIEKFTERNNTFSELWVKLGYQLGYYWGIGDLVTGVL
                     LMVIVVYGTIFAINDRITLGEFLAFASYTVMISWPLRSFGRILSELSKAKVSLKRICE
                     ILKEPVEDDSPDSVTPDILKDIEFKNVTFKYDGETEVLKNVSFTIKAGSTFAILGGTG
                     SGKTTLMYLLNRLYDLPDHMGCITIGGVSIKNIKREHLRKNIGMVLQEPFLFSRTIKE
                     NIAIVEPDTSLDEIKKAASIAQIDESIEEFSKGYDTIVGERGVTLSGGQKQRVAIARM
                     LLQKTPIMVFDDSLSAVDADTDAKIRSSLIKSKGSSTIIIISHRITTLMEADTILVLD
                     KGEVSDIGTHEELINRDGIYKDIFNVQMNLSEDIE"
     gene            13320..15143
                     /locus_tag="PKH42_05090"
     CDS             13320..15143
                     /locus_tag="PKH42_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19839.1"
                     /translation="MNYYEEESYSSFNIKPWLKLGRFFKPYTGKLLGIIFFMFITALM
                     DLSIPLFQKYAVDSFIAKNTTEGINGFIALYTAVLVIMAISVNIFIRLAIVVEMHIGM
                     DLRKEIFEHLQNLSLSYYNTTPVGYIMARTLSDTNKIGSMIAWGLVDVFWSFAYVTGA
                     FIAMLLLNVQLTVIIAIVIPVMAVITVYFQNKILLTSRKLRKINSKMTGSFNEGIIGA
                     TTSKTLVIEDKNLVQFKNISSEMKMTAVNLARLNALYIPIIVFFSSVLTALVLAKGGR
                     LAMDKIIQIGTLSAFLSYAISIFEPVQQLARTFAEVISLQANIERVTDLLEKEPLIKD
                     SEEVVKIYGDTINPKKENWEEIIGEIEFKNVSFKYPDGDEEVLQNFNLHIPAGTNVAI
                     VGETGAGKSTLVNLACRFFEPTEGEILIDGKDYRERSQLWLHSNIGYVLQDPHLFSGT
                     IKENIRYGKLDATDEEIMEAAKIVSVDTIINKLEKGYDSHVGEGGDRLSTGEKQLISI
                     ARAILSNPKIFILDEATSSVDTQTEILIQKGIEYLLKGRTSFIIAHRLSTIKKADIIL
                     VVNEGKIIEQGTHNELIAKRGHYYNLYTKQFEEEKTLEVLK"
BASE COUNT         5484 a         2095 c         2961 g         4556 t
ORIGIN      
        1 gaatgatgta cttaaggcaa tttaggggaa accttaaatt gctttttagt ttggagacat
       61 tccttttttc aatatccctt attgtttaaa aaaatatttt atgctataat gaatacgaaa
      121 aatgaagcaa aaaaattaca gggttcaggt ggtgtatatg tctcatcaag caatatacag
      181 aaaattccgt cctaaaatat ttgatgatgt cttgggtcag gagcatgtga ccagaacatt
      241 gaagaatcaa ataatgtctc aaaatatagc ccatgcttat cttttcagcg gaataagagg
      301 caccggaaaa acatccactg ccaagatatt tgcaagagct gttaactgtt taaacaacca
      361 tgaaggaaat ccatgcaatg aatgcgaaat atgtataagc attttggatg aaaccaatat
      421 ggacgtaatt gaaatggatg cagcatcaaa taatggcgta gatgatatta gagagcttag
      481 agacaaggta atgtttttgc ctgttaaaag caagtacaag gtatacataa tagatgaagt
      541 acatatgctg tcaaaaggcg cctttaatgc gcttttaaaa accttggaag agccgcctga
      601 gcatttatta ttcattcttg caactacgga gccgcaaaaa attcctgcta caatattatc
      661 caggtgccag agatttgact taaagagaat caatgctgat attattgtag aaaatatgaa
      721 aaaaatctgc aatgaattag aaataaagta cgaagaaaaa gccttgaaat tgattgcagc
      781 aaattctgaa ggagcaatga gagatgctca aagtattttg gacagatgta tatcctttaa
      841 ttctcaagtt gattatgata ccgtaataaa tttattgggg acagtcaatt atcaggttat
      901 tttggaagca gttgaagcaa tagcagacaa gaatattgta aaaaccatgt ctttagttga
      961 caatatttta aacagcggaa aagagcttac attttttctt gatgagctta ttatttgttt
     1021 tagaaatatg cttataatta aaacgacaaa ttcttccgat aatttaatga gaatatctga
     1081 agatgaaaca aatgaaatta aagaattaaa caaaaaaatc agtgttgaag aaatagttaa
     1141 gataatagag gagctttcaa tagcacagtc tgaatgcaag aaggcattaa atccaagggt
     1201 tcttttggaa acaaagctta taaagatgct acataatgta cattatgtaa acctggatgt
     1261 attagtcgaa agaattgaaa acatagaaag cagcataaat ggaaaatatc agcctaaaga
     1321 cgcagtaaaa gagccggaaa gcacagaaaa cattgaaaga acaataaaga caactagtgt
     1381 aaaaaaaacc gaaaaggaag atatattaaa aaccgaaaaa attgaaaatg tagacaaaaa
     1441 taaagaagaa gctcttgaaa ataagcaaca aaacaaaagc gaccaagcaa tttttgatgc
     1501 cataattagt gaatggcagt ctttacttaa agaaatcagg agagaaaatg caggcttgca
     1561 agctattatt agagagtcca agctgactga ggtaataaac aaaaaagctg tatttgaatt
     1621 cgactctaag tttacattcc atattaatgc tgcaaatcag cctaaaaaca aagaagaatt
     1681 caaagagata ttaagccgtt ttttaaatga aaaatgcgaa gttgaattta ttacaaaaaa
     1741 agaaataaaa agtgtaaaac caaaaacaac tataaaagat gtatatgaag atttaaaaaa
     1801 tgagtttgga gaaatagtag aatataaaca ggaggattga aatggcaaaa ggcagaaaaa
     1861 tgcccatggg tatgcccgga ggcgggaata tgaacaatat gatgaaacag gttcaaaaaa
     1921 tgcaaagaca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agaaaatctg
     2041 ctcaacggct tgcatttcat attttggaaa tgaagagcag cgacgttata gagttggcaa
     2101 aagcgatagt aaatgcaaaa aagtatacaa aacattgcca aaggtgcgga aatttaacgg
     2161 aaggcgaact gtgcggcatt tgccgcagtt taaagaggga cagctcaacc atatgtgttg
     2221 ttgaagatgc aagagatatt tcagctatgg agcgaaccaa ggaatacaaa ggagtctatc
     2281 atgttcttca tgggacaatt tctcctcttg atggaatcgg tcctgatgat ataaatatta
     2341 aaagtttaat atcaaggctt aatacagata taaaagaagt aattttagca accaatccta
     2401 caattgaggg agaagcaacg gctgtttata tatcccgcct cataaaaccc atgggaataa
     2461 aggtaacacg catagctcat ggaattccgg taggcggaga catagaatat gccgatgaag
     2521 taactcttat gagagcgatg gaaggcagaa gagaaatgta aaaagaattc cggggacgga
     2581 cctcaaaatt ccggtaatac tgggatgaag tcaccggaag gtccgttccc gtaattatgg
     2641 aataaaaata acaagaggga tgagtatgaa taaaaaagac gaaccggaaa gcgattccaa
     2701 atttaaaagt tttctaagaa gaaaaaatat tgaaatttct gctcaaagat acctgattga
     2761 agcattgggt ttaatggctt acggtctttt tgcatctttg ctgataggta caattctcaa
     2821 tactatcggc aaaaagtttg gcatttcttt tttaaccgag acagtatggc ctatttgcag
     2881 tcagatgacg ggagctgcaa taggtgttgc cgtagcttat ggactaaagg ctccgccttt
     2941 agtcttgttt gcatctacaa ttacaggaac ggcaggtaat atcttaggtg ggcctgcagg
     3001 tgctttttgc agtgctttgg tgggtgctga atttggtaaa ttagtttcaa aagaaacaaa
     3061 aatagatatt attattactc cggctgttac tattattgca ggtgttttaa ttgcttcatt
     3121 ggtaggacct gctataggag catttatgag tgcaacgggt gaatttataa tgtatgctac
     3181 aaccctgagg ccttttttaa tgggtatatt ggtttcagtt gtggtaggca tagtattaac
     3241 actgcctata agcagtgcag ctctttgtat gatgcttggt ctttcaggca tagccgccgg
     3301 cgcagcaact gcaggatgct gtgcacatat ggtaggtttt gcttttttga gttttaagga
     3361 aaacggctgg ggaggagttg cagctcaagg attgggaaca tcaatgctgc agatgccgaa
     3421 tcttgtgagg aatcccaggt gctggattcc tgccgtaata gtatctgcca ttacgggtcc
     3481 catggcaacg gttttattcc atatggaaaa cagtcccata ggtgccggta tgggaaccag
     3541 cggcttggta ggaataattg aaacattaaa taccatgggc ggagggatga aggtgtggtt
     3601 gggaatacta atactataca ttttaattcc ggtggtactc actccgctta tcggtaatat
     3661 tttaagaaag gtcggttgga ttaaagatgg cgatttgaag ttagatttag tctagcctaa
     3721 tgtatgaccc ttaatccgta tattaggttt acataattga ttttatgtaa accttagtga
     3781 taaaagaaag taaaaaataa tatagaggag agttatggca caactttatt tcagatacag
     3841 caccatgggt gctggaaaga gtatagaagt acaaaaggtt gcatttaatt atgaagaaag
     3901 aggacaaaac cccttaatat ttacatcttc aattgataac agatttgcag aatcaggaat
     3961 tgttgcttcg agaatcgggc tgagcaggag agcaatacct gtttatgatg atatgaatat
     4021 ttttgaagtc attaaagagt ataatgatat attcggtgtt gatgccgtaa taattgacga
     4081 atgtcaattt atgaaaaaac accatataca agagcttacg gatgtagttg atgaattaaa
     4141 tatacctgtt atgtgctatg gattgagagc tgatttcaga aatgaattat tcgaaggctc
     4201 ttactggctt tttgcattag ctgataaaat tgaagaaatt aagactattt gttggtgcgg
     4261 caaaaaagca ttaactaatg cccgtattgt ggacggtaaa gtagtatatg tgggcgaaca
     4321 aatttttata ggaggcaatg aaagttacat gcctctttgc aggaagcact ataaggaagg
     4381 aaagattatg ccttacaggg aaaaaggcga ggatttaata ataagatatg aaaagcttaa
     4441 aaaagaaaaa tagatatgat tactcaatag tgattatata aggagatatc atatggcaat
     4501 atttgaagat tttatgaagt tagatatcag ggtgggagaa atagtagaag caaaaaactt
     4561 tgaaaaggca aggaagcctg cttataaact gattgttgat tttggacatg aaatcggaac
     4621 taaaaaatca agcgcccaga ttacagaatg ttatacagtt gatgagctta taggcaaaca
     4681 ggttttggca gttgtgaatt ttcctccgag acaaattgcg gatttcatat ctgaagtgct
     4741 agtgcttggg gtttacacag aaaagggagt agtcttgatt aagcctgacc aaagagtaga
     4801 aaaaggtgcc aaattaggat aaagagaggt ataataagag gaggaattat gaaaagtgta
     4861 ttttattatg atacggatat cggcagaata ggtattgcag aggatgggaa tttcataacg
     4921 aatgtttttt ttgaaaacga ggagcttgag gatattaaaa taaatgaaac tcctttaata
     4981 aaggaagctc ataaacaaat agaagaatat ctgagaggtg aaagaaaatc ttttgatttg
     5041 ccgatatctg catccggtac cgaatttcag caaagggtgt ggaatgccct taaaactatt
     5101 ccttacggaa agaccgtaag ctataaggat attgcaatcg caataggaaa tgaaaaatcg
     5161 tgcagggctg taggaatggc aaacaaccgt aatccaattt caattattat tccctgtcat
     5221 agagtaatag gaaaaaacgg caagatggta ggatacgggg gaggcataga aataaaagaa
     5281 tacctgttaa agcttgaaaa taataattta tagttataca taatgcaaat tatttttgga
     5341 atattaggaa taaaaaggaa cttcctgtct ataataataa ggataggagg ttttttccat
     5401 gaatgatgtt cagagaaaga ttagtattaa aagtataata gagagtttta aaaaaaataa
     5461 atccgcagat gaggaaatgt ttaaaaatat tgaaaatgca aaaagagagt gggaagatgc
     5521 aaaaaacata tttgaaaatg tttcacatcc tgatttagtg gactatgcaa tatataaggt
     5581 tgaagctgca gagcaaaaat atatatatct tttaaaacaa ttcaagagta ataatttaac
     5641 ataagaaggc ggaatattaa ggggggacac ttttaagcgt ccctcttctt tctttatctt
     5701 tcatatcttc ctttataata attcctgctc taaggactta aacaaatctg aagaaaagaa
     5761 ttcatccaat gttatatcaa atccatcaca aatctttttt atcagcacaa ttccgatatc
     5821 tttcctgccg ctgttaaata tactgtagac agtagatggt gtaattcccg ataagtttgc
     5881 aagcttattt acggctatgc ccctgtcatc acaaatttca tttattctgt ttattacggc
     5941 ttctcttaca accatgtcgt cacctccaat gaatacttaa taaaacaatt atagcacaat
     6001 ttcgcaatta cgtatacgca aaaacgtaga tttttaataa aattgcaaat aaatttataa
     6061 aataacgcta ttatgtatct tttgtcatta ataatgaata attatgatat atgtgaatat
     6121 atatatgaat ttctaaaata attgattttc ttctgtataa atgttaaaat ggtcaaaata
     6181 tagaaagagt gatgatatgc cgccaattgc agtcgagcaa attataaaaa aaagagacaa
     6241 gctttatgaa ttggattata tgagatttat tgcatgtttt gcagttatga tagtccatat
     6301 aacagcaacg ggagtttctg aatacattca tgggacattc ccctatattt tgatgcttac
     6361 cgtaaataga agtttaaagt ttactacacc cgtctttatc ctgttaagcg gagtgacaag
     6421 cttttacagc tacagtaata aaaaccgtga atttaagtat atggaatttc tcaaaaaacg
     6481 acttgccaaa gtaattgttg catatttaat atggtgtatt atttattacg cggcttatat
     6541 atattttgga tattatgcga ttgatattaa attttttgtg aaaagtgttt tacagggaac
     6601 aatgtcatat cacttttatt ttgtaataat tattatacaa atgtacattg taggtcccat
     6661 attttatttt attttgaaaa attccggaaa gaaagtgaca atccttatat ttgctgcagt
     6721 aataacatat ttgtgtgctg aattcataag atttgatttg tcggacagga tatttttgaa
     6781 atacattgtg ttttatatgt tgggtatata tattgctatg gaatacgata actattcttt
     6841 atggattaat aaaaataaat tatatattat attagggtat attatgatga gtataattta
     6901 catcgttgtt tcatattatg attctgtggc atctactttt gcttggttta tattttcaac
     6961 tatgtctttg ttttttgtat atttaatagg actattaatg aaggataaat taaaaaatat
     7021 ttacgggttt attgaagtat tcggacaaag ctcttactac gtttatttaa tgcatccttt
     7081 ggttttgact attatgatac tatttgcaga aaaaaacttt ttatctgtta ccgataaaat
     7141 aattctcaat acttttactg ttatacccgt cactgtaatt tcttgtttaa catacacctt
     7201 tataaaaaac aaatttaaga aaaaggaaaa aacactagct gtaaattaaa aaggatatgc
     7261 atggcatatc cgttttaatt tcttgctttt tcgtattcct gcattgcttt taacgtttca
     7321 tccaacaaat agtctaattc ccatcccaac atttcagcac cggtttttat tgcttctctg
     7381 gagcagcctg cagcaaagcc gggtgatttg aattttttct ttaatgactt aagctccata
     7441 tccaatgtac tctttgaagg acgcatcaat gcagaagcac ctatcaatcc tgtcagttca
     7501 tcggttgcaa aaagtatttt ttccatagta tgttcaggtt ttattgtaac atctgtaagt
     7561 ccgtatccat ggcttgcagc tgatttaata attttttcgt ctactccgtt ttgggacagt
     7621 atctcctgca tttttacgca atgttcatca ggatacattt caaagtcaat gtcatgcaaa
     7681 attcctacaa ttccccaaaa ttctgcttca tcttcaaaac ccagtttttt cgcaaaatat
     7741 tccattacaa agcttacagt atctgcatgc tgcagatgaa attcatcctt gttgtacttt
     7801 ttaagcaatt cccatgcttt ttctttagta atattcatta tatcccttcc ttataattta
     7861 atatcataat tttaacttat ttaacataac atgtctataa caaaaaattt tttatttgac
     7921 aaattttcat acagattgac aaataattta catagtgata tagtatcatt ataaattgta
     7981 ttgtagtatt taaacattga aattaaaata cttatagcat aatattaaaa aaattaataa
     8041 ataattggat actgcacaat tcgtttgtta ataaattatt tatattgatt tttttaaaat
     8101 cacatgctat aattttggag tatcgtttat gaaagatata acgagagttc aatccattga
     8161 cagggcagtt gcaatattag attgtttcag cgaagaaaaa aaagaactta agctgtcgga
     8221 aatttctgaa agattaggtt taaacaagag tactgttcat ggaattatat ctactttaaa
     8281 gtatcatggg tttatttctc aggatgaaga aactcaaaaa tacaaattag gtatacggtt
     8341 tgtccaattt ggagacttag taattaattc aatgaatata cgaaatgcag cggtacctgt
     8401 tattgatgca gtttgcgaaa aaattgaaga aacagttcat gtagcaatgc ttgacggact
     8461 ggatgttgtt tggatagaaa aaagagaatg caccaaatca attaaaactt caaccaaaat
     8521 aggagcaagg ctgccggcct atacaactgc agatggtaaa ataattatat gttatcaaaa
     8581 taaagataaa attaaaaatt atttaccgaa aagaattcct cagtatacta ataatactat
     8641 aacgaataaa ggtgagttca ttaagaaatt agaagaaatg aagaaaaacg gttacgcaat
     8701 tgacaatgaa gaatatgttg aaggattaaa atgtgtagct gcaccgatat ttgatcatga
     8761 tgggaaagta agattcagtt taagcacaac aggtcctgct ttcaggatga atgaggaaag
     8821 aataaaggaa ttaattgtta ttattaaaga agctgcgaat gaaatatcac aaagaattgg
     8881 atatatgaaa taaatttagg attgtcgggg aaccagatcc ttttaaaagc atcatacgcc
     8941 agaatgcagt ttggcaataa tgaacataag ttgaaaaaat aagtgttaac taatataata
     9001 caattgagat aattttgagg attgttggga aaccaatcct tttaaagaag cttatgtcag
     9061 aacgcagttt gttaatgatg aacattagtt aaaaaatttt caaaaaagtg ttgactaatg
     9121 taatacaatt tgttaatatt gtaccgattt aatcacaaag ttataagcag ctaaatgctg
     9181 attatatata tagattaaga atttatggag gaaagtaatg aaaaagttat tagcaatagt
     9241 gttgatctta gcgatgtctc tatttgcttt cgtagcatgt acgcaggatc aaccgggtga
     9301 aacaccaaaa aatgaagaac caaaaactga agaaccaaag actgaagagc ctaaagaaga
     9361 agaaccatta gctgaaggag cagttaaaac tggtttggca gttatatcat ctgttgagaa
     9421 atcaaaggat gcaggcgaaa aagacggatt agctcaaaca gattcagtaa ttgttgcagt
     9481 tacagttgac aaagacggaa aaatagtgca aactataatc gatactgcac aaactaaaat
     9541 tgaattcagc aaagaaggta aaattatacc tgatttaaat actgtgttta agtctaaaca
     9601 agagcttaaa gaagaatacg gaatgggtaa agcttcaagc atagaaaaag aatggtatga
     9661 acaagctaat gctttggctg aatatgtaaa aggcaagact gttgaagaag ttaaagctat
     9721 tgcagttgat gaaactggag ttacaacaga agctgactta gtatcatccg taacaatcaa
     9781 aattaatcct tataaagaag ctattgaaaa agctgtagca aatgctgaga atttaggtgc
     9841 tgatgcttct gataaattag gattgggagt tataactaca attgcaaaat caaaagatgc
     9901 aacagctgat gcagaaggat tagctcaagc ttactcaact tatactgtag caacatttga
     9961 tgcagacgga aaagtaacaa gctcaataat agatgcatct caagctaacg taaaatttga
    10021 taatacaggt aaaataacta cagacctagc agttgcacct ttaactaagg ttgagttagg
    10081 cgaagattac ggaatgaaaa aagcttcagc aataggcaaa gaatggtatg aacaagcaga
    10141 tgcatttgca gattatgtgg taggcaagac tgttgaagaa attaaaggta tagcagttga
    10201 tgaaagcggc catgctaccg aagaagattt agtatcatct gttacagttg gaattgtaga
    10261 cttaaaaacc atttttgaga aggcttataa caatagtatt taacaaagta taacaatcaa
    10321 agcgaatcat tatctaaggg gcagatgctg ccccttgttt ttattagagg tgtaagttgg
    10381 caaagaaaat catttccgta atattgttaa tattaataat aataagttta accggatgca
    10441 gtgataaaga acctattcgc tatcaatccg aatttataat gctgtttgat actgttacaa
    10501 gaattgtggg atattcaaag agcaaagaag agttcactga gtacgcacag ataatttatg
    10561 acaatttaaa ggaatatcat cagctttatg atatatataa tgactatgaa ggcataaaca
    10621 atattaagac tatcaacgat aatgcaggaa taaagcctgt caaagtagac aaaaggataa
    10681 ttgatttaat aaaattttca aaggatatgt atgagaaaac aaacggtaaa acaaatatag
    10741 cttttggtgc cgtattaaga atttggcatg aatatcgaac catcggtatt gaagaccctg
    10801 cgaatgcagc tcttccaccg gaagaagaat taagaattgc atctgagcac actaacattg
    10861 aagatataat tattgatgag gagcaatcaa cagttttttt aagagatcct tttatgagtc
    10921 ttgacgttgg tgccgtagcg aagggatatg caaccgagca ggtaagtaaa atagcaaagg
    10981 aaagtggaat gaattccgca ttattgtccg taggaggaaa cgtacgagcc ataaataaaa
    11041 atgttacctc gaatgaacca tggaatgtag gaatccaaaa tcccgatacc gaagctgaag
    11101 aatcagttat taaaatagtt aagttagacg acagctcttt ggtaacaagc ggtgattatg
    11161 aaagatttta tatagttgac ggggagaagt acaatcatat aattaatcct gacacattat
    11221 atcctgcaaa atattttact gccatttcca taatatgcaa ggattcggga ttggctgatg
    11281 cattatcaac agctgttttc tgcatgcctt ttgaagaggg tcttgaattt ataaattcat
    11341 tgcctgaagc ggaagcaatg tggattttca gcgataaaac aataaaatac agcgataatt
    11401 ttgaaagttt tattaaaaaa taaagcatga gtttaaattg acactaaatg aataataata
    11461 tagaattaat aagcaatcct gatggattgg ttatttattt ttttatttga ggataatatg
    11521 aaaaaggaat cagagcttaa gattatacca atattatcaa aaggatttaa aaggtttttc
    11581 tttatatctg ctgcagcaac tatactaaag gtttctgcaa gttttgcagt gcctcaagtt
    11641 attaggttta ccgtagacca tatcatagac gattatccct tgaactacat tcaattggtt
    11701 atgaatacaa taaataatat aggcgggata gaatttttaa aagaaaattt atggctcctt
    11761 acttttttta tattagtctt ggcagttata acgggagtaa gcgattatat cggaaaaatt
    11821 acccttgcta agggaacgga aggaattata aaaaatataa gggataagtt gtttgagcac
    11881 atacaaaaat tgccatacag ctggcatatt aaaaatcaaa caggagatat aatacaaagg
    11941 tcaacatcag atgtagaagt agttcgtaat tttatcagta ctcaattggt cgaaatattc
    12001 agggtaatca tgctggttat aatatcgttg attttaatgt tttcaatgaa ttttcacctt
    12061 actgcggcag tattggtatt tatacctgta gttgtgctat attcaacgat tttttatggt
    12121 atattgacca aaaaattctt ggttgcggat gaagccgaag ggaagctttt tactgcaatt
    12181 caggaaaatt taacaggtgt tcgtgttgtt agggcctttg gcagagaaaa acatgaaata
    12241 gagaaattta cggaaagaaa taatacattt tcagagctat gggttaagct gggatatcag
    12301 ttaggatatt attggggaat cggcgatttg gtaaccgggg tactccttat ggtcattgtt
    12361 gtatacggta ctatatttgc tataaatgat agaattacac ttggagaatt tctggcattt
    12421 gcttcttata ccgtaatgat ttcatggccg cttcgcagtt ttgggaggat tttatcagag
    12481 cttagcaaag ctaaggtttc attaaaaaga atttgtgaaa tattaaagga acctgttgag
    12541 gatgacagtc cggattccgt cactccggat atcttaaagg atattgaatt taaaaatgtt
    12601 acatttaaat atgatggaga aactgaggtt ttgaaaaatg tcagctttac aataaaggca
    12661 ggaagcacat ttgctatatt aggaggtacc ggctccggaa aaactactct tatgtatctt
    12721 ttaaaccgct tgtatgattt gcctgaccat atgggctgca ttacaatagg cggagtgagt
    12781 ataaaaaata ttaaaaggga acatttaaga aaaaatattg gaatggtact tcaggagccc
    12841 tttttatttt ctcgaacgat taaggaaaat atagcaatag tcgagcctga tacttccttg
    12901 gatgaaatta agaaagctgc atcaatagcg cagattgatg aatctataga agaattttca
    12961 aaaggctatg atacaattgt tggagaaaga ggagtaacat tatcgggagg tcaaaaacaa
    13021 cgggtggcaa tagccaggat gctgcttcaa aaaacaccga ttatggtgtt tgatgattca
    13081 ttgtctgcag tcgatgccga cacagatgca aaaattcgtt catcacttat aaaaagcaag
    13141 ggcagttcga caattataat tatttcacat cgcataacaa ctttaatgga agctgatact
    13201 attttagtct tggacaaggg agaagtatcc gatataggaa cccatgaaga attaataaac
    13261 agagatggta tttataaaga catattcaat gttcagatga acctatcgga ggatatagaa
    13321 tgaattatta tgaggaagag agttatagct catttaatat aaagccttgg ttaaaattag
    13381 gaagattttt taaaccctac acagggaaac tgttgggtat tatctttttt atgtttatta
    13441 ccgccctaat ggatttatcc attcctttgt ttcaaaaata tgccgtggac agttttatag
    13501 caaagaatac tactgaaggt ataaatggtt ttattgctct ttatacagca gtcttagtga
    13561 ttatggccat atccgtaaat atatttatac gattggcaat agtagttgaa atgcacattg
    13621 ggatggatct aagaaaggaa atattcgaac accttcaaaa tctttcgttg tcatattaca
    13681 acacaactcc cgtgggttat ataatggcaa gaactttaag cgataccaat aaaataggaa
    13741 gtatgattgc atggggattg gtagatgtat tttggtcatt tgcttatgta acaggtgcct
    13801 ttattgcaat gcttttgctg aatgtgcaac ttactgttat tattgcgatt gtaattcctg
    13861 taatggcggt tataactgtt tattttcaaa ataagatttt attaaccagc agaaaattaa
    13921 gaaaaatcaa ttctaaaatg acaggaagtt tcaatgaagg gataataggt gcaacaacct
    13981 ccaagacatt ggtaatagag gacaaaaact tggtgcaatt caaaaatata agcagtgaaa
    14041 tgaagatgac ggctgttaat ttagcacgat taaatgcctt gtacattccc ataatagtat
    14101 ttttcagctc cgttttgaca gcattggtct tggcaaaagg aggcagattg gcaatggata
    14161 aaattattca aataggaaca ctgtctgctt ttctttctta tgcaataagc atatttgaac
    14221 ctgttcagca gttggcaaga acatttgcag aagtcatatc actgcaggct aatattgaaa
    14281 gagtaacgga tttattggaa aaggaaccct taataaaaga cagtgaagaa gtagtgaaaa
    14341 tttacggaga cactattaat ccaaaaaagg aaaactggga agaaatcata ggggaaattg
    14401 aatttaaaaa tgtcagtttt aaatatccgg atggagatga agaggttttg caaaatttta
    14461 atcttcatat tcccgcaggt accaatgttg caattgtagg tgaaacgggt gccggtaaaa
    14521 gcactctggt aaatcttgca tgcagatttt tcgagcctac tgaaggtgaa atactgatag
    14581 atggcaagga ttacagagaa agatctcagc tttggcttca ttcgaacatt ggatatgtgc
    14641 tgcaagaccc gcatttgttt tccggaacaa tcaaggaaaa cataagatac ggtaaattag
    14701 atgcaacaga tgaagaaata atggaagccg caaaaattgt ttcagtcgat accataatca
    14761 acaaattgga aaagggttat gacagccatg tcggcgaagg gggagacagg ctttctacag
    14821 gtgagaagca gttgatttcc atagcaaggg caatactttc aaaccctaaa atttttattt
    14881 tggatgaagc aacatcttcg gtagatacac aaacagaaat acttatccaa aaaggaattg
    14941 aatatttgtt aaaaggcaga acttcattta taattgctca cagactttca actattaaaa
    15001 aggcagatat tatattagtg gttaatgagg gcaaaataat agagcaaggg acacacaatg
    15061 aactgatagc taaaaggggc cattattata atttatatac taaacagttt gaagaggaaa
    15121 aaacccttga agttctaaaa tgaggcaaat tataacattg acacatgatt ttaattatgt
    15181 tatcattttt gtgctg
//
LOCUS       DAOPUE010000039        15151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_3987_length_15151_cov_9.895800, whole genome shotgun sequence.
ACCESSION   DAOPUE010000039 DAOPUE010000000
VERSION     DAOPUE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_15151_cov_9.895800"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKH42_05095"
     CDS             complement(<1..254)
                     /locus_tag="PKH42_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOA19840.1"
                     /translation="MKNKIKITTIFLIIIMLLTTAAGCTGSNEKNLEIRVGGKNFTEQ
                     YIMAEMFSILIEENTDLNTTLQTNLASTVLFEAIKADEVD"
     gene            complement(274..906)
                     /locus_tag="PKH42_05100"
     CDS             complement(274..906)
                     /locus_tag="PKH42_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19841.1"
                     /translation="MQLLDYVQNNMNNIIRLTIEHLQITGISIITAIVIGVPLGIIVT
                     RYKRLADPILASANIFQTIPSIAFFGILIPFMGIGKETAILVLFLYALLPIIKNTYTG
                     INEVSPSLIDAGRGMGMTNFQILRMVELPQSLAVIMAGVRIATVINIGSATIAAYIGA
                     GGLGELIFKGIQMYRNDMILVGAILAALLAVVADKLLGYVEHKLTPRGMK"
     gene            complement(933..>1334)
                     /locus_tag="PKH42_05105"
     CDS             complement(933..>1334)
                     /locus_tag="PKH42_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOA19842.1"
                     /translation="FIGKDRLLKRPEYIKVKDIMIKKPVTIRPERTLTQALEKMRLHK
                     VDSLMVVDEFGNFIGIATSNDILDNFGNGGTVIEITRTEFNHVNEDANVNQVLSIMAQ
                     GQVGYVPVIDDEKHLLGLVTRSSLVNVLGRA"
     assembly_gap    1335..1434
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1435..1763)
                     /locus_tag="PKH42_05110"
     CDS             complement(<1435..1763)
                     /locus_tag="PKH42_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA19843.1"
                     /translation="MISFKNVTKKYGDSVAVDNLTVNFKSGETVVLIGPSGCGKTTTL
                     RMINRLIEPTEGSIYINDTDISKINPVELRRNMGYVIQQIGLFPHMNIAQNVGLVPYL
                     KDWSESKR"
     regulatory      complement(1894..2028)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(2103..3099)
                     /gene="guaA"
                     /locus_tag="PKH42_05115"
                     /pseudo
     CDS             complement(2103..3099)
                     /gene="guaA"
                     /locus_tag="PKH42_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965987.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
     assembly_gap    3224..3323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3341..4789)
                     /gene="gcvPB"
                     /locus_tag="PKH42_05120"
     CDS             complement(3341..4789)
                     /gene="gcvPB"
                     /locus_tag="PKH42_05120"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOA19844.1"
                     /translation="MQLIFEKSKKGRKAYSLPECDVPDVSLDVIDKKYLRAEAPELPE
                     VSELDAVRHFTQISQRNHGVDSGFYPLGSCTMKYNPKVNELAARFDGFTKIHPYQQEK
                     TVQGALKLMYDLGRMLSEITAMEEFTLQPAAGAHGELTGLMIMKAYHHNRNNEKRTKI
                     IVPDSAHGTNPASAAVAGFDVVEIKSNDKGLVDLEELKAAVGEDTAGFMLTNPNTLGL
                     FDENILEISKIVHDAGGLMYYDGANANAILGISRPGDMGFDVVHLNLHKTFSTPHGGG
                     GPGSGPVGVKKHLAEFLPVPVISKKEEEYYMDYERPNSIGKVKSFYGNFGVLVRAYAY
                     ILSMGPDGLKEVAENAVLNANYIANKLKDKYFLPIDTECMHECVFSADWQKEKNGVSA
                     LDIAKRLLDFGFHPPTIYFPLIVHEALMVEPTETESKETLDEFISVMRQIAEEAENTP
                     EMVKTAPHTTVISRLDEATAARKPILRYTRLT"
     gene            complement(4791..6128)
                     /gene="gcvPA"
                     /locus_tag="PKH42_05125"
     CDS             complement(4791..6128)
                     /gene="gcvPA"
                     /locus_tag="PKH42_05125"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOA19845.1"
                     /translation="MTRYIGNTTDDRQQMLNEIGYKDIDSLFRAVPDSVRLKEKLKIP
                     EALSEMELIKKIKSLSDKNFNSDEYVCFMGAGAYDHYIPSAIDQLIMRQEFYTAYTPY
                     QPEISQGTLQAIFEYQTMISELTGLPVANASMYDGATALAEASAMSCEATRRKEVLVA
                     SSVHPESRRTLNTYAKFRNITVKEISYENGQISLEELNTKINDNTAAVIIQNPNFFGI
                     IEDVEKIAEITHKNKSLLVLSADPISLSILKSPGEMGADIAVGEGQPLGNSLNFGGPY
                     LGFMAVTEKLMRKMPGRIVGQTTDKEGRRGFVLTLQTREQHIRREKATSNICSNQALN
                     ALQATMYLTFMGKEGLKKVAKLCLNKSHYAYSELIKTNKFKPLFTAPFFKEFAVISDK
                     PVSDLNEKLLKSNIIGGYNIEIDYPELKNAYLLAVTEKRTKEEIDALVRKAGE"
     gene            complement(6139..6513)
                     /gene="gcvH"
                     /locus_tag="PKH42_05130"
     CDS             complement(6139..6513)
                     /gene="gcvH"
                     /locus_tag="PKH42_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOA19846.1"
                     /translation="MKLLPELKYVESHEWVRVEENKAYVGITDYAQEQLGDIVYVEVP
                     EVGTVVEAGDQFVVLESVKAASDVYAPVSGTVVEVNEELEDNPALINESAYDAWIVAI
                     DMSMPTEVDSLLSAEEYEELCE"
     assembly_gap    6527..6626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6627..7721)
                     /gene="gcvT"
                     /locus_tag="PKH42_05135"
     CDS             complement(<6627..7721)
                     /gene="gcvT"
                     /locus_tag="PKH42_05135"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOA19847.1"
                     /translation="MKKTPLYEKHIQLKGKMIDFGGWSLPVEYTGIIPEHEAVRTKAG
                     IFDVSHMGEITVKGEDSEKYLQMLLTNDISLLNNNQIAYTAMCYPHGGVVDDLLVYKY
                     SNTDYLLVVNASNTEKDYQWMKENIFGNTEILNVSENYAQLALQGPLAETILQKLTSK
                     NLNEIEFYYFSDNVKVGNIEALVSRTGYTGEDGFELYFAYDKAEEMWDLILEAGKDEG
                     LIPAGLGARDTLRFEASLPLYGHELNENITPLEAGLGFFVKLNKENFIGKEALARQKS
                     EGLKRRIVGFEMIDRGIPRNNYEVYKEGEKIGYVTTGSFSPTLKKNIGLALIDSAYSK
                     EGTEIEILIRNKNSKAKVIKKPFYSKKYKKM"
     assembly_gap    8082..8181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8182..8405)
                     /locus_tag="PKH42_05140"
     CDS             complement(<8182..8405)
                     /locus_tag="PKH42_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19848.1"
                     /translation="MVIKKFSFSNKNNDEKLLNWEDSVKLDSQQKLREQMKQMNNENK
                     NRNKNDNRFDNRNDNKFDNRNENKFDNRND"
     gene            complement(8550..9488)
                     /locus_tag="PKH42_05145"
     CDS             complement(8550..9488)
                     /locus_tag="PKH42_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOA19849.1"
                     /translation="MDNFIFSLNVVLPLVFLMFIGSFLRRINIFDEDFLRKLNKITYC
                     VLLPVLLFNNIYTSKVSGEIDLEFIIFFVILALAVIGILLIVIPKYEKDNRNRGVLIQ
                     GLFRGQFVFFGIPLMANIFGDEGLVVLSTLIAIIVPIYNFLAVIILDLFTDERHNYKA
                     TIISILTNPLVIGSLLGIAASVLQIKLPSALNKTIADISKTVTPISLMILGGVIEINN
                     IWKNRKKLILIAAVKLFIIPSFAALITIFFGYRGIELGAVLSMIAPPTAVASYTLAQQ
                     HDCNDELAGQIVFFTTLISPFSIFLFIFVFKTFGLF"
     gene            complement(9501..10439)
                     /locus_tag="PKH42_05150"
     CDS             complement(9501..10439)
                     /locus_tag="PKH42_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOA19850.1"
                     /translation="MENLIFSLNAVLPLVFLMLLGAFLRKINVFDEDFLGKANKFIFK
                     VLFPFFLFNNIYSSKISEEIDSKFIIFVVIISIAVVGLLIVIIPKYEKDNKNRGVIIQ
                     GLYRGNTTLFATALISNIFGNEGLAIVTMLIAIISPTYIFLTVIILDLFTDERQNYKK
                     TLISILSNPLIIGSLLGIAVYTLQIKLPLAMEKTIADIAKTTTPIALMTLGGEIEINN
                     IWKNIKPLVFVAAGKLIIFPAIATLIAIFFGYRGVELGAILSMTAPPTAVTSYALAQQ
                     HNCNYELAGQIVFLTTLISPFTIFLFVYIFKAAGLF"
     gene            10738..12717
                     /locus_tag="PKH42_05155"
     CDS             10738..12717
                     /locus_tag="PKH42_05155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOA19851.1"
                     /translation="MENLKLKDFLDYTFLSGLELSPDKNYGAFVVHTSDFEDNKYLSN
                     IWLYNLTTSEYKKLTNMNEEKSFIWLDNNTILFSSLRDEKLKKRIQDGEHWTVFYAID
                     IQGGEAYEYMRIPMKVNNIKKIADEKFLLTAIYDHYGINLHSRKGEEKTKAIELIKEN
                     KDYEILDEIPFWSNGEGFINKKRNRLYLFDKGTKDIVPISNQYENIKVTSVKDGKALY
                     VSSIYTNKMELTTGLYMYDLYSSKALCLVEDLEYNIEFAEFVGDDIIVAATTMDKYGI
                     NENFNFYKLQDGELVLLSKHDFGLNNSVNSDCRYGEGAARRVFDNCLYFISTENKSSF
                     IKKLSLDGSIEKLTEDNGSVDCFDICSQGIIFIGLRGSRLQEIYTLQKIKETQRTKFN
                     EKIVEIKTIVKPESINFQNDGVTIEGFVLKPVNFDEKKCYPGILNIHGGPKTVYGEVF
                     YHEMQYWANEGYFVFFCNPRGSDGRGDEFADIRGKYGTIDYDDLMKFTDKVLEKYPQI
                     DCTRIGVTGGSYGGFMTNWIIGHTDRFKCAVSQRSISNWISKFGTTDIGYYFNTDQNL
                     SSPWDNVEKMWFHSPLKYANQAVTPTLFIHAEEDYRCWLAEGLQMFTALKYHGVDARL
                     CMFRGENHDLSRSGKPRHRVRRLEEITNWFEKYLR"
     gene            complement(12865..13074)
                     /locus_tag="PKH42_05160"
     CDS             complement(12865..13074)
                     /locus_tag="PKH42_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19852.1"
                     /translation="MFNNILGDSGDSLLFFFLLLVVLACFCGDDGLVGGIGGFGDNDS
                     LLFFFLLLVILFCSCDRVGGIGDRC"
     gene            complement(13137..13424)
                     /locus_tag="PKH42_05165"
     CDS             complement(13137..13424)
                     /locus_tag="PKH42_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19853.1"
                     /translation="MDKRKCDDNMIERMYDIIELIYILNRLTNKFCEDEHSKQYLFDI
                     MDNMQNVKYSYDKYRGKCSNRNLIKHECHNYCTKDSFIDKIIFYLKSLNEE"
     gene            13658..15028
                     /locus_tag="PKH42_05170"
     CDS             13658..15028
                     /locus_tag="PKH42_05170"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOA19854.1"
                     /translation="MRAFFNIVIIGLIGLLSSTYLFNLSPVDYKGTIEIETPVEESTL
                     SLEVEEDKILNPESITIKHTASKEEVIKIVSNSIFNVDIYKDNKLVKSNIDNLEPVSP
                     SIDATISVNKDNPIITAVNIAQKNLGLEDGVYKFVFNSNIIADIDKASVSVTVTYDTT
                     GNYYPAVNTAPAGTKGLTLYFPTKNADTLIPVTRFVVEDKSITRMAIEQLQNGPLSKE
                     LISVIKDVTNTTYNNGNVVIDLPSSYTAYNTGSTGAVLAYESFVKTIFAVDRYWPIHS
                     VTFTVDRKNVDTYFHGMSREAINYLPNIKRNYLLYLAHKADNRYYLFEYQLSPEEIGI
                     AENDSIEMKARKIFDAYSNTDIEYGISPVPKTVTLNNVSLQGRTLILDFNKDFLNVYE
                     DKNDLRHMMIESFLYTFTTIPGVDSIKITAENKEITDFIDVLDTTKILYPPEFINPEA
                     VEEKEE"
BASE COUNT         4591 a         2748 c         2342 g         5070 t
ORIGIN      
        1 aaatctactt catcagcttt tattgcttca aaaagtacgg ttgatgccaa atttgtctga
       61 agagtggtat ttaaatcagt attttcttca ataagaatac tgaacatttc agccataatg
      121 tattgttccg taaagttttt tccaccgact cttatttcca aatttttttc attgctgccg
      181 gtacaacctg ctgcagttgt caataacata ataattatta aaaatattgt tgttattttt
      241 attttgttct tcatcgaaat acctccttta atattatttc attcctcttg gagtaagctt
      301 atgttctaca tatccgagca acttgtctgc tacgacagct aacaaggctg caaggattgc
      361 tccaaccaat atcatatcat tacgatacat ttgaattcct ttaaaaatta attcccctaa
      421 tcctccggct ccaatatatg ctgcaatggt agccgagcca atatttatta cggtagcaat
      481 tctcacccct gccataatta cggctaatga ctggggcagt tcaaccatgc gcagtatttg
      541 aaaattggtc atacccattc ctctgcctgc atcaattaaa gatggcgata cttcattgat
      601 accggtatag gtgtttttta ttataggaag cagtgcataa aggaaaagca ccaaaatagc
      661 agtttctttg ccaataccca taaagggtat tagaatcccg aaaaatgcaa tgcttggaat
      721 ggtttgaaaa atattagcac ttgccaatat cgggtcagcc agtcgtttat aacgggttac
      781 tataatacct agcggtactc cgataacaat agctgtaatt attgaaattc ctgttatttg
      841 taaatgctca atcgttaatc gtattatatt gttcatatta ttttgtacat aatccaacag
      901 ttgcatattt atcactcctt tccatcaaac agctatgctc ttcctaatac attaactaag
      961 cttgacctgg taacaagccc caacagatgc ttttcatcat caattaccgg cacatatccg
     1021 acttgccctt gagccataat ggataatact tgattgacat ttgcatcttc atttacatga
     1081 ttaaattctg ttcttgttat ttcaataact gttcccccat taccaaagtt atcaagtata
     1141 tcattggatg tggctattcc gataaagttg ccaaattcat cgacaaccat caaactgtca
     1201 actttatgca ggcgcatctt ttccagtgcc tgggtaagag tacgctccgg tcggatggtt
     1261 acaggcttct ttatcataat atctttaact ttaatatatt cgggacgttt taacagcctg
     1321 tcttttccaa taaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtttt
     1441 gattcactcc agtctttaag atatggaacg agccccacat tttgagcaat attcatatga
     1501 ggaaaaagac cgatttgttg tattacatag cccatattcc tcctaagctc cacaggatta
     1561 attttgctga tatctgtatc attgatataa atacttcctt ccgtaggctc tattaaacga
     1621 ttaatcatac gcaaggtagt tgttttgcca caaccgctgg gtccgattaa tactacggtt
     1681 tcgccggatt taaaatttac agtcaagttg tcaacagcta cagaatcacc atattttttg
     1741 gttacatttt tgaaacttat caaaataaaa ccttccttct ccattattat gtttattgat
     1801 ttaaacctca aaatcaattt taaattgata taataaaaat aaagccatag agattcatag
     1861 ctttatcgaa taatcaaaaa gcttttatac tatcaatccc ccttcatacg cttacgaggt
     1921 tagctgacgg attcggactg aagagtagcc ctacttcata aagaagattc accccaaaaa
     1981 gttggttccc ccgctccatc cacatgttgg actcagcgtt gtataaaaag aaaaattatt
     2041 taattagttt tataattata atctaaaagt atattaaagt caagaaataa aaatcgacta
     2101 atttattcaa attcaatcgt tgcaggcggc ttagcagtga tatcatatac aactctgttt
     2161 acattctcaa cctcgttaac tattctgctg cttacttttt ccaagacttc atagggtatt
     2221 cttgcccact cggaagtcat gccgtttgta gaatgaactg ctcttagggc aattgtgtaa
     2281 caatatgttc tttcatctcc ggttacgcct acgcttttga tgtcgggaag ggctgcaaag
     2341 tattgccata tcttgttttg aagaccggct ttttttattt catctctgaa aataaagtct
     2401 gcttctctta ctatttttaa tttttcttcg gttacttctc ccaatacacg gattccaaga
     2461 cccggtcccg ggaatggctg tctttcaact atctcttcag gcagacccaa ctctcttcca
     2521 actagccgaa cttcatcttt atacaattgt ctcagaggct caataagttc aaagtcaaca
     2581 tcatcaggaa gccctcccac attgtgatgg cttttgatta ctgaagccgc agatgttccg
     2641 ctttctataa catccggata aatcgtgccc tgaacaagat agtcgatgtg tccgaatgtg
     2701 ttcttaagtt tctctttttc ttcttcaaac acccttataa actcttcgcc tattattttt
     2761 ctcttctttt caggctcagt tacacctgcc aattttctga ggaatctgtc ctgagcattc
     2821 acacgaatca gattcatgtc taatttattt ttaaatactt tttcaacctg gtcagcttcg
     2881 tcctttctta aaagtccgtg gtctacaaaa acacatatta aatttttacc tattgctttg
     2941 tgcaccattg ttgcagctac cgatgaatcg acacctcccg acaaggcaca aagagctttt
     3001 ttgtctccaa cttgtttttt tatttgtaaa attgaatctt caacaaaatt tctgacattc
     3061 cagtcttttc tcatttgagt acccctaatt tttaaagttt attaagcttt gccatcatgc
     3121 aaagtatctc agactttgag attcttcatt gtgttcagaa tgaaaagcta tacctgacat
     3181 caataagatt atcaaataaa atatatggtg tcaataaaaa ggannnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnngtttaca taattggtaa ttatgttaac ctcgtatatc
     3361 gaagtatggg ttttcttgca gctgttgctt catctaacct gcttataact gttgtgtggg
     3421 gagctgtttt taccatctcc ggagtatttt ctgcttcttc tgctatctgt ctcatgacag
     3481 aaataaattc atccagtgtt tcttttgact cggtttccgt gggttcaacc attaatgctt
     3541 catgaacaat taaggggaag taaatcgttg gaggatgaaa tccgaaatcc agcaatcttt
     3601 ttgctatatc gagagctgaa actccattct tctccttctg ccaatccgct gaaaacacac
     3661 attcatgcat gcattccgtg tcaattggca ggaagtattt gtccttaagc ttatttgcaa
     3721 tataatttgc atttaaaact gcattttcag ctacttcctt aagtccatcc ggacccatgc
     3781 ttaaaatata tgcatatgct cttaccaata caccaaaatt accgtaaaat gattttactt
     3841 tgccgattga atttggtctt tcataatcca tgtaatactc ttcttctttt ttagaaataa
     3901 ccggtaccgg cagaaattcc gcaagatgtt ttttcactcc tacaggacct gaacccggac
     3961 cgccgcctcc atgaggtgta ctgaatgttt tatggaggtt taagtgcacc acatcaaatc
     4021 ccatatctcc cggccttgat atgcctaaaa ttgcatttgc attggctccg tcataataca
     4081 tcaagccgcc ggcatcgtgg actattttag atatttccaa tatattttcg tcaaataaac
     4141 ctaatgtatt gggattagtc agcatgaatc ctgctgtgtc ttctccgact gcagctttca
     4201 attcttctaa atctaccaag cccttgtcat tagattttat ttcaactaca tcaaatccgg
     4261 caactgccgc agaagcggga tttgtaccgt gtgctgaatc cggaactatt attttggttc
     4321 ttttttcatt atttctgtta tgatgataag ctttcattat cattaatccc gtcagctctc
     4381 catgagctcc tgccgccggc tggagagtaa attcttccat agccgttatt tcagaaagca
     4441 ttctgcccag gtcatacatt aattttaatg caccctgtac tgttttttcc tgctgatacg
     4501 gatgaatttt tgtaaacccg tcaaatctag ccgccaattc gtttactttg ggattatatt
     4561 tcatggtaca gcttccgagc ggatagaatc cggaatctac accatgattt ctttgagaaa
     4621 tttgtgtaaa gtgacgaact gcatcaagct cgctgacttc aggaagctca ggagcctcag
     4681 ctcgtaagta ttttttatct atcacatcta aggatacatc cggcacatcg cattcaggaa
     4741 gtgaatatgc ctttctgcct tttttacttt tttcaaatat taattgcata ttactcacct
     4801 gcctttctta ctaatgcatc tatttcttct ttggttctct tttcggttac cgcaagcagg
     4861 taagcatttt ttaattcagg ataatcaatc tcaatattat agccgcctat tatattgctt
     4921 tttaagagtt tttcatttaa gtcgcttaca ggtttatcgc ttattacagc aaattcttta
     4981 aagaatggtg ctgtaaacaa gggcttaaat ttattggttt taattaactc gctataagca
     5041 tagtgggatt tgttaagaca aagctttgca acttttttta gtccttcctt tcccataaat
     5101 gttaaataca tggttgcctg cagtgcattt aaagcttggt tagagcatat atttgaggtt
     5161 gctttttctc ttcttatatg ctgttccctt gtttgaagag tcagtacgaa tcctctcctg
     5221 ccttctttat cggtcgtctg tcctacaatt cttccgggca tttttcgcat caacttttct
     5281 gtaacagcca tgaagcctaa ataaggtcct ccgaaattaa gtgaatttcc aagcggctgc
     5341 ccttcaccca cagcaatatc cgctcccatt tctcccggac tttttaaaat tgacaatgaa
     5401 ataggatctg cgcttaagac caaaagactt ttgtttttat gagttatttc ggcaattttt
     5461 tctacatctt caattatgcc gaaaaaattc gggttttgta taattacggc tgcagtatta
     5521 tcattaatct ttgtatttag ctcttctaag cttatttgac cgttttcata tgatatttct
     5581 tttaccgtta tatttctgaa tttagcatat gtatttaaag ttcgcctact ttcgggatgg
     5641 acagaagatg ctactaatac ttccttcctg cgtgttgctt cacaagacat tgcactagct
     5701 tccgccaaag ctgtcgcacc gtcatacatt gatgcattag caacgggaag tccggttaat
     5761 tcacttatca ttgtttggta ttcaaatatt gcctgcaatg ttccctgact tatttcaggc
     5821 tggtagggag tatatgctgt atagaattcc tgcctcatta ttaactgatc tattgctgaa
     5881 ggtatgtaat ggtcatatgc gccggcaccc ataaaacata catattcatc gctattaaaa
     5941 tttttatcgg ataatgattt tatttttttt atcagttcca tttctgacaa agcttcaggt
     6001 atttttaact tttcttttaa tcgtaccgaa tcaggaacag ctctgaacag agaatctata
     6061 tccttatatc cgatttcatt tagcatttgc tgcctgtcat cagtggtgtt tccaatatac
     6121 cttgtcataa ctgctccctt attcacataa ttcttcgtat tcttcagctg ataacaagct
     6181 gtcaacttca gtaggcatgc tcatatctat tgctacaatc catgcatcat atgcagattc
     6241 attgattaag gccggattgt cttccaactc ttcatttact tctacaactg ttcctgaaac
     6301 gggggcatat acatctgaag ccgccttaac tgattcaagt acaacaaact ggtcgcctgc
     6361 ttcaactaca gttccaacct caggcacttc cacataaaca atatccccta actgttcttg
     6421 tgcatagtca gtaattccta catatgcctt attttcttct actctcaccc attcatgaga
     6481 ttcaacgtat tttaattctg gtagtaattt cataatttcc tccgtgnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnncatc tttttatatt ttttggaata aaaaggcttt
     6661 ttaataactt ttgccttaga atttttattt cttattaaaa tttctatttc tgttccttcc
     6721 tttgaatatg cagaatctat gagggctaat cctatgtttt tctttaaggt aggtgaaaaa
     6781 cttcctgttg ttacatatcc tattttttcc ccttctttat atacttcata gttgtttctc
     6841 ggtattcctc tgtcaatcat ttcaaaaccg actattcttc ttttaagacc ttctgatttt
     6901 tgtctagcta aggcttcctt tcctatgaaa ttttctttgt tcagctttac aaaaaaacca
     6961 agtcctgctt ctaagggcgt aatattttca tttaattcgt ggccataaag gggcagtgat
     7021 gcttcaaatc ttaatgtatc tcttgcgcct aatcctgccg gtatcaagcc ttcgtctttt
     7081 cctgcttcta aaatcaaatc ccacatttct tcagccttat cataagcaaa atataactca
     7141 aaaccatctt ctcccgtata accggtcctg gaaaccaatg cttcaatatt gcctactttt
     7201 acattatcgc tgaaataata gaattctatt tcatttagat ttttacttgt caacttttgc
     7261 aaaattgttt cagccagagg tccttgcaga gcaagctggg cataattttc tgaaacattt
     7321 aatatttcag tatttccaaa tatgttttct ttcatccact gataatcctt ttcagtgttg
     7381 gatgcattaa caaccaatag ataatctgta ttgctgtatt tatagactaa caaatcgtct
     7441 actactccgc cgtgaggata acacattgcg gtataggcaa tttgattgtt attaagcaat
     7501 gaaatatcat tagttaataa catttgcaaa tatttttcgg aatcttctcc tttaactgtt
     7561 atttcaccca tgtgagatac atcaaaaatt cctgcttttg ttcttacagc ttcatgctcg
     7621 ggaattattc ctgtatattc aaccggcagg ctccacccgc caaaatcaat catctttccc
     7681 ttaagctgaa tatgtttttc atataaaggc gtttttttca ttacacactc ctcttaaaat
     7741 taaagacaga aaatatctgt ctttgttttt gatattattt tttattcatt atcactttat
     7801 taaaattatt tgtcaatatt ataaaaccaa taaatatccg tgtcaatata tattgtttgt
     7861 gaaaaaaaat acttttatat gcatattact ttcatctacc ggtattaaaa aacggtcatt
     7921 gagaatgacc gtttcaaata cccaactatt ttaaattatt gttctgactg tttttatttt
     7981 tgtttttatt gttgttttta ttatcctggt tgtttttgtt cagacttttc cagtcgtcat
     8041 catttctact gtcaaatttg ttgttgtcgt ttcgattgtc gnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nttgtcgttt cgattgtcga atttgttctc atttcgattg
     8221 tcgaatttgt tgtcgtttcg attgtcaaat ctgttgtcat ttttgtttct gtttttgttt
     8281 tcgttattca tttgtttcat ctgctccctt aatttttgtt ggctgtctaa tttgacactg
     8341 tcttcccaat ttaataactt ttcgtcatta ttcttattgc taaaactgaa tttctttatt
     8401 accatcatcc cttcatatgc atgtatttga agatgtttgt catctacatg aatattatta
     8461 gctttttcat attaattatt attccaataa atgcttaatg tggggttggt atccataaat
     8521 tcaatttttt aactgcaata ataaaattac taaaacaaac caaaagtttt aaaaacaaaa
     8581 ataaacaaga aaatactgaa ggggctgatc aatgttgtaa aaaatactat ttgtcctgcc
     8641 aattcatcat tgcaatcatg ctgctgtgcc aaagtatagc ttgccacagc cgtaggggga
     8701 gctatcatgc ttaatactgc tcctaattca attcctctgt atccgaagaa tatggtaatc
     8761 aatgctgcaa atgaagggat aataaataat tttactgctg ctataagaat taactttttt
     8821 ctgtttttcc atatattgtt tatttctatc actccgccga gaatcattaa tgatatgggt
     8881 gttaccgttt ttgagatatc agcaattgtt ttattaaggg cggagggaag ttttatttga
     8941 agaacagatg ctgcaattcc aagcaaggag ccgattacta aaggattggt taaaatgctt
     9001 attatggtag ctttataatt atgcctttca tccgtaaata aatccaaaat aataacagcc
     9061 aagaaattat aaattggcac tattattgcg ataagggttg atagaacaac caatccttca
     9121 tctccaaaaa tattggccat aagaggtatt ccaaaaaaca caaattgccc tctaaacaga
     9181 ccctgaatta aaactcctct gtttctgtta tctttttcgt acttggggat tactatcaat
     9241 aatattccaa taacagccaa agctaaaata acaaaaaaaa taataaattc taagtcaatt
     9301 tcccctgata ccttgcttgt atatatgttg ttaaaaagaa gaaccggtaa tagtacacaa
     9361 tatgtaattt tatttaattt ccttaaaaag tcctcatcaa atatattaat tcttcgaaga
     9421 aaggaaccta taaacatcaa aaaaaccaat ggtaaaacaa catttaaaga aaatataaaa
     9481 ttatccatct tttgacctct ttaaaataat cctgctgctt taaaaatgta tacaaacaag
     9541 aaaatagtga atgggctgat taatgtagtt aaaaatacta tttgtcctgc caattcataa
     9601 ttgcagttgt gctgctgtgc caaagcatag ctcgtgacag ccgtaggagg cgctgtcatg
     9661 cttaatattg cacctaattc cactcctctg tatccgaaga aaattgcaat caaggttgca
     9721 attgcaggaa aaataattag ttttcccgct gcaacaaaga ctaagggttt aatattcttc
     9781 catatattgt ttatctctat ttctccgcca agagtcatca atgctatggg cgttgtagtt
     9841 tttgcaatat cggcaattgt tttttccatt gccaaaggaa gttttatttg aagagtatat
     9901 actgcaatac caagcaggga gccgattatt aaaggatttg ataaaatgct tattaaagtt
     9961 ttcttataat tttgtctttc atcagtaaat aaatccaaaa ttataacagt caagaaaata
    10021 taagtgggtg atattattgc tatcagcatt gttacaatag ccaacccttc atttccaaaa
    10081 atattgctga ttagtgctgt tgcaaacaag gtggtattgc cgcgataaag gccttgaatt
    10141 ataactcctc tgttcttatt atctttttca tatttaggga ttattacaat caataatcca
    10201 acaacagcta tagatattat aacaacaaat atgataaatt ttgaatctat ttcttcagat
    10261 atcttactgg aatatatgtt gttgaaaaga aaaaacggga ataatacttt aaaaataaac
    10321 ttatttgctt ttcctaaaaa atcttcatca aaaacattga tttttcgaag gaaagcaccc
    10381 aaaagcatca aaaaaaccaa tggtaaaact gcatttaaag aaaatattaa attttccatt
    10441 ataagtcctt tcatattctg taataataat taatctatgt agtcatgctc ttgacattat
    10501 atatcaatca ttttcaattt ttgaatacag catgtcctat taatgttcat atacatgact
    10561 tttcagtcta ttataattat actctcaaaa caattctaaa acaatagttt taattgcata
    10621 actacttttt aaaatatata atatcattat aacaagcaat atccgtaaac agcttttaaa
    10681 gaaatgcatg ttcttgttgt cccggattat catgtaaaag tattatagga ggcagaaatg
    10741 gaaaatttaa aactaaagga ttttttggat tatacttttt tatccggact tgaactgtca
    10801 ccggataaaa attacggtgc ttttgtggta catacgtcag attttgagga taacaagtat
    10861 ttgtccaata tatggttgta taatttgacc acctcagaat ataaaaagct tacaaatatg
    10921 aatgaagaaa agagttttat ttggcttgac aataatacta ttttgttttc ttcattgaga
    10981 gatgaaaaat tgaaaaaaag aattcaggac ggcgagcatt ggactgtatt ctatgccatt
    11041 gatatccaag gcggggaagc ttatgaatat atgcgtattc ccatgaaggt aaacaatatt
    11101 aaaaaaattg ccgacgagaa atttctatta acagcaattt atgaccatta cggcattaac
    11161 cttcattctc gcaaaggaga agaaaagact aaggcaattg aattaataaa agaaaacaag
    11221 gactatgaaa tattggatga aataccgttt tggtcaaacg gcgaaggatt tataaacaag
    11281 aaaagaaacc gtctatatct gtttgacaaa ggaacaaagg atattgtacc aatttcaaat
    11341 caatatgaaa atataaaagt aacaagcgta aaagacggta aagctttata tgtttcttca
    11401 atttacacca ataaaatgga attgacaaca ggattataca tgtacgattt atatagcagt
    11461 aaagctcttt gccttgttga ggatttagaa tataatatag agtttgccga atttgtaggg
    11521 gatgacataa tagttgcagc tactaccatg gataaatacg ggataaatga aaacttcaat
    11581 ttttataagc tacaagatgg ggaattggta cttctcagca aacacgattt cggtttgaac
    11641 aattcggtta actcggactg cagatatgga gaaggagctg caagaagagt attcgataac
    11701 tgcctgtact tcatatccac agaaaataaa agttccttta taaagaagct ttcattggat
    11761 ggaagtattg aaaaattaac ggaagataat ggatcagtgg actgcttcga catatgcagc
    11821 caaggtatta tcttcattgg gctcaggggc agcaggctgc aggaaattta tacgttacaa
    11881 aaaataaaag aaactcaaag aactaaattt aatgaaaaga tagtggaaat aaagacgatt
    11941 gtaaaacctg aaagtattaa ttttcaaaac gatggagtta ccattgaagg atttgtgtta
    12001 aaacctgtta attttgatga aaagaagtgc tatccgggaa tcttaaacat acacggaggt
    12061 ccaaaaacag tatatggtga agttttctat catgaaatgc agtattgggc aaacgaagga
    12121 tattttgtat ttttctgtaa tccaaggggc agtgacggca ggggtgatga atttgcagat
    12181 ataagaggta aatacggtac cattgactat gatgacctta tgaaatttac agataaagtt
    12241 cttgaaaaat atccacagat agattgcaca agaattggtg taacaggcgg ctcctatgga
    12301 ggctttatga caaactggat tattggacac actgacagat ttaaatgtgc tgtttcacaa
    12361 aggagtattt caaactggat ttctaaattc ggcactacgg acatagggta ttatttcaat
    12421 acagatcaaa acttgtcttc tccttgggat aatgttgaaa aaatgtggtt tcattcaccg
    12481 cttaaatatg caaatcaagc tgttactccc acattattca ttcatgcgga ggaagattac
    12541 aggtgctggc tggctgaagg attacaaatg tttactgccc ttaagtatca cggtgtcgat
    12601 gcaaggcttt gcatgttcag gggagaaaat cacgatcttt caaggagcgg taaaccccgc
    12661 cacagggtga ggagattgga agaaataact aattggtttg aaaaatattt aagatgagtg
    12721 aaggatctca tgctaaaagc ttcccaatat aggaagcttt ttaatgtact atcaataacg
    12781 gggaccaagt gtcgccctta atttagggac attcctcatg cacaaaggga ctatacccca
    12841 atcagaggtg tgtccccata ccatttagca gcggtcaccg attccgccaa ctctatcgca
    12901 gctgcagaat agtattacca acaataagaa gaagaataat aagctgtcat tgtcgccaaa
    12961 gccgccgatt cctcctacca atccatcgtc tccgcagaag catgcaagaa ctactaaaag
    13021 caagaagaag aataataaac tatctccgct gtctcccaat atattgttaa acatatagaa
    13081 cctcctaaaa ataattttaa tatatattat tcgttagact gctaattgtt actttattac
    13141 tcttcattta atgattttag ataaaaaatt attttgtcga taaaactgtc tttcgtgcaa
    13201 taattgtggc attcatgttt aattaaattt ctattgctgc atttgcctct gtatttatca
    13261 tagctgtatt tgacattttg catattgtcc attatgtcaa ataagtattg tttcgaatgt
    13321 tcatcctcac aaaatttgtt tgtgagtctg tttaatatat atattaattc gattatgtcg
    13381 tacattcttt ctatcatatt atcgtcacat tttctcttgt ccactttcat cactccattc
    13441 attatattat atgaattttt tgaagtgctg atagtagaat tgaaacattt atttcattat
    13501 tcctttgatt ttatcaagtt ttattgtata ataagttaaa cagattattc acatacgttc
    13561 agaattatca cgtataatcc acacaaaaat aattgaatac attttgtcat cctgagtgta
    13621 ctgccccttt tacataaatt atatacgagg tactaatatg agagcatttt ttaatatagt
    13681 aataatagga cttataggtt tgttatcatc cacatatttg ttcaatttgt cacctgttga
    13741 ctataaagga acaattgaaa ttgaaactcc tgttgaagaa agtactttaa gtttagaagt
    13801 cgaagaagac aaaatactaa atcctgaatc aataacaata aagcatacag cctctaagga
    13861 agaggtaatt aaaattgtca gcaacagcat ttttaatgta gacatataca aggataataa
    13921 attggttaaa agtaatattg ataatttaga gccggtttct ccttcaattg atgcaaccat
    13981 ttcagttaat aaggataatc caatcattac tgccgtaaat atagctcaaa aaaacttagg
    14041 actggaagat ggagtatata aatttgtatt taattcaaat ataatagcag atatagataa
    14101 ggcttccgta tcagtaacag ttacctatga tacaaccggt aattattatc ctgcggttaa
    14161 tactgcccct gcgggtacta agggactcac cctgtacttc ccaacaaaaa atgcagatac
    14221 tttaattcct gttacgaggt ttgtagtaga ggataaatca attacaagaa tggcaattga
    14281 gcagctgcaa aacggtcctt taagcaaaga gcttatatca gtaatcaagg atgttacaaa
    14341 cacaacttac aataacggta atgtggtaat tgacctgcca agttcatata ctgcatataa
    14401 taccggcagc acgggagcag tcctggccta cgaatcattt gtaaaaacaa tatttgccgt
    14461 tgacagatat tggcctattc acagcgtcac atttactgtt gacagaaaaa atgtagacac
    14521 ttacttccat gggatgtcaa gagaggcaat aaattatctt cctaatatta agagaaatta
    14581 ccttctgtat ctggctcata aggcagacaa cagatattat ttatttgagt atcagctatc
    14641 cccagaggaa ataggtattg ctgaaaatga ttcaatagaa atgaaggcgc gaaaaatatt
    14701 tgatgcatat tcaaatacag acatcgaata cggcattagt ccggtaccta agactgtaac
    14761 gctaaacaat gtatctctgc agggaagaac acttattctt gattttaata aggatttttt
    14821 gaatgtttat gaggataaaa atgatttaag acatatgatg atagaatcct ttttgtatac
    14881 tttcaccaca ataccgggtg ttgacagcat aaaaattact gccgaaaata aagaaataac
    14941 tgattttatt gatgtgctgg atacaacaaa aatattatat cctccggaat ttattaatcc
    15001 ggaagcagta gaagagaaag aagagtaatc aaaaaaaaaa gtaagctatg tcattctgaa
    15061 gcctcagaat gacatgcagc ttattaattg caataacagg ctttgcctaa tggttattca
    15121 tacaatcgcc cttgtctttt taacaatgaa g
//
LOCUS       DAOPUE010000040        14729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4159_length_14729_cov_9.710849, whole genome shotgun sequence.
ACCESSION   DAOPUE010000040 DAOPUE010000000
VERSION     DAOPUE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14729
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_14729_cov_9.710849"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKH42_05175"
     CDS             complement(<1..1337)
                     /locus_tag="PKH42_05175"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOA19855.1"
                     /translation="MDTLMIAPIVGVLALLFAFYKASIVSKAEPGNDRMQEISSYIHE
                     GAMAFLAREYKSVSIFAIVMFFILGFAINWPTAIAFAAGASCSALAGYFGMTVATKAN
                     VRTTNAAMQSGMGKALDIAFSGGSVMGMTVVGLGILGTGIFWLIFEDAAVVTGFSLGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGAIVSAITLGVLAYQGKNGVSFALIVSAVGIIASILGTLFVKGDKNPQKA
                     LNMGTYASSAIVIAASFFLSKSILGSYQPFGSIIFGVFVGLIIAKITEIYTSDEYKYV
                     KEIASQSETGTATTIISGLSVGMRSTAFPIIVLAIGIIGAYAIAGYTSNGVDPVKGLF
                     GISLAAVGMLSTTGMTIAVDAYGPISDNAGGIAEMCELPHEVREITDRLDSVG"
     gene            complement(1467..1697)
                     /gene="secG"
                     /locus_tag="PKH42_05180"
     CDS             complement(1467..1697)
                     /gene="secG"
                     /locus_tag="PKH42_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOA19856.1"
                     /translation="MELVVRILLIIASLILIASILLQSGKSAGMSGEIAGGAESIWGK
                     SKGRSFEGKLEKATTISAIIFVIASLVLVAIQ"
     gene            complement(1870..3159)
                     /gene="eno"
                     /locus_tag="PKH42_05185"
     CDS             complement(1870..3159)
                     /gene="eno"
                     /locus_tag="PKH42_05185"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOA19857.1"
                     /translation="MSIITDIYAREILDSRANPTIEVEVWTESGGYGRAGVPSGASTG
                     AFEAVELRDGDKNRYLGKGVLKAVDNVNNIIAPELIGRDSLAQVEIDKLLIELDGTPN
                     KGKLGANAILGVSIACAKAAADTLGLPLYQYLGGVNGKVLPVPMMNILNGGQHADNNV
                     DIQEFMIMPVGAESFKEALRMGAEIFHNLKIVLKGRGLNTAVGDEGGFAPNLSSNEEA
                     LVTIVEAIKKAGYIPGKDVMLALDVAASELFDAETKMYNLEGEGRKFTQTEMVDYYTE
                     LVSKYPIISIEDGMDEEDWEGWKMLTEKLGDKVQLVGDDLFVTNTVRLKKGIELNVAN
                     SILIKLNQIGTITETLDAIEMAKRAGYTAVVSHRSGETDDSTIADLVLAVNAGQIKTG
                     APSRMDRVAKYNQLLRLEDILGDTAQYKGLEVFYNIK"
     gene            complement(3171..4706)
                     /gene="gpmI"
                     /locus_tag="PKH42_05190"
     CDS             complement(3171..4706)
                     /gene="gpmI"
                     /locus_tag="PKH42_05190"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOA19858.1"
                     /translation="MMNKVTALIILDGWGIGPDYEGNAIKRAKTPNFDVLLRRYPNTT
                     LSTSGYDVGLPKGQMGNSEVGHLNIGAGRIVYQDFTRITMSIENGEFEKNEALLSAIN
                     FAKKNNSTLHLIGLLSDGGVHSHNTHLYSLIQLAKKNGLIDVEIHCITDGRDVSPTSS
                     PNYIKELMKKTKQIGVGHIASIMGRYYGMDRNRQWDRVELAYNALINGEGEIYKDPLE
                     AIKSSYLKGVTDEFIKPLLIEKDNGELGTVKDNDAVIFFNFRPDRAREITRAFVDDDF
                     SYFDRKKINVKYVCMTMYDKTIENVEIAYKPNFVQNTLGEYLSRLGIKQLRVAETEKY
                     AHVTYFFNGEIEEPFENEVRILIPSPDVPTYDLKPEMSAFEVKNTVMEQLEKDMYKVM
                     IINFANPDMVGHTGDIEAAIKAVEAVDKCFGEIVNYIIRAEGTAIITSDHGNCEEMFD
                     RETLDIITSHTGNRVPFIVVDNKNFKLREGILADVAPTILELMGLEKPAEMTGKSLIV
                     HNE"
     gene            complement(4720..5460)
                     /gene="tpiA"
                     /locus_tag="PKH42_05195"
     CDS             complement(4720..5460)
                     /gene="tpiA"
                     /locus_tag="PKH42_05195"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOA19859.1"
                     /translation="MRKKLIAGNWKMNKDVEESIKLVNILKSSQLNKDVDVLVCPPFT
                     SLYPVIHELKNSSIKVGAQNMHFENSGAFTGEVSPPMLKNIGVEYVIIGHSERRQHFG
                     ETDQMVNKKVKAALNNGLKPILCVGESLEQREAGVEKETVRSQLIKDLDGITEGLNIV
                     VAYEPIWAIGTGRNATSAQANEMSSFIRSVLGDVFTADIADNIVIQYGGSVKASNAKE
                     ILEQSDIDGALVGGASLMADEFIKIINF"
     gene            complement(5489..6682)
                     /locus_tag="PKH42_05200"
     CDS             complement(5489..6682)
                     /locus_tag="PKH42_05200"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOA19860.1"
                     /translation="MNKKTLKDINVKNKKILVRCDFNVPLDKEGNITDDIRIKASLPT
                     INYLLDNGAAVILLSHLGRPDGEANKKYSLQPVAQRLSSLLGRGVCFEDCDVVVDDRV
                     KTNAAKLKPGDIMLLQNTRFRKEETKNAGTFAKELASLGELFVNDAFGTAHRAHSSNV
                     GICEYLHSALGFLVEKEVEIMGNALKTPKRPLTAILGGAKVSDKIAVIENLLNIADNV
                     LIGGGMMYTFLKAKGYNTGNSLLEEDKVELAKNIIEKASEKVVKLILPVDTVVAKEFK
                     NDTEFFTTDVDKIPDEYIGLDIGEKTVEIYKEIIGNSKTIIWNGPLGVFEMDNFANGT
                     NAVAKAIADNEDAISIIGGGDSAAAVEKTGLSENITHISTGGGASLEFLEGKMLPGIE
                     AIDNK"
     gene            complement(6757..7764)
                     /gene="gap"
                     /locus_tag="PKH42_05205"
     CDS             complement(6757..7764)
                     /gene="gap"
                     /locus_tag="PKH42_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOA19861.1"
                     /translation="MVRVAINGFGRIGRLALRLMAQDEDFKVVAINTNSDAETLTYLL
                     KYDTAHGNFMTDKISYKNNLMYVDGKEIITSRVKEPENCNWKELDIDLVIDCTGKFND
                     KEKAEAHIKAGAKKVLLSAPGKGDLKTVVYNVNHNILDGSETVVSAASCTTNCLAPLA
                     KVLNDEFGIVKGLMTTVHAYTNDQNIHDNSHKKGIMARRGRAAAANIVPASTGAAKTV
                     GVVLPELKGRLDGIALRVPTLTGSVVDLTVELGKNVTVEEVNQAVKNAANETIAYTED
                     PIVSSDVIGSRYACVFDALSTQIIEADGKQLVKLIAWYDNEMSFTAQLVRVAKYWGQM
                     R"
     gene            complement(7928..9325)
                     /gene="rpoN"
                     /locus_tag="PKH42_05210"
     CDS             complement(7928..9325)
                     /gene="rpoN"
                     /locus_tag="PKH42_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOA19862.1"
                     /translation="MKMGFELNLSQTQKLIMTPELRQAIQILQFNNVELTDFINKQLE
                     VNPFLEAVDNKSDVSSNEEHMNKNIENEEIDWRELAEKYDDLSYKSYERNIDNENKQS
                     FESYTSKKLSLKDHLMIQLGITVKTNKDKRIGEFIIESLDNKGYLGCSIQDISLLINE
                     DVVDVERVLRLIQTFDPVGVASRNLSECLMIQLKEKGIQDKNAYIIAEKYLEDIATNK
                     IQKIAKELHISVNRVQNICDIIKMLEPKPSRGFIVDSDNIRYIVPDATIEKLNDDYII
                     IINDSYLPTLTINNYYKNMVNELDDKEASRFLADKLNSSMWLIKSIEQRRITLYKVIE
                     SIIKFQRKFFDEGKMALRPLVLKDVADDIGMHESTISRATNGKYVQTPRGLYELKYFF
                     ASSLSEADGSGVSSTSVKSFIRKLIDEENPQKPLSDQKITERLNEQGINISRRTVAKY
                     RDEMRIPSSSMRRRY"
     gene            complement(9490..10665)
                     /gene="grdD"
                     /locus_tag="PKH42_05215"
     CDS             complement(9490..10665)
                     /gene="grdD"
                     /locus_tag="PKH42_05215"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HOA19863.1"
                     /translation="MSEKLVKQTIGNIFNQLADTLISGEYGQKPKVAVTALGSEHGED
                     EVYKAGINSLKFGIDTTVIGSKPNESVNTEVVNSEDDAHKKMEELLDKGSVDAAVTMH
                     YPFPIGVSTVGRIITPGKGKEMFIATTTGTSSTDKVEGMVKNAVYGIITAKACGIKNP
                     TVGILNVDGARQTEIALNKLKNKGYEINFAESKRSDGGAVMRGNDLLMGSCDVMVMDS
                     LTGNLMIKIFSSYTTGGSYESLGYGYGPGIGENFNKLILIISRASGAPLIEGAIRFAG
                     ELIKGNCLMIAKEEFNNAKKAGLDEILSEIKASKKSTAEEAEIVAPVKEVVTSQISGI
                     EIMDLEDAVKLLWKNGIYAESGMGCTGPIILVNEAKTNDALKILAANDFIAKEQIDC"
     gene            complement(10670..12208)
                     /gene="grdC"
                     /locus_tag="PKH42_05220"
     CDS             complement(10670..12208)
                     /gene="grdC"
                     /locus_tag="PKH42_05220"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HOA19864.1"
                     /translation="MNSVILGSSYIMVFAPDMVINNGTTQTTERTVNPNSEYLKEIRN
                     HLRTYEQVVSYLPNQTYIGNITPDELSKYEMPWFDKPLEGATREGKFGEIMPQDEFIV
                     LMQICDVFDLVKLEKQFVSAVKAKLMNHPLIDDSLIGRIKEGEEFDTIKKYVEAEHAE
                     PIYNNGALVGCVKRAHDIDINLSGHVMFENLVSKASNVLALLHLISKNNINKEEIEYI
                     IECSEEACGDMNQRGGGNFAKSCAEVAGLVNATGCDTRGFCAAPTHAIVEAAALVKAG
                     VFKKVIVSAGGSTAKLGMNGKDHIKKGYPILEDTIGGFAVLIGENDGVNPEINLDLVG
                     RHTVGTGSSPQAVITSLVSSLDKAGMKITDVDKYSVEMQNPEVTKPAGAGDVPLANYK
                     MIGALAVKRGDIDRKELENFTAKHGMTGWAPTQGHIPSGVPYLGFCREDILSGKIKNA
                     MIVGKGSLFLGRMTNLFDGVSFIIEANKGGQKETSAISEEQIKNLIAKALKDFASNLL
                     NEQE"
     gene            complement(12358..12831)
                     /gene="grdA"
                     /locus_tag="PKH42_05225"
     CDS             complement(12358..12831)
                     /gene="grdA"
                     /locus_tag="PKH42_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_145084612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12700..12702),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOA19865.1"
                     /translation="MSLLENKKIIIIGDRDGIPGQAIEECVKTAGGEIVFSSTECFVU
                     TSAGAMDLENQKRVKEFAEKYGPENIVVILGAAEGEAAGLAAETVTNGDPTFAGPLTG
                     VQLGLSVFHICEPEIKELVDESVYDEQVGMMEMVLDVDDIISEMTAIREEFSKYL"
     gene            complement(12859..13176)
                     /locus_tag="PKH42_05230"
     CDS             complement(12859..13176)
                     /locus_tag="PKH42_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOA19866.1"
                     /translation="MLVLDKTNFETEVLQGEGYIFVDFFGDGCAPCEALMPHVEKLAE
                     KYGDKLKFTKLNTTKARRVAIGQKVLGLPVIAIYKDGEKIEELVKDDATPENIEAMIQ
                     KYI"
     gene            complement(13187..14122)
                     /gene="trxB"
                     /locus_tag="PKH42_05235"
     CDS             complement(13187..14122)
                     /gene="trxB"
                     /locus_tag="PKH42_05235"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOA19867.1"
                     /translation="MSKVYDVLIIGAGPAGLSAGIYAGRARLSTLIIEKEKDGGQIVL
                     TSEVENYPGCLEGESGPTLVDRMAEQAKHFGVEKVYDEIVEVKLEGKEKILIGKKGEY
                     KGKAVIIASGATSRPIGCPGEKTYTGKGVSYCATCDAAFFEDLEVFVVGGGDTAVEEA
                     LYLTKFARKVSIIHRRNELRAAKSIREKAEANPKIHFIWDSVVKEIKGDGIVNSIVLE
                     NVKTKELTEIVADEDDMTFGIFVFIGYEPKTKIFEGILDIENGYLVTDDNMKTNIEGV
                     FAAGDVRVKSLRQVVTAAADGAIAAVQAEKYIETL"
     gene            complement(14445..>14729)
                     /locus_tag="PKH42_05240"
     CDS             complement(14445..>14729)
                     /locus_tag="PKH42_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14706..14708),aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HOA19868.1"
                     /translation="AVILTSTUGTCTRCGATMVKEIERAGIPVVHMCTIVPISLTVGA
                     NRIVPTIAIPHPLGNPSLSIEEEKEIRRKLLTRALKALETEVTEQTVFEK"
BASE COUNT         4064 a         3220 c         2118 g         5327 t
ORIGIN      
        1 ttgcctacag agtctaatct atccgttatt tctcttactt catgaggaag ctcgcacatt
       61 tcggcaatac ctcctgcatt gtcggatatt ggtccatagg catcaactgc tattgtcatg
      121 cctgttgttg ataacatacc tactgctgcc aatgatatac caaataatcc tttgacagga
      181 tcaacaccgt ttgaagtata acctgcaatt gcatatgctc ctataatacc tattgccaaa
      241 acaattattg gaaacgctgt ggatctcatt ccgacagaca aaccgcttat aattgttgtt
      301 gctgtacctg tttcggattg tgaagctatt tcttttacat atttatattc atctgaagta
      361 tatatttcag ttatttttgc tataattaat cctacaaata caccgaatat aattgaacca
      421 aagggttggt aactgcccaa tatagacttg cttaagaaaa atgatgcagc tattactatt
      481 gcagaagaag catatgtacc catatttaaa gctttttgag ggtttttgtc tccttttaca
      541 aacaatgtac cgagtattga cgcaataata cctacagcgg atacaattaa agcaaatgaa
      601 acgccatttt taccttggta tgccagaaca cccagtgtta ttgcagaaac tattgctcca
      661 acatatgatt caaataagtc tgcacccatt cctgctacgt cacctacatt atcgccaaca
      721 ttatccgcta taacagcagg gtttctcggg tcatcttcgg gaattcctgc ttcaacctta
      781 ccaaccaggt ctgctccaac gtctgcagct tttgtataaa tacctccgcc cacacgtgca
      841 aataatgcta ttgatgaagc gcctaggctg aatcccgtaa ctacagctgc atcttcaaaa
      901 attaaccaaa atatgcctgt tcctaatatt ccaagaccaa caactgtcat tcccatcact
      961 gagccgcctg aaaatgcaat atccaaggct ttgcccattc ctgattgcat tgctgcattt
     1021 gttgttctta cattggcttt tgttgctact gtcataccga aatatcccgc aagtgccgag
     1081 catgaagccc ctgccgcaaa tgcaattgca gtaggccaat taattgcaaa tcctaaaata
     1141 aagaacatta ctattgcaaa aatactaact gacttatatt ctcttgcaag aaatgccatt
     1201 gctccttcgt ggatatatga agatatttct tgcattctat cattaccggg ctcagccttg
     1261 cttacaattg atgctttgta gaatgcaaat aataaagcca aaactcccac tatgggagct
     1321 atcattaaag tatccatttg tttctttcct ccatagtatt tacaatttgg cagattaaat
     1381 aaaatctgct cattgatatt taataaattc taactcaata tcaacattag aatacttact
     1441 cattctttcc aattaattac aaaatattat tgaattgcaa ccaagactaa tgatgcaata
     1501 acaaatataa ttgctgatat agttgttgct ttttccagtt ttccttcaaa acttctgcct
     1561 ttgctctttc cccatataga ttcggctcca cctgcaattt ctcctgacat tcctgccgat
     1621 ttgcctgatt gaagcaaaat acttgcaatt aaaattaagc tagcaattat taaaagtatt
     1681 cttacaacta attccactaa aacacctcct gttcctttct tcgcataaca ttagaattat
     1741 aacaccaaag atttatgaaa tcaaccatat ttttttagat gagccatttc tcaggcctga
     1801 tttggggaca tgtctcctac cggcagaggc tatcccataa aaagggagcg tgtccccata
     1861 cctctaacat tattttatat tatagaaaac ttccaagccc ttatattgag cagtatcgcc
     1921 taagatgtct tccaacctga gcagttggtt atatttagca acacggtcca tccttgaagg
     1981 agctcctgtt ttaatctgtc ctgcattaac tgcaagaacc aaatcagcga tagttgaatc
     2041 gtcggtttct cccgagcggt gtgaaacaac cgcagtataa cctgctcttt ttgccatttc
     2101 aatagcatct agtgtttctg ttattgtgcc tatttgattt aattttatta atatagaatt
     2161 agcaacattt aattcgatac ccttttttag tcttacagtg ttggttacga ataaatcatc
     2221 accaactaac tgaactttat cacccagttt ttcggtaagc atcttccaac cttcccagtc
     2281 ttcttcatcc ataccgtctt ctatagatat tatcggatat ttgctcacca attctgtata
     2341 ataatcaacc atttcagtct gagtaaactt tctgccttca ccttccaagt tatacatttt
     2401 tgtttctgca tcgaataatt ccgaagcagc cacatcaaga gccagcataa catctttccc
     2461 gggaatgtag cctgcctttt taatagcttc aactattgta actaatgctt cttcatttga
     2521 tgataaattc ggtgcaaatc ctccttcatc accaacggcc gtatttaatc cccttccctt
     2581 taaaacgatt tttaaattgt ggaatatttc agctcccatt cttaatgctt ctttgaaact
     2641 ttcagctcct acaggcataa tcatgaattc ctgaatatcc acattgttgt cggcatgctg
     2701 cccgccgttt aaaatattca tcatgggtac aggaagaact ttgccgttta ctcccccaag
     2761 atattgataa aggggtaacc ccaatgtgtc tgctgctgct tttgcgcatg cgatggatac
     2821 tcccaatatt gcattcgcgc ccaattttcc tttgttggga gtgccgtcaa gttcaattaa
     2881 aagtttatcg atttcaactt gtgccaatga atctcttcct attaattccg gtgcaataat
     2941 attgttaaca ttatcgacag cttttaacac gcctttgcct aaatatctgt ttttatcgcc
     3001 atctctaagt tctacagctt caaatgctcc tgttgaagct ccggatggta cgcctgccct
     3061 tccgtacccg ccgctttctg tccatacttc tacttcaatt gtaggattcg ctctggaatc
     3121 caatatttct cttgcataaa tatctgttat tattgacata ctattctcct ttattcatta
     3181 tgaactatca aactttttcc tgtcatttct gcaggttttt cgagtcccat aagctcaaga
     3241 atcgtagggg ctacatctgc aagaattcct tctcttaatt tgaaattttt gttgtcaaca
     3301 actataaagg gcactctgtt gccggtgtga gaagtgatta tatcaagggt ttctctgtca
     3361 aacatttctt cgcagtttcc atggtctgat gtaattattg ctgttccttc cgcccttatt
     3421 atatagttta ctatttcacc gaaacattta tccactgctt ccactgcctt tatagctgcc
     3481 tctatatctc ccgtgtgtcc caccatatca ggatttgcaa aattaattat cataacctta
     3541 tacatatcct tttctaattg ctccataaca gtgtttttta cctcaaaagc actcatttcg
     3601 ggctttaagt cataagtggg cacatcaggt gaaggtatta aaattcttac ctcattttca
     3661 aaaggttctt ctatttcacc gttaaaaaaa tatgtaacat gagcatattt ttccgtttct
     3721 gcaactctta actgcttaat gcccagtctg cttaaatatt cacctaaggt attttgcaca
     3781 aaattaggct tgtaagcaat ttccacattt tcaatagtct tatcatacat tgtcatacat
     3841 acgtatttca cattaatctt ttttctgtca aaatatgaaa agtcatcatc aacaaatgcc
     3901 ctggttattt ctcttgccct gtcaggtctg aaattaaaga aaataaccgc atcgttatct
     3961 ttgacagtac caagctctcc gttgtctttt tcaatcaata ggggttttat aaactcgtct
     4021 gtaactccct ttaaatatga gctctttatt gcttcaagag ggtctttgta aatttcaccc
     4081 tcaccattta ttaaagcatt gtatgccaat tctactctgt cccactgtct gtttctatcc
     4141 atgccgtagt atcttcccat aattgaggca atgtgtccta cgccaatctg ttttgttttt
     4201 ttcatcaatt cttttatgta atttgggctg cttgtagggc ttacatctcg accgtcagtg
     4261 atgcaatgaa tttcaacatc gattaagccg tttttcttcg cgagttgaat taaactatac
     4321 aaatgagtat tgtgactatg tactccccca tcagataaaa gccctattaa atgcagagtt
     4381 gaattattct tctttgcaaa atttatggca ctaagcaatg cttcattttt ttcaaactca
     4441 ccattctcaa tagacatagt aattctggtg aaatcctgat acacaattct gcctgcgcct
     4501 atgtttaaat gtcctacctc tgaatttccc atttgtcctt tgggaagacc tacatcataa
     4561 ccacttgtag ataaagttgt atttggatac cttctcaata atacatcaaa attgggagtt
     4621 tttgctcttt ttatagcatt cccctcataa tcaggtccaa taccccatcc atcaagaata
     4681 atcaatgcag ttactttatt cataattcaa cctttctact taaaaattaa taattttgat
     4741 aaattcatca gccattaagc tggctcctcc aaccaatgct ccgtcaatat cggactgctc
     4801 caatatttct tttgcattgg aagcttttac actgcctcca tattgtatca ctatattatc
     4861 agcaatatct gcagtaaaaa catctcctaa tactgacctt ataaatgatg acatttcatt
     4921 agcctgagcg cttgtagcat tcctgcctgt acctatagcc catataggct catatgctac
     4981 aactatattt aaaccttccg ttataccatc taaatcttta attaactgac ttctaacagt
     5041 ttccttttca actccggctt ctctttgctc caatgattcc ccgacacaaa gaataggttt
     5101 aagaccgtta tttaaggcag ctttgacttt tttgtttacc atttggtctg tttcgccaaa
     5161 gtgttgtctt ctttcggagt gcccgattat tacgtattca acaccaatat tttttaacat
     5221 tggcggtgaa acttcacctg taaaagcacc gctgttttca aagtgcatat tttgtgctcc
     5281 tacttttatt gaagaatttt taagttcatg aattacagga tataatgatg taaagggcgg
     5341 acaaaccaac acatcaacat ctttattcaa ttgagaagac tttaatatat taacaagttt
     5401 tatactttcc tccacatcct tattcatttt ccagttgcct gcaattaatt tttttctcat
     5461 tttacctcct ccgcatgcag gggcattctt atttattatc tattgcttcg atgccgggta
     5521 acatttttcc ttccaagaat tccagcgaag ctcctcctcc cgtagaaata tgggttatat
     5581 tttctgacag tccggtcttt tcaacagctg ctgcactgtc tcctccgcct attatggata
     5641 ttgcatcttc gttatctgca attgcttttg ctactgcatt ggtcccgttg gcaaagttgt
     5701 ccatttcaaa aacacctaag ggaccgttcc aaattattgt ttttgaattt cctattattt
     5761 ctttatatat ctcaacagtc ttttcaccga tatcaagtcc tatgtattca tcaggaattt
     5821 tatcaacatc tgttgtgaag aattccgtat catttttaaa ttcttttgct actactgtat
     5881 caaccggtaa tattaatttt actacttttt ctgatgcttt ctcaattata ttcttggcaa
     5941 gttcaacttt atcttcttcc aaaagagagt ttcccgtatt atatcctttt gccttaagga
     6001 aagtatacat catacctccg ccaattaaaa cattatcagc aatattcaat aaattttcaa
     6061 ttactgctat tttatccgaa acctttgctc cgcctaagat ggcagtaaga ggtctttttg
     6121 gagtttttaa tgcatttccc attatctcaa cttctttttc taccaaaaac ccaagtgcag
     6181 aatgaagata ttcacagatg cccacatttg aggaatgtgc tctgtgagca gtgccgaatg
     6241 catcattaac gaacagttct cccagtgatg ccagctcttt tgcaaatgta cctgcattct
     6301 tcgtttcttc ttttctaaac ctagtattct gcaaaagcat tatgtctcca ggtttaagct
     6361 ttgctgcatt tgtttttact ctatcatcta ctacgacatc acagtcttca aaacaaacac
     6421 ctcttcctaa gagtgagctt aatctttgtg caacaggctg cagagaatat ttcttatttg
     6481 cttctccatc aggtctaccc aaatgggaca ataatattac tgccgcacca ttatccaaaa
     6541 ggtaatttat tgttggaaga gatgctttaa ttcttatatc atcagttatg ttcccctctt
     6601 tgtcaagggg aacattaaaa tcacatctta ctaatatttt tttattttta acgtttatat
     6661 cctttaaagt ttttttattc atgataaacc tccgttaacg tttatatgtc attaagggtt
     6721 gaccatatct ctacgcggtc gaagtctttg gttaaatcat ctcatttgtc cccaatactt
     6781 agctactctc acaagttgcg ctgtaaatga catttcattg tcataccagg caattaattt
     6841 aaccagctgt ttaccgtcag cttctattat ttgagtggac aatgcatcaa atacacatgc
     6901 atatctgctt ccaataacat cgcttgatac aatcgggtct tcagtatatg ctattgtttc
     6961 attggctgca tttttaacag cttgatttac ttcttcaaca gtaacatttt ttccaagttc
     7021 aacggttaaa tctacaactg aaccggtaag agtgggaaca cgaagagcta taccgtcaag
     7081 cctgcccttt aattcaggca aaactactcc aacggtctta gcagctcctg tagatgcagg
     7141 aacgatattt gctgctgctg cacgtcctct ccttgccatt ataccttttt tatgcgagtt
     7201 atcatgaata ttttggtcat ttgtataagc gtgaacagtg gtcataagtc ctttaactat
     7261 tccaaattca tcatttaata cttttgcaag cggagctaaa cagtttgttg tgcatgatgc
     7321 tgcagataca accgtttcac ttccatctaa tatattgtga tttacattgt atacgactgt
     7381 ttttaaatcg cctttgcccg gcgctgaaag caataccttt tttgctcctg ccttaatatg
     7441 agcttctgct ttttccttgt cgttgaattt tccggtgcag tcaattacca aatcaatgtc
     7501 taattctttc caattgcagt tttcgggctc ttttactctt gatgttatta tttctttgcc
     7561 atcaacatac attaaattat ttttgtagga aattttatcg gtcataaaat taccgtgcgc
     7621 agtatcatac tttaataaat aagtcaatgt ttcagcatct gaatttgtgt tgattgctac
     7681 aaccttgaaa tcttcatcct gagccataag ccttaatgct aatcttccaa tcctgccaaa
     7741 tccgttaatt gctactctta ccatttaatc ctcctataga aataacattt attataattc
     7801 taaatcctta ttaataaatc cgttgcaata aggataatat gtaacaaaca gattatatca
     7861 tactaaatat ataatgtgta gcatattata ataaaaaaat atgcatatct ttttgcatat
     7921 ttttctatta atatcttctc ctcatggaag atgacggtat tctcatttca tctctatatt
     7981 ttgctacggt ccttcttgat atatttattc cctgttcgtt taatctttcg gttatttttt
     8041 ggtcgcttaa cggtttttgc gggttttctt catctattaa ttttcttata aaagatttaa
     8101 cgcttgtgga tgacactccg ctgccatcag cttcacttaa gctgcttgcg aaaaaatatt
     8161 tcagttcata aagacctctt ggcgtttgta catatttacc gttagtagct ctgcttattg
     8221 ttgattcatg catgcctata tcatctgcaa catctttcaa aactaagggc cttaatgcca
     8281 tcttaccttc atcaaaaaac ttcctttgaa attttattat tgattcaatt actttgtaca
     8341 aagtaatcct tctttgctca atacttttta tcagccacat tgatgaattt aatttgtcag
     8401 ctaaaaatct gcttgcctct ttatcgtcta attcatttac catgtttttg taataattgt
     8461 ttatggtaag agtgggcagg tagctgtcat taattataat tatataatca tcgttgagtt
     8521 tttctatagt tgcatcaggt acaatgtacc ttatattatc actgtctact atgaatcctc
     8581 ttgacggttt gggctccagc atttttatta tatcgcaaat attttgcacc ctgttaactg
     8641 atatatggag ttctttagct atcttttgaa ttttgtttgt tgcaatatct tcaagatatt
     8701 tttctgcaat aatataagca ttcttatcct ggattccttt ttccttaagc tgtatcatca
     8761 agcattcact taagtttctg gatgcaactc ctacagggtc aaaagtttgt atgagcctta
     8821 aaactctttc aacatcaaca acatcctcat ttattaataa actgatatcc tgaatggaac
     8881 atcccaaata tcctttatta tccaatgatt caataatgaa ttcgcctata cgcttatctt
     8941 tgtttgtttt gacagtgata cctaactgaa tcatcaagtg gtctttcagt gaaagttttt
     9001 tggaagtata gctttcaaat gactgtttat tctcattatc tatatttctt tcatatgatt
     9061 tataacttaa atcatcatac ttttcagcta attctctcca gtcgatttct tcattttcta
     9121 tatttttatt catatgttct tcatttgaag agacatcact tttgttatcg acagcttcca
     9181 aaaaaggatt gacttctaac tgcttattga taaaatcagt taattctaca ttattaaatt
     9241 gcaagatttg aatggcttgc cttaattccg gtgtcattat aagcttttga gtttgggaaa
     9301 gatttaattc aaatcccatt ttcatggtat cactcctcac aattgtatta tatcaaaaat
     9361 tcataaattt tcaatataat tataaagcaa taaaaattca atctaataaa cataccaaca
     9421 taaaaaattc ttcatccttt gatgaagaat ttttgtgggg tttgcaccta aaatagggtt
     9481 agggcatctt taacagtcta tctgttcttt agctatgaaa tcattagctg caagtatttt
     9541 tagagcatca tttgttttag cttcatttac caaaattatc ggtcctgtgc atcccattcc
     9601 gctttcagca taaataccgt ttttccacaa caatttaacc gcatcttcaa ggtccataat
     9661 ttcaattccg gatatttggg aagttacaac ttcttttaca ggagcaacaa tttctgcttc
     9721 ttccgcggtt gactttttag atgcttttat ttctgataat atttcgtcca aacctgcttt
     9781 tttggcattg ttgaattctt cttttgctat cataaggcag ttgcctttta tcagttcacc
     9841 tgcaaacctt atggctcctt caattaatgg agcaccggaa gccctggaga taataagtat
     9901 caatttattg aagttttctc caataccggg accatatcca taacccaagg attcataact
     9961 tcctcccgta gtataggaag aaaatatttt aatcatcaga ttgcctgtaa gagaatccat
    10021 aaccataaca tcacaagaac ccataagcaa gtcattgcct cgcataacag ctcccccgtc
    10081 tgatcttttg gattcagcaa agtttatttc atagccctta tttttaagct tgtttagtgc
    10141 tatttccgtt tgtctggcac catctacgtt taaaattcca accgtcgggt tttttattcc
    10201 gcaagcttta gctgtaatta ttccatagac agcattctta accattcctt caaccttgtc
    10261 tgttgaagaa gttccggttg tcgtagcaat aaacatttct tttcctttac cgggagttat
    10321 gattcttcca acggtggata ctcctattgg aaacggataa tgcattgtaa cggctgcatc
    10381 aacactgccc ttatcaagca attcttccat ttttttatga gcatcgtctt ccgaatttac
    10441 aacttctgta ttgacacttt cattaggctt tgagccgatt actgttgtat caattccaaa
    10501 ctttaatgaa ttaataccag ctttgtaaac ttcatcttct ccatgctcac ttcccaatgc
    10561 ggtaaccgca acctttggct tttgaccata ctcaccgctt attaaagtat cggctaattg
    10621 gttaaaaata ttacctattg tttgttttac taatttttca gacatatctt cactcctgtt
    10681 cgtttaacag atttgaagca aaatccttta atgccttggc aattaaattc tttatctgtt
    10741 cctctgaaat tgcggacgtt tctttctgac ctcctttatt tgcttcaata ataaaggata
    10801 caccgtcaaa taaattagtc attcttccaa ggaataagct tcccttacca actatcatgg
    10861 cgtttttaat tttgccggaa agtatatctt ctctgcaaaa tccaaggtac ggaacacctg
    10921 aaggtatgtg accctgagta ggagcccagc ctgtcatacc atgtttagcc gtaaaattct
    10981 ccaattcctt tctgtctata tccccgcgtt ttactgccaa tgcacctatc attttataat
    11041 ttgccaaagg aacatctccc gctccggcag gttttgttac ttcaggattc tgcatttcta
    11101 cagaatattt gtctacatcc gttattttca ttcctgcttt atctaatgaa gatactaagg
    11161 atgttataac tgcctgagga gatgagcctg ttcctacagt gtgtcttccc acaaggtcaa
    11221 gattaatttc cggattcaca ccgtcatttt cgcctatcag cacagcaaac ccgccaatgg
    11281 tatcctctaa tatggggtat ccttttttaa tgtggtcctt accgttcatt cctaattttg
    11341 cagtgctacc ccctgcagat acaataactt ttttgaatac tcctgctttt accaatgctg
    11401 cagcttctac tatagcatga gtaggggctg cgcaaaaacc tcttgtatca caaccggtag
    11461 cattaacgag tcctgctact tcagcacatg actttgcaaa atttcctccg cctctttggt
    11521 tcatatcgcc gcatgcttct tcgctgcatt cgatgatgta ttcaatttct tctttgttta
    11581 tgttgttttt gcttataaga tgtaataaag caagaacatt tgaagctttt gatacaagat
    11641 tttcaaacat tacatgtccg cttaagttga tatcaatatc atgcgccctt ttaacgcatc
    11701 ctaccagcgc tccattatta tatataggct cggcatgctc tgcttccaca tattttttta
    11761 ttgtatcaaa ctcttctcct tctttgattc ttcctattaa actatcgtca ataagaggat
    11821 gattcataag ttttgcttta acagcggaaa cgaattgttt ttcaagttta actaaatcaa
    11881 atacatcaca gatttgcata agaactataa attcatcctg aggcataatt tcaccgaatt
    11941 taccttctct tgtcgctcct tccaaaggct tgtcaaacca aggcatttca tatttgctta
    12001 attcatcagg tgtaatattt cctatatatg tttgattagg caaataactt acgacttgtt
    12061 catacgttct cagatgattt ctaatttcct ttaaatactc ggaatttgga tttaccgttc
    12121 tttcggttgt ctgtgtagta ccgttattaa taaccatatc aggtgcaaaa accattatgt
    12181 aactgcttcc taaaattaca ctgttcataa aaacctcctt tatataaatt ttaatgatga
    12241 tccacatcat tccaaataat tgttcgtatt caaaatttgc ttcgcaggtt ctacgaagca
    12301 aattttttca tgttctattt cgtgttaaat aacgaggtca tacctcttcc ctcaacctta
    12361 taagtactta gaaaattctt ctcttattgc agtcatttca ctaattatgt catcaacatc
    12421 caataccatt tccatcatac ccacttgttc atcatatact gattcatcaa ccagctcttt
    12481 gatttccggt tcgcaaatat gaaatacgct gagtcccaac tgaactcctg tcaatggacc
    12541 tgcaaaagta gggtctccgt ttgttacggt ttctgcagca agacctgctg cttcgccttc
    12601 tgctgctcca agtataacca ctatattttc cgggccatac ttttcagcaa attctttaac
    12661 ccttttttga ttttccaaat ccattgcacc tgcgctagtt cagacaaagc attctgtgga
    12721 tgagaataca atttcgccgc cggcagtttt tacacattct tcaattgcct gaccgggtat
    12781 tccatctctg tcgcctatta tgataatttt cttgttctct aataagctca taatttcctc
    12841 ctatattatt ttgtatattt aaatgtattt ttgaatcatt gcttctatat tttcaggagt
    12901 tgcatcatct ttaaccaatt cttcaatttt ttctccatcc ttatatattg caattacagg
    12961 taaacctaat accttttgtc caatagctac ccttcttgct ttggtagtat tcaatttagt
    13021 aaattttaat ttatcaccat atttttccgc aagtttttca acatgaggca ttaaggcttc
    13081 acagggagca catccgtctc cgaaaaagtc aacaaatatg tatccttcgc cctgtaatac
    13141 ttctgtttcg aaatttgttt tatccaacac taacatagca tacctcctac agtgtttcaa
    13201 tatatttttc tgcctgaact gcagctattg ctccgtcagc tgccgcagta acaacctgcc
    13261 gtaagctttt aactctgaca tcgccggctg caaaaacacc ttcgatattt gttttcatat
    13321 tatcgtcggt tacaaggtat ccattttcga tatctaatat tccttcaaat attttagttt
    13381 ttggctcata accaatgaat acaaaaatac cgaatgtcat atcatcttca tcggcaacta
    13441 tttccgtcaa ttccttagtt ttaacatttt ctaaaactat tgaattgaca ataccatcac
    13501 cttttatttc ctttactacc gaatcccaga taaaatgtat tttgggattt gcttccgctt
    13561 tttctcgaat cgatttggca gctctaagct catttcttct gtgaataatt gatacttttc
    13621 ttgcaaactt agttaagtac aaagcttctt caacagcagt atcgccgccg cccactacaa
    13681 atacttctaa atcttcaaag aaagcagcat cgcaagttgc acagtatgat acccctttgc
    13741 ctgtgtatgt tttttctccg ggacatccga taggccttga tgtagcaccg cttgctatta
    13801 ttactgcttt gcctttgtat tctccctttt tgccgataag tattttttct ttgccttcaa
    13861 gcttcacttc cacaatttca tcatatactt tttctacgcc gaaatgctta gcctgttcag
    13921 ccatcctgtc aactaatgta ggtcctgatt ctccttctaa acacccggga taattttcaa
    13981 cttctgatgt gagaacaatt tgccctccat ctttttcttt ttcaataatt aatgtactaa
    14041 gccttgctct tcccgcataa ataccggctg ataatcctgc cggacctgct ccgataatca
    14101 atacatcata aactttactc atattccacc tcttttctgt ctaatattaa attggtaatt
    14161 atatacatac tgcttttatt cgcaaattta tttctaatgc cattgcaatg aataagcact
    14221 gtgattgtca tcctgagcat aagcgaagga tatcagtttt tataaagatg agattcttcg
    14281 agctaaagct ctcagaatga cacgtagctt attaatattt ttcttggtgt agtaaaaacg
    14341 tgataattat gagctaagtg aagaatctca cgcatattgc tgagatcctt cactttagat
    14401 caggataagc gactgactac aaatcaaaga tttgggtcat ctgtttactt ttcgaataca
    14461 gtttgctctg taacttcagt ttctaaagct tttaatgctc ttgtcagcaa ttttcttctt
    14521 atttcttttt cttcttctat agacaatgaa ggattcccca aggggtgagg tattgctatt
    14581 gtcggaacaa ttctgttagc tccaacagtt aatgaaatag gaactatagt acacatatgt
    14641 accacaggaa ttcccgctct ttcgatttct tttaccatcg ttgcaccgca acgtgtacag
    14701 gtacctcagg tagatgtgag tataactgc
//
LOCUS       DAOPUE010000041        14556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4229_length_14556_cov_8.982001, whole genome shotgun sequence.
ACCESSION   DAOPUE010000041 DAOPUE010000000
VERSION     DAOPUE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14556
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4229_length_14556_cov_8.982001"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PKH42_05245"
     CDS             <1..495
                     /locus_tag="PKH42_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOA19869.1"
                     /translation="EEVLEDNPAEEETEITENALIIKGHAEKELSLTLDELKAMDDII
                     FEADFYSLNNFGTTGYTHFKGVKLWDLLKNEALIKPEASKISIIAQDGYSMEFTIGQV
                     QMEYMDETNPENKYPMIIAWEENGEEYSLDEGNPYKLVVGQKEPGDINKPQWVSNIDV
                     ILIE"
     gene            504..2063
                     /locus_tag="PKH42_05250"
     CDS             504..2063
                     /locus_tag="PKH42_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA19870.1"
                     /translation="MKNRYILIILIILVIAGSLFYVLNDSSEEEEAVRLFYPDAKRVT
                     LIKGINDDLYSSLYFPAVKRAYEVDGEISAYVVSCVGYNGPVEVLAAIDDDKLIGIKI
                     LSHEESLDYAEHIEYDYFLDRFKNLPINKYLNLVVLDKENPEDIIQVTGATISSQAVV
                     NAVNAAIGSYMLWNYDIQMSKVPDVVPQEMWQKDINSFAINWEGGSIRIDTDEIKEYE
                     QLEMDVTLINTTGTETKMRVKGPTLHHVLEKEGIDLSEYAGIGVTGRDGYYTLIDKEK
                     LAVNDIILTWQVNRKNIKDEEKPIRISVPLELGPYWVKMVSNIDLYKEISPKDIDKVH
                     MFNSLTEDIEPYYYEYYGSKDKSIEVGKILRKFDVVDEKGFFTMAATDGLEKHETISL
                     VRQRYFLKVEGDNAPMNIAPNFKLGMNVKHMTHFSTTKDAVIFPEKMIEVVRTKSIEG
                     NDGMLLEDVLLTAGMRWNEGNKFTAVNKIEKIDLSLEEMLNCYLIYKEGQVSLYNDKE
                     IMTELSRIEKK"
     gene            2060..3217
                     /locus_tag="PKH42_05255"
     CDS             2060..3217
                     /locus_tag="PKH42_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA19871.1"
                     /translation="MKLSIRSIIQITATLIVIISCLIYGMYINEIIEFRILSIADLNP
                     YGGWSALKSLFTDLSYRWKGFSRSISLTLGIVAASFLMGRFFCGYICPIGAMQDFFKY
                     IGRKLRFKEVRFSNKPEILKYIFLIVIMVLSIVGMGNIVSPLSPWLAYLNIFAGLKLQ
                     TGAVILLFLIFVSLFGRRIFCRYFCPLGAFQSLIYAIGPLKIKKGSCNCSYCLKNCPV
                     SEEFRRTENEISPECINCLKCINSCIKNKEGFSITFMNKKINRLTYIVISIFIIAGSY
                     ILLPLTVSNSSVQAITSITGINDGTYEGFGFGFGGLINTEVTINNNKITDIRILNHSE
                     TAGYYEEVFRELAYEITETQNLNLDAISGATSTCRGFLNSLKDAVNKSLAD"
     gene            3226..3696
                     /locus_tag="PKH42_05260"
     CDS             3226..3696
                     /locus_tag="PKH42_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19872.1"
                     /translation="MQKKFIILIILIIVLSGCSKEKPSLNLNIDGKVESINTEIIYTH
                     KEALVFPAVVRSSGSKPVETEYKGIELIKLFDSLNVDITSYSKVTFNASDGYRIILSM
                     EEIKEPSNVYLTFERDGEILKSKKQGGNGPFQLVIRRDPFSQRWIKHVNEIILE"
     gene            3703..4254
                     /locus_tag="PKH42_05265"
     CDS             3703..4254
                     /locus_tag="PKH42_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOA19873.1"
                     /translation="MTNKFLEKFSVYNLIIIAVVSALGIATKPIVVPLVHIITGPLFI
                     PGGAIAGGFYMFWIVLGAGIVKKSGTGTLIGIVQGILVIATGTMGTHGIMSIVSYTLP
                     GIVTDIIFMFSINKKYNILHYVFGCLAANVTGTLISNILFFRLPFVTVILILSAAALS
                     GATGGLIAYAINRSLEKMKILED"
     gene            4257..5297
                     /locus_tag="PKH42_05270"
     CDS             4257..5297
                     /locus_tag="PKH42_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOA19874.1"
                     /translation="MKLKKILSAINYTEHSLGIKLFLLIFLVILVGCSADVNYKESTG
                     INIFNENKNFFVDFKSEKEISINEITNDKFSEAAVILNTGEIIQVPAEFYIKNNTVTA
                     DGETVENTVGIYLTDNYQSITNAYYDMKEYLDKGEKVMTVLLDGFSYNQFKRANEENT
                     LTFLSKYFQYPALSVYTPVTNAGFAAIITGQAPDVNGVHDRSNRRMKVDSIFKYALDN
                     NKSCILLEGDIKILNTEIEPELHTDINKNSETDDEIYESALISVNEDYDLIFIHFHGI
                     DDRGHSFGPYSREVMDYIKTIDKYIKDLSEEWEGAIILVPDHGMHETAEGGGHGICRQ
                     SDMVVPYFIKEP"
     gene            5294..5806
                     /locus_tag="PKH42_05275"
     CDS             5294..5806
                     /locus_tag="PKH42_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19875.1"
                     /translation="MKKRALIIVLLVIILTITLIISLNLNKASIEKQKQILSNAQIVL
                     KYNDKEKVLTKEEIILYGEDFEAVLDTSTTEPSTHIYRGVQLKDLLNNHNIDLQDKII
                     IIKAADGYSVAYSSEEVSIDKNVYIAFMEDGRYLGSRDSGGRGPYESIVVSDFFSNRR
                     CKWITEIEVK"
     gene            5803..6459
                     /locus_tag="PKH42_05280"
     CDS             5803..6459
                     /locus_tag="PKH42_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19876.1"
                     /translation="MMNAVEVKNLCFKYDNRKEYIFKDVNFNVRTGETIAITGRSGSG
                     KTTLCHCICGLVPRLYKGELTGEVLIFGENIKNLSIAEVVTKIGIIFQNPSTQLFSPT
                     IEDELAFGPENLCVEREKISSRIDEILRIIKMEKYRYDNPNNLSGGQQQLIAIASVLM
                     LKPSILICDEIMSWIDDEGRKIIKDLLLKLKKEGKTIILVDHDNDNLNIADRIIQIGE
                     "
     gene            6459..7112
                     /locus_tag="PKH42_05285"
     CDS             6459..7112
                     /locus_tag="PKH42_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19877.1"
                     /translation="MNNKIEINNISYEYPRGRKVFINFSLELNKGETTVITGKNGCGK
                     TTLAKLIMGILKPSFGEIKLFGQSTKDMSLGAIGEIIGYVFQSPERQLFGTTVLEELT
                     FPLLFKGYNREETMDRAEKMLEIFDLKKVRNSYPYFLSYGEKRCLAIASVLMVGPEYL
                     ILDEPTASLDKERIEALSQALENLKKRNIGMLIISHNKDFIERHKDRIINLEGGSYE"
     gene            7105..7839
                     /locus_tag="PKH42_05290"
     CDS             7105..7839
                     /locus_tag="PKH42_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOA19878.1"
                     /translation="MNNIDPRTKLIQVLILSTLALIYNEITLLSIILIAAIMIALINN
                     VNFISVIIRLNKLLKIIFLIALIQSLLTNMGNPIFKMGNFTLFTDYGILKSFEFILRM
                     GIIIVSAAIITTSSSREIIQGLIQWNCPYEIAFMVSVAVRFLPIFKEEMTDIITAIQL
                     RGIDLKKVKLSKKFQVYKYILTPITVNSIMKAKELAAAMEMRGFRAYSERTSYLVLKM
                     QKFDYILLGLSISAALLFIIITRGAL"
     gene            7836..8582
                     /locus_tag="PKH42_05295"
     CDS             7836..8582
                     /locus_tag="PKH42_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOA19879.1"
                     /translation="MKVFSVIGLTKSGKTTTIENIIKELRKRNYTVGSVKEIHFEKFK
                     MDVDGTNTHRHKMAGSQLVTARGAYETDVLFQRKLSMNEILCFYNHDFVILEGVRDTC
                     APKIVTAHDVEGIIERLDETTFAISGQISNSLTEYEGIPVINSLTEIEKLVDLIEKKV
                     FDRLPDMKDECCKKCGYTCTQLCSKILKCEAERRDCVLAENSVTLKINGQEIAMNPFV
                     QNILKNTVEAIAKELYGYTESGDIEVCIKR"
     gene            8567..9187
                     /locus_tag="PKH42_05300"
     CDS             8567..9187
                     /locus_tag="PKH42_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19880.1"
                     /translation="MHKEIKLRSKRNNVFRIVEDEGTYILKKFENHENYIREKEVLSI
                     LKKEGVNVPSIIKAEENYLYLEDLGEVNFLDWYEEQEKNNALDISMVYELCSWFKDFY
                     SAVFEFYKKQFILYDVNFKNFIICDNKVYGIDFEQVKPGHIEEDAGRLSAFALTYNPS
                     MTEWKMDFRNILINILSNELNIEKEKIISEENKELAAIKKRRGAFS"
     gene            9195..9827
                     /locus_tag="PKH42_05305"
     CDS             9195..9827
                     /locus_tag="PKH42_05305"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA19881.1"
                     /translation="MKSLLILIIILIISIVGCSSDKEIIEDINEEVLEISKDGISEDE
                     QTIEYSEEIHEENQQIPEEEEPLPELSAKEPLYDEESENDMTSAPADEPLAELEQTVP
                     TEEPLIEPEQTVPFEEAEEIISQPEEYIGEDRGIGGPIKVLVKMKDDKITDIEVVYHN
                     ETKGVGTNAIDTIISSIIENQTTDVDTVSGATVTSKALLNAVKNAIDKKE"
     gene            9889..10386
                     /locus_tag="PKH42_05310"
     CDS             9889..10386
                     /locus_tag="PKH42_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOA19882.1"
                     /translation="MYKEELAVISQAVLNEMEGYEFYKLAGEQAKTQGTKEAFMSLAN
                     DELKHAEYLQRLWKILSAGGEVKIEDILASGIEIPSPEIFRWDKVDKAYATLEMSIFR
                     IGMQMEKDSMDFYKEAKEKAKSESGKELFDLLIRWEKVHLDQFTNQYNVLKEEWWAEQ
                     RYFPF"
     gene            10530..12053
                     /locus_tag="PKH42_05315"
     CDS             10530..12053
                     /locus_tag="PKH42_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOA19883.1"
                     /translation="MLSKIKTCVLYGLEGYAVDVETDISGGLPNFNIVGLPDISIKES
                     KERVRSAIKNSRFDFPVSRITVNLAPANLKKEGSQIDLPIAVGILTASKIIKPVDEKI
                     CIIGELSLDGRITAIDGALPMVISMRNKSFNKAIVPEDNKEECGAIEGIEIIPVDSIT
                     QLVDYLNGNLSIAPYKSKYNFQDKPDYYNEDFSEIKGQPAMKRAVEIAAAGMHNILLL
                     GNPGSGKTMIARRIPTILPDLTFEESLEVTKIYSISGLLDNKGLITKRPFRSPHHTSS
                     RTSMAGGGPKPRPGEVSLSHYGVLFLDEIPEFPKSVIEVLRQPMEDGKISISRASGSL
                     TFPAKFMMVASMNPCPCGYLGDPTHECSCSQGQIDKYLGKISGPLLNRIDIQIEVLPV
                     KYDDLKDKRAEESSAVIKKRIVKARNIQRERYRNIGILTNSELSTKGISKFCKVNKES
                     EILLKNAFETLGLSVRAYNKILKVARTIADLENYENIETKHIAEAIRYRSLDRKYWR"
     gene            12055..13176
                     /gene="dprA"
                     /locus_tag="PKH42_05320"
     CDS             12055..13176
                     /gene="dprA"
                     /locus_tag="PKH42_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOA19884.1"
                     /translation="MDNLNINQKTLLWLSSGNVSNYNISKLLDYFGSVQNLWENFENE
                     QLNLNFLKVETVNKLSKIKIDFEERILEKLNSEKAQIVTIFDNDYPSKLEQIEGAPYL
                     LYYKGSLEDINNISIAVIGSRKATSYGKWAAEKLTKELSDIGVNIVSGLAVGIDTIAH
                     KTALKYNAKTYGIIGCGINIVYPKNNRELFKQIAENKGAVITEYTFDIQPVAFNFHDR
                     NRIISGLSEGVLVIEAQEKSGTLITAGHAANQGREIFAVPGNIDSLYSKGTNALIRDG
                     AKITVSIDDIAEEILELKERVSLKKTFIDLGTLTDDELKIIDCLKSGKNTIYVLEQET
                     KMETGELLGNLTFLEMKGAIKVSADKISLNLSFPSISNK"
     gene            complement(13165..14391)
                     /locus_tag="PKH42_05325"
     CDS             complement(13165..14391)
                     /locus_tag="PKH42_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOA19885.1"
                     /translation="MTQLFIVFIVFLMIPLLSRFKIQLSYILLIACCILALLSGMGAE
                     AALQSVKNTFTELSSVNTILSVMMVSVLGGLMKHYKILDKIVESIYKIIRNKKIILMV
                     LPALMGVLIVPGGALLSAPFIDEIGEEMKIPAPRRAAINLVFRHIAMFILPFSTGLLV
                     ISAALPEINISKLIFLNLFYVIPTTFVGYFLFIKDIKYENTQKVKLSGRNFLTLLLYT
                     SPIYICVIINSVTGLPFFVALIFSVLAVYLLCDKKQFFSILIKSVNYHTVLTVAAILI
                     MKELILNMEELIILFKSMFNAESSMLSILLVFLVSSVFFGIITGNQTATIAIIVPMIS
                     QLGISTDMIYIYTYFSFACSYAGYYFSPIHLCQAFTIQYMNVTTMELYKEYRLYVIVS
                     FIVPFVSVYLMKFLFV"
BASE COUNT         5385 a         2066 c         2847 g         4258 t
ORIGIN      
        1 gaagaagttt tagaagacaa cccggcagaa gaagagacgg aaattacaga gaatgcacta
       61 ataattaaag ggcatgcaga aaaggagctt tctcttacat tagatgaatt aaaggcaatg
      121 gatgatatta tttttgaagc tgatttttat tcattaaata atttcggtac aacgggttat
      181 acgcatttta aaggcgttaa gctgtgggac ctgttgaaaa atgaagcatt gataaaaccc
      241 gaagcttcta aaatatcaat tatagcccaa gatggataca gcatggaatt tactattggg
      301 caggtacaaa tggaatatat ggatgaaaca aatcccgaaa ataaatatcc aatgattatt
      361 gcatgggaag aaaacggaga agaatatagt cttgatgaag gtaatccgta taaattggtg
      421 gtaggacaaa aagagcccgg agatattaac aaacctcaat gggtttcaaa tatagatgta
      481 atccttattg aataggtgaa aaaatgaaaa acagatatat tctcattatt ttaattattt
      541 tggttattgc aggctcatta ttttatgtat taaatgattc ttccgaggaa gaagaagcag
      601 tgaggttatt ttatccggat gctaagagag tgacattaat taaaggtatt aatgatgatt
      661 tatacagctc cttatatttt cctgcagtta aaagagctta tgaagtagac ggtgaaattt
      721 ccgcatacgt tgtaagctgt gtcggatata acggacctgt tgaagtattg gctgcaatcg
      781 atgatgacaa attaatcgga ataaaaattt taagccacga ggaatcgctt gattatgcag
      841 agcatataga atatgattac tttttggaca ggtttaaaaa tctgcctatt aataaatact
      901 taaacttggt tgtgctggat aaggaaaatc ctgaagatat tattcaggtt acgggagcca
      961 ccataagctc gcaggctgtg gtgaatgccg ttaatgccgc aatcggctct tatatgcttt
     1021 ggaattacga tattcaaatg tcaaaagttc ccgatgtagt acctcaggaa atgtggcaga
     1081 aggatataaa cagctttgca attaattggg aaggcggttc tattcggata gatacagatg
     1141 aaatcaaaga atatgaacaa ttggaaatgg atgtaacctt aataaataca accggaacag
     1201 aaacaaaaat gcgcgtaaaa ggtcctactc ttcatcatgt gcttgaaaaa gaaggtattg
     1261 atttaagtga gtatgccgga ataggggtaa cgggaagaga cgggtactac actttaattg
     1321 acaaggaaaa actggcagta aacgatataa tacttacctg gcaagtaaac agaaagaata
     1381 tcaaagatga agaaaaaccg ataagaattt ccgttccttt agagcttggg ccttactggg
     1441 taaaaatggt ttcaaatata gatttataca aggaaatatc ccccaaggat attgataaag
     1501 tacatatgtt caattcattg actgaagata ttgagccata ttattatgaa tactacggct
     1561 ctaaggataa atccatagag gtcggaaaaa tattaagaaa atttgatgtg gttgatgaaa
     1621 agggcttttt taccatggct gccacggatg gtttggaaaa acatgaaaca atatcattgg
     1681 taaggcaaag gtattttcta aaggtagaag gtgataatgc tcctatgaat attgcaccga
     1741 atttcaagct tggaatgaat gtaaagcata tgactcactt ttctactaca aaggatgccg
     1801 taatttttcc cgaaaagatg attgaagttg taagaactaa atcaattgaa ggaaatgatg
     1861 gaatgctttt ggaagatgtg cttttgacag ccggaatgag atggaatgaa ggaaacaaat
     1921 tcactgccgt aaacaagatt gaaaaaattg atttatcatt ggaagaaatg ttgaactgtt
     1981 acttaatcta taaagaagga caagtaagct tatataatga taaagaaata atgactgaac
     2041 tttcaaggat cgaaaaaaaa tgaaacttag tataagaagc attatacaaa ttaccgcaac
     2101 tcttattgtg ataatatcat gtttgattta cggcatgtat ataaacgaaa taattgaatt
     2161 tagaattctc tccatagcag atttgaatcc atacggggga tggagtgctt tgaaatcttt
     2221 atttacggat ttatcttaca gatggaaggg gttttcaagg agtatttctt taactttagg
     2281 aatagttgcc gcttcatttt taatgggaag atttttttgc ggatatattt gtcctatcgg
     2341 agcaatgcag gattttttta aatatattgg acgcaaatta aggtttaagg aagtaagatt
     2401 ttctaataaa cctgaaattc ttaaatacat ttttttaatt gttataatgg ttttaagtat
     2461 agtcgggatg ggaaatatag tgtcccctct ttcaccctgg cttgcatact taaatatatt
     2521 tgccggatta aaacttcaaa caggagcggt aattttgctt tttcttatct ttgtatcatt
     2581 attcgggagg agaatattct gcaggtattt ttgtcctttg ggagcttttc aaagcctcat
     2641 atatgcaata ggaccgctga aaattaaaaa aggaagctgc aattgctcct attgcctgaa
     2701 aaattgtccg gtatcagaag agtttagaag aactgaaaat gaaatatctc ctgaatgcat
     2761 aaattgttta aaatgtatta attcatgcat taaaaacaaa gaaggctttt ctataacctt
     2821 tatgaataaa aaaataaaca gattaactta tatagtaata tctatcttta ttattgcagg
     2881 ttcatatatt ttacttcctt tgacggtatc aaattcttcg gtacaggcaa taacatcaat
     2941 taccggtata aatgacggta cctatgaagg atttggattt ggttttggcg gattaataaa
     3001 tacggaagta accattaaca ataataaaat aacagatata agaattctaa atcacagtga
     3061 gacagcaggt tattacgaag aagtattcag ggaattagcc tatgaaatca ccgaaactca
     3121 aaatttaaac cttgatgcca taagcggtgc tacatcaacc tgcaggggtt tcttaaactc
     3181 cttaaaggat gcagtaaaca aatccttagc tgattagagg ttattatgca aaagaagttt
     3241 attattttga ttatattaat tatagtttta tcgggctgct caaaagaaaa gccttcctta
     3301 aatttaaata ttgacggaaa ggttgaatca ataaataccg aaattattta tacccataag
     3361 gaagctcttg tatttcctgc agttgtaagg agcagcggtt caaaacctgt tgagacggaa
     3421 tataaaggaa ttgaacttat aaagcttttt gattctttaa acgtagatat aacaagttac
     3481 agcaaagtaa ccttcaatgc aagtgacggt tatagaataa ttcttagcat ggaagaaatt
     3541 aaagagcctt caaatgttta tttgactttt gagagggatg gagaaattct taagtcaaag
     3601 aagcaaggag gaaacggacc cttccagttg gttatcagaa gagacccctt cagtcaacgc
     3661 tggattaagc atgtaaacga aataatatta gagtaggttt ttatgacaaa taaatttttg
     3721 gaaaaatttt cagtttataa tttaattatt atagcagtgg tatcggcact cggaattgca
     3781 acaaaaccaa ttgtggtgcc cttggttcac attataacgg gacccttgtt tattccggga
     3841 ggagcaatag ccggcggttt ctatatgttt tggatagtct tgggagccgg aattgtcaaa
     3901 aaatcaggca cgggtacctt aataggcatt gtccaaggaa ttttggtaat tgcaacggga
     3961 actatgggaa ctcacggcat catgagcatt gtttcttaca ctctgcccgg tattgttaca
     4021 gatataatat ttatgttttc aataaataaa aaatacaata ttctccacta tgtgtttggc
     4081 tgtttggcgg caaatgttac gggtacgctt atttccaata tactattttt cagacttccc
     4141 tttgtaacgg ttattttaat cttgtctgca gcagctttgt ccggagcaac aggcgggctt
     4201 atagcttacg ccattaacag aagtttggaa aagatgaaaa tattagagga ttagaaatga
     4261 agctgaaaaa aatattgtcg gctattaatt atacagaaca tagtcttggg attaaactat
     4321 ttttattaat atttttggta atcttggttg gatgcagtgc tgatgtaaac tataaagaat
     4381 caacaggcat aaatatattc aacgaaaata aaaatttttt cgttgatttt aagtctgaaa
     4441 aagagatttc aataaacgaa ataacaaacg ataaattttc agaagcagcg gtaattttaa
     4501 atacaggaga aattattcaa gtgcctgccg aattctacat aaaaaataat acagttacag
     4561 ctgatgggga aacagttgaa aatacagtag gaatttattt aaccgataat tatcaaagca
     4621 taacaaatgc atattatgac atgaaagaat atttggataa aggcgaaaag gttatgacgg
     4681 ttcttttaga cggcttttcc tacaatcagt ttaaaagggc taatgaagag aatactctta
     4741 cttttttgag caaatatttc caatatcctg ccttaagtgt atacactcct gttacaaatg
     4801 caggatttgc agcaatcatt acgggacaag cccctgatgt aaacggagtt catgacagaa
     4861 gtaatcgccg gatgaaagta gacagcatat tcaaatatgc tcttgataac aataaaagct
     4921 gtattttgct tgaaggagat ataaaaatat taaataccga aattgagcct gaacttcata
     4981 cagatattaa taaaaacagc gagaccgatg atgaaatata cgaaagtgca ttaatttccg
     5041 ttaacgaaga ctatgatttg atttttattc attttcacgg cattgatgac agaggccaca
     5101 gttttggacc atattcaaga gaagtgatgg attatataaa aactatcgat aaatatataa
     5161 aagacttaag tgaagagtgg gagggagcaa taatacttgt gcccgaccac ggtatgcatg
     5221 aaactgccga aggaggagga catggaatat gccgtcaatc agatatggtt gtgccttatt
     5281 tcattaaaga gccatgaaaa aaagagcttt aataattgta ttattagtaa tcatattgac
     5341 aattacatta ataatatctt taaacttaaa caaagcctcc atcgaaaaac aaaaacaaat
     5401 attatctaat gcgcagattg tattgaaata taacgacaaa gagaaagtat taacgaagga
     5461 agaaataatt ctttacggtg aggactttga agctgttctt gacacatcga ctactgaacc
     5521 gtcaacgcat atatataggg gtgttcagct gaaagacttg cttaataacc acaatattga
     5581 tttacaggac aaaattatta taatcaaggc ggccgacggc tattctgttg cttattcatc
     5641 ggaagaagtt tctattgata aaaatgtata tattgctttt atggaagacg ggagatatct
     5701 tggcagcagg gactcgggag gaagaggtcc ttatgaatcc atagtagttt cggatttttt
     5761 cagcaacaga agatgcaagt ggattactga aatcgaggtt aaatgatgaa tgcagttgaa
     5821 gttaaaaatt tatgctttaa atatgacaac cggaaagaat atatttttaa ggatgtaaat
     5881 ttcaatgtaa gaacaggtga aacaattgca ataacaggac gaagcggaag tggcaaaacc
     5941 accttgtgcc attgtatctg cgggcttgtg ccgagattat acaaggggga attaaccgga
     6001 gaggtcttga ttttcggtga aaatataaaa aatttaagta tcgcggaagt agtgacaaag
     6061 ataggaataa tatttcaaaa tccttcaaca cagctatttt ctcctacgat tgaagatgaa
     6121 cttgcatttg gtcccgaaaa tctatgtgtt gaaagagaaa aaataagctc taggattgat
     6181 gaaatattaa gaattataaa aatggaaaaa tacagatatg acaatccgaa caatttatcg
     6241 ggaggtcagc agcagctaat tgcaattgca tctgtactaa tgcttaaacc ttcaattctc
     6301 atatgcgatg aaattatgtc atggattgac gatgagggaa gaaaaataat caaagatctt
     6361 ttactgaagt taaaaaaaga aggaaaaaca ataattctgg ttgaccatga caatgataat
     6421 ctaaatatag ccgataggat tatacaaata ggagaataat gaacaataaa attgaaatca
     6481 acaatatttc atatgaatat ccgagaggaa gaaaggtatt tataaatttc tctctcgaat
     6541 taaataaagg ggaaacaact gttataacgg ggaaaaacgg ttgtggaaaa acaaccttgg
     6601 ctaagttaat tatgggaata ttaaaacctt cattcgggga aataaaacta ttcggtcaaa
     6661 gcacaaaaga tatgtccctt ggagccatag gtgaaattat aggctatgtt ttccagagtc
     6721 ccgaaaggca gctttttggc actaccgtat tggaagaatt gacatttcct ttgttgttta
     6781 aaggatacaa cagggaagaa actatggata gggctgaaaa aatgctggaa atttttgatt
     6841 tgaaaaaagt tagaaattca tacccctatt ttttaagtta cggagaaaaa agatgtttag
     6901 caattgcttc cgtattgatg gttggtcctg aatatcttat tttggatgag cctacggctt
     6961 ctcttgataa agaaagaata gaggcattat cgcaagctct tgaaaatcta aaaaaaagga
     7021 atatcgggat gcttataata agccataaca aggattttat cgaaaggcat aaagacagaa
     7081 ttattaatct tgaaggggga agttatgaat aatatagacc cccggacaaa attaatacaa
     7141 gttttaattc tatcaaccct tgcattaatt tataatgaaa taacactttt atcaatcata
     7201 cttattgctg caattatgat tgcacttatt aacaatgtaa attttatatc agtaattata
     7261 agacttaata agttattaaa aataatattc ttgattgcgt taattcagag tttattgaca
     7321 aatatgggaa atcctatttt taaaatgggt aattttacgc tttttaccga ttacggaatt
     7381 ttaaaatcat ttgaatttat tttgcgcatg ggaataatca tagtttctgc tgcaataata
     7441 acaacatcat caagcaggga aataatacag ggacttattc agtggaattg tccttatgaa
     7501 atagcattca tggtttctgt cgcagtaagg tttttaccta ttttcaaaga agaaatgaca
     7561 gatataataa cggcaataca actaagagga attgatttga agaaggttaa acttagtaag
     7621 aaatttcaag tttacaaata tatacttact ccaataaccg ttaattcgat tatgaaggca
     7681 aaagaattgg ctgcagcaat ggagatgaga ggttttagag cttactccga aagaacaagc
     7741 tatttggttt taaaaatgca gaaatttgat tatattctat tagggctgag catttcagca
     7801 gcattattgt ttattattat aacgagaggt gcattatgaa agttttttca gtaatcggac
     7861 tgacaaaatc cggcaagact actaccatag aaaatataat aaaggaactt aggaaacgta
     7921 attacactgt cgggagtgta aaagaaatac attttgagaa atttaaaatg gatgttgacg
     7981 ggactaacac tcacaggcat aaaatggcag gctctcaatt ggttacggca agaggcgcct
     8041 atgaaacaga cgttttgttt caaagaaaac taagtatgaa tgaaattctt tgtttttaca
     8101 atcatgattt tgttatcttg gaaggagtaa gggatacatg tgctcccaaa atagtaacag
     8161 cccatgatgt ggaaggaata atagaaaggc ttgatgaaac aacatttgca atatcaggac
     8221 aaatatccaa cagtttaacg gaatatgaag gtatacccgt aataaattcg ttaacggaaa
     8281 ttgaaaagct ggtagattta attgaaaaaa aagtatttga cagacttccg gacatgaagg
     8341 atgaatgctg taaaaaatgc ggatatacat gcactcaatt atgctcaaag attttaaaat
     8401 gcgaggcgga aaggagggat tgcgttttgg ctgaaaacag tgtaaccctt aaaattaacg
     8461 gacaggaaat tgctatgaat ccgtttgttc agaatatttt gaaaaataca gtggaagcaa
     8521 ttgcaaagga gctatacggt tatacggaaa gcggagatat cgaagtatgc ataaagagat
     8581 aaaactaaga agcaaaagaa ataatgtatt tcgaatagtg gaagatgaag gaacatatat
     8641 tctgaaaaaa tttgagaacc acgaaaatta cataagggaa aaagaagtgc taagtatttt
     8701 aaaaaaagaa ggagtcaatg ttccttcaat aattaaagca gaagaaaact atctatacct
     8761 ggaagatttg ggagaagtta attttcttga ttggtatgag gaacaagaaa aaaataatgc
     8821 tttggacata agcatggttt acgagctttg cagttggttt aaagattttt attctgcagt
     8881 ttttgagttc tacaaaaaac aatttatatt atatgatgta aattttaaaa attttattat
     8941 ttgcgataat aaagtttatg gaattgattt tgaacaggta aagccgggtc atatagaaga
     9001 agatgcaggc agattatcag cttttgcatt gacttacaac ccttctatga cagaatggaa
     9061 aatggatttt aggaatattt taattaacat tttatctaat gaattaaata ttgaaaaaga
     9121 aaaaattatt tcggaagaaa acaaggaatt ggcagccatt aaaaaaagaa gaggtgcttt
     9181 ttcttgaggt gactatgaaa agcctattaa ttttaataat aattttaata atttctattg
     9241 tcggatgctc atcggataaa gaaataattg aagatataaa tgaagaagta ttagaaatca
     9301 gcaaagatgg tatatcggaa gatgaacaaa caattgaata ctctgaagaa attcatgaag
     9361 aaaatcaaca aatacctgaa gaagaagagc ctttgcctga attatctgca aaagagccct
     9421 tatacgatga agaatccgaa aatgatatga cttctgcacc tgccgatgag ccattagcgg
     9481 agttggaaca gacagtgcct accgaggaac cgttaataga gccggaacag acagtgcctt
     9541 tcgaggaggc cgaagaaata atttcacagc cggaagaata cataggcgag gatagaggta
     9601 tcggcggacc cattaaagtc ttagtcaaaa tgaaagatga taaaataacc gatattgaag
     9661 ttgtttatca taatgaaact aaaggtgttg gtacaaatgc tatagacaca ataatttctt
     9721 ccataataga aaatcaaaca accgatgtcg atactgtttc gggagctacg gttacgagca
     9781 aggcactttt gaatgcagta aaaaatgcta tcgataagaa agaataaaga ataatgttta
     9841 agaaattcct ccaatggtat aatagtaata ataaaaagga ggaattttat gtataaagaa
     9901 gaattggccg ttatttcaca ggctgtttta aatgaaatgg aaggttacga attttataag
     9961 ctggcaggag agcaagctaa aacgcagggt acaaaagaag catttatgtc tcttgcaaac
    10021 gatgaattga agcatgcaga atatttacaa agattatgga agatactcag tgcaggcgga
    10081 gaagtaaaaa ttgaagatat attggcttcc ggaattgaaa taccttctcc ggaaattttt
    10141 cgctgggata aggtcgataa agcatatgca acattagaaa tgtctatatt taggatagga
    10201 atgcaaatgg agaaggactc catggatttt tacaaggaag ccaaggaaaa agcaaaatcg
    10261 gaatcaggca aggagctgtt cgatttgctg attagatggg aaaaagtcca tttagaccaa
    10321 ttcaccaatc aatacaacgt attgaaggaa gaatggtggg cggaacaacg ttatttcccg
    10381 ttttaaccta agtttatgta ttctcctctt taatgcaata ggtgttaaag gggagatttt
    10441 tattgcaaaa ctataattaa agatttatat taattgaaat aattggaaaa ttatagtata
    10501 attttataac ttcaattatc ggaggtatta tgctttcaaa aattaaaaca tgtgttcttt
    10561 acggacttga aggttatgca gtcgatgtgg aaactgacat atcgggaggt cttcctaact
    10621 ttaatatagt tggcttgcct gatatttcaa ttaaagagtc aaaagaaaga gtacgctctg
    10681 caattaaaaa cagcagattc gattttcccg taagccgtat tacggtaaat cttgcacctg
    10741 ccaacttaaa aaaagaaggc agtcaaatag accttcctat tgcagtgggc atacttactg
    10801 cgtccaaaat tataaaaccc gttgatgaaa aaatatgcat tataggagaa ttatccttgg
    10861 acggtaggat aactgccatt gacggtgctt taccaatggt tatttccatg cggaacaaga
    10921 gctttaataa agcaatagtg cctgaagaca ataaggaaga atgcggagca atagaaggta
    10981 tagaaataat tcccgtagat agtataactc aattggtaga ttatttaaac ggtaatttat
    11041 caattgctcc atataaatca aaatacaatt ttcaagataa accggattat tacaatgaag
    11101 atttttctga aataaaagga cagcctgcca tgaaaagggc agttgaaata gctgcagccg
    11161 ggatgcacaa tatactcctt ttaggcaacc ccggctccgg caaaacaatg attgccagaa
    11221 ggattcctac cattttaccg gatctgacat ttgaagaatc tttggaagta accaaaatct
    11281 acagcatatc aggattgttg gacaataaag gtttaataac aaaaagaccc tttcgttcac
    11341 ctcatcatac ttcttcacgt acatccatgg caggaggcgg cccaaagccg aggccgggag
    11401 aagtatccct gtctcactat ggtgttttat ttttggatga aattccggag tttccaaaaa
    11461 gtgttattga agttctgcgt cagcctatgg aagacggaaa gatttcaatt tcaagagcaa
    11521 gcggttccct tacatttcct gcaaagttca tgatggttgc ttccatgaac ccatgtccgt
    11581 gcgggtattt aggcgaccct acacatgagt gctcctgcag ccaaggacaa atcgataaat
    11641 atttaggaaa aatttcaggt cccttgttga accgaattga tattcaaatt gaagtgctgc
    11701 ccgttaaata tgatgattta aaagataaga gggctgaaga aagttcggcg gtaattaaaa
    11761 agcgaatagt aaaggcgaga aatattcaga gagaaagata cagaaatatt gggattttga
    11821 ctaattcgga gctttcaacc aaaggcatat ccaagttttg caaagttaat aaggaatctg
    11881 aaattttgct taagaatgca ttcgaaacat taggcttaag tgtaagagca tacaataaaa
    11941 ttctaaaggt tgcaaggaca attgctgact tggaaaacta tgaaaatatt gaaacaaagc
    12001 atatagcgga agcaattcgg tacaggagtt tagaccgcaa atactggagg taaaatggat
    12061 aatttaaata ttaatcaaaa aacattatta tggctttcat ccggcaacgt gtcaaattac
    12121 aatatttcaa agctcctcga ttatttcgga tcggtacaaa atttatggga aaactttgaa
    12181 aacgagcaat taaatttgaa ttttcttaaa gttgaaacag taaataagct ttccaaaatt
    12241 aaaattgatt ttgaagaaag aatattggag aagttaaata gtgaaaaggc tcaaatagta
    12301 actatttttg ataatgatta tccttctaaa ctagaacaaa ttgaaggtgc tccttatttg
    12361 ttatactaca aaggcagttt agaagatatt aacaatatat caattgcagt cataggctcc
    12421 agaaaagcta caagctatgg aaaatgggct gcagagaaac tcacaaagga attgtcggat
    12481 attggtgtaa acatagtaag cggtctggca gtcggaattg atacaattgc tcataaaaca
    12541 gctttgaaat acaatgccaa aacatatggt ataatcggtt gcggcataaa tattgtatac
    12601 ccgaagaata acagggagct ttttaaacaa atagccgaaa ataagggagc tgtaattacc
    12661 gaatatactt ttgatataca accggttgct tttaattttc atgacaggaa tagaattatc
    12721 agcggacttt ccgagggagt attggtcatt gaagctcagg aaaaaagcgg tacattgatt
    12781 acagcgggtc atgcagctaa tcagggaaga gaaatatttg cggttcccgg aaatatagac
    12841 agcctgtaca gcaaaggcac caatgctctt atcagagatg gagctaaaat aacagtatca
    12901 atcgatgata ttgcggaaga aattttagaa ttgaaggaaa gagtaagtct taaaaaaaca
    12961 tttattgatt taggcacatt aaccgatgac gaactaaaaa ttatagactg tcttaagtca
    13021 ggtaaaaata caatatatgt attagagcag gaaacaaaaa tggaaaccgg ggaattgctt
    13081 ggcaatttaa cattcttgga aatgaaaggt gcaataaagg tgagtgccga taaaatttct
    13141 ctgaatttaa gtttcccttc gatatcaaac aaataaaaat ttcatcaaat atactgaaac
    13201 gaagggcact atgaaagaaa caattacata cagcctgtat tccttataga gctccattgt
    13261 tgtaacattc atatattgaa ttgtgaaggc ctgacacaag tgaatcggtg agaaataata
    13321 acctgcatat gagcatgcaa aagaaaaata tgtatagatg taaatcatat cagtagatat
    13381 accaagctga gaaatcatcg ggacaataat tgcaatggta gctgtttgat tacccgttat
    13441 aattccaaaa aatacggacg atactaagaa taccaataaa atcgacaaca tgctgctttc
    13501 tgcattaaac atacttttga ataatattat aagttcttcc atatttaaaa taagttcttt
    13561 catgattaga atcgctgcaa ccgttaatac agtatggtaa tttacagatt taatcaatat
    13621 agagaagaat tgttttttat cacataataa atagacagct aatacactga aaatcagagc
    13681 aacaaaaaag ggaagacctg ttacggaatt aataattaca caaatatata ttggagatgt
    13741 ataaagtaaa agagtcaaaa aatttcttcc tgataatttt actttttgtg tattttcgta
    13801 tttaatatct ttaataaaca gaaaataacc aacaaatgtt gttggtataa cgtaaaacag
    13861 gtttaaaaaa ataagttttg atatatttat ttccggaaga gcagcggaaa taaccaatag
    13921 tccggttgag aagggcagta taaacattgc aatatgtctg aataccaaat tgatagctgc
    13981 tcgtctggga gcaggaattt tcatttcttc gccgatttca tctatgaaag gtgcggataa
    14041 aagggcacca ccgggaacta ttaatacacc cattaaagca ggaagaacca tcaatataat
    14101 ttttttgttt cgtattatct tataaataga ttccactatt ttatcaagaa ttttataatg
    14161 cttcattaag ccgcctaaaa cgcttaccat cataactgat agtattgtgt ttactgatga
    14221 aagttccgta aaagtatttt ttaccgactg caatgcagct tctgctccca ttccggacag
    14281 caaagctaaa atacagcatg ctatcaaaag aatataactt aactgaattt taaatctact
    14341 taataaaggt atcattaaga aaacaatgaa tacgataaat aattgagtca tacgtccctc
    14401 cgtgcaaatt aacataatta tataaaacta ttgagaataa atcaatatat tattagacat
    14461 gagcattcct aaccgcatga taataaatct taacaatcaa aaaggtgaat tgacaaagta
    14521 agtgcaactt ttttagtaaa aaagatggtg gatagc
//
LOCUS       DAOPUE010000042        14510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4249_length_14510_cov_9.408094, whole genome shotgun sequence.
ACCESSION   DAOPUE010000042 DAOPUE010000000
VERSION     DAOPUE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_14510_cov_9.408094"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKH42_05330"
     CDS             complement(<1..240)
                     /locus_tag="PKH42_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOA19886.1"
                     /translation="MNKRKIKIIKNGPYKVTGNVPMREKIIVPKGNQYEYRDGKEFPD
                     DDSYLLCRCGHSKKHPFCDGTHSKINFDGTETASKK"
     gene            435..1349
                     /locus_tag="PKH42_05335"
     CDS             435..1349
                     /locus_tag="PKH42_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOA19887.1"
                     /translation="MKINKIALIGMGAVGTVYGSLLYNKYGSNFTVISDESRKDKLKK
                     NGFTLNGNTFYPNVSSNSDNTIKYDLIIFAVKNYQLAGALEDVRSFVGENTILITFLN
                     GVTSRDIIQGAYPNNKVLYGLAMRIDAVRNEAGVVNTEDGEIHFGEADNRVISEEVQA
                     VKDCFDAAGIRNIVFPDMIRMYWKKFMLNVGFNQVTAVTRAPYGKVTNIETSLILFKE
                     AMNEVLTIAKALNIDLREEDIEEFVALMDHFSPFGKTSMLQDVEAKRKTEVDYFGGTV
                     VELGKKLNIPTPVNHVLYCIIKSLEELY"
     gene            complement(1441..2553)
                     /locus_tag="PKH42_05340"
     CDS             complement(1441..2553)
                     /locus_tag="PKH42_05340"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOA19888.1"
                     /translation="MLYITKNHLKPGMVLAKDIYLYNRRNFNTFLLAKGQVLSSTYIN
                     RINFHNIDGVYIENDAFSDINVEPYIADDLKAESLQRISDTYYEFQVSSGKINDYMIN
                     RLSKTVKNIIIELLNKDDLSYNIMDFKNYDSYTFQHCLNVAMLSISTGISLKLSEQDL
                     LDLGLAGMLHDIGKMLIPIEILNKPSTLTKEEFEIIKTHPVNASKQLRNMVPHEVLRA
                     VEDHHERLDGTGYPHEKRDNNISLYARILSISDVYDSLISDRPYRKSIFPNEVVEYLM
                     GCADKQFDYNILKHFLKIIFAYPVGTFVKLSNEELAVVIKNHSENILRPIVRIIKNDG
                     TVGEDVDLLYDKDYMNITIIDMGYDLLSMYPLADSY"
     gene            complement(2588..3634)
                     /locus_tag="PKH42_05345"
     CDS             complement(2588..3634)
                     /locus_tag="PKH42_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOA19889.1"
                     /translation="MNENQNNIQYYDDEIDLRELIMALWKRKKMIIAFTLAVAILAAS
                     FSMFILSPVYKTSLDIIISMPEIYNTRYGEYKLPITTSEQYINLISSDDVLINTIKDM
                     NYDTEEISVENLNKRIAVIASNTKANTVQNSFTVTVSADNPEESHKLAKSLFENYIEF
                     LDVMVKERAINHYLNLFSVELMALQNTLDVEKETLAKNEELLSQTPKVINTKSNVEIQ
                     SPLNDESNYIIPIETANPNYIKIENDIIQNKQNINSIESSINKTMEYIEELNVEKQAV
                     SKYYKNGRTEKLETSVIGIVENNVYLASQPAIPTQKSSPNNSLNTIIGAVLGGMVGVM
                     AALFMEYWFKKENN"
     gene            complement(3720..5000)
                     /locus_tag="PKH42_05350"
     CDS             complement(3720..5000)
                     /locus_tag="PKH42_05350"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOA19890.1"
                     /translation="MESLKHVKLGILGMGTVASGLINIMEFNKDKVHGNINGELMVNK
                     VLVNNPNKIRNVNLPKEVYTTDAYEVINSDADIIVELIGGLSPAYEYIKAALNNKKHV
                     VTANKALIATYGEELHILAEKNNVKLMYEASVGGGIPILNTIRDNLCANEFEYIFGII
                     NGTTNYILTQMTENGLDYRQALKEAQALGFAEADPSSDVEGDDAAYKLSILSTLAFGQ
                     RIHLKDIPKEGITKISKEDIEYAKELGYNIKLLAAGIKKGNEIELNVQPAFIPAAHPL
                     STVKNEFNAVFLKGNAVGELMLYGKGAGSLPTGSAVLGDIIHIVKNAGSSYAKEGEKK
                     YVISNKGFKQYYINMKVIDKPGVLGSIASIFGKNKVSLASVVQRQKNGNDTVPLIFIT
                     HETERKNIDAALEETKKFDFVTELDSIIIVENFK"
     gene            5621..5983
                     /locus_tag="PKH42_05355"
     CDS             5621..5983
                     /locus_tag="PKH42_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008800843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOA19891.1"
                     /translation="MGKFVVKEVKTGFKFDLKATNGQVIASSEIYKTKASCLKGIDSV
                     INNAPIANLEDQTVENFKVEKNPKFEIFTDKAGEYRFRLKAKNGEIIATSEGYKAMDS
                     CKNGVESVRKNAANSTIE"
     gene            6124..6780
                     /locus_tag="PKH42_05360"
     CDS             6124..6780
                     /locus_tag="PKH42_05360"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HOA19892.1"
                     /translation="MKKSVKKIFLYVSGLLILAMGVNVSKAAQLGISPVSAIPYALEL
                     IWNIELGKAVFIFNIFLIALQIALLRKNFKPIQLLQIVCTFLFGLFVTYTGKEFLLFW
                     LPIPSLYIIKLLYLFVSIVIIGIGVSFYLIPDYIPLPAEGTMNSIVELSKGKLKFSNV
                     KVSVDSSMVAVSALLSIIFLGQLKSVREGTIIAALLIGKVVGVVFKRYKPQIVEWIEK
                     "
     gene            6948..8039
                     /locus_tag="PKH42_05365"
     CDS             6948..8039
                     /locus_tag="PKH42_05365"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOA19893.1"
                     /translation="MKYICSKCNTRMDVATKKSKCDCGGLWKLDYKPPKFDLSLVDKN
                     NWSIFRYRAFMPLLGNEWEKISLGEGLTPVIQFNEDVLLKMDYFMPTLSFKDRGAAVL
                     IAHCKAIGVDSVVQDSSGNAGNSVAAYCAKAGIDCEIFVPEGTSPKKIDMIKSHNAKV
                     NVVPGSRDNCANVCRAKSDKEGKYYANHVYNPFFYEGTKTYIYEVYEQLNRIPENIFI
                     PLGNGTLFIGIIKALEEFMESNIIDKMPNIIAVQSEYCDPFTKAFKIKASKPVKVISK
                     PTMAEGIAIGEPMRGEEILKYIYKYNIEVINSPETGILEASALLASKGIYVEHTTAAT
                     YAAYMKYCELKGRTPALIPMCGAGLKSDH"
     gene            8171..8758
                     /locus_tag="PKH42_05370"
     CDS             8171..8758
                     /locus_tag="PKH42_05370"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOA19894.1"
                     /translation="MAIKIFIDQGHNPPGFHNAGAEGNGLNEGEINFRAGIYLGNLLA
                     NDYRFEYMLSRPTPTTVLGTNTSTSLAERVRMANQWPANYFISLHCNANVNPSINGSE
                     MYIYQFNTQANWLAQHIMNGIVRNTRLRDNGIRENKSLYVLRNTNMPAVLVEMGYLTN
                     FNDAMILRDNLWNVAYGIYLGILNYFGFLPLNNIG"
     gene            complement(8806..10197)
                     /locus_tag="PKH42_05375"
     CDS             complement(8806..10197)
                     /locus_tag="PKH42_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOA19895.1"
                     /translation="MSIEIPGLTQVVIPAAITVHLGAPDEPGENVTVPFLDYIKNVAS
                     SELYPTWPEAALRANIYAITSTALNRIFTEWYRAQGYNFDITNDTRFDQAFVHNRGIF
                     DTVSVLADELFDSYIIRRGQIQPLYAQFCDGRVSWCPGLLQWGTVELAEQGYTPYEML
                     QYYYGEDIDIKTDTPIAEAFETYPGISLQLGDNNPYVLLMQIRLNTIATNYPAIQRIP
                     NPTGTFDEATQEAVRQFQDIFNFPVTGIIDKSTWYGIRRIYAAVTKLAELTSRGIMLS
                     EIPEFTPTPGPEEVIPRVQIVQYFLNVLSAYYNSIPSVDISGVLNTQTRTALMEFQKT
                     FNLPITGLVDEQTWNTMFNAVSGILETLPPSAIALPALLWPGITYRVGSEGPGVYLLQ
                     QYLSYIASVLEGITPTDPDGIFGPQTELAVRTFQEFFGIDVTGVVDRYTWDRIVLIYR
                     NLRFGDTSRVQGS"
     gene            complement(10208..10675)
                     /locus_tag="PKH42_05380"
     CDS             complement(10208..10675)
                     /locus_tag="PKH42_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOA19896.1"
                     /translation="MKYNPFDGRIYKAQVPPGSIPFEQEVGYLSVSVFTASGALPVEG
                     ALVTVYIRDESGAEHEIVHHITDANGRIPLITLPVMYDRLDPFESGRYYFTTYNLRVQ
                     AINYYTVNVIDLRIFPNTTTRFQINMIPVAIGPVEGPDTTIVIPPSPIDISND"
     gene            10864..12066
                     /locus_tag="PKH42_05385"
     CDS             10864..12066
                     /locus_tag="PKH42_05385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA19897.1"
                     /translation="MLARAFKLIKNLFSTSKTDDCKKEFAIDFCRINLAKLIISAKTL
                     IALGIIQLIVYCIVSKGNLFTKPQIYFGAMNFILLFAMTIFLSVIVKLEKDAAKNVVS
                     IHNAQIFFINFILLWSGGISLLHQLTHGQTIVYIAAVIITAGALYIEPSVSFLIYFTS
                     HLIFSVLLPNFQKSPDLLFGIYLNTTSLVIMSFAISYTRYKRQEQDFKNRKIMQENSD
                     ELKRINKELEEANKKLKIISQYDGLTGIFNRHTFDTKIREEWNRCKRHSMPLSLIMAD
                     IDHFKSFNDKFGHQAGDNCLKLVAEILSECAKRSSDVVARYGGEEFVILLPQTERERA
                     YDLAEKMRKRVEELNITISLGVNTTIPSDESSVREFIKNTDKALYIAKNKRNSTEVYL
                     PIDTFGFS"
     gene            complement(12037..12990)
                     /locus_tag="PKH42_05390"
     CDS             complement(12037..12990)
                     /locus_tag="PKH42_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOA19898.1"
                     /translation="MTKVKKAVYYICDNHDWGYVSYLVWDILKEEGYLNNKVDFTFDG
                     REVYKYIDNKNNEFYFVPTDIALCRDYPRYLPDMNRYFSDFDMSGMVTWHEGTNALDK
                     VITVHSIGDVNSGCYGPANPLYMHNLMWAMEKLRKEIELDFSVVTEATHWSGVYEGQG
                     DPELLLKYPVPIVDIEIGSTPESWVNMDACRVLALSLLHIFDKDIDDIKLHNLLCVGG
                     VHFDPNFAEAVHTCWNENEFFGVSHIIANQWLVSGEYEGENGIEKASECVDAIIGGID
                     AIVFHDKLKGCYKDVVRALGEKYKVPILKHQALRKPESINW"
     assembly_gap    13089..13188
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13189..13620
                     /locus_tag="PKH42_05395"
     CDS             <13189..13620
                     /locus_tag="PKH42_05395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA19899.1"
                     /translation="ARKFMKASGNPNQWKDSYPERTLIESDIENNISYVCVDEGRIVG
                     TFVYFKGIEPDYAEIHDGQWLNEEPYGVVHRIASATGKRGVATYCLNWSFEQCRNLKI
                     DTHHDNIPMHNLLTKNGFNRCGIIYLENGDERIAYQKADLA"
     regulatory      13705..13809
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13924..>14510
                     /locus_tag="PKH42_05400"
     CDS             13924..>14510
                     /locus_tag="PKH42_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOA19900.1"
                     /translation="MKNNFKLETALVQSLEEFQEGEARVYPLVQSTTYNYKDPDYVAA
                     LFDLTESGHMYSRISNPTVEAFENKMAHLEGGVGAVAVSSGMAATTMAITNICNSGDH
                     VIALSTIYGGTFTLLSSSLKKFGIETTFFGPDSTEEEILSLVQDNTKLIFGETIGNPG
                     MNVLDFKTVSNAAQKAGLPFIVDNTIATPYLCRPI"
BASE COUNT         4630 a         2499 c         2597 g         4684 t
ORIGIN      
        1 tttttttgaa gcggtttcag ttccgtcaaa gtttatttta ctgtgggttc catcgcaaaa
       61 agggtgtttt ttggagtgtc cgcatctgca aagcaaatag ctgtcatcat cagggaattc
      121 ttttccatcc ctgtattcat attgatttcc ttttggaaca attatttttt ctctcatagg
      181 cacatttcct gtaaccttat agggaccgtt ttttataatt tttatttttc tcttgttcat
      241 ggcagcatac ccttcgtttc cgttagaata tcggtgccag gcaccaaaaa tccaaagacc
      301 aaagtctttg cttcattatt tattataaca tacatttcaa aaatatagta aaataaattt
      361 attcattgca tttaaaggat aactttatta taatcaataa ctatgaaatt aaataaatat
      421 tataggtgat gaacatgaaa ataaacaaaa ttgcattaat aggaatgggt gcggttggca
      481 ccgtctacgg aagtctgctc tataacaagt atggttcaaa ttttactgtt atatcggatg
      541 aatcaagaaa agacaagttg aagaaaaacg gatttacatt gaatggaaac acattttatc
      601 caaatgtatc atcaaacagc gataatacca ttaaatatga tttaataatt tttgcggtaa
      661 aaaattatca gctagccggt gctcttgaag atgtaagaag tttcgtgggt gaaaacacaa
      721 tattaataac attcttaaac ggggtaactt caagggatat aattcaaggg gcttatccga
      781 ataataaggt tctttatggg ctggcaatga gaatagatgc tgtaagaaat gaagccggag
      841 tagtaaatac tgaagatgga gaaattcatt ttggggaagc ggataataga gtaatttctg
      901 aagaagtgca ggctgtaaag gactgctttg atgctgcagg aatacgaaac attgtatttc
      961 cggatatgat aaggatgtat tggaaaaaat tcatgctcaa tgtgggattt aaccaggtta
     1021 cggcagttac aagggctcct tacggcaagg tgacaaacat tgagacgagt ttaatattgt
     1081 ttaaggaagc aatgaatgaa gttctgacca ttgcgaaagc attaaacatt gatttgaggg
     1141 aagaggatat agaggaattt gttgccttaa tggaccactt ttcacccttt ggcaaaacat
     1201 ccatgctgca ggatgttgag gctaagagaa aaactgaagt tgattatttc ggagggacgg
     1261 ttgttgaatt gggcaagaag cttaatatac ctacgcctgt taaccatgtg ctttattgca
     1321 taattaaatc attagaagaa ttatattaag tattaagaat ataactaact ttatgcggaa
     1381 ctgctaaaac aaacatgggc gactaattga atatcgccct tttttactgt tggaaactta
     1441 ttaataagaa tcagcaaggg gatacataga aagcaggtca tatcccatat caattatcgt
     1501 gatattcatg taatccttat cataaagtaa atcaacatct tcgcctacag tgccgtcatt
     1561 ttttataatt cgcactattg gtcttaatat gttttcggaa tgatttttaa ttactaccgc
     1621 taattcttcg ttgcttagtt taacaaatgt tcctaccgga taggcaaaaa taattttcaa
     1681 aaagtgtttt aaaatattat aatcaaattg tttgtcggca catcccatca aatactctac
     1741 aacttcattt ggaaagatag attttctata tggcctgtct gaaattaaag aatcgtacac
     1801 gtcgcttatt gacaatatcc ttgcgtatag acttatgtta ttgtctcttt tttcatgtgg
     1861 atatcctgtg ccgtcaagtc tttcatgatg gtcttcaact gctcttaaaa cttcatgtgg
     1921 gaccatattt cttaattgct ttgatgcatt gacaggatgt gtctttataa tttcaaactc
     1981 ttcttttgtc agtgttgacg gcttattaag tatttcaatg ggtattaaca ttttaccgat
     2041 atcatgcagc attccggcca aacctaaatc aagaaggtct tgctcgctga gtttcaatga
     2101 tattcccgta gaaatgctta gcatagccac attcaggcaa tgctgaaatg tgtagctgtc
     2161 atagttttta aaatccataa tgttatatga taaatcatct ttattcaata actctattat
     2221 aatgttttta acggtctttg acagccggtt aatcatataa tcgtttattt tccccgaact
     2281 tacttggaat tcataatatg tgtcactaat gcgttgcagt gattctgcct ttaagtcatc
     2341 agcaatatag ggttccacat tgatatcaga aaaggcatcg ttttctatat aaactccatc
     2401 aatattgtga aaattgattc tgttgatgta agtgctgctg agtacttgtc ccttagccaa
     2461 aagaaaagta ttaaaatttc ttcggttata tagataaata tcttttgcca ataccatacc
     2521 cggcttaagg tgatttttag tgatatataa catagaattc tccaagcagt aatattgtat
     2581 agaaatatta attattttct tttttaaacc agtattccat aaataatgct gccatgactc
     2641 caaccatacc tccaaggaca gcaccgatta tagtattaag tgaattgttg ggactggatt
     2701 tttgtgttgg aatagcaggt tgcgaagcta aatatacatt gttttcaaca ataccgataa
     2761 cgctcgtttc aagcttctcg gtccttccgt ttttatagta tttgctgacc gcctgttttt
     2821 caacattcaa ttcctcaatg tactccatgg ttttgttaat agagctctca atagagttta
     2881 tattttgctt gttttgaata atgtcatttt caatttttat ataattagga ttagccgttt
     2941 caataggaat tatataattg ctttcatcgt ttagaggcga ttgaatttca acatttgatt
     3001 tagtatttat tacttttgga gtttgtgaaa gcaattcttc attttttgct aaggtttcct
     3061 tttctacatc caatgtattt tgcaaagcca tcagctcaac actaaaaaga tttaaataat
     3121 ggtttattgc tctttctttt accataacat ccaaaaattc aatatagttt tcaaataagg
     3181 attttgcgag tttatgagat tcctcagggt tatcggcaga aaccgtaaca gtaaagctgt
     3241 tttgtactgt gtttgcctta gtatttgatg ctattacagc aattctttta ttcaagtttt
     3301 caacactgat ttcttcagta tcataattca tgtcctttat tgtgttaata agcacatcat
     3361 cgctgctgat taaattaatg tactgctcac ttgttgtaat aggaagtttg tactccccgt
     3421 atcgggtgtt gtaaatttca ggcatactta ttataatgtc taagcttgtt ttatatacgg
     3481 gcgataatat aaacatacta aatgatgctg caagtattgc aacagccaaa gtaaaggcta
     3541 ttatcatttt ttttcttttc cataaggcca taatcagctc tcttaggtca atttcatcat
     3601 catagtattg tatattattt tgattttcgt tcattatttt actccttatt tgtcttattc
     3661 atcaaacaat atacattata tcagttcaga ctgttctgtt caataagatt taccgatttt
     3721 tacttgaaat tttcaactat tattatgctg tccaattctg ttacaaaatc aaatttttta
     3781 gtttcttcaa gggcagcatc aatatttttt ctttcggttt catgcgtaat aaatatcaag
     3841 ggaacagtat cattgccgtt tttctgtctt tgcactacag aagccagact taccttgttt
     3901 tttccaaaaa tacttgcaat acttccaagg accccaggct tatctataac tttcatgttg
     3961 atataatatt gtttaaaacc tttgttggaa ataacatact ttttctcacc ctcttttgca
     4021 tatgatgagc ctgcattttt aacaatatga attatatctc caagtactgc actacccgta
     4081 ggcagagagc ctgcaccttt gccgtacagc attaattcgc cgactgcatt acctttcaaa
     4141 aatacggcat taaattcatt cttaacggtc gataaagggt gagcggcagg aatgaatgca
     4201 ggttggacat taagctcaat ttcatttcct tttttgattc cggctgccaa cagcttgata
     4261 ttgtatccta attcctttgc atactcaata tcctcttttg atattttcgt aataccttcc
     4321 tttggaatgt ctttaaggtg gattctctgt ccaaaagcta aggtagacag tatagacagt
     4381 ttgtatgccg catcatcacc ttcaacatcg gaagaagggt cagcttctgc aaagccgaga
     4441 gcttgtgctt cctttaaggc ttgtctataa tccagaccgt tttcagtcat ttgcgtgaga
     4501 atataatttg ttgttccgtt aataattcca aaaatatatt caaattcatt tgcacagaga
     4561 ttatctctta ttgtgtttag tattggaatt cctccaccca cgcttgcttc atacattaac
     4621 ttaacattat ttttttctgc caatatatgc agttcttctc cgtaagttgc gataagagct
     4681 ttatttgcgg ttacaacatg ctttttgtta ttgagtgctg cctttatgta ttcgtaagct
     4741 ggacttaatc ctccaattaa ttcaactatt atatctgcat cgctgtttat tacttcatat
     4801 gcatcggttg tgtaaacttc tttgggcaag ttaacatttc ttattttatt ggggttgtta
     4861 acaagaacct tatttaccat taactccccg tttatatttc catgcacttt atctttatta
     4921 aattccataa tgttaataag tcctgatgct actgtcccca tgcctaatat tcctaatttt
     4981 acatgtttta atgattccat tttaatcctc ccttgttccc ggtataaata cacgtgtact
     5041 tttaaaaaac actatatcaa tatagcacaa ttttatatta agttcaagaa aaataatatt
     5101 ccattcatga aagtcttcgg gcaagccata agaatgatac tcaaggggtt acccccggca
     5161 acgcacataa atagcacccg aacttctttt caattcaaag ccgggtgcta tttatttgag
     5221 ttttcttttc cgtatcctgt tggaatcacc tcatttgtca atcatctata ttatcaactt
     5281 tacaaatctt acctctattt tcattcctcg atagactttt actgcacttt gagatattcc
     5341 tatagaattc tccccaaaca atttgattgc agcgacctta taattactca cagatttttc
     5401 gtaagacttt gtattgttgt ataaataata tacccaaaat ttagatgttt aaacagtaaa
     5461 cttttataat acatattatg ctatatataa ttatatgtta ctaattgtga aaaagatgta
     5521 gaatttttgg gaatcatagt tagatataaa aaagttcatt gacacatttt gacaaaattg
     5581 atatactaaa aaaaagtcta tcaatgaaag gagcgggaac atgggtaaat ttgttgttaa
     5641 agaagttaaa acaggtttta aattcgattt aaaggctaca aatggacaag ttattgcaag
     5701 ctctgaaatt tacaagacaa aggcatcgtg tttgaaaggc atagacagcg ttataaataa
     5761 tgcacctatt gctaatttgg aagatcagac tgttgaaaat tttaaagtag aaaagaatcc
     5821 taagtttgaa attttcaccg acaaggcagg agagtacaga ttccgcctaa aagccaagaa
     5881 cggagaaatt atagctacat cggaaggtta taaagccatg gacagctgta aaaacggggt
     5941 agaaagcgtc cgtaaaaacg cagcaaattc aactatagaa tagtaatatg ccataatgtt
     6001 acgaagaaca gcatccgctg ttcttttttt ctaatttctt aaagataaaa ataatactat
     6061 atatgactta ttatatgtta taataacatt gaggttcaaa aaaatacatc gggggaagtt
     6121 tacttgaaaa agtcagtaaa aaaaatattt ttatatgtga gcggcctatt aattcttgca
     6181 atgggagtta atgtttctaa ggctgcacag cttggtatat caccggtcag cgcaattcct
     6241 tatgcattag aacttatatg gaatatagag ctcggcaaag ctgtttttat atttaacatt
     6301 tttttaattg cattgcaaat agctttatta aggaaaaatt tcaagcccat acaattgctg
     6361 cagattgtat gcacattttt attcggtttg tttgttacat ataccggcaa agaattctta
     6421 ttattttggc tgccaatacc atctttgtat atcataaaat tattatattt gtttgtgagt
     6481 atagtaatta ttggtatagg cgtatcattt tatcttattc cggactatat accgcttcct
     6541 gccgaaggaa ctatgaattc aatagtcgaa cttagtaagg gcaagttgaa attttcaaat
     6601 gttaaggttt cggttgacag cagcatggta gctgtttctg ctttactgtc cataattttt
     6661 ttaggacagc tgaaatcagt aagagaagga actatcatag cagcgctctt aatagggaaa
     6721 gttgtaggag ttgtctttaa gcgttataaa ccacaaattg tcgaatggat tgaaaaataa
     6781 tacaaaaaat tttacaaatt tttaacgtgt taaccgcacg ttgtcatcct gagcgtaagc
     6841 gaaggatctc atgagattct tcgagctaaa gctctcagaa tgacaaaaat gttaaccata
     6901 gaaaatatac caaatggata taactatatg ataagaaggt gaaacaaatg aagtatattt
     6961 gttctaaatg caatacaaga atggatgttg caacaaaaaa gtcaaagtgc gactgcggag
     7021 gactttggaa gctggattat aagcccccta agtttgacct ttccttagta gacaaaaata
     7081 attggagtat ttttcgttat cgtgctttca tgcctctttt aggcaatgag tgggaaaaaa
     7141 taagcctggg agaaggtttg acacctgtta ttcaatttaa tgaagatgtt ttattgaaga
     7201 tggattactt catgcccacc ttatcattca aagacagagg ggcagcggtt ttgatagcac
     7261 actgcaaagc catcggagtt gattcggttg ttcaggacag cagcggcaat gcaggaaaca
     7321 gtgttgccgc atattgtgca aaagccggca ttgattgtga aatttttgtg cccgaaggta
     7381 catccccaaa gaaaattgat atgattaaat cccacaatgc aaaggtaaat gtagttcccg
     7441 gatcaagaga caactgtgca aatgtctgca gagcaaagtc agacaaagaa gggaagtatt
     7501 atgcaaatca tgtgtataat cctttctttt atgagggtac caagacctat atttatgaag
     7561 tatatgaaca gcttaataga attcccgaaa atatttttat acctttggga aacggcacct
     7621 tgtttatcgg aataataaag gcattggagg aattcatgga aagcaatatc atagataaaa
     7681 tgcctaatat tattgcagtg caaagcgaat actgcgaccc ctttacaaag gcattcaaaa
     7741 taaaagcttc aaaacccgta aaggtaatat ccaagcctac tatggcagag ggaattgcaa
     7801 taggtgagcc catgagggga gaagaaatat tgaaatatat ctataagtat aatatcgaag
     7861 ttattaattc tcctgaaaca ggaattctgg aagcaagtgc actgcttgct tcaaagggta
     7921 tttatgttga gcatacaaca gcagctacat atgcagcata catgaaatat tgtgaattaa
     7981 aagggagaac tccagctttg attcctatgt gcggagcagg actcaagtca gaccattagc
     8041 gcgttgggac acacctctac taataaggac attctttgat ggcagaggaa tgtcctcaaa
     8101 ttaaaaaaaa atcacacttt attatcgtac gaataaaata gttatagcat ataataaagg
     8161 aagtgatttt ttggcaatca aaatatttat agatcaagga cacaatcctc cgggatttca
     8221 taatgcaggg gccgaaggca acggcttaaa cgaaggtgaa ataaatttcc gtgcaggaat
     8281 ttatcttgga aatttattgg ctaatgatta cagattcgaa tacatgttat caaggcctac
     8341 accaaccacg gtattgggaa cgaacacatc aaccagtctg gcggaaagag ttcgcatggc
     8401 aaatcaatgg ccggcaaatt attttatcag tcttcattgc aatgccaatg ttaatccaag
     8461 tattaacggc tctgaaatgt atatatatca atttaatacc caggctaatt ggctggcaca
     8521 gcatattatg aacggcatag taagaaacac acgcttaagg gataacggga taagggaaaa
     8581 caaatctctt tatgtattaa gaaatacaaa tatgcctgct gtattggttg aaatgggtta
     8641 cttgactaat tttaatgatg caatgatttt aagggataat ttgtggaatg ttgcatacgg
     8701 gatttatcta ggcattttga attattttgg atttttgccg cttaataata taggttagaa
     8761 aaggagagta tattaaacca ccggctcacc ggtggttttg acattttagc ttccttgaac
     8821 tctgcttgta tcgccgaatc ttaaattacg atatataagt actattctat cccaagtata
     8881 tctgtcaacc actcctgtta catctattcc aaagaattcc tgaaatgttc ttaccgcaag
     8941 ctcagtctga ggtccgaaaa ttccatccgg gtcagtcggt gtaattcctt ccaggactga
     9001 agcaatataa gacaaatatt gttgcagtaa gtaaacaccg ggaccctcgg agcctacccg
     9061 atatgtgata cccggccata acaaggcggg aagggcaatc gcggaagggg gaagtgtttc
     9121 taaaattcct gatacagcat tgaacattgt attccaggtc tgctcatcaa caagacctgt
     9181 tattggcaag ttaaatgttt tttggaattc cattagggca gtcctcgttt gggtattcaa
     9241 tacaccgctg atatccacag aaggaataga gttgtagtat gcggacagaa cattgagaaa
     9301 atattgcaca atttgaactc tcggaattac ctcttcagga ccgggagtag gagtgaattc
     9361 gggaatttcg ctcaacataa ttcctcttga agtcaattcc gctaattttg taactgcagc
     9421 atatattctt cttataccgt accatgttga tttgtcaata attcccgtca ccgggaaatt
     9481 aaatatatcc tggaactgtc ttacagcttc ttgcgttgct tcatcaaagg ttcctgtagg
     9541 attaggtatt ctttgtatag caggatagtt tgtggctatt gtgtttaaac gtatctgcat
     9601 caataataca tatggattat tgtctcctaa ttgaagagaa attccgggat aagtttcaaa
     9661 ggcttcagct atcggagtat ctgtttttat atcaatatct tcaccatagt aatattgaag
     9721 catttcatat ggggtgtagc cttgctctgc caattcaacc gtaccccatt ggagaagccc
     9781 cggacaccag gagacgcgac cgtcacaaaa ttgagcataa agaggctgaa tttgtccccg
     9841 tcttattatg taactgtcaa aaagctcatc tgcaagcacc gatacagtgt caaaaatacc
     9901 tctgttatga acaaatgcct ggtcaaatct tgtatcattt gttatatcga aattataacc
     9961 ttgagctcgg taccattcag tgaatatacg gtttaatgcg gtagaagtaa tggcatatat
    10021 atttgctcta agtgcagctt ccggccaagt aggatatagt tcgcttgagg caacattttt
    10081 tatataatca agaaacggaa ccgtaacatt ttcacccggt tcatccggtg cacctaaatg
    10141 aactgtaatt gctgcaggta taaccacttg tgtaagtccg ggaatttcta tactcataat
    10201 atcacctcta atcatttgat atgtcaatcg gactgggagg aataacaatt gttgtatcag
    10261 gaccttctac cggtccgatt gcaacaggaa tcatatttat ttgaaaacgg gtagtggtat
    10321 tcggaaaaat tcttaaatca attacattta ccgtataata gtttattgcc tgaactctta
    10381 aattataagt agtaaaataa tatctgccgc tttcgaaagg gtcaaggcgg tcatacatta
    10441 cgggtaatgt gataagcgga attcgaccgt tggcatcggt tatatgatgc actatttcat
    10501 gttctgcacc actttcatca cggatataaa ccgttactaa ggcaccctct accggcaatg
    10561 ctccggatgc ggtaaaaaca cttaccgata agtagcccac ctcctgctca aaaggtatgg
    10621 agcccggtgg aacttgtgct ttgtaaattc gaccatcgaa tggattatat ttcaaaatat
    10681 ttacacctct ctatctacat aacgaattat attattatat gcatcctaaa atattttgtt
    10741 tatacagttt tggagcggct agattgtagc gtcgggtaat tggattatga ttttgcttga
    10801 aaattttctg taaaaatgtt acaattaaat aaaagcatat cctaaaaatg aggtggctta
    10861 atattgctag ccagagcatt caaattaatt aaaaaccttt tttcaacatc taagacggat
    10921 gattgcaaaa aggaatttgc tattgatttt tgtaggatta atttagctaa gttaataatc
    10981 tctgcaaaaa cacttatcgc attaggaatt attcagctca ttgtatattg tatagtcagt
    11041 aagggaaatc tttttacaaa gccacagata tatttcggag caatgaattt tattttgctg
    11101 tttgcaatga ctatattttt atcggttatt gttaagctgg aaaaggatgc agctaaaaat
    11161 gtggtcagta ttcacaatgc tcaaatattc tttataaact tcatattatt atggagcggt
    11221 gggatatcat tgctgcatca actgacacac ggtcagacaa tagtatatat tgcagcggtt
    11281 ataataaccg caggcgcttt atatatagaa ccctctgttt catttcttat ttattttact
    11341 tcacacttga ttttttccgt actattgcct aattttcaaa agtcgccgga tttgttgttt
    11401 ggaatatatt tgaatactac ttcattggta ataatgtcat ttgctatttc ctatacgaga
    11461 tataagagac aggagcagga ttttaaaaac agaaaaatca tgcaggaaaa cagcgatgaa
    11521 ctgaagcgga ttaataaaga attagaagaa gcgaataaaa agctaaaaat catatcccaa
    11581 tatgatggat taacaggtat tttcaatcga cataccttcg ataccaaaat tagagaagaa
    11641 tggaacagat gtaaaaggca ttcaatgcca ttatcattaa taatggcaga tatagaccat
    11701 tttaaatcat ttaacgataa attcgggcac caggccggtg ataattgttt aaaactggtt
    11761 gcagaaattt tatcggaatg tgcaaagcgc tcttccgatg tagtggccag atatggaggg
    11821 gaagaatttg tgatattgct tcctcaaaca gaaagagaaa gagcatatga tttagctgaa
    11881 aaaatgagaa aaagagtaga agaattaaat ataacaatca gcttaggtgt taatactaca
    11941 atcccatcag atgaatcatc cgttagggaa ttcataaaaa ataccgataa agctctatat
    12001 atagctaaga ataaacgtaa cagcacagaa gtatacttac caattgatac tttcgggttt
    12061 tcttaatgct tgatgcttta atataggcac cttgtatttt tctcccaggg cgcgtacaac
    12121 atccttatag catcctttta atttatcatg aaaaacaatt gcatcaattc cgccaattat
    12181 ggcatctaca cattcagatg ctttttctat tccgttttct ccttcgtatt cgcctgaaac
    12241 aagccattga tttgcaatta tgtgagaaac accgaagaat tcattttcat tccaacatgt
    12301 gtggactgcc tctgcaaaat tcgggtcaaa atgtactcct cccacgcaaa gaagattatg
    12361 cagctttata tcatcaatat ctttgtcaaa tatatgaagc agagaaagag caagaaccct
    12421 gcaggcatcc atatttaccc agctttccgg tgtacttcct atttctatgt ctactatggg
    12481 tacgggatat tttaagagaa gctcagggtc cccctgccct tcataaacac ccgaccaatg
    12541 ggtcgcttca gttactacgc tgaaatccaa ttcgatttct tttcttaatt tctccatagc
    12601 ccacataaga ttgtgcatat ataaaggatt tgcaggaccg taacaacctg aattaacatc
    12661 acctatacta tgtacggtaa taaccttatc aagagcattt gtaccttcgt gccatgtaac
    12721 cattccggac atatcaaaat cagaaaaata cctgttcata tccggaagat acctgggata
    12781 atccctgcat aatgctatat ctgtaggaac aaaatagaat tcattatttt tattatcgat
    12841 atatttatag acttctcttc cgtcaaaggt aaagtcaacc ttattgttta agtatccctc
    12901 ttcctttagt atatcccaaa ccaagtagct gacatatccc caatcatgat tgtcacagat
    12961 ataatatacg gcttttttta cttttgtcat tatgccactc ctataattat tttaattagt
    13021 attataccac ctttttgcac aatgacaagg ggacgtgaca aggggacggg ggtattgtca
    13081 tctttttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aagaaaattt
    13201 atgaaggctt ccggaaatcc aaatcagtgg aaggatagtt accctgagag aacattgatt
    13261 gaaagtgata ttgaaaataa tataagttac gtgtgtgttg atgaaggtag aattgtcgga
    13321 acatttgtat attttaaagg tatcgagcct gattatgcag aaatacacga cggccagtgg
    13381 cttaatgaag agccttacgg agttgttcac aggattgctt cagcaaccgg caaacggggt
    13441 gttgctactt attgtttgaa ttggagcttt gagcaatgcc gtaatttaaa gatagatacc
    13501 catcatgaca atattcccat gcataattta ttaactaaaa acggttttaa tcgctgcgga
    13561 attatttatt tagaaaatgg tgatgaaaga atagcatacc aaaaagctga cttagcataa
    13621 tgaataaaag atagaaaaaa atatttaaaa aaaacacttg acaaaagcct ttatatagac
    13681 tacattagat aacaattaaa taaattctca tcaagagaga tggagggaac ggcccgatga
    13741 aatctcggca acctgcattt actaatgcaa ggtgctaatt ccggcaaagg tatctttgaa
    13801 agatgaggag acgaaacaat aaattttcct ctcttcattg gaggattttt tttattgact
    13861 cgacccaatt tgtcaaattg gtcatgaggt ctgcgaattt taagcaaata taaaggagga
    13921 aacatgaaaa acaattttaa attagaaaca gccttggtac aatcgctgga agaatttcaa
    13981 gaaggagaag caagagtcta tcctttggtg caaagcacta catataatta caaggaccct
    14041 gattatgtgg cagctttatt tgatttaacc gagtccggac acatgtattc aagaataagc
    14101 aacccaaccg tagaggcttt tgaaaataaa atggcacatc ttgaaggcgg agtaggagca
    14161 gttgccgtat cttcaggcat ggcagcaaca acaatggcca taaccaacat atgcaattcg
    14221 ggagaccatg taatagctct atcgactatt tacggcggga catttacatt gctctcatca
    14281 agcttaaaaa agttcggtat agaaacaaca ttttttggtc ctgacagcac tgaagaagaa
    14341 atactgtcct tggttcaaga caatactaaa ttaatattcg gcgaaacaat aggcaatccg
    14401 ggaatgaatg tattggattt taaaacagtt tccaatgcag cccaaaaagc gggccttccc
    14461 tttattgttg ataatacaat tgcaactcct tatctatgca gaccgataga
//
LOCUS       DAOPUE010000043        14472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4270_length_14472_cov_9.586183, whole genome shotgun sequence.
ACCESSION   DAOPUE010000043 DAOPUE010000000
VERSION     DAOPUE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_14472_cov_9.586183"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1073
                     /gene="ispG"
                     /locus_tag="PKH42_05405"
                     /gene_synonym="gcpE"
     CDS             12..1073
                     /gene="ispG"
                     /locus_tag="PKH42_05405"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOA19901.1"
                     /translation="MDKIIRRQSSKVMVGNVQIGDGADITVQSMTNTFTKDVKSTVAQ
                     ILQLEEAGCEIIRVAVADNEDAEAIKEIKKQIHIPIVADIHFDYRLALKSIESGIDKL
                     RINPGNIGGIDRVKKVVEKAKPRNIPIRIGVNVGSIHKEILKKYNAPTSEGMVESALE
                     HVRILEDLDYGNIVISMKGTDIKMTIDAYKSMAKKVNYPLHLGITHAGTLFEGSIRSA
                     IGVGSLLADGIGDTIRISLTDDPREEVKVAREILKSLNLRNFGINYITCPTCGRTKIQ
                     LIELSKKIQEGLKNVNKPITVAIMGCAVNGPGEARQADIGIAGGKGEALLFKKGEIIE
                     KVKEEDLVKRLLEEIDKLS"
     gene            1195..1803
                     /locus_tag="PKH42_05410"
     CDS             1195..1803
                     /locus_tag="PKH42_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOA19902.1"
                     /translation="MAVGTLNVEKRLETNSRASNRLRRQGYLPGNISRKGKDSVSVTV
                     KADELRKGLSTYGRNALFKITLDDTEFTGMVKDIQLSPVKGSMMHVDFQEVSLNEEIK
                     VQLSIVLKGTEALEFKGLIALTQLDSITVKGLPQNIPDDLTIDVSNIEKVENICLKDV
                     KFTEGIVPEGHPEQVLISIVEAKRTVEEEETGEETDDEVAGE"
     gene            complement(1855..2475)
                     /locus_tag="PKH42_05415"
     CDS             complement(1855..2475)
                     /locus_tag="PKH42_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOA19903.1"
                     /translation="MICGGIIGLERSRKNRPAGFRTYMIVCLSSALVMMTNQYIYNNF
                     SGSDPARLGAQVISGIGFLGAGTIMITSRNQIKGLTSAAGLWASACLGLAIGVGFYSG
                     AVIVALIVFIIITFFKKIDYMLTSNNRIINIYTSFKSIEQLDKFIIFCNLSDFKVRNI
                     EITKDTIPHEISVIAIITLEAKERMNHVDVIQKLNSFEGLVHIEEL"
     gene            2677..6915
                     /locus_tag="PKH42_05420"
     CDS             2677..6915
                     /locus_tag="PKH42_05420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOA19904.1"
                     /translation="MLFNKIFTDESLDKIKIQKIEVIERTNTINLHALSQEIIDFNHI
                     ENIKDEISKHYNNSVNVEIYLKYKLEKNINIEKIKENLLYIINKSSNILSKAKDNIKI
                     EFKDECFFVIADNKLLIDELNDNNTERKLKKLICDYNLDYNIKAIYNEAEEEAAITAQ
                     RIFDQALKKNLEIIKNNSSEKKPANKSQEGSYKKRSRKKIDFDSIPVSLISELTNDSG
                     VVNIKGKIVNVDKRELKNGSFLISLIITDSTDSIMCKYFCPNGFDDEGIENGNYIQIA
                     GNVEYDDYEKELIVKILDLQLTTEEIKRMDNAKEKRVELHLHTGMSSMDGINSFKEYA
                     KKARQWGHKAIAITDHGVVQGYPEAMDCADDNFKVIYGMEGYLVNDTVSIVQNCQNVN
                     LDSVFVVFDIETTGLNPKNDNIIEIGAVKISNRKVIDSFSTFVHIDKKLPGKIIELTS
                     ITDDMLQGQPEIDEALPAFLDFAKECVLVAHNAKFDLGFIKENTKVLSIENYNPPVLD
                     TLELSKALIKDVKNHRLNTLTKKLGINLINHHRAVDDANATGQLLIILLNKLSEREIY
                     DVSKLNDILMEDIDIVKQPSYHINILVTNYIGLKNLYSLVSDSHINNFYKKPRLLKSN
                     LNQLREGLLLGTACESGELFSAFLEGKSDEKIEEIADYYDFYEIQPLDNNMFLVNNER
                     LRSVEELKIINKKIVELGERKGKTVVATGDVHFLEPEDEIFRRILMSGQGYEDAEEQA
                     PLYFKTTDEMLDDFSYLGEEKAYEVVVTNTNLIADLIEPILPIPNGTFPPIMEGSDNE
                     LRNICFSKAKSIYGEKLPEIVESRLNRELNSIIGNGYSVMYMIAQKLVKKSNEDGYLV
                     GSRGSVGSSFAATMAGITEVNPLVPHYVCNKCKYSEFIEDGSYGSGFDLPDKHCPNCG
                     TLLVKDGHDIPFEVFLGFEGDKEPDIDLNFAGEEQGICMKYTEELFGEGKVFRAGTIS
                     TIADKTAYGFVKNYFEERGITKRKAEIERLIQGCLGIKRTSGQHPGGVMIVPKDKDIH
                     DFTPVQYPANNEKSGTITTHFDYHSISGRILKLDLLGHDTPSIIRMLEDLTGLESKDI
                     PMDDPSTMAIFNSPEILGISLDEINCSTGTLAIPEFGTSFVRQMIKDTKPKSFSDLVR
                     ISGLSHGTDVWINNAQDIIKKGYAKIGGVISTRDDIMTYLIQMGVEKKTAFDIMERVR
                     KGKGLRKEDEEAMKAQNVPQWYIDSCNTIKYMFPKAHAVAYVTMSVKIAYYKVHYPEA
                     FYATYFTTKVDDFDADIVMKGQNHILNTIKSFEEKGNDKTAKEKNLITVLEVAYEMYK
                     RGFKFTKVDLYKSDDRKFLLTEDGILPPLMGLQGLGANAAANIKAEREISEFISIEDI
                     INRAKVSKTVIEVLEKHGCLKNMDETNQISIFNV"
     gene            7198..7662
                     /gene="rimP"
                     /locus_tag="PKH42_05425"
     CDS             7198..7662
                     /gene="rimP"
                     /locus_tag="PKH42_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOA19905.1"
                     /translation="MNKKDIVSSVRKITEEILKSTDIELFDIEYVKEGPFKYLKVYID
                     KPGGITVDDTADISRVLNKKLDEVDLIKEQYFLEVSSPGVERPFKTEKDYLKNLNKLV
                     EARLYKPIDDKKTVKGILTEKNENNIVIKSGENNFTIELKDLAKINRVIEDF"
     gene            7675..8733
                     /gene="nusA"
                     /locus_tag="PKH42_05430"
     CDS             7675..8733
                     /gene="nusA"
                     /locus_tag="PKH42_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOA19906.1"
                     /translation="MNKDILNALSELEREKGILKDVVLEAIRSALETGYKKNYGKATN
                     FSIEIDKTTGEYKLFQDKTVVEKVENKNLEISLEEAKSIDSKYELGDIVKIQIEPKED
                     FGRIAAQTARQVILQKIREAERESIFSEFYGRESDILTGIVQRENKGNIFVDLGKIEG
                     ILSVNEQIPGEVYNPGDRIKTVILEVKNTGKGANIMLSRSHPNLVKRLFELEVPEIHD
                     GIIEIYSIAREAGSRTKIAVFSHDPNVEPIGSCVGNKGTRVKNIIDELNQEKIDIIIY
                     EKDPEKFIANSLGPAKILKVTANEKEKTAVTVVPDNQLSLAIGKEGQNVRLAAKLTGW
                     KIDIISESQYNDSLREEV"
     gene            8730..8999
                     /locus_tag="PKH42_05435"
     CDS             8730..8999
                     /locus_tag="PKH42_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOA19907.1"
                     /translation="MKVRKIPMRRCIGCMESFPKKELCRIVKTKENEIFFDETGKKNG
                     RGTYICKNIECFNKAIKSKRLAKSLETEIPEEVIEAIKEAIEKNA"
     gene            8992..9285
                     /locus_tag="PKH42_05440"
     CDS             8992..9285
                     /locus_tag="PKH42_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOA19908.1"
                     /translation="MHKIYSMLGIARKGGNVSIGFDSTKADIEKNNSFLVIIAEDASD
                     KTKKNIKFVCDKYNCEYIETGEKELLGKALGKKVVSVLSIRDRNIASYLLNNV"
     gene            9301..11499
                     /gene="infB"
                     /locus_tag="PKH42_05445"
     CDS             9301..11499
                     /gene="infB"
                     /locus_tag="PKH42_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOA19909.1"
                     /translation="MRIYELAKELEMNSKDLAQLVEQELGITVKNHMSSLTDLEVLRF
                     KKALSLLNNSKKTKLVKSSKNVIVDEEPLVDEKIDLNQAKLKKVDKKLLSPVKKHTPT
                     KIGPAKLHHDEEEKKEIRKDIKKDKQKQKPQQQQQSRPEPKQHKVKNISIEDSIVVRD
                     FADKLGVPVNVVISKLIMLGVMANMNQEIDYDTASLIGEELGAKVNKIEVVDELKNLE
                     DSLFKDDKDDESNLQPRPPIVTVMGHVDHGKTSLLDAIRNTSVTEHEAGGITQHIGAS
                     QVEINGKKITFLDTPGHEAFTAMRARGAKVTDIAIIVVAADDGVMPQTIEAISHSKAA
                     GVPIIVAVNKIDKPTANPDKVKQELSEHGVISEDWGGDTIFVHVSAKKGTGIEELLEM
                     ILLVAEVQELKANPNRNAKGTIIESKLDKGRGPVATVLIEKGTLKKGDYVLVGSTHGK
                     VRAMFNSKGKRINQAGPSVPVEILGLSETPQAGDILVVMENEKDAKNIAEKRKDKKHL
                     ESINVSSKVSLDDLYERIQKGEVKDLNIIIKADVSGSIEAVKQSLQKLSVDEVKVNPI
                     HGGVGGINENDILLASASNAIVIGFNVRPSVAALELAKQENVDVRTYNIIYNAIEDIE
                     SAVKGMLKPVFKEVVMGRAEVRQTFKVPNVGIVAGVYVTSGKITRKSRIRLLRNDIVI
                     HDGQIASLKRFKDDVSELSSGYEGGIGLDKYNDIKIGDSMEAYIMEEIKR"
     gene            11513..11890
                     /gene="rbfA"
                     /locus_tag="PKH42_05450"
     CDS             11513..11890
                     /gene="rbfA"
                     /locus_tag="PKH42_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOA19910.1"
                     /translation="MGNRRNNRLSGEVRKAISEIIINDVKDPRISELMSITDVHVTED
                     LKYAKVFVSDYNNIETTLTALESAKGFIRKELGKKVKMRIIPELIFIKDDSIERGLHM
                     SSLIDKVIEEDKTRKVNKDNAEE"
     gene            11841..12839
                     /locus_tag="PKH42_05455"
     CDS             11841..12839
                     /locus_tag="PKH42_05455"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOA19911.1"
                     /translation="MKKIKQERLIKIMLKNDVKEIFNLINNSDKICIAGHKAPDGDCI
                     GSVMALYNFLKPLNKNITVLMDGNIPYNYKAFIDEKIISNSYNNEEYDLLIVLDTSDR
                     KRLGKFENVLNNSKKTVVIDHHKTNEGFGNINIIDTEMSSTGELVYDILKAADADISL
                     EVAALIYLAILTDTGKFSYRNTTSNTHRKAAELIDIGVNVSEIDNIVYNSKPSNIVRA
                     FMECVSGVELYYDNKLGITAITKNILNKHNADMADIDGVVEYIREIKEVEVSCVLKEN
                     DEGTKVSLRSKNNIDVAAVSVKYKGGGHAKAAGFDINENIESTKKIIIEEFKKYFA"
     gene            12839..13708
                     /gene="truB"
                     /locus_tag="PKH42_05460"
     CDS             12839..13708
                     /gene="truB"
                     /locus_tag="PKH42_05460"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOA19912.1"
                     /translation="MIGILNILKPTGMTSHDVVSNVRKILNIKKVGHAGTLDPNVAGV
                     LVICVGKATKFSEYLMSGDKEYVCELILGQSTDTQDSYGMVIDKSDKVSVTREELNEV
                     LKKFTGELIQIPPAYSAVKLHGKKLYEYARQNIQVEKQGRPINIKSIELLKFDGNKAL
                     LKVRCSKGTYIRTLCNDIGKSLGTFGHMGILIRTESKGLHINNSVTLEKLKYLNDSNK
                     LDECLIPVESIYKLDKINVESKYYDRLTAGNEVEAGLNTDSDKFYVYCRNSLIGIGKK
                     VKDNTIKIDKMLR"
     gene            13721..>14472
                     /locus_tag="PKH42_05465"
     CDS             13721..>14472
                     /locus_tag="PKH42_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HOA19913.1"
                     /translation="MIKTIGSCIAIGNFDGIHVGHDVLIRRMIDLSKERNLKSIIITF
                     KYVRNDLKKSSMNLKYINSPETKLEILKSYNVDEVIEIELNEIISKYSPEQFIKLLVD
                     DYNAKNIVVGYNFTFGHKASGNINTLKEFQDKYGYRVEEIPPVKYNGIAVSSSLVRTL
                     LNEGKINEANTLLLNKYTIYNDELNINYNKNIGFVDNKSSILVPPDGKYIVKLGSKEY
                     ILNIVSNKSGAALIFDKEVEENKDIVFINKAS"
BASE COUNT         5612 a         1976 c         2922 g         3962 t
ORIGIN      
        1 gatagaaaag tatggataaa ataatcagaa gacaaagctc aaaagttatg gtcggtaacg
       61 tacaaatagg tgacggtgca gatataacgg tacaatccat gactaatact tttacaaaag
      121 atgtaaagtc aacggttgct caaattctgc agcttgaaga agcaggctgc gaaattataa
      181 gggtggcggt tgcagataat gaagatgctg aagcaattaa agaaataaaa aaacaaattc
      241 atataccgat tgttgctgat atacattttg actatagatt agcattaaaa agcattgaga
      301 gcggaattga taaattgcga attaatcccg gtaatatagg cggcattgac agggttaaaa
      361 aggttgtaga aaaggcaaaa cctagaaata ttccaatcag aataggcgta aatgtaggaa
      421 gtatacataa ggaaatatta aaaaagtaca atgctcccac ttcagaagga atggttgaat
      481 ctgctttgga gcatgttcgt atattagagg accttgatta cggcaacatt gtaatctcta
      541 tgaagggaac agatataaaa atgaccattg acgcctacaa atctatggcg aaaaaggtta
      601 attatcctct tcatcttgga attactcatg caggaacgtt atttgaaggc agcatacgct
      661 ccgccattgg tgttggaagt cttttggctg atggtattgg cgacacaata agaatatctc
      721 taacagatga cccgagggaa gaagtaaagg ttgctagaga aattttaaaa agtcttaatt
      781 taagaaattt cggaataaat tatataacat gtccaacatg cggtcgaact aaaatccagc
      841 ttattgagct atccaaaaaa attcaggaag gtttaaagaa tgttaataaa cctataacag
      901 ttgcaataat gggctgcgca gttaacggac cgggagaggc aagacaggcg gatatcggta
      961 tagcaggagg taaaggagag gccctgttgt ttaaaaaggg ggaaataata gaaaaggtaa
     1021 aagaagaaga tttagttaaa aggctgctgg aagaaattga taaactctcc taaggttgac
     1081 attcaaacaa aaggtgtagt ataatttata tatgaaaatt caatattttt taattaacct
     1141 tatcgctgta taaaccaaag cgtattaaat aaattgcata tctaaggagg aaacatggca
     1201 gtaggaactt taaatgtaga aaagagatta gaaacaaaca gcagagcaag caacagattg
     1261 cgaagacaag gatatttgcc cggaaacata tccagaaaag gtaaggattc ggtttcagtt
     1321 accgttaaag ctgatgaact gagaaaaggc ttatcaactt acggaaggaa tgctttgttt
     1381 aaaataacat tggatgacac ggaattcacc ggtatggtga aagatattca actttcacct
     1441 gttaagggct caatgatgca tgttgacttt caagaagtat cattaaatga agaaatcaaa
     1501 gttcaattat caattgtgct taaaggtaca gaagcattag aatttaaagg attaatagca
     1561 ttaacacaac tagattcaat cacagtgaaa ggtttgccgc agaatatccc tgatgaccta
     1621 acaattgatg tttcaaatat cgaaaaagta gaaaatatat gtttaaaaga tgtaaaattt
     1681 actgaaggaa tagttcctga aggtcaccct gaacaagttt taatctctat tgttgaagct
     1741 aagagaacag tagaggaaga agaaaccggc gaagaaaccg atgatgaagt agcaggtgaa
     1801 taacaagatt aataatagta ttaaataaaa atacccaatt tacgggtatt ttttttacaa
     1861 ttcttctata tgaacaagtc cttcaaagga gtttagcttt tgtattacat caacgtgatt
     1921 cattctttct tttgcttcca gtgtaattat tgcaataacc gatatttcgt ggggtatagt
     1981 gtcttttgta atttcaatgt tgcgtacttt gaaatcggat aaattgcaaa aaattatgaa
     2041 tttatccaat tgctcaatgg atttaaacga agtgtatata ttgataattc tattgtttga
     2101 tgttagcata taatcaattt ttttaaaaaa ggttattata ataaaaacaa tcaatgctac
     2161 aattactgca cctgaataaa accctacacc tattgcaagg cctaagcatg cagatgccca
     2221 taaacctgca gctgaagtaa gtccttttat ttggtttctg cttgttatca ttattgttcc
     2281 tgcacccaaa aaaccgatac cgctgataac ctgtgcacca agtctagcag ggtcactgcc
     2341 gctgaaatta ttgtaaatat attgatttgt catcattacc aaagcagagc ttaaacatac
     2401 aatcatataa gttctgaaac ctgcaggtct gtttttcctg cttctttcaa gtcctataat
     2461 acctccgcaa atcatagaaa gtacaattcg caatgttatg gaatatatat tgagttcata
     2521 tagcgtttcg attggaattc ctcctttgta tttattaata tattaataaa tgtaaaattt
     2581 attacatatg tttactttta aaatatattt taagtatatt gaattaaaat tttaattttg
     2641 ttatactaca ataggagaaa ataaagggag actgctatgt tatttaataa aatatttact
     2701 gatgagagcc ttgataagat aaaaatccaa aagattgaag taatagaaag aacaaatact
     2761 attaatttac acgcgttaag ccaagaaatt atagatttta atcatataga aaatattaag
     2821 gacgaaataa gtaagcatta taacaattca gtaaatgtag aaatatattt aaaatataaa
     2881 ttagaaaaaa atataaacat tgaaaaaatt aaagaaaacc tgctttacat catcaataaa
     2941 agttcaaata tactgtcaaa agcaaaggac aatataaaaa ttgaatttaa agatgaatgt
     3001 ttttttgtaa ttgcagacaa taaactgctg atagacgagc ttaatgacaa taacacagaa
     3061 agaaagctta agaaacttat atgcgattac aatttagact ataatataaa agctatatat
     3121 aatgaggcgg aagaagaagc agcaattact gcacaaagaa tttttgacca agcattgaaa
     3181 aagaatttag aaataattaa aaataactct tccgaaaaaa aacctgccaa caaatctcaa
     3241 gaaggcagtt ataagaaaag gtcaagaaaa aagattgact ttgacagcat acctgtgtct
     3301 ttaatttccg aattaacaaa tgactcaggt gtagttaata ttaaaggaaa aattgtaaat
     3361 gttgacaaaa gggagttaaa aaacggctct ttcctcatat ccttaatcat aaccgattcc
     3421 acggattcaa taatgtgcaa atatttttgt cctaatgggt ttgatgatga aggaattgaa
     3481 aatgggaatt atattcaaat tgcaggcaat gttgaatatg atgattatga aaaagaattg
     3541 attgttaaaa tactagattt acagttaaca accgaagaaa tcaagcgaat ggataatgca
     3601 aaagaaaaga gggttgagct tcatctgcat acaggtatga gctcaatgga tggtataaac
     3661 agctttaagg aatacgctaa gaaggcaagg cagtggggtc ataaggcaat tgcaataaca
     3721 gaccacggtg ttgtgcaagg ctatcctgaa gcgatggatt gtgccgatga taattttaaa
     3781 gtcatatacg gtatggaagg ataccttgta aatgacactg tctctatagt tcaaaattgt
     3841 caaaatgtta atttggattc tgtatttgtt gtatttgata tagaaactac aggtttaaat
     3901 ccaaaaaacg acaacataat agaaatcgga gcagtgaaaa tttcaaacag aaaggttatc
     3961 gacagctttt caacatttgt acacattgat aaaaagcttc ctggaaaaat cattgaatta
     4021 acatcaataa cagatgatat gcttcaaggt caacccgaaa tagatgaggc tttgccggca
     4081 tttttggatt ttgcaaaaga atgtgtattg gttgctcaca atgctaaatt tgaccttggt
     4141 tttattaaag aaaacactaa ggtactgtct atagaaaatt ataatcctcc ggtcttggat
     4201 actttggagc tctcaaaagc tctgataaag gatgttaaaa accatagact taatacttta
     4261 acgaaaaaat taggcataaa tcttataaac caccacagag cagttgatga tgcaaatgca
     4321 acgggacagc ttttgataat tttattgaac aagttaagtg aaagagaaat atatgatgtt
     4381 tctaaattaa atgatatcct tatggaagac atcgatatcg ttaaacagcc gtcatatcat
     4441 atcaatatac ttgttaccaa ctacatagga cttaaaaatt tatacagttt ggtttcagat
     4501 tcacatataa ataattttta caagaaacca agactgttaa aatctaactt aaaccaatta
     4561 agagaaggtc tattgctcgg aactgcttgt gaatcaggag aacttttcag tgcctttctt
     4621 gaaggcaaga gtgatgaaaa gattgaagaa atagctgatt attatgattt ttatgaaata
     4681 cagccgcttg acaacaatat gtttttagta aacaatgaaa gattaagaag tgttgaagag
     4741 cttaaaataa tcaacaaaaa aattgttgag ttaggtgaaa ggaaaggcaa gacagtcgtt
     4801 gcaaccggtg atgttcactt tttggagcct gaagatgaaa ttttcagaag aattctaatg
     4861 tcaggacaag gctatgagga tgcggaagaa caagcccctc tatattttaa aacaaccgat
     4921 gaaatgcttg atgatttttc atatttaggt gaagaaaagg catacgaagt ggttgtaaca
     4981 aacacaaatt taattgcaga tttaatcgag ccaatacttc ccattccaaa tggaacattt
     5041 cctccaataa tggaaggctc ggacaatgag ttaagaaata tatgttttag caaagctaaa
     5101 tcaatatacg gagaaaaact ccccgaaatt gttgaaagca gattaaacag ggagttaaat
     5161 tcaataatcg gtaacggata ctcggttatg tatatgattg ctcaaaaatt ggttaagaaa
     5221 tcaaatgagg acggctactt ggtaggctca agaggctcgg taggctcctc ctttgcagca
     5281 acgatggcag gaataacgga agtaaacccc ttagttcctc attatgtatg caacaaatgc
     5341 aagtattccg agtttataga agacggaagt tacggctccg gttttgattt acctgacaag
     5401 cattgcccca attgcggcac tttattggta aaagacggtc atgacatacc ctttgaggta
     5461 ttcttaggat ttgaaggaga taaagagcct gatatagatt taaattttgc cggtgaggaa
     5521 caaggtatat gcatgaaata taccgaagag ctttttggag aaggaaaagt tttcagagca
     5581 ggcaccataa gtacaattgc agacaaaaca gcctacggct ttgtgaagaa ttattttgaa
     5641 gaaagaggca ttactaagag aaaagcagaa attgaaaggt taattcaagg atgtctgggt
     5701 attaagcgaa cttcaggaca gcatcccggc ggggttatga tagttccaaa agataaggat
     5761 attcatgatt ttacgcctgt tcaatatcca gcaaacaatg aaaagtcggg aacgataaca
     5821 acacattttg actaccattc catttcggga cgaattttaa agcttgattt acttggtcat
     5881 gatactccaa gtattatacg catgctggag gatttaacag gactggaaag caaagatatt
     5941 cctatggatg acccaagcac aatggccatt tttaacagtc ctgaaatact tggaatttca
     6001 ttggatgaaa taaattgcag cacgggaaca ttggcaatac ctgaattcgg tacttctttc
     6061 gtaaggcaga tgattaagga tacaaagcca aaatcatttt ctgacttggt cagaatcagc
     6121 ggtctttcac acgggacgga tgtctggatt aacaatgcgc aggatataat aaaaaaaggt
     6181 tatgcgaaaa tcgggggagt tatatctact cgtgacgata taatgacata tttaattcaa
     6241 atgggagtag aaaagaagac ggcttttgac ataatggaaa gagttcgaaa aggaaaagga
     6301 cttcgaaaag aagatgagga ggcgatgaag gcacagaatg ttcctcagtg gtacatagac
     6361 tcctgcaata caattaaata tatgtttccc aaggcacatg cggttgctta tgtaaccatg
     6421 tcagtaaaaa ttgcttacta taaagttcat tatccggaag ctttttatgc aacatatttc
     6481 accaccaagg ttgatgactt tgatgcagat attgtaatga aaggtcaaaa ccatatattg
     6541 aatacaatta aatcttttga agaaaaagga aacgataaga cagcaaagga aaaaaattta
     6601 attactgttc ttgaagtagc atatgaaatg tacaagcgag gtttcaaatt tacaaaagtt
     6661 gatttatata aatccgacga cagaaaattt cttctgaccg aggacggtat actgccacca
     6721 ttgatgggtc ttcaaggtct tggggcaaat gcggcggcaa acataaaggc tgagagagaa
     6781 atttccgaat ttatttcaat tgaagatatt ataaatagag caaaagtatc aaagactgta
     6841 atagaggttt tggaaaaaca cggctgcctg aaaaatatgg atgaaacgaa ccagatttct
     6901 atttttaatg tgtgattgat ttgtcctgtc tttataactg agtgctcaat aattcaagta
     6961 atgtgtgttt ttcgttgtat gtatttccat acataaggtg cacctttttc atagatattt
     7021 tcaaacaata cttgaaattt tgagcggagc gagcaaaaaa gcactatttt tcattgtgct
     7081 atttataaaa taatgtttga ttttataaaa tatttgtgat atactattaa aaagaagaat
     7141 tttgacagaa agagtgggtg accccactct ttctgattgc agggtaaagg agtaaatatg
     7201 aacaagaaag atatcgtatc ttcagtcaga aaaattacag aagaaatact aaaatctacg
     7261 gatattgaat tatttgatat cgagtatgtt aaagaggggc cctttaagta tttaaaagtc
     7321 tacatagata agcccggggg aattactgtg gatgataccg ctgatataag cagagtactc
     7381 aataagaagc ttgatgaggt tgatttaatc aaggagcaat attttttgga agtttcttca
     7441 ccgggagtgg aaagaccttt caaaacagaa aaagactatt tgaaaaactt aaataaatta
     7501 gtggaagcaa gattatataa accaattgat gacaagaaga ctgtaaaagg catattaacg
     7561 gaaaagaacg agaataatat tgtaatcaaa tcaggagaaa ataattttac gattgaactg
     7621 aaagatttag caaaaattaa tagagtgata gaggattttt aatggaggcg ggttatgaac
     7681 aaggatattt taaatgcatt gagcgagctt gaaagagaaa aaggtatttt gaaagacgtt
     7741 gttttggaag ctataaggtc tgctttggaa acaggatata agaagaatta cggtaaggca
     7801 acaaacttca gcattgaaat agataaaaca acaggtgaat ataaactttt tcaggacaaa
     7861 accgttgtag aaaaagtaga aaataaaaat ttggaaattt cattggaaga ggcaaaatca
     7921 atcgattcaa aatatgaatt gggagatata gtcaaaatac aaattgagcc caaagaggat
     7981 ttcggtcgaa ttgcagctca aacagcaaga caggtaattt tgcaaaaaat ccgcgaagct
     8041 gaaagagaat ctattttcag tgaattttac ggcagggaat cagatatttt gacaggaata
     8101 gtacaaagag aaaacaaggg caacatattt gtggatttag gcaaaattga aggaatactt
     8161 tccgtaaatg agcaaatacc gggcgaagta tataatccgg gtgacagaat aaagactgtt
     8221 attctggagg taaaaaatac gggaaaaggt gcaaacatta tgttgtcaag aagccatcca
     8281 aatcttgtca agaggttatt tgagcttgaa gtacctgaaa ttcatgatgg cattattgaa
     8341 atatattcaa ttgcaagaga agccggctca agaacaaaaa tagcagtttt ttcccatgac
     8401 cccaatgttg agcccatagg ctcctgtgta ggaaataaag gtacaagggt taaaaatatt
     8461 attgatgaat taaatcagga aaaaattgat attattattt atgaaaaaga tcccgaaaaa
     8521 tttattgcca atagtctggg acctgccaaa attcttaaag ttacagcaaa tgaaaaggaa
     8581 aaaacggcag tgactgttgt tcccgacaat caactttctt tggcaattgg aaaggaagga
     8641 caaaatgtaa ggcttgctgc aaagcttaca gggtggaaaa ttgatataat aagcgaatct
     8701 cagtataacg acagcttacg ggaggaagta tgaaagtcag gaaaatacca atgagaagat
     8761 gtatcggctg catggaaagt ttccccaaaa aagaattatg cagaattgta aaaacaaaag
     8821 aaaatgaaat ttttttcgat gaaacaggaa agaagaacgg acgaggaact tatatatgca
     8881 aaaatataga atgcttcaat aaggcaatta aatcaaagag gttggcaaaa tcattggaaa
     8941 ccgaaattcc tgaagaagtg atcgaagcaa taaaagaggc gattgaaaaa aatgcataaa
     9001 atatactcaa tgttaggtat tgcaagaaaa ggaggaaatg tatccatagg ttttgattca
     9061 acgaaggcgg atatagaaaa aaacaatagt tttttggtta taattgcaga agatgcatca
     9121 gataaaacta agaaaaacat taaattcgta tgtgataagt ataattgcga atatattgag
     9181 acgggtgaaa aagaactatt gggaaaggct cttggaaaaa aggtggtgag tgttctgtca
     9241 ataagagaca ggaacatagc atcgtattta ttaaataatg tataaaccgg aggtggttgc
     9301 atgagaatat atgaacttgc aaaagaatta gaaatgaaca gcaaagatct tgcacaatta
     9361 gttgaacagg aattaggaat tacagtgaaa aatcacatga gctccctgac tgatttagag
     9421 gttttaagat ttaaaaaggc attaagcctt cttaataaca gtaaaaaaac aaagcttgtc
     9481 aaaagcagca aaaatgttat cgtagatgaa gagcccttgg ttgacgaaaa aatagatttg
     9541 aatcaagcaa agcttaaaaa ggttgataaa aaattgttat caccggttaa aaagcatact
     9601 ccgactaaga ttggacctgc aaaattacat catgatgaag aagagaaaaa agagattagg
     9661 aaggatataa aaaaggataa gcaaaaacag aaaccacagc agcagcagca aagccgacct
     9721 gagccgaaac aacacaaggt taaaaatatc agcattgagg attctattgt agtaagagat
     9781 tttgcagaca agttgggtgt tcccgtaaat gttgtaatat ccaagttgat tatgctcggt
     9841 gtaatggcaa atatgaatca ggaaatcgac tatgacaccg catcattgat tggagaagaa
     9901 ttaggcgcaa aagtaaataa aatagaagtt gttgatgagc ttaaaaattt agaggattca
     9961 ttatttaaag atgataaaga tgatgaaagt aacttgcagc cgagaccacc tatagttact
    10021 gtaatgggtc atgttgacca tggaaaaact tcgcttcttg atgcaatcag gaatacatct
    10081 gttacagagc atgaagccgg aggaataact cagcatatag gagcgtcaca ggttgaaatt
    10141 aacggcaaaa aaataacatt ccttgatact cccggacatg aagcatttac cgcaatgagg
    10201 gcaaggggag caaaagtcac tgatatagcg ataattgtag tagctgcaga tgacggtgta
    10261 atgcctcaaa caattgaagc aataagccat tcaaaagctg caggagttcc tataattgta
    10321 gccgtaaata aaatcgataa acctactgca aacccggata aagtaaagca ggagctgtca
    10381 gagcacggtg ttatatccga ggattggggc ggagatacta tatttgtgca tgtttcggca
    10441 aaaaaaggca ccggtataga agaactgttg gaaatgatac tattggttgc agaagttcag
    10501 gaattaaaag ctaatccaaa cagaaacgca aagggtacaa ttattgaatc aaagcttgat
    10561 aaaggaagag gtcctgttgc taccgtattg attgaaaagg gaacattgaa aaaaggcgat
    10621 tatgtattgg taggaagcac tcatggcaag gtcagagcca tgttcaattc aaaaggcaaa
    10681 agaattaatc aggcgggacc gtctgttccg gttgaaatat taggactttc tgaaacgcct
    10741 caagcgggag atatattggt agtaatggaa aatgaaaagg atgctaaaaa tattgcagaa
    10801 aaacgcaagg ataaaaagca cctcgaaagt atcaatgttt catcaaaggt gtcactcgat
    10861 gacttatatg aaagaattca aaagggtgaa gtaaaagatt taaatatcat tataaaagcg
    10921 gatgtaagcg gctcaataga agcagttaaa caatcattgc aaaaattgag cgtggacgaa
    10981 gtaaaagtta atcctataca cggtggtgtg ggtggaataa acgaaaatga tattttgctt
    11041 gcttctgctt caaatgcaat agtaatagga tttaacgtaa gaccttcggt tgcagctctt
    11101 gagcttgcaa aacaggaaaa tgtggatgta cgtacttaca atattattta caatgcaatt
    11161 gaagatattg agtctgctgt taagggaatg cttaaacctg tattcaagga agtagttatg
    11221 ggaagagctg aagtaagaca gacattcaag gtgccaaatg tggggattgt tgccggggtt
    11281 tatgtaacaa gcggaaagat tacaagaaaa tcgagaataa gattgctgag gaatgatata
    11341 gtaatacatg acggacaaat tgcatcgctg aaaagattta aagatgatgt gtctgaactt
    11401 agcagcggtt atgaaggcgg aataggtctt gacaaatata atgacattaa aataggtgat
    11461 tcaatggaag cctatataat ggaagaaata aaaagataaa aagaggtaag aaatgggtaa
    11521 tagaagaaac aacaggttat cgggcgaagt tagaaaagca atatccgaaa taatcataaa
    11581 tgatgtaaag gacccgagaa tttcagagct tatgagtata acggatgtac atgtaacaga
    11641 agatttgaaa tatgcaaaag tattcgtttc agactataac aatattgaaa ctactttaac
    11701 tgctttggaa agtgccaagg gttttataag aaaagaactg ggtaagaaag ttaaaatgag
    11761 aattattccc gagctgatat ttatcaaaga tgattcaatt gaaagaggct tgcacatgag
    11821 cagtcttatc gacaaagtta ttgaagaaga taaaacaaga aaggttaata aagataatgc
    11881 tgaagaatga tgtgaaggaa atatttaatt taataaataa ttctgacaaa atttgtatag
    11941 caggacataa ggcgcccgat ggagactgca tagggtcagt tatggccctt tataactttc
    12001 ttaaaccatt aaataaaaac atcacggtat tgatggacgg caatattcca tataattata
    12061 aagcctttat agatgaaaaa ataatttcaa acagctataa caatgaagaa tatgaccttt
    12121 tgatcgtatt ggacacaagt gacagaaaaa ggctgggcaa atttgaaaat gtgttaaaca
    12181 actcaaagaa aacagtagtt attgaccacc acaaaaccaa tgaaggcttc ggtaatataa
    12241 atataataga tacagaaatg agctccacgg gagaattggt atatgatata ttaaaagctg
    12301 cagatgctga tatttctctt gaagttgctg ctttaatcta ccttgccata ttaaccgaca
    12361 caggaaagtt ttcttataga aacactacca gcaatacaca caggaaagct gcagagctta
    12421 ttgatatcgg tgttaatgta tcggaaatag acaatattgt ttataattcc aaaccctcca
    12481 atatagtaag agcatttatg gagtgcgtaa gcggggttga gctttattat gacaacaaat
    12541 taggcataac ggctataaca aaaaatatac ttaataaaca caatgccgat atggctgata
    12601 ttgacggagt tgttgaatat atcagagaaa taaaagaagt tgaagtatcc tgcgtactta
    12661 aagaaaacga tgaaggtaca aaggtgagtc taaggtcaaa aaacaatata gatgttgctg
    12721 ctgtttcagt taaatacaaa ggcggcggtc atgcaaaggc cgcaggattt gatattaatg
    12781 aaaacataga aagtacaaaa aaaataataa ttgaggaatt taaaaagtat tttgcgtaat
    12841 gataggaata ttaaatatat taaaaccaac aggcatgaca tcccatgatg ttgtaagtaa
    12901 tgtcagaaaa atattaaata taaaaaaggt aggccatgca ggaactcttg accctaatgt
    12961 ggcaggagta ttggttattt gtgtcggtaa agcaacaaaa ttcagcgaat acttaatgag
    13021 cggcgacaaa gaatatgtat gtgagcttat attgggacaa agcaccgata ctcaggacag
    13081 ctacgggatg gttattgata aatctgataa agtatcagta acaagagaag agttaaatga
    13141 ggttttaaaa aaatttacgg gagagctaat tcaaattcct cctgcatatt cagcagttaa
    13201 actacatggt aaaaaactgt atgaatatgc aaggcaaaat atacaagtag agaaacaagg
    13261 aagaccgata aatattaaaa gtattgaact tcttaaattt gacgggaata aagctctttt
    13321 aaaagttaga tgcagcaagg gaacatatat tagaacttta tgcaatgata taggaaagag
    13381 cttgggtaca ttcggacaca tgggaatttt aatcagaaca gagtcaaaag gtttacatat
    13441 taataattct gtgacccttg aaaagttgaa atatttaaat gacagtaata agctggatga
    13501 gtgccttatt ccggttgaaa gtatttataa attagataaa ataaatgttg aatcgaaata
    13561 ttatgacagg ctgactgcag gaaatgaagt agaagcagga ttaaatacag acagcgataa
    13621 attctatgta tactgtcgaa acagtctgat aggtattgga aaaaaagtta aagataatac
    13681 aataaaaatt gacaaaatgc ttagataacg ggtgtgacat atgataaaaa caataggttc
    13741 atgtattgca ataggtaact ttgacggcat acatgtggga catgacgttc taatcagaag
    13801 aatgattgat ttaagtaaag aaagaaatct aaaaagtata ataataacat ttaaatatgt
    13861 aagaaatgac cttaaaaagt cttccatgaa tttgaaatac ataaacagtc cggaaacaaa
    13921 attggaaata ctgaaatctt acaatgtaga tgaagttatt gaaattgaat taaatgaaat
    13981 tatatcaaaa tattcaccag agcagtttat taagcttctg gtggatgatt ataatgcaaa
    14041 aaatattgta gtaggctata attttacttt cgggcacaaa gcaagcggca atataaatac
    14101 cttgaaagaa tttcaagata agtacggata tagagtggag gaaatacctc ccgtaaaata
    14161 taacggtatt gcagtaagtt catcattagt aagaacccta ttaaatgaag gtaaaattaa
    14221 tgaggctaat acacttttat taaataaata taccatctat aatgatgagt taaatattaa
    14281 ctataataaa aacataggtt ttgtagataa taaaagcagc attttagttc ctcccgacgg
    14341 gaaatatata gtaaaattag ggagtaaaga atatattcta aacattgttt ctaacaaaag
    14401 cggagctgct ttgatatttg ataaagaagt tgaagaaaac aaagatattg tgttcataaa
    14461 taaagcttct ta
//
LOCUS       DAOPUE010000044        14414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4287_length_14414_cov_10.486246, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000044 DAOPUE010000000
VERSION     DAOPUE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4287_length_14414_cov_10.486246"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..621
                     /locus_tag="PKH42_05470"
     CDS             85..621
                     /locus_tag="PKH42_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOA19914.1"
                     /translation="MNIGENLKRLRVANSLTQQELADRCELTKGYISQLERDLTSPSI
                     ATLTDILECLGTDLGSFFNEAVDSKIVFKQDDVFVKESEDNYIINWIVPNAQKYKMEP
                     ILVELGSGSKTKTDEPHEGEEFGYVLNGSILLNYGNQVYKVKKGESFYIKANKTHYIE
                     NVGKSKARVLWVSTPPYF"
     gene            634..1674
                     /locus_tag="PKH42_05475"
     CDS             634..1674
                     /locus_tag="PKH42_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA19915.1"
                     /translation="MNNAIIQLNNITKKFDGEIVLDDITLSVKENEFVTFLGPSGCGK
                     TTTLRIIGGFETPDEGEVYFDGKLINDVPPFKRQLNTVFQKYALFPHLNVFENIAFGL
                     RIKKMDEKTISEKVEKMLELFNLKNYGKRRIDQLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRKEMQHELKAMQKQLGITFIYVTHDQEEALTMSDTIIVMNEGIIQQMG
                     TPIDIYNEPENAFVADFIGESNILDGIMIDDYLVEFLGKRFECLDSGFGRGEEVDVVI
                     RPEDLDLTPAGEGKLEGIVKSVTFKGVHYEMDIVANNFEFLVHSTDMAPVGTTVGLTL
                     TPDDIHIMEKGE"
     gene            1677..2519
                     /locus_tag="PKH42_05480"
     CDS             1677..2519
                     /locus_tag="PKH42_05480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19916.1"
                     /translation="MKNRTKYFAYPYVLWIFLFIALPAFLVLLYSITTKESNGLTTIH
                     FTLENFKKFFQPMYLNILWDSIYLAAISTIICLILGYPAALIISRAHVSKRNTLLFLC
                     ILPMWMNMLLRTYAWMTILGNNGILNNIFAFLGLPRFNMLYTRGATVMGMVYNFIPFM
                     ILPIYTALSKMDKALLEAADDLGADKRTVFRKVIFPLSMPGVISGIIMVFMPAVSTFI
                     IPQLLGGNKSMMIGNLVEKLFILNGDWNFGSAISIVMMLIILLSMSIMNKFDVDKEGG
                     ARLW"
     gene            2513..3313
                     /locus_tag="PKH42_05485"
     CDS             2513..3313
                     /locus_tag="PKH42_05485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19917.1"
                     /translation="MVKKYLSKIYMGIIFIFLYAPIIVLGVFSFNESKSRGNWTGFSL
                     KWYIELFHDHDIRMAFYYTITIAIITAIVSTILGTIAAIGINAMKGRQQALILNVNYL
                     PVVNPDIVTGISLMILYISFNMDFGFMTMLLSHIVFSTPYVILSILPKLKQLDVHMTE
                     AAMDLGATPMYALRKVILPEIKPGIITGFLMAFTLSIDDFIISYFTKGEGVTNLSIVI
                     YSMARRGVRPTINALSTIMLIVVLLLMILINKRTNGHDKPKKIYRSEV"
     gene            3315..4355
                     /locus_tag="PKH42_05490"
     CDS             3315..4355
                     /locus_tag="PKH42_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOA19918.1"
                     /translation="MKRKLILTFLVLSLILIAATGCSSEEKQTLSILNYDIYIDKSLL
                     TEFEKANDVTIKYDTYSTPEEMYIKVKSGASKYDLIISSEYMIERMINEKLVNKLNFD
                     NIPNYKYIGEDFKNHVYDPDNEYSVPYFWGTLGILYNKKTVDASSDSWAMLWDENHAQ
                     KIIMMDSQRDSFAAALKLLGYSINTVDEGELDEAKELLIKQRPLVMAYITDGSPAIMV
                     TEEADMALVWSGEAVAAMSENENLDFVIPKEGSNIWIDAMVIPSTTQNQELAEKFINF
                     LCSTEATLRNIDEVWYSTVHTEAIKQVDEELLNNPAFNIPKEDIAKMEMLRDPKEFIE
                     LYTERWTELMAQ"
     gene            complement(4421..5257)
                     /gene="ytxC"
                     /locus_tag="PKH42_05495"
     CDS             complement(4421..5257)
                     /gene="ytxC"
                     /locus_tag="PKH42_05495"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtxC"
                     /protein_id="HOA19919.1"
                     /translation="MELLSLELNENENHEIYNNIRIITNIEESDINIELSSTDEGNIK
                     IRYFTDNKINKREYLEKITAKITKLLVEYTKLVGIKILKENYFYFDDEEVSTIISDIE
                     EEIDNDIKIHIIIKNKFKEVIEKSNYINLNGFVNFRLKFLKLYAAQVVEKCIDSYLMK
                     KEYMDFISIIKLISDSEENQYDIVNVMFNNNKIQVYDKNMQKLNYFSNVELSTELNTE
                     STYDETVINILMSVSPKKIILHDLKIDKKNKEAYNTADIIKRIFEGKVETCSGCKYCE
                     FL"
     gene            complement(5285..6019)
                     /locus_tag="PKH42_05500"
     CDS             complement(5285..6019)
                     /locus_tag="PKH42_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19920.1"
                     /translation="MNPYIPTQKPRYVIIDYRASEEILNYLKKLNIEPIKSIKCNELH
                     EPVAGHPDMVIFPIDFKTFVAAPNVYDYYRNVLGSLGIKVIKGGKTLSRNYPEDIAYN
                     VARIGRYALHNTKYTDQVLKYYLEEAGIQFIHVNQGYTKCSIAPVSDNKALTSDFLIH
                     EKLISYNIDCMYIDPKVVYLKGYNNGFIGGCTGLINEKIFLSTGKIFDENISVSLREF
                     IQSSGYIYDEASKQQITDLGTLIPII"
     gene            complement(6226..6420)
                     /gene="thiS"
                     /locus_tag="PKH42_05505"
     CDS             complement(6226..6420)
                     /gene="thiS"
                     /locus_tag="PKH42_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOA19921.1"
                     /translation="MKLNNRDFPWEEGLTIEKIIEMKSFTFPKIIVKVNGKHIEKEDY
                     PTTIINDGDNVEIIHLLAGG"
     gene            complement(6464..8620)
                     /locus_tag="PKH42_05510"
     CDS             complement(6464..8620)
                     /locus_tag="PKH42_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOA19922.1"
                     /translation="MDIIKMKENHKVLTEYKYELGEIDKGYTNRTLYVNISDNTITSK
                     PITEMMKEKFVGGRGFGLWYLWNATSPETKWDSPENEIIIAGGPVCGITQYAGTGKSL
                     VCSISPLTDIPIDSNVGGFFGPLLKFSGWDALEIQGKSDKDIIVYIDGNNGLVTIEEA
                     PLEAVDAHVLGEQLTEMYADNEDDKRNVAVVTAGTGAEHSRFGILNFTFYDPRRKVTR
                     LKQAGRGGIGTVFRNKKLKALIVKYSGVKGNLNNNFDQSIINKTGIKYHKEIHDLDHL
                     QNNMRKIGTANIIEIMDAYDLLPVMNFQYGSHPDTQNIKSTVFIEKYLTQGVQDGCWY
                     GCSMACAKGADNFLIKTGPYKGHRVTVDGPEYENAAGLGANCAIFDPEAILELNFYCD
                     TYGIDTISFGTATAFAMECYERGIINKEITGGLELNFGNFYASVELLHQMSKGEGFGT
                     VVGQGVHRMKKIFVEKYGADEEFLFDIGMEQKGLEYSEYGSKESLAQQGGYGLTNKGP
                     QHDEAWLIFMDMVNNQIPTFEDKAEALHYFPMFRTWFGLYGFCKLPWNDIVPADNKYT
                     DEPAKVPEHVQNYLDVVYGVTGKKLTVDDMIKESERVYNFQKIFNIRMGKGLRKNDKT
                     PYRSMGPVTKEEYESRQERYDKQLKEIIGYDIEGKTTEEKMAVLRKYRESQYEKLIDA
                     VYKRRGWNSNGVPTLEHLKYLDMELPELIEVIKDLQ"
     gene            complement(8630..9058)
                     /locus_tag="PKH42_05515"
     CDS             complement(8630..9058)
                     /locus_tag="PKH42_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA19923.1"
                     /translation="MKYLAKDDSKCIQCGACESICSKTYFKEDNPEKSCIQIKKEDTP
                     EINVCNQCGECIDICPVMAIKRDKFGVVRIDKKICVGCFMCVGFCPLLSMRQHDDYIE
                     PFKCIACGLCAKECPTEAIYIAERSDEEAREMPIDKVFNR"
     gene            complement(9207..10088)
                     /gene="hslO"
                     /locus_tag="PKH42_05520"
     CDS             complement(9207..10088)
                     /gene="hslO"
                     /locus_tag="PKH42_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOA19924.1"
                     /translation="MDYMIRAMDKKHTFRLFMVRATNTVEEARQHHNTTPTASAALGR
                     TLIAGLMMGYMMKNEKDRLTININGGGPIGTILVVSDNSGHVKGYVGNPNVDLDLKEN
                     GKLNVGGAVGTQGKLTVMMDIGLKEPYVGSTDLVTGEIGEDIAFYYYLSEQQNTAVAL
                     GVLVDIDYSIKAAGGFMVQTLPFIEEEDLVKLENTLNNLKSISDYFNNEKDLEEIAKE
                     IFSDFDIEVFDRIPVSFNCDCSEERMEQALISLGKNELKKIIEEDGKIETVCHFCNKK
                     YMFEGDKLKNILKYIEE"
     gene            complement(10091..10825)
                     /locus_tag="PKH42_05525"
     CDS             complement(10091..10825)
                     /locus_tag="PKH42_05525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA19925.1"
                     /translation="MKNSYKEFSQIYDLLMDIDYEKWTSFIMKKLKGKRKILEAACGT
                     GGITKLLADNNYKVTAFDLSEDMLMRAYEKLAQSPMVRLLNQNMVDFEIDEKFEAAIC
                     CCDGINYLIKEEVKSFFQRIYHHLNEGSKFIFDMSTKYKYDKMFCDTYVYDDGDIFYV
                     WENELVNDEKINIEINFFIKDTDKYTRITEEQTQYIHTVDEIIDLLEDAGFENIEIFD
                     DYNEKEYSDTSLRAVFSACKFDNERT"
     gene            complement(10837..11037)
                     /locus_tag="PKH42_05530"
     CDS             complement(10837..11037)
                     /locus_tag="PKH42_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOA19926.1"
                     /translation="MAEKKEKNKKNKKKKEPTPNDIMKMEIAEELGLTDKVKKFGWGG
                     LTAKETGRIGGLMTAKKKKRKK"
     gene            complement(11094..11231)
                     /locus_tag="PKH42_05535"
     CDS             complement(11094..11231)
                     /locus_tag="PKH42_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19927.1"
                     /translation="MLDFDFRVIAVVMFFIILISIQFTLNKIFLLLREIRAILLTKRD
                     R"
     gene            complement(11339..11791)
                     /locus_tag="PKH42_05540"
     CDS             complement(11339..11791)
                     /locus_tag="PKH42_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA19928.1"
                     /translation="MYDIKKILVPVDGSPASIKASERAIEIAKKYGSEITFLSVANTP
                     DMFNIGDYSFRVHYNYDNVVNNHKLAQAEILDKVIEGLDIEGVKFEKKVISGEPAEEI
                     IRMAHDCQYDYIVMGRRGFSKFTRFFVGSVTQRVIAESPCAAVIVVNE"
     gene            12005..12718
                     /locus_tag="PKH42_05545"
     CDS             12005..12718
                     /locus_tag="PKH42_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOA19929.1"
                     /translation="METKKETIIKAAGAAFPQTIPILAGYVFLSIAYGIYMNAFGFNF
                     LYPMFMSMLIYAGTVQFVAVGLLMAVFDPINAFVLTLMINARYLFYGISMLEKYRVKG
                     IRGAFLIFSMSDETFSINCTANIPEDVDKGWFMFFVTLFNYCYWVSGSILGGLFGELI
                     EFNTKGLEFAMTAMFIVIFMEQWLKEKSHSSSLLGLGISLLCLIIFGSKNFIIPSMIL
                     ILLILSLKRELFMEAGESK"
     gene            12715..13047
                     /locus_tag="PKH42_05550"
     CDS             12715..13047
                     /locus_tag="PKH42_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HOA19930.1"
                     /translation="MTMTVGQHIITIAMVVLGTMITRFLPFIIFPADKPTPKYIQYLG
                     KALPPASLGLLVVYSLKDVNIFTGSRGIPELIAVITVLVIHLWKRQMLLSIAVGTITY
                     MLLVQFVL"
     gene            complement(13106..13187)
                     /locus_tag="PKH42_05555"
     tRNA            complement(13106..13187)
                     /locus_tag="PKH42_05555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13151..13153),aa:Leu,seq:cag)
     gene            complement(13272..14015)
                     /locus_tag="PKH42_05560"
     CDS             complement(13272..14015)
                     /locus_tag="PKH42_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA19931.1"
                     /translation="MENKVLAIVDGREIKESDLKNLLRNLGQNAVHFQGEEGRKKLID
                     ELVMHELMYSDAVENNLENEEEFIEVFNDMKKSMLQQYNLRKMFNKVTISDEELKDFY
                     EKNKYFYNRPEMVKASHILVDTEERANEILEDITDGLSFEDAAKEYSNCPSKQDGGFL
                     GQFGKGQMVKEFEDAVFSMRAGEISPPVKTQFGYHIIKLIEHMPARSAEFEEVYQEVK
                     DNYFVIKQEEVYKSKKEELIKKYDVVLFE"
     gene            complement(14065..14331)
                     /locus_tag="PKH42_05565"
     CDS             complement(14065..14331)
                     /locus_tag="PKH42_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOA19932.1"
                     /translation="MRSQKVVLKNKIGLHARPAALFVKKARNFDSEIIIKKGIQEANG
                     KSIISIMALGAMKGDELVIITKGSDEDKCIAELTNLLEFMEEEE"
BASE COUNT         4274 a         2680 c         2288 g         5172 t
ORIGIN      
        1 aaggggtgtg gatatgtata ataaaagctg tttcagtgtt tagtaatttt aaactttgag
       61 tttagtaaaa aatgtgaggt ttatatgaat attggagaaa atttaaaaag gttgagagtt
      121 gccaattctc tcacacagca ggaattggct gacagatgcg aattgacaaa ggggtatatt
      181 tcgcagcttg agagagattt gacttctcct tccatagcaa ctcttacgga tatattggag
      241 tgtctgggga cggatttagg aagctttttc aatgaagccg tggacagtaa aattgtgttt
      301 aaacaagatg atgtatttgt aaaagaatca gaggacaatt atattattaa ctggattgtt
      361 ccaaatgcac agaaatataa aatggagccc atattggtgg aactcggaag cggcagcaaa
      421 acaaaaacag atgagcctca tgaaggcgaa gagttcggtt atgttttaaa cggctccata
      481 cttttaaact acggcaatca agtgtacaag gttaaaaagg gagagtcttt ttacattaag
      541 gcaaacaaaa ctcattatat tgaaaatgtg ggaaaaagca aagcgcgtgt actatgggta
      601 agtactccgc cgtattttta gcaggaggta agaatgaata atgcaattat acaattaaat
      661 aatattacca aaaaatttga cggagaaatt gttcttgacg atattacctt atccgtgaag
      721 gaaaatgaat tcgtaacttt tttgggacca agcggctgtg gaaaaacaac tactcttaga
      781 attatcggag gctttgaaac tcctgatgaa ggtgaagttt attttgacgg caaattaatc
      841 aatgacgttc ctccttttaa aagacagctg aataccgtgt ttcagaaata tgctttgttt
      901 cctcatttaa atgtttttga aaacatagca tttggtctga gaataaagaa aatggatgaa
      961 aagaccattt ccgaaaaggt tgaaaaaatg cttgagcttt ttaatctcaa aaattacggc
     1021 aaaagaagga ttgaccagtt aagcggagga cagcagcagc gtattgcaat agcaagagct
     1081 ttggtaaatg agcccaaggt acttttgctc gatgaaccct taggtgcttt ggatttaaaa
     1141 ttaagaaaag aaatgcagca tgagctcaaa gcaatgcaga aacagcttgg aataacattt
     1201 atttatgtaa ctcatgacca ggaagaagct ttaacaatgt cagacacgat tatagtcatg
     1261 aatgaaggca ttattcaaca aatgggtact cccatagata tatataatga gcctgaaaat
     1321 gcgtttgtgg cagattttat cggagaaagc aatatattgg acggaattat gatagatgat
     1381 tacttggttg aatttttagg aaagagattc gaatgtctcg attcaggatt tggaagaggc
     1441 gaagaagttg atgtggttat tcgccctgaa gatttagatt taactcctgc cggtgaagga
     1501 aagctggagg gaattgtaaa gtcggtaaca tttaaaggtg ttcattatga aatggacata
     1561 gtagcaaata attttgagtt tttggttcat tcaaccgata tggcccctgt aggaacaact
     1621 gtcggtttaa ccttaactcc tgacgatatt catataatgg aaaaggggga atagctttga
     1681 aaaacagaac aaaatatttt gcttacccct atgtcttgtg gatatttttg ttcattgctt
     1741 taccggcatt tttggtgctt ttatacagca ttaccacaaa agaatctaac ggacttacaa
     1801 cgattcattt tacattagaa aatttcaaga aattttttca acccatgtat ttgaacattt
     1861 tatgggactc catctactta gcggcaattt caaccataat atgtttgata ctcggatacc
     1921 ctgcggcatt aataatttct agggcacatg tgtcaaagag aaatactttg ttgtttcttt
     1981 gtattttgcc catgtggatg aatatgctcc ttagaactta tgcttggatg accatacttg
     2041 gaaacaatgg aattttaaat aacatttttg cttttttagg tcttccgagg tttaacatgc
     2101 tttatacaag gggcgctacc gtaatgggta tggtttacaa ctttattccc tttatgatac
     2161 tgcccattta tactgcatta agtaaaatgg ataaagccct tttggaagca gccgacgatt
     2221 tgggagcaga caaaagaaca gtttttagaa aagttatttt ccccttaagc atgcccggtg
     2281 taatttcggg catcataatg gtttttatgc ctgcggtaag cacatttatc attcctcaac
     2341 tcttaggagg aaataaaagc atgatgatag gaaacttggt ggaaaaattg tttattttaa
     2401 acggagactg gaatttcggc tcagccatat ccattgtaat gatgcttata attcttttat
     2461 ccatgtcaat tatgaataaa tttgatgttg ataaggaagg aggagcaagg ttatggtaaa
     2521 aaaatattta tcaaaaatat atatgggcat tatatttata ttcctctatg ctccgattat
     2581 agttcttggt gtattttcct ttaatgaatc aaaatcgaga ggaaactgga caggattttc
     2641 actgaagtgg tacattgagc ttttccatga ccacgatata cgaatggctt tttattacac
     2701 aataacgata gcaatcatta cggcaatagt atctacaatt ttgggtacaa tagcagcaat
     2761 aggaataaat gcaatgaagg gcagacaaca agctttaata ctcaatgtta attaccttcc
     2821 agttgtaaat cccgatattg ttacgggtat atccctgatg attttatata tttcatttaa
     2881 tatggatttt ggatttatga caatgctatt gtctcatata gtgtttagca ctccctatgt
     2941 aatattgtca atacttccaa agttaaagca gctcgatgta cacatgaccg aagctgcaat
     3001 ggatttaggg gcaactccca tgtatgctct aagaaaggta atacttcctg aaataaagcc
     3061 gggaattatt acgggatttt tgatggcatt tacattgtcc attgatgatt tcatcataag
     3121 ttactttacc aagggcgaag gtgttacaaa cctttcaatt gtgatttatt caatggcaag
     3181 aagaggtgtc cgccctacaa tcaacgctct ttcaacaata atgctgatag tagtattatt
     3241 gttaatgata ttgataaaca aaagaactaa cgggcatgat aaacccaaaa aaatatatcg
     3301 aagtgaggta tagaatgaaa agaaaattaa ttttgacatt tttagtttta agcttaatat
     3361 taatagctgc aaccggatgc agctctgaag aaaaacaaac attgagtatt ttaaactatg
     3421 atatttacat tgacaaaagt ttattgactg aatttgaaaa agccaacgat gttacaatta
     3481 aatatgacac gtattcaaca ccggaagaaa tgtatataaa ggtgaaatcc ggggcttcaa
     3541 aatatgattt gattatttca agcgaatata tgattgagcg catgattaat gaaaaattgg
     3601 ttaataaatt aaatttcgat aacattccga attataaata tatcggcgaa gattttaaaa
     3661 accatgtata tgaccctgat aacgaatatt cggttccgta tttctggggg accttaggca
     3721 tattgtacaa taaaaaaact gtagatgcat cttctgacag ctgggctatg ctttgggatg
     3781 aaaatcatgc tcaaaaaata attatgatgg attcgcaaag agattcattt gcagctgcgc
     3841 taaaattatt gggatattca attaacacgg ttgatgaagg agagctcgat gaagcaaagg
     3901 aactccttat taagcaaaga cctcttgtta tggcttatat aacggacggt tcacctgcaa
     3961 ttatggtaac cgaagaagct gatatggcgc ttgtttggtc aggtgaagcg gttgccgcaa
     4021 tgtccgaaaa tgagaatttg gattttgtaa ttccaaagga aggcagcaat atttggattg
     4081 atgctatggt tattccttcg actacccaaa atcaggaatt agccgaaaaa tttattaact
     4141 tcttatgctc caccgaagca acgttaagaa acatagacga agtatggtat tctacagtac
     4201 ataccgaggc tattaaacaa gtggatgaag aactcttaaa caaccctgct tttaacattc
     4261 caaaggaaga tatcgcaaag atggaaatgt tgagagaccc gaaagaattt atagaattat
     4321 acactgaaag atggactgaa ttaatggcac agtaatcatc tttaaattaa aaccggtgcc
     4381 gggcacccaa tagggtgccc ggcaccgctt catatatatt ttataaaaac tcacaatact
     4441 tacatccgct gcaagtttcg actttacctt caaatattct ttttattata tcagcggtat
     4501 tatatgcttc tttatttttc ttatctatct ttaaatcatg caatattatt ttttttggtg
     4561 acacactcat taaaatattt attacagttt cgtcgtatgt tgattcggta ttcaattcag
     4621 ttgaaagctc tacatttgaa aaataattga gcttttgcat attcttgtca tatacctgga
     4681 ttttattgtt attaaacatt acatttacaa tatcatattg attttcttcc gaatctgata
     4741 tcagttttat aatacttata aaatccatgt attccttttt cattaaatat gagtcaatgc
     4801 acttctctac tacctgtgct gcataaagct ttagaaattt tagtctgaag tttacaaatc
     4861 cgtttaaatt tatataattg gacttttcta taacttcttt aaatttattt ttaattatta
     4921 tgtgtatttt aatatcattg tctatttcct cttcgatatc ggatattatt gtgcttactt
     4981 cttcatcgtc aaagtaaaaa taattttcct ttaaaatttt tataccaact aacttagtgt
     5041 attcaaccaa aagcttggtt atttttgctg ttattttttc aaggtactct ctcttgttta
     5101 ttttattatc cgtaaaatat cttattttta tatttccttc atcggtagac gataattcta
     5161 tatttatatc actttcctct atgttagtta taatccttat attattataa atttcatgat
     5221 tttcattttc atttaactct aatgatagta gctccactta gtcactcctc tcggtcaagg
     5281 gaggttatat tatcggtatc agcgttccta agtctgtaat ttgctgtttt gaagcttcat
     5341 cataaatata tcctgacgac tggatgaatt ctcttaatga aacagaaata ttttcatcaa
     5401 aaatttttcc ggttgacaaa aatatttttt cattaatcaa tcctgtacag cctcctataa
     5461 atccgttgtt gtatcctttt aaatatacaa cttttgggtc tatatacatg cagtctatat
     5521 tgtatgatat tagtttttca tgtatcaaga aatccgatgt caaagcttta ttatcactga
     5581 caggagcaat tgagcacttt gtatagcctt gatttacatg aatgaattga atgcccgctt
     5641 cttccaaata atattttaaa acctggtctg tatactttgt attatgcaaa gcatatctcc
     5701 ctattcttgc tacattatat gctatatcct caggatagtt tctgctaaga gtttttccac
     5761 ctttaataac ttttattcct aggcttccca aaacatttcg gtaataatca taaacgtttg
     5821 gagctgcaac aaaagtttta aaatctatgg gaaaaattac catatccgga tgaccggcca
     5881 caggctcatg cagctcattg catttaatgg attttatagg ctctatattt aattttttta
     5941 gataattcag tatttcttca cttgctctgt aatcaataat tacatagcga ggcttttgag
     6001 tcggaatgta tggattcata ttatctcccg ttgaatgagt ctcattcaca gattatatca
     6061 ttaaggtata tattattcta caaaaaatca tgaggttctt caagctagct ctcagaacga
     6121 caaaaatgct atttatagta aaataaaatg atataattat aaaattatat catttttata
     6181 ttaatccaaa taaatttatc aggcaatttc tacaagtgag ttgatttatc ctcctgccaa
     6241 gagatgtata atttcaacat tatcaccgtc attaataata gttgttgggt aatcttcctt
     6301 ttcgatatgt tttccgttta ctttaactat tattttagga aaggtgaaac ttttcatctc
     6361 tataattttt tctattgtca atccctcttc ccagggaaag tctctattgt ttagttttat
     6421 aatttatacc acctccgaat gtaactatac tcccgtcccc ttgttattgc aggtctttga
     6481 ttacttctat taattcagga agttccatat ccaaatattt tagatgttct aatgtcggga
     6541 cgccgtttga attccatcct cttcttttat agactgcatc tataagcttt tcatattggc
     6601 tttctctata ctttctcagt accgccatct tttcttcggt agtcttgcct tctatatcat
     6661 acccgattat ttctttaagc tgtttgtcgt atctttcctg ccttgattca tattcttcct
     6721 tggttaccgg acccattgag cggtaaggag ttttatcgtt tttccttaaa cccttaccca
     6781 ttcttatgtt aaaaattttc tggaagttat aaaccctttc agattctttt atcatgtcat
     6841 caacggttaa tttcttgcct gtaactccat ataccacatc aagataattt tgaacatgtt
     6901 caggcacttt tgcaggctca tcggtatatt tattgtccgc cggaactatg tcattccaag
     6961 ggagcttgca gaatccatac aagccgaacc aagtcctgaa cataggaaag taatgaagag
     7021 cttcagcctt atcttcgaag gttggtattt gattgtttac catatccatg aaaatcagcc
     7081 atgcttcatc atgctgagga cctttgtttg ttaaaccgta tcctccctgc tgagccagag
     7141 attctttcga gccgtattcc gaatattcaa gccccttttg ctccattcct atgtcaaaca
     7201 agaattcttc atcagctccg tatttttcaa cgaatatttt tttcatcctg tggactccct
     7261 gacctacaac tgttccaaag ccttctccct tggacatctg gtgcaagagt tcgactgatg
     7321 cataaaagtt gccgaaattc aattctaagc cgcctgtaat ttccttgttg attatacctc
     7381 tttcatagca ttccattgca aatgccgttg cagtaccaaa tgaaattgta tctatgccat
     7441 aggtgtcgca atagaagttt aattccaaaa ttgcttccgg gtcaaaaata gcacaatttg
     7501 ctcctaatcc tgccgcattt tcatattcgg gaccgtctac tgtaactcta tgtcccttat
     7561 aaggtcctgt ctttatgagg aaattatccg cacctttggc gcaggccata gagcagccgt
     7621 accagcagcc gtcttgaact ccctgagtca ggtatttttc aataaatacc gtggatttaa
     7681 tattttgtgt gtccggatgg cttccatatt ggaaattcat aacaggaagc aaatcatagg
     7741 catccattat ttcaataata tttgccgtgc ctatttttcg catattgttt tgcaaatggt
     7801 ctaagtcatg aatttcctta tgatatttta ttccggtttt attaattatt gactggtcaa
     7861 aattattgtt taaattgcct tttacaccgc tgtattttac aatcagtgcc ttaagctttt
     7921 tatttcgaaa tacggttcct attccacccc tgcctgcttg ttttagtctt gttacttttc
     7981 ttctggggtc atagaatgta aaatttaata ttccaaaacg ggaatgctcg gcacctgttc
     8041 ctgcggttac aacagctaca tttcttttat catcttcatt gtccgcatac atttcggtaa
     8101 gctgctctcc caaaacatgt gcatcaacag cttccaatgg tgcttcttca attgttacaa
     8161 ggccattatt cccatcaata tatacaatta tatctttatc tgatttgccc tgtatttcaa
     8221 gagcgtccca tcccgaaaat ttcaaaagag gtccaaaaaa tccgcctaca tttgaatcta
     8281 tgggaatatc cgttaagggt gaaatgctgc ataccaatga ttttcctgtt cctgcatatt
     8341 gagttatacc gcagacagga ccccctgcaa taattatttc attttccggg ctatcccact
     8401 tcgtttcagg acttgttgca ttccacagat accacaagcc aaaacccctt ccccctacaa
     8461 atttttcttt catcatttcg gttatcggct tagaggttat tgtattgtct gaaatattaa
     8521 catacagtgt tctgtttgta tatcctttgt ctatttctcc gagttcatat ttatattcgg
     8581 ttaagacttt atgattttct ttcattttta taatatccat tttgtcctcc tatctgttaa
     8641 aaaccttatc tattggcatt tctcttgctt cctcatcgct tctttcagct atataaattg
     8701 cctccgtagg acattctttg gcacataatc cgcatgctat acatttaaaa ggctctatat
     8761 aatcatcatg ctgcctcatg gataagagag ggcaaaatcc cacacacata aaacaaccga
     8821 cgcaaatttt cttgtcaatt ctaacaaccc caaacttgtc cctctttatt gccataacag
     8881 gacaaatatc gatgcattct ccgcattgat tgcaaacatt tatttcaggg gtgtcctctt
     8941 ttttgatttg tatgcatgac ttttccggat tatcctcttt aaaatatgtt ttagagcata
     9001 tgctttcgca agccccgcac tggatacatt tactgtcgtc tttagccaaa tatttcatac
     9061 tgacctcctc ttgtatgtta atattaatta gatttcattc atttataatc acttatatta
     9121 tataaatatt tttaatatat ttcaatttct tatagtatta ttaatgtgct aatatttttc
     9181 tattagttgc tccttttctc gcaatatcat tcttcaatat atttcaaaat attttttagt
     9241 ttgtctcctt caaacatata tttcttattg cagaaatggc atactgtttc tattttcccg
     9301 tcttcctcaa ttattttttt cagctcattc ttacccaatg aaattaatgc ctgctccatt
     9361 ctttcctcgg agcagtcaca attaaaagaa acgggaattc tgtcgaaaac ctcgatatca
     9421 aaatcggaaa atatttcttt tgcaatttct tctagatctt tttcgttatt aaaataatct
     9481 gatattgatt ttagattatt gagggtattt tctaatttaa caaggtcttc ttcctcaata
     9541 aaaggcagtg tttgaaccat aaagcctcct gcggctttaa tgctgtaatc tatatccact
     9601 aatacaccca aggctacggc agtattttgc tgttccgaca aataataata aaatgctatg
     9661 tcttctccaa tttctcctgt taccaaatcg gtgcttccca catagggctc cttcaatcct
     9721 atatccatca taacggtgag cttgccttga gtcccgacag ctcctcctac gtttaattta
     9781 ccgttttctt tcaagtctaa atcaacattc ggattgccaa catacccctt aacatggcct
     9841 gaattatccg aaacaaccaa aattgtacct atgggacctc caccgtttat attaattgtt
     9901 aatctgtctt tttcgttttt catcatatag cccatcatga gaccggctat aagagtcctg
     9961 cctaaagcag cagaagctgt cggtgtcgta ttatggtgct gccttgcttc ttccaccgta
    10021 tttgttgccc ttaccataaa aagtctaaaa gtatgttttt tatccattgc tctaatcatg
    10081 taatccattg ttatgtcctt tcattgtcaa atttgcatgc ggaaaaaact gcccgcaaag
    10141 aggtgtcaga atattctttt tcattgtagt catcaaatat ctcgatattt tcaaatcctg
    10201 catcctcaag taagtctatt atctcatcaa ctgtatgaat atattgagtt tgttcttcag
    10261 taattcttgt atatttatct gtgtccttaa taaaaaagtt tatttcaata tttatttttt
    10321 catcattaac aagttcattt tcccaaacat aaaaaatatc tccgtcatca taaacataag
    10381 tatcacaaaa cattttatca tatttgtatt ttgtgctcat gtcaaaaatg aatttggagc
    10441 cttcatttaa atgatggtaa atcctttgga aaaaagattt cacttcttcc tttatgaggt
    10501 aatttatgcc gtcgcagcag caaattgctg cttcaaattt ttcatctatt tcaaaatcaa
    10561 ccatattttg attcaataat cgaaccatag ggctttgcgc cagcttctca taagccctca
    10621 taagcatgtc ctcagacaaa tcaaaggcag ttaccttgta attgttatcg gctaaaagct
    10681 ttgttatgcc tcccgtgccg caggcagctt ccaagatttt tctttttccc tttaatttct
    10741 tcattataaa ggatgtccac ttttcgtaat cgatatccat aagcaaatca taaatttgag
    10801 aaaattcttt atagctgttt ttcatttact tttcctttac tttttccttt tctttttctt
    10861 agctgtcatt aatcctccga ttctgcctgt ttccttggca gttaatcctc cccagccgaa
    10921 ctttttaact ttatccgtaa gtccaagctc ttctgctatt tccatcttca ttatatcgtt
    10981 tggtgtcggt tcttttttct tcttgttttt cttgttcttc tctttctttt cagccataat
    11041 accctccttg taatattatg gcttttaaaa ggaaacttat tctaataatc tgtttatcta
    11101 tctctcttag tgagtaaaat tgcccttatt tctcttaaaa gcaaaaatat tttattcaga
    11161 gtaaattgta tacttattaa aattataaaa aacattacaa cggctattac tctaaaatca
    11221 aaatctaaca tattttctcc ttgaaatgag accatctttt ttcaataata tcacaagaaa
    11281 aaaaccattt caagaaaaaa ccccctgggt cagggggaaa tatatatagt tttgtatatt
    11341 attcattaac aacaataacc gcagcacatg gggattcagc gataaccctt tgagttaccg
    11401 agcccacaaa aaatcttgtg aattttgaaa atcctcttct tcccattaca atataatcat
    11461 attgacaatc gtgtgccatt cttattattt cttcagcagg ttctccggaa ataacctttt
    11521 tttcaaattt aaccccttct atatccagac cttctataac tttatccagt atttcagctt
    11581 gagctaattt gtgattattt accacattat catagttata gtgtactctg aaactataat
    11641 caccaatgtt aaacatatcg ggtgtatttg ccaccgataa aaatgtaatc tcactcccat
    11701 acttctttgc aatttctata gctctttcag atgcctttat cgaagccggc gagccgtcaa
    11761 ccggtaccaa tattttcttt atatcataca taattaattt atctcccttc attaatattg
    11821 tttgatatat atatacctgt ttaaattcca attaaacatt ttttaacaaa atttattgtt
    11881 tataattctt ttgattgatt atgtgcagtt tcagtgatag aatatagcga agtaaaaatt
    11941 tctcctcttt gggtaagggg tatggattaa attacataaa tttgattagg agtcatagaa
    12001 atagatggaa acaaaaaaag aaaccataat aaaagctgcc ggagcagctt ttcctcaaac
    12061 cattcctatt ttggcaggct atgtattctt aagcatagcc tatggtattt atatgaatgc
    12121 tttcggattc aattttttat atcccatgtt tatgagcatg ttaatctatg caggtacagt
    12181 tcaatttgtt gcagtaggtc ttctgatggc agtcttcgac cctataaatg cttttgttct
    12241 gacacttatg attaatgcac gctatttgtt ttacggcatt tctatgttgg aaaaatatcg
    12301 ggttaaggga ataagaggag cctttttgat ttttagtatg agtgatgaaa cattttcaat
    12361 caattgcacg gcaaatatcc cggaagatgt cgataagggt tggttcatgt tctttgtaac
    12421 tctttttaat tactgctatt gggtatcagg ctccatactt ggcggattgt tcggtgaatt
    12481 gattgaattt aatacaaagg ggcttgaatt tgcaatgaca gcaatgttca ttgtaatctt
    12541 tatggagcag tggctgaaag aaaaaagtca tagcagctcg ctgttaggat tggggatatc
    12601 tttgttatgc ctaattatct ttggcagcaa aaactttata ataccttcaa tgattttaat
    12661 tttattaatc ctttctctaa aaagagaact ttttatggaa gcaggtgaat caaaatgacg
    12721 atgactgtcg gacaacatat tataacaatt gccatggtag tacttggtac aatgattacc
    12781 cgttttcttc cctttataat ttttcctgcc gataagccaa caccaaaata tatacagtat
    12841 ttgggaaagg cgctcccgcc ggcttccctg ggactgcttg tagtgtacag tcttaaggat
    12901 gtaaatatat ttacgggcag tcgcggtatt ccggagctga ttgccgttat tacagtactt
    12961 gttatccacc tgtggaaaag acagatgctt ttatccattg ctgttggaac aataacttat
    13021 atgctcttgg ttcaatttgt actatgactt tctaccatta aaaaacggct ccctgtcgga
    13081 agccgttaaa aagttgaatt attagtgcgg ataacaggat ttgaacctgc acaggttgcc
    13141 ccacacggac ctgaaccgtg cgcgtctgcc aattccgcca tatccgctgg tcactgaaat
    13201 atgttatcac aaatatataa tctttgcaat atttttttcg tgctgtctca actttctcaa
    13261 taagttatca attattcaaa taatacaaca tcatactttt taattaattc ttctttttta
    13321 cttttataaa cttcctcttg tttaattaca aaataattgt cttttacttc ctggtaaact
    13381 tcttcaaatt ctgcacttct agcaggcata tgctcaatta atttaattat gtgatatcca
    13441 aactgtgttt ttacaggtgg acttatttca cctgcgcgca ttgaaaatac agcatcttca
    13501 aattctttta ccatttgccc ttttccgaac tgtcccaaaa atcctccgtc ttgcttggaa
    13561 ggacagtttg aatactcttt agcggcatcc tcaaaagaca aaccatctgt tatatcttcc
    13621 aatatttcat ttgctctttc ctctgtatca accaagatgt ggcttgcttt taccatttcg
    13681 ggtctattgt aaaaatactt gtttttttca taaaagtctt tcaattcttc gtcggatatt
    13741 gtaactttgt tgaacatttt tcttaagttg tattgttgca gcatcgactt tttcatatcg
    13801 ttgaatactt cgataaattc ttcttcattt tccagattgt tttctaccgc atccgaatac
    13861 ataagttcat gcataaccaa ttcatcaata agcttttttc ttccttcttc tccttgaaaa
    13921 tgcactgcat tttgtcctaa attccgtaat aaatttttta agtctgattc ttttatttct
    13981 cttccgtcta ctatagctaa tactttattc tccatttttt ctccttcgtc ataaaataag
    14041 agggtctttt ccgcgccctc tttattattc ttcttcttcc ataaattcca ataaattagt
    14101 caactcagca atacatttgt cctcgtctga acctttggtg attattacta attcatcgcc
    14161 cttcatggct cccaaagcca ttatgctgat aatactcttg ccgtttgctt cctgtatacc
    14221 ttttttgatt ataatttcac tgtcaaaatt tcttgccttt ttaacaaata aagccgcagg
    14281 tctggcatgc agtccaatct tattttttaa cactactttt tgtgatctca ctttattaca
    14341 cctctctttg tatgctcgat taaatcttca gcaatttctt ctatttttct tagtctgtga
    14401 tttactcctg attt
//
LOCUS       DAOPUE010000045        14395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4294_length_14395_cov_10.848117, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000045 DAOPUE010000000
VERSION     DAOPUE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_14395_cov_10.848117"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1263)
                     /locus_tag="PKH42_05570"
     CDS             complement(1..1263)
                     /locus_tag="PKH42_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOA19933.1"
                     /translation="MGSILLLTFLGCLALGVPIAFSVAVAGVVVLIVGDVNLLIVVQR
                     LFASSDSFSLVAIPFFILAGDLLARGEMSKMLVAFAEACIGKVKGGLSAVSVLASMFF
                     AAISGSGAATTAAVGTSMLPELEKRGYEVDRAAALIASGGCIGVVIPPSVPMVLYAVI
                     AEQSVTRLFKNGFFPGFMMGGILIAISLYEAKKFNYPDGRAFEFKYMVETFRKAVWAL
                     IMPLIILGGIFSGYFTPSEAAGVAVVYSMFASMFIYKDLTLKELGQIMLGSGKTSAII
                     MTIIACSGVFGWALANWQIPEAVAQAVLSVSDNKYIILLLMAVIVLIAGVFMETSSAI
                     ILITPVFLPLVRMLNIDLIHFGLIFTIGIAIGMITPPVAIDLFVASSITGMPIERIAK
                     KVVPYLIGLIIVFFIYIYLPLFIPSLIL"
     gene            complement(1263..1757)
                     /locus_tag="PKH42_05575"
     CDS             complement(1263..1757)
                     /locus_tag="PKH42_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOA19934.1"
                     /translation="MVVIAKVIKKASDFIDKIGCSLLILTMAGMVFFSSIQIIARVFF
                     TALAWSEELTRYLLVWSTFIGAGCVYKRGGHINVSFVQDKFKGKANKYAKILVHLICI
                     AFFIIAVYYGVIYMMKQGAQSSPALGIRMNLMYMAIPLGCGVMLLHALNAIFEILVTR
                     EVAE"
     gene            complement(1781..2782)
                     /locus_tag="PKH42_05580"
     CDS             complement(1781..2782)
                     /locus_tag="PKH42_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOA19935.1"
                     /translation="MKKLNFIALALIVVMVLGTACGQTVDNPPTSAEPTVIKLGMTIN
                     EQDSFYVCATKFSELVEERTNGAYDIQLFPNSSLGDERTMLESMQMGTLDSGIITSGP
                     FVNFVPEFGVLDLPFLFPDNASAYKVLDGEIGKEILSKLEDHDLKGLAYAERGFRNLT
                     NSVRPVRTAEDIKDLKIRVMENEVYLSAFKAMDVNAVPMAWTEALTALQQGTIQGQEN
                     PVNVIHAYKMWDSQKHVTMTRHAYAAAVITFSEEKFNSLPADVQEIFVQAAQEAAEYE
                     RAWVAENEEAQIADLKANGMELIEEPDLESFRQAVQAVYEQYPQYSEYLTRIKEAIK"
     gene            complement(2823..4484)
                     /gene="ilvD"
                     /locus_tag="PKH42_05585"
     CDS             complement(2823..4484)
                     /gene="ilvD"
                     /locus_tag="PKH42_05585"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOA19936.1"
                     /translation="MRSDQMKSGVERIAHRALFNALGLTEKEIRQPMVGIVNAQNDII
                     PGHLHLDDIVEAVKSGVMMAGGTPFVFPAIGVCDGIAMGHEGMKYSLISREHIADSVE
                     IMAMAHPFDALVFVPNCDKIVPGMIMAALRLNIPSIFVSGGPMMPGYYDGKKLTVSNS
                     FEAVGAAGAKKMSDERIKEIELYSCPGCGSCAGMFTANSMNCMTEVIGLGLSGNGTIP
                     AVHAARIRLAKEAGMKVMELLEKDIRPRDIVTLDSLHNALTADMALGCSTNTVLHLPA
                     IAHEAGLKIDLNEINNISKATPHLVKLSPAGEDCLVDLWQAGGIPSVLKELDNHGLLN
                     TDCITVTANTVGENIKDAYIKDNSVIRSFDDPYSKDGGLAILWGNIAPDGAVVKKGAV
                     LEEMMVHRGPAKVYNSEEECMTAIMGGKVVSGDVVVVRYEGPKGGPGMREMLTPTSAI
                     AGMGLDSSVALITDGRFSGASRGASIGHISPEAAAGGLIGIIEDGDIISIDIPNNKLE
                     LLVDENIIAERRLKFVPLEKPVPNGYLKRYRKMVTSANTGGVFED"
     misc_binding    complement(4660..4905)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5011..6390)
                     /locus_tag="PKH42_05590"
     CDS             complement(5011..6390)
                     /locus_tag="PKH42_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOA19937.1"
                     /translation="MNENLLSGIYKNNEEMNVNFDEQGKKIVVLDDDPTGTQTVYDIP
                     IYTRVDYEIIQKIFQDNNIKAFYILTNTRSMSKEKVMELDSKIARNIDLVSKRTGIDY
                     ELIVRGDSTLRGHFPLELNIIKDNISKKIDGYFLIPFFQEGGRITVDDVHYVKENNTF
                     IPVGETQFARDHTFGFTSSNLKDYVEEKTNCLVLKDQVVSISLEELRSNSSAVFNKIM
                     DLNGRICIVNAENYSDIQAFVNGLIKAEYQGKNFLFRTAASFVKSRIGLKDNGLLDGN
                     TLNIIDNRGGLIVAGSYVNKTSQQINHLMKETSIYSIEIDVLSILTESDNSNIIKKIS
                     QKIDENIIRGIDTLIYTSRSVILGNDNTPNLDIGNKISLYLAKIVKQLKEAPAFIVAK
                     GGITSSDIATEGLNIEEAIVCGQIANGVPVWKSGRESKFYGLVYVVFPGNVGDEDELT
                     NVIRRLHKK"
     gene            complement(6458..7282)
                     /locus_tag="PKH42_05595"
     CDS             complement(6458..7282)
                     /locus_tag="PKH42_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOA19938.1"
                     /translation="MRKFKVALIQTMVYDDKEKNVKNAVKMIGKVSKEGADIAVLPEM
                     FCCPYNTSYFRKYGEEEGGYAYKELSKAANSSGIYVVAGTIPELERTHVYNTSYVFDR
                     KGKRVAKHRKMHLFDIDVEGGQYFKESDVLSPGRDVTVFDTEFCKIGLGICYDIRFPE
                     LSRLMVLEGAEVIIFPAAFNMTTGPAHWELNFRGRALDNQVYTVGVAPARDMESSYHS
                     YGNSIVVSPWGNVINRMDEKEGYIIQEIDLEYVKKVRNELPLLKHLRRDLYTLSKN"
     gene            complement(7352..7981)
                     /locus_tag="PKH42_05600"
     CDS             complement(7352..7981)
                     /locus_tag="PKH42_05600"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOA19939.1"
                     /translation="MTKIYLTRHGETVWNRQCRFQGHKNSDLTEKGILAAELLADRIE
                     EIELDYIVSSPLMRAYNTAEIIRGNKNIQIIKHDGLKEINLGKFEGMSYKDIQKNYSK
                     LLAEIEKDPFNNRYPNGENLQEFYKRVVKAFNEVIDKYRNKSTLIVAHGGTIKCIEAY
                     IRKFKLSSDWMGNVVKNCSLSYLEIDDNNEIKEIFYNDTKHLEGSAALN"
     gene            complement(8004..9971)
                     /gene="ligA"
                     /locus_tag="PKH42_05605"
     CDS             complement(8004..9971)
                     /gene="ligA"
                     /locus_tag="PKH42_05605"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOA19940.1"
                     /translation="MDKKLLLMKEKIRLLNEANKAYYQEDREIMSNYEYDKLYDELKK
                     MEEETGIVLSNSPTIRVGYELLSNLPEERHEKVMLSLDKTKEVTALKDWLGEKTGALS
                     WKLDGLTIVMTYEGGRLVKAVTRGNGEVGEVVTNNAKVFTNIPLSIAYKGSLVLRGEA
                     VISYSDFYKINETIPEAAGKYKNPRNLCSGSVRQLNNRITAERNVGFYAFSVVKADEV
                     DFKNSRIEQLLWLNSQGFSIVEFKKVTQENIEETVNWFEENIRENDLPSDGLVLTYDD
                     IKYGESLGSTAKFPKDSIAFKWSDEIKETTLLEIEWSASRTGLINPIAVFEPVELEGT
                     TVSRASVHNLSIMEDLELGIGDRINVYKANMIIPQISDNLTRSNNIEIPEHCPVCGGE
                     TSIKNDNDIKTLYCINDECLAKQIKSFTHFVSRDALNIKGLSEATIEKLIAKGLIKEL
                     ADIFHIERYKDIITEMEGFGEKSFNNLVNSVNKARKTTAARLLYSFGITNIGLSNAKL
                     ISRKFGDDWEKIQNAKYEELIEISGIGEVMAKNYVKFFSDEKKKVIVEDVLKEVEIET
                     SSKTSSTQIFENYNFAITGAVEHFKNRDELKAAIEERGGKVTSSVTSKTNYLINNDNL
                     SNSSKNKKAKELGIEIITEEQFLKWMNTGVR"
     gene            complement(9986..12193)
                     /locus_tag="PKH42_05610"
     CDS             complement(9986..12193)
                     /locus_tag="PKH42_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOA19941.1"
                     /translation="MEILNSLNDKQREAASHDKGPILVIAGAGTGKTRVLTHRTAYLI
                     ESGKAMPWEVLAITFTNKAASEMKERIGRLIDYSIDRMWIGTFHSVCVRILRSFIDRI
                     GYDKNFIIYDDDDQKIVIKDCIKELELDPKLYNYNSIKALISNEKNRRNTPDNYIKDN
                     YLNYANRNKGEVYALYEKKLKAANALDFDDLILKTLELFEKNEDILKHYQERFKYILV
                     DEYQDTNNAQYNLIRILGKKKNGESNVFVVGDEDQSIYGWRGADISNILNFENDFKGA
                     KIVKLEKNYRSTDVILNAANGVIKNNYQRKGKNLYTEQKKGNLIRIIETDNERDEAFT
                     TAALIRKEHRENNINYSEIAILYRTNAQSRALEEGLIREGIPYKIVGGVKFYDRKEIK
                     DIMAYLRLILNQKDDVSFERIINVPRRKLGQKSTDTISNYAEANGVSKFEACFDFDKM
                     GLIPSAAKSIESFVSMMEMLMIKKDAMPLSEFIISVIEDTGLKDMLLQDETVEGRSRV
                     ENIDEFLSAAKDFEERYEENTLEDFLAHISLLADIDKTDSIKDVVTLMTVHSAKGLEF
                     DTVFITGLEDGMFPSIHFDGEDNVEEERRLFYVAITRAKRMLYITHARDRIRFGSHEM
                     RMKSRFLEEIPEECIEESDPYSYANKKLEQLESQIARKTSFFEYRAEITEKEKPKIKS
                     DKFEPGDKIMHKSWGKGTVVQIKDSGDDKIIVAAFENKGIRNLSLKLAPIEKI"
     gene            12362..12802
                     /locus_tag="PKH42_05615"
     CDS             12362..12802
                     /locus_tag="PKH42_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA19942.1"
                     /translation="MKKILVPVDGSAASQKAVAQAFDIGRKYNSDVVVFHVFIEHDID
                     MYNKFGVVIDPDMNKLKKRLKENESKMLDSIINQYDNTGINLKKKIVSGVAYEEILKE
                     AKDGNYDLIVIGQRGYSKIKRFFVGSQTVRVISDATCPVLVVHE"
     gene            12819..13271
                     /locus_tag="PKH42_05620"
     CDS             12819..13271
                     /locus_tag="PKH42_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOA19943.1"
                     /translation="MKKILIPIDGSPLSVKIGEKAVELGKLIDAELIFITVINMPSED
                     KYAYFGMTVENQFSANRKEMLNKLMQQESKMLNIIVRNLDTGNLKVTQKLLVGRPAAE
                     ILKFAEEENINYIFMGRRGFSPVQRFFVGSVTQKVISAAPCPVIVING"
     gene            complement(13315..14304)
                     /locus_tag="PKH42_05625"
     CDS             complement(13315..14304)
                     /locus_tag="PKH42_05625"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOA19944.1"
                     /translation="MKYIENNSTDPHYNLAFEEYVFKNLDNNEDFVLLWRNGPSIIVG
                     KNQNTIEEINMEFVKNNNINVVRRVTGGGAVYHDLGNLNFSFITKAQSNLIDFKTYNI
                     PIVKSLEKLGVDCELSGRNDIVIDGKKFSGIAQSIIKGRVLNHGTLLFDSKLDILSKA
                     LNVKRDKIESKGVKSVSSRVTNIKPYVKDDIDVLQFKQILLKNIFEYFNQPVEIFELS
                     DEDRNKIQKMVDERYGTWEWNFGRSPEFNYKGYDRFEGGGVEVRLHVCNGIIESCKIY
                     GDFFGKGDVKNLEDKLLGVKYDINEINKAIEDISIDEYLGRITKEEFLRCLFS"
BASE COUNT         4128 a         3002 c         2163 g         5102 t
ORIGIN      
        1 ttatagtatt aatgacggta tgaaaagcgg cagataaatg taaatgaaaa atactattat
       61 caagcctata agatatggaa caaccttttt agctattctt tcaattggca ttcctgttat
      121 tgaagatgcc acaaaaaggt cgatggcaac aggcggcgta atcattccga ttgcaatgcc
      181 tattgtgaat atcaaaccaa agtgaataag gtcgatattt agcattctta caagtggaag
      241 aaataccgga gtaataagta tgatagccga ggatgtttcc atgaaaacac cggcaatcaa
      301 tactatcaca gccatcaaga gcagtataat atatttgttg tctgagactg aaagcactgc
      361 ttgagcaact gcctcgggta tctgccagtt ggcaagagcc catccgaaaa caccgctgca
      421 tgctattatg gtcataatga ttgcagaggt cttgccgcta ccaagcataa tctgtccaag
      481 ctctttaaga gtcaaatcct tgtaaataaa cattgacgca aacatggaat atacaactgc
      541 aacaccggcg gcttcagatg gagtaaaata tcccgaaaat atcccgccta gtataataag
      601 cggcattatc aatgcccaaa cggctttcct gaaagtttca accatatatt tgaattcaaa
      661 tgcccttccg tccggatagt tgaatttttt agcctcataa agagaaattg ctattaatat
      721 accccccatc atgaagccgg gaaaaaaacc atttttgaat aatctggtta cactttgctc
      781 ggctattaca gcatatagaa ccatgggaac agacggagga atgactactc cgatacatcc
      841 tcccgaagct attaaagcgg ccgcacgatc tacctcatac cctcttttct caagttcagg
      901 cagcatagat gttccaactg ctgcagtggt agcagcgcct gatccggata tagcagcaaa
      961 gaacattgat gccaatacag aaacagccga cagtcctcct ttaactttgc ctatacatgc
     1021 ttctgcaaat gcaaccagca tttttgacat ttctcctctg gcaagcaaat cccctgccaa
     1081 aataaagaag ggaattgcaa ccaatgaaaa tgaatctgag gaagcaaata agcgctgtac
     1141 tactatcaat aggtttacat cacctactat cagtacgact acaccggcaa cagcaacaga
     1201 aaatgcaatg ggaacaccta aggcaagaca tcctaaaaag gttaaaagta gtatactacc
     1261 cattattctg ccacctccct tgtaactaaa atttcaaaaa ttgcatttag ggcgtgaagc
     1321 agcattactc cgcatccgag tggaattgcc atatacatta aattcattct tatgcctaag
     1381 gcaggtgagc tttgcgctcc ttgtttcatc atgtatatta cgccataata aaccgcaata
     1441 atgaaaaatg caatgcaaat caggtgtaca agtatctttg catatttatt tgccttaccc
     1501 ttaaatttat cctgaacgaa ggacacatta atgtgccctc ctcttttata aacacatcct
     1561 gctcctatga aggttgacca gacaagcaaa tatcttgtta actcttcact ccaagcaagg
     1621 gcagtgaaaa ataccctggc tattatttga atacttgaaa agaataccat ccctgccata
     1681 gtcaatatta agagactgca gcctatctta tcaataaagt cgcttgcctt ttttataacc
     1741 ttagcaatga caaccacctc ctatatagtt actgacttaa ttacttaatt gcttctttaa
     1801 ttcttgtcag gtattcgctg tattgcggat attgttcgta taccgcctgt accgcttgtc
     1861 tgaagctttc caaatccggt tcttcaataa gctccatacc gtttgcttta aggtctgcaa
     1921 tttgagcttc ttcattttct gcaacccatg ctctttcgta ttcagccgct tcctgggctg
     1981 cttgtacaaa tatctcctgt acatcagccg gaaggctgtt aaatttttct tcgctgaatg
     2041 ttataacagc tgctgcatat gcatgtcttg tcatagtgac atgtttttgt gaatcccaca
     2101 ttttgtatgc atgaataaca ttaaccgggt tttcctgtcc ttgtattgta ccttgttgca
     2161 aagcggtaag tgcttcagtc catgccatag gcacggcatt tacatccatt gccttaaatg
     2221 cagataaata aacttcattt tccatcacac gtatttttaa atccttaata tcttcagcag
     2281 tgcgtacagg tcgcacactg ttagtcaaat tacggaaacc tctttccgcg tatgcaagtc
     2341 ccttaaggtc gtgatcttca agcttagaga gaatttcttt tcctatttca ccgtcaagta
     2401 ccttataagc agaagcgttg tcagggaata agaagggaag gtctaaaaca ccgaattcag
     2461 gtacaaaatt tacaaacggt ccgcttgtaa tgataccgga atcaagggtt cccatctgca
     2521 tactttcaag cattgttctt tcatcgccca atgatgaatt ggggaacaac tgaatgtcat
     2581 atgcaccgtt ggttctttcc tctacaagtt ctgaaaattt tgtggcacat acgtaaaagc
     2641 tgtcttgttc attgattgtc atacctaatt taataaccgt cggttcagcc gatgtcggcg
     2701 gattatcaac agtttgtccg catgctgtac ctaagaccat aacaactatt aatgctaatg
     2761 ctataaaatt taattttttc atttttttct ccttatatat tcataatttt tttattaatc
     2821 tcttaatctt caaatactcc tccggtattt gccgaagtta ccatcttacg gtatcttttt
     2881 aaatatccgt ttggtaccgg tttttccaaa ggtacaaact ttaagcgtct ttctgctatt
     2941 atgttttcat caaccaaaag ctcaagctta ttattaggaa tgtcgatgct aattatatct
     3001 ccatcctcaa tgatacctat caatcctcct gctgcagctt caggagatat gtgaccaata
     3061 gaagctcctc ttgatgctcc cgaaaatctg ccgtctgtta taagtgcaac gcttgagtca
     3121 agtcccattc ctgcaattgc agatgttggt gtaagcattt cacgcatgcc cggtccacct
     3181 ttaggtcctt cataacgaac tacgactaca tcacctgaaa caactttacc gcccattatg
     3241 gctgtcatgc attcttcttc actgttgtaa accttggcag gacccctgtg aaccatcatt
     3301 tcttctaaaa cggctccctt ctttacaact gctccgtccg gtgctatgtt tccccataat
     3361 atggctaatc cgccatcctt tgagtatgga tcatcaaagc ttcttataac tgaattatcc
     3421 tttatataag catccttgat gttttcgcct acggtatttg ccgtaacagt aatacaatcc
     3481 gtattcagca gtccatgatt gtcgagttcc ttcagtacag acgggattcc tcctgcctgc
     3541 cataaatcaa ccagacaatc ttcccctgca ggacttaatt ttaccagatg aggagttgcc
     3601 ttgcttatat tgtttatttc gttaaggtcg atttttagtc ctgcttcatg tgctattgca
     3661 ggtaagtgca gtacagtatt tgttgagcat cccaaagcca tatctgctgt taaagcatta
     3721 tgaagggaat caagggtaac aatatcgcga ggacggatat ctttttccaa aagctccatt
     3781 accttcatgc ctgcttcttt tgcaaggcgt atacgggctg catgaacagc gggaatagtg
     3841 ccgtttccgg acaagccaag tccgataacc tctgtcatgc aattcatcga attagcagta
     3901 aacatacctg cacatgagcc gcaaccggga catgaataaa gctctatttc ctttatccgc
     3961 tcatcactca ttttttttgc accggctgct ccaacagctt caaatgagtt tgagacagtt
     4021 agtttcttgc cgtcataata tccgggcatc atgggaccgc cgcttacaaa aatacttgga
     4081 atattaagcc ttaatgctgc cataatcata ccgggaacta ttttatcgca gttcggaaca
     4141 aaaacaagtg catcaaaggg atgagccatt gccattattt caactgaatc cgcaatatgt
     4201 tcacggctta taagagagta tttcatacct tcgtgaccca ttgcaatacc gtcgcatact
     4261 cctattgcag gaaatacaaa gggtgttccg cctgccatca ttactcctga ttttacagcc
     4321 tcaacaatat catccaagtg caaatgtccc ggaattatat cattctgagc atttacgatg
     4381 ccaaccatag gttgtctgat ttctttttct gtgagcccca gagcgttgaa taaggctctg
     4441 tgagcaatcc tttccacgcc tgatttcatt tgatcacttc tcataaattt cctccaaata
     4501 aaaatccatt ttcttactta ctttaaaatt gattattctg ccccctgttt tacctcctct
     4561 tattttttga tttttgtttt tattgaccgg aatgaagtta atctcccgaa gggttaggcc
     4621 tataaaaaaa cctctcatcc ctacatacat ctagatgtat atagggacga gaggtctttc
     4681 gcggtaccac cctaattcac gataaaaaaa tatcgcctca ttcaagtcga aaaaacaaaa
     4741 aagttttttg aactctattc ttgtaacgca gaaatacgtc ctcgtctact taattcgact
     4801 cggaagctca ggagtgatca ttatatctca taacaccgat tcccaccaac catcggctct
     4861 ctgaagttat tttccggata tttttgtctc cgtcaaagct ttaatcaatt ttttcatatt
     4921 atatacacag gatattgttt tgtcaaccat tttttaaaaa gaaaaatacc ggtctttcat
     4981 cgtacttatt tatcatgtag ggggaaattt ttatttttta tgaagtctcc taatgacatt
     5041 tgttaattca tcttcatctc caacattgcc tggaaagact acataaacca atccgtaaaa
     5101 tttactctcc ctgccagatt tccacacggg tacgccatta gctatttgtc cgcaaactat
     5161 cgcttcctca atattcaacc cttcagtcgc aatatcactt gatgtgattc cgccctttgc
     5221 aacaataaaa gccggggctt cttttaactg ttttacaatc tttgcaagat aaagagatat
     5281 tttattacca atatctaaat taggggtgtt atcatttcca agtattacag aacgtgaagt
     5341 gtaaatcaag gtatcaattc ctctaataat attttcatca attttttgag aaattttttt
     5401 aattatatta ctgttatctg attctgtaag aatagacaga acatcaattt ctatgctgta
     5461 aatagaagtc tctttcatca gatgattaat ctgttgacta gttttgttta cataggaccc
     5521 ggcaactatc aaacctcctc tgttgtcaat tatgtttaac gtattcccat ctaacaaacc
     5581 attatccttt aaaccaatcc ttgacttaac aaaagaagca gctgttctaa agaggaaatt
     5641 tttcccttga tattcggctt ttatgagtcc gtttacaaat gcttgaatat cactatagtt
     5701 ttctgcatta acaatacata ttcttccgtt caaatccatt attttattaa aaactgcact
     5761 gctgttgctt cttaattctt ctaaacttat tgatacaact tggtctttta atactagaca
     5821 atttgttttt tcttctacat aatcttttaa gtttgatgaa gtaaaaccaa atgtatgatc
     5881 tcttgcaaac tgtgtttctc ctacaggaat aaatgtatta ttttctttaa cataatggac
     5941 atcatcaaca gtaattctcc cgccttcttg aaaaaatggt atcaaaaaat atccatctat
     6001 tttttttgat atattatcct ttattatatt taattctagt gggaaatgtc ctctgagagt
     6061 tgagtctcct cttacaatta attcataatc tatacctgtt ctcttagata ccaaatcaat
     6121 attcctggca attttggagt cgagttccat tactttttct tttgacattg atctggtatt
     6181 agttaaaata tagaatgctt taatattatt atcctgaaat attttttgaa ttatttcata
     6241 atctactctt gtatatatag gtatatcata aaccgtttgg gtacctgtcg gatcatcatc
     6301 taaaactact atctttttac cttgctcatc aaagtttaca ttcatttctt cgttattttt
     6361 ataaattcct gacaataaat tttcattcat aacaatctcc aattccacag ataaatagtc
     6421 tgtagtaaat tgcccaaacc attaatttac aatataatta gtttttgctt aatgtatata
     6481 agtctcttct taaatgcttt aatagaggca gttcatttct taccttcttt acatattcaa
     6541 ggtcaatttc ctgtattata tacccttctt tttcatccat tctgtttatc acattgcccc
     6601 atggtgagac tactatggaa ttaccatagg agtgatagga tgattccata tctctggcag
     6661 gagcaactcc tactgtgtac acctggttat caagggctct tcctctaaaa tttaactccc
     6721 aatgagcagg accagtggtc atgttaaatg cggcaggaaa aataataacc tcagctcctt
     6781 ccaataccat gagcctgctt aattcaggaa atcttatatc ataacatata cctaaaccga
     6841 ttttgcaaaa ttccgtatca aagactgtta cgtctcttcc gggagagaga acatccgatt
     6901 ctttgaaata ttgtcctcct tcaacatcaa tatcgaataa atgcattttc ctgtgcttgg
     6961 caactctttt gccttttctg tcaaacacat atgatgtatt gtaaacatga gttctttcaa
     7021 gctcgggtat tgttccggca acgacataaa tacctgaact gtttgctgct ttacttaatt
     7081 ctttataagc atacccgcct tcttcttcac catattttct aaaatatgaa gtgttataag
     7141 ggcagcagaa catctccggt aacactgcaa tatcggcgcc ttcctttgac actttaccaa
     7201 tcattttcac agcattttta acattttttt ctttatcgtc ataaaccatg gtttgaatta
     7261 atgcaacttt gaattttctc atagcaccct cctcttataa atacttgcca attttctacc
     7321 ttattctgtt tttcccggga aatttcgaca attaattaag tgctgcgctt ccttccaaat
     7381 gttttgtatc attatagaat atttctttta tttcattatt atcatctatt tctaaataag
     7441 aaaggctgca attcttaact acatttccca tccagtcact gcttaactta aattttctta
     7501 tataagcttc gatacattta atggtacctc catgggcaac tattaaggtt gacttgtttc
     7561 tgtatttatc tattacttca ttaaatgcct tcacaaccct tttgtaaaat tcctgaaggt
     7621 tttctccgtt tggataacgg ttgttgaaag ggtccttttc aatttcggca agcagcttag
     7681 aataattttt ttggatatcc ttatagctca ttccttcgaa tttgcctaaa tttatttctt
     7741 ttaaaccatc atgttttata atttgtatat tcttattccc gcgaattatt tcagcagtat
     7801 tgtaagctct catcaaaggg cttgaaacta tataatccag ctctatctct tcaattctgt
     7861 ctgccaataa ttctgctgcc aatattccct tttccgttaa atccgaattt ttatgtcctt
     7921 gaaacctgca ttgtctgttc cagactgttt cgccatgtct cgttaaatat atttttgtca
     7981 tatcaattcc ctttctatct cgtctatctt acacctgtat tcatccactt aagaaattgc
     8041 tcttccgtta taatttctat tcctaattct ttagcttttt tattcttgga agaatttgat
     8101 aaattatcgt tattgattaa atagtttgtt tttgaagtca cagaagaggt aacctttcct
     8161 cctctttctt caattgcagc ttttaattca tccctgtttt tgaaatgctc aacagcccct
     8221 gtaattgcaa aattataatt ttcaaaaatt tgtgttgatg atgtcttaga gcttgtttct
     8281 atctccactt ctttaagcac atcttctaca ataacttttt tcttttcatc actgaaaaat
     8341 tttacgtaat ttttagccat cacttctccg atgccgctta tttcaatcag ttcttcatat
     8401 tttgcatttt gaattttctc ccagtcatcg ccgaacttcc tgctgattaa tttggcattt
     8461 gacaaaccta tattggtaat tccaaagctg tacaaaagcc tcgctgctgt tgtttttcgc
     8521 gccttattca ctgaattgac cagattattg aaagatttct cgccaaatcc ttccatctcg
     8581 gtaattatat ctttatatct ctcaatatga aaaatatcag ctaattcttt aatcaatccc
     8641 tttgcaatta atttttcaat agtggcttcg gaaagtcctt taatatttaa tgcatctctg
     8701 cttacaaaat gtgtgaatga ttttatttgc tttgctaaac attcgtcatt tatgcagtac
     8761 aatgtcttaa tgtcattatc atttttaatt gatgtttcac cgccgcatac agggcaatgt
     8821 tctggaatct caatattatt gctcctggtt aaattatccg aaatttgagg aattatcata
     8881 tttgccttat acacattgat tctatcgccg attccaagct ccaggtcttc cataatgctt
     8941 agattatgaa cactggcacg gcttacggtt gttccttcaa gctccacggg ctcaaatact
     9001 gcgattggat ttattaatcc cgtccttgag gcactccatt caatttccaa aagagtagtt
     9061 tccttaattt cgtcactcca tttaaaggca attgaatctt tcggaaattt tgcggtactt
     9121 cctaatgatt ctccgtattt tatatcatca tatgttagaa caagtccgtc cgaaggcagg
     9181 tcgttttctc taatgttctc ctcaaaccaa tttactgttt cttcaatatt ttcttgagtt
     9241 actttcttaa attcaactat gctgaatcct tgactgttga gccataatag ctgctcgatt
     9301 cttgaatttt taaaatcgac ttcatccgct ttaactaccg aaaatgcata aaaccccaca
     9361 tttctttctg cggttattct attgtttagc tgccttacgg agccgctgca aagatttctg
     9421 ggatttttat atttacccgc agcttccgga attgtctcat ttattttata aaagtctgaa
     9481 taactgataa ctgcttctcc tcgtaaaacc aaacttcctt tataggcaat actaagagga
     9541 atattagtaa atacctttgc attgttggta actacttctc caacctctcc attacccctg
     9601 gttacagcct ttacaagcct tcctccttca taagtcatca caattgtaag gccatccagc
     9661 ttccacgaaa gggctcctgt tttttctccg agccaatcct ttaaagcagt aacttccttt
     9721 gttttatcca atgaaagcat aaccttctca tgcctttcct caggcaaatt tgataagagc
     9781 tcatatccaa cacggatggt ggggctgtta gacagtacta tccctgtttc ttcttccatt
     9841 tttttaagct catcgtaaag cttgtcatat tcataatttg acattatttc tctgtcttct
     9901 tgataatatg ctttgtttgc ttcatttaaa agccttattt tttctttcat taaaaggagt
     9961 ttcttatcca tactttacct tccgtttaaa ttttttcgat tggagcaagt ttgagtgaaa
    10021 gattccttat ccccttattt tcaaaagctg ccacgattat tttatcgtct ccgctgtcct
    10081 ttatttgtac tactgttccc ttcccccaag acttatgcat tattttatca ccgggctcaa
    10141 acttatctga tttaatcttt ggtttttctt tttccgtgat ttcagctcta tattcaaaaa
    10201 aactggtttt tcttgcgatt tgggactcca attgctcaag ttttttattg gcataagaat
    10261 aagggtcgga ttcttctatg cattcttcgg gaatctcttc caaaaacctt gatttcatcc
    10321 tcatttcatg gcttccaaaa cgaattctgt cccttgcatg agtaatatag agcattcttt
    10381 tggctcttgt tatagcaaca tagaagagtc ttctctcttc ttcaacattg tcttcaccat
    10441 cgaaatgaat gctcggaaac atgccgtctt caagacctgt tatgaaaacc gtgtcaaatt
    10501 caagaccctt agcactgtgt acagtcatca atgttacaac gtcttttata gaatcagtct
    10561 tatctatatc ggcaagcaat gaaatatgag ccaaaaaatc ttctaatgta ttttcctcat
    10621 atctttcttc aaaatccttt gctgcggaca agaattcatc tatattttca acacgacttc
    10681 ttccttcaac tgtttcgtct tgaagtagca tatctttcag ccctgtatct tcgattacgc
    10741 ttattatgaa ttcgcttaaa ggcattgcat cctttttaat catcagcatt tccatcatgg
    10801 acacaaagct ttcaatactc tttgctgctg aaggtataag ccccatctta tcaaagtcaa
    10861 aacatgcttc aaatttcgaa actccatttg cttcagcata atttgaaatg gtatcggttg
    10921 atttttgtcc taattttctt ctcggtacat tgattatcct ttcaaaactc acgtcatcct
    10981 tttgatttag gataagcctt agataagcca ttatatcctt aatttctttt ctatcgtaaa
    11041 attttactcc tcctacaatt ttgtagggta tcccttctct aattaaacct tcttccaagg
    11101 ctcttgactg tgcattcgtc ctgtataaaa ttgcaatttc agagtaattg atattatttt
    11161 ccctatgctc tttgcgaatt aatgctgccg tggtaaaagc ctcatctctt tcattgtctg
    11221 tttctattat tcgtattaag tttccctttt tttgctctgt atacaggttt ttaccctttc
    11281 gttgataatt gtttttaata actccgtttg cagcatttaa aattacatcc gtagagcggt
    11341 aatttttttc aagcttgact attttggctc ctttgaagtc attttcaaaa ttaagaatat
    11401 tgcttatatc ggcacccctc cagccgtaaa tggactggtc ctcatctcct accacaaata
    11461 cattgctttc accgtttttc tttttaccta atattcttat gagattatac tgagcattat
    11521 tggtatcctg gtattcatcc acaagtatat acttaaacct ttcttggtag tgctttaaaa
    11581 tatcttcatt tttttcaaac agttcaagtg tttttagaat taaatcatcg aaatccaatg
    11641 cattggcagc tttaagcttt ttttcgtaaa gagcatatac ttctccctta ttcctgtttg
    11701 cataatttaa gtaattatct ttaatatagt tgtcgggagt attgcgcctg tttttttcat
    11761 tgcttataag tgccttaata ctgttataat tatataactt ggggtctaat tccaattcct
    11821 taatgcagtc ctttattact attttttggt catcatcatc gtaaataata aagtttttat
    11881 cataacctat tctgtcaata aaacttctta atatgcgtac acaaacagag tgaaaagttc
    11941 caatccacat tctgtctatg gaataatcaa taagacgacc tattctttcc ttcatttcac
    12001 ttgctgcttt attggtaaat gttattgcaa gaacttccca aggcattgct tttccggact
    12061 caatcaaata tgccgtccgg tgagtcagga ctcttgtctt cccggtgcct gcccctgcaa
    12121 taaccaatat gggacctttg tcatgagaag ctgcttctct ttgtttgtca tttaatgaat
    12181 ttagtatttc catttttctc ctatctgaaa aagtacgggg acattttttt taattatatc
    12241 acagtcaacc attactctca agattctgat tattgtttta ggaatatatt tgtttatggt
    12301 aaataaattt aggtataagt atattagtta atattctaat tattggctaa ggagggactt
    12361 tatgaaaaaa atattggtac ccgtggatgg ctcagccgct tctcagaaag ctgttgccca
    12421 agcttttgat attggaagaa aatataacag tgatgttgtg gtttttcatg ttttcattga
    12481 acatgacatt gatatgtaca ataagtttgg tgttgtaata gatcctgata tgaacaaact
    12541 aaaaaagaga ttgaaagaaa atgaaagtaa aatgctggat tcaataatta atcagtatga
    12601 caacacaggt attaacctaa agaaaaaaat cgtttccggt gtggcttatg aagaaatttt
    12661 aaaagaagca aaagatggta attatgattt gatagttatc ggtcaaagag gatattcaaa
    12721 gattaaacgc ttctttgtcg gctctcaaac agttcgtgtc atatctgatg caacttgccc
    12781 tgtcttagtg gttcatgaat aaataattgg aggttattat gaaaaaaatt ttaataccta
    12841 tcgacggctc gcccttatct gtaaaaattg gtgaaaaagc cgtagaatta ggcaaattaa
    12901 ttgatgccga attaattttc ataactgtta ttaacatgcc ctcagaagac aaatatgcat
    12961 acttcggtat gaccgttgag aatcaatttt cagcaaatcg caaggaaatg cttaataaat
    13021 taatgcagca ggaatctaaa atgctcaata ttattgtaag aaatttagat accggtaatt
    13081 taaaggtaac tcaaaaatta ttggtaggca ggcctgcggc tgaaatttta aaatttgcag
    13141 aagaagaaaa tattaattat atttttatgg gaagaagagg gttctctccg gttcaaagat
    13201 tttttgtagg ctctgtaact caaaaagtta tttcagcggc accatgtccg gtaatagtaa
    13261 taaacggata attaggaagg gttgggggac ataccttgtc cccgtacttt aaaattagct
    13321 gaatagacat cgcaggaatt cttcctttgt gatcctgcca agatattcat caattgaaat
    13381 atcttctata gctttattta tttcattgat atcatactta acacctaata gcttatcttc
    13441 taaattctta acatctccct tgccgaaaaa atcaccatag attttacagc tttcaataat
    13501 tccattgcac acatgaagtc taacttctac tcctcctcct tcaaatctgt cgtaaccttt
    13561 atagttgaat tccggggacc ttccaaagtt ccattcccaa gttccgtatc tttcatcaac
    13621 cattttttgt atcttatttc tgtcttcatc ggataattca aatatttcaa caggttgatt
    13681 gaagtattca aagatattct ttagaagaat ctgtttgaat tgcagcacat caatatcatc
    13741 cttgacatat ggtttaatat ttgtcactct gctggatacg gactttactc ccttagattc
    13801 tatcttatct ctttttacat ttaatgcttt agacaatatg tccagttttg aatcaaaaag
    13861 cagggttccg tgattcaata ctcttccttt tataatgctt tgtgctatgc ctgaaaattt
    13921 ctttccgtca ataacaatat catttcttcc cgataattca cagtcaactc ccaacttttc
    13981 caatgacttg actatgggaa tattgtatgt tttaaagtct attaaattgc tttgtgcctt
    14041 agttataaaa gaaaaattta aattccccaa atcatgataa acagcaccgc cgccggttac
    14101 tcttctgaca acatttatat tgttgttctt aacaaattcc atattaattt cttctatagt
    14161 attttgattt ttacctacaa ttatggaagg tccgtttctc cataacaaaa cgaaatcttc
    14221 attgttatct aaatttttaa aaacatactc ttcaaatgct aaattataat gtgggtctgt
    14281 cgaattattt tcgatatact tcatgctgca ctccttgtta acataattga aattatgtaa
    14341 atcttatttt gcaaattata taataaagat tatgataatt caatatttag tttaa
//
LOCUS       DAOPUE010000046        14149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4391_length_14149_cov_10.637931, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000046 DAOPUE010000000
VERSION     DAOPUE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_14149_cov_10.637931"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKH42_05630"
     CDS             <1..366
                     /locus_tag="PKH42_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA19945.1"
                     /translation="MLGFIILYSMTLMSINERTLEFSSLRVMGFTKAEIFKMLFKENM
                     VMSALGIIAGIPLGKWLVDYIGFTFNTDIYTLQGIVTAKDIITAIILTIIFIISAQLM
                     TYAKIHKLDFMQALKSRIT"
     gene            380..1555
                     /locus_tag="PKH42_05635"
     CDS             380..1555
                     /locus_tag="PKH42_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOA19946.1"
                     /translation="MKKKKYFIATGVIVIIFIIASLLLGGQTDQPETSKVRLGSIKKT
                     VEETGTVFSKRVNTFYSDMSQRVETLNVSVGDRVKKGDIILVYENNYDLEIERANKQI
                     EAITASYNESVKGADFQEISNLKLNISTIETNLNFARSNLEKIKTLYENNAVSQVEYD
                     EAKNNVTILENQLQEANNNYSKIIQGVSANVKKEYEAQIDEVMIQIKILEKNMEQASI
                     KAEFDGVITELNVHQGSMTQAGVPTVEIQDDKNLGIYVEVLAEDAIEVTKGMAFNIID
                     DDEVIKELQVSRVYPKAQAKISDLGVEQKRVRIEADLDDADYKLGSEVDVEIVLKEKN
                     NVLLVDKDAVYEKDNIKYVTVLSGKNEIETEITTGIEDDKYVEVLSGLSENDTVLIK"
     gene            1603..>2246
                     /locus_tag="PKH42_05640"
     CDS             1603..>2246
                     /locus_tag="PKH42_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008419317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOA19947.1"
                     /translation="MKYDLIIVGAGPSGIFTALELNRENPNKKIMIVEQGKAIEKRNC
                     PKDITKKCISCQPYCNITTGFAGAGAFSDGKLSLSPEVGGDLPELIGYDFTQQLIEYT
                     DSIYLEFGADSRVEGVEAKEEIKEIRRKAIEAGLKLVDCPIRHLGTERAQEIYKTIQK
                     YIIDKGVEIKFNTEAKDILVENGRIKGVVVTDSSKKNSSEEIYADSVVISAGRKG"
     assembly_gap    2247..2346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2347..2820
                     /locus_tag="PKH42_05645"
     CDS             <2347..2820
                     /locus_tag="PKH42_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA19948.1"
                     /translation="SSHNFRNPFNEPIKYGAKVAELLNMLGDGKILVQRYGDILDGKR
                     TWDHELSRSNVRPTLPDAVAGDITSAMPYRAMTNILNFIEALNVVVPGFAGTETLIYA
                     PEVKFYSNKVEISTEFETNIKNLYCLGDASGWTRGLMMSSLMGVRMGQILGKKTN"
     gene            3031..5766
                     /locus_tag="PKH42_05650"
     CDS             3031..5766
                     /locus_tag="PKH42_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOA19949.1"
                     /translation="MEWYNKGKNEVLKELDTNLSVGLSEKEVKRRIEKYGKNEFEEQA
                     KKSILNKFAAQFADFLIIILLIAAGVSAFVGEREDAVIILAIVVLNAALGIYQEGKAE
                     KSVEALQRLSAPNAKVIREGNLTVVPAADIVPGDVVVLEAGDIIPADIRLFESSNLKV
                     EEASLTGESVPVEKDAKAKIVGTVGIGDRHNMGFSSTIVTYGRGKGVVTSTGHNTEIG
                     NIAVKIQTYEEEETPLQIKLNQLGKVLGTLTIVICIIVFIVGMLQGRQPLNMLLTSIS
                     LAVAAIPEGLPAIVTIVLAIGMNRMAEKNAIVKKLLAVETLGATSVICSDKTGTLTQN
                     EMTVVKAYVDDRILYVEGIGYEPKGDVKLNGRSIGISSLPNLRNLISVGVLANDAKLD
                     NSSGFYKVMGDPTEGAIVAFAGKLGQTSEQLNMIYPRIAELPFDSGRKMMTTFHSNYI
                     EGKIVSFTKGAPDIVINKCSKIALNGRIVDFNKELKDKVRSVNTKFARSALRVLSAAY
                     KVWDRLPEHLTFEQVENDMIFVGLVGMIDPPRPEVKDSIKHCRQAGIETVMITGDYKE
                     TAYAIAKELGMANNEGQAIMGEELDKYSDEELREVVKKTKVYARVSPEHKVKIVTALK
                     ENGHITSMTGDGVNDALALKKADIGVAMGITGTDVAKNTAEVILTDDNFATIVNAVEE
                     GRIIFSNIKKFVFFLLSCNIGEILLVFISILLGWNVPLLPIQLLWLNLVTDSLPAMAL
                     GVENAEPGIMKQPPRSTKEGILDGEMLGGIVFQAIAISFASLFAYYWAMRMYGTGSGL
                     IHVRSVVFTTLILSELLRAFSSRSQNYTLFKIGLFTNIRMIQAVFVSFMLTFVVLYIP
                     QLNEIFDVVPLTLRDWQIVTAFSFIPLLVGELYKDLFKNEMPLSNSERKRRISTL"
     gene            complement(5804..7168)
                     /locus_tag="PKH42_05655"
     CDS             complement(5804..7168)
                     /locus_tag="PKH42_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOA19950.1"
                     /translation="MIISPVFDKESSKPLYIQLYEYIKTEITSGLLKPDTKLPSIREA
                     SSTLKLSKTTVENAYSQLVAEGYIDNYPKKGYFVCDLSEYNFDIEATKNTVEYYDEII
                     YLNDGVDKESFDISTWKRLYNKVISDSKTNIYNSCSTQGEEFLREEIANFVNTMRGGH
                     TDSRQVIVGAGVQYLLGMLIGIIKEDYNTVAIEKPGFKKAEFVFEDYMFNIRHIEITD
                     DGFPINELRKSDAEVIYISPSHQYPLGTIMPVNKRMELLDWAYKKNALIIEDDYDSLI
                     RYGGAPIPSLQGLDKNDCVVYLGSFSKMLLPSLRISFMVIPKKLLPKYNEIKDRYTQS
                     TSKIDQLVLANFMKDGYMVKHLRKIKRIYKKKNSYLTSNFRNLFGDRAEVISSDSGLH
                     IVCEANTKKDAQQIIKDAQNSRILINIIRIKKNKVMFSLNYSGVDLNKIHEFTILLGK
                     VLFE"
     gene            7549..8349
                     /gene="pgeF"
                     /locus_tag="PKH42_05660"
     CDS             7549..8349
                     /gene="pgeF"
                     /locus_tag="PKH42_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOA19951.1"
                     /translation="MYKLNKIKDFEYLTFPIFDGYDELFHCFTTRKGGVSKGDFESMN
                     LGFNTGDDEEDVRENYYILSECLNINLEDIVKTFQSHTDNIRYVTEEDKGRVMKKAKY
                     TDIDGLYTDRKNIALMSFHADCNAVFFYDPVKKIIGLSHAGWKGTMTNITGKMIKALK
                     DLGSNPSNLKAAISPSLGQCCFEVDKDVADMFINTDIKYKDFMIKKGEKYHFDLWGIN
                     KYNIIREGMKEENIEVAGMCTKCRNDLFFSHRGQKGKRGLMSGIIMMK"
     gene            8420..9484
                     /locus_tag="PKH42_05665"
     CDS             8420..9484
                     /locus_tag="PKH42_05665"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOA19952.1"
                     /translation="MAHNKIISLILIIFIAVCLTACGTLDMMVGLKDENNPDIADLSD
                     LTDFEIVEDTIGEEALEEPYEEVIETVTISTEEAKMTNIMAYYEDEYGFVVPVNTKIP
                     WEEGIAKATLRSMVRGSETEKRIAQSGLRGVLPEGTEIRGMSIKDGVCRVDFSKNILN
                     TSTLKQEENLISAITYALTEFPTIDKVELLVDGQALSSLTNGYAINKGFERENINLYG
                     SADGVNYTVYYKAPDTEVMGHYVPITFSAAKVGNPVEFVMEKLFEGPPADTTLSNNIP
                     VGINLRDVKVIGDSAVVNLGVEAVNLSEEDYKDMNSIVVLCLEQYGISDVEFNIEGLS
                     FEQAGLYFEDSNVTPVFNQY"
     gene            complement(9576..10583)
                     /locus_tag="PKH42_05670"
     CDS             complement(9576..10583)
                     /locus_tag="PKH42_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA19953.1"
                     /translation="MKKFISLLVVMLLITSLVLTGCNEKPTEYQKVRLIEVTHSIFYT
                     PQYVALEMGLFEKQGLSIELTNGGGADKCMAALVSGEADIAFMGPEASVYVYLQGRDD
                     LAVNFAQLTQRDGSFIVGREKDDNFTIDKLKGHTILGGRKGGMPLMALEYVLKQNGLT
                     PGVDVNVRTDVQFDMMAGAFASGEADYTTLFEPLATSFELQGNGYVVESVGRLAGYIP
                     YTCYSALESYIEKNPEIIQGFSNALYEAMVWVDEHTAMEVAEVIQPQFADTDIDFLAK
                     IVQTYRDLDAWNPDLVLGEDGYNRLIDIMKLAGEIEEGAPYSVIQTPEFAIKAKENYK
                     K"
     gene            10747..11799
                     /locus_tag="PKH42_05675"
     CDS             10747..11799
                     /locus_tag="PKH42_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOA19954.1"
                     /translation="MAISFSDYLMQITDNPMLLITSILTMAVILVNGWTDAPNAIATC
                     VSTRSISAKSAIVMAAIFNFLGVLLMTVINANVAQTIYNMVDFGGNSYDALVALCAAL
                     FAIVIWATAAWWFGIPTSESHALIAGISGAAIALHNGLQGINGSEWIKVLYGLGLSTF
                     LGFTLGWIVVRMVEIIFRSMSRMKANILFTNAQVASGAAMAFMHGAQDGQKFMGVFML
                     GIYLAGGQANVTTFQIPLWMMILCSAIMGIGTSIGGYRIIKSVGMDMCRLQKYQGFSA
                     DMAAATCLFISSMTGIPVSTTHTKTTAIMGVGAARRFSSVNWGIVGEMVMTWILTFPG
                     CGIIGYLMAKLFINIF"
     gene            11811..12428
                     /locus_tag="PKH42_05680"
     CDS             11811..12428
                     /locus_tag="PKH42_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOA19955.1"
                     /translation="MARKTDYFEGFIKLAEYSYNAAKLLDDTLRDFNKDSLQKTMKLM
                     HEIEHTADLEGHEITKKLLKEFITPIEREDIMLLIHKIDDITDCIEDVLLHMYMYNVR
                     VIKEDAIKFSGLILSSCEELIGALKEFKNFKKSKEIRSRIIAVNKIEEEGDNLYTEIV
                     RKLHLTSMNAIDIMTWTIAYNRLEKCCDACEEAANVIESIILKNL"
     gene            12531..13178
                     /locus_tag="PKH42_05685"
     CDS             12531..13178
                     /locus_tag="PKH42_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOA19956.1"
                     /translation="MDKHQKISSAVIRRLPKYYRYLGELNKVGITKTSSRELSEMTGF
                     SASQIRQDLNNFGGFGQQGFGYDVGNLRNEIGKILGLDKKYKIIIVGAGNIGQAIANY
                     TGFYEADYEVVALFDKNPKLVGLSIRNALIMDSDNLEEFLKKEHIDIAVICTPKSVSQ
                     QVAEQLVRCGIRAIWNFAPKDLKMPEEVYVENVHLNESLFSLTYYYNKMKKESGK"
     gene            13401..>14149
                     /locus_tag="PKH42_05690"
     CDS             13401..>14149
                     /locus_tag="PKH42_05690"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOA19957.1"
                     /translation="MAKEVLNPLENSQMQVKKACDALGLHPDVYEILKEPQRVIEVNI
                     PVRMDDGSMKVFKGFRASHNDAVGPAKGGVRFHPGVTLEEVKALSIWMTFKGGVMGLP
                     YGGGKGGVICDPLTLSQGELERISRGYIQKLYKYIGEKEDIPAPDAGTNGQIMAWMVD
                     EYIKLTGGSASLGVITGKPVAWGGSEGRNEATGFGIAVVTREAAKKYGIDLTKAKVAV
                     QGFGNVGRYTVKNIQRQGGTVVAVGEWAPKVG"
BASE COUNT         4802 a         2093 c         2965 g         4189 t
ORIGIN      
        1 atgctcggat ttattatatt atacagcatg actcttatga gtataaatga aagaactttg
       61 gaattttcat ccctgagggt aatgggtttt acaaaagctg aaatatttaa aatgcttttt
      121 aaggaaaaca tggttatgtc agcacttggt ataatagcag gcataccctt aggcaagtgg
      181 ctggtggatt atataggatt tacttttaat accgatatct atactttaca agggattgtt
      241 acagctaagg atataattac tgcaattatt ttaaccataa tatttataat ttcagcacaa
      301 ttaatgactt atgcaaaaat tcataaactt gatttcatgc aggcactgaa aagcaggatt
      361 acttagggtg gaggaaaata tgaagaagaa aaaatatttt atagcaacag gagtaatagt
      421 aattattttc ataatagctt cattattgtt gggagggcag acagaccaac ctgaaacatc
      481 taaggttaga ctgggaagta ttaaaaaaac ggtagaagaa acaggtacag tgttttctaa
      541 aagagttaat accttttatt cggatatgag ccaaagagtt gaaactctaa atgtgtctgt
      601 aggagacagg gtaaaaaagg gtgacataat tttagtttat gaaaacaatt atgatttgga
      661 aattgaaagg gcgaacaagc aaattgaagc aattacggca tcttacaatg aatccgtaaa
      721 gggggcggac tttcaggaaa tttcaaattt aaaactgaac ataagtacaa ttgaaaccaa
      781 tcttaatttt gcaagaagca atcttgaaaa aattaaaact ttatatgaaa ataatgccgt
      841 tagccaagta gaatacgatg aagcaaagaa taatgttaca atattggaaa accagcttca
      901 ggaagcaaat aacaactaca gtaagataat acagggagtt tcagcaaatg taaaaaaaga
      961 atacgaagct caaatagatg aagtgatgat acaaattaaa atactggaaa agaatatgga
     1021 gcaagcttcc ataaaagctg aatttgacgg tgtaattaca gaattaaatg ttcatcaggg
     1081 cagcatgaca caggcagggg tcccgacagt cgaaatacag gatgacaaaa acctcggcat
     1141 ttatgtggaa gtgttagctg aagatgcaat agaagttaca aagggcatgg cttttaatat
     1201 tatagatgat gatgaagtta taaaagaatt acaggtaagc agagtttacc cgaaagctca
     1261 ggcaaaaatt tcagaccttg gcgttgagca aaaacgtgtg agaattgagg cggacttgga
     1321 tgatgcagac tataagctgg gctcggaggt tgatgtagaa atagtactta aggagaagaa
     1381 taatgtacta ctggtagata aggacgccgt ttatgaaaag gataatataa aatacgtgac
     1441 tgttttaagc gggaaaaatg aaattgaaac cgaaataaca accggaattg aggatgacaa
     1501 atatgtggaa gtactatcgg gattaagcga gaatgacaca gtcttaataa aataaaggaa
     1561 tgtaacacac tttcgaatgt actcgttgga gggagaaaga ttatgaaata tgatttgatt
     1621 atagtagggg caggcccgtc agggatattt acagccttgg agttaaacag agaaaaccct
     1681 aataaaaaaa ttatgatagt tgaacaggga aaggcaattg aaaagagaaa ttgtccgaag
     1741 gatataacaa aaaaatgtat cagctgtcaa ccttattgca atattacaac gggctttgcg
     1801 ggagcaggtg cattttctga cggcaagctg tctttaagcc cggaggttgg tggtgacttg
     1861 cctgaattaa taggatatga ttttacacag cagttaattg aatacacaga ttcgatttat
     1921 cttgaatttg gtgctgatag ccgggtagaa ggtgttgaag ccaaggaaga aattaaggag
     1981 ataagaagaa aagctattga agccggactc aagcttgtgg attgtcccat aagacatttg
     2041 gggacggaaa gagctcagga aatttataaa acaattcaaa aatatataat tgacaaaggc
     2101 gttgaaatta aattcaacac tgaggctaaa gatatattag ttgaaaatgg caggattaaa
     2161 ggtgttgtgg ttaccgactc gtcaaagaaa aatagcagtg aagaaattta tgcggattct
     2221 gttgttattt cggcgggcag aaaaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnagct cgcataattt caggaatcct tttaatgagc cgattaagta tggtgcaaag
     2401 gttgcagagc ttttaaacat gcttggagac ggaaaaattt tggttcaaag atacggtgat
     2461 atactggacg gcaaaagaac atgggatcat gaactgtcaa ggtcaaatgt cagaccgaca
     2521 ttgccggacg cggtagcggg agatattact tcagctatgc catatagagc catgacgaat
     2581 attttaaact ttatagaagc attaaatgtt gtggtgccgg gatttgccgg aacggaaact
     2641 cttatttacg cgcctgaagt aaaattttac agcaacaagg ttgaaataag tactgaattt
     2701 gaaacaaata tcaaaaattt atattgtttg ggtgatgcaa gcggctggac gagaggtttg
     2761 atgatgtcat cattgatggg agtacgaatg ggacagatat tagggaagaa aacaaattaa
     2821 tagcatatgg ggacaaatta aaacaggtat ggggacacac ttctactata gggtaaatct
     2881 ttgaagtaag aggagtgtcc ccaacttatt tttttgacaa attcattggg caatcattaa
     2941 ggattgtcca taaatttgga ataaattata aatttgccaa tatattatac tagtgttgac
     3001 aacacaaatt attacaggag gaaaaataat ttggaatggt ataacaaagg caaaaatgaa
     3061 gttttaaaag aactagacac aaatttaagt gttgggttaa gtgaaaagga agttaaaaga
     3121 agaattgaaa agtatggcaa aaatgaattt gaagaacagg cgaaaaaaag tatattaaat
     3181 aagtttgcag ctcagtttgc agatttctta atcattatcc tgctcatagc agcaggagta
     3241 tctgcgtttg taggggaaag agaagacgct gtaataattt tggcaattgt agttttaaat
     3301 gctgctcttg gaatctatca ggagggaaag gcggaaaagt cggtagaagc tttgcaaagg
     3361 cttagcgcac ccaatgcaaa ggttataagg gaaggcaatt taaccgtggt tcctgctgca
     3421 gacattgttc cgggagatgt agtagtgttg gaggcaggag atataattcc tgctgacata
     3481 agactctttg aaagctcaaa tttaaaggtt gaagaagctt ctttaacagg agaatcagtt
     3541 cctgtggaga aggatgcaaa agcaaaaata gttggtaccg tcggaatagg ggacaggcat
     3601 aatatgggct tctccagcac aatcgtaacc tacggtaggg gcaagggtgt agtaacatct
     3661 acgggacaca atacggaaat cggtaatatt gctgtaaaaa tacaaactta cgaagaagaa
     3721 gaaactcccc tgcaaataaa gttaaatcag cttggcaagg ttcttggaac actgacaatt
     3781 gttatttgca taatagtatt tatagtgggc atgcttcaag gaagacaacc cttaaatatg
     3841 cttttgacat caataagtct tgcggtagca gcaataccgg aggggttgcc tgcaattgtt
     3901 acaattgtat tagccattgg aatgaacaga atggcagaaa aaaatgcaat tgtaaaaaaa
     3961 cttctggcgg ttgaaacact gggagctaca tctgtaatat gttcagacaa gaccggtacc
     4021 ttgacacaaa atgaaatgac agttgtgaaa gcatatgttg atgacagaat tttgtatgtt
     4081 gaaggtatcg gatacgagcc aaaaggagat gtgaagctca acggaaggag tataggtata
     4141 agttcgcttc ctaatttaag aaatttaata tccgtaggtg ttttagcaaa tgatgcaaaa
     4201 cttgataatt catcaggctt ttataaagta atgggtgacc ctactgaagg agcaatagta
     4261 gcttttgcgg gaaagttagg acaaacatca gagcagctta atatgattta tccgagaatt
     4321 gccgagcttc cctttgattc gggaaggaag atgatgacaa ctttccactc aaactatatt
     4381 gaaggaaaaa ttgtttcatt cacaaagggt gctcccgata tagttataaa taaatgcagt
     4441 aaaattgcac ttaacggcag aatcgttgat ttcaacaaag agttaaaaga taaggtaaga
     4501 tctgtaaata ctaagtttgc acggtcagcc ctaagagttt tgtcagccgc atacaaggta
     4561 tgggacagac tgcctgagca tctcacattt gaacaggttg aaaatgacat gatatttgtg
     4621 ggtttagtcg gaatgattga ccctcccaga cctgaggtaa aagattccat taagcattgc
     4681 agacaagcgg gaattgaaac agtaatgatt acaggggatt acaaggaaac agcatatgcc
     4741 attgcaaagg aattgggaat ggccaataat gaaggacagg caataatggg tgaggagtta
     4801 gataaatatt cagatgaaga attaagggaa gttgtaaaga aaaccaaagt ttatgccaga
     4861 gtatcacctg agcacaaagt taaaatagta actgctttaa aggaaaacgg acatattaca
     4921 tctatgacgg gggatggagt caatgacgct cttgctctta aaaaggcgga catcggcgtt
     4981 gctatgggaa ttaccggtac ggatgtggca aaaaacactg cagaagttat acttacggat
     5041 gataattttg caaccatagt aaatgccgtg gaagaaggaa gaataatttt ttctaatatt
     5101 aagaaatttg tattcttttt attaagctgc aatataggtg aaatccttct cgtgtttata
     5161 agtattctat tgggttggaa tgttccactt cttccgatac agcttttgtg gctgaatctt
     5221 gttacagaca gccttcctgc catggcatta ggggttgaaa atgcagaacc gggtattatg
     5281 aaacagcctc ccagaagtac aaaggaggga atacttgatg gagaaatgct tggaggaatt
     5341 gtattccagg caattgccat atcattcgct tcgctctttg cgtattattg ggcaatgagg
     5401 atgtacggta ccggaagcgg cttaatacac gtacgctcgg tagtttttac aaccttgatt
     5461 ttgtcagaat tgttgagagc tttttcttca aggtctcaga attatacctt gtttaaaata
     5521 ggacttttta ctaacataag aatgattcag gcggtctttg tatccttcat gcttacgttt
     5581 gtggttttat acatacctca attgaatgaa atatttgatg tagtccctct tactcttagg
     5641 gattggcaga ttgtaacggc attttcattc atacccttgt tggtaggcga attgtacaag
     5701 gatttgttta agaacgagat gcccctaagt aattctgaac gtaaacgaag aatctcaaca
     5761 ctctagatat taggacaaga ttatcctgac gacagcgaat ttctcattcg aacaacactt
     5821 ttcctaatag gatggtgaat tcatgtattt tatttaagtc cacaccgctg tagtttaagg
     5881 aaaacataac cttgtttttc ttgattcgga taatgtttat aaggatgcgt gagttttgcg
     5941 catcttttat tatttgctgc gcatccttct ttgtattggc ttcacaaaca atatgaagac
     6001 cggaatcaga acttataact tctgccctat ctccaaaaag gtttctaaaa tttgatgtta
     6061 gataagaatt cttcttttta tatatacgtt taattttacg gaggtgttta accatatatc
     6121 cgtctttcat aaagtttgca agaactagct ggtcgatttt tgaagttgat tgtgtatagc
     6181 ggtcctttat ttcattatac ttaggcagaa gtttttttgg tattaccatg aagctgattc
     6241 ttagagatgg gagcagcatt tttgaaaatg agcctaagta gactacacaa tcatttttgt
     6301 caagtccttg aagacttggt atgggcgcac ctccatacct aatcaaggaa tcataatcat
     6361 cctcgataat gagtgcgttt ttcttgtaag cccaatccag caattccatt cgtttattga
     6421 caggcattat ggttccaaga ggatattggt gagagggact gatgtatatt acttcagcat
     6481 cacttttcct tagctcgtta attggaaagc cgtcatcagt aatttcaata tgtcttatgt
     6541 tgaacatgta gtcttcaaaa acaaattctg cttttttaaa tcccggtttt tcaattgcaa
     6601 cagtattgta atcctctttt ataattccta tcaacattcc caataaatat tgaacacccg
     6661 cacccacaat cacctgacgc gaatctgtat gaccgccgcg catcgtattt acgaaatttg
     6721 caatttcttc tcttaaaaat tcctcaccct gagttgaaca gctgttgtaa atatttgttt
     6781 tactatcaga aatcaccttg ttgtaaagtc ttttccaagt tgaaatgtca aagctttctt
     6841 tatcaacgcc atcatttaaa tatattattt cgtcatagta ttccactgta tttttggttg
     6901 cttcaatatc aaaattatat tcgctcaaat cgcatacaaa gtatcccttt ttcggataat
     6961 tatctatata accttctgcc actaattggc tgtatgcatt ttctacagtt gttttgctca
     7021 attttaatgt ggaggaagct tccctgattg aaggcagctt cgtatcaggc tttaataaac
     7081 cggaagttat ttctgtctta atatattcat ataattgtat gtataacggt ttgctgctct
     7141 ccttatcaaa aacaggcgaa ataatcatat ggcctccttg gctgtcttgg ttgattttta
     7201 ttattttagc taaatataaa ggtacacaat tattttaaca tatgtttata taaaatcata
     7261 taaaaatttt atacaggtat ggtataataa atgaaatgtt ttcttgaata aagagaaaaa
     7321 taataaaata tgtagcgaac atttgcagga cacgattata attcttagcg aatgcaggtt
     7381 aaaaattcgt taagcagctt gcactgtttg agcggagcga gtttgcaagc tgtaggattt
     7441 ttagccggaa tgagctttag aattattcgt ggccgaaacc gagagcggat attttattat
     7501 tttacatagt atatgaattc cataatgaac ttgaagagag gaattattat gtataaatta
     7561 aataaaataa aagattttga atatttgact tttccgattt ttgatggata tgatgaacta
     7621 tttcactgct ttaccacaag aaaaggcgga gtcagcaaag gtgattttga atccatgaat
     7681 ttaggtttta atacaggcga tgatgaagaa gatgtaaggg aaaattatta tatattgtct
     7741 gaatgcctaa atattaattt agaggatatt gtcaaaacat ttcaatctca taccgataat
     7801 atcagatatg taacggaaga agataagggc agagttatga agaaagctaa atacactgat
     7861 atagacggtc tttatacaga caggaaaaat attgcattga tgtcttttca tgcagattgc
     7921 aatgcagtat tcttttacga cccggttaaa aaaataatcg gactaagtca tgcaggatgg
     7981 aaaggaacaa tgacaaatat tacgggaaaa atgattaagg cattaaaaga tcttgggagc
     8041 aatccttcaa acttaaaggc agccataagc ccttctttgg ggcagtgttg ttttgaagtt
     8101 gataaggatg ttgcagatat gtttataaac acagatatta aatacaaaga ttttatgata
     8161 aaaaagggag aaaagtatca ttttgatttg tggggaatca ataaatataa tataataaga
     8221 gaaggaatga aagaagaaaa catagaggtt gcaggtatgt gtaccaaatg ccgcaatgat
     8281 ttgttctttt cccacagagg tcaaaaagga aaaaggggtc tcatgtcagg aataataatg
     8341 atgaaataat tttttaaagt catactttcg gtacatgcta aatataataa tatatataag
     8401 gcgtgaggag ggtatgaaat tggctcataa taaaataata tctctgatac tgattatttt
     8461 tattgcagta tgcttaactg catgcggcac cttagatatg atggtgggtc tgaaggacga
     8521 aaataatccg gatatagccg atttgtctga tttgacagat tttgaaatcg ttgaagacac
     8581 aattggagaa gaagcattag aagaaccata tgaagaagtc atagaaactg ttacaatttc
     8641 taccgaagaa gctaaaatga caaacataat ggcatattat gaagatgaat acggctttgt
     8701 ggttcctgta aacacaaaaa ttccgtggga agaaggaatt gcaaaagcaa cacttagaag
     8761 tatggttaga ggaagtgaaa ctgaaaaaag aattgcacaa tcaggactac gcggcgtact
     8821 tcccgaaggt accgaaataa ggggcatgtc tatcaaggac ggcgtatgca gagtggactt
     8881 cagcaaaaat attttaaata cttctacctt aaaacaggaa gaaaatttga tttctgcaat
     8941 tacatatgcg cttactgaat tcccaaccat tgataaggtt gaattattgg tagacgggca
     9001 ggcattgtct tcccttacca acgggtatgc aatcaataag ggatttgaaa gagaaaatat
     9061 taatttatac ggctcggcag atggtgtaaa ttataccgtt tattacaagg ctcccgatac
     9121 tgaagtaatg gggcattatg taccaataac attttcagca gctaaggtgg gcaatcctgt
     9181 agaatttgtt atggaaaaat tgtttgaagg acctcctgca gatacaactc tcagcaacaa
     9241 tatacctgtg ggtattaatt taagggatgt aaaagttatc ggggatagtg cagtagttaa
     9301 tctaggtgtt gaagctgtta atttgtcgga agaagactac aaggatatga attcaatagt
     9361 agtgctttgt ttagagcagt atggcataag cgatgttgag ttcaatattg aaggattgtc
     9421 tttcgagcag gcaggattat attttgagga cagtaacgta acacccgtgt ttaaccaata
     9481 ttagcacaaa cgctgacaat caggatgaca gttcatataa tgactgcgtg gagtaagtaa
     9541 aaatccgagg agttattctc ctcggatttt gtatgttatt ttttgtagtt ttcttttgct
     9601 tttattgcga attcaggtgt ctgtattact gaatatggag caccttcttc aatttcacct
     9661 gctaacttca ttatgtcaat aagtctgtta taaccatcct ctccaagcac aaggtcaggg
     9721 ttccaggcat ctaaatccct atatgtctga actatttttg ctaaaaaatc aatatcggta
     9781 tcagcaaatt gaggctgaat tacttcagct acttccattg cagtatgttc gtctacccat
     9841 accattgctt catacaaggc atttgaaaat ccttgaataa tttcaggatt cttttcaata
     9901 taactttcca atgcgctata gcatgtataa gggatatatc ctgctaatct tccaacggat
     9961 tcaacaacat atccgttccc ctgaagttca aatgatgtag ccaacggctc aaacaaagta
    10021 gtgtaatctg cttctccgga agcaaatgct ccagccatca tatcaaattg aacatctgtt
    10081 cttacattta catccacgcc gggagtcaat ccattttgct ttaatacata ttccaatgcc
    10141 attaagggca ttccgccttt tctgccgcca agaatcgtat gaccttttaa tttgtcaatg
    10201 gtaaagttat cgtctttttc ccttccgaca ataaatgagc catcacgttg agtaagctgt
    10261 gcaaagttaa ctgccaagtc atctcttccc tgtaggtaga catagacgct tgcttcaggt
    10321 cccataaaag ctatatcggc ttcgccgctt accaaggccg ccatacattt gtcggcacct
    10381 ccgccgttag tcaattcaat acttagacct tgcttttcaa ataatcccat ttcaagtgca
    10441 acatactgag gtgtataaaa aatcgagtga gttacttcta taagccgtac tttttgatat
    10501 tccgtgggtt tttcattgca tcctgtaagt actaatgatg taatgagcag cataacaaca
    10561 agcagagaaa taaacttttt catttcatcc tcccaaaatt agtctttcga atataatatt
    10621 caatttatct ctaaagcgtt acataactct ttataaaagt agtggaaaag taatggattc
    10681 tatggtatat tatctttgta tgcaatatat atataataaa aacatgagta gcagacggag
    10741 aataatatgg ctatatcttt ttcggattat ttaatgcaga ttactgataa ccccatgttg
    10801 cttattactt cgattttaac catggcggtt atcttggtca atggttggac tgatgcacca
    10861 aacgctattg ccacatgcgt ttctacccgt tccatatcag cgaaatcagc gattgtaatg
    10921 gctgcaattt ttaatttttt gggtgtttta ttgatgacag taattaatgc aaatgtggca
    10981 caaacaattt ataacatggt tgactttgga ggtaattcat atgatgcact ggttgcttta
    11041 tgtgcagcat tatttgcaat cgtaatatgg gctaccgctg catggtggtt tggaattcca
    11101 acaagtgaaa gccatgcact gatagcgggt atatcaggtg ctgccattgc cttgcataac
    11161 gggttacaag gaataaacgg cagtgaatgg attaaggtat tgtatggatt aggtttatct
    11221 acctttttag gctttaccct aggttggatt gttgtaagga tggtagagat tatttttagg
    11281 tcaatgagca ggatgaaagc caatatcctt tttacaaatg ctcaagttgc aagcggagca
    11341 gctatggcat ttatgcacgg tgctcaagac ggacaaaagt ttatgggagt atttatgctt
    11401 ggtatatatt tagcaggtgg acaagcaaat gtaaccactt ttcaaatccc tttatggatg
    11461 atgatattat gctcagctat tatgggcatc ggaacatcaa taggtggtta taggataatc
    11521 aaatcagttg gaatggatat gtgcaggttg caaaaatatc aaggtttttc tgcggatatg
    11581 gctgctgcca cttgtttgtt tatttcttca atgaccggaa taccggtaag cactactcat
    11641 actaagacta ctgcaataat gggtgtggga gcagcaagaa gattttcttc agtaaattgg
    11701 ggaattgtcg gtgaaatggt tatgacatgg attttgacat tcccgggatg tggaataatc
    11761 ggatatttaa tggcgaaatt atttataaat atattttaat gaggtgaaat atggcaagga
    11821 aaacagatta ctttgaaggc tttataaaat tagccgaata cagctataat gctgcaaagc
    11881 tcttagacga tacattgagg gactttaata aagattcttt acagaaaacg atgaaattaa
    11941 tgcatgaaat agagcataca gccgatttgg aaggtcatga aataactaaa aagcttttaa
    12001 aggaatttat tacccctatt gaacgtgaag atattatgct tttgatacac aaaattgacg
    12061 atattacaga ttgtattgaa gatgtgctgc ttcatatgta tatgtataac gtaagggtta
    12121 tcaaagagga tgcaataaaa ttttccggat tgattttaag cagttgtgaa gaattaatcg
    12181 gagcattaaa agaatttaaa aatttcaaaa aatcgaagga aatacgaagc agaattattg
    12241 ctgtgaataa gattgaggaa gaaggagata atctttatac agaaattgta agaaagctgc
    12301 atcttacatc aatgaatgcc atagatatta tgacatggac aatagcgtac aatcgacttg
    12361 aaaaatgttg tgatgcttgt gaagaagctg ctaatgtcat agaaagtatt attttgaaga
    12421 atttataagc gaaaagataa agattgttaa aaaattctct atatttgtta aaaaatgtgt
    12481 tgcaatataa atgcaagatt gataaaatat atccatagga ggcagcatat atggataaac
    12541 atcaaaaaat ttcatctgca gtaataagaa ggttacctaa atattatagg tacttagggg
    12601 aattaaacaa agttggaatt accaaaactt cttcaagaga actaagtgaa atgacaggtt
    12661 tctctgcttc acaaataaga caagatttaa ataactttgg gggattcgga cagcagggat
    12721 ttggctacga tgtgggaaat ctgcgtaatg aaattggaaa aatactagga ttagacaaaa
    12781 agtataaaat tataattgta ggtgcaggaa acataggcca ggcaattgct aattatacag
    12841 gtttttatga agctgactat gaagttgttg cgttgtttga taaaaatccg aagcttgtag
    12901 gattatctat cagaaatgca ttgataatgg attcagataa tttagaagaa tttcttaaaa
    12961 aagagcatat agacattgca gttatctgca ctccaaagag tgtaagccaa caagttgccg
    13021 agcaattggt gcgttgcggc ataagggcta tttggaactt tgctccaaag gatttaaaaa
    13081 tgcctgaaga agtatatgtt gaaaacgttc atttaaatga aagtttattt tctctaactt
    13141 attattacaa caagatgaaa aaagaaagcg gaaaataatg cataataagc agaagtctag
    13201 gcttctgctt ttgcattcaa tggttttttg tatgaaaaaa atttatatta ataaattttc
    13261 ttgttagtgc aaataatatt ttttatcctt gaaataattt gaaatgggtg atagaataag
    13321 attgtccgat aatattgtta ttaatttagc aatattaaag ataaaaatac ttataaacta
    13381 ataaatttgg aggtatagta atggcaaaag aggttttaaa tccattggaa aactctcaaa
    13441 tgcaagtaaa aaaagcttgt gacgctttag gattgcaccc ggatgtgtat gagattttaa
    13501 aggaaccaca aagagttatt gaagtcaaca ttcctgttag aatggatgat ggaagcatga
    13561 aagtattcaa aggcttcaga gcatcgcata atgatgctgt tggtcctgca aaaggcggag
    13621 ttagattcca tcccggcgta acattggaag aagttaaagc tctttctatc tggatgacgt
    13681 ttaaaggcgg agtaatgggt cttccttatg gcgggggtaa aggtggagtt atatgtgatc
    13741 cattgacatt atctcaagga gaattagaaa gaatttctag aggctatatc caaaaactgt
    13801 ataagtacat aggagaaaaa gaagatatac cggcacctga cgcaggaaca aacggacaaa
    13861 ttatggcttg gatggtggat gaatatatca aattaacagg cggctctgca tcattaggtg
    13921 ttataaccgg aaaacccgta gcatggggcg gctcagaagg cagaaatgaa gcaacaggtt
    13981 ttggtattgc agtagttacc agagaagcag caaaaaaata tggaattgat cttacaaaag
    14041 caaaagttgc cgtacaggga tttggtaacg taggtcgtta cacagttaaa aatattcaga
    14101 gacaaggcgg tacagtagta gctgtaggag aatgggcacc aaaagttgg
//
LOCUS       DAOPUE010000047        13704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4570_length_13704_cov_10.045571, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000047 DAOPUE010000000
VERSION     DAOPUE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13704
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_13704_cov_10.045571"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..957)
                     /locus_tag="PKH42_05695"
     CDS             complement(13..957)
                     /locus_tag="PKH42_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOA19958.1"
                     /translation="MKHIFIINPIAGNGKLQEKLEKTIHLELKDKEIDYEVYITKSKE
                     DTRNFAENKCKENIQAVLYSCGGDGTLHEIINGACRYKHVSIAIIPIGSGNDFIKNFK
                     NSANFSDINLQTEGESIELDLIKVNNEYSANVLNIGLDADAAYNMDKFRKFPFIQGTT
                     RYYLSMLYSLFGKLGKHIEVNTENGVINGTFLIGVIANGQYYGGGYKCAPKAKVNDGL
                     LDLCFVEKISRAKILNIIGSYKKGEHLDNPKINKYIIYDKINQANIKFDKQTNVCMDG
                     EIYTYNELNISTEKNALKFWIPKGVQLQTQQIENHSFV"
     assembly_gap    1189..1288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1344..1973)
                     /gene="upp"
                     /locus_tag="PKH42_05700"
     CDS             complement(1344..1973)
                     /gene="upp"
                     /locus_tag="PKH42_05700"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOA19959.1"
                     /translation="MNNITVVSHPLIQHKLTMLRDKNTPPKDFRELVREISMLLAYEV
                     TRSLPLKDIDIETPICKTTGKILSGADIAIVPVLRAGLGMVDGMLDLIPNAKIGHIGL
                     YRNEETLEPVEYYCKLPSDIDKRIVIITEPMLSTGGSMNGALNMLKKRGAKKIKSIHL
                     VCAPEGLKRVTETHPDVEIYTAAIDEKYNEIGYIVPGLGDAGDRLFGTK"
     gene            2133..2498
                     /gene="acpS"
                     /locus_tag="PKH42_05705"
     CDS             2133..2498
                     /gene="acpS"
                     /locus_tag="PKH42_05705"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOA19960.1"
                     /translation="MIKGTGIDITEVERIKKNMGNIRFLEKIYSNRELEYLKTRKFNP
                     QTAAGMFAAKEAVAKCLGTGFSNFGPCHIEILKDENDKPYVFLLSNALKRAEELNIKN
                     IYISISHTQEYAVAHCVAE"
     gene            2660..3157
                     /locus_tag="PKH42_05710"
     CDS             2660..3157
                     /locus_tag="PKH42_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19961.1"
                     /translation="MRLIIFILVLLMALSGCTAEDKPFILPVNYSANALMKVYTDKTE
                     NDYKINIICRDGNYSIRTETENEAWNCAYVSGNRCILNNDKFPESSVTIENYKLNDTL
                     LHDFDFGKFDVFEDIPEELVYWDGEYKHVLNFSKDNMLPKTIFIYKYDKLVKAIQYEK
                     INIEE"
     gene            3257..3532
                     /locus_tag="PKH42_05715"
     CDS             3257..3532
                     /locus_tag="PKH42_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOA19962.1"
                     /translation="MAENKKVLISIPANLLRELDNAVKEEGTNRSDFIRKSIRFYLIE
                     KRKLEIRNKMKAGYLEMSKINKSITEEYSEGDYEDFLSYETKLLGSE"
     gene            3534..3884
                     /locus_tag="PKH42_05720"
     CDS             3534..3884
                     /locus_tag="PKH42_05720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOA19963.1"
                     /translation="MIVKRGDVYYADLSPVIGSEQGGVRPVLVVQNDIGNRYSPTVIV
                     SAITSQINKAKLPTHIEISSEDFSLPKDSVVLLEQIRTIDKRRLKEKIGRFDKNLMGE
                     VDDCLKISLGLIDF"
     gene            complement(3966..5276)
                     /locus_tag="PKH42_05725"
     CDS             complement(3966..5276)
                     /locus_tag="PKH42_05725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOA19964.1"
                     /translation="MNYDEALQFIHSTYKFGSKLGLERIKRLMELLGNPQDCCKIIHV
                     AGTNGKGSTCNLIHDVLMESGYKTGLFISPYLEEFTERIQINKQKIDKDSLTRIAELV
                     RDKVNTMVSEGYDHPTEFEVVTAIGFKYFQEQNIDFLVLEVGMGGRFDATNVIKKPLL
                     SVITSISYDHMEQLGNTLEKIAFEKAGIIKENSTVIIYPQTINITNVIKDVAKEKNAK
                     VYEADKSNIEKTKSDITGQWFKYLKRDVFELPELKINLLGEHQLLNALTALSALEIIK
                     DKGYDISTKSIENGFSNCKHPGRFEIINKNPYVILDGGHNIDGIRSFTNTLKEYFKDK
                     VTFFFGILKDKSPEEALQLILPLAKEIYTLTPLSPRALKAADLAGLIKKYSDIKVIPL
                     ENYEDMLPIIKNAGKNEIIAFSGSLYMIGNVRTLLRNNGITNNL"
     gene            5645..7504
                     /locus_tag="PKH42_05730"
     CDS             5645..7504
                     /locus_tag="PKH42_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOA19965.1"
                     /translation="MIDVKKLDRVLLSVEKPARYVGSELNSVVKNKDIDIRFAFAFPD
                     IYEVGMSHTGLHILYHLMNSMENVWCERVFAPWTDMEEVMRKNNIPLYGLESKDDLTA
                     FDFVGFTLQYEMSYTNVLNMLDLGNIPIKSKDRGNDMPLVIAGGPCVYNPEPLHEIID
                     LFVVGEAEEVLPELLNLYNEEKKNVYDKLKFLEKAASIEGIYIPQFYEEIYNNDGTIK
                     ERIVLNPNAKETIKKRIIKDLSKSYYPEKSILPYIDVVHDRVVLELFRGCTRGCRFCQ
                     AGMVYRPVRERSTDTLINQAERLLSSTGHDEVSLTSLSSGDYSCLPDLTIRLLDKYEN
                     QNISISLPSLRLDSMTFDIIERLQEVRKSGLTFAPEAGTQRMRDVINKNLTEEQILGP
                     VETAFKLGWSTVKLYFMIGLPGETMEDVLGIKELSYKIKDKFFHRPKDEIKGNLKINV
                     SAACFVPKPFTPFQWAAQNNMDEFYEKAKVLQREIKDKKISFSYHEPKLSYLEAVFAR
                     GDRRLSKALIKAWEKGCRFDGWYDLFDFDKWMSAFEETNIDPDFFALRQREVREILPW
                     DFIDIGVKKEYLVSEYEKALDFDTTPDCRQRCTNCGVNYNLWGGACPHVQNNM"
     gene            7485..>7807
                     /locus_tag="PKH42_05735"
     CDS             7485..>7807
                     /locus_tag="PKH42_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOA19966.1"
                     /translation="MCKIICKYSKTGYLKYISHLDVLRFIQRSVKRAGIKAKYSEGFN
                     PHMKTSFGYPLSLGIESIGEYFELELNENIEPELFKDKMNSVMPKEMQIIKADYSNDA
                     ESLMK"
     assembly_gap    7808..7907
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7908..9341
                     /locus_tag="PKH42_05740"
     CDS             <7908..9341
                     /locus_tag="PKH42_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOA19967.1"
                     /translation="KLIDYLFELNNNQKVVGNIYKGKVINILPGMQAAFVDIGLNKNA
                     YLFLDDLLSDKFLKELKIKKKDVSNISKVLKTGDELLVQVVREPMGEKNISVTTNISL
                     TGKFIAFIPNNNVVNLSKKIKDPDERKRLEEIGKSIILNGNGMIIRTFAKGSHKEAIE
                     KEYKMLSNIYMKIEKEYKYSYAPKLLHKSNSLIDRLFLDYIDSSVEEIYVEDKITKDK
                     FNDMISGLPGDILKNIKIIQAQRVFENFNIASQINMLFERRVELDNGGFIIIDVTEAL
                     TVIDVNSGKYTGSRNMEETALAINLSAIEEIARQIKMRNLSGIILIDFIDVKKEESIN
                     LLIAKAKSLFRDDKTKTNVIGMTKLCIMEITRKRNTENFFNLITEECSHCSGSGRSIS
                     KMYIFIKIEGIIKNIKHNTSSEAVILNVGCILYNKIIKSCMDIIAEIEKKYDIKIFIE
                     QDDKILTDEIIIGKIGKLSNVNGIYQI"
     gene            9573..9884
                     /gene="rplU"
                     /locus_tag="PKH42_05745"
     CDS             9573..9884
                     /gene="rplU"
                     /locus_tag="PKH42_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOA19968.1"
                     /translation="MYAIIETGGKQYRVQEGDVLRVEKLDIAEGETVKFDKVLLVAEE
                     GKVNVGKPYVDGAVVEAVVENHGKAKKIIIFKYKSKKDYRKKQGHRQPYTQIKVEKIV
                     G"
     gene            9886..10212
                     /locus_tag="PKH42_05750"
     CDS             9886..10212
                     /locus_tag="PKH42_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOA19969.1"
                     /translation="MITVTIYRTKDEFKGFIVEGHSDYAEEGLDIVCASVSILSYTAL
                     NSLNLVAGITPENIEYTVDDEGVMSLRTLENNNKTDIVYRTFMVGIELLLEDYSDYIT
                     LKFEEV"
     gene            10215..10508
                     /gene="rpmA"
                     /locus_tag="PKH42_05755"
     CDS             10215..10508
                     /gene="rpmA"
                     /locus_tag="PKH42_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOA19970.1"
                     /translation="MLLKLNLQLFAHKKGVGSSRNGRDSNSKRLGVKRTDGQFVLAGN
                     ILVRQRGTKLHPGVNVGRGSDDTLFAMIDGKVKYERKDKTRKQVSVYPVELDA"
     gene            10600..11874
                     /gene="obgE"
                     /locus_tag="PKH42_05760"
     CDS             10600..11874
                     /gene="obgE"
                     /locus_tag="PKH42_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOA19971.1"
                     /translation="MFVDIAKISVKAGKGGNGSVAFRREKYIPMGGPAGGDGGDGGSV
                     ILVADEGLRTLMDFRYKKHYHAENGEDGRGKKQYGSAGEDLYLRVPVGTLVKDAETGI
                     VLADLKTHGQQYVAARGGRGGKGNVKFKSSIRRTPRFAQPGTKGDEREIILELKLLAD
                     VGLIGFPNVGKSTILAAITSAKPKIANYHFTTLKPNLGVVSIGEGHSYVLADIPGLIE
                     GASEGAGLGLDFLRHIERTKLLLHVIDASGQEGRDPVEDFHKINDELKQYSNKLSGKE
                     QIIVLNKMELPDAINNAERIKKEFSEKYEIIQVSAATGSGLDFLKKRAYERLLEIEEE
                     IEFVDEEAVESFLDKKERDTILVTKEDGYYSAEGDFLERLVASTNFDDFESFSNFQKV
                     LIDKGVIQKLKELGAEEGDLISVCGIEFDFVD"
     gene            11886..12176
                     /gene="yhbY"
                     /locus_tag="PKH42_05765"
     CDS             11886..12176
                     /gene="yhbY"
                     /locus_tag="PKH42_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOA19972.1"
                     /translation="MLTGKQRSYLKALANSVDTIMQIGKGGITDSVLKQIDDALNARE
                     LIKVSVLKNSMLDAKSVANEVAESLGAEYVQSIGGKFVLYRESKEKKINIPK"
     gene            12515..13279
                     /locus_tag="PKH42_05770"
     CDS             12515..13279
                     /locus_tag="PKH42_05770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOA19973.1"
                     /translation="MIKGILFDKDGTLIDFSLWRNAGINTIQTILNEYNLNNNKLNIE
                     LQRAIGIKEKGVEPFGALAYKSHEDVAYELHCILNKYINIDFDKFETHVVGLLRNEVL
                     RDDVEFKEIVSIRKLYEHLNSNNIKMGLATADSMQSAMHMINKLNLNDSFDFIGSYDG
                     KMKRKPHKDMCMKFCSMYNLEPGEVAIVGDSYSDMLFALNSGAIGVGVLSGVSSKINL
                     KDVANVIVPSVESLFDRNVLEALDEKSYEARELRTA"
     gene            13381..13575
                     /locus_tag="PKH42_05775"
     CDS             13381..13575
                     /locus_tag="PKH42_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19974.1"
                     /translation="MNELEKIKKMYDNGFRCIRYDDTKDGDMCIYFKNFDNEESEALR
                     VSDFEQKMQIKSFIKENSMK"
BASE COUNT         4635 a         2015 c         2688 g         4166 t
ORIGIN      
        1 ctatccccaa atttacacaa atgaatgatt ttcgatttgt tgtgtttgca attgaacgcc
       61 ttttggaatc caaaatttca aggcgttttt ttccgtcgat atatttaatt cattgtaagt
      121 atatatttct ccatccatgc agacgttggt ttgtttatca aattttatat tagcttgatt
      181 aatcttgtca tagataatat atttattgat tttaggatta tctaaatgct cacctttttt
      241 gtaacttcct attatattta atattttagc tcttgagatt ttttccacaa agcataggtc
      301 aagaagaccg tcatttactt ttgcttttgg agcacacttg tatcctccgc cgtagtattg
      361 tccgtttgct atgacaccaa tcagaaatgt tccgttgata acgccatttt ccgtattaac
      421 ttcaatatgc tttcccaatt taccaaacaa agaatacagc atggaaagat agtatctggt
      481 agtgccttga atgaaaggaa acttcctgaa tttatccatg ttgtaagctg catcggcatc
      541 aagaccaata tttaatacat tggcagaata ttcattgtta accttaatta aatccagttc
      601 tatgctttca ccttccgtct gcaggttaat atcgctgaaa ttagccgaat tcttgaaatt
      661 cttaataaag tcattgccgg agcctatcgg tattatggca atactaacat gtttatacct
      721 gcatgcgcca tttattattt catgaagggt cccgtcacct ccgcatgaat acaagacagc
      781 ttgtatattt tctttgcatt tattttctgc aaagtttctt gtgtcttctt tagatttggt
      841 gatatatacc tcataatcaa tttctttatc ttttaattca aggtgtatag ttttttctaa
      901 cttttcttgt aatttgccgt tgcctgcaat aggatttatt ataaatatgt gtttcatgtc
      961 ctctccatct gtttgttatt gctatgaata acatttttgt cattctgagg gcttcagccc
     1021 gaagaatctc atgagatcct tcgcttacgc tcaggatgac aaaagggtgt tgtctgcaga
     1081 tccttcgttt cactcaggat gacaaaaggg tgttgtctgc agatccttcg tttcactcag
     1141 gatgacaaaa gggtgttgtc tgcagatcct tcgtttcact caggatgann nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt cgtttcactc aggatgagcg accaacacca
     1321 aattaaaaat ttgggttgtc tgctcacttt gtgccgaata gtctgtctcc tgcatctccc
     1381 aaaccgggta cgatatatcc tatttcatta tatttttcat ctatggcagc agtgtagatt
     1441 tcaacatccg ggtgagtttc cgtaactctt ttcagtcctt cgggtgcgca taccaaatga
     1501 attgatttga ttttctttgc tcctcttttc tttagcatat tcaatgcacc gttcattgaa
     1561 cctccggttg acagcatggg ctctgtaata attactattc ttttatcaat atctgatgga
     1621 agcttgcaat aatattcaac gggctcaaga gtttcttcat ttctgtacaa accgatgtgg
     1681 ccgattttag catttgggat taaatccaac ataccgtcca ccatgccgag gcctgctctc
     1741 agaacaggta caattgcaat gtctgctcca gatagaattt tacctgtagt cttgcatatg
     1801 ggtgtttcta tgtcaatatc tttcagtggc aggcttcgag ttacttcgta tgccaagagc
     1861 atggaaattt ctcttactaa ttctctaaaa tctttcgggg gtgtgttttt gtctctcagc
     1921 attgtcagct tgtgctgaat cagcggatgg gatactacgg taatattatt catatgatac
     1981 tccttattgt taaatgtaat ttaagaattg tattgtaact aaacatgtaa tataattatt
     2041 atatacaaaa agccctttgt caattaaaaa tttttaaagg atgagggaca caccttcata
     2101 atcaggtaaa ggaatgtccc tgtggaggaa gtatgattaa aggcactgga atagacatta
     2161 cggaagttga aagaataaaa aagaatatgg gaaatatccg gttccttgaa aagatatatt
     2221 caaacagaga attagaatat ttaaaaacaa ggaaattcaa tcctcaaaca gctgccggaa
     2281 tgtttgctgc aaaagaagct gttgccaaat gtttgggaac gggattttca aatttcggtc
     2341 cttgtcacat tgaaatatta aaggatgaaa atgataaacc atatgttttt ttattaagca
     2401 atgcattaaa aagagcagaa gagttaaata taaaaaacat atatatttca atatctcaca
     2461 cccaagagta tgccgtagca cattgcgtag ccgaatgatt tatggcatat atttcaaaat
     2521 ttcatcataa aatgaagtag gtatggggac acaccttcat agaaggatag actgtgaaga
     2581 aagtccatga aggcatatcc ccatacttaa aggatatatc tcaaagtccc attcgattat
     2641 gatggaagca ggttttggta tgagattaat aatatttatc ttagttttac tgatggctct
     2701 aagcggttgt actgcggagg ataaaccgtt tattctgcca gttaattatt cagctaatgc
     2761 attgatgaag gtatatacgg ataaaacgga aaatgattat aaaatcaata ttatttgcag
     2821 ggacggcaat tacagcatca ggacggaaac tgaaaatgaa gcatggaact gtgcatatgt
     2881 ttcagggaac agatgtatac taaacaatga taaatttcct gaaagcagcg ttacaatcga
     2941 aaattataaa ttaaatgata cattgctgca tgattttgat ttcgggaaat ttgatgtatt
     3001 tgaagacatt ccggaagaac tggtttattg ggatggtgaa tacaagcatg tattaaattt
     3061 tagcaaagac aatatgcttc caaagacaat ttttatttat aaatatgata aattggtaaa
     3121 agctatacag tatgaaaaaa taaatattga ggaataatat taataattat ttacgaataa
     3181 tacaaatgtt accatacaac ttatatgttt attgtgttaa aatataatat aactgtgcaa
     3241 ttgcgaggtg tgtatcttgg cggagaataa aaaagtactg atttccatcc ctgcaaattt
     3301 gttgagggaa ttggataatg cggtaaaaga agaaggaaca aacagaagtg actttatcag
     3361 gaaatccata aggttttatt tgattgaaaa aagaaagctt gaaatcagaa acaagatgaa
     3421 agcaggctat ttggaaatga gtaaaatcaa caagagcatt actgaagaat actcagaagg
     3481 ggattatgaa gactttttaa gctatgaaac taaattatta ggaagtgaat agtttgattg
     3541 ttaaaagggg agatgtatac tatgccgatt tgagccccgt aattggctcg gaacagggag
     3601 gggtaagacc ggtattagtt gtgcaaaatg atattgggaa tagatacagc cctacagtga
     3661 ttgtatctgc tattacgtct cagataaaca aggcgaagct gcctactcat attgaaataa
     3721 gttctgagga tttcagcctt cctaaggact ctgttgtttt gctcgaacaa atcagaacaa
     3781 tagataaaag aaggcttaaa gaaaaaattg gacgttttga taaaaacctc atgggagagg
     3841 tggatgattg tttaaaaatc agcttggggc tgatagactt ttagaagacc taaccctgtt
     3901 tttcagaatc ttgtctgaaa aacagggtta ggtttttctt ggcgaatttc cagtttatgc
     3961 gacttttaca ggttatttgt tataccatta tttcttaaca gtgtccttac gtttcctatc
     4021 atatataatg agcctgaaaa ggctattatt tcatttttgc ctgcgttttt aataataggc
     4081 agcatatctt cgtagttttc caaaggaatc acttttatat ccgaatactt ttttattagt
     4141 ccggctaagt ctgctgcttt taatgctcgc gggcttaaag gagttaaagt ataaatttct
     4201 tttgctaatg gcaatattaa ttgcaaagct tcttcgggag atttatcttt taaaataccg
     4261 aagaaaaatg ttactttgtc cttgaaatat tcctttaaag tgtttgtaaa gctgcggatg
     4321 ccgtcaatat tgtgcccccc gtcaagaata acataggggt ttttatttat tatttcaaat
     4381 ctgccgggat gtttacagtt tgaaaaacca ttttctatac tttttgtgct tatgtcgtaa
     4441 cctttatctt taataatttc caaagccgaa agtgcagtca gtgcgtttaa aagctgatgt
     4501 tctcccaata agtttatttt tagctccggt agctcaaata catctctttt taaatattta
     4561 aaccattgtc cagtaatatc ggattttgtt ttttcgatat tgcttttatc agcttcatag
     4621 acttttgcat ttttttcttt tgccacatcc tttataacat ttgttatatt tattgtctga
     4681 ggatagatta tgacagtgga attttccttt attatgcctg ctttttcaaa ggctatcttt
     4741 tccaatgtgt ttcccagctg ctccatgtgg tcatagctta tggaagtaat aacgctgagc
     4801 aagggttttt ttattacatt ggttgcatca aatcttcctc ccattccgac ttcaagaacc
     4861 aaaaaatcaa tattctgctc ctgaaagtat ttaaagccta tggcggttac aacctcaaat
     4921 tcggtaggat gatcatatcc ttccgaaacc attgtattaa ctttatctct taccaattcc
     4981 gcaattcttg tcagggaatc tttatcaatt ttctgcttat taatttgaat tctttccgtg
     5041 aactcttcta agtagggact aatgaataaa cctgtcttgt atcctgattc catcagcaca
     5101 tcatgaatta agttgcaggt tgatccttta ccgtttgttc ctgcaacatg tatgattttg
     5161 cagcagtcct gcgggttacc caataattcc ataagtcttt tgattctttc aagaccgagt
     5221 tttgagccga atttatatgt agaatgaata aattgcaatg cttcgtcgta attcataata
     5281 acctctctaa tataatttta ttaattttac aatacttttt tttctttttc aatgtaattg
     5341 tttaatacag tattgctttt ctataaaaat aataaaaata tacgctcacg gtttcggcca
     5401 cgaataattc tactagctca ttccggctaa aaatcctaca gcttgcaaac tcgcttcgct
     5461 caaacagtgc aagctgttta acggattttt aaccttcatt cgctaagaat tatactcgtg
     5521 tcctgcaaat gttcgcttaa tatttttatt attttttaat taagggagta taataataaa
     5581 agttgtcaac aaatgtttat tatgttaaaa tatcatagaa tttaaggaag tcgaagggat
     5641 taatatgata gatgtaaaaa aactcgacag agtgcttctt agtgttgaaa aacctgctcg
     5701 ttacgttgga agcgagctta actcggtggt taaaaataaa gatattgata taagatttgc
     5761 atttgcgttt ccggatatat atgaagtcgg aatgtctcat accggactgc atattttgta
     5821 tcatttaatg aacagtatgg aaaatgtatg gtgtgaaagg gtatttgcac cttggacaga
     5881 tatggaagaa gttatgagga aaaataacat tcctttgtat ggcttggaaa gcaaggatga
     5941 ccttactgcc tttgattttg ttggatttac tctccaatat gaaatgagct acacaaatgt
     6001 tttaaacatg cttgatttag gtaatatacc tataaaatca aaggacagag gtaatgatat
     6061 gcccttggta attgcaggag gtccttgtgt atataatccg gagcctctgc atgaaataat
     6121 agatttattc gttgttggtg aagctgaaga ggttttgcct gaactgttga atttatataa
     6181 cgaagaaaag aaaaatgtct atgataaact taaatttctt gaaaaggcgg catcaataga
     6241 aggtatatat attccgcaat tttatgaaga aatatataat aacgacggta cgattaaaga
     6301 aagaatagtg ttaaatccaa atgccaaaga aacaataaag aaacgtatta ttaaggattt
     6361 aagcaaaagc tattatcctg aaaaaagtat actgccctat attgatgtgg ttcatgacag
     6421 agtagttttg gaattattcc gtggctgtac gagaggctgc cgtttttgtc aggccgggat
     6481 ggtttacaga cctgtaaggg aaagaagtac cgatacgctg ataaatcaag ccgagagact
     6541 tctaagctca acaggtcatg atgaagtatc attaacttcc ctaagctccg gagattattc
     6601 atgtctaccg gatttaacta taaggctgtt agataagtac gaaaatcaaa atataagtat
     6661 ttctctgcct tcgttgaggc ttgattctat gacctttgat attatcgaaa gactgcagga
     6721 agttcgtaaa tccggcctta catttgcacc ggaagcagga actcaaagga tgagagatgt
     6781 tataaataag aatctgacgg aagaacaaat tttgggaccg gttgaaactg cttttaaatt
     6841 agggtggtca acggttaaat tgtatttcat gatagggctc cccggtgaaa caatggaaga
     6901 tgtgctcgga atcaaagagc tttcttataa aatcaaggat aaatttttcc acagacctaa
     6961 ggatgagata aaaggaaatt taaaaataaa tgtaagtgcc gcctgctttg taccaaaacc
     7021 ttttacaccc tttcagtggg ctgcacaaaa caatatggat gaattctatg aaaaggcaaa
     7081 agtattgcaa agagaaatta aagataaaaa aatttccttc agctaccatg aacctaaatt
     7141 aagttatttg gaagctgtat ttgcaagggg agacagaaga ttatccaaag ctttgattaa
     7201 agcatgggaa aaaggatgcc gctttgacgg gtggtacgat ttatttgatt ttgacaagtg
     7261 gatgagtgca tttgaagaaa caaacattga ccctgatttt tttgcattga gacaaaggga
     7321 agtaagagaa attttaccat gggattttat agacattgga gttaagaaag aatacttagt
     7381 cagcgaatat gaaaaagcat tagactttga tactactcct gactgcaggc aaaggtgtac
     7441 aaactgcggt gtaaactata atttatgggg aggtgcttgc cctcatgtgc aaaataatat
     7501 gtaaatacag caagacagga tatttaaaat atatttccca cttggatgtt ttaagattta
     7561 tccaaaggtc ggtaaaaagg gcaggcataa aggctaaata ttcggaagga tttaatcctc
     7621 atatgaaaac atcgtttgga tatcccttgt cgctgggaat agaaagtatt ggggagtatt
     7681 ttgaattaga gcttaatgaa aatattgagc cggagttatt taaagacaaa atgaactctg
     7741 tgatgccaaa ggaaatgcag ataattaaag ccgattattc caatgatgcc gaatctctta
     7801 tgaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag cttattgatt
     7921 atctttttga attaaacaat aatcaaaaag ttgtaggcaa tatctataaa ggaaaggtta
     7981 ttaatattct tcccggcatg caagctgcct ttgttgacat aggccttaat aaaaatgcat
     8041 atttgttttt ggatgacctg ctttccgata agtttttaaa agaactgaaa attaagaaaa
     8101 aagacgtttc aaatatcagc aaagtgttaa aaacaggtga tgaactcttg gttcaggttg
     8161 tgagagagcc aatgggggag aaaaatattt ctgttacaac caatatttca ttgacaggaa
     8221 aatttatagc atttattccc aataataatg tagttaatct ttcaaagaaa atcaaagacc
     8281 cggatgagag aaaaagactt gaagaaatag gcaaaagtat aatcttaaac ggaaacggaa
     8341 tgataataag aacattcgcc aagggcagtc acaaagaagc aatagaaaag gaatataaga
     8401 tgctttccaa tatatatatg aagattgaaa aggaatataa atattcatat gcaccgaagc
     8461 ttttgcataa aagcaattct ctcattgaca ggttattttt ggactatata gactcaagtg
     8521 tagaagaaat ctatgttgaa gataaaatta caaaagataa atttaatgat atgatttcgg
     8581 gcttgcccgg agatattctt aaaaatatta aaattataca ggcacaaagg gtttttgaaa
     8641 atttcaatat tgcaagccag ataaatatgc tatttgaaag aagagttgag cttgataacg
     8701 gaggcttcat aataattgat gttacggaag cattgacggt aatcgatgta aacagcggga
     8761 aatataccgg aagccgaaat atggaagaaa cagctttggc tataaaccta tctgcaatcg
     8821 aagaaattgc aaggcagata aagatgagaa atttaagcgg aattatttta attgatttca
     8881 ttgatgttaa gaaggaagaa agtattaatc tgctaatagc taaagccaag tcattgttta
     8941 gggatgataa aacaaagaca aatgttatcg gaatgacaaa gctatgtatt atggaaataa
     9001 caaggaaaag aaacacggaa aactttttta atctgataac cgaagagtgc tcacactgca
     9061 gcggaagcgg cagaagcatt tcaaaaatgt atatatttat taaaattgaa ggaataataa
     9121 agaatattaa gcataataca tcttccgaag ctgttatttt aaatgtggga tgtatattgt
     9181 acaataaaat aatcaaaagc tgtatggata ttattgctga aattgaaaaa aaatacgata
     9241 ttaaaatatt tattgagcag gatgataaaa ttcttaccga tgaaattatc atcgggaaaa
     9301 taggaaagtt gtccaatgta aatggaatat atcaaatata gcagtttcgc aggctccatc
     9361 accattttgc tcactccctg tggtcgtgca aaatgggaat gtgctgcgtc tgacctacca
     9421 tccatttttc agaaaaatgg ggttaggtca tcaaaaaata agttgacaat ttttttatag
     9481 tttgatacaa tcaatatctg ttagagccgc acggatcagg ttgaaaacgc taagtacctg
     9541 caatggcgag actgggaaga ggaggtaaaa atatgtacgc aattattgaa acaggtggaa
     9601 aacaatatag agttcaagaa ggcgatgtgt taagagttga aaaacttgat atcgctgaag
     9661 gtgaaaccgt aaaatttgat aaggtattgc ttgtagccga agaaggaaaa gtgaatgtag
     9721 gcaaaccata tgttgacggt gcagtagttg aagcagtagt tgaaaaccac ggcaaagcta
     9781 aaaaaataat aattttcaaa tacaagtcta aaaaagacta caggaaaaag caaggtcatc
     9841 gtcagccata tactcaaatt aaggttgaga agatagtagg atagtatgat tacagttacc
     9901 atatatagaa ccaaggatga attcaaaggt tttatagtag aaggccattc ggattacgct
     9961 gaagaaggat tggacatagt atgcgcatca gtttccattt tgtcttacac agcattgaat
    10021 tcattgaacc ttgtggcggg aattacccct gaaaacattg aatataccgt cgatgatgag
    10081 ggagtaatga gtttgaggac attagagaat aataacaaaa ctgatatagt gtacagaacc
    10141 ttcatggttg gaattgaatt attattggaa gattacagcg attatattac acttaaattc
    10201 gaggaggtgt aaaaatgtta ttgaaattaa acttacagtt atttgcacat aaaaaaggtg
    10261 ttggtagctc aagaaacggc agggacagca attctaagag attgggagta aaaagaaccg
    10321 acggtcaatt cgtgttggcc gggaatattc ttgttagaca aagaggtact aaacttcatc
    10381 cgggagttaa cgtaggcaga ggaagtgatg atacgctatt tgccatgatt gacggaaagg
    10441 ttaagtacga aagaaaagat aaaacaagaa aacaggtcag tgtatatcct gttgaattag
    10501 atgcataata aaagaaaaga cctaccatac ggtaggtcat ttttagatga agaaatataa
    10561 agattgtcag gatgacaaga gtggcggaaa ggaacaaaaa tgtttgtaga tatagcaaaa
    10621 ataagtgtaa aagcaggtaa gggcggaaac ggaagcgtag cattcagacg tgaaaaatac
    10681 ataccaatgg gcggacctgc aggcggcgac gggggcgacg gcggaagcgt cattttggtt
    10741 gccgatgaag ggcttagaac attgatggat ttcagatata aaaaacatta tcatgcggaa
    10801 aacggtgaag acggaagagg gaaaaaacaa tatggttctg ccggggagga cctttatctg
    10861 agagttcctg tcggaacttt ggtaaaagat gcagaaacag gcatagtact ggcagactta
    10921 aaaacccacg gacagcaata tgtagcagca agaggcggac gcggaggaaa aggaaatgtt
    10981 aaatttaaaa gttccataag gcgtactccc cgttttgcac agcctggaac caagggcgat
    11041 gaaagagaaa ttattcttga gcttaagctt cttgccgatg tcggattaat agggtttcca
    11101 aacgtgggga aatcaacaat attagcagct ataacaagcg ctaaaccgaa aatagcaaat
    11161 tatcatttca cgactttaaa accgaatctt ggagttgtga gcataggaga aggacacagc
    11221 tatgtgcttg cagatattcc ggggcttatt gaaggtgctt ctgaaggtgc aggtctcggg
    11281 cttgattttt taaggcatat tgaaagaaca aagctgcttt tgcatgttat cgatgcttcc
    11341 ggtcaagagg gaagagaccc ggttgaagat ttccataaaa taaatgatga gcttaaacaa
    11401 tacagcaata aactatccgg aaaagaacaa ataattgttt taaacaaaat ggagctgccg
    11461 gatgctataa ataatgccga aagaattaaa aaagaatttt cagaaaaata tgaaataatt
    11521 caagtttctg ccgcaacagg cagcggactt gactttctta agaaaagagc ctatgaaaga
    11581 cttttagaga ttgaagaaga aattgaattt gttgatgaag aagctgttga aagtttctta
    11641 gacaaaaaag aaagagatac aattcttgta acaaaagaag acggttatta ttcagcagaa
    11701 ggagattttc ttgaaagact tgttgcatca acaaattttg atgattttga atctttttcg
    11761 aattttcaaa aggtactgat tgacaagggt gtaatacaga aattaaaaga actcggcgct
    11821 gaagaaggag acttaatcag cgtatgcgga atagaatttg attttgtaga ttgaaaggag
    11881 agtaaatgct taccggaaaa caaagaagtt atttaaaagc tttggcaaat tctgttgata
    11941 caataatgca gataggcaaa ggcggtatta ctgacagcgt attaaaacaa attgacgatg
    12001 cacttaatgc cagagaatta atcaaggtga gtgtattaaa aaacagcatg ctggatgcaa
    12061 agtctgttgc aaatgaagtt gcagaatctc taggtgctga atatgttcaa agtataggcg
    12121 gcaaatttgt tttatacaga gaatctaaag aaaagaaaat taatatacct aagtaaaagt
    12181 caggtgccgg aatgcaatca ttcatgcacc tgattcgtca gtgtcattct gaacgcatat
    12241 gaagaatctt atgagatctt tcgctcggga aaacaaagcg cattcattta tcgtggtgta
    12301 gataatacgt gatgattctt atcatatttg tttgaatttc aaatttatat cacatttcag
    12361 ctttttctta atataatagt actccccgca atttaagaat gtttaaagaa tcatattata
    12421 aaataaaaaa gatgcactta tttatacgta ttacaatatg agcatatcat atttattttc
    12481 gtatttacaa tatgaaatat aaggaggtga taaaatgata aaaggaatac tgtttgataa
    12541 ggacggcacc ttgatagatt tttcattgtg gagaaatgcc ggaatcaata caattcaaac
    12601 aatactaaat gaatataatt tgaataataa caaacttaat atagagctgc aaagagctat
    12661 cggcataaag gaaaagggag tggagccttt tggtgccttg gcttataaat cgcatgaaga
    12721 tgtggcttat gagcttcatt gcatactaaa taagtatata aatatagact ttgacaaatt
    12781 tgaaactcat gtggtcggac tattgagaaa tgaagtttta agggatgatg ttgagtttaa
    12841 agaaattgtc agcataagaa aattgtatga acatttgaat tcaaacaata taaaaatggg
    12901 tttggcaacg gcagattcca tgcaatcagc aatgcatatg attaataagc tgaacttaaa
    12961 tgacagcttc gactttatag gctcctatga cggtaaaatg aaaagaaaac ctcacaagga
    13021 tatgtgcatg aaattttgca gcatgtataa tttggagccc ggagaggttg caatagtggg
    13081 ggattcatat agcgacatgc tctttgcatt aaattcaggt gcaatcggag taggggtact
    13141 gtccggagta agcagtaaaa ttaatttaaa agatgtagcc aacgttatag ttccttctgt
    13201 tgaatctttg tttgacagaa atgtgctaga agccttagat gaaaaaagtt atgaagcaag
    13261 ggagcttcga acagcataag cctgtgcaag aaatgcaata aggcttattt ttttctttaa
    13321 ttcagtcata atttccttta tttggtcaat aatatgtata aaacataagg ggtgaatatt
    13381 atgaacgaat tagagaagat taaaaaaatg tacgataatg gattcaggtg cataaggtat
    13441 gatgatacta aggacggcga tatgtgcata tatttcaaaa atttcgataa cgaagaatca
    13501 gaagccttaa gagtttcgga ttttgaacaa aaaatgcaga taaaaagttt tatcaaagaa
    13561 aattccatga aataagaggg aacttaagtt tacagttcct atttatgtcc aattatttct
    13621 tgtaaaacga ttccttctaa tatataatga atgcaaacaa tgaagcaaaa gctttggcta
    13681 taatatataa tgcgtgggag gaat
//
LOCUS       DAOPUE010000048        13506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4661_length_13506_cov_10.509851, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000048 DAOPUE010000000
VERSION     DAOPUE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4661_length_13506_cov_10.509851"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKH42_05780"
     CDS             complement(<1..869)
                     /locus_tag="PKH42_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19975.1"
                     /translation="MVLTSLHYLYMIMVVIILVIMLMKKEIVIPCIVGIFAMGFLYSG
                     SAVFAVQSIFNTIIYSGNEFWGIILIISLVVAMSKSLQAVGADALIMSPIKKIMVNQT
                     MAFWGIGVAMMIISWLVWPSPAVPLIGAVLLPAAVATGLPVIYAAVAMNLFGHGIALS
                     SDFFIQGAPTITAAGAGIEVTEIIRASIPIWLTMSAVTIATAFFLMKRDLKKSSQLLP
                     SADNNFKHEEITQSKTAKYVAVLTPIAFLIDIAAMLKFNLAGGDATALVGGTAIIIML
                     LTVLTSFKLRDGF"
     gene            complement(977..>1490)
                     /locus_tag="PKH42_05785"
     CDS             complement(977..>1490)
                     /locus_tag="PKH42_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19976.1"
                     /translation="NVRKLFTESFTSNGYISYTEKFFEENEVWAVVGNDTNYSSVFLE
                     RILNEAIKKGSTAECYYRPLFPKKLQHIYLPEKKLIIVTAENHMNEKYKEVFDIHCII
                     DSESIKTRVSEIEKNLHLYDLLIRNALDKLSETKKLHELLEVFYVDSMDFNLVNECFE
                     KIVRGIGTQI"
     assembly_gap    1491..1590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1591..2202)
                     /locus_tag="PKH42_05790"
     CDS             complement(<1591..2202)
                     /locus_tag="PKH42_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOA19977.1"
                     /translation="MKEKHIFAGNNTSEGFFSYFDYLLKPEEAEHIYILKGGPGVGKN
                     RFMKKFADEMEKKNYSIEYIHCSSDNASLDGILIPELKILIVDGTSPHTIDPVIPGAV
                     DEIINLGAFLNVQKLNKHKTAIIQTNKAKSNKYLSAYRYLKAAGIIYNEISSIYDLYV
                     DVEKFNEMCEKAITGLFAHSSDKKSDNVRKLFTESFTSNGYISY"
     gene            2430..2618
                     /locus_tag="PKH42_05795"
     CDS             2430..2618
                     /locus_tag="PKH42_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOA19978.1"
                     /translation="MQGKVQKMDHHNPGIKCVVNTCYYYDEGDHCKAQKIEVQRRNAT
                     SSHETDCATFTKNDQSMT"
     gene            2790..3431
                     /locus_tag="PKH42_05800"
     CDS             2790..3431
                     /locus_tag="PKH42_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOA19979.1"
                     /translation="MKKITKIEYQKKNKERFNIYIDDEYGFAVDISILIKYSLKKGME
                     LDDALIDEILLSEEEISVYNYGISVLSRYFKSEYELRLKMKNKGFNPQLIDNAISILK
                     EHKYLDDERYCEMFINDKINISKHGVRKIKEALYYKGIDKEIIEEKIKCISAESEEER
                     ALLLGEKKLLNIKENDNRKKMSKLSNYLLGKGFEYETVNKTLRKLLNADNDFY"
     gene            3446..4333
                     /locus_tag="PKH42_05805"
     CDS             3446..4333
                     /locus_tag="PKH42_05805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA19980.1"
                     /translation="MKEFADYISDAFVHCRMTYNDGKFDIEALYANKQLEHITNKTMD
                     QLIGSKMTEVFPNLADSIFDWPKILCEAAMTSEHSVIEQYVNAFDKFVKFNIFGFKDD
                     TFYMTIQDLTHKREIRRLILEKDRQIEHLENEIKSRASIEPLTNIYNFQFITDCMKHS
                     IQSYKEEGTAFCVFLIDIDDFKEFNRKYGIETGDMVLQDVARILTLAARKIDVVGRYG
                     NDKFIMILNNVELDIAKIIVEKVKMEIGRYDLNISNNTIHASGAVVEYRGETLEEFVE
                     KAEELLKKAQAMGKGKILS"
     gene            complement(4355..5527)
                     /locus_tag="PKH42_05810"
     CDS             complement(4355..5527)
                     /locus_tag="PKH42_05810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOA19981.1"
                     /translation="MRNYIDLNKVCPVIKGRLSSSSNNIIPVIISYKSGKKVKEGQIS
                     SLSNRLRHNLPIINGCACEMSTESILKLTAEPDVEFISYDSKVFAVMEEARKTIGADY
                     SLNTKYTGKDVTVAIIDTGVAPHADLLYPENRIVGFKDFVNNRSKYYDDNGHGTHCAG
                     ILAGSGYSSKGKYRGIAPEANILSIKVLDENGNGNTSDILSTVQWIIETKEVYKTKII
                     NFSLGAIAQYKERRDPLVKAANKAIEENLIVVAAVGNSGPMHNTILSPATSRYVISVG
                     SLNDRGTSHFENETIAEFSSRGPTLDRVRKPDLIAPGVDIISLSNKNLSGYTALSGTS
                     MSAPMVSGAAALLLNKNPNYTHFDIKRILLNSCSKIKASSYEQGAGVLDMRRIFSQ"
     gene            5755..6438
                     /locus_tag="PKH42_05815"
     CDS             5755..6438
                     /locus_tag="PKH42_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA19982.1"
                     /translation="MPKILVVDDERPIAEIIKYNLQKEGFEVQTAYDGDEAIKAVHNF
                     QPELIILDIMLPKKSGFDVLKEIRMEYVMPVIMLTAKDEENDKVTGLELGADDYITKP
                     FSNKELIARVKANLRRVKLSKVEEEMKSKIIAVGNLTIDMNTYEVFKDGEIIDLTNRE
                     FDLLKYLFQNSDRVFNREHLLKEVWGYEFGDLRTVDVTVRRLREKIETEDEKYIITKR
                     GTGYYFKSK"
     gene            6473..8254
                     /locus_tag="PKH42_05820"
     CDS             6473..8254
                     /locus_tag="PKH42_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA19983.1"
                     /translation="MFKSIRWRFILIYFLLVFLAMSIVGIYIVNQLEGIQLNMNIKNM
                     DARIRSIIQSYSSLNTGNWDENIEEIQKSIGTVQVGYNENIYVILNDVNRTIIAGSVE
                     ESIGISAFNYNKIDNYILTKSMDGTPYHIAPAEQFEDTENQRFYYHMSFPVQTEGNIK
                     GYIYLVNNIDYIYDTVDESKQIMTQATIIALTLTIFLGLILSTSITGPIKELTIKARQ
                     MSLGDFEQKVNVKSDDEIGQLGNMFNYLIDQLKKSMSSLQQEKSKMETTFTYMADGVL
                     TVDLSGNIIHANPVAKNILSLKNADEKFDDVIVNLKDSISIDYIKSKGYRGTDLAEKN
                     GETYNIDYAPFMNNKNEIGGVILVFKNITEQHRIDKLQKEFVANVSHELKTPITTIKS
                     YAETLLDGALEDKQISEEFLNVINSESDRMTRLVSDLLRLSKMDYEQTNWNKERINLL
                     DMVNIVIKKLQIQIKEKNINIHLNIPDDINIMFDRDGFEQILLNIAGNAVKYSYEKGN
                     VWISARRSKKNIVVSIKDDGQGIPTEDQDRIFERFYRVDKARARGLGGTGLGLSIAKE
                     IAEAHNCTIAVNSRIDEGTEMIITIPE"
     gene            8265..9668
                     /locus_tag="PKH42_05825"
     CDS             8265..9668
                     /locus_tag="PKH42_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19984.1"
                     /translation="MLNIKIKNSILLVMVLICIYLSSNVWLKLPEFFTPKAYEDKRAE
                     EAEADIWKVLRPVKNVIKYDDNYTVLYSDNKGLWQEALNVIYDAFNATNSTISETAAF
                     PAEYLKFDFNTNIPVEIFTTHMKIENNNINNTVKNIKNVTIDLKDNNSIYIYNGENNI
                     KIKSNQINSKKLTDIVLSINFENKTKYSMPEKVGDETIQVPVPLEQSALNPVFVQSEL
                     DVFDIDRINEIAKDYFKNDYDYVRKSVEVSGNLVYVYRTEKILKINEDGLLDFYDASI
                     EPENSPNVYKSFAAAVNFIEEFLGFPENGYLSNVEKILLEGNEGYRFTFSYNILERPI
                     LFSRVRENAALQIDVAGNNVISYKRFIRNIDRTQMDKMAEIEVTPAIEVIRNNIDTSA
                     GEENADTVPGEGQEEAVQLKPIKPEMIKEINNIYLGYFDLSRISREQVLRVVWVIETN
                     EKTFIFNAISGLLIEEW"
     gene            9681..10511
                     /locus_tag="PKH42_05830"
     CDS             9681..10511
                     /locus_tag="PKH42_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19985.1"
                     /translation="MDWNKTNTILITAFIILNVFLFVSSKNIRNAEHKPNYDKEFAQE
                     VAYLLEEKNIKINVEIPNNIYILPVLETEYEIIKIDSNLLTNFLNERIEPFEDVYKYT
                     NSKGETLEIIDGKKLRLTLRNKVDDKINEDSKIQEEINNFLKEKNIDASGYVESFKHT
                     SENECIYAYTLKYNDYSIDNSYMYFFVDNEGIYKFEMQRIISVDEIPEKIRTIYAIEA
                     LPRILTYPEIKNKEIIKIEMTYYSAEDENWHNIERINSDPTWKVIFSDGTQIHLPGIE
                     "
     gene            10532..11791
                     /locus_tag="PKH42_05835"
     CDS             10532..11791
                     /locus_tag="PKH42_05835"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOA19986.1"
                     /translation="MSKFVIEGGTKLKGKVNIGGFKNAAVAIIPATLLCPERCIIENV
                     PKIQDVNVFLEILRELGAEADYINDTSIYVDASNVKNCYLENGLSKKMRASYYLLGAG
                     LGRFNGASCLMPGGCDIGERPIDQHIKGFEALGGKVTLTPDGRISVQADRLVGAKIYL
                     DVVSVGATINIMLAAVYAEGRTIIENAAKEPHIVDTANFLNSMGADIKGAGTDVIRIN
                     GVKELKGCTYKVIPDQIEAGTYMIAAAATKGDIIIDDIIPKHMEPITAKLKEMGMGIE
                     EYGDSMRVYYEKELVPVNIQTLPYPGFPTDLQQPMTVLLASLCGESTVVEGVSQDRFR
                     YVEELKKMGACIEFTKKQARIKGTKNLRGTVVQATDLRAGAALIIAGLIADGLTEVTN
                     IQHIDRGYEHIENKFMNLGAKIKRVED"
     gene            11811..12956
                     /locus_tag="PKH42_05840"
     CDS             11811..12956
                     /locus_tag="PKH42_05840"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOA19987.1"
                     /translation="MNKAVLIPDSFKGTLSSIEICTIISDKIYKHFPHCHVVSIPVAD
                     GGEGSVDCFLSALGGEKIVETVSSPYFEDMESFYGLIDDTAVIEMAACAGLPLVEDRK
                     NPSLTTTYGVGQLIMAAAKKRCKKIIVGLGGSSTNDGGCGAAAAVGVKFYDKKGNEFI
                     PIGKTLIDIDRIDLSHKAKELDNIEIITMCDIDNPMYGTTGAAYIFGPQKGADEKMIL
                     ELDKGLKNLCDVIERETGKDLKDVPGSGAAGAMGAGMIAFFNSVLQMGIETVLDTVKF
                     DEIIEDADMIFTGEGRIDSQSLRGKVVIGVGKRAKNKNIPVTVIAGGAANNIDDAYDM
                     GVTSIFTINRLPEDFEISRHKSKENLEATIDNILRLIKSVYIERGVQ"
     gene            12953..>13506
                     /locus_tag="PKH42_05845"
     CDS             12953..>13506
                     /locus_tag="PKH42_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA19988.1"
                     /translation="MNTENIKIAILGFGNVGRAFAKLLTEKREEIFNKFSLNVDVVAI
                     STRTKGCLVNENGIDLKEALNDIEKFNHFDNKRSDYKDISSMEIAENVDYDILFELTP
                     LEIFSGQPAIDHIKAALSRKKHAVSANKGPIAWAYESLRDLAEKNKVLFYHETTVMDG
                     TPLFNLVDETLKFCKIKEVKGILNS"
BASE COUNT         4861 a         2001 c         2621 g         3923 t
ORIGIN      
        1 tcaaaaccat ctcttaattt aaagcttgtt aaaacagtta agagcattat gattattgcg
       61 gttcctccta caagtgcggt tgcatcgccg cctgccaaat tgaattttaa cattgccgca
      121 atatcaatta aaaatgctat cggagttaaa acagccacat attttgctgt cttgctttga
      181 gttatttctt catgtttaaa attattatct gcagaaggta aaagttggga agactttttc
      241 aggtctcttt tcatcaggaa aaatgcagtt gctatagtca ctgcagacat tgtaagccat
      301 atgggaattg atgccctaat aatttcagtt acttcaatgc ccgccccggc agcggttatg
      361 gtaggtgctc cttgtataaa aaagtcactg gaaagtgcaa taccatgacc gaataaattc
      421 atggcaaccg ccgcataaat aacaggcagg cccgtggcaa ctgcagcagg cagaaggact
      481 gcccctatta agggaactgc aggactgggc caaacgagcc aggatattat catcattgcc
      541 acacctattc cccaaaatgc cattgtttga ttaaccatta tttttttgat tggtgacata
      601 attaacgcat ctgcaccaac cgcctgcaag gacttagaca tggcgacaac caatgaaatt
      661 ataagtataa taccccaaaa ttcattgcct gaataaataa ttgtgttaaa tatagactga
      721 acagcaaata ccgcactgcc agagtaaagg aaacccatgg caaaaatacc gacaatacat
      781 ggaattacaa tttctttttt catgagcata ataacaagaa taattactac cattatcata
      841 tataagtagt gaagagaagt taaaaccata tgaaacctcc ttggaattaa gcagaggaac
      901 attccttcaa gaaaatttta atagtgtgtc cccctgcctt atgtaattgt gtcctcttac
      961 atataaattg gcaccattat atttgtgtac ctattcctct gactattttt tcaaagcatt
     1021 cattaacaag gttaaaatcc atggagtcaa cataaaatac ttctaaaagt tcatgcaact
     1081 tttttgtttc ggaaagctta tctaaggcat ttcttattaa taaatcatat aaatgaaggt
     1141 ttttttcaat ttcagaaaca cgtgtcttta tactttcaga atctattatg cagtgaatat
     1201 caaaaacctc cttatatttt tcattcatat gattttctgc agtaacaatt ataagttttt
     1261 tttcaggcaa gtatatatgc tgcagctttt tcgggaatag aggtctgtaa tagcattctg
     1321 cagtagatcc cttttttatt gcttcgttta aaattctttc caggaatacg gaagaatagt
     1381 ttgtatcatt acctacaacc gcccacactt cattttcttc aaagaacttt tcggtatagc
     1441 ttatatatcc gtttgacgta aaagattcgg taaacagttt tctgacattg nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atagcttata tatccgtttg acgtaaaaga
     1621 ttcggtaaac agttttctga cattgtcgct ttttttatca gatgaatgag caaacagtcc
     1681 ggtaattgct ttttcgcaca tttcattaaa tttttcgaca tcaacataca aatcataaat
     1741 cgagctgatt tcgttataga taatacctgc agcttttaaa tacctgtaag cacttaaata
     1801 tttatttgat ttagctttat tggtttggat tatagctgtc ttatgtttat ttagtttttg
     1861 aacatttaaa aaggctccga gattaataat ttcatcaact gctcccggga tgacagggtc
     1921 gatggtatga ggtgatgtac cgtcaacaat aagaattttt agttccggaa tcaaaattcc
     1981 gtcaagactt gcattgtcgc ttgagcagtg tatgtattct attgaatagt tctttttttc
     2041 catttcatct gcaaattttt tcatgaaacg atttttgcct actcctggtc ctcctttaag
     2101 aatataaatg tgttcagctt cttccggctt taatagataa tcaaaatagc tgaaaaatcc
     2161 ttcagatgta ttgttgccgg caaaaatatg tttttctttc attttctacc tcctcaatgt
     2221 tattccatta tatgaacgat ttctgttctt gttaaaatta attctttttt attattcctg
     2281 attacatcct gaaagattaa gcataaggag aaattcttct ttagatttgt atttatttgt
     2341 aataaaaaaa tagatattat aaaaaaaata ttaaagggca taataatttt gaaagttttg
     2401 actttcaata acaaaaaagg agacatatta tgcaaggaaa agtacaaaaa atggatcatc
     2461 ataatcccgg catcaaatgt gttgtaaata catgttatta ctatgatgaa ggggaccact
     2521 gcaaggcaca gaagattgaa gtgcaaaggc gcaatgcaac ttcatcacat gaaacggatt
     2581 gtgcaacatt tactaaaaac gaccaaagca tgacataaag gtgccataac aggcaagata
     2641 atttatccga aaaattgtcc ttttaagagg acaatttttt tgggtattat ttcggatact
     2701 tttttatctt agatatgccc atataatatt gctttataaa tattgatatt cttcaaaatc
     2761 tgtgttaata tgtagaaaat gagggaaata tgaaaaagat taccaaaatt gaataccaga
     2821 agaaaaataa agaaagattc aatatatata tagatgatga atatgggttt gctgttgata
     2881 taagcatatt gattaaatat tcgctcaaaa aaggtatgga attagatgat gctctcattg
     2941 atgaaatatt gttatcagaa gaagaaataa gtgtatataa ttacggaatt tctgtgcttt
     3001 caagatattt taaaagcgaa tatgaattaa ggcttaaaat gaaaaataaa ggctttaacc
     3061 ctcagttaat cgacaatgcc atcagcatac ttaaagagca taaatactta gatgatgaaa
     3121 gatattgtga aatgttcata aacgataaaa tcaacatttc caaacatggt gtgcgtaaaa
     3181 ttaaggaagc tttatattac aagggcattg acaaagaaat aatagaagaa aagataaagt
     3241 gtatttcagc agagagcgaa gaagaaagag ctttattatt gggagagaaa aaacttttga
     3301 acataaaaga aaatgataac cgcaaaaaaa tgtcaaagtt gtcgaattat ttgttaggaa
     3361 agggctttga atatgaaacg gtaaataaga cactaagaaa gctattaaat gcagacaatg
     3421 atttttacta aagggggcta tacttatgaa ggaatttgct gattatatat ccgatgcatt
     3481 tgttcattgc aggatgacct ataatgatgg aaaatttgat attgaagctc tctatgcaaa
     3541 taagcagtta gagcatatta ccaataaaac aatggaccag ttaatcggaa gtaaaatgac
     3601 ggaagtattt cctaacctgg cggattcaat atttgactgg ccgaaaattt tatgcgaagc
     3661 tgctatgaca agtgaacact cagtaattga acaatacgtc aatgcctttg ataagttcgt
     3721 aaaattcaat atatttggat ttaaagatga tactttttat atgacaattc aggatttaac
     3781 tcataaaaga gaaatcagga gattaatact tgaaaaagac agacaaatcg aacatttgga
     3841 aaatgaaatc aaatcaagag caagcataga gccccttacc aatatatata actttcaatt
     3901 tattaccgac tgtatgaaac acagcataca aagctataag gaagaaggaa cggcattttg
     3961 cgtattcttg atagatattg atgactttaa agagtttaat cgtaaatacg gcattgaaac
     4021 aggagatatg gttttacaag atgtagcccg catactcact ttggctgcaa gaaaaataga
     4081 tgtggtgggc aggtatggaa atgataaatt tattatgata ttgaacaatg tagaacttga
     4141 tattgcaaaa ataattgttg aaaaagtaaa aatggaaatc ggcagatatg atttgaatat
     4201 aagcaataat actattcatg caagcggagc cgttgtggaa tacaggggcg aaacattgga
     4261 agaatttgtt gaaaaagctg aagaattact taaaaaagca caagctatgg gaaagggaaa
     4321 aatattgtca taagggagca ttgcgctccc tttattactg tgaaaaaatg cgtctcatat
     4381 ctagaactcc tgctccttgc tcatatgaag atgcttttat ttttgagcat gaattaagga
     4441 gtattctctt aatgtcgaaa tgggtgtaat tgggattttt gtttaaaagt aaagcggcag
     4501 ctccggatac cattggggcg gacattgatg taccgctcaa tgcggtgtag ccgcttaagt
     4561 ttttattgga taaagatatt atatcaactc cgggagctat taaatccggt ttgcggactc
     4621 tgtccaatgt agggcctctg cttgaaaatt cagcaattgt ctcattttca aaatgagacg
     4681 tgcccctgtc atttaatgag cctacggaaa ttacatacct gctggttgca ggagataata
     4741 ttgtattgtg cataggcccg ctgttgccaa cggcagcaac cactatgaga ttttcttcaa
     4801 ttgctttgtt tgccgccttt accaatggat ctctcctttc cttatactga gcgattgcgc
     4861 cgagagaaaa attgattatt ttagttttat aaacctcctt ggtttcaata atccattgaa
     4921 ctgtagatag aatatccgat gtatttccgt ttccgttttc atcaagaacc ttgattgata
     4981 gtatattggc ttccggggct attcctctat atttgccctt ggaagaataa ccgcttcctg
     5041 caagtattcc tgcgcaatgt gttccatgac cgttatcatc gtaatacttg gagcgattgt
     5101 ttacaaaatc cttaaagcca actatcctgt tttcgggata taacaaatca gcgtgagggg
     5161 cgacacctgt atcaattatt gcaacagtta catcttttcc cgtatattta gtatttaggg
     5221 aatagtcagc tccgattgtt tttcttgcct cctccattac cgcaaatact tttgaatcat
     5281 atgatataaa ttcaacatcg ggctcagctg ttaatttaag aattgattca gtgctcattt
     5341 cacaagcaca accgttgata atgggcagat tgtgccttaa tctgtttgat agcgaactga
     5401 tttgcccctc ctttactttt ttgccggatt tataacttat gataacaggt ataatattat
     5461 tgctgcttga agaaaggcgt ccttttataa ccggacacac tttatttaaa tctatataat
     5521 ttctcataaa attcacctac ctaaattaat ttatgctttt aaataaagat ggtgttcatt
     5581 tttgaaaaac atgttataat attcatgaat aatataatgg gaaagatatc ttcctcctta
     5641 ggaggcgggt atatgtgaaa ttcaggcggg tatatcccgc ctcgttgaaa cggtgaaatt
     5701 gcacggcaat ttcttcggat gtttaaagac agtaattaaa ataggggtga aataatgcct
     5761 aaaatattag tagtggatga tgaaagacct atagccgaaa taattaaata caatcttcaa
     5821 aaagaaggct ttgaagtgca gacagcctat gacggggatg aagctataaa ggcagtgcac
     5881 aatttccagc ctgaattaat aatacttgat attatgctgc caaagaaaag cggatttgat
     5941 gttttaaaag aaatacgcat ggaatacgta atgcctgtta ttatgcttac tgcaaaagat
     6001 gaagaaaacg acaaggttac ggggcttgaa ttgggagcag acgactatat aaccaagcct
     6061 ttcagcaaca aggaattaat tgcaagagtc aaggctaatt tgagaagggt gaagctttca
     6121 aaggttgaag aagaaatgaa atcaaagata atagcagtag gaaatctaac cattgacatg
     6181 aacacatatg aagttttcaa agatggtgaa ataatagatc ttacaaacag ggaatttgac
     6241 cttttaaaat atttgtttca aaattcagac agagtattta acagagagca ccttcttaag
     6301 gaagtatggg gatatgaatt tggtgattta agaacagtcg atgtgacagt gagaagatta
     6361 agagaaaaaa tagaaacaga agatgaaaaa tatataatca caaaacgagg gacaggatat
     6421 tattttaaaa gcaaatgatg ccaaattttg ctgcgtattc taaggagaaa aaatgttcaa
     6481 gagcataaga tggagattta ttttaattta ttttttgttg gtttttcttg ctatgtcaat
     6541 agtcggcatc tatatagtca atcagcttga gggaattcag cttaacatga atataaaaaa
     6601 catggatgct cgaattcgct caattattca atcctacagc tcattaaata ccggtaactg
     6661 ggatgagaat attgaagaaa ttcaaaaaag cataggcact gtacaggtag gttataacga
     6721 aaatatatat gtaatcttaa atgatgtaaa cagaacaatt attgcaggca gtgtagaaga
     6781 atcgattggc ataagtgcct ttaactataa taaaatagac aattatatac tgactaaatc
     6841 catggatgga actccttatc acattgcacc ggcagaacaa tttgaagata ccgaaaatca
     6901 aaggttttat tatcacatgt cttttccggt gcagacggaa ggaaacatca aaggttatat
     6961 atatttagta aataatattg attatattta cgacacggtt gacgaatcaa aacaaattat
     7021 gacacaagct accataattg ccttaacctt aacgatattt ttaggactga tactttcaac
     7081 aagcataacg ggaccaatca aggagctcac tataaaggca aggcagatgt cattaggcga
     7141 ttttgagcag aaggtaaatg taaaatcaga tgatgaaata ggtcaattgg gcaatatgtt
     7201 caattacctt atagaccagc tgaaaaagtc aatgtcaagc cttcagcagg aaaaaagcaa
     7261 gatggaaacc accttcacat atatggcaga tggagttttg acagttgatt tatcaggtaa
     7321 tattatacac gcaaaccccg ttgcaaaaaa tatattatca ctaaaaaatg ccgatgagaa
     7381 atttgatgat gtaatagtaa atctcaaaga cagcatatca attgactata ttaagtctaa
     7441 aggttatcgc ggcacagact tggcagaaaa aaacggagaa acttataata tagactacgc
     7501 acctttcatg aacaacaaaa atgaaatagg gggcgtgatt cttgtgttta aaaatattac
     7561 cgagcagcat cgaatagata agcttcaaaa agaatttgtc gcaaatgtat cgcatgaatt
     7621 aaaaacgccg atcactacca taaaaagtta tgcagaaact ctattggacg gtgcattgga
     7681 agataaacaa atatccgaag aatttctgaa tgtcataaat tccgaaagcg ataggatgac
     7741 aaggcttgtc agcgatttat tgcgcctttc aaagatggat tatgagcaga caaattggaa
     7801 taaagaaaga atcaatttgc tggacatggt taatatagtc atcaaaaaac tgcaaattca
     7861 aataaaagaa aaaaatataa atattcattt aaatattccg gatgatatta atataatgtt
     7921 tgacagggac ggatttgagc aaattctttt gaatattgca ggaaatgcag ttaaatattc
     7981 atacgaaaag ggaaatgtat ggattagcgc tagaaggagc aagaaaaata tagtcgttag
     8041 catcaaagat gacgggcagg gtattccgac agaagaccag gatagaattt ttgaaagatt
     8101 ctatcgtgtt gataaagcaa gagccagagg cttgggagga accggccttg gactttcaat
     8161 tgctaaagag attgcagaag cccacaactg tacaatagcc gtcaacagcc ggatagatga
     8221 gggaactgaa atgattatta caattcctga ataaagagga cactatgtta aatataaaaa
     8281 taaaaaattc aatattattg gtcatggtac tcatatgtat atacttaagc agcaatgtat
     8341 ggctaaaact tcctgaattc ttcacaccta aggcatatga agataaaaga gcggaagaag
     8401 ccgaagcaga catatggaaa gtattgagac ctgtaaaaaa tgtaattaaa tatgatgata
     8461 actatactgt tttgtattca gataataaag ggttgtggca ggaagcatta aatgtaatat
     8521 atgatgcttt taatgctaca aatagcacca tttcggaaac agcggcattt ccggctgaat
     8581 atttgaaatt tgatttcaat acgaatatcc ctgtggaaat atttacaacc catatgaaaa
     8641 ttgaaaacaa caatataaac aatactgtaa aaaatataaa gaatgttacg atagatttga
     8701 aagataacaa ttcaatttat atttacaatg gcgaaaacaa tataaaaatt aagagtaatc
     8761 aaattaattc caagaaatta acagatattg tgctatctat aaattttgaa aataaaacaa
     8821 aatattccat gccggaaaaa gtaggggatg aaacaatcca ggtacccgtt cccttagagc
     8881 agtcagcgct gaatccggta tttgttcagt cggagcttga tgtatttgat attgacagaa
     8941 ttaatgaaat tgccaaagat tattttaaaa atgattacga ctatgttaga aaatctgttg
     9001 aagtaagcgg gaatttggta tatgtttaca ggaccgaaaa gatattaaaa attaacgagg
     9061 atggactctt agacttctac gatgcatcaa tcgagccgga aaacagtccg aacgtttata
     9121 aaagctttgc tgcagccgta aattttatcg aagaatttct ggggtttccg gaaaacgggt
     9181 atttgagcaa tgtagaaaag attctcttgg aaggaaatga aggttatagg tttacattct
     9241 cctacaatat attggaaagg cccatattgt tcagcagggt aagagaaaat gctgctcttc
     9301 aaattgatgt tgcaggaaac aatgttattt catacaaaag gtttatcaga aatattgata
     9361 gaacacagat ggacaaaatg gctgagatag aggttactcc tgctattgaa gtgataagaa
     9421 acaatatcga cacttcggcc ggtgaagaaa atgcagatac agtacccggg gaaggccagg
     9481 aagaagctgt acagcttaaa cccattaagc cggaaatgat taaggagatt aacaatattt
     9541 atcttggata ttttgattta tctcgaattt caagggaaca ggttttaagg gtggtatggg
     9601 ttattgaaac aaatgaaaaa acatttatat ttaatgccat atcaggtctt ttaattgaag
     9661 aatggtaaca ggtgaggcaa atggattgga ataaaacaaa cacaatatta ataacagctt
     9721 ttataatttt aaatgttttt ctttttgtgt cttccaagaa tattagaaat gcagagcaca
     9781 agcctaatta tgataaggaa tttgcccaag aggtagcata tttattagaa gaaaaaaata
     9841 taaaaattaa tgttgaaata ccgaataata tatatatact gcctgttttg gaaacagaat
     9901 atgaaataat taagatagat tcaaatcttt tgacaaattt cttaaatgaa agaattgagc
     9961 cttttgaaga tgtgtacaaa tacacgaaca gcaaaggaga aactttagaa ataattgacg
    10021 gcaagaaatt aagactcaca ttacgcaata aggttgacga taaaataaac gaagattcaa
    10081 aaatacaaga agaaataaac aattttttaa aagaaaaaaa tattgatgca tccggatatg
    10141 ttgaaagttt caaacacact tccgaaaatg aatgtattta tgcatatacg ctaaaatata
    10201 atgactattc aatagacaat tcctacatgt atttttttgt agataatgaa ggaatttata
    10261 aatttgaaat gcagagaata atttcagttg atgaaatacc ggaaaagatt cgtacaatat
    10321 atgctattga agcattaccg cgaattttaa cttatccgga aataaaaaac aaagaaatta
    10381 ttaaaattga aatgacatat tatagcgctg aagatgaaaa ttggcataat attgaaagga
    10441 taaattccga cccaacgtgg aaagttattt ttagcgatgg aacacaaata catctgccgg
    10501 gaattgaata atatatattg cgaggaagag tatgtcaaaa tttgtaatag aaggcggcac
    10561 aaagcttaaa ggaaaagtaa atattggggg atttaaaaat gcagctgttg caattatacc
    10621 ggctacgtta ctttgtcccg aaaggtgcat tattgaaaat gtaccaaaaa tacaggatgt
    10681 aaatgtattt ttggaaatat tgagagaatt aggtgcagaa gcggattata tcaatgatac
    10741 gtcaatatat gtggacgcat caaatgtaaa aaattgttat ttggaaaacg gattatcaaa
    10801 aaaaatgaga gcctcatatt acttgcttgg agccggattg gggagattta atggagcatc
    10861 gtgcttgatg cccggcggct gtgatatagg cgaaagacca attgaccagc acattaaggg
    10921 gtttgaggca ttaggaggaa aagttactct tactccggat ggaagaataa gtgtacaagc
    10981 agacagatta gtaggagcaa aaatttatct tgacgttgta agcgttggag caaccataaa
    11041 cattatgttg gcagcagtat atgccgaagg aaggacaata attgaaaatg cggctaaaga
    11101 gcctcatatt gttgatacgg caaacttttt aaattcaatg ggtgcagata ttaagggcgc
    11161 aggaacggat gtaatacgta taaacggtgt aaaggaatta aaaggctgca cttacaaggt
    11221 tatacctgac caaattgaag caggtacata catgattgct gctgctgcca caaaaggaga
    11281 tataataatt gatgatataa ttcccaaaca tatggagcct attaccgcaa agcttaaaga
    11341 gatgggaatg ggcatagaag agtacgggga ttccatgcgt gtttattatg aaaaggaatt
    11401 ggttccggta aatatccaga cacttccgta tcccggattc ccaacagatt tgcagcagcc
    11461 aatgactgtg cttttagctt ccttatgcgg agaaagtaca gtagttgaag gggtatccca
    11521 ggacagattc agatacgtgg aagagttgaa aaaaatgggc gcatgcatag aatttacaaa
    11581 gaagcaagct aggattaaag gtacgaaaaa tttaagggga acagttgtac aagctacgga
    11641 tttaagagcc ggagctgcat tgattatcgc aggtcttatt gctgacggat tgactgaagt
    11701 gacaaatata cagcatatag acagaggata tgaacacatt gaaaataaat ttatgaattt
    11761 aggagccaaa attaaaagag tagaagatta ataacagggg gaggaaacat ttgaataaag
    11821 cagtattaat tcctgattca tttaaaggaa cattgtcgtc cattgaaata tgcactatta
    11881 tttctgataa aatatataag cattttccgc actgccatgt ggtatcgatt ccggttgctg
    11941 acggaggcga agggagtgtg gattgttttt tgtcggcatt aggcggtgaa aaaatagttg
    12001 aaactgtaag ctcaccctat tttgaagata tggagtcatt ttacggcttg attgatgaca
    12061 ctgcggtaat tgaaatggct gcatgtgcag ggttacccct ggtggaagac aggaagaatc
    12121 caagccttac gacaacttat ggagtggggc agcttataat ggcagcagca aaaaaaagat
    12181 gtaaaaaaat tattgtcggc ttaggaggct caagcaccaa tgacggtgga tgcggtgcag
    12241 ctgcagccgt tggtgttaaa ttttacgata aaaaagggaa tgaatttata ccaataggga
    12301 aaacactaat agatatagat agaattgatt tgtcccataa ggcaaaagaa ttggataata
    12361 ttgaaataat aacaatgtgt gatatagaca acccaatgta cggaactacg ggagctgcat
    12421 atatattcgg tcctcaaaaa ggagcggatg aaaaaatgat tttggagctt gacaaagggt
    12481 taaaaaatct ttgtgatgta atcgaaagag aaacaggcaa agatttaaaa gacgttccgg
    12541 gcagcggagc agccggagct atgggtgcag gaatgattgc gttttttaac tctgtacttc
    12601 aaatgggaat tgaaacagtc ttagatacgg taaaatttga tgaaataatc gaggacgctg
    12661 atatgatttt taccggcgaa ggcagaattg actctcaaag cttaagaggc aaggttgtta
    12721 taggagtggg aaaacgagca aaaaataaga atattcccgt aacagtcatt gcaggaggag
    12781 cagcaaataa tattgatgat gcctatgata tgggagttac ttcaatattt acaatcaaca
    12841 gactgcctga ggactttgaa ataagcaggc ataaatccaa agaaaactta gaagcaacaa
    12901 tagataatat tctcagactc ataaaaagcg tatatataga aaggggagtg caatgaatac
    12961 cgaaaatatt aaaattgcaa ttcttggatt cggtaatgta ggaagggcat ttgcaaagct
    13021 gctgactgaa aaacgagaag aaatattcaa taaattcagc ttgaatgtcg atgtggttgc
    13081 catttcaacc agaacaaaag gatgcttagt aaatgaaaat ggtattgatt taaaggaagc
    13141 attaaatgat attgaaaagt tcaatcattt tgataataaa agaagtgatt ataaagatat
    13201 cagctcaatg gaaattgccg aaaatgtaga ttatgatatt ctttttgaat taactcctct
    13261 tgaaatattt tcaggtcagc cggctataga ccatataaaa gcggcactta gcagaaaaaa
    13321 acacgcagtt agcgcaaaca aggggcctat tgcttgggca tatgaatcat tgcgtgattt
    13381 agcagagaaa aacaaggttc ttttttacca tgaaacgact gtaatggatg gcaccccctt
    13441 atttaactta gttgatgaaa cacttaaatt ctgtaaaata aaagaggtaa aaggaatatt
    13501 aaattc
//
LOCUS       DAOPUE010000049        13176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4808_length_13176_cov_11.493331, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000049 DAOPUE010000000
VERSION     DAOPUE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4808_length_13176_cov_11.493331"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /gene="rpsR"
                     /locus_tag="PKH42_05850"
     CDS             complement(<1..203)
                     /gene="rpsR"
                     /locus_tag="PKH42_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOA19989.1"
                     /translation="MSSRQSGKDINDKNEKYSQRRKPRKKVCQFCQEKATHIDYKDLG
                     KLKKYTTERGKILPRRITGACAK"
     gene            complement(228..686)
                     /locus_tag="PKH42_05855"
     CDS             complement(228..686)
                     /locus_tag="PKH42_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOA19990.1"
                     /translation="MNNVVLIGRLARDPDLRFIPSTGMAVAKFPLAVDRGLYGEKKQE
                     AINQGKQTADFINITVFGKTAELCANYLTKGRQCAIQGRIQTGSYTTQTGEKRYTTDV
                     IADRVEFIGSKEQSSSPMGNAVQPDSSFFNDFPDEDNDIFEPVDDDDIPF"
     gene            complement(703..993)
                     /gene="rpsF"
                     /locus_tag="PKH42_05860"
     CDS             complement(703..993)
                     /gene="rpsF"
                     /locus_tag="PKH42_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOA19991.1"
                     /translation="MRKYEGAFILLSNLEEDVRNAEIEKIQSIITERQGTIEKVNEWG
                     QRRLAYEINKKREGYYIFIEFTAGADAVDEVDRICKISDNFIRHMITVVEEK"
     gene            complement(1084..1245)
                     /locus_tag="PKH42_05865"
     CDS             complement(1084..1245)
                     /locus_tag="PKH42_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxH/CxxC protein"
                     /protein_id="HOA19992.1"
                     /translation="MKEMYEEIFACMEHVEQAIDDYVNSKEAAPQLIKTDKKEKCSYC
                     KKNAEYIIK"
     gene            complement(1253..2041)
                     /locus_tag="PKH42_05870"
     CDS             complement(1253..2041)
                     /locus_tag="PKH42_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA19993.1"
                     /translation="MKLKFCSLYSGSSGNCQYIKTKNTTIIVDAGLSGKRIQQEIANI
                     GEDPNKIDAIFITHEHIDHIQGAGIMSRRLNIPIYANEETWEAMKSLIGDIKSCNIKI
                     LDESTQIGDLTIKSFDISHDAAHPVGFNIFYENKKISLVTDTGCFNGTITNSIMDSDL
                     LLVESNHDEDLVIIGPYPWPLKRRVLGEFGHMSNEAAGNLIKKVIKKGTEIILLGHLS
                     KENNFPELAYKTVESILLEDSIDVNPGICLDLTYRDKSSKVYEI"
     gene            2179..3222
                     /locus_tag="PKH42_05875"
     CDS             2179..3222
                     /locus_tag="PKH42_05875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOA19994.1"
                     /translation="MIIKIKLKSLLIFFAIIFVVLAIYLFMNKTQSVSADEEKDFIKW
                     VDFRVSYEAMDKALRADINSLDEEVKLDWIEILAYLGTRYGGDFFSRYKAKDMDKLIN
                     RLKSGETIESMTKDLKHYNYYYEAYKAVLEGFVGPFEIQVKDEDTGEKVWVKKYGLKA
                     FSPIAGGYHYGHYDDFGNSRSYGFRRVHLGNDLVGSIGTPIIAVESGRIEALGWNQYG
                     GWRIGIRSLDNKRYYYYAHLRKDHPYVKTLKEGDIVLAGDVIGYLGMTGYSRKENVNN
                     IRTPHLHFGMQLVFDESQKEALAEIWIDVYRIVKLLNRNRSPVVYNKETKEFERIYDI
                     RFHNIERKLSSIE"
     gene            complement(3371..4789)
                     /locus_tag="PKH42_05880"
     CDS             complement(3371..4789)
                     /locus_tag="PKH42_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19995.1"
                     /translation="MKTITRKIIMIFILLLIFNTTVYAGVNPSREEIEAIIDEVATKR
                     GIPAIILKGIARVESVFKHFNSDGSPKICGTSIGLMQINNVNGGYDNYRLKYDLLYNI
                     EAGADMLLNKWSMSSYKQVSSVGNMDPNILENWYFALWAYNGWSQSNNPVSPYAKKYT
                     YQQLVYDVIEKEYGKKINNIDFSYLPRESKPSRSLVVPTPNNYSSGNIIFYEKGDYVR
                     TDGIRKAYYLRDAPAGNYIYQLNVNQLGIITEGPLLKNGYYWYKVYIDETKEGWIERN
                     WLLRTGDTQYGRYIFEDIPFHWARKIIMDMYGRGIVSKAALYNPNNPVTYEEFCIFLS
                     RTLNYLENVNKQSKIQTSNKILYYDFTNVDLWALDFVKDIYERGLLKDIQPLQRLSRK
                     TAPLLIEDILTDSDKYLDIDINMVFSDLGNLNEEEISAVKKAYVNGIISGKDIKAFCP
                     DEYLTRAEATVIMSNIIKKYIR"
     gene            complement(4991..6499)
                     /locus_tag="PKH42_05885"
     CDS             complement(4991..6499)
                     /locus_tag="PKH42_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA19996.1"
                     /translation="MKILKTTARKVIIIIFVFVFTINTFVFAGVNPSREELELMIERV
                     AEKRAIPAILLKAIARVESCYEHYRADGSPKINGTSIGLMQINNKNGGYDSERLKYDI
                     MYNIEAGADVLLNKWAMSSYNEVASVGDMDPNVLENWYFALWAYNGWSQSNNPNIYQS
                     YTKKYTYQQLIYDVIEKEYGGKIHNIDFSYLPATGKPSRSLVVPTPMYTNGGNIIFYE
                     KGDYVRTDGIRTKFHLRDAPAGRYIHDLSLNQLGIIEDGPVLKNGYYWYKVYIDDNTE
                     GWIERNFLLRTGDAEYGRYVFEDISFHWARKIIMKLYGKNIVGEAEYFNPDKYVSKEE
                     FCILLNRALDYANVLNDSLGDDESKDDKDKEKSSRETETTSEEDASAILENIENINPW
                     AVEYVENVYESGLIDDEDLVNLLDNLNRKEAALIIANLFEISEEFSSLDIETVFTDIG
                     NLNEDEILAIKTVYTNGIMNGKGSGIFSPDANLTRAEAAVVMDKISERLSLQ"
     gene            complement(6530..8242)
                     /locus_tag="PKH42_05890"
     CDS             complement(6530..8242)
                     /locus_tag="PKH42_05890"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOA19997.1"
                     /translation="MYKEKFEMWLNSPHVDDVLKKELKEFEKIEIEIEDRFYRDLEFG
                     TGGMRGKIGAGTNRINLVTVSKATQGIANYLNEKYKGERISAAVAYDSRNMSKEFAEK
                     TACVFAANNIKVYIFDSLRPTPELSYAVRYFNCKAGVVITASHNPPEYNGYKVYDEFG
                     GQVVQDADKIIEKINEVKLEHIKSEEFIGNTKIQIVGNDVDTSYINDIKKLSLRNDVY
                     KNIKIIYTPLHGTGNIPVRRILDEMGYKNVFVVEEQEKPDSQFSTVSSPNPEELSSFE
                     IAIKKAKEMGGEIIIGTDPDCDRVGAIVKHKGEFIPLNGNQTGALLLNYLLASLDEQG
                     KIPDSPAVVKTVVTSEIANCIADKYNVTTFDTLTGFKYIAELMQKFEETKEYNFLFGY
                     EESFGYLAGTFVRDKDAVIASMLICEMAAYYKKQGKTLIDVLNEIYDEHGYYIEETVS
                     LNFSGVTGQSKMKAIMEHFRENKLSSLAGKKIPTIIDYKLSISLNLIKGTAERINYPK
                     SDVLKFSFEDGSWLVLRPSGTEPKLKVYFSINGHSRDESIESLEKIKSEILNIIDRIQ
                     INIG"
     gene            complement(8255..9121)
                     /gene="galU"
                     /locus_tag="PKH42_05895"
     CDS             complement(8255..9121)
                     /gene="galU"
                     /locus_tag="PKH42_05895"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOA19998.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAIPKEMLPIVDKPTIQYIVEEV
                     VASGIEDVLIITGRNKSSIEEHFDRAAELEYYLEKNNKEELLNEIKSISKMINIHTVR
                     QKDPLGLGHAIYCAKSFVGDEPFAVLLGDDVVDSQKPCLKQMLEVFEEYRSTILGVQP
                     VEWEKVHKYGIVSGSKINDRIYAVNGLVEKPGIENAPTNIAILGRYIITPKIFEILEN
                     TKAGVGGEIQLTDGLKELCNTEEIYAYIFQGRRYDVGSKIGFLEATVDYALKSDDLKE
                     GFKKYLRSINID"
     gene            complement(9139..10170)
                     /locus_tag="PKH42_05900"
     CDS             complement(9139..10170)
                     /locus_tag="PKH42_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOA19999.1"
                     /translation="MGLRMDIGIDLGTASVLVYIKGKGIVINEPSVVAIDINTNKILE
                     VGEEARKMLGRTPGNIVAIRPLRDGVISDFDITEKMLKYFIKKAVGNSFIKPRVIICV
                     PSGVTEVEKRAVLEASNNAGAKKTYLIEEPVAAAIGAGLDITKPSGHMVIDMGGGTTD
                     IAVISLGGIVVSRSIKVAGDKCDDAIVRYVRKKYNVMIGLRSAEELKIKIGTVFPVEE
                     ERFMEVRGRNLVTGLPVNLTISSSDSMEAMEETITTIADAVHSVLEKTPPELAADISE
                     KGIVMTGGGCLIHGMDKLLEKRTGLKVTIAEDAVSCVAKGTGQSLEHMDVLKDALITE
                     NIKMNYSIQ"
     gene            complement(10361..11515)
                     /locus_tag="PKH42_05905"
     CDS             complement(10361..11515)
                     /locus_tag="PKH42_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOA20000.1"
                     /translation="MNINEYTFEEPMPFGRLSIIAMKGCEEIAARIDYYLKEWRKDAN
                     GIIYSNGSGPIRTFLLDAACPRFGSGEAKGVINETVRGHDIFIISDVFNYGVKFKMYG
                     MDVPMSPDDHYQDLKRIIAAMNGKAKRITVIMPMLYEGRQHKRATRESLDCALALQEL
                     TSMGVENIISFDIHDPRVQNAIPLHGFEDFHPHYQMIKAFVRTVPDAKIDRDSMMIIS
                     PDEGGMTRCIYYSSVMELDLGMFYKRRDYSVIVNGKNPVVSHEFLGGNLEGKDVIVVD
                     DMIASGDSMIDVAGKLKERKVKNVYIFASFGLFTEGFNKFDKAYRDGIITRVFTTNMI
                     YRRPELLEKPWYTEVDMSKYIANIIDTLNYDGSLSELLDPVQKIKKLLKK"
     gene            complement(11713..12888)
                     /locus_tag="PKH42_05910"
     CDS             complement(11713..12888)
                     /locus_tag="PKH42_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA20001.1"
                     /translation="MKYNDLNEDIGREELAIIAERQNEKMAALGRLSGGIAHDYNNYL
                     MSIIGNATMIQKTDELDRIYDYAERIIHISQNAAELTKKILVFSKRKSSTNKPVNLKN
                     ILDNTYEMMEFILCKEIKLIYSYDANDEFILGDESQLESMIVNLILNSKDAIINNGII
                     KIGTKDTVVYSEMALSHGEILPPGKYIQIYIEDNGAGIDDEIMFKIFEPYFTTKIKTN
                     GTGLGLSVVFGTVKSHNGFLNVTSKTNNGTRFEIFIPVRKKSHDLGDKKIPEKENNIV
                     MLVDDDINVLEIEAEMLEDLGYDVVKFNAPLEALKYYENEYKNISFSVVDIMMPGLNG
                     KELYEKMKIINEEAVVIFITGFVQQAEYEDLMMRGLTVIEKPFTYEVLSKTIAEMYL"
     gene            complement(13053..13128)
                     /locus_tag="PKH42_05915"
     tRNA            complement(13053..13128)
                     /locus_tag="PKH42_05915"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13093..13095),aa:Lys,seq:ttt)
BASE COUNT         3758 a         2658 c         1904 g         4856 t
ORIGIN      
        1 tgcttagcgc aagctccggt aattcttctc ggtaatattt tgcctctttc agttgtatat
       61 ttttttaact ttccaaggtc tttataatct atgtgtgttg ccttttcctg gcagaattgg
      121 catacctttt tacggggttt tcttctttgg ctgtattttt catttttatc gtttatatct
      181 ttcccgcttt gtctggaact cattgttctc ctccttccgc aaatatatta aaatggaata
      241 tcatcgtcat ctacaggctc aaatatgtca ttgtcttcat cagggaaatc attgaaaaaa
      301 ctgctatccg gttgtactgc atttcccatg ggtgaggaag attgctcctt tgagccgata
      361 aattctactc tgtccgctat gacgtctgta gtgtatcttt tttctcctgt ttgtgtagta
      421 tagctacctg tttgaattct gccttgtatt gcgcactgtc ttcctttagt aaggtaatta
      481 gcgcataatt ctgcagtttt tccaaatacg gttatattta tgaaatctgc tgtctgtttt
      541 ccctgattta ttgcttcttg ttttttttct ccatataaac ctctatctac tgccaatgga
      601 aatttagcga cagccatacc cgttgagggt ataaatctta ggtcagggtc tcttgccagt
      661 cttcctatta atacaacatt attcataata ctaccccttt aattattttt cttctactac
      721 tgtaatcata tgtctgataa aattgtcgct tattttacaa attctgtcta cttcatcaac
      781 agcatctgct cctgcagtaa attcaataaa aatataataa ccttctcttt ttttgttgat
      841 ttcataagca agtcttcttt gaccccactc attaaccttt tcaattgttc cctgtctctc
      901 ggttattatg ctttgaattt tttcaatttc tgcatttctt acatcttcct ctaagttaga
      961 taataagata aaagcacctt catattttct catttgagtc acctcctttg gacttttggc
     1021 ccttgttcgc caagggcagg atagcttata cattatagca cttgaaaaaa cctaattcaa
     1081 gtattatttt attatgtatt cggcattttt tttacaatag ctgcattttt cttttttatc
     1141 tgtcttaatc aattgaggag cagcttcctt agaattcaca taatcatcta ttgcctgctc
     1201 cacatgctcc atgcatgcaa atatctcttc atacatttct ttcatgattc acctatattt
     1261 catatacttt gcttgattta tcccggtatg ttaaatcaag gcagataccc ggatttacat
     1321 cgatggaatc ttccaataat attgattcta ccgtcttata ggcaagctcg ggaaagttat
     1381 tttccttgct cagatgtcca agcaagatta tttctgttcc tttttttata acctttttta
     1441 ttaagttgcc tgctgcttca tttgacatat gtccgaattc acccaacact cttcttttaa
     1501 gcggccatgg atatggacct atgattacta agtcttcatc atgatttgat tccaccagca
     1561 ataaatctga atccataatg ctgtttgtaa ttgtaccatt gaaacaacct gtatctgtaa
     1621 ctaagcttat ttttttattt tcataaaata tattgaaacc taccggatgt gctgcatcgt
     1681 gggatatgtc aaatgacttg attgttaaat cccctatctg tgtgctttca tccaaaattt
     1741 taatattgca tgattttata tcaccaatta atgatttcat tgcttcccat gtttcttcat
     1801 tggcataaat gggaatattt aatcgccttg acattattcc tgcaccttga atatggtcaa
     1861 tatgttcatg cgttatgaat atggcatcta tcttattcgg atcttcccct atatttgcaa
     1921 tttcctgctg aattcttttt ccgcttaatc ctgcatcaac aataatagtt gtattttttg
     1981 tcttgatata ttggcagttt ccactgcttc cgctatatag cgaacagaat ttcagtttca
     2041 ttcgtcctcc cagtgtacat gaactacaat caatcaattt atactatcat aaaagcatta
     2101 gttttacaaa tactttataa ggttctatcc actcattatt tgcataatat ttactatatt
     2161 ataatatttg aggtaattat gattattaaa attaaattga aatcattatt gatatttttt
     2221 gctattatat ttgttgtatt ggcaatttat ttatttatga ataagactca atctgtttct
     2281 gcagatgaag aaaaggattt tataaagtgg gtagatttca gggtttccta tgaagccatg
     2341 gataaagctt taagggctga tataaacagt cttgatgaag aagttaaatt ggattggatt
     2401 gaaatactag cctatcttgg aacaagatat ggaggtgatt ttttttctcg atataaagca
     2461 aaagatatgg ataaattaat aaacagacta aaaagcggtg aaacaattga aagtatgacc
     2521 aaggatctca aacattacaa ttactactat gaggcataca aggctgtgct ggaaggattt
     2581 gtaggacctt ttgaaataca ggtaaaagat gaagacaccg gagaaaaggt ttgggttaaa
     2641 aaatacggat taaaagcatt ttcaccaata gcaggaggat accattacgg acattatgat
     2701 gatttcggga actcccgcag ctacggtttc cgaagagttc atttgggaaa tgacttggtt
     2761 ggttcgatag gtactcccat tatcgcagtt gaatcaggac gaattgaagc cttaggctgg
     2821 aatcaatacg gaggatggcg aataggaata cgcagcctgg ataacaaaag gtattactat
     2881 tatgctcact tgcgtaagga tcacccttat gtcaagaccc ttaaggaagg agatatcgta
     2941 ttggcaggag atgtaatcgg atatttaggg atgaccggtt attcgcgaaa agaaaatgtc
     3001 aacaacataa ggactcctca tcttcatttt ggaatgcagt tagtttttga tgagtctcaa
     3061 aaggaagcat tggcagaaat atggattgac gtatacagaa ttgtaaagct tctgaacaga
     3121 aacagatccc ctgttgttta taataaagag acaaaggaat ttgaaagaat ttatgatata
     3181 aggttccaca atattgaaag aaaactatca tccatagagt agcgaaaatc atataatatg
     3241 gggacacacc ttgagatgta ccttttgcgg gtcatacttt caaggtgtgt ccccaaatat
     3301 atgagattct tcgagctaat gctctcagaa tgaaaaaaat gttattcata gcaatggaga
     3361 agctgcagta ttaacggata tattttttta ttatgttaga cattattact gtggcttctg
     3421 ccctggttaa atattcatcc gggcaaaagg ctttaatgtc tttaccggat attattccgt
     3481 taacgtaggc ttttttaact gcagaaatct cttcttcatt aagatttccc aagtcggaaa
     3541 ataccatatt tatatcaata tctaaatatt tatcgctatc tgtcaatatg tcttcaatta
     3601 ataaaggagc tgtttttcta ctgagtcttt gtaagggttg tatatctttc aataaacccc
     3661 gctcgtaaat gtcttttaca aaatccaatg cccataagtc tacattcgta aaatcataat
     3721 agagaatttt gtttgatgtt tgtattttgc tttgcttgtt gacattttcc aaataattta
     3781 aagtcctgct caaaaatata caaaattctt cataggttac aggattattc gggttatata
     3841 gtgccgcttt gcttactatt ccccttccat acatatccat aataattttt cttgcccagt
     3901 ggaatggtat atcctcaaat atatatctgc cgtattgtgt atcccctgta cgcagaagcc
     3961 aatttctttc aatccagcct tcctttgttt catctatata tactttatac cagtagtatc
     4021 cgttctttag taggggacct tcggtgatta ttcccaactg gttaacattt agctgatata
     4081 tataatttcc tgcaggagca tcccttaaat aataagcctt cctgatgccg tctgtcctta
     4141 cgtaatctcc tttttcataa aatataatat taccgcttga atagttatta ggtgtcggga
     4201 ctaccaatga cctgcttggc ttactttccc tcggaagata agaaaaatca atattattta
     4261 tttttttacc gtattctttt tctataacat cgtaaacaag ctgctgatat gtatatttct
     4321 tggcgtaagg tgaaacggga ttgttgcttt gtgaccaacc gttgtatgcc cataatgcaa
     4381 aataccagtt ttccaaaata ttggggtcca tatttcctac acttgacact tgtttgtatg
     4441 aactcatgct ccatttgtta agaagcatat ctgcacctgc ttctatattg tacaataaat
     4501 catacttaag cctgtagttg tcatagcctc cgtttacatt gtttatctgc atgagcccaa
     4561 tgcttgttcc gcatatttta ggacttccgt cgctgttaaa atgcttaaac acggactcga
     4621 ctcttgctat tccttttaat attattgccg gtatacccct ttttgttgca acctcatcaa
     4681 taattgcttc gatttcttct ctagaagggt ttacacctgc atatactgtc gtgttaaata
     4741 tcaataaaag tataaaaatc attataatct tgcgggttat ggttttcaat atttacacct
     4801 ctctgttttt gtgaaattca caataatctt gtttataatg taaatattcc catattattg
     4861 aaaattatac cttttaggta tgcatttatt cgtaccaata taaaagtgaa ggggatagta
     4921 cctcttttaa gaagtactat ccccaatttg agattcttca ctacgttcag aatgacaatc
     4981 tacggttttt ttattgcaat gacaatcttt cggatatttt atccattaca acggcagcct
     5041 cagctctggt taaattggca tcaggactga atatccctga acctttaccg ttcataatgc
     5101 cgttggtgta gaccgttttt attgccagaa tttcatcttc attcaaattg cctatatcgg
     5161 taaatacagt ttctatatct aatgaagaaa actcttcgct gatttcgaat aaatttgcaa
     5221 taattaaagc tgcttctttt ctatttaaat tgtccaaaag attaaccaaa tcttcatcat
     5281 cgattaatcc cgactcataa acattttcta catattcaac agcccaagga ttaatatttt
     5341 caatattttc taatatagcg gatgcatctt cttcagatgt cgtttctgtt tcccgtgagc
     5401 ttttttcctt gtctttgtcg tctttagatt catcatctcc taagctgtca ttcagaacat
     5461 tagcatagtc caacgctctg tttaaaagaa tgcagaattc ttcttttgaa acatatttgt
     5521 caggattaaa atattctgct tcgccgacaa tattttttcc gtatagcttc attattattt
     5581 ttcttgccca gtgaaaggat atatcttcaa atacatatct gccatattca gcatctccgg
     5641 ttcgaagcag aaagtttctt tctatccagc cttcagtatt gtcatctata tataccttgt
     5701 accaataata tccgtttttt aacactggtc cgtcttctat aatacctaat tgatttaagg
     5761 ataagtcatg tatataccta cctgcaggag catccctcag atgaaatttt gtccttatac
     5821 cgtcagttct aacataatct cccttttcat aaaatattat gttaccgccg tttgtataca
     5881 taggtgtggg aacaaccaag gacctgctcg gtttccctgt tgcaggaaga tatgaaaaat
     5941 caatattgtg tattttcccg ccatattctt tttcaataac atcataaatc aactgctgat
     6001 aagtgtattt ttttgtatat gattgatata tatttggatt gttgctttgt gaccagccat
     6061 tgtaagccca taaagcgaaa taccagtttt ccaatacatt agggtccata tcgcctacac
     6121 ttgcaacttc attgtatgaa ctcatagccc atttattcag gagtacatca gctccggctt
     6181 caatattata cattatgtca tatttcaacc tttcgctgtc ataacctcca tttttgttgt
     6241 taatctgcat aaggcctatg cttgtaccat ttattttcgg actgccgtct gctctgtaat
     6301 gctcataaca agattcaact cttgctattg cttttaaaag aattgccggg attgctctct
     6361 tttctgcaac tctctcaatc attaactcca gttcttccct ggatggattt actcccgcaa
     6421 atacaaatgt atttatcgta aatacgaaaa cgaaaattat tataataact tttcgggcag
     6481 tggttttcaa tattttcata tttacagcct ccctatttag attttagtct tatccgatat
     6541 ttatttgaat gcggtcaatt atatttaata tctcgctttt tattttttct aagctttcaa
     6601 ttgattcatc ccggctatgt ccgttaatag aaaaatacac ctttaattta ggctctgttc
     6661 ctgagggtct caatacaagc caagagccgt cttcaaaaga aaacttcagg acatccgatt
     6721 tcggataatt tattctttca gcagtacctt ttataaggtt taatgaaata ctcaacttat
     6781 agtcaataat tgtcggaatc tttttacccg ctaaggaact cagtttgttt tctctgaaat
     6841 gctccataat tgctttcatt ttgctttgtc cggtaacacc ggaaaaattc agtgaaacag
     6901 tttcttctat atagtaaccg tgttcatcgt atatttcatt taatacatct attaatgtct
     6961 taccttgttt cttataatat gcagccattt cacaaatgag catagaagca atcactgcat
     7021 ccttatctct tacaaatgtt cctgccaaat agccgaaact ttcttcatat ccaaacaaaa
     7081 aattatattc ttttgtttct tcaaacttct gcatcaattc ggcaatatat ttaaaaccgg
     7141 tcaatgtgtc aaaagttgtt acattatatt tatctgcaat gcagtttgca atttcgctgg
     7201 ttaccacagt tttaacaact gccggactat caggaatttt cccttgctcg tccaatgaag
     7261 caagcaagta atttaaaagc aatgctccgg tttgatttcc gtttaaagga ataaattctc
     7321 ccttatgctt tactattgct cctaccctat cacagtcagg gtcagtacct ataattattt
     7381 caccgcccat ttctttagct ttttttatag ctatttcaaa agagctcagt tcttcagggt
     7441 ttggtgaact aaccgttgaa aattgagaat ccggtttttc ctgctcctca acaacaaata
     7501 catttttgta tcccatttca tccagaattc ttctcaccgg tatatttcct gtaccgtgaa
     7561 ggggagtgta tatgattttt atatttttat aaacatcatt tcttaagctt agtttcttta
     7621 tatcatttat ataagatgta tcaacatcat ttcctactat ttgaatcttt gtatttccaa
     7681 taaattcttc tgattttata tgctctaatt ttacttcatt aattttctca ataattttat
     7741 cagcatcttg aaccacctgt cctccaaatt cgtcgtatac tttgtaaccg ttatattccg
     7801 gaggattatg gcttgcagtt attactacgc ctgccttgca attaaaatac ctcacagcat
     7861 aagataattc aggagttggt ctaaggctgt caaaaatata aacttttatg ttatttgcag
     7921 caaatacaca tgctgttttc tctgcaaact cctttgacat atttcttgag tcatatgcaa
     7981 cagctgctga aatcctttca cctttgtatt tctcattcaa ataatttgca attccctgag
     8041 ttgctttaga aacggtaacc aaatttatgc gattagttcc tgcaccaatc tttcctctca
     8101 tacctccggt accgaattcc aagtccctat aaaatctgtc ttctatttct atttcaattt
     8161 tttcaaattc ttttaattct ttttttagca catcgtcaac atgtggactg ttaagccaca
     8221 tttcaaattt ttctttatac atttccccca ccccttaatc tatgtttatt gaccttaaat
     8281 acttcttaaa gccttctttc aagtcatcac ttttgagagc ataatcaaca gttgcttcaa
     8341 gaaagccgat tttactgcct acatcatatc ttctgccctg gaatatataa gcatatattt
     8401 cttcggtatt acataattcc tttaacccgt ctgtaagctg aatttctccc cccacaccgg
     8461 ctttcgtatt ttcaagtatt tcaaatattt tcggagttat tatatacctt cctaatattg
     8521 caatatttgt aggtgcattt tctataccgg gtttttcaac caaaccattc acggcataaa
     8581 tcctgtcatt gattttactg cctgacacta tgccgtattt atggactttt tcccactcta
     8641 ccggttgcac accaagtatt gtagagcgat attcctcaaa aacttccaac atttgcttta
     8701 ggcacggctt ttgtgaatca acaacatcgt cacctaagag cactgcaaag ggctcatcgc
     8761 caacgaagga tttcgcacag taaattgcat gacctaaccc caaagggtct ttttgcctta
     8821 ctgtgtgaat atttatcatt ttggaaatac ttttaatttc atttaaaagc tcttccttat
     8881 tatttttctc taagtagtat tcaagctctg ctgccctgtc aaaatgctct tcaattgaac
     8941 tcttgtttct tcctgttatt attaatacat cttcaattcc ggaagcaaca acttcttcta
     9001 ctatgtattg tattgtcggt ttatccacaa tgggcagcat ttccttagga attgctttcg
     9061 ttgcaggcaa aaatctagtg cccaaccctg ctgcaggtat tactgccttt ctaactttca
     9121 tgtttacctc tgcttcagct attgaatgct gtaattcatt tttatatttt cagtaatcaa
     9181 tgcatctttt agaacatcca tatgctccaa agactgtcct gttcctttgg ctacacaaga
     9241 tacggcatcc tctgctatgg ttacttttaa tccggttctc ttttcaagca gtttatccat
     9301 accatgaatg aggcatcctc cgcctgtcat tacaataccc ttttcgctga tatcagctgc
     9361 aagctccggc ggagtttttt caagaactga atgtaccgca tcagcaattg ttgtaattgt
     9421 ttcttccata gcctccatag aatctgaaga tgaaatagtc aaattaaccg gaagaccggt
     9481 aaccaaattc ctgcctctca cttccataaa tctttcttct tctacaggaa ataccgttcc
     9541 tatttttatt ttaagctctt ctgcacttct tagacctatc atgacattgt atttctttcg
     9601 tacatatctt acaatcgcat cgtcacactt atcaccggca accttgatag acctgcttac
     9661 aactatgcct ccaagagata taacagcaat atccgttgta cctccgccca tgtctataac
     9721 catgtgcccg gatggctttg ttatgtctag acctgctcct atggcagctg caacaggctc
     9781 ttcaataaga tatgtctttt ttgcacctgc gttattactt gcttcaagaa ctgctctttt
     9841 ttcaacttct gtaacaccgc ttggaacaca aattataact cgcggtttta taaatgaatt
     9901 tcctactgct ttttttataa aatatttcaa cattttttcc gtaatatcga aatcagatat
     9961 aacaccatct cttaagggtc taattgctac aatattgccc ggagtacgtc caagcatttt
    10021 tcttgcttct tctcccacct caaggatttt gttagtatta atatcaatag ccacaacaga
    10081 aggttcattt ataactattc ccttaccctt aatataaacc aatacactgg cagtgcccag
    10141 gtcaataccg atatccattc tcaggcccat cttcatcctc cacgtttctt cttattatct
    10201 tatctttaaa atattaatga taaagattat aacatacaac ttaatttatt tcaatttatt
    10261 ttatcccgga aatcgcacag gaatacacaa actaaaataa attaataaat aagagttatg
    10321 ccctacaata aaaatgcctg caaagcagac attgtatatt ctatttttta agcaattttt
    10381 tgattttttg aaccggatcc agtaattcgc ttaaggaccc gtcataattt aatgtgtcta
    10441 ttatgtttgc aatatatttt gacatatcca cttcagtgta ccaaggcttc tctaacagtt
    10501 ccggtcttcg ataaatcatg tttgtagtaa aaactctagt tattatacca tctcggtatg
    10561 ccttgtcaaa tttattaaat ccttcggtaa acaatccaaa ggaagcaaat atatatacat
    10621 tctttacctt tctttccttt aatttgccgg caacatcaat cattgaatca ccggaagcaa
    10681 tcatgtcatc aacaacaata acatcttttc cttccaaatt accgcccaag aattcatgac
    10741 tgacaacagg attcttgcca ttaactataa cagaataatc tctcctctta tagaacattc
    10801 ctaaatctaa ttccataacc gatgaataat aaatacatct tgtcattccg ccttcatcgg
    10861 gagatattat catcatgctg tctctgtcaa ttttagcatc aggcactgtt cttacaaatg
    10921 cttttatcat ttgataatgc gggtgaaaat cttcaaatcc atgaagaggt atagcatttt
    10981 gaactctcgg gtcatgtata tcaaaagaaa taatattttc aactcccatt gatgtaagct
    11041 cttgtaatgc caaagcacaa tccaaagatt ctcttgtggc cctcttatgc tgtctgcctt
    11101 cataaagcat tggcattata actgttatgc gctttgcctt accattcatt gcagcaatta
    11161 ttcttttcaa atcctgatag tggtcatcag ggctcatggg cacatccatg ccgtacattt
    11221 tgaatttaac tccgtaatta aatacatcag atattataaa tatatcatga cctcttactg
    11281 tttcgtttat tactcccttt gcttcaccgg agccgaatct tggacaagcg gcatctaaaa
    11341 gaaatgttcg tataggacct gaaccatttg aatatataat accgttcgca tccttcctcc
    11401 attcctttaa ataatagtct attcttgcag ctatttcttc gcatcctttc attgcaataa
    11461 tagataatct tccaaatggc ataggttctt caaacgtgta ctcatttatg ttcatttctt
    11521 cctcctgttt accttatcat gttacatttt atcaaaaaaa tatcaattgt caaaagaata
    11581 tgattaccat attattaaat tatcttaaca atgtctttgt aaacattgaa attgaagaaa
    11641 agctcaattt tcataataaa ataaccgcct ttgcttcgcg gttatttaaa ggtggaatat
    11701 tcttggatat agttataaat acatttctgc aattgtcttt gacagtacct catatgtaaa
    11761 cggcttttca attacggtaa gtcctctcat cattaaatct tcatattctg cctgctgtac
    11821 aaaaccggtt ataaaaatca ccactgcttc ttcatttatt attttcattt tttcataaag
    11881 ttctttgccg tttaatccag gcatcattat atccactaca gaaaaagata tatttttata
    11941 ttcattttca taatatttta atgcttcaag aggagcattg aattttacta catcatagcc
    12001 taaatcttcc aacatttctg cttcaatttc caaaacattt atgtcatcat caacaagcat
    12061 tacaatattg ttttcctttt ccggtatttt cttatcgccc aaatcatgac tctttttgcg
    12121 tacaggtata aatatttcaa accttgtccc gttattagtt ttgcttgtaa catttaggaa
    12181 accgttgtgg gatttcaccg taccgaacac tacagacaag cctagacctg tcccgtttgt
    12241 tttaattttt gttgtaaagt atggctcgaa tattttaaac attatttcat catctatacc
    12301 tgcaccgttg tcctctatat aaatttgaat atattttccg ggtggcagta tttcgccatg
    12361 acttaatgcc atttccgaat aaactacggt atccttggtt cctattttta ttattccgtt
    12421 attgataatt gcatcctttg aatttaatat caggtttact atcatacttt caagctggga
    12481 ttcatccccc aaaataaatt catcattagc atcgtaacta tatatcaatt tgatttcttt
    12541 acagagaata aattccatca tttcataagt attatccaat atatttttta aatttacagg
    12601 tttatttgta ctgctttttc tctttgaaaa tacaagaatt ttttttgtaa gctccgctgc
    12661 gttttgtgag atatgaatta ttctttcagc ataatcatat attctgtcaa gctcatcggt
    12721 tttttgaatc atggtggcat tacctattat actcataagg taattattgt agtcatgagc
    12781 tattcctccc gaaagccgac ccaaggcggc catcttttca ttttgtcttt cagctataat
    12841 tgccaattct tctctgccga tatcttcgtt caaatcatta tatttcatcg caacctcttt
    12901 acaaatttat ttgtatttaa tatacacttt ttggattaaa tattttgtag aaaattgttg
    12961 aaaaggtaaa aatttgcaga tataagtagt cctctttcct taaataaaaa gcaacattca
    13021 acgtcgcgaa gatcgtcaaa tgttgtttat aatggtgatc catcggcgat tcgaacgccg
    13081 gacaccttga ttaaaagtca agtgctctgc ctgctgagct aatggatcaa tacatattaa
    13141 cttttatata atacattcct tgtcgtatct tgtcaa
//
LOCUS       DAOPUE010000050        13131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4833_length_13131_cov_9.803839, whole genome shotgun sequence.
ACCESSION   DAOPUE010000050 DAOPUE010000000
VERSION     DAOPUE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_13131_cov_9.803839"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      95..177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            233..2458
                     /gene="pflB"
                     /locus_tag="PKH42_05920"
     CDS             233..2458
                     /gene="pflB"
                     /locus_tag="PKH42_05920"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOA20002.1"
                     /translation="MKEWNNFIQGRWTKEIDLRDFIQKNYSPYEGNEEFLSGATEKTK
                     NLLKKVSRLMTEELKKDVLDVDTDNISGINNFEPGYLDKDNEIIVGFQTDAPLKRMIN
                     PYGGIRMVEQSLEAYGYSMSDELCNVFHKYRKTHNEGVFDVYTPEILLARSSGLLTGL
                     PDAYGRGRIIGDYRRIALYGIDFLVEKKKEEYNNIKFPMNEDSIRAREEIKEQIKALF
                     DMKSMASKYGIDISKPSSNALEAVQAVYFGYLAGVKENNGAAMSLGRTSTFIDIYIER
                     DIKEGILSEKEAQEIIDQFVLKLRMVRHLRTPEYNELFAGDPNWVTEALGGMGLDGRT
                     LVTKTSFRFLHTLTNLSAAPEPNMTVLWSERLPEGFKKYCTKMSIDTSAVQYENDDIM
                     RPIYGDDYGIACCVSAMAIGKEMQFFGARCNLAKALLLGINGGTDEISKETVITNINK
                     INSKILNYDEVLNNYKKVLEYVAELYVNTMNIIHYMHDKYAYESGLMALHNYDVRRLM
                     AFGVAGLSIAADSLSAIKYAKVKPVYGEDGITEDFEIEGEYPMYGNDDDRVDGIAAEL
                     LEFFSNELKKHKTYRNAKHTLSVLTITSNVVYGKKTGATPDGRKKGEPFAPGANPMNG
                     RDKSGLLASLNSVAKLKYKDICEDGISNTFTVVPDTLGKKYRHRIDNLSNILDGYFSQ
                     GAHHINVNVINRDTLYEAMEAPHKYPNLTIRVSGYAVNFNRLTREQQMDVIGRTFHDR
                     M"
     gene            2461..3228
                     /gene="pflA"
                     /locus_tag="PKH42_05925"
     CDS             2461..3228
                     /gene="pflA"
                     /locus_tag="PKH42_05925"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOA20003.1"
                     /translation="MTAGFLHSIETLGTLDGPGLRMIVFLQGCKLRCLYCHNPDTWIE
                     NITPGDNKNLTDKYYLKERFTADYIAEKAKRYKPYFKNNGGITFSGGEPLRQPKFLLD
                     CLMLCKAEGIHTVIDTSGVGFGDYDEILKYTDLAILDIKHSNPEKYKKITGCDIEYYL
                     DFKEAVIRNKIQLWIKHVVTPGINDTEGDMKDFENEVKTFPDKLIQKIELLPYHTLGV
                     YKYKELNIKYKLEGVPPLSKDRLQELKDCITLEKLIR"
     gene            3285..3752
                     /locus_tag="PKH42_05930"
     CDS             3285..3752
                     /locus_tag="PKH42_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOA20004.1"
                     /translation="MSVEKVREYLKQFNKENDIKEMDESTATVELAALALDTEEARIA
                     KSLSFYNGDGAMVIVVAGDAKIDNRKFKDYFGFKARMLSPEDTKKFTGHEVGGVCPFA
                     LPDNVKVYLDVSMKRFETMFPACGSGNSAIELTLDELEETSKSNTWIDVCKNW"
     gene            3931..4683
                     /locus_tag="PKH42_05935"
     CDS             3931..4683
                     /locus_tag="PKH42_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOA20005.1"
                     /translation="MNEFSRTQLLLGKQAMEKLSSSTVAVFGIGGVGTFVVEALARCG
                     VGNFALFDDDKVCITNINRQLIATRKTIGRKKVEVMAERIKEINPKASTATYQVFYMP
                     DNADEYDLTSYDYIVDAVDTVTAKIELIVRAKKNNIPIISSMGAGNKLNPTAFEVADI
                     YKTSVDPLAKVLRHELKRRGIENLKVVYSKEKPLTPSCDEDSCKDSCVCPPGTTRKCT
                     QRRAIPGSVSFVPSAAGLIIASEVVKDIIAGI"
     gene            4899..5780
                     /locus_tag="PKH42_05940"
     CDS             4899..5780
                     /locus_tag="PKH42_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOA20006.1"
                     /translation="MEVGSRSMVDKIQTIVIKHAREAYVSQEYLNENWQKWGFFSCPD
                     YNKCVEEYDAFEALIKKLVPDVRLLPKDEESGLDSIYTHDSVKFTEKGAIILNMGKNA
                     RKNEPKAMKVYLESIGVPILGEITGEGTVEGGDVLWLEDNRVAIGRGYRTNDEGIRQF
                     KELVKDLVDEVIVVPMPHGNNEEECLHLMSVISPIDKDLAVVYSRYMPVFFRQMLIKK
                     GIKLIEVIDDEYMPLGCNVLTIEPRKVVLLKGNPDIKKKLLNEGVEVHEYEGQHISLP
                     GNGGATCMTAPLLRIKN"
     gene            complement(5847..6503)
                     /locus_tag="PKH42_05945"
     CDS             complement(5847..6503)
                     /locus_tag="PKH42_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA20007.1"
                     /translation="MSQYLTLKDHVYNYISSKINDGSLKPQDKINEQQISDELEISRT
                     PIREALIQLASEGYLENRARKGFRVRSLDYEKTKELYEIIGLLDGRIAYMTVDLINDN
                     HLKNMRFLADSMASAIEQGLNQKYYELQIEFHNIYTDLYNNQEMINLLNKLKKNFMRK
                     YYFFETKTDEKAILKKTNDQHYEIIRLFEEKKKEELERYIRDIHWAVDIAIYDSLQNY
                     "
     gene            6792..7562
                     /locus_tag="PKH42_05950"
     CDS             6792..7562
                     /locus_tag="PKH42_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOA20008.1"
                     /translation="MFRNVIVRRPSKSMVEGITSSPELGKPDYELALKQHDAYIEALK
                     ACGVKVTVLEALEEYPDSCFVEDTAVLTPKCAIITNPGALSRRGESALMVDTIKKFYN
                     EDQIEYITSPGTIEGGDVMMVGNHFYVGLSARTNEEGFRQFKAALEKYGHTSSLVSLE
                     KVLHLKTGVNYIENNIMLISGEFIEKEEFKSFNKIIVPENEAYGANCIWVNDTVIVPE
                     GYPTVEKGVRDTGYKVIVVDTSEYRKLDGGLSCLSLRF"
     gene            7673..8839
                     /locus_tag="PKH42_05955"
     CDS             7673..8839
                     /locus_tag="PKH42_05955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA20009.1"
                     /translation="MKHKFIAKRYWNTKSSAMGEIGPLMSKYNDVIDLTFGDPDFTTD
                     EIIIKEAFKDALNGHTHYTSFYGDDELRDEICKYYHDEFNIKVERNETFISTSAAQGL
                     WLALETITDDGDEIIIHEPYFTPYPDQIKLTRGIPVFLETFEEEGFQINVERLEKLIT
                     NRTKAIIINSPNNPSGARFSDETLLKIADIAEKYDLIVIVDDIYGLYTFDSENRNTPF
                     MSIKNMKERTITICSASKDYAMTGWRIGYLIAPDYLVDAMEKVNENNVFTAPSVSQRA
                     MLHAIRNRKIIQPKLFNIFKERVFYTYDRICQTKNMSVLPPRGTFYLFPNIKKTGLSS
                     QEVSMKILDEAHVLTIPGSGFGSSGEGYIRIACTVGIDKLGEAFDRINKMDIFR"
     gene            8865..>9542
                     /locus_tag="PKH42_05960"
     CDS             8865..>9542
                     /locus_tag="PKH42_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOA20010.1"
                     /translation="MNICIIDAKQFITDEKLIEPLYKLGNVSMYDGIPQSLDETIKRA
                     KDADIVLFALMKFTNEIIDNLPNLKILQFIGTGVWNFVDALYAEKKGIKVLNIEGYGN
                     NAVAEFALSCAFSLARKITNADRILKERRWSLDNLEGIEIEGSVFGVVGTGNIGSIVA
                     KKACLLGADVIACDIYESDELKNKYGIKYVSLSEIFEKSDIITIHMKATKETEKIIGK
                     DLLSKMKK"
     assembly_gap    9543..9642
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9675..11090
                     /locus_tag="PKH42_05965"
     CDS             9675..11090
                     /locus_tag="PKH42_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOA20011.1"
                     /translation="MGPIDLIIFALYMIGMVVIGFIAQGKIETMDDFILGGRRFGLVA
                     LVGTIMATMIGSGMTMGAVGNAYKAGAGGTVFWMYTGFSIGLLYFGYIAGKIRETGKR
                     TVAEVLSAKFGNNTRLISSIVIIGYAVSIVAINIAGLRNVIFYVFGSKLGISLPLATT
                     IAAAICILYTSLGGFYAVVWTDVVQFGLMLLGIVILGPIIGLNSAGGFNNVVLEYEKI
                     GSSITNPFINGISSGSIGFFLAYFLTVPGDPTMPQRALAAKDEKSAKISFYISGVLGF
                     FFGFALLIMGGSAFVLMPGLENPESAMLLFISEYYPPILKGLSISAIVAAIMSSFDSF
                     LILATTHLVYDVGSSLNKDVNEEKMKKLLPKVTIVIGILGLIIALFIQSLFDYLYMVF
                     SIIGSSLVPAIIAALYYSDKTTSKAVVASIVTGAVVPGALYLTYGYDVFLGDPVFLGI
                     IASTLVLIIGSIVTKDKIATK"
     gene            complement(11257..13050)
                     /locus_tag="PKH42_05970"
     CDS             complement(11257..13050)
                     /locus_tag="PKH42_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOA20012.1"
                     /translation="MSRIKNFMNNIVYNLKETITRFPMTIVFLASLSTVMFLIIEDYS
                     SLDIDLLSRYMFAGIFGAFLATAVRFMLERYENLKNSFLFYGLTILLTIGYFYFMTDD
                     KVDNKMLIHLLVISFALFAAYLYLPSSKNALNFGNVALAHFKSAFTAILYGIVLYVGI
                     AAIIGAIDILLYDVDYKSYAHAANIIFIFFTPLYYLSLLPKFNSRDENDDAKKEISFT
                     YPRFLDILVSYITIPLITAFTAVLIIYFIKILATGVWPVGQVGPMVLGYSAAGYFIYI
                     LSSNLNNKFSVLYRKLFPLILIPLVIMQMVSSYIRIDAYGITESRYYVVLFGIFSIVC
                     ALMLIFDKKKNPNTIVLLSAVFALMSIIPPVDAFTVSKNSQERRLTEILNRNKMIVDG
                     EIVKKTDLSTDDMFEITNISNYMYGMGYLDDMDWFPDKYADNYYGNFKNIYGFEQYYD
                     RGFPGQEETLYLYAILDENEKINIEDYDLFFRIYIQNKRGPGEDIGEFSLKGKNYSIK
                     QIPDEKGYITITVSDDTNTLIEIPMKEFIDGLFEKSNAPKGAMDKESLTIEKQNDKLK
                     VKLLIYDMNVDRNDPDDIYISANIYVFVSTQ"
BASE COUNT         4623 a         1905 c         2521 g         3982 t
ORIGIN      
        1 tcttttcgga gatgcattga gagatacgct tgatccgaga ctgaaagatt aagagcataa
       61 agtattgaca aaaaatgtta tttattgtaa cataagagta tcacatgact ggcggaaaag
      121 tggagtacca cgaggaagtg tgaataaaat taaaagtcgg ccgcctgggc taatcccggg
      181 cggcttttta aatttcaaaa catagcctcc cggaaaaacc ataggaggaa ctatgaaaga
      241 gtggaacaat tttattcaag gaagatggac aaaagaaatt gacctaagag attttattca
      301 aaaaaattac agcccttacg aaggaaatga agaattctta agcggcgcaa cagaaaaaac
      361 caaaaactta ttaaaaaaag tatcaaggct tatgacagaa gaacttaaaa aagatgtttt
      421 ggatgtggat accgataata tttcgggaat taataatttt gagccaggat atctagacaa
      481 ggacaatgaa ataatcgtgg gatttcaaac agatgcaccc ctgaagagga tgattaatcc
      541 atacggcgga atcagaatgg tggagcagtc tttggaagcc tacggatact ctatgagcga
      601 tgagttatgt aatgtttttc ataaatacag aaaaactcac aacgaaggag tatttgatgt
      661 atacacacct gaaatactat tggccagaag ctccggactt ttgacaggtc ttcccgatgc
      721 atacgggaga ggaagaataa tcggcgacta tagaagaatt gctttgtacg gcatagattt
      781 tctggttgag aaaaagaagg aagaatataa taatattaaa ttccctatga atgaagatag
      841 catcagagca agggaagaaa ttaaagaaca gattaaagca ttatttgaca tgaaatcaat
      901 ggcatctaaa tatggtattg atatttcaaa accttcttca aatgcattgg aagctgtaca
      961 ggcagtctat ttcggatatt tggcaggggt gaaagaaaat aacggagctg caatgtcatt
     1021 aggcaggaca agcacattta tagacatata tatagaaagg gatataaaag aaggtatact
     1081 cagcgaaaaa gaagctcaag aaattattga ccagtttgtt ttaaaactta gaatggttcg
     1141 tcacttaaga actcccgaat ataatgagtt gtttgcaggg gacccgaact gggttaccga
     1201 ggcattggga ggaatgggac ttgatgggag aacattggta actaagacat ctttcaggtt
     1261 tttgcacaca ttaacaaatt tgtcggctgc accggaaccg aatatgacag ttttgtggtc
     1321 ggaaaggctg cctgaaggtt tcaaaaaata ttgcactaag atgtccattg atacatctgc
     1381 tgttcaatac gaaaacgatg acataatgag acctatttac ggtgatgatt acggtattgc
     1441 atgctgcgta tccgcaatgg caataggcaa agaaatgcaa tttttcgggg caagatgcaa
     1501 tttggcaaaa gcactgctct tagggattaa cggcggtaca gatgaaatca gtaaagaaac
     1561 agtaataaca aatataaata aaataaattc aaaaatactc aattatgacg aagtgctcaa
     1621 caactacaaa aaagtactgg aatatgtagc ggagctttat gttaatacta tgaacataat
     1681 tcattacatg catgataaat atgcttacga atcaggactt atggctcttc acaactatga
     1741 tgtgagaagg ttgatggcct ttggagttgc agggctttca attgctgcag actctctgag
     1801 tgccataaaa tatgcaaagg taaaacctgt ttacggcgaa gacggtataa ccgaggattt
     1861 tgaaatagag ggagaatatc caatgtacgg taatgacgat gaccgtgttg acggtattgc
     1921 tgcggaatta ttggaatttt tctctaatga attaaaaaaa cataaaacct acaggaatgc
     1981 aaagcataca ctttcagtat taactattac atcgaatgtt gtttacggca agaaaacagg
     2041 agcaacacct gacgggagaa aaaaaggaga gccctttgca ccgggagcaa atccaatgaa
     2101 cggaagggat aaaagcggat tgcttgcatc cttgaattcg gttgcaaaat taaagtataa
     2161 agatatttgc gaagatggaa tttcaaatac ttttacggta gttcccgata cattgggaaa
     2221 aaaatacaga cacagaatag ataatttgtc aaatatactg gacggatatt tttctcaggg
     2281 ggctcatcat ataaatgtta atgtaattaa cagggatact ctttatgaag ccatggaagc
     2341 tccccataaa tatccgaact taactataag agtttccggt tacgcagtaa actttaacag
     2401 actgactcgt gagcagcaaa tggatgtaat aggaagaaca ttccacgaca ggatgtaaaa
     2461 atgactgccg gatttcttca ttcaatagaa acattgggta ctttggacgg accggggctt
     2521 agaatgatag tgtttttaca ggggtgcaaa ttaagatgtc tttattgtca taacccggac
     2581 acatggatag aaaacatcac tccaggcgac aataaaaact tgacagataa atattatctc
     2641 aaagaaaggt ttactgcaga ttatattgct gaaaaagcga aaaggtataa accttatttt
     2701 aagaataacg ggggcataac tttttcagga ggtgagccat tgaggcagcc aaaatttttg
     2761 ctggattgcc ttatgctgtg caaagctgaa ggaatccata cggttataga tacatcggga
     2821 gtaggttttg gagattatga tgaaatatta aaatataccg atttggcaat actcgacata
     2881 aagcattcaa atcctgaaaa atataaaaag ataaccggct gcgatattga atattatctg
     2941 gactttaagg aagcggtaat cagaaataaa atacagcttt ggattaaaca tgttgtaacg
     3001 ccgggaatta atgatacaga aggagatatg aaagactttg agaatgaagt gaaaactttt
     3061 cctgataaat tgattcaaaa aatcgagctg ctgccttatc acacattggg agtgtataaa
     3121 tacaaggaac taaatataaa atataaattg gaaggggtcc ctcctctgtc aaaagacagg
     3181 cttcaagaac ttaaggattg tattactttg gaaaaactga taagataata aaagaagtat
     3241 ttgacatagc gatagatagc ataaagaagt attaggaggg aaaaatgtca gtagaaaagg
     3301 taagagagta tttaaaacaa tttaacaagg aaaatgacat aaaagaaatg gacgaatcaa
     3361 cagcgaccgt agagttggct gctctggcct tagatacgga ggaagcgaga attgcaaaat
     3421 cattatcatt ttataacggt gatggagcaa tggtaatagt agtagcagga gatgcaaaga
     3481 tagataatag aaaattcaaa gattatttcg ggtttaaagc aagaatgctt tcacctgaag
     3541 atacaaaaaa attcaccgga catgaagtcg gaggggtttg tccttttgca ctgcctgata
     3601 atgtaaaagt ttacttggat gtatcaatga aaagatttga aactatgttt cctgcctgtg
     3661 gaagcggtaa ctctgcaata gagctcaccc ttgatgaatt ggaggaaaca tcaaaaagca
     3721 acacctggat tgatgtatgt aaaaattggt gataaagagc aatcaaagat ttgtaacttt
     3781 tattaataat tgtaattttg agggcgcagc ccgtgtcatc ctgagcgtaa gcgaaggatc
     3841 tcagatttta aaagatgaga ttcttcacct agggttcaga atgacgctct atgggttact
     3901 cataacaatg acataaaaag aaaggataat atgaacgaat tttcaagaac acagctacta
     3961 ttaggtaaac aagcaatgga aaaattaagc tcttccacag ttgcagtatt cggcatcgga
     4021 ggagtaggca catttgttgt tgaagctttg gcaagatgcg gcgtaggaaa ttttgcactg
     4081 tttgatgacg acaaagtatg cataacaaac ataaacaggc agttaattgc aacaagaaaa
     4141 accattggca ggaagaaggt tgaagtgatg gcagaaagga ttaaggaaat aaatccaaaa
     4201 gccagtacag caacttacca ggttttttac atgcctgaca atgcggatga atatgattta
     4261 acttcttatg attatattgt agatgcagtt gatactgtta ccgctaaaat agaattgata
     4321 gtaagagcaa aaaaaaacaa tattcccatt atcagcagca tgggagcagg aaacaaatta
     4381 aatcccactg cctttgaagt tgccgatatt tacaaaacat cggtagaccc cttagcgaag
     4441 gtattgcgtc atgaattaaa aaggcggggt attgaaaatt taaaggtagt atattcaaaa
     4501 gaaaaacctc tgactccaag ctgcgatgaa gacagctgca aggacagctg cgtatgcccg
     4561 cccggaacca caagaaaatg cacccaaaga cgtgcaatac cgggaagtgt atcatttgta
     4621 ccttctgcag cagggctgat tattgcaagc gaggttgtta aagatattat agccggcata
     4681 taaaagttaa cgaataattt tcatatatat cctctacaaa tgttttatgc atttgtagag
     4741 tttttttgta tgagaaatta ataatcatcc ttaacgataa aaagaactac ttgatttaag
     4801 tataatttac ttgacaatgt ctactggtat tatttataat aaaaacaaaa ataaaatata
     4861 caaaatattg tatacaataa tgaaagagag gtctaaaaat ggaagttggt agtcgctcaa
     4921 tggttgacaa aatccaaact atagttatta agcatgcaag agaagcatat gtcagccagg
     4981 aatacttgaa tgagaattgg cagaagtggg gttttttttc ttgccctgat tacaacaaat
     5041 gtgtagaaga atacgatgca tttgaagctt taataaaaaa attggtaccg gatgtgagat
     5101 tattaccaaa ggatgaagaa tccggacttg attctattta tacacatgat agtgttaagt
     5161 ttaccgaaaa aggtgcgata attctaaata tgggcaagaa tgccagaaaa aacgaaccaa
     5221 aggctatgaa ggtttactta gagagtatag gagttccaat tttgggcgag ataaccggag
     5281 aaggaaccgt tgagggtggt gatgtactgt ggttagagga taacagagtt gcaatcggaa
     5341 ggggttaccg taccaacgat gagggaatca ggcagtttaa agaattggtt aaagacttgg
     5401 ttgatgaagt aatagtagtg ccgatgcctc atggcaataa tgaagaagaa tgtcttcatt
     5461 taatgtctgt tataagtccc atagacaaag atcttgcagt agtatactcc agatatatgc
     5521 ctgtgttttt tagacagatg ttaattaaga aaggtataaa attgattgaa gtaatagatg
     5581 acgaatatat gcctctagga tgtaatgtac ttactattga gcctagaaag gttgtattgc
     5641 tcaagggaaa tcccgacatt aagaaaaaac tgttaaatga gggcgtagaa gttcacgagt
     5701 atgaaggaca gcacatatct cttccgggaa atggcggagc aacttgtatg acagctcctt
     5761 tattaagaat aaagaattaa gagtatatga cccatagtat gactataata taaaagataa
     5821 cgacacttag tcagtgccgt tattttttaa taattttgta aagaatcata tatagctatg
     5881 tcgacagccc aatgaatatc cctgatatat cgttccaatt cttctttttt cttttcttca
     5941 aataacctga taatttcata gtgctggtcg tttgttttct ttagtatagc tttttcatca
     6001 gtttttgttt caaaaaaata atatttgcgc ataaaattct tttttagttt atttaataaa
     6061 ttaatcattt cttgattatt gtataaatca gtatatatat tatgaaattc tatttgaagt
     6121 tcataatatt tttggttaag accctgttcg attgccgatg ccatcgaatc tgccagaaat
     6181 cgcatatttt ttaagtggtt gtcattaatt aaatctaccg tcatgtaagc tattcttcca
     6241 tccaacaatc caattatttc atagagttct tttgttttct cataatctaa actccttacg
     6301 cgaaaacctt tacgggctct attttctaaa tatccttccg atgccaattg aatcagggct
     6361 tctctgattg gtgttctgct tatttcaagt tcatcggaaa tttgttgttc atttattttg
     6421 tcttgcggct ttaaagaacc gtcatttatc ttagatgata tataattgta tacatgatct
     6481 tttaaggtta aatattgcga catttttttc tccatatttt atttttttta aatattataa
     6541 cataaaatta ccaaaaaaca atatattata tacaaaataa ttgtacgata ttatacaaat
     6601 ttaataatat tatatttaat aataaaaatc attaattttt tatttttaat tacaaaaata
     6661 ttaccaaaaa aatacttgca tttatcctaa tttgatacta aaatataatg tatacaatat
     6721 aaagtatacg tgtactaaca gaaaaatatt aagaaatgaa tggtaaaatt aaaattatct
     6781 tggaggaaat tatgtttaga aatgttattg ttagaagacc ttcaaaatca atggtagaag
     6841 gaattacatc atcgcctgag ttaggtaaac ccgattatga acttgcactg aaacagcatg
     6901 atgcatatat cgaggcactg aaggcatgtg gagttaaagt gactgttctg gaggcgcttg
     6961 aagagtatcc ggattcttgt tttgttgagg acacagctgt tcttacacca aaatgtgcta
     7021 ttattactaa tcccggggca ctttccagaa ggggagaatc agcacttatg gttgatacta
     7081 ttaagaaatt ttataatgaa gatcaaattg agtatataac atccccggga acaattgagg
     7141 gtggagatgt tatgatggtt ggaaatcatt tctatgttgg actatctgca cgtactaatg
     7201 aagaaggctt tagacaattt aaagctgctc ttgaaaaata cggacataca agctcattag
     7261 tttctctcga aaaagtattg catctaaaaa cgggagtaaa ttatattgaa aataacatta
     7321 tgttaatttc tggagaattc attgaaaaag aagaatttaa atcatttaat aaaattatag
     7381 ttcctgagaa tgaagcttat ggtgcaaatt gcatttgggt taatgataca gttatagtgc
     7441 cggaaggtta cccaactgtt gaaaaaggtg taagagatac aggatataaa gttattgtag
     7501 tagatacgtc agaatataga aaattagacg gtggattgag ctgtttatca ttaagattct
     7561 aacaaataca tcattatata cgcaaatgct aaaaaattat gagagataat attgcaggaa
     7621 attaagacgg ataaggaaag cgttaataga atgttattca aaggaggttt atatgaaaca
     7681 taaatttata gctaaaagat attggaatac gaaatcatct gcaatgggag aaataggtcc
     7741 tttaatgagc aaatataatg atgttattga tttgacattt ggagacccgg actttactac
     7801 ggatgaaatt attatcaagg aagcatttaa ggatgcactt aatggtcata cacattatac
     7861 aagtttttat ggagacgatg aattaagaga tgaaatatgt aagtattatc atgatgagtt
     7921 caatataaaa gttgaacgaa atgaaacatt tatctcaaca agtgctgccc aaggtttatg
     7981 gcttgcactt gagaccataa cagatgatgg cgatgaaatt attattcatg aaccatactt
     8041 tactccttat cccgatcaaa taaagctaac aagaggcata ccggtttttt tggaaacatt
     8101 tgaagaagag ggttttcaaa tcaatgttga aagattggaa aaattaatta caaatcgtac
     8161 aaaagcaata ataataaatt cacccaacaa tccttcagga gcaaggttta gtgatgaaac
     8221 attattaaaa atagcagaca tagctgaaaa atatgatctt atcgtaatag ttgatgatat
     8281 ttatggatta tatacctttg acagtgaaaa tagaaacaca ccatttatga gtatcaaaaa
     8341 tatgaaagaa agaacaatta ctatatgcag tgcttcgaaa gattatgcaa tgaccggatg
     8401 gcgcatcgga tatttaatag ctccggatta tttagtggat gcaatggaaa aagtaaatga
     8461 aaacaatgta tttacagcgc catccgtttc tcaaagagca atgttacatg ctattagaaa
     8521 tagaaagatt attcaaccaa aactttttaa tatttttaag gaaagagttt tttataccta
     8581 tgacaggata tgtcaaacaa aaaatatgag tgtcttacca ccaagaggta cattttattt
     8641 attccctaat attaaaaaga caggtttatc ttctcaagag gtttctatga agattcttga
     8701 tgaagcacat gttcttacaa tacctggaag tggttttgga agtagcggag aagggtatat
     8761 aaggatagca tgtacagttg gtattgataa attaggcgaa gcatttgaca gaataaataa
     8821 aatggatatt tttaggtaat tgttttgttt gaaaggatga agttatgaat atttgtatta
     8881 tagatgctaa gcagtttata acagatgaaa aacttataga acccttgtat aaattaggaa
     8941 atgtttcaat gtatgatggc ataccccaaa gtttggatga aacaatcaaa agggctaaag
     9001 atgcagatat cgttttattt gcgcttatga agtttacaaa tgaaataatt gataatctac
     9061 caaacttaaa gattttgcaa tttataggta cgggagtttg gaattttgtt gatgcgttgt
     9121 atgctgagaa aaaaggcatt aaggttttaa acattgaagg ttacggtaat aacgccgtag
     9181 cagaatttgc tttaagctgc gcattttctt tagccagaaa aattacaaat gcagacagaa
     9241 tattaaaaga aagaaggtgg tctttggata atctggaagg tattgaaatt gaaggttcag
     9301 tgtttggtgt agtcggtacg ggaaatatcg gctcgatagt agctaaaaag gcgtgtctgt
     9361 taggtgctga tgttattgct tgtgatattt atgaatcgga tgaattgaaa aacaagtatg
     9421 gtattaaata tgtcagttta tctgaaatat ttgaaaaatc tgatattata acaatacata
     9481 tgaaagcaac gaaagaaaca gagaaaatta tcgggaaaga tttattgtct aaaatgaaaa
     9541 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttataaaa agatattatt
     9661 agaggaggat taatatggga cctattgatt taattatttt tgcactttat atgataggga
     9721 tggttgtaat aggatttatt gcacaaggta aaattgaaac aatggatgat tttattcttg
     9781 gagggcgaag atttggttta gttgctttgg ttggtactat tatggcaact atgataggct
     9841 ccggtatgac aatgggtgcg gtaggaaatg cgtacaaagc cggtgccggt ggaacagttt
     9901 tctggatgta tacaggtttt tctattggtt tgctttattt tggttatata gcaggcaaaa
     9961 taagagaaac aggcaaaaga accgttgcag aagtattatc tgctaaattt ggcaataata
    10021 caagattaat atcgtctatc gtaattatag gatatgcggt atcaattgtt gcaataaata
    10081 tagccggttt aagaaatgtt atattctatg tatttggcag caagcttggg atttcattgc
    10141 ccttagccac caccatagcc gcagctatat gtattttata cacatcatta ggtgggtttt
    10201 atgcagttgt ttggacagat gtagttcagt ttggattaat gttgttaggc attgttatat
    10261 taggtcctat tataggtctt aatagcgcag ggggatttaa caatgtagta ttagaatatg
    10321 aaaaaatagg ttcatcaatt acaaatccat tcattaacgg tataagctca ggctcaattg
    10381 gctttttcct cgcatacttt cttactgtac ccggagaccc gacaatgcct caaagagctt
    10441 tagctgcaaa ggatgaaaag tctgcaaaaa tatcattcta tatttcaggt gtgctaggtt
    10501 tctttttcgg atttgcttta ttaattatgg gtggttctgc atttgtgttg atgccaggat
    10561 tagaaaatcc ggaatctgca atgcttttat tcatatcaga atattatcct cctattttaa
    10621 aaggattgtc aatttcagca atagtagctg caattatgtc atcatttgat tccttcttaa
    10681 ttcttgcaac tacacatctt gtttacgatg tgggcagttc attaaataag gatgtaaatg
    10741 aagaaaaaat gaaaaagctt cttccaaagg ttactattgt aataggaatt cttggtttaa
    10801 ttatagctct gtttatacaa tcattatttg actatttgta tatggtattc tcaataattg
    10861 gctcgtccct ggtacctgca ataatagctg cgctttatta cagtgacaaa acgacaagca
    10921 aagcagttgt tgccagtatt gttacaggcg ctgttgttcc cggtgcactc tacttgactt
    10981 acgggtatga tgtattcttg ggagaccctg tatttttagg aattattgca tctaccttag
    11041 tcttgataat tggctctatt gtaactaaag ataagattgc aaccaagtaa ttgacactct
    11101 tttagagctg atgtaaaggt aaaaaccaac aggtcaagaa cttatagttc ttgaccttca
    11161 gttttatgat aaagtttatt ttggttttat tattgctttg aatgacattt ctgtcattat
    11221 gagtacttag ctcgaaaaat cccttgagtt ccttcgttat tgagtagata caaacacata
    11281 aatatttgca gaaatatata tatcatcagg atcattccta tcaacattca tatcgtatat
    11341 aagcaattta actttcaact tatcgttttg cttttcaatt gtaaggcttt ccttatccat
    11401 tgccccttta ggagcatttg acttttcaaa aagcccatct ataaattcct tcatcggtat
    11461 ttcaatcaat gtattggtat catccgaaac ggttatagtt atataaccct tttcgtcagg
    11521 tatctgctta atcgaataat ttttaccttt cagagaaaat tcacctatat cctcgccagg
    11581 tcctcttttg ttttgaatat aaattctgaa aaataaatca taatcttcaa tattaatttt
    11641 ttcattttca tcaagtatgg catatagata aagtgtctct tcctgtcccg gaaatcctct
    11701 gtcataatac tgctcaaacc cgtagatgtt tttgaaattg ccatagtaat tatcggcata
    11761 tttatccggg aaccagtcca tgtcgtccaa atatcccatt ccatacatat aatttgaaat
    11821 attagttatt tcaaacatat catcagtaga aagatctgtt tttttaacaa tttcaccgtc
    11881 tactatcatc ttgtttctgt taagaatttc agtgagtctt ctttcctggc tgtttttaga
    11941 aacggtaaac gcatcaaccg gaggaattat tgacatcagt gcaaagacag cagacaagag
    12001 aacaatagta tttggatttt tctttttgtc gaatatgagc attaatgcac atactattga
    12061 aaatattccg aataacacca cataatatct ggattctgta atgccgtatg catcaatacg
    12121 aatatatgaa gaaaccatct gcattataac taagggtatt agaattaatg gaaacaattt
    12181 tctgtaaagc acagaaaatt tgttattcaa atttgagctc aaaatgtaga tgaaataacc
    12241 ggctgcagaa tatcccaaaa ccataggtcc gacctgtcca acaggccata ctccggttgc
    12301 cagtatttta atgaagtata taatcaagac tgccgtaaat gcggtaataa gcggaattgt
    12361 tatatatgaa accaatatat ccaaaaatct tgggtaggta aatgaaattt ccttttttgc
    12421 atcatcattt tcatcccttg aattaaactt cggcaataag gaaagataat ataaaggagt
    12481 aaagaaaata aatataatat tggctgcatg cgcgtaagat ttgtaatcta cgtcataaag
    12541 caatatatcg atagcaccta ttattgctgc aataccaacg tataaaacta taccgtagag
    12601 aatagccgta aaggctgatt tgaaatgcgc aagtgccaca ttgccaaaat ttaaagcatt
    12661 ttttgatgac ggcaaataca gatatgctgc aaataatgca aaggaaatta ccagcaagtg
    12721 tataagcatt ttgttgtcaa ccttatcatc agtcatgaaa taaaaataac caattgtaag
    12781 taaaatagtt aatccgtaaa ataaaaatga attttttaaa ttctcatatc tttccagcat
    12841 aaatcgaact gccgttgcca aaaacgcacc aaaaatacct gcaaacatat atctcgataa
    12901 taaatctata tccaacgatg aataatcttc aattatcaaa aacataaccg tagacaatga
    12961 agctaagaaa actatagtca taggaaatct tgtaattgtt tctttcaggt tgtaaacaat
    13021 attattcata aagtttttaa tcctgctcat tctatcacct ctttctatat aatttatgac
    13081 gaatttttat aaaatattgt tcctttactt ataaaagtaa tatataccac a
//
LOCUS       DAOPUE010000051        13106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4843_length_13106_cov_10.066049, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000051 DAOPUE010000000
VERSION     DAOPUE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_13106_cov_10.066049"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="PKH42_05975"
     CDS             complement(<1..1496)
                     /locus_tag="PKH42_05975"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOA20013.1"
                     /translation="MQRYEEGKDRSQVQIVNLEDLIDENNPVRVIDAFVDSLNMAKLG
                     FKYAETKETGRKPMNPADMCKLYVYGYYNGIRTSRKLERECEKNIEVMWLINNLKPHN
                     KTISEFRRNNKQVLENVFKEFSMLCDALNLVGKEMVAIDGSKFRASNSRKRNYTKNKV
                     KKMIKYFEESANKYLELLEKNDKDDRDFNEENLTEKLKAAQKRIEELKGMYEDIENNG
                     EISITDKDARHMGVSNNGTDIAHNVQIAVDSENHLVVAVDVVSNPDDSAQLFNMAKQA
                     AKELGIELKDKEENQSEEEYEEYVLTVLADKGYYEYENLRDCLDSGIKPIVAKEKVSN
                     KTGNEKYIRDNFIYDKERDIYICPEGKILNNVSNPTSQNQLYKNKAACKECPFKDQCT
                     TSTEERTIKRNEQHDIYDIVNKIMDENKHVYKRRQEIVEHVFGTVKRNLGYTYFLTRG
                     NESVRAESFMHFLAYNLKRVIKIVGVKALVEAINSYAINIFSFFYKFN"
     gene            1812..3080
                     /locus_tag="PKH42_05980"
     CDS             1812..3080
                     /locus_tag="PKH42_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA20014.1"
                     /translation="MYIHVVQKGDVLWKIADRYNVSIQDIVDLNKLSNPNILLPGHAL
                     LIPTKNTYTVRRGDTIWKIAQKFGVNIQDLLAENRNINPDLIYPGNVLTIPQRPKPEI
                     DVNAFTYFLGSEAVPIVRGAGPYLTYLSPFAYLINEDGTLVNIADEPAIAEAYSQGVV
                     PMMAIVNFTVNVEGENLANIVLNNPDTIDTLQENIINIMREKGYQGLNIDFEYVLPED
                     REAYNSFLESTVNRLHNEGFFVSTSLAPKISGEQVGLLYEAHDYPAHGRLADFVVLMT
                     YEWGWRGGEPRAISPINEIRRVLDYAVTVIPRDKILMGFQIYARDWRIPFQEGDEAET
                     ISVQDAYNLAYEHMSEIHYDYVSQSPYFYYTDAEGNSHVVWFEDARSAQAKFDTVKQY
                     GLRGISYWGLGYEFPQNFPLLADNFQIKKL"
     gene            complement(3114..4439)
                     /locus_tag="PKH42_05985"
     CDS             complement(3114..4439)
                     /locus_tag="PKH42_05985"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOA20015.1"
                     /translation="MDFIIAVIIFIFSLIYNISKQYSLIIPLLIGMLAFSSVAFYRGF
                     KLRNIVVMLMKGMKKSLYILSIFALIGMITALWRAGGTIPFFVYYGIKIMNPDYFILF
                     AFLLTCFVAFALGTCIGTAGTVGVVLIILARSGGVNIYVAAGAILSGAFFGDRSSPLS
                     SSANLVAMITETDLYDNIKKMFITGALPFLATIVVYLLLSKNNPINVTNNELIEKFSV
                     NFNLHISTVLPAVIILLLAISKNDVRVAMLFSIITAVFICLYIQNLTLQEILKYMIFG
                     FVNTDPALSEIISGGGLISMLNVTFVVLIASSYSGIFEGTGMLKDIQGALIKMSNKIG
                     VYLTTVFTSLVTCACCCNQTLSVLLTHDLMNNIYKENNLTKDNLAIDIENTAILIAAI
                     FPWSVAVAVPLATMDVGAKAIFYAVYLFFVPLMAPLIPKSKVYNLGDGN"
     gene            4788..5987
                     /gene="megL"
                     /locus_tag="PKH42_05990"
     CDS             4788..5987
                     /gene="megL"
                     /locus_tag="PKH42_05990"
                     /EC_number="4.4.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018826 - methionine gamma-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009087 - methionine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009093 - cysteine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase"
                     /protein_id="HOA20016.1"
                     /translation="MDKDKLSRMHFSTRAVHAGYRPNEYGALATPIYQTSTFIFDSAE
                     QGGRRFALEEEGYIYTRLGNPTCSQVEDKLASLENAEAAVAMASGMGAITSVIWSIVS
                     AGDHIVAAKTLYGCTFAFMSHGLTKFGVEVDFVDTSDPENVRKAMKPNTKIVYLESPA
                     NPNMLLSDISVISKIAHETEGCMVVVDNTYCTPYIQRPIDLGADVVVHSATKYLNGHG
                     DVIAGFAAGKKEFIDKVRLFGIKDMTGSVMSPFDAYLINRGMKTLEVRMEKHCSNAQK
                     VAEFLESHPAIEWIGYPGLKSFPQYELAKKQMSLPGAMIAFELKGGLESGRKLMNTVE
                     LCSLAVSLGDTETLIQHPASMTHSPYTPEERALSGISEGLVRLSVGLEHADDIIDDLK
                     QALDKLL"
     gene            6195..6383
                     /locus_tag="PKH42_05995"
     CDS             6195..6383
                     /locus_tag="PKH42_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOA20017.1"
                     /translation="MRANVDRDACISCGLCESICPDVFELDDEGISVVKVDPVPSEFE
                     DCAKEAEEECPTDAIHVE"
     gene            6542..7447
                     /locus_tag="PKH42_06000"
     CDS             6542..7447
                     /locus_tag="PKH42_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOA20018.1"
                     /translation="MKYLIGIDSGGTKSELTAYDLNNAQVFNLIGEFGNPAVNLDKTV
                     NNLISLIYDCVNELGREECQLIAVGMAGVETGNYAELIKKYVEGAFVIKTIVLNDAEM
                     ACKAYFGDKDGILAISGTGSSCFAQKDGAGEMVGGWGHILGDEGSGYHTVINVIKNIV
                     YKYDRALPFDDLSKAVMEEIGGSTRSDIMNFIYNNEKNKIASLFPIISDYAEKGDAYA
                     TMLLENAGKYLAELTSVAYKKKNFKGTANIGLKGGVFHNSSIMLSAYKDEIKKRLDSY
                     EIIEEDISTTKAVCNIFKNEKEGAR"
     gene            complement(7505..8308)
                     /locus_tag="PKH42_06005"
     CDS             complement(7505..8308)
                     /locus_tag="PKH42_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOA20019.1"
                     /translation="MGIAHEVDYKIYGDDIQFVEIGLDYGETIVAEPGAMMYMDSSIE
                     MDTMFGDGSEKSKGFKGMLATAGKRVLTGENLFTATFTNTSIDKKVVAFAAPYPGKII
                     PVNLPDYGGRLICQRDAYLCSAKGIEITIAFQKKIGAGLFGGEGFIMQKLVGDGFAFL
                     HAGGTIVKKELASGEMLKLDTGCLVAFTEGVDYDVQFASNVKSALFGGEGLFLATLRG
                     PGTVWMQSLPFSRIADRIYSASRKSGGGGSKGEASLLGNIGIGNLFGGD"
     gene            8470..9294
                     /locus_tag="PKH42_06010"
     CDS             8470..9294
                     /locus_tag="PKH42_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA20020.1"
                     /translation="MDKYEEIERSITKRFRKLIWAKFISAIKEYKLIQDNDKIAVCIS
                     GGKDSMLMAKCMQQLQKYSEIQFETEYLVMDPGYNDANKKRIIENAEILNIPIKIFNT
                     PIFEIVDRVEETPCYLCARMRRGYLYRNARELGCNKIALGHHFDDVIETVLMSLFYAA
                     EIKTMMPKLHSTNHEGMELIRPMYKVREDDIIAWKRYNNLQFIQCACRITERIKAEGT
                     GSKRQEMKILINKLRQINPNIDTNIFKSIHNVNLDTIIGYRKGNVYRNFLDDYDKN"
     gene            9329..10390
                     /locus_tag="PKH42_06015"
     CDS             9329..10390
                     /locus_tag="PKH42_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOA20021.1"
                     /translation="MARGGGSFGGGGRGGGSFGGSRGGGSFGGSRGGGSFGGRSGGSF
                     GGRSGGSFRGSSSGGIFGSSKSTGTGFGPVFGPRIPMGGGFGGGRPQRSSGGGGCGCV
                     TLIVVLIVIAVIFIVIFSLWGSMNSSGGGDITFSSVKRDPLPKGSVNETEYYTDRLGW
                     INNETKLISGLKHFYEKTGVQPYLYLTDTINGSHFPTEQDLEAFATDLYDELFTDEAH
                     LLIVFFEYEGQYKGWYIVGTQAKSVIDAEAGNILADYIDRYYYDSSLSNEEFFSKAFT
                     DAADRIMTVTRSPWITVFIVLGVVVLVIVLFIWWKKHKEQKNLEAKRREEMLNTPLER
                     FGTTEAEDLMKKYQDDNEQ"
     gene            10429..11106
                     /locus_tag="PKH42_06020"
     CDS             10429..11106
                     /locus_tag="PKH42_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOA20022.1"
                     /translation="MAILERFGDIIKANINVLLEKMEDPEKMIDQYLEDMMKDLAEVK
                     QSTAEVIAEETRTKRLVEQNEAEVAKYNEFAKKALVAGNEDDARTFINKKQELENAGA
                     GLMTAYAAAHENAIKMRQMHDKLTADIETLRARRQVIKSKVKVAKTQETLNEATQATK
                     KIAGAKSAFERMEDKANRMLDEANAMAELNMEPIDEAKALEEKYAKMGNASVDDEISK
                     LKEELGL"
     gene            complement(11201..12292)
                     /gene="ychF"
                     /locus_tag="PKH42_06025"
     CDS             complement(11201..12292)
                     /gene="ychF"
                     /locus_tag="PKH42_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOA20023.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITSAGADSANYPFCTIEPNIGVVEV
                     PDKRLNFLTKMSNSGKTVPAVIEFFDIAGLVKGASKGEGLGNKFLSHIREVEAVVHVV
                     RCFEDENVIHVETTVDPKRDIEIIELELVLADLEVMSKRLDKLSKMVKNDKSLQAEFE
                     LVQKIVQGLENEKQARYLSYTDEEQKLLKTFNLITSKPVLYVANVDESYLSDPSGNKY
                     VDIVKEYASRDNSEVIVICGKIEEEISVLDEDEKKEFLEEMGLKESGLDKLIRASYRL
                     LGLISFFTTGPMESKAWTIVKGTKAPQAAGKIHSDMERGFIRAEVTKYDDVVKLGSMH
                     AAKEKGLMRLEGKDYVMEDGDIVLFRFNV"
     gene            12550..>13106
                     /locus_tag="PKH42_06030"
     CDS             12550..>13106
                     /locus_tag="PKH42_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOA20024.1"
                     /translation="MNDKQMQSNYIKIVGSIFSNLEYSHEVFGEKFYEFFIEVPRLSE
                     TKDILPVIISERIINDINMDIGNHIVIEGQFRSYNRYEDDSNRLLLRIFVRDIYVPDE
                     TELEEMKRHPNEVFLNGYLCKETKFRTTPFGREITDMLIAVNRSYNKSDYIPCIAWGR
                     NARYCEKLEVGDHIKLWGRIQSRKY"
BASE COUNT         4328 a         2188 c         2452 g         4138 t
ORIGIN      
        1 aaattaaatt tataaaaaaa tgaaaatata tttatggcat aggagtttat agcctctacc
       61 aaggctttta ctcctactat ttttataact ctttttaaat tgtaagctaa aaagtgcata
      121 aaggattcag ctcttacact ttcattacct ctggtaagaa aatatgtata gcctaagttc
      181 ctttttactg ttccaaatac atgttctaca atttcttgtc ttcgtttata tacgtgttta
      241 ttttcatcca ttatcttgtt tacaatgtcg tatatatcat gctgctcatt tcttttgatc
      301 gttctttctt cagtacttgt tgtacattgg tctttaaatg gacattcttt acacgcagct
      361 ttatttttat ataattggtt ctgggatgtt ggattactaa cattattcaa tatttttcct
      421 tccggacaaa tatatatgtc tctttcctta tcatatatga aattatctct tatgtatttt
      481 tcgttacctg tcttgttaga aactttttct tttgcaacaa ttggttttat tccagaatct
      541 aaacagtccc ttaaattttc atattcataa tatcctttgt cagctaaaac agttaataca
      601 tattcttcat attcttcttc actttgattt tcttctttat cttttaactc tattcctaat
      661 tcctttgctg cctgttttgc catgttgaat aattgtgccg aatcatcagg attgcttact
      721 acatctactg ccacaactaa atgattttcg ctatctactg ctatttgtac attatgggca
      781 atatctgttc cgttattact tacacccatg tgtctggcat ccttatctgt tattgatatt
      841 tcgccattat tttcaatatc ttcgtacatt ccctttaatt cttctattct tttttgagct
      901 gctttaagtt tttctgttaa gttttcttca ttaaaatctc tgtcgtcttt atcgtttttc
      961 tcaagtaatt ccaaatattt atttgcagat tcttcaaaat attttatcat tttctttact
     1021 ttgttcttgg tataatttct ttttctgctg ttagatgcac gaaatttgct tccatctatt
     1081 gcaaccattt ctttcccgac taaatttaac gcatcgcata gcatgctgaa ttctttaaac
     1141 acattttcta aaacctgttt attattcctt ctgaactctg atatggtctt attatgtggt
     1201 ttcagattat ttatcaacca cattacctct atattttttt cacattctct ttctaacttt
     1261 ctggatgttc ttataccgtt gtaatatcca taaacgtata atttgcacat atcagcagga
     1321 ttcattggct ttcttccggt ttctttcgtt tctgcatact tgaatcctaa ttttgccata
     1381 tttaaactgt caacaaatgc atctattact ctaacaggat tattttcatc tattaaatct
     1441 tctaaattaa caatctgtac ttgacttcta tcttttcctt cttcgtatct ttgcactatt
     1501 aacaccccgt ttttgatttg attataccac aaaattattg gaatttcaat ggtttttgtg
     1561 gtatttatgc gtaattttgg ggtgtttttt cattgatttt gattattgtt tttgcgcagt
     1621 ctgccgtccc cttgtcagat atgttgccgg agaaattact ctccggcaac atttattttt
     1681 atcattatag gtggacaatt gaatatatta taacatgaag agatatatat atgacaaggg
     1741 gacgggggta ttgtcattaa aattgcatta cagtttcatg ttataataac aaagaaataa
     1801 agggggaaac tatgtatatt catgttgttc aaaagggaga tgtattgtgg aaaattgcag
     1861 accgttataa tgtcagcata caagatattg tagatcttaa caaattgagt aatccaaata
     1921 ttttactgcc cggccatgca cttcttatac caacaaaaaa tacctataca gttagacgag
     1981 gagatacgat ttggaaaata gcgcaaaagt ttggggttaa tatccaagac ttactggcgg
     2041 aaaacagaaa tattaatccg gatttgatat atccaggtaa tgtacttaca atacctcaaa
     2101 ggcctaagcc tgaaatagat gtaaatgcat ttacatattt tcttggaagc gaagctgttc
     2161 cgatagtaag aggagcagga ccctatttaa cttatttgag cccttttgca tacctgatta
     2221 atgaagacgg aaccttggtg aatattgccg atgagccggc aatagctgaa gcttattcac
     2281 aaggggtggt gcccatgatg gcaatagtaa attttacagt caatgtggaa ggagaaaatc
     2341 ttgcaaacat agtattgaat aatcctgata cgattgatac attacaggaa aatattataa
     2401 atattatgag agaaaaagga tatcagggac taaacatcga ttttgaatac gttcttccgg
     2461 aagacaggga agcctacaac agttttttgg aaagcaccgt taacagattg cataatgaag
     2521 gattttttgt ttccacatca ttagctccta aaataagcgg agagcaagta gggcttttat
     2581 atgaagcgca cgactatccg gctcatggaa gattggcaga ttttgttgtt ctcatgacct
     2641 acgaatgggg ctggagggga ggagaaccaa gagccatttc tccgataaat gaaataagaa
     2701 gggtgttgga ttacgcagta actgtcattc cgagagataa aatattgatg ggatttcaaa
     2761 tatatgcaag agactggaga attccttttc aagaaggcga tgaagctgaa acaatcagcg
     2821 ttcaggatgc atataattta gcatatgagc atatgtctga aattcattat gattatgtaa
     2881 gtcagtcacc gtatttttat tatacggatg ctgaaggaaa ttctcatgta gtttggtttg
     2941 aagatgcaag aagtgcacag gcaaagtttg atacagtgaa acaatacggt ttaagaggaa
     3001 taagctactg ggggttagga tatgaattcc ctcaaaactt ccccttgctt gccgataatt
     3061 tccaaatcaa aaaactatag gggcaccggg gacgggataa ttttaattgt atatcaattt
     3121 ccgtccccaa ggttgtaaac cttggatttg ggtatcaatg gagccattaa gggaacaaag
     3181 aacagatata ctgcatagaa tattgcttta gcgccaacgt ccatggttgc aagcggtaca
     3241 gctaccgcaa cgctccacgg gaaaatcgca gcaataagaa ttgcggtatt ttcaatatca
     3301 atagctaaat tatcttttgt aaggttattt tctttataaa tattattcat taagtcgtgg
     3361 gtaagcaata cggaaagtgt ttgattacag cagcaagcac aagtgacgag gcttgtaaag
     3421 actgttgtta agtaaacacc tattttattg ctcatcttta ttaaagctcc ctgaatatct
     3481 tttagcatgc cggttccttc aaatatgccg gaatatgatg atgctatcaa tacgacaaat
     3541 gtaacgttga gcattgatat taatccgcct cccgaaataa tttcggaaag cgcagggtca
     3601 gtgttgacaa aaccaaaaat catatatttt aaaatttcct gcaaggtcaa attttgaata
     3661 tacaggcata taaaaacagc agttattata ctaaaaagca ttgctacccg aacgtcattt
     3721 ttggatattg ccaacaagag aattatgacg gcaggaagaa ctgttgatat atgcagattg
     3781 aaattaacgg aaaatttctc tatcaattca ttatttgtga catttatggg attatttttt
     3841 gataataaca aatagacaac tatagttgcg agaaatggca gagcgcctgt aataaacatc
     3901 ttttttatat tatcatacaa atctgtttcc gtaatcatcg ctactaaatt tgcactggag
     3961 gataagggcg agcttcggtc tccaaaaaaa gctcccgata aaatggcgcc tgctgcaaca
     4021 tatatgttaa ctccgccgct tctggccaat attatcaata caactcccac tgttccggca
     4081 gttcctatgc atgtaccaag agcaaatgca acgaaacatg taagtaaaaa tgcaaataat
     4141 ataaaataat ccggattcat aatttttatg ccgtaataaa caaaaaacgg aattgttccg
     4201 ccggcacgcc acagagctgt aatcattcct attaaagcaa aaatagacaa tatatacagt
     4261 gactttttca tgcccttcat taacattaca acaatgtttc taagcttaaa tcccctgtaa
     4321 aaggcaacgg aagaaaaagc aagcatacca atcagtaaag gaataattaa ggaatattgt
     4381 ttcgaaatgt tatatataag agaaaagata aaaattatga ctgctattat aaagtccatt
     4441 aaaatctcca ttaaatatat ttaatataat tatataatca ttccttaaaa aatcaagcaa
     4501 aatccaatta ggcaacatta aattatatcc tgcagcccct tataataatt tgctgtccac
     4561 ttattacctc ttggtgtttg atgaatttaa gtaaaatacc ttatgaaacc gcttttcctg
     4621 gggcaaaatt tttttatatg atgcactttg ttatttaaat gagtggacgg taccatttaa
     4681 atgaatggac aactttttga taaatatgga ttgatttata tttaaaagta gtatacaatt
     4741 tatatataga aacgtttact tatcgtcatt ttttataagg aggaaatatg gataaggaca
     4801 agttaagtag aatgcatttt tctacaaggg cggtacacgc cggttacaga ccaaacgagt
     4861 atggagctct agcaacacca atatatcaaa cttctacatt tatatttgat tcagcagaac
     4921 aaggcggaag aagatttgca ttggaagaag aaggttatat ttataccaga cttggaaatc
     4981 caacatgctc tcaggttgaa gataaattag cttcacttga aaatgctgaa gctgcagttg
     5041 caatggcttc aggtatggga gccattacat ccgttatatg gtctatagta agtgctggag
     5101 accatattgt tgcggctaag acattatatg gatgcacgtt tgctttcatg agccatgggt
     5161 tgacaaaatt cggtgttgaa gtagattttg ttgatacttc agatcctgaa aatgttagga
     5221 aggctatgaa gcctaacact aaaattgtat acttggaaag tccggcaaac ccgaatatgc
     5281 ttttgtccga tatttcggta atttctaaaa tcgctcacga gacagaaggc tgtatggttg
     5341 tggtggataa tacttattgc acaccttata tacaaagacc aattgatctt ggtgcagatg
     5401 tggttgttca ttctgcaaca aagtatttaa atggccacgg cgatgttatc gccggttttg
     5461 cagccggtaa aaaagaattt attgacaagg taagactatt tggaataaaa gatatgacag
     5521 gctcggttat gagtcctttt gatgcatact tgattaacag gggaatgaaa acattagaag
     5581 tcagaatgga aaaacactgc tccaatgccc aaaaggtagc tgaattcctt gagtctcatc
     5641 ctgcaataga gtggataggt tatccgggat taaaaagttt tccgcaatat gaacttgcca
     5701 aaaaacaaat gtctttgccg ggagcaatga ttgcatttga acttaaggga ggtcttgaat
     5761 caggaaggaa attaatgaat accgttgagc tttgctcatt agcagtaagc ttaggcgata
     5821 ccgaaacatt gattcagcat cctgcttcca tgacccattc accttatact cccgaagaaa
     5881 gggctttaag cggaatatct gaaggtcttg tgagactatc ggttggtttg gagcatgcag
     5941 acgatataat tgatgattta aaacaagctt tggataaact gctgtaaatc taagcttata
     6001 gaatgtatca ttattgaaaa tttcaataat gaataaatat attaattttt agaacaatat
     6061 tagagtatcg ctaacggttc tttaaatata aggcagctac aaggaaatat aagcattaga
     6121 taatatttaa tgcgacacca aaagaattaa aatgaattaa taataaaata ttatttgctt
     6181 aaaaggagga aattatgaga gctaatgttg accgagatgc ttgcataagc tgcgggctgt
     6241 gtgaatcaat ttgcccggat gttttcgaac tggacgatga aggtatctct gtagtaaagg
     6301 ttgaccccgt accttcagaa ttcgaggatt gtgcaaaaga agctgaagaa gagtgcccta
     6361 cagatgcaat tcatgtggaa taaatgtatg caagactgct cccctagatt agaggagcag
     6421 ttttattatt tcatataatc tttattgatg agttgtttta actataaata attaataata
     6481 tttataattt atatggaaaa ataaagtagt ttttgctata ctaatatcgg gtggtgagag
     6541 tatgaaatat ttaattggaa ttgattcggg cggaacaaag tcagagctta cggcttatga
     6601 ccttaataat gctcaggttt tcaacttaat aggagaattt ggtaatcctg cggtaaactt
     6661 ggacaagact gtcaacaact taatttcatt aatttatgac tgcgttaatg aattaggaag
     6721 agaagaatgt cagcttatag ctgtcggaat ggcaggtgtt gaaacaggaa actatgctga
     6781 gctgattaaa aaatatgtcg aaggtgcttt tgtaattaag actatagtat tgaatgatgc
     6841 cgaaatggct tgcaaagcat attttggaga taaggacgga attctggcaa tttccggaac
     6901 agggtcttct tgctttgctc aaaaagacgg agcaggtgaa atggttggag gatggggaca
     6961 tattttgggc gatgaaggaa gcggttatca tacggttata aatgttatta aaaatatagt
     7021 ttataaatat gacagggctc ttccctttga tgacttaagc aaggctgtta tggaagaaat
     7081 aggcggctct acacgctcgg atataatgaa ttttatatat aataatgaaa aaaataaaat
     7141 agcatcgctt tttcccataa tttcagatta tgctgagaaa ggagatgctt atgcaaccat
     7201 gctcttagag aatgcaggca aatatcttgc tgagcttaca tcagtggcgt ataaaaagaa
     7261 aaattttaag ggaactgcta atataggtct aaaaggagga gttttccaca acagctctat
     7321 catgctttca gcatataagg atgaaataaa aaaaaggctg gactcttatg aaataataga
     7381 agaagatatt tcaacaacca aggcagtttg taatatattt aagaatgaaa aagaaggtgc
     7441 tcgctgagca ccttcagaaa aaatgtgtat ccccatacct ctaacaaata gggttagggc
     7501 atctttaatc accaccgaat aagttaccta ttccaatatt tccaagtaaa cttgcttctc
     7561 cttttgagcc gcctccgccg ctttttctgc ttgccgaata aattctgtca gctattctgc
     7621 tgaatggcaa tgactgcatc catacagttc cgggacctct taatgtagcc aggaataaac
     7681 cttcacctcc aaacaaggca ctttttacat tgcttgcaaa ttgaacatca tagtctactc
     7741 cttcagtaaa tgccaccaaa catcctgtat ctaatttaag catttctcct gaagctaatt
     7801 cctttttaac aattgtgccg cctgcgtgaa ggaatgcaaa accatctcca accaatttct
     7861 gcattataaa tccttcaccg ccgaaaagtc ctgcaccgat ttttttctgg aatgcaatgg
     7921 ttatttcaat cccttttgca gaacataaat aggcatctcg ttgacaaata agccgtccgc
     7981 cgtaatcagg caaattaaca ggtattattt ttccgggata gggtgctgca aaagcaacta
     8041 cctttttatc aatactcgtg ttagtaaatg ttgcagtaaa taaattttct ccggtaagaa
     8101 cccttttgcc tgctgttgcc aacattccct taaagccctt gcttttttca cttccatctc
     8161 caaacatagt atccatttca atgcttgaat ccatgtacat cattgctccg ggctctgcaa
     8221 cgatagtttc tccgtaatcg agaccgattt ctacaaactg aatatcgtct ccgtaaatct
     8281 tataatccac ctcgtgagct attcccataa atcctccttg tttaaaaagt atttaacccc
     8341 taaatccaat attaggatag caaacatttt catgaaatgc aatataaaaa tatcttgttt
     8401 tatgtagatt atgttaatat aatagagtat aactattatt attactatca tattattaag
     8461 caggacaaaa tggataaata tgaggaaatt gaaagaagta tcacaaaaag gtttagaaaa
     8521 ttaatatggg caaaatttat atcagcaata aaagaatata aattaatcca agacaatgac
     8581 aaaattgcag tgtgcatatc aggcggcaag gattcaatgc ttatggctaa atgcatgcag
     8641 caacttcaaa aatacagtga aattcagttt gaaacagaat atttggtaat ggacccggga
     8701 tacaatgatg ctaacaaaaa acgtatcatt gaaaatgcag aaatattaaa tatcccaata
     8761 aaaattttta acacaccaat atttgaaata gttgacagag ttgaagaaac cccgtgttat
     8821 ttatgcgcaa gaatgagaag aggttatttg tacagaaatg caagagaatt aggctgcaat
     8881 aaaatagcct taggtcacca ttttgacgat gtaattgaaa cagttctaat gagcttattt
     8941 tatgctgcag aaataaaaac aatgatgccc aaacttcaca gtacaaatca tgagggtatg
     9001 gagctgataa gacctatgta taaagtccgg gaagatgata taattgcttg gaaaagatac
     9061 aataatttgc agtttattca atgtgcctgc cgcattacgg aaagaattaa ggctgagggt
     9121 acaggttcta aaaggcagga aatgaaaata ttaataaata agttaagaca aatcaatccg
     9181 aatatcgata caaatatatt taagagcatt cacaatgtga atttagatac aattatcggc
     9241 tatcgaaaag ggaatgtgta ccgtaatttt cttgatgatt atgataaaaa ttaatacagt
     9301 atctttgata taattggagg agatttcatt ggctagaggc ggaggttcat tcggcggcgg
     9361 cggccgtgga ggcggctcat ttggcggtag tcgcggggga ggttcatttg gcggtagccg
     9421 tggcggcggg tcttttggcg gacgcagcgg cggctcattt ggtggacgca gcggcggctc
     9481 attcagaggc tcaagcagcg gaggaatatt tggcagcagt aaaagtaccg gtaccggatt
     9541 tggtccggta tttggtccca gaatccccat gggcggagga ttcggcggcg gtagaccgca
     9601 gaggtcaagc ggtggcggag gctgcggatg cgttacatta attgttgttt taattgtgat
     9661 tgcggttatt tttattgtaa ttttttcatt atggggctcc atgaactcaa gcggcggcgg
     9721 cgacattact ttttcatctg ttaaaagaga tccgctccca aaggggtctg taaatgaaac
     9781 ggagtactat acggatcgcc tgggttggat taataatgaa acaaaactta tcagcggctt
     9841 aaaacatttt tatgagaaaa cgggagttca gccatattta tatttgacgg atacgattaa
     9901 cggttctcat tttccgaccg agcaagattt agaggctttt gcgactgatt tatatgatga
     9961 gttgtttact gatgaagcac atttacttat agtatttttt gaatatgaag gtcagtacaa
    10021 aggctggtac atcgtcggaa cacaggcaaa aagtgtaatt gatgctgaag ccggtaatat
    10081 tttggctgat tatatagaca gatattatta tgatagcagt ttgagcaatg aagaattttt
    10141 cagcaaagct ttcactgatg cagctgacag aataatgacg gtaaccaggt cgccatggat
    10201 tacggtgttt attgtattag gtgtagttgt tttagttatt gtacttttca tatggtggaa
    10261 aaagcataaa gagcaaaaaa atttggaagc aaaaagaagg gaagaaatgc taaatacccc
    10321 tcttgaaagg tttggtacaa cagaagctga agacctgatg aaaaaatatc aggatgataa
    10381 tgaacaataa tctgccaaaa ggcagggtta aattaaaagg aggaaaaaat ggctatattg
    10441 gaaaggtttg gagatattat caaggcaaac ataaatgtat tgctggaaaa aatggaagac
    10501 ccggaaaaaa tgattgacca gtacttggaa gatatgatga aagatcttgc agaggtaaaa
    10561 caaagcacag cagaagttat agcagaagaa acacgtacaa agagattagt tgagcaaaat
    10621 gaggcagaag ttgcaaaata taacgaattt gccaagaaag cattagttgc aggcaatgag
    10681 gatgatgcaa gaacatttat taacaaaaaa caagaactgg aaaatgcggg agcaggacta
    10741 atgactgctt atgcggctgc tcatgaaaat gcaattaaaa tgcgacagat gcatgataag
    10801 ttaactgcag acattgaaac attaagagca aggcgacaag ttatcaaatc aaaagttaaa
    10861 gtagctaaaa cacaggaaac actaaatgaa gccactcagg caacaaagaa aatagccggt
    10921 gcaaaaagtg cttttgagcg tatggaagac aaagcaaaca ggatgttaga cgaagcaaat
    10981 gcaatggcag agctgaatat ggagcccata gatgaggcaa aagctctaga agaaaaatat
    11041 gcaaaaatgg gcaatgcttc agtagacgat gaaataagta agttaaaaga agagttagga
    11101 ttgtaataac caaaagctgc cgaaaaggca gctttttagt aatttcaaat ataagacaac
    11161 aaataattat gtatttctct ctagtaaaga aaaatgacaa ttaaacgttg aatctgaata
    11221 atactatatc tccgtcttcc attacataat ctttaccttc aagacgcatc aatccttttt
    11281 cctttgccgc atgcattgag cctaatttta caacatcatc gtattttgtg acttctgcac
    11341 gtatgaaacc tctttccatg tccgagtgta ttttgcctgc agcttgtgga gctttggtac
    11401 cttttacaat tgtccaagcc tttgattcca ttggtcctgt cgtaaaaaag cttatgagtc
    11461 caagtaagcg gtagcttgct ctaataagtt tatcaagccc tgactctttt agtcccattt
    11521 cctctaaaaa ttctttcttt tcatcctcat ctaaaaccga tatttcttct tctatttttc
    11581 cgcaaataac aattacctcc gaattatccc gagaagcata ctcctttact atatccacgt
    11641 atttattgcc tgaagggtct gaaagatagc tttcatcaac attagctacg tacaaaaccg
    11701 gttttgaggt tatcaaatta aaagttttta aaagcttttg ttcctcgtca gtatagctta
    11761 ggtaacgtgc ctgtttttca ttttcaagcc cttgaacaat tttttgaacc aattcaaatt
    11821 ctgcctgcag ggacttatca tttttaacca tctttgaaag cttatcaagt cgttttgaca
    11881 ttacttccaa atcggctaaa accaattcta attctattat ttctatatct cttttagggt
    11941 cgaccgtagt ttcaacatgt attacatttt cgtcttcaaa acatctcact acatgaacaa
    12001 cagcttcgac ctctcttata tgcgaaagaa atttatttcc taatccttct cctttgcttg
    12061 ccccttttac caagcctgca atatcaaaga attctataac tgcaggaact gtcttccctg
    12121 aattgctcat tttagttaaa aaatttaatc ttttatcggg aacttcaact actccgatat
    12181 taggctctat cgtgcaaaag ggataattag ccgaatctgc acctgctgaa gtaattgcat
    12241 taaacaaggt gcttttgcct acattaggaa gtcctactat acctaatttc atgatggtgt
    12301 gaccatcctt tcttcaaatt tacacgttag tacttacaat aaatgaatat gttcttattt
    12361 gttatcctgt tacgcaaact gatttttaat aatattcatt atataatatt aaattttaaa
    12421 gttcaacatt aaataaattt gcattaggaa tactaaaaaa tagacaactt tttggctttt
    12481 aaagcatata ttttattgca atgaatatta ataaaatata taagacaaat tataggggga
    12541 aaaaattata tgaatgataa acagatgcag tccaattata taaaaatagt cgggagcata
    12601 ttcagcaatt tggagtacag tcatgaagta tttggtgaaa aattttacga attttttata
    12661 gaagttccaa gattaagtga aaccaaggat atactgcctg ttattatatc cgaaaggata
    12721 attaatgata taaatatgga cattggaaat cacatagtaa ttgaaggtca gttcaggtca
    12781 tacaaccgat atgaggatga ttcaaacagg cttttgttaa gaatatttgt aagagatata
    12841 tatgtacctg atgaaactga acttgaagaa atgaaaaggc atccaaacga agttttttta
    12901 aacggatatc tttgcaaaga aactaaattc aggactactc cgttcggcag ggaaattact
    12961 gatatgctga ttgcagtcaa tcgttcatat aataagtccg attacatacc ttgcattgca
    13021 tgggggagga atgcaaggta ttgtgaaaag ttggaggttg gagatcatat taaactttgg
    13081 ggaagaatac aaagcaggaa atacca
//
LOCUS       DAOPUE010000052        12836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_4970_length_12836_cov_10.359987, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000052 DAOPUE010000000
VERSION     DAOPUE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4970_length_12836_cov_10.359987"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKH42_06035"
     CDS             complement(<1..789)
                     /locus_tag="PKH42_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA20025.1"
                     /translation="MTKKIVISAVNIRKEYVVKKHLFTGKIKEKYVAVKGAAFDVEEG
                     KTIGIVGESGSGKSTIGEIVGGLQKPTYGCVLYFGKNIKELSEEEYHDYRKSVQFIFQ
                     DPKGSMDPNYEIIDILTMPLLTTNIAKNKDEAEKLIDKMLLRVGLDLTIKNKYPAEVS
                     GGQCQRAAIARALLVNPKLIICDEPVSALDVSIQAQILNLLRELQRELNISYIFISHD
                     IGVVNYMSDTILVMQNGNIVERGEAKEVLLHPKKEYTKTLIESAF"
     gene            complement(798..1748)
                     /locus_tag="PKH42_06040"
     CDS             complement(798..1748)
                     /locus_tag="PKH42_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20026.1"
                     /translation="MNETVVLNNLHIKAGSNTLVKNTSLIINEGEIFGLVGESGSGKT
                     ITAKAIINLLPKGLSYSADEIITAGKNMLTASAKEKKEHIGKNIGYIPQNTVFYLHPM
                     IKIKNQIGDGYIHHMNKTKKEALEKAEALLYKVGFKYPKIVLNYYPWQLSGGMRQRCN
                     IAMALMNDPKLIIADEPTTALDSTVQRQVMELFRKINEEMKLSILLISHDLGLVKHYS
                     HSLAVMYAGQIVESGYTNAVFSNPKHPYTQLLIKIIPSLKIKKNEPLTEIPGLISENG
                     RDIEGCLFFNRCPKAMDICKEMVAEHVDDKHEHYYRCNLE"
     gene            complement(1741..2559)
                     /locus_tag="PKH42_06045"
     CDS             complement(1741..2559)
                     /locus_tag="PKH42_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20027.1"
                     /translation="MRKNISLILGIILVLIIIAVWVLSFVYLPYPPNEMNIPNKFLKP
                     GIDSEHILGTDNFGRDILSRIIYGSRNVLLVAISSVTLGALLGIFLGALAAMERFADT
                     IIMRIMDGLMAFPGILLAMMLVTVMERGIKGSIAAISIFMVPVFSRLVYSMILETEAL
                     LYIKAAKSYGSSRKQIFIRHYIPAMLPRLITQFTSAIGSAVMIETSLSFLGLGVQPPY
                     ASWGLMLSESRQFFLGYPYLAVGPGIAIIITVLGFNLIGDGLNDIIINRGNSDE"
     gene            complement(2556..3515)
                     /locus_tag="PKH42_06050"
     CDS             complement(2556..3515)
                     /locus_tag="PKH42_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20028.1"
                     /translation="MFNYVLDRLKSSIVVLFVVSIITFFVLMIIPGNPAQLILGADAT
                     PEAIQELHVAMGLDRPLYQQYFSWLLDLLKLDMGKSYVYGESVTVLIAKSIPVTFSLS
                     IFAMIMAAFIAFLFGMISAIKKDSIADYFSRSIMQLGAAIPSFWIGMVFIVYFGVKLK
                     LFPVSGFIPFEVNFFRFIKSITLPSLVLAIGEIGTLLRIIRSSMLDSLKQDYMDMARV
                     KGLSSFKIYIKYALRGALTAPLTIIGMQFAKLAGGTVVVETIFALPGLGRLVLTAVEH
                     RDIVLLQGLVMFITSTVIIITLIVDISVMFFNPRVKSFQQGDL"
     gene            complement(3540..5036)
                     /locus_tag="PKH42_06055"
     CDS             complement(3540..5036)
                     /locus_tag="PKH42_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20029.1"
                     /translation="MKKIIAMLLIAMLISACTAQENTPEKADVKTELTMGYTTEPEGL
                     DPHRTAAASTFTVTNNIYDTLVGVTPQWEIVPRLAKEWVISDDGMEITFSLRDDVYFH
                     NGRQMTAKDVEYSFTRLKDAESPRARDYENIVKIEVLNDYEIKFITAELDVELLKSFV
                     YPWAAVVPEEEVANLKTEPVGTGAFTLEEWVPQQHLILKRNENYYGDEIKLETVKMVL
                     IPDATGMMAGFQVGDLDIIPLTGDQVTMVEGNENYKIISEPMNAVQIMSLNTKNEYLS
                     NEKVRQAMAMAINKDEVIEASMFGYGAKIGSHLPPTSPDYYDTNNIIEYNPEKAKELL
                     KEAGYENGFEVDMSLPKNYQLHVDAGQVIADQLGRIGIKVNIQLVEWGTWLSEVYGAK
                     NFDTTVVGHTGRLDSYAFLSRYKSDSNDYISLTTGEVDALLEEALKELDENKRKEIYK
                     EIQVILANKLPAIYLQTPHTMLALQKNVEGMKIFPIDFYDYKDVYFTE"
     gene            5422..5694
                     /locus_tag="PKH42_06060"
     CDS             5422..5694
                     /locus_tag="PKH42_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20030.1"
                     /translation="MSKRPGYAATAAILGIIMHNASVHKIFERIFKYSAHKNAKAAMI
                     ISLILIAIISYFIRLGFILFVLLDFASIILFTAAALIYSKFENRQE"
     gene            5698..7413
                     /locus_tag="PKH42_06065"
     CDS             5698..7413
                     /locus_tag="PKH42_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20031.1"
                     /translation="MLKRFIAYYKPVKIIFITDMICAFAVAVCDLFYPMITRNIINIY
                     VPNQEFQLMIKWLVVLGFIYILKFGLNYYITYYGHIMGVKMQAHMRKDIFEHLQDLPF
                     VFFDENKTGTLSSRIINDLMDISELAHHGPEDLFISLVMLIGSFIMLSTINLPLSLIV
                     FSAVPLLLIVAMKLRLKMEDAFMETRVTIGEVNATIENSISGIRVSKAFSNKENEIEK
                     FEKNNSRFQEARRKAYKVMAEFHVGSSFIIDILNIVVLSSGAIFFFIGIINFGDFAAF
                     LLYTGNFLNPIRKLVNFVEQYQSGSSGFKRFIEIMDKDVEKDDPGAADIYDVKGEISF
                     DDVSFAYDDNKEILKDITFDIEAGKTVAIVGPSGGGKTTLCHLIPRFYEIEEGSISID
                     NIDIRKFTRKSLRKNIGIVQQDVFLFTGTILENIQCGKLDATKEEVYKAAKNANIHDF
                     IMSLPEGYDTYVGERGVKLSGGQKQRISIARVFLKNPPIIILDEATSALDNATEFLIQ
                     KALDELSKGRTTIVVAHRLSTIKNADEIIVLTDQGIQEKGRHQELLDKKGMYAGLYNS
                     QFINQ"
     gene            7423..8151
                     /locus_tag="PKH42_06070"
     CDS             7423..8151
                     /locus_tag="PKH42_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOA20032.1"
                     /translation="MKITIIGHWGGFPKANEATSGYLIEHDNYKLLLECGSGVVSSLQ
                     KITDIKNIDAVLITHYHYDHCCDIGPLQYAIQIKTQLKEINAPLPIYAPTGEFFNLLK
                     WENYTYGISFEENSNLTLGPFEISFIKNIHPVEAYSVRIKCGDKVFSFTSDTSYFENL
                     AEFFSNSDLLICECSFYSDMDGLKAGHLNSSQAGMLAEKANVKNLILTHLPHFGDLNN
                     LLTEAKEFYKGEIELASYMMEIDI"
     gene            8173..8394
                     /locus_tag="PKH42_06075"
     CDS             8173..8394
                     /locus_tag="PKH42_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20033.1"
                     /translation="MIAIKGRRKCANCGKRYNYSYAKGEEALKKAKFKGKEENLSTVT
                     NVKVLSLHTYEVTATCPECGYEEVFIYND"
     gene            8471..8941
                     /locus_tag="PKH42_06080"
     CDS             8471..8941
                     /locus_tag="PKH42_06080"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOA20034.1"
                     /translation="MRFFYGERNILRALEEADFWKKQESEHVDVILEIVPDLEEEYIQ
                     KLEEYQEIFNSTHGRIVQLVETAVNCHNILSPELQRDIINIINLAVRQSQVFIDFIGM
                     LLRDSKAVTNNPTAIIVLNHIRRESEYFVGIVTAFLNSSCANNGRYYNDTGFQR"
     gene            complement(9080..10486)
                     /locus_tag="PKH42_06085"
     CDS             complement(9080..10486)
                     /locus_tag="PKH42_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HOA20035.1"
                     /translation="MDKKFRDTGIEVIGEAFWGTHIAQFYESKDDFFSIGCPYIRAGL
                     LNNELCVWVYSNNTNEEEIKSYLEKYINNADSYIKAGQLLLEPHVDWYLTNNSFNEIR
                     VNEHWLECVTKSTKLGYEGLRAVGDTSWLEKCYYRTFANYENKINKMLPELPFIALCL
                     YDENKTDKLQYAEVIKNHSYTIINNENKKELITNIELVIKDKQLNKALEYNKVKAEFF
                     ANISHELRTPLNVILGTVQMMELAKCTSYEKEEKYLKFLKQNSLRLVRLISNLIDITK
                     IDANYFEIHRKNLDIVNFAELITFSVLDYAKCKNINIIFDTDIEEKVISCDPDYIERI
                     LLNLLSNAIKFTPRGGKILVSVHDMNERISISVKDNGIGIPVDKQKNIFNRFEKVDKS
                     LRRENEGSGIGLSLVKLLVNKLNGNIYLKSQPSKGSEFIVEIPCMTTPETSEFNIENT
                     SVNNIVERAKIEFSDIYC"
     gene            complement(10593..11813)
                     /locus_tag="PKH42_06090"
     CDS             complement(10593..11813)
                     /locus_tag="PKH42_06090"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOA20036.1"
                     /translation="MLIKMPKEVETALNILHVNGFEAYIVGGCVRDSIMGKVPSDWDV
                     ATSAKPEEILLCFSQYRTIETGIKHGTITVIINKMQIEITTYRIDGEYSDNRRPDNVL
                     FTDKIEFDLKRRDFTINALAYNKNGVIDLFGGLDDIKNKIVKCVGNSDERFNEDGLRI
                     LRALRFASVLNFDIDENTSCSIYGNKNLLNNISKERINIELNKLLMGSNFQNILLEYR
                     DVIEVFLPEIRDLTKEKFEQRLKAMKKLNSLVLRLTVLLYEIDSPDKILMNLKYDNKT
                     VKTVKLLAENIDEKIYPDRVNIKRWLNKINHVNLKYLINIKKALFDYEYEELAKSEVI
                     MKEIIETNQCYSLKTLAINGQDLIDSGMPKGKNIGIVLNDVLNKVIEGKLENKKDILF
                     NYIYDRRKEIETPN"
     gene            11986..>12836
                     /locus_tag="PKH42_06095"
     CDS             11986..>12836
                     /locus_tag="PKH42_06095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOA20037.1"
                     /translation="MNKFEKISAMINNTPMLEISLKYQGLRRKVYAKAEYYNFTGSIK
                     DRIAYNILKYSYEAGLIKKGDIIAEASSGNTGIAFSAMGAFLENPVHIFMPDWMSEER
                     KKLLESFGATIHLVSKKDGGFKGSVESADKFGAENNAFLPHQFSNLLNAETHYKTTGA
                     EILRQLDAIGKKPDGFVAGVGTGGTIMGVKNALQEKYPKCKAFPLEPKQSPAMSSGDM
                     VSGFHRIDGIGDGMIPEIVKLEQLDNVIRVDDGDAINMARQLALELGIGVGISSGANL
                     IGSIIAQ"
BASE COUNT         4133 a         2351 c         2096 g         4256 t
ORIGIN      
        1 aaatgcgctt tctatgagtg tctttgtgta ttcttttttc gggtgcagga gaacttcctt
       61 agcctcccct ctttcaacaa tatttccgtt ttgcataact aatattgtgt cggacatata
      121 attaactact ccgatatcgt gagatatgaa tatatatgaa atattaagct ctctttgaag
      181 ctccctcagt aaattcagta tctgtgcctg aatggataca tctaaggcag acacaggctc
      241 atcgcatatt attagttttg gattaacaag caatgctctt gctattgctg ccctctggca
      301 ctgccctccg cttacttcgg caggatattt attctttatg gttaaatcaa gacctactct
      361 taaaagcatt ttatcaataa gtttttcagc ttcatcctta ttctttgcaa tattagttgt
      421 taataaaggc atagtcaaaa tatcgataat ttcatagtta gggtccatag agcctttggg
      481 gtcttgaaaa ataaactgta cgctttttct atagtcatgg tattcttctt ccgataattc
      541 tttaatattc ttgccaaaat aaagaacaca gccatatgta ggtttttgaa gccctcctac
      601 tatttcgcct atggtagatt tgccgcttcc cgattctcct acaatcccga tggtttttcc
      661 ttcttcaaca tcgaaggcgg caccttttac ggcaacatat ttttctttta tcttgcctgt
      721 aaaaagatgc tttttaacaa catattcttt tctgatattt actgcagata tcacaatttt
      781 ttttgtcata attgcctcta ttctaaatta catctgtaat agtgctcgtg cttatcatct
      841 acatgctcag caaccatttc tttacatata tccattgcct tggggcatct gttgaaaaac
      901 aaacatcctt cgatatctct gccattttcc gatataagac ccggtatttc tgttaatggc
      961 tcattcttct taattttcaa tgaaggaatg attttaatca gaagctgtgt gtaagggtgt
     1021 ttgggattag aaaaaactgc atttgtgtat cctgattcta caatctgacc ggcatacatt
     1081 accgctaagc tgtggctgta atgttttacc aatcctaagt catgagaaat caaaagtatg
     1141 gacaatttca tctcttcatt tatttttctg aataattcca taacctgacg ttgaacagtg
     1201 ctgtccaaag ccgttgtcgg ctcatctgca ataataagct tcgggtcgtt catcaaagcc
     1261 attgcaatat tgcatctttg acgcatccct ccgcttaatt gccacggata ataattcagc
     1321 actattttgg gatatttaaa tccaacctta tataacaaag cttctgcttt ttccaaagct
     1381 tccttcttgg ttttattcat atgatgaata tatccgtctc cgatttgatt ttttatttta
     1441 atcatgggat gcagataaaa taccgtattt tgaggaatat atccaatatt tttccctata
     1501 tgttcttttt tttcttttgc cgaagcagtt aacatatttt taccggcagt aattatttca
     1561 tcagccgaat agcttaatcc cttaggaagc aagttgataa tagccttagc agtaatagtt
     1621 tttccggagc ctgattctcc tacaagcccg aaaatttctc cttcgttaat aattagtgaa
     1681 gtattcttta ctaatgtatt acttcctgcc tttatatgga gattgttaag cacaacagtt
     1741 tcattcatca gaattccccc tgtttataat aatatcgttc aacccgtctc caataaggtt
     1801 aaatcctaac actgtaatta ttattgcaat tccggggcca acagccaaat acggataacc
     1861 caaaaaaaac tgtcttgatt cacttaacat caagccccag ctggcatatg gaggctgaac
     1921 tccaagtccc aaaaaagaaa gagatgtttc aatcataact gccgagccta tagctgaagt
     1981 aaactgggtg attaatcttg gaagcattgc aggtatgtag tgtctgatga atatttgttt
     2041 tcttgaactc ccgtagcttt ttgcagcttt tatatacaaa agtgcttcag tttctaatat
     2101 catagaatac acaagccttg aaaatacagg taccataaaa atacttatcg ctgctattga
     2161 gcctttaatt cccctttcca taacagtcac cagcatcatt gcgagaagta ttcccggaaa
     2221 agccataagc ccgtccataa tacgcataat tattgtatct gcaaagcgct ccatggcagc
     2281 aagagctccc aaaaatattc cgagcaaggc acccagtgtt acagatgata ttgcaactaa
     2341 caagacattt cttgaaccgt atattatacg gcttagtatg tccctgccga aattatcggt
     2401 acccagaata tgctctgaat caatgcccgg cttcaaaaac ttgttcggaa tgttcatttc
     2461 atttggagga tagggtaaat aaacaaatga tagtacccaa acagctataa taatcaacac
     2521 taatataatt cctaaaatta agcttatatt ttttctcata ggtccccctg ttgaaaggac
     2581 ttaacccttg gattaaaaaa cattaccgat atatcaacaa ttaaagtgat aattattacc
     2641 gttgatgtga taaacataac aagaccctgc aaaagcacaa tatccctgtg ctctacggca
     2701 gtaagaacca gtcttccaag tcccggaagg gcaaatattg tttcaaccac aaccgttcct
     2761 cccgcaagct tggcaaactg cattcctatt attgtcaatg gggcagtcaa tgcacccctt
     2821 aaggcatatt ttatatatat tttaaagctg ctaagccctt ttactcttgc catgtccata
     2881 taatcctgtt taagagaatc gagcatggag cttcttataa ttcttaaaag tgttcctatt
     2941 tctccgatag ccagaacaag agagggtaaa gttatgcttt ttataaatct aaaaaagttg
     3001 acttcaaatg gaataaagcc tgaaacagga aatagtttta gcttcacacc aaagtaaact
     3061 ataaaaacca ttcctatcca gaaggaagga atggctgctc caagctgcat aatgcttctt
     3121 gaaaaataat ctgcaatact gtcctttttg attgccgaaa tcattccaaa aaggaatgct
     3181 ataaatgctg ccataatcat agcaaaaatt gataatgaaa aagttacggg tatacttttt
     3241 gctattaaga cagtaaccga ttcaccatag acgtaggatt tacccatgtc aagttttagc
     3301 aaatccaaaa gccagctgaa atattgctga tagagcggtc tgtcaagtcc catagcaaca
     3361 tgcagctcct gaatcgcttc aggagttgca tccgctccca atattaactg tgcagggttt
     3421 ccgggtatta tcattaacac aaaaaacgta ataatcgaca ctacaaacaa tacaactatt
     3481 gagcttttaa gtctatctaa aacgtaatta aacataagac acctcagagg gcaatttagc
     3541 tattctgtaa aatacacatc cttataatca taaaagtcta taggaaatat cttcattcct
     3601 tcaacatttt tttgaagtgc caacattgtg tgaggagttt gcaaataaat tgcaggaagc
     3661 ttatttgcta aaattacctg tatctctttg taaatttctt ttcttttatt ttcatcaagt
     3721 tctttcagag cttcttccaa aagcgcatca acttctcctg tagtcaagct tatataatcg
     3781 ttgctgtctg acttatatct cgaaagaaat gcatatgaat caagtcttcc agtatgcccg
     3841 acaaccgtgg tatcaaagtt cttagctcca tatacttcgc ttaaccatgt tccccactca
     3901 acaagctgaa tattcacttt tattcctatc cttcccaatt ggtctgcaat tacctgcccc
     3961 gcatcaacat gaagctgata attcttggga agtgacatat caacctcaaa tccgttttca
     4021 tatcctgctt ccttcaaaag ctctttagcc ttttcgggat tatactctat tatattattt
     4081 gtgtcataat aatcaggtga tgtaggaggc aggtgtgagc cgattttagc accatatcca
     4141 aacatggagg cttcaataac ctcatcttta tttatagcca ttgccattgc ctgtctgact
     4201 ttttcattgg aaagatattc attttttgta ttaagagaca taatttgaac ggcattcata
     4261 ggctcagaaa ttattttata attttcattt ccttcaacca ttgttacctg atctcccgtt
     4321 aatggaataa tgtcaagatc tcctacctga aatcccgcca tcatgccggt agcatcaggt
     4381 attaaaacca tcttaacggt ttctaatttt atttcatcac cgtaataatt ttcatttctt
     4441 tttaaaatca aatgttgctg aggtacccat tcttccagag taaaagctcc cgttccgaca
     4501 ggttcggttt ttaagtttgc aacttcttct tccggaacaa cggctgccca tggatataca
     4561 aagctcttta aaagctcaac atccaattct gcagttataa actttatttc atagtcgttt
     4621 agaacttcaa tttttactat attttcataa tctcttgccc tggggctttc agcatcctta
     4681 agcctggtaa atgaatactc cacatctttg gcagtcatct gtcttccgtt atgaaaataa
     4741 acgtcatccc tcaatgagaa tgtaatttcc attccgtcat cagaaataac ccattccttt
     4801 gctagtctgg gcacaatttc ccattgcggc gttacgccta ccaaagtgtc atatatattg
     4861 ttggttacag taaatgtgga agctgcagca gttctgtgag ggtcaagacc ttcgggctcc
     4921 gtagtatatc ccatggtaag ttcagtctta acatctgctt tttcaggtgt gttttcttga
     4981 gctgtgcatg ctgaaatcag cattgcaatt aaaagcattg ctataatttt tttcattgtt
     5041 cttttccttt cagttaataa aatttcattt agaatatctt ttactgaatg ccaataataa
     5101 tatacccgtt ttcaaatctt tataatcaaa tcatacgtat ctgtaataaa tccttgaaaa
     5161 catattatta tttggcaggg accggtatgt ccctcttcct tttctaggtt taacatattt
     5221 tctttataaa gtcaatagag gatatgcata aattgttcgt tgaaaaaatt agcaataaat
     5281 gatattatta tctaaacaaa tagaggtgat attatgtacg aattacgtca acaacagcga
     5341 aagcaaatgc gtgaacacag gtttttttac cactttatat tgggaattgg aatatttgta
     5401 ttttcgcaag gttgctcatt aatgtctaaa aggccgggat atgcagcaac ggcagcaatc
     5461 ttgggaatta ttatgcacaa tgcaagtgtc cataaaatat ttgaaagaat tttcaaatat
     5521 agtgctcaca aaaatgcaaa agctgcaatg ataatttctt taattttaat tgccattata
     5581 agttatttta taagattagg attcatactt ttcgtattat tggattttgc ttctataatt
     5641 ttatttacgg ctgcagcatt aatatattca aaatttgaaa atcgacagga gtaaaaaatg
     5701 cttaaaagat ttatagctta ttataaaccg gtgaaaataa tttttattac cgacatgatt
     5761 tgtgcatttg cagtagcagt atgtgatttg ttttatccca tgataacaag aaacataata
     5821 aacatctatg taccgaatca agaatttcag cttatgataa aatggcttgt tgtattagga
     5881 ttcatataca ttcttaaatt cggacttaat tattatatta catattacgg tcatattatg
     5941 ggagtaaaaa tgcaggctca tatgagaaaa gacatatttg agcatctgca agacctgccc
     6001 tttgtatttt ttgacgaaaa caaaacggga acattatcat cccgaataat aaatgattta
     6061 atggatattt cagaacttgc tcaccacgga cctgaggatt tattcatatc tttggtgatg
     6121 cttataggct cttttattat gttaagcaca atcaacctgc ctctttcatt gattgtattt
     6181 tcagcagttc ctcttttgtt aattgttgca atgaagctta gactcaaaat ggaagatgcc
     6241 tttatggaaa caagggttac gataggtgaa gttaatgcaa ccattgaaaa cagcatatca
     6301 ggaataagag tttccaaagc attcagcaat aaggaaaatg aaattgaaaa attcgaaaaa
     6361 aacaacagca gatttcagga agcaagaaga aaagcctaca aggtaatggc agaatttcat
     6421 gtcggctctt catttataat tgacatttta aatattgttg ttttaagctc gggagccata
     6481 ttctttttta tagggataat caactttgga gattttgcag cgttcttatt gtatacgggg
     6541 aactttttaa accctatacg aaaattagtc aactttgttg agcagtatca atcgggcagc
     6601 tcaggattca aacgatttat cgaaataatg gacaaggatg tcgaaaaaga tgaccctggc
     6661 gctgcagaca tttatgatgt aaagggtgaa ataagttttg atgacgtctc tttcgcttat
     6721 gatgataata aagaaatatt aaaggatata acatttgata ttgaagcagg caagacggtt
     6781 gccattgttg gcccatcggg aggaggcaag acaacccttt gtcatttaat accgcgtttt
     6841 tatgagatag aagagggaag tatcagcatt gataatattg atataagaaa gtttacacga
     6901 aaatctctca gaaagaacat aggaattgtt cagcaggatg tattcttgtt taccggaact
     6961 attcttgaaa atattcaatg cggtaaactc gatgccacca aggaagaagt atacaaagca
     7021 gccaaaaatg ccaacatcca tgattttata atgtctttgc ctgaagggta tgacacttat
     7081 gtgggtgaaa gaggagtaaa attgtcggga ggacaaaaac aaagaatttc catagctcgg
     7141 gtatttctta aaaatccgcc tataataata ttggatgagg caacctccgc tcttgataat
     7201 gcaaccgagt ttttgataca aaaagctttg gatgaattgt caaagggacg aaccacgata
     7261 gttgtagctc atagattatc aacaattaaa aatgccgatg aaattatagt attaacagac
     7321 caaggcatac aggaaaaagg aagacatcag gagcttttgg ataaaaaggg tatgtatgcc
     7381 ggattataca attcacaatt cataaatcaa taaggagaga taatgaaaat aacaataata
     7441 ggtcattggg gtggattccc gaaggctaat gaagcaactt caggatattt gattgaacat
     7501 gataattata aactcctttt agaatgcggc agcggagttg taagctccct gcagaaaatt
     7561 acggatataa agaacatcga tgctgtattg ataacgcatt atcattatga ccattgctgt
     7621 gatataggac ccttgcagta tgcaatacaa atcaagacgc agcttaagga aattaatgct
     7681 ccactgccca tttatgcacc gacgggagaa ttctttaatc tccttaagtg ggaaaattac
     7741 acttacggca taagttttga agaaaacagc aatttaactc tgggaccatt tgaaataagc
     7801 tttattaaaa atattcaccc tgttgaagca tacagcgtta gaataaaatg cggcgataaa
     7861 gtattttcat ttacttcgga tacttcatat tttgaaaact tggcagagtt tttttcaaac
     7921 tctgatttat tgatttgcga atgcagcttt tattcagata tggacggatt aaaggcagga
     7981 catcttaaca gctctcaagc aggaatgctt gcagagaagg caaatgtaaa gaatcttata
     8041 cttacccacc ttcctcattt tggcgattta aataatttat tgactgaagc aaaggaattt
     8101 tataagggtg aaattgaatt agcatcatat atgatggaga tagatatata atactcttag
     8161 ggaagtgaaa ccatgatagc aataaaaggc agacgaaaat gcgcaaattg cggcaaaaga
     8221 tataactatt catacgcaaa aggcgaagaa gctttaaaga aagccaaatt taaaggcaaa
     8281 gaggaaaatc taagcactgt tacaaatgta aaagtgcttt ccctacatac ctacgaagta
     8341 accgctacct gcccggaatg cggctatgaa gaagtattca tatataatga ttaataaagg
     8401 tatttaacct taataacata aaagaatctc atacgtataa tataccaaat aattatttgg
     8461 aggatgtacg atgagattct tttatggtga aaggaatata ttaagggctt tagaagaagc
     8521 tgatttttgg aagaagcagg aaagtgaaca tgttgatgtt attttagaaa tagttcctga
     8581 cttagaagaa gaatatattc aaaaattaga agaatatcaa gaaatattca attctacaca
     8641 cggaagaata gtccagctcg tggaaacagc tgtaaattgt cacaacatat tatctcccga
     8701 attacagagg gatattatta atataattaa tttagcagta agacaaagcc aggttttcat
     8761 tgactttata ggtatgctgc taagagacag caaagccgtt acaaacaatc ctacggcgat
     8821 aattgttctt aatcacataa gaagagaatc ggaatacttc gtaggaatcg taactgcttt
     8881 cttaaacagc tcttgtgcta ataatggcag atattataat gatacgggat ttcaaagata
     8941 agtgacaaag caattatagg gattaaaaag aaggacttat tacaggggat gggatatata
     9001 agaaggcata aaaaatgggc ggggttagcg tcacaagaat gataataact ccgtccggac
     9061 aagtaaaagt ctttatctct taacaatata tatcagaaaa ctcgatttta gccctttcta
     9121 caatattatt gactgaggtg ttttcaatat taaattctga tgtctcgggt gttgtcatgc
     9181 agggaatttc aacaatgaat tcactgccct tggaaggctg gctttttaaa tatatattcc
     9241 cattgagctt atttactaat aatttaacta aggagaggcc tattccgcta ccttcattct
     9301 ctcttcttaa tgacttgtcc accttctcaa atctgttaaa tatgtttttc tgcttatcaa
     9361 ccggtatgcc gattccatta tcttttactg aaatactgat tctttcattc atatcatgca
     9421 ccgatactaa tattttaccg cccctcggcg taaatttaat agcattagat aagagattca
     9481 gcaaaattcg ttcaatataa tccgggtcgc atgatattac cttttcttca atatctgtat
     9541 caaaaatgat attaatattt ttgcacttag cataatctaa aacagaaaaa gtaattaatt
     9601 ccgcaaaatt tactatatct aagttttttc tgtgaatttc aaaataatta gcatcaattt
     9661 tcgttatatc aattaaattg cttattagtc ttactaatct tagagaattt tgttttaaaa
     9721 attttaaata cttttcttct ttttcataag atgtacattt tgctaactcc atcatttgta
     9781 ctgtccctaa tatgacattt aaaggagttc ttaattcatg agaaatgttg gcaaaaaact
     9841 ccgccttaac tttattgtat tccaatgctt tatttagttg tttatcctta attaccagtt
     9901 caatatttgt tattaattct tttttatttt cgttattgat aattgtataa ctgtgatttt
     9961 tgataacttc cgcatattgc agcttgtccg ttttattctc gtcatatagg cacaaagcaa
    10021 tgaagggtaa ttccggcaac attttattta ttttattctc ataattagca aaggttctgt
    10081 aataacattt ttccagccaa gatgtgtcac cgacagctct aagtccttca taacctagtt
    10141 ttgtagattt tgtgacacat tcaagccaat gttcattaac tcttatttca ttaaaactat
    10201 tattggttaa gtaccaatca acatgtggtt ctaacaacaa ctgccctgcc ttaatataac
    10261 tatccgcatt gttgatatac ttttctaaat aactttttat ttcttcttca tttgtgttgt
    10321 ttgaataaac ccatacacac aattcattgt ttaataaacc tgctcttata taagggcaac
    10381 cgatactaaa aaaatcatcc ttggattcat aaaattgagc tatgtgagtt ccccaaaaag
    10441 cttcaccaat tacttctata cctgtatccc taaatttctt atccatttta tactcccaaa
    10501 tttatcaaca atatatttaa ataaaaattt aggaagatta acaggtattc cagattactt
    10561 caatatttgc atagactttt ctacttaaca tgtcaattcg gtgtttcaat ctcctttctt
    10621 ctatcataaa tgtaattaaa taagatgtct tttttatttt ccaatttgcc ttcaataact
    10681 ttatttaata catcatttaa tactattcct atattcttgc ctttcggcat acctgaatca
    10741 ataaggtcct ggccgttgat tgctaatgtt tttaagctgt aacactggtt agtttcaata
    10801 atttctttca tgattacttc ggacttggcc aactcttcat actcataatc aaacaatgcc
    10861 tttttaatat taataagata ttttagattt acatgattga ttttattgag ccatctctta
    10921 atattcaccc ggtcggggta aattttttca tcaatatttt cagcaagcaa cttaactgtt
    10981 ttaacagtct tgttgtcata ttttaaattc ataagtatct tatcaggcga atcaatttca
    11041 tacaaaagca cagttagtct caaaaccaga gaatttaact tcttcattgc ctttaaacgt
    11101 tgctcaaatt tttcctttgt taaatctcta atttcaggca gaaaaacttc aattacatct
    11161 ctgtattcca aaagaatatt ttgaaaatta cttcccatta acagcttatt taattcaata
    11221 ttaattcttt ccttagaaat attatttagg agatttttgt ttccgtaaat actgcatgat
    11281 gtattctcat caatgtcaaa gttcaaaacg gaagcaaagc gcaatgctcg taaaatccgc
    11341 aaaccgtcct cattgaatct ttcatctgaa ttccccacgc attttacaat tttgtttttt
    11401 atatcatcga gtcctccgaa caagtcgatt actccatttt tgttataagc aagagcatta
    11461 atcgtaaaat ctctccgctt taaatcaaat tcaattttat ccgtaaacaa aacattatcg
    11521 gggcgcctgt tgtcactgta ctccccgtca attctgtaag ttgtaatttc aatctgcatt
    11581 ttatttataa ttactgtaat tgtaccgtgt tttatacctg tttctatggt tctgtactga
    11641 ctaaaacaca gcagtatttc ttccggcttt gctgatgtgg caacatccca atcggaagga
    11701 actttgccca tgatgctgtc cctgacgcat ccccctacta tataagcttc aaagccgttt
    11761 acatgcaata tatttaatgc agtttcaact tcctttggca ttttaataag cataaaatcc
    11821 ttctttttac ctttttatat aattataacc aatcctgtaa ttttaacaag gaaaaaatgt
    11881 cgaaaaaaaa taaaatttcc aaatattttg ttggattttg tgatttgaat ttataaatta
    11941 tattatttta aatgttagtt ataataataa aaaggatatg gtatcatgaa taaatttgaa
    12001 aaaatatcag ctatgattaa taatactccc atgctggaaa tttctttaaa atatcaagga
    12061 ctccggcgaa aagtatacgc taaggcggaa tactacaatt ttaccggcag cattaaagac
    12121 aggattgctt acaatatatt aaaatactcc tatgaagcag gcttaattaa aaagggagat
    12181 ataattgctg aggcatcaag cggcaacacc ggaattgcat tttctgccat gggagcattt
    12241 ttagaaaatc cagtacatat ttttatgccg gattggatga gcgaggaaag aaaaaaacta
    12301 ttggaaagtt tcggtgcaac aatccacttg gtttcaaaga aagatggcgg tttcaaaggc
    12361 tctgtcgaat cagccgataa attcggagct gaaaataatg cctttttacc ccatcagttt
    12421 tcaaatctct taaatgcgga aacccattat aaaacaacag gagctgaaat tttaagacaa
    12481 cttgacgcaa ttggcaaaaa accggatggt tttgttgcag gagtaggtac aggagggacc
    12541 atcatgggag tgaaaaatgc tctgcaagaa aaatacccca aatgtaaggc attccctctt
    12601 gagccaaaac agtcgcccgc catgtcatca ggcgatatgg tttcaggctt tcatagaatt
    12661 gatggaatag gcgacgggat gattcccgaa atcgttaaat tagagcagct ggataatgta
    12721 attcgcgtcg atgacggtga tgcaatcaac atggcaagac agctggcttt agagttagga
    12781 atcggtgtgg gtatttcttc cggtgccaac ttaatcggct ccataatagc acaaaa
//
LOCUS       DAOPUE010000053        12540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5116_length_12540_cov_10.540328, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000053 DAOPUE010000000
VERSION     DAOPUE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5116_length_12540_cov_10.540328"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..509)
                     /locus_tag="PKH42_06100"
     CDS             complement(237..509)
                     /locus_tag="PKH42_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20038.1"
                     /translation="MPINKNVHEQMRNWELQQKQKSQKELREKYEYMNDKYQQMKNSY
                     SGFRNVHKDDNDDAIFDKSSDLADDNSYMRYELNNTFKNKDDKKNK"
     gene            complement(719..1615)
                     /locus_tag="PKH42_06105"
     CDS             complement(719..1615)
                     /locus_tag="PKH42_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA20039.1"
                     /translation="MTKLKADLALLMVTFGWGISYYLTDLSLEDMGPFTLNSNRFLTA
                     FAVAVILAFPKLKNISKRTVKFSFLLGVVLVLVYLGATFGTLYTTLSNVGFLCGLTVI
                     FTPILSSIIYKKLPGKKQTFVILLSFIGIALLTLKDDFSINYDNLLGDLLCIMCAVSY
                     AAHLLITEKAVTFEDVNPFHLGVLQLGTTGVFTLILAFIFETPHLPTQPKYWGPVLFL
                     SIFCTGVAFIVQCVAQQYTEASHVGVIYSLETVFAGIVAFALANDVLSFQSYVGAALM
                     VASIFIMEIDFSKIKIFQKQKQ"
     gene            complement(1846..2877)
                     /locus_tag="PKH42_06110"
     CDS             complement(1846..2877)
                     /locus_tag="PKH42_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA20040.1"
                     /translation="MISIKELEMKKIKDLEAQYPFILSFFENNKLNVEEFKDSTLIEY
                     LNHFTEEEIEEWAIDISKIKSDLETYINQMLQFLGLEKDIVDSITILAGKDKYGNKEN
                     FDELTINKSEIVSIVGPTGSGKSRLLADIEWAANRDTPTERAILINGEKPDYSIRFST
                     GNKLVAQLSQNMNFIMDLTVKEFLELHAKSRMIENEQEVVSKIVEAANELAGEKFNLD
                     TPVTGLSGGQSRALMIADTAILSTSPIVLIDEIENAGIDRKRALNLLVNEEKIVLMAT
                     HDPSLALMADKRIVIKNGGIYKVIETGSKEKEVLKELEKMDKIINTLRFKLRSGDTID
                     TDFGEEMIS"
     gene            complement(2864..3571)
                     /locus_tag="PKH42_06115"
     CDS             complement(2864..3571)
                     /locus_tag="PKH42_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOA20041.1"
                     /translation="MNLVIISGPPSSGKTSVILKTIEAIKKRGIKAGVVKFDCLYTDD
                     DILYEKAGIPVKKGLSGSLCPDHFFASNIEEVVRWGNKEGLDLLITESAGLCNRCSPY
                     IKNIKAVCVIDNLSGINTPKKIGPMLKTADIVVITKGDIVSQAEREVFASRVNSVNPQ
                     AVIMSVNGLTGQGAFELSTLIYDENLNVETVKGSELKFPMPSALCSYCLGETRIGEQY
                     QMGNVRKMNIGEENDKH"
     gene            complement(3568..4758)
                     /locus_tag="PKH42_06120"
     CDS             complement(3568..4758)
                     /locus_tag="PKH42_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20042.1"
                     /translation="MSIDIKDTLFNITEKHKEALDLLISLGFDSLKDDNLRKVFGSSI
                     SLENALKSKKINADEFVTRLMERIEENKSAQKKFKDTIRITGVLPCPVKVPLMETFEK
                     WLSEQNFDFNLNYDLKAASMGIDWIKDEMKNSVNVPDLFISAGFDLFFDKNLLGKYKS
                     ENLFEDITGIQKYNDDFSEYNLEDPSCQYSMLGIVPAVFLVNKDELKGRKIPQSWEEL
                     MSGEFENNVSLPISDFDLFNALLLNIYRLYGEEGIRKLARIFQKNMHPSEMIKSHMKR
                     ERPAITIMPYFFTWMVREGSPMELVWPKDGAIISPIFMLTKKETKDKTKTIADFFASK
                     EAGEILSHKGKMPSVNPEVDNQIPKENKYIWLGWDFIKENDISNLIKKCENIFNDTLK
                     EMTK"
     gene            complement(4937..5794)
                     /locus_tag="PKH42_06125"
     CDS             complement(4937..5794)
                     /locus_tag="PKH42_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOA20043.1"
                     /translation="MYGWMIYPKNTINNKFGNNAFDWMEESALKNGISIDIVFSDDLI
                     ILNNNSKTEFIYGGKSLKFPDFVIMRDYNYTISMQLEILGIRVINSTLSMFNSRNKAV
                     TAQLLVKNNVSTPKFLITKEKNFKFIHNYFDKRKFVMKENEGSQGKGVYLIENEEDYN
                     NVYHKIKDEYFCQEFIESSYGMDIRVYVLGDKVLGCVKRISDSGFKSNYSLGGRVEKY
                     EMTDSIKEISLKAAKAIGLDFCGIDLLFAKDSFTVCEVNGNAGFRTITQVSDIDIPME
                     LFKWVKESL"
     gene            complement(5853..7673)
                     /locus_tag="PKH42_06130"
     CDS             complement(5853..7673)
                     /locus_tag="PKH42_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20044.1"
                     /translation="MKEKQLKPKDPKKTFLRLFSYFKYNLVLFFGGIFFIIVGSSAEI
                     GINGMISPVIDALIGDNDSGLFVKYLLIMVALVVISSIFQYIGNLSMAKLAQKTVYKI
                     REDMFSHMEKLPVSYFDKHSHGELMSTFTNDVDMLNQSLEQSASQILISFVTVVGTFT
                     VMIILSPILTAVVAAMLVVMLYSIKFVSKRSAKNYRMQQAALGDMNGYTEEMMSGQKV
                     VKVFNYEDRAIDTFSKKNEELRNASTLASTYGVMLMPIMGNLSFVMYAIISMLGAYLV
                     MINQLSIGNIASFLLYTRTVSRPITMVSNQLNSLFAALAGAERIFNVLDEKIETDEGD
                     VTLLKDCTGKKALCWMVPKGDGDYEKVPLKGFIEFKDVNFGYSPEKRVLNKIILYAKP
                     GQKIAFVGSTGAGKTTVTNLINRFYEINEGEILYDGIDIKRINKHDLRSTMSIVLQDV
                     HLFEGTVKENIRYGRLDATDDEVVEAAKLANAHYFIKHLPDGYDTVLSSDGQNLSQGE
                     RQLLSIARAAIADPIILILDEATSSVDTRTEKLIAEGMDKLMAGRTTFVIAHRLSTVR
                     NANAIMVIEHGEIIERGDHDELMAQKGRYYALNAGTAELS"
     gene            complement(7670..9391)
                     /locus_tag="PKH42_06135"
     CDS             complement(7670..9391)
                     /locus_tag="PKH42_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20045.1"
                     /translation="MKKLLPFMKKYIKFAVLSPILMILEVIADIAIPYLMSRIVDVGI
                     ANRDIEYVIKIGLIMIAAALLAMISGVISSHYGARAGFGFASEVRLKLYKKIQGFSFA
                     NLDKFTVASLITRLTNDCNTLGQVTMMSLRMAVRAPFMMIFALLMTIRINASLTKVFA
                     VSIPVLVIALIIILSKARPLFLKFQTRVDRVNAIIRENLTAIRVVKSFNRQSHEEKRF
                     KERNDSLMLTALKAITLIVFLMPVLNIIIYSTIIAVLWFGGKQVVAGSMGSGELISFI
                     TYITQILMSFMMMSFFFMQLLRASASVTRIAEIIDTESEIKEVKNPVKELADGSVTFK
                     NVCFCYPGSTEKTLKNINLHINSGEVLGIIGSTGSSKSTLVQLITRLYDATEGSVIVG
                     NVDVKHYSIKTLRDNVAFVLQNNTLFSGTIRDNMKWGNENANDEDIIRALKLAQAWEF
                     VSTYEDGLDHKVEQGGDNFSGGQKQRLTIARALLKSPKIIILDDSTSAVDMATDAKIQ
                     RVFKEELNDVTTIIIAQRVSSIQHADRILVMNEGEIESIGKHEELLVSSPVYREIYES
                     QQKGVGV"
     assembly_gap    9405..9504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9505..11114)
                     /locus_tag="PKH42_06140"
     CDS             complement(<9505..11114)
                     /locus_tag="PKH42_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20046.1"
                     /translation="MRKASEKQRAWNIIWSVSENYSITPDITAYDEKGKADLYWNYII
                     GSVYKYYDYNLLREFFDTLRYDRDYIFYQKVLLLGLEGCIFEKDSKNRPVLAGLRFDY
                     GKKAYEKREEAEVFDEISAAYFSQVFGYDMKVRDNVKSLINELHFDINFNTEQVINKL
                     KQIINKYFEPEYSSEFYIKKIKDNMLLRFFGRKSPSSSLMLIDTDEGRTGTGKIASGW
                     FKFKEKSDNKEREYIKDYFGISAIAESKTKELEKILCDEAHESCHLHFTRGQFDENDD
                     AIFRQKQTSEQRDKNKQHYIKNYIRNTNNITKLTQRIKNAMIVNYTYSMKTESGKLVT
                     EKAWRTVLDDYKIFVRSIKDEIPDMSVDIMLDASASQLNRQEVIAEEGYIIAESLTRC
                     NIPVRIYSFCNMRDFTVMNLFRDYDEKNNNDKIFNYYASGFNRDGMALRTALHMMKDS
                     KYSHRILIVLSDGRPNDMLGVTSKGIIPLQKEYSDETGVSDTANEVRKGRKQGVSILC
                     IFTGRDDDIPSVKKIYGQKFTRILKLDK"
     gene            complement(11196..11507)
                     /locus_tag="PKH42_06145"
     CDS             complement(11196..11507)
                     /locus_tag="PKH42_06145"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HOA20047.1"
                     /translation="MFDENVIKITERAMKHLKKKGKLQITIEIPENRLSSESVLVPIP
                     EIIAKKPKAPENYQIINIGGIDVYISRTLVLPKNDVVIDLDTFLGIKLLRVSGFKSNE
                     G"
     gene            complement(11589..12410)
                     /locus_tag="PKH42_06150"
     CDS             complement(11589..12410)
                     /locus_tag="PKH42_06150"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOA20048.1"
                     /translation="MYIEIIKAIILGIVEGITEWLPISSTGHMILVEEFIKFNASDEF
                     MEMFRVVIQLGAIMAVVLLYFHKLNPFAPSKTPKQKRDTMDIWFKVIVGVLPAAILGF
                     LLDDWFDENFYNYQTVAITLIVYGILFLIIENKNKGKPAKIKSFKELSYKTALITGFF
                     QVLSLVPGTSRSGATILGAIILGTSRNIAAEYSFFLSIPVMFGASALKLVKFGIDFTG
                     LELAVLLTGMTVAFIVSVIAIRFLLRYIKNNDFKAFGWYRIILGILVVGYFMLIS"
BASE COUNT         3635 a         2498 c         1845 g         4462 t
ORIGIN      
        1 tccaatacct tcaacctttg atgagtaaga cgtatattat tcttttttaa ttcatccgat
       61 aattctttta gtgttttcat gcaaacctcg tttataatca tttttatttt gtaatgatta
      121 caatgtaatg atatagtatt atttatttga tgtcaagaga aaaattaaag atttttaaat
      181 aataaaggca aatactcata gtattgaata tttgccttat ttgcctgtaa aactaattat
      241 ttattctttt tatcgtcctt gtttttaaag gtattgttta attcatatct catataactg
      301 ttatcatctg ccaaatcact gcttttgtca aatattgcat catcattatc atctttatga
      361 acatttctga agccgctgta actgttcttc atctgctgat atttatcatt catatattca
      421 tatttttctc ttaattcctt ttgacttttc tgcttctgct gaagctccca attacgcatt
      481 tgctcatgaa catttttatt aattggcata atatcatcct ttcttttata ttattaatga
      541 tattatttgt ttatccaatt aaatttatta caggtataaa agttaaatct gcaagataat
      601 tacagttgta aatctcatca ataagcagag attccggttt aaaattatca aatgtctatt
      661 acattgataa tgaataagct ttgtaagtat acataatgtt atttatgtaa actattattc
      721 attgtttttg tttttgaaat attttaattt ttgaaaaatc aatttccatg atgaagatac
      781 ttgcgaccat aagtgcagca cctacatagg actggaatga aagaacgtca ttggcgagag
      841 caaatgctac aattccggca aacacagttt ccaatgagta tattacacct acatgagatg
      901 cttctgtgta ttgttgagct acgcattgaa caataaatgc tactccggtg cagaaaatcg
      961 ataaaaacaa aacaggtccc caatacttgg gctgtgtcgg caaatgtgga gtttcaaata
     1021 taaaggctag aattaaagtg aatacgccgg tggttccaag ctgcagaaca cctaaatgaa
     1081 agggattcac atcttcaaat gttactgctt tctctgtaat caacaaatgt gcagcataag
     1141 acacagcaca cataatacat aaaaggtctc ccaataaatt gtcatagttt atagagaaat
     1201 catcttttaa tgttaaaagt gctatgccaa taaaactcaa caaaataaca aaggtttgct
     1261 ttttgccggg cagtttctta taaattattg acgataatat gggggtaaaa attacggtaa
     1321 gaccgcataa aaaacctaca ttggacaatg tggtatacaa agtgccgaat gttgctccca
     1381 gataaaccaa taccaatacg acgcccagaa ggaaactgaa tttaactgta cgttttgaaa
     1441 tattcttcaa ttttggaaag gcaagtataa cagcaacagc aaatgctgtt aaaaatctgt
     1501 ttgaatttaa ggtgaagggt cccatatcct ctaaactcaa atcggttaag taatacgaga
     1561 taccccatcc gaatgttacc atcaataaag ccaaatctgc ttttaatttc gtcataatag
     1621 ctgtcccctt ttttacttca tgctatattc taatacatat ttttgaatta ggcaatgaaa
     1681 taaattggga ttccttcagc aaagcactca aaatgacaaa aaaaatgtcg tcctgagtat
     1741 aacggaggat ctcatctttt gagattcttc actgttctca gaatgacact tacctttatt
     1801 catattaata acaaggttgc acctttagaa tgcaggtggg cgtttttatg atatcatttc
     1861 ttcgccaaag tccgtatcta ttgtgtcccc gcttctaagc ttaaatctta atgtattgat
     1921 tattttgtcc atcttttcca attcttttaa aacttctttt tcttttgaac ctgtttcaat
     1981 aaccttataa atgccgccgt ttttaatgac aattctttta tccgccataa gagctaagct
     2041 tgggtcatgg gttgccataa gaactatttt ttcttcatta accaataaat ttaatgctct
     2101 ttttctgtct attcccgcat tttcgatttc atcaatcaac actatgggag aggtgcttaa
     2161 tattgctgta tcggcaatca ttaaagctct tgattgtcct ccgcttaatc ctgttacggg
     2221 tgtatctaaa ttgaattttt caccggctaa ttcgttggct gcttcgacta tttttgaaac
     2281 tacttcctgc tcgttttcaa tcattctgct ctttgcgtga agctccaaaa attccttaac
     2341 ggttaagtcc ataataaaat tcatattttg agacagctga gcaactagtt tgtttccggt
     2401 ggaaaatctt atgctgtaat caggtttttc tccatttatt aatatagctc tttcagtagg
     2461 tgtatccctg tttgctgccc attcaatgtc agccaagagc ctgctttttc cggaacccgt
     2521 aggtcctacg attgaaacaa tttcactttt atttatggta agctcatcaa aattttcttt
     2581 attgccgtac ttgtccttac cggctaaaat ggttattgaa tcaactatgt ctttctctaa
     2641 tcctaaaaat tgaagcattt ggtttatata ggtttctaaa tcggatttta ttttagaaat
     2701 atctattgcc cactcttcga tttcttcttc tgtaaaatgg tttaaatatt caattaaggt
     2761 ggaatcctta aattcttcaa catttaattt gttgttttca aagaaagata atataaaagg
     2821 atactgagct tccaagtctt taattttctt catttccaat tctttaatgc ttatcatttt
     2881 cttctcctat gttcatcttt cttacattgc ccatctggta ttgttcacct attcttgttt
     2941 ctccaagaca gtatgagcac agtgcagaag gcatcggaaa cttaagttcg cttcctttta
     3001 cggtttccac atttaaattt tcatcataaa tcaaggtgct gagctcgaat gctccctggc
     3061 ctgtaagccc attaacggac ataattacag cctgtgggtt tacggaatta acccttgatg
     3121 caaaaacttc cctttctgcc tgagatacaa tatctccctt cgttataacc acaatgtcag
     3181 ctgttttaag cataggacct attttttttg gtgtgtttat accgcttaaa ttatctatta
     3241 cacaaacagc cttaatattc tttatatatg gtgagcatct gttgcataag cctgcacttt
     3301 ccgtaatcaa taaatctaat ccttctttgt ttccccacct cacaacttct tcaatattgc
     3361 tggcgaaaaa gtggtcggga cacaaggagc ccgaaagtcc ctttttaacc ggtatcccgg
     3421 ctttttcata caatatgtca tcatctgtat agaggcagtc aaatttaacc acccctgcct
     3481 taatgcctct ctttttgatt gcctcaattg tttttaaaat tacggatgtt tttcctgaag
     3541 aaggaggtcc tgatattatt actaaattca tttagtcatc tcctttaacg tatcattgaa
     3601 tatattctcg catttcttta ttaagtttga aatgtcattt tcttttataa aatcccatcc
     3661 aagccaaata tatttattct ccttcggtat ctgattgtct acttcaggat ttacgcttgg
     3721 catcttaccc ttgtgagaca gaatttctcc tgcttccttt gaagcaaaga aatcagctat
     3781 tgtctttgtt ttatcttttg tttccttctt tgtaagcatg aaaatagggc ttattattgc
     3841 accgtccttt ggccatacca attccatagg gcttccttct ctcaccatcc aggtgaagaa
     3901 atagggcatt atggtaattg ccggtctttc tcttttcatg tgagacttaa tcatttcaga
     3961 aggatgcata tttttttgga atattcttgc cagtttcctt atgccctctt ctccgtacag
     4021 cctgtaaata ttcaacaata gagcattgaa taaatcaaag tcgcttatgg gaaggctcac
     4081 attgttttca aattcacccg acattaattc ttcccagctt tggggtattt ttctcccctt
     4141 caactcatcc ttgtttacga gaaaaacggc aggaactatt ccaagcattg aatactgaca
     4201 acttgggtct tctagattat attccgaaaa atcatcgtta tatttttgaa ttccggttat
     4261 atcttcaaat aaattttctg atttgtactt tcctaagagg ttcttgtcaa aaaataagtc
     4321 aaaacctgca gatatgaaca aatccggaac atttaccgag tttttcatct catcttttat
     4381 ccagtcaatt cccatagatg ctgcttttaa atcataattt aaattgaaat caaaattttg
     4441 ctcactaagc catttttcaa aagtttccat caaaggaact ttaacagggc agggaagcac
     4501 tccggttatt ctaattgtgt ctttaaattt tttctgagca cttttatttt cttcaattct
     4561 ttccattaat cgagttacaa actcatctgc attaattttc ttagatttta atgcattttc
     4621 caatgagatg gagcttccga acacctttct caggttgtca tctttcaaac tgtcaaatcc
     4681 caaagatatt aataggtcca aggcttcctt atgtttttcg gttatgttga acaaagtatc
     4741 ttttatatct atactcatag tatctccttt tttcaatctt gtaagctaac acctttatgt
     4801 catcctgagc gtaagcgaag gatcacatga gattcttcga gctaaagctc tcagaatgac
     4861 acttcacagg ttattcatac atgatttatt cataagttat tttaacacaa tttaaactta
     4921 attattgtaa catttgttac aaactttcct tcacccattt aaataattcc atgggtatgt
     4981 caatatctga tacctgagta attgttctaa atcctgcatt gccgttcact tcgcatactg
     5041 tgaatgaatc ctttgcaaaa agcaaatcta tgccgcaaaa gtcaagacct atggctttgg
     5101 cggctttaag agagatttct ttgatagaat ctgtcatttc atatttttca actcttccac
     5161 ccaatgaata gtttgactta aagccgctgt cagaaatcct ttttacacat cccaatactt
     5221 tgtctcccag cacatagacg cgaatatcca ttccgtagct ggactcaata aattcctgac
     5281 aaaaatattc atcctttatt ttatggtata cattattata atcttcttcg ttttcaatga
     5341 gataaactcc cttgccctgg gagccttcgt tttctttcat gacaaacttc ctcttgtcaa
     5401 agtaattatg aatgaactta aagttttttt cttttgtgat taagaattta ggagtgctta
     5461 cattgttttt aaccaaaagt tgagcagtaa ctgctttgtt tctggagtta aacattgaca
     5521 gagtggaatt aataaccctt atgcctaaga tttcaagctg catgcttata gtgtaattat
     5581 agtctctcat tattacaaaa tcaggaaatt ttaagctttt accgccgtat atgaattctg
     5641 ttttgctatt gttatttaaa atgattaaat catcgctaaa aacaatatcg atagatatac
     5701 cgttttttaa agcactttct tccatccaat caaatgcatt gtttccaaat ttattattta
     5761 tagtattctt gggataaatc atccatccgt acatatttac ctcctgtata catgaattgg
     5821 tgccatgaat tggtgccagg caccatttgg cattaactca actcagctgt tcctgcattc
     5881 aatgcatagt atcgtccttt ttgtgccatc agttcatcat ggtcgccgcg ctcaataatt
     5941 tcaccatgtt ctattaccat tatggcattg gcattgcgaa ccgttgacaa tctgtgggct
     6001 ataacaaatg ttgttcttcc tgccattaat ttatccattc cttcagctat taatttttca
     6061 gttctagtat ctacggaaga tgttgcttca tcgagaataa gaattatcgg gtcggctatt
     6121 gcagcccttg caattgacaa aagctgcctt tctccctgag ataaattttg tccgtctgaa
     6181 cttagtaccg tatcgtaacc gtcaggaaga tgcttaataa aataatgtgc atttgcaagt
     6241 tttgctgctt ctactacctc atcatccgta gcatccaatc ttccatacct gatattttcc
     6301 ttcactgttc cttcaaacag atgaacatcc tgtaatacta tgctcatggt gcttcgcaaa
     6361 tcatgcttgt taatcctttt tatatctatt ccgtcataga gtatttctcc ctcatttatt
     6421 tcataaaacc tgtttataag atttgtaacg gttgttttgc cggctccggt tgagcctaca
     6481 aaagctatct tttgtccggg ctttgcatag agaattattt tgtttaaaac tcttttttca
     6541 ggcgaatatc caaagttcac atccttaaat tcaataaaac ctttgagagg tactttctca
     6601 taatctccgt caccctttgg aaccatccag caaagagctt tctttcctgt gcaatcttta
     6661 agcaaggtta cgtctccctc atcagtttcg attttttcat ccaatacgtt gaatattctt
     6721 tctgctcctg ccaaggcagc aaacaatgag ttaagttgat ttgaaaccat tgttatgggt
     6781 cttgatactg ttctggtata tagaaggaat gatgctatat tgccgatact cagttggtta
     6841 atcattacta aatatgcccc taacattgat attatagcat acataacaaa ggacagatta
     6901 cccataatag gcatcagcat aacaccgtat gtagaggcca aggtgcttgc attccttaat
     6961 tcttcattct ttttactgaa cgtgtcgata gctctatcct catagttaaa tactttaact
     7021 accttttgac ctgacatcat ttcttcagta taaccgttca tatctcccaa tgcggcttgc
     7081 tgcatgcgat aattttttgc agatcttttg cttacaaatt ttattgaata gagcattaca
     7141 acaagcattg cggcaaccac ggctgtcaaa atcggactta aaataatcat caccgtaaat
     7201 gttccgacaa ctgttacaaa tgatataaga atttgtgaag cactttgttc aagtgattgg
     7261 ttaagcatat ccacatcatt ggtaaaggtg gacattaatt cgccatgaga gtgtttgtca
     7321 aaatatgaaa caggaagttt ttccatatga gaaaacatgt cttcacgtat tttataaact
     7381 gttttttgtg ccaatttcgc cattgacagg ttacctatat attgaaaaat tgagctaatt
     7441 actaccaatg ctaccataat aagaaggtac tttacaaaga gtccgctgtc attgtctccg
     7501 attaaggcat caataacggg actaatcata ccgtttatgc ctatttcagc cgaggagccg
     7561 actattataa agaaaatacc tccaaaaaac aagacaagat tatatttaaa ataagaaaac
     7621 aatcttagaa atgttttttt aggatcttta ggttttaact gtttttcttt catactccca
     7681 cccctttttg ctgagattca taaatttctc tgtatacagg agaagaaacc agcaattctt
     7741 catgtttccc tatagattct atttctcctt cattcattac caaaatcctg tctgcatgtt
     7801 gaatcgaaga aactctttga gcaattatta tagtggttac atcatttaat tcttccttga
     7861 aaacacgctg aatttttgca tctgtcgcca tatctacagc actggttgaa tcgtctaata
     7921 taataatttt aggagatttg agaagagctc gagctattgt aagcctttgt ttctgacctc
     7981 cggaaaaatt atctccaccc tgctctacct tgtggtctaa tccgtcctca taggtggata
     8041 caaattccca agcttgagct aacttaagtg ctctaataat atcttcatca tttgcatttt
     8101 cattgcccca tttcatattg tcgcggatgg ttcctgaaaa taatgtgtta ttctgcagca
     8161 caaaggctac attatcacgc aatgttttta tactgtaatg ctttacatct acattgccca
     8221 caattacgct gccttccgtt gcatcgtaaa gtcttgtaat aagctggacc aaggttgatt
     8281 ttgatgagcc tgtggatccg ataatcccca acacttcacc tgaatttata tgtaaattga
     8341 tattctttaa agttttttcc gtacttccgg gatagcaaaa acatacgttt ttgaaggtca
     8401 cagagccgtc tgccaattcc tttacaggat tttttacttc tttaatttct gactccgtgt
     8461 cgattatctc tgcaatcctt gttacggaag cagaggctct gagaagctgc atgaagaaaa
     8521 atgacatcat cataaaggac atcaaaattt gtgttatata tgtaatgaaa gatattaatt
     8581 ctccgcttcc cattgagcct gcaacaactt gttttcctcc aaaccacaac acggctatta
     8641 tggtagaata aataattata tttaatacag gcattaaaaa cacaataagc gttatcgctt
     8701 ttaatgcagt aagcatcaat gagtcatttc tttctttaaa tcttttttct tcatgactct
     8761 gcctgttgaa tgactttaca acccgaatag cagttaaatt ttcacgtata attgcattta
     8821 ccctgtcaac tcgtgtctga aacttcaaaa aaagcggtct tgcttttgat aaaataataa
     8881 tcaatgcaat aaccaagaca ggtatggaaa ccgcaaatac ctttgtcaaa gatgcattaa
     8941 ttcttattgt catcaaaagt gcaaatatca tcataaacgg agctcttaca gccatcctca
     9001 agctcatcat ggttacctgc cccaatgtgt tgcaatcatt tgtaagcctt gttataagcg
     9061 atgcaaccgt aaatttgtcc aaattcgcaa aagaaaagcc ctgtatcttc ttgtatagct
     9121 ttagcctcac ttccgatgca aaaccaaaac ctgcccttgc accgtaatgt gagcttatta
     9181 caccagaaat catggctaaa agagctgccg caatcattat aagtccgatt ttaataacat
     9241 attcaatgtc acgatttgca atacctacat ccactattct tgacatcaga tatggtattg
     9301 ctatatcggc aataacctcc aatatcatga gtatggggct tagtaccgca aattttatat
     9361 attttttcat aaatggcaat aactttttca atctgttctc ctctnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnaattta tccaacttca atatacgagt gaatttttgc
     9541 ccgtatattt ttttgaccga tggaatatca tcatctcttc ccgtaaaaat acagagaatt
     9601 gaaactccct gtttccggcc ctttctaacc tcatttgcag tatcgcttac gcctgtttca
     9661 tccgaatatt ctttctgcag cggaataata cctttcgaag tcacgcccaa catatcattt
     9721 ggtcttccgt ctgaaagaac aattagtatt ctatggctgt attttgaatc tttcatcata
     9781 tgaagagcag ttcgaagggc cattccgtcc cggttaaagc ccgatgcata gtagttgaaa
     9841 attttatcat tgttgttttt ttcatcataa tcgcggaaaa gattcataac agtgaaatcc
     9901 ctcatgttgc agaaagaata tatccttacg ggaatattgc agcgcgtcag actttctgca
     9961 attatgtagc cctcttctgc tataacttcc tgacgattaa gctgggatgc tgaagcatca
    10021 agcataatat caacggacat atcaggtatt tcatctttaa tacttctgac gaaaattttg
    10081 tagtcatcaa gtacagtcct ccaagctttt tccgtaacaa gctttcctga ttcagtcttc
    10141 attgaatatg tgtaattaac aatcattgca tttttaattc tttgtgtaag ttttgttatg
    10201 ttgtttgtgt tcctgatata attctttata taatgttgtt tatttttatc cctttgctca
    10261 gaagtttgct tctgtctgaa aatcgcatca tcattttcat caaattgccc ccttgtaaaa
    10321 tggagatggc aactttcatg agcttcgtcg caaagtattt tttccaactc tttcgttttg
    10381 gattcggcaa ttgcggaaat tccgaaataa tctttaatat attccctctc cttgttatct
    10441 gacttttctt tgaatttaaa ccatcctgaa gctattttgc cggttccggt tctcccttca
    10501 tcggtatcta taagcatcaa ggagcttgaa ggggattttc ttccgaagaa acgcagcaac
    10561 atattatctt ttattttttt tatataaaat tcactactat attcaggctc aaaatattta
    10621 ttaattattt gcttcaattt atttattact tgctcggtat tgaaatttat gtcaaaatgt
    10681 agctcattta ttaagctctt tacattatct ctgactttca tatcatatcc gaatacttgg
    10741 ctgaagtatg ctgctgaaat ttcatcaaac acttctgcct cttctctttt ttcatacgcc
    10801 tttttgccgt aatcaaaacg aagaccggct aataccggtc tgtttttact atctttttca
    10861 aagatgcatc cttccagtcc aagcagcaat actttctgat aaaaaatata gtctctgtca
    10921 tacctgagag tatcgaaaaa ttctctcaaa aggttatagt cataatattt ataaacagaa
    10981 ccaattatat aattccaata taaatcagct ttaccttttt catcgtacgc agtaatatca
    11041 ggtgttatgg agtaattctc tgaaacactc catattatat tccatgctct ttgtttttcg
    11101 cttgcttttc tcatttgatg attacttcct ttatgcaata tactttccga catttgacat
    11161 acttaattta tgtcatcatc ttattacgaa gcttgttacc cttcgtttga tttaaatccc
    11221 gataccctga gaagttttat acccaagaat gtatccaagt ctataactac atcattttta
    11281 ggcagtacca atgtcctaga tatatatacg tctattccgc caatattgat aatttgatag
    11341 ttttcaggtg ctttcggctt tttggcaata atttccggaa tgggcacaag aacgctttcg
    11401 ctgcttaagc ggttttccgg aatttcaatt gtaatctgaa gctttccttt tttctttagg
    11461 tgcttcattg ccctttcggt tatttttatt acattttcat caaacatttc atactcctta
    11521 taaaagatga gattcttcgg gctgaagccc tcagaatgac acgtagctta ttcatatcaa
    11581 taactttttt agcttattaa catgaaataa cccactacca atatacccaa aataattcta
    11641 taccaaccaa atgctttaaa atcattgttt ttaatatatc ttaacaagaa tcttattgct
    11701 attacagata caataaaggc aactgtcata ccggtcagta atactgctaa ttccaaaccc
    11761 gtgaaatcta ttccgaactt taccaatttt aatgcacttg ctccgaacat tacaggaatt
    11821 gataagaaaa atgaatattc ggctgctata tttctggatg tgccaagtat tatagctcct
    11881 aatatggtag ccccggagcg tgaggttccc ggtaccaggg acaatacctg aaagaagcct
    11941 gttattaatg cggttttata ggataactct ttaaaactct ttatttttgc cggcttacct
    12001 ttatttttat tttcaataat taaaaacaaa ataccgtata caatcagagt tatggctact
    12061 gtctgataat tataaaagtt ttcatcaaac caatcatcga gcaaaaatcc taaaatagcc
    12121 gcaggcagca caccaactat taccttaaac catatatcca ttgtatctct tttttgtttc
    12181 ggtgtcttgc ttggagcaaa tggattcagt ttatggaagt atagcaatac aacggccata
    12241 atggctccca attgtataac aactctgaac atttccataa attcatcgga agcattaaat
    12301 tttataaatt cctcaaccaa aatcatgtga ccggtgctgc taataggaag ccattcggtt
    12361 attccctcta ctattcccaa tattattgct ttgattattt ctatgtacat tcgtcctcca
    12421 accctcttaa ttttataaca catatgaaat tccgcagtct cgttgcacaa ttttgtttgc
    12481 tcctttcgtc gcacaaaatt ggcactcgtt gcgaatgacc taccgctatt tttctctccc
//
LOCUS       DAOPUE010000054        12483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5151_length_12483_cov_9.731413, whole genome shotgun sequence.
ACCESSION   DAOPUE010000054 DAOPUE010000000
VERSION     DAOPUE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12483
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5151_length_12483_cov_9.731413"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PKH42_06155"
     CDS             <1..1436
                     /locus_tag="PKH42_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20049.1"
                     /translation="EEDDRKSVYIDFRLMYDYEKKQIFFNLKTDKQRTITTPQGTTVT
                     NEINYYISKIANNVPYDLLIKIDEAPFNYKIVKSGEFYLLIYISPVDYNDYSTLVEYF
                     GYNFSNNEIYTVYPAKDIYDDNGNYSVLVKSNSNQHLFNINISDIDYRRPSIDTEFSE
                     DNIYLQIEDDFGLDYIITYDGKYVDISGKEAEYKHPEEIIYNGDYIFTITDKKGNRTV
                     ENVEIKSKRKLTTTKSRHAIDLDVHDYKYTEKLFENKGLEYVKSDDEIKLFENIFPAY
                     MSGKNADKFHPDAPITRAEVVTIFCRLNDLPYDNTAYLKSKFTDINNHWARDYISMGS
                     NKRYVSGYKDKTFKPDNYITRAEFCQMLTKISSYKPLLNAIPASSNNPYTDIHNHWAE
                     KEIIKIAGRNLVIGSSDKFYPDEPITRAEVVYAVNTLYGFNPSYSELAYINSLYNKYY
                     NFKDIDNHKYYFYIIISVIGMYREVID"
     gene            1458..1724
                     /locus_tag="PKH42_06160"
     CDS             1458..1724
                     /locus_tag="PKH42_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOA20050.1"
                     /translation="MLLNYMEEIVSRYLEKILKDPAYASICKCDYCKDDIKAMALNNL
                     NPFYITTKRGEVYAEYLSYEIQHQAEIIKEVIKATEFVSLHPNH"
     gene            complement(1914..2396)
                     /locus_tag="PKH42_06165"
     CDS             complement(1914..2396)
                     /locus_tag="PKH42_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOA20051.1"
                     /translation="MRTIVTVGISDMKIVKEHEILISYALGSCVGICIIDCVAQIAGM
                     AHIMLSYNKNGDKKEIFKYADTGITEMVRQMEKLGGIKSRMTAKIAGGAKMFDIKGSS
                     TIGSIGEKNIAAVKDTLRKLRIRLIAEDVGENYGRTIMFDSTNGNLTIKSFGKEEKVI
                     "
     gene            complement(2393..2998)
                     /locus_tag="PKH42_06170"
     CDS             complement(2393..2998)
                     /locus_tag="PKH42_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HOA20052.1"
                     /translation="MKLNESQMDVLKEIGNIGAGNAATSLSNMLSKRIEMNVPEVSLL
                     DYNDIIDSIGGAESIVVGVLIGFEGDIDGVILFLLKKEFVHLILNSLIGTELKNFEEV
                     SEIEMSALSEISNIMVSSYVNSISSLTNMNINVTVPSLNIDMSGALLDSVTAEFSEIA
                     DKAVFIKEKYYCMNETVYSYMLLLPSMPSLKILLNKFGIYT"
     gene            complement(3063..4490)
                     /locus_tag="PKH42_06175"
     CDS             complement(3063..4490)
                     /locus_tag="PKH42_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOA20053.1"
                     /translation="MDKNQQPSYKINHLVSANKLKAYIRIELLDKSEEVEISCDEIFE
                     YLKEQEITYGILENDIIEFCKNKEYAKELIAAGGKEPVNGKDAEIIYNFDISNEKKFA
                     ESDDGTIDFRNLNNVTNVKKDTVLCYIVPAAEGEDGIDVYGNPISYKKGRDISFNYGK
                     NTYISEDGLQLLASTDGCVEFKNNKVYVEDVYKVNNVDNSTGNIDFVGSVIINGDVKE
                     GFSVTAAGDIKIRGMVEGAYIKSDGDVVISKGMNGMSKGSIYAKGNITSKYIENATIV
                     SEKNIYAEALINSDVKAGDSIIIKGLISSIIGGTTAAKNKIYAKTIGNKTNPETNIII
                     DLTDYIEEQKEIEKKIRMNRRLKEELSEKNNKIKEIDEKYELIINSSLDAENKNSVQK
                     QLMLMKIKINNEISEIKKQLQEEIPSDNITEHRIICKGIINSNTRITIGWLKYRVRQD
                     ISYSKIYNDGNEIVILPLNPSDIEP"
     gene            complement(4522..5226)
                     /locus_tag="PKH42_06180"
     CDS             complement(4522..5226)
                     /locus_tag="PKH42_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOA20054.1"
                     /translation="MIKAYYTATNGAISNKNYLDVISNNMANIQTEGFKKSKPEFSDL
                     IYTNMRGTKGADTELKSGSGSKISKVDVVFSQGTPYQTGVKTDFAILGEGFFAVQFEE
                     ENLFTRGGSFEITNIDDENYLTYQGGYILNNQEEPIIIKNSDDIDSIEIGVFTFNNNA
                     DLEQAGNNLYRISNEDAEFNIAEAKVLKGYLEASNVEISDEMVKLIQIQRAFQLNSTV
                     IKTADEIEQTINTLRG"
     gene            complement(5236..5988)
                     /locus_tag="PKH42_06185"
     CDS             complement(5236..5988)
                     /locus_tag="PKH42_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOA20055.1"
                     /translation="MSRGFYTLASGMITQQRKIDVSSNNVANLNTAGYKKEQAITSNF
                     GSLLINKYKQNGIKEEAEPINNISLIRAVTENNTIHSQGVLEETGNITDFAIIGAGFF
                     AIDNEGQILYTRNGSFNIDEEGYLFLEGIGRVQGEFGDIYVEGDKFEFLEDGSIIIEG
                     EIADKIAVFDFPDYNDLNKYGEGTFISDVEPDLAEYPIIKNKTIERSNVEVTEELTNI
                     ISSQRALQTAAQALKIYDSINDKAVNEIGKVQ"
     gene            complement(6012..6794)
                     /locus_tag="PKH42_06190"
     CDS             complement(6012..6794)
                     /locus_tag="PKH42_06190"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOA20056.1"
                     /translation="MILTENKQLQIEEYQNHLWEQYHATGDLTIRNQIIERYIYLVRI
                     IALKMRGIYQQYGDVEDIVNEGIIALMDAIEKFDISKNTKFETYASIRIKGSIIDYVR
                     KQDWIPRKVKNDYKIIKESEELLSNKLGRTPTDNEIVEHLNMDINDYNKIISNAYGTS
                     ILSFEELIEEANLSEYGLKDRHKIPEHEIESKELYKILVESVNKLNEKEKLIISLYYK
                     EELKLKDIAQVLEISNSRVSQIHTSALQKLEKSVRNYLYSDN"
     gene            complement(6811..8853)
                     /gene="flhA"
                     /locus_tag="PKH42_06195"
     CDS             complement(6811..8853)
                     /gene="flhA"
                     /locus_tag="PKH42_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOA20057.1"
                     /translation="MRSIFKNSISIFIIAVILMIIVPLPPFFLDLMFIINIAFAIVIL
                     LTTMYVREALEFSVFPSLLLITTLFRLSLNVSSTRLILTNNGHAGAVIATFGSFVLQG
                     NAVVGFIIFLIIVLVQFIVITKGAERVAEVTARFTLDAMPGKQMAIDADLSSGIITEQ
                     EAMQRRKGVQRESDFYGAMDGATKFVKGDAIMAIVITFINFVGGVIIGMVQGGDTFAG
                     VLQTYSIATVGDGLVSQIPALLISTATSMIVTRAASESSLNVDLTKQIRSQPVVLIIS
                     GVVILFMVFIPGFPLPQVITLSSLLIFLGISLLRARVSAAAETDKDKEEEPVSEEEYF
                     RNIDNIYELIQIDPIAMEVGYSLIPLVDVDGGNFIDRVVMFRRQFAQDMGMVVPSVRL
                     RDNGYLNPNEYVIKVKGEEVAKGEILVEYYLALDPGNLTGEVDGIETIEPAYGIPSKW
                     IKKDKKELAEIYGYTVIDPLSVLITHLSEVVKTHAHELLSRQDLNQLLDKIKKDNKLL
                     VEEVVPGIVSPGNLQKILGNLLKEGIPIKDMETILETISDYGSSVKDTEMLTEYVRQA
                     LKRTITRKWSEGGQIRVITLSTDVEKLIINSISKNDKGSYLSIDPELMQKLVTQLIEQ
                     INKLKGEINIPIILTSPFVRGYFRKLLDQFYPNSVVLSFNEIDNSVQIQALGNVAI"
     gene            complement(8857..9918)
                     /gene="flhB"
                     /locus_tag="PKH42_06200"
     CDS             complement(8857..9918)
                     /gene="flhB"
                     /locus_tag="PKH42_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOA20058.1"
                     /translation="MAGENKTEKASPKKRRDERKKGNVFQSKDVVTVGVIAISFLILN
                     LWAPYIYKYTTNVFYKYVILTKNTYTINDNLIAQAFRDITFTIFASAGLVMFFVITTT
                     VVLTGAQTKFLVSKENIKFKFSKLNPLSGIKRMFSIRSLVEVIKNLIKISILAYVIYS
                     SIANNLFQVPRLISVNLAAGINFIRTSVMSIVKSVVIAFIVISAFDFLYSWWEYEKSI
                     RMTKQEVKEEYKQLEGDPKIRGIIKEKQRAFSMTRMMQQVPKADVIIRNPTHYAVAIS
                     YNIEKDNAPLVLAKGKDRMAARIIEKAEEYKINIIENIPLARALYAESEINREIPVKY
                     YEDVAEILAWVYRLKEKGI"
     gene            complement(9928..10698)
                     /gene="fliR"
                     /locus_tag="PKH42_06205"
     CDS             complement(9928..10698)
                     /gene="fliR"
                     /locus_tag="PKH42_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOA20059.1"
                     /translation="MTFSGDFTYFLLIFARMSGCVFFNQILGRGNLPNIFKISLSLFL
                     AVTTYYILPPEDDMVITTTIEFILLIIKELFIGYLIGYIISLFFSTVVISGEVIGIQI
                     GMSMAKIYDPTRSVSMGIMGSFLNLILILIFFSANGHLNLIKIIITTGKLIEVGNFSI
                     PTHLFYNMVELFQQILILALKLSMPIMAVEIILESGIGILMKAIPQIQVFSVNVQLKI
                     LVGIFLIFILVPTFSTFIENVIVLMFDTVENSLSALIT"
     gene            complement(10711..10977)
                     /gene="fliQ"
                     /locus_tag="PKH42_06210"
     CDS             complement(10711..10977)
                     /gene="fliQ"
                     /locus_tag="PKH42_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOA20060.1"
                     /translation="MSISQVMDVMQAAAGAAIKVGAPILIVSLIIGLSIAVFQAATQI
                     HEQTLTFVPKLLVTAIMLIVLGNWMTEVLSDFTYYVFDLMLRVY"
     gene            complement(11017..11703)
                     /gene="fliP"
                     /locus_tag="PKH42_06215"
     CDS             complement(11017..11703)
                     /gene="fliP"
                     /locus_tag="PKH42_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443404.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOA20061.1"
                     /translation="MNTQAVYGGVINMDINGNSVETLEILFLLTIIALLPSILIMMSS
                     FTRIIIVLSFVKNALGLQQTPPNQVLIGIAIFLTLFTMSPVMEEINTSAYEPYKNEEI
                     SQEEFLDTASIPIKTWMLKQTGSNELQMFLDMSGDEAYKETENIEELPLTIIIPSFIT
                     SELKRAFIIGFLLYIPFLIIDMIVSSVLMSMGMMMLPPVMISMPFKILLFVVVDGWSL
                     LFKTLITTFN"
     gene            complement(11738..12100)
                     /locus_tag="PKH42_06220"
     CDS             complement(11738..12100)
                     /locus_tag="PKH42_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOA20062.1"
                     /translation="MIIINKDIISLIISIIGIIIVLYLTYYCTKWLSTKTTIAAKSRY
                     MNVADKLMLGPNNFITIVEINNKYYLIGITEKDINIIKELEDFHHLPDDKESSNTFNN
                     ILNKYKKTFIGSNNDKKL"
     gene            complement(12097..12465)
                     /locus_tag="PKH42_06225"
     CDS             complement(12097..12465)
                     /locus_tag="PKH42_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOA20063.1"
                     /translation="MEGKILIVDDAAFMRMMIKDTLKSSGYENIIEAADGEQAIQTYK
                     NEKPDLVIMDITMPNKNGLDALKEIRKMDPNAVIVMCSAMGQESMVVEAIRNGAKDFI
                     VKPFKSERIIKTVQSIIDRG"
BASE COUNT         3747 a         2370 c         1787 g         4579 t
ORIGIN      
        1 ctgaggaaga tgacagaaaa agtgtttata tagatttcag gctcatgtat gattatgaaa
       61 aaaaacaaat attctttaat ttgaagactg ataagcaaag gacaataaca acacctcaag
      121 gtacaaccgt cacaaatgaa ataaattatt acataagtaa aatagcaaac aatgtcccct
      181 atgacctgtt aattaaaatt gatgaagcgc cttttaatta taaaattgta aaatcaggag
      241 aattttatct gttaatttat atatcaccgg ttgattataa tgactatagt actcttgtag
      301 aatattttgg ttataatttt tctaataatg aaatttacac tgtatatccg gcaaaagata
      361 tttatgatga taacggaaac tattcggtcc tggtcaaatc aaacagcaat cagcatcttt
      421 tcaatatcaa tatttcagat attgactaca gaagaccgtc aattgacact gaattttctg
      481 aagataatat ttacttacaa attgaagatg acttcggatt agattatatt attacatatg
      541 acggtaaata cgtggatata agcggtaagg aggcagaata taaacatccc gaagaaatca
      601 tttataacgg cgattatatt tttacaatta cagataaaaa aggaaaccga actgttgaaa
      661 atgttgaaat aaagtcgaaa agaaaattaa ctacgaccaa aagcaggcat gcaattgact
      721 tggatgttca cgactataaa tatactgaaa aattatttga aaacaagggc ttagaatatg
      781 taaaatcgga tgatgaaatt aaattattcg aaaatatttt tcctgcctac atgagcggca
      841 agaatgctga taaattccat ccggatgctc cgattaccag ggctgaagtt gttaccatat
      901 tctgtagatt gaatgatttg ccttatgata acactgctta tttaaaatcc aagtttacag
      961 atataaataa tcattgggcc agggattata tatctatggg cagcaacaaa agatatgttt
     1021 caggatataa agataaaact tttaaacctg ataattatat aacaagggct gagttttgcc
     1081 aaatgcttac taaaataagc tcctataagc ctttattaaa tgctattccg gcatcgagca
     1141 acaaccctta taccgatata cataatcact gggcagaaaa agaaataatt aaaattgccg
     1201 gcagaaattt ggttattggc agctcagaca aattttatcc ggatgaaccg attaccagag
     1261 ctgaagttgt ttatgccgta aacacccttt acggatttaa tccaagttat tcggagttgg
     1321 catatatcaa ttccttgtac aataaatact acaattttaa agacatcgac aaccataaat
     1381 actatttcta cattattata tccgtaattg gtatgtatag agaagtaatc gattaattat
     1441 tttgaaggag tacgaaaatg ttgctgaatt atatggaaga aatcgttagc agatatcttg
     1501 aaaagatttt aaaagacccc gcatatgcat cgatatgcaa atgcgattat tgcaaagatg
     1561 atataaaagc tatggcctta aacaatctga atccatttta cattaccact aaaagagggg
     1621 aagtgtacgc agaatactta tcttatgaaa tccaacatca agcagaaata atcaaagaag
     1681 taataaaggc aacagaattt gtttcattgc acccaaacca ctaagatgct ctgtatttca
     1741 cccagataaa gtctgtatca tcctgagtgc atgtgaatga ttttataccc tttgtcatcc
     1801 tgagcgcaag cgaaggatct catgagattc ttcgagctaa agctctcaga atgacagtct
     1861 gcgggttact catagcgatg acagtttgtg tttattcata acaatgacac tttctaaata
     1921 accttttctt cctttccgaa tgatttaatt gttaaattcc cgttagtact gtcaaacatt
     1981 atggtgcggc cgtagttttc gcccacatct tcggcaataa gtcttattct taattttcgt
     2041 aatgtatctt ttacagcagc tatattcttt tcgccgatgc tcccgattgt tgagcttcct
     2101 ttgatatcga acattttagc gccgcctgca atttttgccg tcattcttga ttttatgccg
     2161 cctaactttt ccatttgtct taccatttct gtaatacctg tgtctgcata tttaaaaatt
     2221 tcttttttat cgccattttt attataagat aacataatat gtgccattcc tgctatttgt
     2281 gcaacgcaat cgattatgca aatgccgaca caggagccga gagcatatga tattagtatt
     2341 tcatgctcct taactatttt catgtctgaa attcctacgg ttacaattgt tctcatgtat
     2401 atattccaaa tttgtttaat agaattttta aggatggcat gcttggcaac aaaagcatat
     2461 aactgtatac tgtttcattc atgcaataat atttttcttt aatgaagaca gccttatcgg
     2521 caatttccga aaattctgcg gtaacggaat ccagcagggc acctgacata tcgatattca
     2581 gcgacggaac agtaacatta atattcatat tagtaagaga tgatattgaa ttcacatagg
     2641 aagataccat tatattgctt atttcagaca atgcagacat ttctatttca gatacttctt
     2701 caaaattttt aagctctgtc ccaatcaatg aattcaagat taaatgaaca aattcctttt
     2761 ttaacaaaaa tagtattaca ccatcaatat ctccttcgaa accaatcaat acaccgacaa
     2821 ctatactttc ggctccgcct atggaatcta ttatatcatt gtaatctaat aaacttactt
     2881 caggcacatt catttctatt cttttagaaa gcatattgga aagggatgtc gctgcattgc
     2941 ctgcaccgat atttcctatc tcctttagca catccatttg actttcattt aatttcatgt
     3001 ctttctccct tatgtaagat ttttgtgctg atggaatttt gtcccccctt ataaaaattc
     3061 catcatggct caatatccga aggattaagc ggaagaatta caatttcgtt tccgtcatta
     3121 tatattttac tgtagcttat gtcctgtctt actctatact ttaaccagcc tatggttatt
     3181 cttgtattgg aattaattat acccttacat ataattctat gttctgttat attatcggaa
     3241 ggtatttctt cttgaagctg cttttttatt tcagaaattt cattgtttat ttttattttc
     3301 ataagcatta attgcttctg cacggaattt ttattttcgg catctaagga tgaattaata
     3361 attaattcat atttttcgtc tatttctttt attttattgt tcttttccga taactcttct
     3421 ttcagacggc gattcatcct gatttttttt tctatttcct tttgttcttc gatgtagtct
     3481 gttaaatcaa ttattatatt tgtttcagga tttgttttat tgcctatagt cttggcataa
     3541 attttatttt ttgcagcagt tgttcctccg ataatagaag aaatcagtcc ttttataata
     3601 atagagtcgc cagcttttac gtcgctgttt atcagcgctt ctgcgtaaat atttttttcg
     3661 gatactatgg ttgcattttc aatatacttg cttgttatat ttcctttcgc ataaatgctg
     3721 cctttgctca tgccattcat acccttgctg ataactacgt caccgtcaga ttttatatat
     3781 gcaccttcca ccatacccct tattttaata tctcctgcag cagttaccga aaatccttct
     3841 tttacatctc catttataat tacgcttcct acaaaatcaa tattgcctgt tgaattatcc
     3901 acattattaa ctttataaac atcttctaca tatactttat tatttttaaa ttctacacat
     3961 ccgtcggtgc ttgccaaaag ttgcaggccg tcttctgaaa tatatgtatt tttcccgtaa
     4021 ttaaatgata tatctcttcc ttttttatag ctgatgggat ttccatatac atctattccg
     4081 tcttctccct ctgcagcagg aactatatag cataaaaccg tatccttttt tacgtttgtt
     4141 acattattta agtttctaaa atcaatagtc ccgtcatcgc tttcagcaaa ctttttttca
     4201 tttgaaatat caaaattata aattatctct gcatctttcc cgtttacagg ctccttcccg
     4261 ccggcagcaa taagctcctt tgcatattcc ttatttttgc aaaattcaat tatatcattt
     4321 tctaaaattc catatgtaat ttcttgctct tttaagtatt caaaaatttc gtcacaggaa
     4381 atttcaactt cctctgactt atcaagaagt tcaattctta tatatgcctt cagtttgttt
     4441 gctgatacca gatgattgat tttgtatgag ggttgctggt ttttatccat gactcctcct
     4501 tattttgata tttctccaat cttatcccct taatgtatta atggtttgtt caatttcgtc
     4561 ggcagttttt ataacggttg aattaagctg aaatgctctt tgaatttgaa ttaactttac
     4621 catttcatca gatatttcaa cattggaagc ttccaggtaa cccttcaaaa cctttgcttc
     4681 agctatgtta aactctgcat cttcatttga aattctgtat aggttattac ctgcctgctc
     4741 taaatcagca ttattgttaa aagtaaaaac ccctatctct atactgtcta tatcgtcaga
     4801 atttttaata attataggct cctcctgatt gtttaatata tatcctcctt ggtatgtaag
     4861 gtaattttca tcatcaatat ttgtaatttc aaagctgccg cctcttgtaa acaaattctc
     4921 ctcctcgaat tgaactgcaa aaaaaccttc accaagtatt gcgaagtctg tctttacacc
     4981 ggtttgatat ggagttccct gactgaaaac aacatcaacc ttactgattt tagacccgct
     5041 tcctgatttt aattccgtgt cggctccttt agtccctctc atattggtat agattaaatc
     5101 ggaaaattca ggctttgatt tcttaaaccc ttctgtctgt atgtttgcca tattgtttga
     5161 tataacatcc aaataatttt tattgcttat tgctccgttt gttgccgtat aatatgcttt
     5221 aatcatatag gcctcctatt gtacctttcc tatttcattg actgctttgt catttatcga
     5281 atcatatatt tttagcgctt gagctgctgt ttgcaaagcc ctttgtgatg aaattatatt
     5341 tgttaattct tccgtaactt caacattaga cctttcaata gttttattct ttatgattgg
     5401 atattctgct aagtccggct ctacatccga aataaacgtt ccttcgccat atttgtttaa
     5461 atcattgtaa tcagggaagt cgaaaacagc aattttgtct gcaatttcac cttcgattat
     5521 tatacttccg tcttccagaa attcaaattt atcaccttct acatatatat ctccaaattc
     5581 cccctgtact cttccaatac cttcaagaaa caaatatcct tcttcatcaa tgttaaagct
     5641 tccgttacgg gtgtataaaa tctgcccctc attatcaatt gcaaagaatc ctgctccaat
     5701 aattgcaaaa tccgtaatat ttcctgtttc ctccaaaacc ccctgcgaat gtatggtatt
     5761 gttttccgtt acggctctaa ttagagaaat attgttgatt ggctctgctt cttctttaat
     5821 tccattttgc ttgtatttat ttattagtaa gctgccgaaa tttgaagtta tggcttgttc
     5881 ctttttataa cctgccgtat ttaagtttgc tacattattg cttgatacat cgatttttct
     5941 ttgctgagta atcatacctg aagctaatgt ataaaatcct cttgacatat tgcacctctc
     6001 atctttcatt tttaattatc ggaatataaa tagtttctta cgcttttttc taacttctgt
     6061 aatgccgatg tatgtatttg tgataccctt gaattgctta tttccaatac ttgcgctata
     6121 tccttaagct ttaattcttc cttgtaatat aaagaaatta taagcttttc cttttcatta
     6181 agtttattta cgctttccac cagtatttta tataattcct ttgattcgat ttcatgctca
     6241 ggaatcttat gcctgtcttt taatccgtac tcggataaat tagcttcctc aatcaattcc
     6301 tcaaaggata atatgcttgt accatatgca ttgctgataa ttttgttgta atcgtttatg
     6361 tccatattaa gatgttccac tatttcattg tccgtgggag ttctgcctaa cttattggac
     6421 aagagctctt ctgattcttt tattattttg taatcatttt taacttttcg cggtatccag
     6481 tcttgtttcc ttacatagtc gataatcgac cctttaatcc ttattgaagc atatgtttca
     6541 aatttcgtat ttttggatat atcaaatttc tcgattgcat ccatcagagc tatgattcct
     6601 tcatttacaa tatcctcaac atccccgtac tgttgatata tgcccctcat tttcagggct
     6661 attattctta ctaagtagat gtatctttca ataatttgat ttctgatggt taaatctccg
     6721 gttgcatggt actgctccca taagtggttt tgatattctt caatttgtaa ttgtttgttt
     6781 tcggttaaaa tcatatcatc actctttcat ttatattgct acattgccca aggcctgtat
     6841 ttgtactgaa ttgtcaattt cattaaatga taatacaacg gagttaggat aaaattggtc
     6901 taatagcttt ctgaaatatc ctcttacaaa tggtgaagta agaattatcg ggatgtttat
     6961 ttcacctttc agcttattga tttgctcaat cagctgcgtc actaattttt gcatcaattc
     7021 aggatcaatt gataaatagg agcctttatc atttttactt attgaattga ttattaattt
     7081 ttcaacatca gtgcttaatg taatgaccct gatttgaccg ccctcagacc atttgcgtgt
     7141 tatcgttctt tttagtgctt gtcttacgta ttccgtaagc atttccgtat ccttgacaga
     7201 agacccgtaa tcactgattg tttctaaaat tgtttccata tctttaatag gaatgccttc
     7261 tttaagcaaa ttgcccaata ttttttgcag attgcccgga gatacgattc cgggaacaac
     7321 ttcttccacc aataatttat tatccttctt tattttgtct agcagctggt ttaaatcctg
     7381 tcttgataat aattcatgag catgtgtttt tactacctcc gacaaatgtg ttattaatac
     7441 agacaaagga tctattacgg tatatccgta aatttccgcc aactcttttt tatccttctt
     7501 aatccatttg cttggtattc catatgcagg ctctattgtt tcaataccat ccacttctcc
     7561 cgttaagttt ccggggtcta aggccaagta gtattctact aatatttccc ccttggcaac
     7621 ttcttctcct ttaactttta taacatactc gtttggatta agatatccgt tatctctcaa
     7681 tctgaccgaa ggaactacca ttcccatatc ttgggcaaat tgacgcctga acattactac
     7741 tctgtcaatg aagtttcctc cgtcaacatc taccagcgga attaaactgt atcctacttc
     7801 cattgcaata gggtctattt gaattaattc ataaatatta tcaatatttc tgaaatactc
     7861 ctcttccgat acaggctctt cctctttatc cttatctgtt tcagccgcag cggaaactct
     7921 tgctcttaac aggctgattc ctaaaaaaat cagcagtgat gacagtgtaa ttacctgagg
     7981 caaaggaaat cccggtataa agaccataaa taatattact actccggata ttatcaagac
     8041 taccggctgt gaccttattt gtttggtcaa atctacgttt aagcttgact cggaagcagc
     8101 tcttgtaaca atcatgcttg ttgccgtaga tattagtaga gcaggtatct gagaaaccag
     8161 tccgtcacct acggttgcta tggaataagt ctgaagtact ccggcgaaag tgtcgcctcc
     8221 ctgaaccata cctatgatga caccgcctac aaaatttata aaggtaatta ctattgccat
     8281 tatagcgtcg ccctttacaa attttgttgc accatccatt gctccataaa aatccgattc
     8341 cctttggact cctttacgcc tctgcattgc ttcctgctct gtgattatgc ctgagcttaa
     8401 atcagcatca atggccattt gttttccggg cattgcatcc aaggtaaatc ttgcggtaac
     8461 ttctgctact ctttcggcgc cctttgtaat tactataaat tgaactaaga ctataattaa
     8521 aaatataata aacccgacaa ctgcatttcc ttgtaataca aagcttccga atgtggctat
     8581 aactgcccct gcatgtccat tgtttgttaa gatcaacctt gtggaagata catttaagga
     8641 cagtctgaat agggtagtta ttaaaagaag cgaaggaaat acggaaaatt ccaatgcttc
     8701 ccttacgtac atcgttgtga gcaatataac tatggcaaat gcaatattga taataaacat
     8761 taaatccaaa aagaagggag gtaatggtac tattatcata agtattactg ctataataaa
     8821 tattgaaata gaattcttga atatacttct catatcttat attccttttt ccttaagcct
     8881 ataaacccaa gctaaaattt ctgcaacatc ttcataatat tttacgggta tttcccgatt
     8941 tatttctgac tcggcataaa gagcccttgc caatggaatg ttttcaataa tgttaatttt
     9001 atattcctct gccttctcaa taatccttgc tgccattctg tcttttcctt ttgccagtac
     9061 taggggagca ttgtcttttt caatgttata acttatagca acagcataat gtgtagggtt
     9121 tcttataata acatcggctt ttggtacttg ctgcatcatt cttgtcattg aaaaggctct
     9181 ttgtttttct tttattattc ctcttatttt ggggtcaccc tctaattgct tatactcttc
     9241 cttaacttct tgcttggtca ttctgatact tttttcatat tcccaccagg aatataaaaa
     9301 gtcgaatgca gaaataacta taaaagcaat tacaacactt tttactatgg acataaccga
     9361 tgttcttatg aaattaattc cggcagctag attgactgaa atcaatctgg gtacctgaaa
     9421 taaattgttt gcaatcgaag aatatattac atatgctaat attgatattt taattaaatt
     9481 ctttattacc tccacaagag aacggataga aaacatcctt tttatgccgg aaagcggatt
     9541 taattttgaa aacttaaatt ttatattttc cttagaaact aaaaacttag tttgtgcacc
     9601 ggttaataca acagtcgttg ttattacaaa aaacattacg agtcccgctg atgcaaatat
     9661 ggtaaaggta atatccctga acgcttgagc aataagatta tcatttattg tataagtatt
     9721 ttttgtcagg atcacgtatt tataaaatac atttgtagta tacttataaa tatatggggc
     9781 ccatagattc aatatcaaaa atgatattgc aattactcca acggttacta catccttgct
     9841 ctgaaataca ttaccttttt ttctttcatc ccgtcttttt ttgggggaag ctttttcggt
     9901 tttattttcg cctgccatca cttcacctta tgtaattaat gcgcttagac tgttttctac
     9961 agtatcaaac atcaatacga ttacattttc tataaatgtt gaaaatgtag gtacaagtat
    10021 aaatattaag aatattccca ctaatatttt caactgaaca tttacggaaa aaacctgtat
    10081 ttgcggtatt gccttcatca aaattccaat tcccgactcc aatattattt ctaccgccat
    10141 tatgggcatt gacagtttta atgccagtat taatatttgt tgaaacagtt cgaccatatt
    10201 gtaaaacaga tgagtcggta tggaaaaatt gcctacttct attaatttgc cggtcgttat
    10261 aataattttt atcaaattta aatgaccgtt tgcactgaaa aagataagta ttaaaattag
    10321 atttaagaag cttcccataa tgcccataga aacacttctt gtggggtcat atattttagc
    10381 catggacatt cctatttgta ttcctataac ttcaccgctt ataacaaccg tactgaaaaa
    10441 taagcttatt atataaccta tcaaataacc gataaacaac tcttttataa ttaataatat
    10501 aaattcaatt gtagttgtaa tgaccatgtc atcttcagga ggcagtatat aataggttgt
    10561 tacagccaga aataagctta aggatatttt aaatatattc ggtaaattcc ctctgcccaa
    10621 tatttgatta aaaaaaacac aaccgctcat tcttgcaaat attaataaaa aataagtaaa
    10681 atctcctgaa aatgtcatcg caagtcctca ttaatatact cttaacatta aatcaaatac
    10741 atagtatgta aaatcagaca aaacttcagt catccaattt cccaatacga ttaacattat
    10801 tgcagtaacc aataattttg gaacaaaggt caatgtctgc tcatgtattt gagttgcagc
    10861 ttgaaaaaca gctattgata aacctattat taaacttact atcagtatgg gtgcccctac
    10921 ttttattgca gctcccgcag cagcctgcat aacatccata acctgcgaaa tgctcattat
    10981 tatctccttc tcttaatcag gtattttaaa tacctattaa ttaaacgtcg taattaatgt
    11041 cttaaataat aaagaccacc cgtccacaac aacaaataac agtattttaa acggcattga
    11101 tatcattacc ggcggcagca tcatcatgcc catggacata agaacgcttg aaactatcat
    11161 atctattatt aagaatggga tataaagtaa aaatcctatt ataaatgctc tttttaattc
    11221 actggtaata aaggacggaa taattattgt cagaggaagt tcttcgatat tttcggtttc
    11281 tttataagct tcatctcccg acatatccaa aaacatttgt aattcattgc tgcctgtttg
    11341 tttcagcatc catgttttta tcgggattga agccgtatct aaaaattcct cctgtgaaat
    11401 ttcttcattt ttatatggct cataagcaga tgtgtttatt tcttccatta cgggtgacat
    11461 tgtaaataat gtcaagaata ttgctatgcc tatcagtact tgattaggcg gcgtctgctg
    11521 caatccgagt gcatttttta caaatgataa tacgataatt attcttgtaa aagatgacat
    11581 cattattaag attgacggga gtaaagcaat aattgtcaaa agaaacagaa tttccaatgt
    11641 ctctactgaa ttcccgttta tatccatatt aataactccg ccgtatacag cctgagtatt
    11701 cataagtatt aatattaaga ctataaaaat tgatttatta taacttcttg tcattgttgc
    11761 ttcctataaa tgttttttta tatttgttca aaatattatt gaatgtatta ctgctttctt
    11821 tatcatcggg taaatggtga aaatcttcta attcttttat gatattgata tctttttccg
    11881 taatgcctat taaataatac ttgttgttaa tttctactat agtaataaaa ttgttcggac
    11941 ccagcattaa tttgtctgct acattcatat atcttgattt agcggctatg gttgtctttg
    12001 ttgaaagcca ttttgtacaa tagtaggtca aatacaaaac aattattatt cctataattg
    12061 atataataag tgagattata tctttattaa ttataatcaa cccctatcga ttatactttg
    12121 aacggttttt ataattcttt ccgatttaaa gggtttaact ataaaatcct tagcaccgtt
    12181 tcttatcgct tcgacaacca tggattcttg tcccattgcc gagcacatta ctattacggc
    12241 atttgggtcc attttcctta tttccttcaa tgcatccaat ccatttttat tgggcatggt
    12301 tatatccatt attaccaagt caggtttttc atttttgtat gtttgaatag cctgctctcc
    12361 gtcagcagct tctataatat tttcataacc gctgcttttt aaggtgtcct ttatcatcat
    12421 tctcataaat gcagcatcat ctactattaa aattttacct tccattctat cctccgttgt
    12481 atg
//
LOCUS       DAOPUE010000055        12388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5197_length_12388_cov_10.723344, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000055 DAOPUE010000000
VERSION     DAOPUE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12388
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_12388_cov_10.723344"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2640)
                     /locus_tag="PKH42_06230"
     CDS             complement(<1..2640)
                     /locus_tag="PKH42_06230"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOA20064.1"
                     /translation="MAINIPSKYNPKEFENKIYKIWMDENCFRAEINKDKKPFTIVMP
                     PPNITGQLHMGHALDQTLQDVLIRWKRLMGYEALWLPGSDHASIATEVKVVERLKKEE
                     GKSKEDIGREEFLKRAWKWKEEFGGRIVEQVKRLGNSCDWSRERFTMDDGCNEAVTEF
                     FVRLYERGLIYKGKRIINWCPDCLTTLSDIEVEHEDTAGKYYYVKYPFADNKDEYFII
                     ATTRPETIFGDVAIAAHPEDERYKHLIGRKVIVPLVGRIIPIIADEYPDMEKGTGALK
                     ITPAHDPNDFEIGLRHNLEQISCMNEDGTMNEVAGKYKGMNRFECREAFVKDLEENGY
                     LIKTENTDHNVGTCYRCHNVIEPRISDQWFVKMKPLAGPALKVVENKEMQLVPDRFTK
                     IYNHWLENIRDWCISRQLWWGHRIPAYYCQDCDEMMVQKVAPEKCNKCGSTNIIQDED
                     VLDTWFSSGLWPFSTLGWPHETEDLKYFYPTDVLVTGYDIIFFWVIRMVFSALEVTGQ
                     SPFKYTFIHGLVRDAEGRKMSKSLGNGIDPLDIIDNYGADALRFMLMTGISPGNDTRF
                     NYDRLESSRNFANKLWNASRFVLMNIDGDLTEEERARKNYKTEDKWIISRVNKAAKEA
                     TEYLEKFELGMAAQRVYDFIWNEYCDWYIEIVKPRLYGDDGDDKDTARFVLIRVLKDM
                     LKLLHPFMPFITEEIWSYLPDTSSRLVRANWPEYTEEEISEEAEENIEFIMEAVRSIR
                     NTRAEMNVLPSRKARAIFIPSNTKARLFIESGEKYFSTLANITEVKIVNDKKEIGEDT
                     ASSVIDGTEIYLPLADLIDYEKEIERLEKEKTRLEGELKRVNDKLNNERFMSKAPENV
                     VNEEKEKLQKYQLMMEKVLERLEQ"
     misc_binding    complement(2745..2951)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3103..4275
                     /locus_tag="PKH42_06235"
     CDS             3103..4275
                     /locus_tag="PKH42_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOA20065.1"
                     /translation="MDLREEALKLHKDNKGKIEVISKVRVENAKDLSLAYTPGVAEPC
                     KEIHANPELIYEYTNKGNMVAVVTDGSAVLGLGNIGSRAGMPVMEGKAVLFKSFANVD
                     AFPICIESQDVDMVVQTVKQIAPSFGGINLEDIAAPRCFEIEERLKKELDIPVFHDDQ
                     HGTAVVVLAGIINALKAVGKKIENVTAVVSGAGAAGIAISKLLLSSGINDVIICNSRG
                     ILDKEDERLDPIRKEMALKTNKANKKGALSDALKGADIFIGVSAPNIVTKEMIESMNE
                     NSIAFAMANPIPEILPEVALAGKVKVMGTGRSDFPNQINNVLAFPGIFRGALDVRAKE
                     INEEMKIAAAHAIAELVTEEELNEEYIIPKAFDKRVAKAVARAVSEAAIKTGVNRI"
     regulatory      4365..4551
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4753..5694
                     /locus_tag="PKH42_06240"
     CDS             4753..5694
                     /locus_tag="PKH42_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20066.1"
                     /translation="MKRNSILALTLICIMALLTACAENTEVKSPQPEDINITLPTKDR
                     AGNEISIPNEINRIISISPANTEILIELGFGDKIIAVDEYSKDIEGLPNNIQLFDMMA
                     PDAEQMVALEPDIIYTTGMVMAEGNDPYKPIRDMGVCVAYIPASLSIEGIYEDILFFA
                     KSLGVEKKGIDLVNSMKSKIKEIEEIGKSIENKKTVYFEIAAAPTLYSFGKGVFLNEM
                     IEIIGAENVLSDHDWWISVSEETVIASDPDVIITNVDYIENPAEEIKSRSGWENMKAV
                     KNNDVYYVDNYATSHCNHNIITGLEQMARAIYPDIYQ"
     gene            5691..6716
                     /locus_tag="PKH42_06245"
     CDS             5691..6716
                     /locus_tag="PKH42_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOA20067.1"
                     /translation="MKIKFKIILSIIFSIIVVIMGVGLGSVNVPPSHIISVILNKIFS
                     APLLSDIEGTTAISIIWKLRLPRTLLAFLVGGAVAVSGSVMQSVLKNPLASSYTLGVS
                     SGASLGAALVIVTGFNIPFLGMFTLPVTGLVFGMATVFLAVKFAAMMDKNLETNTIIL
                     VGMVFSLFVNAILTVITAMARDHLAQLTYWQMGSFSLKDWSSVIILFPIVLIGITVLQ
                     NYSKEMDIMTFGEEQAFTMGVDVHKVKWILLSLSAALTGSAIAFVGVIGFIDLIAPHV
                     VRKIFGSNHKTVIPMSVFFGGSFMVLCDLIARTIISPSELPVGAITALIGAPFFAYVY
                     FSRRKGL"
     gene            6713..7522
                     /locus_tag="PKH42_06250"
     CDS             6713..7522
                     /locus_tag="PKH42_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20068.1"
                     /translation="MDAMLTLKNVSAGYNGIDVINNISLCVKEGENLCIIGPNGCGKT
                     TLIKAISGIIPHTGIIKIGGTDINNMKREQVAKKIAVMSQISTIYFSYSIYETVLLGR
                     YLHMKGRTFKEPSAKDREYTDKCLKAVGLFNIKHKQISTLSGGQLQRVYLARTLAQEP
                     NIILLDEPTNHLDLKHQTELIDFLKDWSKYEGHSVIGVMHDLNLAIKLSDNMLVLDNG
                     NIAAYGSTNEIISSNLLNEVYDMNVTEYMVDSLKHWESFGLCHPEPKRRIS"
     gene            7664..8689
                     /locus_tag="PKH42_06255"
     CDS             7664..8689
                     /locus_tag="PKH42_06255"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOA20069.1"
                     /translation="MKKLLVISIVLAVILTACNNIAVPPEEPDNGETVSIEEYYPIKE
                     NTKYSYEGEGNEFATYVAFIDYVSDNRIQNRTNNGGTETVNVYEIKDDQLTLLYSRGE
                     TYFRQNFLNKEYGEGKVLLKGPLEEGNSWAYDENTTATITDLSKEIVTTLGNYQAIEV
                     TLEGEQGKTVNYYAKDVGLIKTISSGEGYEVSSTLSSIENNIPLIQTITLFYPNTDGS
                     IDTIDVQIEFNTNDEPKDIIEEVVKELSVYEILSPNTIINELYFDEEENSVHIDLSDD
                     FITEMNAGSSFENLILQSLTNTLGNYYGVQEVYLTIDGGPYESGHIRIEEGEPLLVDY
                     DNVNTRE"
     gene            complement(8818..9375)
                     /gene="cysE"
                     /locus_tag="PKH42_06260"
     CDS             complement(8818..9375)
                     /gene="cysE"
                     /locus_tag="PKH42_06260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOA20070.1"
                     /translation="MIKFIKSIIEDIDSVFDRDPAARSRMEVLLLYPHIKALIAYKIS
                     HKLYLKRRYFLARLISNIARHRTGIEIHPGATIGKGLFIDHGMGVVIGETAVVGNNVT
                     MFHGATLGGTGNEKGKRHPTVGDNVVIGASAKILGNIYIASGTKVGANAVVLNDTNPN
                     STLVGIPAREVKKGNKIINLSEYAI"
     gene            complement(9393..10277)
                     /gene="cysK"
                     /locus_tag="PKH42_06265"
     CDS             complement(9393..10277)
                     /gene="cysK"
                     /locus_tag="PKH42_06265"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOA20071.1"
                     /translation="MINTLDLIGNTPVIKLPGENIYLKMEKFNPGGSVKDRAALGMIE
                     DAEAKGYLNKNSIIVEPTSGNTGIALALIGRLKGYKVIIVMPETMSMERRNIIEAHGA
                     TLILTEGQKGMSGAIARAHELLKDDNNYFMPDQFNNPANPRIHYDTTGQEIIRQLNDI
                     DIFTAGVGTGGTLTGVSKRLKEYNKNIITAGVEPSKSPVLSGGSPSPHKIQGIGAGFV
                     PGTFNGSFVDEIITITDEEAIDTAVEVSAKIGMLIGISSGANVAAARKLAQKYGSNKK
                     ILTISPDGGEKYLSVVKY"
     misc_binding    complement(10387..10615)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10759..12063)
                     /locus_tag="PKH42_06270"
     CDS             complement(10759..12063)
                     /locus_tag="PKH42_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOA20072.1"
                     /translation="MKQFFKLEENNTNVKTEVLAGITTFMTMAYILIVNPIILSETGM
                     DFGGIFTATALSSLIATLMMALYANYPFALAPGMGLNAFFAFTVVLGPMGKSWEFALT
                     AVLIEGIIFILLSFIKAREAIFESIPLNLKNAVSVGIGIFISFIGLSGAGIVKAGNGT
                     LVALGELTSGAPLLAIIGIIITGLLLIKNVKGALLIGIVITTIIGIPMGVTILPENFS
                     TIVSMPPSLKDVAFKFVGFDQIFSFEMLVVVFTFLFVDIFDTVGTLAGVATKANMLDE
                     EGKLPRVGAALMSDAVGTVVGACLGTSTVTTYVESASGVAEGGRTGMTSLVTAAMFGV
                     ALFFAPLFTLVPSAATAPALVIVGLFMMSPITKINFNDYTEAIPAFLTIVMMPFAYSI
                     AEGIVFGMVSYVILKAISGKFKDISLVMWILSVLFVLRFIFI"
     regulatory      complement(12231..12330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         3905 a         2322 c         2179 g         3982 t
ORIGIN      
        1 ttgctctaat ctttcaagta ctttttccat catcagctga tatttttgaa gtttttcttt
       61 ttcttcatta acaacatttt ccggagcctt gctcataaat ctttcattat ttaacttgtc
      121 gttaactctt ttaagttcgc cttcaagcct tgttttttct ttttcaagtc tttcgatttc
      181 tttttcataa tctattaaat cagctaaggg cagataaatt tctgttccgt caataactga
      241 agaagccgta tcttcgccta tttctttctt atcatttaca atttttactt ccgtaatatt
      301 tgctaaggtc gaaaaatatt tttctcctga ttcaataaat agtcttgctt ttgtatttga
      361 aggaataaat attgcccttg ctttccttga aggcagtaca ttcatttctg cccttgtgtt
      421 acggatactt cgaactgctt ccattataaa ctcaatattt tcttctgcct cttcagaaat
      481 ctcttcttca gtatattcag gccagtttgc tctcaccaat cgtgaagatg tatccggcag
      541 atatgaccat atttcttctg tgataaatgg cataaaggga tgtaaaagct ttaacatatc
      601 ctttaaaacc ctaatcaata caaaccttgc cgtatccttg tcatcaccat catcgccata
      661 aagtctcggt ttaacaattt ctatatacca gtcgcagtat tcattccata taaaatcata
      721 aaccctctgt gcagccattc ccagttcaaa tttttcaaga tattcggtag cttccttagc
      781 tgctttgtta actcttgaaa ttatccattt atcttctgtt ttatagtttt tccttgccct
      841 ttcttcttct gtcaaatctc cgtctatatt cattaataca aatcttgaag cattccacag
      901 tttatttgca aaattacggc ttgattccaa tctgtcataa ttaaatcgtg tgtcgtttcc
      961 gggagaaatc cctgtcataa gcatgaaacg aagagcatct gcaccgtagt tgtcgataat
     1021 atcaagaggg tctatgccgt ttcctaatga tttgctcatt tttctgcctt ccgcatctct
     1081 caccaaacca tgaataaatg tatacttaaa gggtgattga cctgtaactt ccaaagctga
     1141 aaataccatt cttataaccc agaagaatat aatatcgtat cctgtaacca aaacatccgt
     1201 aggatagaag tattttaaat cttctgtttc atggggccag cctaaggttg aaaacggcca
     1261 aagtccggat gaaaaccatg tatcgagaac atcttcatcc tgtataatat tggtgctgcc
     1321 gcatttattg catttttctg gagcaacttt ttgaaccatc atttcatcac agtcctggca
     1381 gtaatatgca ggaatcctgt ggccccacca caattgtctt gaaatacacc agtctcttat
     1441 attttcaagc cagtgattgt aaatttttgt aaagcggtcc ggaaccagct gcatttcttt
     1501 attttcaact acctttaatg caggtcctgc caatggtttc atttttacaa accactggtc
     1561 ggaaattctt ggctcaataa cattgtggca tctgtaacaa gtccctacat tgtggtcggt
     1621 attctctgtc ttaatcaaat acccattttc ttctaggtcc tttacaaatg cttctctgca
     1681 ttcaaaacga ttcatgcctt tgtatttgcc ggcaacttca ttcatggttc cgtcttcatt
     1741 catacagctt atttgctcta ggttatgacg aagtccaatt tcaaagtcgt tagggtcatg
     1801 agccggagtt attttcaatg cacctgtacc cttttccata tcggggtatt catccgctat
     1861 aattggaatt attcttccaa ctagtggaac aattactttc cttccgatta aatgtttata
     1921 tctttcatct tcagggtgtg cagctatggc aacatctccg aaaattgttt ccggtctggt
     1981 tgtagcaatg ataaaatact catccttatt atcagcaaat ggatatttta cataataata
     2041 cttgcctgca gtatcctcat gttcaacttc tatatcggaa agagttgtca ggcagtcagg
     2101 acaccagttt ataattcttt ttcctttata tataagtcct ctttcgtaca gccttacaaa
     2161 aaactcagta acggcttcat tacagccatc atccatggtg aatctttctc ttgaccaatc
     2221 gcaggaatta cctagtcttt taacctgctc aacaattctg ccgccaaatt cttctttcca
     2281 tttccaagct cttttaagaa attcttccct gcctatatct tccttggatt ttccttcttc
     2341 ctttttaagt ctttcaacta cttttacttc ggttgcaatg cttgcatggt cagagccggg
     2401 aagccacaaa gcttcataac ccatcaatct cttccacctg atgagaacat cctgcagagt
     2461 ttggtccaaa gcatgcccca tatgaagctg ccctgtaata ttcggaggcg gcatcacaat
     2521 cgtaaatggt tttttatctt tgtttatctc tgctctaaag caattttcat ccatccagat
     2581 tttataaatt ttattttcaa attccttcgg attgtattta cttggtatgt taattgccat
     2641 aatatccttc ctttctttat attgtatcat tgtcggaata acaagtagta tcattccggg
     2701 gtagagcctg tatgggttat aaataaaaaa ccaccggatt tcatcaaaag gacgagaaac
     2761 cccgcggtac cacctaattt ccattacggc tcttaaaaaa tataacggtt actccgttta
     2821 atcttacttt tacttcagat taaatgctca aaagctacct tcggcacaca ttgacctatg
     2881 aagactttca tccggtggtc ttcactctct gacaagttta tgcacttact cctctttttc
     2941 atcgcaaata tttaattaca tatcatatta ttaaaattta atgtttttgt caacaacttt
     3001 ttcttaattt tgttttttaa atgaatataa aattcaaata atgtataaga cgtaaagtta
     3061 tggtataata attaataatt aaggacgaag gagaaagtaa atatggattt aagagaagaa
     3121 gctttaaaac tacacaaaga taacaaaggt aaaattgaag ttataagtaa ggtcagagta
     3181 gaaaatgcaa aggatttaag cttagcctat acacccggag tagctgagcc gtgcaaggaa
     3241 atccatgcga atcctgaact aatatatgaa tatactaata agggtaatat ggttgcggtt
     3301 gttactgacg gctctgcagt attaggtttg ggtaatatag gttcaagagc cggaatgcct
     3361 gttatggaag gtaaggcagt attatttaaa tcatttgcca atgtagatgc ttttcctatt
     3421 tgcatagaaa gtcaagatgt tgatatggta gttcaaactg ttaaacaaat tgcgccgtcc
     3481 tttggcggta taaatttgga agatattgct gcaccaaggt gctttgaaat agaagaaaga
     3541 ctaaagaaag aattggatat tcccgtattt catgatgatc aacatggtac tgccgtcgtt
     3601 gtattagcag gaataatcaa tgcgttaaaa gcagtaggta aaaaaatcga aaatgtaaca
     3661 gctgttgtaa gtggtgccgg tgcagccggt atagctatat ctaaattgct tctgagttca
     3721 ggaatcaatg atgtaattat atgtaacagc agaggtatat tagataaaga agatgaaagg
     3781 cttgatccga ttagaaaaga gatggcctta aagacaaata aggcaaacaa aaaaggagct
     3841 ctttcggatg ctttaaaagg agcggatata tttataggtg tttctgcacc aaatatagtt
     3901 acaaaagaaa tgattgaaag catgaatgaa aattcaattg cttttgcgat ggcaaatccg
     3961 atacctgaaa tattgcctga agtcgcattg gcaggcaaag tcaaagtaat gggaacagga
     4021 agatctgatt tccccaatca aattaataat gtattagcat tccccggtat cttcagaggt
     4081 gctcttgatg taagggctaa agaaattaat gaagaaatga aaatagcagc agcacatgca
     4141 attgcagaat tggtaaccga agaggaatta aatgaagaat atataattcc aaaagctttc
     4201 gataaaaggg tagccaaagc agtagctagg gctgtttctg aagctgcaat aaaaaccggc
     4261 gtgaatagaa tttaatgaat aagtacttca gtataagtat cgatataatt ttgcatattt
     4321 tcttaattga caatactaat ttaatgtaat atacttatct taattaaaca tctttcgata
     4381 ttggtgcgct taaatgcgat aatagggaat taggttaaaa tcctaaacag tcctgctgcc
     4441 gtgatgagag agcggttttt atgctaatgc aaccactgga tatctccggg aaggttaaaa
     4501 accagcgatg acctctgagt cggaaaacct gccagtatta tttgcgtaga tgccgcaaat
     4561 gctatgcttt acagagtata aaagcgatag cgcaaaaatg gtttatccaa aatatttgtg
     4621 gaatttacgt ttcgggaacg agacgttttt ttatgcccta agccaagact tcgaccggaa
     4681 ggagaaagtc ttgatggtag gtcaaacgca atgcctctct tagatgttta aaaaacaaaa
     4741 atggaggatg aaatgaaaag aaacagtatt ttagctttaa cattaatttg tattatggcc
     4801 ttgcttactg cttgtgccga gaataccgaa gtaaaatctc ctcaaccgga ggatatcaac
     4861 ataacattgc ccacaaaaga cagagcgggc aatgaaatat caataccaaa cgaaatcaac
     4921 agaataattt ccatatcacc tgcaaatacg gaaatactaa ttgaattagg ttttggcgat
     4981 aaaataattg cagtcgatga gtattctaaa gatattgaag gactgcctaa taatatacaa
     5041 ttatttgata tgatggcacc tgatgctgag cagatggttg ccttagagcc ggacataatt
     5101 tatacgaccg gaatggttat ggcagaagga aacgaccctt acaaacctat cagggatatg
     5161 ggtgtttgtg tagcatatat accggccagt ttaagcatag agggaattta tgaagatata
     5221 ttattttttg caaaatccct cggtgttgaa aaaaaaggta ttgaccttgt aaacagcatg
     5281 aaatctaaaa ttaaagaaat tgaagaaatc ggcaaatcta ttgaaaacaa aaagacagtg
     5341 tattttgaaa ttgcagcagc accaacactt tacagttttg gcaagggagt atttttaaat
     5401 gaaatgattg aaataatagg tgccgaaaat gttttatcgg accatgactg gtggatttca
     5461 gtttcagaag aaacagttat tgcatctgac cctgatgtta tcataaccaa tgttgattat
     5521 attgaaaatc ccgcagaaga aatcaaatcc cgctcaggat gggaaaatat gaaggctgtt
     5581 aaaaataatg atgtttatta tgtagacaat tatgcaacct cccactgcaa ccacaacatt
     5641 attacaggct tggagcaaat ggcgagggca atctatccgg acatatatca atgaaaataa
     5701 aatttaaaat aatattatca ataatattca gtattatagt agtaataatg ggagtgggat
     5761 taggaagcgt caacgttccc ccttcccata ttataagtgt aatattaaac aaaatatttt
     5821 cagcaccgct tctttccgat atagaaggta ccactgctat atcaataata tggaagttaa
     5881 gacttccaag aacactcttg gcatttttgg tggggggtgc tgtagctgta agcggctcgg
     5941 taatgcagtc ggttttgaaa aatcctcttg cttcctcata cacattaggg gtatcttcag
     6001 gtgcttcatt gggagcagct ctagtaatag taaccgggtt caacataccc ttcctgggaa
     6061 tgttcacatt gcctgttacc ggcttggtat ttggcatggc gacggtattt ttggctgtta
     6121 aattcgcagc tatgatggat aaaaatttag aaactaatac aataattttg gtaggtatgg
     6181 ttttttcatt gtttgtaaat gccatactga ctgtcataac tgcaatggca agagaccatc
     6241 ttgcacagct gacttattgg caaatgggaa gtttttcatt aaaagactgg tctagtgtaa
     6301 taatactctt tcccatcgtt ttaataggaa ttacagtatt gcaaaattat tcaaaagaaa
     6361 tggatataat gacttttgga gaggagcagg cattcaccat gggagtggat gtccacaaag
     6421 tcaaatggat tcttctcagc ctgtcagcgg cacttacagg cagtgccatt gcatttgtgg
     6481 gagtaatcgg atttattgat ttaattgcac ctcatgtggt aagaaaaata ttcggctcca
     6541 atcacaaaac agtaataccg atgtcagtgt ttttcggagg ctcattcatg gttctatgcg
     6601 acttgattgc cagaacgatt atatcgcctt ctgaattgcc ggtgggagca attacggcat
     6661 taataggtgc accgtttttt gcttatgtat acttttcgag aagaaagggg ttatagatgc
     6721 tatgcttact ttaaaaaatg tatctgcagg ttataacgga attgatgtaa taaataatat
     6781 ttccttatgc gttaaggaag gtgaaaatct ttgcattatc ggtcccaacg gttgcggaaa
     6841 gactacgctt attaaggcaa tttcaggaat tattcctcat acgggaatta ttaagatagg
     6901 cggaactgat attaataata tgaaaaggga gcaagttgca aagaaaatag ctgttatgtc
     6961 ccaaatttca accatttact tttcatacag catttacgaa accgtacttc tcggaagata
     7021 tcttcatatg aagggaagaa catttaaaga gccttccgca aaggatagag aatatacgga
     7081 taaatgttta aaagctgtag gtttatttaa tattaagcat aagcaaataa gcaccttgtc
     7141 gggagggcag cttcaaaggg tttatttggc aagaacctta gctcaagagc caaacattat
     7201 acttcttgat gagccaacaa atcatttgga tttaaaacat cagacagagt taatcgactt
     7261 tttgaaggat tggtcaaaat atgaaggcca ttctgtaatc ggtgtaatgc atgatttgaa
     7321 tttggcaata aaattgtctg ataatatgct ggtattagat aatggaaata ttgctgctta
     7381 cggcagtaca aatgaaatta tttcttctaa tcttttaaat gaagtctatg atatgaatgt
     7441 aacagagtat atggttgatt ccttaaaaca ttgggagagc tttggacttt gtcatcctga
     7501 gcctaagcga aggatttcat aagactcttc tctgtgattg tcatcccgag cctaatcgaa
     7561 ggatttataa taaccgaaac ccggttattt ttttataacg gtaattatta gtgataaatc
     7621 tgggtataat aaattaacaa aattatgtaa agaggagaat ttaatgaaaa agttattagt
     7681 gatttcgata gtgcttgctg ttattcttac agcatgtaac aacattgctg tcccgcctga
     7741 agaacctgat aatggcgaga cagtatcaat agaagaatat tatcctatta aagaaaatac
     7801 aaagtattca tatgaaggtg aaggaaatga atttgcaact tatgtagcat ttattgatta
     7861 tgtttcagac aaccgcattc aaaatagaac aaacaacggt ggaacagaaa cggttaatgt
     7921 gtatgaaata aaggatgatc aattgacttt attatattca agaggagaaa catattttag
     7981 acaaaacttt ttaaacaaag aatatggtga gggaaaagtt cttctgaaag gtcccttaga
     8041 ggaagggaat tcgtgggcat atgatgaaaa cacgactgca accataaccg acttatcaaa
     8101 agaaatagtc actacacttg gcaattatca ggcaattgaa gttaccttgg aaggagagca
     8161 aggaaaaaca gtaaactatt atgcaaagga tgtgggattg attaaaacaa taagctctgg
     8221 tgaaggttat gaggtatctt caactttatc atctatagag aataatatac ccctgataca
     8281 gactattact ttgttttatc ccaatacgga cggctccatt gatacaatag atgttcaaat
     8341 agaattcaac accaatgacg agccaaaaga tattatagag gaagttgtca aggagttatc
     8401 tgtttatgag attttaagcc ccaatacgat aatcaacgaa ttatactttg atgaggaaga
     8461 aaacagcgtt catattgatt tgtccgatga tttcataaca gaaatgaatg cagggtcaag
     8521 ttttgaaaac ctgatacttc aaagtttaac caacacctta ggcaactact acggagttca
     8581 ggaagtatat ttaacaattg acggaggtcc ttacgaatca ggacatataa gaatcgaaga
     8641 aggtgagccc ttattggtag actacgataa tgttaacacg agagaataaa atatggttta
     8701 cataattgaa cattatgtaa accattgggg acacgcatat ccccctcatt acggggacat
     8761 tccttcaata attatctcac aaagaatcac tgtatattga aggtgtgtcc ccatgcatta
     8821 tattgcatat tcgctcaagt taattatttt gttccctttt ttaacctctc ttgcaggaat
     8881 cccgacaaga gtagaattag ggttggtatc attcaatacg acggcatttg ctcctacctt
     8941 ggttcctgaa gcaatataga tgtttccaag tatctttgcc gatgctccta ttactacatt
     9001 atccccgaca gtaggatgtc ttttcccttt ttcatttccg gtacctccca atgtagctcc
     9061 atggaacatt gtcacattgt tccctacaac ggctgtttct cctattacaa cacccatacc
     9121 gtggtctata aaaagtcctt taccgatggt tgcgccggga tgtatttcaa ttcctgtcct
     9181 gtgccttgca atatttgata taagtcttgc caaaaaatat cttcttttta aatataattt
     9241 atgagaaatt ttatatgcta tcaaggcttt aatatgagga tacaaaagta agacttccat
     9301 cctgcttctt gcggcagggt ccctgtcaaa cacagaatct atatcttcaa ttattgattt
     9361 tataaattta atcatgatat cattcctttc aattaatact taactaccga caaatacttt
     9421 tctccgccgt ccggagatat tgtaagtatt tttttattgc tgccgtattt ttgcgctaat
     9481 tttcttgctg ccgcaacatt agctcctgac gaaattccta tgagcattcc aatttttgct
     9541 gaaacttcta cagcagtatc gattgcttcc tcatcggtta ttgttattat ttcatctaca
     9601 aaacttccat tgaatgttcc cggaacaaag cctgccccta ttccctgaat tttatgaggt
     9661 gacgggctgc cgcctgataa tacaggtgac tttgaaggct caactcctgc agtaattatg
     9721 tttttgttat attctttcag ccttttcgaa actccggtta gagttcctcc ggtaccgact
     9781 ccggctgtga atatatcaat atcgtttagc tgcctaatga tttcctggcc tgtagtatca
     9841 tagtgaatcc tggggtttgc aggattatta aactggtcag gcataaaata attattatca
     9901 tctttaagca gctcatgagc ccttgctatt gctccgctca ttcctttttg tccttccgta
     9961 agtatcaatg tggcaccatg tgcttcgatt atgtttcttc tttccatact catagtttca
    10021 ggcataacga ttattacttt atatcctttc aatcttccta ttagagccag tgcaatacct
    10081 gtatttccgc tggtaggctc cacaatgatg ctgtttttat ttaaatatcc ctttgcttca
    10141 gcatcttcaa tcatccctaa tgcggctctg tccttaacac tgccgccggg attgaatttt
    10201 tccattttta aataaatatt ttctcccggt aatttaatca caggagtgtt tcctattaaa
    10261 tctaatgtat taatcatatt attacctctt tcttttccac taaatcgata atcaatatag
    10321 ctacaaacgg aatatatatg cataagtggt attcgcaggt taggacatac aaaaaatccg
    10381 cctctgcaaa gagacggatt gaaatccgcg gttccactct aattggctct atagccccct
    10441 tatatagcac catcatgcta ctccactata acggatggtt ccgcagcagc ctacttaatt
    10501 cggtgctgta ctccaaaggg cacttcacat atacttccaa taagcatcct tccagccttg
    10561 agatgctctc tctgtaaatt cgttatatgc tactttcctt tttcgccgta tttcctacta
    10621 atttagttgg gtatattttc tttattatat tatactgctt tataaaatgc aacacttttt
    10681 ctaaaaattt ttttaatcta tttaaaaata aaaaaacctg ccgtttatct ccaacggcag
    10741 gctgttttat cttttacttt aaataaatat aaatcttaaa acaaatagta ccgataaaat
    10801 ccacataacc aaagatatat ctttgaattt gccggatata gctttcaaaa taacatatga
    10861 taccattcca aatacaattc cttccgcaat actatatgca aaaggcatca taacaattgt
    10921 taagaaagcc ggtattgctt ctgtataatc gttgaaattt attttggtta tagggctcat
    10981 cataaacaaa cctacaataa ccaatgcagg tgcagttgca gctgatggaa ccaatgtgaa
    11041 caatggagcg aaaaacagtg caactccaaa cattgctgca gtaacaagcg atgtcattcc
    11101 tgttcttccg ccttcagcaa cgcctgaagc actttcaaca tatgttgtaa cagtacttgt
    11161 tcctaaacat gcaccaacaa ccgtaccaac agcatcagac attagagcag ctcctactct
    11221 cggcaatttc ccttcttcat ccagcatatt tgcctttgtg gcaacgcctg ccaatgttcc
    11281 gacagtatca aaaatgtcta caaagaggaa tgtgaatact accaccagca tttcaaaaga
    11341 aaatatttgg tcaaatccta caaatttaaa tgcaacgtcc ttaagtgaag gaggcatgct
    11401 tacaattgta ctgaaattct ccggcagtat tgtaactccc atcggaattc caataattgt
    11461 tgttataact attcctatta acaatgctcc cttaacgttt tttatcagca ataatcctgt
    11521 aataatgatt cctataatag ccaataatgg agcacctgat gtcaattcgc ccagagcaac
    11581 aagcgtaccg ttgcctgctt ttactattcc tgcaccggat aaacctataa aagatataaa
    11641 tatacctatt ccaacggaaa cagcattctt taaatttaaa ggtatagatt caaatattgc
    11701 ttctctggct tttataaagg atagtaaaat aaatataatt ccctcaatta atacagcagt
    11761 tagagcaaat tcccatgact tgcccattgg tcccaataca acagtgaatg caaagaatgc
    11821 attaagtccc ataccggggg ctaaggcaaa cggatagttg gcatagaggg ccatcataag
    11881 tgttgcaata agtgatgata aggccgtagc agtaaatata ccgccaaaat ccattccggt
    11941 ttctgataga atgataggat taacaattaa aatataggcc attgtcataa aagtagtaat
    12001 gcctgctaat acttctgttt ttacattagt attattttcc tcaagcttaa aaaattgttt
    12061 caaagtatta ttcctcctga ttttttaata ttttatcata tgaattaacc acaaaaaata
    12121 aataaagcct gtcgaggtag gaagtgaaac ctacccgact gggcttttat cccttcggtg
    12181 taatacttaa agtatctgta ccgctcggtc cagcaagcaa tcttcggaac cctagataca
    12241 ctttcactca tagccaaatg attaggtcat tttgtagaaa catctcaacc atataatgag
    12301 atatatatga gctatattta ttttcatatt gataatacca atatgtttta aatatgtcaa
    12361 taggaaaatg atgtatactt aaaacgct
//
LOCUS       DAOPUE010000056        11695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5614_length_11695_cov_10.585567, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000056 DAOPUE010000000
VERSION     DAOPUE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11695
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_11695_cov_10.585567"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(172..255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            555..956
                     /locus_tag="PKH42_06275"
     CDS             555..956
                     /locus_tag="PKH42_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOA20073.1"
                     /translation="MGKKIRVKPSKAQSTAAFFVGIVFCLIGLFVAIPMAGPFGIFWT
                     LVAVIITVMHGKNAFTEEGVSTHEIIIEDNDFIKKTKLINNTAEETENEDYVNISDSK
                     KRLEEIKLLYENGLISKDEYEEKRKSIIDNI"
     gene            complement(1022..2407)
                     /locus_tag="PKH42_06280"
     CDS             complement(1022..2407)
                     /locus_tag="PKH42_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOA20074.1"
                     /translation="MDVKYVAEPFRIKMVEPLKMLSREEREQKIKEANYNVFNLKAED
                     VYIDLLTDSGTNAMSSYQWAGVMLGDESYAGGTSYYKLIEAGQDIFGYKIIQPVHQGR
                     AAEKVFFPILLSKGKYAIANMFFDTTRAHVEIAGARAIDCVVEEAKDPTLRAPFKGNM
                     DVEKLEKLINELGRENVGVVVMTITNNSAGGQPVSVQNMRDVSAVCKKYNIPLDIDAA
                     RFAENAYFVKQREEEFKDWSIKDITREMFSYADFFTMSAKKDAIINMGGMIGIKEDNQ
                     EFYQKVKANCISFEGFTTYGGLSGRDLEAMAIGLYEGLDENFLRYRIGQLEYLAAKLD
                     KAGIAYQAPVGGHGLFIDAKAMFPHIPYYEFPAQALAIELYKEAGIRSCDIGSYMLGN
                     DPDTGEQLKSEFEFTRLAVPRRVYTQAHFDIMAEALTAIKERAASVKGYRITWEPKIL
                     RHFQASLEPIE"
     gene            complement(2556..3518)
                     /locus_tag="PKH42_06285"
     CDS             complement(2556..3518)
                     /locus_tag="PKH42_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOA20075.1"
                     /translation="MRRILITGALGQIGSELTEEMRKIYGSDNVVASSRRIKEGHEKL
                     IESGPFEIINVNDPKTLASAVDKYKINTIVNLAAVLSAVGEKEPQQCWNINMNGLYNI
                     LEIAKEKNCMVYTPSSIAAFGPSTPKDNTPQDTIQRPTTMYGVTKVAGELLCDYYYKR
                     FGVDTRGVRFPGLISYKTLPGGGTTDYAVHIYYEALKNKKYTSFINKGTYMDMMYMPD
                     ALRAIIDLIEANPDRLKHRNAFNVSAMSFEPEMLAESIKKFIPEFKLEYDVDPVRQEI
                     AESWPNSLDDSDAREEWDWNPQYDLEAMTKDMLEKLRIKLNIQE"
     gene            3837..4952
                     /locus_tag="PKH42_06290"
     CDS             3837..4952
                     /locus_tag="PKH42_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOA20076.1"
                     /translation="MNRKKYELAVQLRHELHQHPELSNNEARTKEHLIKFLKEHTKLE
                     IVDRGRWFYAVYKSAHGKKNIAFRADFDAVPIDEYIDIPHGSKIKGVSHKCGHDGHSA
                     SLAGFALEIDRYGADNNVYFLFQHAEETGDGAKEAAVFITENNIDEIYAYHNMSGIPF
                     KAVAVKNGVSFCASKGMIIHMEGIPSHASMPEKGRNPSFAIANIINSVPEFTSPDNYK
                     GMVLCTVIQVAIGERAFGTSASKGELLLTIRALYEEEMNMLQKKIEELAKNEAKKYGL
                     KVNFEYVDVFPETYNHGENVDKIRKAAKNIGYELIEMKEAIRSSEDFGYYTKSTKGAI
                     FLIGNGEDYPAVHINEFDFRDELIEVAADLFKEILLH"
     gene            5021..5368
                     /locus_tag="PKH42_06295"
     CDS             5021..5368
                     /locus_tag="PKH42_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HOA20077.1"
                     /translation="MNKRFISLIVLLILAIVIVAVINHFILNNGETINKVSFNATVLE
                     NYGNSILVNPLEGSPELNSSDKIVVRVPVDNAALKDLSEFTVGSKVKITYDGMIMESY
                     PAQINAYNVEFAK"
     gene            5428..5979
                     /locus_tag="PKH42_06300"
     CDS             5428..5979
                     /locus_tag="PKH42_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA20078.1"
                     /translation="MIFNEKTVTLKNGKVCVLRSPKASDAEEMLEYLKKTAAETDFLL
                     RYPEEVVMTIEEEIDFINFVRDNPRGIMICAFIDDKLVGTTHLMGLGDRFKISHRASF
                     GIAVLKEAWHLGIGNALITEMLKIAEDTGFELVELEVASTNHNAIELYKKFGFKIYGT
                     RENGFKFKDGTYCDEHLMLRRIK"
     gene            6073..7011
                     /locus_tag="PKH42_06305"
     CDS             6073..7011
                     /locus_tag="PKH42_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20079.1"
                     /translation="MYSELNKKLEEVQQKVYRLNKIDSILAELRNDAAELRQKVYELK
                     AILDKENLDVEKLDNTNVVSIFYSIIGKLEDKKEKEQREALSARLKYEQASYDLLSIE
                     AEISKLTQEQECYKGCINEFNSLYEQKKELLIKSCEASANKILELTNKINESKRNLKE
                     LKEAISAGQSAVYSLDSALGSLNSAQGWGTWDLLGGGLIADLAKHSHIDNAMREAEQA
                     HNELRRFKSELADVRINYDISFEMDGFAKFADFFFDGLIADWYMQSKINSSYESVSNV
                     KNQVTSTIYKLQQMESLEKQYLEKLENELKNIIIIS"
     gene            7029..7262
                     /locus_tag="PKH42_06310"
     CDS             7029..7262
                     /locus_tag="PKH42_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOA20080.1"
                     /translation="MDGGNNKDKPPKKYNEVFRALADFTQIGVSIAASVLIGVLLGQY
                     LDDKFGTEPWFLSVFSILGVALGIRTLFNIAKK"
     gene            complement(7379..7969)
                     /locus_tag="PKH42_06315"
     CDS             complement(7379..7969)
                     /locus_tag="PKH42_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOA20081.1"
                     /translation="MNNHLLFKQNQQLAGAVNINSNLMNYTVNVPISLMKSIEGKYFV
                     GTAPNLRFGNATNAWARLYNPPNSGVNLFVNTWTVSDIFTTPYSVQIWFNSTPPGFIQ
                     FSQSVTPSNLTIIPQPQSHIQLQYAISVSGLPTGGIKAYGRYGLAGTTINSEEEGKFI
                     FPPGGSYLIFLSNPERPTLLATGNIAFGWWEEPIMQ"
     gene            8125..8883
                     /locus_tag="PKH42_06320"
     CDS             8125..8883
                     /locus_tag="PKH42_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOA20082.1"
                     /translation="MDNKNYKERIESNRTFMKSAFTEKGYKSDQQKQLPQPPLAKQSV
                     NENKIKLTKDFSSVIKEANILTIINERKSHRVFKNDSITLEQLSFLLWVTQGVKGIRG
                     DNYATERTVPSGGARHPFEVYIAVINVEGLEKGIYHYLSLSHELELIRLLENIENSVV
                     ESLDNQKWAGKSAAVFYYTAIPYRSEWRYTISAHKLVLLDAGHAVQNLYIGCGAIGCG
                     TCAAADYNQELADKLLQVDGEDEFVVYIAPLGLI"
     gene            8916..9434
                     /locus_tag="PKH42_06325"
     CDS             8916..9434
                     /locus_tag="PKH42_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HOA20083.1"
                     /translation="MKITIIGYSSSGKSTLAAKLGNIYNCPVLYLDRVHFKENWIERD
                     DETAKAMVKEFMDHNDNWVIDGNYTNLLREQRLKEADMIIFMCFSRLDCLRQAINRYK
                     TYKGKTRESMADNCSEKLDIEFIKWILLDGRTKEKKEQYAETIRKYNQKIVILRNKKE
                     TASFLEKITHRK"
     gene            9672..10751
                     /locus_tag="PKH42_06330"
     CDS             9672..10751
                     /locus_tag="PKH42_06330"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA20084.1"
                     /translation="MADILILTASFGMGHKSVSNALKEQIEAEYKHVKVSIEDVLEIV
                     NPKVKKFSSKIYSELTESYPSIYNKLYDVKAKNKNNIIDNVLSSLYYKRFYEYMEIEK
                     PKVIISTFPLCSCIVAKVKEEYNDDVNLITVITDVVDSWEWIYEGTNKYLVPTDEIKD
                     RLIAKGVNEENILVTGIPVKKEFLDKKICFSTDKKNTILVVLSGIDSVSKKLLKKLDE
                     YTEYKIKIVTGRNEKLYGKLSDMEYSNLEIYGYVNNLDEMMDESVFIVTKPGGVTVFE
                     AINKEVPLIVLNSIIGQEIGNIEFIKKNKIGIVINDLDDLLYILDYNINNPDTSDKYV
                     KNIKRIKKTLNYSGLSIDSLFDGVI"
     gene            10962..>11695
                     /locus_tag="PKH42_06335"
     CDS             10962..>11695
                     /locus_tag="PKH42_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20085.1"
                     /translation="MKKIMFLSVVILTLSFVTAGCSAIKEPLDISTSTNKEPPDISIF
                     IDNKEIEYVCAKNKWDGSVFDREDTFVTIFKEQKDIPIFINGSIAEISFKNNPPDAFQ
                     IMDILIDEESRQIYTDKEIINIPVELINGKCSFKIEKHLASSLSSYYEPGKKDIRGFR
                     MIASWGENECEYAFIIKTYEIDRTEYLTGEIITDGDYEILSSGIGCICFVPDKESREI
                     IEDKYSSYDLYYLNDESYFLYYDDISV"
BASE COUNT         4075 a         1774 c         2183 g         3663 t
ORIGIN      
        1 atttttttta tttttgataa agtcaggatg tcaatttata tccgacgctt atccctcctt
       61 tctcatgcat aaaaatttct atccggttga tgtcaaaata aaaactgtat gccaaggcat
      121 acagtttaag taccaaaata cactaataca taatgcatta gttcatcact gttcggcaac
      181 ctggcaatca tcgcatttca cattagaccc atggctttgc gtccttgcct ttcgacaagt
      241 ttgctattat cgtaggataa tattaaattt ttctatttgt gcatttattt tactatattt
      301 gatataatta tgaaagattt tttttaaaaa taaatagaat tttgttgaat ttctttcgac
      361 taatttcgat ttatttcgta gtattaactg aagaattgtt aaaactctat ttcttctcta
      421 tgcttatgat gacagattaa ttgggcgtaa actgtaattg ctcgatgtag cgataatatg
      481 aaaggtaaaa tttattgtaa atgtagatga ttgttgatat aattaacaaa gatattaatt
      541 attaaggagt gtttatgggc aaaaaaatta gagttaagcc aagtaaggct caatcaactg
      601 ccgcattttt tgttggaatt gtattttgtt taataggttt atttgttgca ataccgatgg
      661 caggtccatt tggtatattc tggaccctgg tcgcagttat cattacggtt atgcatggca
      721 aaaatgcttt tactgaagag ggagtttcaa ctcatgaaat aataatagaa gataatgatt
      781 ttattaaaaa aactaaatta attaataata ccgctgaaga aacagaaaat gaagattatg
      841 taaatatttc agattctaaa aaaagactgg aagaaataaa gcttttgtat gaaaatggtt
      901 taataagcaa agatgagtat gaagaaaaac gaaaaagtat tatcgacaat atttagtata
      961 attcataatt tcggggacag accttaataa agtctgtccc cggtattaga atgatattgt
     1021 gctattcgat aggctccagg cttgcttgga aatgacgaag tattttaggt tcccaagtaa
     1081 tcctgtatcc cttaacactt gctgctcttt cctttattgc tgttaaagct tctgccatta
     1141 tatcgaaatg agcttgagta tatactcttc ttggaactgc cagccttgta aattcaaatt
     1201 cagactttaa ctgttctccc gtatcaggat cgttgcctaa catatatgaa ccgatatcgc
     1261 aggatctgat tcctgcttct ttgtataatt caattgctaa agcttgtgcc gggaattcat
     1321 aataaggaat atgcgggaac atagcctttg catctatgaa taatccgtgc ccgccaaccg
     1381 gtgcttgata tgctatacct gctttatcta attttgctgc taagtattcc aattgtccta
     1441 tcctatatcg taagaaattt tcatcaagac cttcgtatag accaattgcc attgcttcca
     1501 aatctctgcc ggataatccg ccgtatgttg taaagccttc aaatgaaata cagttggctt
     1561 ttactttttg ataaaattct tgattgtctt ccttgattcc tatcataccg cccatattaa
     1621 taattgcatc tttcttagca ctcattgtaa agaaatccgc atagctgaac atttctcttg
     1681 ttatatcttt tattgaccag tctttgaatt cttcttccct ttgcttaaca aagtatgcat
     1741 tttcagcgaa tctggcagca tcgatatcta atggaatatt atatttctta catacagccg
     1801 atacatctct catgttctga actgatacag gctgtccgcc ggctgagtta ttggttatag
     1861 tcattacaac tacacctaca ttttctcttc caagctcgtt aataagtttt tctaactttt
     1921 caacatccat atttccttta aagggagctc ttaaagtagg atccttagct tcttccacta
     1981 cacaatcaat tgctcttgca ccggcaattt ccacatgagc tctggttgta tcaaagaaca
     2041 tatttgcaat ggcgtattta cctttggata acaaaatcgg gaaaaatact ttttctgcag
     2101 cacggccttg atgaaccggc tgaatgattt tgtatccgaa tatgtcctga ccggcttcaa
     2161 taagcttgta ataactagtt ccgccggcat atgattcatc tccgagcatt actcctgccc
     2221 attggtacga gctcatagca tttgtaccgc tgtctgtcaa taaatcgatg taaacgtctt
     2281 ccgctttaag attgaacaca ttataatttg cttctttaat cttttgctcc ctctcttcgc
     2341 gagataacat tttgagtggt tctaccatct taattctaaa cggttctgct acatacttaa
     2401 catccatatt gttttctcct tttcaattgt tagaaaattc ttaattacaa tttatcattt
     2461 taataaaatt tttgcaatat aaaattgagt taattcacgg taagcattca tcaaaaattt
     2521 gggacattcc ttgaaggaat gtcccaaagt ataaattatt cttgtatatt taatttaatt
     2581 cttaattttt caagcatatc ttttgtcatt gcttctaagt catattgcgg attccaatcc
     2641 cattcttctc tcgcatcgga atcatctaag gaattaggcc atgattcagc tatttcctgc
     2701 cttaccggat caacatcata ttcaagctta aattcaggta taaatttttt tatagattca
     2761 gccaacattt caggttcaaa actcatggct gaaacgttaa aagcattgcg gtgtttaagt
     2821 ctgtccggat ttgcttctat taagtcaatt attgctctta gtgcatccgg catatacatc
     2881 atatccatat atgttccttt attgataaag cttgtgtatt ttttattctt caaagcttca
     2941 tagtaaatat gtactgcgta atcagttgtt cctccccccg gtaacgtctt gtatgaaata
     3001 agtcccggga aacgaacacc tctagtgtct acaccgaatc ttttgtaata atagtcacat
     3061 aataattcac cggcaacttt agttactcca tacatggttg tgggtctttg aattgtatct
     3121 tggggagtat tgtcctttgg ggttgaagga ccaaaagctg caatggaaga tggtgtgtaa
     3181 accatgcaat ttttttcttt ggcaatttct aagatgttgt acagaccgtt catattaata
     3241 ttccaacatt gttgaggttc tttttctcca acggcagaca acacggctgc aaggtttaca
     3301 atggtgttta ttttatattt atctaccgct gatgccaatg ttttcgggtc attgacattt
     3361 atgatttcaa atggacctga ttcaattagt ttctcatgtc cttcctttat tcttctgctg
     3421 ctggctacta cattgtctga accgtaaatc tttcgcattt cttcagttaa ttcggagcct
     3481 atttgaccca atgcccctgt tattaaaatc cttctcaatt aattacctcc cataaatata
     3541 ctatgatact atataattat gtatatataa taacataaaa cttataaaat ataaacaaaa
     3601 gttttctaaa aaatactatt ttattcaaca aaaaataaga attgtctcca aatagaggac
     3661 gattatctga ttgattataa aaggttaata tttataattg atattgaatt gagaaaatgt
     3721 tattatctaa tagtcaaaat gttatgaata ggaattagag gaatttcaag gtacgcccgc
     3781 ggaatcgccg aatttaggcg gagttaaggt ctaccccaaa attccatagg agagacatga
     3841 ataggaaaaa gtatgagtta gcagtgcaat tacgacatga attacatcaa catcctgagc
     3901 tttccaataa tgaagcccgg acaaaagaac atttgataaa gtttttgaaa gaacatacaa
     3961 aattagaaat agtggacaga ggcagatggt tttatgctgt atataaatca gcccacggaa
     4021 agaaaaatat agcatttcgt gcagattttg atgcagtccc gatagatgaa tatatagata
     4081 ttccacacgg gtctaaaatt aaaggtgttt cgcataaatg cggacatgac gggcattctg
     4141 cttcattagc aggttttgca ctggaaattg accgatacgg ggccgacaat aatgtctact
     4201 ttttatttca gcatgctgaa gaaaccgggg atggagcaaa agaagcagcc gtatttataa
     4261 ccgaaaataa tattgatgaa atatatgctt atcacaatat gagcggcata ccttttaaag
     4321 ctgttgccgt gaaaaacgga gttagttttt gtgcctcaaa gggcatgata atacatatgg
     4381 aaggtattcc ttcacatgcc agcatgccgg agaaggggag aaatccatcc tttgctattg
     4441 caaatataat aaattcagtt ccggaattta cttctcccga taattataaa ggaatggtcc
     4501 tttgtacagt aatacaagta gctatagggg agagagcttt tggtacaagt gcaagcaagg
     4561 gtgagctctt gctgactatt cgcgctttat atgaagaaga aatgaatatg ctgcagaaga
     4621 aaattgagga actggcgaag aatgaagcta aaaagtatgg actaaaggta aactttgaat
     4681 acgttgatgt tttccccgaa acatataatc atggtgaaaa tgtggacaaa ataaggaagg
     4741 cagcaaaaaa tataggatat gaattgatag aaatgaagga agcaatcaga agctcggaag
     4801 atttcgggta ttacacaaaa tcaaccaaag gtgccatctt tttaataggt aatggagaag
     4861 attaccctgc tgttcacatt aacgaatttg atttcaggga tgaactgatt gaagttgctg
     4921 cagacttatt taaggaaata ttgttgcatt aaaggaacgt aagtgagtat aattggaaca
     4981 tttctttatt aataacgtct atatacttga ggtgataaaa atgaacaaaa ggtttatatc
     5041 tttgatagta ttattaattt tggcaatcgt aatagttgct gttataaacc attttatatt
     5101 aaataacggt gaaacaatta acaaagtatc ttttaatgca actgtacttg aaaattacgg
     5161 caattcaata ctggtaaatc cattagaagg ctcaccggag cttaattcaa gcgataaaat
     5221 tgttgttagg gttcctgtag ataatgctgc tttaaaagat ttatcggaat tcaccgttgg
     5281 cagcaaagta aaaattactt atgacggaat gattatggaa agctatcctg cacaaataaa
     5341 tgcctataac gtcgagtttg caaaataaaa ttatatcatc ctccccatta tgtggaggaa
     5401 atcgattaaa ttcgaaggtg gttaattatg atattcaatg agaaaactgt tacattaaaa
     5461 aatgggaaag tatgtgtatt aagaagtcca aaagcatctg atgcagagga aatgcttgag
     5521 tatttaaaga agacggcagc tgagacagac tttttgttgc gttatcctga agaagtagta
     5581 atgacaattg aagaagaaat agattttata aattttgtaa gagataatcc aaggggtata
     5641 atgatttgtg cttttattga cgataagctt gtcggaacaa cacacttaat gggtcttggt
     5701 gacaggttta aaatctctca cagagcatct ttcggtatag ctgttttgaa ggaagcatgg
     5761 catttaggaa taggaaatgc actaataact gaaatgttaa agatagcaga ggataccggg
     5821 ttcgaactgg ttgagttaga ggttgcaagc acaaatcaca atgcaataga gctatataaa
     5881 aaatttggat ttaaaatcta cggcacaaga gaaaacggtt ttaaatttaa agacgggaca
     5941 tattgtgatg agcatttgat gctgagaagg ataaaataac cgtgtcattc tgaacgtatg
     6001 tgaagaactc atacattaaa gatagaatcc ttctctatgc tcaggatgat aataaattat
     6061 ctgggagata ctatgtattc agaattaaac aagaagttag aagaagtcca acaaaaagta
     6121 tacagattaa ataaaatcga ttcaattctt gccgaattaa gaaatgatgc ggctgaatta
     6181 agacaaaaag tatatgagct gaaggcaata ttggataagg aaaaccttga tgtagagaaa
     6241 ttggataata caaatgtagt atctatattt tactctatta tcggtaaatt ggaagataaa
     6301 aaagaaaagg aacaaagaga agcactgtcg gcaagactta aatacgagca agcaagttat
     6361 gatttgttaa gtatagaagc agaaatttct aaattaacac aagagcaaga gtgctacaaa
     6421 ggctgtatca atgaattcaa tagtctgtat gaacaaaaaa aagaattact gatcaaatcc
     6481 tgtgaagctt cagctaataa aatattagag ttaacaaata aaataaatga gtcaaaaaga
     6541 aatttaaaag agcttaagga ggcaatttca gcaggacaat ccgcagtata cagcctcgat
     6601 tcagccttgg gcagtctaaa ttctgctcag ggttggggaa catgggactt gttagggggc
     6661 ggacttattg cagacttggc aaaacattct catattgata atgcaatgag agaagccgaa
     6721 caagctcata atgaattgag aagatttaaa tctgaacttg cagatgtaag aatcaactat
     6781 gatataagtt ttgaaatgga tggttttgcc aaatttgcgg atttcttttt tgacggactc
     6841 attgcagact ggtatatgca atctaaaatc aatagttcct atgaaagtgt atcgaatgtt
     6901 aaaaatcagg ttacaagcac catatataag ctgcagcaga tggaaagcct ggaaaaacaa
     6961 tatttagaaa aattagaaaa tgagctgaag aatataatta tcatttcata gaaatggagg
     7021 ggttatcttt ggatggggga aacaataagg ataaaccacc aaagaaatac aatgaagttt
     7081 ttcgtgcttt agctgacttt acgcagatag gagtgtctat agcagcatca gtgttaatcg
     7141 gcgtattgtt aggacagtat ttagacgata aatttggtac tgaaccatgg tttctttcgg
     7201 ttttttctat attgggtgtg gcgcttggaa tcaggacctt attcaatata gctaaaaaat
     7261 aaaaatgata aaaaagagaa ttctaaaatt ctggggacgg ttcttttcgt tctcagaatt
     7321 tttatgacaa ggggacgggg gtactgtcat caaatttttt gctgttatgt ttagacaact
     7381 attgcataat tggttcctcc caccatccga atgcaatatt tcctgtagcg agcagggttg
     7441 gtctctcggg gtttgataaa aaaatcaaat atgaaccgcc gggaggaaat ataaattttc
     7501 cttcttcctc tgaatttatg gtagtaccgg caagaccgta tctgccgtaa gccttgattc
     7561 cgcctgttgg caaaccactg accgaaatag catattggag ctgtatatga gactgcggct
     7621 gtggaataat tgtcaaattg gatggagtaa cagattgaga aaattgtata aatcctggag
     7681 gtgtactgtt aaaccaaatt tgtacactgt atggagttgt aaagatgtca cttacagtcc
     7741 aagtatttac aaataaattt acacccgaat tcggcgggtt gtacagcctt gcccatgcat
     7801 tggtagcatt accgaatcgc aggtttggag ccgtcccgac aaaatatttt ccctcaatag
     7861 atttcattaa gcttatggga acattaacag tgtaattcat taaattcgaa ttaatattta
     7921 cagctcctgc aagctgttga ttttgtttaa ataatagatg gttattcatt agatttcaac
     7981 ctttcatatt atagttattt attatatgat ataatatatg aaaggtgaat ttaccatgtc
     8041 tatctgaact ttacaaacgt aatctttaat aataaaataa tttagaaagg ggaaaaatgg
     8101 agcaatccat tagataatgg tattatggat aacaaaaatt ataaagagag aatagaaagc
     8161 aacagaacat tcatgaaaag tgcatttacg gaaaaaggat ataaatcaga ccaacaaaag
     8221 cagctgcctc agccgccttt agctaaacaa tcagtcaatg aaaacaaaat aaaacttaca
     8281 aaagactttt caagtgtaat taaagaagca aatattttaa cgattatcaa tgaaagaaaa
     8341 agccaccgtg tttttaaaaa cgatagtatt acattagagc agctgtcatt cttattatgg
     8401 gttactcaag gcgtaaaggg aataagaggc gataattatg ctacggaaag aactgttccg
     8461 tcaggaggtg ccagacatcc atttgaagtt tacatagcag ttataaatgt agaaggttta
     8521 gaaaaaggaa tttatcatta tctgtcgcta agccatgaat tagagttaat caggttatta
     8581 gaaaatatag aaaactctgt agtcgaaagt ctcgacaatc aaaaatgggc agggaaatcg
     8641 gcagctgtat tctattatac tgccatacca tatagaagtg agtggaggta tacaatcagc
     8701 gcgcataaat tagtattatt ggatgccggt catgcagttc aaaacttata tattggatgc
     8761 ggagcaatag gatgcggaac ttgtgccgca gcagattata atcaggaatt agccgataaa
     8821 ttactgcaag tggacggcga ggatgaattt gtagtatata ttgctcctct tggacttata
     8881 taagagacat caaacaataa atacgaggtg ttttcatgaa gattacaata attggttaca
     8941 gcagttcagg caagtccacc ctggcagcta agctggggaa tatttataat tgtccggttt
     9001 tatatttgga tagagtacac tttaaagaaa attggataga acgggatgat gaaacggcta
     9061 aggccatggt gaaggaattt atggaccaca acgacaactg ggtcattgac ggcaattata
     9121 ctaatcttct tagagagcaa agattaaaag aagcagatat gataatattt atgtgttttt
     9181 caaggctgga ttgcttgagg caggcaatta acagatacaa aacatataag ggaaaaactc
     9241 gtgaaagcat ggctgataat tgttctgaaa aattggatat cgagttcatt aaatggattt
     9301 tactggatgg aagaacaaaa gaaaagaaag aacagtatgc tgaaacaatc cgtaaatata
     9361 atcaaaaaat tgtgatatta agaaacaaaa aagaaaccgc ttcttttttg gagaaaatta
     9421 cgcataggaa atgaaatatt attatgtgtt aatgataaaa agctcttcga ggtgtatttt
     9481 gagccgaacg aaccgcaaaa atttatgtat tatgataatt ttttataaaa aggtagatcc
     9541 aaatttacct ttttttaatg aaaaattaat aatattctat attataatta atatataaat
     9601 ctaatactaa ctatatcatt atatgtttat gtatttagaa ttcaattaca tatgaagaaa
     9661 ggagcattta tatggctgac attttgatat tgaccgcatc ttttggaatg gggcataaaa
     9721 gtgtttccaa tgccttaaaa gaacaaattg aagctgaata taagcatgtg aaggtgagta
     9781 ttgaagatgt attggaaata gtaaacccca aggttaaaaa atttagttca aaaatatact
     9841 cagaattgac agaaagctat ccatcaattt ataacaagct gtatgacgta aaggcaaaaa
     9901 ataagaacaa tataatagac aatgtgctga gcagcttata ttataaaagg ttttatgaat
     9961 atatggaaat tgaaaaaccg aaggttataa tcagcacttt tcccttgtgt tcatgcattg
    10021 ttgccaaagt taaggaagaa tataacgatg atgtaaattt aattacggtt ataaccgatg
    10081 tcgtggacag ctgggagtgg atttatgagg gaacaaacaa atatttggtt cccaccgacg
    10141 agataaaaga caggttaatt gccaaaggag tcaatgagga aaatatactt gtaacaggca
    10201 tacctgttaa aaaagaattt ttggataaga aaatatgttt ttcaactgac aaaaagaaca
    10261 caattcttgt tgtgttaagt ggaattgaca gtgtttcaaa aaagctctta aaaaaacttg
    10321 atgaatacac agaatataaa atcaaaatcg ttacgggaag aaacgagaag ctatatggaa
    10381 aattatccga catggagtat tctaaccttg aaatttacgg atatgtgaat aatttggatg
    10441 aaatgatgga tgaatcggtt tttattgtta caaagcccgg aggcgtaaca gtatttgaag
    10501 caattaataa ggaagtgccc ttgatagtac tgaattcaat catcggccag gaaatcggaa
    10561 atattgaatt tatcaagaaa aacaaaattg gtattgtaat aaacgacctt gatgatctgc
    10621 tctatatact ggattataat atcaataacc cggatacttc cgataaatac gtaaaaaata
    10681 taaaaagaat caaaaagact ttgaattaca gcggtctttc aattgattcc ttgttcgacg
    10741 gggtaattta agtgaaagat accctactgc ttaacatata aataaagaag atggtgtatc
    10801 cttttggttt aaatttaaat aaagttaaaa ctttcagcac agcttaatga acaaaaccaa
    10861 tataataaac gtctaaaata tatacactat ttaccaaaag caggagtcca tattacgttc
    10921 ttgaagaaag cgttctcggt tttaatgtaa ggggggatat aatgaaaaaa atcatgtttt
    10981 tatcagttgt gattttaaca ttgtcatttg ttacagcagg gtgttcagct ataaaggagc
    11041 cattagacat atcaacttca accaataaag aaccgcctga catttcaata tttatcgaca
    11101 acaaagagat agaatatgtt tgtgcaaaaa ataaatggga tggaagtgtt tttgacagag
    11161 aggatacttt tgttaccata tttaaagaac aaaaagatat tcctattttt ataaacggca
    11221 gcattgctga aatttcattt aaaaacaatc cgccggacgc atttcaaatt atggatattt
    11281 taattgatga ggaaagcaga caaatttata cagacaaaga aataataaat attcctgttg
    11341 aattaatcaa tggaaaatgc tcttttaaaa tagaaaaaca tttagcatca tccttaagtt
    11401 catattatga gccgggaaaa aaggatataa gaggatttag aatgattgct tcctggggag
    11461 aaaacgagtg tgagtatgcc tttataatta aaacatatga aatagacaga accgagtatt
    11521 tgacaggtga aataataact gacggtgatt atgaaatact atcaagcggc ataggctgta
    11581 tttgctttgt tcctgataag gagagtaggg aaattatcga agataaatac agttcttatg
    11641 acctttacta tttaaatgat gaaagttact ttttatatta tgacgatata tctgt
//
LOCUS       DAOPUE010000057        11481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5741_length_11481_cov_9.258008, whole genome shotgun sequence.
ACCESSION   DAOPUE010000057 DAOPUE010000000
VERSION     DAOPUE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5741_length_11481_cov_9.258008"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      9..102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            168..911
                     /locus_tag="PKH42_06340"
     CDS             168..911
                     /locus_tag="PKH42_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOA20086.1"
                     /translation="MSSNTEPQKFTSLTVVRAGLFISMSLVLKVMFEVYIPLAGIPAL
                     RINFANIPLMLSGIILGPFTGFLTGAAADIINFIAKPGGPFFPGFTLASGLSGFIPGI
                     IFKYFKRDINYNLVNTTFISLLSAGFILVFMQNGLLTFDNGILYDGKPLNPLYAAGFF
                     VLVAVYLIIPIKLSSATKDLSMNKIVFAISITQLITSIILNTYFLYILYGIGIMITLP
                     ARILTNFFMIPLYSIIIGAILNAVKGRIK"
     gene            complement(1011..1925)
                     /locus_tag="PKH42_06345"
     CDS             complement(1011..1925)
                     /locus_tag="PKH42_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20087.1"
                     /translation="MRKFILFITVFTLMLTIGCTQIPKSEETGLGPEGYEGIASTEEI
                     KKENSSLKEELEKIKTEFEVLEENYLRLVKNNESIISKLEETESKLEIISSEDIPEFK
                     SEETDKDSIVSYLNDTSKLIDDSIKGIEIISSGESVVFRTLGYGEEYSQIFIWDEGEN
                     EPVLIDGAVIEKGGSYEWLGKYILIKNKDKNKVLDIENKKITGSFDEPQKLQLLEGTS
                     AILLKDKDNNFVLYDFINDNSKQINLDNNKYTDFDLRNNIIVFSGAYVENDVEYEIRA
                     SIALDKLIEAFDTEGSEEAESEEIEDSV"
     gene            complement(1978..>3138)
                     /locus_tag="PKH42_06350"
     CDS             complement(1978..>3138)
                     /locus_tag="PKH42_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20088.1"
                     /translation="DLEEYKPTTTVSGMITEVQINKTESYIYVKNYSEVESIVKINGD
                     TKITINGRTRELSDLKKDMLVICRINEERLALEIEADSTKDVVRGTIYYVAYVEPASI
                     TIYDEDDDKLTFDIGKDVEVLHDGKATELRRLKKNDEVTILLDDDEVVKISSISRIKH
                     YDGVITSIDYNYPIKVTIKTDEDVSKTFIFNKDVEVTRNDDDSSFDQVRVGDEVTITT
                     EYDEMIAINTIAKEAETSGVIREIIIAPQSKIKIADEDGEIKQYSVSSNVKITIGAKN
                     VSIYDLRLGYNVYINTSGDEIVTMEVSEMETAKSFTGKIIYINEDDGLIMMQSVTSTG
                     KELIYLSVTNSTIIYDTSGKTRYFKDLEEGESIVSIAVQQGGEYIATSIMIQ"
     assembly_gap    3139..3238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3239..3495)
                     /locus_tag="PKH42_06355"
     CDS             complement(<3239..3495)
                     /locus_tag="PKH42_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20089.1"
                     /translation="MKKLNRIILLSVVLALLLTMTANAAVFSDISNHWARSYIERVEK
                     NGLVSGYEDGTFKPDNNVTVLESLVMMSRLYKIDDDIREEI"
     gene            complement(3609..4541)
                     /gene="arcC"
                     /locus_tag="PKH42_06360"
     CDS             complement(3609..4541)
                     /gene="arcC"
                     /locus_tag="PKH42_06360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOA20090.1"
                     /translation="MSNIIVLALGGNALGNDPQEQKEAVKHTSKSIVDLIEAGNQVIL
                     SHGNGPQVGMINLAMDAASKSEAGTPEMPFPECGAMSQGYIGYHLQNAIQNELKLRGI
                     KKSVATVVTQVVVDKNDPAFQNPTKPVGSFYTKEEAELMTAKGFTFKEDAGRGYRRVV
                     PSPKPIDIVEIDFIKSLVDTGNIVIAAGGGGIPVVEEADGLTGIDAVIDKDFTTAKLA
                     ELVDADSLIILTAVEKVAVKFGKPEQKWLSELTVEEAEKYIADKEFAEGSMLPKVRAA
                     ISFATSKTGRKALITSLEKAAEGLEGKTGTWIIA"
     gene            4711..5970
                     /gene="aroA"
                     /locus_tag="PKH42_06365"
     CDS             4711..5970
                     /gene="aroA"
                     /locus_tag="PKH42_06365"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOA20091.1"
                     /translation="MLSKNYKKKSLLIKPSLLKGKITIPPSKSISHRAIICASLSMEE
                     SKISNIVFSDDIRATIEGMKTLGADIKEADDFILVKRSNKILNNAIDCNESGSTLRFL
                     IPLSLVLKDECTFKGKGRLNERPLDIYYEIFRKSNIDYETNAGKLPLKIRGRLKGGFY
                     KIPGNVSSQFITGLFFSLPLLDVGSEIKIIGEPESKNYIKLTLDVMKRYNVNIEKIDE
                     NNYYIKGAQKYKAFDYSIEGDFSQAAFFLAANVLGCKIECLGLNYDSLQGDKEILNIA
                     EKYSSPMEEITIDASQIPDLVPVISVLASLKENCTTKIINAGRLRFKESDRLEATASE
                     MKKLGALIEETKNGLIINGKSSLYGNATVDSHNDHRIAMALAIATIKCDNPIVLEGYE
                     SVKKSYPKFWDDYQSLGGAIYELNDWK"
     gene            5948..7057
                     /gene="aroC"
                     /locus_tag="PKH42_06370"
     CDS             5948..7057
                     /gene="aroC"
                     /locus_tag="PKH42_06370"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOA20092.1"
                     /translation="MSSMIGNKLKISIFGESHGEAIGVVIDGLPSGYAINLDYINQQM
                     NRRLPGKSNLSTERKEEDEYKILSGFLEGKTTGTPLSAIIYNKDKKSEDYSDRFKLRP
                     GHSDYTGYIKYSSYNDYRGGGHFSGRLTAPLLFAGAIAMQLLESREIFIGSRIKSIYN
                     INDENIENIDAITIKNLKKMSFPIISLEKGALMKTAIIEQKEKGNSLGGIVETFIINI
                     PAGYGEPFFDSVESKISHIVFSIPAIKGIEFGAGFKIAELTGKEANDEFKVENNKIIT
                     STNNNGGILGGITNGMPIVFRTAIKPTPSISLSQNTVDISSMENTIINTKGRHDPCIV
                     PRVVPVIEGACALALLDLILERDGEKWSLIKQEQK"
     gene            7027..7287
                     /locus_tag="PKH42_06375"
     CDS             7027..7287
                     /locus_tag="PKH42_06375"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOA20093.1"
                     /translation="MELDKARTEINKIDKEIVRLLEKRFNIVNEIGNYKRKQGLSIYD
                     ETREKQVVESCISYLENKEYSKFIDDIYFQIMKCSKDIQQEG"
     gene            7291..7794
                     /locus_tag="PKH42_06380"
     CDS             7291..7794
                     /locus_tag="PKH42_06380"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOA20094.1"
                     /translation="MKNIVIIGMPGSGKTTVGMMLSKKLNMEFFDMDDNIVSMENKSI
                     NEMFEISEEYFRNAETRCAKFLNNKTSLIIAAGGGIIKRKENIDYLKQNSIIVFLNRP
                     PEKIIEDIDIKTRPLLREGRDKVFTLYNERLHLYKKYCDIEVLNDKTLDDAVNEIIKR
                     VIPYISS"
     gene            complement(7841..9394)
                     /locus_tag="PKH42_06385"
     CDS             complement(7841..9394)
                     /locus_tag="PKH42_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOA20095.1"
                     /translation="MSGDTLQIFVSMIIYMAVVIGIGLYFAKRANESSENYFLGGRTL
                     SPLVTAMSAEASDMSGWLLMGLPGVAYWYGLSDAAWTAIGLAAGTYLNWLFVAKRLHN
                     YSIAAGNSITLPDFLSNRFKEKNKIIMSISAVFILVFFIVYASSCFVTVGKLFNALFG
                     FKYQYMMILGAAFVLFYTFIGGFLAESASDTLQAFVMIIALVTIFFVGTLAAGGVAQV
                     IENAKEIPGFFEFFGIASPNVTDGVQEVVNGVPQYNEAGEYGLYTILSTLAWGLGYFG
                     MPQVLLRFMAIRDSNELKTSRRIATVWVVISLGAATAIGIIGRAVFPTTLLTYSDSES
                     IFIVLSSNLLPAFLAGFVMAGILAATISSSDSYLLIAASAFSKNIYQHLIKKDATDKQ
                     VMNVSRIILVLICIIGIFIALDENSVIFTIVSFAWAGFGATFGPIIIFSLFWKRTTRE
                     GAIAGMLAGGIMVFVWKLVLRPMGGLFGVYELLPAFIVSCLFIYAVSKMTAEPSEEIQ
                     KEFEAVMKM"
     gene            complement(10015..11049)
                     /locus_tag="PKH42_06390"
     CDS             complement(10015..11049)
                     /locus_tag="PKH42_06390"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOA20096.1"
                     /translation="MILNELKIGNLTARIPIVQGGMGVGVSLANLAAAVANEGGIGTI
                     SGVQIGFREPDFFTDPIGANLRAIKKEIKKAREKTKGIIAMNFMAAMNNYELYVKKAV
                     EEKIDLIVSGAGLPMALPRLVKGSNTKIVPIVSSEKAAKIIIRSYLKSDRLPDAVVVE
                     GPLAGGHLGFKMEELLENKCMALIDIVKEVKLLLSTFEEKYKVKIPLIAAGGIRTKED
                     IIELKEAGADGFQIASLFVPTVECDAHQNFKSAYINARDDKINIIKSPVGMPGRAIET
                     NFLTQTERIIKKCHKCMPNCNPNEIPYCISEGLINSVEGRDGLIFSGAKLNNVNKMTT
                     VKEVINNLIR"
     gene            complement(11219..>11481)
                     /locus_tag="PKH42_06395"
     CDS             complement(11219..>11481)
                     /locus_tag="PKH42_06395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOA20097.1"
                     /translation="KLAAVEKEVQLLAAEIEKTRRRVNALEYIMIPQLEETIKYIQMK
                     LDENERGNQTRLMKVKDMMVKEAIEEKQQFTKNAIDEYANNK"
BASE COUNT         3621 a         2194 c         1787 g         3779 t
ORIGIN      
        1 ataaaaaatt cagagtagat aagatgcgtt aagtgttgag ggatgggatg tcgccctcaa
       61 acgaaagact tatgtctgcg gtttcttatc gcttccgctg tattagagaa acatatgttg
      121 ctcctatatt gtgcggaggt attgctttgc tcaataagga ggaaaatatg agtagtaata
      181 ctgagccaca aaaattcaca tcattaactg ttgtaagagc agggcttttt atatcaatga
      241 gccttgtgtt aaaagtaatg tttgaagtat atatcccttt ggcaggaata ccggcactta
      301 ggataaactt cgctaacatt ccgttgatgc tttcaggcat aatattagga ccgtttacgg
      361 gatttttaac cggagccgct gcagatataa tcaactttat cgcaaagccc ggagggccct
      421 ttttccccgg gtttacctta gccagcggat taagcggttt cattcccgga attattttta
      481 aatattttaa aagggatata aattataatt tagtcaatac aacttttatt tctttattgt
      541 ctgccggttt tattttggtt tttatgcaaa atggattatt gacatttgac aatggtatac
      601 tttatgacgg gaagccttta aatcctttat atgcagcagg gttctttgtg cttgtagctg
      661 tatatcttat aattccaatt aagttgtcat ctgcaacaaa agacctgagc atgaataaaa
      721 tagtttttgc aatcagtata actcagctga ttacatcaat aattttgaac acttactttt
      781 tatatatttt atatggcata ggtataatga ttaccctgcc ggcgagaata ttgacaaact
      841 tctttatgat acctctttat tcaataatca tcggtgctat attaaatgct gtaaagggaa
      901 ggattaaata aaaatctttc acaggttatt catagcaatg acgaaagctt attatttgaa
      961 aaattaaagg gcaggtaata aatctgccct tatctttgta ccttaataca ttaaacacta
     1021 tcttctattt cttctgattc tgcttcttcg cttccttccg tatcaaatgc ttcaattaat
     1081 ttatccaaag caatggatgc ccttatttca tattccacat cattttcaac ataagcacct
     1141 gaaaatacga ttatattgtt tcgtaaatca aaatcagtat atttattatt gtctaaatta
     1201 atttgtttac tgttgtcatt tataaaatca tacagaacaa agttattatc cttgtcttta
     1261 agcaaaattg cagaagttcc ctccaataat tgcaattttt gcggctcatc gaaggagcct
     1321 gttattttct tgttttcaat atctaatacc ttgttcttat ccttattctt aattaaaata
     1381 tacttgccga gccattcata ggagcctccc ttttcaatta ctgctccgtc aatcaacacc
     1441 ggctcatttt ccccttcatc ccaaatgaat atttgactgt actcttctcc gtatcccaag
     1501 gttctaaata ctacactttc accggaactt attatttcaa ttccttttat actgtcatca
     1561 attaattttg aggtgtcatt caagtatgaa acaatgctgt ccttgtctgt ttcttctgat
     1621 ttaaattcag gaatatcctc actgctgatt atttctaatt ttgattctgt ttcttccaac
     1681 ttagatataa tactttcatt gtttttgact agtcttaaat aattctcttc taatacttca
     1741 aattctgttt taattttttc caattcttct tttaggctgc tattttcttt tttaatttct
     1801 tcagtagagg caataccttc atatccttcg ggccctaaac ctgtttcttc agatttgggt
     1861 atctgagtac agcctattgt aagcattagg gtaaaaactg ttataaacaa tataaatttt
     1921 ctcatactct cacatccttt cgcatcccta tagagaaaaa agcatctttc gatgctttta
     1981 ctgaatcatt atacttgttg ctatgtattc tccgccctgt tgaacagcta tacttacaat
     2041 actttctcct tcttccaaat ctttgaaata cctggtttta cctgaagtat catatattat
     2101 cgtgctgttg gtaactgaca ggtaaatgag ttctttccct gttgacgtga cactttgcat
     2161 cattattaat ccatcatctt cattaatata tataatctta ccagtgaagc tcttggcagt
     2221 ttccatttct gaaacttcca tcgtaacaat ttcatctccc gaggtgttta tataaacatt
     2281 atatccaagg cgcaaatcgt aaatcgatac attttttgct ccgattgtaa tcttaacatt
     2341 gctgctcacg gagtactgct taatttctcc atcttcgtct gcaattttta ttttgctttg
     2401 aggtgcaatt attatttccc tgattactcc gcttgtttca gcttctttgg caatagtgtt
     2461 aattgcaatc atttcatcat attctgtagt gattgtaact tcatcgccga ctctaacctg
     2521 gtcaaagctt gagtcatcat catttcttgt tacttcaaca tccttgttaa aaataaatgt
     2581 tttagaaaca tcttcatctg tttttattgt aacctttata ggataattat aatcaatgga
     2641 tgtaataact ccgtcataat gtttgatacg gcttatggaa ctaattttaa caacctcgtc
     2701 atcatccaat aaaatggtca cttcatcatt ctttttaagt cttcttaatt cagtcgcctt
     2761 gccgtcatgt aatacttcaa catctttccc gatatcaaat gtaagtttat catcatcttc
     2821 atcgtaaata gttattgatg caggctcaac atatgccaca taataaattg tacctcttac
     2881 aacgtccttt gtgctgtcag cctctatttc taaggcaagt ctctcctcgt taatcctgca
     2941 gataacaagc atgtcctttt ttaaatctga taactctctt gttctcccgt ttatcgtaat
     3001 tttagtatca ccatttattt taacaatact ttcaacttcg ctgtaatttt tcacgtatat
     3061 gtaggattct gtcttgttaa tttgtacctc ggtaatcata ccgctgactg ttgtggtagg
     3121 tttatattct tctaaatcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     3241 tatttcttct ctaatatcat catcgatttt atataatctc gacatcataa ccagggattc
     3301 caatacggtt acattgttgt ccggtttgaa agtaccatct tcatatccgg aaacaagtcc
     3361 atttttttca actctctcta tatagcttct tgcccaatga tttgatatat cactaaagac
     3421 agctgcattt gcagtcattg taagcaacaa agccagcaca acagaaagta aaataattct
     3481 atttaatttt ttcataatac ctcccacatg tccgattttt aataaattat atcatataga
     3541 tgttgatact gcaataagaa tcgacaattt gtaaacattc cttacacccg ttatttgtca
     3601 tcctgagctt aagcgattat ccatgtaccc gtctttcctt ccaaaccctc agccgctttc
     3661 tccaatgaag taattaatgc ctttctgccc gtcttggatg tggcaaagga tatggcagcc
     3721 cttacctttg gaagcatgga gccttctgca aattccttgt ctgcaatata cttttcagct
     3781 tcttccacag tcagttcaga taaccatttc tgttccggtt tgccaaattt cacagctacc
     3841 ttttctacag ctgttaatat tattaagctg tcagcatcaa ctaattcagc aagctttgct
     3901 gttgtaaaat ccttatcgat aactgcatca atgcccgtta atccgtcagc ttcttccact
     3961 acgggaattc cgccgccccc tgcggcaata acaatattac ctgtatcaac caatgattta
     4021 atgaaatcaa tttctactat gtctattggt tttggagaag gtactactct tctgtacccc
     4081 ctgcctgcat cctctttaaa tgtaaatcct ttagctgtca ttaactctgc ttcttcctta
     4141 gtgtaaaaag agcctactgg ttttgtagga ttttgaaatg cagggtcatt tttatcaacc
     4201 acaacttgag ttactactgt tgcaacagat ttttttatcc cccttagttt caattcgttt
     4261 tgtatagcat tttgtaaatg ataaccgata taaccttgac tcatggcacc gcattccgga
     4321 aaaggcattt ccggagtgcc tgcttccgat tttgaagctg catccatagc taaatttatc
     4381 attccgactt gcggaccgtt cccatgagat aaaattacct gatttcctgc ttcaattaaa
     4441 tcaactattg attttgaagt gtgtttaact gcttcctttt gctcctgcgg gtcattaccc
     4501 aatgcatttc cgcctaatgc caatactata atgttgctca taattcctcc ttgtatataa
     4561 caagggacaa ggaatgtccc tatttacgta aatttattgt accatcatat tttcaattta
     4621 gcaatttact tttatctgct attcaaaaaa ctattgacat accatgatat tgtgataaat
     4681 tttaatcatt accgtatagc aggtgttatt atgttaagca agaactacaa aaaaaagagt
     4741 ttgttgataa aaccttcatt attaaaaggg aaaattacta tcccaccgtc aaaaagtata
     4801 agccacagag caataatatg tgcttctctc tccatggaag aaagcaaaat cagcaatata
     4861 gttttttcgg atgatataag agcaacaatt gaaggaatga aaacattggg tgcggatatc
     4921 aaggaagcgg atgatttcat tctcgttaaa aggagcaata aaattttgaa taatgcaatt
     4981 gactgcaatg aatcaggctc aactctgagg ttccttatac ctttgtcctt ggttttaaaa
     5041 gatgaatgca cttttaaggg aaagggacgt ttgaatgaaa ggcccttgga tatatattat
     5101 gaaattttta ggaaatcaaa tattgactat gaaacgaatg caggaaagct ccctctcaaa
     5161 ataagaggca gactaaaagg cggtttttac aaaatcccgg gcaatgtaag ttctcaattt
     5221 atcaccggat tatttttttc ccttcctctt cttgatgttg gttcagaaat taaaatcatc
     5281 ggtgaacccg aatcaaagaa ttatatcaag ctgacactgg atgtcatgaa aagatacaat
     5341 gtaaatattg aaaaaataga tgagaataat tattatataa aaggggctca gaaatataaa
     5401 gcatttgact acagtattga aggtgatttt tcacaagcag cattttttct tgctgcaaat
     5461 gtattgggat gcaaaattga atgtctaggt ttaaactatg attcactgca gggtgacaaa
     5521 gaaatattaa atatagcaga aaaatattct tcgcctatgg aggaaataac gatagatgct
     5581 tctcaaatac cggacttagt tcccgtaata tcggttcttg catccttgaa agaaaactgc
     5641 acaaccaaaa ttataaatgc aggaagactg agatttaaag aatcagacag gcttgaggca
     5701 actgcctcag aaatgaaaaa gctaggagct ttaatagaag aaacaaaaaa tggacttata
     5761 attaatggaa aaagctcctt gtacggaaat gcaaccgtag acagccataa tgaccataga
     5821 attgccatgg cattggcaat tgcaacaatt aaatgcgata atccgattgt tttggaagga
     5881 tatgaatcag tcaaaaaatc atatccaaaa ttttgggatg attatcaaag cttaggaggt
     5941 gctatttatg agctcaatga ttggaaataa actaaaaata agtatattcg gggaatcaca
     6001 cggagaagca ataggtgttg taattgacgg actgccatca ggatatgcaa taaatttgga
     6061 ttacataaat caacagatga acagacggct tcccggaaaa agtaatttgt caaccgaaag
     6121 aaaagaagaa gatgaatata aaatactcag cggtttctta gaaggaaaga ctacaggaac
     6181 tccgctgtcc gcaataattt ataataaaga taagaagtct gaggattaca gcgataggtt
     6241 taagcttcgc cccggtcaca gcgattacac gggttacata aaatattcat catataatga
     6301 ctaccgggga ggaggtcatt tttccggaag gcttaccgca ccgcttttat ttgcaggagc
     6361 aattgctatg caattattgg aaagcagaga aatatttata ggcagccgca taaaaagcat
     6421 ttacaatata aatgacgaaa atatcgaaaa tattgatgca attacaataa aaaatctaaa
     6481 gaaaatgagt ttccccatca tttcattaga aaaaggagcc cttatgaaaa cagccataat
     6541 tgagcaaaaa gaaaaaggaa actccttggg aggaatagtg gaaacattta tcataaatat
     6601 tcctgcaggg tacggggagc cgtttttcga ttcggttgaa agcaaaatat cacatatagt
     6661 attctccata cctgcaatta aaggaattga atttggtgca ggttttaaaa ttgctgagct
     6721 tacgggaaaa gaagccaatg atgaatttaa agtagaaaat aataaaataa ttacttctac
     6781 caataataac ggaggtatat tgggaggaat aacaaacgga atgcccatag tatttagaac
     6841 cgcaataaaa cccactcctt caatatcctt gagtcagaat acggttgata tatcatctat
     6901 ggaaaatact atcataaata ctaaaggccg tcacgatccc tgtattgtac ctcgagttgt
     6961 tcccgttata gaaggagctt gcgctcttgc cttacttgat ttaattttgg aaagggatgg
     7021 tgaaaaatgg agcttgataa agcaagaaca gaaataaaca agatagataa agaaatagtc
     7081 agactattgg aaaaaagatt taatatagtg aatgaaatag gtaattacaa aagaaaacaa
     7141 ggactttcca tatatgatga aacaagagaa aagcaggttg ttgaatcttg cataagttat
     7201 ttggaaaata aggaatattc caagttcatt gatgacattt actttcaaat aatgaaatgc
     7261 agcaaggaca tacaacaaga aggataaaaa atgaagaata tagttataat cggcatgccg
     7321 gggagcggaa aaacaactgt tggcatgatg ctttcaaaaa aattaaacat ggaatttttc
     7381 gatatggatg ataatatagt atccatggaa aacaaaagca ttaatgaaat gtttgaaata
     7441 agcgaagaat atttcagaaa tgcagaaacc agatgtgcaa aatttttgaa caataaaaca
     7501 tcgcttataa tagctgccgg cggaggaata ataaaaagga aagaaaatat agactatttg
     7561 aaacaaaatt caattatagt attcttaaac agacctccgg aaaaaatcat agaagatatc
     7621 gacataaaaa cccgccctct cctaagagaa gggcgagata aagtatttac cctatacaat
     7681 gaaagacttc atctgtataa gaaatattgc gacattgaag ttttaaatga taaaacactg
     7741 gatgatgccg taaatgaaat tataaaaagg gttatccctt atatatcatc ctaatggtgc
     7801 caaaggtgcc tggcaccaaa attatttggc accaaaatta ttacattttc attaccgctt
     7861 caaattcttt ctgtatttct tccgaaggct ccgcagtcat tttagatact gcataaataa
     7921 acagacagga tactataaat gcgggaagaa gttcatatac tccaaagagc ccgcccatag
     7981 gccttaaaac aagcttccaa acaaatacca ttataccccc tgccagcatt cctgcaatag
     8041 caccttccct ggttgttctc ttccagaaca atgagaatat gatgatcggt ccgaaggtag
     8101 ccccgaaccc tgcccaagca aaggatacta ttgtgaaaat aacactgttt tcatcaaggg
     8161 ctataaatat acctataata caaatcaaaa ccaagataat ccttgaaaca ttcataacct
     8221 gtttgtcggt tgcatccttt ttaattaaat gctggtatat atttttggaa aatgccgaag
     8281 ctgcaatcaa taaatatgaa tcagatgaac ttattgttgc tgctaaaatc cctgccatta
     8341 caaaaccggc tagaaatgca ggaagtaaat ttgatgaaag tacaatgaaa atactttctg
     8401 aatcactgta tgtcaaaagt gtagtaggaa atactgctct tcctattatt cctatggctg
     8461 tggcagcacc taaggaaata actacccata ccgttgcaat tcttcttgat gtttttaact
     8521 cattagaatc tctgatagcc atgaatctca ataatacctg tggcattccg aagtatccca
     8581 atccccatgc aagagttgat aaaatcgtgt aaaggccata ctctcctgct tcgttatatt
     8641 gaggtactcc attaacaacc tcttgcacac catccgttac attaggtgag gctataccga
     8701 aaaattcaaa aaacccgggg atttcttttg cattttctat aacttgtgct actcctcccg
     8761 ctgcaagagt tcccacaaaa aagattgtga ctaaagcaat aatcataaca aatgcctgca
     8821 atgtatcgga ggcgctttct gccagaaaac ctcctataaa tgtgtaaaat aatacaaagg
     8881 cagcccctaa tatcatcata tactgatatt taaatccaaa cagtgcatta aaaagtttac
     8941 ccaccgttac aaaacagctg gaagcataaa caatgaagaa tactaaaata aataccgcag
     9001 aaatagacat aattatttta ttcttttctt tgaaacgatt gcttaaaaaa tcaggaagtg
     9061 ttattgaatt acctgctgca atcgaatagt tgtgaagcct ttttgcaaca aacaaccagt
     9121 ttaaatacgt tcctgccgct aatcctattg cagtccatgc agcatcactt aagccatacc
     9181 agtatgcaac tccgggaagt cccattaaaa gccagccgct catatctgat gcttcggcac
     9241 tcatagctgt aaccaatggg ctaagtgttc tacctcctaa aaaataattt tcactgctct
     9301 cattggcacg ttttgcaaaa tataatccga tgcctataac aacagccatg taaataatca
     9361 ttgatacaaa aatctgtagt gtatctccac tcataatgaa acctccttct ttaatatttt
     9421 gttattatga ttttctttct ccctacagta gaatttgtgt ccccgtaccc tagaaataca
     9481 gggttattat taaaaaatcc gcctcaaaaa gaggcggatg tccgcggtac cactctaact
     9541 gcattatata cagcatctca tttaaataag gggtgttccc tttgggttgt tactcccaaa
     9601 cgctctgagg cgggttcaat caacattggg acaaaggtat ttcaccggcc aaccttctct
     9661 ctaagtccat gtttcattac tattcctctt cacagcaaat attaatatta aaaaacatac
     9721 tacaccaata caaatacttt gtcaagatat tttttgaatt attttaccta aaaattcata
     9781 taaatttaat tcaattgttt ttacatattg tcttgtcccc acaataaaaa caaatatgta
     9841 ttttaattca taatatgctt ccatcccctg ctatgttaca aaattctctt tcccttgtca
     9901 tacatgaaaa cacccccgtc cccttgtcat aatgcgacaa ggagacaagt tgttatcatt
     9961 atagcaactg tccccttgtt gcattagagg aattaaaata ttatcttaat cttattacct
    10021 gattaaatta ttaataactt cttttactgt agtcatttta tttacattgt ttaattttgc
    10081 acctgaaaaa atgagaccgt ctcttccttc tacggaattt atcaaccctt ccgatatgca
    10141 atatggaatt tcattaggat tgcagttagg catacatttg tggcattttt ttataattct
    10201 ttccgtttga gtgagaaaat ttgtttctat agctcttccc ggcattccga ccggactttt
    10261 gataatatta atcttatcat ctcttgcatt gatatatgct gacttaaaat tttggtgagc
    10321 atcacattca accgtgggaa caaataagct tgctatttga aaaccatctg ctcctgcttc
    10381 tttaagctca atgatatctt cctttgtcct tattccaccg gctgcaatta aagggatttt
    10441 tactttatat ttctcttcaa atgtacttaa tagaagtttc acctctttaa caatatctat
    10501 taatgccatg catttatttt ccaataattc ttccatctta aaacccagat gtccacctgc
    10561 caagggccct tcaactacta ccgcatccgg cagtctatct gatttgagat aacttctgat
    10621 tattattttt gcagcttttt cagaagatac tattggaact atcttcgtat tgctcccctt
    10681 cactaatcta ggaagtgcca tcggaagtcc tgcgcctgat acaattaaat ctattttttc
    10741 ttcaacagcc tttttaacat acaattcata attgttcatt gctgccataa aattcatggc
    10801 aataataccc tttgtctttt ctcttgcctt ttttatttct tttttgattg cccttaaatt
    10861 tgcccctatg gggtcagtaa aaaaatcagg ctctctgaag cctatctgaa caccggatat
    10921 agtgcctata ccaccttcat ttgcgactgc tgccgctaaa tttgccaaag atacaccaac
    10981 gcccatgcct ccctgaacaa tgggaattct agcagttaag tttcctattt tcaattcatt
    11041 taatatcata tttccaccat tattttagaa atcaattaat ttgatattca aagtatatat
    11101 gcatatgttt cttttgtcaa gtaaaaaaaa cagggacatt ccttcaattt taaaggcgtg
    11161 tcccctgctt tgagattctt cgtacagtgc actcagaatg acaataatta attaactttt
    11221 atttattatt tgcatattcg tcaatagcat tcttggtaaa ctgttgtttt tcttcgattg
    11281 cttctttaac catcatgtct ttaactttca tgagcctggt ttgatttcct ctttcatttt
    11341 cgtcaagctt catttgaatg tacttgatag tttcttcaag ctgaggaatc attatatatt
    11401 caagtgcatt aactcttctt ctggtttttt cgatttcagc tgcaagaagt tgaacttctt
    11461 tttcaacagc tgccaatttt a
//
LOCUS       DAOPUE010000058        11450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5770_length_11450_cov_7.350856, whole genome shotgun sequence.
ACCESSION   DAOPUE010000058 DAOPUE010000000
VERSION     DAOPUE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5770_length_11450_cov_7.350856"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PKH42_06400"
     CDS             complement(<1..1268)
                     /locus_tag="PKH42_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOA20098.1"
                     /translation="MKTQKFDVTGMTCSACSARIEKNINKTEGILEANVNLLTNSMTV
                     KYDESVLSEEDIIKVVEDTGYGASAAMQKNKEIKKDAKDDKSEIREMKKRVIISLIFA
                     IPLFYISMGHMLNWPLPKIFHGTENALIFAFTQFLLCLPVIIVNNKYYKVGFKTLFKG
                     SPNMDSLIAIGTSAAAAYGVYAIYKIGYGLGHMDMDMVRQFSMDLYFESAAVVLALIT
                     LGKFLEARAKGRTSDAIKKLIDLSPKTALVERLGIEMEIPVEDVVKGDIIIVKPGQSI
                     PVDGVIVFGSSSVDESMLTGESIPVEKKVGDKVVGASINKSGFFKFEAQKVGDDTTLA
                     QIIKLVEEASSSKAPISKMADKISGIFVPIVIVIAVLATIVWLILGADFEFALSIGIA
                     VLVISCPCALGLATPTAIMVGTGKGAENGIL"
     gene            complement(1282..1545)
                     /locus_tag="PKH42_06405"
     CDS             complement(1282..1545)
                     /locus_tag="PKH42_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOA20099.1"
                     /translation="MKADKDKINRLLKTAAGQIEGISKMVDEDRYCVDISNQILAVQS
                     ILKKANNEILKAHLHMCVKEAFNEGNEDEKIDEIISIINKVSK"
     gene            1729..3261
                     /locus_tag="PKH42_06410"
     CDS             1729..3261
                     /locus_tag="PKH42_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOA20100.1"
                     /translation="MRKEKIIIGTLILTIATTSVRFVGMFFRIYLANTIGSEGMGLYQ
                     LILSFFFLMVTLATSGIRVAISKLISEQIALGNYLNAKKVLHQSIGISIFTGFAAGAV
                     LYYYADYIGSNILNDDRTVLALLYLAPSLPIMAISNCYKGYFYALGKVTQPAIIQMTE
                     QFVRIFLIIYLMGTFLPKGLEYGCAAVAIGMTAEEIFALFLVWFFYIFDKKPRLTKTQ
                     TKADNMVFKILQISVPVSATSYINSILRTVENTLIPARLLLYGLSSEIAISLYGMIKG
                     MVLPLLFFPSSILTSLSSLLIPAVAGDNALANEKSVSRTLSQVIHFTTISGVLVVAVF
                     VSFPNEIAMAVYNNRQVGLMIKLISYVCPFMYLNMVISSMLNALGEQVSSFIVNIIES
                     VLKIFIIYVFVPVYGFNAYLFALFMTTILNTILYLFRLLQVSSIVFDISDWIFKPIVA
                     AVIAGILSRYSYFLFVQNSRHIISLISSICILCLLYLMGLIAFKSVRLESINDLKKYV
                     LK"
     gene            3388..3927
                     /locus_tag="PKH42_06415"
     CDS             3388..3927
                     /locus_tag="PKH42_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOA20101.1"
                     /translation="MHYNTMDSLLFFLIYGFLGFLLETVFRSIADRKVYISRGFLTNG
                     FCPLYGICALLIIEGFILCELALSSPLSAMIVATAGSIFLVTLIEYIVGRMLDRVFHY
                     KLWDYSAYRLNLHSYICLEFSLLWGIMALLLVYFMHPVMEVLVFAIPYNTRAVAVVLF
                     LSMLIINASYQIRKRVYGG"
     gene            complement(3956..5575)
                     /locus_tag="PKH42_06420"
     CDS             complement(3956..5575)
                     /locus_tag="PKH42_06420"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOA20102.1"
                     /translation="MKYNLIFGHKTPDTDSVCSAIALSHLKNKLNEPSKAYILGDINK
                     ETAFVLKYFNVSVPEILDNVKIQIKDLDFERVKPFTKNKSVHFAYFHMNENKLRTLPI
                     VDEENNLCGLITMKDIAMSLINTDQHYLCTSYDNIIETLLGKSILKFDEIIKGYVIVA
                     AFEEKTLRKSDIVNENSIVITGDRYDVIKYAIESKVRLIIVTGDLKVTDELIEMAIKN
                     KVNIIGTAYHTYYTAKNISLAKYVDDIMKKNDIMMFSEEDYLNDCKEDIEQSKHSKFP
                     IISKDKKYLGLLSRSHLINPKKKKVILVDHNEMNQSADGIEEAEILEVIDHHKIGDIK
                     TSIPISFRNTPVGSTSTIIFQMYRENNVELSKDIAGLILSGIISDTLLFKSPTTTGRD
                     KYAVNELLKIVDLDLHEYAMDMFRAGTSLEGKTLEEIIYQDFKKFNLVYKNVGISQVF
                     TLDINEIMNRKEEFVKLIDDITSDKYYLIIMAVTDIVNEGSYIFYTSSKEKLVRMIFE
                     EDVHQGIYIDKCISRKKQIVPKVINALNQLK"
     gene            complement(5665..6651)
                     /locus_tag="PKH42_06425"
     CDS             complement(5665..6651)
                     /locus_tag="PKH42_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA20103.1"
                     /translation="MAMNKQIIENIMTDYNKKRLRAAHEADKKKEKLYAALPRLSEID
                     KQMKLLSISLSKSFLSHPEDLDVQVKKLRKEIEELKKERQSIYEENNIPKDYLEIEYE
                     CKKCSDTGYTTDGKKCSCFKRQIINNLFQMSNMVNMLQKENFNTFDINVFSNESYKNE
                     KLTPRQNMYYVLEISQDFCSNFHETNMNLLFFGGTGLGKTFMCNCIANELLNKEISVL
                     YQTSFSLFETIENHKFNKLNETEENLMNYNMIFDCDLLIIDDLGTEVSNSFTNAELFN
                     IINERMITDKRTIISTNLSLEQLRDTYSDRITSRIFNNFVPLKFYGKDLRFK"
     gene            complement(6638..7699)
                     /locus_tag="PKH42_06430"
     CDS             complement(6638..7699)
                     /locus_tag="PKH42_06430"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOA20104.1"
                     /translation="MYFIFQEMQIDLGDTPIENIFINDYMPQADGTYVKVYLMGYKYA
                     KDRLDFNNETISKNLNLPLSDVLNAWNYWENEGIIIKHETDDEYNYIVEFVNLKQFYV
                     DNVYKHIRNVNDKNSYVGNDKLISTARNPEIKTMMEEIEDMLGRPLKVSEKQKIITWL
                     NKYKTKPYMMTQAFSYSVNNKKIKRFNYIESVVSAWYDAGVVDLDTMVEYLEKRNDRF
                     SAYSRISKALGFNRTLTEAEMRTIDRWIDEWDFSMEMILKCLDNSTKINNPNLNYFDK
                     ILSEWYKNGFKTIEDLKNDKKPEKTKKETSKCKNKFHNFIQSEDISGDDLEKIAREKF
                     KKKLNKLGLAIPEENNGNE"
     gene            7945..9423
                     /locus_tag="PKH42_06435"
     CDS             7945..9423
                     /locus_tag="PKH42_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOA20105.1"
                     /translation="MKIKDLTFNIFNKNLNSKIFLQLDEKKDRQPLKLTKRTKGTIAL
                     LLVTSMILSVVYASDYLKTHENNVFATDSFTIVSGENELCRVRDPELLNSVLKKIDKD
                     LENRLHHEIKIESKYSLKPSKAKDKEIATEDELYDLINSNLIYSIMAYEINVNGERIG
                     IVNSEYEAKSIIEEVKHHFSKDYDSESLLEVTTVEDIEIKQVKATNAEIQEKEKLVDY
                     IIKGTDEEKKYTVEKGDSYWSIAEYFEMELEELLVANSASEDDIIQIGDELNLIVPKP
                     FINVQVKRKVVQEEKMPYDTEYVKVSYMYNDEEIVTKAGKYGVSEIEAIVTEQNGIQI
                     AKEVLSEKVITEPVTRIVTTGTQDPPPKQGTGYFINPLPGSMITSRFGNRAGGFHRGQ
                     DMAKAAGSAIKAADGGTVLFAGWSGSYGYMVDIDHGGGFTTRYAHCSDIYVSAGEKVY
                     QGKIIAAVGSTGVSSGPHLHFEVRKYGSLVNPASYIGVQYRE"
     gene            9601..10089
                     /locus_tag="PKH42_06440"
     CDS             9601..10089
                     /locus_tag="PKH42_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA20106.1"
                     /translation="MLYLDNRFMEVALPIIEHEEYQQMRYIKHHDESVFEHSLKVAFY
                     SYQFAYKQNLDWESTIRGALLHDFFLYKFKKCFSLRIITDSIKHAIIHPKIAFDNAVK
                     FFDLNKKEENIIKGHMFPFGFPKSKEAWIVSFVDKYIAVFEYSSNFKKMMHKKQPAYQ
                     AE"
     gene            10301..10831
                     /gene="raiA"
                     /locus_tag="PKH42_06445"
     CDS             10301..10831
                     /gene="raiA"
                     /locus_tag="PKH42_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOA20107.1"
                     /translation="MKISIYSKNLQLTDALREASIKKLRRLDKFFQQDIEAKVVLSIE
                     KKIHKVEVTIPFNGRIVRVEEYSDDMYNALDEAVESLEQQIRKHKTKLQNKRYSSQSI
                     KFENIEPLDYEEEEEFKVVKTKRFAIKPMNIEEAILQMDLLKHNFFVFLNADTEEVNV
                     VYKRKDGNYGLIEPEL"
BASE COUNT         3727 a         1930 c         1805 g         3988 t
ORIGIN      
        1 agaattccgt tttcagcacc ctttccggta ccgaccatta tagcagttgg tgttgcaaga
       61 cctaaggcac aaggacaaga aataaccaag actgctattc ctattgaaag agcaaattca
      121 aaatctgctc ccaatatcag ccatactatt gttgccaaaa cggctatcac aattactatc
      181 ggaacaaata taccgcttat tttatcagcc atctttgata tgggagcctt tgaagagctc
      241 gcttcctcaa ccagcttaat tatttgagct aatgtagtat catctcctac cttctgtgct
      301 tcaaatttaa agaaacctga tttattaata cttgcaccta ctaccttatc tccgaccttc
      361 ttttcaacag gtatactttc tcccgtaagc atggattcat ctactgatga agagccgaat
      421 actattacgc cgtcaacagg tattgattgt ccgggcttaa caattataat atcccccttt
      481 accacatctt caaccggtat ttccatttca attcctaatc tttcaaccaa tgcagtcttt
      541 ggggataaat caatgagttt ttttatagca tcggaagtcc tccccttcgc ccttgcttcc
      601 aagaatttac ccaatgttat taaggctaaa acaacggctg cagattcaaa atataaatcc
      661 attgaaaact gcctaaccat gtccatatcc atatggccaa ggccatagcc gattttatat
      721 atggcataaa ctccgtaagc cgcagctgct gatgtaccta tggcaatcaa tgaatccatg
      781 ttgggagaac ccttaaacaa agttttaaaa ccaaccttgt aatatttatt gttaacaata
      841 attaccggaa ggcagagcaa aaattgtgta aatgcaaaaa tcaaagcatt ttcagttccg
      901 tgaaatattt tgggcaaagg ccagttaagc atgtgcccca tggaaatgta aaataagggt
      961 atagcaaata tcaatgatat aatcactctt tttttcattt ctctgatttc ggatttatca
     1021 tcttttgcat cctttttaat ttctttattt ttttgcatgg cagctgaagc accgtatccc
     1081 gtatcttcaa caaccttaat tatatcctct tcgcttaata cggattcatc atactttacc
     1141 gtcatgctgt tagtgagcaa atttacattt gcttctaaaa tgccctcggt tttattaata
     1201 tttttttcta ttcttgcaga acatgcagag caagtcatgc cggttacgtc aaatttttgt
     1261 gttttcatta gtgtcactcc tttatttaga aaccttgttg attattgaaa taatttcatc
     1321 aattttttca tcttcatttc cttcattaaa agcttctttt acgcacatgt gcaaatgcgc
     1381 cttcaaaatt tcattgtttg cttttttaag tatagactga accgccaata tttgatttga
     1441 aatatccacg caatacctgt cctcatcaac cattttggaa atgccttcaa tttgtccggc
     1501 tgcagttttt aacaatctat taattttatc tttatcagct ttcattattt cacctctttg
     1561 gccttaattc cctacccccc tggggggtat gtttttataa taaaccacct tttttcatat
     1621 gtcaagagaa aaatataata attacatttt taattatacc ctcaatggct taaaataaat
     1681 cgaaattttc agcatatagt atattagttg aaaattttga ggtgaggtat gagaaaggaa
     1741 aaaattatta ttggtacatt aatattaaca atagctacta cttctgtccg ctttgtggga
     1801 atgttcttca gaatatattt agccaatacc ataggctcgg aaggaatggg tttgtatcaa
     1861 ttaattcttt cctttttctt ccttatggtc acccttgcaa cttcgggaat cagggtagct
     1921 atttcaaaac tgatttcaga acagattgct ttgggaaatt atttgaatgc taaaaaagtt
     1981 ctccaccaat ccataggtat ttctattttt acaggttttg cggccggtgc tgtactgtat
     2041 tactatgctg attatatagg ctctaatata ttgaatgatg acaggacggt attggctttg
     2101 ctgtacttgg ctccaagcct ccccataatg gcaatttcaa actgctacaa aggttatttc
     2161 tatgccttgg gtaaagtcac tcaaccggca attatacaga tgaccgagca atttgtaaga
     2221 atatttttga taatctattt gatgggtact tttctcccca aaggtttgga atacggatgt
     2281 gccgcagttg caatcggaat gactgcagaa gaaatatttg ctttattttt ggtttggttt
     2341 ttctatatat tcgataaaaa acctcgcttg accaagacac agacaaaggc ggataatatg
     2401 gtttttaaaa tcctgcaaat ttctgttcct gtttctgcca cttcttatat taactcaata
     2461 ttaagaacag ttgaaaatac attaattccg gcaagacttt tactgtacgg tttatcttcc
     2521 gaaattgcca taagccttta tggtatgatt aagggtatgg tgcttccctt attgttcttt
     2581 ccttcttcaa tattgacatc tctttcatcc ttgttgattc ctgcagtagc gggagataat
     2641 gcgcttgcca atgaaaaaag tgtttcgaga acactgtctc aggtaatcca ctttacaact
     2701 atttctggag tattggtggt agcggtattt gtatcattcc ccaatgaaat agccatggct
     2761 gtttacaaca accgccaagt gggtctcatg ataaaattaa tatcatatgt gtgtcctttc
     2821 atgtatttaa acatggttat atcatccatg ctaaatgctc ttggagagca ggtaagctct
     2881 tttattgtaa atattattga atctgtgttg aaaattttta tcatatacgt atttgtccct
     2941 gtttacggat tcaatgcata cttgtttgca ttgtttatga ctacaatact taatacgatt
     3001 ttatacctgt ttagattatt gcaggtttca agcattgttt ttgatatttc cgactggatt
     3061 tttaaaccca ttgttgctgc agtaattgcc ggaatattat ctagatattc atattttttg
     3121 ttcgtgcaaa attcccgtca tattatttcc ttaatatcgt caatatgcat attatgcctc
     3181 ttatacttaa tgggactgat tgcttttaaa agtgtacgac ttgaatccat aaatgattta
     3241 aaaaaatatg ttttaaaatg atgacaagac ccccgtcccc cttgtcataa aactggaaaa
     3301 ttcaataatt caaaacataa attggcattc ttataatagc atatttctta aattactcca
     3361 aagaatacat ttcaaggagt ggagaaaatg cattataata ctatggattc attgttgttt
     3421 tttttaatat acggattctt gggattcttg cttgaaacag tctttagaag tattgcggac
     3481 agaaaggttt atataagcag aggatttctt actaatggat tttgtccctt atatggaatc
     3541 tgcgcattat tgataattga gggctttata ttgtgcgaat tagcgctaag cagcccctta
     3601 tcggcaatga tagttgcgac agcgggaagt atttttttgg tcacgctgat tgaatatata
     3661 gttggtagaa tgctcgacag ggtatttcat tataaattat gggattacag tgcatacagg
     3721 ttaaacctgc attcatatat atgccttgaa ttttcattgc tttggggaat tatggcactt
     3781 ttgttggtat attttatgca tcccgtgatg gaggtactgg tgtttgcaat accatataat
     3841 actagggcag ttgctgtagt tttattttta tcaatgttaa ttattaatgc atcctaccaa
     3901 ataagaaaga gggtctatgg aggatgagac cctttaagga attaatccgg tgaaattatt
     3961 ttaactggtt tagggcgttt attactttgg gaactatttg tttttttctt gagatgcatt
     4021 tatctatata gataccttgg tgtacatctt cttcaaaaat cattcttacc aacttttcct
     4081 ttgatgaagt ataaaaaata taagagccct cgtttacaat atccgttact gccattatta
     4141 ttaagtagta tttatcagag gtaatatcgt cgataagctt tacaaactct tccttcctgt
     4201 tcattatttc attaatatcc aaggtgaata cctgggatat acccacattc ttataaacca
     4261 agttaaattt tttaaaatcc tgataaatta tttcttccaa tgtttttcct tccaaggatg
     4321 ttcctgccct aaacatatcc attgcatatt catgtaaatc taaatcaaca atttttagca
     4381 gctcgtttac tgcatatttg tctcttccgg ttgtggtagg agacttaaaa agcagcgtat
     4441 cggagattat tcccgacagt atgagcccgg ctatatcctt tgataattct acattgttct
     4501 ccctgtacat ttgaaaaatt atggtgcttg tgcttccaac aggtgtattt ctgaaactga
     4561 tcggaataga agtcttaata tccccgattt tatggtggtc tataacttcc aatatttcgg
     4621 cttcttcaat cccatcggca ctttgattca tttcattatg gtcaaccaaa attaccttct
     4681 ttttcttcgg attgattaaa tggcttctgc taagaagccc cagatatttt ttatcctttg
     4741 atattatggg gaattttgaa tgcttggact gctcaatatc ctccttgcag tcattaagat
     4801 aatcttcctc tgaaaacatc ataatatcat tcttcttcat aatatcatcc acatatttag
     4861 caagagatat attctttgcg gtataatatg tatgataagc agtgcctatt atattaacct
     4921 tatttttaat tgccatttca ataagctcat cagtaacctt caaatccccc gtgactatta
     4981 tgagtcttac ttttgattca atggcatact ttatcacatc atatctgtcg cctgtgatta
     5041 caattgaatt ttcatttaca atgtctgatt tgcgaagtgt tttttcctca aaggcagcaa
     5101 caattacata acctttgatt atttcatcaa atttcaaaat actttttcca aggagtgttt
     5161 caattatatt atcataagat gtgcatagat aatgctggtc tgtatttatc aaactcatgg
     5221 caatatcctt cattgttatt agcccgcaca aattattttc ttcatcaact ataggaagag
     5281 tcctaagttt gttttcattc atgtgaaaat aagcgaagtg tactgattta ttcttggtaa
     5341 agggcttaac tctttcgaaa tctaaatcct ttatttgtat ttttacatta tccagaattt
     5401 ccgggacact tacattgaaa tattttaaaa caaaggctgt ttccttgtta atatcaccta
     5461 atatatatgc ttttgaaggt tcattgagct tattttttaa gtgactaaga gcgatagcag
     5521 aacatacgct gtcagtgtcc ggggttttat gcccgaaaat taaattatat ttcataaaat
     5581 acctcattta cgtaatatta gtgagtttca gagtgaaagg aatcgtcccc caaatatcga
     5641 ggactaaaag aaccgtaccc gtaactattt gaagcgaagg tcttttccgt agaatttaag
     5701 aggaacaaaa ttattgaata tacgggatgt aattctgtca gaataagtat ccctaagctg
     5761 ctccaaggat aaattagttg aaattatcgt tcttttatcg gtaatcattc tttcatttat
     5821 aatattaaat aactccgcat tggtaaatga attgctcacc tctgttccaa ggtcatcaat
     5881 aataagcaaa tcacaatcaa aaatcatatt gtaattcatc aggttttctt cagtttcatt
     5941 cagcttgttg aacttatgat tttcaattgt ttcaaacaag gaaaaagaag tttggtacaa
     6001 aacagaaatt tctttattca aaagttcatt tgcaatacag ttgcacataa atgtcttgcc
     6061 taagccggtt cctccgaaaa ataaaagatt catgtttgtt tcatgaaagt tgctgcaaaa
     6121 atcctgactt atttccaaaa cataatacat gttctgcctt ggtgtaagct tttcgttttt
     6181 atagctttca ttgctgaaaa cattgatgtc aaatgtatta aagttctctt tttgaagcat
     6241 attaaccata tttgacattt gaaacagatt atttattatt tgtcgtttaa aacagctgca
     6301 ctttttcccg tcagtagtat aaccggtatc actgcacttt ttgcattcat attcaatttc
     6361 caaataatcc tttggaatat tgttctcttc gtaaatagac tgcctttctt ttttaagctc
     6421 ttctatttcc ttccttaatt ttttaacctg tacatccaaa tcttcaggat gggataaaaa
     6481 actcttggat aaactgatac ttaataattt catttgttta tcaatttcag acaggcgcgg
     6541 aagagcagca tatagttttt cctttttttt atctgcttca tgggcagccc ttaatctttt
     6601 tttattatag tctgtcataa tattttcaat tatttgttta ttcattgcca ttgttttcct
     6661 ccggaattgc taatcctaat ttattaagct tctttttaaa tttttctctt gcgattttct
     6721 ctaaatcgtc tccagaaata tcctcgcttt gaataaaatt atgaaacttg tttttacact
     6781 tggaagtttc cttttttgtt ttttcaggtt ttttgtcatt ttttaaatct tctatagttt
     6841 taaaaccgtt tttgtaccat tcactcagaa ttttgtcgaa ataatttaaa ttaggattat
     6901 taatttttgt tgaattgtct aaacatttta aaatcatttc catagagaaa tcccattcat
     6961 caatccatct atcgattgtt ctcatttcag cctcggtaag tgttctgtta aaacccagtg
     7021 ctttgcttat ccttgaataa gctgagaacc tgtcattacg tttttcaaga tactcaacca
     7081 ttgtgtccaa atctaccact ccggcatcat accatgctga aacaacagat tctatgtaat
     7141 tgaatctctt aatcttttta ttgttaacag aataactgaa agcttgagtc atcatatatg
     7201 gtttagtttt atatttgttg agccaagtga ttattttttg tttttcactg acttttaaag
     7261 gtctccctaa catatcttct atttcttcca tcatggtttt tatttcagga tttctggcag
     7321 tagaaattaa cttatcatta cccacataac tatttttatc gttaacattt cgaatatgtt
     7381 tgtacacatt atcgacatag aattgtttaa gatttacgaa ttcaactata tagttatatt
     7441 catcatctgt ttcatgttta atgattatac cttcattttc ccaataattc catgcattca
     7501 aaacatcgct caaaggcagg tttaaattct ttgaaatagt ttcattgtta aaatccagcc
     7561 tgtccttagc gtatttgtat cccatcagat atacttttac ataagttccg tcagcttgag
     7621 gcatgtagtc attaataaaa atattctcta taggggtatc tcccaggtct atttgcattt
     7681 cttgaaaaat aaaatacatg aatttctcct ttttctacat aatttgccat aaagattgta
     7741 atataattgt aacaatcttg ttataataat gtgaaaacat tttctcctaa aaatattgac
     7801 ataatgacat gtaatagaat ataatgtctt aaagatatat taaagacatt ttaattaact
     7861 ttattatatc atttttttat ataaaagaaa aatgttattt ttcttgtgat agtagtagaa
     7921 aattatagct aggaagtaga acttatgaaa atcaaagatc taacatttaa tatttttaat
     7981 aaaaatttaa actccaaaat attcctgcaa ttagatgaaa aaaaagatag acaacccctc
     8041 aaacttacca aacgaaccaa aggaacaatc gcattattac tcgtaacaag catgatatta
     8101 agcgtagtct acgcttcaga ttatttaaaa acccacgaaa acaatgtatt tgcaacagac
     8161 agctttacta tagtatccgg agaaaatgag ctgtgccggg taagagaccc ggaactgctg
     8221 aattcagtat taaagaaaat agataaagat ttagaaaata gattacatca tgaaataaaa
     8281 atagaaagta aatacagctt aaaaccctca aaagcaaaag acaaagaaat tgcaacggaa
     8341 gatgagcttt acgatttaat taattcaaat ttaatttatt ccattatggc atacgaaata
     8401 aatgttaatg gagaaagaat agggattgta aattccgaat atgaagcaaa aagtataatt
     8461 gaagaagtta agcatcattt ttccaaagac tatgacagcg agtcactgct ggaagttact
     8521 acggttgaag acatagaaat aaagcaggta aaagcaacta atgcagaaat tcaagaaaaa
     8581 gaaaaactgg tagattacat aattaaagga accgacgaag agaaaaaata tacagtagaa
     8641 aaaggcgatt catactggtc catagccgaa tatttcgaaa tggaattaga agagttgcta
     8701 gtagcaaact cggcatctga agatgatatt attcaaatag gagacgaatt gaatttaatt
     8761 gtaccaaaac cttttataaa tgtacaagta aagcgaaaag ttgtacagga agaaaaaatg
     8821 ccttatgata cagaatacgt aaaagtttca tacatgtaca atgatgaaga aattgttaca
     8881 aaagccggta agtacggtgt ttcggaaatc gaagcaattg tgaccgagca aaacggtatt
     8941 caaatagcaa aagaagtatt gtcggaaaaa gtgataacag agcctgtaac tagaatagta
     9001 acaacaggaa cacaagatcc gccgccgaaa caaggaacag gatattttat aaatcctctt
     9061 ccgggctcaa tgataacttc gcgattcggc aacagagccg gaggcttcca tagaggccag
     9121 gatatggcaa aggcggcagg ttcagcaata aaggctgctg acggaggaac agtattattt
     9181 gcaggctggt cgggaagcta cggatacatg gttgacatag accatggagg cggttttacc
     9241 acaagatatg cacactgcag cgatatttac gtatctgcgg gagaaaaggt gtaccaaggc
     9301 aaaataatag cagccgtagg ctctacagga gtatcatcag gacctcatct gcactttgaa
     9361 gtaagaaaat acggctctct ggtgaatcct gcatcatata taggggtaca atacagagaa
     9421 taacatttat aaactcataa atacagacaa gccgatgaaa aatcatcggc ttttatattt
     9481 agtcttacct tcatctaatt tttgcatgtt ttattaaaaa tttttattgc aattttttga
     9541 gtttcattat aagatgagaa agtattatgc aatgcgcaac tactaataaa ggacggacag
     9601 atgttatatt tagataatag gtttatggaa gtagcacttc caattattga gcatgaagaa
     9661 tatcagcaaa tgagatatat taagcaccat gatgaaagtg tatttgaaca ttcattaaaa
     9721 gtagcatttt attcatatca atttgcatat aaacaaaatt tagactggga atcaacaata
     9781 agaggggcct tgctccatga cttttttcta tataagttta aaaaatgttt tagtttgaga
     9841 ataataacag attcaataaa acatgcaata attcatccaa aaattgcttt tgacaatgca
     9901 gttaaatttt ttgatttaaa caaaaaagaa gaaaatatca taaagggaca tatgtttcct
     9961 tttggttttc cgaaatcaaa agaagcatgg attgtaagct ttgtggataa gtatattgct
    10021 gtatttgaat actcttctaa ttttaagaaa atgatgcata agaaacaacc ggcataccaa
    10081 gccgaatagt attatggcta taaataattc catagtgtca ttctgagggc attgcccgaa
    10141 gaatctcata agagccttcg cttacgctca ggatgacagt atataattag acaccccaac
    10201 ggatgtcttt tttttatttt gacaaatatt ttgtttattt ttctcataaa tgggtaaaat
    10261 ttaaatatat ataaataaat acttgaaagg ggctgactaa atgaaaatat ctatctacag
    10321 caaaaatttg caacttaccg atgcattaag agaggcatca attaaaaagt taaggaggtt
    10381 agataaattt tttcaacaag acattgaggc aaaagttgtt ttaagtattg aaaagaaaat
    10441 acataaagta gaggtcacaa ttcccttcaa tggaagaatt gtacgtgttg aagaatattc
    10501 ggacgacatg tacaatgcat tagatgaagc agttgaatct ttagagcagc aaataaggaa
    10561 acataaaaca aaactgcaga ataaaaggta ctcaagtcaa tcaataaagt ttgaaaacat
    10621 cgagccctta gactacgaag aagaggaaga atttaaggta gtaaaaacca agcgctttgc
    10681 aattaagccc atgaacatag aggaagcaat actgcaaatg gatttattaa aacacaattt
    10741 ctttgttttc cttaatgcag ataccgaaga agtaaacgta gtatataaaa ggaaagatgg
    10801 aaattacggt ttaattgagc cggaattgta aaacctaact ttaaatatag atatcaaaaa
    10861 gacgcagcac gcgtcttttt ttacttttac ctgcatcctt aacgtaccat aatacttaaa
    10921 cacttaatag ttatataaga aatacgtttg catgattgtc gaataatgta taaatttagc
    10981 acttatgctt tgcttataat tataccttgt taataattta ggtatataaa ttaagttgtc
    11041 cacaggcgtg gataaattgt ttaatttcta ataatgctta atattgaaat ttcaacaaaa
    11101 aatatttttg tatttttgta aaaaaacact tgacaaatat ttcccttaaa gtccccgaat
    11161 ttaagaattg ttaacaattt aatccacagc tgtggataag ttgaaaacat atagaacata
    11221 tcttctattt ttttgagatt gttaaatata tttaaccggt tttaatctta tttaattagt
    11281 tgaacattcc atggaaaagt ctggaatgtt aattttctat ttttttgaat aattaataat
    11341 taagtttact tgtaatttta taaaactaat aattattata cacatttttc acaactgcct
    11401 taattacaat tcattaagaa caaaaattta tcttaatgaa tataatgcct
//
LOCUS       DAOPUE010000059        11283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5846_length_11283_cov_8.332918, whole genome shotgun sequence.
ACCESSION   DAOPUE010000059 DAOPUE010000000
VERSION     DAOPUE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5846_length_11283_cov_8.332918"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PKH42_06450"
     CDS             complement(<1..523)
                     /locus_tag="PKH42_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015592551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20108.1"
                     /translation="MLSVKNLNVFYGGIHALKGVTIDVQEGQIVSIIGSNGAGKSTLI
                     NAISGIVKYKDGEIIYKDEKLPKQAHKIVKAGICQVPEGRVIFANLTVMENLNMGAFL
                     RNDKDGIQKDLERIFKLFPILKERQKQMAGTLSGGEQQMLAMGRGLMSNPELILLDEP
                     SLGLAPLLINTIFD"
     gene            complement(516..1289)
                     /locus_tag="PKH42_06455"
     CDS             complement(516..1289)
                     /locus_tag="PKH42_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20109.1"
                     /translation="MSVLSLNNLTKKFGGLTAVDNVTFNIEEGEIFGLIGPNGAGKTT
                     IFNLITGIYSITSGEIFYYDKKIENLKPFQIANMGITRTFQNIRLFKKLTAYDNVLTA
                     CHKLADYNLFDSVIRFRKFKPQEKFINEKTAELLKLMGLWDYKDIVASNLPYGLQRKL
                     EIARALALEPKLLLLDEPAAGMNPEETIQLMGLIKEIRDEFKLTVLVIEHHMDLVMGV
                     CDRIFVLNFGIPLALGTPKEIQKDKRVIEAYLGKEEGHA"
     gene            complement(1286..2188)
                     /locus_tag="PKH42_06460"
     CDS             complement(1286..2188)
                     /locus_tag="PKH42_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA20110.1"
                     /translation="MSWGYLSGILILTGINLMTVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYTAAMFTLKFNILPESMFQLQFYLALIAGGLVAMIFSLIIGKLTLNLKGDYFCIATL
                     GFGEAIRLILDNVDYFGGARGLSSITTQGTTTLTNVVIINIIAITVLVFIIKSRHGRN
                     MVAIREEELAAQTIGIDVFKYKMISFAISAFYAGIAGGLMAHYFGYIQPIMFKLVKST
                     ELTIIVIFGGLGSVSGSVVGTILLTFLPELLRSFANWRLVLYGAAVIIIMISRPQGLM
                     GGKEFSIKGLVRFIAKRRKEGVKS"
     gene            complement(2199..3080)
                     /locus_tag="PKH42_06465"
     CDS             complement(2199..3080)
                     /locus_tag="PKH42_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOA20111.1"
                     /translation="MSYFLQQLINGLSLGSVYALMSVGYSLVYSIMNFSNFAHGGVIM
                     IGAYIGYFCLTLLGVPFFTAFILSAVGSGILAVSMERIVYRPLRKRNAPFLYFIISAM
                     GASIFLENIVIASPIGPTFRSYPAVFSTSPIMIGGIALGRIDLLMFIISTLSLIGLMY
                     IIEKTKIGLSIRATSYSIKASTLMGVNVDKVIFIIFLLGGLLAGLAGMLFGMKYIVYP
                     QIGNITTKSFIAAVFGGLGSLPGAIIGSILLGLIETMTSGYLSSEYRDLIAFVLLIGV
                     LIFKPTGLMGKTTEDKA"
     gene            complement(3121..4284)
                     /locus_tag="PKH42_06470"
     CDS             complement(3121..4284)
                     /locus_tag="PKH42_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20112.1"
                     /translation="MSKKFLTLISVVLIFTMIVTACSSGTGTPKEETIKIGWIGSLTG
                     DQAVWGTCESNTVKMLIEEVNASGGILGKQVEGIYYDTKGDAAEAVNATKRLASQDKV
                     VAIIGPNASGQAIAISSVLEEYKVPDISTVGTNPKVTVNDDGSVKEFNFRVCFIDPYQ
                     GAVAAGYAYDRLGLRNAAVLYDVASDYSQGFTEFFEKTFIEKGGKIVAKEGFKEGDVE
                     FKPQLSKIKDANPDMILMPYYYKEVALSANQARELGIDAVLIGGDGWPSEQLMTMAAD
                     AIEGSYIVNHLDFNDPEVKPLQEKYTAKYNLPMELNGYMAHDAFLLLKAAIEDAGVAD
                     PVKIAESLTKVTVQGVTGTIKLSPEDHNPIGKEAAIVRVENADYIFQEKYGLQ"
     gene            4573..8889
                     /locus_tag="PKH42_06475"
     CDS             4573..8889
                     /locus_tag="PKH42_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOA20113.1"
                     /translation="MEYFVGLDIGSTTVKIAVLDFFGKIVYKQYERHYSDITNTVTEI
                     INNSSEHLKDSFIRINVTGSGAIDVSKYLDIPFVQEVAASTTAIEKYIPSTDVVIELG
                     GEDAKITYLSGNLEQRMNGSCAGGTGAFIDQMAVLMKTDAAGLNELSRKHKTIYPIAS
                     RCGVFAKSDIQPLLNDGARQEDIAVSIFQAVVNQTISGLAQGRPIKGNVAFLGGPLYF
                     LSELRERFKETLNLTENNFVCPDDANYYVAIGAALMSIENKKISFNDFYDSIKTKIEN
                     SNFTYRKIDLNPLFKNKDEYNEFIERHNKHKAKRKNIKEYFGDAFLGIDAGSTTTKLV
                     LITKDGEILYSYYGSNEGNPLNSALDAVKGLYSQLHSGIKIAYTAVTGYGERLIKAAL
                     NVDIGEIETVAHYKAADFFLKGVDFIIDIGGQDMKSMVIKNGVIDSIMLNEACSSGCG
                     SFIETFAKSLDMTVEEFAQEATWSKNPVDLGTRCTVFMNSKVKQAQREGATISDISAG
                     ISFSVIKNALFKVIRLNNLDSLGEKIVVQGGTFYNDSVLRAFEQLTGREVVRPDIAGI
                     MGAFGAAVIAKNKYSEEKESIFVKAADLDNFSFNTSMERCGLCSNNCLLTVNKFSDGR
                     YYVSGNRCERGAEKYISITKKESLPNLYKYKTKRVFNYKPLPLSEATRGIIGIPRVLN
                     MYEDFPFWFTFFSELKYRVVLSGISSKKIYELGMGTIPSESVCYPGKIAHGHITDLIN
                     KKVKKIFYPCISYNIKEDESAGNHYNCPIVVSYPESIEANIDMKDVVFYKPFLNLNDK
                     ERLIEKLYRELGEAEGLTLFEIRSAAEKAYEELFSYKQDVRNEGERALTYISENNIKG
                     IVLAGRPYHIDPEINHGIPEMIESYGLAVLSEDSISDKGTVERPLRVIDQWSYHTRLY
                     KAATFVSNTDMLELIQLNSFGCGLDAVTTDQVREILEKNNKIYTVIKIDEITNLGAVR
                     IRLRSLLASMEEREKNGVVHKYSPSNFKRVIFTKEMKENHTILIPQMSPIHFQFISTA
                     AKMSGYRAVVLPAVDKKAVDAGLKVVNNDACYPSIIVIGQLINALTSGKYDLNNTSLM
                     ISQTGGGCRATNYIGFIRKALKDSGYGDIPVISFNVVGLEKQPGFKLTAPFFNRLIMA
                     IVIGDTLMRTTYRTRPYEKIKGSINSLYEKWVKKAHMIIEKANIKEFKNLIQNIVTDF
                     DNVELDESIKKPKVGIVGEILVKFHPNANNDVFSLLEEEGAEVVVPDLLDFFSYTAYN
                     GVIKYDELLIGSKATKAINNIAVKIIEFYKRPSIDAFKNSKRFLTPVGIKELANKAKN
                     LLSLANQCGEGWFLTAEMIELLESGINNILCLQPFACLPNHITGKGMIKKLKEAYPWS
                     NIVPIDYDSGMSEVNQINRIKLMLTNAVRNMKQELDTALLNSIEENAPDSNLQNMKEI
                     EI"
     gene            complement(8915..9910)
                     /gene="argF"
                     /locus_tag="PKH42_06480"
     CDS             complement(8915..9910)
                     /gene="argF"
                     /locus_tag="PKH42_06480"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOA20114.1"
                     /translation="MAVNLKGRSFLTLMDFTPEEIRYLLDLSADLKRKKRSGSTDYLL
                     KGKNIVLLFEKTSTRTRCAFEVAALDEGAHVTFLDSGSSQMGKKESLEDTAKVLGRFF
                     DGIEYRGYEQKVVEDLAKFAGVPVWNGLTDVDHPTQILADMLTIEEHVAKPLNKVKVV
                     FAGDIRNNMSYAWMYGCAKMGMHFVAFGPKVLADQLDKEILKKVNEVAEYTGAKIEIS
                     DSIDSVKGADVIYTDVWASMGEEALIPDRVKLLTPYKVTMDLLKATENEDVIFMHCLP
                     AFHDFETKLAKEQKELGYDIREVTDEVFRSRHSVVFDEAENRLHTIKAVIVATIG"
     gene            complement(9993..11210)
                     /gene="arcA"
                     /locus_tag="PKH42_06485"
     CDS             complement(9993..11210)
                     /gene="arcA"
                     /locus_tag="PKH42_06485"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOA20115.1"
                     /translation="MNKSINVYSEIGKLKKVLLHRPGRELENLMPEYLDRLLFDDIPY
                     LKIAQQEHDAFANIFRENGAEVVYLEDLVVESLINDEVNSKFIDEYIEEAGIKEDRET
                     GILKEYFLSFSDKKDMVLKMMEGIRKSEIKDYRGPGLADFLSNQYPFIIDPMPNLYFT
                     RDPFAALGSGVSIHKMMTATRNRETLFGKYIFNYHPTYKDTKLFYDRTEDYSLEGGDI
                     LVLNKETIAVGISQRTNPNAIEKFANIILTEESSFKKVLAIDIPKTRAFMHLDTVFTM
                     VDYDKFTIHPNIKSDIVVYVLKKIDGKMDITEERATLEQVLEKELNIDKIKLIKCGGD
                     SVIDASREQWNDGSNTLCISPGEVIVYSRNYVTNKILEEEGIKIHIMPSSELSRGRGG
                     PRCMSMPLIRENV"
BASE COUNT         3624 a         2198 c         1846 g         3615 t
ORIGIN      
        1 tatcaaatat agtatttatc aataaaggtg caagccctaa tgaaggttca tcaagaagga
       61 tgagctcagg atttgacata agacctcttc ccattgcaag catctgctgc tctcctcccg
      121 aaagagttcc tgccatttgt ttttgtcttt cctttaatat ggggaatagt ttaaatattc
      181 tttccaaatc cttttgaata ccgtctttat catttcttaa aaaagcaccc atatttaaat
      241 tttccattac ggtgagattt gcaaaaataa cccttccttc aggaacttga caaattcctg
      301 cttttactat tttatgagcc tgtttaggaa gcttttcatc cttgtaaata atttcaccat
      361 ccttatattt tacaatcccg ctgattgcat tgataagggt agactttcct gctccgttag
      421 agcctataat cgaaactatc tgaccttcct gaacatctat ggtgactccc ttgagagcat
      481 gaattccgcc ataaaaaaca tttagatttt taacactaag catgaccctc ctccttccct
      541 agataagctt caataactct cttatctttt tgaatttcct tcggagtccc gagagccaaa
      601 ggtatgccga aattaaggac aaatatccta tcgcaaacac ccattaccag atccatatga
      661 tgctcaatta cgagcacagt aagcttaaat tcgtctctta tttccttaat cagtcccatc
      721 agctgtattg tttcttcagg attcatacct gctgcgggct catcaagcaa taaaagctta
      781 ggctccaatg ccaaggctct tgcaatctca agctttcttt gcagaccata tggaagattt
      841 gatgcaacaa tatctttgta gtcccataaa cccattaact tcaacagctc agctgtcttt
      901 tcatttatga atttttcctg aggtttaaat tttctaaacc ttattacaga atcgaaaaga
      961 ttataatccg ccagtttatg acatgcagta agcacattat cataagcagt cagcttttta
     1021 aaaagtctga tgttttgaaa tgtccttgta attcccatat ttgctatttg aaaaggttta
     1081 agattttcaa tctttttgtc gtaatagaaa atttctccgc ttgtaatgct gtaaattcct
     1141 gttattaaat taaagatggt agttttgccg gcaccgttgg ggcctattaa accaaaaatc
     1201 tctccttctt caatattaaa ggtcacattg tctacagcag ttaaccctcc aaatttctta
     1261 gttaaattat taagagaaag tacactcatg atttaacccc ctctttacgt ctttttgcaa
     1321 taaatcttac tagtccttta atactgaatt cttttcctcc cataagtccc tgtggccttg
     1381 aaatcatgat aataattacg gcagctccat acagaacaag cctccagttt gcaaagcttc
     1441 gtaaaagttc cggtaaaaat gtaagcaaaa tggttcctac tacacttccg gatacacttc
     1501 ccaaacctcc aaaaattact attatggtca attcggttga cttaaccagc ttgaacataa
     1561 taggctgaat gtatccgaag taatgtgcca tcaatcctcc tgcaatacct gcataaaatg
     1621 cacttatggc aaaggatatc attttatatt taaaaacatc tattcctata gtctgggcag
     1681 ctaattcttc ttctcttatg gcaaccatat ttcttccatg gcgggacttt ataataaata
     1741 ccaataccgt tatggcaatt atattaatta tcacaacatt ggtcaatgtt gttgtccctt
     1801 gtgtagttat actcgaaagt cctcttgcac ctccaaaata atcaacattg tcaagaataa
     1861 gtcttatagc ttctccaaaa cccaaggttg caatgcaaaa ataatcaccc tttaaattta
     1921 aggttaactt tccaataatc aaactaaaaa tcatggcaac cagtccgcct gctatcaatg
     1981 ccaaatagaa ttgcaattgg aacatggatt ccggtaaaat gttaaatttc aatgtaaaca
     2041 tagcagctgt atatgcaccg attgccatga atccggcatg tccaaaagaa aatagacccg
     2101 taaatcccgt aagcaatgat aaacctaata cggtcattaa atttattcct gttaatatca
     2161 aaattcctga taaataaccc cagctcatac tctacctcct aagccttatc ttccgttgtt
     2221 tttcccatta atcccgtagg cttaaaaatt agtacgccta taagaagtac aaatgcaatt
     2281 aaatctctat attctgaaga tagataaccg gaagtcattg tttctataag tccaaggagt
     2341 atggagccga ttatggcacc cggcaaactg cctaatcctc cgaaaacagc agctatgaat
     2401 gatttcgttg taatattgcc aatttgggga tatactatat atttcattcc aaaaagcatg
     2461 cccgccagac ctgccaataa accgccaagc aaaaatatta tgaatataac cttatccaca
     2521 ttaactccca tcaaagtact ggcttttata ctgtaagatg ttgccctgat tgacaatcca
     2581 atcttggttt tttctattat atacataaga cctattaagc ttagggtaga tattatgaac
     2641 attaataagt caattcgacc caatgcaatt cctccaatca ttatgggaga agtagaaaaa
     2701 acagcgggat aagatctgaa tgtaggtcct ataggtgatg caattactat gttctccaaa
     2761 aaaattgaag cacccattgc agatattata aaataaagaa atggtgcatt tctttttcta
     2821 agtggtctgt aaacaatcct ttccatgctt acagccaata ttcctgagcc gactgcggat
     2881 aatataaagg cagtaaaaaa aggcaccccc agcaacgtaa gacagaaata tccaatatat
     2941 gctccaatca taataacccc gccatgagca aagttagaaa aattcataat actatataca
     3001 agagagtatc ctacagacat taaggcatat acgctaccta aggacaaacc gttaattaat
     3061 tgctgtaaaa aatagctcat attacctcct caatgatagg cgggtttaac ccgcctattt
     3121 ttattgtaat ccatactttt cttggaatat ataatctgca ttttcaactc ttactattgc
     3181 agcttctttt ccaatcggat tgtgatcttc aggactcaat ttaattgttc ctgttacacc
     3241 ctgcacagtt acctttgtca aagattcagc aattttaacc ggatcagcaa ctccggcatc
     3301 ctctattgct gcttttaaca gaaggaatgc atcgtgagcc atataaccgt ttaattccat
     3361 aggtagatta tattttgctg tatatttttc ttgaagcggt tttacctctg gatcattgaa
     3421 atccaagtga ttcactatat agcttccttc aatggcatca gcagccatgg tcattaattg
     3481 ctctgaaggc catccgtctc cacctattaa gactgcatca attcccaatt ctcttgcttg
     3541 atttgcactc aatgcaactt ctttataata gtaaggcatc aatatcatat caggatttgc
     3601 atcctttatt ttactcaact gaggtttgaa ttcaacatcg ccttctttaa aaccttcttt
     3661 tgctacaatt ttcccgcctt tttctataaa ggttttttca aagaattctg taaatccttg
     3721 tgaataatca cttgccacgt catataaaac tgctgcattc ctaagtccca atctatcata
     3781 tgcatagcct gcagctaccg caccctggta tgggtctatg aaacatactc taaagttgaa
     3841 ttcttttact gagccgtcat cattaacggt tacttttggg tttgttccaa ccgtagaaat
     3901 gtcaggaacc ttatactctt caagaacaga ggaaatagct attgcctgac cactggcatt
     3961 aggaccgata atcgctacaa ctttgtcctg tgatgcaagt ctctttgttg catttacagc
     4021 ctcagctgca tcgcccttgg tatcataata aataccttca acttgtttac ctagtatacc
     4081 gccgcttgca ttaacttctt caatcaacat ttttaccgtg ttgctttcac atgttcccca
     4141 aactgcttgg tctcctgtta aagatccaat ccagcctatt ttgattgttt cttcttttgg
     4201 tgtacctgtg ccgctactgc atgcagtaac aatcattgtg aaaatcaaaa ccacacttat
     4261 aagtgttaaa aactttttac tcataaaacc ctcctgattc ataattatta gtttacttac
     4321 aaaactgagg tcatgtaaac gttattattg tccatataat tataagcttt ttttatgtta
     4381 attacaagtt acattattgt tatattatcc caaaaatcat ccaaatgttc caataagcca
     4441 caaatttctt tatacatcgg taccccacat tggtgccagg cataaattaa gttgattttg
     4501 agcattaaaa tgatataatt atttatattt attgacagta caatgcaaat ttactcaact
     4561 ccggaggcca ttatggaata tttcgtggga cttgatatcg gctcaactac ggtaaaaata
     4621 gctgtattag attttttcgg caaaattgta tataaacaat atgaaagaca ctactcagac
     4681 attacaaata cagtgacgga aataataaac aattcatctg aacatcttaa ggacagtttt
     4741 atcagaataa atgttacagg ttcaggtgct attgatgtgt ctaaatattt ggatatcccc
     4801 ttcgtacaag aagttgctgc ctctacgaca gctatagaaa agtatatacc ctccacagat
     4861 gtagtaatag agttaggcgg agaagatgca aaaataacat atctttcagg aaatttagag
     4921 cagcgcatga atggctcctg cgcaggaggt acaggtgcct tcattgacca aatggcagta
     4981 ttgatgaaga ccgatgctgc aggtttaaat gaattgtcga gaaaacataa aactatttat
     5041 cctattgctt cacgctgcgg tgtatttgcc aaatcagata tccagccctt gttaaatgac
     5101 ggagcaagac aagaagatat tgcggtatcc atttttcaag cagttgtaaa tcaaaccata
     5161 agcggacttg cccaaggaag acctattaaa ggaaatgtag cgtttttagg aggacctttg
     5221 tatttcttgt ccgagcttag agaaagattt aaagaaacac tgaatcttac ggaaaataat
     5281 tttgtatgtc ccgatgatgc caactactat gttgcaattg gcgcagcatt aatgtccatt
     5341 gaaaataaaa aaatatcttt caatgatttc tatgatagta ttaaaacaaa aatagaaaat
     5401 tcaaatttta catatcgcaa aatagattta aatcccttgt tcaaaaataa agatgaatat
     5461 aatgaattca ttgagcgtca caataaacat aaagctaaaa gaaaaaatat aaaagaatac
     5521 tttggagatg catttttagg aattgatgca ggttcgacaa ccactaaatt agtactgatt
     5581 acaaaagacg gggaaattct ttattcttat tacggaagca atgaaggaaa tcctctgaat
     5641 tctgcccttg atgcagttaa aggattatat tctcaattac attcaggcat taaaattgca
     5701 tatacggcag tgaccggtta tggagaacgc ttaataaagg ctgcattaaa tgttgatata
     5761 ggagaaattg aaaccgttgc tcactataaa gctgcagatt tctttttaaa aggcgtggac
     5821 tttattattg acataggcgg ccaagatatg aaaagtatgg ttattaaaaa tggagttatt
     5881 gattccatta tgcttaatga ggcatgctcc tcaggctgcg gctcctttat tgaaacattt
     5941 gcgaaatctc tggatatgac tgttgaagaa tttgcgcaag aagcaacatg gtccaaaaat
     6001 cctgtagacc ttggcacaag atgcactgta tttatgaatt caaaggtaaa gcaagctcaa
     6061 agagaaggag caaccataag cgacatttct gccggaattt cattttcggt gattaagaat
     6121 gccttattta aagtaataag acttaataac ctggattcat tgggagaaaa gattgttgtt
     6181 cagggaggaa cattttacaa cgattctgtt cttcgcgcat ttgagcaatt aacgggacga
     6241 gaagttgtaa gacctgacat tgcaggtatt atgggagctt tcggtgctgc cgtgattgcc
     6301 aaaaataaat atagcgaaga aaaagaaagt atttttgtta aagcagccga ccttgataat
     6361 ttttctttca atacatccat ggagcgctgc ggtctttgct caaacaattg cttattaact
     6421 gtcaataaat tttccgacgg cagatattat gtatccggca atcgctgtga aagaggtgct
     6481 gaaaagtata tttcaattac taaaaaggaa tctttgccaa acctgtacaa atataaaacc
     6541 aagcgtgttt ttaattataa accgctgccc ttgagtgaag caacaagagg aattatcggc
     6601 attccaaggg ttcttaatat gtatgaagat tttcccttct ggtttacatt tttcagcgaa
     6661 cttaaataca gagttgtgct ttcaggaata tcaagcaaga aaatttatga gcttggaatg
     6721 ggaacaatac catccgaatc agtttgttat cccggtaaaa ttgcacatgg acacataacc
     6781 gaccttatta ataaaaaggt aaaaaaaata ttctaccctt gtatttctta taatattaag
     6841 gaagatgaaa gtgcaggcaa tcactataac tgtcctattg tagtatctta tccggaatca
     6901 atcgaagcta acattgatat gaaggatgta gtattttaca agccattctt gaatctgaac
     6961 gataaggaga gactcattga aaaactttac agagaattag gagaagctga aggtttaact
     7021 ctgtttgaaa tccgttcagc tgcagagaaa gcttatgaag aattgttctc ttacaagcag
     7081 gatgtaagaa atgaggggga aagagcacta acatatataa gcgagaataa tataaaaggt
     7141 attgttcttg caggaagacc ctaccatata gatccggaaa tcaaccacgg catacctgaa
     7201 atgatagaaa gttacggttt agctgtattg tcagaggatt ccatcagtga taaaggcact
     7261 gttgaaagac cccttcgtgt tattgaccaa tggtcttatc atacaagact ttacaaagcg
     7321 gcaacatttg tttcaaatac tgatatgttg gaattaatcc agcttaattc tttcggatgc
     7381 ggcttggatg ccgtaacaac cgaccaggta agggagatac ttgaaaaaaa caataagata
     7441 tacacggtaa ttaaaataga tgaaattact aaccttgggg cagtcagaat tcgtttgcgc
     7501 tcattgctgg cttctatgga ggaaagagaa aaaaacggag ttgttcataa gtattctccc
     7561 tcaaacttca aaagggttat ctttacaaaa gaaatgaagg agaatcatac tatactgata
     7621 cctcaaatgt ctcccattca ttttcaattt attagtactg ctgcaaaaat gtccggttat
     7681 agggctgttg ttcttccagc agtagacaaa aaagccgtgg atgcaggact gaaagtcgta
     7741 aacaatgatg cctgctatcc ttcaattata gtaataggac aacttattaa tgctttgaca
     7801 tcgggaaaat acgatttaaa caatacctcg cttatgattt ctcagacagg aggaggctgc
     7861 cgtgctacaa attatatagg atttataaga aaagcactga aggactcagg ttacggagat
     7921 attccggtaa tatcatttaa tgtggtaggt cttgaaaagc aaccgggatt taagctgact
     7981 gctccctttt ttaacagact tataatggct atagtaatag gggatactct gatgcgtacc
     8041 acttatcgca caaggcctta tgaaaaaata aagggctcca taaacagcct gtatgaaaaa
     8101 tgggttaaaa aagctcacat gattatagaa aaagcaaata ttaaagaatt taaaaatctg
     8161 atacaaaata tagttacgga ttttgacaat gtagaattag atgaaagcat aaaaaagcca
     8221 aaagtcggaa ttgtaggcga aatactcgta aaatttcatc cgaatgcaaa caatgatgtg
     8281 ttttcactat tggaagaaga aggtgctgaa gttgtagtac cggatcttct ggattttttt
     8341 tcctacaccg cttacaacgg agttataaaa tatgatgaac tgctgattgg cagcaaggcg
     8401 acaaaggcaa taaacaatat tgcagtaaaa attatagagt tttataaaag accgagtatt
     8461 gatgcattca aaaattctaa aaggttttta actcctgtcg gtattaagga attggcaaat
     8521 aaggctaaaa atttattgtc cttagccaat caatgcggag aagggtggtt tttaacggca
     8581 gaaatgattg agttattaga gagcggtatt aacaacatct tgtgtcttca gccatttgca
     8641 tgtctgccga atcacattac gggcaaggga atgattaaaa agctaaagga agcttatccc
     8701 tggtctaaca tagtacccat agattacgat tcaggaatga gcgaagtaaa tcaaataaac
     8761 agaattaaac tgatgcttac caatgctgta agaaatatga aacaggagct cgatacagct
     8821 cttttaaact ctattgaaga aaatgcacct gattctaatt tacaaaatat gaaagaaatc
     8881 gaaatataaa gcacctctaa aaggtgcttt attattatcc gattgttgct acaataaccg
     8941 ctttaattgt atgtaatctg ttttctgctt catcgaaaac aacagaatgt ctgctcctga
     9001 aaacctcatc cgtaacttcc cttatatcat aacctaattc cttttgctct tttgccaatt
     9061 tagtttcaaa gtcatggaat gcaggaaggc aatgcatgaa tattacatct tcattttctg
     9121 ttgcttttaa aaggtccatg gttactttgt atggtgtgag taattttact ctatcgggaa
     9181 tcagagcctc ttctcccatg gaggcccaaa cgtcggtata tattacgtcg gctcctttaa
     9241 cactgtcgat actgtctgat atctcaattt ttgctccggt atattcagca acttcattaa
     9301 ccttttttaa tatttcttta tctaattggt cagcaagtac cttcggtccg aatgctacaa
     9361 aatgcattcc catctttgca cagccgtaca tccatgcata tgacatattg tttcttatat
     9421 ctcctgcaaa tactactttt accttattta aaggttttgc cacatgttct tctattgtaa
     9481 gcatgtctgc caatatctgt gtcggatgat ccacgtcggt taagccgttc caaacgggaa
     9541 cgcctgcaaa tttagccaaa tcttctacaa ccttttgctc gtatcctctg tattcaatac
     9601 cgtcgaagaa tctaccgagt acctttgcag tatcttccaa agactctttt ttacccatct
     9661 gtgagcttcc tgaatccagg aaagttacat gtgcgccttc atctaatgca gcaacttcaa
     9721 aagcacatct ggttcttgta gatgtttttt caaacaataa aacaatgttt ttacctttca
     9781 ataaataatc ggtagagcct gacctctttt ttcgttttaa atccgctgat aagtcaagta
     9841 agtatctgat ttcttccggt gtaaaatcca ttaatgtcaa aaaacttctt ccctttaaat
     9901 ttactgccat ttacatcctc cattattaat tttatatcct ttgtatgctt aatatatcaa
     9961 aacattttaa atatttcaat tcttttacaa ctttatacgt tttccctgat taagggcatg
    10021 ctcatacatc ttggtccgcc cctgcccctt gataactcag agcttggcat tatatgaatc
    10081 tttattcctt cttcctcaag tattttattc gtaacgtaat ttcttgaata aacaattact
    10141 tcacccggtg atatacataa tgtattggag ccgtcgttcc attgctccct ggaggcatca
    10201 ataacactgt cgccgccgca cttaattaat ttaatcttat caatatttag ttctttttcc
    10261 aacacctgtt caagggtggc tctttcctct gtaatatcca tttttccatc aattttttta
    10321 agaacataaa caactatatc agacttaata tttggatgta ttgtgaattt gtcataatcg
    10381 accatggtaa ataccgtgtc caaatgcata aaagctctgg ttttaggtat gtctattgcc
    10441 agtacctttt taaaggaact ctcttctgtc aatattatgt ttgcaaactt ttcaattgca
    10501 tttggattag ttctttgtga aatacccact gctatagttt ctttattcag cactaatata
    10561 tctccgcctt caagagaata atcctcagtt ctatcataaa ataattttgt atccttatat
    10621 gtaggatggt agttaaatat atacttcccg aataaagttt ccctgtttct tgttgcagtc
    10681 atcattttgt gaatagatac accgctgccc agtgcagcaa acggatctct tgtaaaatat
    10741 aaatttggca tagggtcaat tataaatggg tattgattgc ttaaaaagtc agctaaaccg
    10801 ggacccctgt aatcttttat ttcagacttt cgtatccctt ccatcatttt taggaccata
    10861 tccttcttat cggaaaaaga cagaaaatat tctttcaaga taccggtttc tctatcttcc
    10921 tttatacctg cttcctcaat atactcatca ataaactttg aattaacttc atcattaata
    10981 agagactcaa cgaccaaatc ttccaaataa acaacttctg ctccattttc tctaaaaatg
    11041 tttgcaaaag catcatgttc ctgctgtgca atttttaaat acggaatatc gtcaaaaagc
    11101 aatctgtcaa gatattccgg cattaaattt tctaattctc ttccaggtct gtgcaataaa
    11161 acttttttta atttcccgat ttcggaatat acatttatcg acttattcac attatcacca
    11221 cctacatata tactaataat ctttccattg tttgtcaagc ttatttaaca tatttataaa
    11281 gta
//
LOCUS       DAOPUE010000060        11234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_5875_length_11234_cov_11.167188, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000060 DAOPUE010000000
VERSION     DAOPUE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5875_length_11234_cov_11.167188"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKH42_06490"
     CDS             complement(<1..1520)
                     /locus_tag="PKH42_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA20116.1"
                     /translation="MEYPRRSYWHDTAEGIKKPAVENADLKNKKFDILIIGAGITGLT
                     TAYLLKDCGFNIGIIEATKSGYGVTGYTTAKITIQHDLFYDYLINSFSMEEAKQYLKA
                     NEEGIRLIKRIINENNIKCDYKEQTAYVYAASVNEVEDLKKELKAYEKLGIDGFYTDT
                     VPIPNKAYGAIGVRNQGQFNPLKYLYSLYNILNQSNCQIYENVRALNIVPHDEHVDVE
                     TESGTIHADKVIVASHYPFDNSFGLYFLRLYQEKEYVIAARTNEPPFEGMYINNNDPT
                     YSLRYQFSDKENLLILAGGNHRVGEKDDENESYNELERFLNSNFKNPQIVSKWSTQDC
                     MTYDKIPYIGRYTDSIDNLYVATGFKKWGMSHSGAAALILKNKILCIEDDYSGIFNPS
                     RFTPVQSSKEFVSSVKSIALGFLNRLEEDLEELSKVNPGEGKIVDYDGKKVGVYRNEA
                     GEYFCINPVCSHLKCALAFNEAEKTWDCPCHGSRFDIKGNILEGPAVLPIDKIKIK"
     misc_binding    1640..1853
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1951..>2654
                     /locus_tag="PKH42_06495"
     CDS             1951..>2654
                     /locus_tag="PKH42_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOA20117.1"
                     /translation="MKLFKDISKADYKNNEHEVSKYWEEINLLEQSIETRKNKPSFVF
                     YEGPPTANGRPGIHHVISRTLKDSVCRYKTMQGYQVKRKAGWDTHGLPVEIEVEKQLN
                     LSTKQEIEEYGIDEFNKKCRESVFTYEALWREMTERMAYLIDLDNPYITLENDYIESE
                     WWILDKVFKEGLIYEGHKILPYCPRCGTGLASHEVAQGYEEIKTITAYAKFKKKDEDA
                     YFLAWTTTPWTLLSNVT"
     assembly_gap    2655..2754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2755..4917
                     /locus_tag="PKH42_06500"
     CDS             <2755..4917
                     /locus_tag="PKH42_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOA20118.1"
                     /translation="GIVHTAPAFGEDDYNTGLKYNLPVLNPVDDTGRFRGTPWSEMFV
                     MDADQPILRWLKENGKLYKKEPVLHNYPHCWRCHTPLLYYARPSWYIQMTKLKDLLVA
                     NNNTVNWYPDYVGEKRFGNWLENVNDWAISRSRYWGTPLPIWRCECGHIESIGSRKEL
                     VEKAVEDIDESIELHRPYVDEVHIVCPECGKHMTRVKDVIDCWFDSGAMPFAQWHYPF
                     ENKERFDELFPADFINEGIDQTRGWFYSLIAVSTLVTGKSPYKNVLVNDLVLDKYGKK
                     MSKSRGNTVEPMDLFDRYGVDALRWYLLYVSPVWTPTRFDEDGLKEVLSKFIGTIKNV
                     YTFFALYANTDSIKPQDFFVEYKDRPELDRWIVSKYNNLVKYVTESMDVYDHNKSVRA
                     IQNFVNEDLSNWYIRRSRRRFWETGLTEDKKAVYNTTYEILVGISQLIAPFAPYLSEE
                     IYRNLTGEVSVHLSYYPKANEELIDENIEKRMDLVKDIVSLGRASRENAKIKVRQPIQ
                     KIHIDGKYQELIDDLVPLIMEELNVKEVVFEHDLNEFMDFTLKPNFKIAGPMLGNNVK
                     AFAGILAKADALEIITKVQSGEKYELDVNGQIIELDDQLLDIRIEAKEGYDVATGNNL
                     FTIIDTNLTEELINEGYAREFISKVQQMRKNNGYEMMDRIKIFYTSSPEIDKAVKEFE
                     DYIKEETLADLIDISNEEGMEVQNLNGIDTGMKLEKMN"
     gene            5095..6399
                     /locus_tag="PKH42_06505"
     CDS             5095..6399
                     /locus_tag="PKH42_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA20119.1"
                     /translation="MKNKIIIIGVVLIFLIAASYLIPQEVPMINIDDSIKMYIVADPH
                     YMSEKLTEDSEVFNNYLNTLDRMMKYTSVFLDIIEDDVKKNQPDIVLFPGDLTNNGSK
                     VNHLEFEEHLKRIKSTGAKVYVVPGNHDINNEKALYFKDNEIHITESINKEEFVEIYK
                     SYGYNEAISRDENTLSYLVKPYKNLWLLMLDTTKDYPEPGGYINRDTLKWIASCSDMA
                     KEENAEIIVVMHHNLLDHSDIIWEDYTVDNNTSVINTFHECDIQLVLSGHIHIQDVKT
                     HTSVETGNTIYDIATSSLPVFTHQYGQLTYSPSRGYDYETIKLNVEEYALKNNIEDEN
                     LLSFNEYAEKFFVENLCRTHKDSIGLLDIPDEHKEEVFKTVTEMNKKYFSGYRNEALK
                     QLIETEGFKIVEELDECFIQEYIMGMIKDQNADNNKLHIPIN"
     gene            complement(6396..7139)
                     /gene="aroD"
                     /locus_tag="PKH42_06510"
     CDS             complement(6396..7139)
                     /gene="aroD"
                     /locus_tag="PKH42_06510"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOA20120.1"
                     /translation="MENKRIKNINLNGMPKICVPVTGVTEHEIIDEINKLKNAPIDLV
                     ELRADYFEFVQDQTKVTDILQQIRKLYEKPLLFTLRTKNEGGVSYIDADYYFKLNNSV
                     IESKLVELIDIEFFSQAEKVDKTVKLARKNNVMTILSNHDFLKTPPEEEIISRVNKMM
                     DCGDIAKIAVMPNSEEDVLTLLSAALKLKKEKKGHFIAISMGPLGVISRISCELIGSC
                     ITYASYGDKSAPGQLDVRLVKELLEIMHV"
     gene            7463..9217
                     /gene="ptsP"
                     /locus_tag="PKH42_06515"
     CDS             7463..9217
                     /gene="ptsP"
                     /locus_tag="PKH42_06515"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOA20121.1"
                     /translation="MKGLGVSPGIGIGKAVVIDKASINITKNYIKDAGNELDRLMNAI
                     EAAKEQLNELYVSTIDELGEKEAQIFKSHEMMLEDDTFISDVENRIKQEGVNAEYALN
                     ETSNVYIKMFQSIEDEYLRERAEDIKDVMNRVIRILMGISSVDFSNLKENSIIVAKDL
                     TPSDTAQIDRTKVAAMITEIGGKTSHAAIIARIMGIPTVVGLNNITDKIKNGDTVICD
                     GKTGKVLVNPNAKQLFYFTKKKNIEDEIRNELKNQIGLPSVTKDGFKISLTANIGVPN
                     DVDMALENDAEGIGLFRSEFIFMNREHQPTEDEQFEQYKEVLVKMGDKPVIIRTLDIG
                     GDKNVPYIDIPKEMNPFLGYRAIRLCLGNVEVFRTQLRAILRASVYGNVKIMIPMIST
                     MKELKDSKKILQQAKDELTNEGIKFKKDIEIGIMIEIPSAAIISDLLAREVDFFSIGT
                     NDLIQYTMAVDRMNSKLSYLYSQYHPALLRLIKGIIDNAHEAGIWVGMCGEAAGDPKL
                     IPVFVGMGLDEFSMNSPSILRARYIVRNLNKADMELIAKSTLNMENALEVEEYLSCVF
                     ADENESCLRTCKNRRRQE"
     gene            9214..9795
                     /locus_tag="PKH42_06520"
     CDS             9214..9795
                     /locus_tag="PKH42_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOA20122.1"
                     /translation="MSKIVSIVFHSICGNNFLISKEFYKEFKAHNADVQLLRVKDINF
                     DELAKQFTIGTQYKKEILNIEEAKVEKLLDSDIIILGSPTYFGNVSGAMKAFMDEFYP
                     YWQSEKFYGKKMLAFTTCANGEGGGDLCLNAMTVFAQNMGMITVPVPKNLVSGQNFPA
                     NGLIHYVGDYSNLRPSGKIIPAIKEMTQKLMEM"
     gene            9928..>11234
                     /gene="rlmD"
                     /locus_tag="PKH42_06525"
     CDS             9928..>11234
                     /gene="rlmD"
                     /locus_tag="PKH42_06525"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOA20123.1"
                     /translation="MKKNDIIEVAINDLEFPNIGTTNFENKKIRIKNTLPGQKVRAKV
                     VRSRKESADARLIEVLENAYYEKEPLCEHFNLCGGCTYQTVPYKNQLEIKEKQVKNIL
                     DEILTEKYEFLPIVPSPKETEYRNKMEFSFGDEIKDGPLVLGMHKRNSFHGIVNTHGC
                     QIVDEDFRRILCHILEHFLGNSQTYYHKTTNIGFLRYLLVRKAEKTGEILINLITTSQ
                     STLNEKEFVDLILNIDLQGKVKCIAHSIFDGVADAAKADTMKVLYGEDRITEELLGLK
                     FNISSFSFFQTNSLGAEKLYSIVRDFIGNTKDKVIFDLYSGTGTIGQILAPAAKKVIG
                     IEIVEEAVKKANENVKLNNLTNCIFIAGDVLKKIDELNDKPDIIVLDPPREGIHPKAI
                     QKIIDYKPETFIYISCKPTSLKNDLPVFLDNGYKVSKVQCVDM"
BASE COUNT         3979 a         1744 c         2098 g         3313 t
ORIGIN      
        1 tttttgattt taattttgtc aattggaagc acggcaggac cttccaaaat atttcccttt
       61 atgtcaaacc tggagccgtg gcaagggcaa tcccatgttt tctcggcttc attaaatgca
      121 agcgcacatt ttaagtgtga gcaaacagga ttgatgcaaa aatattctcc tgcttcattt
      181 ctatatactc caaccttttt accgtcatag tcaactattt ttccttcgcc ggggttaact
      241 tttgaaagtt cttccaaatc ttcttctaat ctgttcaaaa atcctaaggc aatacttttc
      301 accgaagata caaattcttt tgatgattga acgggagtaa atctagaagg attaaaaata
      361 cctgaataat catcttcaat gcagagtatt ttgtttttta aaattaatgc agcagcaccg
      421 gaatgggaca ttccccattt tttaaatccg gttgctacgt ataaattatc tatactgtct
      481 gtatatctcc ctatatacgg aattttatcg taggtcatgc agtcttgagt agaccatttt
      541 gaaacaattt gcggattttt aaaattgctg ttcaaaaacc tttccaattc attatatgat
      601 tcgttttcat catccttttc accgacgcga tgattgcctc cagctaatat cagcagattc
      661 tccttgtctg aaaactggta tctcaaagaa taagtagggt cattattgtt aatgtacatt
      721 ccttcgaagg gtggttcatt tgttcttgct gcaataacat attctttttc ctgatacagt
      781 ctcaaaaaat aaagtccgaa gctattgtca aagggataat gtgatgcaac tattaccttg
      841 tctgcatgaa ttgttccact ttctgtttct acgtctacat gctcgtcgtg aggtacaatg
      901 tttaatgctc taacattttc atatatttga caattgcttt gatttaaaat attgtaaagg
      961 ctgtataaat attttaaagg attgaactga ccttggtttc ttacgcctat tgcgccgtaa
     1021 gctttatttg gaataggaac agtatctgta taaaatccgt ctatacctaa tttttcatag
     1081 gcttttaact cttttttcaa atcttcaact tcattaacgg atgctgcata aacatatgcg
     1141 gtctgttctt tgtagtcgca tttaatgttg ttttcgttaa taattctttt tattaatctt
     1201 attccttctt cattggcttt taaatattgc tttgcttctt ccatgctgaa gctgtttatt
     1261 aaatagtcgt aaaacaaatc gtgctgtata gtaatttttg ctgtagtata gcctgtaacg
     1321 ccgtatccgg attttgtggc ttcaataatt ccaatgttga acccgcagtc ttttaataaa
     1381 tatgcggtag tcagtcccgt aatcccggca ccaataatta aaatgtcgaa ttttttgttt
     1441 tttaaatccg cattttctac agctggtttt tttattcctt cagctgtatc atgccaatat
     1501 gaacgtcttg gatattccat agtaacctct tttcataata tttttcaata atattagtgt
     1561 tattgtttca taaatatttc aatttattcc ttgactttgt tcatattttc atatatcata
     1621 atacagaata aaatcaatat caaaaacagt gattggaaga gtaattgcag taggattcaa
     1681 agagatataa tcgattggtg tgagattata aatctgaatg caacgaagat catccaggag
     1741 tttaatttct gaaataccag taagaaatta cgtacaaatg cgttaaattt tcgagtgaca
     1801 tatgctatta ttgtatatgt aatttgggtg gtaccgcgaa attcgtccca tgtctattga
     1861 catgggactt tttaatggca aacaaattgg tgatggagcc tgtcctgaca tcaattaaaa
     1921 tataatacga ttaacatata ggagggaaat atgaaattat ttaaagatat atcaaaagct
     1981 gattataaaa ataatgagca tgaagtatcg aaatattggg aagaaataaa cctattggaa
     2041 caaagcattg aaacaagaaa aaacaaacca tcatttgttt tttatgaagg tcctcccaca
     2101 gccaacggga gacccggaat acatcacgtt atctcaagga cattgaaaga ctctgtctgc
     2161 agatacaaga ctatgcaagg atatcaggtt aaaagaaaag cagggtggga cactcacgga
     2221 cttcctgttg aaattgaagt tgaaaaacaa ttaaacttaa gtaccaaaca ggaaatcgaa
     2281 gaatacggaa ttgacgaatt taataaaaaa tgtcgtgaat cggtattcac ctacgaagca
     2341 ttatggcgtg aaatgactga gcgtatggct tatttgattg atttggataa tccttatatt
     2401 actcttgaaa atgactacat tgaatccgaa tggtggatat tggataaagt tttcaaggag
     2461 ggattaatat atgaaggaca caaaattctt ccgtattgtc caaggtgcgg aacaggtctt
     2521 gcttctcacg aggtagcaca aggctatgaa gaaattaaaa caataactgc ctatgcgaaa
     2581 tttaaaaaga aggatgaaga tgcatatttt cttgcatgga ccacaacgcc atggacttta
     2641 ttatccaatg ttacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggtatt
     2761 gttcacactg cacctgcttt tggtgaagat gattacaaca cgggattaaa atacaatttg
     2821 cctgttttaa atccggttga tgatacagga aggttcagag gtacaccttg gtcagaaatg
     2881 tttgtaatgg atgcagacca gccgatatta agatggttga aggaaaacgg gaagttatat
     2941 aaaaaagaac cggttcttca taattatccg cactgctgga gatgccacac accgctgctt
     3001 tactatgcaa gaccaagctg gtatattcaa atgacaaaat taaaggattt gcttgttgca
     3061 aataacaaca cggttaattg gtatccagat tatgtcggtg aaaagcgttt tggcaactgg
     3121 cttgaaaatg ttaatgactg ggctatttcg agaagccgtt attggggaac acctcttccg
     3181 atttggagat gcgaatgcgg tcatatcgaa agtatcggtt caagaaaaga attagttgaa
     3241 aaagcagttg aagatataga tgaatcaatt gagcttcaca gaccatacgt tgatgaggtg
     3301 catattgttt gtcctgaatg cggaaagcat atgacaagag taaaagacgt tatcgactgc
     3361 tggttcgatt caggtgccat gccttttgca caatggcatt atccgtttga aaacaaagaa
     3421 agatttgatg aattgttccc tgctgatttt attaatgaag gtattgacca gaccagagga
     3481 tggttctatt cattaattgc cgtatcgacc ctggttacgg gtaagtcacc atataagaat
     3541 gtattggtaa acgacttggt tcttgataag tatggaaaga aaatgtcaaa atcaagaggc
     3601 aatacagtag agcctatgga ccttttcgat agatacggtg ttgacgcact aagatggtac
     3661 ctcttgtacg tgtcgcccgt atggacacct actcgttttg atgaagacgg gttaaaagag
     3721 gtattgagca agtttatagg aacaattaaa aatgtatata ctttctttgc tttatatgca
     3781 aacactgatt caatcaaacc gcaagacttc tttgttgaat acaaggatag accggaactg
     3841 gacagatgga ttgtatcaaa atataacaac ctggtaaaat atgtgacaga aagtatggat
     3901 gtttatgacc ataacaaatc tgtaagagca attcagaatt ttgttaatga agatttgtca
     3961 aactggtaca ttagacgttc aagaagaaga ttctgggaga ctggacttac tgaagataaa
     4021 aaggcggttt ataacaccac atatgaaata ttggttggca tttctcaact gattgctcca
     4081 tttgctcctt atttgtcaga agaaatttac aggaatttaa caggcgaagt atcagtccat
     4141 ttaagctatt atcctaaggc aaacgaggag ctgatagacg aaaatattga aaagagaatg
     4201 gatttggtaa aagatattgt aagcttagga agagcatcca gagaaaatgc taaaattaag
     4261 gttagacaac cgattcaaaa aattcatatt gacggaaaat accaggaatt aattgacgat
     4321 ttggttccat taatcatgga agaattaaat gttaaggaag ttgtatttga gcatgactta
     4381 aatgaattta tggatttcac cttgaaacct aattttaaga ttgcaggacc tatgcttgga
     4441 aacaatgtaa aggcatttgc cggtattttg gcaaaggcag atgcattaga aataattaca
     4501 aaagttcagt caggcgaaaa gtatgaattg gatgtcaacg gtcaaataat tgagcttgat
     4561 gaccaattgc tggatatcag aattgaggca aaagaaggtt atgatgttgc aacaggaaac
     4621 aatttattta ccataattga tactaatctt acggaagaat tgataaacga aggatatgcc
     4681 cgtgaattta tatccaaagt acagcaaatg agaaagaaca acggatatga gatgatggac
     4741 agaataaaaa tattctacac aagctcacct gaaattgaca aggccgtaaa agaatttgaa
     4801 gattatatca aggaagaaac actggcagac ttaattgaca tatcaaatga agaaggtatg
     4861 gaagttcaaa acctgaacgg tattgataca ggaatgaaat tagaaaaaat gaattaatac
     4921 tcaaaaacag tatggctgat tcatacttaa tataggaatg gagcactcca taatagagga
     4981 gtgttccaat ttttatgcca ttttattggc aaactaaaaa ataaaaacat tgttattttt
     5041 ttgattttat tgtataatga aataaataga attatataac cggagggtgg agcaatgaag
     5101 aataagatta tcataatagg agttgtttta atatttttaa ttgctgcatc ctacctcatc
     5161 cctcaggaag tacctatgat taatattgat gatagtatta agatgtacat agttgctgac
     5221 cctcattata tgtctgaaaa attgacagaa gatagcgaag tatttaacaa ctatttgaat
     5281 accctcgata gaatgatgaa atataccagt gttttccttg atatcataga agatgatgtt
     5341 aaaaagaatc agcccgatat tgttcttttt ccgggagatt taacaaataa cggctccaag
     5401 gttaatcact tggaatttga agagcattta aaaaggataa aatcaacggg tgcaaaggta
     5461 tatgtagttc cgggaaacca tgatattaat aatgaaaaag cattatactt caaagataat
     5521 gaaatacaca taactgaaag tataaacaaa gaagagtttg tggaaatata taaatcatac
     5581 ggatataatg aagcaatatc aagagacgaa aatactctca gctacttagt taagccttac
     5641 aagaatttgt ggctgttaat gcttgatacg acaaaggatt acccggaacc gggcggttat
     5701 ataaacagag atacactaaa atggattgca tcatgttcag atatggcaaa ggaagaaaat
     5761 gcagaaataa tagttgtaat gcaccacaac ttgcttgacc acagcgatat tatatgggaa
     5821 gactatacag tggacaacaa caccagcgta attaatacat tccatgaatg cgatatacag
     5881 cttgtgctgt caggacatat acatattcaa gatgttaaaa cacatacatc cgtagaaaca
     5941 ggcaatacaa tatatgatat agctacaagc agcctgccag tatttactca ccagtacgga
     6001 cagttgacct attctccgtc aagaggctat gattatgaaa caatcaagct gaatgtggaa
     6061 gagtatgctt taaaaaataa tatcgaagat gagaatttac tttcatttaa tgaatatgcc
     6121 gagaagtttt tcgttgaaaa tttatgcaga acacataaag atagtatagg tctgttggac
     6181 attcccgacg agcataaaga agaagtattc aaaacggtaa ccgaaatgaa caaaaaatac
     6241 ttctcaggtt acagaaatga ggcattgaag cagcttatag aaaccgaagg gtttaaaatc
     6301 gttgaagaat tagatgaatg cttcatacaa gaatatataa tgggaatgat taaggaccaa
     6361 aatgcagaca acaataaatt gcatattccc ataaactaaa catgcatgat ttctaacagc
     6421 tctttaacta gtctgacatc taattgaccc ggtgccgact tatcgccata agaagcatag
     6481 gttatgcagg aaccgattaa ttcacatgat attcttgaaa taactccaag aggccccata
     6541 gaaatggcta tgaaatggcc ttttttttct ttctttaatt taagggctgc agaaagcagt
     6601 gtcagtacat cttcttcaga attaggcatc actgcaattt tggcaatatc tccgcaatcc
     6661 atcatcttat taacccttga gattatttct tcctccggag gagtttttaa aaaatcatgg
     6721 ttggacaata ttgtcatcac attatttttt cttgctaatt taacggtttt atctactttt
     6781 tcggcttgag agaaaaattc aatatctata agctcaacca acttgctttc gattacactg
     6841 ttgtttagtt taaagtaata gtcagcatct atgtatgaaa ccccgccttc attttttgtt
     6901 cgcaatgtaa acagaagagg tttttcatat aattttctta tttgctgaag aatatctgtt
     6961 acctttgtct ggtcctgtac aaattcaaaa taatctgctc taagctcaac caaatctatg
     7021 ggagcatttt ttagcttgtt aatttcatca attatttcat gctctgtaac accggttacc
     7081 ggcacacaaa ttttcggcat accatttaaa tttatatttt taattcgttt attctccact
     7141 ttaattctcc aaattttttt tatttatttt atcattaatg caagttacta ttggcttgct
     7201 tcacattgat actattatac aattgtgcaa aggcaaggtc tttgctcatc gattgtacta
     7261 ttatataata agaattaaag aaagacaaca atgaaaacat tgtcccaaaa aatgttgaaa
     7321 ttgcaatatt tttaattgac aatgcattgc atatagtact ataattatat aaattgggag
     7381 caatgcaata aaaagattac taaatttata taaggtttgt caatataaat ttagtattaa
     7441 aagactgaaa ggtagtgaag ttgtgaaagg attgggagtg tcaccgggta ttggtatagg
     7501 taaggccgta gtaattgata aggcatcaat taatataacc aagaattata ttaaagatgc
     7561 cggaaatgaa ttagacaggc ttatgaatgc cattgaggca gcaaaagagc agcttaacga
     7621 attgtatgtt tcaacaattg atgaattagg tgaaaaagaa gctcagattt ttaaatctca
     7681 cgaaatgatg ttggaagatg atacatttat ttcagatgta gaaaacagaa taaaacaaga
     7741 aggtgtaaat gccgaatatg ctttgaatga aacttccaat gtatatatta aaatgtttca
     7801 aagcattgaa gacgaatacc tcagggaaag agcagaagat atcaaagatg taatgaatag
     7861 agtcataaga atactgatgg gtatttcaag tgttgatttt tccaatctga aagaaaactc
     7921 tatcattgtt gcaaaagatt tgactccctc cgacactgca caaatagaca gaacaaaagt
     7981 agcagccatg attacggaaa taggaggcaa gacatcccat gctgcaataa ttgcacgaat
     8041 catgggcatc cctactgttg taggtttaaa caatataaca gataaaataa aaaacggcga
     8101 tactgtaatc tgcgatggga agacaggtaa agtgctggta aatcccaatg caaaacagct
     8161 tttttatttt actaagaaaa aaaatataga agatgaaatt agaaatgaat taaaaaatca
     8221 aataggactg ccttcagtaa caaaagatgg atttaaaata tctttgacag caaatatagg
     8281 tgttccgaat gatgtagata tggcattaga aaatgatgca gaaggaatcg ggttattcag
     8341 aagcgaattt atatttatga atagagaaca tcaacctact gaagacgaac aatttgagca
     8401 atataaggaa gtccttgtta aaatggggga taagcctgta ataataagaa cattggacat
     8461 cggcggggac aagaatgtac catatatcga tattccaaaa gaaatgaatc ctttcttggg
     8521 atacagggca ataaggctgt gtcttggcaa tgtggaagtt ttcagaacac agctgagagc
     8581 aattttaagg gccagtgttt acggcaatgt taaaataatg attcctatga tttccaccat
     8641 gaaggaatta aaagactcaa agaaaatact gcagcaagca aaagatgaat taacaaatga
     8701 aggcataaaa ttcaaaaaag atatagaaat aggtatcatg attgaaatac cttctgctgc
     8761 aataatatct gaccttttag ccagggaggt tgactttttc agtattggaa ccaatgattt
     8821 gattcaatat accatggctg ttgacaggat gaattccaaa ttatcatatt tgtacagcca
     8881 ataccacccg gcacttttaa gactaataaa gggtataatt gacaatgcac atgaagccgg
     8941 catctgggtc ggcatgtgtg gtgaagcagc cggagacccc aagcttatac cggttttcgt
     9001 gggaatggga ctagacgaat tcagcatgaa ttctccttcg attctcaggg caagatatat
     9061 agtgagaaat ttaaataaag cagatatgga gttaatagca aaaagtaccc tcaatatgga
     9121 aaacgcactt gaggtagagg agtatctgag ctgcgtgttt gcggatgaaa acgaaagctg
     9181 cttaagaact tgcaaaaata ggagaagaca agaatgagca aaatagtatc aatcgtattt
     9241 cacagtattt gcggaaataa ctttctcata tcgaaagaat tttataaaga attcaaagct
     9301 cacaatgcag atgtgcaatt gttaagagta aaggatataa actttgatga acttgcaaag
     9361 caatttacaa taggcactca atataaaaaa gaaatattga atatagaaga agcaaaagta
     9421 gaaaagcttc ttgacagcga tataataata cttggttcac ccacatattt tgggaatgtt
     9481 tcaggtgcca tgaaagcatt catggatgaa ttttatccct attggcagag tgagaagttc
     9541 tatggcaaaa agatgttagc ctttacaacc tgtgccaacg gagaaggcgg aggggactta
     9601 tgcttgaatg caatgactgt ctttgctcag aatatgggaa tgattacagt gccggttccc
     9661 aaaaacttag tctccggtca gaattttccg gcaaacggat taattcatta tgtaggagat
     9721 tattcaaatc taagaccaag cggtaaaata attcctgcaa taaaagaaat gacacaaaag
     9781 cttatggaaa tgtaaattat tataaatggg gacattcctt ttatcttgac agaaggtgtg
     9841 tccccatgtc attatgagac cattagctcg aagaatctca tacattgtca ttctgaacgc
     9901 agtgaagaat ctaatcaagg agtacaaatg aaaaaaaacg atataattga agttgcaatt
     9961 aatgatttag agtttcctaa tataggaaca acaaattttg aaaataaaaa aataagaata
    10021 aaaaacactt tacccggaca aaaggtaagg gctaaggttg tacgttcacg aaaagaaagt
    10081 gcagatgcaa gactaataga agtattagaa aatgcatatt atgagaaaga gccgctctgt
    10141 gagcatttta acttatgcgg gggctgtacc tatcaaactg taccatacaa gaaccagctt
    10201 gaaataaaag aaaaacaggt taaaaatata ttagatgaaa tattgacaga aaaatatgaa
    10261 tttctaccta tagttccaag tcctaaggaa actgaatata ggaataaaat ggagttttca
    10321 ttcggcgacg aaataaaaga tggcccttta gtattgggaa tgcataaaag aaacagcttt
    10381 cacggcatag ttaataccca tggatgccaa attgtagatg aagactttcg acgaatatta
    10441 tgtcatatat tagagcattt cctcggaaat agtcaaactt attatcacaa aaccactaat
    10501 attggctttt taagatactt attagtgaga aaggcagaaa aaacaggaga gattttaata
    10561 aatttaataa caaccagtca aagtacatta aatgaaaaag aatttgttga cttgatttta
    10621 aatatagacc tgcaaggcaa agtaaaatgt attgcacact cgatatttga cggagttgca
    10681 gatgcagcaa aagccgacac tatgaaagtt ctctatggtg aagacagaat tacagaagag
    10741 ttgttgggac ttaaattcaa catttcatca ttttcttttt tccaaacaaa ttctttggga
    10801 gcagaaaaac tatactcaat cgtaagagac ttcataggca atacaaaaga taaagtaata
    10861 tttgacctat attccggaac cggtaccatt gggcaaatat tagctcctgc agcaaaaaag
    10921 gtaataggaa tagaaatagt cgaagaagca gtaaaaaaag caaacgaaaa tgtgaaactt
    10981 aacaatttga ccaactgcat tttcatagca ggagacgtac ttaagaaaat agatgaacta
    11041 aatgacaaac ctgatataat agtccttgac cctccccgtg aaggtattca tccaaaagca
    11101 atccaaaaaa taatcgacta taaaccggaa acgttcatct acatttcatg caaaccaaca
    11161 tccttaaaaa atgacctacc tgtcttttta gacaacggct acaaggtcag caaagttcaa
    11221 tgcgtcgata tgtt
//
LOCUS       DAOPUE010000061        10534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6367_length_10534_cov_8.841588, whole genome shotgun sequence.
ACCESSION   DAOPUE010000061 DAOPUE010000000
VERSION     DAOPUE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6367_length_10534_cov_8.841588"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKH42_06530"
     CDS             <1..252
                     /locus_tag="PKH42_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20124.1"
                     /translation="LDEPSLGLAPLLINTIFDIIIEIKKMGKTILLVEQNAFKALSVA
                     DKAYVLEQGAIVMEGPASDLIKNERIQEAYLGKKKSDVG"
     gene            361..1035
                     /locus_tag="PKH42_06535"
     CDS             361..1035
                     /locus_tag="PKH42_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOA20125.1"
                     /translation="MKLTFLGHSALLIEEGNFKGLVDPFITGNSLSKISVDDLVNITH
                     IFVTHGHGDHIGDTINIAKRNKALIITNHEISAYLSKFKLRTHAMHIGGRTKFDFGTV
                     KMTNALHGSGISENGNMIYGGNPGGFIIEVNGKKIYHAGDTGLTYDMKLLEDEKIDVA
                     FLPIGGNYTMDIEDALKAVDFIKPKTVVPMHYNTFGIINADPEIFKSKASSNVIILNV
                     NESMNV"
     gene            1102..1455
                     /locus_tag="PKH42_06540"
     CDS             1102..1455
                     /locus_tag="PKH42_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20126.1"
                     /translation="MFALVLILNDIYKLDEINELFYNEELGATSIDSQGIGKVLLDHN
                     INLPILDSVKKLIDGRKPYSKIIFSVIKDREKLDVVIKKLREELDYFEEPGTGFMFVI
                     PVIEVYSHSTENTEF"
     gene            1683..1916
                     /locus_tag="PKH42_06545"
     CDS             1683..1916
                     /locus_tag="PKH42_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOA20127.1"
                     /translation="MTLDKIKPGQGGVIASIGGQGLLRRRLLDMGLTPNTCVKVRKVA
                     PMGDPIELSLRSYILTIRKDEAAKIELKEVFDI"
     gene            1913..3925
                     /gene="feoB"
                     /locus_tag="PKH42_06550"
     CDS             1913..3925
                     /gene="feoB"
                     /locus_tag="PKH42_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOA20128.1"
                     /translation="MSVIALAGNQNSGKTTLYNKLTGSNQHVGNFPGVTIEKKEGQLK
                     KHKDISIVDLPGIYSLSPYTMEEVVTRDFLLKEKPDLILNIVDATNIERNLYLTLQLM
                     ELSIPMVIALNMMDEVTASGNSIDVQKLSSHLGIPVVPISASKGEGISELNDVVIRSI
                     KEKPIPKRHDFCVGEVHKAIHSISHLIADQAEKAGYPPRFAATKLVEGDEPTFKSLNI
                     TENQMHIIEHVVKDMEEALKTDREAALADMRYCYIEKICSESVIKKQETKEQQRSEKI
                     DEILTHKYFGIPIFFGIMLFIFWMTFNVIGAPLQEFLEVVIALLTETISNALINIGVS
                     DWVYSLVINGIFVGVGSVLSFLPIIVVLFFFLSLLEDSGYMARVAFLMDKLLRKIGLS
                     GRSFVPMLIGFGCSVPAIMATRTLASERDRKMTIVLTPFMSCSAKLPIYGMITAAFFP
                     DNAALVMIGLYVMGIAVAVLSGLLLKNTIFKGEPVPFVMELPAYRIPSRKSVLIHMWE
                     KAKDFIQRAFTIIFLASIVIWFLQGFNWSFEMVEDSSKSILAYLGSMISPIFRPLGFN
                     DWRASTALITGLTAKEAVVSTLSVLTGAATDAQLLEALFIIFTPISAFAFLTFVLLYM
                     PCVAAFAATKRELGSLKYAVLTALYQTVTAYTAGAIVYQIGSFIGG"
     gene            3928..4086
                     /locus_tag="PKH42_06555"
     CDS             3928..4086
                     /locus_tag="PKH42_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOA20129.1"
                     /translation="MNFADGVILFIIAAIVAAVIKVIKKQNKNNGGCSGCSGCSKANS
                     CPSAKKLS"
     gene            4164..4787
                     /locus_tag="PKH42_06560"
     CDS             4164..4787
                     /locus_tag="PKH42_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20130.1"
                     /translation="MNEINDLRLRDWTRDFLVQRIFGRGRNLMTAENAIIENINIADR
                     TVYVTISYGVLGDFNVINMELVRLVVDRDTVILDQFGRPMSSRELREGMRVDAVFSAA
                     MTRSIPPQARAYSIIVIDDGMQSSFTEDTVIEVDLANRFLYTGIPFDILSQMRFVVTD
                     STTIRDRRGRRIRLRDLRPGDFVRIEHANFMTMSIPPQTTAYDIQVL"
     gene            complement(4877..6943)
                     /locus_tag="PKH42_06565"
     CDS             complement(4877..6943)
                     /locus_tag="PKH42_06565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA20131.1"
                     /translation="MVSSSLNKGLSIITYSKKHMENVIAQLTNLLGDEISIKGYSIQE
                     GINEEICDSLVLICHKYLKEEALKHITPGANVLVARRSLNHSKLEDILQIPEGEKVLF
                     VDVNKYLAESSISLLVELGINHISMYPYYPGQEFPEYADYSITTGETHLVPDYVKNII
                     DIGYSKIDLTTITEIMIKLGIMDERANLLSSRYLQDFAYSSKKIVETLSDNKHITEQL
                     NTILNVINDGIVGIDGSGEIMFSNNNANKLFNNTEINLNDINNFDIKSFQGKTFDVYR
                     EIHEIKGKKVLLTMMPIIEDNANNGIIITFKDLSEIMEMENIVRVNLFKKGHTAKYRF
                     SDIVGKSKNMSETIEIAKKLSRSNSTLLLTGESGTGKELFAHSIHNASKQQYGPFVAA
                     NFAALTESLLESELFGYEEGAFTGARKGGHAGLFEQAHKGTIFLDEIGDASPRIQARL
                     LRVLQEKEVLRVGGTKIIPIDVRVIASTNKDLHKLVQKGDFREDLYYRLNVLPINIPP
                     LRERKSDIPLLIKMFLKKFPNGSEMVISNEVMAKFLEYDWPGNVRELENVIEYLYNLA
                     GSKIELKDLPLDLAGGKKQEDIQTQNQQILEKTLKDLEMEIPLQDVLTILNAIKRSKD
                     EGYAYIGRKKLAELVKDEFTEQMIRSRLEILSQAGLIITERGPKGSRLSLKGEKFLDL
                     INNTLE"
     gene            complement(7028..>8376)
                     /locus_tag="PKH42_06570"
     CDS             complement(7028..>8376)
                     /locus_tag="PKH42_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOA20132.1"
                     /translation="IFSTDGQKIFGAQIIGEDGVDKRIDTIATTIGLKGSIYDLKELE
                     LAYAPPYSSAKDPVNMLGFVAENIIQNKVKFSKWNDIDPSKQNEYTLLDIREEAEHMV
                     FDIPGTVNIPLGQLRGRINELNKDDNIIILCSIGVRSYNAARVLMENGFKNVLVYPAG
                     TNFYKSVYYNYFNETGRESNDDTLVVSNTNEKKTDTTVKIKSSIQVDCSGLQCPGPIM
                     KVYETMKNANDGDIVQVSATDSGFAKDVEAWSRRTGNTLLKIEKDGKNNIVYLQKGID
                     NKKAETALEKTGLQPSNDGKTIIVFSGDMDKVLASFVIANGAAAMGRPVTMFFTFWGL
                     NVLRKTEKQNIKKPFIDTMFGKMMPRGVNKLKLSKMNMGGLGTAMMRKVMKDKNIDSL
                     EDLIKKAMANGVKMIACTMSMDVMGITKEELIDGVEFAGVGTYLGDAEESNVNLFI"
     assembly_gap    8377..8476
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8477..9363)
                     /locus_tag="PKH42_06575"
     CDS             complement(<8477..9363)
                     /locus_tag="PKH42_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA20133.1"
                     /translation="MSKKILIIGGVAGGASAAARLRRLDETSKIIMFEKGPYISYANC
                     GLPYHIGKVIKERSSLLLQTPEAMSKKYNIDVRVESEVISINRESKTLDVKNLKTGEI
                     YTESYDTLIISTGSSPLKPPIEGINSPGIFTLWTVPDTDNIIKFINEKKPKNALVIGG
                     GFIGLEVAENLAAAGLNVTVAEAMNQVMAPIDYEMAQLVHENMVSNNIELILGDGVSK
                     FESNDGVITITLASGRTLTADMVILSIGVRPNGQLAKEADLEVNQRGGIIVDKYLKTS
                     DPSIYAIGDVIEVEEFINKG"
     gene            complement(9374..9673)
                     /locus_tag="PKH42_06580"
     CDS             complement(9374..9673)
                     /locus_tag="PKH42_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOA20134.1"
                     /translation="MIDFSNKERVKKVLSEKAELFKSIAHPVRLCILAMLIKEEESNV
                     TDIQCCLDVPQPTVSQHLSKLKSAGILSAERNGTEIYYKIINEEVKKIVSQILDA"
     gene            9825..10154
                     /locus_tag="PKH42_06585"
     CDS             9825..10154
                     /locus_tag="PKH42_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA20135.1"
                     /translation="MEIITSYENLQELIEKNDMVMIYFGSKTCSVCKSMTPKVENMIK
                     RYPNIYAIMVEADKSLDISSKFNVFTVPVILLYIEGKETIREVRIISLDNLEQKIARY
                     CQIFYVH"
     gene            complement(10220..>10534)
                     /locus_tag="PKH42_06590"
     CDS             complement(10220..>10534)
                     /locus_tag="PKH42_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOA20136.1"
                     /translation="SFYYEWKQEEKGKHKYKINKPGSNIYMAGLYNIAANKNKQLSLF
                     DENIDIYYTIITRNSNNSISHIHSRMPLIFSIEEMNEWLRGQSIDELMKSEHMLTGEI
                     TE"
BASE COUNT         3248 a         1740 c         1891 g         3555 t
ORIGIN      
        1 cttgatgaac cttcattagg gcttgcacct ttattgataa atactatatt tgatattata
       61 attgaaatca aaaagatggg aaaaaccatt cttttggtag agcaaaatgc atttaaggca
      121 ttgtctgttg ccgataaggc atatgttttg gagcaaggtg caatagtaat ggaaggacct
      181 gcttcagatt taattaaaaa tgaaagaata caggaagcat atttaggaaa gaaaaaatca
      241 gatgtcggtt agccaaatta agtgtcattt ttggaaatct catgtgagat ttcttttttt
      301 agttgatttt attataatat aaagatatta tatacttaat aataaaaaag aggtgattat
      361 atgaagctta ctttcttggg acattcggca ttattaattg aggaaggaaa tttcaaggga
      421 cttgtggacc cctttataac aggaaattct ctgagtaaaa taagtgttga tgatttagtg
      481 aatataaccc atatatttgt aactcatggg catggtgatc atataggaga tacaattaat
      541 atagcgaaaa gaaataaagc attgataatt actaatcacg aaatttctgc ttatttgagc
      601 aagtttaaat taagaactca tgcaatgcat atcggcggca ggacaaagtt tgattttgga
      661 acggtaaaaa tgacaaatgc tctgcacggc tccggtattt ccgaaaacgg aaatatgatt
      721 tacggaggca atccgggagg atttattatt gaggtaaacg gtaaaaagat ttaccatgct
      781 ggtgatacag ggcttacata tgatatgaaa cttttggagg atgaaaaaat agatgttgcc
      841 tttctgccta taggtggcaa ttataccatg gatattgagg atgcccttaa ggcagtagat
      901 tttataaaac caaagaccgt ggttccaatg cattataaca catttggcat cattaatgct
      961 gaccctgaaa tatttaagag taaagcatca tcgaatgtaa taatattgaa tgttaatgaa
     1021 agcatgaatg tgtaaatgat tgttgaatag cagaaaactc atatggtata ataacaatat
     1081 catcagaacc agggaggtta tatgtttgct ttagtgttga tattaaatga tatttataag
     1141 cttgatgaaa taaatgaatt gttttataat gaagaattag gagctacttc tatagacagt
     1201 caggggatag gcaaggttct cttagaccat aatataaatt taccgatact tgacagcgta
     1261 aaaaagttga tagacggcag aaaaccctac agtaaaataa tattcagcgt aataaaagat
     1321 agagaaaaac ttgatgttgt tattaaaaaa ttaagggaag aattggatta tttcgaagaa
     1381 ccgggcacag gctttatgtt tgtaatacct gttatagagg tttatagcca cagtacggaa
     1441 aatactgaat tttaaaatta ataaataata agaatatgat gttatcgaag gggagcatac
     1501 cttcaagatc cgataattcg tggatgtatt aaagatatcg catggattgc ggataatttt
     1561 tgaaggaagg tccccgtata tgtcatattt tttttaattt ttgttgacaa ttgctatcaa
     1621 tgggggtata attaaaatat ctaaatgata atgattatca atatcgaaat tggaggtgcg
     1681 atatgacatt agataaaata aaaccgggtc aaggcggggt cattgcatca ataggcgggc
     1741 aaggtttatt gagaagaaga cttcttgaca tgggtttgac acctaatacc tgtgtgaagg
     1801 tcaggaaggt tgcacccatg ggtgatccca tagaattatc tttgagaagc tatatattga
     1861 caatacgaaa ggatgaagca gcaaaaatag agctgaaaga ggtatttgac atatgagtgt
     1921 tattgctctt gcaggaaatc agaattccgg aaaaactact ttatacaaca aactgacagg
     1981 aagcaatcaa catgtcggta attttcccgg tgtgacaata gaaaaaaaag aaggtcaatt
     2041 gaaaaaacac aaggatattt ccatagtgga tttgccgggg atttattctt tgtccccata
     2101 tacaatggaa gaagttgtta ccagggattt tttacttaag gagaaacctg atttaatatt
     2161 aaatatagtt gatgcaacaa atattgaaag aaatttatac ttgacactcc agcttatgga
     2221 gttatcaatt cctatggtca ttgcactgaa tatgatggat gaagttacag ccagcgggaa
     2281 ttccatagat gtacaaaaat tatcctctca tttgggaata cctgtagttc ccatatctgc
     2341 aagcaaaggt gaaggaatat ctgagctgaa tgatgttgtc ataaggtcaa ttaaggagaa
     2401 accaattcct aaaaggcatg atttttgtgt aggtgaagtg cataaagcaa tacattccat
     2461 ttcacactta atagctgacc aggcagaaaa agcaggttat cctccgaggt ttgccgcaac
     2521 taagcttgtt gaaggagatg agcctacttt taagagcttg aatattactg aaaatcaaat
     2581 gcatattata gagcatgtag ttaaagatat ggaggaagct ttaaaaaccg acagagaagc
     2641 agccttagca gacatgagat attgctatat agaaaagatt tgctcggagt cagttattaa
     2701 aaagcaggaa acaaaagagc agcagcgctc cgaaaaaatc gatgaaatat tgacacataa
     2761 atattttggt atacctatat tctttgggat aatgcttttt attttttgga tgacatttaa
     2821 tgttataggg gctcctcttc aggaattttt ggaagttgta attgctttat taactgaaac
     2881 aatatcaaat gcattgatta atataggggt tagcgattgg gtatattcac ttgtaattaa
     2941 cggtatattt gtcggcgttg gaagtgtatt gtccttcctt cccattattg tagtcttatt
     3001 cttttttttg tcccttttgg aagacagcgg ctatatggca agagtagcat ttttgatgga
     3061 taagcttcta agaaaaatcg ggttgtcggg aaggtctttt gtcccaatgc ttatcggctt
     3121 tggatgcagt gttcctgcaa ttatggcaac aaggaccttg gcaagcgaaa gggacagaaa
     3181 aatgacaatt gttttaacgc cttttatgtc ttgcagtgcc aagctaccta tttatggaat
     3241 gattactgca gcattctttc ctgataatgc tgcattggtt atgattggct tgtatgtaat
     3301 gggcattgca gttgccgtat tatcgggttt gttattgaaa aatactatat ttaagggaga
     3361 gccggtgcca tttgtaatgg aactgcctgc atatagaata ccttcaagaa aaagtgttct
     3421 gattcatatg tgggaaaagg ctaaggactt tattcaaaga gcatttacaa taatattctt
     3481 ggcttctata gtgatatggt ttttgcaagg atttaactgg tcctttgaaa tggttgaaga
     3541 cagctcaaaa agcatacttg cttatttagg ctccatgatt tcaccgattt ttagaccatt
     3601 gggatttaat gactggagag cttcaacggc acttattaca ggattaacag ccaaggaagc
     3661 tgttgtaagt actttgagcg tattgacggg agccgcaaca gacgcacagc ttttagaagc
     3721 tttgtttata atatttacgc ctattagcgc atttgcattt ttaacatttg ttttattgta
     3781 catgccctgt gttgctgctt ttgcggcaac aaaaagggag ttgggctcgt taaagtatgc
     3841 tgtattgact gccttatatc agacggttac cgcatataca gcaggagcta ttgtttatca
     3901 aataggaagc tttataggag gctgataatg aattttgccg atggagtaat tctttttatt
     3961 attgcagcaa ttgttgcagc agtaatcaaa gttataaaaa aacaaaataa aaataatggc
     4021 ggctgctcgg gctgctccgg atgcagcaaa gcaaacagct gtccttctgc aaaaaaactg
     4081 tcctgattga gacagttttt ttatcaataa catatttata tcttctgaat atatataata
     4141 atatatatat gcgaggtgaa aatatgaatg aaataaatga tctaagatta agagattgga
     4201 ccagagattt tttagtacaa agaattttcg gaagaggcag aaatttaatg acagcagaaa
     4261 acgctattat tgaaaatata aatatagctg acagaacagt ctatgtaacg atatcatatg
     4321 gtgtattggg agattttaat gtaataaaca tggagttagt cagattggtt gttgacagag
     4381 atactgttat attggatcaa tttggtcgtc ctatgtcaag cagagaatta agagaaggaa
     4441 tgagggtgga tgctgtattt tcggctgcta tgacgagaag tatacctcct caggcgagag
     4501 cctacagcat aattgttatt gatgatggta tgcaatcaag ttttactgaa gatacagtca
     4561 tagaagttga tttagcaaat agatttttgt atacagggat tccgtttgat atattgagcc
     4621 aaatgaggtt tgtagtaacg gattcaacaa ccatcagaga taggagagga agaagaataa
     4681 gattaagaga tttaagaccg ggcgattttg taagaattga acatgcaaat ttcatgacaa
     4741 tgagcatacc gcctcaaaca actgcgtacg atatacaagt tctttagata aataactatc
     4801 atgagttatg aagaaaacta acaattataa gattaaaggg agcagaagtt ctgctccctt
     4861 tttcaagaat tccggattat tctaacgtgt tgtttatcaa atctaagaat ttctctcctt
     4921 ttaacgataa tcttgaacct ttaggtcctc tttcggtaat tattaatcct gcctgtgata
     4981 gtatttcaag acggcttcta atcatctgct cagtaaattc atctttcact agctctgcca
     5041 gtttcttacg tcctatataa gcatatccct cgtcttttga cctttttata gcattaagta
     5101 tggtaaggac atcttgaagc ggtatctcca tctctaaatc tttaagagtt ttttcaagta
     5161 tttgttgatt ttgagtttga atatcttctt gctttttccc gcctgctaaa tccaaaggta
     5221 aatctttaag ttcaattttg cttcccgcca gattgtaaag atattcaatt acattttcaa
     5281 gttcacgaac attacccggc cagtcatatt ccaaaaattt cgccataacc tcattgctta
     5341 ttaccatctc acttccattt ggaaactttt taagaaacat ttttatcagc aagggaatat
     5401 cgctttttct ctctcttaaa ggaggaatat ttattggaag aacattaagc cgataatata
     5461 agtcttctct gaaatcacct ttttgtacaa gtttatgaag gtctttatta gttgaagcga
     5521 taaccctcac atctattgga ataatcttgg ttcctccgac acgaagaacc tctttttcct
     5581 gaagtactct aagcaatcgt gcttgtattc tgggtgaggc atcaccgatt tcatccagaa
     5641 atatcgttcc tttatgtgcc tgttcaaaaa gtcctgcatg gcctcccttt cttgcaccgg
     5701 taaaggctcc ttcttcataa ccgaaaagtt cactttcaag caagctttcg gtaagtgcag
     5761 caaagttagc tgcaacaaaa ggaccatact gctgtttaga agcattatga attgaatggg
     5821 caaataattc cttgcctgta ccgctttctc cggtcaagag gagggttgaa ttgcttcttg
     5881 aaagcttttt agcaatttct attgtctcgc tcatattttt actcttgccc acaatatcgc
     5941 taaaacggta ttttgcagta tgtccttttt tgaagaggtt gactctgact atattttcca
     6001 tctccataat ttcagacaag tccttaaaag tgattattat accattatta gcattatctt
     6061 caataatcgg catcattgtg agaagaactt ttttaccttt tatctcatga atttcacggt
     6121 acacatcaaa agtcttacct tgaaatgact taatatcaaa gttatttata tcgtttagat
     6181 taatttcggt attgttgaag agtttgtttg cattattatt gctgaacatt atttcaccac
     6241 taccgtcaat acctactata ccgtcattta ttacatttaa tatcgtgttt aactgctccg
     6301 taatatgttt attatcactg agtgtttcta cgattttttt gctggaatac gcaaagtcct
     6361 gcaaatatct tgaagacaat agatttgctc tttcatccat aattccgagc ttaatcatta
     6421 tttctgtgat tgtggtaagg tctattttgc tataaccgat atcaattata tttttgacat
     6481 aatcgggcac taagtgagtt tcaccggttg ttatagaata gtcggcatac tccggaaatt
     6541 cttgacccgg ataataggga tacatcgata tatggttaat acccagttct acaagcagag
     6601 atatggagct ttccgccaaa tatttgttga cgtcaacaaa aagcactttt tccccttcag
     6661 gtatttgtaa aatatcctct aacttagaat gattaagtga tcttctggct accaatacat
     6721 ttgcaccggg agtaatgtgc tttaacgctt cttccttcag atacttatga catatcagaa
     6781 ccagggaatc gcaaatttct tcattgattc cttcttgtat ggaatatcct ttgatgctga
     6841 tttcatctcc aagaagatta gttaattggg caattacatt ctccatgtgt tttttcgaat
     6901 aagttataat agataatcct ttgtttaaag aagatgacac catgaacctc ctcatcaatg
     6961 agtcattaaa taaattatat cataactgta tatcttcttc aacatttaag tcgtcattgc
     7021 atgagtatca gataaacagg tttacattgg attcttcggc atcacccaaa taagtgccta
     7081 ctcctgcaaa ttcaacacca tctattaatt cttcttttgt aatgcccatt acatccattg
     7141 acatggtgca ggcaatcatt tttacaccgt tagccattgc ctttttaatc aaatcttcca
     7201 atgaatcgat atttttatcc ttcattactt ttcgcatcat agctgttccc aatccgccca
     7261 tgttcatttt tgaaagtttt agtttgttaa ctcctcgagg catcattttg ccgaacatgg
     7321 tatcaatgaa aggtttttta atgttttgct tctcggtttt tcttaaaaca ttaagtcccc
     7381 agaatgtgaa aaacatagta accggtcttc ccattgctgc agcaccgttt gcaattacaa
     7441 aggatgccag aactttatcc atgtcgccgc tgaatacaat tatggtttta ccgtcattag
     7501 acggttgaag tcctgttttt tctaaagctg tttcagcttt tttattgtct attccttttt
     7561 gcaaatatac aatattattt ttaccatcct tttctatttt tagaagggta ttgcctgttc
     7621 ttctgctcca tgcttcaaca tctttagcaa atcctgaatc tgttgcagat acttgaacaa
     7681 tatctccatc atttgcattt ttcattgttt catatacctt cataatgggg cccgggcact
     7741 gtaatccgct gcaatccact tgaatagatg atttaatttt tactgttgta tctgttttct
     7801 tttcatttgt attgcttact actaatgtgt catcattact ttctcttccg gtttcgttga
     7861 aatagttgta ataaacagat ttataaaagt ttgttcctgc aggataaact aaaacatttt
     7921 taaatccatt ttccattaaa accctggctg cattgtatga gcgaacccct atagagcaaa
     7981 gtataattat gttatcatct ttatttaatt cgttaattct gccacgaagc tggcccaaag
     8041 gaatatttac ggttccgggt atatcaaata ccatgtgctc cgcttcctct cgtatgtcaa
     8101 gcaatgtgta ttcattttgt tttgaagggt caatatcatt ccacttgctg aatttaacct
     8161 tgttttgaat aatattctca gccacaaatc caagcatatt gacagggtcc ttagctgagg
     8221 aatatggagg tgcgtaagca agttcaagct cttttaaatc ataaatactg cccttcagac
     8281 caattgttgt cgcaattgta tcaattcgtt tatcaactcc gtcctctcct ataatttggg
     8341 caccgaatat tttttgtccg tctgttgaaa atattannnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnncctt tgtttataaa ttcttcaacc tcaataacat caccgattgc
     8521 atatatactt ggatcggatg ttttcagata tttatctaca attattccgc ctctttgatt
     8581 aacttcaagg tctgcttctt ttgccagttg accgtttggg cggacaccga tggacaatat
     8641 aaccatgtct gctgttaagg ttctgccgct tgccaatgtt atggttatta cgccgtcatt
     8701 gctttcgaat ttagatacac catctcctaa aatcaactca atattatttg ataccatgtt
     8761 ttcatgtact aattgtgcca tttcataatc tataggagcc ataacctggt tcattgcttc
     8821 ggcaacagtt acatttaatc ctgcagccgc aagattttct gcaacctcaa gccctatgaa
     8881 accaccccct attacaagag cgtttttagg tttcttttca tttatgaatt ttattatatt
     8941 atctgtatcg ggcacagtcc acaaggtaaa aatacccggt gaattaattc cttcaatcgg
     9001 cggttttaag ggcgatgagc ccgtggatat aatcaaggta tcatagcttt cagtataaat
     9061 ttcgccggtt tttagattct taacatctag ggttttgctc tctctgttaa ttgaaataac
     9121 ttcagactct acacgcacgt caatattata tttttttgac atagcttcgg gagtctgcaa
     9181 caacaaggat gacctttctt ttataacttt tcctatatgg taaggcaaac cgcaatttgc
     9241 atacgatata taaggccctt tttcaaacat tataattttt gatgtttcat caagtcttct
     9301 aagtctggct gctgcggatg ctcctcctgc aacaccgcct attattaaaa ttttcttgct
     9361 cataaatcct cccttatgca tctaaaattt ggcttactat tttttttact tcttcattta
     9421 taattttata ataaatttcc gttccgtttc tttccgctga aagtatgcct gctgacttaa
     9481 gtttggaaag atgctgtgat acagtaggct gcggaacatc caaacagcat tgaatatcgg
     9541 taacattgct ttcctcttct tttataagca ttgccaatat gcaaagtctt acgggatgtg
     9601 ctattgattt aaacagctct gctttttccg acaatacctt tttaactctt tccttattgc
     9661 tgaaatctat cattttaccc tccatatatt gcaatattaa tatattagaa tattagcata
     9721 taataatata atacgcaaga aaaactttat tgtttttaaa aaaatggtta attttatgaa
     9781 ataatggtaa aatgtaaata agaacattaa taatgaggag tactatggaa attataacat
     9841 cttatgaaaa tttacaagag cttattgaaa agaacgatat ggtaatgatt tattttggaa
     9901 gcaagacatg ctcggtatgt aaatccatga cacctaaagt ggagaatatg attaaaagat
     9961 atcctaatat ttatgcaata atggttgaag cggataagag tttagatata tcttccaaat
    10021 ttaatgtgtt taccgtgccg gtaatactat tatatattga aggtaaagaa acaatcaggg
    10081 aagtaagaat aataagcttg gataatttag aacaaaaaat tgcaaggtat tgccagatat
    10141 tttatgttca ttaatttcaa tattcagtat ggttaatcat atcatttgag aaatgaaagc
    10201 ttagatatag tgaagtcaat tattcggtaa tttctccggt tagcatatgc tcagatttca
    10261 tcaattcatc gatactttgt cctctcagcc attcattcat ttcttcaatg ctaaaaatga
    10321 gtggcattct gctatgtatg tgggaaatcg aattattgga atttcgtgtt ataatggtat
    10381 agtaaatatc aatgttttca tcaaacaagc tcaattgctt gtttttattt gcagctatat
    10441 tgtataatcc ggccatataa atattggagc cgggtttatt tattttatat ttatgcttgc
    10501 ctttttcttc ctgcttccat tcataataaa aaga
//
LOCUS       DAOPUE010000062        10456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6417_length_10456_cov_10.746467, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000062 DAOPUE010000000
VERSION     DAOPUE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_10456_cov_10.746467"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKH42_06595"
     CDS             <1..303
                     /locus_tag="PKH42_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20137.1"
                     /translation="DSILEGTINTLLVLRIGLLTQLYSRSITRTEPKRLSKTATVQAC
                     KMLGEIISKDLISIIGIWSKSAFKAVTKVPRNTKDTFMDFFKIKSSDEEAAITDID"
     gene            467..1891
                     /locus_tag="PKH42_06600"
     CDS             467..1891
                     /locus_tag="PKH42_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOA20138.1"
                     /translation="MTNNDKQRNILFFDLNERNLAVMVHSLFSSWMLSFLFEGQIFQQ
                     LAKEYNFASESIIIWSSAAMFSGLLLGGFTVKSKLHAKRLFLFSYPFFIALSLIFFFS
                     PSILWTMGIITGSIVSGCCIAAWGFYFKSVSEKNERIKTAADMLIISNILMILLNVVA
                     INISPNVGLTLSLVMLIFAFILGLRLPTDYNEDYGNKKDGSLSISNLLLFFYIFIVIL
                     TINSGLMYHVVNPAFEHHATLTSWYWAVPYVAVILILRIFSQKIMSSYILYVAIAMIG
                     LSFIAFIALDRSAISYFIINTLMLGSYGVYDLFFWSIFGEMLDYHKNPAKILGTGLSA
                     NVLGIVAGGLIGNSLMANNLYSINPSLFAFAVVCVSLVLLPLLNKYLSTLLKGYTFLT
                     AFVVMPEFKQNSQIDKISEYGNLSEREKQVALLLLQGKTYKTIAGELFISENTVKYYV
                     KNIYSKFNIQSRAELIDMVAEKQN"
     gene            2029..2568
                     /locus_tag="PKH42_06605"
     CDS             2029..2568
                     /locus_tag="PKH42_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20139.1"
                     /translation="MNTIKRKIIIYILIIFFLTAALLQLFSLQSEKLVLHIMVLIIFI
                     IAADGIFIYLLIDKINNLKIARLITENFILKVKTAVISEISVKEAPNKLKCLEDTEIF
                     VSYFGILFNGKIAKFNQDGIRLKEMAIGSDFISFTYGNEKQMQNLRIVRPDIEPEALN
                     EMINKFRFETGITPTIIEG"
     gene            2595..3311
                     /locus_tag="PKH42_06610"
     CDS             2595..3311
                     /locus_tag="PKH42_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20140.1"
                     /translation="MAKKIIGYGNFFCWNCGNRIEKRKKTCPNCGSIYSGDGKYGNVQ
                     ALGAGGIGWSNNVNHYSLKKYFKNDRKYSFIWLIGISIIVPAIMLLSGEIDFDSEGIM
                     VIGGILAVFWGTGLLFIFKKGANEPDWDGIVKDKKVFQKTRRKKDSEGKAYTEEYKEF
                     IVYIRKQNNDIFELKDEDSARYDYFNIGDYLHYHGVKYLNYFEKYDKSLDTIIFCASC
                     RNICDIRDNYCERCGCILLN"
     gene            3325..3873
                     /locus_tag="PKH42_06615"
     CDS             3325..3873
                     /locus_tag="PKH42_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOA20141.1"
                     /translation="MFCENCGTQLPDDSVFCSECGSKIVSVGNESGIDIDSINTNNES
                     GYREQRGPVPPVMRQMATHQPSEPYRKESIPRQPPEPYRRKSTPQQPSEPYRKEAYPQ
                     EPSESYIGASADIIGVGRYILMIFFAWIPIVNFILLIRWSFNKQKPNKSNFAKAMLVY
                     NILALIVYVLYIRLWLSVTSGI"
     gene            3902..4456
                     /locus_tag="PKH42_06620"
     CDS             3902..4456
                     /locus_tag="PKH42_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOA20142.1"
                     /translation="MEILIGLVVIIVLLAISAIVSYNGFVKLRNQIEEAYSTMDVYLK
                     KRYEMIPNLVEVVKQYSIHEKETLERIVQARNMAINARSVEERGQNENILSGAIKSLF
                     ALSEGYPELKANENYLDLQNNLRTLEDEISQSRKYYNGVVKVMNNKVEIFPSNLFAKI
                     FGFNRYPYFMAEEYERQNVEIRFN"
     gene            4468..6342
                     /locus_tag="PKH42_06625"
     CDS             4468..6342
                     /locus_tag="PKH42_06625"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOA20143.1"
                     /translation="MKRNLMLCIFACILMLTIVTETAYGAEAFSITSYDIDITVYENN
                     SYAVIETINVRFIEQRHGIIRSLPLRTNRGKPALISHIDVFEHKFSTEKVSNNIEIKI
                     GDPDKYASFSERYTIGYVYTIGDDYLNDRDELYWNLIGNDWDCTIENVTFKINMPKSF
                     APDRLYFTHGQKGSTKHDGVDWYIDGNTIKGSLMISLKPNDSLTIAMPLPEGYFSQAE
                     RIKINDISYGWVLSLVCAALGTVLWVFMGRKKQIFPTVEFYPPQGLTSADVGYLIDGR
                     VDLFDITSLIIYWADKGYLSITEESVKNMFGTKKIFTLTKLRDIPEESQEYEKEMFEG
                     MFYMGDGINVRTDQLENRFYSVGERIKKQVKSSFEYNEETRIFKKTNTLCRILLLLSG
                     VICTLPFSYRVMKESGNVGEAGLMLKSIIMSAVIMAAFYFFMHILKNWKGMKKGERFK
                     QLFFALFISGAVCGTFIYSLSLSGNAIRGLISLAAAAILGILSNLCSRRTESGRWYEE
                     KLTGFKEFIKAAELDRIQLLADENPNYFYDILPFAMVLGVTDKWAKNFDSIMKEPPNW
                     YNSQGMQGRFSAVSFATGLDNDLKIFSLDIASKHQRSNSSISGGSSGGGSGGGGGRSW
                     "
     gene            6344..>10456
                     /locus_tag="PKH42_06630"
     CDS             6344..>10456
                     /locus_tag="PKH42_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20144.1"
                     /translation="MTVAIKKKINIPDPTMGPIGFTAASFKAAISGIGTILKKPKILF
                     TTLFISALQAALSYLKILMPSSLLVTTASFFTFAQGGMYAGLIGAAGGIIGKGIYAWF
                     MNTLFFSGSRDKSKDDKNENKKTKGKFALFLAGIGIALMVYNFLTGNASIENSVIGLT
                     SLAACLRALKRKNGFLIGFICSFTKGRMSRKAAAVVIKGMTLGFLAGVLSSLKFTGAI
                     CYLVGAVIFIIALILLAGRKRTLAAAAALLIILSNIVPAYSSIANFSDEWQDPYEAYG
                     IQDRTLQGYIALTDAMNASPPMSMDLTLIAVGVRNFDESGRIENRQVLALDGESETFG
                     IDSPEFSEFKFTSAVNLFLEDDGWYADSITQYSVNNAWTNYEREEGLYGNYQKCYFSF
                     KHLGAAGYLFEEYLEDGKVYITIPISFAYGNVMDQSQADSASEIFARVDRVKRASSET
                     GSSKEYSGVWELKEIKTYFVERNRETYRISETHEFKNEFSGNDPTYDTLATVSGNTLN
                     YKIITDYPSVKEVTSLTAEFSELPERMAAFETLRVTVNSSAYYEDQIKNVRNTLDSVP
                     VSVGFLPGLRPEHFNTTESDTGTIVYPLGVNLGEIWFENINGEFQAPAPQGQEEGELL
                     TVELILGPNSPYDATASRHMGNPVIAYIYEWNMTGVIPAGTEGEEDEDEAAASDDGWW
                     TGTDTDEEWSEHADEGETAAIGAMSALAAIAGAGAAAAGSMGIGPGGGYGPYIRRNKD
                     GDLVTKDPVTGEESIYVNNKKTGLYTNPLTGAEYTEDELTSHVASRAENAGVLGQDYE
                     TAQDAIKQQRKDNQKLSQAESEVNFEKSMKKLQKDLETKGAIGDDYADELAKKLKILR
                     SQKKIDGEFNKDDLKKFQKTFNRWTRGEIAGSRGLPKAENDWDIYKQGLALSGEEIAR
                     GDSWKAMGLRVAVGLLTGSLAARGIISLAGAAVVEAGLELGQAGYTMKDYVDKGGDDW
                     TEGAKLAITKTLVGEGIGRSIGLGLKGLGLAASKTGQALSKTKAGKYIVDGISDAVEK
                     TSRVLNANVGDVPKVIKQISKEASESAAAAKKAASMAGKLKTSAANKAAQTADDAAKK
                     AAQSTEDAVKKSAQSTEDAVKKAAPPKNVKDTDAYKQASRQVDDSLNKSKAAAAEKIE
                     KYKNSVNKNPKLAARKAAYEEGGKIGLEKVEKLEQARKALAGNPDSPQLKKEFESAVK
                     DVQINKHAQKIMNEKKLTGNDTRQGFNDFKVKQDAIVSLNTSERLALELGLDPKNVSN
                     VQATNSMDTSGVKTIKGVAVKSDFKTPKTTAPRKFTEADIDLNSLEKKSGEKISIDLD
                     QTYRMEIELKDGTVIAKDIPAYKVKSIQNEEVYKVWNDGQLPLNPDGSVNH"
BASE COUNT         3573 a         1713 c         2263 g         2907 t
ORIGIN      
        1 gattcaatat tggaaggaac aatcaacacc ttgttggtac tgagaatcgg actgttaaca
       61 cagttataca gtaggagcat aacaagaaca gagcctaaga gactgagtaa aactgcaact
      121 gtacaagctt gtaaaatgct tggtgaaata atatcaaagg atttaatatc aataatcggt
      181 atatggtcaa agtccgcttt taaggcagta accaaggttc caagaaatac aaaggatact
      241 ttcatggatt ttttcaaaat aaaaagctct gatgaagaag cggcaattac tgatattgat
      301 tagtataaca aagataggtt tgcaactcaa atccccctac cttttttaag tagggggatt
      361 gtcaattatt aaataatcta ttataatata ttttatacat gcgaaattca attgaaatat
      421 ttgtttgtat gataagtatc tgtgaatgtt caggggggca gaaaagatga caaataatga
      481 caaacaacga aatattctct tctttgattt gaatgaacgc aatttggctg taatggttca
      541 ttccttgttc tcttcatgga tgctgtcatt tctttttgaa gggcaaattt ttcaacaact
      601 tgccaaagag tataatttcg catcggagtc aataattatt tggagttctg ccgctatgtt
      661 ttcgggatta ctgcttggcg gatttactgt aaaaagtaaa cttcatgcaa aacggctttt
      721 cctcttttct tatccctttt ttatcgcctt gtccttaata tttttctttt cgccttcaat
      781 actatggact atgggcataa taaccggttc cattgtttcg ggatgctgta ttgctgcatg
      841 gggattttat tttaaaagtg tatcagaaaa gaatgaacga ataaaaacag cagcagacat
      901 gctgattatt tccaatatat tgatgatatt attaaatgta gttgcaataa atatatctcc
      961 caatgtaggg cttaccttgt ctcttgttat gttaatattt gcatttatac tgggcttaag
     1021 actacccacg gattacaatg aggactacgg taataaaaag gatggcagct taagcatttc
     1081 aaatctttta ctcttctttt atatctttat agtaatattg acaataaatt ccggattaat
     1141 gtaccatgtg gtaaatcccg cctttgaaca ccatgcaaca ctcactagct ggtattgggc
     1201 agttccctat gttgccgtaa tattgattct tagaattttt tctcaaaaga taatgtcatc
     1261 atatatacta tatgtagcaa ttgcaatgat tggtctttca tttattgctt ttatcgccct
     1321 tgaccgctct gcaataagtt attttataat aaatacactt atgcttggtt cctatggtgt
     1381 ttatgatttg tttttttgga gcatatttgg ggagatgctt gattatcata aaaaccctgc
     1441 caaaattctt ggcacaggtc tttctgccaa tgtattgggt atagtagccg gcggacttat
     1501 aggcaatagc ttaatggcaa ataatttata cagcataaat ccatcactgt ttgcctttgc
     1561 ggtagtttgt gtatctttgg tactattgcc tcttctgaac aaatacctgt caactttgct
     1621 taaaggatat acatttctta ctgcatttgt cgtaatgccg gaatttaaac aaaacagtca
     1681 aatagacaaa atatcagaat acggcaattt gtcggaaaga gaaaaacagg tggcattact
     1741 tcttctacag ggaaaaacct acaaaactat tgcgggtgag ctttttataa gcgaaaatac
     1801 cgtaaaatat tacgtaaaaa atatttattc caaatttaat attcaaagcc gggcagaact
     1861 cattgacatg gtggcagaaa aacaaaatta aacacctacc cgaaaaaggt agggttgttt
     1921 tttaaagaat tacccttttc gggtgatttt tttttgcagt ttaactgtta taatctctaa
     1981 tcaagtaaga attctgtaac aataacaatg cagtttcggg tggttgggat gaatacaata
     2041 aaaagaaaaa tcattattta cattttaata atattttttt tgacggcagc tttactccag
     2101 cttttttctc ttcaaagtga aaaacttgtt cttcatatca tggtattgat aatatttata
     2161 attgctgctg atggtatatt tatatacctc ttgatagata aaattaataa tttgaaaatt
     2221 gcaagactga taaccgaaaa cttcatattg aaggtcaaga ctgcagttat tagcgaaatt
     2281 tcagtcaaag aagcgccaaa taagcttaaa tgcttagaag atacagaaat atttgtatca
     2341 tattttggaa ttctctttaa cggtaaaatt gcaaaattta atcaagatgg catccggctg
     2401 aaagaaatgg ccattgggag tgattttata tcatttacct acggcaatga aaaacaaatg
     2461 caaaatttgc gtattgtacg gccggatatt gagcctgagg ctttgaatga aatgattaat
     2521 aaattccgtt ttgaaacggg gattacaccg acgataatcg agggataaag aaatatattg
     2581 caatgaggta gaatatggct aaaaaaatta ttggttacgg aaactttttt tgttggaatt
     2641 gcggcaatag aattgaaaaa aggaaaaaaa cgtgtccaaa ctgcggctcc atatacagcg
     2701 gagatggcaa gtacgggaat gttcaagcat taggagcggg aggtataggc tggtcaaaca
     2761 atgtaaatca ttacagttta aaaaaatatt ttaaaaatga cagaaagtac agctttattt
     2821 ggctcatagg catcagtata atagttcccg caataatgct tctttcaggg gagatagatt
     2881 ttgacagtga gggtattatg gttatcggcg gtattttggc ggtattctgg ggaacaggtc
     2941 ttttatttat atttaagaaa ggagctaatg agccggactg ggatggaatt gttaaagaca
     3001 aaaaagtatt tcagaaaacg agaagaaaaa aagacagcga agggaaggct tataccgaag
     3061 aatacaaaga atttattgtt tatatacgca agcagaacaa tgatattttt gaattaaagg
     3121 atgaggatag tgctcggtat gattatttca atataggtga ttatctgcac tatcatgggg
     3181 tcaaatattt aaattacttt gaaaagtatg ataagtcctt ggatacaata attttttgcg
     3241 caagctgtcg caatatttgt gacatacgag ataattattg tgaaaggtgc ggatgtatat
     3301 tattaaatta aaaggaggaa aacaatgttt tgtgaaaatt gcgggacaca gcttcccgat
     3361 gactctgtgt tttgttcgga atgcggcagc aaaatagtat cagttggaaa tgaatccgga
     3421 atagatatag acagtatcaa tacaaataat gagtccggct accgggagca gcgaggaccg
     3481 gtgcctcctg ttatgagaca aatggcgacg caccagccgt cagagcccta caggaaagaa
     3541 tcgataccgc gtcagccgcc agagccctac aggagaaaat caacaccaca acagccatct
     3601 gaaccataca gaaaagaagc atacccacaa gagccgtctg aatcctacat aggagcaagt
     3661 gcggatatta taggagtcgg ccggtatata cttatgattt tttttgcctg gattcctata
     3721 gtcaatttta tactgcttat aagatggagt tttaataaac aaaaacccaa taaaagcaat
     3781 tttgcaaagg caatgctcgt ttataatatt ttggcattaa tagtttatgt tttgtatatt
     3841 cggctatggc tgagcgtgac ttcaggtatt taataatatc tataaaagaa aggggaagaa
     3901 gatggaaata ctaataggat tggttgtcat catagtttta ttggcaatat cagcgattgt
     3961 gtcatataac ggttttgtaa agcttcgaaa tcaaattgaa gaagcttatt ccaccatgga
     4021 tgtttattta aagaaacgat acgaaatgat tccgaattta gtggaagtag taaaacagta
     4081 ttcaatacat gaaaaagaaa ccttagaaag aatcgtccag gcaaggaaca tggcaataaa
     4141 tgcaaggtct gtagaagagc gggggcagaa tgaaaacatc ctgtccggtg ctataaaatc
     4201 tctctttgct ctttctgaag gctatcctga attgaaagca aacgaaaatt atctggactt
     4261 gcaaaacaat ctgagaacct tggaagatga aatatctcag tcccgtaaat actataacgg
     4321 cgtagttaaa gttatgaata acaaggttga aatattccct tcaaacttgt ttgctaaaat
     4381 ttttggattc aacagatatc catattttat ggctgaagaa tatgagcgtc aaaatgtaga
     4441 aattagattt aattagaggt agggtcaatg aaaagaaacc ttatgctttg tatttttgct
     4501 tgcatcctca tgttaactat tgtgacggaa acagcttatg gtgctgaagc attcagtatt
     4561 acttcatacg atatagatat aacggtttat gaaaataaca gctatgcggt aatcgaaaca
     4621 attaatgtca ggtttatcga gcaaaggcac ggcatcataa gaagtctgcc tttgagaaca
     4681 aacagaggaa agcctgcact aatttctcat atagatgttt ttgagcacaa gttttctact
     4741 gagaaagtct caaataatat tgaaattaaa ataggcgacc ctgacaaata cgcttccttc
     4801 tcggaaagat atacaattgg ttatgtgtat accataggag atgattattt aaatgacaga
     4861 gacgaacttt actggaactt gataggaaat gactgggact gtacaattga aaacgttaca
     4921 ttcaaaataa acatgccaaa gtcttttgcg cctgacaggc tttatttcac ccatggtcaa
     4981 aagggaagca caaagcatga cggtgtggac tggtatattg acggaaatac cataaagggc
     5041 tccttgatga tttctcttaa gcctaatgat tcacttacaa ttgccatgcc tcttccggaa
     5101 ggatattttt cacaagcaga aagaataaaa attaatgata tatcttacgg atgggtgtta
     5161 tctcttgtct gtgcggcttt aggcacagtc ttgtgggtat tcatggggcg caaaaaacaa
     5221 atatttccaa cagttgaatt ttacccccct caagggttga ccagcgcaga tgttggttac
     5281 ttaatcgacg gcagggtaga tttgttcgat ataacatctc taataattta ttgggcagat
     5341 aaaggatatt tgtcaatcac cgaagaaagc gtaaaaaata tgtttggcac aaaaaagatc
     5401 tttacactta caaaacttag agatattccg gaagaaagcc aggaatatga aaaggaaatg
     5461 tttgagggaa tgttttacat gggggacggg ataaatgtaa gaacagatca attggaaaac
     5521 aggttttact ctgtggggga gagaataaaa aagcaagtta aatcaagttt tgaatataac
     5581 gaagaaaccc gaatcttcaa aaaaacaaat accttgtgcc gcattctgct tctcttgtca
     5641 ggagtaatat gcacactgcc cttttcctat agggttatga aagaatcagg caatgttggt
     5701 gaagcaggac ttatgttaaa gagtataata atgtctgcag ttataatggc agctttttat
     5761 ttctttatgc atattttgaa gaattggaag ggaatgaaaa aaggggaaag gtttaaacaa
     5821 ttattttttg ctctgtttat tagcggagca gtgtgcggta cttttatata ttcattaagc
     5881 ttatccggca atgcaataag gggtttaatt tcattagcag cagcagcgat acttggaatt
     5941 ttgtcgaacc tttgcagccg caggacagaa tcaggcaggt ggtacgaaga aaaattaaca
     6001 ggatttaagg agtttattaa ggctgcggaa ttagacagaa ttcagcttct ggctgatgaa
     6061 aatcctaact atttttatga tatactaccc tttgcaatgg tacttggcgt aacggacaaa
     6121 tgggcgaaaa actttgactc tatcatgaaa gagccgccca attggtataa ttcgcaaggc
     6181 atgcagggca ggttcagcgc ggttagtttt gcaacgggat tagataatga tttgaaaata
     6241 ttttcattag atatagcctc aaaacaccaa aggagcaact caagcatcag cgggggaagc
     6301 tctggaggcg gttcaggagg cggcggagga aggagctggt aagatgactg tagcaataaa
     6361 aaagaaaatt aatattcccg acccgaccat gggacctatc gggtttaccg ctgcaagctt
     6421 taaggcagca atttctggaa tcggcaccat actaaaaaaa ccgaaaattc tattcacaac
     6481 tttattcata tcggcgctac aggcagcctt atcctacctg aaaattctca tgccctcttc
     6541 tttattggtt acaacagcaa gtttttttac ttttgcccaa ggaggaatgt atgcagggct
     6601 tattggagct gcaggcggaa ttatcggcaa aggaatatat gcatggttta tgaatacact
     6661 gtttttttca ggctccaggg ataaaagcaa agatgataaa aatgaaaata aaaaaacaaa
     6721 gggtaagttt gctctattcc tggcaggaat cggtattgca cttatggtat acaatttttt
     6781 aaccggcaat gcatcaatag aaaacagtgt tatcggctta acgtctcttg cagcctgcct
     6841 aagagcactt aagagaaaga acggtttttt aataggtttc atatgctcct ttacaaaggg
     6901 gcggatgtca aggaaggcgg cagctgtcgt tataaaggga atgaccttag ggtttttggc
     6961 aggtgttttg tcatccctta aattcacggg agcaatctgt tatctggttg gagctgtaat
     7021 atttataatt gcccttattc tcttggccgg caggaaaaga actcttgcgg cagctgcagc
     7081 cctgcttata attttaagca atatagtgcc cgcctattca tcaattgcaa atttttccga
     7141 tgaatggcag gacccttacg aggcatatgg aattcaagac agaaccttac agggttatat
     7201 agcattaacc gatgcaatga atgcttcccc tccaatgtcc atggatttaa ctttaatagc
     7261 cgtgggagta agaaactttg atgagtcggg aagaatagaa aaccggcagg tattagccct
     7321 ggatggtgaa agtgaaacct tcggcattga tagtcccgag ttttccgaat ttaaattcac
     7381 aagcgcagta aatctcttcc ttgaagatga cggatggtac gctgattcca taacacaata
     7441 ttcggtaaat aacgcctgga caaattatga aagagaagaa ggcttgtacg gcaattacca
     7501 gaaatgttat ttttcattta aacatttggg tgcagccggc tatttattcg aagagtattt
     7561 ggaagatggc aaggtttata ttacaatacc catttcattc gcatacggta atgtcatgga
     7621 ccaatcccag gcagacagcg catccgaaat atttgcacgg gttgaccgcg taaaaagagc
     7681 aagcagtgaa accggcagtt caaaggagta ttcaggagtt tgggagctta aagaaataaa
     7741 gacatatttt gtagaacgta accgggagac ataccgaata tcagaaacgc atgaattcaa
     7801 aaatgaattt tcaggcaacg acccaactta tgacaccctt gccactgttt ccggaaacac
     7861 cttaaattac aaaatcataa ctgattatcc ttccgttaaa gaggttacga gcttaacggc
     7921 tgaatttagt gaactgcctg aaaggatggc agcttttgaa acattgagag taacggtaaa
     7981 ttcgtcagct tattatgaag accaaataaa aaatgtaaga aatacactag actccgttcc
     8041 ggtttctgtg ggattcttgc ccggccttcg cccggaacac tttaatacta cggaatctga
     8101 tacaggtaca atagtatacc ccctaggagt taatttggga gaaatttggt ttgaaaatat
     8161 aaatggagaa tttcaagccc cagctcctca gggacaggaa gaaggcgagc ttttaactgt
     8221 cgagctgatc ttaggcccta acagccctta tgatgcaact gcttcaagac atatgggaaa
     8281 tcctgtaata gcctatattt atgaatggaa tatgacgggg gtaattcctg caggaaccga
     8341 aggcgaagaa gatgaagatg aagctgccgc cagtgatgat ggctggtgga caggtactga
     8401 tacagatgaa gaatggagcg aacatgcaga cgaaggagag acagcagcta tcggagccat
     8461 gtcagctctt gctgctatag cgggtgccgg agcggcagca gcgggaagca tgggcattgg
     8521 tccaggtgga ggttacggtc catatataag aaggaacaag gatggggacc ttgtaaccaa
     8581 ggaccccgtt acaggggaag aaagtatata tgttaacaac aaaaaaacag gtctctatac
     8641 aaatccatta acaggagctg aatacacaga agatgagcta acaagtcatg tagcttcacg
     8701 ggcagaaaat gcaggtgtat taggccagga ttatgaaact gcacaagatg caataaaaca
     8761 gcagcgtaag gataatcaga aattaagcca ggcagaaagt gaagtaaatt ttgaaaaaag
     8821 catgaaaaaa cttcaaaaag atttggaaac caaaggagcc atcggagacg attatgcaga
     8881 tgagttagcg aagaaactta aaatacttcg aagtcagaaa aaaattgacg gtgagtttaa
     8941 taaagatgat ttaaaaaaat tccagaaaac atttaacaga tggacaagag gagaaatagc
     9001 aggaagcaga ggactgccaa aagcagaaaa tgattgggat atatacaaac aaggattggc
     9061 attatcagga gaagagatag caaggggaga cagctggaag gccatggggc ttcgcgtggc
     9121 tgtggggctt ttaacaggca gtcttgcagc ccggggtata atttcccttg ccggtgctgc
     9181 tgttgtggaa gccggtttgg agcttggtca ggcaggttat acaatgaagg attatgttga
     9241 caagggtgga gatgattgga cagaaggagc gaagcttgca atcacaaaga ctttggtggg
     9301 cgaaggtata ggaaggtcta taggtttggg attaaaaggc cttggtcttg cagcgtcaaa
     9361 gacagggcaa gcattatcta aaacaaaagc aggaaagtat attgttgatg gaatttcaga
     9421 tgcagtagaa aaaacttcaa gagtactaaa tgccaatgta ggagatgtgc caaaggtaat
     9481 aaagcaaatc agcaaggaag caagtgaatc agctgctgca gctaaaaaag cagcctcaat
     9541 ggcaggaaaa ttaaaaacat cagctgctaa caaagcagct caaacagctg atgacgcagc
     9601 caaaaaagca gcccaaagca ccgaggatgc tgtcaaaaaa tctgctcaaa gcaccgagga
     9661 cgcagtcaaa aaagctgcac caccgaaaaa tgtgaaggat acagatgcat acaagcaggc
     9721 atccaggcag gttgacgata gtttaaataa atccaaagct gctgcagctg agaaaataga
     9781 gaaatacaag aatagtgtaa ataaaaatcc taaattagca gccaggaaag cagcttatga
     9841 agaaggcggt aaaattggat tggaaaaggt tgaaaagctt gagcaggcaa ggaaggcatt
     9901 agctggcaac cctgattctc cacagcttaa aaaagaattt gaaagtgcag ttaaagatgt
     9961 tcaaataaac aaacatgcac aaaaaataat gaacgaaaaa aaattgacag gcaatgatac
    10021 acgccaggga ttcaatgact ttaaggtaaa acaggatgca atagtatcac ttaatacaag
    10081 tgaacgttta gcattggaat taggtcttga ccccaaaaat gtaagcaatg tccaggctac
    10141 aaattccatg gatacatcgg gagttaaaac tataaagggt gttgcagtta aaagtgattt
    10201 taaaactcca aaaaccacag caccaaggaa atttacggaa gctgatatag atttaaactc
    10261 tcttgaaaag aaaagtggag aaaaaataag catagatttg gaccagacct atagaatgga
    10321 aattgagctt aaagatggca ctgtaattgc caaagatatt cctgcttata aggttaaaag
    10381 cattcaaaac gaagaagtat acaaggtatg gaatgacgga cagcttcctt tgaaccctga
    10441 cggttcggta aatcat
//
LOCUS       DAOPUE010000063        10395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6470_length_10395_cov_9.969052, whole genome shotgun sequence.
ACCESSION   DAOPUE010000063 DAOPUE010000000
VERSION     DAOPUE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_10395_cov_9.969052"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..486
                     /gene="rplK"
                     /locus_tag="PKH42_06635"
     CDS             61..486
                     /gene="rplK"
                     /locus_tag="PKH42_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOA20145.1"
                     /translation="MAKKVTAVVKLQIPAGKATPAPPVGTALGPHGVNIMGFCKEFNA
                     KTADQAGMIIPVVLTVYQDRSFTFITKTPPVAVLIKKAVGIESGSGEPNKKKVATLSK
                     AKCEEIARLKMPDLNANTIEAAMSLVAGTARSMGVTVEK"
     gene            542..1240
                     /gene="rplA"
                     /locus_tag="PKH42_06640"
     CDS             542..1240
                     /gene="rplA"
                     /locus_tag="PKH42_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOA20146.1"
                     /translation="MPKRGKKYQDSIKSIDRLKLYDMQEAVEAMLSTAKANFDETVEV
                     HLRLGVDSRHADQQVRGAVVLPHGTGKTVRVLVFAKGDKAKEAQEAGADFVGEAELAD
                     KIQKENWFDFDVVIATPDMMGVVGKLGRVLGPKGLMPNPKSGTVTFDVGKAIKEIKAG
                     KVEYRLDKTNIIHVPVGKKSFGAEKLIDNLKVILEAVIKAKPAAAKGKYLKSVTIAST
                     MGPGIKLNTTKLMD"
     misc_feature    1268..1384
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1397..1900
                     /gene="rplJ"
                     /locus_tag="PKH42_06645"
     CDS             1397..1900
                     /gene="rplJ"
                     /locus_tag="PKH42_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOA20147.1"
                     /translation="MNQTVLEQKKQVVQEIAEKIRNAKSIVLVEYNGLTVDMANELRN
                     KCREAGVEYKVYKNTLMRFAFEELGYDGITPNLEGPNAIAFSYEDEVSAAKVTNDFAK
                     TSNDTIVIKAGIAEGRVMNASEVTALASVPTREILIAQLAGVLQGNIRNLAYMLDQIS
                     KKEGEAA"
     gene            2049..2432
                     /gene="rplL"
                     /locus_tag="PKH42_06650"
     CDS             2049..2432
                     /gene="rplL"
                     /locus_tag="PKH42_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOA20148.1"
                     /translation="MASEKITNFIEEIKKLTVLELNELVKAIEEEFGVSAAAPVAVAG
                     GAQAGAAAPAEEEQTEFDVVLTSAGAGKIKVIKVVRELTGLGLKEAKDAVDGAPTTIK
                     TGVSKEEAEKAKEALEAEGASVEIK"
     gene            2828..6625
                     /gene="rpoB"
                     /locus_tag="PKH42_06655"
     CDS             2828..6625
                     /gene="rpoB"
                     /locus_tag="PKH42_06655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOA20149.1"
                     /translation="MPHPVKIGNRVRMSYSKIKEVLELPDLIEVQKKSYEWFLNEGLK
                     EVFDDISPIEDYTGNLILEFVDHSLYSEPKFSQIECKERDATYSVPLKLKVRLINKET
                     GEIKEQEAFMGEFPLMTEKGTFIINGAERVVVSQLVRSPGVYYNMTRDKVGKELYDAT
                     VIPNRGAWLEFETDSNDIVYVRVDRTRKLPATVLVRAMDVSSNSELIDILGETEQLLK
                     TLEKDNTTNSEEALIEIYKRLRPGEPPTYDSAKSLIKSLFFDFKRYDLAKVGRYKLNK
                     KLSLENRIIGKRAAEDIINTETGEVLVTKDSIFDDESILKLEAAGIFSVKIVNSDDKV
                     TKVLSNKFVDPRVFELDKLGIDLEKIGIKEKVYYPVMKKILEENHSAEDLEKALIESL
                     KQVPAAENNIFEKDKEENYSISPKHIVKDDMYAIVNYHFGLFRGIGKTDDIDHLGNRR
                     LRSVGELLQNQVRVGLSRMERVVRERMTIQDVDVVTPQVLINIRPVSAAIKEFFGSSQ
                     LSQFMDQTNPLAELTHKRRMSALGPGGLSRDRASFEVRDVHHSHYGRMCPIETPEGPN
                     IGLITSLATYARINDYGFLESPYRRVDKKTGKVTDIIDYLTADVEDTKIIAQANEPLN
                     EDGTFVHSRVVVRGIDGINEIVSRDMVDYMEVSPKQVISIGTGMIPFLENDDANRALM
                     GANMQRQAVPLLVTETPIIGTGLEHKAAMDSGAVIIAEHDGKVVSVTASEILVKRTDN
                     GMIDKYTLLKFKRSNSGTCINQRPIVYKGDEVKRGQIIADGPSTHDGDMAIGKNLLIG
                     FMTWEGYNYEDAMLINERLVMDDVLTSVHIEEYESEARDTKLGPEEITRDIPNVGDDS
                     LKDLDERGIIRIGAEVEAGDILVGKVIPKGETELTAEERLLRAIFGEKAREVRDTSLK
                     VPHGESGIVVDVKVFTRENGDELSPGVSKLVRVYIATKRKINVGDKMCGRHGNKGVIS
                     MIMPEEDMPFLPDGTPLEVVLNPLGVPSRMNIGQVLEVHLGLAAKKLGWEVATPVFDG
                     AVEDDIWDALEMAGYPRSGKIDLRDGRSGEYFEGPVTVGYMYMLKLHHLVDDKIHARS
                     TGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQEILTVKSDDVNGRVKTY
                     EAIVKGENIPEPGIPESFKVLVKELQSLALDIKIITSDDEVEIIETIDNDDDDLPIDS
                     ESLGDLALIEGDMEGMQIDSDDIEDIDDIDDLDDLGDIDDIDSDELDELDIEEDFDDE
                     DKF"
     gene            6757..10305
                     /gene="rpoC"
                     /locus_tag="PKH42_06660"
     CDS             6757..10305
                     /gene="rpoC"
                     /locus_tag="PKH42_06660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOA20150.1"
                     /translation="MIDYNNFESIRIGLASPDKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECHCGKYKRVRYKGVVCDRCGVEVTKAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFAQYVVTEAGDTSLSEKQLLTEMEYREYKE
                     HYKNAFKAEMGAEAIKKLLEKIDLESEAAELKAQLRDSKGQKKIRITRRLEVIEAFRA
                     SGNRPEWMILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFNQCGLPKRMALELFKPFVMKKLVETGAAHNIKSAKKKVEK
                     VDTAVWDVLDEIIKDHTVLLNRAPTLHRLGIQSFEPVLVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAESRFLMMSVNNILAPKDGRPITTPTQDMVLGSYYMTLEIED
                     SKGQGMIFKDYDEMKMAYFNKFIDLHAKVKVRRTVGKESKLVESTVGRFIFNEGIPQD
                     LGFVDREKDKFSLEIDKKVTKKILEEIIYRCFRKYGNARTAEVLDGIKQKGFRYSTLG
                     AITISVSDITVPEEKKTLLEQAEKQVLEYEKKYRRGLMTDEERYENVIKIWNQTTEDV
                     TNALMNNLSEINPIYIMSVSGARGGRNQIKQLAGMRGLMASAIGKTVEVPVKSNFREG
                     LSVLEFFLSATGARKGLTDTALRTADSGYLTRRLVDVSQDVIVREADCGTSDGIEVED
                     FIDGKEEIERLSERCADRFAADDVVNTETGEIFVKAGEFISEEMAIKIQNSGIKKVKV
                     RSVLTCKTIHGVCAKCYGTNLATGKLVNVGAAVGIIAAQSIGEPGTQLTMRTFHTGGI
                     AAAADITQGLPRIEELFEARKPKGQAIISEIPGKVTLSERAHKREVTITNYEDKESKT
                     YLIPYGSKLLVNEGDYIEAGDKITEGSINPHDILNIKGMKALESYILMEVQRVYRMQG
                     VDISDKHVEVIIRQMVNKIRIEESGDTSLLPGSLVSNYEFYQQNEAAIKEGKEPAKGE
                     IALLGITKSSLATDSFLASASFQETTRVLTDAATKGKIDNLIGLKENVIIGKLIPAGT
                     GMKRYRNIGITVNDIEKPDDNTEKTDDDEKSIKDAG"
BASE COUNT         3716 a         1550 c         2400 g         2729 t
ORIGIN      
        1 attgagaaag tataattact tttcaatata acatcgttat gcgatgaagg aggtgcaaat
       61 atggcaaaaa aagtaacagc tgttgttaaa ctgcaaatac ctgctggcaa agctactccg
      121 gctccaccgg ttggtacggc attaggacct catggtgtga atattatggg attttgtaaa
      181 gagtttaatg caaaaacagc cgatcaggca ggtatgatta taccggttgt attgactgtt
      241 tatcaggata gatcatttac atttatcaca aaaactccgc cggttgcagt tctaatcaaa
      301 aaggcagttg gtattgaaag cggttcggga gaaccaaaca aaaagaaagt tgcaacactt
      361 tcaaaggcaa aatgcgaaga aatcgcgaga ttaaaaatgc ctgatttaaa tgctaatact
      421 atcgaagcag ctatgagcct ggtggcaggt actgcaagaa gtatgggagt aacagtagaa
      481 aaatagttat ggcaggtggg aggccgatag aaggctgctt gaccacaagg aggtaaataa
      541 aatgccaaaa agaggaaaga agtatcaaga cagtataaaa tcaattgaca gattgaaatt
      601 atacgatatg caagaagctg tagaagcaat gctttcaaca gcaaaagcta attttgatga
      661 aacagttgaa gttcatttaa gattaggtgt agattcaaga catgcagacc aacaagtaag
      721 aggtgctgta gtactgcctc acggaactgg aaaaacagta cgtgttttgg ttttcgctaa
      781 gggcgacaaa gctaaggaag ctcaggaagc cggagcagat tttgtgggcg aagctgaatt
      841 ggctgacaaa attcaaaaag aaaactggtt tgatttcgat gttgttatag caacacctga
      901 tatgatgggt gtagtcggaa aactgggaag ggttttagga cctaaaggtt taatgccaaa
      961 cccaaaatca ggaacagtta cctttgatgt aggtaaggct attaaggaaa ttaaggcagg
     1021 taaagttgaa tacagacttg acaaaacaaa cattattcac gttcctgtag gaaagaaatc
     1081 attcggtgca gaaaaattaa tagataactt aaaggttatt cttgaagcag taattaaagc
     1141 aaaaccggct gctgccaaag gtaaatactt aaagagtgtt acaattgcaa gcacaatggg
     1201 accgggaatc aagcttaaca ctactaaatt aatggattaa tctgaagcga aagcaaacag
     1261 attaaagcaa aaattgaata taataccgca gacagccggt actccatgag agtttaaata
     1321 tccggccgag gtgagataat agattctttc tcttcgtctg cggagagaat tttttgttta
     1381 aaggaggtgc aacaagttga atcaaacagt attggagcaa aagaaacagg tagtacaaga
     1441 aattgctgaa aagataagaa acgctaaatc tatcgttttg gttgagtata atggcttaac
     1501 ggtagatatg gctaatgaat tgagaaacaa gtgcagagaa gcaggtgttg aatataaagt
     1561 ttataaaaat actttaatgc gttttgcatt tgaagaatta ggatatgatg gcattacacc
     1621 taatttggaa ggacctaatg caattgcttt ttcatatgag gatgaagtat ctgcagcaaa
     1681 ggtaaccaat gattttgcaa aaacaagcaa tgatacaata gtaataaagg ccggaatagc
     1741 tgaaggtagg gttatgaatg ccagcgaagt tacagcattg gcaagcgtgc cgacaagaga
     1801 aattcttatc gcacagctgg caggcgtatt acaaggaaat atcagaaatc ttgcttacat
     1861 gcttgaccag ataagcaaaa aagaagggga agcagcttaa acctaaagtt tgtcattctg
     1921 agggcttcag cccgaagaat ctcatatacc aagtctgact gatgagatcc ttcactaacg
     1981 ttcaggatga cgtttaaaaa caaaaataac aaatatatat tattattata ccaggaggaa
     2041 aatataaaat ggctagtgaa aaaatcacaa attttattga agaaataaaa aaattaacag
     2101 tattggaatt aaatgaatta gtaaaagcaa tagaagaaga atttggcgta tcggcagcag
     2161 ctccggtagc tgtagcagga ggagctcaag caggtgcagc agctcccgca gaagaagaac
     2221 agacagagtt tgacgttgtt cttacatcag ccggtgccgg taaaatcaaa gttataaagg
     2281 tagtaagaga attaacaggt cttggattga aggaagcaaa ggatgcagtt gatggagctc
     2341 ctactacaat taagacaggc gtttcaaaag aagaagctga aaaagctaaa gaagcattgg
     2401 aagcagaagg tgcttcagta gaaattaaat aataagtgca gggacactta tagtataccc
     2461 gaaacgagaa agaaacttta cataagtaga gtttctttta ttttaatagc aatgatgagg
     2521 gctatgctct caatatgaaa gttattcata ataattacga cctatgttaa ggtataggaa
     2581 ctcagcttat tataatatgc ataatatacc aaaatttaca aataataaga aggcggtttt
     2641 aaagtttata tggatacaaa caaagaaatc actcggaatt tgtagagaat tgttaaaaaa
     2701 tatttattaa taatgcaaaa aatgtgttga cataaaaatt tattagtgat agtattgtat
     2761 attgccatga gtgtgcaatg ttggcataga atttgtaagt caaaattatt tactaagggg
     2821 tgaaaagatg ccgcatcctg tgaaaatagg taatagggta agaatgtctt actctaaaat
     2881 taaagaagtt ttagaattgc cggacttaat cgaagttcag aaaaaatcgt acgagtggtt
     2941 cttaaatgaa gggctgaaag aggtttttga tgacatttct ccgatagagg attataccgg
     3001 caatttaatt ctggagtttg ttgatcattc tttatacagt gagccgaagt ttagccagat
     3061 tgagtgtaaa gaaagagatg caacatattc tgtacctcta aaacttaaag tgcgattgat
     3121 aaacaaggag acgggagaaa ttaaggagca agaagcgttt atgggtgaat ttcctttgat
     3181 gaccgaaaaa ggaacattta ttataaatgg tgcagaacgt gttgttgtca gtcagttagt
     3241 tcgctcaccc ggtgtttatt acaacatgac gagggataag gttggtaagg aattatatga
     3301 tgcaacagtt attcccaaca gaggagcatg gttggaattt gaaacagact ctaatgatat
     3361 tgtatacgtt agggttgaca gaacaagaaa attacctgcc actgtgcttg tgagagctat
     3421 ggatgtttct tctaattcag agctcattga tatattgggg gaaacagagc aacttttaaa
     3481 aactttggaa aaggacaaca ctaccaactc cgaagaagca ttaatagaaa tatataaaag
     3541 attgagaccc ggcgagccac ctacatatga tagtgcgaag tcattgatta aatctttatt
     3601 ttttgatttc aaaaggtatg atcttgcaaa agtaggcaga tacaaattaa ataagaagct
     3661 tagtttggaa aacagaatta ttggaaagcg tgcagctgaa gacataatta atacggaaac
     3721 aggagaggta ttagttacta aagacagtat atttgacgat gaatcaattt taaaattgga
     3781 agcggcaggt attttttccg ttaaaattgt gaattccgat gataaggtta caaaggtatt
     3841 aagcaataaa tttgttgacc caagggtatt tgaattagat aaattaggta tagatttaga
     3901 aaagatagga ataaaagaga aagtttacta tccggtaatg aaaaagatac tggaagaaaa
     3961 ccactctgca gaggatttag aaaaagcttt aattgaatcc ttaaagcagg ttcctgctgc
     4021 tgaaaacaat atatttgaaa aggataagga agaaaattat tcaatatctc cgaaacatat
     4081 tgttaaggat gatatgtacg caatagtgaa ttatcatttt ggattattta gaggcatagg
     4141 caagactgat gatatcgacc atcttggaaa cagaagactg cgctccgtgg gtgagcttct
     4201 tcaaaaccag gttagagtag gattgtcaag aatggaaaga gtagtcagag aaagaatgac
     4261 tattcaggat gtcgatgttg taactccaca agttcttata aatatcagac ctgtgtctgc
     4321 agcaataaaa gaatttttcg gctccagcca gttgtcacag tttatggacc agacgaatcc
     4381 cttggctgag cttactcata aaagaagaat gtcagctctg ggacccggtg gattaagcag
     4441 agatagagcc agttttgagg taagagacgt tcaccattct cattacggca gaatgtgtcc
     4501 cattgaaaca cctgaaggtc ctaatatagg tcttataact tctcttgcaa catatgcaag
     4561 aataaatgat tacggatttt tagaatctcc ttacagaagg gttgataaaa aaacaggaaa
     4621 ggtaaccgat attattgatt atcttactgc agacgtggaa gatacaaaaa taattgcaca
     4681 ggctaatgaa cctttgaatg aagacggtac atttgtgcac agccgtgttg ttgtaagggg
     4741 aattgacggt ataaatgaaa ttgtatcaag agatatggtt gattatatgg aagtttctcc
     4801 aaaacaggta atttccatag gtacaggtat gataccattt ttggaaaatg atgatgcaaa
     4861 ccgtgcttta atgggtgcga acatgcaaag gcaggcagta cccttgttgg ttaccgaaac
     4921 gcctataatc ggaacaggtt tggagcacaa ggcagctatg gattcaggag ctgttattat
     4981 tgctgagcat gacggaaaag tagtatcggt aactgcctcg gaaattttag taaaaagaac
     5041 cgacaacggt atgattgaca agtatacttt gcttaagttt aagcgctcga acagcggtac
     5101 ttgtataaat caaagaccaa tagtatataa aggcgatgaa gtaaaaagag gacagattat
     5161 tgctgacgga ccttctactc atgatggtga tatggctatc ggaaaaaatc ttttaatagg
     5221 atttatgacg tgggaaggat ataactacga agatgcaatg ctcataaatg aaagacttgt
     5281 tatggatgat gtattaacat ccgtccatat tgaagaatat gaatcagagg caagggacac
     5341 aaaactcggt cccgaagaaa taacaaggga tattccgaac gttggagatg attctctaaa
     5401 ggatttggac gaaagaggaa ttataagaat cggtgctgaa gttgaagcag gtgatattct
     5461 cgtaggaaaa gtaattccta agggtgaaac ggagcttact gctgaagaaa gacttttaag
     5521 ggcgatattc ggcgaaaaag caagagaagt aagagatact tcccttaagg ttcctcacgg
     5581 tgaaagcgga attgttgttg atgttaaagt atttacaagg gaaaacggcg atgagctaag
     5641 ccccggagta agcaaacttg taagagttta tatagctaca aaacgtaaaa taaatgtagg
     5701 cgacaagatg tgcggaagac acggaaataa aggtgttatt tcaatgataa tgcctgaaga
     5761 ggatatgccg tttttaccgg acggaactcc gcttgaagta gtattgaatc ctcttggtgt
     5821 tccttctcgt atgaatattg gtcaagtttt ggaagtccac cttggattgg ctgccaaaaa
     5881 actgggctgg gaagttgcaa cgccggtatt tgacggtgca gtcgaagatg acatatggga
     5941 tgctcttgaa atggcaggct atccgcgaag cggcaagatt gatttaagag acggtcgttc
     6001 aggagaatat ttcgagggac ccgtaaccgt gggatatatg tatatgctta agcttcacca
     6061 tcttgttgac gataaaatac atgcgcgctc cacgggacct tattcattgg ttactcagca
     6121 gcctttaggc ggaaaagcac aatttggagg tcaaagattt ggagaaatgg aagtttgggc
     6181 tttggaagca tatggtgcgg ctcatacatt gcaggaaata ttaacggtaa aatccgatga
     6241 tgttaacggc agggttaaaa catatgaagc aatcgtaaaa ggcgaaaata ttcctgaacc
     6301 cggaatccct gaatctttca aggtacttgt aaaagaatta cagagtttgg ctttggatat
     6361 aaaaattatt accagtgatg atgaagttga aataatcgaa actatcgata atgatgatga
     6421 tgatttacct attgattctg aaagcttagg cgatttggca ttgattgaag gagacatgga
     6481 aggcatgcag attgatagtg acgacataga agatattgac gatattgacg atttagatga
     6541 tttaggtgat atagatgata ttgattctga tgagttagat gagttggaca ttgaagagga
     6601 ttttgacgac gaagataagt tttaaatgaa tggagataca cctctatgta gagcaagtct
     6661 ttggagcagg catagaggaa tgtccccgga cagttggtta catcgaacaa caaaacaagt
     6721 accgattaaa aactaaaaaa gaagggagcg aactccttga tagattacaa taatttcgaa
     6781 tcaataagaa tcgggttagc ttctcccgat aaaatcagac aatggtcaag aggagaagta
     6841 aagaaacctg agacaataaa ttacagaacg ctaaagcctg aaaaagacgg acttttttgc
     6901 gaaaaaatct tcggtcctac aaaggactgg gaatgccatt gcggcaaata taaaagggta
     6961 agatacaaag gtgtagtttg tgacaggtgc ggagttgagg ttacaaaggc aaaagtaaga
     7021 agagaaagaa tggggcacat cgagcttgct gctcctgttt ctcacatatg gtatttcaag
     7081 ggtattcctt caagaatggg ccttattctt gatatgtctc caagagcact tgaaaaagta
     7141 ctttactttg ctcaatatgt tgttacggaa gcaggggata catccttgtc cgaaaaacaa
     7201 ttattgaccg aaatggaata cagagaatac aaggagcatt ataagaacgc atttaaagct
     7261 gaaatgggtg cagaagccat taaaaaactt cttgaaaaaa ttgacctgga aagcgaagct
     7321 gccgaattaa aagctcagtt aagagacagc aagggacaga aaaaaattcg tattacaaga
     7381 agattggaag taattgaagc tttcagagct tcaggcaacc gtccggaatg gatgattttg
     7441 gatgttatac ctgtcatccc accggagcta agaccaatgg tacaacttga cggaggtcgt
     7501 tttgcaactt cagatttaaa tgacttgtac agaagagtta taaacagaaa taatcgttta
     7561 aaaagactcc ttgatttagg agcacctgat ataattgtaa gaaatgaaaa aagaatgctt
     7621 caggaagcag ttgatgcttt aatagataac ggaagacgag gaagacctgt aacaggtccg
     7681 ggaaacagac ctttaaaatc actatctgat atgcttaaag gtaagcaagg ccgtttcagg
     7741 caaaaccttc tcggtaagcg tgtggattat tcaggacgtt cggttatagt cgttggaccg
     7801 gagttgaagt ttaatcagtg cgggcttccg aaaagaatgg ctcttgaatt atttaagcct
     7861 tttgttatga aaaagcttgt tgaaacggga gctgcccaca atattaaaag tgcaaagaaa
     7921 aaggttgaaa aggttgatac tgctgtatgg gatgtactgg atgaaataat caaagaccat
     7981 accgtattgt taaaccgtgc accgactttg cacagacttg gaattcagtc ttttgagcct
     8041 gtacttgttg aaggaaaggc tataaagctt catcctttgg tatgtaccgc atataatgca
     8101 gactttgacg gagaccagat ggccgttcac gttcctttat cggtggaggc acaggcagaa
     8161 tcaagatttt tgatgatgtc cgtgaataat attcttgctc ccaaggatgg aagaccaatc
     8221 acaactccta cacaggatat ggttcttggg tcttattaca tgactttaga aattgaagat
     8281 tctaaaggcc aaggaatgat tttcaaggat tatgatgaaa tgaaaatggc atatttcaat
     8341 aaatttattg atttacatgc taaggtgaag gttagaagga ctgtcggcaa agaatccaag
     8401 ttggttgagt ctacagtggg taggtttata tttaatgaag gaattcctca ggatttagga
     8461 tttgtagata gagaaaaaga taaattttca ctagaaattg ataaaaaagt taccaaaaaa
     8521 atattggaag aaattatcta ccgctgcttc agaaaatatg gaaatgccag aacggctgaa
     8581 gttttggacg gaattaaaca aaaaggtttc agatactcta ccttgggggc tattaccatt
     8641 tcagtaagcg atattacggt tcccgaagaa aagaaaactc ttcttgaaca agccgaaaaa
     8701 caggttctgg aatatgaaaa gaagtataga agaggcttga tgacagatga agaaagatat
     8761 gaaaatgtta taaaaatctg gaatcagaca acagaagacg taacgaatgc attgatgaat
     8821 aatttaagcg aaattaaccc gatttatatt atgtctgtgt cgggagctcg tggaggacgt
     8881 aaccagataa aacagcttgc aggtatgcgt ggtcttatgg caagtgccat aggaaaaaca
     8941 gttgaggttc ctgttaaatc aaacttccgt gaaggtcttt cggttctgga attcttcctg
     9001 tcggctacag gagctcgtaa aggtttaacg gatactgctc ttagaacggc tgactcggga
     9061 tacttgacaa gaagacttgt tgacgtaagc caggatgtca tagtaaggga ggcagactgc
     9121 ggaacttcgg atggcattga ggttgaagac tttatagacg gcaaggaaga aattgaaaga
     9181 ttaagtgaaa gatgtgccga caggtttgct gcagatgatg tagtaaatac tgaaacaggt
     9241 gaaatatttg ttaaagcagg cgagtttatt tctgaagaga tggctataaa aattcaaaat
     9301 tccggaatta aaaaagtcaa ggtaagaagt gttctcactt gtaaaacaat ccacggagta
     9361 tgtgcaaaat gttacggtac caatcttgcc accggaaagc tcgtcaatgt gggagcagca
     9421 gtgggcatta ttgctgcaca gtcaatcggt gaaccgggta cacagcttac aatgcgtact
     9481 ttccatacgg gaggtattgc ggcagctgca gatatcactc aaggtcttcc tcgtatcgaa
     9541 gaattatttg aagcgagaaa accgaaaggt caagctataa tttcagaaat tcccggaaag
     9601 gtaacattgt cggaaagagc gcacaaaaga gaagttacga ttaccaatta cgaagataaa
     9661 gaaagcaaga catacttaat accatacggc tcaaaacttc tggtaaacga aggcgattat
     9721 atagaagcag gtgacaaaat aaccgaaggg tcaatcaatc ctcatgatat tttgaacatt
     9781 aaaggtatga aggcattgga atcatacatt ttaatggaag ttcaaagagt ttacagaatg
     9841 cagggcgttg acatcagcga caaacacgtt gaagttataa tcaggcagat ggtgaacaaa
     9901 ataagaattg aagaatccgg agatacatca ctgctgccgg gaagcttagt ttcaaattat
     9961 gaattttatc agcaaaatga agcagccata aaagaaggta aagagcctgc aaaaggtgag
    10021 attgcacttt tgggtataac caagtcatct cttgcaacag attcattctt ggcttcggca
    10081 tcattccagg agaccacaag ggttcttacg gatgcagcaa caaaaggaaa gattgataat
    10141 cttatcggac ttaaagaaaa tgtcatcatc ggtaaactga tacccgcagg tacagggatg
    10201 aaaagataca gaaatatagg cattactgtt aatgatattg aaaaaccgga tgataatact
    10261 gaaaaaacag atgatgatga aaaatcaatt aaagatgccg gttaaaagca tcattatatg
    10321 aggaagtact tcaaaagtgc ttaaattgca aaaaatattt gacatctaaa aaacaagatg
    10381 atagaatact ttagt
//
LOCUS       DAOPUE010000064        10286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6559_length_10286_cov_11.457727, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000064 DAOPUE010000000
VERSION     DAOPUE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6559_length_10286_cov_11.457727"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      132..263
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            333..965
                     /locus_tag="PKH42_06665"
     CDS             333..965
                     /locus_tag="PKH42_06665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA20151.1"
                     /translation="MEVMSTLQDYDVSQFIQIPDLVKSRKATPKDLSDIYKVACSVGS
                     KSKESYAGFLMDDYKSRPKYYQKFFLERIFELDYFYVAESNIGIVGFLMAYTKEQWLK
                     YNPTWIEDIMWRPDFSMEKTSDFVIVDKTAIMANLTGKGIGSTLYKALLKDLKKKNIK
                     HIFAETLVDPVPNFASLAFRQKQKYTLAGTRYENYMDTLYTDLIYYKPVN"
     gene            complement(1003..1572)
                     /locus_tag="PKH42_06670"
     CDS             complement(1003..1572)
                     /locus_tag="PKH42_06670"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOA20152.1"
                     /translation="MYTIDELKKFCYRCHKCRLAETRNNVVFGEGNENADIMFVGEGP
                     GYYEDIQGRPFVGRAGQLLDKMIEAIGFKRNDVYIANVVKCRPPENRNPFDDECSACL
                     DYLRWQVKLIKPKIIVCLGAVSAKNLISPDLSISRQRGEFVIKGGIYFMPTYHPSYLL
                     RNESAKKDSWNDFKNIAAKIEEIRGTALD"
     gene            complement(1582..1923)
                     /locus_tag="PKH42_06675"
     CDS             complement(1582..1923)
                     /locus_tag="PKH42_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA20153.1"
                     /translation="MNDNAPYKNNYDSCPLTFALTLIGGKWRLPVIWSIHKNKTMRYN
                     ELRRDIQGITSMMLTQSLKELEAYGIVERKQYAEIPPRVEYSLTDNGENLIPALKALA
                     DWGDKMRKLKA"
     gene            2053..2601
                     /locus_tag="PKH42_06680"
     CDS             2053..2601
                     /locus_tag="PKH42_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA20154.1"
                     /translation="MGLSIQYEIVSEDNIFIIKDLCNELMAYQKSKAYIHPEFFDNMS
                     FETRVLPAMKSSKENYIIAANDGDEVVGYAYSTIAPKTIYSGGFATLQCDAFFDFDSV
                     ETEDVGCLSQFFIKENYRNTGIGTAMFNVSMDWLNSFKEIKDIFIFVSNGNENALNFY
                     LNKGFKVSHEILDGFITVLRNK"
     assembly_gap    2604..2703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2966..3403
                     /locus_tag="PKH42_06685"
     CDS             2966..3403
                     /locus_tag="PKH42_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20155.1"
                     /translation="MNNDQNTVEILSEIYRGAKMGVETINSMLNKVNDNKIYDELKYQ
                     LRSYDEIANEAYGELVKRNQEPKDISTVNKLSAKMSVGINTMISNTPSHIADMLIKGS
                     TMGVTEMTKSLNSYKDADPDIQALADRFIKLEQDNIDRLMKFL"
     gene            complement(3454..4320)
                     /locus_tag="PKH42_06690"
     CDS             complement(3454..4320)
                     /locus_tag="PKH42_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOA20156.1"
                     /translation="MLNELNRVIDYIEEHLTDDELSLEIISEYAGVSDYHFRKIFFYL
                     SGMTLSEYIKNRKLSEASMDLLHGEKVTDVAFKYGYQSMDGFTRAFKKWCGLLPSEVI
                     KNGISKSFPKLSFIITVKGGINMEFRIENKPAFNLVGVCKRVPMQFEGINNEIVKLAE
                     SITDEQREEMHSLQNIEPYEIVNASYEADADFLKEEGYLTHLIGVLTTKNEISDLLEK
                     VPVEAHTWAVFPNEGPFPSTLQETYAKIYSEWLPSSGYEVINAPTFSFTKMDKNKENY
                     AYSEVWTPIRKK"
     gene            complement(4409..5491)
                     /gene="rsgA"
                     /locus_tag="PKH42_06695"
     CDS             complement(4409..5491)
                     /gene="rsgA"
                     /locus_tag="PKH42_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOA20157.1"
                     /translation="MSKINMENIGLSQRFKNESTLYEGLYIGRIASQYKGLYKVITED
                     GILTAEVSGKFRFDAKTTSDYPAVGDFVMIDRNDDKDGNAIIHHVLTRKSAFVRKAAG
                     TSNEEQIVASNIDTVFICMSLNNDFNIRRIERYLGIAWDSGASPVIVLTKSDLCDNIS
                     EKLAELDTVAIGADVIVTSSMSEDGYLPIKRYLGSGKTIAFIGSSGVGKSTLINKLIG
                     EDVLETNGLRNDDKGRHTTTRREMLLIPDGGVVIDTPGMREIGIESADLTKTFADIDE
                     LSAKCKFSDCTHINEPKCAVQEAISNGLLSRDRFESYLKLKKEAKYDGLNSRQIENKK
                     LNEMFKDFGGMKNVRKMLKEKKKLER"
     gene            complement(5623..5808)
                     /locus_tag="PKH42_06700"
     CDS             complement(5623..5808)
                     /locus_tag="PKH42_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOA20158.1"
                     /translation="MKKITAIIGSARKGATYQAVREFEQNLKEYEEIDFEYIFLSDYN
                     LEFCRGCKICFDKGEAY"
     gene            complement(5901..6410)
                     /locus_tag="PKH42_06705"
     CDS             complement(5901..6410)
                     /locus_tag="PKH42_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20159.1"
                     /translation="MKSINKMRNYYLVFFVIAALLTLWFALKFIYEAAFIFGNISIVL
                     LLYVIKQNRLLYDTRLIYDNCIFKIPSATVSTSNSEIKLAVEEIVVSAFGLIIGNKIY
                     KWGYHGAYGVKLKAIEINPERVYLTFGDASKTARVELLHGITNKQEVLDIKQKFWRET
                     GIDTVVNGW"
     gene            complement(6455..8437)
                     /locus_tag="PKH42_06710"
     CDS             complement(6455..8437)
                     /locus_tag="PKH42_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20160.1"
                     /translation="MKNKIISLLLISSIIFNMVGFSGCSKAEEKLVDTSVQLPEHITG
                     TVSDENLTVSQDGVSFTIEEGSVLMDTDVEITLKKDLPPIIVGDEEIMLTAYDFSVAG
                     EEPLGVMTLEIPFTASDDEVFAAYYNLEKGEWEPVEFIYDAERKCAVIYTTHLSSYGL
                     FTVENENTRNALIAYTYNLELAEVNINEAIRMLENAMEDPDTFYEDCFDLISNSVSIH
                     SSITTNLVDVVSGLSSYVPTFSEGLKDNVTKFGYLLTGIEVVNAFMNDDKPAAVWKMF
                     DTAVGFNVDKIASLSGTASMAAGLFSVGLVSYALNKFISTALQGRTDMYERAYNLYYK
                     ENGRTTAEWVYLIRAMVENGKDPEKTGEAISDEIKRYVNEFWEDDLTIAEYGKDANPN
                     AWSSGSLGGLNDGIKKEISNYYQMTIERVVGNILNRIQIENKINMQSEMRKELFNFQK
                     KMNSIVTISVKDSKAENEPSHFAGAVIRFSELPSSIEDPEKWESKISEKGAARIQFRV
                     LAHLLAGSPRKMEIVKKSGDKEDIMVRFNAKFSLPESKLDLADLYPRSDYPKNIGGSY
                     TVSMYNTTTGKTIDDLMLKVTVTQEVSDGCYADFYLENSGSVYLNGNYFYRYATGKVN
                     YALGDMSFSGDDNNVSFKFDAYDNQKKVWGAFTGHK"
     gene            complement(8646..9944)
                     /locus_tag="PKH42_06715"
     CDS             complement(8646..9944)
                     /locus_tag="PKH42_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOA20161.1"
                     /translation="MREIDASTYIMAAIIAHFAGLLTCGFFVKSHIAAKKMIVAGAIL
                     CLVVTIPFFFTPSALWMGSLVIGGFINGCAVAAWGYFLRAFTPKNERIKTCADVLIYS
                     NIIMIALNVIAMNRAPLIGLILSMFCLVIGAAFILMLPAESTYENKILNNNVGGGIKN
                     PMILLCIFVFIITINSGLMYQVINPAFDHLTGFVSWYWAVPYIIALAIMRNLPEKANR
                     SIILYIGMAMIMGAFITFMILNRSIFDYFIVDTLMLGACGIFDLFWWSILGEMLDYTE
                     NPAMLFGIGLSSNVLGVLCGGFIGVFVTSIGLPDAEVAVIALTMVCITLVMLPPLNNQ
                     LVLLLKNHAYLAVYDNMSRSQQTDIIRYAKTLDPLTVREEEVLQLILTGKSNREIAEL
                     LFISENTVKTHARNIFSKYDVGSRAELISTLLKNQVSSIL"
BASE COUNT         3216 a         2029 c         1550 g         3391 t
ORIGIN      
        1 ataaagaata agttcgacaa gatgcaacac gtacaccact tagaattgca ggtctacatg
       61 atttttcaaa atttattctt tacaataaat caaaaatatg gtatacttct atgaagtaga
      121 ttgtaatatt gtaaattgta acgctgaatt ttttgtatga aaaagcgggg aaaccaattt
      181 tttggggtga atctctatga gtagggttaa cttctttcga cccgaacccg tcagctaacc
      241 tcgtaagcgt tggaagagga aaatgaaaca aataactgca tatatcattt tcctattttt
      301 gcaatcgcta attttaaagg gaggatgaca atatggaagt tatgagcact ttacaagatt
      361 atgatgtaag tcaatttatt caaattccgg acctagttaa gtcaagaaaa gcaacaccga
      421 aagatttatc agacatctat aaagttgcat gctcggttgg ttcaaaatca aaagaatctt
      481 acgcaggttt ccttatggat gactataaat cgagaccaaa atattatcaa aaattcttct
      541 tggaaagaat ttttgaactg gattactttt atgtagctga aagcaatatc gggatagtag
      601 gttttttaat ggcctacacc aaagaacaat ggttaaaata caatcccact tggattgaag
      661 acattatgtg gcgaccggac ttctcgatgg aaaaaacttc tgactttgtg atagttgaca
      721 agacagctat aatggcaaat ctaaccggta aaggtatagg aagcacactt tataaagctc
      781 tcttaaaaga tttgaaaaag aaaaatataa aacatatatt tgcagagacg cttgtagacc
      841 cggtacctaa ttttgcttct ctggctttta gacaaaagca gaaatatact ttagccggca
      901 cacgttatga aaattatatg gatactctat atactgactt gatttattat aaaccagtaa
      961 actaatcctt aatgactcta tcaagagtca ttttttattt gcttaatcaa gggctgttcc
     1021 acgtatttcc tcgattttag cagcaatatt tttaaaatca ttccatgaat ccttcttagc
     1081 tgattcgttt cttaacagat atgacggatg atatgtaggc ataaaataaa tgcctccctt
     1141 aattacaaac tctccccttt gcctgctgat acttaaatcc gggcttatga gatttttagc
     1201 ggatactgct ccaagacata caataatttt tggtttaatc aacttaacct gccatcttaa
     1261 ataatccagg catgcactgc attcatcatc aaaaggattt ctgttttcag gaggcctgca
     1321 ttttacaaca ttggctatat atacatcatt tcgtttaaag cctattgctt cgatcatttt
     1381 atcaagaagc tgtcctgctc tgccgacaaa aggcctccct tgaatatcct cataatatcc
     1441 cggaccttcc cctacaaaca ttatgtcagc attctcattt ccttctccaa aaacaacatt
     1501 atttcttgtt tcagctaatc tgcacttatg acatctatag caaaactttt ttagttcatc
     1561 aatcgtatac ataatacctc ctcaggcttt cagcttcctc attttatctc cccaatcagc
     1621 caaggcctta agagccggga taagattttc tccgttatca gttaaggaat attctaccct
     1681 gggcggtatc tcagcatact gctttctctc gacgataccg taagcttcca attcctttag
     1741 cgactgggtt aacatcatgc ttgtaatacc ctgaatatct cttcttaact cgttataacg
     1801 catagtttta tttttatgaa tagaccaaat aacaggcaat ctccactttc cgcctatgag
     1861 agttaaggca aatgtaagcg gacagctgtc ataattattc ttataaggtg cattgtcatt
     1921 catttaacca ctccattact cagttttttc ttacttagac ataaaatatt gcatacttgt
     1981 tacatttttt ttagctgata taattataat cgaagattaa ttaatcagtc aaatttttat
     2041 tacgaaagga tgatgggttt gagtatacaa tatgaaattg tttcagaaga taatattttt
     2101 attattaaag atttatgcaa tgagctaatg gcttatcaaa aatccaaggc atatatccat
     2161 cctgaatttt ttgacaatat gagttttgaa acaagggtgc taccggccat gaaaagctca
     2221 aaagaaaatt acataatagc agctaatgac ggcgatgaag ttgtcggata tgcttacagc
     2281 acaattgcac cgaagacaat atactccggc ggctttgcta ctttacaatg tgatgccttt
     2341 tttgattttg attctgtgga aacggaagat gtaggatgcc tctctcaatt ttttataaag
     2401 gaaaattatc gcaataccgg tatcggtacg gcaatgttca acgtgtccat ggattggctg
     2461 aattcattta aagaaataaa agatatattt atctttgtat caaacggaaa tgaaaatgca
     2521 cttaattttt atctgaataa aggtttcaag gtaagccatg aaattcttga cgggtttata
     2581 accgtattga gaaataaata atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnntatttat cttttgattt tttacttttg tcgctcatgc tgaatctcct tctgtatttt
     2761 atttatagta tgtcatgaaa agagaaattt taaacaaaat ttccccctta aaatgagaat
     2821 gctaagtaag tattttataa ctgcattaaa tgctcaaagt tttattttat attattgcct
     2881 tagatcatca atctattatc atttactaag tttttgtatc agaattttaa tattgtaaat
     2941 aataaactaa aaaggaggta aacaaatgaa taatgatcaa aatactgtag aaattttatc
     3001 cgaaatttat cgcggagcta aaatgggcgt tgaaacaatt aacagtatgc taaataaagt
     3061 taatgacaac aaaatatacg atgaacttaa gtatcaactt agaagttatg atgaaattgc
     3121 taatgaagcg tatggcgaac tggtaaaaag aaatcaagaa cccaaagata tttccaccgt
     3181 gaataagctt agcgccaaaa tgagcgtagg tattaatact atgattagta atacaccatc
     3241 tcatattgcc gatatgctta tcaaaggcag taccatggga gttacggaaa tgactaaaag
     3301 cttaaattcg tacaaagatg cagaccctga tattcaggcg ctggcagaca ggtttataaa
     3361 attggaacaa gataatattg acaggttaat gaaattctta taataaaccc agacaaaaaa
     3421 cccccacttt taaatagtgg gggttttaaa tgcttatttc tttcttatgg gagtccaaac
     3481 ttcgctgtag gcgtaatttt ctttattttt atccattttg gtaaaagaaa aagtaggagc
     3541 gtttattact tcatacccgg aagatggaag ccattccgaa tatatttttg catatgtttc
     3601 ttgtaacgta gacggaaatg gtccttcatt cggaaacacc gcccatgtgt gagcttcaac
     3661 aggaaccttt tccaataaat cactaatttc attttttgtt gttaacacac ctatcaagtg
     3721 ggttaaatat ccttcttctt ttaagaaatc agcatctgct tcataagaag cattgactat
     3781 ttcataaggt tcaatattct gaagagaatg catttcttct ctttgctcgt cagttatact
     3841 ttccgcaagc tttacaatct cgttatttat tccttcaaat tgcatgggga cacgcttgca
     3901 aactccgact aaattaaacg ccggtttatt ttcaattcta aattccatat taattcctcc
     3961 tttgactgtt attataaatg aaagtttggg aaaggacttg cttattccat tttttattac
     4021 ttctgaaggc aataatccgc accacttttt gaatgctcga gtaaaaccgt ccattgactg
     4081 ataaccgtat ttaaaagcaa catccgttac tttttctcca tgcagtaaat ccatgctcgc
     4141 ttccgacaat tttctgtttt tgatatactc gctaagggtc attcctgaaa gataaaagaa
     4201 tatctttcta aagtgatagt cggaaacccc ggcatattca gaaattattt caagagatag
     4261 ttcatcatcg gttaaatgtt cttcaatata atcaattaca cggtttaatt cgtttaacat
     4321 tcattcccca tccttcgtct tcataatacc aaagatattt ttataatgct cgattttttt
     4381 attataaaaa atatcgaatc aaaggatatc aacgttctaa ttttttcttc tctttcaaca
     4441 tctttcggac attcttcatg ccgccaaaat ccttgaacat ttcattaagc tttttattct
     4501 cgatttgcct ggaatttaat ccatcgtatt tagcttcttt ttttagtttt aaataacttt
     4561 caaatctatc tcttgacagt aagccgttac ttatggcttc ttgcacagca cattttggct
     4621 cattaatatg cgtacaatcg ctaaacttgc acttagcgga caactcatca atatcggcaa
     4681 aagtctttgt taaatcagca ctttcaatac ctatttccct cattcccgga gtatcaataa
     4741 caacaccgcc gtcaggtata agaagcatct ctcttctggt cgttgtatgt ctgcctttat
     4801 catcatttct tagcccgtta gtttcaagga catcttcacc gataagtttg ttaatcaagg
     4861 ttgacttacc gacacctgaa gaacctataa aggcaatggt ttttccgctg ccaagatatc
     4921 ttttaatcgg taaataacca tcctcgctca tgcttgaagt aactattaca tctgctccga
     4981 tagcaacagt atcaagctct gccaattttt cagagatatt atcgcataaa tcggatttgg
     5041 taagcactat aaccggactt gctccgctgt cccatgcaat ccctaagtag cgctcaattc
     5101 tacgaatatt gaaatcatta ttaagcgaca tacaaataaa aacagtatca atattggaag
     5161 caactatttg ctcttcattt gatgttcctg cagcttttct gacaaaggca ctttttcttg
     5221 taaggacatg atgaattatt gcatttccat ctttatcgtc atttctgtca atcataacga
     5281 aatcccctac agccggataa tcagatgttg tttttgcatc aaaacgaaat ttccctgaaa
     5341 cctctgctgt taatatgcca tcttctgtta taaccttgta taaacctttg tactgagaag
     5401 ctattctccc aatataaagt ccttcatata aagtagactc atttttgaac ctttggctga
     5461 gtcctatatt ctccatattt attttactca attttattcc tcctaatatt catattatgt
     5521 tatgactttt tggaggttta tactttatta ttttgtatgc acctccccat ttactgcaat
     5581 atccgttact atcttattaa acatgaatac caactccttt catcaatacg cttctccctt
     5641 gtcaaaacaa atcttacatc ctcggcaaaa ttcaagattg tagtcactta agaaaatgta
     5701 ttcaaaatca atttcttcat actcttttaa attctgttca aattccctca ctgcctgata
     5761 agttgctcct tttcttgcgc tgcctataat tgctgttatt tttttcacaa tattccccct
     5821 gacaacaaat actttagatt aaaacacttt cttataccta tatgcattat aacatattct
     5881 tccataatca agaagttttt ttaccaaccg ttaacaactg tatctatacc tgtttctctc
     5941 caaaacttct gctttatatc cagcacttcc tgcttatttg tgatgccgtg caataattcg
     6001 accctcgcag tttttgatgc atcaccaaag gttaaataaa ctcgctccgg attaatctca
     6061 atggctttca attttacgcc atatgctccg tgataacccc atttgtaaat tttgttgcct
     6121 ataataagtc caaaagcaga caccacaatt tcttcgaccg ctaattttat ttcactgttt
     6181 gatgtggaga cggttgctga tggtatttta aaaatgcagt tatcataaat gagcctggta
     6241 tcgtacagca gtcggttttg tttaattaca taaagaagaa gcacaatact tatattccca
     6301 aatataaacg ctgcctcata aataaacttc aaagcaaacc atagagtcaa caaagcagca
     6361 attacaaaaa atacaagata ataattacgc attttattaa tagacttcac ttgtatcaca
     6421 ccccttttat gtctattctg ccctgtaaag agtgttattt atgtccggta aaggcccccc
     6481 ataccttttt ttgattgtca tatgcatcaa atttaaagct tacattgtta tcgtcacctg
     6541 agaaactcat atctccaagg gcataattga ccttacctgt tgcatagcga taaaagtagt
     6601 ttccattcaa atataccgag cctgagtttt caagatagaa atcagcataa caaccgtcgg
     6661 aaacctcttg tgtaacggta accttcagca tcaaatcatc aattgttttt cctgtagttg
     6721 tattgtacat tgaaacagta tagctccccc ctatattttt aggataatct gatctcggat
     6781 aaaggtcagc taaatcaagt ttggattcag gcaaactgaa ctttgcattg aatctcacca
     6841 taatatcttc tttgtcaccc gacttcttca ctatttccat ctttctcgga ctgccggcta
     6901 aaagatgagc caatactcga aattggattc ttgctgcacc cttttctgaa attttacttt
     6961 cccatttctc cgggtcttca atacttgaag gaagctcaga aaatcttatg acagcccctg
     7021 caaagtgcga cggctcattt tctgccttgc tgtcttttac tgatatagta actatggaat
     7081 tcattttttt ctgaaaatta aataattctt tacgcatttc tgattgcatg ttgattttgt
     7141 tttctatttg aatacgattt agtatattcc ctacaactct ttcaattgtc atttgatagt
     7201 agttacttat ttctttctta ataccatcgt taagtcctcc taatgagcct gatgaccatg
     7261 cattagggtt ggcatctttg ccatattcgg caatagtaag atcatcctcc caaaattcat
     7321 taacatatcg cttaatctca tcagaaattg cctcgccggt tttctcgggg tccttgccgt
     7381 tctctaccat tgctctaatt aaataaaccc attctgcagt tgttctgcca ttttccttat
     7441 aataaagatt atacgccctt tcatacatat cggttcttcc ttgtaatgcg gttgaaataa
     7501 atttatttaa tgcatagctt acaagtccga ccgagaataa acctgccgcc atagaggcag
     7561 taccgctcaa ggatgcgatt ttatccacgt tgaatcctac tgctgtatca aacatcttcc
     7621 atactgcggc cggtttatca tcattcataa atgcattcac aacctcaata ccggtcaata
     7681 aatatccaaa ttttgtaaca ttatccttca atccttcact aaaagtggga acatatgaag
     7741 acaatccgga cacaacatcc accagattag ttgtaatact gctatgaata gaaacactgt
     7801 ttgaaattaa atcaaaacaa tcttcataga aggtatcagg gtcttccata gcattttcaa
     7861 gcattcttat tgcctcgttt atattgactt ccgccaattc caaattgtag gtgtaagcaa
     7921 tcagcgcatt tcgtgtattc tcgttttcaa cagtaaatag accataggaa gataaatgag
     7981 tggtatatat aacagcgcac tttctttccg catcatatat aaactctacc ggctcccatt
     8041 ctcccttttc caaattgtaa taggcggcaa aaacctcatc atctgaagca gtaaaaggaa
     8101 tttccaacgt catgacacct aagggttcct caccggcaac gctgaaatca taagcagtga
     8161 gcataatttc ctcgtctccg actataatcg gaggcaaatc cttttttaaa gttatttcta
     8221 catcagtatc cattagcact gatccttcct ctatggtaaa tgaaactcca tcctgagaaa
     8281 cagtcaagtt ttcatctgat acggtacctg ttatatgttc aggcagctga acagatgtat
     8341 caacaagctt ttcttcggct ttactgcaac cggaaaagcc gaccatatta aatattattg
     8401 aactaattaa tagtagagat attattttat ttttcatttt gtcctccttt ttgttatcct
     8461 tggcatatag tttttcctcc tttataatgt tatgagattt ttgtatatga gagttttgat
     8521 acatcctctt atattggata ttttaagaga gaaaccttaa taaagcatcg ttcgaaagga
     8581 tgaatttaca aaaaaaacct catactttag tatgaggggt tctatttatc aatatttata
     8641 taaacttata gtatagaact gacttgattt ttgaggaggg tgcttattaa ctctgcccta
     8701 ctgcctacat catattttga aaaaatgttc cttgcatgcg tctttacggt gttctcgcta
     8761 atgaataaaa gctcggcaat ttctctgtta gacttacctg ttaaaataag ctgcaacacc
     8821 tcctcttccc gaactgtcaa cggatccagt gttttagcat atcggatgat gtccgtttgc
     8881 tgtgaccggc tcatattatc ataaaccgca agataagcat gatttttcag cagcagtaca
     8941 agctgattgt taagtggcgg cagcataaca agagtaatgc agaccatggt aagagcaatt
     9001 accgccacct ccgcatcagg aagtccaatg gatgtcacaa acactcctat gaaaccgccg
     9061 caaagaacac ctaacacatt tgaagatagt ccgatgccaa acagcattgc cggattctca
     9121 gtgtaatcca gcatttcacc aaggatgctc caccaaaata aatcaaaaat tccgcatgca
     9181 ccaagcatca atgtatcaac aataaaataa tcgaaaatgc ttcggttcaa aatcataaaa
     9241 gttatgaatg ctcccataat cattgccata cctatataca atattattga acgattagcc
     9301 ttttcgggca ggtttcgcat aatagctaag gctataatat aaggtaccgc ccaataccaa
     9361 cttacaaatc ccgtcaagtg gtcaaaagca gggtttatta cctgatacat caaccctgaa
     9421 tttattgtga tgataaacac aaaaatgcac aagagtatca ttggattctt aatgccgcca
     9481 cctacattgt tattcaatat tttgttttca tacgtcgact ctgccggcag catcaatata
     9541 aaagctgcac caattacaag acaaaacatg gaaagaatta atcctatcaa gggtgctcgg
     9601 ttcatggcaa tcacattgag cgcaatcatt attatattgg aataaatcag cacgtcagca
     9661 caggttttaa tacgctcatt tttgggagta aatgccctaa gaaaatatcc ccatgctgcc
     9721 actgcacaac cgtttataaa tcctccaata actaagcttc ccatccataa ggccgatgga
     9781 gtaaaaaaga aaggtattgt gacaactaag cataaaatcg caccagctac tatcattttt
     9841 tttgcagcaa tgtgagattt aacaaaaaaa ccgcaagtta aaagacccgc aaaatgtgct
     9901 ataattgcag ccattatata agtggaggca tctatttctc gcatattcaa taagctatac
     9961 agcacctgtc cttcaaattg gaaagataaa atataagcaa ataggaacga gaatcctgca
    10021 atagaaagtc tacgcgtatt aattagtttc catctcttgc tatgtgtatt ttcgtgcatg
    10081 gattatacct ttttatttat atttatgcaa gtattttata attacttttc taattgtagc
    10141 ataagaatct tttttgttca atactatttg caaaactgtt actacaccaa actcttcacg
    10201 caatacaaaa attccctcaa aattatcttg agggaatggt acaaagtatt agagattata
    10261 tttatcaata tatataactt tataat
//
LOCUS       DAOPUE010000065        10269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6571_length_10269_cov_11.902976, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000065 DAOPUE010000000
VERSION     DAOPUE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10269
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_10269_cov_11.902976"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PKH42_06720"
     CDS             complement(<1..473)
                     /locus_tag="PKH42_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA20162.1"
                     /translation="MEQKVIMAGFGGQGIMAIGRLLAYAGMLEDKNVTWMPSYGPEMR
                     GGTANCAVVVSEEEIGSPLISKDGTAAIVMNLPSMIKFEKELVPGGKLIVNKSLIDVE
                     PTRNDLDVYYIPANELALELGNGKVANMVMLGAYLEITKAVEIESVLKAFVKVFG"
     gene            complement(475..1221)
                     /locus_tag="PKH42_06725"
     CDS             complement(475..1221)
                     /locus_tag="PKH42_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA20163.1"
                     /translation="MTIVYEKSKGLTDTPFHYCPGCTHGVIHKLVAESLVELGVLDDA
                     IGVAPVGCSVLAYKYFNCDMQEASHGRAPAVATGIKRVHPDKVVFSYQGDGDLASIGT
                     AEVVHAAHRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKTTTSPFGRDEAHCGKPIKM
                     SELLATIDGAKFVERVTVDTPANIRKAKKAIKRAFECQIKGEGFAIVEVLSSCPTNWG
                     FTPLKALDWLRDNMIPYYPLGNFRDFGEGK"
     gene            complement(1221..2282)
                     /locus_tag="PKH42_06730"
     CDS             complement(1221..2282)
                     /locus_tag="PKH42_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOA20164.1"
                     /translation="MSKVLMKGNEAVGAAAIKAGCKCFFGYPITPSSEIPEYISRKLP
                     EAGGVFLQAESEIAAINMVYGAGGAGARCMTASSSPGISLKQEGITYAAGAEIPCVIV
                     NMMRGGPGLGSIQPSQADYYQSTRGGGNGDYRTLCYAPSSIQELVDYVMLAFDKADYY
                     RNPVLIAADGMIGQIMEAVEFKEPEPIELPPKDWATTGTGGKRKPNVVNSLYLQAEEL
                     QEHNKRLEKKYKIMEENEVRYEAFGLEDAEVVCVAYGTTSRIVKSAIKQAQAKGIKVG
                     MIRPITIWPYPYDVFKEIGPNAKGILGVEMSTGQLLDDIKIGIAGRLPVYFNGTQGGM
                     VPTPGSILADIEKIAGGTK"
     gene            complement(2295..2510)
                     /locus_tag="PKH42_06735"
     CDS             complement(2295..2510)
                     /locus_tag="PKH42_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA20165.1"
                     /translation="MAKGKVTFNDDACKGCELCVAVCPVKIIELNKEKINNKGYSPAH
                     VTDQEKCIACGNCAIMCPDSVITVERL"
     gene            complement(2524..3201)
                     /locus_tag="PKH42_06740"
     CDS             complement(2524..3201)
                     /locus_tag="PKH42_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA20166.1"
                     /translation="MVHDKRLVIVIGHYGSGKTEFAVNYAVKMKEIYENVSIADLDIV
                     NPYFRSREKREFFEEIGIKLFDSSIRNTAVDLPALPAELMGVITNPNIKSIIDVGGDP
                     VGARVLARFAEQIKNVDYDMFYVINGNRPDTSTVDGVINYLKMIEATSRLKITGLVNN
                     THMLKATTVEDVEFGHELTKKVSWETDIPIRYEAVIKETADKIKNHEIIEKLFPINLY
                     MREEWMS"
     gene            complement(3231..4298)
                     /gene="buk"
                     /locus_tag="PKH42_06745"
     CDS             complement(3231..4298)
                     /gene="buk"
                     /locus_tag="PKH42_06745"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOA20167.1"
                     /translation="MKQLIINPGSTSTKIAVFDDVTMIFEKTIRHTSEELQPFNRVVD
                     QFQFRKEMIISSLKENNISLNELDAVVGRGGLLKPIEGGTYLVNDKLIKDLETNIKGE
                     HASNLGALLAHSIADEVNIPSFIVDPVVVDEMEDVARISGHPEFERICIWHALNQKAV
                     ARKAAIEKFGKKYDEVNFVVVHLGGGISVGAHRKGRVIDVNNALNGEGPFSPERTGSL
                     PAAQLVKMCFSGKYTEAEIYKIIAGKGGICAYLGTNSAKEVSDRANDGDDKARLIYSA
                     MAYQVAKAIGEYAVVLDGDVDAIIITGGIAYDKDFVKMIEDKVKFIAKVIVYPGEDEL
                     LALAEGGLRVLKGVEEAKTYN"
     gene            complement(4295..5203)
                     /locus_tag="PKH42_06750"
     CDS             complement(4295..5203)
                     /locus_tag="PKH42_06750"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOA20168.1"
                     /translation="MNRFDELLKMAKTTVPKKIAIAAAQDEDVLMAVKSAYLEKICIP
                     ILIGNKEKIIELSQKINFNLDDIQIIESKDDNEAARTAVSLVSSGKADILMKGLVDTA
                     IILKAVLDKEIGLRTGNILSHAAVFESDRYHKLFIITDAAMNIAPSASEKKQIIENTL
                     PLCRSLNIENPKVAVICAKEKVSPKMQATVDAEILVNMNKNGEIKGCMVEGPFGLDNA
                     ISREAAALKGVKGEVAGDADILLMPNIEAGNVMYKTLTYLADSKNAGIILGAKAPIIL
                     TSRADSDEAKLYSILLAVICSKKESI"
     gene            complement(5361..6749)
                     /locus_tag="PKH42_06755"
     CDS             complement(5361..6749)
                     /locus_tag="PKH42_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA20169.1"
                     /translation="MQKINFNSMDYQGMLEAVFLATQDAISVVDEKGEHILVNPAYTK
                     ITGIEHDDIIGKNALYDIEDGEESVHMKVLSTKKPVENTKLKLRPSGKTVVAQAAPIN
                     VNGKLKGSVAVLHDISTIKDLTDKLEEAEKKLRELTYKYKPEDIIGNSKAINQVRNLI
                     EKAAKVPATVLLRGESGTGKELFANSIHAQSSRSHKNFVRVNCAALTDSLLESELFGY
                     DEGSFTGALKGGKKGLFEEADKGTIFLDEISEISLNTQAKLLRVLQEKEIMRVGSNKN
                     IPVDVRVIAATNADLAKSVREGKFREDLFYRLSILPINIPPLRERIEDIPLLVKSFIA
                     KFNDEYGRNIVDISNDALDVLMSHEWPGNVRELENIIGRAIIYMNINERVIRTTHLPQ
                     LNSNKVQNTVKSTVLTDDDVSTLDRVLSKTESAYIESVYLKFDKNKTRTAKELGISLR
                     SLYYKMEKYGIF"
     gene            complement(6765..7991)
                     /locus_tag="PKH42_06760"
     CDS             complement(6765..7991)
                     /locus_tag="PKH42_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOA20170.1"
                     /translation="MKIKNDKLVIQITCLIVAFVLWMIVMLVTNQTLEQSYPNVSVTI
                     RNLSALEKSNLVIMNQDKENITVTVKATGLTEQLEKISSRDFSAYIDVLGFNEGITNA
                     KVEVVGPSGVDVVGVYPSQIACNIEGVISKVMDVTVQFEGEHAKNYYRAQTVPNPSSV
                     KITGPRSVVNSADKAVATINIEGAMDDVVKTVPVRIYDDTDNEIFLSAPIGNVKVTVP
                     IYPTKYVALEPTITGIPEEGYELVDVTVKPERVRIAAEQDVLDTFKKLTLEELDITGA
                     YNNILSTRNILNAEGLVFLDMETKPVVNAVVEKIIERDLRYSYDDIQFINKIENSDVE
                     IKETEEEITVTVRGTSTLVNSLKKEDLILTLDLSETVNGINTVNLDCISEFTFKEISL
                     SKETVTIEVSKEPLEP"
     gene            complement(7972..8811)
                     /gene="cdaA"
                     /locus_tag="PKH42_06765"
     CDS             complement(7972..8811)
                     /gene="cdaA"
                     /locus_tag="PKH42_06765"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOA20171.1"
                     /translation="MYDIMHRMGLIMSNIGPNTIVDIGIVYYVLYRGYILIRDMRAKQ
                     LVKGIVLLVALIPISQIFELYMVEFILSNTFQVGLIALVIVFQPEIRKALEYLGRTSF
                     TLSNVEKTNETSRKIIKEIISAITSLSRQKIGALIIFEKQIGLNDIIESGTVINANIS
                     SGLLINIFIPNTPLHDGAVIIKDYTIKAAGCFLPLTENNLLSKDIGTRHRAAIGMTER
                     SDSIALIVSEETGYISYAVEGRLYRNIQNEEIESLLQSIYIENEKVNIIDKWRNRNED
                     KKR"
     gene            complement(8826..10091)
                     /locus_tag="PKH42_06770"
     CDS             complement(8826..10091)
                     /locus_tag="PKH42_06770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOA20172.1"
                     /translation="MNRKVRRRKKRIKLMSWSLVLLVGVLAASFVVRGIGKDGKKDKQ
                     PLEEHPEEQIGEEASNEVKEPQPEPDVTVRIRATGDIMFHPSQLDGAYDYKTNTYNFE
                     HSFKAIKSIIREADIAIANFEGTTAGNEVYAYQGYPLFNAPDEALDAIKNAGFDILST
                     ANNHSLDTRKAGIIRTIEQIKARGMEPIGTFTSKPETRVLIKDVKGIRFAFMAYTEMV
                     NGLETVLTPEDLDVMVNIIDEEKMKEDIIYAKDNNADVIIAYLHWGDEYSRIQAQRQE
                     ILADMLFRSGVDIILGSHPHVIQPTQTLDYDGKTKFIAYSMGNFLSNQRVETLVPYGM
                     SEQVSKYTEDGIIIDIEIEKKGSTKEISIKNINYIPLWVYKGVKENGTAEHIVYPIMD
                     YIESAELDEDSKARMSRSYADTMAQMNAE"
BASE COUNT         2806 a         2225 c         1498 g         3740 t
ORIGIN      
        1 tctccgaaaa cttttacaaa agctttcaat actgattcaa tttcaaccgc tttagtaatc
       61 tctaaatatg ctccaagcat aaccatattt gctactttac cgttacctaa ttccaatgct
      121 aactcgttag ccggaatata ataaacatct aaatcgtttc ttgtcggttc aacatcaatc
      181 aatgatttgt ttactattag ttttccgccc ggaacaagct ccttttcaaa tttaatcatt
      241 gaaggtaagt tcataactat cgctgcagtt ccatccttag aaataagagg ggagcctatt
      301 tcttcctctg atacaacaac tgcacaattt gctgtacctc ctctcatttc gggaccgtaa
      361 gaaggcatcc aagttacgtt tttatcttca agcattccag catatgccaa taaccttccg
      421 atagccataa ttccttgacc gccaaatccg gccataatta ctttttgttc catattattt
      481 tccctcccca aaatctctga agtttcctaa aggataatat ggtatcatat tatctctcag
      541 ccagtccaat gcttttaaag gagtaaatcc ccagtttgtc gggcatgatg ataatacttc
      601 tacgattgca aaaccttcgc cttttatctg acattcaaaa gctctcttta tagctttttt
      661 tgcttttctg atatttgccg gagtatctac tgtaactctt tctacaaatt tagctccatc
      721 aattgtcgcc aatagttctg acatttttat aggttttccg caatgtgcct catctcttcc
      781 aaatggagat gtggttgtct tttgtccaac caatgtggta ggagccatct gtccgccggt
      841 catcccgtat attgcgttgt taacaaagat tgtggttatt ttctctcctc tatgtgctgc
      901 atgtacaacc tccgcagttc ctattgatgc taaatcaccg tcaccttggt atgagaatac
      961 aaccttgtcg ggatggactc tcttgatgcc tgttgcaact gccggtgctc ttccgtgaga
     1021 agcttcctgc atatcgcagt taaaatattt gtaagcaagt actgaacatc caacaggagc
     1081 tactcctatt gcatcatcca aaactcctaa ttcaaccaaa gattctgcaa ccagtttatg
     1141 aataacacca tgagtacaac cagggcaata atgaaaaggt gtatctgtta agccctttga
     1201 tttttcatat acaattgtca ttattttgta cctcctgcta ttttctcaat atctgccaag
     1261 atactgcccg gtgttggaac cataccgcct tgagtaccat tgaaatatac aggcagtctg
     1321 ccggcaatac ctatttttat gtcatccagt aattgtcccg tgctcatttc tacgccaagt
     1381 attccttttg cattaggacc aatctcttta aatacatcat acggataggg ccatatagta
     1441 atcggtctta tcataccaac ctttatgccc tttgcttgtg cctgcttaat tgcggatttt
     1501 actattcttg atgtagtacc gtatgctacg catacaacct ctgcatcttc taagccaaat
     1561 gcttcataac gaacttcgtt ttcttccatt attttatatt ttttttctaa tcttttattg
     1621 tgttcttgaa gttcttctgc ctgcaagtaa agtgagttta caacatttgg ttttcttttt
     1681 ccgccggttc ctgttgtagc ccaatcttta ggaggcaatt ctatcggttc cggttcctta
     1741 aattcaaccg cttccattat ttgaccaatc attccgtctg cagctattaa aacgggattt
     1801 ctgtaataat cagctttatc aaaagctaac ataacataat caactaattc ctgtatgctt
     1861 gaaggtgcat aacataatgt tctgtaatca ccgtttccgc cgcctcttgt agattgataa
     1921 taatctgctt gtgaaggctg tatgcttcca agtccggggc cgcctctcat catattaact
     1981 ataacacatg gaatctcagc tcctgcagcg taagtaatac cttcttgttt taaagatatt
     2041 ccggggcttg aagatgcagt catacatctt gctcccgctc ctcctgctcc ataaaccata
     2101 ttaattgcag ctatttcact ttctgcctgc agaaatactc cccctgcttc aggtaacttt
     2161 cttgaaatat attcagggat ttcacttgat ggtgttatgg gataaccgaa gaaacatttg
     2221 cagcccgcct taatggcagc agcacctaca gcttcattgc ctttcattaa tacttttgac
     2281 ataattaacc tccattataa tctttctact gttattaccg aatcgggaca cataattgca
     2341 cagttaccgc aagctataca tttttcttga tcagttacgt gtgcaggact gtaaccctta
     2401 ttatttattt tttccttatt taattcaata atttttaccg gacaaaccgc aacacaaagc
     2461 tcacaaccct tgcaagcatc gtcattaaat gtaaccttac cttttgccat tataaaacct
     2521 cctttaactc atccattctt ccctcatata aagatttatg gggaataatt tttctattat
     2581 ttcatggttc tttattttat cagctgtctc ttttataaca gcttcatatc ttatgggaat
     2641 atctgtttcc catgatacct tttttgtaag ctcatgaccg aattccacat cttctactgt
     2701 agttgcttta agcatgtgcg tattgttgac taatccggta atttttaacc ttgatgttgc
     2761 ttcaatcatt ttaagataat tgattactcc atcaacagtg cttgtatcag gtctgtttcc
     2821 gttaatcaca taaaacatat cataatcgac attttttatt tgttctgcga atctggcaag
     2881 aactcttgct cccacagggt cgcctccaac atcgattatg gatttaatat tcgggtttgt
     2941 tataactccc atcagttcag ccggcaatgc aggtaaatcg actgccgtgt ttctaatact
     3001 tgaatcgaat aattttatcc cgatttcttc aaaaaactct cttttttctc ttgatcggaa
     3061 ataaggatta actatatcta aatcggcaat actgacattc tcgtatattt ctttcatttt
     3121 aacggcataa ttaaccgcaa actctgtctt gccgctgccg tagtggccta taacaataac
     3181 taatcttttg tcatgcacaa tatccctcct ttcggaatat ttattatgtc ttaattatag
     3241 gttttagcct cctcaacacc ctttaaaact ctcaatccac cttcggccag agccaaaagc
     3301 tcgtcctctc ccggatagac tataacctta gctatgaatt ttactttatc ttcaatcatc
     3361 ttgacaaaat ccttgtcgta agcaataccg cctgttatta taatcgcatc aacatctccg
     3421 tcaagaacta ctgcatattc gccgattgcc tttgcaactt ggtatgccat tgcagaataa
     3481 atgagtcttg ctttatcatc tccgtcattt gctctgtcgc tgacttcttt tgcactgttt
     3541 gttcccaaat atgcacaaat tccgcccttg cctgctataa tcttatatat ttcagcttcg
     3601 gtatatttgc cgctaaagca catcttaact aattgcgcag caggtaatga gcctgttctt
     3661 tcgggtgaaa aaggtccttc gccgtttaga gcattgttta catcaattac tcttcccttt
     3721 ctatgtgctc cgacagaaat tccgcctcct aaatgaacaa ctacaaaatt cacttcatca
     3781 tactttttcc cgaacttttc aattgcggct tttcttgcaa cagccttttg atttaatgca
     3841 tgccaaatgc aaattctttc aaactccgga tgcccgctta tccttgccac atcctccatt
     3901 tcatcaacaa ccacagggtc tactataaat gatggaatat ttacttcatc ggctatggaa
     3961 tgcgccaaga gggctcccaa attggaagca tgctcacctt ttatattggt ttctaaatct
     4021 tttattaact tatcatttac caaataagtg ccgccttcaa tcggttttag taaacctcct
     4081 cttcctacta cagcgtccaa ttcatttaat gatatattgt tttcttttaa tgaggatatt
     4141 atcatttctt ttctgaattg aaactggtct acaaccctgt taaaaggctg caattcttcg
     4201 cttgtgtgtc gtatagtttt ttcaaaaatc atggtaacat catcaaatac tgctattttc
     4261 gtcgatgttg accccgggtt aataattaat tgtttcatat gctctccttt tttgaacaaa
     4321 ttactgctaa taaaattgaa taaagtttgg cttcatctga atcagccctt gaagtaagaa
     4381 tgattggagc cttagcacct aatataattc ctgcattttt tgaatcagct aaatatgtta
     4441 atgttttata cattacattt cctgcctcaa tattcggcat taataaaata tctgcatcac
     4501 ctgcaacttc tccctttact ccttttaaag ctgccgcttc cctggaaatt gcattgtcaa
     4561 gtccaaaagg tccctctacc atacagcctt ttatttcacc atttttgttc atattcacaa
     4621 gaatttcggc atcaacggta gcctgcattt taggtgatac tttttccttt gcacatatta
     4681 cagcaacttt tgggttttca atattcaatg acctgcataa gggaagggta ttttcgatta
     4741 tttgtttttt ttcagatgca gaaggtgcaa tattcattgc cgcatcagtt attatgaaca
     4801 gtttatggta tctgtccgat tcaaaaactg ctgcatgact taaaatattt cctgtcctaa
     4861 gtccgatttc cttatcgaga actgctttaa gaataattgc agtatcgact aatcctttca
     4921 tcaggatatc tgccttgccg ctgctgacca aggatactgc cgtcctagca gcttcattgt
     4981 cgtcttttga ctcgataatt tgaatatcat cgagattaaa gtttattttt tgtgatagtt
     5041 caattatttt ttctttattg cctatcaaaa ttggaatgca gattttttca agataagctg
     5101 attttacagc cattaacaca tcttcatcct gtgcggctgc aattgctatt ttttttggca
     5161 cagtggtttt tgccattttt aataactcat caaatctatt catatacaaa cacctcaaaa
     5221 tataacttgc aacaattatg ccaaaaaaac aaaaaaatcg accattgaaa tttcaacgat
     5281 agattttttt attaaatcag cttgaacttt tttgcatgca atttttttca agaaagttgc
     5341 attttgcaaa aaattgcaaa ttaaaaaatg ccgtatttct ccattttata atataaggac
     5401 cttaaagata tacccagctc tttggcagtt cgggttttat tcttgtcaaa tttcaaatat
     5461 acactttcaa tatatgcaga ttctgtttta ctcagtaccc tatccaaagt tgaaacatca
     5521 tcatcagtta atactgtact cttaaccgta ttttgaactt tatttgaatt cagctggggt
     5581 aaatgtgttg tcctgataac cctttcattt atattcatat atattatcgc ccggccgata
     5641 atattttcca attcccttac gttgcccggc cattcatgag acatcaaaac atctaaagca
     5701 tcatttgata tatcaacaat atttcttccg tattcatcat taaactttgc aataaaactt
     5761 tttaccaata aggggatatc ttcaatcctt tctctaagag gaggtatatt tatcggcaga
     5821 atgctgagtc tgtaaaataa gtcctctcta aattttcctt ccctcacgga ttttgccaaa
     5881 tctgcattag ttgctgcaat tacccttaca tctacaggaa tattcttgtt gcttccaaca
     5941 cgcattattt ctttttcctg cagaactctc aaaagttttg cctgagtatt taaactgatt
     6001 tcagaaatct catcaaggaa tatggtacct ttgtctgcct cttcgaaaag accctttttg
     6061 cctcccttta atgctccggt aaatgagcct tcatcatacc cgaaaagctc gctttcaaga
     6121 agcgaatcag tcagtgcggc acaatttacc cttacaaaat ttttgtgtga gcgggagctt
     6181 tgagcatgga ttgaattagc gaacaattcc ttgcccgtac cgctttcgcc tcttaagaga
     6241 acagttgccg gaaccttagc agccttttct atcagattcc ttacttgatt tattgctttt
     6301 gagttcccga taatatcttc aggtttatat ttataagtta gttctcttaa ctttttttca
     6361 gcttcctcta atttatcggt caagtccttt atagtagaaa tatcatgaag aacggcaaca
     6421 cttcccttca atttgccatt tacattaatt ggtgctgctt gagctacaac agtcttgccg
     6481 cttggtctta attttaattt tgtattttct acgggtttct ttgttgaaag cactttcata
     6541 tgcacgcttt cttctccgtc ttcaatatca tagagtgcat tcttgcctat tatatcgtca
     6601 tgctcaattc cggttatttt tgtataagcc ggattaacta agatatgctc gcctttttcg
     6661 tctacaacac ttattgcatc ctgtgttgca agaaaaaccg cctctaacat tccttggtaa
     6721 tccatgctgt taaaattaat tttttgcatc tttaatacct cttcctaagg ctctaaaggc
     6781 tcttttgaaa cttctattgt tacggtttct ttgcttaatg aaatttcttt gaaagtgaat
     6841 tcagaaatgc agtctaaatt aacagtgtta ataccattaa ctgtttctga taaatccagg
     6901 gttaaaatta aatcttcttt tttcagtgaa ttaactaagg ttgaagttcc ccgcacagta
     6961 accgttattt cttcttctgt ttcctttatc tctacatcac tgttttcaat tttatttata
     7021 aactgaatat catcatagct gtatcttaga tctctttcaa taattttttc taccacggca
     7081 tttacaaccg gtttagtttc catatccaaa aataccaaac cttctgcatt caaaatgttt
     7141 ctggttgaaa gtatattgtt gtatgcaccg gtaatatcca gttcttctaa ggtaagtttc
     7201 ttaaatgtat ctaatacatc ctgctctgcg gctatcctca ctctttcagg tttaacggtt
     7261 acatcgacca attcataacc ttcttccggt attcctgtaa ttgttggttc aagagctaca
     7321 tatttagtag gatatatcgg aactgtaacc tttacatttc ctattggagc cgataagaat
     7381 atttcattgt cagtatcatc atatatcctt accggaacag tcttaaccac atcatccatg
     7441 gcaccttcga tatttattgt agcaactgct ttatcagctg aatttacaac acttcttggt
     7501 cctgttatct ttactgatga cggatttgga actgtttgtg ctctgtaata gttcttagca
     7561 tgttctcctt caaattgaac cgtaacatcc ataaccttag aaattactcc ttctatgttg
     7621 catgctattt gcgaaggata tactcctacc acatcaacac cgcttggtcc tacaacttct
     7681 acctttgcat ttgttattcc ttcattaaaa cccaagacat ctatataggc agaaaaatcc
     7741 ctgctgctta ttttttccaa ttgctctgtt aatccggttg cttttactgt tacggttata
     7801 ttttccttgt cttgattcat tattactaaa ttagattttt ccagggcaga taagtttctt
     7861 attgttacag acacatttgg ataagactgt tcaagtgttt gattagttac aagcattaca
     7921 atcatccaca gaacaaatgc aacaataaga caggtaattt gaatgactaa tttatcgttt
     7981 tttatcttca tttctgtttc tccacttatc tataatattt accttttcat tttcaatata
     8041 aatactctgt aataatgatt caatttcttc attttgaata ttcctgtata gtcttccttc
     8101 cacagcatat gaaatgtacc ctgtttcttc agaaactatc aaagcaatgg aatctgaccg
     8161 ttcagtcatt cctattgccg ccctatgcct tgtccctata tctttactga gaaggttgtt
     8221 ttcggtaaga ggcaaaaaac atcctgctgc ttttattgta taatccttta taataactgc
     8281 tccgtcgtga aggggagtat taggaataaa aatatttatt aatagtccgc ttgaaatatt
     8341 ggcatttatt acagtcccgc tttctattat atcattgaga cctatctgtt tttcaaaaat
     8401 aattagtgca ccgatttttt gtcttgaaag agacgttatt gcagatataa tttctttaat
     8461 aatttttctg gaagtttcat tagtcttttc tacatttgac agggtaaaag aagttcttcc
     8521 caaatattca agagcttttc tgatttcagg ttgaaaaaca ataacaagcg cgattaatcc
     8581 gacttgaaat gtattgctta atataaattc aaccatatac aattcaaata tttgcgatat
     8641 aggaattaat gcaaccagta gtacaatacc cttaaccaat tgttttgctc tcatatctct
     8701 gattagaata taacctcgat aaagaacata gtaaacaata ccgatatcaa ctatggtatt
     8761 tggacctata tttgacataa ttaatcccat tcgatgcata atatcataca taataatccc
     8821 ccgcactatt cagcattcat ttgcgccatt gtatcggcat atgacctgct cattcttgct
     8881 ttagaatctt catccaattc tgcactttca atataatcca taatgggata aacaatatgc
     8941 tcggctgtac cgttttcttt aacaccttta taaacccata acggaatata attaatattt
     9001 ttaatggaaa tttcttttgt actgcccttt ttttctattt ctatatcaat aataattcca
     9061 tcttcggtat attttgaaac ttgctccgac ataccgtaag gaactaatgt ttccactctt
     9121 tgatttgata aaaaattacc catggaatat gctataaatt ttgttttacc gtcataatcc
     9181 aatgtttgcg taggttgtat aacatgagga tggcttccga gtattatgtc aactcctgac
     9241 ctgaaaagca tgtctgccaa aatttcctgc ctttgagctt gaattcttga atattcgtct
     9301 ccccaatgaa ggtatgctat tattacatcg gcattattat ctttggcata gattatgtct
     9361 tccttcattt tttcttcgtc tataatattt accattacat ccaagtcttc aggagtaaga
     9421 accgtttcaa gaccgtttac catttccgta tatgccataa atgcaaacct tatacccttt
     9481 acatccttaa ttaataccct tgtttcaggc tttgaagtaa aagttccgat tggttccatt
     9541 cctcttgctt taatttgctc tattgttctt ataatacctg cctttcttgt atcaaggctg
     9601 tggttgttgg cagttgacag tatgtcaaat cccgcatttt ttatagcgtc caatgcttca
     9661 tcgggagcat taaacaaggg atatccctga tatgcgtata cttcatttcc cgctgttgtt
     9721 ccttcaaaat tagcgattgc tatgtctgct tctcggataa tactttttat agctttaaaa
     9781 ctgtgttcaa aattataggt attcgttttg taatcatatg ctccatccaa ttgtgaggga
     9841 tgaaacatta tgtctcctgt cgctcttatt ctaacagtca catcaggttc aggctgaggc
     9901 tccttaactt cattagaggc ttcttctccg atttgttctt ccggatgctc ctccagcggc
     9961 tgcttgtctt ttttcccgtc tttacctata ccccttacaa caaaagaagc tgctaataca
    10021 ccaaccaata atactaatga ccatgacatt aatttaattc tttttttacg tcgtctgact
    10081 tttctgttca tatgctacct ctatgaataa ttacgtattt attctatcct actatgaaag
    10141 attttgcaag aaattaaatt attaaaatta aattaaagat accggatgtt ggtgccaggc
    10201 acccttaccc ggtgccaggc acctttagtg ctttttttgt cttggcttta ttaaagcttt
    10261 gggaccgta
//
LOCUS       DAOPUE010000066        10170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6659_length_10170_cov_10.592388, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000066 DAOPUE010000000
VERSION     DAOPUE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6659_length_10170_cov_10.592388"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1165)
                     /gene="spoIID"
                     /locus_tag="PKH42_06775"
     CDS             complement(131..1165)
                     /gene="spoIID"
                     /locus_tag="PKH42_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOA20173.1"
                     /translation="MLNRIIYAFVVFTMLLTIPNLSLNFFEKKMNLTSAAVQQKDSSK
                     YANEKHEEIYTADVYEPELIKVYNNKTNEVMIIDFEEYLKGVVASEMPAEFNIEALKA
                     QAVSARTYLLYRLKKYPDGQPEHSEAPVCTGVHCQVWNSKDELIRSHEEGWYDESWGK
                     IEEAVNSTRGQILVYDNKIIEPLYHSTSGGRTENSEDVFSTAVPYLRSVESPYEGDSP
                     RLNGSIKITTSEFIDKIESAYGDMDITESNLADKIVLGEVSEGGKIKTLIIDNTEISG
                     RDMRALFNLNSTNFSFIQSGNEIEILTTGYGHGVGMSQWGAEGMANRGYNYKEILKHY
                     YTGVEIVSMK"
     gene            1302..2021
                     /locus_tag="PKH42_06780"
     CDS             1302..2021
                     /locus_tag="PKH42_06780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOA20174.1"
                     /translation="MKNNRFARAKEKMKDFFIRLRHKDTSFLIIALCIMLLATAVVWR
                     YTSNPENGQSDLAENNEPNGDIQVHEDPYEDYVQDIIDEFQNAEDDNDEDDGVKPEGT
                     DLKSMKAPLAGEIIREFTLDELVYYEAIGEWRVHKGIDIKPKDTLLIESALAGKVEAV
                     NNSEITATEIIIDHGNNVKTLYSNLVSANVKAGDTVEKGQVIGNVGKTVSIEASDGAH
                     LHFEVIVDGQHINPLDYITIE"
     gene            2098..2373
                     /gene="spoIIID"
                     /locus_tag="PKH42_06785"
     CDS             2098..2373
                     /gene="spoIIID"
                     /locus_tag="PKH42_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOA20175.1"
                     /translation="MKDYIERRAMEIAEYIISTESTVRQTAKKFGVSKSTVHKDVTER
                     LPKLNPPAASEVKKVLLKNKSERHIRGGKATRLKYKEAQSQEKVSNM"
     gene            2556..3021
                     /locus_tag="PKH42_06790"
                     /pseudo
     CDS             2556..3021
                     /locus_tag="PKH42_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            3277..3438
                     /locus_tag="PKH42_06795"
     CDS             3277..3438
                     /locus_tag="PKH42_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20176.1"
                     /translation="MINLKRFLKYRDNAPEFKEIKDEGRVGLPCIVIENGEEILFDYN
                     LLDIESLKE"
     gene            complement(3467..4543)
                     /gene="buk"
                     /locus_tag="PKH42_06800"
     CDS             complement(3467..4543)
                     /gene="buk"
                     /locus_tag="PKH42_06800"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOA20177.1"
                     /translation="MKNSYKILAINPGSTSTKIAVYENEKLIFKETISHSNSDLEKFD
                     KITDQFKYRKDIILDTIKKHNFDLNELDAIVGRGGNFKPVEGGTYKVNKEMINDLIIG
                     VMGHHASNLGAIIADAIAKDLGLSAYIVDPVVVDELEDYARISGMPEIERKAKAHPLN
                     QKAVARLAAAELGGKYSDYNFVVAHMGGGISIGAHKNGRIVDINNCLDGEGTFTPERA
                     GGLPVGSLVEMCFSGKYTIQEIKKKITGKGGLVAYLGTNDAMEVKKRIAEGDKYAKLI
                     YETMAYQIAKDIGAAATVLKGKIDNIILTGGLAHDDVLVQWIKERVEFIAPVKVYPGE
                     FEMQAMVQGVIRVLTGEEELKIYR"
     gene            4776..>5859
                     /locus_tag="PKH42_06805"
     CDS             4776..>5859
                     /locus_tag="PKH42_06805"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOA20178.1"
                     /translation="MTASNDDAIRMLDEEIRYLRLLSKQYPTIASACTEIINLQAILN
                     LPKGTEHFLSDIHGEYESFNHVLRNGSGNIKRKINEVYGDIMSEKEIKSLATLIYYPE
                     QKLEIIRKEEEDIEDWYRITIYRLVVICRKAAFKYTRMKVRKALPKEFAYIIEELMHK
                     GPEEDKEEYYSEIINTIIRIGRADEFIIAMAKLIQRLVIDRLHIVGDIFDRGPGADKV
                     MDTIMNYHTVDIQWGNHDVLWMGAAAGSEACIATVLRICARYANLDIIEDGYGINVLP
                     LATFALEFYGDDECIQFKPKVESDMVYSDKDLKLIAKMHKAIAIIQFKLEGHIIKRRP
                     YFKMDDRLLLEKINYEEGTIDLYGKTY"
     assembly_gap    5860..5959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5960..6202
                     /locus_tag="PKH42_06810"
     CDS             <5960..6202
                     /locus_tag="PKH42_06810"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HOA20179.1"
                     /translation="GYTLIYNSYGLLLVSHDPFESTQKAIEEEKDIRSTTMVLEKELE
                     RKRVKDTDAGEVMKAQIKDLEMLLDAYRLGLIKEQG"
     gene            6292..7383
                     /locus_tag="PKH42_06815"
     CDS             6292..7383
                     /locus_tag="PKH42_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20180.1"
                     /translation="MKKFLSVFLTLALLLTLFPLTALASEPFSLEAPKNLTGELKIRD
                     DGIPYFELKLTVPESVKTINSNLVDNPDYYDGTSCDGIEIIFDYKYGNYDWNEGPSLY
                     WNTSMAVSDYLELGAYEYEPFDEGDFDTVDIKNEVYQFRARFNALWGYQGDWIDKDIY
                     SEYSNIVTIGNPAFYDDASDWAVAELEKANEAGLIPDILKGKDLTKSITREEFAELAL
                     LLYEKTTDKTVASVSENPFTDTTNPQIVKAFSIGITQGTSATTFSPNELINREQCATM
                     LFRTIKAIEPEGDFSIEGVKDFPDQADISSWAVEGTKFMAKLGIIKGDSNGNFMPKAV
                     TDAQKAVGYGMASREAAILMSVRTYEEYK"
     gene            7514..7852
                     /locus_tag="PKH42_06820"
     CDS             7514..7852
                     /locus_tag="PKH42_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HOA20181.1"
                     /translation="MSSFYSSIVHNFLIAFGVVIGASLFSGLGALMTGTPPLKVMTDV
                     AGSIKLWAIAASLGGTFSSFLVIEKGLFEGEIKTLLKQVFYIVISLTGANMGYEFIRL
                     LQRCGNIWGK"
     gene            7840..8361
                     /locus_tag="PKH42_06825"
     CDS             7840..8361
                     /locus_tag="PKH42_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20182.1"
                     /translation="MGEMMRVKGNVKNTAGYKYFICFITGLLWGTVIGSLGISILVSH
                     RMEELYEKIAYLEHTIQDKDEKLEKLESSINNANIVLKDISVILEFTGLSKEQIDLID
                     NIEIEKAIKEKYVSSLGKEIKSLDADILVQVIDKRILRFDDTEYQLTVNKLVLSDILK
                     LWVGVSLLEVTSP"
     gene            8477..>10170
                     /locus_tag="PKH42_06830"
     CDS             8477..>10170
                     /locus_tag="PKH42_06830"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOA20183.1"
                     /translation="MRIKYIKWPILILLLILIALVQYSAPDAYAEDLIKIVIDGKRIK
                     PDVDPYISNDRTLVPIRVIAEELDSVVEWDNDNRAVHISKEDIHILLRIDSYLVEYTN
                     DNETSYTIIDVTPQITGDRTFVPIRLISNALGVKIEWDNDKRTVYIDSSESSEVTKFF
                     DVEISSVKPGQTITGTTELYTKINNEIPKGAKEIKYLLLDRDTAKGFVIAGGSNINTP
                     YEWVPAMEDNGQKVLVSAFYDAQGNFLAGDSIPVTVKINPQIELKGIREGQLITAHSV
                     PLSTKLNFSASYVKYEMINPDNGAVYLSPGVDPEQSFTMIPVMEDNGNMSVRVIAYDT
                     KGNAYKGEYVNIGIDVDRYLYLGGVKHGQTIDGSVTLLAQRNFNVTDTEYIMVDNSTG
                     EETLLFKSGYGSYSWFPGPEDAGSKDLYVRVKDTAGNTHVSSRVTVNVIGTPKLMLQG
                     VGPGQVLTQAADLNIKTNVNLDSIKYILTNVRTGNEIIISEKSTGVIIPEKGDDGTWT
                     IRAQGTYGGRTIKSEEVKFTIYTGQLYSAKPVIEKDKYLDLVSGLAVNTRKTTGMSA"
BASE COUNT         3453 a         1626 c         1926 g         3065 t
ORIGIN      
        1 taccatacaa tgatatcaca caaagcagca attgtatata taattcagat ttttaagtca
       61 aacaatttgt cattgctatg aataacacat agtgtcattc tgaacgcagt gaagaatctt
      121 attattagtt ttatttcata ctcactattt caacacccgt atagtagtgt ttcaatattt
      181 ccttgtagtt atagcctcta ttagccatac cttcggcacc ccactggctc atccctacgc
      241 cgtgaccata cccggtagtt aaaatttcaa tttcatttcc cgattgtata aagctgaaat
      301 ttgttgaatt taagttaaat aatgccctca tatcacgccc cgaaatttcg gtgttatcaa
      361 ttattaacgt cttgatttta ccaccttcgc taacttcacc gagcacaatt ttatcagcca
      421 aattactttc tgttatatcc atatcaccgt aggcagattc aattttgtca atgaattcac
      481 tggttgtaat tttaatcgag ccgtttaatc tgggtgaatc tccttcatat gggctttcaa
      541 cgcttcttaa ataaggaact gccgtagaaa atacatcttc cgaattttct gttctgccac
      601 cgctagtgga atgatacagc ggctcaatta ttttgttatc ataaactaaa atttgtcccc
      661 ttgttgagtt taccgcttct tcaattttac cccagctttc gtcataccat ccttcttcat
      721 gagacctgat caattcatcc ttcgagttcc acacctggca atgaacacct gtgcaaacag
      781 gcgcttctga atgttccggc tgtccatccg gatatttttt taatcgatac aataaatatg
      841 ttcttgcaga aactgcctga gccttaagag cttcgatgtt gaattctgcc ggcatttctg
      901 acgcaactac accttttaag tattcttcaa aatcaataat catcacttca tttgttttgt
      961 tgttgtacac cttgattaat tcaggttcat atacatctgc agtgtatatt tcttcatgct
     1021 tttcatttgc atactttgag ctgtcctttt gttgcacggc agcgctggtt aaattcattt
     1081 ttttctcaaa gaaattcaaa gatagattag gaatagttaa aagcattgtg aacacaacaa
     1141 aagcataaat tatacggttt aacataaaaa ccaacccctt tatcataagt ttctttaata
     1201 ttctatgaaa aatagaaatt tttagaacaa aattgtatta aataaaaaaa atgggacaca
     1261 ctatttccag ataataaaga agtatcaaat gggaggttta tatgaaaaac aatagatttg
     1321 cgagagcaaa agaaaaaatg aaggatttct ttattagact aagacataag gatacaagct
     1381 tcttaataat tgctttgtgt atcatgctgt tggcaacagc agtagtatgg agatatactt
     1441 caaatcctga aaacggtcaa agtgatttag ctgaaaataa tgagccgaac ggagatattc
     1501 aggttcatga agatccttat gaagattatg tacaagatat cattgatgaa tttcaaaatg
     1561 cagaagatga caatgatgag gatgatggag ttaagccgga aggaacggat ttaaagtcca
     1621 tgaaggcacc attagcggga gaaataataa gagaatttac gcttgatgaa ttagtatatt
     1681 atgaggctat tggtgaatgg agagtacata agggaattga tatcaaacca aaagatacct
     1741 tattgattga atccgcatta gcagggaaag tagaagctgt aaataattca gaaattacag
     1801 caacggaaat tattattgat catggaaaca atgtaaaaac attgtacagt aatttagtgt
     1861 cggcaaatgt caaagccggt gataccgttg aaaaaggtca ggttattgga aatgtgggaa
     1921 aaacagtcag cattgaagca tcagatggtg cacatcttca ttttgaagta attgttgacg
     1981 ggcaacatat aaatcctttg gactatatta ccattgaata acaaaatcag gttctaatag
     2041 gacaaagaaa cgcataaaaa tgtaaataag acggtataat gccaaagggg gttaaaaatg
     2101 aaagattaca tagaacgcag ggccatggag attgcggaat atattataag tactgaatct
     2161 accgtaagac agacagctaa aaaattcggc gtcagcaaaa gcacagtcca caaggatgta
     2221 acagagcgtc ttcccaaact gaaccctcca gctgcaagcg aagtcaagaa agttttgctt
     2281 aaaaataagt ctgaaagaca cattagggga ggtaaagcta ctcgcttaaa atataaagaa
     2341 gcacagagcc aggaaaaagt cagcaatatg taattaacca cttgacgagg gacaccaacc
     2401 ggagggttgg tgtctttttt gttttccgac atgtaaccaa acaataaggc aatatgtaaa
     2461 aacatttgta attaatattt gatttaatct tttagaaata ttaaatcatt aaatgccaat
     2521 actacttaca gtttaacatg aaggagaata acactatgcc agagcttccg gaaatggaaa
     2581 catatcgaaa actacttgat aaaaagattt taggaaagac tattactgat gttgaaatta
     2641 acagggagaa gtcaattaat ataccaagtt tggaatttgt tcaggaagta aagggaaaac
     2701 agataacaaa gatagaacgc cgatgaaatt tgctttgaag caaagctcaa gccaactgtg
     2761 acaggagatt ctcttggtaa agatggtctg ttctcggttt ataattccat tcaaactgtt
     2821 ttaaagaatg ctatttctct tggcgggtat atggataatc ctttattttt aggggatcaa
     2881 catacagggg gatacaatgc tcatactctt gtttatggaa gagagggcaa gaaatgtagt
     2941 cgatgtggag gtactattgt taaaatagaa ctcaattctc gtaaatgctt ctattgccct
     3001 gattgccagc attcgtgtta agtttataat tgaagcattt attattgtat aattttatca
     3061 attgtcttat aatgataaat aatacaaaaa tagaaatttg ttagagaggt gttaattact
     3121 aataacattc tatttttatg gtaaatttaa tataaggaga agcgaatgaa gaaagtaatt
     3181 gtgtttggaa gcaaacattg accggattgt gaacctgcga aagagtttct ttcggaaaat
     3241 gaagttaatt acatatatct ggatatatca gaaaatatga ttaatcttaa acgatttttg
     3301 aaatacaggg acaatgcgcc tgaattcaag gaaattaaag atgaaggcag ggttgggtta
     3361 ccttgcatcg taatcgaaaa cggagaagaa atcctgtttg attataattt gttggatata
     3421 gagagtttaa aagaataatt aacccacatc atggtgggtt tttaaatcat ctgtatattt
     3481 ttaactcttc ctctccggtt aatactctta ttacaccttg taccattgcc tgcatttcaa
     3541 attctccggg atataccttt acaggcgcta taaattcaac tctttctttt atccattgaa
     3601 ccaggacatc atcatgtgct aatccgccgg tcaatattat attatcaatt ttccccttta
     3661 gaaccgttgc tgctgctccg atatctttag caatctgata tgccatagtt tcatatataa
     3721 gttttgcata tttatctcct tctgctattc tcttttttac ttccatagca tcatttgtgc
     3781 ctaagtatgc taccaagccg cctttgccgg ttattttttt ctttatttct tgaattgtgt
     3841 actttcccga aaagcacatt tcaactaatg agcctaccgg taatcctccc gctctttcag
     3901 gagtaaatgt tccttcgcca tccaaacaat tatttatatc tactattctt ccatttttgt
     3961 gtgctccaat cgaaatacct ccacccatgt gtgcaacaac aaaattataa tcggaatact
     4021 ttcctcctaa ttcggcggct gccagcctgg ctactgcctt ctgatttaaa ggatgagctt
     4081 tggctttcct ttctatttca ggcatccctg aaattctcgc ataatcttca agttcatcta
     4141 caactaccgg gtcaacgata tatgctgaca atcccaaatc tttggcaatt gcatcagcta
     4201 ttatggcacc cagatttgaa gcatggtgtc ccataacacc aattattaaa tcattaatca
     4261 tttctttatt aaccttgtaa gtgccgcctt ctacaggctt aaagttaccg cctctgccta
     4321 caattgcatc cagttcatta aggtcaaagt tatgtttttt tatcgtgtct aatattatat
     4381 cttttcgata tttaaactgg tcagtaattt tatcgaattt ctccaaatct gaattgctat
     4441 gagatattgt ttctttaaat attaatttct cattttcata tactgcaatt tttgtagagg
     4501 tagaccccgg attaatagct aaaattttat acgagttttt cataagtcct cccgatatag
     4561 gtacaactat attctttttc tttgattata tcaaaatttt tggaatttac aaaacctatt
     4621 atacaaattc tgttaagaag gttaaagtaa gccgcacaag aaaagaactc aagtttataa
     4681 taaaaattta aaaatttatg gcttttattc catttatgtt ataaactata tataggtaat
     4741 atccgttgtg gaaatataat aaaaggagaa atatcatgac tgcttctaat gatgatgcaa
     4801 ttcgtatgtt agatgaggaa attagatatt taaggctttt atcaaaacaa tacccgacaa
     4861 tagcttctgc atgtacagaa attataaact tacaagctat tttaaatcta cccaaaggta
     4921 cggagcattt tctatccgat atacatgggg aatacgaatc attcaatcat gttttaagaa
     4981 acggctcagg caatataaag cgtaaaatca acgaggttta cggggatata atgagtgaga
     5041 aggaaattaa aagccttgcc acattgattt attatcccga acagaaacta gaaataattc
     5101 gtaaggaaga agaagacatt gaagactggt atagaattac tatttacagg cttgtggtga
     5161 tttgcaggaa agcagctttc aagtatactc gcatgaaggt aagaaaagcc cttccgaaag
     5221 agtttgcata cataatcgaa gaactgatgc ataaagggcc tgaagaagat aaagaagaat
     5281 attacagcga aataataaat acgataataa gaataggcag agctgatgaa tttattattg
     5341 caatggcaaa gcttattcaa agattggtta tagacaggct tcatattgta ggagatattt
     5401 ttgacagagg tcccggtgcg gataaagtaa tggatactat tatgaattat catactgtgg
     5461 acatacaatg gggcaaccat gatgtattgt ggatgggtgc tgctgcggga agtgaagcat
     5521 gtattgctac agtattaaga atatgtgcca gatatgcaaa tttagacatc atagaagacg
     5581 gatacggcat aaatgttctt cctctggcaa catttgcact ggaattttac ggtgatgatg
     5641 agtgcattca gtttaaacct aaggtagaaa gtgatatggt ttattcagat aaggatttaa
     5701 aactaattgc aaaaatgcat aaagccattg ccattataca gtttaagctg gaaggtcaca
     5761 taataaaaag acgtccgtat tttaagatgg atgacaggct gcttcttgaa aaaataaatt
     5821 atgaagaagg aacaattgat ttatatggca agacatatcn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnng gatacactct catatataat tcctacggac tgctcttggt
     6001 atctcatgac ccctttgaat caacgcaaaa agccatagaa gaagaaaagg atattcgttc
     6061 gactaccatg gttttggaaa aagaattgga aagaaaaaga gtaaaggata ccgatgcggg
     6121 agaagttatg aaagcccaga taaaagattt ggaaatgctt ttagatgcat acagattggg
     6181 tttaataaaa gaacagggat aattaatagg aaaaatcacc ctcagggtga ttgaaataat
     6241 tgttgattcc aatataattg atataaatat tataattata agggggttac aatgaaaaaa
     6301 tttttatctg tctttttgac tctggcactt ttattgacat tatttccatt gacagcattg
     6361 gcaagcgagc ctttttcttt ggaagcacca aaaaatctta caggcgaatt aaaaattagg
     6421 gatgacggta ttccttattt tgaattaaag cttaccgttc ccgaaagtgt aaaaacaatt
     6481 aattctaatt tggtagataa tcctgattat tatgacggta caagttgcga tgggattgaa
     6541 attatttttg attacaaata tggcaattac gattggaatg aaggtccctc attatactgg
     6601 aatacttcaa tggctgtttc tgattatctt gaacttggtg cttatgaata tgaaccattt
     6661 gatgaaggag attttgatac cgttgatatc aaaaacgaag tttatcagtt ccgtgctcga
     6721 ttcaatgcac tttggggcta tcagggcgat tggattgaca aagatattta ttcagagtac
     6781 tctaacatag ttacaatagg taacccggca ttttatgacg atgccagcga ttgggcagta
     6841 gctgaattgg aaaaagccaa tgaagcagga cttattcctg atatattgaa gggaaaagat
     6901 ttgacaaaat caataacccg tgaagaattt gcagagctgg ctctcttgct ttacgaaaaa
     6961 actactgaca agacagtggc aagcgtatct gaaaatccat ttaccgatac gaccaatccc
     7021 cagattgtaa aagcattttc tataggaatt actcaaggaa catcagcaac tacattttca
     7081 ccaaatgaat taattaatcg agaacaatgc gcaaccatgc tttttagaac aataaaagca
     7141 atagagcctg aaggtgactt tagtattgaa ggtgttaagg actttcctga ccaagcagat
     7201 atttcatcat gggcagtgga aggaaccaaa tttatggcta aactgggaat aataaaaggt
     7261 gacagtaacg gtaattttat gcctaaggct gtgacggatg ctcaaaaagc tgtcggttac
     7321 ggcatggcat caagagaagc tgcaattctt atgtctgtca gaacatacga agaatataaa
     7381 taaatacact gttattgtaa tgaattcaat ttgaaggctc agacctcgta actctgaact
     7441 cattgaagaa actaaacaat caatcataaa gacatttttc actcatatta taaaaagaga
     7501 gtatggtgat aaaatgtctt ctttttattc aagtatagtg cataactttt taattgcatt
     7561 tggagtcgtt ataggagcaa gcttgttttc cgggttggga gctcttatga caggcacacc
     7621 ccctttaaag gtgatgacag atgttgcagg ttcaatcaaa ttatgggcta tagcggcatc
     7681 cctgggtggg accttttcat ctttcttggt aattgaaaaa gggctgtttg aaggagaaat
     7741 aaaaacattg ttaaaacaag tattttatat agtgatttca ttaaccggcg caaatatggg
     7801 ttatgaattt atcaggcttc ttcaaagatg cggcaatata tgggggaaat gatgagggtt
     7861 aaaggaaatg taaaaaacac tgcaggatat aaatatttta tttgctttat aacgggactg
     7921 ctttggggaa cagttatagg ctctttgggt ataagtatat tagtcagcca tagaatggaa
     7981 gagctttatg aaaagatagc atatttggag cataccattc aagacaaaga tgagaaactg
     8041 gaaaagcttg aaagttcaat caacaatgca aatatagtgc tgaaggatat ttccgtcata
     8101 ttggaattta ccggtttaag caaggaacaa atagacctga tagacaatat tgaaattgaa
     8161 aaagcaatta aagaaaaata tgtttcatcc ttgggcaagg aaatcaaaag tttggatgca
     8221 gatatacttg tgcaggttat agataaaaga atattaagat ttgacgatac agaatatcaa
     8281 ctaaccgtta ataaacttgt gctgtctgat attttaaaat tgtgggtagg agtgtctctg
     8341 ttggaagtaa cttcgcccta acaatatttt tttgacagaa gggacaaact ctataggaga
     8401 tattgtaata taatgttaat gtttattatg tcggttttca tggtataatg taaaaaaaga
     8461 aataaagagg tgggcgatga gaattaaata tattaaatgg ccaattctta ttctgttgtt
     8521 aatattgatt gctttggttc aatattcggc acccgacgct tatgctgaag atttaattaa
     8581 aatagtaata gatggcaaga gaataaaacc cgatgtggat ccttatataa gcaatgacag
     8641 gacattggtg cccataaggg ttattgctga agaattggat tctgtcgttg aatgggataa
     8701 tgataacagg gcagtccata tatctaaaga agatatacac atacttctta gaattgacag
     8761 ctacttagtt gaatatacaa atgacaatga aacttcttat acaattattg atgtgactcc
     8821 tcaaataacc ggagaccgta cctttgttcc catacgttta ataagcaatg ccttgggtgt
     8881 gaagatagaa tgggacaatg ataaaagaac ggtatatata gattcatcgg aaagctcaga
     8941 agttacgaag ttttttgatg tggaaatttc atcggtaaaa cctggacaaa ccattaccgg
     9001 aacgactgag ctgtatacaa aaattaataa tgaaataccg aaaggagcaa aagaaattaa
     9061 atatcttctt ttggatagag atacggcaaa aggatttgtt attgcaggag gcagcaacat
     9121 aaatacccct tatgaatggg ttcctgcaat ggaagacaat gggcagaagg tacttgtttc
     9181 cgcattttat gatgctcagg gaaacttctt ggcaggtgat tcgatacctg ttacggtaaa
     9241 aataaatcct cagatagagc tgaaaggaat aagggaaggt caattaatta cagctcattc
     9301 ggtaccttta agcacaaagt taaatttctc ggcatcatat gtaaaatacg aaatgataaa
     9361 tcctgataac ggagctgtct atctgtctcc cggagttgac ccggagcaat catttactat
     9421 gattcccgtt atggaagaca acggcaatat gtctgtaagg gttattgcct atgatactaa
     9481 gggaaatgca tataagggcg aatatgtaaa cattggaatt gacgttgaca ggtatcttta
     9541 tttaggcgga gttaaacatg gacaaactat tgacggctct gtaactttac tggcacaaag
     9601 aaatttcaat gtaaccgata ccgaatatat aatggttgac aattcaaccg gtgaagaaac
     9661 tttgttgttc aaatcgggtt atggttcata ttcctggttc ccgggacccg aagatgcagg
     9721 aagcaaggat ttgtatgtaa gggtaaaaga tacggcaggc aacactcacg taagcagcag
     9781 agtaacggtt aatgtaatcg gcacaccaaa gcttatgctt caaggcgttg gacccggaca
     9841 agtattaact caagctgcag acctaaatat aaaaactaat gtaaacttag acagtatcaa
     9901 atatattctt acaaatgtaa ggacaggtaa tgaaattatc atctcggaga aaagcaccgg
     9961 agtaattatt cccgaaaaag gtgatgacgg aacatggacc ataagagccc aagggacata
    10021 cggcggtagg acaataaaat ccgaagaggt taaatttact atttatacag gtcagttata
    10081 ttcagcaaaa cctgtaatag aaaaagacaa atatttggac ttggtttccg gtttagccgt
    10141 aaatacaaga aaaaccacag gcatgtccgc
//
LOCUS       DAOPUE010000067         9840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_6936_length_9840_cov_8.439448, whole genome shotgun sequence.
ACCESSION   DAOPUE010000067 DAOPUE010000000
VERSION     DAOPUE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_9840_cov_8.439448"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1647)
                     /gene="murJ"
                     /locus_tag="PKH42_06835"
     CDS             complement(100..1647)
                     /gene="murJ"
                     /locus_tag="PKH42_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOA20184.1"
                     /translation="MSNVRKTALIITALTLGSKFLGFMREIALAYFYGTSYVIDAYVM
                     AVAIPGIVFGWIASLAVSYTPIYMDAKVKLGANKSIRFTDNMISIGITISIFCVLIGV
                     IFSSKLVSIIAPGFEGDVYELTDRFVKIYLFSIVINVFAQVLVSFLNCNSKFILSSIS
                     VFVVNGIQIVFIFISSRIGKDALIYGAVLSNIAQLIFLYVFSGRNGYRLRYEFKVTPE
                     IKQAFIMLIPIFLSSMVSQLNGFVNKVFASGLIEGSVSALNYSMSVKTFIQSVFSVAI
                     TTMIYPMLSQSIAENDINAVKKMVSNAVNIIIILFIPITVGVVLLSEPAIFLVYERGM
                     FSSESTAMTSIALQMYSLSLVAVCLIDVLTKMFYAMKDAKTILYLNTFTVAICIASSI
                     ILVKPMMHAGLALAGSLAEIITLPLFFFFLKRRIGNLGMKNSFNILVKSCICSAIMGI
                     TVCFVYNYSYYALSTDKLYILLSILISVAAGTLTYFVLMNLMKVKEMDFFTYIIKDIF
                     RKAFGKI"
     gene            complement(1706..3034)
                     /locus_tag="PKH42_06840"
     CDS             complement(1706..3034)
                     /locus_tag="PKH42_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008777765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOA20185.1"
                     /translation="MNIILLSGGSGKRLWPLSNETRSKQFLKLLKSSDGKIESMIQRV
                     YRQIKEASIDADIVVATGKYQLDLIKSQIGNNVDIVLEPERRDTFPAIALSCAYLAYV
                     KNMSMDEVVLVLPVDPYTESDYFMALIKMEKAVLNGKAKMALMGIKPTYPSDKYGYII
                     PGKTGDFIEGFLEKPSEEYASKLIGDGAMWNGGVFAFKLGYLIDIIKKYIDFNSFKEI
                     ERQYADLPMTSFDYEVVEKEKSITMIEYRGAWRDLGTWNTLTEVMDCNSLGNVILADS
                     CCNTHVINELDIPVAVLGAYNMVVAASPDGILISDKKESSCLKAYVENIRQRPMYEER
                     RWGEYKVLDNVVYQDGMSALTKSLTIKAGKSISYQSHAIRDEIWTIVDGTGEVITDGH
                     IRNIKRGDVVYISKGQKHAIRAVSDLSVIEVQIGNELVESDIERYNCNLL"
     gene            complement(3170..4159)
                     /gene="galE"
                     /locus_tag="PKH42_06845"
     CDS             complement(3170..4159)
                     /gene="galE"
                     /locus_tag="PKH42_06845"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017470950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOA20186.1"
                     /translation="MNILVTGGAGYIGSHTCVELLNNNHSVIIADNLCNSKIETINKI
                     KQITNKDVLFYQVDVTDRQAVESIFSSYQIDGVIHFAGLKAVGESVEKPLEYYYNNIV
                     STIILSKACIKYNVNKFVFSSSATVYGENKVPFIETMDLLPTTNPYGETKAISERILM
                     DVAKVNPNFSVSLLRYFNPVGAHKSGLIGESPIGIPNNLMPYVTQVAKGKLEKLRVFG
                     NDYNTVDGTGVRDYIHVMDLAEGHVAAIEKLTEGVHIYNLGTGQGTSVLQLIHAFEEV
                     NNIKVPYEIVGRRPGDIASCYADASKAERELGWKAKRGIKEMCRDAWRFEKGL"
     gene            complement(4351..5421)
                     /gene="rfbB"
                     /locus_tag="PKH42_06850"
     CDS             complement(4351..5421)
                     /gene="rfbB"
                     /locus_tag="PKH42_06850"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOA20187.1"
                     /translation="MKTILVTGGAGFIGSNFVKFMLKKHPDYRIINIDALTYAGNLEN
                     FKEIENNPNYKFIKCDIRDRKKIDKIFDSYDIDAVVNFAAESHVDRSIEEPEVFLTTN
                     IIGTQVLLDVAKKFWKINPDDKYCKGYKPGVKFIQISTDEVYGALGKTGKFVESMPLM
                     PNSPYSASKASADMIVRAYHKTYGMPVNITRCSNNYGPYQFPEKLIPLMINNCLKEIE
                     LPIYGDGMQIRDWLHVTDHCSAIDMVLHKGLDGEVYNIGGNNEKANIEIVKIIIQTLG
                     KSENLIKYVKDRPGHDRRYAIDNTKITTELGWKPAYTFEQGIKETIQWYLDNAEWIEN
                     IVSGDYVNYYEKMYSGVGKESK"
     gene            complement(5446..6315)
                     /gene="rfbA"
                     /locus_tag="PKH42_06855"
     CDS             complement(5446..6315)
                     /gene="rfbA"
                     /locus_tag="PKH42_06855"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOA20188.1"
                     /translation="MKGIILAGGKGTRLYPMTQAISKQLLPIYDKPLIYYPLSVLMLA
                     GIREILIISTPEDTPIYERLLGDGSTLGVRFTYKVQERPRGLADAFIIGEDFIGNDNV
                     CLILGDNIFYGQDLTRVLNKASAIKEGAVIFGYPVKDARAFGVVEFDENKKVISIEEK
                     PKYPKSNYAVPGLYFYDSRVVDIAKNVKPSERGEIEITSVNNAYLENKELNVILLGRG
                     MAWLDTGTPEGMLKAAEYVEAVQSRQGFYIACLEEIAWRRGFINNEQLKTVGESLKIT
                     DYGQYILSLLDEV"
     gene            complement(6325..7419)
                     /locus_tag="PKH42_06860"
     CDS             complement(6325..7419)
                     /locus_tag="PKH42_06860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOA20189.1"
                     /translation="MNKSIQVTRSSIPDIEDYIKEIKDIWDTRLLTNMGAKHRVLEAQ
                     LVDYLNVPNITLFTNGHLALECIIAALDLKGEVITTPFTFASTTHAIVRNGLKPIYCD
                     INSEDYTLDVYKIESLITEKTSAIIPVHVYGNICDVKAIDAIAKKHNLKVIYDAAHTF
                     GVTVNGQGIGTFGDASMFSFHATKVFNTIEGGAVTYNEPSLKGRLDSLKNFGITGPDT
                     VEYVGGNAKMNEFQAAMGICNLRHVDKEIAKRKIVVERYNEQLGDTDGIKLSKPQAGV
                     KSNYAYFPVVFDDYKKSRDEVFNELKTHNIFARKYFYPLTNSFDCYKGNYNVEDTPIA
                     KYISERVLTLPLYADLDLEDVDRICEIIKK"
     gene            complement(7412..7888)
                     /locus_tag="PKH42_06865"
     CDS             complement(7412..7888)
                     /locus_tag="PKH42_06865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOA20190.1"
                     /translation="MDVQIRIKAYQKFFNIEENPIIEHDVWITRTHGLEGKIKIGNNV
                     TLAKNVFIDYSGNVIIEDGVLLASGVKIESHYRDIDAYLAGKNINIPSSVLIREKAFI
                     GTNAIILPPCKYIGKNSRIGAGAVVVSDVPDNAVVVGVPAKIVKYINFSEDEVNNE"
     gene            complement(7971..>9840)
                     /locus_tag="PKH42_06870"
     CDS             complement(7971..>9840)
                     /locus_tag="PKH42_06870"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOA20191.1"
                     /translation="ECDVMDYVPIIYSKDYIPELIDKCYKHKIDLIIPGSDDEALILA
                     EHKSMLEENGFKVLVSDEKLISLIRDKAKMCDELSSISDVFVKNYDFEEIKLLIKNNE
                     ISFPLIAKPRDGYASKGIEILLEEKDLVRVTNQHIIQELAIPHKGDPFRQAYLQQISK
                     KVNSQLSEISIQIVTNKNGDLMGRMASYNKLNNGVPIEIIPYDNEYIWSEIDKLIPML
                     KQLGHRGPLNIQGRLTDKGLKLFEMNARFTGITGLRAIMGFNEVECCIKDWLNLSNKN
                     ETLKLNYGRFGIRQTTDKIISLNKNKRIKELSLSINKKELKTGKIILVTGASGYLGQT
                     LIKKLLKSKYEVWALSRNKFKIEKLYNHFEGVKCFEYDDIKNGSLSIGLVDTIIHCGF
                     ARPHKGNEEITDSLNFTNQLFNLAAIHQIPEIINISSQSVYGTKQAIYWTEKTKVMPE
                     TIYAQAKYCTELMAENIKLISKHSNVTSLRLSSLAGGQEGLVPVDIISKFVINVINNE
                     TIEVIDGKQKLQRLDIRDAADGIIALLKTNSDEWETVYNLGMEGSYTVLEMADMATKI
                     GKEMLGSNVIVNVKAEKIDLELGMDSNRFYSLTKWKPRYSLKDIVESLFKYYRKER"
BASE COUNT         3065 a         2038 c         1254 g         3483 t
ORIGIN      
        1 ttttgctcat taccttttcc gcccccttta ttttaatgta ttaacgtaat tcaattttac
       61 tttttaatca ttatccccct tgcttgctgt ataaaatcgt taaatctttc cgaatgcctt
      121 ccgaaatatg tctttaatta tataagtaaa aaagtccatc tccttaacct tcataagatt
      181 cattaaaaca aagtatgtca atgtccctgc agcaactgaa attaaaatcg ataaaagtat
      241 ataaagcttg tctgtgctta gtgcataata tgaataatta tatacaaaac atactgttat
      301 acccattatt gcagagcata tacatgattt aacaagtata ttaaaactat ttttcattcc
      361 aagatttccg atgcgcctct ttaaaaagaa gaaaaacaaa ggaagtgtta taatctccgc
      421 caagctgcct gcaagagcaa gccctgcatg catcattggt ttaaccagga ttatacttga
      481 tgcaatgcat attgcaaccg tgaatgtatt caaatacaat atcgttttcg catccttcat
      541 ggcataaaac atcttagtta aaacatctat caggcaaaca gcaaccaggc ttagagaata
      601 catctgtaaa gcaatagagg tcatggctgt cgattcactg ctgaacattc ccctctcata
      661 cacaagaaat attgccggtt ccgacagtaa aacaacacct acggttatcg gaataaataa
      721 tattattatg atatttacag catttgagac cattttttta acagcattaa tatcattttc
      781 tgcaatgctt tgtgaaagca tggggtaaat catagtagta attgcaacag aaaaaacact
      841 ttgaatgaat gtcttgacag acatggaata attaagtgcc gaaacactgc cctcaattag
      901 tccggatgca aataccttgt ttacaaaacc atttaattga ctgaccatgc tgctcaaaaa
      961 tataggaatc agcataataa atgcttgctt aatttcaggt gtaaccttaa actcgtatct
     1021 taatctatat ccgtttctcc cggaaaacac ataaagaaat atcagctgtg ctatatttga
     1081 taaaaccgca ccatatatca acgcatcctt accaatcctg ctgctgataa aaataaacac
     1141 aatttgaatg ccatttacga caaagaccga tatactggat aatataaatt tactattaca
     1201 attcaaaaaa gatactaaaa cctgggcaaa aacatttata actattgaaa ataaatatat
     1261 cttcacgaac ctgtcagtaa gttcataaac atctccttca aaacccggag caataatact
     1321 gactagctta ctgctaaaaa tcacgcctat aagtacacag aaaatggata tagtaatgcc
     1381 aattgatatc atattatcag taaaacgaat tgacttattt gccccaagct ttaccttagc
     1441 atccatatat ataggagtat atgataccgc caaagatgca atccatccaa aaactatacc
     1501 cggtattgct acagccataa cataggcatc aatcacatat gatgtcccat aaaaatatgc
     1561 taatgcaatc tcacgcataa atcctaaaaa ttttgagccc aaggttaatg cagttattat
     1621 taatgctgtc ttccttacat ttgacatatt attcctcagc tatcatttgt gataattatt
     1681 attaagcctt tcgtcggaaa tttttttata acaaattaca gttatatctt tcaatatcac
     1741 tttcaaccaa ttcatttcct atttgcactt ctatcacact taagtccgaa acagccctta
     1801 tcgcatgctt ctgacctttc gaaatataaa caacatcgcc tctttttatg tttctgatat
     1861 gcccatctgt aattacttcc cctgtcccgt caactattgt ccaaatttca tctcttattg
     1921 catgagattg atagcttatc gacttaccag ccttaatagt caagcttttt gtcaaagccg
     1981 acataccatc ttgataaaca acattatcaa gcactttata ttctccccag cgcctttcct
     2041 catacatcgg acgctgacgt atgttttcta cataagcttt taaacatgaa ctttcttttt
     2101 tgtcagaaat caggatgcca tcgggactag ctgctactac catattatat gctcccaaca
     2161 ctgctaccgg gatatccaat tcatttatta catgggtgtt gcagcagctg tcggcaagta
     2221 ttacatttcc taaagaattg caatccatta cttcggttaa tgtattccac gttcctaaat
     2281 ctctccatgc ccctctatat tctatcattg taattgactt ttccttttca actacctcat
     2341 aatcaaaact tgtcataggc aagtctgcat attgtctctc tatttcttta aaagaattga
     2401 aatcaatgta tttcttaata atgtcaataa ggtatcccaa tttaaaagca aagacgcctc
     2461 cattccacat tgcgccatcg cctattaatt ttgaggcata ttcttccgaa ggtttttcca
     2521 gaaatccctc aataaaatct cctgtcttac cgggtattat atagccatat ttatctgaag
     2581 gatatgtagg cttaatcccc attaatgcca tttttgcctt accattaagt actgccttct
     2641 ccattttaat taatgccatg aaataatccg attcagtata cggatcaaca ggcagaacaa
     2701 gaactacttc atccatgctc atatttttta catatgcaag ataagcacac gacaacgcta
     2761 ttgccgggaa tgtatctctc ctttcaggct caagtacaat atctacattg tttcctatct
     2821 ggctttttat taaatctaat tgatatttac ccgttgcaac aactatatct gcatctattg
     2881 atgcttcctt aatctgtctg tatactcttt gaatcattga ttcgatttta ccgtcactac
     2941 tcttcagcaa tttcaaaaat tgttttgatc tcgtttcatt tgaaagcggc cacaatctct
     3001 ttcctgaccc gcctgacaaa agtattatat tcatatacta atctcctaaa cctgcaattt
     3061 ttctatttct ttatcccctc ggatttcatg cttataaata gaccattgtt tagtaaatgg
     3121 cggtgtatat tgtgtataac ctaaactgat taacacttgt cattatttat tacaacccct
     3181 tctcaaaccg ccaagcatcc ctacacattt cctttatccc acgctttgcc ttccaaccta
     3241 gttctcgctc tgctttacta gcatccgcat aacaagaagc aatatctccg ggtctgcgac
     3301 cgacaatttc ataaggtact ttgatattat taacttcctc gaaagcatgt attagttgaa
     3361 gaaccgaagt tccttgtcct gttccaagat tgtatatatg cacaccttca gttaactttt
     3421 caattgctgc aacatgacct tcggcaaggt ccatcacgtg aatatagtct ctaacccctg
     3481 taccgtctac agtattataa tcattaccaa atactcttaa tttctcgagc tttccttttg
     3541 ctacctgagt tacgtaaggc attaagttgt ttggaatacc aataggggat tcaccaatta
     3601 atccgctttt atgtgctcct accggattaa aataacggag aagcgaaacc gaaaaattcg
     3661 gattaacttt tgcaacatcc attaatatcc tctcgcttat tgccttggtt tcgccataag
     3721 gattagttgt tggaagtaag tccatagttt ctataaaagg aactttattc tcaccatata
     3781 ctgtggctga tgaactgaat acaaatttat tcacattata tttaatacaa gctttagaaa
     3841 gtataattgt actaacaata ttgttatagt aatactcaag aggtttttca acagattcac
     3901 ctacggcttt tagacctgca aaatgaatta caccgtcaat ttgataggat gaaaatatag
     3961 attccactgc ttgtctatca gttacatcta cttgatagaa taaaacgtcc ttattagtta
     4021 tttgctttat tttatttata gtctcaattt tgctgttaca taggttatct gctattatta
     4081 cagaatgatt attatttaat aattcaacac aggtgtgtga acctatatag cctgctccgc
     4141 cggtaacaag tatattcatt taacttacct caacaatttc ttataatatt gctttattat
     4201 atatatagat ttaatcttac tttataaact ccacatacca ctcagcaaac ttacttaacc
     4261 cctctttaat agaagtctcc ggttaaaacc taccgcttca tgcaataatc tgtagaagca
     4321 taggtaacag gatcatcccc taccatagct tcatttagat tctttgccaa cgccagaata
     4381 catcttctca taataattca cataatcacc tgatacaata ttttcaatcc actcagcatt
     4441 atccaaatac cactgaatag tctctttaat accctgttca aatgtgtacg ctggtttcca
     4501 tccaagctct gtggttattt tagtattgtc gatagcatat ctcctgtcat gacccggacg
     4561 atctttcaca tactttataa ggttttcaga tttaccaaga gtctgaataa ttatctttac
     4621 aatctctata ttagctttct cgttattccc gccaatattg taaacttcac catctaatcc
     4681 tttgtgaaga accatatcaa ttgctgagca gtggtcagta acatgcagcc aatctcttat
     4741 ttgcattcca tccccataaa tcggaagctc tatttcctta aggcaattat taatcatcag
     4801 aggtattagc ttctcaggga actgatatgg tccatagtta ttgctgcaac gtgttatatt
     4861 aaccggcatt ccataagttt tatggtaagc tcttacaatc atatctgcac ttgcctttga
     4921 tgctgagtat ggactatttg gcataagggg catagactca acgaacttac ctgtcttccc
     4981 taatgcaccg tatacttcat ctgttgaaat ctgaataaat ttaacccccg gcttatatcc
     5041 tttgcaatat ttgtcatctg gattgatttt ccaaaacttc ttggctacat ccaacaaaac
     5101 ttgtgttcct ataatatttg ttgttaaaaa cacttccggt tcttcaatac tcctgtcaac
     5161 atgtgattca gctgcaaagt taactacagc atcaatatcg tatgaatcaa aaattttatc
     5221 tatctttttt ctgtctctta tgtcgcattt aataaattta taattaggat tattctcaat
     5281 ctctttaaaa ttttctaaat tccctgcata ggtaagagca tcaatattaa taattctata
     5341 gtcagggtgc tttttaagca taaatttcac aaaattgctt ccaataaacc cagctccacc
     5401 tgttaccagt atagttttca aattgtttta cctcacttcc atgaatcata cttcatctaa
     5461 taatgacagt atatattgac cataatcagt aatctttaaa ctttcgccaa ccgttttaag
     5521 ctgttcgtta ttaataaatc ctcttctcca tgcaatctct tcaaggcatg caatataaaa
     5581 gccttgcctt gattgaactg cttctacata ttctgcagcc ttaagcattc cttccggtgt
     5641 acctgtatcc aaccatgcca ttcccctacc taatagaata acatttaatt ctttattttc
     5701 taaatatgca ttattcacag aagtaatctc tatttctccg cgctcggacg gcttcacatt
     5761 tttagctata tctacaacac ggctatcata aaaatatagt cctggcactg catagtttga
     5821 ttttggatat tttggctttt cttctataga tataaccttt ttattctcat caaactctac
     5881 aactccaaat gctcttgcat ctttaacagg ataaccaaaa ataactgctc cttcttttat
     5941 agcagatgcc ttatttagta ctcttgtcaa atcctgacca tagaaaatat tgtctcccaa
     6001 tataagacag acattatcat tgccaataaa gtcttctcca ataataaatg catcagctaa
     6061 tcctcttggt ctttcttgaa ccttataggt aaatctaact ccaagagtcg aaccatcccc
     6121 tagcagtctc tcgtaaatag gcgtatcctc aggtgttgaa attattaaga tttccctaat
     6181 tccagctaac atcaatacag atagaggata atatatcaat ggtttatcat atataggcaa
     6241 taattgcttt gaaattgcct gagtcatggg ataaagtctt gtcccttttc cacctgcaag
     6301 tataattcct ttcatctgct cctcctattt cttaataatt tcacagattc tatctacatc
     6361 ttctaaatct aaatctgcat ataacggcag tgtgagaact ctttcagaaa tgtattttgc
     6421 tatcggtgta tcctcaacat tataattgcc tttataacaa tcaaagctat ttgtaagagg
     6481 ataaaaatat tttcgtgcaa aaatattatg cgtttttaac tcattaaata cttcatctct
     6541 acttttttta tagtcatcaa atacaacagg aaaatatgca tagttactct taactcctgc
     6601 ttgaggtttc gataacttaa taccatcagt atcacctaat tgctcattat atctttcaac
     6661 tacaattttt cttttagcta tttctttatc tacatgtctt aggttgcaaa tacccatagc
     6721 tgcttgaaac tcattcattt ttgcattacc tccaacatat tctacagtat ctggtccagt
     6781 tattccaaag ttcttaagtg aatcaagcct tccttttaaa gaaggttcat tatatgtaac
     6841 agctcctcct tcaatagtgt taaaaacttt tgtagcatga aaactaaaca ttgaagcatc
     6901 tccaaaagtt cctattccct gtccattaac tgttacacca aaagtatgag cagcatcata
     6961 aataacttta agattatgtt ttttagctat agcatcaatt gctttaacat cacatatgtt
     7021 tccataaaca tgaacaggta taatcgcaga agttttctct gtaatcaaac tttcaatttt
     7081 ataaacatct aaagtgtaat cttcagaatt tatgtcgcaa taaattggct tcaacccatt
     7141 acgaacaata gcatgcgttg tggatgcaaa agtaaatggt gtagttatta cttcaccctt
     7201 taaatctaat gcagctatta tgcattcaag tgctagatga ccatttgtga ataaggtgat
     7261 atttggtaca ttaagataat ctacaagctg agcttcaaga accctgtgct tagcgcccat
     7321 atttgtaagc aatctggtat cccatatatc tttaatttct ttaatataat cttctatatc
     7381 tggtatagag gaacgtgtta cttgtattga tttattcatt atttacctca tcttcactaa
     7441 aattgatata ctttactatt ttagccggta ctcctactac cactgcatta tccgggacat
     7501 cacttacgac aaccgcacct gcaccaatac gtgaattttt accgatatat ttacatggag
     7561 gtaaaattat tgcattagtt cctataaatg ctttttctcg tattaataca cttgatggta
     7621 tattaatatt cttgcctgcc aaataggcat ctatatccct ataatgagat tcaatcttta
     7681 cgcctgaagc taataatacg ccatcttcaa taataacatt gcctgaataa tcaataaata
     7741 cattttttgc taatgttacg ttattcccaa tttttatctt cccttctaaa ccatgtgtcc
     7801 tggttatcca tacatcatgt tctataatcg gattttcctc gatattaaag aacttctgat
     7861 atgcttttat cctgatttgt acgtccaagg aaaactaaac catttagatg aaaaaattga
     7921 taatatcaat ctacaaatat tctgttgtct tctgtaagta taatttgctc tcatcgttct
     7981 ttcctataat acttaaataa agattctact atatctttta aagaataacg cggcttccat
     8041 tttgttagtg agtaaaatct gtttgaatcc attcccaatt ctaaatcaat cttttctgct
     8101 tttacattga ctataacatt acttccaagc atttcttttc caatcttagt agccatatca
     8161 gccatttcaa gaactgtata gctgccctcc atacctaaat tatatacagt ttcccactca
     8221 tcagaattag tcttcaataa tgcaataatt ccatcagcag catccctgat atcaagtctt
     8281 tgtagcttct gttttccatc aattacttca attgtttcat tatttataac attgataaca
     8341 aattttgaaa ttatatcaac tggtaccaag ccttcctgcc cgcctgctaa ggatgataat
     8401 cttaaagatg ttacattaga atgtttgctt ataagcttta tgttttctgc catcaactct
     8461 gtgcaatatt ttgcttgagc ataaatagtt tcaggcatta ccttagtttt ttctgtccag
     8521 taaatcgcct gtttagttcc ataaacactt tgtgatgaaa tattaataat ttccgggatt
     8581 tgatgaattg ccgcaagatt aaaaagctga ttagtaaaat ttaagctatc agtaatttct
     8641 tcgttgcctt tatgtggtcg tgcaaaacca caatgaatta ttgtatctac caatccgatg
     8701 cttaagctcc catttttaat atcatcatat tcgaaacatt taacaccttc aaaatgatta
     8761 tacaacttct caatcttgaa tttatttcta ctcaatgccc aaacttcata ctttgatttc
     8821 aataacttct taatgagagt ttgaccaagg taaccgctag cgcctgttac taaaattatt
     8881 ttacctgttt ttaactcttt tttattaata gataatgaaa gttcttttat tctcttgttt
     8941 ttattcaagg atattatttt atcagtagtt tgcctaattc caaatctacc ataatttagt
     9001 tttaaagttt cattcttatt agataaattt aaccaatcct taatacaaca ttctacctca
     9061 ttaaatccca taatagctct taatccggta atgccggtaa acctggcgtt catttcaaaa
     9121 agttttaatc ccttatctgt tagtctgccc tgtatattta aaggtcctct gtgtccaagt
     9181 tgtttcaaca taggtatcag cttatcaatt tcagaccata tgtattcatt atcataagga
     9241 ataatttcaa taggaacacc gttatttaat ttattatagg aagccattct ccccatcaaa
     9301 tcaccatttt tatttgtaac aatttgtatc gatatttcag ataattgtga attaactttc
     9361 ttagatattt gttgtaaata tgcttgccta aaagggtctc ctttgtgcgg tattgctaat
     9421 tcttgtatta tatgttgatt cgtgacgcga actaaatcct tttcttctaa tagtatttct
     9481 attcctttcg aagcatatcc atctctaggt ttagctatca aaggaaaact aatttcatta
     9541 ttttttatta ataatttaat ttcttcaaaa tcatagttct taacaaatac atcggaaatt
     9601 gaagatagtt catcacacat tttagcttta tctcttatta aagaaataag tttttcatct
     9661 gaaaccaaaa ccttaaaccc attttcttct aacatggatt tatgttcagc taaaatcaaa
     9721 gcctcatcat ctgagccggg aataattagg tctattttat gcttataaca cttatctatt
     9781 aattccggaa tataatcttt tgaataaata ataggcacat aatccattac atcacattca
//
LOCUS       DAOPUE010000068         9649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_7108_length_9649_cov_9.481968, whole genome shotgun sequence.
ACCESSION   DAOPUE010000068 DAOPUE010000000
VERSION     DAOPUE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7108_length_9649_cov_9.481968"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKH42_06875"
     CDS             complement(<1..419)
                     /locus_tag="PKH42_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA20192.1"
                     /translation="MEFDMLNLIMKRRSIRKFKDEKLSVKQIQELVNAGLLAPTSRNN
                     KAVELIVVDDTDTILKLKDCRNMGTTGLQTAPCAIVVIGDSEKSDVWVEDASISASYM
                     QLRAEDLGLGSVWIQMRKRVSDKDSSENEVKKVLNIP"
     gene            complement(401..532)
                     /locus_tag="PKH42_06880"
     CDS             complement(401..532)
                     /locus_tag="PKH42_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20193.1"
                     /translation="MSKVIIQECKNYSVDDIMGKINSGIEKLGGWKRKRRIQWNLIC"
     gene            complement(538..>1470)
                     /locus_tag="PKH42_06885"
     CDS             complement(538..>1470)
                     /locus_tag="PKH42_06885"
                     /inference="COORDINATES: protein motif:HMM:NF025608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009976 - tocopherol cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tocopherol cyclase family protein"
                     /protein_id="HOA20194.1"
                     /translation="KKKSKKYFEGWYFKQVSFDLKDIVSIIPGISINSADSHSFIQII
                     YLYDVDGYKNLKTQYYRFSVEDFKSTDRPFSLKIGNNTFRNEGIELNLNNGEFLIKGN
                     ICFSDFTKINRNILLPDAMGYFSYFPFMECYHDIISMSHKLKGFISVNNKVIDFNEGK
                     GYIEKDWGRSFPNEYIWLQSNHFEGNEASIMFSLAHIPFIGASFQGFICNLIINNHEY
                     RFATYNKSKIKNVSYSDNFLEIYIDKDEFELSIKAVVCRDSGMLRAPRKGAMDILIKE
                     GLSGAAEIRLLKNSKVVFEGKANPCAIEMMYRKE"
     assembly_gap    1471..1570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1581..2036)
                     /locus_tag="PKH42_06890"
     CDS             complement(1581..2036)
                     /locus_tag="PKH42_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007841798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOA20195.1"
                     /translation="MKYDAKTFEEYLAILPEDRKYPFKKLRDIVAENIPKGFKERYSD
                     SFLQFVVPHEIYPNGYHVNPKEPLPFIGIASQKHFIGFYHLGIYAFDDILKWFVEEWP
                     KHVTTKLDMGKSCIRLKKMDQIPYELIAELCQKITVDMWVEKYESSRKK"
     gene            complement(2226..3149)
                     /locus_tag="PKH42_06895"
     CDS             complement(2226..3149)
                     /locus_tag="PKH42_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOA20196.1"
                     /translation="MSTRMDFVRHSLELHLFFARIMKEHSFFLEVAFTPRDSNYTQKA
                     DEFRREFDRLLGEVVSLSNGVVSNSVLQSGEVITPYTQNAEAATSYFTGIRIPTELTQ
                     MEANLMGGGLTGFNPMMEERVYLLNQKAMALISDLIQFKSNILSNVLSCKMFTLNYPL
                     LIDHIMREAKLYLTMIQRLQNREVVNLQQEAAEQEIFWNRIMAEHAKFIRGLLDPTEE
                     QLFEAANNFGNEFDQLTREAVMALNSTVPLQQVTEDSLAATQAIRDFKVQGTQGILDC
                     KIKSIIIPLLGDHTIREANHFIRLLRMYRNI"
     regulatory      3281..3378
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3438..4334
                     /gene="metF"
                     /locus_tag="PKH42_06900"
     CDS             3438..4334
                     /gene="metF"
                     /locus_tag="PKH42_06900"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOA20197.1"
                     /translation="MKIIDKFTEKKPLISFEIFPPKADYPIDTIFNAIDELYNLDPDF
                     ISVTYGAGGSTKDKTVEISSYIQNRYNLTSIAHVTCITSARKEINKILISLIENNVHN
                     VLALRGDYPKDCKISPPIDGFKNSMELIEFIKSNSNLCVGAACYPEKHPAARSLNDDI
                     DFLKRKMEAGADFLVTQMFFDNDLFYDFYDKIKKKNIDLPIFTGIMPILNKNQINTIL
                     NLTGNSIPNKFKMLLEKYENNPEALKEAGMSYAVSQIIDLLSWGVDGIHIYTMNKPST
                     ARKILENISAIRSVLTHESKFY"
     gene            4315..5004
                     /locus_tag="PKH42_06905"
     CDS             4315..5004
                     /locus_tag="PKH42_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOA20198.1"
                     /translation="MKVNFIKKDLVVINKDEALRYMGYNSKTVDIETQKLLDESIAEV
                     KDLAELKYVYKIFDIEKENNNISFENVINIKSNDLYTLFKNCEKSAVIAATLGFEVEK
                     RIRYYSLTNLSKALVFDACATSCIEALCDVAQAEIKEIALKEGFNTTHRYSPGYGDVP
                     ISHQSEILSALNAQKLIGLSVLDSYILIPRKSVTAFIGFIKDSKIYKKSCIDCNLFGN
                     CNFSKEGDRKC"
     gene            4998..>7134
                     /locus_tag="PKH42_06910"
     CDS             4998..>7134
                     /locus_tag="PKH42_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOA20199.1"
                     /translation="MLNKLKENFLFFDGAMGTMLQNSGLKTGELPEELNITNPEIIYN
                     IHKEYLNAGANIITTNTFGANKLKLINSSYTVDELITAGVKTARKAIEGFNEKHVALD
                     IGPIGTLLSPLGSLSFEEAYDLFKEQVTIGVNAGIDLILIETMTDIYELKAAILAAKE
                     NSILPVFATMSFQDKGRTFTGNDVKSFVFTAEGLGVDVLGVNCSLGPKQLQPIVDELL
                     KYSSIPIMVQANAGLPKYRNNTAYYDIDPSKYSHEINIMAKKGVAIFGGCCGTTPEYI
                     KSIVNALDGLKPIKIVKKNFTTAASSTKTVFLGDEVKIIGERINPTGKKRLKEALREN
                     NLNYIVREAIEQEDSGSDILDINVGLPGIDEKEMMKKVIKEVQSVVSLPLQIDSADYE
                     VIEAAARIYNGKPIINSVNGKKESMDKIFPIAKKYGALVVALTLDEEGIPATSEKRLE
                     IATNIIETAKVYGIPEENILIDPLVLTASAEQKAVMETLKAIPLIKNKYNVKIVLGTS
                     NVSFGLPKRNLLNTTFLSMALGFGLDAPITDSTNKSLMDVVRSFKVLANQDKDSKDYI
                     KTYTNISYENNIPSSETDLKEIIIKGLKDEAKKVSIKMLEQYNSLDIVSEFMIPALDI
                     VGEKYEKEEIFLPQLIRSAETSKIVFEVIKEKSIKEEITDEKKILLATVKGDIHDIGK
                     NIVKLILENYGYKVIDLGKDVDIENIVETV"
     assembly_gap    7135..7234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7235..7443)
                     /locus_tag="PKH42_06915"
     CDS             complement(<7235..7443)
                     /locus_tag="PKH42_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOA20200.1"
                     /translation="MTYTVLFFTHSGAIKFNRKCKKNNINCELMPVPRALSSNCSISA
                     KIEYYDNIDNLIDDEIEKIFLTQNN"
     gene            complement(7443..7649)
                     /locus_tag="PKH42_06920"
     CDS             complement(7443..7649)
                     /locus_tag="PKH42_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOA20201.1"
                     /translation="MKKIDTCGMSCPQPVLMTKKALEESPEGLDIIVDNNTAKGNVER
                     FLKNSGYKVIITENEDNYILEARK"
     gene            complement(7662..8741)
                     /gene="yedE"
                     /locus_tag="PKH42_06925"
     CDS             complement(7662..8741)
                     /gene="yedE"
                     /locus_tag="PKH42_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOA20202.1"
                     /translation="MSDKKGIIIAGGAVGIISVLLVYFGNPANMGFCIACFLRDIAGG
                     LGLHRAEVVQYIRPEIIGLVLGAFLISVKNKEFQTKGGSSPFTRFMIGIAVMIGALIF
                     LGCPLRMVLRLAGGDLNALFGIAGFVAGIFVGVIFLNKGFNLKRSYKISKTEGYIFPV
                     LVLGLLILLIASPSFLFFSEKGPGSMAAPIIMSLTAGLIVGGLAQKTRLCMVGGTRDM
                     ILFKDMYLLSGFIAILVFSFIGNLAFGFFKLGFAEQPIAHTDGLWNFLGMAVVGWGSV
                     LLGGCPLRQLILSGEGNTDSVITVMGMLVGAAIAHNFGLASSAAGVTANGKVAVFICF
                     ALLGIVSFLNSESLVKAGNLSKASN"
     gene            9021..>9649
                     /locus_tag="PKH42_06930"
     CDS             9021..>9649
                     /locus_tag="PKH42_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA20203.1"
                     /translation="MRYVYMDNGATSFPKAPGVAESMADYILNVGTNVNRGAYSSSFK
                     AENIVYETRELLCELFRFNKPENVIFTKNITESMNVLIKGLLKEKDHVIVSSMEHNAV
                     MRPLNSLNKVEYTKVLCSKTGVLSIEDVENSIKPNTKAIIMTHASNVCGTILDLEKVG
                     QLCKKHNLFFIIDSAQTAGSIDIDINKLNADAIGFTGHKSLLGPQGIGG"
BASE COUNT         3243 a         1633 c         1480 g         3093 t
ORIGIN      
        1 tcaggtatat ttaatacctt tttgacttca ttttctgagc tgtctttgtc acttaccctc
       61 tttctcattt gtatccaaac cgagcctaat ccgaggtctt cagctcttaa ttgcatgtag
      121 gaagctgaaa ttgatgcatc ttctacccat acatcacttt tttcactgtc gccgataact
      181 acaattgcac aaggcgcggt ctgcagtcct gtggttccca tattcctaca atcctttaat
      241 ttaagaattg tatcagtatc atcgactaca ataagttcaa ccgctttatt gttccttgat
      301 gttggagcta acaatccggc attcaccaat tcttggattt gttttactga tagtttttca
      361 tctttaaact ttcttataga ccttcttttc attattaaat tcaacatatc aaattccatt
      421 gtatcctcct tttccttttc catccgccta atttttcaat accactgttt attttaccca
      481 ttatatcatc tacagaatag tttttacatt cttgaattat aactttgctc atttaaatca
      541 ttcctttctg tacatcattt ctatagcaca tggatttgct tttccttcaa aaacaacctt
      601 cgaattcttt aataatctta tttctgctgc accggataat ccttctttga ttaaaatatc
      661 catagcaccc tttctcggtg cccttagcat ccctgaatcc cggcaaacaa ctgctttgat
      721 actaagttca aattcatcct tatctatata aatctccaaa aaattatccg aatagctgac
      781 gttttttatc ttgcttttat tgtaggtagc aaatctgtac tcatgattat taataattaa
      841 attgcaaata aacccttgaa aagatgctcc gataaacggt atgtgggcaa gcgagaacat
      901 aatacttgct tcatttcctt caaaatgatt cgattgaagc catatatatt cattagggaa
      961 tgatctaccc cagtcttttt ctatataacc tttaccttca ttaaaatcaa taaccttgtt
     1021 attaaccgaa ataaaacctt tcagcttatg gctcatgcta ataatatcat gatagcattc
     1081 cataaaagga aagtatgaaa aataccccat tgcatccggt aataatatat ttctgttaat
     1141 cttagtaaaa tctgaaaaac aaatatttcc ttttattaaa aattcaccgt tatttaaatt
     1201 aagctctata ccttcattcc taaatgtatt atttcctatt tttaaactaa aaggtctatc
     1261 cgtgctttta aaatcttcga cagaaaacct ataatattga gttttaagat ttttataacc
     1321 atcgacatca tataaatata taatttgaat aaaagaatga gaatctgcgc tgtttattga
     1381 aataccgggt ataatactga ctatatcctt taaatcaaat gatacttgtt taaaatacca
     1441 tccttcaaaa tattttttgc ttttcttctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn caattgccat ttatttcttc ctggaacttt catacttttc cacccacata
     1621 tcaacggtta ttttttgaca aagctctgca atcagctcat atggaatttg gtccattttt
     1681 tttaacctga tgcagctttt tcccatatca agcttcgtgg taacatgttt tggccattct
     1741 tcaacaaacc attttaatat atcatcaaaa gcatatattc ctaaatggta aaacccgata
     1801 aaatgcttct gagacgctat gcctataaaa ggaagaggtt cttttggatt tacatgatac
     1861 ccattaggat atatttcatg aggaacaaca aattgtaaaa agctgtctga atagcgctct
     1921 ttaaaaccct tagggatatt ttccgctaca atatctctta gtttcttaaa cggatatttc
     1981 ctgtcttcgg gcaagattgc cagatattct tcaaatgttt ttgcatcata tttcatgatt
     2041 tttctcctct tttataatat aacataaatt atacccgcta tgatatttta gaaacataag
     2101 tatgcagccc tttctacata tattatagcc gaatatcatt ttattacaat cattaacttg
     2161 tttgggtaaa caaaaaatag tgcagtatca ttacagatac tacactgtgc tctttccatt
     2221 tcatactaaa tgtttctata cattcttaaa agtcttataa agtgatttgc ttcacgaatt
     2281 gtatggtcgc cgagaagcgg aattatgatt gatttaatct tgcagtccag tatcccttgt
     2341 gtaccttgga ccttgaaatc ccttattgcc tgagttgccg ccagactgtc ttctgtaact
     2401 tgttgcaagg gcacagttga attcaaagcc attacagcct ctctcgtcag ttggtcaaac
     2461 tcatttccaa aattattggc tgcttcaaac agttgttctt cagttgggtc aagcaaccct
     2521 cttataaact tggcatgctc tgccattatc ctgttccaga atatttcttg ctccgcagct
     2581 tcttgctgca aattaacaac ctctctgttt tgaagccttt gtatcatagt taaatacagc
     2641 ttagcttcac gcataatatg gtcaattaat aagggataat ttagtgtaaa catcttgcat
     2701 gataaaacat tgctcaatat attgctttta aactgtatca aatctgatat taatgccatt
     2761 gctttctggt ttaataaata aaccctttct tccatcattg ggttgaaacc ggttaagccg
     2821 cctcccatca aattagcttc catttgcgta agctctgtgg gaatccttat tcctgtaaaa
     2881 tacgaggttg ctgcttcagc attttgtgta taaggggtta taacttctcc tgattgaaga
     2941 acgctgttgc ttacaactcc atttgaaagt gatacaacct ctcctaagag cctgtcaaat
     3001 tctcttctga attcatctgc tttttgtgta taattactat cccttggggt aaatgctact
     3061 tcaaggaaaa aagagtgttc cttcattatt cttgcaaaaa aaaggtgcaa ttctaatgaa
     3121 tggcgaacaa aatccattct tgtagacatc ataatcctcc ttaaaaattt aatgtttaca
     3181 atatattatg aatcagaaga caaattgctt acaaaatatt taatttatct attgacaatt
     3241 aggtgataat aattatatac tttgatttaa taatttaata aattcatcaa gagtgacgga
     3301 gggactggcc cgatgaagtc cggcaaccaa tctatgtata tggtgctaat tccagcggtt
     3361 tacaccggca gatgaggatt tttaagacaa tttctttccc aatgtcggga gagatttttt
     3421 atttattggg aggatttatg aaaataattg ataagtttac agaaaaaaaa cccttaatat
     3481 catttgagat attcccgcca aaggcagatt atccgataga tacaattttt aatgctatcg
     3541 atgagcttta taatttagac ccggatttta tcagtgttac ttatggtgca ggcggaagca
     3601 ctaaagataa aactgttgaa atatcatcgt atatacaaaa ccgttacaat ttaacttcaa
     3661 tagctcatgt tacttgtatt acttctgcaa gaaaggaaat taataagata ttaatatcat
     3721 taatagaaaa taatgttcat aacgtcttgg ctttaagagg agattaccca aaagactgta
     3781 agatatcccc gccaattgac ggctttaaaa attcgatgga actgatagaa tttataaaaa
     3841 gtaattcaaa tttatgtgtt ggagccgcat gctatccgga aaaacatcct gctgcaagaa
     3901 gtttaaatga tgatattgat tttcttaaaa gaaaaatgga agcaggtgca gattttttgg
     3961 taacacaaat gttttttgac aatgacttgt tttatgattt ttatgataaa ataaaaaaga
     4021 aaaatattga tttgcctata tttaccggta ttatgccaat acttaataaa aaccaaataa
     4081 atacgatatt aaatcttaca ggtaattcaa tacctaataa atttaaaatg ctgctagaga
     4141 aatatgaaaa taaccctgaa gctttaaaag aagcaggaat gtcatatgct gtcagtcaaa
     4201 taatagacct tttgtcttgg ggagttgatg gcattcatat ttatactatg aataaacctt
     4261 ccaccgcaag aaaaatatta gaaaatattt ctgcaatcag gagtgtactg acacatgaaa
     4321 gtaaatttta ttaaaaaaga tttagttgta attaataaag atgaagcttt gagatatatg
     4381 ggatacaaca gcaaaactgt ggatatagaa acccaaaaac ttcttgatga atcaatagct
     4441 gaagtaaaag atttggcaga acttaaatat gtatacaaaa tatttgatat cgagaaggaa
     4501 aataataata tatcgtttga aaatgtgata aatatcaaat caaatgactt atacaccctt
     4561 tttaaaaact gtgaaaaatc ggctgtaata gctgctaccc ttggttttga agttgaaaaa
     4621 aggattaggt attacagtct gactaattta tcaaaagctc ttgtatttga tgcatgtgcc
     4681 acttcctgta ttgaagcatt gtgtgacgtg gctcaagctg aaataaaaga aattgcttta
     4741 aaggaaggat ttaatactac acatagatac agtcccggct atggagatgt gcctatttcc
     4801 catcaatctg aaatactgtc tgccttaaat gcccaaaagc ttatcggttt gtctgtttta
     4861 gattcttaca tattgattcc gagaaaatcg gtgacagctt ttataggttt tattaaagac
     4921 agtaaaatat ataaaaaatc ctgcatagac tgcaatttat ttggaaactg caatttttca
     4981 aaggaaggag atagaaagtg ttaaataaat taaaagagaa tttcttgttt tttgacggtg
     5041 caatgggtac catgcttcaa aattccggat taaaaacagg tgaactgccg gaagaattaa
     5101 atattacaaa tcccgaaatt atttataata ttcataaaga atatttaaat gcaggagcta
     5161 atattataac tacaaataca ttcggtgcaa ataagcttaa attaataaat tcttcataca
     5221 cggttgatga attaataact gcaggtgtaa aaacagctag gaaagctata gaaggtttta
     5281 atgaaaaaca tgttgcctta gatatcggtc ccataggtac cctcctgtcc ccattaggaa
     5341 gtctaagttt tgaagaagct tatgatttat ttaaagagca agtaacaata ggagtcaatg
     5401 caggtataga tttaatactt attgaaacga tgaccgatat atatgagtta aaagccgcaa
     5461 ttcttgcagc taaggaaaat tctatactcc ctgtttttgc aacaatgtct tttcaagata
     5521 agggtcgaac atttacgggt aatgatgtaa aatcatttgt ttttacggct gaaggtttgg
     5581 gcgtcgatgt tttgggcgtt aactgctcct tagggcccaa acagcttcag ccgattgttg
     5641 atgagttgtt aaaatacagc tcaataccaa taatggtaca ggcaaatgcg gggctgccta
     5701 aatatcgcaa caatactgct tattatgata tcgacccttc aaaatacagt catgaaataa
     5761 atataatggc taaaaaaggt gttgcaatat ttggaggatg ttgcggcact actcctgaat
     5821 atattaaaag tattgttaat gcattggatg gattaaaacc gataaaaatc gtaaaaaaga
     5881 actttacgac tgctgcttca tcgaccaaga cagtattttt aggggatgaa gttaaaatta
     5941 tcggcgaaag aattaacccg acaggtaaaa agagattaaa agaagccctt agagaaaata
     6001 acctgaacta tattgtaaga gaagctatag aacaagagga ctcaggctct gatatattag
     6061 atatcaatgt gggacttccc ggtattgacg aaaaagagat gatgaaaaag gtaatcaaag
     6121 aagtgcaaag tgttgtttca ttgcctcttc aaattgatag tgctgattat gaagttattg
     6181 aagctgccgc aagaatatat aacggaaagc ccataattaa ttctgtaaac ggcaagaaag
     6241 aaagtatgga taaaatattt ccgattgcaa aaaaatatgg ggccttggta gtggctttaa
     6301 cactagatga ggaaggtatt cctgcaactt ctgaaaaaag attggaaata gcaacaaata
     6361 ttattgaaac agcaaaagta tacggtattc ctgaagaaaa tatattaata gacccgctcg
     6421 ttcttacagc ttctgccgag cagaaagccg taatggaaac acttaaagca atccctttaa
     6481 ttaaaaataa atataatgta aaaatagtat tgggaacaag caatgtttct tttgggctgc
     6541 ctaagagaaa tttattaaat acaacttttt tatctatggc tttaggtttt ggtttggatg
     6601 cacctattac ggattcaaca aataagtctt tgatggatgt tgtaaggtcc tttaaggtac
     6661 tggccaatca agataaggac agcaaggatt atattaagac atacacaaat atatcctatg
     6721 aaaacaatat tccttcatct gaaaccgact taaaagaaat tattataaaa ggtctaaaag
     6781 atgaagctaa aaaagtttct ataaaaatgt tggagcaata taactcgctt gatatagtaa
     6841 gtgaattcat gatacctgcc ttggatattg taggtgaaaa atatgaaaaa gaggaaatat
     6901 ttctgcctca gttaataaga agcgccgaaa catccaaaat agtatttgaa gtaataaagg
     6961 aaaaatcaat aaaagaagaa ataaccgatg agaaaaaaat actattagca acggtcaagg
     7021 gagatattca cgatataggg aaaaatatag ttaagcttat acttgaaaat tacggttaca
     7081 aagttattga cttaggcaag gatgtggata tagaaaacat tgttgaaacc gttannnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatatta ttttgagtta aaaatatttt
     7261 ttctatttca tcgtcaatta aattatcaat attgtcataa tactctattt ttgcacttat
     7321 actgcaatta gaactcaaag ctctaggcac gggcataagc tcgcaattaa tattgttttt
     7381 cttacatttt ctattaaatt taatggctcc tgaatgcgta aaaaacaaaa cagtatatgt
     7441 cattatttcc ttgcctctaa tatgtaatta tcttcatttt ctgttatgat taccttatag
     7501 cctgaatttt ttaagaatct ttccacattc cctttagccg tattgttatc tactattata
     7561 tccaatcctt caggactttc ttccaatgct ttttttgtca ttaatacagg ctgaggacag
     7621 ctcatgccgc atgtatctat ttttttcata taatcctccc cttaattact tgcttttgat
     7681 aaattacctg cttttactaa gctttctgaa tttaaaaatg aaacaatacc taataatgca
     7741 aaacatataa atacggcaac ctttccgtta gcagttactc ctgctgcact tgaagctaat
     7801 ccgaaattgt gtgctatagc cgctccaacc agcattccca ttacagtgat aacagagtct
     7861 gtattgcctt cgcctgatag tattaattgc ctaagcggac atcctcctaa taataccgag
     7921 ccccaaccta ctactgccat acctaaaaag ttccataggc cgtcagtatg tgcaataggt
     7981 tgctcagcaa atcccaattt gaaaaatccg aaagctaaat ttccaataaa actaaatacc
     8041 agtatagcaa taaatcccga taaaagatac atatctttaa aaagtatcat atcacgagtt
     8101 ccgccgacca tgcacaatct tgttttttga gctaatccac caactatcaa gcctgccgtc
     8161 agcgacatta ttataggtgc agccattgag ccgggacctt tttcactaaa gaacaaaaag
     8221 ctgggtgaag caataagaag tattaaaagt cctaatacta gaaccgggaa aatatagcct
     8281 tcggtttttg atattttata gcttctctta agattaaacc ctttgtttaa gaaaataact
     8341 ccgacaaata ttcctgcaac aaagccggca attccgaata aagcgtttaa atctcctccc
     8401 gcaagcctta aaaccattct tagcgggcat cctaaaaata ttagtgcgcc aatcataact
     8461 gctatgccta tcataaatct tgtaaacggt gatgaaccgc ctttagtttg gaattcttta
     8521 tttttaacgg atattaaaaa tgctcccagt acaagaccta taatttcagg ccttatgtat
     8581 tgaacgactt ctgccctatg taatcctaat ccccctgcta tatctcttaa aaaacaagct
     8641 atacaaaagc ccatatttgc aggattccca aaatatacaa gtagtactga tattattccg
     8701 actgctccgc ctgctataat gattcctttt ttatcactca ttagctctcc tcctattatt
     8761 aataattaat aaattaacaa taagattata tgatggaaaa ttgcttaaaa caatatgtat
     8821 agaattttac aatgcttcga gaagcaacta ttaatattgc tgatattaaa atagaaaacc
     8881 ttgaattata aacattttca aaatttatat aataaagcat taatgcaacc tattaaaatc
     8941 tttaattgaa caaatattta ccatatgtta aaataatatt gtttagaata aaattagaaa
     9001 atatattaga ggactatact atgagatatg tctatatgga caatggagcc acctcttttc
     9061 ctaaagcacc cggtgttgcg gaaagtatgg cagactatat attaaatgtg ggaactaacg
     9121 ttaatcgagg agcttacagt tcatctttta aagctgaaaa cattgtatac gaaaccaggg
     9181 agctcctatg tgaattgttc cgttttaata agcctgaaaa tgtaattttc actaaaaaca
     9241 taaccgaaag catgaatgta cttatcaaag gattattgaa agaaaaggac catgtaatag
     9301 tttcttctat ggagcacaat gctgttatga gacccttaaa ttcacttaat aaggttgaat
     9361 atacaaaagt tttgtgcagt aaaacaggag tattaagtat agaagatgtg gaaaactcta
     9421 ttaaaccaaa tactaaagcc attataatga cccatgcttc aaatgtatgc ggaactattt
     9481 tagatttaga aaaagttgga caactttgca aaaaacataa tttattcttc ataatagaca
     9541 gcgctcaaac agcagggtct attgatatag atataaataa actaaatgcg gatgcaattg
     9601 gatttacagg ccacaaaagt ttactgggtc ctcagggaat aggcggatt
//
LOCUS       DAOPUE010000069         9568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_7183_length_9568_cov_10.367602, whole genome shotgun sequence.
ACCESSION   DAOPUE010000069 DAOPUE010000000
VERSION     DAOPUE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7183_length_9568_cov_10.367602"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKH42_06935"
     CDS             <1..1020
                     /locus_tag="PKH42_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOA20204.1"
                     /translation="KMQEEGFKFNNFYTPTWGVSTSDGEYVACTGLLPKEGIWSFYKS
                     SKNYMPFAMGNQLKKEGYETKAYHNHYFDYYYRHKSHPNLGYTYKGLGNGLNVTETWP
                     ESDLEMIELTVPEYIDSQPFHVYYMTVSGHLQYTFTGNYIAAKNRSLVENLPYSKNVK
                     AYLACNIELDKAMEKLIQELEKKGIAEDTLIAISPDHYPYGLTLNEISELAGKEIKSD
                     VDLDRGIFILWSKGMEPVKIDKVCSSLDIIPTLSNLMGLEYDSRLLMGRDIFSDSEPL
                     VMFSDRSFITDKAFYNARTNEVTSFTDENIESSYIKETAETVINKFKYSSMILDTDYY
                     KIILE"
     gene            complement(1069..1923)
                     /locus_tag="PKH42_06940"
     CDS             complement(1069..1923)
                     /locus_tag="PKH42_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20205.1"
                     /translation="MKECTIYIDVPEKFLKGAGIPNSRFIPSYIYQIKIILKNNSSQK
                     KQCSFSATMGNGIRYLENIKHEGPDINLLDTVSAVEPCKELGNDNVIIFGNNLTLSPN
                     STNTIIFDGALCDKYTINSIENSGDKIPHRSKINFYAHLSDEDKIDDSYFISSTAMDS
                     DMIVKCDDEKLNAGDITKYYVDICTGQYDLARKVYLRSILDAGLEYIGDSCNLEPRNI
                     YEFNGKTIIKWDIGSLQPSEIKRIGYKVKVKNDAKGKLTNKLNSNCINNSTYTQCPVS
                     CKYTIEVC"
     gene            2077..2301
                     /locus_tag="PKH42_06945"
     CDS             2077..2301
                     /locus_tag="PKH42_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOA20206.1"
                     /translation="MKKIKIYTGSSCSNCTAAKEYIKEKGYEYEEKNISADSEAKKEL
                     IALGYMGVPVIMIDDEVVVGFNKSKLDEIL"
     gene            complement(2513..2695)
                     /locus_tag="PKH42_06950"
     CDS             complement(2513..2695)
                     /locus_tag="PKH42_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOA20207.1"
                     /translation="MITKDMTIREVIMKNQEAAEILMTFGMGCVGCPSAQMETLEEAA
                     AVHGMNLDDLLAALNK"
     gene            2933..3838
                     /locus_tag="PKH42_06955"
     CDS             2933..3838
                     /locus_tag="PKH42_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20208.1"
                     /translation="MHNKYNEKISDMKKCIQKKNKLNLLLKQTEQDIIKEKLLLNKLS
                     GELEKEYQDALKLKSDNIISLFYEILGTEEDKHNKEKQKLLKARLKYEQCKNNMNYLV
                     NETKKIVDCIEKLNGCDAEYEELINKKIETINIEDNETGQALKKLIKRKENMNANIIE
                     ICEAICYGEKALEAIEMTIKELEAAENFENSDGSNNIENARGYAEQTHRMLGRFKKEL
                     SDITIVTGTEIAVGTFENFADCFFDNLIFDWVVQSGIRKSLDTVKNTKNQLDKAMSKL
                     YEEKVTEEFMIKQIEEQINQIIEKV"
     gene            4095..5078
                     /locus_tag="PKH42_06960"
     CDS             4095..5078
                     /locus_tag="PKH42_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20209.1"
                     /translation="MSEYVLEIRDLSICFYSGRKEIPAVENLSFNLSRGETLGIVGES
                     GSGKTVTSLSIMKLIPNPPGKITGGEMFFGSLDLLKVDEDTIRQIRGNNISMIFQEPM
                     TSLNPVYTIGEQLSEVFCTHQKLSKKEAMKKSLDMLKQVKIPLAEKRMKQYPHELSGG
                     MRQRVMIAMALACMPEILIADEPTTALDVTIQSQILHLIREMKEKMNTAVILITHNMG
                     VVAEMADNVMVMYCGRALEYADVRTLFKEPKHPYTIGLLNSIPRIKGGRKKLYAIKGS
                     VPVPGEITAGCRFNSRCEYVFEKCLVEEPPIFEVGKSKVRCWKYEGKGVTV"
     gene            5075..6058
                     /locus_tag="PKH42_06965"
     CDS             5075..6058
                     /locus_tag="PKH42_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA20210.1"
                     /translation="MSGNILEVKNLKVHFPVKGELIPKNVGYVKAVDGISFNIGYAET
                     LGLVGESGCGKSTTGRAVLNMLKVDSGQVFFEGKDILKSSRKELKKIRRDMQLIFQDP
                     FGSLNPRMTIGSALEEVLYIHHIRDKNERQKRVIELIEMVGLNKNYLSRYPHEFSGGQ
                     RQRAVIARALAVKPKFIVCDEPVSALDVSIQSQIINLLEELQKNFKLTYLFIAHDLSV
                     VKHISNRIAVMYLGRIVEIAESDELFKRPAHPYTKALLSSIPVCDPDFRKNIILLEGD
                     LPSPANPPKGCRFHTRCIYAREDCSRKEPVSVNLGNGHMVECHLYSNEEED"
     gene            6063..7664
                     /locus_tag="PKH42_06970"
     CDS             6063..7664
                     /locus_tag="PKH42_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20211.1"
                     /translation="MKKNISKMFALVLILSCTALVFSGCSKNDVPVGETPEGNNGEQP
                     AKETTLVIAYERDAETLNHIKTGWYSDSLIYMYDRLVSRDYDFNYRPSLAISWETSED
                     GMVWTFHLRDDVKFHNGKPLTAHDVKFTFDTILDPETASPSQSDFAAIKGLEVKDEHT
                     VDIMLHQPFPNLLFVLSNTAAGIVNKEAYEEYGDEYGINYVVGTGPYMFKEWKQGDKI
                     IIVKNPDYNWGPEWMTNRGPALIDRIEFRTIPEENTRLMELEAGNVHILRDVPASFLQ
                     RVEEIDDVEVMRRDATKLGYLAYACDKEPFTDIRVRRAINHAINKEDIIKYVFRGYGE
                     PAYGYLPPALKSEYYADSKKDGYEYDVEKAKQLLKEAGYGDGLKLTLSAENSTEYSRL
                     AEVLQDQLKQVGIETEIQLYDSSSYTSMLREGQQELFIRLYSWPNADILDWFLLSSQF
                     PYPNHSRWQDEKTDELINKAATSPTWDERSKGYFEVQKHLIEQAVWAPIYIPENTIAV
                     RKEVKDFKFHPWIPQYNDGTYLEVE"
     gene            7725..8642
                     /locus_tag="PKH42_06975"
     CDS             7725..8642
                     /locus_tag="PKH42_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20212.1"
                     /translation="MLKYIFKKLLMMIPILLGLTIILFLILHLAPGDPVHLVVGPNVT
                     PEVYENVRRSMGLDRPLIIQYFDYLSNLVKGDLGSSILQHRPVADIVLERFPITIGLG
                     VRALLLSFLIAVPVGIIAAVNRNKSADFIAMTFALVGISMPTFWFGIILLYFFAYKLG
                     LFPISGYGTWKHLVLPVITMGLTDAAVIARMMRSSMLEVIGQDYIRTARAKGLREKVV
                     TNKHALKNALIPVITLLGMRMGWIIGGSVTLEIIFSIPGLGRTMVDSIFSRDYPVVQG
                     SVLILATSVMISNLIADILYALVDPRIRY"
     gene            8684..9565
                     /locus_tag="PKH42_06980"
     CDS             8684..9565
                     /locus_tag="PKH42_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20213.1"
                     /translation="MDDIIIEDNLEDEVESWEPNFWERLFSNKMAVFGATVILILFIT
                     AIFAPLIAPYPYDAIDLPQMLKAPNSENILGTDEFGRDIFSRIVYGSRISLQVGFIAV
                     GISLVIGTILGALAGYYGGITDYIISGITDIAYSFPISLLAIAFVAALGRSLTNLIFA
                     VALVSWSGYARLVRGQFISLKNREFIEATKILGMSDARIIFKHMLPNSMAPVIVLTTL
                     EIPKAIIVESSLSFLGLGVPPPAPSWGSIMSSGRAYIMEAPWVSIFPGLVMALAVLGF
                     NLFGDALRDTLDPRLKD"
BASE COUNT         3185 a         1470 c         2012 g         2901 t
ORIGIN      
        1 aaaatgcaag aggaaggttt taagtttaat aacttttata ctcctacctg gggagtctct
       61 acctccgacg gtgaatatgt agcttgtacg ggacttttgc caaaggaagg catatggagc
      121 ttttataaat cttccaaaaa ctatatgccc tttgcaatgg gaaatcagct taaaaaagaa
      181 ggttatgaaa ccaaagccta tcacaaccat tattttgatt attattacag acataaatct
      241 catcctaact taggttacac atataaaggt ttgggaaacg gtcttaatgt aacagaaacc
      301 tggcccgagt cggatttgga aatgattgag ctaacggtac cagaatacat agattctcaa
      361 cctttccacg tgtactatat gacagttagc ggccatttgc agtatacctt tacaggcaac
      421 tatatagctg ctaaaaacag aagtctggtg gaaaatttgc catactccaa aaatgtaaag
      481 gcatacttgg catgtaatat cgagttggat aaagcaatgg agaaactgat acaggagctg
      541 gagaaaaaag ggattgccga agatactctc atagcaataa gtcctgacca ttatccttac
      601 ggtttgacat tgaatgaaat aagcgagctt gccggtaaag aaatcaaatc agatgttgat
      661 ttggatagag gaatttttat tctttggagc aagggaatgg agcctgttaa aatagacaag
      721 gtttgttcaa gtttggatat aattcctacc ttgtcgaatt taatggggtt ggaatatgat
      781 tcaaggcttc ttatgggaag ggatatcttt tcggattcag agcccttagt tatgttttcg
      841 gacaggagct tcattacgga caaagcattt tacaatgcca gaacaaacga ggtaacttca
      901 tttaccgatg aaaatattga aagctcctat attaaagaaa cagcggaaac agttataaat
      961 aaatttaaat attcatcaat gatattagat actgactact ataaaataat attagagtaa
     1021 aacaggagac gatatcttta ttttaattga acgtctcctg attttctttc aacatacctc
     1081 aatagtgtat ttacagctta ccggacattg agtataggta gagttgttta tgcagtttga
     1141 attaagttta tttgttagtt taccttttgc atcatttttt actttaactt tgtatccgat
     1201 tctttttatt tctgaaggtt gaaggctgcc tatatcccat tttataatgg ttttgccgtt
     1261 aaattcatat atatttctcg gttctaaatt acacgaatca cctatgtact caagtcctgc
     1321 atccagtata cttcttaaat aaacttttct tgctaagtca tattgtcctg tgcatatgtc
     1381 aacataatac ttagtaatat cgcctgcatt tagcttttcg tcatcgcatt ttacaatcat
     1441 atcggagtcc atagcagttg acgaaataaa atatgagtca tcgattttgt cttcatctga
     1501 taagtgagca taaaaattga ttttgcttct gtgaggtatt ttatcacctg aattttcaat
     1561 gctgttaatt gtatacttgt cgcatagtgc tccgtcaaat attattgtgt ttgttgaatt
     1621 gggggataga gtaagattgt ttccgaaaat aattacatta tcgttgccca attctttaca
     1681 tggttcgacg gcacttactg tatctaaaag gttaatgtca ggaccttcat gcttgatatt
     1741 ttccaaatac ctgattccat ttcccatagt agcgctaaag gagcattgtt tcttttggct
     1801 gctgttattt ttaagaataa tttttatttg ataaatataa gagggaataa accgggaatt
     1861 tggaatacct gcacccttca aaaacttctc gggcacatct atgtatatgg tacattcttt
     1921 catttatttc aatcctttct gctaatctaa ttcatattat tccgagccat ggtttttgtg
     1981 aaaaatttaa gttaaatatt tatggtatta aatgaagtaa tatggtaaaa taaattaaaa
     2041 attaggcgat atgcatacta acaacaggag gataaaatga aaaaaataaa aatttatacc
     2101 ggctcatcat gttcaaattg cactgctgca aaggaataca ttaaggaaaa aggatatgaa
     2161 tatgaggaaa aaaatatttc cgctgatagt gaagcaaaaa aagaattaat tgccttggga
     2221 tatatggggg ttccggtaat tatgattgat gatgaagtag ttgtaggatt caataaaagc
     2281 aagttggatg aaatattgta agggttgttt atgagtttta ttgaagagtt acagatagaa
     2341 catgatgcag tttacattat taactaaatg gctaagatat ttacatatta atatttttaa
     2401 taatgtaaat aatataaata agcaatataa acagctgttt tattgctttt aaatataaaa
     2461 accggcttta tgccggtttt tgctgggcag attgactgct tgttgtaaag tcttatttat
     2521 tcaaggcagc taataaatca tctaaattca ttccgtgtac tgctgcggct tcttccaatg
     2581 tttccatttg agcacttggg cagcctacac atcccattcc aaatgtcatt aatatttcgg
     2641 cagcttcctg gtttttcata ataacttcac gaattgtcat gtcttttgta atcatttatt
     2701 gcctcctttt aatttataat tcattataca gtaagataag atattattca atattttaat
     2761 taataaaaag ttacctgact ctatttgata attttgtatc aagaatagtt tttaataata
     2821 aataaaaacg tattcccgtt ttttcagact aaaaactgtt tgaatcgcca taagatgtat
     2881 gatataatca aaaaagttaa cagtaacaat agcaaagggc gggagcaaga atatgcataa
     2941 taaatacaat gaaaaaataa gcgacatgaa aaaatgcata caaaaaaaga ataaattaaa
     3001 tttgctttta aaacaaacgg agcaagatat tattaaagaa aaacttcttc taaataagct
     3061 ttcgggcgaa ttggagaagg aatatcagga tgctttaaag ctaaaatcag ataatattat
     3121 ttcgttgttt tatgaaatat tggggacgga agaagataaa cacaacaagg aaaagcagaa
     3181 gctattaaaa gcaaggttaa aatatgaaca atgtaaaaac aacatgaatt atctagttaa
     3241 cgaaactaaa aagattgtag attgtataga aaaattaaac ggatgcgatg ctgagtatga
     3301 agaattaata aacaaaaaga tagaaactat taatatagag gacaatgaaa ccggccaggc
     3361 tttaaaaaaa ctaataaaaa gaaaagaaaa tatgaatgcc aatataatcg aaatttgcga
     3421 agcaatttgt tatggtgaaa aagcactgga agctatagaa atgacaataa aagagttaga
     3481 agctgctgaa aactttgaaa attcagatgg aagcaacaat attgaaaatg caagggggta
     3541 tgcggagcaa acccatagaa tgttagggag attcaagaaa gaattatccg acataacaat
     3601 tgtgactgga actgaaatag cagtaggtac ctttgaaaat tttgcagact gtttttttga
     3661 caaccttatt tttgactggg ttgttcagtc cggaataaga aaatcattag atacagtaaa
     3721 aaatactaaa aatcaattag acaaggcaat gtcaaaacta tatgaagaaa aagtaacaga
     3781 agaattcatg ataaaacaaa tagaagaaca aataaatcaa ataatagaaa aagtataaag
     3841 gtattgacct attatattat ttagtgatac aattgcaata ctaaattaat aatagcgttg
     3901 tctaaattta catttctaat ctatcacttt ctaaattaat ttacattaat aattcttatt
     3961 aaaaatagtt gatttcacaa tacaggaaaa tgttctcata aagattgctg tttgatttga
     4021 tcttttattt atattcggag aagcatagtc tcctgcaggt tattattttt attttgaaga
     4081 ttaggtgatt gactatgagc gagtatgtgt tggaaataag ggatttgagc atttgttttt
     4141 acagcggcag aaaggagata cctgccgtag agaacctcag ctttaatctt tcaaggggag
     4201 aaacacttgg tatagtagga gagtccggct caggcaagac tgtaacttct ttatccataa
     4261 tgaagcttat accaaacccg cccgggaaaa taaccggagg agaaatgttt tttggcagcc
     4321 tggatttgct gaaagtagat gaagatacaa tcaggcaaat acggggtaac aatatatcca
     4381 tgatatttca ggagcccatg acttctctga acccggttta tacaattgga gagcaattat
     4441 cggaagtttt ttgtacacat caaaagttgt ccaagaaaga agcaatgaaa aaatcattgg
     4501 atatgttaaa acaggtaaaa attccattgg cagaaaaaag aatgaagcaa tatccccatg
     4561 agttgagcgg gggtatgcgc caaagggtta tgattgcaat ggcacttgcc tgtatgccgg
     4621 agatactgat tgccgatgag cctaccacag ccctggatgt aacaatacaa tcacaaattt
     4681 tacatcttat aagagagatg aaggagaaga tgaatacagc cgttatttta attactcaca
     4741 atatgggcgt agtagctgaa atggctgata atgttatggt tatgtattgc ggaagggcct
     4801 tagagtatgc ggatgtcaga acattattca aagagcccaa gcatccttat accataggtt
     4861 tattgaattc cataccaaga attaaaggcg gaaggaaaaa actatatgcc attaagggct
     4921 cggtacctgt tcccggtgag attacggcag ggtgtcggtt taatagccgc tgtgaatatg
     4981 tatttgagaa atgcctggtt gaagaaccac ctattttcga agttggaaaa agcaaggtca
     5041 ggtgctggaa atatgaaggg aagggtgtga ctgtgtgagt ggaaatatat tagaggtaaa
     5101 gaatttgaag gtccactttc cggtcaaagg agagctcatt cctaaaaatg tcggttatgt
     5161 aaaggcggtg gacggtatca gctttaatat aggctatgcc gagactctcg gattagtagg
     5221 tgaaagcggg tgcgggaaat caacaaccgg tcgtgcagtc ttgaatatgc tgaaggtaga
     5281 ttcgggacaa gttttctttg aaggcaagga tatcctgaag agcagcagga aagaattgaa
     5341 aaagataaga agggatatgc agctgatatt tcaggacccc tttggctcac ttaatcccag
     5401 gatgaccatc ggaagtgcct tagaagaggt cttgtatata caccatatta gggataagaa
     5461 tgagcggcaa aaaagagtaa ttgagctcat tgaaatggta ggtttaaata aaaattattt
     5521 atcgaggtat cctcatgagt tcagcggcgg ccaaaggcaa agggctgtaa ttgcaagagc
     5581 tttggctgta aagccaaaat ttattgtttg tgatgagcct gtgtctgcac tggatgtttc
     5641 cattcaatct caaattatta atttattgga ggagcttcag aaaaacttta agcttacata
     5701 tctgtttatt gctcatgact taagtgttgt caaacatata agcaaccgaa ttgcagtaat
     5761 gtatttaggc aggattgtgg agattgcaga aagcgatgag ttatttaaaa ggccggctca
     5821 tccctataca aaagcactgt tatcgtcaat ccccgtatgt gaccctgact ttcgtaagaa
     5881 tataattctt ttggagggtg atttgcctag tccggctaat cctccaaagg gatgtcgatt
     5941 tcataccaga tgtatttatg ctagggaaga ttgttcaagg aaggaacctg tatctgtcaa
     6001 cctgggaaac ggacatatgg tggaatgtca tttatattca aatgaagagg aggattagtt
     6061 caatgaaaaa aaatatttcg aaaatgtttg cattggtact gattttatcg tgtacagcat
     6121 tagttttttc aggatgttcg aaaaatgatg tgcccgtcgg cgaaacgccg gaaggtaaca
     6181 acggtgagca accggcaaaa gagacaaccc tggttatagc ctacgaaaga gatgcggaaa
     6241 ccctgaatca cataaaaaca ggctggtatt cagattcttt gatttatatg tatgacaggc
     6301 tggtttccag ggactacgat tttaattaca gaccgagttt ggctataagt tgggaaactt
     6361 cggaagatgg aatggtgtgg acatttcatt tgagagacga tgtaaaattt cataacggaa
     6421 agcctttgac tgcacatgat gtaaaattta cttttgacac tattttagac cctgaaactg
     6481 catctccttc tcaaagcgat ttcgcagcga taaagggatt ggaagtaaag gatgaacaca
     6541 cggtagatat aatgctgcat caaccttttc caaatctctt gtttgttttg tcaaatacgg
     6601 cagcaggaat agtaaacaaa gaagcttatg aagagtatgg tgatgagtat ggaattaatt
     6661 atgtagtagg cacaggaccc tatatgttca aagaatggaa acaaggagat aaaattataa
     6721 tagtaaagaa tcccgattac aattggggac ctgaatggat gaccaacagg ggaccggctt
     6781 taatcgacag aatagaattc agaacaatac cggaagaaaa tacgcggctg atggagttag
     6841 aagcaggaaa tgtacatata ttgagggatg tccctgcatc atttttgcaa agagttgaag
     6901 aaattgacga tgtagaagta atgagaagag atgctacaaa acttggatat ttggcttatg
     6961 cctgcgataa ggagcctttt accgacatca gagtaaggcg ggcaataaac catgcaatta
     7021 ataaagaaga tattattaaa tacgttttta gaggatatgg agagcctgct tacggttatt
     7081 tgcctcctgc cctgaaaagc gaatattatg cggacagtaa aaaggatgga tatgagtatg
     7141 atgtggagaa agcaaagcaa ttgttaaagg aagccggcta tggagacggg ctcaagctta
     7201 cattatcggc agaaaattct acggagtaca gccgtttagc ggaagtgctt caagaccagc
     7261 taaaacaggt tggaattgaa acagaaattc aactatatga cagttcaagc tatacgtcaa
     7321 tgttaagaga aggtcaacag gagcttttca taaggcttta cagctggccg aatgccgata
     7381 tattggattg gttcctccta tcgagccagt tcccataccc gaaccattca agatggcagg
     7441 atgaaaagac cgatgagtta attaataaag cagctacaag tcctacatgg gatgaaagat
     7501 ctaaaggata ttttgaagtt caaaagcatt tgattgaaca ggcagtatgg gccccgattt
     7561 atatacctga aaataccatt gcagtaagaa aagaagttaa agatttcaag tttcatcctt
     7621 ggatacctca atataatgac ggtacatatt tggaagttga atagagcagg caaagcttcc
     7681 ggtatatccg gaagctttgc aataattacg attgaggtga attaatgctt aaatatatat
     7741 ttaaaaagct ccttatgatg ataccgattt tgttaggatt gactattatt ttatttttaa
     7801 ttttacattt ggctccggga gacccggtgc atttagtcgt aggacctaac gtgactcctg
     7861 aagtttatga gaatgtcaga aggagcatgg gtttagacag gccgctcata atacaatatt
     7921 ttgattactt gagtaattta gtaaagggag atttaggcag ctcaattttg cagcatagac
     7981 ctgtggcaga tattgttttg gaacgtttcc ctataacaat cggtttagga gtgagggcac
     8041 ttctactttc ctttttaatt gccgttcctg ttggaattat tgcagcagtt aacagaaaca
     8101 agtcagcaga ttttatagct atgacttttg ctttggtcgg catatcaatg cccacatttt
     8161 ggtttggaat aatactgctt tatttctttg catataaatt agggttgttt ccgatatcag
     8221 gttatggtac ctggaaacat ttagtgctgc ccgttattac catggggctc acggatgcag
     8281 cggtaatagc caggatgatg cggtccagca tgttggaagt tattggtcag gactatataa
     8341 ggacggcaag agcaaaaggg ttgagagaaa aagttgtaac aaacaagcat gcgcttaaaa
     8401 atgcattgat acctgtcata accctcttag gcatgcgaat gggctggatt attggaggct
     8461 cggtcacttt ggaaattatt ttcagcatac ccggtttggg aaggacgatg gttgattcaa
     8521 ttttttcaag agactaccct gttgttcaag gctcagtatt aatacttgca acatcggtaa
     8581 tgataagcaa tttaatagca gatattcttt atgcccttgt tgaccccagg ataaggtatt
     8641 aaatctagtt tatataaaag tattaaaaaa gatggagtat aatatggatg acattattat
     8701 agaagacaat ttggaagatg aagtggaaag ttgggagccc aatttttggg aaaggctttt
     8761 ttctaataaa atggctgttt ttggtgctac agtaatatta attttgttta ttaccgctat
     8821 ttttgccccg cttattgcac catatcccta tgatgccatt gacctgcccc agatgttgaa
     8881 agcacctaac tctgaaaata ttttaggaac ggatgaattc gggagggata tattcagccg
     8941 gattgtttac ggctccagaa tttccctaca ggtaggattt attgcggtag gtatttcatt
     9001 ggtaatcggc acaattttgg gagcattggc aggctattac gggggaatta cggactatat
     9061 tatttcagga attaccgata ttgcttattc atttcctata agcctccttg ccattgcctt
     9121 tgttgcggca cttggtcgaa gcttaacaaa tttgattttt gcagtagcac tggttagttg
     9181 gtctggttat gcaagacttg tgcgaggaca gtttatatca ttaaagaacc gtgaatttat
     9241 cgaagcaaca aagatactgg gaatgagtga tgcgcgtata attttcaagc atatgcttcc
     9301 taattcgatg gcacctgtta ttgttcttac tacactcgaa atacctaaag cgattatagt
     9361 ggaatcttct ttgagtttcc tcggtctagg tgtgccgcct ccggccccca gctggggctc
     9421 gattatgagt tcggggcgtg catatattat ggaagcacct tgggtctcta tttttccggg
     9481 attagtgatg gcattggcag tattgggatt caatcttttc ggagatgcat tgagagatac
     9541 gcttgatccg agactgaaag attaagag
//
LOCUS       DAOPUE010000070         9516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_7239_length_9516_cov_8.592538, whole genome shotgun sequence.
ACCESSION   DAOPUE010000070 DAOPUE010000000
VERSION     DAOPUE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7239_length_9516_cov_8.592538"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PKH42_06985"
     CDS             <1..894
                     /locus_tag="PKH42_06985"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOA20214.1"
                     /translation="GTFAGFYLFSHLYSVFMKDDNSIANIISNKVYTAKKIKGDIIVF
                     NILLMSVLAVYTIFIVIQFKYLFSGGILPNGLNYSEYARRGFFELVVLSVLNIALILL
                     TTYLLSDKIYENKNKWALFTKLMLIYLCATTGILLASSYYRMSLYDSAYGFTRLRVLV
                     YIFLFFETVGLIATSVYIIKHNFNILAVYAAVGLTFYLTLNIIKIDEIIAKRNIDMYI
                     SGQAEEIDIDYLMNLSQDALPQIIRLSESDVQIMTRVKTIDFLKDMSAYYAQMENNWQ
                     SYNLTIEKNKDLMKSSNYKWQ"
     gene            910..1167
                     /locus_tag="PKH42_06990"
     CDS             910..1167
                     /locus_tag="PKH42_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019412395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20215.1"
                     /translation="MKKLKFILSDLLLLLGIEIFLSPFILYWFIHGNYERYIWIINGP
                     FPFNCFGGGPFQMLMYVSLFIIGAILIIVSLIIRRKHYGGV"
     gene            1167..1469
                     /locus_tag="PKH42_06995"
     CDS             1167..1469
                     /locus_tag="PKH42_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20216.1"
                     /translation="MIIFLNLGSLIFGLIAWILPVINIFKYKKCEKWFVTSIISISLC
                     AVSIFFQLYYQNYLVKIDDLPALMDTISASSFLSAVLLFVTLILNAINLTIFLLKD"
     gene            1548..2732
                     /locus_tag="PKH42_07000"
     CDS             1548..2732
                     /locus_tag="PKH42_07000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOA20217.1"
                     /translation="MISQEMIKLGTQRSVIRELFSYGLQRAEVVGAENVLDFSIGNPS
                     VPAPKEVQEAIIDIVKNNDPMVYHGYSTSQGHAGCRKAVAGNLNKRFNKSYTLNDIFM
                     TCGAAASLCITFRALTASADDEIIVLAPYFPEYKVFVEGQGAKMKLVQSKNDMRLNLE
                     GIKEAVNKNTKALLINSPNNPSGVVYSKEEIKELSDYLKKMQNELGQTIYLISDEPYR
                     ELVFNDEPVPFIPDYYDNTIICYSWSKSLSLPGERIGYVLVNDKCEDNKTVMAAVGGA
                     ARSLGYVCAPTLFQLVIEKCIDVKPNLEVYRKNRDILYDALTEMGYNVAKPDGAFYLF
                     IEAPNGDSKEFSDKAKEHDVLVVPGTGFGTDTHIRLSYCVDTEKCEKALIVFEKLIKE
                     IK"
     gene            complement(2806..>3836)
                     /locus_tag="PKH42_07005"
     CDS             complement(2806..>3836)
                     /locus_tag="PKH42_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOA20218.1"
                     /translation="LILAGVLFAIIVRINLETILLFFGASEAILPYAMEYMKIIAFGA
                     PFHIVSIGMNHFIRADGNPKLAMYTMFLGAGTNIILDPIFIYGFKMGMAGAALATIIS
                     QFFSFLWVTSYFIGKHSRNKLKIEYLRLKSNLTGKITTLGLPGFSLQLATSALNAILN
                     KNLVFYGGDIAVSGMGVINSIQTLLIMPIIGLNQGVQPIISYNYGAKNYERVKDAAKL
                     AIKAATIIVSFGFIITRLFPVQLISLFNRNTELIKFGKSAIFAWFLCFPVVGFQIVGS
                     NFFQAIGRPKSAMLLTLTRQLILLTPAIIIFPRLWGVEGILYAAPFADFLSFLLTATF
                     FYLGIRKL"
     assembly_gap    3837..3936
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4232..5101
                     /locus_tag="PKH42_07010"
     CDS             4232..5101
                     /locus_tag="PKH42_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOA20219.1"
                     /translation="MKRSDGYLLKDLKPFEKIIPYVMEKRSDAQNFSKQIFPAESIDK
                     YILEKKQSGYSFSYMHVFIAAYVRVIAERPQLNRFVMNNQIYQREKIYVSMAVKRSLR
                     DDGEETTVKFEFTGKETIFEIAEIIDKDIAKALSYSEDNNTDKLAERVMSMPGFVKKL
                     LVRTLKAMDKYNLLPKSIIDASPFHTTMFFTYLKSIDTQYIYHHLYDFGTTGLFAALG
                     KTVKIPIVENDQVVVKKCCEIGYTMDERICDGVYYARSFKLFEKYISNPRLLESELIQ
                     KVPKDQKVKKAFA"
     gene            5172..5873
                     /locus_tag="PKH42_07015"
     CDS             5172..5873
                     /locus_tag="PKH42_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOA20220.1"
                     /translation="MKYTIEKSVFELNPNIKFGILIGNNIRNSETRPEDEERLRRAEA
                     KFRNKVKPDEIRDLINVKLYREVMKNSGINPNKYLPSVEAMFKRIAKGGQLPVINALV
                     DLCNAVSIERGISLGGHDLKDIHDDLEVRYSRKGDVFLPFGAENYEDVEEKELIFTSG
                     NIVQTYKWLWRQSELGKLTLESTDVFFQLVGFDYEEGSSLYDAMSDIEYLVINRFHGT
                     CRKYLVDISNPSIEF"
     gene            5914..7128
                     /locus_tag="PKH42_07020"
     CDS             5914..7128
                     /locus_tag="PKH42_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA20221.1"
                     /translation="MDKKFNYGYVVAVLCFIIAFFYVGSAYNTGSLYVVPVTNHYGFA
                     RAEFSLIFSIISIFMIFASLSFVRLYRRLGIKNLVILGAILCVLAFFVYYKSTNLIQF
                     YIGAVLFGIGITYTNLMTFALLINSWFTENRGTILGLISAGSGFGGSVMSPVLGYLIL
                     TYGFKQAYLLTSIILFVLIIPIALFIKENPVGYATNSEEKLNIKKKTIGQLLKEQSVI
                     IGLTVIFLMGFSMVPWLHIFPSHLIDRGLNELFASKILGGVLFIMALSKILVGAVYDR
                     IGIKFAIGICLVSFVISSVLLLIVDSELIAWFFAALFGISLSSQTVLLPLFISAFAGD
                     EYLSDIIGIASALAGAGIAIGTPIINLTYDLTGSNDMILIAFTILGIFNMLLTIIALK
                     KAKNSKIQETDI"
     gene            7303..>7973
                     /locus_tag="PKH42_07025"
     CDS             7303..>7973
                     /locus_tag="PKH42_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20222.1"
                     /translation="MNKIITIVLVFMLMIVSSCYKFEENPENGEQNNNVNENIENTDE
                     SNENENNIKINADNNNIEKNDEGTDIESISENDDLEIEAEAVIHENNVRIVYPNGDIY
                     EGEMKNGMMDGFGIYTYVNGDVYEGDFKENKPHGHGKLTSPNNFVYEGGMKNGMMDGF
                     GIYTYSNGDVYEGEFKENYQHGHGRFTVPNKYVYEGEFRDDFINGHGVTIYANGDKYE
                     GEFVNG"
     assembly_gap    7974..8073
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8074..9035)
                     /gene="selD"
                     /locus_tag="PKH42_07030"
     CDS             complement(<8074..9035)
                     /gene="selD"
                     /locus_tag="PKH42_07030"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8985..8987),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOA20223.1"
                     /translation="MSSDEIKLTQMTKSAGUAAKIGPDVLAQVLCHLNYDKDENLLVG
                     MSTSDDASVYKISEELALVQTLDFFTPVVDDPYVYGQIAAANSLSDVYAMGGEPKLAL
                     NITCFPLDLPKEVIKSILQGGHDKVKEANALVIGGHTVEDNEPKYGLSVTGFIHPKDV
                     VTNSNAKPGDCLILTKPLGLGILNTGIKAGLVSEEGYKTAVLTMTELNKFSKDAMMKV
                     GVNSCTDITGFGLLGHAYEMAVGSDVTIKFNSSKIPIIEEALSLARMGIIPAGAYKNM
                     EYIKEQVKISKTVAHEIIDCIYDPQTSGGLLISVEKNKSQLLLD"
     gene            complement(9055..>9516)
                     /locus_tag="PKH42_07035"
     CDS             complement(9055..>9516)
                     /locus_tag="PKH42_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOA20224.1"
                     /translation="VGAKYYSELLEYIDFYEDELIFCAPYNKYPSVKNEYLDINTLLS
                     EKFIFRKTGSGTRRLIEQRLSEKGIDIDSLNIISFIDSNEMIKKMIELGLGVSFISKI
                     AVKNEIDLKLVKAFRIEDLNLKRSFYFVHNKNRTLSPLVEAFKDFLTHWKY"
BASE COUNT         3102 a         1395 c         1732 g         3087 t
ORIGIN      
        1 ggaacatttg caggattcta cctgttttca catttatatt cagtttttat gaaagatgat
       61 aacagcattg caaatattat cagtaataaa gtttatactg ccaagaaaat taaaggcgat
      121 ataattgttt ttaatatatt acttatgtca gtattagctg tttatacaat ttttattgtt
      181 atacaattta aatatttgtt ttccggcgga attcttccaa atggtcttaa ctattccgaa
      241 tatgcccgac gcggtttctt tgaacttgtg gttttgagcg tattgaacat agccttaata
      301 ttgttgacca catatttatt gagtgacaaa atttatgaaa ataaaaataa atgggcatta
      361 tttactaagc ttatgcttat atatctttgt gcaacaaccg ggatattgct tgcatcttct
      421 tattacagga tgtctcttta cgacagtgct tacggtttta ccagattaag agtattggtt
      481 tatatattct tgttctttga gacagtaggg ttgatagcaa cttctgttta tattataaag
      541 cataatttta acatattggc tgtttatgct gctgttgggc ttacatttta tttaacttta
      601 aatattataa agatagatga aataattgcc aaaagaaata tagatatgta tataagcggt
      661 caggcagaag aaattgacat cgactattta atgaatttat cacaggatgc actgccgcaa
      721 attataaggc tctctgagag tgatgttcaa ataatgacaa gagttaaaac aatagatttc
      781 ctgaaagata tgagtgcgta ttatgctcaa atggaaaata attggcagag ctacaacttg
      841 acaatcgaaa agaacaaaga tttaatgaaa tctagtaatt ataaatggca gtagcaaagt
      901 gaaattatta tgaaaaagtt gaagtttatt ctatctgatt tgttattatt attaggtatt
      961 gaaatttttc ttagcccctt tattttgtat tggtttattc atggcaatta tgaaaggtat
     1021 atatggataa ttaacggacc tttccctttt aactgctttg ggggaggtcc ctttcaaatg
     1081 ttaatgtatg tttcattatt tataattgga gcaatactga taattgtttc attgattatt
     1141 agaaggaagc attatggagg agtgtaatga taatttttct taatttaggc agtttaatat
     1201 ttggcttaat tgcttggata ctacctgtta ttaatatttt taagtataaa aaatgtgaaa
     1261 agtggtttgt aacttcaatt atcagcatta gtttatgcgc tgtttctata ttctttcaac
     1321 tttactatca aaattattta gtaaaaattg atgatttgcc tgccttaatg gatacaataa
     1381 gcgcttcttc ttttttgtca gcagttcttc tttttgtaac tcttatatta aatgcaatta
     1441 atttaactat atttctactg aaggactgat attttataac atttactttt atctattaat
     1501 atggtaatat agtggaaagg aaatattaca aattattgga ggataatatg atatcacaag
     1561 aaatgattaa attaggcacg caaagatctg ttatcagaga attattttcg tatggtttgc
     1621 agagagcaga agttgtcgga gcagaaaacg ttttggattt cagtatagga aacccaagtg
     1681 taccggctcc caaagaagtg caagaagcca tcattgacat tgtgaagaat aacgacccaa
     1741 tggtttatca cggatacagc acatcgcaag gacatgccgg ctgcaggaaa gcagtggccg
     1801 gaaatttaaa caagagattt aacaaatcct atacattgaa tgatatattt atgacttgcg
     1861 gcgctgcagc ttcattatgt attactttca gagctttgac agcaagtgct gatgatgaaa
     1921 ttattgtctt ggccccttat tttcctgaat ataaagtttt tgttgaagga cagggagcaa
     1981 agatgaaatt ggttcaaagc aaaaatgaca tgagacttaa tttagaagga attaaagaag
     2041 ctgttaataa aaatactaag gcccttctta taaattcacc aaacaatcct tcaggagtag
     2101 tgtattcaaa agaagaaata aaggaattgt cagattattt aaagaaaatg caaaatgaac
     2161 tggggcagac aatctatttg atttccgacg agccctacag agaattggtg tttaatgacg
     2221 agccggttcc ttttattccg gactattatg acaatacaat tatttgttac tcatggtcaa
     2281 aatcattatc attgcccgga gagcgtatag ggtatgtctt agtaaacgat aaatgtgagg
     2341 ataacaaaac tgtaatggct gctgtgggag gagctgcaag gtctcttggt tatgtatgtg
     2401 ctcctacact tttccagctt gttattgaaa aatgtattga tgtcaaacca aatttggaag
     2461 tatacaggaa aaacagagac attctttatg atgcattgac agaaatggga tacaatgttg
     2521 ccaagcctga tggtgcgttc tatttattca tagaagcccc aaatggagat tcaaaagaat
     2581 tctcggataa agcaaaagaa catgatgtct tggtagtacc gggaaccggc tttggaacag
     2641 atacacatat aagactttcg tattgtgtgg ataccgaaaa atgtgaaaaa gcattgattg
     2701 tatttgaaaa gttaattaaa gagattaaat aatataatat aattaagaat ggcgctatgc
     2761 gccattcttc atttctactt cttccatcgt catttacaat tttaattata gttttcttat
     2821 tcctaaataa aagaatgttg ctgttaacaa aaatgataag aaatctgcga aaggtgctgc
     2881 gtatagtatc ccttccacac cccagagtct agggaatatt atgatagcag gcgttaacaa
     2941 aatcaactgc cttgtaaggg ttaataacat tgcagattta ggtctgccta tagcttgaaa
     3001 gaagtttgaa cctactattt gaaagcctac tacagggaaa cataaaaacc atgcaaatat
     3061 tgcagatttc ccaaatttta ttaattccgt attcctgtta aataatgata tcaattgtac
     3121 aggaaacaac cttgtaataa taaatccaaa ggaaacaatt attgtcgctg cctttattgc
     3181 taactttgct gcatccttta ctctttcata atttttggca ccgtagttat agctgataat
     3241 tggctggaca ccttggttta agccaataat cggcattatc agcagagttt gtatgctgtt
     3301 tataactccc attccggaaa cagcaatatc accgccgtaa aatactaaat ttttattaag
     3361 tattgcattt aaagcacttg ttgccaattg aagagaaaac ccgggcagac ctaaggttgt
     3421 aattttacct gtcaaatttg atttaagcct caaatattca atttttaatt tatttctgct
     3481 atgttttccg ataaaatatg aagtaaccca taagaatgag aaaaattgag aaataatagt
     3541 tgccaaagct gctcctgcca ttcccatttt aaaaccataa ataaatattg gatctaaaat
     3601 aatattagtt cctgccccta aaaacatagt atacatggct aatttcggat ttccgtcagc
     3661 tcgtataaaa tggttcatac caatacttac tatgtgaaag ggtgctccga atgcaattat
     3721 tttcatgtat tccatagcat aaggaaggat tgcttcgctg gcaccaaaga aaagcaatat
     3781 agtttctaag ttaatacgca ctattattgc gaagagtact cctgccaaaa taagcannnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatag caggtattga aaacttaaat
     3961 aaaagctttg atattttttc tgtgcctaat ggatttatgt agtccaattt tgcctccgtt
     4021 tatgttatta atagtgatat tatactataa atttttatat attgaaacaa gaagatttga
     4081 gcaataaaaa ataaaacaat tgataataat gcaaatttgt gttattcttc gattaagcgg
     4141 tgttccggaa aatgtttttt tccggtacgc accgctatgt attatatgag ctccttgaat
     4201 tgcagaattt agattagtat atggaggatg tatgaaaaga agtgacggat atttattaaa
     4261 agacttgaaa cctttcgaga aaataatacc ttatgtcatg gaaaaaagaa gcgatgctca
     4321 aaatttttct aagcagattt ttccggcgga aagtattgat aaatatattt tagaaaaaaa
     4381 gcaaagcggt tatagtttta gttacatgca tgttttcata gccgcttacg taagagtgat
     4441 tgcagaaaga ccgcagctta accgttttgt aatgaacaat caaatttatc agagagaaaa
     4501 aatttatgtt tcaatggctg taaaacgttc acttagagat gacggggaag aaacaaccgt
     4561 aaaatttgaa tttaccggaa aagaaactat ttttgaaatt gctgaaatta tagataaaga
     4621 cattgctaag gcattatcct acagtgaaga taataatacc gacaagcttg ctgaaagagt
     4681 aatgtcgatg cccgggtttg tgaagaaatt attggttaga actctaaaag cgatggataa
     4741 gtataactta cttccaaaaa gtattattga tgccagcccc tttcatacca caatgttttt
     4801 tacttactta aagtcaatag atactcagta tatctatcat catctttatg attttggaac
     4861 aacaggctta tttgctgcat taggcaagac agtgaagatt ccgatagtag agaatgacca
     4921 ggttgttgtt aaaaaatgct gcgaaatagg ctatacaatg gatgaaagaa tatgtgacgg
     4981 tgtgtattat gcaaggtcct ttaagctctt tgagaaatat atcagcaatc ctagattatt
     5041 ggaatcagag cttattcaaa aagttccaaa agaccaaaaa gttaaaaagg catttgctta
     5101 ataagaatcc ggacgtggaa aaatagcagt ctctatggta gagaggcaga ataactaaga
     5161 ggagttaaaa aatgaaatat acaattgaaa agtctgtgtt tgaattaaat cctaatataa
     5221 aattcggaat attaataggc aataacatta gaaattcaga gacaaggcct gaggatgaag
     5281 aaaggctgag aagagcagaa gccaaattca gaaataaagt taagcctgat gaaattaggg
     5341 atttgataaa tgtgaagctg tacagagaag ttatgaaaaa ttcaggtatt aatccaaata
     5401 aatatcttcc ttccgtagaa gcaatgttca aaagaatagc aaaaggcgga cagctgccgg
     5461 ttataaatgc cttagtcgat ttatgcaatg cagtatccat agaaagagga atatccttag
     5521 gcgggcatga tttaaaagat attcatgacg atttagaggt gcgttacagc agaaagggag
     5581 atgtgttttt acctttcggg gctgaaaact atgaggatgt tgaagaaaag gagctgatat
     5641 tcacaagcgg caacatagtc cagacatata aatggctttg gaggcaaagc gaattaggaa
     5701 agcttactct tgaaagcact gacgtatttt tccagcttgt agggtttgat tatgaagaag
     5761 gctcatccct ttatgacgct atgagcgata tagaatattt agtaattaac aggtttcatg
     5821 gtacctgcag gaaatatctt gtagatataa gcaatccatc catagaattt taaatcttca
     5881 atttaggcag catttttttg caaaggagtt tttatggaca aaaaatttaa ctatggatat
     5941 gttgtagcag tattatgctt tattattgca tttttttatg ttgggagtgc ttataataca
     6001 ggcagtctgt atgttgttcc ggtaaccaat cactatggtt ttgccagagc tgaattctcg
     6061 ttgattttca gtattatatc aattttcatg atttttgcca gtctttcttt tgtgagatta
     6121 tacagacgct taggtatcaa aaacttagtt atcttgggag ccattttatg tgttttggct
     6181 ttctttgttt attacaaatc gactaacctt attcaatttt atataggcgc cgtcttgttt
     6241 ggaataggaa taacgtatac aaatttgatg acctttgccc tgcttataaa tagctggttc
     6301 actgaaaaca gggggacgat cttaggtttg atttctgcag gaagcggatt tggcgggtct
     6361 gttatgagtc ctgtactagg ttaccttata ttgacttacg gctttaaaca agcttattta
     6421 ttaacttcga ttattttgtt tgttttgatt attccgattg ctttatttat caaagaaaac
     6481 cctgtcggat atgcaacaaa tagtgaagaa aaattaaata ttaagaaaaa aacaatcggg
     6541 cagttattaa aagagcaaag tgtaataata ggtcttaccg ttatattttt aatgggtttc
     6601 tcaatggtgc catggcttca tatttttccg tctcatttaa tagacagggg attaaatgaa
     6661 ttatttgctt ctaaaatatt aggcggagtt ttatttatta tggcattgtc aaagatttta
     6721 gtcggtgctg tttatgacag aattggaatt aagtttgcta tcggtatttg cttagtatct
     6781 tttgtaataa gctcggttct tttgcttatt gttgacagtg agttaatagc gtggtttttt
     6841 gctgcattat ttggcatttc attaagttct caaacagtgt tattaccctt gttcatttca
     6901 gcttttgcag gggatgaata tttaagtgat attatcggga tagcatctgc tttggctgga
     6961 gcaggtatag ctattggaac tcctataatt aatcttacat atgatttaac tggttcaaac
     7021 gatatgattt taattgcatt cacaatcctt ggtattttta acatgctgct tactattatt
     7081 gcattaaaaa aagcaaagaa ttccaagatt caggagacag acatttgaat tcagtataaa
     7141 atccttatca ctattcttta ccatgcatca taaggcatgg ttttttttga tgaaaatgaa
     7201 acgctagcat aatttaacag taatgagaat tagtgctatt attaatttaa ttattgacgg
     7261 taataatata tattataaag gggttggtat tggaggcggg ttatgaataa gattattacc
     7321 attgtattag tttttatgtt aatgatagtg tcatcatgct ataaatttga agaaaatcct
     7381 gagaatgggg agcaaaataa caatgtaaac gagaatattg aaaacactga tgaaagcaac
     7441 gaaaatgaaa acaatataaa aataaatgct gataataaca atattgaaaa aaatgatgaa
     7501 ggaactgata ttgaaagtat tagtgaaaat gatgatttag aaattgaagc ggaagcagta
     7561 attcacgaaa ataatgttag aatagtatat ccgaacggcg atatttacga aggtgaaatg
     7621 aagaatggaa tgatggacgg attcggcatt tatacctatg taaacggtga tgtttatgaa
     7681 ggagatttta aggaaaataa acctcatggt catggaaaat taacatcacc aaataatttt
     7741 gtatatgaag gcggaatgaa gaacggaatg atggacggat tcggcattta tacatattct
     7801 aacggggatg tttatgaggg agagtttaaa gaaaattacc agcatggaca cggcagattt
     7861 accgtgccga ataaatatgt atatgaagga gaatttagag atgattttat aaacggtcat
     7921 ggagtaacca tatatgcaaa cggcgataaa tatgaaggtg aatttgttaa cggnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcatcaa gaagtaattg agacttgttt
     8101 ttttctaccg atatcaaaag tcctcccgat gtttgcgggt cataaatgca gtcgattatt
     8161 tcatgagcta ccgtcttaga aattttaact tgctccttaa tatattccat atttttataa
     8221 gctccggcag ggataatacc cattcttgcc aaggataagg cttcttcaat aatcggtatt
     8281 ttagaagaat taaatttaat ggtaacatcg ctacccacag ccatttcata ggcatgaccg
     8341 agcaaaccga atccggttat gtctgtacag ctgtttacac cgactttcat catagcatcc
     8401 ttagaaaatt tgtttaattc agtcatggta aggacagcgg ttttgtatcc ttcttcactt
     8461 actaagccgg cctttatgcc tgtatttaaa atccccaatc ccaaaggctt ggtaagaatc
     8521 agacaatctc ccggcttggc attgctgttg gtaaccacat ccttgggatg tataaatcca
     8581 gttacggata atccgtattt cggctcatta tcttccactg tatggccgcc gataaccagg
     8641 gcattagctt ccttgacttt atcatgccca ccttgaagaa tactttttat aacttcctta
     8701 ggtaagtcca ggggaaagca ggtgatattt aaagctaact tgggctcgcc gcccattgca
     8761 tatacatcgc tcagtgaatt tgcagcagct atttggccgt acacataggg gtcatcaaca
     8821 accggtgtaa agaaatccaa ggtttgcacc aaggcaagct cctcgcttat cttgtaaacc
     8881 gaagcatcat ccgaagtact cattcccaca agaaggtttt catctttatc atagtttaga
     8941 tggcacaata cttgtgccag gacatcaggt ccaattttag ctgctcaccc ggcactttta
     9001 gtcatttgag ttaattttat ttcatctgaa gacatatttt cacttcctcg tattttagta
     9061 tttccaatgc gttaaaaaat ccttaaatgc ttctaccaaa ggggataggg ttcgattttt
     9121 gttgtgaaca aaatagaagc ttcgttttaa attcaagtct tctattctaa aggctttaac
     9181 cagtttcaaa tcaatttcgt ttttaactgc tatttttgaa ataaaactga ctcccaatcc
     9241 aagttcaatc atttttttaa tcatttcatt gctgtcgatg aaagaaatga tgtttaaact
     9301 atctatatca ataccttttt ccgaaagtct ctgctcaatt aatcttctgg tacccgaccc
     9361 tgtttttctg aaaataaatt tttccgaaag caaggtatta atatccaagt attcattttt
     9421 aacggaaggg tatttattgt aaggtgcgca aaaaataagc tcatcttcat aaaaatctat
     9481 atattccaat aattctgaat aatactttgc tcctac
//
LOCUS       DAOPUE010000071         8896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_7877_length_8896_cov_9.021378, whole genome shotgun sequence.
ACCESSION   DAOPUE010000071 DAOPUE010000000
VERSION     DAOPUE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_8896_cov_9.021378"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1597
                     /gene="thrC"
                     /locus_tag="PKH42_07040"
     CDS             107..1597
                     /gene="thrC"
                     /locus_tag="PKH42_07040"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005968097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOA20225.1"
                     /translation="MAKSYESTRNFELKAAASEAVLKGIAEDGGLFVMRNLGERKVNI
                     HSLVGKNYVEIAEVILEILLDDFSKDKLKECIRKAYTDKFSAEEITPVVKVGQNYVTE
                     LFHGPTSAFKDVALSILPHLMTAAYEMNGINSEIIILTATSGDTGKAALEGFKDVKGT
                     RIIVFYPEGGVSQIQETQMITQEGNNTFVCAIEGNFDDAQTGVKKIFANENFIKEMAA
                     KNKILSSANSINIGRLVPQIVYYFVSYMKLVEKGAIKLGDKVNFAVPTGNFGNILAGY
                     YAKILGLPVNKLICASNENNVLYDFIRRGVYDKNRDFHKTISPSMDILVSSNLERLLY
                     YLSGKNNQIVKEYMKKLNTSGIYEISDEMAEKLREDFCGGCVFKEDAQKTIKDTFKKY
                     GYLLDTHTAVAYKALEDYKTETEDDTVSVVLSTASPFKFAKSVYSSLFGETDDDEFVL
                     MEELSEKTKIKIPENLRVLQYKKLRHFEVCSVDEMEDFVISALNEY"
     gene            1609..2499
                     /gene="thrB"
                     /locus_tag="PKH42_07045"
     CDS             1609..2499
                     /gene="thrB"
                     /locus_tag="PKH42_07045"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOA20226.1"
                     /translation="MVRVTVPATTANIGPGFDVLGLAFKLYNRITFERIEKGLIIKGC
                     PEVYNNNENLVYKSFVVTAENLGKKVDGLKISIDAKIPVSRGLGSSSACIVGGVYGAN
                     TFYKGGLTKDEMFKIAAAIEGHPDNISPCVYGGLTASMIEKERPYAVGYNISSTLKFY
                     ALIPDFETSTSEARKILPKTIDFKDAVFNVSRVAVLLKALETGDMDMINISLDDRLHQ
                     IYRGTLIHEYDNIREICAEAGSPALFISGAGPTLMNITKDEKFPEKIKDSISKLKHKW
                     RIEMLEPDKSGATVEVDSEK"
     assembly_gap    2523..2622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2623..3057
                     /locus_tag="PKH42_07050"
     CDS             <2623..3057
                     /locus_tag="PKH42_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOA20227.1"
                     /translation="MKKYLIVNKKILPEVYEKVIEARNLINTGSVKGISEAVKVVGIS
                     RSTYYKYKDYVFSPDENQIGRKAVISMMLKHEKGILSNVLNYLSQENANILTINQSIP
                     INNKASVNVSLDISDLSKSIDEVIADIKKIRGATQVKLLSVE"
     gene            3067..4275
                     /locus_tag="PKH42_07055"
     CDS             3067..4275
                     /locus_tag="PKH42_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOA20228.1"
                     /translation="MFGLVLEGGGARGAYHVGAYKAIKELNIEIGGIAGTSIGAINGA
                     MMVQGDYELLEKIWLSVDSYELFDLDEKTIEDLRNFNLRNINFSYLLHQSKEILSNRG
                     LDTSRIRELFDYYIDEEKIRSSNIDFGIVTVNLTDKKPMELMKEDIPEGKLIDFLIAS
                     ANLPAFRIEEMDGKKYLDGGFYNNLPIDILANKGYKNIIAVRTLAIGIIRKAKIKDLN
                     IIFIQPVESLGSMLGALDFNRERAEEYINLGYYDAMKVFKKLKGFKYYCIPFEGNFIN
                     ILTDFYNKYKERLCNIGRFLGYEDMCEDRMFFEKIIPRLESILDMKGKNDYQDICIRF
                     FERIAEKYGVERFKIYKAEEFVELTIEKFRENPTAFIKNVPNFIKQNRILSLAVKDDL
                     IVEIFAELFL"
     gene            4517..5929
                     /gene="cysS"
                     /locus_tag="PKH42_07060"
     CDS             4517..5929
                     /gene="cysS"
                     /locus_tag="PKH42_07060"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOA20229.1"
                     /translation="MKIYNTLSRTIEDFKPIDENLVKMYTCGPTVYNYAHLGNLRMYI
                     HEDVFEKTLRYLGYNVKRVMNITDVGHLESDADEGEDKMLKGAKRENKTVWEIAQYYT
                     DAFFDDIDRLNIKRPDVVAKATDYIDEYIEFIKVLEEKGYTYFANGNVYFDITKVKDY
                     TKLSGMDLDSLRSATREGVDLDVNKKNPHDFVLWFTKSKFENQAMKWDSPWGVGYPGW
                     HIECSVISLCNLGEEMDIHCGGVDHIPVHHTNEIAQTESYTGKDWVKYWWHGEFLIDN
                     EGKMSKSSGEFLTLELLIKKGYNPLAYRYFVLNSHYRKQLAFSFDSIASAENAYNKLK
                     SRISSIKNNADGIKENHEAYNKFKNDFENCLRDDLNTANAITVLYEMLKTDTLNNYEK
                     LSLIEDFDKVLSLDLLKEEEKDEVHDELSAYIEEMIQKRQEAKKNKDYQLADQIRAEL
                     LEKGIMLEDTRQGVNWKKIN"
     gene            5968..6363
                     /locus_tag="PKH42_07065"
     CDS             5968..6363
                     /locus_tag="PKH42_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOA20230.1"
                     /translation="MNKTYMYSPAQLAYAGDAVYELLVRSYVLKNHDLSVNKMHRLTV
                     RFVKADAQAYIISKLNDELTDEEKKIVKRGRNAKVTSSPKNAEFMDYRYATGFEALLG
                     YLFLNNDIERLMYIFEKVVEIIIEKDDSN"
     gene            6360..7088
                     /gene="rlmB"
                     /locus_tag="PKH42_07070"
     CDS             6360..7088
                     /gene="rlmB"
                     /locus_tag="PKH42_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOA20231.1"
                     /translation="MRIIEGRNPVIEALRSDAEIDTILINKDAAQGSLNKIFELAKKK
                     NILIKNVDRALLDRLSENKKHQGVIAEAMEYKYKDIDDIFNYAKEKNEKPFIVILDEI
                     TDVHNLGSIIRSAECLGAHGVIIPKRRAAQINSVVAKSSAGAIEYLPVCRVTNISDTL
                     ETLKEKGLWIYGADMEGEKYIYEENFDVPVGLVIGSEGSGLGRLVKEKCDILIKIPMK
                     GNINSLNASNAASIIIYEIMKQRT"
     gene            7091..7609
                     /locus_tag="PKH42_07075"
     CDS             7091..7609
                     /locus_tag="PKH42_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOA20232.1"
                     /translation="MARTKKEYLFIDGYNIINQWQYYRDISRNIENARNKLIELLVEY
                     QAYKGLKVVVVFDAHLVKGSREKKEYVSGVEVVYTKEHETADSYIEKQLDKIGRYEKV
                     RVATSDRAIQQIVLSRGGTRLTAQELILELENTKKDIRIKTENPSQKGANIDRILDTE
                     TLNKLDKIRKKI"
     gene            7710..8360
                     /locus_tag="PKH42_07080"
     CDS             7710..8360
                     /locus_tag="PKH42_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20233.1"
                     /translation="MINSRAIYGNLRYGQEYDLFTDRELIKKFNVGNIEAEKCLMKRY
                     FYLIRRIVNSFNIEDRRKDDILQESMIGLFKAMKTYNERYDVSFKTYAEICIRRQIIS
                     ALRKSEKYETVNIFTVFNCFESDSEYDFVEQMYDDSYNPENILMYEEEKKYYIEFASQ
                     YLSDYENSVLKEYFNGKTYKEIAEILNTNLKSVDNALQRVKKKTFDKWALFTTKAI"
     gene            8511..8586
                     /locus_tag="PKH42_07085"
     tRNA            8511..8586
                     /locus_tag="PKH42_07085"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8544..8546,aa:Thr,seq:ggt)
     gene            8621..>8896
                     /locus_tag="PKH42_07090"
     CDS             8621..>8896
                     /locus_tag="PKH42_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOA20234.1"
                     /translation="MAKQKFERTKPHVNIGTIGHVDHGKTTLTAAITLVLNRRYGTGE
                     FIAFDHIDKAPEERERGITISTSHVEYETPNRHYAHVDCPGHADYVKN"
BASE COUNT         3241 a         1186 c         1859 g         2510 t
ORIGIN      
        1 gctcaggatg acatcaagga cttctcattg tcatatttaa aaaaacactt gtaattatat
       61 cgggaacagt atatcatata ttattatata tgaggacgga gggaatatgg caaagagtta
      121 tgaaagcacc agaaattttg aattgaaagc tgctgcctcg gaagccgtat taaagggtat
      181 tgcagaggat ggcggattat tcgtaatgag aaatttaggt gaaagaaaag taaatataca
      241 tagtctagtc ggtaaaaatt atgtggaaat tgctgaagtt attctcgaaa ttttgctcga
      301 tgatttttca aaggataaac ttaaggaatg tataagaaaa gcgtatacgg ataaattcag
      361 cgcagaagaa ataactcctg ttgtaaaggt gggacaaaat tatgtgacag agcttttcca
      421 tggacctact tcggcattta aggatgtggc tctttcaata ttaccgcatc taatgacggc
      481 tgcttatgaa atgaacggca taaacagcga gataataata cttaccgcaa cttcagggga
      541 tacgggtaaa gctgcattgg aaggatttaa ggatgttaaa ggaacgagaa ttattgtatt
      601 ttatcccgaa ggcggggtca gtcagattca agaaacacaa atgataactc aggaggggaa
      661 taatacattt gtttgtgcaa ttgaaggaaa ttttgatgat gctcagaccg gggttaagaa
      721 aatttttgct aatgaaaatt tcattaagga aatggctgca aaaaataaaa tattatcatc
      781 tgcaaattcc atcaatatag gaagattggt tcctcagata gtttattatt tcgtttccta
      841 tatgaagttg gttgaaaagg gtgctatcaa actaggagac aaggttaatt ttgcagtacc
      901 taccggaaat tttggaaata ttttggcagg atattatgca aagatattgg gacttcccgt
      961 aaataagctt atttgtgctt caaatgaaaa caatgtgctg tatgatttta tcagaagggg
     1021 agtttatgac aaaaacagag attttcataa aaccatttca ccatccatgg atattttggt
     1081 atcaagcaat cttgaaaggc tcttgtatta tttgagcggc aaaaacaatc aaatagtcaa
     1141 agaatacatg aaaaagctaa atacttcagg tatttatgaa atatccgatg aaatggctga
     1201 aaaactgaga gaggatttct gcggaggatg tgtttttaag gaagatgctc aaaaaaccat
     1261 caaggatacc tttaaaaaat acggatatct ccttgatacc catacagcgg tggcatataa
     1321 ggcgcttgag gattataaaa cagaaactga ggatgatact gtaagcgtag ttctttcaac
     1381 tgcaagccca tttaaatttg ccaaaagtgt ttacagctca ctttttggag agacggatga
     1441 tgatgaattt gtattaatgg aagaattgtc cgaaaaaact aaaataaaaa tacctgaaaa
     1501 cctaagagta cttcaatata aaaaattaag gcattttgag gtttgcagtg ttgatgaaat
     1561 ggaagacttt gtgatttctg ctttaaatga atattaatga ggtgaaaaat ggttagagta
     1621 actgttccgg caactactgc caatatagga cccggctttg atgtcttggg acttgcattt
     1681 aaactgtata acagaattac ttttgagaga attgaaaagg gactgataat taaaggttgc
     1741 ccggaagttt ataataataa tgaaaattta gtttataaat cgtttgttgt tactgctgaa
     1801 aatttaggga aaaaggttga tggacttaaa atttccatag atgccaaaat tcctgtttca
     1861 aggggtttag gaagcagctc ggcatgtatt gtgggagggg tttatggtgc taacactttt
     1921 tacaagggag gattgacaaa agatgaaatg tttaaaattg ccgctgcgat tgaaggacat
     1981 cccgataata tttctccctg tgtttatgga ggactgacag catccatgat tgaaaaagaa
     2041 agaccttacg ccgtcggata caatataagc agtacactaa agttttatgc cttaattcct
     2101 gattttgaaa cttcgacaag tgaagcaaga aaaattctcc ctaaaacaat tgacttcaag
     2161 gatgcggtat ttaatgtatc aagggttgca gtattactaa aggcattgga aacaggcgat
     2221 atggatatga ttaacatttc attggacgat agattgcatc agatttacag aggcacttta
     2281 attcatgaat acgataatat aagagaaatt tgtgctgaag ctggctcgcc ggcattgttt
     2341 ataagcggcg caggaccgac tttgatgaat attacaaaag atgagaaatt tccggaaaaa
     2401 attaaagaca gcattagcaa attaaaacat aaatggagaa ttgaaatgct ggaacccgat
     2461 aaaagcggag caacagtaga agtcgactct gaaaaatagg gttagggcat ctttcgagct
     2521 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatgaaaaa gtatttgatt
     2641 gtcaataaaa aaattcttcc ggaagtatat gaaaaggtaa ttgaagcaag aaatttaatc
     2701 aacaccggaa gtgtgaaagg tatcagcgag gcagttaagg ttgttggtat aagcaggagt
     2761 acatattata agtacaagga ttatgttttt tctccggacg aaaatcaaat aggcagaaaa
     2821 gcagtaattt cgatgatgct aaagcatgaa aagggaatac tctcaaatgt tttgaattat
     2881 ttgtctcagg aaaatgcaaa tattcttacc ataaatcaaa gcattccaat taacaacaag
     2941 gcatctgtaa atgtatcatt ggatatttca gatttaagca agtctattga cgaagtaata
     3001 gccgatataa aaaaaataag gggcgcaact caagtaaaat tattatcagt tgaatagggt
     3061 gatattatgt tcggattggt tttagaagga ggaggcgcaa ggggagccta ccatgtagga
     3121 gcctataaag ctataaaaga attaaatata gaaatcggag gaatcgccgg aacatccata
     3181 ggggctatta atggggcaat gatggtccaa ggggactatg aattgctgga aaagatatgg
     3241 ttaagcgttg attcctatga attgtttgat cttgacgaga agacaattga agatttgagg
     3301 aactttaatt tacgaaatat taatttttcc tatcttttgc atcaatctaa agaaatacta
     3361 agcaatagag ggttggacac ttcaagaata agagaattgt ttgactatta tattgatgag
     3421 gaaaaaatcc gcagttctaa tattgatttt ggaatagtta ctgttaatct gaccgacaag
     3481 aagccgatgg agcttatgaa ggaggatata ccggagggaa aattaattga ctttttaata
     3541 gcaagcgcaa accttcctgc ttttagaata gaggaaatgg acgggaaaaa atatttagac
     3601 ggagggtttt ataacaatct tcctatagat attctagcca acaaaggata taaaaatatt
     3661 atcgccgtaa gaacactggc aattggtatt atcagaaagg caaaaataaa agacttaaat
     3721 attatcttta ttcaaccggt tgaaagctta ggctcaatgc tcggcgcttt agattttaac
     3781 agggaaagag ctgaggaata cattaattta ggctattatg acgccatgaa ggtttttaaa
     3841 aagctaaaag gctttaaata ttactgtatt ccttttgagg gtaattttat aaatatattg
     3901 acggattttt ataataaata caaagaaaga ttatgtaata tcggacgttt tttgggctat
     3961 gaagatatgt gcgaagacag aatgtttttt gaaaaaatta tacccagatt agaatcaata
     4021 ttggatatga agggtaaaaa tgactatcag gacatatgca ttaggttttt tgaaagaatt
     4081 gcagaaaagt atggtgttga aagatttaaa atatataaag ctgaagaatt tgttgaattg
     4141 acaatcgaaa aattcagaga aaatcctact gcttttatta aaaatgtacc gaattttatt
     4201 aagcaaaaca gaattttgtc attagcagtt aaggatgatt tgattgtgga aatttttgca
     4261 gagttattcc tttagtggta tggggaaaca cttggtgcca ggcacctcat gaaggtgcct
     4321 ggcaccgtcg catcaacggt taacgggggt gctgtcacaa aagatgacag cacccccgtc
     4381 cccttgtcat tgtgctattt ttcgacaatt tttgcattaa agttacttta tcttaacaaa
     4441 aaatattggc aaattatgct tgatactgtt tcaaaggtaa tatataataa gtttaagtga
     4501 agaggagcgg atttacatga aaatctataa cacattatcc agaacaatag aagattttaa
     4561 accaattgat gaaaacttag taaaaatgta cacttgcgga cctaccgtat acaactatgc
     4621 acatttgggc aatttaagaa tgtacattca cgaggatgtt tttgagaaaa ccctcagata
     4681 cttaggttac aatgttaaaa gggtaatgaa cattaccgat gtaggtcact tggaatcaga
     4741 tgccgatgaa ggcgaagaca aaatgctgaa gggtgcaaaa agagaaaata aaaccgtttg
     4801 ggaaattgca caatattata ctgatgcctt ttttgacgat atagacagac ttaatattaa
     4861 aagacctgat gttgttgcaa aagctactga ctacatagac gaatatattg agtttatcaa
     4921 agttttggaa gaaaagggct acacatattt cgcaaacggc aatgtgtatt tcgacataac
     4981 caaggttaag gattatacaa agctttccgg aatggatttg gacagtttaa gaagcgccac
     5041 aagggaaggg gttgacttag atgttaacaa gaaaaaccct catgactttg ttttgtggtt
     5101 tacaaaatcc aaatttgaaa atcaggcaat gaaatgggat tctccatggg gagtaggtta
     5161 tcccggatgg catatagagt gttccgttat cagcctctgc aatttagggg aagaaatgga
     5221 tattcactgc ggaggggtag accatattcc ggttcatcat accaatgaaa tagctcagac
     5281 agagagctat accggcaagg attgggttaa atactggtgg cacggtgaat tcctcatcga
     5341 caatgaagga aagatgagta agtcaagcgg tgaatttttg acccttgaac tccttattaa
     5401 gaaaggttac aatcctttgg catacaggta ttttgtttta aattcccatt acagaaagca
     5461 gcttgctttt tcgtttgatt ctattgcttc tgctgaaaat gcatataata agctgaaaag
     5521 cagaatcagc tccattaaaa ataatgcgga tggaataaaa gaaaatcatg aagcttataa
     5581 taaattcaaa aatgattttg aaaattgttt aagggatgat ttaaataccg caaatgcaat
     5641 aaccgtttta tatgaaatgc ttaaaacgga tacattaaac aattatgaaa agttaagcct
     5701 tatagaagat tttgataagg tcttgtcatt ggatttgtta aaagaagaag aaaaagatga
     5761 ggttcatgat gagctgtctg catatatcga ggaaatgatt caaaaaaggc aggaagcaaa
     5821 gaaaaataaa gactatcagc tggctgacca aatcagggca gaactcttgg aaaagggtat
     5881 tatgcttgaa gataccaggc agggtgtaaa ttggaagaaa ataaattaac caaagaattt
     5941 gaagttctta ataaaataag cgataaaatg aacaaaacat atatgtattc acctgcacaa
     6001 ctggcatatg caggtgatgc tgtttatgaa ttgctggtaa gaagctatgt tttaaaaaat
     6061 catgacttaa gcgtaaataa aatgcacagg cttaccgtca ggtttgtaaa ggctgatgct
     6121 caggcttata ttataagcaa attaaatgat gaactgaccg atgaagagaa gaaaatcgta
     6181 aagagaggaa gaaatgccaa ggttacttct tctcctaaaa atgctgaatt tatggattac
     6241 agatatgcta cgggatttga agctttgctt gggtatctgt ttttaaataa tgatattgaa
     6301 agacttatgt atatatttga aaaagttgtt gagataataa tagaaaagga tgacagtaat
     6361 tgaggataat cgaaggaagg aatccggtaa ttgaagcatt gagaagcgat gccgaaattg
     6421 ataccatact gataaataag gatgcggctc aaggctcttt aaataaaatt ttcgagctgg
     6481 ctaaaaaaaa gaatatattg attaaaaatg ttgatagggc acttttggac agattaagcg
     6541 agaacaaaaa gcatcaggga gtcatagcag aagctatgga atacaaatat aaggatattg
     6601 acgatatatt taattatgca aaagaaaaaa acgaaaagcc ttttatagtg attcttgatg
     6661 aaattaccga tgtccataat ttaggctcta ttattcgctc ggccgaatgc ttaggcgccc
     6721 acggtgttat aattcccaaa agaagagctg ctcaaataaa tagtgttgtt gcaaaatctt
     6781 ccgccggtgc aattgaatat ctacctgtct gcagggttac caatataagt gacacattgg
     6841 aaacattaaa ggaaaaggga ctttggatat acggagcaga catggaaggt gaaaaataca
     6901 tctatgaaga gaattttgat gttccggtag ggttggttat aggaagcgag ggttcaggct
     6961 taggaagact ggttaaagaa aaatgtgata ttttgattaa aatccccatg aagggaaata
     7021 taaattcttt aaatgcttcc aatgcagctt caattattat atatgaaata atgaagcaaa
     7081 ggacataaaa atggctagaa caaagaagga atatcttttt atagatggat acaatattat
     7141 aaatcagtgg cagtattaca gggatatttc aagaaatatc gaaaacgcaa ggaataagct
     7201 tattgagctt ctggttgaat atcaagcata caaaggactg aaggtagttg ttgtatttga
     7261 tgctcacttg gtaaaaggca gccgcgaaaa gaaagagtat gtttcaggag tggaagttgt
     7321 ctatactaag gagcatgaaa cagcagacag ctatattgaa aagcagcttg ataagatagg
     7381 aaggtatgaa aaagtccggg ttgccacgtc agacagagca attcaacaaa ttgttttgtc
     7441 aagaggaggg acaaggctca ctgctcaaga gctgattttg gaattggaaa acactaaaaa
     7501 ggatattagg ataaaaaccg aaaacccgag tcaaaaagga gctaatatcg ataggatact
     7561 ggataccgaa actttgaata aacttgataa gattagaaaa aaaatttagc actttgacaa
     7621 caattcatga tttctcaata tgtatatcct ttgattataa cagtatataa tttattatta
     7681 ttttatatgt tatgaattgg aggaacttta tgattaattc tcgcgctata tacggtaatc
     7741 taagatatgg gcaagaatat gacttattta cggatagaga actcataaaa aaattcaatg
     7801 taggaaatat agaagctgaa aaatgcttaa tgaaaagata tttttatttg attagaagaa
     7861 ttgtcaactc ttttaatata gaagacagaa gaaaagatga tattttgcag gaatcaatga
     7921 ttgggctttt taaagcgatg aaaacttata atgagaggta tgatgtaagc tttaaaacat
     7981 atgctgaaat atgcataaga agacaaatta tttcagcact gcgaaagtca gagaaatatg
     8041 aaacagttaa tatatttact gtatttaatt gttttgaaag tgacagtgaa tatgattttg
     8101 tagagcaaat gtatgatgat tcttataatc ctgaaaacat attgatgtat gaggaagaaa
     8161 aaaaatacta tattgaattt gcatcacaat atttaagcga ttatgaaaac tccgtattaa
     8221 aggaatattt taacggaaag acatataaag aaattgctga gatattaaat actaatttaa
     8281 aatcagtaga taatgcattg caaagggtta agaaaaaaac attcgataaa tgggcattgt
     8341 ttacaacaaa agcaatatga cacaatacat aatatgagat tcttcgcagc ctgcactcaa
     8401 aacgacaaag tcatcgggtg gggtgaggag aatctcttaa attttcctat tgatttattt
     8461 tccggttgtt gatataatga aacttgttaa caacaagggt ttgacagcat gcttgcgtag
     8521 ctcaggaggt agagcacctc attggtaatg aggaggtcgg cagttcgagt ctgctcgtaa
     8581 gctccaaaaa aatttaaaaa ataatccggg agggattgaa atggcaaagc aaaaatttga
     8641 aagaacgaaa ccgcatgtta atataggtac tatcggtcac gttgaccacg gaaaaacaac
     8701 attaacggcg gcaattacgt tagtattgaa cagaagatac ggaaccggag aatttatagc
     8761 atttgatcat attgataaag ctccggaaga aagggaaaga ggaattacaa tatcaacatc
     8821 tcacgttgaa tatgaaactc caaacagaca ctacgcacac gttgactgcc cgggtcacgc
     8881 tgactatgtt aagaac
//
LOCUS       DAOPUE010000072         8763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8032_length_8763_cov_9.368856, whole genome shotgun sequence.
ACCESSION   DAOPUE010000072 DAOPUE010000000
VERSION     DAOPUE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8032_length_8763_cov_9.368856"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="PKH42_07095"
     CDS             <1..125
                     /locus_tag="PKH42_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HOA20235.1"
                     /translation="KAVEIESVLKAFVKVFGENKADLIPLNKEALLKGAQAAKK"
     gene            332..1354
                     /locus_tag="PKH42_07100"
     CDS             332..1354
                     /locus_tag="PKH42_07100"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOA20236.1"
                     /translation="MEKKDIKWSELGFGYIKTDKRYYSYWKDGKWDDGQLSEDNKVHI
                     SEGSTCLHYGQECFEGLKAYSTKDGGIQLFRPDENAKRMQKSCERLLMPIVPVEKFID
                     ACVQVVKANAAWVPPYGSGATLYLRPYVIGIGDNVGVKPAEEYLFGIFCTPVGPYFKG
                     GMNPVNFITTDQDRAAPNGTGKIKVGGNYAASLYSHFEAVKKGFADCIYLDPSTHTKI
                     DEVGAANFFGITKDNKFVTPRSSSILPSITRISLMQVAKDYLNMEALETDIYVDELDN
                     LKEAGACGTAAVITPIGGIMHKDKFHVFYSETEVGPVTKKLYDTLYGIQMGDIEAPEG
                     WIYKVM"
     gene            1470..2558
                     /gene="queG"
                     /locus_tag="PKH42_07105"
     CDS             1470..2558
                     /gene="queG"
                     /locus_tag="PKH42_07105"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOA20237.1"
                     /translation="MKQIIKEAAYKLGIETVGITGISDYSYLEELLTNRKINNQCCEF
                     EEQDIKKRINAKNLFPELKSIIAIGIPYGEGYKLPSCRNKGLLSVSCHGEDYHKTVNS
                     LLVKLAEEIMRHKSFKFLPCVDTSYLIDKEICRSAGIGSYGKNSLLINKNYGSFINLG
                     YLLTDIETETDIISEKDICGTCNLCVKSCPNKAIFEIGGINPKKCISYLTQTKNYIPL
                     EYRENMGRQIYGCDICQIVCPLNKEVLNKEVNTVYSDLIVDLEELLNISNKGFINKYG
                     HTAGSWRGRNIWKRNALISIGNLNIKSLYQNVKDELQNQSEIIRCYAAWSLLKLNRPL
                     AEEILYSNLKYENDYVKNEYLKLLEAKL"
     gene            2555..2836
                     /locus_tag="PKH42_07110"
     CDS             2555..2836
                     /locus_tag="PKH42_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOA20238.1"
                     /translation="MIVCSCEIEILIYESNSLKEKRQVIKSIIERIKSKFNASVAEVG
                     YNELWNRSLIGIAVVSNNKRVCDESISKIVKFIDYDERVEILSCNKEII"
     gene            2833..3471
                     /gene="nth"
                     /locus_tag="PKH42_07115"
     CDS             2833..3471
                     /gene="nth"
                     /locus_tag="PKH42_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOA20239.1"
                     /translation="MMTKTEKVYDLLKKAYPDAKCGLVFNTPLQLLISTILSAQATDK
                     SVNKVTEELYMQYPDLDSLLTLSHEEIAQKIKQIGLYRTKSKNIYNMLRVLKNKYKGK
                     VPKNMDDLMSLPGVGRKTASVVLAEAFNIPAFPVDTHVFRITRRIGIAKENTADKVSD
                     EMMKKLPKYKWHLMHHLLITHGRTTCTAKSPKCTICNVNEICTYYKKVVKEK"
     gene            3553..4023
                     /gene="trmL"
                     /locus_tag="PKH42_07120"
     CDS             3553..4023
                     /gene="trmL"
                     /locus_tag="PKH42_07120"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOA20240.1"
                     /translation="MSLNIVLFEPEIPQNTGNIARTCAATGSKLHLIKPLGFSVNDKY
                     LKRAGLDYWNLVDITYYENFEEFKKTTGNNKVFISTTKESRFYTEVAYSDECYIIFGK
                     ETAGLPGYIHEEYIKNRIKIPMVQKVNARSLNLANSVNIIVYEALRQLGFPQMI"
     gene            4032..5633
                     /locus_tag="PKH42_07125"
     CDS             4032..5633
                     /locus_tag="PKH42_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOA20241.1"
                     /translation="MDIAISLVGGLGLFLFGMIYMGDGLQKAAGNKMKDILAALTKNK
                     LMGVLVGALVTGVIQSSTATTVMVIGFVNAGLMNLNQAVGIIMGANVGTTVTALLVSL
                     NITKIATFMAGVGVIVYLIAKKRKVKNLAEVIIGFGILFIGMDLMKNAMEPLQSNPIF
                     VNLITKFDNPYLGIVVGFVMTAILQSSSATTGLLIAVAATGVISFEQAYPIVFGQNIG
                     TCLTALLASIGANKTAKRAALIHLLFNVLGTILFTLFLRIPVQRLVYMAAPVYVPRQI
                     AFGHVFFNIINVIILFPFSDFIVKASEFIIRKDGRETESVTKYIDERILATHSIALIQ
                     TAKEVLHLGNLVMDQFETAKKALFENNEELAYEVFDKEKKINRLHKEILEYLVKLDKV
                     SLTSAEKDKLFVLMNATSDIERVGDHIDNIGELLLYKFENKADFSEEATNEIANMFKI
                     TMEAYKLSLDSLASAESDVCRAVVKYEDDIDKMYKTLRKNHIDRLNNNICNPNAGIVF
                     LDMISNLERIGDHSLNIAEYILEVI"
     regulatory      5767..5874
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            6002..6361
                     /locus_tag="PKH42_07130"
     CDS             6002..6361
                     /locus_tag="PKH42_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HOA20242.1"
                     /translation="MIITNNDWVLNKYKDIYNVVYIDGTFKDVLIAVRDKIHIGHELL
                     THPLGSSVKPSETPYKSVIVSDNKNYTSFDSIYMIENAIITYDKFNKDKLKLISDKVK
                     EDLKLIDLTVLESALDI"
     gene            6392..7672
                     /locus_tag="PKH42_07135"
     CDS             6392..7672
                     /locus_tag="PKH42_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOA20243.1"
                     /translation="MKLELGYIFIKDIQFSDVSKVENGTLYVNKEEVKALILEDQNFK
                     TADVELAKPGESVRIMPVKDVIEPRVKVEGPGGIFPGMVSKVDTVGSGKTNVLKGAAV
                     VTTGKIVGFQEGIIDMSGTGAEYTPFSKLNNLVIICEPIDGLKQHEHEKALRFAGYKV
                     ALYLGALAKDLTPDEVEVFETPNLVEGIKMYPELPRVAYVFMLQSQGLLHDTYVYGVD
                     AKQTLTTMIYPTEVMDGAIVSGNCVSACDKNTTYHHLNNPIIYDLFKEHGKTLNFVGV
                     IITNENVYLADKERSSNWSAKLAGFLGVDGVIVSEEGFGNPDTDLIMNCKKIEAKGIK
                     TVLVTDEYAGRDGTSQSLADADVSANAVVSAGNANEVIELPPMEKIIGHVEVADVIAG
                     GFDGSLHKDGSITAELQVITGATNEMGFNKLSAR"
     gene            7689..8729
                     /locus_tag="PKH42_07140"
                     /pseudo
     CDS             7689..8729
                     /locus_tag="PKH42_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
BASE COUNT         3224 a         1227 c         1793 g         2519 t
ORIGIN      
        1 ctaaagcggt tgaaattgaa tcagtattga aagcttttgt aaaagttttc ggagagaata
       61 aggccgatct gatacctttg aacaaagaag ccttactgaa gggtgctcag gcagcaaaaa
      121 aataaagcgt ttagaaaata agtgttggaa attttaatca aagctgttat gatataaaaa
      181 tattatatgt attggggcaa caaggtatgg ggacacacct ctcctctgta gggtaaacct
      241 atgaagtcag agaaatgtcc ccaaatttaa agtctgtgag ggcagaggaa tgtcccgaaa
      301 taataaatat aaaaataaag gaggcttatt aatggaaaaa aaggacatca aatggtcaga
      361 attgggattt ggttatatta agacagacaa aagatactat tcttattgga aagacggaaa
      421 atgggatgac ggacagcttt cagaggataa taaggttcat ataagcgaag gctccacatg
      481 tctgcattac ggacaggaat gttttgaagg attgaaagct tattctacta aggatggagg
      541 tattcaactt ttcagacctg atgaaaatgc aaagcgtatg caaaaaagct gtgagagatt
      601 actgatgccc attgttcccg tggaaaaatt tatcgatgcc tgtgtgcagg tcgttaaagc
      661 taacgcagca tgggttcctc catacgggtc aggtgctaca ttgtatttga gaccgtatgt
      721 aataggtata ggagacaacg taggcgtaaa acctgcagaa gaatacttat ttggaatatt
      781 ctgcacacct gtcggcccat actttaaagg cggaatgaat cccgttaatt tcattactac
      841 cgaccaagac agagcagcac cgaacggaac aggaaaaatt aaagtaggcg gaaactatgc
      901 agcaagcttg tattcacatt tcgaagctgt taaaaagggt tttgccgatt gtatttattt
      961 agatccctca acccatacaa aaattgatga agttggagca gcaaacttct ttggcattac
     1021 caaggacaac aagtttgtaa cacctcgttc atcttcaata cttccgagta taacaagaat
     1081 ttctttaatg caggttgcta aagattactt aaatatggaa gcacttgaaa cagatatata
     1141 tgttgatgaa ttagataatt tgaaagaagc aggggcttgc ggtacggcag cggttattac
     1201 accgataggc ggaattatgc ataaagacaa attccacgta ttctacagtg aaacagaggt
     1261 aggacctgtt actaaaaaat tatatgacac attatatgga attcagatgg gtgatataga
     1321 agcgcctgaa ggatggatat acaaagtaat gtaatacttt tctccaaatg gacattaaat
     1381 ttacatattt aatgtccaat ttttttttct tcaatatttt atatatttat gatatgctat
     1441 ggtatggaaa caatagagat gaggatatta tgaaacaaat aattaaagaa gcagcatata
     1501 aattaggaat cgaaacagta ggtataaccg gcatttcaga ctattcctat ttagaagagc
     1561 ttttaacaaa cagaaaaatc aataatcagt gctgtgaatt tgaagagcag gatataaaaa
     1621 aaagaataaa tgccaaaaat ctctttcccg aattaaagag cataattgct attggtattc
     1681 cttacggaga aggttacaaa cttccttcat gcagaaataa aggtttatta agcgtatcat
     1741 gtcatggaga agattaccat aagacggtta attctttatt ggttaagctg gctgaagaaa
     1801 taatgcgtca taaaagtttt aaatttcttc cctgtgttga cacatcatat ctgatagata
     1861 aagaaatatg cagaagtgca ggtataggaa gttatgggaa aaacagcctg ctcataaata
     1921 aaaattacgg ctcatttata aatttaggct atcttttgac tgatattgaa acagaaacag
     1981 acattataag cgaaaaggat atatgcggta catgcaatct atgtgtaaaa agctgtccta
     2041 acaaggcaat atttgaaatc ggaggtatta atccaaaaaa atgcatttct tatttaactc
     2101 agacaaaaaa ctatatacct ttggaatacc gagaaaatat gggaagacaa atatatggct
     2161 gcgatatatg ccaaattgtc tgccctttaa ataaagaggt tctgaataaa gaggtaaata
     2221 cagtttattc agacctaatt gttgatttgg aagaattgct gaatatatca aacaaaggtt
     2281 ttataaataa atatggacac accgcaggca gctggagggg cagaaatata tggaagagaa
     2341 atgctcttat ttctataggg aatcttaata ttaaatcatt gtatcaaaat gttaaagatg
     2401 agcttcaaaa tcagtcggag ataataaggt gctacgcagc ttggtcttta ttaaaattaa
     2461 acagaccctt ggctgaagag atactatata gtaatttaaa atacgaaaat gattatgtaa
     2521 aaaatgaata cttaaaacta ttggaggcaa aattatgata gtttgctcat gtgaaattga
     2581 aatattgata tatgaatcta attccctaaa ggaaaaaaga caagtaatta aaagtataat
     2641 agaaagaatt aaatcaaagt tcaatgcttc cgtagcagaa gtcggatata atgaattatg
     2701 gaacaggtcc ttgataggaa ttgcagttgt atcaaacaat aaaagagtat gcgatgaatc
     2761 aatatcaaag atagtcaaat ttatagacta tgatgaaaga gtggaaatac taagctgcaa
     2821 taaggagata atataatgac caagacggaa aaagtgtacg atttgttaaa aaaagcttat
     2881 cctgatgcaa agtgcggact tgtttttaat acccctcttc agctgttaat ttcaacaatt
     2941 ttaagtgcac aggctacaga taaaagtgtt aataaggtaa cagaggagtt gtacatgcaa
     3001 tatcctgatt tggatagtct attaacttta tctcatgaag aaattgcaca aaaaattaaa
     3061 caaataggat tatatagaac aaaatccaag aatatttaca atatgctgcg agtgctgaaa
     3121 aacaaatata agggaaaagt gcctaaaaat atggatgatc tgatgtctct gccgggtgtc
     3181 ggaagaaaga ctgcttccgt agttttggcc gaagctttta atatacctgc ctttcctgta
     3241 gatactcatg tttttagaat aaccaggcga ataggaattg caaaggaaaa taccgcagac
     3301 aaagtatcgg atgaaatgat gaaaaagctc ccaaaatata agtggcacct catgcaccat
     3361 ctgttaataa cacacgggag aacaacatgc actgcgaagt ctcctaaatg tacaatctgc
     3421 aatgtaaatg aaatatgtac ttattataaa aaagtagtaa aggaaaagta aattttcttg
     3481 tatttactaa aattatgcct tattataatc agggaatgtc tatttaatta ataacgtagg
     3541 agaaaataat aaatgtcatt aaatatagtt ttattcgagc ctgaaatacc gcaaaatacg
     3601 ggtaatattg caagaacatg cgcagctaca ggctcaaaac ttcatttaat aaaaccatta
     3661 ggtttttcgg taaatgataa atacttgaaa cgtgcaggac ttgattattg gaatttggtc
     3721 gacattacat attatgaaaa ttttgaagaa tttaagaaaa caaccggaaa taataaagta
     3781 tttatttcaa caacaaaaga gagcaggttt tatacggaag tagcttattc tgatgagtgt
     3841 tatataattt ttggaaaaga aactgcaggt cttcccggtt atattcacga agaatatata
     3901 aaaaacagga ttaaaattcc aatggttcaa aaggtgaatg caaggtcatt aaatctggct
     3961 aattctgtta atataattgt ttatgaagct ttaagacaat tgggatttcc acagatgata
     4021 taggaggaac tatggatatt gctattagtt tagtaggagg attagggtta tttctttttg
     4081 gaatgatata tatgggggat gggctccaaa aagctgcagg aaataaaatg aaggatatat
     4141 tggcagcttt aacaaaaaat aaattgatgg gcgtcttggt tggggcactt gttacaggtg
     4201 taatccaaag cagtactgca acaactgtaa tggttatcgg ttttgtaaac gcaggtttaa
     4261 tgaatttaaa ccaggcggtg gggataataa tgggagcaaa tgtcggtact acagtaacag
     4321 ccctattagt atcccttaat ataactaaaa ttgctacttt tatggcaggt gttggagtaa
     4381 ttgtatacct tattgcaaaa aaacgaaaag ttaaaaattt ggctgaagta ataataggtt
     4441 ttggcatctt gttcataggc atggatttaa tgaaaaatgc aatggagcca ttacagtcaa
     4501 accctatatt tgtaaattta ataactaaat ttgataatcc ttatttaggt atcgttgtag
     4561 gttttgtaat gactgccata ctacaaagca gcagtgccac aacaggttta ttgatagcgg
     4621 tagcagcaac aggagtaata tcatttgaac aagcttatcc cattgttttc ggtcagaata
     4681 taggaacatg ccttacagcc ttattagcaa gtataggtgc caataaaact gcaaagagag
     4741 cagcattaat acatttgttg tttaatgtat taggaacaat tttgtttacg ttatttttga
     4801 gaatacctgt tcaaaggtta gtatacatgg cagctcctgt atacgtaccg aggcagatag
     4861 cttttgggca tgttttcttt aatattataa atgttattat attgtttcct ttttccgatt
     4921 ttattgttaa agcatcagag tttattatca gaaaagatgg tcgtgaaact gaaagtgtaa
     4981 caaaatacat tgatgaaaga atattggcaa ctcattcaat tgccttgatt caaacggcaa
     5041 aggaagtact gcatttggga aatttagtaa tggaccaatt tgaaactgca aaaaaagcgc
     5101 tatttgaaaa taatgaggaa ttagcatatg aggtttttga taaagagaag aaaattaaca
     5161 gattgcacaa ggaaatatta gaatatcttg ttaaacttga taaggtttca ttaacaagtg
     5221 ccgaaaagga taaattgttc gttttgatga atgcaaccag tgatattgaa agagtaggag
     5281 atcatataga taatattgga gaattgttgt tatataaatt tgagaacaaa gcagactttt
     5341 cggaagaagc tactaatgaa atagctaata tgtttaagat tactatggaa gcatataagt
     5401 tatccctgga ttcattggca agtgctgaat cggatgtttg cagggcggtt gttaaatatg
     5461 aagatgatat tgacaaaatg tataaaacac taaggaagaa tcacatagac agacttaata
     5521 acaatatttg caatccaaat gcaggaatag tatttttgga tatgataagc aatttggaaa
     5581 ggattggaga ccactcatta aatatagcag aatacattct tgaggtaata taaataaggg
     5641 cagatatcgc ttaatttagg ctgtaagaag tactaaatat ttgaaaatat ttgtaaaaaa
     5701 tagttgcttt atataagaaa attttatata ctaatactag tgataattga ataattgaat
     5761 aattgaatga aattcgcggg agagattgtt atctaattaa caacgccgaa ggacataagt
     5821 tacaaattag ccgtttgtaa tgactatctc aggcaaaagg accgtgaatt gacaaatctc
     5881 tggaaagcgc aagcaccgaa ggggcaattc caaaggaaga aactctcagg tataacaaca
     5941 gggcaggagg caggatatat ttgattttgc tttctgccct tatttttaac aggtgaaaaa
     6001 aatgattatt acgaacaatg attgggtatt aaataaatac aaagacatct ataatgttgt
     6061 ttatatcgac ggtacattta aagatgtttt gattgcagtt agagacaaaa tacatattgg
     6121 ccatgaactt ttgacacatc ccttaggcag cagcgttaaa ccaagcgaaa ccccttataa
     6181 atctgtaatt gtttcagaca acaaaaatta tacaagcttt gattcaattt atatgattga
     6241 aaatgcaata attacatatg ataagtttaa taaagataaa ttaaaattaa tcagcgataa
     6301 agtaaaagaa gatttaaaat tgattgattt aacagtgctg gaaagcgcgt tagatatata
     6361 actctttaaa aaactaatgg aggtgaagat tttgaaatta gaattaggct atatttttat
     6421 taaggatatt cagttttctg atgtatccaa ggtagaaaac ggtacgttgt acgttaacaa
     6481 ggaagaagta aaagcattaa ttctagaaga tcaaaatttc aaaacagcag atgtagaact
     6541 ggcaaagccg ggagaaagtg ttcgcataat gcctgttaaa gatgttattg agccaagagt
     6601 taaggttgaa ggccccggag gcatattccc gggaatggtt agcaaagtag atactgtagg
     6661 ctcaggcaaa acaaatgttt tgaagggtgc agctgttgta actacaggca aaatagtagg
     6721 tttccaagaa ggtatcattg atatgagcgg aacgggagcc gaatatacac ctttttccaa
     6781 gcttaacaac ttagttataa tttgcgagcc tattgacgga ttaaagcagc acgagcatga
     6841 aaaagcccta agatttgcag gatacaaggt agcattatat cttggagctc ttgcaaagga
     6901 tttaactcct gatgaagttg aagtatttga aacacctaat ttggttgaag ggataaaaat
     6961 gtatcccgaa ctaccaagag tagcttatgt attcatgctg caaagtcaag gtcttttgca
     7021 tgatacttat gtttatggag ttgatgcaaa acaaacatta acaaccatga tttatcctac
     7081 cgaagttatg gatggtgcca tagtaagcgg taactgtgtt tctgcatgcg ataagaatac
     7141 tacatatcat catttaaaca atcctatcat atatgatttg tttaaagaac atggcaagac
     7201 cttgaatttt gtcggagtaa tcataactaa cgaaaatgtt tatcttgccg ataaggaaag
     7261 atcatccaac tggagtgcaa aacttgcggg atttcttggt gttgacggtg ttattgtatc
     7321 agaagaagga tttggaaatc ctgatacgga cttgataatg aactgtaaga aaattgaagc
     7381 taaaggtatt aaaacagttc ttgttacaga tgaatatgca ggcagagacg gtacttctca
     7441 gtcattggca gatgcagatg tatcagctaa tgcagtggtt tccgcaggaa atgcaaatga
     7501 agtaattgag ctgcctccta tggaaaaaat aatcggtcat gttgaggttg ctgatgtaat
     7561 agccggagga tttgatggaa gcttgcataa agatggaagc ataacagcgg aattgcaggt
     7621 tattacaggt gctactaatg aaatgggctt caataaactt agtgcaagat aggaaggagg
     7681 agaaaataat gctaaaagta gttcattata ttaatcaatt ctttgccaat gtaggcggag
     7741 aagaaatggc tcacattcct gctgaagtaa gagaaggtgt agtaggaccg ggtcttgcat
     7801 ttcaacaagc atttaaggat gaagcagaaa ttgtagcaac aattatatgc ggagactcat
     7861 atttcaacga gaatatggat gaagccaaaa ataccataat tgaaatggtc aagaaataca
     7921 acccggattt atttattgca ggaccggcat ttaatgctgg cagatacggt gtagcttgcg
     7981 gcgctatttc aaagacagtt aaggatgaat tgaatatccc tgttataacg ggaatgtatg
     8041 tggaaaatcc cggagcagat atgtttaaaa acgacatcta tatagtatcc acaaagaatt
     8101 ctgctgcagg aatgagagat gctgttaaga aaatggctcc attaggactt aaacttgcaa
     8161 agggagaaaa aatcggcgca tcaatagaag aaggctacat accaagcggt aaaagagtta
     8221 atttctttga aaaagaaaga ggctcaacaa gagctgttaa aatgcttctt gcaaaattaa
     8281 acaacaaaga atttgaaaca gaatttccaa tgcctgattt tgacagagta cctccaaatc
     8341 cggctataaa agatattaca aaagcaaaaa ttgcattggt tacttccgga ggaattgttc
     8401 cgaaaggaaa tcctgaccat attgaatcat caagtgcttc taaatacggt aaatatgata
     8461 tcgaaggagt tatggattta accgaagaaa catatcagac agctcacggc ggatatgacc
     8521 ctgtttatgc aaatttagac cctgatagag tattgcctgt tgatattatg agagaatttg
     8581 aaaaggaagg caaaatagga agtcttcaca gatatttcta taccacggtt ggtaacggaa
     8641 ctgccgtaaa gagtgctaaa ggttttgccg aaaaatatgt aaaagaactt atagctgacg
     8701 gagttgatgc agttatactc acatctacct gaggtacctg tacacgttgc ggtgcaacga
     8761 tgg
//
LOCUS       DAOPUE010000073         8726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8066_length_8726_cov_9.293622, whole genome shotgun sequence.
ACCESSION   DAOPUE010000073 DAOPUE010000000
VERSION     DAOPUE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8066_length_8726_cov_9.293622"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1764
                     /gene="nhaC"
                     /locus_tag="PKH42_07145"
     CDS             343..1764
                     /gene="nhaC"
                     /locus_tag="PKH42_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005902038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOA20244.1"
                     /translation="MENDSKRPSLGVALLIVAFMFIVIIAQLIAVGSPDIHLTLVFSI
                     TFAVLLLLFGGTKWSVIEDGIMHGCKIAMIPMLILMFIGMLIPTLIAAGTIPALIYYG
                     LKIINPSVFLLTAALVCSVSSLSTGSSYTTGGTFGVAFMGIGMGLGIPPAMTAGAVIS
                     GAVIGDKISPISDSTNLAAGVTETNLFKHIKSMLYTTVPAFIISCILYTFLGMNYSAS
                     SIDTTAVDTILSGISSTFSVSFGSTLISMIPLAVVIFMAVKQYSALATMVAASLVAMI
                     IAMLTQGFSIIEMMSYMNYGFSIDVGVEAVNRLLNRGGIQSMMWTVSLGFLGLSFGGI
                     LEKSRAMETLLDSMKGLTKNAANLITTHVFSTIIVNMLSASQYVSIIIPGRMYLPAYR
                     KLGIRTDVASRTCEDAGTVTSPLVPWGLCGVFFTGVLGVNTLEYFPYAFLAYITPVIA
                     IIYAYTGKFVWYEEVSEKAADID"
     gene            1827..3029
                     /locus_tag="PKH42_07150"
     CDS             1827..3029
                     /locus_tag="PKH42_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOA20245.1"
                     /translation="MYKAMYKVDKAQITKLLIDLVNIESPYFEEDKIIEFIYRWFKQN
                     NIDSFIHEYHEAKVTGFKGKNIVVNLEGNKCGPVICLNGHLDTVKLCNGWTYNPKGEL
                     IGDRLYGVGALDMKSGCAATMIALKGFVENHKEFNGKIIATFVSVEEGPYGMGTNALI
                     EEGYIKDVDFSIITEPSSGFTGNPYPDVSFGARGGYGLEVEFFGKSSHAATPELGINA
                     AEDASRVITELKNVEYIEDPHLGKGSACVVAVESDGGACSVPDYAKIRLFWHIVMGES
                     EETITNEIRKAIERAQIKSSYQINFREAPSEGSRGFLPYTITGEEPITKIFLDAVEEV
                     CETKPTISYFQSIGDFNYLGSRLDAPAVIFGAAGGNFHGSDEYVELESVVKTAEVIYA
                     FLEKVLCP"
     gene            3207..4742
                     /locus_tag="PKH42_07155"
     CDS             3207..4742
                     /locus_tag="PKH42_07155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOA20246.1"
                     /translation="MRADILIKNGRCMTMKDREIMDWIAIKKSKIIAVGHGLEYESFV
                     GNETVVFDVNGSTVLPGFIDSHFHMVQTALNSVSLDLSGVKTHEEVGEKIKEASNSFM
                     GDYIRGIRISEGQFQEKVYPGRIFIDKYCNDVPVVLNSMEYQVSILNTCALLYFKIPF
                     TAEGIEMDRKKMPTGIFRKHANAILRTNILKNISDSTRKDATSEIMSTLLMNGITTVN
                     AMEGGFMYCDKDAEFVFEHGNDFPIDMVLFYQTMDIDRIKAMGLNRIGGCLYIDGTFG
                     ARTAALSFEYADCPGTMGSLRMSQQELNDFVIQCYKNNLQLALYTIGDRAIESALIAH
                     ENALYQTGITGLRHRLEHAELVNDNQIERAKGLNIIFSMQPSYETYWGGRGKMYEQRL
                     GENYKRTNPFKKIIDKGVVICGGSDSDVTEPRPLLGIHSAVNHPVAEHRVDIYEALKM
                     FTCNGAYGIFEENNKGTLEVGKLADVVILDQDILSVPSNDIDKIKVNVTIKSGEILYN
                     SLG"
     gene            4760..5926
                     /locus_tag="PKH42_07160"
     CDS             4760..5926
                     /locus_tag="PKH42_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOA20247.1"
                     /translation="MLNLSFFGKSKVEYNGKEIGDRLGNKAIALICLLVLNERRYLSR
                     EKIIGYLWPDSNIEAAKYNLRYNLWLIKKNIAEDKNHNLFLKVDNDCCSINNNYEFNC
                     DIIDIMKFKPSREDSVESLLKLKKLFRGDLLEGCYFNKCDEFNDLIIYERINFEQRKV
                     RILQRLVEVYENDKRYDDCIEILYEIMEIEPYDEKIALKLMDIYQKSGKRAVAINYFN
                     KFSYSLSCDLGINPSNELKNKYNEIKMAVSGDEFNDETNYNVINKDTNLKIVSYCIKN
                     VEYFWMADVIDKIIDSGVEDCIQQLSQKQLLDLSSIQSSISKFCNDNIDIINYRREIM
                     DVCIINSFIKLVEAVCRKRNVTIVILNYCDIDEISANVVEYLRKIKIKGLDIIE"
     gene            complement(5995..6921)
                     /locus_tag="PKH42_07165"
     CDS             complement(5995..6921)
                     /locus_tag="PKH42_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOA20248.1"
                     /translation="MKKKDILLSVLIVFLWGVNFTVIKLGLSGVPSMLLVSLRYLLST
                     FPAIFFIEKPKTEMKYIVLYGLTVGVGQFSCLFYAMQIGMPAGIASVVLQTQAFISPV
                     FAMIFLNEKLKSRQIIGFIVSAIGLIFIAMASIGDGMNNVPVLAFILMLAAPTFWAVS
                     NIIARIASDKAAAKGEKLNMISMVVWSGLIPPIPMFGLALMFDTPETLLGILTNLSGI
                     SIFAALYLAYGATLIGYGIWSILIASYPLNKVAPITLLVPIVALLSARIILTEQLSPL
                     QWIGFIVILTGLAISNANININKFKKDLHFGR"
     gene            7105..7326
                     /locus_tag="PKH42_07170"
     CDS             7105..7326
                     /locus_tag="PKH42_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOA20249.1"
                     /translation="MAEIKYEITEELAVLSESSRGWTKELNMVSWNDREPKFDIREWA
                     PNHERMGKGVTLNREEMKKIKDILNEIDL"
     gene            complement(7412..8302)
                     /locus_tag="PKH42_07175"
     CDS             complement(7412..8302)
                     /locus_tag="PKH42_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOA20250.1"
                     /translation="MQDTKKKSKLGFLVKLIIFIVLVISVTNTAYIVRENEYAYVTRF
                     SKFVKIQDTAGLHFKLPLLDKIEKIPQYRMMYDIPPSEVLTGDKKTLVVDNFTVWQID
                     DPYTFMRTVSRISEMENRIDAIVYNAVKNTLGTMDQTTIINSDNSSIDDVNSKITELV
                     NAQLKNYGVSTVAVEIKRFDLPKDNESAVYNRMISERTQMAESFRAEGNLEASKIVNE
                     TDKEVAILLSKASARAEELKGQGESEYMKIIAEAYSTEDRMNYYEFIRSLEALKVTMK
                     GEKTLILPADSFIVRVLNGY"
     gene            complement(8302..>8726)
                     /locus_tag="PKH42_07180"
     CDS             complement(8302..>8726)
                     /locus_tag="PKH42_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOA20251.1"
                     /translation="GIQLLDVKIQDSEPPTEEVIQAFKEVETAKQEKETRINEALAYR
                     NKTLPAAESRADKLIREAESYRESKINEAKGDVARFNAMYTEYAKNKDITRTRMYLET
                     IEEILPGVKVYIDSSEGGVQKLLPLESFNINSNNGGEN"
BASE COUNT         2826 a         1320 c         1687 g         2893 t
ORIGIN      
        1 ataccgcttt tcataggtat tatggaaaac cctacaattt aaccaaacat tgtgagtatg
       61 gtcctatgtt tatgagtaag ctttaatagt aaatgtttta ataaagctgc tgtgctaagt
      121 atggctcagc ggcttttata gtttttatat ccaagagccg tatttagctt ggtgttttat
      181 acaacctttc gttttctgca ttccgtgttt aaaaggtgtt taattagtgt tcttaaaatt
      241 attaatcttt taaaaggtat ttttttgtta caaccttttt tccactattt aaagaacata
      301 ttaaatgtat tatttattca aataaaaaag gaggttaatt ttatggaaaa cgattctaaa
      361 agaccatcac ttggtgtggc attattaatt gtagctttta tgtttatagt tatcattgcg
      421 cagttgatag ctgtaggtag tcctgatata catttgactt tggtattttc aataactttt
      481 gcagtactgc tgttgttgtt cggtggaaca aaatggtctg taattgaaga cggtattatg
      541 catggatgca aaatagcaat gattccaatg ctgatactaa tgtttatcgg tatgcttatt
      601 cctacgctta ttgcagcagg aactataccg gcacttattt attacggttt gaaaattatt
      661 aatccttcgg ttttcttgct tactgccgca ttagtatgca gcgtgtcatc tttaagtaca
      721 ggaagctctt atacaaccgg aggaaccttt ggtgttgctt ttatgggtat aggcatgggt
      781 cttggaatac cacctgcaat gacagccggt gctgtaattt caggtgctgt tattggagac
      841 aaaatatcgc caatttcaga ttcaacaaat ttagcagcag gtgttactga aactaatcta
      901 tttaagcata ttaaaagcat gctgtacaca actgttcccg cgtttataat ttcatgcata
      961 ctttatacat ttctcggtat gaattacagt gcttcttcaa tagatacaac tgcagttgat
     1021 actatactga gtggtatttc gtcaacattc agcgtttctt ttggctccac acttatcagc
     1081 atgattccat tggcggtagt tatatttatg gctgttaaac aatatagtgc tcttgctaca
     1141 atggtggcag cgtctttagt agctatgatt atagcaatgc tgactcaggg tttcagcatt
     1201 atagaaatga tgtcatatat gaattatgga tttagtattg atgtaggagt tgaagcagta
     1261 aatagacttc ttaacagagg cggtatacag tctatgatgt ggacagtatc cttaggtttc
     1321 ttaggtcttt ctttcggggg tattcttgaa aaatcccgtg caatggaaac attgctggac
     1381 agtatgaaag gacttacaaa aaatgcagca aatctgatta ctacacatgt attttctacg
     1441 attatcgtaa acatgttatc tgcaagtcag tatgtttcaa ttataatacc cggaagaatg
     1501 tatctgcctg catatagaaa attaggcata agaactgatg tagcatcaag aacctgtgag
     1561 gatgcgggaa ctgtaacatc acctttggta ccttggggat tgtgcggtgt attctttact
     1621 ggagtacttg gagtaaatac cttagagtat ttcccatatg cgttcctggc ttatataact
     1681 cctgttattg caattattta tgcatatacc gggaagtttg tttggtatga agaagtttca
     1741 gaaaaagcag cagatattga ttaggggaga aatctgatag tgcaaaatga tataattaaa
     1801 aatgttaaag gacagtaggt gagcgtatgt ataaagctat gtataaagtc gataaggctc
     1861 aaataacaaa attgctgata gacttagtca atattgaaag tccttatttc gaggaagaca
     1921 aaattataga gtttatctac cgctggttta agcaaaataa tatagattct ttcattcatg
     1981 agtatcatga agcaaaagtt accggtttta aaggtaaaaa tattgttgta aatttagaag
     2041 gcaataagtg tggtcctgta atatgtctta acgggcactt agacactgtt aagctttgta
     2101 acggttggac ttataatccg aagggagaac taataggcga tagactatat ggagttggag
     2161 cattggatat gaagtcaggc tgtgcagcaa ctatgatcgc attgaaggga tttgtcgaaa
     2221 accataaaga gtttaacggg aaaataattg caacatttgt ttctgttgaa gagggtccgt
     2281 atggcatggg aactaatgcc cttattgaag aaggctatat aaaggacgta gatttttcaa
     2341 ttatcactga gccatcttca ggctttaccg gaaatccata tccggatgtc agctttggag
     2401 ctcggggtgg atatggtctt gaggttgaat tttttggcaa atcatcccat gcagccactc
     2461 ccgaattggg aattaatgca gcagaagatg cttcaagagt aataaccgaa ttaaaaaatg
     2521 tagaatatat tgaagacccc catctcggga agggttctgc ctgtgtggtg gctgttgaaa
     2581 gcgacggtgg tgcctgcagt gttcctgatt atgcaaaaat acgattgttt tggcatattg
     2641 taatgggaga aagtgaagaa accattacaa atgaaatcag aaaagcaatt gagcgagcac
     2701 agattaaaag cagttatcaa attaacttca gagaagcacc ttctgaaggt tccaggggtt
     2761 ttttgcctta tactattacg ggagaagaac ctataacaaa aatctttttg gacgctgttg
     2821 aagaagtgtg cgaaacaaaa cctactatat cttattttca aagtattgga gattttaatt
     2881 atttaggctc cagattggat gcacctgctg taatttttgg agccgcaggc ggtaacttcc
     2941 atggaagtga tgaatatgtg gaattggaat cagtcgtaaa aactgcagag gttatttatg
     3001 cttttcttga gaaggtactg tgcccataat tttttaaggt ctaagaaaag ccatcaagct
     3061 tttcttagac ttttatcatt aattaaataa aagctttacc ggctttttta ttctattgaa
     3121 aaatcggttt tatactggta taatatttgt tatatttaat tattcaactg cataaaaatg
     3181 aaacgacatt ttttggagga attaagatgc gtgcggatat tttgattaaa aatggaagat
     3241 gcatgacaat gaaagataga gaaattatgg actggattgc cattaaaaaa agtaaaataa
     3301 ttgcggtagg acatggctta gaatatgaat ctttcgttgg aaatgaaaca gttgtttttg
     3361 atgtgaacgg tagtacggta cttccgggat ttattgatag tcattttcat atggttcaga
     3421 cagctcttaa ttctgtaagt ttagatttga gtggagtaaa aactcatgaa gaagttggcg
     3481 aaaaaattaa agaagcctcc aatagcttta tgggagatta tattcgcgga attcgaattt
     3541 cagaaggaca attccaagaa aaagtatatc cgggaagaat atttattgat aagtactgta
     3601 atgatgtacc ggtcgttctt aattccatgg agtatcaggt aagcattctt aatacttgtg
     3661 ccttgttata ttttaaaata ccttttacag ctgaaggaat tgaaatggac agaaaaaaga
     3721 tgcctacagg aatcttcaga aaacatgcta atgctattct tagaacaaat atattaaaaa
     3781 acatatcgga tagcacaagg aaagatgcaa cttccgaaat tatgtctact cttttaatga
     3841 atggtattac taccgtaaat gccatggaag gcggatttat gtactgtgac aaggatgcag
     3901 agtttgtttt tgagcatgga aatgatttcc cgatagatat ggtgcttttt tatcagacaa
     3961 tggatattga tagaattaaa gcaatggggc ttaacaggat tggaggatgt ctctatatcg
     4021 atggaacctt tggcgcaaga acagcagctc tttcctttga atatgcagat tgtccgggaa
     4081 ccatgggcag tctccgtatg tcacagcagg agctaaatga ttttgtaata caatgctata
     4141 aaaacaatct gcagttagca ttgtatacaa tcggtgacag agcaatagaa tcagcactta
     4201 ttgcacatga gaatgcactg tatcaaaccg gtataacagg tcttcgtcac agattggaac
     4261 atgctgaatt agttaatgat aatcaaattg aaagggcgaa aggtttaaat attatttttt
     4321 ctatgcaacc atcatatgaa acatattggg gcggacgagg aaaaatgtat gagcaaaggc
     4381 taggagaaaa ttacaaacga actaatccgt tcaaaaagat aatcgataaa ggtgttgtta
     4441 tatgcggtgg ctcagatagt gatgttacag agccaagacc tcttttgggg attcattctg
     4501 ctgtaaatca cccggtagca gaacacagag ttgatatata tgaagcatta aaaatgttta
     4561 cttgcaatgg cgcatatggt atttttgaag aaaacaataa aggtacttta gaagtaggaa
     4621 aattggcaga tgttgtaatt cttgatcaag acatcctttc agtaccttca aatgatatag
     4681 ataagataaa ggtaaacgta actattaaat ctggtgaaat attgtataac agccttgggt
     4741 aaataaaaag gaaaatgata tgttgaatct aagttttttt ggaaaatcta aagttgaata
     4801 caatggtaaa gaaataggag accgtttagg aaataaggcc atagctttga tttgtctttt
     4861 agtgttaaat gagagaagat atttaagcag ggagaaaatt atcggatact tgtggccgga
     4921 tagcaacatc gaggcagcaa agtataactt gcgttataat ttatggctta taaagaaaaa
     4981 tatagctgag gataaaaatc acaacctatt tttgaaggtg gacaatgact gttgcagtat
     5041 aaacaataat tatgaattta attgtgatat tattgatatt atgaaattta aaccaagcag
     5101 agaggattct gtagaaagct tactaaagct taagaaactt tttcgcggag acttattgga
     5161 aggttgctat tttaacaagt gtgatgaatt taatgacctc attatatacg aaagaataaa
     5221 ttttgagcag cgcaaggtaa ggatacttca gcgtttagtt gaagtttatg aaaatgataa
     5281 aagatatgat gactgtattg aaatattata tgagataatg gaaattgagc cttatgatga
     5341 aaaaatagca ttaaaactta tggatattta ccagaaaagc gggaaacgtg cagttgcaat
     5401 aaattatttc aataaattca gctatagtct atcctgtgac cttggtataa atccctctaa
     5461 tgaacttaaa aataaatata atgaaatcaa aatggctgta tcaggtgacg aatttaatga
     5521 tgaaactaat tataatgtga ttaataaaga taccaatcta aaaattgttt cctattgtat
     5581 caagaatgta gaatattttt ggatggctga tgtaattgat aaaattattg attcaggtgt
     5641 agaagattgt attcaacaat tgagtcaaaa acaattgtta gatttaagct ctatacaaag
     5701 cagtatatca aaattttgca atgacaatat tgatataatc aattatagaa gagaaataat
     5761 ggatgtgtgt attataaatt cttttataaa gctggtggaa gccgtctgtc gtaaaagaaa
     5821 tgtgactatc gtgattttaa actattgtga catagatgaa atttcggcaa atgttgttga
     5881 atatcttaga aaaattaaaa ttaagggatt agatataatt gaataattat tataagctgc
     5941 agttcagttg aatgcagcta taattttatt atatacgttt tttgttgaac gagtttacct
     6001 tccgaaatgc aaatcttttt taaacttatt tatattaata tttgcattag aaattgctag
     6061 gcctgttaaa ataactatga atcctatcca ctgcaatggt gacagctgct cagtaagaat
     6121 aattcttgcc gacaataatg caactatggg aaccaatagg gtaatcgggg cgaccttgtt
     6181 cagggggtaa gatgctatca atatgctcca aataccatat ccgattaatg ttgcaccata
     6241 agctaaatat agtgctgcaa atattgaaat gccgcttaag tttgtcaata taccaagaag
     6301 ggtttcaggt gtgtcaaaca tgagtgccaa accaaacatt ggaattggcg gaatcagtcc
     6361 tgaccataca accatactaa tcatattcag tttttcaccc tttgcagctg ctttgtcaga
     6421 agcaattctt gcaattatat ttgaaacagc ccaaaatgta ggtgccgcta acatcagaat
     6481 aaaagctaag acaggtacgt tattcatacc gtctccgata gaagccattg ctataaatat
     6541 caaacctatg gctgatacta taaatccaat aatttgcctt gattttaact tttcgtttaa
     6601 aaatatcatg gcaaaaacag gcgatataaa agcttgagtt tgaagtacaa cggaagctat
     6661 gcctgcgggc attccgattt gcatagcata aaacaagcat gaaaattgcc ctactcctac
     6721 cgttagcccg tataacacaa tatatttcat ttctgttttt ggcttttcta taaaaaatat
     6781 tgccggaaat gttgaaagca gatacctcaa tgaaactaaa agcattgacg gaactccgct
     6841 taagcctaat ttaataacgg taaaattcac accccataaa aatactatta atactgacag
     6901 caatatatcc tttttcttca tttagttact cctaatgttg atatttcgat tataacatag
     6961 tttaaaatat tttgatatat aattaaatta aaattagttg gaaagttttt gatacaatgt
     7021 agtaattatt actgatttgc ggtataatat catgaatata ttattgatat tattatacgt
     7081 tttagaataa agatggaggg taaaatggct gaaattaagt atgaaataac cgaagaatta
     7141 gctgtgcttt ctgaatcatc gagaggttgg acaaaggaat taaacatggt cagctggaac
     7201 gatagggaac ctaagtttga tataagagaa tgggcaccga atcatgaaag gatgggaaaa
     7261 ggagtcacac taaacagaga agaaatgaaa aaaatcaagg atatactaaa tgaaattgac
     7321 ctttagtcaa aatatatccg gcttttcttg tttaaaggaa aagctcattc tataacagaa
     7381 tgagctttgt aattgtatgc tttagagcgc tttaatatcc gttcagtact cttacaataa
     7441 agctgtctgc aggcaatatt aaagtttttt cgcccttcat tgttacctta agagcttcca
     7501 ggcttctgat gaattcgtaa taattcattc tgtcttccgt tgagtaagct tcggctataa
     7561 ttttcatata ttcgctttcg ccttggcctt taagctcctc tgctcttgca gaagctttag
     7621 acagcagtat tgccacttcc ttgtctgttt cattgactat cttgctggct tccaaattac
     7681 cttctgctct gaagctttcc gccatttgtg ttctttcgga aatcattctg ttatatactg
     7741 cggattcatt gtctttcggc aggtcaaatc ttttaatttc aacggctacc gtagatacac
     7801 cgtagttttt cagctgagca ttaactaatt ccgttatttt actattaacg tcatcaattg
     7861 agctgttgtc tgaatttata attgtagttt ggtccattgt tcccaatgtg tttttaactg
     7921 cattgtatac tattgcatca atacgatttt ccatttcaga tattcttgat acggttctca
     7981 taaatgtgta ggggtcatct atctgccata ccgtaaaatt atctaccacc aatgtttttt
     8041 tgtcaccggt cagtacttct gaaggaggta tgtcatacat cattctatac tgaggtattt
     8101 tctctatttt atccaagagg ggaagcttga aatgcaatcc tgcagtatct tgaattttta
     8161 caaattttga aaatctggtt acataagcat attcgttttc tctgacgata taggctgtgt
     8221 tggtaactga tattaccaat acaataaata ttattaactt tacaagaaat cctaatttgc
     8281 ttttcttttt agtatcctgc attaattttc acccccattg tttgaattta tattaaagct
     8341 ttccaaaggc agtaattttt gtactccgcc ttctgatgaa tcaatatata cttttacgcc
     8401 gggaagaatt tcttctatag tctccaaata cattctggtc ctggtaatat ctttgttttt
     8461 ggcatattct gtatacattg cattgaatcg tgccacatca ccttttgctt cattaatttt
     8521 ggattccctg taagattcag cttctctgat taatttatct gctctgcttt cagccgcagg
     8581 cagagtttta tttctgtaag ccaaagcttc gtttatcctt gtttcctttt cctgttttgc
     8641 tgtttctact tctttaaaag cttgtattac ttcttctgtg ggaggctcac tgtcttgaat
     8701 tttaacatct aagagctgaa ttccca
//
LOCUS       DAOPUE010000074         8629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8179_length_8629_cov_8.041871, whole genome shotgun sequence.
ACCESSION   DAOPUE010000074 DAOPUE010000000
VERSION     DAOPUE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_8629_cov_8.041871"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..1012
                     /locus_tag="PKH42_07185"
     CDS             128..1012
                     /locus_tag="PKH42_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOA20252.1"
                     /translation="MFLLTYSYNSVQSAGFLNEEKSGIIPASEVLQGASNIDMIDIIK
                     IFNNFNLKELKNTINTYKDVISLNEVKVLSPIPFPKRNIICLGKNYLDHISEVKSLKN
                     VNSDIPQKPIYFSKIAYPAIGHEDVVNSHSNLVKQLDYEVELAIIIGKKCKNVSMEEA
                     EDIIFGYTIANDISARDIQTTHGQWHKGKSFDTFCPIGPYIVHKSSISFPPKLKIQSY
                     VNNELRQNGNTDNLIFDIPYIISDLSSGITLYPGDIILTGTPAGVGAGFNPPRYLKPG
                     DRVDCVIEGIGTLSNTIA"
     gene            complement(1238..1621)
                     /locus_tag="PKH42_07190"
     CDS             complement(1238..1621)
                     /locus_tag="PKH42_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HOA20253.1"
                     /translation="MKMVVISLIILLLMIGIWAWFYYGSVDPVTTYYWDSLIKLSDIV
                     RKEDWERAKRDISIYTDKWYEVKKTWVFFINQEDLDNIDSSIRQLNIYIENEEKILAQ
                     AELEHLIVLFYVIDENECLTIENIF"
     gene            complement(1624..2259)
                     /locus_tag="PKH42_07195"
     CDS             complement(1624..2259)
                     /locus_tag="PKH42_07195"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOA20254.1"
                     /translation="MIVAIRALILYAVLLIAMRIMGKGDLGELQPFDLVVSLVLAELA
                     VMPMEDLDAPLFHGLMATAVVMFLQCLISYITLKSNSARKVICGTPSVIYDHGKFNIK
                     DMNKLRINMNDVLGQMRLKGYYDFKDIDYLIMETNGDVSIIAPESKTAKRIKRIPIAV
                     ILDGKIMYNNLEKFDITKEDLDKSLKKENLRCKDVLYGYIDENDKFVFYKR"
     gene            complement(2444..3541)
                     /locus_tag="PKH42_07200"
     CDS             complement(2444..3541)
                     /locus_tag="PKH42_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HOA20255.1"
                     /translation="MIDKIVKAVIAVIGASLGAVSGVLLNNSGLLELPSLQSTLVIIG
                     ISLVFGIIFFLLSSKIKKTGLKIIEALETEILKFSTMDIVLGSLGLIVGFIIAFLISQ
                     PLSDIKYVGTIISVISFVFFGYLGVKIATRKKDDVLWPQILNLKKSSTVKKQEKNKKE
                     IISPKILDTSVIIDGRISDICKTGFIEGTLVIPEFVLKELRHIADSSDSLKRNRGRRG
                     LDILNILQKDDTVNVVIESKDYDDGIEVDVKLLKLAKELGGKVLTNDFNLNKVAEFQG
                     VEVLNINELANAVKPVVLPGEEMTVMIVKDGKESGQGLAYLDDGTMIVVEGGKKCIGD
                     SVVVTVTSVLQTAAGRMIFAKLKNVINNKAV"
     gene            complement(3744..4235)
                     /locus_tag="PKH42_07205"
     CDS             complement(3744..4235)
                     /locus_tag="PKH42_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOA20256.1"
                     /translation="MFNLGDKVVYPMHGAGVIETMESKEILGEIKNYYVLKMPIGEMK
                     LMIPVDNVNNIGLRNIIDKYAVEEVYKVLKQKAEINDSNWNKRYRDNMTKMKTGDIFE
                     VAQVVRDLTYRDREKGLSTGEKKMLVSAKQMLISEITLATGMDSEEIQDYMDEIINFD
                     ALE"
     gene            complement(4424..5353)
                     /locus_tag="PKH42_07210"
     CDS             complement(4424..5353)
                     /locus_tag="PKH42_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOA20257.1"
                     /translation="MAKATREAYGEALKKLAASNPDIVVLDADLSGSTKTSEFKKVSP
                     ERFFNVGIAEQNLIGTAAGLSLAGKIPFASSFAMFAAGRAFEIIRNTVAYPALNVKIA
                     ATHAGLTVGEDGGSHQAIEDISLMRTIPNMVVINPADSREAEQAVLRAAEYVGPVYIR
                     LGRMAVEDVYTDSYVFEWGKGVLLRDGKDVSIIATGLMVQEALKAHEILKEDGINAKV
                     INIHTIKPIDRDLIIRAAKETKAIVTAEEHSVIGGLGSAVLEVLSDEYPVPLKRVGIL
                     DTFGESGKPKDLLQKYRLTADEIVRQVKEVIKF"
     gene            complement(5346..6188)
                     /locus_tag="PKH42_07215"
     CDS             complement(5346..6188)
                     /locus_tag="PKH42_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOA20258.1"
                     /translation="MKYDELKVIAKSMRKNILTMIYKAKSGHPGGSLSAVEIMVYLYY
                     KEMKIDSPIDENRDRFVLSKGHGAPVLYAVLMEKGFISKDLIPTLRQIDSKLQGHPDM
                     KKLPGVEASTGSLGQGLSIANGMALAFKLDKKPNRVFALLGDGEINEGMIWEAAMLAS
                     HYNLDNLTAVLDHNGLQIDGKNEDVMTIEPIDKKFEAFGWHVIKTEGHSFESLEKAFE
                     ERKTIKGKPAIIIANTIKGKGCSFMENQAGWHGKAPNEGEFCKAMEEMGDIGDKTEGG
                     GYNG"
     gene            complement(6203..6847)
                     /gene="fsa"
                     /locus_tag="PKH42_07220"
     CDS             complement(6203..6847)
                     /gene="fsa"
                     /locus_tag="PKH42_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOA20259.1"
                     /translation="MKLFIDTANVEEIRKANDMGVICGVTTNPSLIAKEGRIFKDVVK
                     EITDIVDGPISAEVISLDSEGMIKEAVELSKIHKNIVIKIPMTAEGLKAVKVLSKEKI
                     KTNVTLIFSAGQALLAAKAGASYVSPFVGRLDDIGNDGMNIIKETVEIFKNYNIDTEI
                     IAASIRHPMHVIEAAKLGCHIATIPYKVIMQLTKHPLTDKGIDQFLKDWDTVPK"
     gene            complement(7051..7818)
                     /locus_tag="PKH42_07225"
     CDS             complement(7051..7818)
                     /locus_tag="PKH42_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOA20260.1"
                     /translation="MDKLSIMGVRINNISMNEVLKLAEQKIKNDEKYIIYTPNTEIIM
                     MCQKDEEFLNIMNKSDINVPDGVGLIYAGKIKKFPLKEKVAGYDLSINLLKMADEKGL
                     KLYAVGGRPGVAEAAMQNVQKKYPGIKIVGAHHGYFKGTHLGKFGHEEEIAVIEDINR
                     HKPHILFVGFGAKKQEQWIEYNKDLINANIIIGNGGTLDGLAGIVKRAPDIFINLGLE
                     WLYRLIKEPKRITRQIVLPVFMFKVIFGNKDIVKKIE"
     gene            complement(7811..>8629)
                     /locus_tag="PKH42_07230"
     CDS             complement(7811..>8629)
                     /locus_tag="PKH42_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOA20261.1"
                     /translation="LQDVTSKRSIYYYLFLIRLAKLMNNKVVMLSQGIGPIINEKSIK
                     AVKNTLSLVDYITVRDKHSKEFLESIGVDERKIFLSTDPVINIRKDDSLEYEKQSVRK
                     VCFSLRNWKDSDVSVKISKVAQRLIDDGIECHFIPFYYKEDLQLIAQVEELLGSKAVY
                     YKERLSTGDAFDIIKTMDVMVGVRLHSLIFAAAANVPIVAVSYDQKVDNFVNSVNMKI
                     ACTIDNIDENKIYDEIIDKINNEREEKIMLLKSVEKLRELTNINYKILKEITNG"
BASE COUNT         2506 a         1862 c         1252 g         3009 t
ORIGIN      
        1 acaaggggac gggggtagtg tcatcctttg tccaccgaga accgtccccg ctggacatcc
       61 acttttatct tgaaaaagta ttcttattat attatagtct atatagaata aaatattgga
      121 ggcctttatg tttttattaa cttattcgta taatagcgta cagtcggctg gttttctgaa
      181 tgaagaaaaa agcggcatta ttcctgcatc agaagtttta caaggtgcat cgaatataga
      241 tatgattgat attattaaaa tatttaacaa ttttaattta aaagaattaa aaaatacaat
      301 caatacatac aaagacgtaa taagcttaaa cgaagttaaa gttttatctc ccattccctt
      361 ccctaaaaga aatattattt gtttgggtaa aaactattta gaccatatca gcgaagtaaa
      421 aagcttaaaa aacgttaatt cggatatacc gcaaaaaccg atttatttca gtaaaattgc
      481 atatcctgcc atcggtcatg aagatgttgt taattctcat tccaatcttg ttaaacagct
      541 agactatgaa gttgaattag ccataataat aggcaaaaaa tgcaagaatg tttccatgga
      601 ggaagcagaa gacattatat tcggatatac catcgctaac gatatttccg caagagatat
      661 tcaaacaaca cacggtcaat ggcacaaggg caaatccttt gatacttttt gccctatagg
      721 accatacatt gtgcataaat caagtatatc attccctccc aaattaaaaa ttcaaagcta
      781 tgtaaacaat gagctcagac aaaacggcaa taccgataat ttgatatttg atataccata
      841 catcataagc gacctgtctt caggaataac actgtatccc ggagatataa ttttgacggg
      901 aactccggca ggcgtgggag caggattcaa tcctccgagg tatttaaaac caggagacag
      961 agttgactgt gttatcgaag gtataggaac cctttccaac accatagcct aaattacaat
     1021 gaaaagagga cggtaggcgt caaaaattgt catcctggag gacggtaggc gtcaaaaatt
     1081 gtcatcctga gcgtagcgaa ggatctcagg atctcaggat ctcagaatct caggttttat
     1141 aagatgagat tcttcgagct ggagctctca gaatgacaca tagcttattc acagtaatga
     1201 gaaaaagcgt atacacaacc agatttggat gctgtcatca aaagatgttt tctatagtta
     1261 gacattcatt ttcgtcaatt acataaaata atactattaa gtgctcaagc tctgcttgag
     1321 ccaatatttt ttcttcattt tcaatataaa tattaagctg ccgtatggag gagtcaatat
     1381 tgtcgaggtc ttcttggttt ataaagaaaa cccaagtttt ttttacttca taccatttat
     1441 cggtgtatat cgaaatatct ctcttagctc tttcccagtc ttcctttctt actatatcag
     1501 ataattttat taaactatcc cagtaatatg ttgtaacagg gtctaccgag ccatagtaaa
     1561 accatgccca tatacctatc atcaatagca atataatcaa cgaaataact accattttca
     1621 taattacctc ttataaaaaa caaatttgtc attttcatca atgtagccgt atagcacatc
     1681 tttgcagcgt aaattttctt tttttaaact cttgtcaagg tcttcctttg taatgtcaaa
     1741 tttctccaaa ttattataca taattttccc gtctaagatt acagcgatgg gaattctctt
     1801 aatccttttg gctgtcttgc tttccggtgc gatgatgcta acgtctccgt tagtttccat
     1861 aatcaaataa tctatatctt tgaagtcata ataccctttt aggcgcattt gacctaatac
     1921 atcattcatg ttaatcctta gtttattcat atctttaata ttaaactttc catggtcata
     1981 aattaccgaa ggagttccgc atataacttt tcttgctgaa ttgcttttga gtgtaatata
     2041 tgatattaaa cactgcaaga acataacgac ggcagtagcc attaaaccat gaaataaggg
     2101 agcatccaaa tcttccatag gcattacggc aagctccgcc aatactaatg atacaaccaa
     2161 gtcaaaaggc tgcagctctc ctagatcacc ctttcccatt atgcgcattg caatcagcaa
     2221 gactgcatac aatattaagg ctcttattgc aacaatcata atgacacctc ccaaaacgca
     2281 atcataaact atttttaccg aaacccttta ataatatacg tttaatatag ttaacgggaa
     2341 gttcgacaat tgggtggatt aggcatacaa aaaggacaca cctttcggtg tgtcctatga
     2401 gactcttcgt ggagattgtg acaatacccc cgtccccttg tcattaaact gctttgttgt
     2461 taattacatt tttcaatttt gcaaaaatca ttcttcctgc tgcagtctgc aaaacactgg
     2521 taacggtaac cactaccgaa tcgccgatgc atttcttgcc gccttctact acaatcatgg
     2581 taccgtcatc caagtaggcc aatccttgcc ctgattcctt tccgtctttg acaatcatta
     2641 cagtcatttc ttctccgggc agtacgacag gctttactgc atttgccagc tcgttgatgt
     2701 tcaacacttc aaccccttga aattcagcga ccttatttaa attaaagtca tttgtcagca
     2761 ccttgccgcc taattctttt gccaacttca aaagctttac atctacttca ataccatcat
     2821 cgtagtcttt tgattcaata acaacattta cggtatcgtc tttttgaaga atattcaaaa
     2881 tatccaaacc tcttcttccc ctgttgcgct taaggctgtc agaggaatct gcaatatgtc
     2941 tgagttcctt caatacaaat tccggaatga ctaaggttcc ttctatgaat cctgttttgc
     3001 aaatatcaga tattcttccg tcaataatta cactggtatc aagaattttt ggagatatta
     3061 tttctttttt attcttctct tgttttttaa ccgttgagct cttttttaaa tttaatattt
     3121 gaggccataa aacatcgtct ttttttcgtg tagcaatctt tacacccaga tatccgaaaa
     3181 atacaaatga aataactgaa ataatagtac ctacatactt aatgtcggat aggggctggc
     3241 ttataagaaa tgctattatg aatcctacta tcagccctaa tgatcccagc actatgtcca
     3301 tggtggaaaa tttcaatatt tcggtctcaa gagcttctat tattttcaaa ccggtctttt
     3361 taattttaga agaaagaaga aaaaatataa ttccaaaaac taaactaata cctataatta
     3421 ctaatgtact ttgcaaactt ggtaattcaa gaagaccgct gttatttaac aatacacccg
     3481 atactgctcc cagagatgca cctataacag ctataaccgc ttttacaatc ttatcaatca
     3541 tattatcacc tccttacaag tataaccatt aaaagttata ttatttagta ttatttataa
     3601 tgtaacatat tttttgatat ttggctataa ctattatcga aataatacaa taagaattca
     3661 ttaatattct attaaatttg acatccggct aaatgtatgc accaaaaatt ctgatgcaga
     3721 aaaaatgcat tagagcaatt ttactattct aatgcatcaa agtttataat ttcgtccata
     3781 taatcctgta tctcctcact gtccatgccg gttgcaagcg taatctcgct tatgagcatt
     3841 tgttttgcac ttactaacat tttcttttct ccggttgaaa ggcctttttc cctgtccctg
     3901 taggttaaat ctcttacaac ctgagcaact tcgaaaatat ctcccgtctt catttttgtc
     3961 atgttatccc tatatctctt attccagtta gagtcattaa tttctgcttt ttgttttaaa
     4021 accttatata cttcctctac tgcatattta tcgatgatgt ttcttaaacc tatattattc
     4081 acattatcca caggtatcat caatttcatt tcccctatgg gcattttcag tacataatag
     4141 tttttaattt cacctaagat ttccttggat tccatagttt caattacacc ggctccgtgc
     4201 atggggtata ctactttgtc gcctaaatta aacatgctgc acctcctatc aatacattat
     4261 acctcattag gcaacaaatg tcaaatcgaa tattatatcg tgataccgca tgcttgtcaa
     4321 caataaaaat aatttttact ttaaatttta ttaaagaata ttttacgttt ttttcataaa
     4381 tataagaatg cccctataac ggggcattcg aaattttaag cgcttaaaat ttaattactt
     4441 cttttacctg tcttacaatt tcatcagcag tcaatctata tttttgcaaa agatcttttg
     4501 gctttcctga ttctccgaat gtgtccaaaa ttcccaccct ttttaaagga acaggatatt
     4561 catcggacag aacttctaaa acagcgcttc ccaaacctcc gattacactg tgttcttcgg
     4621 cggtaactat tgccttggtt tctttggctg ccctgattat taaatccctg tctatgggtt
     4681 ttattgtatg gatattaata accttggcat ttattccgtc ttcttttaaa atttcgtggg
     4741 ctttcaatgc ttcctgcacc ataagaccgg tggcaattat ggaaacatcc ttgccgtctc
     4801 ttaataagac tccttttccc cattcaaata catatgaatc tgtatatacg tcttcaactg
     4861 ccattctgcc aagtctgata taaacaggtc ccacatattc ggcagctctt aaaacagcct
     4921 gttctgcctc cctgctgtcg gcagggttta taacaaccat attgggaata gttctcatta
     4981 aacttatatc ttcaatcgcc tggtgcgagc ctccgtcttc tcccacggta agtcctgcat
     5041 gggtagctgc aatcttcacg tttaatgcag gatatgcaac agtatttcta ataatttcga
     5101 atgcccttcc tgcagcaaac attgcaaagg agcttgcaaa cggaatttta cctgccaatg
     5161 acagccctgc tgccgtaccg atcaaattct gctctgcaat tcccacattg aaaaatcttt
     5221 cgggacttac ctttttaaat tccgatgtct tagtggagcc ggataaatcc gcatccaata
     5281 cgactatatc aggatttgat gctgctaatt tttttaaagc ttcaccgtat gcttctctgg
     5341 ttgccttagc cattatatcc gcctccttcc gttttgtctc cgatgtctcc catttcctcc
     5401 attgctttgc aaaactcgcc ttcattcggt gcctttccat gccagcctgc ctggttttcc
     5461 atgaaagagc aacccttccc cttgatagta tttgcaatta ttattgccgg tttccctttt
     5521 attgtttttc tttcttcaaa agctttttcc aaagattcga aactatgccc ttctgttttt
     5581 atcacatgcc atccgaatgc ttcaaatttt ttgtcaatcg gctcaattgt cataacatct
     5641 tcatttttgc catctatctg caaaccgtta tggtccaata cggcggttaa attatcaaga
     5701 ttgtagtggc ttgcaagcat tgctgcttcc caaatcatac cctcgtttat ctcaccgtct
     5761 ccaagaagtg caaataccct gtttggcttt ttgtctagtt taaatgccag agccatgccg
     5821 tttgcaatag aaagtccctg ccctaaggag ccggttgaag cttctacccc gggaagtttc
     5881 ttcatatcgg gatgaccttg aagcttgctg tcaatctgtc ttagagtggg aattaaatcc
     5941 ttgcttataa agcctttttc catcaatacg gcatacaaaa cgggagctcc atgtcccttt
     6001 gataatacaa atctgtctct gttttcatct attggagaat ctattttcat ttccttgtag
     6061 tacaaataca ccattatttc caccgcggaa agagagcctc ccggatggcc tgacttagcc
     6121 ttataaatca tagtcaatat attttttctc atacttttgg ctatgacttt taactcatcg
     6181 tatttcattt tttcctccaa gtttatttag gtaccgtatc ccagtctttc aaaaattggt
     6241 ctattccctt atcagtcaaa ggatgttttg tcagttgcat tattacctta taaggaatgg
     6301 ttgcaatatg gcatcctaac tttgcagcct caataacatg catcggatgc cttatgcttg
     6361 cagctataat ttcggtatca atattataat ttttaaaaat ttcaactgtt tctttaataa
     6421 tattcattcc gtcattacct atatcatcaa gccttcccac aaaaggactg acatagcttg
     6481 cgcctgcttt tgcggccaga agtgcctgac ctgcagaaaa tatcaaggtt acattggttt
     6541 ttattttttc cttgctcaaa acctttactg cctttaaccc ttcagcagtc ataggaattt
     6601 taataactat atttttatgt attttagaaa gttctacagc ttcttttatc attccttccg
     6661 agtccaagct gataacttca gcacttatcg ggccgtcaac tatatcggtt atttctttta
     6721 ccacatcctt aaatattctt ccttccttcg ctattaatga cgggtttgta gtaactccgc
     6781 aaataacccc catatcatta gcttttctta tttcttcaac attagccgta tcgataaata
     6841 atttcatttt ttcctcctat tttattattt acatcatttt tatcaataac ccacagagtg
     6901 tcattgttat gaataacatt tttgtcattc tgagggcttt agctcgaaga atctcatgag
     6961 atccttcgtt tcactcagga tgacattgac ctaagatact tcgctatcgc tcaggatgac
     7021 aaatcttcga tttgtattaa tattaccctt ttattctatt tttttaacaa tatctttgtt
     7081 gccgaaaata actttaaaca taaatacagg cagtactatt tgcctggtaa ttctctttgg
     7141 ctccttaata agcctgtaaa gccactccaa acctaagttt ataaatatgt ccggcgctct
     7201 tttcacgatt cccgccagac catccagtgt tccgccgttt cctataataa tattggcatt
     7261 tattaaatcc ttgttatatt ctatccactg ctcctgcttt ttagccccga atccgacaaa
     7321 cagaatatga ggcttatgcc tgtttatatc ttctataaca gctatttctt cctcgtggcc
     7381 gaattttccc aaatgagtac ccttaaaata tccgtgatgt gctcccacaa tcttaattcc
     7441 gggatatttc ttttgaacat tttgcatggc agcttcagcc actcccggtc ttccccccac
     7501 ggcatacagc ttaagacctt tttcatctgc cattttcagc aaatttatcg acaaatcata
     7561 acctgccacc ttttctttta aggggaattt tttaattttt ccagcataaa tcaaacccac
     7621 cccgtcggga acattgatgt ccgatttatt cattatattt aaaaattctt catccttttg
     7681 gcacatcatg attatctccg tatttggagt ataaattatg tacttttcat catttttaat
     7741 cttttgttct gccagtttta aaacttcatt catggatata ttattaattc taacacccat
     7801 tatacttaat ttatccattt gttatttcct tcagaatttt ataattaatg ttggttaatt
     7861 cccttaattt ttcaacgctt tttaacagca ttatcttttc ttcacgttcg ttatttattt
     7921 tatcgattat ttcatcataa attttatttt catcaatatt atcaatcgta caagctattt
     7981 tcatatttac gctgtttaca aagttatcaa ccttctggtc atatgatacg gcaacgatgg
     8041 gtacattggc agccgcagca aagataagcg aatgtaatct caccccgacc atcacatcca
     8101 tggttttaat aatgtcaaaa gcatcacctg ttgaaagcct ctccttataa tatacagcct
     8161 tactgcctaa taattcttca acctgggcaa taagctgcaa atcttcctta taataaaaag
     8221 gtatgaaatg gcattctatt ccatcatcta taagcctttg ggcaaccttg cttattttta
     8281 cgctgacatc cgaatccttc caattcctta aagaaaagca gactttcctt acactttgct
     8341 tttcatattc taaggaatcg tctttcctga tatttattac agggtctgtt gaaagaaaaa
     8401 tcttcctttc atccactcct atactttcca aaaattcctt ggaatgttta tctctcacgg
     8461 ttatgtagtc aaccaatgac aaggtgtttt taactgcctt aatgcttttt tcgtttatta
     8521 tgggaccgat accttgactg agcattacaa ccttattatt cataagtttt gccagtctta
     8581 ttaaaaataa ataataataa atgctcctct tgctggttac atcctgcaa
//
LOCUS       DAOPUE010000075         8594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8220_length_8594_cov_9.290432, whole genome shotgun sequence.
ACCESSION   DAOPUE010000075 DAOPUE010000000
VERSION     DAOPUE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_8594_cov_9.290432"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..682)
                     /locus_tag="PKH42_07235"
     CDS             complement(182..682)
                     /locus_tag="PKH42_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOA20262.1"
                     /translation="MKRNNRFITRTALIAAVYAALTYAFAWMSYEQIQFRISEVLVLF
                     AFIEPRYGLGLVLGCILANLASPFGVIDIAVGSFATFLAIVFIVKIRKLFGLNKKALI
                     IASLGPVITNALLVGAELTYLFNTPFLLNALYVGAGEFAVVVFIGTVVVNLIMKNNVL
                     VEKLSF"
     gene            complement(825..1214)
                     /gene="rpsI"
                     /locus_tag="PKH42_07240"
     CDS             complement(825..1214)
                     /gene="rpsI"
                     /locus_tag="PKH42_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOA20263.1"
                     /translation="MEAQYRGTGRRKKAIARVRLVPGTGKININERDLDDYIKYETLR
                     NLVREPLMITDTFGQYDVLVNVKGGGYTGQAGAIRHGISRALLQVDKELRPLLKKAGF
                     LTRDSRMKERKKYGLKKARRASQFSKR"
     gene            complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PKH42_07245"
     CDS             complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PKH42_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOA20264.1"
                     /translation="MKSFLAKPNEVERAWYVIDAEGKTLGRLATEVAKLLRGKHKAIY
                     TPHVDTGDFVIVVNAEKVVLTGKKLDQKLYRHHSLYPGGLKEVPYRNLFRSKPTQPVY
                     LAVKGMLPKNSLGRKMLKKLKVYAGPEHNNQAQKPQVYEF"
     gene            complement(1833..2573)
                     /gene="truA"
                     /locus_tag="PKH42_07250"
     CDS             complement(1833..2573)
                     /gene="truA"
                     /locus_tag="PKH42_07250"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOA20265.1"
                     /translation="MVRNIKLLIAYDGTAYHGWQTQLNKVTIQETIENALSIVMKQKI
                     DLTGSGRTDGGVHALGQVANFKADTKIPEDKIKIALNANLPPDIRILESVDVDMSFNA
                     RFDALDKTYMYQIYNDRVGNPFYSRYSCFIPQSLDIDKMKEALKLIKGTHDFKGFMAS
                     GSYVKTTVRTIYDTELIKEENLLKLYIKGDGFLYNMVRIIAGTLIDIGKGIKSIDCIE
                     DALINKNRKVLGQTAPPQGLFLMKVNYK"
     gene            complement(2567..3364)
                     /locus_tag="PKH42_07255"
     CDS             complement(2567..3364)
                     /locus_tag="PKH42_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOA20266.1"
                     /translation="MFKNLTIGQHYPVDSPIHNLDPRLKIIMTFIFIISLFLIESFTG
                     FIFVIIFLTVIITISKVPVKFVIKGLRPILFIILITFIINLLMTPGRVIYTIGVIKIT
                     EEGLRQAGFMAVRLTLLIMGTSMLTLTTSPIILTDGIESLLKPFKRFGLPAHELAMMM
                     TIALRFIPTLMEETEKIMKAQKSRGADFESGNIMSRAKNLVPLLVPLFISAFRRADEL
                     AMAMEARCYRGGENRTRMRQMKISKGDYAAAFVFTVYFAAVLIIKIW"
     gene            complement(3357..4217)
                     /locus_tag="PKH42_07260"
     CDS             complement(3357..4217)
                     /locus_tag="PKH42_07260"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOA20267.1"
                     /translation="MSIRTENIKYIYSEGTPFRTLALDNVSVTINQGEFVGIIGHTGS
                     GKSTLIQLFNGLELPTEGKVEVDGTIVGKDKKELKLIRQTVGLVFQYPEYQLFEETVA
                     KDVAFGPMNLNISQQEIDFRVKEALELVGFNYNKIKDVSPFELSGGQKRRVAIAGVLA
                     MKPQYLILDEPTAGLDPAGRNEIFNQIKKLHEESKFTVVLVSHSMEDIAKLVDRVIVL
                     SKGKIHMEGSPKEIYSQAEELEKIGLGIPQTASIVRELKNRGFNIKQDILTIEEAKEE
                     ILKEVRRRNV"
     assembly_gap    4240..4339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4340..4863)
                     /locus_tag="PKH42_07265"
     CDS             complement(<4340..4863)
                     /locus_tag="PKH42_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA20268.1"
                     /translation="MIEYIIKIDNVKFKYDKNSSYAVNGVSLNIKSGEFTAIIGRNGS
                     GKSTLAKLINSILLPTEGKIFVKGMDTSDDSKLWDIRQTAGMVFQNPDNQLVATIIEE
                     DIAFGPENLGVQPEEIRRRVDNALNTVGMYEYRKRPPHLLSGGQKQRIAIAGILALNS
                     ECIILDEPTAMLDPS"
     gene            5219..5686
                     /locus_tag="PKH42_07270"
     CDS             5219..5686
                     /locus_tag="PKH42_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20269.1"
                     /translation="MFCLRNKINICDFKPDIAQNLGNLSLTVRNRSSNEPVAFAEVSV
                     YYLTIYGVYGEGGEASMMVRHITDENGRIPVIELPVIDRINQPNNSYFVTVRHFRYYP
                     VNIMNIPIYPDITTEYNVMITPLTEQHPEYEFHITPELIRQRENLQNNYKTLF"
     gene            complement(5749..6870)
                     /locus_tag="PKH42_07275"
     CDS             complement(5749..6870)
                     /locus_tag="PKH42_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOA20270.1"
                     /translation="MIPGILYTVKSGDSLRDIADRFSIPFRELISTNKIINPELIYPG
                     VTLIIPQVRKPPIEVNGISYVFGNTDVPVLSNSMKYLTYLTPFSYIAREDGSLFWIND
                     QQAIQLAILNNVIPIMSVVNYSYYDTGTNAAHIILNDMTVMNELIDNIISIMIKKGYR
                     GVKFDFDNILPEDRDAYNNFLHVAGSRLNTEGFYISVALMPKFISPDYLAYDYEALGR
                     IVDFVLLKTYNWGLIEGPPAPIIPINDLRNVLDFALSIIPREKIFIGLPLYAKDWIIP
                     YVSGREAEIISAQTAIIRAQDNNAQIQYDEISQSPFFYYSDEQGRMHEVWFEDPRSLK
                     AKFDLVKEYGIRGITYWNLLYEFIQNWHLLEDNFIIVRD"
     gene            complement(6976..7536)
                     /locus_tag="PKH42_07280"
     CDS             complement(6976..7536)
                     /locus_tag="PKH42_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HOA20271.1"
                     /translation="MINKLSAKLSEGIGLRLNSSDNEKEIYAYSIEILISLLINFIIL
                     SITAYILKKQVELIVFIVFFSSLRSFAGGYHAKTHIECITLSFCLFVISAISSTYFIE
                     YGKTILFLGIIFSIIMVFVFAPTESENKPLSKKKFKKFRLISRILVIALSLTAICLYL
                     TKETMGHVYITAAVAMSFESISLLKH"
     gene            complement(7529..7663)
                     /locus_tag="PKH42_07285"
     CDS             complement(7529..7663)
                     /locus_tag="PKH42_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HOA20272.1"
                     /translation="MKKSLSLLSCIAMLLVTTAQASMGVTCILFINQPKVPQCLLEND
                     "
     gene            complement(7758..>8594)
                     /locus_tag="PKH42_07290"
     CDS             complement(7758..>8594)
                     /locus_tag="PKH42_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOA20273.1"
                     /translation="IIMVILAWMYGNIDVTNKSIGKTLLMLTLCISLWSIASITLINQ
                     VLKDMEEKHKNEMELQQINLERVYLTDVNAALEEIRILRHDMRGELALIHGYNELNQK
                     DKIRLHIEKKLHEMDVQLIPQIDSENIITSFLNFKIKEAESKNINVELTSNINADEEI
                     YIDKGDICRIINNIMNNAIEALQDCDTKNMKLFIGTAGGYLIIKSENPYKGSLLTEGK
                     KIITIKKDKTKHGYGLKSINSIAEKYNGFMSIRYEDNIFKIDVEMLNNEMYKNATVEF
                     TV"
BASE COUNT         2530 a         1684 c         1256 g         3024 t
ORIGIN      
        1 tcaattgcaa acacaacaaa tcgaaaatca ttcatttgtg taaatttggg gatagctcaa
       61 ttttttctca tccggtgcaa gtattcggtg cctgaagtat tcggtgccag ggaaacctcc
      121 atattttcgg aggtttccct ggcaccggat aaatggcacc ggataaagaa actgtcctcg
      181 tttagaatga aagtttttca accaggacat tgtttttcat tattaaattt acaacgacag
      241 tgcctatgaa aacaactacc gcaaactcgc ctgctccaac atataatgca ttcaataaaa
      301 agggagtgtt aaacaaataa gttaattctg ctcctaccag taaagcgttt gtaattacag
      361 gtcccaaact tgctataatc agcgcttttt tattcaatcc gaataatttt cttatcttta
      421 caatgaatac tattgccaaa aatgttgcaa atgagcctac cgcaatgtcg attaccccaa
      481 aggggctcgc taagttagcc aatatgcatc ccaatactaa gccgagacca tatcttggct
      541 ctataaatgc aaacaagacc aatacttctg atattctaaa ctgtatctgc tcgtagctca
      601 tccatgcaaa ggcataggtc aaagcagcat aaaccgccgc tataagagca gtgcgtgtta
      661 taaaccgatt gtttcttttc attaaaaaaa ctcctttcga atatattctc ggagtcacta
      721 aaaaaccttg attttttaag tgggtgccaa gaaaacacta tagattaagt ttttaaggga
      781 cggtcctttt tgttttctta aaaaagaatc gtccccaaat atttttatct cttgctgaac
      841 tgtgaagctc ttcttgcttt tttcaaaccg tatttctttc tttctttcat tctggaatct
      901 cttgttaaga atccggcttt ttttagcaag ggtcttaatt ccttgtctac ctgtaaaagc
      961 gctcttgata taccatgtct gattgctccg gcctgacctg tatatcctcc gccctttaca
     1021 tttaccagta catcatattg accgaatgta tcagtaatca ttaaaggctc tcttacaagg
     1081 tttcttaaag tttcatattt gatataatca tctaaatctc tttcatttat gtttattttc
     1141 cctgtcccgg gaactaatct tactcttgct atcgcttttt ttcttctgcc tgttcctctg
     1201 tactgtgctt ccatcgtatt cctcctctcc cattaaaact cgtatacttg cggtttttga
     1261 gcctgattat tgtgctcggg acctgcgtat acttttaatt tctttaacat ttttcttcca
     1321 aggctgttct ttggaagcat tccctttact gccagataaa caggctgtgt tggtttactt
     1381 cggaataaat tcctgtaagg tacttccttc aaaccacccg gatatagaga gtggtgtctg
     1441 tataattttt gatctaattt tttacctgtc aatacaacct tttcagcatt tactactatt
     1501 acaaagtctc ctgtatcaac atgcggtgta tatatagctt tatgtttacc tctaagaagt
     1561 ttggcaactt ctgttgcaag tctacctaat gtcttacctt cggcatctat gacataccat
     1621 gccctttcaa cttcattagg tttagctaag aagcttttca ttttctttcc tcctgtaatt
     1681 ctactgtatt aaattatctg tttatctatt caaaaaacct aactccgggg cattggaacg
     1741 gtttttttct cttttcatac ctaaagtatt ttatattaaa taaattataa tgtcaagcac
     1801 ttttcaggca ctttttcatt aatatcccgg ctttatttat aatttacctt cattaaaaac
     1861 aacccttggg gaggagccgt ctgccctaat actttcctgt ttttattaat taaagcatct
     1921 tctatgcaat caatgctttt tattcctttc ccaatatcaa ttaaggttcc tgcaataatt
     1981 cttaccatat tgtacaaaaa tccatcgccc ttaatataga gttttaacaa attttcctct
     2041 tttataagct cggtatcgta aatagttctc accgttgttt ttacatagga gcctgaagcc
     2101 ataaatcctt tgaaatcatg ggttccctta atcaatttaa gggcttcttt cattttatca
     2161 atatccaaag actgcgggat aaaacatgaa taccttgaat aaaacggatt cccgacccta
     2221 tcattgtaaa tttgatacat atatgtttta tcaagtgcat caaatcttgc attaaaactc
     2281 atatcaacat caacagattc caatatcctt atatccgggg gcaaatttgc attaagcgca
     2341 attttaatct tgtcttcggg tattttggtg tctgctttga aatttgcaac ctgccccaag
     2401 gcgtgaacgc cgccgtcagt ccttcctgag cccgttaaat caattttttg cttcattact
     2461 atacttaatg cattttcaat cgtttcttga attgtaacct tatttaattg ggtttgccac
     2521 ccgtgataag cagtaccgtc ataggcaatt aaaagtttaa tatttcttac catatcttaa
     2581 ttattaatac tgctgcaaaa taaaccgtaa aaacaaatgc tgcagcataa tcaccctttg
     2641 atattttcat ttgcctcatt cttgttctgt tctctcctcc acgatagcat cttgcttcca
     2701 tcgccattgc caattcatcg gcacgtctga atgcacttat gaaaagcgga accaagagag
     2761 gaactaaatt ttttgccctg ctcataatat tgccgctttc aaaatcagca cctcttgatt
     2821 tctgtgcctt cattattttt tctgtttctt ccataagggt aggaataaag cgaagagcaa
     2881 ttgtcatcat cattgccagt tcatgtgcag gaaggccgaa tcttttaaac ggctttaaca
     2941 gactttcgat accgtcagtc aatataatgg gcgacgttgt cagtgtcagc atagatgttc
     3001 ccattattag aagagtaagt cttacagcca taaatcctgc ctgcctcaaa ccttcttcag
     3061 taatctttat tactccgatg gtatatataa ctcttcccgg cgtcatcaaa agattgataa
     3121 taaatgtaat taatatgatg aataatatgg gtcttaatcc tttgattaca aatttaacgg
     3181 gtacttttga aatagtaata ataacggtca aaaatataat aacaaatatg aaccctgtaa
     3241 agctttcaat caaaaataat gaaattataa aaataaatgt catgattatt ttaagcctgg
     3301 ggtcaaggtt gtgtatggga gaatcaacag gataatgctg tccgatagtt aaattcttaa
     3361 acatttcttc tcctcacttc ctttaatatt tcttcttttg cttcttcaat tgtgagtata
     3421 tcctgtttaa tattaaatcc tctattctta agctccctta cgatagaagc tgtctgagga
     3481 attccaaggc ctattttttc caattcttca gcttgtgaat aaatttcttt tggactgccc
     3541 tccatatgta ttttcccctt ggataaaact ataactctgt ccaccagttt tgctatatcc
     3601 tccatactgt gtgataccaa cacaacggta aatttagact cctcatgcaa tttttttatt
     3661 tgattaaata tttcattcct gcctgcaggg tcgagaccgg cagtgggctc atctaatata
     3721 aggtactgcg gtttcattgc aagtacgcct gcaattgcaa ctcttctttt ttgacctccc
     3781 gataactcga agggagatac atcttttatt ttattataat taaaaccgac taattccaat
     3841 gcctctttta cccgaaaatc aatttcttgc tgggagatat ttaaattcat tggtccaaag
     3901 gctacatcct ttgcaactgt ttcttcaaaa agctggtatt cggggtattg aaatactaat
     3961 cctactgttt gacgaatcaa ctttaattct ttcttatctt tgccgactat tgtgccgtca
     4021 acttcaactt taccttcagt gggaagctcg aggccgttaa aaagctgtat caaagttgat
     4081 ttgccggagc cggtatgtcc gattattccc acaaattcgc cttggtttat cgtaacgctc
     4141 acattatcca atgccaaggt tctaaacgga gtgccttcac tgtatatata ttttatgttt
     4201 tctgttctta ttgacataat atgtccacca attcctttan nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnng aagggtctaa cattgcagta ggctcatcca atattatgca
     4381 ctccgaattt aaagctaata tacctgctat ggcaattctt tgtttttgtc ctccagataa
     4441 gaggtgaggg ggtctttttc tgtattcata catgcctact gtatttaagg cattatcaac
     4501 ccttcttcta atttcttcag gttgcacgcc taaattttcg ggaccgaatg caatgtcttc
     4561 ctcaatgatg gttgccacta actgattatc aggattttgg aacaccatac cggctgtctg
     4621 tcttatatcc caaagtttag aatcatctga ggtatccatt cctttaacaa aaattttccc
     4681 ttcggtagga agtaaaattg aatttattaa tttagccagc gttgatttcc cagagccatt
     4741 gcggcctata attgcagtaa actctccgct ttttatattc agggaaactc cgtttactgc
     4801 ataagaggaa tttttatcat atttaaattt tacattgtct attttgatta tgtattctat
     4861 cattttacac ccttatttga atttttgcgt taaaagctgc ataagaataa tatcatcatt
     4921 cgcattattt tacaacagta atttacacta atgttacggt tatcggtttc tttggaatta
     4981 caaaaaaata aaaggctgag caaagtatat cactctgcgc accttttatt ttattcgttt
     5041 aataataatt catcatcctg ttttcacaca atatttatga taacaaaaga gcgcctttgg
     5101 aattttctaa aattattgta taattatgcg cccactccaa aagcactgaa cttgttaata
     5161 taatcaataa ttcataattt tcatataata ggttataacc atatgggagg aaatataaat
     5221 gttttgttta agaaataaaa taaatatatg tgactttaaa cctgacattg cccaaaactt
     5281 aggtaattta tccttaactg tgcgtaacag atcaagcaat gagcctgttg cttttgctga
     5341 agtaagtgtt tattatttga ctatatatgg agtttatggc gagggaggag aagcaagtat
     5401 gatggtaagg cacataacgg atgaaaacgg cagaatccct gttattgaac tgcctgtcat
     5461 tgacagaatc aatcagccta ataactcgta ctttgttacc gtaaggcatt tcagatatta
     5521 ccctgttaat ataatgaata taccgatata ccctgatatt accaccgaat acaatgtgat
     5581 gataacaccg ctgaccgaac aacatcctga atatgaattt catataactc cggaattgat
     5641 acgacaaagg gagaatttgc aaaacaatta caaaacactg ttttaacgag gcgtaatcac
     5701 ccgcctcgtt aaaatttata tactcagctc tttcatttat atgccatact aatctcttac
     5761 tattataaaa ttatcttcaa gcaagtgcca gttttgaata aattcataca gcaaattcca
     5821 atatgttata ccccttatac catattcctt gacaagatcg aatttcgcct taagacttct
     5881 cgggtcctca aaccaaacct catgcatcct tccctgctca tccgaataat agaaaaacgg
     5941 agattgactt atttcatcat actgtatttg tgcattatta tcctgtgctc ttattattgc
     6001 tgtttgagca cttataattt cagcttccct gccgcttaca tagggtataa tccaatcctt
     6061 ggcgtaaagt ggcagtccga taaaaatttt ttctcttgga attatagata aggcaaaatc
     6121 taaaacattt cttaaatcat ttataggtat aatcggtgca ggcggacctt caatcaatcc
     6181 ccaattatag gttttcaata atacaaaatc gacaatcctt ccaagtgcct cataatcata
     6241 tgccaaataa tccggactga tgaacttagg cattaaggca acagaaatat aaaaaccttc
     6301 cgtatttaac ctgcttccgg caacgtgtaa aaagttattg tatgcatctc tgtcttcagg
     6361 cagtatatta tcaaagtcaa actttactcc tctgtatccc ttttttatca taatgctgat
     6421 aatattatct ataagttcat tcattacagt catatcgtta agaataatat gagcagcatt
     6481 tgttcctgta tcataatatg aataatttac taccgacata ataggtataa cattatttaa
     6541 tattgctaac tggattgctt gttggtcatt aatccaaaac agacttccat cttctcttgc
     6601 tatatacgag aacggtgtta aataagttaa atatttcatc gagttgctca atacgggaac
     6661 gtctgtattg ccaaagacat agctgattcc gtttacttca atcggcggtt tgcgcacttg
     6721 aggtattatt aatgttactc ccggataaat caattccgga ttaattatct tgttagtcga
     6781 tataagttct ctaaacggaa tagaaaatct atcggctata tccctcaagg agtcaccgga
     6841 tttgaccgtg taaagaatgc ccggtatcaa atgattaccc tcctgtattt taatatactg
     6901 tattatattc ttaacaaaaa aattggggac ggtttttctc aaaagaaaaa actgtccccg
     6961 tatctcttat gttacttaat gcttcaataa acttattgat tcaaaagaca tcgccactgc
     7021 tgctgttata tatacatgcc ccatagtttc tttcgttaaa tataaacaaa ttgctgtcaa
     7081 agataaagct attactaata tcctgctgat taatctaaac ttcttaaact tttttttgct
     7141 taaaggcttg ttttcagatt cagtcggagc aaaaacaaat accattatta ttgaaaatat
     7201 aattcccaaa aataatattg tttttccgta ctcaataaaa taagtactgc ttatggctga
     7261 tattacaaat aaacaaaacg ataaggttat acactcgata tgagttttcg catgataccc
     7321 tccggcgaaa cttctaaggc ttgagaaaaa tactataaat acaataagtt caacctgctt
     7381 tttcaaaata tatgccgtta tggaaaggat aatgaaattt attaataaag atatcagtat
     7441 ttcaatgcta tatgcgtata tttctttttc attgtctgaa gaattgagcc taagcccaat
     7501 tccttcagac aattttgccg ataatttatt aatcattttc cagcaggcat tgaggtactt
     7561 tcggctgatt aataaaaagt atgcatgtca ctcccataga tgcttgagct gttgttacca
     7621 aaagcattgc aatacatgat aataatgata aacttttctt cattataatc cccctttcct
     7681 ttaacaacat aataagacaa aaggtggcat aattcaacat tttttcctga aaatgcctat
     7741 tcggttgttt ccattgctca aacggtaaat tcgactgttg cgtttttata catttcattg
     7801 tttaacattt ctacatctat tttaaaaata ttatcttcat accttatact cataaaaccg
     7861 ttatactttt cagctatact gttaatactt tttaagccgt agccatgttt agttttatcc
     7921 tttttaattg taataatttt tttgccttcg gttaataagc tgcccttata aggattttct
     7981 gattttataa ttaaatagcc tcccgctgtc ccgataaaga gcttcatatt ttttgtatca
     8041 caatcttgta atgcttcaat tgcattgttc attatattgt ttataatcct gcaaatatct
     8101 cccttgtcga tgtatatttc ttcatcggca ttgatattgc ttgttaactc aacgtttata
     8161 ttcttggact ctgcttcctt tattttgaaa tttaagaatg aagttataat attctcgctg
     8221 tctatttgag gaataagctg tacatccatt tcatgaagtt tcttttctat atgtaatctg
     8281 attttatcct tttggtttaa ttcattgtaa ccatgtatta gtgctaactc tccgcgcata
     8341 tcatgtctca gaattcttat ttcttccaag gcagcattta catccgttaa ataaactctt
     8401 tccaaattga tttgctgaag ctccatttcg tttttatgtt tttcttccat gtcttttaga
     8461 acttggttta ttaaagttat agaagcaata gaccataatg aaatgcataa tgtcagcatc
     8521 aataaggttt tacctatact tttattggta acgtcaatgt ttccatacat ccatgcaaga
     8581 ataaccataa ttat
//
LOCUS       DAOPUE010000076         8522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8304_length_8522_cov_9.685367, whole genome shotgun sequence.
ACCESSION   DAOPUE010000076 DAOPUE010000000
VERSION     DAOPUE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_8522_cov_9.685367"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1629)
                     /locus_tag="PKH42_07295"
     CDS             complement(28..1629)
                     /locus_tag="PKH42_07295"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOA20274.1"
                     /translation="MPKYIFVTGGVVSSLGKGITAASLGRLLKSRGLKVTLQKFDPYL
                     NVDPGMMSPYQHGEVYVTDDGAETDLDLGHYERIVDIDLTQYSSITSGKIYWAVLNKE
                     RRGDYHGGTVQVIPHVTNEIKCMLKRIASVEDVDIVITEIGGTVGDIESQPFLEAIRQ
                     IRYEVGRENVLYIHVTLLPYLSMAGELKTKPTQHSVKELLSIGIQPDILVCRSEKEIP
                     EELKAKIALFCNVEKQNVIQNEDTNSIYDIPLMLEKEGLAKLVCDHFNIKCTEPDLIE
                     WTDMVERSKNPKNSVKIALVGKYVELKDAYLSVAEALKHAGFDNSCDVDISWIHSDHV
                     NENNYKQILKDCDGILVPGGFGDRGIEGKILAAKYARENRKPYLGIGLGMQTAIIEFA
                     RSVLGYADADSFEFNAESGHKIIDVTDGQENIEKNGVAMRLGSYACKLVDGTKTKEAY
                     EGADMIHERHRHRYEFNNKFRDVCQENGLIISGISPDNKLVEIVELKDHPWFVGCQFH
                     PEFKSRPTRSHPLFKNLIKASIEFK"
     gene            2032..3579
                     /locus_tag="PKH42_07300"
     CDS             2032..3579
                     /locus_tag="PKH42_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOA20275.1"
                     /translation="MVKLFNDTFNINEIIDSNSTEVLLESEFGIADDMPDIEKLVTAE
                     GKAKLNNVTVTTDMIIVDGRLIYNIIYRSNNENYTIHSMSGEIPFREEIAAEGVTEDM
                     EALSNIFIDYIDADKLSERNFLVKAVLILDTDIYMKRPVTYVSNLESDGSIQAKAKNI
                     KYTDTIAEISEEININDAVELSKGSGEIDKIIKSEADVYITNIDELNDKMLIEGICKV
                     GFMFIEDDELHTIGYVSEEFPFTHYLELASSSENMLKDIKIAVNDISYSITENFDDEK
                     KLIEFALPFTVNASFYDSVEKNIIMDCYSTDYELELQSEKVKLSSLKNLTNEVVKYEN
                     SFEVLTGSIKDIYSVDVSPKVSEKRISDNKYIVDGFLDVNLLYLNGDINKIDKAFASL
                     PFTAGFPIDEDEALFEIHSDITTHKCSAYRKGNNSVVLNCDINVALKFKDDDEITVIR
                     DIAEKTPVDRSKMPSLIFRVVQSGETLWDIAKNYNLSINYLKELNDIPEGNVLTPGTK
                     IIIARMI"
     gene            complement(3650..4969)
                     /gene="dnaB"
                     /locus_tag="PKH42_07305"
     CDS             complement(3650..4969)
                     /gene="dnaB"
                     /locus_tag="PKH42_07305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOA20276.1"
                     /translation="MNLGKIPPHNAEAEQTVLASCMIDHTAVEKVVNLLSYDDFYYEA
                     NKEIYDSIKQIHMQNIPVDAVTVFEELKKRGKIDYIGGFEYLATLTENIITSKNVEAY
                     CNIIREKSTLRKLIGASQEIIEKSYKEDDEVQRIIELAEQRIFSISQNRSINTFSEIK
                     EVLMTVFNQLEERAKSGGGITGLATGYTDLDRMTSGLQKSDLILLAARPSMGKTALAL
                     NIAMNVVKNGASVALFSLEMSEEQYVQRIISQEAMVDSTKLRTGNLDDDDWTRLINTM
                     SLISNYKIYIDDTPSVTLFEMMSKCRRLKIEKGLDLIVVDYLQLMSDGGRTDNRQQEI
                     SNISRGLKALARELDCPVLALSQLSRAPEIRNDHRPVMSDLRESGAIEQDADVVMMLY
                     RDEYYNKEESEKKGITDVIITKQRNGPVGTVELAWIGQYTKFGNIQK"
     gene            complement(4991..5437)
                     /gene="rplI"
                     /locus_tag="PKH42_07310"
     CDS             complement(4991..5437)
                     /gene="rplI"
                     /locus_tag="PKH42_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOA20277.1"
                     /translation="MKVILLEDVKNVGQKGQIINAKDGYARNFLFPKKLAIEATEANL
                     KNLENQKRKKEEQEKKILEEAKVLEEELLKKTIVIKTKAGENGKLFGSITTKEISDVL
                     EKEHKILVDKKKFDLEEPIKSVGEYYVKIKLHPTVTAKLKVIVTEK"
     gene            complement(5434..7437)
                     /locus_tag="PKH42_07315"
     CDS             complement(5434..7437)
                     /locus_tag="PKH42_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOA20278.1"
                     /translation="MDKKTPVFLKDDSFIYFVVIVLLIIIFLIESMFIYAASAGLVLV
                     IITAFTLRKQILRSRELKNYLINYTKNLENLSINSFYYLPLPICIISLNGKVFWYNNK
                     FKELFPENIDIIENVDEFVGGFPLNSMDGNKEGTISNIEVPETGRSFNVLYYELEEGR
                     FGEGKSYVCYWNENTAFVNLRNKYNDERPITMLIQIDNYEEISEKLELGEKSSMTAQT
                     EKILNSYAAEMNGFVLKYDNYKFLMMIENKYLETLEAKKFSMLDDVKSIKGTNDFNFT
                     LSIGTGAAAKNLTQLMEYSKGALDVALGRGGDQAVVKRVNSVKFYGGKSKAVEKRTKV
                     RARIISYALRQIIDQSSNVIIMGHRVGDMDSLGSAIGIYSIARSRGKKSYIVLNNVNH
                     ALKNMYGRMKKEESHYIESIITTEKALELADQNTLIVVLDTHRKSYTEAPELIDKIDK
                     VVLIDHHRRSEEYIENNLLDYIEPYASSTCELVAELIQYMEDHIKLTKFEADALMAGI
                     VVDTNSFTFKTGVRTFEAAAFLKRRGADNVEVKELFNESIEFMKKKTEIIERSEIVFD
                     DVAVSYIDEPTEDSNVAAAQCADELLTIKDVNMSFVLVRNKEYINISGRSVGDMNVQV
                     ILERLGGGGHLNMAGAQVQTDDMEEAKARLYEAIAQYKKEIKP"
     gene            complement(7452..8414)
                     /locus_tag="PKH42_07320"
     CDS             complement(7452..8414)
                     /locus_tag="PKH42_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOA20279.1"
                     /translation="MNRNLKTSNITESAMITGILVIIAYLSSFITLLMFFYPTPAIIL
                     GKRKGLKYSVLSLIASDLIISMLLGLQTGLVFLILYTPLALALTYGVCRNEEANKTIL
                     FGSAAYMISFVLYILLLNYIMGINFIERIAEIYEQSFETTRGLFNNIPDQLRTEQIEQ
                     YINDIEKMAPMMNYIVTNVFPAVLIVASVVTSYINYMVASKFAVRFSINVKQHEGIAY
                     FSFPRNFMISMAGLLLLSFLLRLLNINVGIIQMNLFIIVFMAMLLQGVAVLKFFVDKS
                     RMGKFARNLLLVFIVLMSINFSIAYAVIGLIDLTVNLRKINRAV"
BASE COUNT         2590 a         1668 c         1310 g         2954 t
ORIGIN      
        1 aaaaatataa gacagtgagt cactgtctta tttaaattct atcgaagctt ttattaaatt
       61 cttgaacaaa ggatggcttc ttgtaggtct tgatttgaat tccggatgga actgacaacc
      121 tacaaaccaa ggatggtcct ttagctctac tatttcaacc aatttgttgt ccggtgaaat
      181 tcctgaaatt ataagtccgt tctcctgaca cacgtccctg aatttattgt taaactcata
      241 tctgtggcga tgcctttcat gtatcatgtc agcaccttca tatgcttctt ttgtcttagt
      301 gccgtcaaca agcttgcacg cgtagcttcc aagcctcatc gctacgccat ttttttcgat
      361 attttcctgc ccatccgtta catcaataat tttatgaccg gattcagcat taaattcaaa
      421 gctgtcggca tcagcatatc ctaagactga cctggcaaat tcaataattg ctgtttgcat
      481 gccaagtcct attcctaaat agggctttct attttctctt gcatattttg ccgcaagaat
      541 tttaccttct attcccctgt cgccaaaacc gccgggaact aatattccgt cgcaatcctt
      601 aagtatttgt ttatagttgt tttcattgac atgatctgag tgtatccaag aaatatcaac
      661 atcgcaagaa ttatcaaatc ctgcatgttt gagtgcttcc gcaactgaca aatatgcatc
      721 cttaagctct acatacttgc ccacaagtgc tattttaaca ctattttttg gatttttaga
      781 gcgctcaacc atatcagtcc actctattaa atccggctcg gtacatttta tgttgaaatg
      841 gtcgcagact aattttgcaa gaccttcctt ttccaacatt aaaggaatat catatatgga
      901 atttgtatct tcattttgaa taacattttg tttttcaaca ttacaaaata atgcaatttt
      961 tgccttcagt tcttcaggta tttctttttc gcttctgcac accaatatat ccggctgaat
     1021 acctatgctt aaaagctctt taacgctgtg ctgtgtcggt tttgttttca gttctcccgc
     1081 cattgacaaa taaggaagca aggtaacatg tatatacaat acattttctc ttcctacctc
     1141 gtaacgaatt tgacgaatag cttccaagaa gggctggctt tcaatatctc caacagttcc
     1201 accgatttcg gttataacta tatcaacatc ctcaactgag gcaattcttt ttaacataca
     1261 tttgatttca ttggttacat gaggaataac ctgcactgtt cctccatggt aatctcctct
     1321 tctttcctta ttcaatacag cccagtatat tttccccgat gtgatgctgc tgtattgagt
     1381 taaatctata tcaactattc tttcatagtg tcctaaatct aaatctgttt cggctccgtc
     1441 gtcagttaca tacacttctc catgctgata agggctcatc attcccgggt ccacgtttaa
     1501 ataagggtca aatttctgca atgttacttt aagtcctctg ctttttaaaa gccttcccaa
     1561 tgaagctgct gttattcctt ttcccaatga agaaacaact cccccggtta caaaaatata
     1621 tttcggcata ttttcctcct gataactatt attccaataa ttttaattaa tataagattc
     1681 cctgccccta tttatttaag gtatggggac acacctccca tctgatcaac cggcaggagg
     1741 aatgtcccca attgcttcat accttgttaa cataaaaaag agacaaaaag gggttttttt
     1801 tttagtctct attaattcga atctctttgc aattatttaa ttaatttggc aatttaaaga
     1861 attgtcatca aattactaaa tcattttata ttaaataaat aaaaaggtca atccttttta
     1921 atactttttg aaaaaatttt atttataagt tgtactattg aaatttaaac ggcatataca
     1981 tttataaaag aataatgcgt aattccgtat taacttaaag gaggatttat tttggttaaa
     2041 ttatttaatg atacatttaa tattaatgaa attattgatt caaattcaac tgaagtattg
     2101 ttggaaagcg agtttggaat tgctgatgac atgccggata ttgaaaagct cgtaaccgcg
     2161 gaaggaaaag caaagcttaa caatgttacg gtaacaacag atatgataat agttgatggc
     2221 aggctgatat ataatattat ttatcgttca aacaatgaga attatacaat acattccatg
     2281 tcaggcgaaa tacctttcag agaagaaatt gccgcagaag gcgtaactga agacatggaa
     2341 gcattatcaa atatttttat agattatatt gatgccgata agctgtcaga aaggaatttt
     2401 cttgttaaag cagtattaat actggatact gatatctaca tgaaaagacc tgttacctat
     2461 gtttcaaatt tagaatctga cggaagcatc caggctaagg ctaaaaatat taaatatacg
     2521 gatacaatag cagaaatatc tgaagaaata aacataaatg atgcggtaga acttagcaag
     2581 ggttcaggcg aaatagataa aattattaaa tcagaagctg atgtttacat aacaaatata
     2641 gatgaattaa atgataaaat gctcatagag ggaatatgca aggttggatt tatgtttatt
     2701 gaagacgatg agcttcatac tatcggatat gtatctgaag aattcccctt tactcattac
     2761 ctagagcttg cttcttcaag tgaaaatatg ttaaaggaca taaaaattgc agtaaatgat
     2821 atatcttatt caataactga aaattttgat gatgagaaaa aacttataga attcgcactg
     2881 ccatttacgg taaatgcatc attctatgat tctgttgaaa aaaacattat catggactgc
     2941 tattcgactg attatgagtt ggagctgcaa tcagaaaaag taaaattgtc atcgcttaag
     3001 aatttaacta atgaagttgt aaaatatgaa aattcttttg aagtgcttac aggctcaata
     3061 aaggatatct attcggtaga tgtaagcccg aaggtttctg aaaagagaat atctgacaac
     3121 aaatacattg tagatggatt tttggatgta aatttgcttt atttaaacgg agacataaat
     3181 aaaatagaca aagcatttgc ctcacttccg tttaccgccg gcttcccgat agatgaagat
     3241 gaagcattat ttgaaataca ttctgatatt acaactcata aatgcagtgc ctacagaaaa
     3301 ggaaacaact cggtagtttt aaactgcgat attaatgtag cattaaaatt caaagacgat
     3361 gacgaaatta ccgtaatcag ggatattgcc gaaaaaaccc ctgtagacag gagtaaaatg
     3421 ccaagcctga ttttcagagt ggttcagtcc ggagaaactc tgtgggatat tgcaaagaat
     3481 tataatttat caataaacta cctgaaagag cttaatgata ttcccgaagg caatgtactt
     3541 acaccgggaa caaaaataat aattgcaaga atgatataga tatgagattc ttcgctgacg
     3601 ttcaggaata cagtatgtta tcctgagcgc agcgatgaat ctttttttgc tacttttgta
     3661 tatttccaaa ctttgtatac tgacctatcc atgccagctc aacggttcct acgggaccgt
     3721 ttctttgttt ggttatgatt acatccgtta tacctttttt ctcggattct tccttgttgt
     3781 aatattcatc tctgtaaagc atcatcacta catcggcatc ctgctcaata gccccggatt
     3841 ctctcaagtc agacataaca ggtctgtggt catttctaat ctccggtgca cgggaaagct
     3901 gagacagggc caataccggg caatcaagct ccctggcaag agccttcagt cctcgcgata
     3961 tattggaaat ctcctgctgc ctgttgtccg tccgtcctcc gtcggacatg agctgcaagt
     4021 agtcaactac aattaaatct aaaccttttt cgattttaag cctcctgcat ttagacatca
     4081 tttcgaataa tgtaacggat ggcgtgtcat caatatatat tttgtaattt gaaatcaagc
     4141 tcatggtatt aatgagcctt gtccaatcat cgtcgtctaa attgcctgtc ctgagttttg
     4201 tgctgtcaac catggcttct tggcttataa ttctttgaac gtattgctcc tctgacattt
     4261 caagactgaa aagagcaaca gaagcgccgt tcttaacaac attcatggca atattcaagg
     4321 caagggcggt ttttcccatt gatggtcttg ctgcaagcag tatcaaatca gatttctgaa
     4381 gtcccgaagt cattctgtct aagtcagtgt agcctgttgc aagacctgta atgccgccgc
     4441 cgctcttggc tctttcttca agctggttaa atactgtcat aagaacttct ttaatttcag
     4501 aaaatgtatt gatgcttctg ttttgtgaaa tggaaaaaat tctttgctca gccaattcta
     4561 tgattctttg aacttcatca tcttccttat agctcttctc tataatttct tgagaagcgc
     4621 caataagttt acgcaaggtt gatttttctc ttattatatt gcagtaagct tcaacatttt
     4681 ttgaagttat tatattttcg gttagtgtcg ctaaatattc aaatccgcct atatagtcta
     4741 ttttgcctct ttttttcagc tcctcaaaaa cagtgaccgc atcaacaggt atattttgca
     4801 tgtggatttg tttaatgctg tcatatattt ctttgtttgc ttcataataa aaatcgtcat
     4861 aactcaacaa attaactact ttttcgacag ctgtgtggtc aatcatacag gaagctaaga
     4921 cagtctgctc agcttccgca ttatgaggag gaatttttcc caaattaatt atatcagaca
     4981 tttttacccc ttacttttct gttactatta cttttaattt agcagttacc gtcggatgaa
     5041 gttttatttt cacatagtat tcaccaacag atttaatagg ctcttctaag tcaaatttct
     5101 ttttatcgac taaaatttta tgctccttct ctaaaacatc cgatatttct tttgttgtaa
     5161 tagagccgaa aagctttccg ttttcaccgg cctttgtttt tataacaatg gttttcttaa
     5221 gcagttcttc ttcaagaact ttggcttctt ccaagatttt cttttcctgt tcttcttttt
     5281 tccttttttg attttctaag ttttttaaat tagcttcggt agcttctatg gctaattttt
     5341 tgggaaaaag gaaattgcgg gcatatccgt ccttagcatt aattatttga cccttttgac
     5401 cgacattttt aacatcttcc aatagtatta ctttcatggt ttaatctcct ttttatactg
     5461 agctatggct tcatacagtc tggcttttgc ttcttccatg tcatcagttt gtacttgagc
     5521 tcctgccatg ttcaaatggc ctcctcctcc aaggcgttcc aaaattacct gcacattcat
     5581 atcccctacg gagcggccgc ttatatttat atattctttg tttcttacaa gcacaaaact
     5641 catatttaca tccttaatcg taagcagctc atctgcacat tgggctgctg ccacgttgga
     5701 atcttcagtg ggctcatcaa tatatgatac tgctacatca tcaaatacaa tttcacttct
     5761 ttcaataatt tctgtctttt tcttcataaa ttcgatgctt tcattgaaca attccttaac
     5821 ttcaacattg tcggcacctc ttctcttcaa aaaggctgct gcttcaaaag ttcttacgcc
     5881 tgttttgaat gtaaatgaat ttgtatccac cactattcct gccataagag catcggcttc
     5941 aaattttgta agcttaatgt ggtcttccat gtattgaatc aattcagcca caagctcaca
     6001 tgttgatgaa gcatagggtt caatgtaatc caataaattg ttttcaatat attcttcact
     6061 tctcctatga tggtctatca atacaacctt atcaatttta tcaataagct cgggagcttc
     6121 ggtataactt ttcctgtgcg tatccaaaac aacgatgagc gtattttggt cggcaagttc
     6181 caatgccttt tcagttgtta tgatggattc aatataatgg ctttcttctt ttttcattct
     6241 tccatacata tttttcaaag catgattcac attattcagc actatgtatg atttctttcc
     6301 cctgcttctg gctatgctgt aaattcctat ggccgagccg aggctatcca tatctcccac
     6361 tctgtgcccc ataataatca cattagagct ttggtcgatt atttgcctca atgcataaga
     6421 tataatcctg gccctgactt ttgtcctttt ttcaacggcc ttgcttttgc cgccgtaaaa
     6481 tttaacggaa ttcacccttt taacaactgc ttggtcgcct cctcttccca aagctacatc
     6541 aagcgctcct ttggaatact ccataagctg agttaaattc ttagccgctg caccggtacc
     6601 tatgctgagc gtgaagttaa aatcattggt accctttatt gatttaacat catcaagcat
     6661 ggaaaatttc tttgcttcca atgtttcaag atatttgttt tcaatcatca tcaaaaattt
     6721 ataattgtca tatttcagca caaacccatt catttcagca gcataagagt taagaatttt
     6781 ttctgtctgt gcagtcatgg aagatttttc acccagctct aatttttccg atatttcttc
     6841 ataattgtct atctgaatca gcatagtaat aggccgttca tcattatatt tattcctcaa
     6901 gttgacaaat gccgtattct cattccaata gcaaacatag gattttcctt ctccgaatcg
     6961 cccttcttca agttcataat acaatacatt gaaacttctg cccgtttcag gaacctctat
     7021 attgcttata gtgccttcct tgtttccatc cattgagttt aacgggaatc ctccgacaaa
     7081 ttcatctaca ttttcaatta tatctatatt ttcaggaaac agctctttaa acttattgtt
     7141 ataccagaat actttgccgt ttaaactgat tatgcatatg ggcaagggta aataataaaa
     7201 agaatttatt gataagtttt ctaaattctt cgtgtaatta atcagataat tcttaagttc
     7261 cctactgcgc agaatttgtt ttcttaatgt gaaagctgtt attataacta agactaaacc
     7321 agctgaggca gcataaatga acatactttc aattagaaaa attattatca ataatactat
     7381 aactacaaaa tatataaaac tgtcatcttt caagaatacc ggagttttct tatccatatt
     7441 gcctccttca actatacggc tctgttaatt tttcttaaat ttacggttaa atcaatcaat
     7501 ccgattactg cataagcaat gctgaaattt atgctcataa gtacaatgaa aaccaataat
     7561 aaatttcttg caaatttacc cattctactt ttatctacaa aaaattttaa aactgcaacc
     7621 ccttgtaaaa gcatagccat aaagactatg ataaatagat tcatctgtat aattccaacg
     7681 ttaatattca ataaccgcaa taaaaacgat aatagcagca atccggccat agaaatcata
     7741 aaatttcttg gaaacgaaaa ataagctatt ccttcatgtt gttttacatt tattgaaaac
     7801 cgtactgcaa atttacttgc aaccatataa ttaatatatg atgtgactac tgatgctact
     7861 atcaataccg caggaaatac atttgttaca atgtaattca tcatcggtgc cattttttca
     7921 atatcattta tatactgctc aatttgctca gttctaagtt ggtcaggaat gttgttaaat
     7981 aatcctcttg tcgtttcaaa gctttgctca tatatttccg caatcctttc gataaaatta
     8041 atgcccatta tataattcaa aagtaatatg taaagaacaa atgaaatcat ataagcagcc
     8101 gaaccgaaca aaattgtttt attagcttct tcatttctgc agacaccata tgtaagtgca
     8161 agagctaaag gtgtatataa aattaaaaat acaagtcccg tttgtaggcc caatagcatg
     8221 gaaattatta aatccgaagc tattaatgat aaaaccgaat atttaagccc cttcctttta
     8281 cccaaaataa ttgcaggggt gggatagaaa aacattaata atgtaataaa agatgacaaa
     8341 taagcaatta taaccaatat cccggttatc attgccgatt ccgtaatgtt tgatgttttt
     8401 aagtttctgt tcatctttac ctcacatatc tatatacgag agagatatca ttgtgttgtt
     8461 ttaacatttt aacctataat ctaatcaaag tcaaaaactt atttcaagct ttacgtaatt
     8521 ac
//
LOCUS       DAOPUE010000077         8420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8431_length_8420_cov_9.209564, whole genome shotgun sequence.
ACCESSION   DAOPUE010000077 DAOPUE010000000
VERSION     DAOPUE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8431_length_8420_cov_9.209564"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKH42_07325"
     CDS             complement(<1..287)
                     /locus_tag="PKH42_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HOA20280.1"
                     /translation="MNLIELLSIGVEKNASDVHLTVGIPPTYRIDGALISLVERKLTA
                     EDTLYLVKQALNETRMNKLDETGEIDFSYSIPSIGRFRVNAFKQRGSYAMVL"
     gene            complement(307..1986)
                     /locus_tag="PKH42_07330"
     CDS             complement(307..1986)
                     /locus_tag="PKH42_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOA20281.1"
                     /translation="MQKSNWKLGELLLYAGKISKEQLNEALEDQKINKKKLGEIIVDK
                     AWLKFEEIVETLELQLGFPKVDLSKYEINSNVATLIPESIVKKYKLIAIDIKDDMLLV
                     AMSDPLNFFAIDDIKLYTKMGVIPCLSSVPDVERAIERYYSSILTQKALNEFSDAFIP
                     AEDDTDSEEEMLEVATAPIVKLINSIIEQAMRGNASDIHIEPSHNDIRVRFRIDGDLK
                     EIMVLPKSNLSAITTRVKIIGRMDIAEKRIPQDGRVEMKFNGNEVDLRISTLPTVYGE
                     KIVIRILDKSNFNFTKEGLGFSKDNLDKLNKMIAQPYGMLLVTGPTGSGKTTTLYTVL
                     RELNVQTKNVITVEDPVEYKLKGINQTQVNVKAGLTFAAGLRSILRQDPDTIMIGEIR
                     DSETAEIAVRAAITGHLVLSTLHTNDSPSTIARLIDMGLEPYLVSSAVIGIISQRLVK
                     LLCPKCKEKYEANDSEKKLLGIDGNKDAMLFKPIGCNSCYNGYRGRAAVHEVMLVNEN
                     IRRLINTGANTDDIRSQALKDGMSTLLQSAADLALKGDTSFEEIMRAGYTL"
     gene            complement(2314..2697)
                     /locus_tag="PKH42_07335"
     CDS             complement(2314..2697)
                     /locus_tag="PKH42_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HOA20282.1"
                     /translation="MKSLIRLRMSTQDAHYGGNLVDGARMLGLFGDVATELLIRNDGD
                     EGLFVAYDNVEFLAPVYAGEFIEAEGEIVSKGNSSRKMVFEARKVIVPRTDISDSACD
                     VLEEPVVVCRASGTCVVPKGKQRQK"
     gene            complement(2796..4007)
                     /locus_tag="PKH42_07340"
     CDS             complement(2796..4007)
                     /locus_tag="PKH42_07340"
                     /inference="COORDINATES: protein motif:HMM:NF021493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOA20283.1"
                     /translation="MFRKLYRNETGQTIVLVALMMVMLIGFGALTIDVGAMTFQKSNL
                     QNAADSAALAGVQNMTSVSDVKKAVIDYARSNGLKVTEDGAPQSGDTITINTPYNGDS
                     KKVEVIITREVKHHLAGVLGLTQSNISARAVAEKSGFLGAAFNYAVFHGSEDDPLRFN
                     SSSIDVDGDFHSNHAIQFNSLYANIKGTLEAASLLLINSATIDIRDVCLVSSITKNSS
                     TLNVNGSTILNPAPIVEMPDFSDILKEEAQTGITYTGNKTFNSGNFSFNQPIYVTGDL
                     TLNSGNFYFDCPIYVEGKLTINSTNFIGKGIIYAKNDIKVNSASFATTAGTVSLYSGK
                     NIELNSMWADINGFMYAPNGTIIMNSMSSQVNGSVIGNEIKLNSGYLKVNYGGGNYDG
                     LPDGGSVKLIE"
     gene            complement(4047..5108)
                     /locus_tag="PKH42_07345"
     CDS             complement(4047..5108)
                     /locus_tag="PKH42_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20284.1"
                     /translation="MESDNNIVGILRDKDEADLDGVLKTTFGGYTKQSVQEYLSLIHK
                     QQQTTKETFSKNLQNLFDEKEDLRKNNETLQARLNKLSSEYDNLAGSLKDIVLDDSEY
                     SAQNVLELKNKIVSLEEQIKVTNRENYSLERKTEQLNNEIDELKSKLALSIKEYEAQK
                     EMLKSERLELKRQRDVVADISNKLQEEKNEVKYLKGTISDGKFAELNSKIGQLSTQLS
                     AQTEVNSKLNEEIALKDKTIDSLNDEVSLLKEKTNNLMKTLQEYNIQNDKLLIANEAL
                     DYQLQEEYKKAIAFINEKSNITIDRLIAQKNLSVAEAKIAALEVQLLKTNSSIEVKKE
                     INRMDTGVSDKEQKENMSF"
     gene            complement(5108..5440)
                     /locus_tag="PKH42_07350"
     CDS             complement(5108..5440)
                     /locus_tag="PKH42_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOA20285.1"
                     /translation="MKLYVNNNLLSNNITIADSFKTKLIGLLNKKSLDKNEGLLLKNC
                     TGIHCFFMKFTIEAVYLSKDMKVLYKETVKPWEMGRLVRNCTHVLELPEGMAKDIEIG
                     DYVILEGL"
     gene            complement(5440..6372)
                     /locus_tag="PKH42_07355"
     CDS             complement(5440..6372)
                     /locus_tag="PKH42_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20286.1"
                     /translation="MALYISGFFAIFIFSAIIIILSSCGLNHDKVNNRVSKLIDIESN
                     IIEDEELNKSLSERFLSPIMNSIVKSLGKFLPDTGLNNQKSENLRKSLRQAGFTLKPK
                     EYSAIRIFIIWGNALLYFVLGLVLNVSYLYLFLLPLFGAYLAFTVMRFNLMLRITKRK
                     DAMERQLPDVLDMLSVNVEAGLGFEQALLHIIEHFEGPLIDEFNITYREMSMGRSRRD
                     ALILLGERCDIDDLKSFTGSIIQANKLGISLKNVLHTQAASIRENRRSKIEEKAHKMS
                     IKILLPMVIFIFPVIFIILMGPAVVSIAEMFGGM"
     gene            complement(6380..7324)
                     /locus_tag="PKH42_07360"
     CDS             complement(6380..7324)
                     /locus_tag="PKH42_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20287.1"
                     /translation="MEILISLMAAVFIYSIFSILISSYGPGQVRNRISKYFTEYNADD
                     VHEQFIREKNEEESKRKKNRFKIASKEFSNYIASSGIRLKANEFIYFWAGLTLIPMIL
                     VLLFSGNIITGAALSIIGISIPPFIVSNATKKREDLFTKQLGDATVIMGNSIKGGFTF
                     LQSMENIAEEMQPPISTEFSKVLREIHFGVKQEDALSHMVERTKNKDLELLISAVMTS
                     AQVGSNLSEILDTISNTIRDRIRIKNEIKTLTAQGRMSGLVIGLLPIVLILAIMIMTP
                     SYFAGFFDSTIGKILISISIVLEITGFAIINKIVNIKV"
     gene            complement(7333..>8420)
                     /locus_tag="PKH42_07365"
     CDS             complement(7333..>8420)
                     /locus_tag="PKH42_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOA20288.1"
                     /translation="LLKEIYNDVMGLGPLEALLNDDDITEIMVNGPSQVYVERKGKLE
                     LSDVVFKDNTHVMNIIDRIVSSVGRRIDESSPMVDARLKDGSRFNAVIPPLSLVGPSM
                     TIRKFSRSPFTASDLVKFGSLSPKMVAFLEACVKGRMNIIVSGGTGSGKTTLLNVLSS
                     SIPNNERIVTIEDAAEIQLAQEHVVTLESRPPNLEGSGQITIRDLVRNSLRMRPDRII
                     VGEVRAGEAIDMLQAMNTGHDGSLTTAHANSPRELMSRLETMVLMSGMDLPVKAIREQ
                     IHSAIDIVVHQSRMKDGSRKVVNITEVVGMEGDTITLQDIFTYKVEGIDSNGRMKGNF
                     SSTGIRPNFLDKLTSSGIIVRDDWFVN"
BASE COUNT         2321 a         1754 c         1238 g         3107 t
ORIGIN      
        1 agaaccattg catagcttcc tctttgctta aatgcattta ctctgaatct gcctatgctt
       61 ggtatagaat atgaaaagtc aatttctcct gtctcatcta atttattcat tcttgtttca
      121 tttaatgctt gtttaactaa atataatgtg tcttcggcag ttaattttct ttccactaat
      181 gaaattagtg caccgtctat tctatatgta ggtggtattc ctacagttaa atgaacgtca
      241 gaagcatttt tttctactcc aatacttaat aattctatta agttcatatt gtcccccttg
      301 tccttcttat aatgtatatc ccgctctcat tatttcttcg aatgaagtat ctccctttaa
      361 ggctaaatca gcagctgatt gaagtaaggt actcatcccg tcttttagag cctgacttct
      421 tatatcgtct gtgtttgcac ctgtgtttat aagtcttctt atattttcat ttacaagcat
      481 tacctcgtga accgctgctc tccctctgta tccgttgtaa catgagttac agccaatcgg
      541 tttgaaaagc attgcatcct tattcccatc tatacccaat aatttctttt ctgaatcatt
      601 tgcttcgtat ttttctttgc attttggaca caaaagtttt actaatctct gagatataat
      661 tcctattacg gctgacgata ccaagtatgg ctccaatccc atatctataa gccttgctat
      721 ggttgatggg ctgtcgtttg tatgcagcgt ggacagtacc aagtgacctg taatagctgc
      781 cctgactgca atttctgccg tttcggagtc tcttatttcc cctatcatta tagtatccgg
      841 gtcctgcctg agaattgagc ggagccccgc ggcaaatgtc aatcctgcct ttacatttac
      901 ttgtgtttga tttatacctt ttaatttata ctcaacgggg tcttctactg tgattacatt
      961 tttagtttga acattcagct ctcttagtac cgtataaagt gtggtagttt taccgcttcc
     1021 cgtaggtccc gttactaaca gcataccgta aggctgtgct atcattttgt ttaatttatc
     1081 taaattgtct ttgctgaaac ctaacccctc tttggtaaag ttgaaattgc ttttgtccaa
     1141 tattcttata actatttttt ctccgtatac cgttggcagt gtggaaattc ttaaatcaac
     1201 ttcattgccg ttgaatttca tctcaactct gccgtcttgg gggattcttt tttctgctat
     1261 gtccattctt cctatgattt tcaccctggt ggttatggct gacaaatttg atttcggcaa
     1321 taccattatt tcttttaaat caccgtctat acgaaatctt actctgatat cgttgtgact
     1381 tggctcaata tgtatatccg atgcattgcc cctcatagct tgttcgatta ttgagtttat
     1441 taattttact atgggtgcag ttgcaacttc aagcatttct tcttcagaat ccgtatcatc
     1501 ttccgccgga atgaaggcat ctgaaaactc atttaatgct ttttgtgtca atatgctgct
     1561 gtagtatctt tcaatagctc tttccacgtc cggaacagat gaaagacaag gtattacacc
     1621 catcttcgta tataatttta tatcgtctat agcaaaaaag ttcaaagggt ctgacatagc
     1681 aacaagcagc atatcgtctt ttatatctat tgctattaat ttgtattttt ttacaatact
     1741 ttcaggtatc aatgtagcta cattggagtt tatttcgtat ttacttaaat caactttggg
     1801 aaatccaagc tgcagctcta atgtttctac aatttcttca aattttaacc acgccttgtc
     1861 aacgatgatt tcccctaact tcttcttatt tatcttttgg tcctccaatg cctcatttaa
     1921 ttgctctttc gaaattttac ctgcatataa taataactct ccaagtttcc agtttgattt
     1981 ttgcaattgt ttaccctcat gttttttgta ttcagctata ttacacttat agtgtattgc
     2041 agcttgattg attataattt aatataaatt ttttgtcaat ttaattattt aatctgcggt
     2101 tattgtcaat atttaatata taatgtttac ctttatgttg aatttagtat atattgttac
     2161 aattacattt ctattcttta tgaattatat ttctatttca ttttaattac attttaatta
     2221 catgaaatca tatacaggtg ccaaaactcc gtttaagctc ttgccggttt caattcaatg
     2281 ctcagtctgc ggatttttca tatattcaag ttcttatttt tgtctttgtt tcccctttgg
     2341 aactacgcat gtgccagatg ccctgcacac cactacaggc tcttctaaaa catcacatgc
     2401 cgaatcactt atgtctgtac gtggtactat tacctttctt gcttcaaata ccatttttct
     2461 tgaagaatta ccttttgaaa ctatttcacc ctctgcttca ataaattccc ctgcatatac
     2521 gggtgccaag aattccacat tgtcatatgc aacgaacaaa ccttcatctc catcatttct
     2581 gataagaagc tctgttgcca catccccgaa taatcccagc atccttgctc catcaactaa
     2641 attgccgcca taatgagcat cctgtgtact cattctaagt ctgattaatg atttcatttc
     2701 gtatttcacc ctccaaaatt tcttgctttc tcttgctgct ttgccctgca acaacttaat
     2761 attagcattt tatagagggc ttgtcaaatt tttaattatt ctatcagttt cacactgccg
     2821 ccatccggaa gaccgtcata attaccgcct ccatagttaa ctttaagata tccgctattg
     2881 agttttattt catttccgat aacacttcca ttaacctgag aagacatgct gttcattata
     2941 atagtcccgt ttggagcgta cataaatccg ttaatatctg cccacatact attaagctct
     3001 atattcttcc ctgaataaag actcactgtg ccggctgttg tcgcaaaact ggcactgttt
     3061 accttgatat cgttctttgc ataaatgata cctttaccta taaaattagt actgttaatg
     3121 gttaatttcc cctccacata aataggacaa tcaaaataga aattcccact gttaagagtt
     3181 aagtctcccg ttacatagat aggctgatta aacgagaaat ttccactgtt aaatgttttg
     3241 tttccggtgt atgttatacc tgtttgtgcc tcttctttca gtatatccga gaaatccggc
     3301 atctcaacaa tcggtgcagg attaagaatt gtacttccat taacatttag agtgcttgaa
     3361 ttctttgtga tgcttgaaac aagacataca tccctaatat ctattgtagc actgtttatc
     3421 aataataagg atgcagcttc gagtgttccc ttaatattag catacaaact gttaaattga
     3481 atcgcatgat tagagtgaaa atcgccatca acatcaatac tgctgctgtt aaatctcaac
     3541 gggtcatctt cggagccatg gaaaaccgca taattaaacg ctgcacctaa aaaaccggat
     3601 ttctcggcaa cagctcgggc agatatattt gattgcgtta atcccaaaac acctgctaaa
     3661 tgatgcttaa cttctctggt tataattact tcaacttttt ttgaatcccc attataagga
     3721 gtgtttattg taattgtatc tcctgattga ggtgctccat cctctgttac ttttaaccca
     3781 ttgcttctgg cataatctat aactgctttt ttgacatcgc ttacgcttgt catattctgt
     3841 actcctgcca atgccgccga gtctgccgca ttctgcaagt tgcttttttg aaatgtcatc
     3901 gctcctacat ctatcgtcaa tgcaccgaat cctataagca tcaccatcat taaagcaact
     3961 aaaactattg tttgcccggt ttcatttctg taaagtttcc taaacatatt attttccccc
     4021 tttattaaaa ttagcttctc tctcagttaa aatgacatat tctctttttg ctctttgtct
     4081 gaaacacctg tatccatcct atttatttct ttttttacct caattgagct gtttgtttta
     4141 agaagctgta cttccaatgc agcaattttt gcttctgcca cacttaaatt tttctgtgca
     4201 attaatctgt ctattgtaat attagatttc tcattaatga atgcaattgc ttttttgtat
     4261 tcttcctgca attgataatc taaagcttca tttgcaatca gtaatttatc attttggata
     4321 ttgtattcct gtaatgtttt cattaaattg tttgttttct ctttaagcag agaaacttca
     4381 tcattaagcg aatcaatggt tttatctttt aaagcaattt cttcgtttaa tttgctgtta
     4441 acttctgttt gtgctgacaa ctgagtgctt aattgtccaa tttttgaatt taattctgca
     4501 aacttcccgt cagaaattgt tccttttaaa tattttactt cgtttttttc ttcctgcaat
     4561 ttgttcgaaa tgtctgccac aacatctcgt tgtcttttca gttcaagtct ttcagactta
     4621 agcatttctt tctgagcttc atattccttt attgaaagtg ccagcttgga ttttaactcg
     4681 tctatttcat tgtttaattg ctcagttttt ctttccaagg aatagttttc acgatttgtt
     4741 acctttattt gttcttccaa ggatacaatt ttgtttttta attccaaaac attttgagct
     4801 gaatattcag aatcatcaag aacgatatcc tttaatgagc cggctaaatt gtcgtattcc
     4861 gaagataatt tatttagccg tgcttgaagt gtttcattgt tttttcttaa gtcttccttt
     4921 tcatcaaata aattttggag gttttttgaa aatgtttcct ttgttgtttg ctgctgcttg
     4981 tggattaatg ataagtattc ctgcactgat tgctttgtat agcccccaaa ggttgttttt
     5041 aaaacaccgt ccaaatccgc ttcatcttta tcacgcaata ttcctactat attattatcg
     5101 ctttccatta taatccctcc aaaattacat aatctccaat ttcaatatct tttgccatac
     5161 cctcgggaag ctccaaaaca tgggtgcagt ttctaacaag cctccccatt tcccatggct
     5221 tgacagtttc tttatataat actttcatat ctttagatag gtagactgct tcaatggtga
     5281 atttcatgaa aaagcaatgt atcccggtac agttttttaa aagcagccct tcatttttat
     5341 ccaagctttt cttatttaat agtccaatca gtttggtttt aaaactgtca gctattgtaa
     5401 tattatttga cagcaggtta ttattaacat atagtttcat tacatcccac caaacatttc
     5461 cgctatggac acaacagccg gtcccattaa tattataaaa ataacaggga aaataaatat
     5521 aaccatcggc aatagaattt taatggacat tttgtgtgcc ttttcttcta ttttgctgcg
     5581 cctgttttct ctgatggagg ctgcctgtgt gtgcagcaca tttttaagag atatacctaa
     5641 tttgttggct tgaattatag agccggtaaa ggattttaag tcatcaatat cacagcgctc
     5701 gcctaatagt atcagtgcat ctcttctgct tctgcccatg gacatttctc tgtaagtaat
     5761 gttaaattca tcaatcaacg gtccctcaaa atgctctata atatgcagca gtgcttgttc
     5821 aaaccctaat cctgcctcta cattaacgct tagcatatct aaaacatcgg gaagctgtct
     5881 ttccattgca tcttttcttt tggttatgcg gagcattaag ttaaaccgca ttacggtaaa
     5941 agctaaatat gctccaaata acggtaataa aaataaatat aaatagctga catttaatac
     6001 taagccaagt acaaaatata aaagtgcatt tccccatata ataaatattc ttatagcaga
     6061 atattccttt ggctttaaag tgaaccctgc ctgtcttagt gattttcgta aattttctga
     6121 tttttgatta ttaaggcctg tatcaggcag aaacttgcct agggatttaa caatagaatt
     6181 cataataggg cttaaaaacc tttctgacaa tgatttattc agctcttcgt cctcaatgat
     6241 attactctca atatcaatta atttagaaac cctgttatta accttgtcgt ggttcaaacc
     6301 gcagctgctt aatataataa taattgcact aaatatgaaa attgcaaaaa atccggaaat
     6361 ataaagagcc atatgcacct cacactttta tatttactat tttatttata attgcaaacc
     6421 ctgtaatttc taatacaata gatattgaga ttaatatttt gcctattgta gaatcaaaaa
     6481 aaccggcaaa ataacttgga gtcataatca ttattgctaa aatcagaact attggaagca
     6541 atcctataac taatccggac atgcgtcctt gggctgtcaa tgttttgatt tcatttttta
     6601 ttctaatgcg gtctctgatt gtatttgaaa tggtatcaag aatttccgat aaattgcttc
     6661 ctacttgtgc tgaagtcatt acagcggata tcaaaagctc caaatcttta ttttttgtcc
     6721 tttccaccat gtgactgagg gcatcttcct gcttgacgcc aaaatgtatt tctctaagaa
     6781 cctttgaaaa ttcagtagat attggcggct gcatttcctc tgcaatattt tccatagact
     6841 gcaaaaatgt aaaaccgcct ttaatgctgt ttcccattat tacagtggca tcacctaatt
     6901 gcttagtaaa caagtcctca cgcttttttg ttgcattact gactataaac ggcggtatgg
     6961 aaatacctat aatagataag gcagctcctg taattatatt cccgctgaaa agcaaaacca
     7021 aaatcatcgg aattaaagtt aagcctgccc aaaaatatat gaattcattt gctttcaacc
     7081 ttatccccga agaagctata taatttgaaa attccttgct tgcaatttta aatctattct
     7141 tttttctttt gctttcttct tcattttttt ccctgatgaa ttgctcatga acgtcatcgg
     7201 cattatactc cgtaaaatat tttgaaatcc tgtttctgac ctgtcccggt ccatatgagg
     7261 atatcaaaat agaaaaaatt gaataaataa atactgcagc cataagagat attaatattt
     7321 ccatatttca cctcaattta caaaccagtc atctctgacg attataccgc ttgatgtcag
     7381 cttatcaaga aagttaggcc gtatgcctgt tgatgaaaag tttcctttca ttcttccgtt
     7441 gctgtcaata ccctcaacct tgtatgtaaa tatatcctgc aatgtgatgg tatccccttc
     7501 cattcctaca acctcagtga tatttactac tttcctggag ccatccttca ttcttgactg
     7561 atgaactact atatcaatgg cagaatgtat ttgctccctt attgccttaa caggcaaatc
     7621 cattccggac atcagtacca ttgtttccaa acgagacata agctctcttg gcgaattggc
     7681 atgagctgtt gtgagtgagc cgtcatgacc tgtattcatt gcctgaagca tgtctattgc
     7741 ttcaccggca cgcacctctc cgacaattat tctatcggga cgcatacgca aagaattcct
     7801 tactaagtct ctgatagtaa tttgaccaga gccttctaaa tttggaggtc tgctttcaag
     7861 tgtaaccaca tgttcctgag ccagctgaat ttccgcagca tcttcaatag ttactattcg
     7921 ctcattattg ggaatactgc tagacaatac attcaataag gttgttttac cgctgccggt
     7981 acctcctgaa acgattatgt tcattcttcc ctttacacat gcctccaaaa acgcaaccat
     8041 tttaggagat aatgagccga attttaccaa atcacttgca gtaaacggac ttctggaaaa
     8101 tttcctgata gtcatagaag gtcccactaa tgaaaggggc ggtataacag cattaaaacg
     8161 ggagccgtct tttaatctgg catcaaccat tgggcttgat tcatcaatac gtcttcccac
     8221 tgatgataca atacgatcta taatattcat tacatgtgta ttgtctttga atacaacatc
     8281 tgataattct aattttcctt ttctctccac atacacttga gaaggaccat ttaccataat
     8341 ttctgttata tcatcatcgt tcagcaatgc ttcaagaggt cctaacccca ttacatcatt
     8401 gtaaatttct tttaataatt
//
LOCUS       DAOPUE010000078         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_8879_length_8093_cov_11.247076, whole genome shotgun sequence.
ACCESSION   DAOPUE010000078 DAOPUE010000000
VERSION     DAOPUE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8879_length_8093_cov_11.247076"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKH42_07370"
     CDS             <1..985
                     /locus_tag="PKH42_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HOA20289.1"
                     /translation="VYAGIFLYYVVATIFPIDTIIGRVYPIFGAFLIISAVGIFFGIL
                     FGGGQYLTNLSFNGMFSQHPGGLPFIPTFFITVACGILSGFHATQNTLIARTVSSEKE
                     GKTTFFNMMLVEGFIAMCWAGGAMVLFGKGTDMATGAVLMVGAVSRDFLGSIGALFAI
                     IGVIVLPITSGDTAFRGLRLMVAEQFNIDQGPAAKRVSLSIAFFIPAAVILYIAKTNP
                     AGFSILWRYFAFTNQFVATFALATISIYLYINGKNYLVSLIPGTFYMFITLSFILNAK
                     IGFNLDTLLGLPAGSYTASYILAAVLSVGYIWFVKQRATKQKDQILQNVLS"
     gene            1148..2680
                     /gene="cls"
                     /locus_tag="PKH42_07375"
     CDS             1148..2680
                     /gene="cls"
                     /locus_tag="PKH42_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOA20290.1"
                     /translation="MEKIISFFVSRATLVTLALLAQIITLALMIWRFSNYFVIFDIIF
                     MVISVLVVLYILNRKSDPTYKIAWIIPIMLFPVFGGLFYLMFGGTGLSSKMKDKMHTI
                     VDKMKESLYQHPVTLNNLRKENTIAFNQAKYIEEYSSCPVYDNSPTKFLASGEEFFKH
                     LTEELKKAQKYIFIESFIIEEGIMWNTILEILKEKAKQGLDIRVIYDDFGCVTRLPHN
                     YNKILEGYGIKCSVFNPYIPILSFRLNNRNHRKIAVIDGKTAYTGGVNLADEYINAVE
                     KFGHWRDSMIEIKGDAVWSLTVMFLSMWGYLRKIDENYRDFRPDDYDMSEECHGYVQP
                     FDDSPLDSEHVGETVYLNLINQAEKYIYIETPYLIIDNKMMVALTMAAKRGVDVRILT
                     PFIPDKWYAFAVTQSNYEDLMDVGVSIYQYTPGFNHGKTFVVDDEYAVVGTINLDFRS
                     LYLHFECGVWMYNTESVGQVKESILEALKISRQVRMEDIKKTARFKKIARTVLRIFAP
                     LM"
     gene            2697..3266
                     /locus_tag="PKH42_07380"
     CDS             2697..3266
                     /locus_tag="PKH42_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOA20291.1"
                     /translation="MKKNIKFYWKLFLSTFTLSAFTFGGGYVIVPLMRKKFVEELNWI
                     EEEEMLNIVAISQSAPGMIAVNTSIMTGYRLAGVFGAIIATFGTVLPPLIIITIIAGF
                     YTAFKENRIINALLLGMRAGVAAVIIDVIIKMARNVIKTKNNISIAIMVTALIAAVVF
                     DINAALIIILSGVFGGFYYNIVKRTGESQ"
     gene            3263..3835
                     /locus_tag="PKH42_07385"
     CDS             3263..3835
                     /locus_tag="PKH42_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOA20292.1"
                     /translation="MIYLEIFLSFFQIGLFSFGGGYAALPLIEAQVLEYHNWLTIEEF
                     ADLLTISQMTPGPIAINAATFVGTKVAGLPGAVIATIGSVTPSFIIVLTLSYFYFRYK
                     NLSSIQNILKGLRPAVVSLIASAGLSILMLTVFKTEGITLLNIKLSNINFVAIIMVVI
                     SVFILRKFKIDPIKVMLGTGVLGVLVYGLF"
     gene            3850..7326
                     /locus_tag="PKH42_07390"
     CDS             3850..7326
                     /locus_tag="PKH42_07390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOA20293.1"
                     /translation="MSNNKFTHLHLHTEYSLLDGACRIKELVKRAKELNMDSLAITDH
                     GSMYGVVEFYKQARKEGIKPILGFEAYVSPRKMTDKDPQNDKTQYHLVLLAENREGWQ
                     NIIKLCSIGFVDGYYYKPRIDHETLKEYSSGIIALSACLAGEVQTYLLEDNYDEALKT
                     ALLYKDIFGENNFFLELQDHGMEEQKAVNSSLLKLSKETGIKLVATNDVHYINKDDAY
                     FHDVLLCIQIQKTIVDEDRMKFPSDEFYLKSYEEMAELFPEEVLRNTADIAERCNVEL
                     DFNTVHLPEFKVPKGYEKSEYFKKICQEGLKERYKNITEEIQQRLDYEIGVIEQMGYV
                     DYFLIVWDFIKYARDNNIMVGPGRGSAAGSLVAYSMKITNIDPLKYNLIFERFLNPER
                     ISMPDIDVDFCYERREEVIDYVKEKYGQERVAQIITFGTMAARGSIRDVGRALNMPYG
                     EVDFIAKKIPMTLGMTISTALEVNKELKDLYDSDIKVNKLINLAMKVEGLPRHSSTHA
                     AGVLISKEEVTKYVPLSRNKDVVTTQFNMVELEELGLLKMDFLGLRTLTVIRDAIELI
                     KENHNIKIDFQNCNYDDKEVYKLFANAETLGIFQFESSGMRAFLSELKPSEFENLSAA
                     NALYRPGPMGQIPVYIKNKLNPDKVTYLHPKLEPILNVTYGCMVYQEQVMQIARDIGG
                     FTMGRSDSLRRAMGKKKMDVMLKERQNFIYGKRAENGEPEIKGAVKNGVDEKTANEIY
                     DLMVEFAKYAFPKAHSVAYAALAYETAWLKTYYPVEFMAALISSIMGSAGTVSLYIRE
                     CKRLGIEILPPDINESMGKFTVENGKIRFGLAAVKNVGVNVIQEIVKAREKKGKFTSF
                     ADFCSKVDPLVLNKRQIESLIKCGAFDFLNVRRSQLMAIYERTIDSVLAQKKRNIEGQ
                     FSLFDSMETEVDIVDELPELPEYNEKALLSMEKEMVGIYLSGHPLSEYEKILEKYATT
                     NSSEISEIMDANEGNVLYDGSRVVMGGIIIKKQNKITKNNNMMAFITLEDLYGTVEGI
                     IFPKIYDDCKDILYEDNIVLVEGTIDTSEEESPKLIINKVTELKKEYENDIKRSKLYI
                     KVKDTESYKNLKKDLFYCICKHKGEDCVIIYNEKDKASMVLSDKYRVNTEDEILFKDL
                     KKLVGEKNIAII"
     gene            7345..>8093
                     /locus_tag="PKH42_07395"
     CDS             7345..>8093
                     /locus_tag="PKH42_07395"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOA20294.1"
                     /translation="MRKLAVLTSGGDSPGMNAAIRAVVRYSIYNNISVIGIRNGFQGL
                     VEGDHTEMTLSSVADIIHRGGTILGSARSDEFMKEEGFSKALKNLKYLGIEGLVIIGG
                     NGSFKGAQKLEKAGVSTVCIPGTIDNDLPYTRYTLGFFTALTTVLESVEKIRDTSSSH
                     NRSNVIEVMGRNCGDIALYAGLAGGAEMVIIPEIEYNIDEICDKIMQGRTRGKRHSII
                     ILSEGAGDAREISELIEKKTGIITRYSILGYT"
BASE COUNT         2836 a         1261 c         1718 g         2278 t
ORIGIN      
        1 tgtttatgca ggaattttct tatactatgt tgttgcaact attttcccga tagatacaat
       61 aataggaaga gtttacccaa tatttggtgc attcctgatt atatcagcag ttggtatttt
      121 ctttggaata ttgttcggtg gaggacagta tctgacaaac ttatcattta acggaatgtt
      181 tagtcagcat cccggaggac taccctttat tcctacattc tttataacag ttgcttgcgg
      241 tatcttatca ggattccatg caactcaaaa cacattgata gcaagaaccg tttcaagtga
      301 aaaagaagga aaaacaacat tctttaatat gatgcttgtt gaaggcttca tagcaatgtg
      361 ctgggcaggc ggagcaatgg tactgtttgg taagggtaca gatatggcta ccggtgctgt
      421 attgatggta ggagctgtat caagagactt cttaggaagc ataggagctc tttttgcaat
      481 aatcggtgtt atagttcttc cgataacatc aggagacaca gcattcagag gattgagatt
      541 aatggttgcc gagcagttta atatagacca gggacctgct gctaagagag taagcttaag
      601 tatagctttc ttcatccctg cagccgtaat cctgtatatt gctaaaacaa atcctgccgg
      661 attctcaata ttgtggagat actttgcatt tacaaaccaa tttgttgcaa catttgcatt
      721 ggcaacaata tcaatttacc tctacattaa cggcaagaat tacctggtat cattaattcc
      781 gggaacattc tatatgttca ttacactaag cttcatatta aatgcaaaga tcggttttaa
      841 tttagatact ttacttggat tgccggccgg ctcatacact gcatcatata tattagcggc
      901 agttttatct gtaggatata tatggtttgt aaaacaaaga gcgacaaaac aaaaagatca
      961 aatattgcaa aacgtattat cataaacaaa aattatttta atatgtcaag gctgcctatg
     1021 gcagcctttt tgtgcagctg atcaaggaaa aatagatttg tatgattgag actgtcatta
     1081 aaactcattt acaaatactt cattttttga tacaatttac gtaatatata tagaaaaagg
     1141 gagctttatg gaaaaaatta ttagcttttt tgtaagcaga gccacattgg taacactggc
     1201 actcttggct caaataatta cactggcatt gatgatatgg agattcagca attatttcgt
     1261 aatatttgat ataatcttca tggtcatcag cgttctggtg gtattgtaca tattaaatcg
     1321 aaaaagcgac cccacttata aaatagcatg gattattcca attatgcttt ttccagtgtt
     1381 tggcggattg ttttatttga tgttcggagg gacaggatta tcaagtaaaa tgaaagacaa
     1441 aatgcatacg attgtagata aaatgaaaga atccctttat caacatcccg ttactttgaa
     1501 taatcttaga aaagaaaata ctattgcgtt caatcaagcc aagtacatag aggaatactc
     1561 gtcatgtcct gtttacgata attctccaac aaaatttttg gcatcaggtg aagagttttt
     1621 caagcatttg actgaagaac taaaaaaagc ccagaagtat atatttatag agtcttttat
     1681 aattgaagaa ggtataatgt ggaatacaat tttggaaatt ctaaaagaaa aagcaaagca
     1741 gggcttggac ataagagtta tttatgatga cttcggatgt gtgacaagac ttcctcacaa
     1801 ctataataaa atactagagg gatatggaat taaatgcagt gttttcaacc cctacatacc
     1861 gattttgtca tttcgcctca ataacagaaa ccacaggaaa atagcggtaa tagacgggaa
     1921 aaccgcttat actggaggag taaacctagc ggatgaatat attaatgccg ttgaaaaatt
     1981 cggacactgg agagacagta tgattgaaat aaaaggagat gcagtgtgga gtttaacggt
     2041 tatgttttta tccatgtggg gctatctaag gaaaatcgat gaaaactatc gggactttag
     2101 acctgatgat tatgatatga gtgaagaatg ccacggatac gtccagcctt tcgatgacag
     2161 ccccttagac agtgagcatg tcggcgaaac cgtatatttg aatttaatca accaagcgga
     2221 aaagtacata tacatagaaa caccatacct cattatcgat aacaaaatga tggtagcatt
     2281 aacaatggca gcaaaaagag gagtggatgt aagaattctt actcccttca taccggacaa
     2341 atggtatgca tttgctgtaa ctcaatctaa ttatgaggat ttgatggatg taggagtgtc
     2401 tatttaccaa tacactccgg gctttaacca cggcaagaca tttgttgtag atgatgaata
     2461 tgcggtggta ggcaccataa atttagattt cagaagcctt tatctgcatt ttgaatgcgg
     2521 agtatggatg tataacacag aaagcgtcgg gcaggttaaa gagagtatat tggaagcttt
     2581 aaaaataagc aggcaagtaa gaatggaaga tattaaaaaa acagctagat ttaaaaaaat
     2641 agcaaggact gtactcagaa tatttgcccc gctgatgtag acaaacgagg acctatatga
     2701 aaaaaaacat taaattttac tggaaattat ttttatcaac ttttacgtta agcgctttta
     2761 ctttcggagg aggatatgta atagtacctc ttatgaggaa gaaatttgtc gaagaactta
     2821 attggataga agaagaagaa atgctgaaca tagttgccat atcccagtct gcaccaggca
     2881 tgattgctgt caacacttca attatgaccg gctacaggct tgcaggagta tttggagcta
     2941 ttatagctac ttttggaaca gtactgccgc ctttaatcat aataacgata atagccggat
     3001 tttatacagc ttttaaagaa aaccgcatta taaatgccct tttattgggg atgcgtgccg
     3061 gtgttgctgc cgtaataatc gatgtaataa taaagatggc aagaaatgtt ataaaaacca
     3121 agaataatat ttccatagca attatggtta ctgccttaat tgctgccgta gtttttgaca
     3181 taaatgccgc tttaataata atacttagcg gagtttttgg aggattctat tacaacatag
     3241 ttaaaaggac cggtgaaagt caatgatata tttagaaata ttcttgagtt tttttcagat
     3301 aggattattc agcttcggag gaggttatgc tgcacttcct ttaatagaag ctcaagtttt
     3361 ggaatatcat aactggctta caatagaaga atttgcagat ttgctgacca tatcccagat
     3421 gacgccggga cctattgcaa ttaatgcagc aacatttgtg ggaactaagg ttgcggggct
     3481 ccccggagcc gtcattgcta caatagggag tgtaacccct tctttcatta tagtattaac
     3541 actgtcatat ttctatttta gatataagaa tttatcatca attcaaaata tactgaaggg
     3601 attaagaccg gcggttgtat cattaattgc ttctgcgggg ctatccattc taatgttgac
     3661 agtattcaaa acagaaggaa taaccctgtt gaatattaaa ttaagcaata tcaattttgt
     3721 tgcaataata atggtggtaa taagtgtttt tatacttaga aagttcaaaa ttgacccgat
     3781 aaaagtaatg ttagggacag gggtattagg agtattggtt tacggactgt tttgatataa
     3841 ggagaaaaaa tgtcaaataa caaatttaca catcttcatt tgcatacaga atacagcctt
     3901 cttgacggag cctgcagaat taaagagctt gtaaaaagag ccaaggaatt aaatatggat
     3961 tctctggcta taaccgacca tgggagcatg tacggagtag ttgagtttta taaacaagca
     4021 agaaaagaag gaattaaacc gatacttgga tttgaagcat atgtatctcc tcgtaaaatg
     4081 accgataagg accctcaaaa tgataaaact cagtatcatt tagtgctctt ggcagaaaac
     4141 agggagggct ggcaaaatat aatcaagctt tgttccatag gttttgtgga tggttactac
     4201 tacaagccaa gaatagacca tgaaacattg aaagaataca gcagcggaat tattgcatta
     4261 agtgcatgtc ttgcaggtga agtacagaca tatttattgg aagataatta cgatgaagca
     4321 ttaaagacag ccttgttgta caaggacata ttcggagaaa acaacttttt ccttgaattg
     4381 caggaccacg gcatggaaga gcaaaaagcc gtgaacagct ccttgttgaa gctcagcaaa
     4441 gaaacaggca taaagttggt tgcaacaaat gacgttcatt atataaacaa ggatgatgct
     4501 tactttcacg atgtgcttct ttgtattcaa atccagaaaa caatagtgga tgaagatagg
     4561 atgaaattcc cgtcggacga attttacctg aaatcctatg aagaaatggc tgaacttttc
     4621 cccgaagaag tactgagaaa taccgcagat attgcagaga gatgcaatgt agagctagat
     4681 tttaacactg tgcacctgcc tgaatttaaa gttcctaagg ggtatgagaa atcagaatat
     4741 ttcaaaaaaa tatgccaaga gggattgaag gaaagatata aaaatatcac tgaagaaatt
     4801 cagcaaagat tggactatga aataggtgta attgagcaga tgggatatgt ggattatttt
     4861 ctcatagtgt gggattttat caaatatgcc agggacaaca acattatggt gggacccgga
     4921 agaggctctg cagcaggaag cttggttgca tattccatga aaataacaaa tattgaccct
     4981 ttaaaataca acttgatttt tgaaagattt ttaaatcccg agcgtatcag tatgccggat
     5041 attgacgttg atttttgcta tgagcgccgt gaagaagtaa tagactatgt aaaagagaaa
     5101 tacggtcagg aaagagttgc ccagataata acattcggca caatggcagc aagaggctcc
     5161 atccgtgatg ttggaagagc attaaacatg ccctacggcg aagtggattt tatcgccaaa
     5221 aaaatcccca tgacattggg aatgacaata agcacagcct tggaggtaaa caaagaatta
     5281 aaggatttat atgacagcga tataaaagtc aataagctga taaatctggc catgaaagtt
     5341 gaggggcttc caagacattc ttcaacgcat gcagccggtg ttctcatatc caaggaagaa
     5401 gtaacaaagt acgttcctct ttcaagaaat aaagacgttg taacaactca attcaatatg
     5461 gttgagcttg aagagctggg actgttaaaa atggacttcc tagggttaag aactcttacg
     5521 gtaatacgtg atgccataga actaattaaa gaaaaccata atataaaaat agattttcaa
     5581 aattgcaatt atgatgacaa ggaagtttac aagctttttg caaatgctga aacgctgggt
     5641 atttttcagt ttgaaagttc aggcatgaga gcatttttaa gcgagctgaa gccttcggaa
     5701 tttgaaaacc tctcagccgc caatgctctt tacaggccgg gccccatggg gcagataccc
     5761 gtatatataa aaaataagct aaatcctgat aaggttacct atcttcatcc gaaattagaa
     5821 cccattctta acgtaaccta tggctgcatg gtataccagg aacaagttat gcaaatagca
     5881 agagatatcg gcggatttac aatgggtcgc tccgattcct taaggcgtgc catgggtaag
     5941 aaaaaaatgg acgtcatgct aaaggaaagg caaaacttca tttacgggaa aagagcggaa
     6001 aacggggagc ctgaaataaa aggtgctgtt aaaaacggtg ttgatgaaaa aacagccaat
     6061 gaaatttatg atttaatggt tgaatttgct aaatatgcat ttccaaaggc gcattccgta
     6121 gcttatgccg cccttgcata tgaaactgca tggctaaaaa cctattatcc cgttgaattt
     6181 atggcagcgc tcatatcaag cattatggga agcgccggga cagtatctct atacattaga
     6241 gagtgcaaaa gacttggaat agaaatactt ccgccggata ttaatgaaag tatgggaaaa
     6301 tttacggtgg aaaacgggaa aatacgcttt ggcttagctg ccgtaaaaaa tgtaggggta
     6361 aatgtaatac aagaaatagt taaggcaaga gaaaaaaagg gtaagtttac aagctttgct
     6421 gatttttgct cgaaggttga cccccttgta ttaaacaaaa ggcaaattga atcattgata
     6481 aaatgcggcg cttttgactt tttaaatgta agacgctcac agcttatggc aatttatgag
     6541 aggacaatcg attcagtgtt ggctcaaaag aaaagaaata tcgaaggtca gttttccctt
     6601 tttgattcaa tggaaacaga agtcgacatt gtggatgaac tgccggagct gcctgaatat
     6661 aacgaaaaag ctcttttatc catggaaaaa gaaatggttg gaatatattt aagcgggcat
     6721 cctctttctg aatacgaaaa gatattggaa aagtatgcta caacaaattc cagtgaaata
     6781 tcagaaataa tggatgctaa tgaaggaaat gttttatacg atggcagcag ggttgttatg
     6841 ggcggaataa ttataaaaaa acaaaataaa ataacgaaaa ataacaatat gatggcattt
     6901 ataacattgg aagacttgta cggaaccgtg gaaggaataa tttttccgaa aatttacgat
     6961 gactgtaagg atattctata tgaagacaat atagtattgg ttgaaggaac aatagacacc
     7021 tccgaagaag aatcaccaaa gcttattata aataaagtga ccgaactgaa aaaagagtat
     7081 gaaaatgata ttaaacgaag taaattgtat ataaaggtga aggatacaga gagctataag
     7141 aatctaaaaa aagatttatt ttattgtata tgtaaacaca aaggagagga ctgtgttatt
     7201 atctataacg aaaaagataa agcaagtatg gttttatcag ataaatatag agtaaataca
     7261 gaagatgaaa tattatttaa agacttaaaa aaactggttg gagaaaagaa tatcgcaatt
     7321 atatagttgt gagaggaggc aatgatgaga aaattagcag tattaacaag cggaggcgac
     7381 tcaccgggca tgaatgcagc cataagggca gttgtcagat actctattta taataacata
     7441 agtgtaatag gaattcgcaa cggattccag ggtttggttg agggagacca tacggaaatg
     7501 actctatcct ctgttgcaga cattattcac agaggaggga ctatccttgg aagtgctaga
     7561 agcgatgagt ttatgaagga agaaggcttt agcaaagcct taaaaaattt aaagtacttg
     7621 gggatagaag gattggtcat aataggagga aacggctctt ttaagggcgc acaaaagctt
     7681 gaaaaagcag gtgtttccac tgtgtgtatt cccgggacca tcgataatga tttaccatat
     7741 acgaggtata ccttaggatt tttcacagct ctcacaactg tattggaatc ggttgaaaaa
     7801 ataagagata catcatcatc tcacaacagg tcaaatgtta ttgaggttat gggaagaaac
     7861 tgcggcgata ttgcacttta cgcagggctt gcaggcgggg ctgaaatggt tattattcct
     7921 gaaattgaat ataatataga tgaaatatgc gataaaataa tgcagggaag aaccagaggg
     7981 aaaagacaca gcattataat tctttcggaa ggagcaggtg acgcgaggga aatatcagag
     8041 ctgatagaga aaaagacagg gataataaca agatattcca tacttggtta tac
//
LOCUS       DAOPUE010000079         8001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_9025_length_8001_cov_8.504027, whole genome shotgun sequence.
ACCESSION   DAOPUE010000079 DAOPUE010000000
VERSION     DAOPUE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9025_length_8001_cov_8.504027"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..841)
                     /locus_tag="PKH42_07400"
     CDS             complement(419..841)
                     /locus_tag="PKH42_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOA20295.1"
                     /translation="MEYKRFDNTILARIDKGEEILEKLMEICEKEHVKLANVNALGAI
                     GEFTIGLFDTSEKKYYSANYTGDYEIVSFTGSISTMDGKLYNHIHLSAGARDNKVYGG
                     HLNKAVVSATCEMFIHIVNGTVNRKFDEEVGLNLLDFN"
     gene            complement(858..1886)
                     /locus_tag="PKH42_07405"
     CDS             complement(858..1886)
                     /locus_tag="PKH42_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20296.1"
                     /translation="MLLFRIGPRHLFLRTENIKEVSNFLVTKLKGEVVGFWDGFVKAS
                     ENSTLCFITGIDHEKTYVEDAKEIVLINDSSSPILCSIISNLVSDLLQKVDMGPASIV
                     MRIPPGNADKIIEKTKEIFDGQEVDWIEGIGIGEKDDTIISFTRQELSRAVTDFLDTK
                     LLISKPVNEVKDRLRLEGLRLITHSLDDSQWYELRINIYDSYGNYQQHYERLMFILSK
                     LEIGMVLGESWTKDFAVILYSVLTYQVRLFTFHTPIEIKKILMALEYTADGKRLVDFD
                     LYYKNKKVYWYEVNKNRGKGIEKSDEAKNQRQNLYKKLKPEDIKKLEKMEKDNLDALK
                     KVLSGSKN"
     gene            complement(1897..2520)
                     /locus_tag="PKH42_07410"
     CDS             complement(1897..2520)
                     /locus_tag="PKH42_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA20297.1"
                     /translation="MKNIKDIYKGLLSLINYPHISIDFLKDLKGPLLLHISDTPSEIY
                     NNIFKIIDIIKPQYIVHTGDLADNIKLKIQKDRINSYCKGVKELVEGLEKNDSKVYYL
                     MGNHDDYEVVSQFTKKGEILEEGLLPIDNLTITVGHYYKEHSFKSDFNLYGHSFEPGH
                     YKKDGTIGLNGVLNINIIDLSNKEVFHINYPNGTNHLRCMKTKKIGL"
     assembly_gap    2666..2765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2826..3470)
                     /locus_tag="PKH42_07415"
     CDS             complement(2826..3470)
                     /locus_tag="PKH42_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOA20298.1"
                     /translation="MKENLTELVFILDRSGSMSGLETDTIGGFNSMLERQKKESGEAF
                     VTTVLFDDKYEVLHDRYDIKGVNLLTEKEYYVRGSTALLDAIGITINNIGKALSDTKE
                     EDRPGKVLFVIITDGMENSSREFSYEKVKKMVEHQKSKYAWDFIFLGANIDAIKTANS
                     FGISADMATNFVSDSVGTQLNYKIVSEVVSSFRKGKNVDKSWKDEIEKDYKKRG"
     gene            complement(3545..>4468)
                     /locus_tag="PKH42_07420"
     CDS             complement(3545..>4468)
                     /locus_tag="PKH42_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOA20299.1"
                     /translation="LKMGGGVCGAIFRAAGEKELQAECDSIGGCETGNAVITKGYKLP
                     AMHIIHTVGPVWYGGKNNEEKLLRSCYINSLNLAKEHKLESIAFPLISSGIYGYPKAE
                     AFKVAVTAIQDFLLENEMMVYLIVFDKAAVEISEKLYTSIEKYIDDKYVDEKAEIREN
                     RLQVEDAAIHYMVSAPTLIKTTKDKKSLEDIVSQLDETFSEMLLRLIDEKEMTDVETY
                     KKANIDRKLFSKIRTDKFYRPRKTTALALCIALELNLDQTKDLLARAGYALSPSSKFD
                     LIVEYFIEQGIYNIFEINEALFAFEENLLGV"
     assembly_gap    4469..4568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4569..6256)
                     /locus_tag="PKH42_07425"
     CDS             complement(<4569..6256)
                     /locus_tag="PKH42_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOA20300.1"
                     /translation="MKTCTLTINNKKITVEAGTTLLEAAKSAGYFIPTLCNHPDQEIK
                     ANCRICVVEDKSAGKFVASCSTKVYDGAEIHTNTKEVRELQRGILELILANHPNDCLN
                     CIRNESCELRKLCERFHVRNYNLNHETDIYPIDNTSGVLVRDRNKCIKCMRCVEACQE
                     TQMVGILSPSYRSVNTNISAGFGENYDETLCTFCGQCSAVCPVGAIYEYDETEKVWEA
                     LHDPKKHVIVQTAPAVRVGLGEEFGMPRGSKVTGKMVSALKMLGFDKVFDTDFAADLT
                     IMEEGYELLSRIKNGGTLPMITSCSPGWINYIEGFYPELLDHLSTCKSPQQMFGAVAK
                     TYYAKKAGIDPADIFTVSVMPCTAKKFECKRPEMNSSGYRDVDVALTTRELARLIKNA
                     GIDFTNLEDEDFDSIMGSSTGAAVIFGATGGVMEAALRTVYEVVTGKTLEKIEFDEVR
                     GLEGIKEATIDLDGTPVKVAVAHGLANANKVMEMIKNGEADYTFIEIMSCTGGCAGGG
                     GQPVGANKSIKARRIDTIYNIDKEMPIRKSHDNPEVQQLYEEFLGEPNGHLSHKL"
     gene            complement(6265..7497)
                     /locus_tag="PKH42_07430"
     CDS             complement(6265..7497)
                     /locus_tag="PKH42_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOA20301.1"
                     /translation="MGKSLSILTKNFKNINVDTIDDYLSAGGYEGLKKVLSITPAEVI
                     EVVKASGLRGRGGAAFPTGKKWEQAARVKEDSYILCNADEGEPGTFKDRYFLEYDPYR
                     IIEGMTIAAYAVGGREGYIYIREEYGRQKEIFLKAIEAAKAAGFLGENILGTDLSFDI
                     KVVSGAGAFICGETTALIQSIEGGSGRPRIKFQRTAVSGLFNRPTLANNVESYCVVTT
                     LFNNDAEEYLSHGTEKSKGTKVISLCGNVENPGVYEVPFGITLREIIYDIGGGIKDGR
                     DFKFAQIGGASGPLIPKSMLDIPYSYEDFGKEGYSLGSGAVLVADDTNSVADFMVTVQ
                     EFFVHESCGKCTPCREGNRQLLKLAHKIADKKVTVEDFLTVKRIANVMDKCSFCGLGT
                     AAPTAMVTAIQHFYDMYN"
     gene            complement(7482..7985)
                     /locus_tag="PKH42_07435"
     CDS             complement(7482..7985)
                     /locus_tag="PKH42_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOA20302.1"
                     /translation="MSKNLLDANQYEEIDKILEKHDMSKHNLVGILSDVQAMSPEHYI
                     TKEKAEYISDKLGIFLSNVYDTITFYAALSDKPKGKYVIQICNSTCCKVMKYEKIKDA
                     IEKELKVGIGEITPDGVFSFEYSSCFGACDISPAIRIGEKVYGNLDETKVIDIIKNYR
                     GGTWENL"
BASE COUNT         2181 a         1716 c         1130 g         2774 t
ORIGIN      
        1 cctataaaat tcgccacttc ggttatagtg ggcggctctt taaatctcga tactgctaat
       61 atgaaaagcc actgcttagt agtaaagttt tcgtcaaatt catcgcctaa tgtctgcagc
      121 ctgttagata aggtaaaaat tgttgcaaaa atatatgctt tctgtttaag ttcattcatt
      181 aattttcctc cagtatatgc aacatgttac ccatattgta tatggcttgc ttctctttgt
      241 caagaaaaaa agacaaagtt taaaactttg tccgagttgt tagggtatcc cattatgtca
      301 tcctgagctt aagcgaagga tctcaggttt tataagatga gattcttcga gctaaagctc
      361 tcagaatgac acgtagctta ttcatagcaa tgacaatcgt tatatacttt gtctaactct
      421 agttaaagtc aagcaggtta agtccgactt cttcgtcaaa ttttctgtta accgtaccgt
      481 ttactatatg tataaacatt tcacatgtgg cgcttacaac cgccttattc aaatgacctc
      541 cataaacttt attgtccctg gcaccggcac ttaaatggat gtggttatat agtttaccat
      601 ccattgtaga aatactcccc gtaaatgata ctatttcata atctcctgta taatttgcag
      661 aatagtattt tttttcactt gtatcaaata atccaatcgt aaattctcca atcgctccca
      721 aagcatttac atttgccagt ttaacatgtt ccttttcgca aatttccatt aacttctcaa
      781 gtatttcttc acctttatca attcttgcca atattgtatt atcaaatctt ttatattcca
      841 tttttcactc tcttccttta atttttagag ccggatagaa ctttttttaa ggcatccaag
      901 ttatccttct ccattttttc gagtttttta atatcttccg gcttaagctt tttatacaag
      961 ttttgtcttt gatttttagc ttcatctgac ttttcaatac cttttcctct attcttgtta
     1021 acctcgtacc agtaaacttt tttattttta tagtacaaat caaagtcgac taacctcttg
     1081 ccatcagctg tatattccaa tgccattaaa attttcttaa tttctattgg agtatgaaaa
     1141 gtaaacaatc tcacctggta agtcaatact gaataaagta tcacggcaaa gtccttagtc
     1201 cagctttctc ccaataccat gccgatttcc agcttagata gtataaacat aagtctttca
     1261 tagtgttgct gataattccc gtaagaatcg tatatgttaa ttctaagctc ataccactgg
     1321 ctgtcatcca agctatgagt tattaacctc agtccctcta atcgcaatct atccttaacc
     1381 tcattaacag gctttgaaat caataatttt gtatctaaaa aatctgtaac cgctctgctg
     1441 agttcctgtc ttgtaaaaga aatgatagta tcatcctttt ctccaattcc aatcccttct
     1501 atccaatcaa cttcttgacc gtcaaaaatc tccttggttt tttcaattat tttgtctgca
     1561 ttaccgggtg gtatgcgcat aactattgaa gcaggtccca tgtcaacctt ttgcagcaag
     1621 tctgaaacaa gattgctgat tattgagcat aatattggcg aagatgaatc gtttataagt
     1681 acaatttcct ttgcatcctc cacataggtt ttttcatggt caataccggt gataaaacat
     1741 aatgtactgt tctcagatgc ctttacaaat ccgtcccaaa aacctacaac ttccccctta
     1801 agctttgtaa ctaaaaagtt tgaaacttct ttaatatttt cagttcttag aaacaagtgc
     1861 cttggtccga ttctaaacag cagcataaca taccccctat aagccgattt ttttagtttt
     1921 catgcatctc aaatgattag taccattagg ataatttata tgaaatacct ctttatttga
     1981 taaatctatt atatttatat ttaatacccc gtttaaaccg attgtaccat cttttttgta
     2041 atgtcccggc tcaaaactat gaccgtaaag gttaaaatca gacttaaatg aatgctcctt
     2101 gtagtaatgc cccactgtaa ttgttaaatt atcaataggt aaaagtcctt cctctaaaat
     2161 ttcacccttc ttggtaaatt ggctgacaac ttcataatca tcatggttgc ccatcaaata
     2221 ataaaccttt gagtcattct tctccaatcc ctcgacgagt tctttaactc ccttacaata
     2281 gctgttgatt ctgtcttttt gaatttttag cttaatatta tcggccaagt caccagtatg
     2341 cacaatatat tgcggtttta taatatcgat tattttaaaa atattattat aaatttctga
     2401 aggggtatcg cttatatgaa gcaataaagg tccttttaaa tcctttaaaa aatctattga
     2461 aatatgagga taattaatta aggataaaag tcccttataa atatccttta tgttcttcat
     2521 tgcctgcaat tctccttttt aatcaaatta agcagtcccc caatatgcaa attgaagttc
     2581 tgtcatcttg tcattaatct ttattcagat tttaatcatt atatataatt tttatcacta
     2641 aagcaatatt aatttatggt gccagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnaattt atggtgccag gcaccacatc ggtgcctggc accattcaca gacaccattc
     2821 acaatttatc cccttttttt atagtctttt tctatttcat ctttccagct cttatcaaca
     2881 tttttccctt ttctgaagct gcttactact tcactgacaa ttttgtaatt caattgtgtt
     2941 cctacgctgt cgcttacaaa attagttgcc atgtctgcac tgatgccaaa agagtttgca
     3001 gtctttatag cgtcaatatt ggcaccaaga aaaataaagt cccaagcata tttagatttt
     3061 tgatgctcaa ccatcttttt tactttttca tatgaaaatt ctctgcttga attttccatg
     3121 ccgtcggtta taataacaaa cagtaccttt ccgggtctat cttcttcctt tgtatcggac
     3181 aaagctttac cgatattatt gattgtaatt ccgattgcat cgagcagcgc ggtagaacct
     3241 cgcacatagt attccttttc cgtcagtaag ttaacgcctt ttatatcata tctgtcatga
     3301 agcacttcgt atttgtcatc aaacaataca gtagtgacaa atgcttctcc gctttccttt
     3361 ttctgccttt caagcataga attaaatccg cctattgtat ccgtttcaag accggacatt
     3421 gagccgcttc tgtccaatat gaatactaat tccgttaaat tttctttcat ttcgatacct
     3481 ccaatcattt attgattgaa ggatatcact tttatgaata taagtggtcg ctttaaatgc
     3541 gacattatac tcctaacaaa ttttcttcaa atgcaaataa agcttcattt atttcaaaaa
     3601 tattataaat accctgctca ataaaatatt ctactattaa gtcaaatttg ctgctgggag
     3661 aaagagcata accggctctt gcaagcaagt ccttagtttg gtctaaattt aattccaaag
     3721 caatgcaaag agcaagagct gttgtctttc tcggcctgta aaatttatct gtccttattt
     3781 tagaaaaaag cttcctgtca atattagctt ttttataggt ttcaacatca gtcatttcct
     3841 tttcatctat taatctcaaa agcatttcag aaaaggtttc atctaactga gatacaatat
     3901 cttctaaact ttttttatcc tttgtagtct ttatcaatgt cggcgcagac accatatagt
     3961 gtatggcagc atcttcaacc tgaagtctgt tttctctgat ttcggctttt tcgtcaacat
     4021 atttatcatc aatatatttt tcaatggaag tgtacagttt ttcgctgatt tcaactgcag
     4081 ctttatcaaa gacaatcaaa taaaccatca tttcattttc caataaaaaa tcctgtattg
     4141 cagttacagc tactttaaat gcttccgcct tgggataacc gtaaattccc gaggatatta
     4201 aaggaaatgc tatggattcc aatttatgtt ccttagctaa attaagagaa tttatataac
     4261 aactcctcaa cagtttctct tcgttatttt tcccgccata ccataccgga cccacagtat
     4321 gtataatatg cattgcaggg agcttgtacc cttttgttat aaccgcatta cctgtttcac
     4381 aacctccgat tgagtcgcat tccgcttgca gctccttttc tcctgccgcc ctgaaaatag
     4441 caccgcaaac accgccgccc attttaagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnag tttatgagac aaatgaccgt ttggctctcc taaaaactct tcatacagtt
     4621 gttgtacttc aggattatca tgagattttc ttatgggcat ttctttgtca atattataaa
     4681 tagtatcaat tcttcttgcc ttaattgatt tatttgctcc caccggttga ccgccgccgc
     4741 ctgcgcatcc tcccgtacag ctcattattt cgataaatgt ataatccgct tcaccatttt
     4801 taatcatttc cataacttta ttggcatttg ccaatccatg ggcaacagca actttaacag
     4861 gtgtgccatc caaatctatg gtagcctcct ttattccttc aaggcctctt acttcgtcaa
     4921 actcgatttt ttccaatgtt tttccggtaa caacctcata caccgttctt aaagcagctt
     4981 ccattacacc acccgttgca ccgaatataa cggctgcccc ggttgatgaa cccattattg
     5041 aatcaaaatc ttcatcctcc aggttagtaa aatctattcc tgcgttttta atcaacctgg
     5101 caagttctct ggttgtaagg gcaacatcaa catccctgta tccggaagaa ttcatttcag
     5161 gtctcttgca ctcaaatttt ttggcagtgc acggcataac cgatacggta aatatatccg
     5221 cgggatctat tcctgccttt ttggcataat atgtttttgc cacggctcca aacatttgct
     5281 gcggagactt gcaggttgaa agatggtcta acagttcagg ataaaaacct tctatataat
     5341 ttatccaccc cggactgcag gatgtaatca ttggtagggt accgccgttt ttaatcctcg
     5401 acaaaagctc atatccttct tccattattg ttaagtctgc agcaaaatca gtatcaaaaa
     5461 ccttgtcaaa tcccaacatt ttaagtgccg ataccatttt accagtaact tttgagcctc
     5521 tcggcattcc aaattcttca cctaagccga ctcttaccgc tggagcagtt tgaacgataa
     5581 catgtttttt cggatcatgc agggcttccc aaaccttttc tgtttcatca tactcataaa
     5641 tagcaccgac ggggcatact gccgagcatt gtccgcagaa tgtacaaagt gtttcatcat
     5701 aattttcacc aaaacctgca ctgatatttg tattaacaga cctgtaggaa gggcttaata
     5761 ttccaaccat ttgtgtttct tggcatgctt cgacacatct catgcatttt atgcatttat
     5821 ttctatccct taccaatact cctgaagtat tatctatggg gtatatatcg gtttcgtgat
     5881 tcaaattgta gtttcttaca tggaaccttt cgcaaagctt tctaagttca caagattcat
     5941 tgcgtatgca gtttaaacaa tcattgggat gattggctaa tatcagctcc aaaatacctc
     6001 tttgcagctc tcttacttct tttgtattcg tgtgaatttc agctccgtca taaacctttg
     6061 tagaacatga agcaacaaat ttaccggcac ttttgtcctc taccacacaa attctgcagt
     6121 ttgcttttat ttcttggtca ggatgattgc atagggtcgg tataaagtaa cctgctgatt
     6181 ttgctgcttc taataaggta gttcctgcct ctacagttat tttcttatta tttatagtca
     6241 atgtacaagt tttcatgtct cctcctaatt atacatatca taaaagtgtt gtattgccgt
     6301 taccattgct gtaggtgcag cagtacctaa tccgcagaat gagcatttat ccataacgtt
     6361 agcaatgcgt ttaacggtta ggaaatcttc cactgttact tttttatccg caattttatg
     6421 tgccaatttc aaaagctgcc tgtttccctc acggcaaggt gtgcatttgc cgcagctttc
     6481 atgaacaaag aattcctgca cggtgaccat aaaatctgca acgctgtttg tatcatcggc
     6541 caccaataca gctccggagc ccagactgta accttcctta ccaaaatctt cataagagta
     6601 tggaatgtct aacatggatt ttggaataag gggacctgaa gccccgccta tttgagcaaa
     6661 tttaaaatct cttccgtctt ttattccgcc gcctatatca tatattattt ctcttaatgt
     6721 aataccgaat ggaacctcat aaactcccgg gttttcaaca tttccgcaca agcttataac
     6781 ctttgttccc ttgctttttt cggtaccgtg acttaagtat tcctctgcat cattattaaa
     6841 cagggttgta acaacgcaat atgactcaac attatttgca agcgtaggtc tgttaaataa
     6901 acctgatacg gcagtccttt gaaatttaat ccttggcctt ccgctgccgc cttcaatgga
     6961 ttgtataaga gctgttgttt cgccgcaaat gaatgctccc gcaccggaaa ccacttttat
     7021 atcaaaggat aaatcagttc ctaaaatatt ttctcccaaa aatcctgcag ccttagcagc
     7081 ttctattgcc tttaaaaata tttccttttg cctgccgtat tcctctctga tgtatatata
     7141 accttccctg cctccgactg catatgctgc aattgtcatt ccttcaatta ttctgtaagg
     7201 atcatattcc agaaaatatc tgtctttaaa tgttcccggt tctccttcat cggcattgca
     7261 taatatgtat gagtcttctt ttactcttgc tgcctgttcc catttcttgc ccgtaggaaa
     7321 agctgctccc cctctgcctc ttaatccgga agcttttaca acttctataa cctctgcagg
     7381 agttattgac aacacttttt ttaatccttc ataacccccg gctgacaagt aatcatcaat
     7441 tgtatcgaca ttaatattct taaaattctt agttaaaatg cttaaagatt ttcccatgtt
     7501 ccacctcgat agtttttaat aatatcaata acctttgtct cgtcaaggtt tccgtaaacc
     7561 ttttctccaa tcctgattgc aggtgatata tcacaagcac cgaagcaaga gctgtattca
     7621 aacgaaaaca ctccatcagg agtaatttcg cctattccaa cttttagttc tttttcaatt
     7681 gcgtctttaa ttttttcata cttcattacc ttacagcatg tagagttgca tatttgaatt
     7741 acatattttc cttttggctt gtccgataga gcagcataaa atgtaatagt atcatataca
     7801 ttgcttaaaa atataccaag cttatcactg atatattctg ctttctcctt tgttatgtaa
     7861 tgctcgggag acatagcttg tacatccgat aatatcccta cgagattatg cttgctcatg
     7921 tcatgttttt caagaatctt atcaatttct tcatactgat ttgcatctaa taagtttttt
     7981 gacatacact cctccatttt t
//
LOCUS       DAOPUE010000080         7937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_9118_length_7937_cov_18.666582, whole genome shotgun sequence.
ACCESSION   DAOPUE010000080 DAOPUE010000000
VERSION     DAOPUE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_7937_cov_18.666582"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..393)
                     /locus_tag="PKH42_07440"
     CDS             complement(67..393)
                     /locus_tag="PKH42_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOA20303.1"
                     /translation="MMNGTNKKISHDTRELLHKWVWPDNVRELENAIEYAYYAAEEDE
                     ILPGHLQLKFTKFSDKHTPTLREAEAEAVKKALNQSKNNVTQAAKILGIGRNTLYKKI
                     KEYNIE"
     gene            705..>2516
                     /locus_tag="PKH42_07445"
     CDS             705..>2516
                     /locus_tag="PKH42_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOA20304.1"
                     /translation="MYKKILILLFVCAMLSSCKKDENVLPVVETPVEYNVEDKYELTA
                     LNSDKQGIDNTTVFQLTSKVELNDSFIKNNLEILPAEDYKIEKVSGTLHNIIPLADLR
                     NDQIYQIKINDKDEDYNYSWAFQTKKKFEVEGTLPADKSSYVPSNSGIEMYFSLSGLG
                     ELDDFFEIKPHVEGKFLYKNNTAIFVPDKFEENINYTVTIKKGYGLKDSPQKLEEDYS
                     FSFNTKLQKFAQVYFDRPLTNIYENNVKVIEAYINNENLEFDINIYQYTNADFFAESI
                     NDFADTGKFFEDSVNDIEGLTFLNALKQKPNIVNKYNNPKGLFELPDLPKGYYLLEFS
                     TDDGETNYHFLQINNMLIYYALFSNQIVVTASDGADSKGIVDAAVVLNGEHMGFTDNN
                     GMLIIDRDTSVYENKAISLRVEAKGFNDFVYADSLYFYNYYYNSQYEAYKYLRYIDTD
                     RPVYLPTDTINVWGFARHKDDKSVNKVRIELVEVHTNLVFEEKTVDLTNIGTYQTEFE
                     IKNAASEYFNFQINVYDNDLRISTKYISIGKYTKPLFTISGEFDKKFVYSGESINYKV
                     NANFFDGNPLPGLELKFGGFPTNYSGSIDYGQMDSTLK"
     assembly_gap    2517..2616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2617..5147
                     /locus_tag="PKH42_07450"
     CDS             <2617..5147
                     /locus_tag="PKH42_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOA20305.1"
                     /translation="IREYSYVGSYYRFYERPYYYLEKDDDNKQNYRLNDNINLNLKYS
                     EENVESTDKDKLILLIMRDGLIDYKITDDTKLDFEFKEEYIPNVMINSIYFKNGYMYP
                     VEFINSLYYDTTERQIHFDVTTDKENYRPGEEVLLSIKTKDENNKPIKADVNISVVDE
                     AYFALFEKRVDTLNSLYGGGWSTGLNASYLSNIDLSIESGNGAERGGGGDNFTGFRDE
                     FKDTNIFKTITTDKNGNGELKFKLADNLTSWRITYQGISDKKFAGSGTKNITVSLPFF
                     VDIIMSEEYLKDDKISASLRVFGTEAKESEEVNYEIKITNKETGKELKYAQTGIIGDY
                     TNISFDKLSEGTYEISVYAAYKDFEDGIKEEFNVVDSFVYFNNTTYYKLSEDTVLSKV
                     YSNPVITLFNESRSDFYNSLESILGNYGKRIDQTVCSMLASKYINDYFKTDLTFNEDD
                     LISEINRYEIQTSGGYSLFTYSNTDAEITAKLMNLVDNEYLRLKSKTYFKNILGNPDY
                     NSKIPAALWGLSTFKEPVLLTVYDLLENNTLEIRDKIYLSLALAEFGDFNTAKKYYKE
                     FSDSFKESGDYLYFESGADENYKYSENYELTALLSVLGCKLQDYDTSDKLFKYIYNNP
                     SKFTLSNFEQLIYIMDRNIMNLDEIKDLFGEVTVTSEGKDKTYKLNLFDRENFSVPKD
                     KIQDIKFKNIKGSIACKVEALGNKDDLDKNKTEDFSINANYTLKDSTESQMSYNHSDL
                     VKVTIMPSFSDNVVEGNYEITYVVPAGFRFIELDRKWPFWVEENGQKLRLFFYYNRQT
                     VAAIPITFYIQAAQIGEYTVDYVVIKENLETRLNYIDKEVLTVQ"
     gene            5239..6132
                     /gene="amrB"
                     /locus_tag="PKH42_07455"
     CDS             5239..6132
                     /gene="amrB"
                     /locus_tag="PKH42_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOA20306.1"
                     /translation="MSRILKITVLFLLFIAVTGSIFAYNKTVNNKEPEPYTYKGGKNI
                     ECLYYNATDFINIPYYETIKVGGDILGCIVPHHLAAKDLIHEVFQNTSKDKYKTVVLI
                     GPDHESIDKGKIFTTLSNWQTPTGILETGREITEELLKCSFVKEDADKLTIEHSASSI
                     IPFINYYLKDVKVVTLVFTKQVKIEDVETLTDILYENVDLEETLFIASVDFSHYLNLQ
                     NADKMDLVSMEAIKNKDIDKIMSFTNDNLDSPISIVTMLKLMDKAGSSENTVLNHSNS
                     ELELKKKIEETTSYITYLFYR"
     gene            6282..6941
                     /locus_tag="PKH42_07460"
     CDS             6282..6941
                     /locus_tag="PKH42_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20307.1"
                     /translation="MENYLKKLIRLIFGLFIYALGLVLCIHADIGLAPWDAFGIGINK
                     VTGISYGNVSIITGIIILIALVMIFKEKIGFATILNTILIGVFADLIINSKLIPYMQN
                     FFTGVLMLITGQIVICIASYLYIGIGLGAGPRDSLMVALGKKFPNVPIGAVRGAIEGT
                     VLLIGWLLGAKVGLGTVIYVFSISFVLQTTFRLLKFDVKAVVHESVFDTINMFKEMQA
                     S"
     gene            6956..7555
                     /locus_tag="PKH42_07465"
     CDS             6956..7555
                     /locus_tag="PKH42_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOA20308.1"
                     /translation="MCGRYYIDIDNNEIKKILEEAQRNIYENYKTGEIFPTNIAPIYI
                     EDDNKIKPALAKWGFPKWDGKGVIINARAESINERQMFKRLVLSSRCIVPASYYFEWK
                     KEEHKKDKYKITNPGSNIYMAGLYNIITDKNKQLSLFDESMDIYYTIITRSANDSVSR
                     VHSRMPLVFDVEEMNEWLKGKNIDELLKADHELFGEETK"
     gene            complement(7584..7913)
                     /locus_tag="PKH42_07470"
     CDS             complement(7584..7913)
                     /locus_tag="PKH42_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOA20309.1"
                     /translation="MEIITSYEKLQELIEKNDMVLVYFGIDDCGVCKSMKPKVENMIK
                     RYPNIHAVIVKADKSPSISARFNVFTVPVILLYIEGKETIREVRIISLDNLEQKITRY
                     CQIFYVQ"
BASE COUNT         2808 a         1165 c         1410 g         2454 t
ORIGIN      
        1 ccctgatggc ggctgtatag cccccgggac cgccgcctat gattgctatc ttcatgaata
       61 cctccttcat tcaatattgt attcttttat ttttttatat aaggtatttc gtcctatacc
      121 taatattttt gcagcttgag ttacgttgtt ttttgactga ttcaatgctt tttttacagc
      181 ttcagcttca gcttctctta gtgttggtgt atgtttatct gaaaatttag tgaatttcag
      241 ctgtaaatgc ccaggcagga tttcgtcttc ttctgcagca tagtaagcat attctattgc
      301 attttccaat tctcttacat tgtccggcca aacccattta tgaagcagct ccctggtatc
      361 atgtgatatt tttttattgg ttccattcat catattgtac tgatttaata cgtagtcaga
      421 aataatatcc agatcatcct gcctgtctct taaagtacag ctttaataat acaattatat
      481 cataagaatt tcaaatcaga agcggcattg tttaaatctt ttgaactttt ttaatcattg
      541 tatcgtcaaa tattaaatgg tataattgac ataaggcatc gaatcgcaat aatgaatacg
      601 aaaacttaca attcggcaaa ttgaataatc ttatgttttg tgaattccta aatctgttgg
      661 aaacggcata agctttgatt attactaaag aaaggcagga aattatgtat aaaaagatat
      721 tgatactttt atttgtttgt gccatgcttt cttcctgtaa gaaggatgaa aatgtattac
      781 ctgtagtaga aactccggtt gaatacaatg ttgaggataa gtatgaactt actgccctaa
      841 actcggataa acagggaatt gacaatacta ctgtttttca attgacttca aaagtagaat
      901 taaacgatag ctttattaaa aataatttag agatacttcc ggctgaagat tataaaattg
      961 aaaaagtatc aggaactcta cataatatta ttccccttgc agaccttcga aacgaccaaa
     1021 tctaccaaat caagataaat gataaagatg aagactacaa ttattcctgg gcttttcaaa
     1081 caaagaaaaa gtttgaagta gaaggtacgc tgcctgcaga taaaagcagc tatgtaccgt
     1141 caaattcagg aattgaaatg tatttttcct tgagcggctt aggagaattg gatgattttt
     1201 ttgaaattaa gcctcatgtt gaaggaaaat ttctgtataa aaacaataca gctatttttg
     1261 tacccgataa gtttgaggaa aacatcaatt atacggttac tataaaaaaa ggatacggtc
     1321 tgaaagatag cccgcaaaaa ttggaggaag attattcctt tagtttcaat acaaaattgc
     1381 aaaaattcgc tcaagtatat tttgaccggc ccttgacaaa tatttacgaa aataatgtga
     1441 aagtaattga ggcatatata aacaatgaaa atcttgaatt tgacattaat atttaccaat
     1501 atacaaatgc agactttttt gcagaaagta tcaatgattt tgcagacaca ggaaaatttt
     1561 tcgaagattc tgtaaacgat attgaagggt taaccttttt aaatgcttta aaacaaaagc
     1621 ctaacattgt gaataaatac aataatccaa aagggttgtt tgagctgccg gaccttccga
     1681 agggatatta tttactggaa ttctcaacag atgacggcga aacaaattat cacttcctgc
     1741 agattaacaa tatgcttatt tactatgctt tgttttcaaa tcaaatagtg gttactgcat
     1801 cggatggtgc agacagcaag gggattgtag atgcggcagt tgtattgaac ggggagcata
     1861 tgggctttac agacaataat ggaatgctga ttattgatag ggatacgtct gtttacgaaa
     1921 ataaggcaat ttccctaaga gttgaagcaa aaggtttcaa cgattttgtg tatgcagaca
     1981 gcctttactt ttacaactat tattacaaca gtcaatatga agcttacaaa tatttaagat
     2041 atatcgacac ggacagacct gtatatcttc ctacagatac aattaatgta tggggatttg
     2101 caaggcataa ggatgataaa tctgttaata aggttagaat tgaattagtt gaagtccaca
     2161 caaatttagt gtttgaggaa aaaactgtag accttacaaa tataggcacc tatcaaacag
     2221 agtttgaaat aaaaaatgct gcatcagaat actttaactt tcaaataaat gtatacgaca
     2281 atgatttaag aatatctaca aaatatattt caatcggcaa atatacgaaa cctcttttta
     2341 caataagtgg cgaatttgac aaaaaatttg tttacagcgg tgaaagtatt aattataagg
     2401 ttaatgccaa tttctttgac ggcaatcccc tgcccggatt agagctaaaa ttcggcggct
     2461 ttccaaccaa ctacagcgga agcatagact acggacaaat ggattcaact cttaagnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttat aagagaatac tcctatgtag
     2641 gaagttatta ccgcttttat gaaaggcctt attattattt agaaaaggat gatgataata
     2701 aacaaaatta caggctaaat gacaatataa atttaaatct taaatacagc gaagaaaatg
     2761 ttgaaagcac agataaggat aaacttatac tcttaataat gagagatggt ctcattgatt
     2821 ataaaattac tgacgataca aaacttgact ttgaatttaa agaagaatat atacctaatg
     2881 ttatgattaa ctctatatat tttaaaaacg ggtatatgta tccggtggaa tttataaact
     2941 cattgtacta tgataccaca gaaagacaga ttcactttga tgtaactaca gacaaggaaa
     3001 attacagacc cggcgaagaa gttctattaa gtattaaaac aaaggatgaa aacaataagc
     3061 cgattaaggc agatgttaat atttcagttg tagatgaggc atattttgcc ctctttgaaa
     3121 aaagagttga tactttaaat tcattgtatg gcggtggttg gagtacaggc cttaatgcat
     3181 cttatctgtc aaatattgat ttgagcatag aatcgggaaa cggtgccgaa agaggcggcg
     3241 gtggagataa ttttacaggt ttcagagatg aattcaaaga tacaaatata tttaaaacaa
     3301 taacaaccga taagaacggc aatggagaat taaaatttaa attagctgat aacttaacat
     3361 cctggagaat aacgtatcaa ggaatttcag ataaaaagtt tgcaggaagc ggcacaaaaa
     3421 atattactgt aagtttgccc tttttcgtag atataataat gagtgaagaa taccttaaag
     3481 atgataaaat atcagcttcc ttaagggtat ttggaacgga agcaaaagaa tctgaagaag
     3541 taaattatga aattaaaatt acaaacaaag aaacaggtaa agagcttaaa tacgcccaaa
     3601 caggtataat aggagattac acaaatattt catttgataa attaagtgaa ggcacctatg
     3661 aaatttccgt ttatgctgca tataaggatt ttgaagatgg tataaaggaa gaattcaatg
     3721 tggttgattc attcgtttat tttaacaata ccacttatta taaactgtca gaagatacag
     3781 tcttaagtaa agtgtattca aatcctgtaa ttacattgtt taatgaaagc cgctctgact
     3841 tttacaacag tttggaaagt attttaggca actacggtaa aagaattgac cagacagtat
     3901 gctcgatgct ggcatcaaag tatatcaatg attattttaa aacagatttg actttcaacg
     3961 aggatgattt gattagtgaa ataaacagat atgaaatcca aacaagcggc ggatacagcc
     4021 tcttcacata ttcaaatact gatgcagaga taaccgccaa attgatgaac ttggtagata
     4081 atgaatactt aagacttaaa tccaaaacat actttaaaaa tatattggga aatcccgatt
     4141 acaattcaaa aatcccggca gctctttggg gattaagcac ctttaaggag ccggtattat
     4201 tgacggttta tgatttattg gaaaacaaca cattggaaat cagggataaa atttatctat
     4261 ctttagcttt agcggagttt ggagacttta atacagctaa gaaatattac aaagaatttt
     4321 cagacagctt taaagaatca ggagattatt tgtattttga aagcggagcc gatgaaaatt
     4381 ataagtattc tgaaaactat gaattaacag cactcttgtc cgttttagga tgcaaacttc
     4441 aagattatga tacaagcgac aaattattta aatatattta taataatcct tctaagttca
     4501 ctctttcaaa ttttgagcag cttatatata ttatggacag gaatattatg aacttggacg
     4561 aaataaaaga cttgttcggc gaagtgactg ttacatcgga aggtaaagat aaaacttata
     4621 aattgaattt atttgacaga gaaaactttt ctgttccaaa ggataaaatt caagacatta
     4681 agtttaaaaa tattaaaggc agtatagcct gcaaggttga agcattgggg aacaaagatg
     4741 atttagataa gaataagacc gaggattttt caatcaatgc aaattatacg cttaaagatt
     4801 ctacagaaag tcaaatgtca tacaatcatt ctgacctggt taaagtaacc ataatgccat
     4861 cattcagcga caatgttgta gaaggtaatt atgaaataac atatgttgtt ccggcaggtt
     4921 tcagatttat tgagcttgac aggaagtggc cattttgggt tgaagaaaac ggtcagaaac
     4981 taagactttt cttctactat aacaggcaga ctgtcgctgc aattcctatt acattttata
     5041 ttcaagctgc acaaatagga gaatatacgg tggattatgt ggttataaaa gaaaatctgg
     5101 aaaccaggtt aaattatatt gacaaagaag ttttaacggt acagtaataa ctaaccaata
     5161 tattgggcaa aacccttgtc atttttggcc gaatgtaaat aatctcacct tttaacaaag
     5221 caaagtgagg tgtaagtatt gagcagaata ctaaaaatca cagttttatt tctgttgttt
     5281 atagcagtca caggcagtat atttgcttat aacaagacag taaataataa agaaccggag
     5341 ccgtatactt ataagggcgg aaaaaatatt gaatgcctct attataatgc aacagacttt
     5401 atcaacattc cttactacga aacaattaaa gttggtggtg atatactggg ctgtatagta
     5461 ccccatcatt tggctgcaaa ggacttaatt catgaggttt ttcaaaatac gagcaaggat
     5521 aaatataaaa ccgtagtatt gattggtccc gaccacgaaa gcatagataa aggcaaaata
     5581 tttacaacct taagcaattg gcagacaccc acaggcatat tagaaacagg cagagaaata
     5641 acagaggaat tattgaaatg cagctttgta aaagaagatg ctgataaatt aacaattgag
     5701 cattctgcct cttcaattat tccatttata aattattatt tgaaagatgt taaagtagta
     5761 accttggttt ttacaaaaca ggtgaaaata gaagatgtgg aaacattgac agatatattg
     5821 tatgagaacg tagatttgga ggaaacatta ttcatagctt cagtcgactt ttcacactat
     5881 ttaaatttac agaatgccga taaaatggat ttggtaagca tggaagcaat aaaaaataaa
     5941 gatatcgata aaattatgtc ttttaccaat gataatttag attcacccat atccatagtt
     6001 acaatgctta aactcatgga taaagcgggc tcttcagaaa ataccgtttt aaatcacagc
     6061 aattcagagc ttgaattaaa aaagaaaata gaagaaacta caagctatat aacctatttg
     6121 ttttatcggt aggcatcctt ttaggaaagg gcgttaatgt caatgcatgt taccggtcgc
     6181 tcatggaaaa ataagagccc tattttaata ctttttttta ttgttcttgt tatttttcat
     6241 ggtatcatat aaaattagct tattgaaagc aggaggattt aatggagaat tacttaaaaa
     6301 aacttattcg attaatattc gggcttttta tatatgccct gggtttggtt ttatgtattc
     6361 atgctgacat aggacttgct ccctgggatg ctttcggtat cggcattaat aaggttaccg
     6421 gaatatctta cggcaatgta tcaattatta caggtattat aattttaata gcactggtta
     6481 tgatatttaa agaaaaaata ggttttgcaa caatattaaa cacaatactg ataggagtct
     6541 ttgctgattt aattataaat tcaaagctga ttccctatat gcagaatttt tttaccgggg
     6601 ttttaatgct cataacaggg cagattgtaa tatgcatagc atcctatttg tatataggaa
     6661 taggtcttgg ggcaggacca agggattcct tgatggttgc tttaggaaag aaattcccga
     6721 atgtaccgat tggagcagtc aggggggcaa tagagggtac tgttttgtta atcggctggc
     6781 ttttgggagc caaagtggga ttaggaactg ttatttatgt attcagtata agttttgtac
     6841 ttcagacaac atttaggctg cttaaatttg atgtaaaagc agttgttcat gaaagcgtat
     6901 ttgacacaat aaatatgttt aaagaaatgc aggcttcata aacagtgagg tagatatgtg
     6961 cggaagatat tatattgata tagataataa tgaaattaaa aaaatattgg aagaagccca
     7021 aagaaacatt tatgagaatt ataaaaccgg tgaaattttc cccacaaata ttgctcctat
     7081 ttatatagag gatgataata aaatcaagcc ggctttggct aagtggggat tccccaagtg
     7141 ggacggcaaa ggtgtaataa ttaatgcaag agctgaatca ataaatgaaa gacagatgtt
     7201 taaaagattg gttttatcca gcagatgcat tgtgcctgct tcctattatt ttgaatggaa
     7261 aaaggaggaa cataagaagg acaaatataa aataactaac ccgggctcca atatttacat
     7321 ggctggatta tacaatataa ttacggataa aaacaagcag ttaagcttgt ttgatgaaag
     7381 catggatatt tactatacta ttataacccg cagtgccaat gattctgttt cccgcgtaca
     7441 cagcagaatg ccgctggttt ttgatgttga agaaatgaat gaatggctga aagggaaaaa
     7501 catcgatgaa ttattgaaag ctgaccatga gcttttcggc gaagaaacca aataactttg
     7561 ataattctat tcaatatcta catctactga acataaaata tctggcaata ccttgtgatt
     7621 ttctgttcta aattatccag gctgattatt cttacttccc tgattgtttc ttttccctca
     7681 atatataata atattacagg aactgtaaac acattaaatc ttgcagatat actcggactt
     7741 ttatccgctt taacaattac agcgtgaata ttaggatatc ttttaatcat attttccact
     7801 ttaggcttca tggacttaca tacgccgcaa tcatcaatcc caaaataaac caataccatg
     7861 tcattttttt caataagttc ctgcagcttt tcataagatg ttataatttc cattgtagtc
     7921 tccatttatg tatttat
//
LOCUS       DAOPUE010000081         7758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_9393_length_7758_cov_9.860184, whole genome shotgun sequence.
ACCESSION   DAOPUE010000081 DAOPUE010000000
VERSION     DAOPUE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9393_length_7758_cov_9.860184"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1410
                     /locus_tag="PKH42_07475"
     CDS             169..1410
                     /locus_tag="PKH42_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA20310.1"
                     /translation="MAFNMVAEHAIWPKVNDAIFGLAAKAKEAIDKYGKENVIDSTLG
                     ALVDDNGELICLNTVYKELKSLPNSAIAAYAQVAGQPDFLEVVQKVCFGKYKPNAYIR
                     AVATPGGTGSVRHGIYNYTNPGDSILVGDWHWAPYVTISEEYGRTVTNHELFNSKNEF
                     NIESFKEKFEELLNKQKRLVAVINTPANNPTGYSLSDEEWDEVLNIAKENAKDPENRI
                     IIMVDCAYIDYAGIGDERRKFFAKFSGLPKNILIIVAFSMSKGYTMYGMRSGAAIGIS
                     SDEDIAEEFYYSCMHSNRANWSNGTRAAMEIMTRIANDPEKTKLYEAEVNETKVMLRR
                     RADAFVEGAKEVGLEILPYRDGFFVSIPCKDAKKLSDELIKENVFAIPLKKGLRFAVC
                     AVSEEKCRIAPAIIKKALDRI"
     gene            complement(1487..2737)
                     /locus_tag="PKH42_07480"
     CDS             complement(1487..2737)
                     /locus_tag="PKH42_07480"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOA20311.1"
                     /translation="MKKEKVVLAYSGGLDTSIIIPWLKENYNDLDIIACCVNVGQNDD
                     MEFVKNKALSSGASKVYIENAVNEFVEEYVYKGIKANAAYEGKYLLGTAYARPLICKK
                     LVEVAHKEGAKYIAHGCTGKGNDQVRIETGIAALDPTIEIIAPWRIWDIKSREDAIDY
                     AEAKNIDLNGITKERIYSRDQNILHVSHEGGMLENPGIEPDFEELLLMTNTVEKAPDL
                     AEYVEIEFEKGIPVKLNNKKLNGEDMLKSLNKIGGTHGIGILDLLENRLVGMKSRGVY
                     ETPGGTILLEAHKYLESFVLEKEEAHFKEIVAQKYAELVYNAMWYSGFKQALDAFIDS
                     TQEKVSGKVKLKLYKGTIKFAGMTSENALYNESISSFATGDLYDQKDATGFIHLFSLP
                     YKIQAYNKMNKNNKASDVVYSFKE"
     misc_binding    complement(2867..3076)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3263..3335)
                     /locus_tag="PKH42_07485"
     tRNA            complement(3263..3335)
                     /locus_tag="PKH42_07485"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3300..3302),aa:Val,seq:tac)
     gene            3411..3695
                     /locus_tag="PKH42_07490"
     CDS             3411..3695
                     /locus_tag="PKH42_07490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOA20312.1"
                     /translation="MIKVVAKGYFLEGKAEEAIKLYEELVEKTRKEEGCISYSLFRDI
                     EDDSILTMIEEWESKDALDKHFKTEHFTKLVPMIGKLRKSSEVNIYKLVI"
     gene            3877..4080
                     /locus_tag="PKH42_07495"
     CDS             3877..4080
                     /locus_tag="PKH42_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOA20313.1"
                     /translation="MKILIRRDDKNYIPMVPYMPENPKPGYGYVPYQINPRYFNNLTE
                     AFVQGTIFPDLVTPYSEFFQRGV"
     gene            4083..4358
                     /locus_tag="PKH42_07500"
     CDS             4083..4358
                     /locus_tag="PKH42_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOA20314.1"
                     /translation="MADYNRTNQRYNQQQEMLLEVQKLQFAALDTALYLDTHPNDPVA
                     LYRHKMYTDRLKEVKDAYEMQFGPMTIFNHEVGDYWRYINSPWPWEM"
     gene            4389..4961
                     /locus_tag="PKH42_07505"
     CDS             4389..4961
                     /locus_tag="PKH42_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOA20315.1"
                     /translation="MFMYEKKLQYPVRVTNKDVRMAKAVLNQYGGPDGELSASMNYLT
                     QRFSMPLNETKAILTDIGTEELGHMEMVATLFKKLIKGATKEELIAAGMGGYFADHET
                     CPFLVDAEGEHWDSKFTQAKGDPIADLYNDMAAEQKARATYENLILLTDDPLVKDTLR
                     WLREREIVHFQRFGEGLRLVEEYTNSKRYY"
     gene            complement(5080..5448)
                     /locus_tag="PKH42_07510"
     CDS             complement(5080..5448)
                     /locus_tag="PKH42_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20316.1"
                     /translation="MSPGEIIYSIGLLITLIVIYLMVLVITMNRNIKKVVQIFEEKNA
                     LSAKTAISYTDLNIQKQGIIERAIKKRDNRIHALKFLLDGGVVIITGDERYYLNKKKM
                     TEFKKRLNFIGRMMVPDIDN"
     gene            complement(5640..7586)
                     /locus_tag="PKH42_07515"
     CDS             complement(5640..7586)
                     /locus_tag="PKH42_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOA20317.1"
                     /translation="MAEYDRESAYRRLIGFPGKLVFCLAVAWSVFQLYTGLFGTFPST
                     LQRAPHLAAALTLVYLLYPAKRGKSVEFTKHIPWYDYILAAASVFVGSYHIIFYDALL
                     LRAGSFTQLDVIVSVIAILLLLEAARRVAGLVIVSVGSVFLLYAFFGNLLPRTSFLFH
                     APFTFKRIVCTEWLGSEGILGTPIYVSSTFIFLFLVFATFLKKSGVGDWMTNLATAAV
                     GGQVGGPAKTAVIASALQGTVSGSSVGNVVATGSITIPLMKDTGYEPNFAGAVEAAAS
                     TGGQIMPPIMGAAAFIMTEYVGVTYGTVAISAAIPAILYFSGIFLNVHYEALKKGLLG
                     LPKEKRPDGKKLVKEGWYMILPVILIFGLLIGGSSAMKAAMYGIIGCIIIWIINIIKE
                     EKRFDIVKFVKMFIDSLDESARSAITVAITCGTAGIIVGVVTMTGLGLKMADGLVELA
                     GGSLLLTMIFTMFSSIILGMGVPTTANYIIQATISAPALVALDVPPIAAHLFVFYFGI
                     VADITPPVALAAFAGAGISGGNPMKTGFTATRLGIAAYIGPYMLVYNPILVLVNTGNY
                     STPIFILMVIKSAITAIIGMGGLATGFTRYFKTNCKIWESIVLIVAGLLLVDPGTVTD
                     ITGGAIMVAIYLIQKRRVAKAAIS"
BASE COUNT         2573 a         1472 c         1328 g         2385 t
ORIGIN      
        1 atggatcaat acatattaac ttttatataa tacattcctt gtcgtatctt gtcaacaact
       61 tatttaaata catcttttag caattgccgc tgaatttttc agaatttgta tcgtattgac
      121 ttatattttt ttaaatgtta gaattgaaga aaaaaatgag gtgatgtaat ggcatttaat
      181 atggttgcag aacacgcaat ttggccaaaa gtcaatgatg caatctttgg gcttgcagct
      241 aaagcaaaag aagctataga caaatatgga aaagaaaacg tgattgattc aacccttgga
      301 gcattagttg acgataatgg agagcttatt tgcttaaata cagtatataa agaattaaaa
      361 tctttaccga attcagcaat tgcagcatat gctcaggtgg caggtcagcc tgattttttg
      421 gaagtagttc aaaaagtttg cttcggcaaa tacaaaccta atgcatatat cagggctgta
      481 gcaactcccg ggggaacagg ctccgtaaga catggaatat ataattatac aaatcccgga
      541 gattcaattt tggtaggaga ctggcattgg gctccatatg taactatttc cgaagaatac
      601 ggcaggactg ttaccaatca tgaattattc aacagcaaga atgaatttaa tattgaatca
      661 ttcaaggaaa aatttgaaga attgctgaat aaacaaaaaa ggctcgttgc agtgataaac
      721 acacctgcca acaatcctac gggatacagt ctttccgatg aagaatggga tgaagtttta
      781 aatattgcta aagaaaatgc aaaagaccct gaaaatagaa ttataataat ggttgactgc
      841 gcatacatag attatgcagg aatcggagat gagagaagaa aattcttcgc aaaattttcc
      901 gggcttccta aaaatatttt aattattgtt gcattcagta tgtcaaaggg atatacaatg
      961 tatggcatgc gctcaggtgc agcaatcgga atatcttctg atgaagatat agctgaagaa
     1021 ttctattatt catgcatgca ttcaaacagg gcaaactggt ctaacggtac aagagctgca
     1081 atggaaataa tgactcgtat agccaatgac cctgaaaaaa caaagttata tgaagcggaa
     1141 gtcaatgaaa caaaggtaat gctgagaagg cgagccgatg cttttgttga aggagcaaag
     1201 gaagtgggat tggaaatttt gccttacaga gacggattct ttgtaagcat accttgcaag
     1261 gatgcaaaaa aattgtccga tgagttaatt aaagaaaatg tatttgctat accgttaaaa
     1321 aagggacttc gttttgcagt atgtgccgtt tccgaagaaa aatgcaggat agcacctgca
     1381 ataatcaaaa aagctttgga tagaatttaa tgttattaaa atattaggga cacaatatac
     1441 aatattgcgt cccttttatt tgtagatgct ctttattaat cttacattat tctttaaaac
     1501 tgtaaacaac atctgatgcc ttgttgtttt tgttcatttt gttataagcc tgaattttgt
     1561 atggcagact gaataaatgt ataaatcccg ttgcatcctt ttgatcatac aaatcaccgg
     1621 tagcaaatga tgatattgat tcattataca atgcattttc agaagtcatt cctgcaaact
     1681 taattgttcc tttatacaat ttaagtttta cttttcccga taccttttct tgggttgaat
     1741 ctataaatgc atcaagtgct tgcttaaaac cgctgtacca cattgcattg tacaccaatt
     1801 ccgcatactt ttgagcaaca atttccttaa aatgtgcttc ttctttttcc aatacaaaac
     1861 tttcaaggta tttatgggct tccaatagta tggttccgcc gggggtttcg tatactcctc
     1921 ttgatttcat gccaaccagc ctgttttcca ataaatccaa tatgccgatt ccatgggtgc
     1981 cgcctatttt atttaatgat ttaagcatat cttccccatt taatttcttg ttgtttaatt
     2041 ttacaggtat gcctttttca aattcaattt ccacatattc agccaaatca ggggcctttt
     2101 caacagtgtt tgtcattaac agcaattctt caaaatcagg ctcaataccc ggattttcaa
     2161 gcatccctcc ttcatgactt acatgaagaa tattttggtc tcgtgagtat attctttctt
     2221 ttgttattcc gtttaaatct atattttttg cttcggcata atcaattgca tcttcccttg
     2281 attttatatc ccatatacgc catggtgcaa taatttcaat tgtagggtcc aatgctgcta
     2341 ttccggtttc aatacgtacc tggtcatttc cttttcctgt acacccgtgg gcaatatact
     2401 ttgctccttc tttatgagca acttcaacaa gtttcttgca aatcaacggt cttgcataag
     2461 ctgtccctaa aagatatttc ccttcatatg ccgcatttgc cttaattcct ttataaacat
     2521 attcttcaac aaactcattt actgcatttt cgatatatac cttgcttgca ccggaagata
     2581 atgctttatt cttaacaaat tccatatcat cgttctgacc tacatttacg cagcaagcaa
     2641 taatatctaa atcattgtaa ttctctttca gccaagggat tattattgat gtgtccaatc
     2701 ctcctgaata tgctaatacc actttttctt ttttcatttt ctgctcctcc aaatttaaat
     2761 aaaactaatt ataattgcaa tgtatgcttt cttcaaagac tattttcact ttagagctgt
     2821 gtcccaatac cttcatcaac gtgtcagggc aaaaaaaaac tgcctataaa gaggcagtgt
     2881 ttacaccgcg gtaccactct tttataaagc agctgcttcg ctctttcatg ataacggtct
     2941 ttaaccgtat tctcatactg ctacttctga gaatcttttc atggactctt ttcaattaaa
     3001 ctacactacc gaactcacac tctgctccgg ctcgctgaag ctttatttaa tctactcttc
     3061 caatcataaa attatacaat taataatttt tctaaaatta tatgttgctt ttcttatttt
     3121 gtcaacatat ttttttattt tttttctaaa attattatat ttattcttta accggaatat
     3181 tcgttaattt tagatttact tagatccgat ttttatgata atatatagta taattaaaaa
     3241 tacggcacat gccgtatatt aatgggagct atagggctcg aacctatgac cccctgcttg
     3301 taaggcagat gctctcccag ctgagctaag ctcccgtata ataaaaatac cacaacttca
     3361 taaggttgtc aaaacatttt taatatttta taaaaaaata aggaggacgt atgattaaag
     3421 tagtagctaa aggttacttt ttagaaggca aagcagaaga agcaataaaa ctatacgaag
     3481 agttagttga aaaaaccaga aaagaagaag gatgcatttc ctacagttta ttcagggata
     3541 ttgaagatga ttcaatacta acaatgatag aggaatggga gagcaaagat gccttggata
     3601 aacattttaa aacagaacat ttcacgaaac tggttccaat gataggaaaa ctaagaaaaa
     3661 gcagtgaagt aaatatttat aaattagtaa tttaagaata ccttcttata caagatgatt
     3721 caaccgaatc atcttgtatt tttttataac aaatagggac taaaaatgag aatagttcat
     3781 taacaacatt tgaaaattgc gtcacttttt gtctatttat gaataatatg aaataactat
     3841 ttttttaacg aaatagcaaa tattcatagg aaaggaatga aaattttgat tagaagagat
     3901 gataaaaatt atataccgat ggtgccatat atgcctgaaa acccaaaacc gggatacgga
     3961 tatgttcctt atcaaataaa tcctcgttat tttaacaatt taactgaggc ttttgttcag
     4021 ggtacgatat ttccggattt ggttactcca tattcagaat tttttcaaag aggggtgtaa
     4081 ttatggcaga ttataacaga acaaatcaaa gatataatca acagcaggaa atgttgttgg
     4141 aagtacaaaa acttcaattt gcagcattag atactgcact ttatttagat actcacccga
     4201 atgaccccgt tgccctgtat agacataaaa tgtacaccga caggttaaaa gaagttaagg
     4261 atgcatatga aatgcaattt ggaccaatga ctatatttaa ccatgaagtt ggtgactatt
     4321 ggcgatatat aaacagcccg tggccttggg aaatgtaagt agattatata gaaaggaatg
     4381 ataactctat gtttatgtat gaaaaaaaat tgcagtatcc tgtaagagtt actaataaag
     4441 acgttcgaat ggctaaagct gttttaaatc aatacggcgg tcctgacggt gaattaagcg
     4501 ctagtatgaa ttatcttact caaagattca gcatgcctct aaatgaaaca aaagcaatac
     4561 taactgacat cggcaccgaa gaactcggcc atatggaaat ggttgctacc ttatttaaaa
     4621 agttaataaa aggtgccact aaggaagaat taattgcagc cggcatggga gggtactttg
     4681 ctgaccatga aacatgtccc ttcttagttg atgcggaagg agaacactgg gattcaaaat
     4741 ttacacaggc gaaaggagat cctatagcag acttatacaa cgatatggct gctgagcaaa
     4801 aagcaagagc aacctacgaa aacttaatat tattaaccga tgacccctta gtaaaagata
     4861 ctttaagatg gctgagagaa cgtgaaatag tccacttcca aagatttgga gaaggattaa
     4921 ggctagtaga agaatatact aacagcaaga gatattatta gttatatttt taaattatat
     4981 aatcacctat gaggatcttg ttctcatagg tgatttttgc aaatataatg ttttattact
     5041 gcatatattc aactgaagat tatttttaaa gggtattcgt caattgtcta tatcaggaac
     5101 catcatacgt ccgataaaat tcaaacgttt tttaaattca gtcattttct ttttatttag
     5161 atagtacctt tcgtcacctg ttataattac aactcctcca tccaacagaa acttcagggc
     5221 atgaatacga ttgtcccgtt tttttatggc acgttcaata ataccttgtt tctgaatatt
     5281 aaggtctgtg taacttatag ctgtttttgc agataaggca tttttctctt cgaatatttg
     5341 aactaccttt ttaatatttc tattcatggt gattactaat accataaggt aaataacaat
     5401 aagtgtgatt aatagaccta ttgaataaat tatttctcct ggcgacatta gagcagcctc
     5461 cgcttgttag tattatattt ttttcagttt aacacagcaa acactccctg tcaaaccttc
     5521 tgtttcagta tagtgtttcc attcgtaaag ataaaaacag gagattgcat tacacaacct
     5581 ccttcgagat tcgagacaag atgaaaaatt gaatttgtac atcaattata tatcaaatac
     5641 tacgaaattg cagcctttgc gacacgtctt ttctgtatta agtaaatcgc aaccattata
     5701 gctccacctg taatatcagt aactgtaccg gggtcgacaa gcagtaagcc tgcaacaatt
     5761 aaaacaatac tctcccaaat cttgcagttt gttttaaagt atcgggtaaa tccggtagca
     5821 agaccgccca tgcctataat agcagttata gcagatttta ttaccatcag gatgaagata
     5881 ggagttgaat aatttccggt gtttacaagt accaaaatag ggttataaac caacatatac
     5941 ggtccaatat aagctgctat gccaagtcga gttgcagtaa aacctgtctt catcggattt
     6001 ccaccggata taccggcacc ggcgaaagca gctaaagcaa cgggaggagt tatatcagca
     6061 actataccaa aatagaatac aaacaagtgg gcagcaattg gtggaacatc taatgctaca
     6121 agtgccggag cagaaatcgt tgcttgaatg atatagtttg cagtagtagg aacgcccata
     6181 ccaagtataa tcgagctgaa cattgtaaaa atcattgtga gaagcaaact gcctccagcc
     6241 aactcaacca gaccatccgc cattttaaga cctagaccgg tcatggtaac aacacctact
     6301 ataatacctg cagtaccgca ggttatagct acggtaatag cactacgggc actctcatca
     6361 agagaatcta tgaacatttt cacaaacttg acaatatcaa atcttttttc ttctttaata
     6421 atattaataa tccatataat tatgcatcct atgataccat acatagctgc tttcatcgct
     6481 gaacttcccc caataagcaa cccgaagata agaatgacag gcagaatcat ataccaacct
     6541 tctttaacca acttcttgcc atcagggcgc ttctccttag gaagaccaag taaacctttt
     6601 ttaagagctt catagtgaac gttcaaaaat ataccggaga aatacaatat tgccggtata
     6661 gcagcagata tggcaactgt accatatgtt acacctacgt attctgtcat aataaacgca
     6721 gcagcaccca taattggcgg cataatctgc ccgcctgtcg aagcagctgc ttcaaccgct
     6781 ccggcaaaat ttggctcata ccccgtatct ttcatcaaag ggattgttat agagccggtt
     6841 gcaacaacgt ttccaacgga agaaccacta acagttccct gcagagcact tgctattacg
     6901 gcagttttcg caggaccgcc tacttgaccg ccaacagctg ctgtagcaag gttggtcatc
     6961 caatcaccta caccgctctt cttcaagaag gttgcaaaca ccaaaaataa gaaaataaaa
     7021 gttgatgata catatatcgg tgtccccaga ataccctcgc ttcccagcca ttccgtacag
     7081 actatgcgtt taaaagtaaa cggggcatgg aagagaaatg aagttctagg cagtaggttg
     7141 ccaaaaaatg catatagcag aaacacactt cctacagata caattaccag gcctgcaacc
     7201 cggcgagccg cttcaagcag aagcaaaatt gcaataacac ttacaataac gtctaactgc
     7261 gtgaatgaac ctgcacgcaa gagtagtgca tcataaaaaa taatatgata tgagccaaca
     7321 aatacactgg ctgccgccaa aatataatca taccatggaa tatgcttcgt aaattctaca
     7381 gactttcccc tctttgcagg ataaagcaga taaaccaagg ttaatgctgc ggctaaatgc
     7441 ggtgcccgct gcagcgtaga tgggaatgtt ccaaacaaac cggtataaag ttgaaacaca
     7501 gaccatgcca cagccaaaca aaatactaac ttaccgggga accctatcaa tcgacggtat
     7561 gcagattccc tgtcatattc cgccatcaat tcgtcagcat caatgtcaac cttgtcacct
     7621 gaataaatta cttcttcttc atcgttatct tttttttctt cgtcaacttt atcagtgttt
     7681 tctttgtaaa cgatatcagt gttttcatca acagtatcga tgtgctcttc gtcaacagag
     7741 ggaataaatt tttcatta
//
LOCUS       DAOPUE010000082         7734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_9439_length_7734_cov_7.774320, whole genome shotgun sequence.
ACCESSION   DAOPUE010000082 DAOPUE010000000
VERSION     DAOPUE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9439_length_7734_cov_7.774320"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PKH42_07520"
     CDS             <1..380
                     /locus_tag="PKH42_07520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA20318.1"
                     /translation="MDLDNFKDVNDTYGHIYGDKAMSIVGEVITKSIRNTDYIGRFGG
                     DEFLLICPGAGIKEAFEVAERIRKALESHLFILSDEVSVKLTVSIGVHEYSEEDNSFT
                     DMLKKADKCLYKAKETRNKVCAD"
     gene            511..1389
                     /locus_tag="PKH42_07525"
     CDS             511..1389
                     /locus_tag="PKH42_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOA20319.1"
                     /translation="MKKSRILSIIAVALMLMIVFAGCTQETTEADFDFDKDITVVARD
                     AASGTRGAFHEIMDIIVKENNTEVDKLVVGALEFDGTDKVVTAVEGDKYGIGYISLGS
                     VSERIKALAVDGAEPSVENVKSGAYSVSRPFLLVTKGTENELIKDFLKFTESVEGQAI
                     TNEMNFIGVADSPEEYTASGMSGTIKVAGSTSVAPLMEKLQEAYNELNPDVTFETQAQ
                     GSSQGIKAAIDGSYDIGMASRELKDEEAEELNRYVLAIDGIAVIVNNENPISDLAADD
                     ITNIYIGEITKWNAVK"
     gene            1389..2267
                     /gene="pstC"
                     /locus_tag="PKH42_07530"
     CDS             1389..2267
                     /gene="pstC"
                     /locus_tag="PKH42_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOA20320.1"
                     /translation="MQTRQSNFNEIIMQAIFFLSALISVIAVLAISLFLFMRGIPAIS
                     EIGFMDFIFNEKWGPTNTPASYGILSMIVGSIYLTGGAIVIGVPIGLMCAIFLAEFCP
                     DNVYKIVKPAVNLLAGIPSIIYGFFGMQVIVPFVRESFGGNGYSILSASIILGIMILP
                     TIISISESSIRAVPESYAEGSYGIGATKEETVFKVVVPAAKSGILTSIILGIGRAVGE
                     TMAVVMVAGNAVMPIDKIEILKPIRTLTTNIVTEMSYASGLHESSLIATGVVLFVFII
                     ILNVSLSVIKSKNQRN"
     gene            2279..3142
                     /gene="pstA"
                     /locus_tag="PKH42_07535"
     CDS             2279..3142
                     /gene="pstA"
                     /locus_tag="PKH42_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOA20321.1"
                     /translation="MDRAIDNLKQRKAKAFLFKSSVWFCGILTVGILIYILFFILLKG
                     VPKINRSFLFGEYNSETLSAFSSMVTTVQMIVISLLISVPIGVFCAIYLAEYAKRGNK
                     AVKVIRLAIDTLAGIPSIVYGLFGMIFFVQILAFRASVLSGIGTISIMILPVIISSSE
                     EAIKSVPDTYREGSLGLGASRLRTVFRIVLPTAMPGILAGIILSIGRIVGETAALIFT
                     SGTNVLKTPSLDLMASQRTLAIHMYMLASEGLPYSRDMAFATGSILILVVFVVNYATT
                     KLAGRIGRIHD"
     gene            3135..3887
                     /gene="pstB"
                     /locus_tag="PKH42_07540"
     CDS             3135..3887
                     /gene="pstB"
                     /locus_tag="PKH42_07540"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOA20322.1"
                     /translation="MTDKIKIENVDLFYGDFQALKNVNMNIKENEITAFIGPSGCGKS
                     TLLKSLNRMNDLIEGVKITGRMLLDDVDIYKDMDATVLRRRVGMVFQKPNPFPMSIYE
                     NVAYGPKTHGVKGKAKLDEIVEESLRSAAMWDEVKDRLKKSALGLSGGQQQRLCIARA
                     FSVNPEVILLDEPTSALDPISTMKIEDLLVEIKKNYTIIIVTHNMQQATRISDKTAFF
                     LVGEIIEFDETERLFSIPKNKKTEDYITGRFG"
     gene            3900..4547
                     /gene="phoU"
                     /locus_tag="PKH42_07545"
     CDS             3900..4547
                     /gene="phoU"
                     /locus_tag="PKH42_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOA20323.1"
                     /translation="MRNRFDQELDKLNLELIKMGGMIENAIEHSAKALINQDLSLVKK
                     VNELEIEIDDMERTIEARCLKLLLQQQPVASDLRIISTALKMITDMERIGDNAEDIAE
                     ITKNLVNQKFIKEIVHIPQMAEETTSMVKRSIDAFVNKDKELAKEVCKSDDTVDNLFD
                     IIKEELIEKIQENKSNGEQAIDLLMIAKYFERIGDHAENIAEWVIFSITGEHKMP"
     gene            4746..5435
                     /locus_tag="PKH42_07550"
     CDS             4746..5435
                     /locus_tag="PKH42_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007555779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA20324.1"
                     /translation="MRLIYCVEDEAGIRELITLALSTADFEVKEFEDAAAFYAEMKNK
                     LPDLILLDLMLPKIDGMTVLRELKANSEFKAIPVIILTAKSLELDKVKGLESGADDFI
                     TKPFGILELLARVKAVLRRCEKKAEEKLLRYNNLVLDYNKRTLNYIDKTVALTYKEFE
                     LLHYLMLNKGIVLSRDKILQEIWGFDYEGETRTVDMHIKTIRQKLEAAGCTNYIATVR
                     SAGYKFEEQED"
     gene            5435..7177
                     /locus_tag="PKH42_07555"
     CDS             5435..7177
                     /locus_tag="PKH42_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOA20325.1"
                     /translation="MRRRIFITILIISVIASSIVSILTTYEYYSFYVEEAKQQLKTIV
                     NLSSEPHKWDSKASFSSSINSILKSVDYSIRFTIISEDGSVIHDNWKDEIEENHIDRP
                     EVQAAFEKGYGEDLRYSTTVSTDMYYYAVKLNDYKVLRLSREISSINNVFAGLIPMLF
                     VLFIFVLIIVYFAASITAKKILKPVNVMTKSLDNMLEEDSNFNIKIYEEFEPLSNTIK
                     DQKNKINEYISELKNERDNISFITGNMKEGFILLNKNKYILSINKSGKIMTGNEKFKL
                     NESRNILELTRNPEIIEKINAALNENKHLVHDVETVNNYYRYYFSPVIEHNKNFVKGL
                     LILIEDITVQKKSEIMRSEFTANVSHELKTPLTTIIGFAEMIKEGFIKDNENIQKYSG
                     LIYKEGMRLISLIEDLMRLSKIQEHNEGYEEELINVKNICEEVVNLLKSKAEQHSVKL
                     TLNADDVVMKAKSNYINELLYNLIDNGIKYNKDGGSVDVKIFDEGGFANIVVGDTGVG
                     ISPEHKDRIFERFYRIDKSRSKETGGTGLGLSIVKHITELYDGTIEIESSINNGTKIT
                     IKFPKMQPNRRGSI"
     gene            7261..>7734
                     /locus_tag="PKH42_07560"
     CDS             7261..>7734
                     /locus_tag="PKH42_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOA20326.1"
                     /translation="MNKILRINLRTNEITYEDAPRKYLETGGRGLIAKIMLDLVNPQC
                     DPLGRENKLIIANGLLTGTNVSSASRIAIGAKSPLTGGIKESNGGGITAMWLASLGLK
                     AIVIEDIPEEDEWYYIRISKNGCKLEPAGEYKGMGTFDFCEKMIDKYPDCAVTCIG"
BASE COUNT         2790 a         1117 c         1657 g         2170 t
ORIGIN      
        1 tgatggattt ggataatttc aaagatgtaa atgataccta cggccacatt tatggtgaca
       61 aagccatgag tattgtggga gaagtcatta caaagagcat aagaaatacc gattatatag
      121 gaagattcgg cggtgatgag tttttgctca tatgcccggg ggcaggtata aaggaagcat
      181 ttgaagttgc tgaaaggata agaaaagccc ttgaaagcca tctgttcata ttaagtgacg
      241 aagtaagcgt aaaacttaca gtgagcatcg gggtgcatga atacagtgaa gaggacaatt
      301 cttttaccga tatgctaaaa aaggcagaca aatgtttata taaggcaaaa gagacaagaa
      361 ataaagtttg tgctgattaa ttgaagtgaa atcagggagt atgtaaagtg taaaaacttt
      421 acatactttt tacatataga tggttttatt tttactgtga ggaggtatcc ttatctcaag
      481 aatagaaaat aaaaggagat aaaacagaaa atgaaaaaaa gtagaatatt atcaataatt
      541 gcagtggcat taatgctaat gattgttttt gcaggatgta cacaggaaac aacggaagcg
      601 gattttgatt ttgataaaga tataactgta gtagcaagag atgctgcatc gggtacaaga
      661 ggtgccttcc atgaaataat ggatataatt gttaaggaaa acaacacaga agtggataaa
      721 ttggttgttg gagcattgga gtttgacgga acggataaag tagtaactgc tgttgaagga
      781 gacaaatatg gtatcggtta catttcatta ggctctgtaa gtgaaagaat taaagctctt
      841 gcagttgacg gtgcagaacc ttctgttgaa aatgtaaaaa gcggtgctta ttcagtatca
      901 agaccatttt tattggttac aaaaggaaca gaaaatgaac tgataaaaga tttcttaaag
      961 tttacagaat cagtagaggg acaggcaatt acaaatgaaa tgaattttat tggggttgct
     1021 gattcgccgg aagaatatac agcatcaggt atgtcaggta caataaaagt ggcaggttca
     1081 acatcagtag caccattgat ggaaaaactg caggaagcat ataatgagct taatcctgac
     1141 gttacatttg aaactcaggc acaaggttca tcacaaggaa taaaagctgc aattgacggt
     1201 tcttatgata taggaatggc ttcaagagaa ttgaaggatg aagaagcaga agaattaaac
     1261 agatacgtac tggcaattga tggtattgca gtaatcgtaa ataacgagaa tccaataagt
     1321 gatttggcag cagatgatat aacaaatatt tatataggcg aaatcacgaa gtggaatgct
     1381 gttaaataat gcagaccagg cagtcgaatt tcaatgaaat aataatgcag gcgatcttct
     1441 ttctgtctgc attaatttcc gttattgcag tccttgccat cagcctcttt ctatttatga
     1501 gaggtattcc tgcaatttcg gaaataggtt ttatggattt tatatttaat gaaaaatggg
     1561 ggcctaccaa tactcctgct tcttacggga tattgtcgat gattgttgga agcatttatt
     1621 tgacaggggg agcaattgtt ataggcgtgc ccatcggctt aatgtgtgcc atatttttgg
     1681 ctgaattttg tcctgacaat gtttataaaa ttgtgaagcc cgcagtgaat ctgctggcag
     1741 gcataccttc aataatttac ggtttctttg gaatgcaggt aattgtgcct tttgtgaggg
     1801 aaagttttgg gggtaacgga tacagcatat tatcagcatc aataatctta ggaattatga
     1861 tactcccaac aattatttca ataagcgaat cctctataag agccgtgcct gaatcttatg
     1921 ctgaaggttc ctacggcata ggagcaacaa aggaagaaac agtatttaaa gtagtggttc
     1981 cggcagctaa atcagggatt ttaacttcaa ttatactggg tataggaaga gcagtaggtg
     2041 aaactatggc tgtagttatg gtggcaggta atgctgttat gcctattgat aaaatagaaa
     2101 ttctaaaacc aataaggaca ctaactacca atattgttac tgaaatgagc tatgcatctg
     2161 gacttcatga atcatccctt atagctaccg gagtagtttt atttgttttt ataataatct
     2221 taaatgtttc tttaagtgtt ataaaatcta agaatcaaag aaattaaaga ggtgtaaaat
     2281 ggatagagct atagataatt taaaacaaag aaaagcaaaa gcttttctgt tcaaaagttc
     2341 tgtttggttt tgtggaatat tgacagtggg aattttaata tatatactat tctttatatt
     2401 attgaaaggt gttccgaaaa ttaacaggtc gtttttgttc ggagaataca attcagaaac
     2461 actttctgcc ttttcatcta tggttacaac tgttcaaatg attgtaattt cacttttaat
     2521 atctgttccg atcggtgtgt tttgtgcaat ttatcttgca gaatacgcaa aaagaggaaa
     2581 caaagcagta aaggttataa ggcttgcaat agataccttg gcgggaatac cttcaattgt
     2641 gtacggtttg tttggtatga tatttttcgt gcagattctt gcatttaggg cttcggtttt
     2701 gtcagggatt ggcacaatat caattatgat attgcctgtg attataagct catcggaaga
     2761 agccataaag tcagttccgg acacttatcg cgaaggcagt ttaggattag gtgcttcaag
     2821 gcttcgaaca gtgtttcgca tagtacttcc aacagcaatg cccggcatac ttgcaggaat
     2881 catactttca ataggacgta ttgtgggaga aactgcagcg ctgatattta caagcggtac
     2941 caatgtttta aaaacccctt cactagattt gatggcatca caaagaaccc ttgcaattca
     3001 catgtacatg cttgccagtg aagggctgcc ttattcaaga gatatggcat ttgcaacggg
     3061 atcaattttg atcttggtag tgtttgtagt gaactatgct acaactaagt tagcaggtag
     3121 aatagggagg atacatgact gataaaataa aaatagaaaa tgttgattta ttttacggag
     3181 attttcaagc tcttaaaaat gtaaatatga atataaaaga aaatgaaata actgccttta
     3241 ttggaccttc aggatgcgga aaatccacat tgcttaagtc attgaacagg atgaatgatt
     3301 taatcgaagg tgttaaaatt accggaagga tgcttcttga tgatgtggat atatacaagg
     3361 atatggatgc aacagtgtta agaagaagag tcgggatggt ttttcaaaag cccaatcctt
     3421 tccccatgag catatacgag aatgtagcat atggtccgaa gacacatgga gttaaaggca
     3481 aagcaaaatt agacgaaatt gtggaagaga gtcttcgttc tgcagcaatg tgggatgagg
     3541 tcaaagatag acttaaaaag agcgctttag gattatccgg aggtcaacag cagagactgt
     3601 gcatagcgag agcattttcc gttaacccgg aggttatatt gctcgatgaa ccaacttcag
     3661 cccttgaccc catatctacc atgaaaattg aagatttgct ggttgaaatc aaaaaaaatt
     3721 acactattat aattgttacc cataacatgc agcaagccac aagaatctct gacaaaacag
     3781 cttttttcct tgttggtgaa attattgagt ttgacgaaac agaaaggctg ttttctatac
     3841 ctaaaaacaa aaaaaccgaa gattatataa caggaagatt cggatagaag gaggatataa
     3901 tgagaaacag atttgatcag gagcttgaca agttaaatct tgaacttatt aagatgggag
     3961 gcatgattga aaatgcaatt gagcacagtg ccaaagcact tataaaccaa gacctgtcat
     4021 tggttaaaaa agttaatgaa ttagaaattg aaattgatga tatggaaaga acaatcgagg
     4081 cacgctgttt gaaactgtta ttgcagcagc agccagtagc gtctgacctt agaataataa
     4141 gcacagcact taaaatgatt accgatatgg aaagaatcgg agacaatgcg gaagatatag
     4201 cagaaattac aaaaaattta gtcaatcaga aatttattaa ggaaattgtt catattcctc
     4261 aaatggcaga agaaactacc agtatggtaa aaagaagtat tgacgcattt gttaacaagg
     4321 ataaggaatt ggcaaaagaa gtatgcaagt ctgatgatac agttgataat ttatttgata
     4381 ttattaaaga agaattaata gaaaaaatcc aggaaaataa aagtaacggt gagcaagcta
     4441 ttgacttgtt gatgattgct aaatattttg aaagaatagg ggaccacgcg gaaaatatag
     4501 ctgaatgggt tatattttca attaccggag aacacaagat gccctaaccc catttatcag
     4561 aggtatgggg acacacctct actataggga tagtctttgg gggaagagga atgtccccga
     4621 ataaggtttt tcagaaaaat ggttggtatg tcattcgcaa caaattccca atttatgcga
     4681 cgtaaggagc aaataaattg gttaatgaga ctgcgagacg ccctaactca aaaggaggtt
     4741 tataaatgag actcatatac tgcgttgaag atgaagcagg cataagggaa ttaattacac
     4801 ttgctcttag tactgcggat tttgaggtaa aggaatttga agatgccgct gcattttatg
     4861 ctgaaatgaa aaacaagctt cctgacttaa tactgcttga tttgatgctg cctaaaattg
     4921 acgggatgac agttttgaga gaacttaaag ccaattctga attcaaggcg atacctgtta
     4981 taattttaac tgctaagtct ctggaactgg ataaggtaaa gggattggaa tcgggagcag
     5041 atgatttcat taccaaaccc ttcggaatat tggaactttt ggctagagtt aaggctgtat
     5101 tgaggcgttg tgaaaagaaa gctgaagaaa aactactgcg atacaataat ctggtattgg
     5161 attacaacaa aagaactctg aattacattg ataagacggt agcactaaca tataaagaat
     5221 ttgagctgct tcattacttg atgctcaaca agggaatagt gctgtcaaga gataaaattc
     5281 ttcaggaaat atggggtttt gattatgaag gagaaacaag gactgttgat atgcatatta
     5341 aaaccataag gcagaaactt gaggcagcgg gctgcacaaa ctatattgcc acggtgagaa
     5401 gtgccggata taaatttgag gagcaagaag actgatgaga agaaggattt ttattacaat
     5461 attaattata tctgttattg cttcatcaat agtaagtata cttacaacat atgaatatta
     5521 cagcttttat gtagaagaag caaaacaaca gttgaaaacc atagttaatc tttcgtctga
     5581 gcctcataag tgggactcaa aagcttcctt cagttcatcc ataaacagca tattaaaatc
     5641 cgttgattac agcatacgat ttacaattat tagtgaagat ggaagtgtaa ttcatgataa
     5701 ctggaaggat gaaattgaag aaaaccacat agacagacca gaagtacaag cagcatttga
     5761 aaagggttat ggggaagact taaggtattc aaccacagta tccactgata tgtattatta
     5821 cgctgtcaag ttaaatgact ataaggtact taggttatcc agagaaataa gcagcataaa
     5881 caacgtattt gcaggattga ttcccatgct ttttgtgttg tttatttttg ttttaataat
     5941 tgtgtatttt gctgcatcaa taacagcaaa aaaaatttta aaacctgtca atgtgatgac
     6001 caagtcatta gataatatgc tggaggaaga ctccaatttc aatataaaaa tatacgagga
     6061 attcgaacct ctttcaaata ccataaaaga ccagaaaaac aaaataaatg aatatataag
     6121 tgaacttaag aatgaaaggg acaatatatc ttttataaca ggcaatatga aggaaggatt
     6181 tatacttctt aataagaaca aatacattct ttctataaac aaaagcggaa aaattatgac
     6241 gggtaatgaa aaattcaaat taaacgaaag cagaaacatt ttggaattga ctcgaaatcc
     6301 ggaaattatt gaaaagataa atgctgcttt aaatgaaaac aagcatcttg tacatgatgt
     6361 tgagactgta aataattact acagatatta cttcagcccc gttatagaac acaacaaaaa
     6421 ttttgtaaaa ggactgttga tattaattga agatattaca gtacaaaaga aatcagaaat
     6481 catgagaagc gagtttacag caaatgtgtc ccatgagtta aaaacacctc tcacaaccat
     6541 cattggattt gcggagatga taaaagaagg ctttataaaa gataatgaaa acattcaaaa
     6601 atacagcggg ctcatatata aggaaggcat gcgtcttata tctttaattg aagatttaat
     6661 gaggctttct aaaattcaag aacataacga agggtacgaa gaagagctaa tcaacgttaa
     6721 aaatatttgt gaggaagtag taaatctgct taaatcaaag gcagaacaac acagtgttaa
     6781 actgacgctg aatgctgatg atgttgtgat gaaggcaaaa agtaattata ttaatgagct
     6841 tttatataat ttaatagata atggaattaa atataacaaa gacggcggaa gcgtagatgt
     6901 aaagattttt gatgagggag gatttgcaaa tatagttgta ggcgacacag gggtgggcat
     6961 atctcccgaa cacaaagaca gaatatttga aagattttac cgaatcgata aaagccgctc
     7021 aaaggaaaca ggaggaacag gtcttggtct ttccatagta aaacatataa cagagcttta
     7081 cgacggtacg atagaaattg aaagctcaat aaataacgga acaaaaatta ccataaaatt
     7141 cccaaagatg caacctaaca ggaggggttc tatttgagca tcctcaaaat gatttcatta
     7201 tgattaaact tattactttg agggtacact aaaagggaga tttacaaaag ggagagtata
     7261 atgaataaaa ttctgagaat taacttaaga acaaatgaaa taacatatga agatgctcct
     7321 cgcaagtatt tagaaacagg gggcagagga ttaatagcaa aaataatgct tgatttagta
     7381 aatccacaat gtgacccgtt aggaagggaa aataaactca ttattgcaaa cggactgttg
     7441 accggaacca atgtgtccag tgcaagcagg attgccatcg gggcaaaaag ccccctgaca
     7501 ggcggcataa aggaaagcaa cggaggggga attactgcca tgtggcttgc ttcccttggc
     7561 ctgaaagcta tagtaattga agatattccg gaagaagacg agtggtatta tataagaata
     7621 agcaagaatg gatgcaagct ggagcctgca ggagagtaca aaggtatggg cacctttgac
     7681 ttttgtgaaa aaatgattga taaatatccc gattgtgcag ttacatgcat aggc
//
LOCUS       DAOPUE010000083         7636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_9608_length_7636_cov_9.971508, whole genome shotgun sequence.
ACCESSION   DAOPUE010000083 DAOPUE010000000
VERSION     DAOPUE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_7636_cov_9.971508"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKH42_07565"
     CDS             complement(<1..340)
                     /locus_tag="PKH42_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HOA20327.1"
                     /translation="MCGRYYIDIDNEEFKKILEEAQRNIYENFKGGEIFPTNIAPIYI
                     EDDNKIKPLLAKWGFPKWDGKGVIINARAETLNEKLMFKKLSSSNRCIVPASFYYEWK
                     QEEKGKHKYKI"
     gene            complement(352..1017)
                     /locus_tag="PKH42_07570"
     CDS             complement(352..1017)
                     /locus_tag="PKH42_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20328.1"
                     /translation="MQIYLKKFIRLIFGLYIYALGLVLCIYANIGLAPWDAFAIGISK
                     VTGISYGNVSILIGITIVIALFFLLKEKIGFGTILNAILIGFFSDLIIDSKIIPYMQN
                     FFTGVLMLVTGQILACIASYLYIGVGLGAGPRDSLMIGLGKKFSNIPIGIVRGSIEAT
                     VLFIGWILGAKVGLGTVIYVFSIGFLLQTTFKLLKFDVKSVVHESVFDTINIFKKIHS
                     ENI"
     gene            complement(1135..2019)
                     /gene="amrB"
                     /locus_tag="PKH42_07575"
     CDS             complement(1135..2019)
                     /gene="amrB"
                     /locus_tag="PKH42_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOA20329.1"
                     /translation="MNKFFKVFTVFLLFITIAGSILAYNTAKNRHEKYFFDGRKNIEC
                     LYYDPSDFINIPEYETSQDIGTIHGCIVPHHLVAKDLIHEVFQKVQKNKYKTVVLIGP
                     DHESTNKGKIFTTLSNWQTPRGVLETDGNLTKELLKYDFVTEDDDKITMEHSTSSIIP
                     FINYYLNDIKIVTLVLTKQVKLEDVEILTNELYKNINLHDTLFIASVDFSHYLNLEDA
                     QKKDLISMEAIKNKDINKLMSFTNDNLDSPISVVTLLKMMDKLGAAGDTVLNHSNSEL
                     ILKKKIEETTSYITYLFY"
     gene            complement(2052..>4417)
                     /locus_tag="PKH42_07580"
     CDS             complement(2052..>4417)
                     /locus_tag="PKH42_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOA20330.1"
                     /translation="DKLILLIMKNGLIDYKITDNTNLSFEFEEEYIPNVMLNSIYYKN
                     GYMYPVEFTGSLLYDTTEKQIHFDVTTDKEEYRPGEEVVLSIKTKDENNKPITADVNI
                     SVVDEAYFALFEKRVDTLNSLYARVSSGLVATYLSNIDLSMDNGSGAERGGGGEDYTN
                     FRDEFKDTNVFKTITTDKNGNGELKFKLADNLTSWRITYQGISDKLFAGSGTKNITAS
                     LPFFVDVIMSKEYLKDDKISASLRVFGAEAKESEEVNYEIKIINKETGKELKYKETGI
                     IGDYTNISFDKLDEGSYEIYIYASYKDFEDGIMEEFNVLDSFVYFNNTTYYKLTEDTV
                     LDSVYSNPVITLYNESRSDFYKSLENILANSGKRIDQTVCSMLAAKYINDYFKTDLYF
                     NEDELINEVNKYEIDTWGGYSLFTYSAADVEITAKLINLIDNEYIGYKSKTYFKNILQ
                     NPDYNEEIPAALWGLSTYKEPVLLTIYDLLENNTLEIRDKIYLSLALAELGDLNTAKK
                     YYKEFSNSFKESGNYLYFESGADENSSSENYELTALLSLLGCKLKDYETSDKLFKYIY
                     NNPSKFTLSSFEQLIYIMNRDIMKLDEIKDLFGEVTVNSEGKDKTYKLKLFDRESFSV
                     PKDKIKDVKFKNIKGSIACMVEALGNKDDLEKNKTDDFSIKIDYSLKDSTQNQMSYNH
                     SDLVKVTIMPSFSKNVITGNYEITYVVPAGFRFIELDRKNPFWVEENGQKLRFFFHYD
                     RQNFAAIPITFYIQAAQKGEYTVDYVVIKENLEPKLNYLDKTILSVK"
     assembly_gap    4418..4517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4518..6733)
                     /locus_tag="PKH42_07585"
     CDS             complement(<4518..6733)
                     /locus_tag="PKH42_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOA20331.1"
                     /translation="MKKLLCLISLMCLILLSCKKTDVLPGLDTSPVEYGVDDKYELIA
                     LKSDKQGIDSTTVFQLTSKEEINNNFIKNNMQIVPEQEYKIEKLSGTTYNIIPAVNLE
                     NDKIYQIVLNDEGYEYSWAFQTKKKFVVESTLPTDRSGYVPSNSGIEIYFSLSGLEEL
                     DDFFEIKPHVDGKFIYKNNTAIFIPDKLEENINYTVTIKKGYGLKDSEQKLEEDYSFS
                     FNTKKEKYAEIYFDRPLTNIYENNAKIIEAYINNENLEFDINIYQYSNADYFAENIND
                     FAETGKFFTGSVDDIEGLSLLNTIKQKPNISYKNIYNTPKGLFEIPGLPKGYYLLEFS
                     TNSGEKNYHFLQINDMLIYYALFENQIVVIASNASDSKGIVDAGVVLNGEHLGFTDND
                     GILIADRDTSKYENKTISLRVEAKGFSDFVYADSFYFFNYYYRSQYEAYKYIRYIDTD
                     RPVYLPTDKINVWGFARHRDDKSVNKVRIELVEANTGLVLDEKTVDLTNIGTYQTEFE
                     IKNITSDILQINVYDNDLKISTKYVSIGEYTKPLYTVKGEFDKKFVYKGESVNYKVKA
                     SFFDGYPVPNLELKFGTFSSNYSGSLEYGGMDTTVKTDEKGETVISVNTNLTSDSWRP
                     VSIRASLWNAKAEDKPVSLIDGFEVFPHHEMVEIVQNDVEHPENISILLHELDITNYD
                     SQYFGGYEKLRGNPLSGTVQVSVAESYYKKVKKGEYYDFINKVNVVDYTYNYVENVV"
     gene            6891..>7636
                     /locus_tag="PKH42_07590"
     CDS             6891..>7636
                     /locus_tag="PKH42_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOA20332.1"
                     /translation="MEYTVKKLSLLAGVSARTLRYYDEIGILKPARINSSGYRIYGKE
                     EVDRLQQILFYRELDIGLDSIKKILSSPDYDDIVALREHKRALLAKREQLNILIDNLD
                     KTIKYREGRIVMNDSEKFEGFKKQLIEENEKKFGQEIREKYGEEAVKKSNEQFMNMSE
                     EQYKEFEELGNSIFEYLKKAMETNDPAGELAQKTAELHKKWITFCWGSYDKEAHAALA
                     QMYVDDERFKENYEKVQKGAAEFLRDAIFI"
BASE COUNT         2397 a         1322 c         1062 g         2755 t
ORIGIN      
        1 ttattttata tttatgcttg cctttttctt cctgcttcca ttcataataa aaagaggcag
       61 gtacaatgca tctgttggat gaagacagtt ttttaaacat tagcttttcg ttaagtgttt
      121 cagccctagc attaattatt acacccttgc cgtcccactt tggaaatccc catttagcta
      181 aaagaggctt tattttatta tcatcctcta tataaatagg agcaatgttt gtaggaaaaa
      241 tttcaccgcc tttaaaattc tcataaatat ttctttgtgc ctcttctaat atttttttga
      301 attcttcatt atcaatatca atgtaatatc ttccgcacat attatcctca tttaaatatt
      361 ttcagagtga atttttttga atatatttat tgtatcgaat acactttcat gaacaaccga
      421 tttaacgtca aatttaagca atttaaatgt agtttgaagt aaaaagccta tgctgaaaac
      481 ataaataacc gttccaagcc caaccttagc tcccaatatc cagcctataa acaaaacagt
      541 tgcttcaatg gagcctctta ctatacctat tggaatatta gaaaattttt tccctaaacc
      601 aatcattaaa gaatccctgg gtcctgctcc aagacccaca ccaatatata aatatgaagc
      661 tatgcatgct aatatttggc ctgttacaag cattaataca cctgtgaaaa aattttgcat
      721 atatggtatt atttttgaat caataattaa atcagaaaag aatcctatca atatagcgtt
      781 taaaattgtc ccgaacccga ttttttcttt taatagaaaa aataatgcta ttacaattgt
      841 aatacctata agaatcgata cattaccgta agatattccc gttactttac tgatgcctat
      901 tgcaaaggca tcccagggag caagtccaat attggcataa atacataaaa ctaaacccaa
      961 agcatatata taaagaccaa atattaatct gataaatttt tttaaatata tctgcatagc
     1021 atcctcctga tttcaataaa ctaattttat atgaatttat attaaataac aacaaatttt
     1081 taaatattgt aaataaaagg tatacaatat gtgtgtggat gatgtcaaag tatcttaata
     1141 aaacaaatat gttatatagc ttgttgtttc ttcgattttc ttttttaata tgagctctga
     1201 attgctgtga tttagtacag tatcaccagc tgctcccaat ttgtccatca tttttagcaa
     1261 ggtgacaaca gatatcggtg agtctaaatt atcattggta aaagacatga gtttgtttat
     1321 gtctttgttt tttatggctt ccatacttat caaatctttt ttttgagcat cttccaaatt
     1381 taaataatgg gaaaaatcaa ctgaggcaat aaacaatgta tcgtgtaagt ttatattttt
     1441 atataattca ttcgtcaata tttcaacatc ctctaatttc acctgctttg tcaaaactaa
     1501 ggtaactatc ttaatatcat tcagataata gttaataaag ggaataatac ttgaggtgga
     1561 atgttccatt gttatcttat cgtcatcttc tgttacaaag tcgtatttta ataattcttt
     1621 tgttaaatta ccgtctgttt ctaagacacc tcttggtgtt tgccaattgc ttaatgtcgt
     1681 aaatattttt cctttattgg tactttcgtg gtcgggacca atcaatacca ctgttttata
     1741 tttatttttt tgcacctttt ggaatacttc atgaattaaa tcttttgcaa ccaaatgatg
     1801 aggtactata catccatgta tagtaccgat atcttgactt gtttcatact caggaatatt
     1861 aataaaatct gatggatcgt aatataaaca ttcaatattt tttcttccgt caaaaaagta
     1921 tttttcatgc ctgtttttcg ctgtattata agcaagtata cttccggcaa tcgtaataaa
     1981 caataaaaat acagtaaata ctttaaaaaa tttattcatt atgcacctgc cacttagatt
     2041 tatccggaat tttatttcac tgataaaatt gtcttatcaa gataattcaa tttaggctcc
     2101 aaattttctt ttataaccac ataatccaca gtatactctc ccttttgtgc agcttgaata
     2161 taaaatgtaa tagggattgc agcaaagttt tgcctgtcat aatggaagaa aaatcttaat
     2221 ttttgaccgt tttcttcaac ccaaaacgga ttcttcctat caagctcaat aaacctgaaa
     2281 ccggcaggaa ccacatacgt tatttcataa tttcctgtta ttacattttt gctaaaggat
     2341 ggcattatgg ttactttaac taagtcggaa tgattgtatg acatttggtt ttgtgtggaa
     2401 tctttaagac tatagtctat ttttatagag aaatcatcgg ttttattttt ttctaaatca
     2461 tccttatttc caagtgcttc aaccatgcat gctatactcc cttttatatt cttaaatttg
     2521 acatccttaa ttttatcctt tgggacggaa aaactttccc tgtcaaataa tttcaattta
     2581 tatgttttat ctttaccttc tgaattaaca gtaacttctc cgaacaaatc tttaatttca
     2641 tctaatttca ttatatctct gttcataata tatattagct gctcaaagct tgaaagggta
     2701 aacttagaag gattattata aatatattta aacaatttgt cgcttgtttc ataatcttta
     2761 agcttgcatc ccaatagcga caatagtgct gttaattcat aattttcgga actactattt
     2821 tcatctgcac cactttcaaa atataaataa tttccggatt ctttaaagct gtttgaaaat
     2881 tctttataat attttttagc cgtatttaag tctcccaatt ccgccaaagc taaggaaaga
     2941 taaattttat ctcttatttc caatgtattg ttttccaata aatcataaat tgttaataat
     3001 accggctcct tataggtgct taagccccaa agagctgccg gaatttcttc gttgtaatcc
     3061 ggattttgca atatattttt aaagtaagtt ttcgacttat accctatata ttcattatct
     3121 atcaagttta taagttttgc ggttatttca acatcagctg cggaatatgt gaaaaggctg
     3181 tatccacccc atgtatcgat ttcatatttg ttgacttcat tgattagttc gtcctcatta
     3241 aaatataaat cggttttaaa ataatcattt atatatttgg ctgccaacat tgaacataca
     3301 gtttggtcaa tcctttttcc gctgttagct aaaatatttt ctaaactctt gtaaaaatct
     3361 gagcggcttt cgttatacaa ggtaattacc gggttagaat atacgctgtc caacacagta
     3421 tcttccgtca atttataata agttgtattg ttaaaatata cgaatgaatc aagaacattg
     3481 aattcttcca ttatcccgtc ttcaaaatcc ttataagaag cataaatata tatttcataa
     3541 gagccttcat ctaatttatc aaatgaaata ttcgtatagt ctcctataat acctgtttcc
     3601 ttatatttca actctttacc ggtttcttta tttataattt taatttcata atttacttct
     3661 tctgattctt ttgcttcagc gccgaatacc cttagggagg cagatatttt atcatccttc
     3721 agatattctt tgctcatgat tacatctacg aagaacggca aacttgcggt aatatttttt
     3781 gtgccgcttc ctgcaaataa tttgtctgaa atcccttgat aggttattct ccaggatgtc
     3841 aaattatccg ctaatttaaa ttttaattct ccattgccgt ttttatctgt tgttatggtt
     3901 ttaaacacat ttgtatcctt gaattcatct ctgaaattag tataatcttc tcctcctccg
     3961 cccctttcag caccactgcc attatccatg cttaaatcaa tattagacag ataagtagca
     4021 acaagtcctg agctaaccct ggcatataaa gaattcaaag tatccactct tttttcgaac
     4081 aatgcaaaat atgcctcatc tacaacggat atatttacat ctgcagtaat tggtttatta
     4141 ttttcatcct ttgtttttat acttaataca acttcttcac cgggtctgta ttcttcctta
     4201 tccgttgtta catcaaagtg tatttgcttt tcggttgtat catacaataa agagcctgtg
     4261 aattccaccg gatacatata cccgttttta taatatatgc tgtttagcat tacattcggt
     4321 atatattctt cttcaaattc aaagctgaga tttgtattgt cagtgatttt ataatctata
     4381 agcccgtttt tcataattaa cagtataagt ttatcatnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnaca acattttcga catagttata tgtataatca acaacattta
     4561 ccttatttat aaaatcgtaa tactcacctt tttttacctt tttataataa ctttccgcta
     4621 cgcttacttg taccgtaccg cttaagggat tacccctcag cttttcatat ccgccgaaat
     4681 attggctgtc ataattggta atgtctaatt catgcaataa tatacttata ttttcaggat
     4741 gctccacgtc attttgcaca atttcaacca tttcatggtg agggaatact tcaaatccat
     4801 cgataaggct cacaggctta tcctctgctt ttgcattcca aagacttgct cgtattgaaa
     4861 cgggtctcca gctgtcgcta gttaaattcg tatttacact tattactgtt tctccctttt
     4921 catcagtttt aacggttgta tccattccgc cgtattctaa acttccgctg tagttactgg
     4981 aaaatgtgcc gaatttaagc tctaagttcg gtaccggata gccgtcaaag aaagaggcct
     5041 ttactttata gtttacactt tcacccttgt aaacaaattt tttatcaaat tcccccttta
     5101 ccgtataaag aggttttgta tattcaccga tggatacata ttttgtggat atttttaaat
     5161 cattatcgta cacatttatt tgaagaatat cagaagtaat attttttatt tcaaattctg
     5221 tttggtaagt tcctatattc gttaagtcga ctgtcttttc atctaacacc aagcccgtat
     5281 tagcttcaac caattcaatt cttaccttat taacagactt atcatcccta tgccttgcaa
     5341 atccccatac attgatttta tccgtaggca ggtagacagg tctgtccgta tcaatatacc
     5401 ttatatattt gtaagcttca tactgacttc tgtaataata attgaaaaaa taaaagctgt
     5461 ctgcatatac aaaatcgctg aaacccttag cctcaactct taaagaaatt gttttgtttt
     5521 catatttaga agtatctcta tccgctataa gtatgccgtc attgtcggta aaacctaaat
     5581 gctctccgtt caataccaca cctgcatcca caataccctt gctgtcagat gcattcgatg
     5641 caatgacaac aatttgattt tcaaataaag cataataaat tagcatatcg ttaatttgca
     5701 ggaagtgata atttttttca cccgaattcg ttgaaaattc caataaataa tatccctttg
     5761 gaaggccggg aatttcgaat aatccttttg gagtattata aatattctta taagaaatgt
     5821 ttggcttttg ctttatagta tttaacaggg ataatccttc aatatcatca acactgccgg
     5881 taaaaaattt gcctgtttct gcaaaatcat tgatattttc tgcaaaatag tctgcatttg
     5941 aatactgata aatatttatg tcaaattcca gattttcatt atttatatat gcttcaatta
     6001 tttttgcatt gttttcgtaa atatttgtca atggcctgtc aaaataaatt tcagcatact
     6061 tttctttttt tgtattaaag ctgaaggaat aatcttcttc taatttctgc tcactgtcct
     6121 tgagaccata accttttttt attgttaccg tataattgat gttttcttct aatttatcgg
     6181 gaatgaaaat agcagtatta tttttgtaga taaatttacc atccacatga ggcttaattt
     6241 caaaaaaatc atccaattct tctaaaccgc ttaagctgaa atatatttca atacctgaat
     6301 ttgaaggtac ataaccgctt ctatcggtag ggagggtact ttccactaca aattttttct
     6361 tagtttgaaa tgcccaggag tactcatatc cttcatcatt gagtacgatt tgatatatct
     6421 tgtcgttttc aagatttaca gcaggaataa tattatatgt tgtacctgaa agtttttcaa
     6481 ttttatactc ttgctcggga actatttgca tattattttt tatgaaatta ttgtttattt
     6541 cttcctttga agtcagctga aaaacagttg tactgtcaat tccctgttta tctgatttaa
     6601 gggctataag ctcgtattta tcgtcaacac cgtattcaac cggcgaagta tctaatccgg
     6661 gcaacacatc agtcttcttg caagacaaca aaataaggca cattaatgag attaagcata
     6721 ataatttttt catttcttcc ctcccaaaga ttatatgtca attatatcac ttatcatatg
     6781 gtgttacaag acagttcaag aaatccggaa taaataatct ttttatagaa ttaatatatt
     6841 gacattgacg ttacgtaaag gtatacaata tatttgtaag gagtgaaatt atggagtata
     6901 cagttaagaa acttagtttg ctggcaggtg tgagcgccag gaccttaagg tattacgatg
     6961 agataggaat tcttaagccg gcaagaatta actcatccgg atatcgcatc tacggaaaag
     7021 aggaagtaga caggctgcag caaatattgt tttacaggga attagatatt ggtttagaca
     7081 gtataaagaa aatactttct tcacctgatt atgatgatat tgttgctctt agggaacata
     7141 aaagagccct ccttgcaaag agagaacaat taaatattct aattgataat ctggataaaa
     7201 cgattaagta tagagaaggg agaattgtta tgaatgatag cgaaaaattt gaaggattca
     7261 agaaacaatt aatcgaagaa aatgaaaaga aattcgggca ggaaataaga gaaaaatatg
     7321 gtgaagaagc cgtgaaaaaa tccaatgaac agtttatgaa catgtctgag gagcaatata
     7381 aggaatttga agaattaggc aattcaattt ttgaatatct taaaaaggca atggaaacaa
     7441 atgaccctgc aggagaactt gcacaaaaaa cagctgaact tcataagaag tggattacct
     7501 tttgctgggg ctcttacgac aaagaagcac atgctgctct tgctcagatg tatgtggatg
     7561 acgaaagatt taaagaaaat tatgaaaagg tacaaaaagg agctgcagaa tttttgcgtg
     7621 atgcaatatt cattta
//
LOCUS       DAOPUE010000084         7326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10119_length_7326_cov_9.607619, whole genome shotgun sequence.
ACCESSION   DAOPUE010000084 DAOPUE010000000
VERSION     DAOPUE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10119_length_7326_cov_9.607619"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PKH42_07595"
     CDS             <1..222
                     /locus_tag="PKH42_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20333.1"
                     /translation="MDILIGIFIFIVFIVAALKVGVVLLKVIFTFLGAIIGFVVILAL
                     IPLGIGLLLIPAIIIGIIVAIVKCIKFIF"
     misc_binding    275..505
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            541..>1889
                     /gene="metG"
                     /locus_tag="PKH42_07600"
     CDS             541..>1889
                     /gene="metG"
                     /locus_tag="PKH42_07600"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOA20334.1"
                     /translation="MDKGKFYLTTAIAYTSGKPHIGNTYEIVLADSIVRFKKLNGYDV
                     YFQTGTDEHGQKIQQKAEEAGKSPQEFVDEVAAEIKRIWDLMNTTYDKFVRTTDPEHV
                     KIVQKIFKKLYDRGDIYKGYYEGWYCTPDEAFFTDAQLVDGKCPDCGREVHMAREEAY
                     FFKMSNYADRLMKHIEENPKFIQPESRKNEMVNNFIKPGLQDLCVSRTSFNWGIPVTF
                     DEGHVVYVWIDALSNYITFLGYDPDGCHGELFNKYWPADVHLIGKDILRFHTIYWPIL
                     LMALDVELPKQVFGHPWLLVGEGKMSKSKGNVIYADDLVELFGVDAIRYFVLHEMPFA
                     SDGTLTYELVIERINSDLANILGNLVNRTISMTNKYFGGEVQAPRASEPIDDELINLA
                     LQTPLKVEKKMDELKVSDSIDEIFTLLRRSNKYIDETQPWILGRDENKKERLGTVLY"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2233..2619)
                     /locus_tag="PKH42_07605"
     CDS             complement(2233..2619)
                     /locus_tag="PKH42_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HOA20335.1"
                     /translation="MCNKGKFFICETCGNLVGLVLEGGGELVCCGAPMKELVANTEEA
                     AYEKHIPEVKVEGNKVTVQVGSVLHPMVEEHYIQFIYLVTKEGAQKVCLCPGDKPIAE
                     FALVEGDEVVAAYEFCNLHGLWKKEQ"
     gene            2845..3375
                     /locus_tag="PKH42_07610"
     CDS             2845..3375
                     /locus_tag="PKH42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOA20336.1"
                     /translation="MREKEVKVLNIDKEAIEKKLVDMGAVLVKDENQINYRFDTNDYF
                     LKKTYNGYLRIRIIKDNLSGETKSTMTFKRSIKRDSLRVNEEINTDISDWKSTAKIIE
                     LLGYEQKRPGYKHRRSYMYENILFEIDTWDEQTYTKPYLEIEVTSDEELEKAIVLLNL
                     DRSQITTKPIDELRKD"
     gene            3402..4181
                     /locus_tag="PKH42_07615"
     CDS             3402..4181
                     /locus_tag="PKH42_07615"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOA20337.1"
                     /translation="MVLVVDIGNTNIVIGVYDGNELVGNWRLVTRDEKTSDEYGISIL
                     SLLRYNDINHKNINSAIVSSVVPNVMHSFENALKKFFHIDPIIVGPGVKTGITIITDN
                     PREVGAGRIVNIVAAHELYGNGDTIMAIDFGTATTYDIVNEKGEFRYGITSPGIQISA
                     DALWQRTAQLPKIEIKKPDSILGKNTVMSMQAGLIYGYIGQVEYIIKKVKEELHNENI
                     KVVATGGLGRLIYDGTSEIDIFDPHLVFKGLKIIHDKNYKK"
     assembly_gap    4228..4327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4328..5551
                     /locus_tag="PKH42_07620"
     CDS             <4328..5551
                     /locus_tag="PKH42_07620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOA20338.1"
                     /translation="MHIDVNSAFLSWTAAYEKQMGIERDVRTVPSVIGGNEQSRHGIV
                     LAKSSPAKKFKIQTGMSLMEARQMCPNLLVIPPNYSVYVRASKALREYLKKYTPDVEV
                     FSIDECFLRFTDINREQAVKLAHKIKDGVREKFGYTVNVGISENKLLAKQAGDLEKPD
                     KCHTMFIEEIKEKLWPLPVEELFMVGRRTKPKLNRWGIYTIGELANADYKLISTMLKS
                     HGRLIYNYAWGRDISIFKERAPIKSVGNSSTLRFDVTDRETAHVVLLSLTEMTAWRLR
                     EANMNCRVVSISIKDKDFGFKIKQRKLMYFTDCTRDIYMNACSLFDELWDKKPIRALG
                     VHVSDLEFSEVKQINFFQDKLSLKNEILDKAVDKIRAKYGDNSVIRGVFANSDLKPIL
                     GGYPDDEYPGMSSIL"
     gene            5562..5801
                     /locus_tag="PKH42_07625"
     CDS             5562..5801
                     /locus_tag="PKH42_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20339.1"
                     /translation="MKILNEPIKVMAVFNTNGKIEPVKFRLDDKVVRIEKIIKTYEEN
                     IVGNTRIVFVCLHKGKDIFELKYEVASKTWYLFKK"
     gene            5952..6683
                     /gene="sigI"
                     /locus_tag="PKH42_07630"
     CDS             5952..6683
                     /gene="sigI"
                     /locus_tag="PKH42_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HOA20340.1"
                     /translation="MSDLDLLAVEAKTNTDLMNNLIIQYENFILKCASSTTRSYISKS
                     DDEWSIALSAFTEAIKNYSADKGSFLNFAELVIRRRMIDFLRSKSRYAPEVLVNPSLF
                     NTDSQDEEEYSLQSEIAKKVSIVENNSIKMEIALINEIFSDYGFSFMDLTECSPKSHK
                     TKKSCAKVVAYLIKNPILTREIKIKKQLPLNIIEKYTNVPRKLLERHRKYIIAALEIM
                     SGDFPNLAGYMEYIRQELKNESNNS"
     gene            6664..>7326
                     /locus_tag="PKH42_07635"
     CDS             6664..>7326
                     /locus_tag="PKH42_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HOA20341.1"
                     /translation="MKAIIVEIKGRHAAVLTDDGVVSKIKNRNYCIGQEIVIKNNRNK
                     LIRMTAAAAAAIMLFVTPAWAYLTPYSYVSIDVNPSFEFLINRFDRVLTVRGFNDDGR
                     ELSKDINIDGLKNKEVQNAVKSVLNELKNKGYIIEGQEGGVIIATSSKSEAKKDVLFE
                     KLRTAVNEEEAPEQTKDNEIKTKIEEPKEPESTEKLEEKEESEAQQTETIEKTEELTD
                     DKS"
BASE COUNT         2591 a         1025 c         1429 g         2081 t
ORIGIN      
        1 atggatatat taattggcat atttatattt atagtattta ttgttgcagc gttaaaagta
       61 ggtgttgtat tacttaaggt tatatttaca tttttaggtg caataatcgg atttgtcgtg
      121 attcttgcat taattccttt aggaatagga cttttgctga ttcccgcaat aataatcggg
      181 ataatagttg caatagtcaa atgcattaaa tttattttct agttgacaaa aattagttat
      241 ttatgtatag ttttaaatat gatatacata ataaagaaaa gcgatgataa gaagagtagt
      301 tttaacaatc atgatataga gaaccgggta aggtggaagc cggagattga ttttaaaatg
      361 aacatggcct tggagcttcg tgcctgagcg atagttaggg tacgacgtaa gtatacgtta
      421 taatacaggg ttttattgaa cctgttgagg cacatattgt gaaatatgtg taaaaaagag
      481 tggtaacgcg ttattcgcct cttagcttat gctaagaggc gttaatatta ggaggaaaat
      541 atggataaag gcaaatttta tttaacaact gcaattgcat atacatcagg caaacctcat
      601 atcggaaata catatgaaat agttcttgcc gacagtattg taagattcaa gaaattaaac
      661 ggatatgatg tatattttca aacgggaaca gatgagcacg gacaaaaaat tcagcaaaaa
      721 gctgaggaag caggcaaatc tccacaggaa tttgttgatg aagtagcagc tgaaattaaa
      781 agaatatggg atttaatgaa tactacttat gataaattcg taagaaccac agaccctgag
      841 catgttaaaa tcgttcaaaa aatatttaag aaactgtacg accggggaga tatttacaaa
      901 ggttattacg aaggatggta ctgtactccc gatgaagctt tctttacgga tgctcaatta
      961 gttgacggca aatgccctga ctgcggaaga gaagttcata tggcaaggga agaagcatat
     1021 ttctttaaaa tgagcaatta tgccgacaga ttgatgaagc atattgaaga gaatcctaaa
     1081 ttcatccagc ctgaatcaag gaaaaatgaa atggtaaata attttattaa accgggactt
     1141 caggatttgt gcgtatcaag aacatctttt aattggggaa ttccggtaac atttgatgaa
     1201 ggtcacgttg tatatgtatg gattgatgca ttaagcaact atattacatt tttgggatat
     1261 gaccctgacg gctgtcatgg agagcttttt aacaaatact ggccggcaga tgtacatttg
     1321 attggaaagg atattttaag attccacact atttattggc ctatactttt gatggcatta
     1381 gatgtagaac ttccaaagca ggtttttggt cacccgtggc tcttagtcgg tgaaggcaag
     1441 atgagcaaat caaaaggaaa tgtaatttat gcagacgact tggtggaatt attcggggtg
     1501 gatgcaataa ggtactttgt tcttcacgaa atgccctttg caagtgacgg taccttgact
     1561 tatgaattgg taatagaaag aatcaattct gacttggcta acatattagg taatttagta
     1621 aacagaacaa tatcgatgac caataaatat ttcggagggg aagttcaagc tccaagggca
     1681 tctgagccga tagatgatga actaattaat ctggcccttc aaacaccttt gaaggttgaa
     1741 aagaagatgg atgaattaaa agtatcggat tccatagatg aaatatttac actgcttcga
     1801 agaagcaaca aatatatcga tgaaacacag ccttggattc tgggaagaga tgaaaacaaa
     1861 aaggaaagat taggaacagt tttgtataan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnc caagcggagc aacaatatca taatatacct ttatgaccta acccaaaaaa
     2041 ttaaaggatt tgaaaagtaa ggcaccttac tctcaaatcc ttttttgaat ggggacaagt
     2101 atggggactt agggacattc ctcttacttc aaagattatc cctaagcaat aataggtgtg
     2161 tctccataca agtacaggga cattcctctt actccaaaga cttaccctag agagaggtgt
     2221 gtccctcttc ctttattgtt ctttcttcca aagtccatgg agattgcaga attcataagc
     2281 tgcaacaact tcatctccct caaccaaagc aaattcagct atcggcttat ctccgggaca
     2341 caaacatact ttctgggcac cttccttagt tacaaggtaa ataaattgaa tatagtgctc
     2401 ttcaaccatc gggtgaagaa cgcttccaac ttgaacagtt accttgtttc cttcaacctt
     2461 aacttcaggt atatgctttt cataagcagc ttcctcagta tttgcaacaa gttccttcat
     2521 tggggctccg cagcaaacta attcccctcc gccttccaat accaatccta ctaaattacc
     2581 gcaagtttca caaataaaga attttccttt attacacata taatacctcc taagttttat
     2641 tcttcaacat taagtatata ccctttataa atatatttaa aacaaaatta gcttattttt
     2701 ttaattaatt attgataccg atttatgata catcactttc taatattgat tgataaaagc
     2761 aatattaatt tttagcaaac gataataaat gtttattatt ctaaataatt atgatataat
     2821 tacctaaatc taatggagga cataatgaga gagaaggaag taaaagtttt aaatattgac
     2881 aaagaagcaa tagaaaaaaa gcttgttgat atgggagctg ttcttgtaaa ggacgaaaat
     2941 cagattaact atagatttga tacgaatgat tattttttaa agaaaactta caacggctat
     3001 ttgagaataa gaataataaa ggataattta tcgggagaaa ccaaaagcac catgacattt
     3061 aagaggagca taaaaagaga ctccttaaga gttaatgagg aaatcaacac ggatatttct
     3121 gactggaaaa gtactgctaa gataatcgag ctattgggat atgagcaaaa aagacccggc
     3181 tataaacaca gaagatctta tatgtatgaa aatatattgt ttgaaattga tacatgggat
     3241 gagcaaacct atactaaacc ttatcttgaa attgaagtga cttccgatga agaattggaa
     3301 aaagcaatag ttttgttaaa tcttgacaga agtcagatta caacaaaacc catagatgag
     3361 ctgaggaaag attaactata gataatatag gaggaggaat tatggtatta gttgttgata
     3421 tcggaaacac aaatatagta atcggagttt acgacggtaa tgaacttgtg ggaaactgga
     3481 gattagttac cagagatgaa aagacatccg atgaatatgg aatttctata ttaagtttat
     3541 tgagatacaa tgatattaac cataaaaata ttaacagtgc aatcgtatcc tcggttgtac
     3601 ctaacgtaat gcattctttt gaaaatgcct taaaaaagtt ttttcatatt gaccccatta
     3661 ttgtaggtcc cggggtgaaa acgggaatta caataataac cgataatcct cgagaggtag
     3721 gagcaggcag gattgtgaat atcgtagcag ctcatgagtt atacggcaac ggtgatacca
     3781 taatggctat agactttgga acagcaacaa cttatgatat tgtaaatgaa aagggagaat
     3841 ttagatatgg cattacatct cccggaatcc agatttctgc agatgctttg tggcaaagaa
     3901 ctgcacagct tccaaaaatt gaaattaaaa aacctgattc aattcttggc aaaaatacgg
     3961 taatgagtat gcaggcagga ttaatctacg ggtatatagg acaggtagaa tatattatta
     4021 aaaaagtaaa agaagaattg cataatgaaa atattaaagt tgtagctaca ggaggacttg
     4081 gcaggttaat atatgatgga acctcagaaa ttgatatctt tgaccctcat ctggtgttta
     4141 aaggattaaa aataattcac gataaaaatt ataaaaaata atatattgat aaagatttgt
     4201 aatagtttac agatcttttt ttatgtannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnatg catattgatg taaattcagc atttttaagc tggacggctg catatgaaaa
     4381 acaaatggga atagaaagag atgtacggac agtgccgtct gtcataggag gaaacgaaca
     4441 aagccggcat ggaattgttc ttgccaaatc aagtcctgcc aaaaaattca agatacagac
     4501 gggaatgtcc ctcatggaag caagacaaat gtgtcctaat ttactggtta tacctcccaa
     4561 ttacagcgtt tatgtaagag caagcaaagc tctcagggaa tatttgaaaa aatatactcc
     4621 cgatgtagaa gttttcagca ttgatgaatg ttttttaaga tttaccgaca tcaatagaga
     4681 gcaggctgtt aaacttgccc ataaaataaa agacggcgtt cgggaaaaat tcggttatac
     4741 cgtaaatgta ggaatttctg aaaataagct tcttgcaaaa caagcaggag atttagaaaa
     4801 gcccgacaag tgtcatacca tgtttattga ggaaataaag gaaaaattat ggcctttgcc
     4861 tgttgaagag ctatttatgg ttggtagaag aacaaagcca aaattaaaca gatggggtat
     4921 ttatacaata ggagagcttg ccaatgcaga ttataaatta atatcaacaa tgcttaaatc
     4981 tcacggcaga ctaatttaca attatgcctg gggaagggat atttcaattt ttaaagaaag
     5041 ggccccgatt aaaagtgtag gaaacagcag tacccttaga tttgatgtta cagacaggga
     5101 aactgctcat gttgtattgc tgagtttgac tgaaatgacc gcctggagat taagagaagc
     5161 aaatatgaat tgcagggttg taagcataag tattaaagac aaggatttcg gttttaagat
     5221 taagcaaaga aagcttatgt attttacaga ctgtacaagg gatatttata tgaatgcatg
     5281 cagtttattt gatgaattat gggataaaaa gccgataagg gcattggggg ttcatgtatc
     5341 ggacttggag ttttcagagg ttaaacaaat taattttttt caggataaat taagtttgaa
     5401 aaatgaaatt ttagataagg ctgtagataa aatacgagct aagtatggcg ataattccgt
     5461 tatcaggggg gtttttgcca acagtgattt aaaacctatc ttaggggggt atccggatga
     5521 tgaatatcct ggtatgagca gtattttata gaggtggtac tatgaaaatt ttaaatgagc
     5581 ccataaaagt aatggctgtt tttaatacaa acggaaaaat agagcctgta aaattcaggc
     5641 ttgatgataa ggttgtgagg attgaaaaaa taattaaaac ttatgaagaa aatattgtcg
     5701 ggaacacaag aattgtattt gtatgtctcc ataaggggaa ggatattttt gagcttaaat
     5761 atgaggtagc cagcaagaca tggtatcttt ttaaaaagta attcttcctg ccggcagggg
     5821 ctattccaaa ataggaaggt gtgtccccct tcctttgttt ccccattaag aattttgtaa
     5881 gatttgaaat gttgtaatat tatggtctga atgatataat gttttgtaat tgtaactaca
     5941 aggggaagaa catgagcgat ttggacctat tggctgttga agctaagact aatacagatt
     6001 taatgaataa cctaataatt caatatgaaa atttcatact taaatgtgcc tcatctacta
     6061 cccgaagcta tatttcaaaa agtgatgatg agtggtcaat agccttgtct gcttttactg
     6121 aagcaattaa aaattattct gccgataagg gcagtttttt aaactttgca gaattggtaa
     6181 taagaaggag aatgattgat ttcttgcgca gcaagtcaag atatgcgccg gaagttttag
     6241 taaatccttc attgtttaat actgattctc aagatgaaga agaatactcc ttgcagtccg
     6301 aaattgcaaa aaaggtttcc attgttgaaa ataattcaat taagatggaa attgccctca
     6361 tcaatgaaat tttttccgat tatggatttt catttatgga tttgaccgaa tgctcgccaa
     6421 aatctcacaa aactaagaag tcctgcgcta aggttgtggc ttatttaata aaaaatccaa
     6481 tattgacaag agaaataaaa attaagaagc aattgccatt aaatataata gaaaaataca
     6541 caaatgtacc ccgtaaattg ttggaacgcc atcgaaaata tattatagca gcattagaaa
     6601 tcatgtcggg ggattttccg aatttagcag gttatatgga atatattcgt caggagttga
     6661 aaaatgaaag caataatagt tgaaattaaa ggaagacatg cagcagtatt gactgatgac
     6721 ggtgttgttt ctaaaattaa aaacagaaac tattgcatag gtcaagaaat agtaataaaa
     6781 aataacagaa ataaattaat cagaatgacg gctgcggcag ctgctgcaat catgttattt
     6841 gttacacctg catgggcata cttgactcct tattcctatg taagcataga tgtaaatcca
     6901 tcttttgaat ttttgataaa cagattcgac agggttctga ctgtgagggg ttttaatgat
     6961 gatggaagag aattgtcaaa agatataaat attgacggtt tgaaaaataa agaagttcaa
     7021 aacgcggtaa aaagtgtatt aaatgaatta aaaaataagg gatatattat agaaggccaa
     7081 gaaggcggcg taataattgc cacatcaagc aagtcagaag caaaaaaaga tgtgttgttt
     7141 gaaaaacttc gaactgccgt taatgaggaa gaagcaccgg aacagacgaa agataatgaa
     7201 attaaaacaa aaattgaaga gcccaaggag ccggaaagta cggaaaaact agaagagaag
     7261 gaagaatctg aagcacagca aacagaaaca attgaaaaaa ctgaagaact tactgatgat
     7321 aaatca
//
LOCUS       DAOPUE010000085         7313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10150_length_7313_cov_8.302425, whole genome shotgun sequence.
ACCESSION   DAOPUE010000085 DAOPUE010000000
VERSION     DAOPUE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10150_length_7313_cov_8.302425"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..679
                     /locus_tag="PKH42_07640"
     CDS             251..679
                     /locus_tag="PKH42_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA20342.1"
                     /translation="MLNKGFTLIELVVAISILCIVSMLAVAIIDIDMFYLEKMADEFA
                     MDVRYVQMECMKGETDNHRICIDITNGCYHIYNVAVIKKTVVFKNRYKIDYSNQNMSV
                     GFTHDGTPVNPGTFTIIDTKTNETLKISIVPTTGRTVIKE"
     gene            722..1279
                     /gene="efp"
                     /locus_tag="PKH42_07645"
     CDS             722..1279
                     /gene="efp"
                     /locus_tag="PKH42_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOA20343.1"
                     /translation="MITAGDFKKGITLSWNNGLWQVVDFQHVKPGKGAAFVRAKLKNI
                     VTGTIKEETFNPTEKFQLAHIETKDMQYLYNDGQLYYFMDPETYEQIPLNYEQVEEAI
                     VYLKENDFAQMRFYEGNPFEVSAPNFVELEIVETEPGFKGDTATGANKPATTETGAKI
                     MVPLFVEIGDKVKIDTRTGEYLSRV"
     gene            1291..1665
                     /locus_tag="PKH42_07650"
     CDS             1291..1665
                     /locus_tag="PKH42_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20344.1"
                     /translation="MDFLYEKISYLRGLADGLEVKDDTKEGKLFYALMEVIEEIADSL
                     SDIVEEQDEVNEYLDLLDEDLSVVEEELFGDLELYEDDDDYDYDDDDDDYDFDFDEDD
                     EENYDDEFEIEEHPCDCAKLED"
     assembly_gap    1747..1846
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2000..2869)
                     /locus_tag="PKH42_07655"
     CDS             complement(2000..2869)
                     /locus_tag="PKH42_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20345.1"
                     /translation="MKEKKYKSNQKKALIAFITIIIIIAAIATVEKLIEPMGYFRPDY
                     DKRDITGILEKEVLSDDDYKELFYQTGLGKTAVDEILKDKSGKEKILEFQKNFFTEYN
                     ISSEKFAVIVNHESLVDEKGKIIYGFDLAPYENGYVLITKATHSLGWRHGHAGIITDA
                     ENEETLEAVLLGQDSMLQNINKWRSFPSFIMLKLKDASQEELDKIAEFAKINLLDVPY
                     GLSVGLTSKKNPEPSNITTTQCAHIVWYPFMQFGYDIDSDGTWLVTPKDIANSDLFEI
                     VQIYGVDPDEIWP"
     gene            3379..5235
                     /locus_tag="PKH42_07660"
     CDS             3379..5235
                     /locus_tag="PKH42_07660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOA20346.1"
                     /translation="MKKIKGLELSSQQINAASFDKGNALVLSTAGSGKTTVIISRAGR
                     LLFENKCSKKILTITFSKMAANDMKKRFACIGNCQNYETKTEFSTIHAFSYKIVRDFY
                     RRKGINFGLLTNNYQALSEILKNHYSKSYFSYVSDEEIENLSSAIGYVNNMMIAPEEY
                     KDYGIEIKGFDKLYNDYKMYKSQNRLIDFDDMLDYSYKILSDYANYKEMILNTFEYVQ
                     IDEMQDTSKIQHELIKLITRDNLFMVGDDDQAIYSFRGSYPDFMLKFKDIFNDGKIFY
                     LDNNYRSDKYIVEGARNFIEINKNRYKKAIKTENSKENEINIIKVKDRAEQAKLITDI
                     VAKAKYENKTIGILYRYNMSAMIPANNLYENNIDFYIKEDKTKFFSSAVLYDILAFLN
                     LAINPKDKDSYARIYYKSYTYFTKGMCKMVLESNEDKSVLDILYGYKYFDYNVYDKIK
                     QFKTDIDYLNKLKPSNAIRFIKFDLEYLNYLERMQEEGRYSLSTSLHILEILEEIGSY
                     CSDIKEFLRKINNLRDVITRASENGNALVTLTTIHSAKGLEYDYVYMIDNLNELNIDK
                     NLSVEDYQSKLEEERRIFYVGMTRAKEKLNIIVPGEPSIFVNELIISNKCMH"
     gene            5350..6537
                     /locus_tag="PKH42_07665"
     CDS             5350..6537
                     /locus_tag="PKH42_07665"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HOA20347.1"
                     /translation="MKFYISTLGEFDIKVDGQSILRDSNRMYRIYKLFEYFLTFRNKK
                     LLPETIIDNLLSDSESEDPKNMLRTQIFRLRKIINALIPKGELPEKYMNLNFTNGYYC
                     LEIGENTIVDIDVFENLIQKGDKEREYDIESAIESYQNAISLYKGLYLSDNAYEVWLV
                     PTRNYYQRLFLKTLNKYIDLLKRNEENEKIVNLCEKSLLIEPFEESIHIELIEALLKI
                     GQNKSAMQHYEYALNMLEKELDAKPSAKFINYINIIQNYTSRNNDLDIEAINKSLEEE
                     LPEGAMYCSLEYFKFLFNLQKRKSKRNNEYDYLLSIMINSNNNKSINDVFSVLKKSLR
                     KGDVFTSWNDNQILVMLHNVKENGTETIKERLTDNLINYTRINRNEIRLIFQPVSMEN
                     TIL"
     gene            6745..>7313
                     /locus_tag="PKH42_07670"
     CDS             6745..>7313
                     /locus_tag="PKH42_07670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOA20348.1"
                     /translation="MEYPSKYDFYLKFKPVYNKEGYFLGYVLIYVSDFFINDINIGAD
                     LTIGKKFSEIVVDSDILGLKEFYFNIIPKSKMKLELYNEDLKRWYLINAFTDMSNRDE
                     HLIIHYADITEIKQNQQTVSLDNNIYYLNDRERLLYKDKLTGLYNKTYFEEELSRLDT
                     KRQLPISLIMGDINGLKLINDAFGHSMGD"
BASE COUNT         2611 a         1025 c         1370 g         2207 t
ORIGIN      
        1 ttcttcgggc taaagccctc agaatgacat aaagaaagga ttgtcatcct gagcgcagcg
       61 aaggatctca tgagattctt cgggctaaag ccctcagaat gacaccctac gggttgtccg
      121 ctgagatgac aagggtgtca tcctgagcga tagcgaagga tctcataagg cccgactaaa
      181 tttatataaa attcattgac aaacacccaa ataatttata gaataaacta tggtatattt
      241 taggtaaact atgctaaaca aaggttttac attaattgaa ttagttgttg caatttcaat
      301 tctatgcatt gtatcgatgt tagccgtggc aattattgat attgatatgt tctacttgga
      361 aaagatggct gatgaatttg ctatggacgt aagatatgtg cagatggaat gcatgaaggg
      421 tgaaaccgat aaccacagga tatgcattga tattaccaat ggatgttatc atatatacaa
      481 tgttgctgtt ataaaaaaaa ccgtagtatt taaaaacagg tataaaatag attattcaaa
      541 tcaaaatatg tctgtcggat ttacgcatga tggtacccct gttaatccgg gaacttttac
      601 cataatagat acaaagacaa acgaaacttt gaaaatatcc attgttccca ctacaggaag
      661 aacggttatt aaggaataag cagcaatctg taggttataa tatacataag gaggaagcaa
      721 attgattaca gcgggcgatt ttaaaaaggg aattacttta tcctggaata acggattgtg
      781 gcaggttgtt gatttccagc acgttaaacc gggaaaggga gctgctttcg ttagggcaaa
      841 attaaaaaat attgtgacag ggactataaa agaggaaact tttaacccaa ccgagaaatt
      901 ccaattggcg catattgaaa ctaaggatat gcaatattta tataatgacg gacaattgta
      961 ttattttatg gaccccgaaa catatgaaca aattccgcta aattatgaac aagtggaaga
     1021 agctattgta tatttgaagg aaaatgactt tgctcaaatg agattctatg aaggaaatcc
     1081 gtttgaagta agtgcgccta actttgttga gttggaaatt gttgaaacag agccgggctt
     1141 taagggtgat accgcaacag gagctaataa gcctgcaaca actgaaaccg gtgcaaaaat
     1201 aatggtgccg ctttttgttg aaatagggga taaagttaag attgacacca gaacaggtga
     1261 atatttgtca agagtataag gaggtataag atggattttt tatatgaaaa aatatcatat
     1321 ttaagaggtt tggctgacgg tctggaagta aaggatgaca caaaagaagg caagttgttt
     1381 tatgcattga tggaagtaat tgaagaaatt gctgattcat tgagtgatat agttgaagaa
     1441 caagatgagg taaatgaata tttggattta ctggatgaag atttgtctgt agttgaagaa
     1501 gaattattcg gcgatttgga attatatgaa gatgatgatg attatgatta cgatgacgat
     1561 gatgatgatt atgacttcga ttttgatgaa gatgatgaag aaaattatga tgacgagttt
     1621 gaaattgagg aacatccctg cgactgtgct aaactagaag attaataaca aatgtacata
     1681 agaagaacat ttctttaggg agatgttctt tttcttatga gattcttcga gctaaagctc
     1741 tcagaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgac aaaatgttat
     1861 tctgagcaat aacttatagt catcctgagc ggagcgaagg atcacatgag attcttcggg
     1921 ctaaagctct cggaatgaca aaatgttatt ctgagcaata acttatagtc atcctgagcg
     1981 gagcgaagga cctcatatat tatggccaga tttcatcggg gtcaaccccg tatatttgaa
     2041 ctatctcaaa caaatcactg tttgcaatat cttttggtgt cacaagccaa gtaccgtccg
     2101 aatcaatatc gtatccgaac tgcataaatg gataccaaac aatgtgagca cactgggtag
     2161 ttgttatatt gctcggttca ggattttttt tgcttgttag tcctacagaa agcccgtagg
     2221 gaacgtctaa taaatttatt ttggcaaatt cagctatttt atctaattct tcctgagatg
     2281 cgtcttttaa tttaagcatt ataaaagaag ggaaacttct ccacttatta atattttgaa
     2341 gcattgaatc ctgacctaat agtaccgctt ccaaagtttc ttcgttttct gcatctgtta
     2401 tgattcctgc atgaccatgc ctccagccta atgaatgggt tgcttttgtt atcaatacat
     2461 atccgttttc atagggagca aggtcaaatc cgtatattat ttttcctttt tcatcaacaa
     2521 gagattcatg gtttactatt actgcaaatt tttcgcttga tatattgtat tccgtgaaaa
     2581 aatttttctg aaactcgagt attttttcct tgccggactt atctttcaaa atttcatcaa
     2641 cggctgtttt accgaggcct gtttgataga acagctcttt gtagtcatcg tcacttaata
     2701 cttccttttc caatataccg gtaatatctc ttttatcgta atcaggacga aaatagccca
     2761 ttggctcaat gagcttttca actgttgcta ttgcagcaat tataataatt attgttataa
     2821 aagcaataag tgctttcttt tgatttgatt tatacttttt ttctttcata gtttctacca
     2881 ttgtttctta tagtgtctta cgtggagagt cattctgagg gctagcccga agaatctcat
     2941 gagatccttc gcttccgctc aggatgacaa aaccgagatc cttcgcttcc gctcaggatg
     3001 acaaaaccga gatccttcgc ttccgctcag gatgacaaag cggattctac tatattatat
     3061 aaataataag taaaaattac aatggcaaag gggcattttt tatgttacaa tatgtatata
     3121 agtaagttgg aggaatgtac atgaacaaat ataaaaaagc tgatagaatg aagagagtta
     3181 tttgtttaat cttggccgta ggaatggttt taaccgcatt tatgagttta ttggcatact
     3241 ttttttaaat agtataattc ttatatatgg gacgtttaaa aaccgtaccc gtcaggttgc
     3301 gaagaattgt cactctgagc aaagcaaacg agcctcaaac gtctctaatt gtggtgatat
     3361 atggagtact ttgattattt gaaaaaaatt aaggggttag aattaagcag ccaacagata
     3421 aatgccgcct cttttgataa aggaaatgca ctggttcttt ccactgcagg ctcaggcaag
     3481 actaccgtta tcatttcacg ggcaggaagg cttttatttg agaataaatg cagcaaaaaa
     3541 attctaacca ttacattttc taaaatggca gccaatgaca tgaagaaaag atttgcatgt
     3601 attggaaatt gtcaaaatta tgaaacaaaa actgaatttt caactattca tgccttttct
     3661 tacaaaattg tgagggattt ttacaggagg aagggcatta attttggatt gcttacgaat
     3721 aattatcagg ccctttcgga aatacttaaa aatcattatt ccaaatcata tttcagctat
     3781 gtgagtgatg aagaaattga aaatttatct tctgcaatcg gatatgtgaa caatatgatg
     3841 attgctcccg aagaatacaa agattatgga atagaaataa aaggttttga taagctttac
     3901 aatgattaca aaatgtacaa gtcacaaaac agattgattg attttgacga catgctggac
     3961 tattcatata aaatattatc agactatgca aattacaaag agatgatttt aaatacattt
     4021 gaatatgtgc agattgatga aatgcaggat acttctaaaa ttcagcacga gctgattaag
     4081 cttataacac gtgacaattt atttatggta ggagatgatg accaagccat atattcattc
     4141 aggggaagct atccggattt catgcttaag tttaaagata tttttaatga cggtaaaatt
     4201 ttttacttgg acaacaatta ccgctcagac aaatatattg tggaaggtgc aaggaatttt
     4261 attgaaataa ataagaacag atataaaaaa gctataaaaa cagaaaattc aaaagaaaat
     4321 gaaattaata taatcaaggt taaagacaga gcagagcagg ctaagttgat tacggatata
     4381 gttgcaaaag caaagtatga aaataagacc ataggtattt tatacagata caatatgtcg
     4441 gctatgatac cggcaaacaa tctctatgaa aataatattg atttttatat taaagaggat
     4501 aaaactaagt ttttcagcag tgctgtactt tatgatattc ttgcattttt gaatttggca
     4561 ataaacccga aggacaagga ttcatatgca aggatatatt acaaaagcta cacctatttt
     4621 accaagggta tgtgtaagat ggtactggaa agcaatgaag acaagagtgt tcttgatatt
     4681 ttgtacgggt acaagtattt tgactataac gtttatgaca aaattaagca gtttaaaacg
     4741 gatattgatt atttaaacaa gttgaagcca tccaatgcga taagatttat aaaatttgat
     4801 ttggaatatt taaattattt ggagagaatg caggaggaag gacgatacag cttatcaacc
     4861 tctcttcaca tattggagat tttggaggaa ataggctcat actgcagcga tataaaggaa
     4921 tttttaagaa aaatcaataa tttgagagat gtaataacta gagcttcaga gaacggcaat
     4981 gctcttgtaa cattgactac aatccacagt gccaagggat tggaatatga ttatgtttat
     5041 atgattgata atttaaatga attaaatatt gataaaaact tatccgttga agactatcaa
     5101 agcaaattag aggaagaacg cagaattttt tatgtgggca tgaccagagc aaaagaaaaa
     5161 ttaaatataa ttgttccagg agaaccgtct atatttgtaa atgaattgat tatatcaaat
     5221 aaatgcatgc attgattaaa cagcttcaaa aaaaatattg aaatatgctt taattaagta
     5281 tataatgtta ataattgaca agggaaaatt aacagggttt attagagggg attattcaag
     5341 ggggaaatca tgaagtttta tattagtact ctgggagaat ttgatataaa ggttgacgga
     5401 caatcaatat tgagagattc aaatcgcatg tacaggatat acaagctatt tgagtatttt
     5461 ctaacattca gaaataaaaa attattacct gaaacaatca ttgacaacct gctgtccgac
     5521 agtgaatcgg aagaccctaa gaacatgctt cgaacacaga tttttcgcct tagaaagata
     5581 ataaatgctt taattcctaa aggcgaactt ccggaaaaat atatgaattt aaactttaca
     5641 aacggatatt actgtcttga aataggtgaa aataccatag ttgatattga cgtattcgaa
     5701 aaccttatcc aaaaagggga caaggaacga gaatatgaca ttgaaagtgc aattgagagt
     5761 tatcaaaatg caatcagcct ttataaggga ctgtacttat ccgataacgc atatgaagta
     5821 tggttggtgc ctacaagaaa ttactatcaa agattatttt taaaaacatt gaataaatat
     5881 atagatttat taaaaagaaa tgaagaaaac gaaaaaattg taaatctatg cgagaaatcg
     5941 ctattgattg agccgtttga agaaagtata catattgaat tgattgaagc gcttcttaaa
     6001 atagggcaga acaaatctgc gatgcagcat tacgaatatg cattaaatat gcttgaaaag
     6061 gaattggatg caaagccttc cgctaagttc ataaactaca taaatataat acaaaactac
     6121 acttcaagaa acaatgattt ggatattgaa gcaattaata aaagcttgga ggaagagctt
     6181 ccagaaggcg caatgtattg cagtcttgag tattttaaat tcctttttaa tttacaaaag
     6241 agaaaatcca agagaaataa tgaatatgat tatcttttat caataatgat taacagcaat
     6301 aataacaaaa gtataaatga tgtattttcc gtattaaaaa aatcgttgag gaaaggtgat
     6361 gttttcacca gctggaacga taatcaaatt ttagtaatgc ttcataatgt gaaagaaaac
     6421 ggtacagaaa caattaaaga aagactgacc gacaatttaa taaattatac aagaattaac
     6481 agaaatgaaa tcagactgat ttttcaacct gtcagcatgg aaaacacgat actgtagatg
     6541 tgttggaagc aaagcaaaaa gtccgttatt gtggactttt tttattgccc ctgataaaaa
     6601 gtggtgtgtc ccagcacctt atgtaattaa attgtaatta aaaagtaatc aatattaaat
     6661 tgcataataa tagaaatgta attcatagag attataatta aaataaatat gaaggaaaaa
     6721 cgtctaagaa ggcgaggaga taaagtggag tacccttcaa aatacgattt ttatttaaaa
     6781 tttaaaccgg tatacaacaa ggaaggctat tttttgggtt atgtcttgat atatgtcagt
     6841 gattttttta ttaatgatat caatatcggg gctgatttga ccataggcaa gaaattttcc
     6901 gaaatagttg tagattcgga tattttaggt cttaaggaat tttattttaa catcataccc
     6961 aaatctaaaa tgaagcttga attatacaat gaagatttaa aaagatggta cctcataaat
     7021 gcctttacgg atatgagcaa cagggatgag catcttataa ttcattatgc ggatataaca
     7081 gagataaaac aaaaccaaca aaccgtatct ttagataata atatatatta tttaaatgac
     7141 agggaaagac ttttatataa ggataagtta accggattat acaacaaaac ctattttgag
     7201 gaagaactgt caaggcttga caccaaaaga cagcttccta taagtctgat aatgggggat
     7261 ataaacggac tcaaacttat caatgatgca tttggtcaca gcatgggtga cag
//
LOCUS       DAOPUE010000086         7285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10211_length_7285_cov_10.904149, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000086 DAOPUE010000000
VERSION     DAOPUE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10211_length_7285_cov_10.904149"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PKH42_07675"
     CDS             complement(<1..1388)
                     /locus_tag="PKH42_07675"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOA20349.1"
                     /translation="MDNRVIKSDGLAFKILEEILYIGFVLIGFYLAFLLRFNMNPSPH
                     NIQPYFDSIPYIIVATLVIFYIYNIISTIKKSLFENAVIIFISLLLINIATIAIAFFN
                     RGFAFPRSIFLIGFVIQFLLIFTTKYLIIKFIVSKRVGKDILIAVDSMEEAEYIAKKI
                     LLDKNNFDNIRYICHNINKKTYELIDTVDKIYIGDSIGNNDKLNIINYCSEKGKAIYL
                     IPSLYEISLVDFKVSQVDDLLVFKIEDLGLTYEQRVIKRILDITISSIGLIITSPLIM
                     IISIIIKLYDGGSVFFKQERVTENNKVFNLYKFRTMIEDAEKHTGPVLATEKDTRITP
                     LGRFLRASRIDELPQLFNVLKGDMSIVGPRPERPFFAEQFNEEIDGFKYRVYVKAGIT
                     GLAQILGNYSTDPKTKSKYDLLYIKNYSLLLDIKIIFNTIKIIFLKSSSKGVVNEKEL
                     DEILQSLGLNVY"
     gene            complement(1381..3237)
                     /locus_tag="PKH42_07680"
     CDS             complement(1381..3237)
                     /locus_tag="PKH42_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA20350.1"
                     /translation="MKAKTQKKKNEPITIDYENKKLSIVYFLPLLLIAGFVPLIVYAK
                     YVNLEGTKQALYWTGQRQYLDFFSYWKSMWVVILTAIALIFYIILYKQKKLPFKNLKQ
                     YYIPLGIYAFFVILSTIFAIDTQTALWGFVDMYQGMFVLISYVLITFLTINFVNSERD
                     LTLFVKAFLFLMIAEGLLGITQYFGFDFFQTKIGNSLIIPGNLQVENLSFSFGPKTIY
                     GTLFNTNFVGSFATLMLPLSIAFLLGVKEKKQRIISAIAVVLMIFVWIGCNSRAGYLG
                     VAIASIFALWLFRKVIRKYWIGFTGLVIVVVLVLVGLNHFSNNRIFSRLKVFNLKEQI
                     EITKSNANDEKKFKFEDIILGQDTFSIKTNRETLNFKIDGDKLYFLDEDNNELEITTK
                     GNEITIKDKKYAGYKIMISKKYPGITVIRNNRNFNFYFSDSGVKMLGSGGRITEPIIA
                     ETFRPLDGLEKLASNRGYIWGRTLSMLKRYVIVGSGADNYPIAFPQDDLLAKSNTFSD
                     PNTVIDKPHNLFLQIASNTGIVSLLSLLGALGIYLISGLKLYSKITFNSLEKYMGASC
                     LISIIGYLAAGMFNDSVVSVAPLFWIILGMGISINLRLKNKMFQIRDDEHNG"
     gene            complement(3449..3718)
                     /locus_tag="PKH42_07685"
     CDS             complement(3449..3718)
                     /locus_tag="PKH42_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOA20351.1"
                     /translation="MDELFLDCNKMTSKLEAHKYIKEMLNLPDYYGNNLDALWDILST
                     KNKMISIFLLHEEKLYENLGEYGKKLIDTFEEAAQSNGSIQFTRM"
     gene            complement(3730..4326)
                     /locus_tag="PKH42_07690"
     CDS             complement(3730..4326)
                     /locus_tag="PKH42_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HOA20352.1"
                     /translation="MKSIKFKILTLILAMLVIISGCVSQNNGIDKENNSSIVEDTEDI
                     VGNDTQSPVNNSNEGTGEEDRQEQEDQQDRQEPQEKQTELSIDENCTYTDPYLVAEYI
                     HKYNKLPSNYLTKNEARELGWESDKGNLWEVTDEMSIGGDMFGNREGLLPKKEGRIYY
                     ECDVNYNGGYRGAERIVYSNDGLIFYTNDHYESFTQLY"
     assembly_gap    4329..4428
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4429..6152
                     /locus_tag="PKH42_07695"
     CDS             <4429..6152
                     /locus_tag="PKH42_07695"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA20353.1"
                     /translation="FGVAPIAIQAYQALRVKVVSIDVLVTIAVTGAFLIRNFEESAIV
                     TFLFLFGAYLEQRTLNQTRSAIKELTEMAPESALKQMENGEFEEVEVDEVDVGDILLV
                     KTGAKVPVDGTVLTGEGYINEASITGESVPVGKKKDSQVYAGTILENGTIQIVADRVG
                     EDTTFGKIIELVEEAQDSKSEAERFIDRFSKYYTPAVLVLSFIVWLFSRDIELAITIL
                     VLGCPGALVIGVPVSNVAGIGNGARHGVLLKGSEVFQDFSRVDTIVFDKTGTLTIGNP
                     KVADKEIYADNVDEVLAYLASVENESDHPLAKAIVEYIGDTNVYEVQKTDVVKGGGIV
                     AEVDGHRVAVGNVKLMEQENVHINEKALADIERFEKSGNSIVLISVDGELKALIGIRD
                     QVRPGVKENLLKLKKLGVKNLVVLSGDNQGTVDLVANELGLTEAHGHMLPEDKSAFIK
                     NLIDNGHIVAFVGDGVNDSPSLALAHIGIAMGSGTDVAIETSDVVLMNSDFSRLPHAL
                     GLTKATARNMYQNIFIAVGVVLVLLASVFFSDWMNMSIGMLVHEGSILVVILNGMRLL
                     RYGARNS"
     gene            6514..6747
                     /locus_tag="PKH42_07700"
     CDS             6514..6747
                     /locus_tag="PKH42_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOA20354.1"
                     /translation="MQKATIQLETLTCPSCMLKIEGAVKSIDGVDKDSLNVSFNTSKV
                     KLNFNDEKTSIKDIENAITKLGYEVKKSTVKPL"
     gene            6813..>7285
                     /locus_tag="PKH42_07705"
     CDS             6813..>7285
                     /locus_tag="PKH42_07705"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOA20355.1"
                     /translation="MSMQGDEKMTNIYKDDAIQEGIKALAGLMKESNYTVVLTGAGMD
                     TESNIPDFRGKDGLWRKIDPRLVASVDTLEENYSMFREFYIERINRLKGVNPHEGHYI
                     LADFEEKGIIKSIATQNVSALHELAGSKNVNELHGNIRSIRCNNCNKEANIDDFI"
BASE COUNT         2377 a         1160 c         1195 g         2453 t
ORIGIN      
        1 ttatatacat ttaaaccaag agattgtaat atttcatcca attctttctc attgacaaca
       61 ccctttgagc tactctttaa aaatatgatt ttaatagtat taaaaataat ctttatatct
      121 aatagtagtg aataattttt tatatacagt aaatcatatt tagattttgt tttcgggtcg
      181 gttgagtaat ttcccagtat ttgtgctagt cctgttatac cggcttttac ataaacccta
      241 tacttaaatc catcaatttc ttcattaaat tgttcagcaa aaaatggtct ttccggtctg
      301 ggaccaacta tgctcatatc tcccttaagt acattaaata attgcggtaa ttcatcaatt
      361 cttgatgctc tcaggaatct tcccaaaggc gtgattcgtg tgtccttttc tgttgccaaa
      421 acaggaccgg tatgcttttc ggcatcttct atcatagtgc ggaatttata taaattaaat
      481 actttattat tttcagttac tctttcctgc ttaaaaaata ctgagccccc atcatacagt
      541 tttataatta ttgaaataat cataattaat ggagaggtaa taatcaatcc tatagaagat
      601 atagtaatat ctagtattct tttaatcact ctttgctcgt aagtcaatcc caagtcttct
      661 atcttaaaaa caagcaaatc atcgacttgg cttactttaa aatccactaa agaaatttca
      721 tatagactcg ggattaaata tatggctttt cctttttcag agcaataatt tataatattc
      781 aatttatcat tattacctat actgtcacca atataaattt tatctacagt atctataagt
      841 tcataagttt ttttatttat gttgtgacaa atatatctaa tattatcaaa attgttttta
      901 tctaataaaa tcttttttgc aatgtattct gcttcctcca ttgaatcaac agcaatgagt
      961 atatccttcc ctaccctttt agatacaata aattttataa ttaaatattt agtagtaaat
     1021 attaataaaa actggataac aaagcctatc aaaaatatac ttcttggaaa agcaaatccc
     1081 ctgttaaaaa aggctatagc aatagttgct atgttaatta ataataaaga aatgaaaatt
     1141 attacagcat tttcaaataa ggatttttta attgttgaaa taatattata aatatagaat
     1201 ataactaatg tagcaactat tatataaggt atactgtcaa aatagggctg tatattatga
     1261 ggggaaggat tcatattaaa tcttaataaa aaggccaaat aaaaacctat taatacaaaa
     1321 cctatatata gaatctcttc taatatttta aatgctaaac catcgctctt aattactcta
     1381 ttatccatta tgttcatcat cccttatttg aaacatcttg ttttttaatc ttaaatttat
     1441 gcttatgccc ataccaagta taatccaaaa taatggtgct acacttacta cactatcatt
     1501 gaacattcct gctgctaaat atccaataat acttattaag catgatgctc ccatatattt
     1561 ttctagagaa ttaaaagtaa ttttgctgta taattttaaa ccgctaataa gatatattcc
     1621 aagtgctccc aataatgaca aaagggatac tattcctgtg tttgaggcta tttgtaaaaa
     1681 taggttatgt ggtttatcaa tcaccgtatt cggatctgaa aaagtattag atttagccaa
     1741 taaatcatct tgcgggaatg caataggata attatcagct cctgaaccta caataacata
     1801 cctttttaac atagataatg ttctgcccca tatataaccc ctatttgatg ctaatttttc
     1861 taacccgtct aatggtctga atgtttcagc aattataggc tcagttattc taccaccgga
     1921 acctaacatt ttaactccgc tgtctgaaaa ataaaagttg aaatttctat tattccgtat
     1981 tacagtaata ccgggatact ttttagatat cattatctta tatcctgcat attttttatc
     2041 ctttatagta atttcgttac cctttgttgt tatttctaat tcattgttat cttcatcaag
     2101 aaagtataat ttatctccat ctattttaaa attcaatgtt tctctatttg ttttaattga
     2161 aaaggtatcc tgccctaata taatgtcttc aaatttaaat tttttttcat cattagcatt
     2221 agactttgtg atttcaattt gttcttttaa attaaaaacc ttaagtcttg aaaaaattct
     2281 attatttgaa aaatgattga gtcctaccaa gactaatacg acaactatta ctaatccggt
     2341 aaatccaatc caatattttc taataacctt cctaaacaac cacaatgcaa atattgaagc
     2401 tatcgcaaca cccaaatacc ctgctcttga attgcatcca atccaaacaa atatcattaa
     2461 tacaactgct atggctgata taattctttg tttcttttcc tttactccca aaaggaatgc
     2521 aatggacaga ggcaacatta gtgttgcaaa gctaccaaca aagtttgtat taaacaatgt
     2581 accgtatatt gtctttggtc cgaatgaaaa cgataagttc tcaacctgta agtttcctgg
     2641 aattataaga ctgtttccta ttttagtctg gaaaaaatca aacccgaagt attgtgttat
     2701 gcctaacaat ccctcagcaa tcataagaaa caaaaatgct ttaacaaata aagtcaaatc
     2761 cctttcgctg tttacaaaat taattgtaag gaatgtgatt aggacatagc ttattagaac
     2821 aaacataccc tggtacatat caacgaatcc ccatagggct gtttgtgtat ctatggcaaa
     2881 tatggttgat aatattacaa agaatgcgta aatacctagt ggtatataat attgctttaa
     2941 atttttaaat ggtagtttct tttgtttata cagtattata taaaaaataa gtgctattgc
     3001 tgtaagaatt actacccaca ttgatttcca gtaactgaaa aaatcaaggt actgtctttg
     3061 gcctgtccaa tatagagctt gctttgttcc ttctaagtta acatattttg catatactat
     3121 taaaggtaca aatcctgcta ttaataatag cggcaagaag tatactatgg atagcttttt
     3181 attttcataa tcaatcgtta taggttcgtt tttcttcttt tgtgtttttg ccttcacttt
     3241 attactcctg ttgaattatt atatttgatt cttcttgaga ttcttctagc taaagctctc
     3301 agaataacac ttctccagta ttcatagaaa tgacactaat cttgtcactt atagtattat
     3361 aacactaaat gtttcatttg gaaatataaa agtttcttaa cgaaacacaa ccctcactat
     3421 atgtatgagg gttgtgctta taagtttttt acatccttgt aaattgaatg cttccgtttg
     3481 attgtgctgc ttcttcaaat gtatcaatca actttttacc gtattctcct aaattttcat
     3541 atagtttttc ttcatgaaga aggaaaatcg atatcatttt gtttttggtg ctcaatatgt
     3601 cccagagggc atctaaatta tttccgtaat agtcaggcag attaagcatt tctttaatat
     3661 atttgtgtgc ttctaatttt gaagtcattt tgttgcaatc taagaataat tcatccatgt
     3721 atatgcctcc taatataatt gagtgaagct ttcataatgg tcattggtgt aaaatattaa
     3781 gccgtcgttt gaatacacta ttctctctgc accccgatag cctccattat aatttacgtc
     3841 acactcataa tatattcttc cttctttttt tggaagcaat ccttctctgt taccgaacat
     3901 gtctcctcca atactcattt catctgttac ttcccagagg tttcctttat cactttccca
     3961 gcctaattct cttgcttcat ttttagttag ataattagag ggtaatttat tatatttatg
     4021 aatgtattct gctaccaagt atgggtctgt atatgtacag ttttcgtcta ttgataattc
     4081 tgtttgcttt tcttgtggtt cctgacggtc ttgctggtct tcttgttctt ggcggtcttc
     4141 ttcgcctgta ccttcattgc tattatttac tggcgattga gtatcattac ctacaatatc
     4201 ctctgtatct tcgacaattg acgaattatt ttctttatct ataccattat tttgtgacac
     4261 acatcctgat attatcacta gcattgctag aatcagagtt aatatcttaa attttatact
     4321 tttcatttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tttggggttg
     4441 cacctattgc aattcaggct taccaagcat tgagagtaaa agttgtaagt atcgatgtat
     4501 tagttaccat cgctgttacc ggagcatttt taatcagaaa ttttgaggaa tcagccattg
     4561 ttacattctt atttctattt ggagcatatt tagagcagcg tacattgaat caaacgcgtt
     4621 cagcaatcaa ggaattaacc gaaatggcgc cggaaagtgc attgaagcaa atggagaatg
     4681 gtgaatttga agaagtggaa gtagatgaag ttgatgtagg ggatattttg cttgttaaaa
     4741 ccggtgcaaa ggttcctgtt gacggtactg tattaaccgg agaaggatat attaatgaag
     4801 caagtataac cggtgaatct gttcctgttg gtaaaaagaa ggactctcaa gtgtatgcgg
     4861 gaacaatcct tgaaaatgga accattcaaa ttgttgccga tagagtagga gaagatacta
     4921 cctttggtaa aataattgag ctcgttgaag aagcccaaga ttcaaaatct gaagccgaac
     4981 gttttattga cagattttct aaatattaca ccccggcagt cttggttctg tcctttattg
     5041 tgtggttgtt ttcaagggat attgagcttg caattacaat attggtattg ggatgcccgg
     5101 gagcattggt tataggtgta cctgtttcaa atgttgcagg tatcggtaat ggagctaggc
     5161 atggtgtttt gttaaaaggc agtgaagttt ttcaggattt cagcagggtt gatacaatag
     5221 ttttcgacaa aacgggtaca ttgacaatag gaaaccctaa agttgctgac aaagaaattt
     5281 atgcagataa tgttgatgaa gtactagctt atctggcaag tgtagaaaat gaatctgacc
     5341 atccattggc taaggcaatt gtagaatata ttggagatac taatgtctat gaagttcaaa
     5401 aaactgatgt tgtaaaaggc ggcggcattg tggccgaagt agatggacac agggttgctg
     5461 tcggtaatgt aaaactgatg gaacaagaaa atgttcatat aaacgaaaag gctcttgctg
     5521 atatagaaag atttgaaaaa agcggtaact caattgtact aatatcggtt gatggtgaat
     5581 tgaaggcact gataggtatt cgtgaccagg ttcgccccgg tgtaaaagaa aatttactaa
     5641 agcttaaaaa attaggtgtc aaaaacttgg tagttctatc gggagacaac cagggaacgg
     5701 ttgatttggt agcaaatgaa ttaggtctta cagaagctca cggacatatg cttccggaag
     5761 ataagtcggc atttatcaag aatttaatag ataatgggca tattgtggca tttgtaggag
     5821 atggagtaaa tgacagccct tcattggcat tagctcatat tggtattgct atgggaagcg
     5881 gtacagacgt agctattgaa acatcggatg ttgtattgat gaattcagat tttagccgcc
     5941 tgcctcatgc cttaggctta acaaaagcaa cggcaaggaa tatgtaccaa aatatattta
     6001 ttgcagtagg agttgtatta gtccttcttg ccagcgtatt cttcagtgat tggatgaata
     6061 tgtcaattgg aatgctggtg cacgaaggaa gtatactggt ggtaatttta aacggcatga
     6121 gactgctccg atacggtgcc agaaactcgt agaggttcag gtatcaaaaa ctagtaagta
     6181 aaaacaacat ataattttga tttaaattaa ttcaaataga aaaatattgt gtataatata
     6241 tataattcta tcgtcatcct gagtagaatg aaagatatca ggtcttgaga tttttcacta
     6301 cgttcagaat tatcacgtgt taccaaacca cgataaacga atatactctg tgattgtcat
     6361 cctgagcgca agcgaaggat ctcagggtta aaaaatgagg ttcttcgagc taaagctctc
     6421 agaatgacac gtagcttatt catagcaatg acaattaaaa aacggattag gaatgccata
     6481 taaaaataaa taaattaaaa aggagagatt aaaatgcaaa aggcaactat tcaattagaa
     6541 acattgactt gtccatcatg tatgctgaaa atagaaggtg cagtaaaatc tatagatgga
     6601 gttgataaag acagcttaaa cgtatcattc aacacaagca aggtaaaatt aaattttaac
     6661 gatgaaaaaa cttcaataaa agatattgaa aatgcaatca ctaaattggg atatgaagta
     6721 aaaaagtcta ctgtaaaacc tctgtaatac aacgtggtgc cttcccgtgg tgcctggcac
     6781 ccgaagcggt gcctggcacc attggagtat aaatgtcaat gcaaggagat gaaaaaatga
     6841 caaatattta taaggatgat gcaattcaag aaggtataaa ggctcttgcc ggcttaatga
     6901 aagaatcaaa ttatacagta gtgctcacag gagccgggat ggatacggaa agcaatatac
     6961 ctgattttag aggaaaagac ggattatgga ggaaaataga cccaagatta gtcgcaagcg
     7021 tagatactct tgaagagaat tactccatgt ttcgtgagtt ttatattgaa agaataaaca
     7081 ggttgaaagg tgtaaatcct catgaaggtc attatatact tgcagatttt gaagaaaaag
     7141 gaataattaa aagtattgca actcaaaatg tttcagcttt gcatgaatta gccggaagta
     7201 aaaatgtaaa tgagctgcat ggaaatatta ggtctataag gtgcaataat tgtaataagg
     7261 aagcaaatat agatgacttt attaa
//
LOCUS       DAOPUE010000087         7006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10723_length_7006_cov_13.802331, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000087 DAOPUE010000000
VERSION     DAOPUE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10723_length_7006_cov_13.802331"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKH42_07710"
     CDS             <1..787
                     /locus_tag="PKH42_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20356.1"
                     /translation="IVDENLKEGEFFPSSPLSYMTIKLSKLSEKAKPHEWVCGERREK
                     SNFVFSCNIYAYIKAGCPTNVEFYEKNTETGIHELLISKEFTLANPTVISVNSSIEDI
                     NGIYTGKAAGGNYTSGEKMDLYYKAIRIESNKDGILIGPCLENGDYDPEYMVQCVFNK
                     ETNRYEGKVKRESGSQWMELNFAAEINTANGKPRAKAAYSQIISSRPGSLYIYEYDVG
                     RTSSLKGSPVVRSGGTAETPAQEPTSGGQPVGGFTGPIIPEGD"
     gene            794..1240
                     /locus_tag="PKH42_07715"
     CDS             794..1240
                     /locus_tag="PKH42_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20357.1"
                     /translation="MKIKFESNSYQIIVTIAIALLLLLASGCQKSNDMPPKMEGPEPE
                     PHKGVFVSQNAIFTFDGTNRVFVEFDDEYLEALDNPPNNTYYSYVFTWYSFGDYRYDG
                     ATNLTLYHEESDTGLDFSTQGNTTTERIEISYPIPGNENIVFVKQN"
     gene            complement(1237..2127)
                     /locus_tag="PKH42_07720"
     CDS             complement(1237..2127)
                     /locus_tag="PKH42_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOA20358.1"
                     /translation="MNLQKMMSLFAAHTPSPEKFKLRSEHAYTENMSIATLIKSPDNV
                     IPAFNHSHDLYEFIIPHTPIPFLIRDNATYFGEVGWIYPVPPGLMHGMKYDTPDVSHT
                     NIVIDKKYFDGVLKEQGVNEIILNHILPISSDIKIYISVFKAEFAKVEVNRQKLRHLS
                     ALICYELIGLATVNLDLDERKDTDYYYKGIREAAEFMNNNYMNGLSIESLAKMAGLST
                     NYFSTAFLKALGDRPQIYLDKLRISRAKQLLESSNVPINEIAQMCGFKNQSSFTMLFK
                     HMTGQTPTEYRNSLKEYEDN"
     gene            2348..5752
                     /locus_tag="PKH42_07725"
     CDS             2348..5752
                     /locus_tag="PKH42_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOA20359.1"
                     /translation="MRKKTLAKVLVLIFIIISLTLTPAYAWKVKTHVYSANLILEDAA
                     DGFVELAPYGEFQVLPEYQTVLRMYPEYFRAGALGPDLMPDMLIGQTVFHPGTEYRTS
                     GEIIQTLWHLVNELPEYRPSALEQQLDSMIHSTVSIDHVPARDDYDPGDDCLEMNNKK
                     QAQAFMLGFMSHAAGDYFGHSYVNKWAQDSWPLLTDGISEADKRIIKRHSALEAYIDS
                     KIPAKYQETSYNTIASPQDFIFDNMIVNGKTDLRDFIRDHRISETFGSATASPHVELF
                     FAIRDALKQRLIDINNSSAVVKLARAPEKAYCEAWIADIDRGLGAWVKANEKAAQTML
                     NKDTTMTQYVDVLKEWKDEHLMLMLGYPDVANDVKNAIGDINDFIMGIVPARWQQKYK
                     DLKMDAINYIVKESVGIDINGWIEVFNPPISVLQTNLFENGAYETINKDMANFKTCTN
                     TDDQEFVPFKNTLTLMKLILIGKPGIDDLRRISGSDGSLSFPPYANTMTYFIRNMDIG
                     YDWEVGSPMMGFFMWGSNDDRYKILNQIFIMDPDEVKPTFSHMTQGPIEIIPGSRSWG
                     EVPDIYVKYNNTDQISNDIGLYSTESFTNYSSPEQHKTIARNENGDFTVTTPGKGGKY
                     HFRIYDKNHYLLAVSDEIEVVEVTQYSSQPAGNTQAQAVGNTQDQSVEITNTQPEQTT
                     DSNTNTTGDNNVPIGTADLSTGRIKHTWKAPGSISAAVTSEEDYSTSIFFTVDPELLN
                     FLNMSEEEENALGISSIYHTAQIDWKLNDGPWHYTQDWDTLSEYYSYYSGIYADTFYL
                     SGEIKDETTIFDLRNDSESDGLSDLQRKLGRALILGNDEYGEDNRLDLANNTFYFRVR
                     LYVYYFDESNYEHGYILTPWSETITYGKVPVSSQVTASQPPQPSSVQTSPVIDHSETF
                     DEGTVLTWPYNNPLGYRLYRSESEDELGISVTDFYVTSTSYADLNVEPNTTYYYSVKP
                     VLKEADSFTGQDEILGEVIKRYTVTTGNIANQQAGKDFIILQIDNPNMSVNGVLNEID
                     PGRGTTPMIISSRSMVPIRAVVEALNGTVGWDNSTQQITLTANGNTVIMWINKYEIIV
                     NGTIHKIDVAPTIVNGRTYVPVRFAAENLNTKVYWINTLRQAVIVN"
     gene            5801..6892
                     /locus_tag="PKH42_07730"
     CDS             5801..6892
                     /locus_tag="PKH42_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20360.1"
                     /translation="MKRRITVFIIVLMLLFSITAVDALALPEGVPSSLEAPTIKAIEL
                     KYDADGFPYFEMQVYFPQSVLNLDSESPGDGSVFWDYSEKIDKGPWSDFGGGGYIEVY
                     TESEESRVEGSPNTYKIIFRPEDEGGVDEVDIKNHIYSYKILVYYDYYEGWPDIQPIY
                     SSASNEMSIGSGSFYAHASNWAEDELNKANEAGLIPAILQGADMTKPITREEFAELSL
                     VLYEKTSGISAEPEAVNPFTDTNNPQILKALKIGVTQGTSTTTFSPKVLINREQCATM
                     LFRTIKAIAPNGDYSITGIKDFPDQADISSWAVEGTKYMSSLGIIKGDSSGKFMPKAV
                     TDAQKAINYGMATREAAILMSVRTYDVMK"
BASE COUNT         2378 a         1226 c         1416 g         1986 t
ORIGIN      
        1 aattgtggat gaaaatttaa aagaaggaga attttttcca tcttctccat tgtcttatat
       61 gacaattaaa ttatcaaagc taagtgaaaa ggcaaaaccc catgaatggg tatgcggtga
      121 aagaagagaa aaaagcaatt ttgttttcag ttgtaatata tacgcttata ttaaagcagg
      181 atgtccaaca aatgtagaat tttatgagaa aaatacagaa acaggcatcc acgagcttct
      241 aatttccaag gaattcactc tggcaaatcc aaccgttatt tctgttaaca gcagcattga
      301 agatatcaac ggcatctata caggcaaagc cgcaggagga aattatacca gcggggaaaa
      361 aatggacctc tactacaaag caatcagaat tgaatcaaac aaagacggaa ttctcatagg
      421 accctgcctg gaaaacggag actatgatcc tgaatatatg gttcaatgtg ttttcaataa
      481 agaaacaaac agatatgaag gaaaggtcaa gagagaatcc ggcagccaat ggatggagct
      541 taattttgct gctgaaatca atacagctaa cgggaaacca agagcaaagg cagcctatag
      601 ccaaatcata tcctcaaggc cgggctcctt atatatatac gagtatgatg tgggaagaac
      661 ttcttcatta aaaggcagcc cggtggtaag gtccggtggc actgctgaaa ccccggcaca
      721 ggaacccacg agcggagggc agcctgtagg cggatttaca ggaccgatta tacctgaagg
      781 agattagcat attatgaaaa ttaaatttga aagcaactca taccaaatta ttgttacaat
      841 agcaattgct ttattgcttt tgcttgcatc cggctgccaa aaaagcaatg atatgcctcc
      901 aaaaatggaa ggtccggagc ctgagcccca caaaggtgta tttgtaagcc aaaatgccat
      961 atttactttt gatggcacta atagagtatt tgtagaattt gatgatgaat acttggaagc
     1021 actggataac cctccaaaca acacatatta cagctatgtg ttcacttggt acagctttgg
     1081 cgattacaga tacgacgggg caaccaattt aaccctttat catgaagaaa gcgacacagg
     1141 cctggatttt tcaactcaag ggaataccac taccgaaaga atagaaattt catacccgat
     1201 ccccggtaat gaaaacatag tgtttgttaa acaaaactaa ttatcctcat attctttcaa
     1261 tgaatttctg tattctgtag gggtttgacc ggtcatatgc ttaaagagca tcgtaaaact
     1321 gctttgattt ttgaatccgc acatctgtgc aatttcatta atcgggacat tactgctttc
     1381 caacagctgc ttagctcttg aaatacggag cttgtccaaa tagatctgag ggcggtcccc
     1441 aagtgcttta agaaaagctg tggaaaagta gttggtagaa agtccggcca tttttgctaa
     1501 agattctatg gataaaccat tcatgtaatt gttgttcata aattctgcag cttctcgtat
     1561 tcctttatag taataatcag tatcctttct ctcatccaaa tcaagattta ctgttgcgag
     1621 acctattaat tcataacaga taagtgcaga aagatgtctt agtttttggc gatttacttc
     1681 gacctttgcg aactcagctt taaaaacact gatatatatt tttatatcac tggatatagg
     1741 taaaatatga ttgagtataa tttcatttac tccctgttcc tttaaaactc catcaaaata
     1801 tttcttgtca atgacaatat tggtatgaga aacatcaggt gtatcgtatt tcataccgtg
     1861 cattagtccc ggaggcacag gataaatcca gcccacttca ccgaaataag ttgcattatc
     1921 acgtataaga aaaggtatgg gagtatgagg aataataaac tcatataaat catggctgtg
     1981 attaaatgca ggtataacat tatccggaga ctttataaga gttgctatgg acatgttttc
     2041 agtataggca tgttcactgc gcaatttaaa tttttcaggt gaaggcgtat gtgctgcaaa
     2101 taaagacatc attttttgca aattcataat tttttccttt acttatagta ttaattaatt
     2161 atataattca tcttttcatt tttcaactga aatttaagct aatctacgat tatttttcta
     2221 tagatattat gttaaccgga aaatttgtcg tataatatat ataactaatt gtaagagcct
     2281 tgcttatgca aacattttca agcataataa atggagcaag ttgtttaaaa aaataagggg
     2341 gcataatatg cgaaaaaaaa cattagcaaa ggtgttggtt ttaatattta taataatatc
     2401 tttgactctg acaccggcat atgcatggaa ggtaaaaaca catgtgtatt ctgcaaattt
     2461 aattttggaa gatgcagcag acggatttgt cgaattagcg ccatatggtg aatttcaagt
     2521 tttacctgaa taccagacgg ttttaagaat gtatcctgag tatttcagag ctggagcatt
     2581 gggacccgac cttatgccgg acatgcttat agggcagacg gtatttcatc ccggtactga
     2641 gtatagaaca tcaggagaaa taattcaaac cctatggcat ttggttaatg aactgccgga
     2701 ataccgacca tcagcattgg aacagcaatt ggacagcatg attcattcaa ctgtttctat
     2761 tgaccatgtt cctgcccggg atgattacga ccccggtgat gactgcttag agatgaacaa
     2821 taaaaagcag gctcaggctt tcatgcttgg ctttatgtcc catgctgcag gagattattt
     2881 tggtcacagc tatgttaata agtgggcaca ggactcatgg cctttactga ctgatggtat
     2941 cagcgaagct gataaaagga ttattaaacg tcactctgca cttgaagcat acattgattc
     3001 aaaaataccg gccaagtatc aggaaacaag ctataataca attgcttcac ctcaggactt
     3061 catatttgac aatatgatag ttaatggtaa aactgacttg agagacttta tacgtgatca
     3121 tagaatatcg gaaacctttg gaagtgcaac tgcctcaccg catgttgaat tgttttttgc
     3181 catcagagat gccttaaagc aaagacttat tgatataaat aacagttcag ctgtggtgaa
     3241 acttgccagg gctccggaaa aggcatattg tgaagcctgg attgctgata ttgacagagg
     3301 ccttggtgcc tgggtaaaag caaatgagaa agcagcacag accatgctta ataaagacac
     3361 cacaatgaca caatatgtgg atgtattaaa ggaatggaaa gatgaacatt taatgcttat
     3421 gcttggttac ccggatgttg ccaacgatgt aaaaaacgca ataggggata ttaatgattt
     3481 cataatggga attgtgcctg caagatggca gcagaagtat aaagacctta agatggatgc
     3541 aataaattat attgttaaag aatctgtcgg tattgatata aatggatgga tagaagtttt
     3601 taatccacca ataagtgtat tacagactaa cctgtttgaa aacggtgcat atgaaactat
     3661 aaataaggat atggcaaatt tcaaaacctg cacaaatact gacgaccagg aatttgtacc
     3721 ctttaaaaat actctgacat tgatgaaact gattcttatc gggaaacccg gcattgatga
     3781 tttaagaagg atttccggtt cagatggatc attgtcattc cctccatatg caaatactat
     3841 gacatatttt ataagaaaca tggatatcgg gtacgactgg gaagtcggct cgccaatgat
     3901 gggattcttt atgtggggca gcaatgatga cagatataaa atattaaatc aaatattcat
     3961 aatggatcct gatgaagtaa aaccaacttt ttcccatatg acacagggac cgattgagat
     4021 tattcccgga tcaagatcat ggggtgaagt tcccgatatt tatgttaaat acaataacac
     4081 tgaccagatt agtaatgata tagggctgta ttctacggaa agctttacaa attatagtag
     4141 ccctgaacag cacaaaacta tagcaaggaa tgaaaatgga gattttacgg ttactacacc
     4201 tggaaaaggt gggaaatatc atttcaggat ttatgacaag aatcactact tgcttgcagt
     4261 aagcgatgaa atagaagtgg ttgaagtaac tcagtacagt tcacagcctg cagggaatac
     4321 tcaggcacaa gctgttggga atacccagga tcagtctgtc gaaattacta atactcagcc
     4381 tgaacagact actgacagca acactaatac caccggtgat aataacgttc ctataggaac
     4441 agcagacctg agtacaggaa gaataaaaca tacttggaag gctccgggtt ccattagcgc
     4501 agctgttaca tcagaggagg attattcaac aagtattttc ttcactgttg atcccgagtt
     4561 gttgaatttt ttaaatatgt ctgaagaaga agaaaatgca ttgggtattt ccagcattta
     4621 tcatacagca caaatcgact ggaaattgaa tgatggacca tggcattata ctcaagattg
     4681 ggatacttta agcgagtact attcatacta ttcaggcata tatgcagaca cattctacct
     4741 atccggggaa attaaagatg aaacaacgat atttgattta agaaatgact cggagtctga
     4801 tggtctttca gatcttcaga gaaagctggg aagggcatta attcttggta atgatgaata
     4861 tggagaggac aacaggctcg atttagccaa taacacattt tattttcgag taagactata
     4921 tgtctattat tttgatgaga gtaattatga acatggatat attcttacac catggtcaga
     4981 aaccattact tatggaaaag taccggtatc ttctcaggtt actgcatcac agccgccaca
     5041 gccgtcatct gtacaaacaa gtccggtaat tgatcattct gaaacatttg atgaaggaac
     5101 cgtattaaca tggccataca ataatccttt aggatacaga ttgtacagat ccgaatcaga
     5161 ggatgaatta ggaatatctg taacggattt ttatgtaaca agcacatcat atgcagacct
     5221 taatgtagaa ccaaatacaa catattacta tagcgtgaag ccggttctta aagaggcgga
     5281 ctccttcacc gggcaggatg aaatacttgg agaagtaata aaaagatata cggttacaac
     5341 cggaaacata gctaatcagc aggcggggaa agattttatc atacttcaga tagacaatcc
     5401 aaatatgagt gtaaatggtg ttttaaatga aattgaccct ggcagaggca ctactccgat
     5461 gattatatca tcccgctcaa tggttcctat aagggcagtg gtcgaagcat taaatggaac
     5521 agttggctgg gataacagca cacagcagat aactttaact gcaaacggga ataccgttat
     5581 tatgtggatt aataaatatg aaataatagt aaacggcaca atacacaaga ttgatgtggc
     5641 acctacaatt gttaacggcc gtacttatgt acctgtaaga tttgcggctg aaaatctcaa
     5701 tacgaaggta tattggataa atactcttag acaagctgta attgttaatt aaaacataat
     5761 tatagaatat tttcacaaaa ttagaaatta ggaggacaaa atgaaaagaa gaatcacagt
     5821 ttttattatt gttttaatgc ttttgttcag tatcacagca gttgatgctt tagcactgcc
     5881 ggagggcgtg ccatcatcac ttgaagcacc gactatcaaa gctatagagc ttaaatacga
     5941 tgcggatgga tttccgtatt ttgaaatgca ggtttatttt ccacaatctg tccttaatct
     6001 cgatagtgaa tcccctggcg atggttcagt tttctgggac tattcagaaa aaattgacaa
     6061 gggaccatgg agtgattttg gcggcggcgg atatatagaa gtatatactg aatccgaaga
     6121 aagtagagta gagggaagtc caaatactta caaaattata tttcgaccgg aggatgaagg
     6181 gggtgtcgac gaagtagaca tcaaaaacca tatttattct tataagattc ttgtttatta
     6241 cgattattat gaaggatggc ctgatattca acccatttat tcatccgcat ccaatgaaat
     6301 gtctataggt tccggaagct tttatgcaca tgcaagcaat tgggctgaag atgagcttaa
     6361 taaagcgaat gaagctggac ttattccggc aattttgcag ggagctgaca tgacaaagcc
     6421 tattacccgt gaagaatttg cagagctttc attggttctt tatgaaaaaa catccggaat
     6481 ttcagcagaa cctgaagcgg ttaatccttt tacagataca aataatcctc aaatattaaa
     6541 ggcattaaaa ataggtgtaa cacaaggaac atccacaaca acattttcgc caaaggtttt
     6601 aattaaccga gagcaatgtg caacaatgct ttttagaacc ataaaggcga tagctccgaa
     6661 tggggactac agtattacag gcatcaagga ttttccggac caagctgaca tttcatcatg
     6721 ggcagttgaa ggaaccaaat atatgtcaag tttaggtatc ataaaaggcg acagtagtgg
     6781 caaatttatg cccaaggctg taaccgatgc tcaaaaagca atcaattacg ggatggcaac
     6841 aagagaagca gcaattctca tgtcagtaag aacatatgat gtcatgaaat aaaactatag
     6901 atacagttaa aaagaataag cagcacctga atatcaataa cttacgatat taggcgctgc
     6961 ttattttctt tgttaatcat tctttacctc tcggtagaat tcaaca
//
LOCUS       DAOPUE010000088         6975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10788_length_6975_cov_10.176879, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000088 DAOPUE010000000
VERSION     DAOPUE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10788_length_6975_cov_10.176879"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1257)
                     /gene="rseP"
                     /locus_tag="PKH42_07735"
     CDS             complement(16..1257)
                     /gene="rseP"
                     /locus_tag="PKH42_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOA20361.1"
                     /translation="MLTLIASILVFSAIVLVHEYGHYKAAKSVGIKIYEFAIGMGPTV
                     FKKEKKGIVYSIRAVPFGGFVQMEGEEEDSRTPTSFSTKTIAQRAKVIAAGPVMNFVL
                     ALVLFLIIAFSYGVYGNHIDYIDENSNEYKAGLRTGDKIISVNGERTYIWEDIFYELV
                     SPEESSYTLEYERNGQIDKVNIDKNYRKIIGVTSTIVNGKTTTTVTPSDMTSPAYKAG
                     IMDGDIVIKINDIPVNTWDELTTQVENSNKTVKVTVDRKGEIIDYNIDLVEQISVRFN
                     TELEKSFMTAISSSMYKTVYYIELMFKTIRLLVTGKLGSDALGGPVMVISMVGEAAQN
                     GILSLLSLAAFISINLGFMNLLPFPALDGSKLVFLAIEGIRGKALPVEKEGYIHFIGF
                     VFLIGFMLFITYKDLLRVFRI"
     gene            complement(1273..2040)
                     /locus_tag="PKH42_07740"
     CDS             complement(1273..2040)
                     /locus_tag="PKH42_07740"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOA20362.1"
                     /translation="MGRRVLTGFAGGAFIALITYLGGSLYDFVFLGITCIAIYELSKV
                     FFNDGFDYGAVMNYMFAIALFIEKITENRHFFDFFLFLYISIIFLIYIFNKKVTVKKV
                     SEIIFMGTYVVFFMYHMMLMNNSKFVWLVYIIAFGTDTFAYFTGKFFGKNKLYPEVSP
                     NKTVEGAIGGIIGSTILSLIYFNYLSINKYFYIIIFSVSASVFSMAGDLTASKIKREY
                     KIKDFGNLLPGHGGIMDRFDSVLFVAPTVYYFILHFI"
     gene            complement(2051..>2761)
                     /locus_tag="PKH42_07745"
     CDS             complement(2051..>2761)
                     /locus_tag="PKH42_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOA20363.1"
                     /translation="EKALSGKIPEHIAVILDGNRRWAKQKNLPVQAGHREGALRVTDL
                     VRNCGDLGVKYLSVYAFSTENWRREKKEIDYLMNLLVEFVFKELENLHKNDVKITMMG
                     NMEDLPSKTRKEVERAIELTKNNKTLHLNIALSYGSRNEIVNAVKNIIKDYENNKIDI
                     EEINEESFKKYLFTNDMPDPDLLIRTSGELRLSNFLLYQLAYTEFYFTEVLWPDFNKN
                     ELIKAIENYQQRKRRYGN"
     assembly_gap    2762..2861
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3041..3598)
                     /gene="frr"
                     /locus_tag="PKH42_07750"
     CDS             complement(3041..3598)
                     /gene="frr"
                     /locus_tag="PKH42_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOA20364.1"
                     /translation="MYKDLIIKSEESMKKSINFLKEELATIRAGKANPKLIDKIQVSY
                     YGTMTPLNQIANISVPEPRMLLVQPWDANSLKDIERALLTSDLGINPTNDGKVIRLII
                     PVLTEERRKELLKLVKKEIENSKVAVRNIRRDTNEEIKRMEKQSEISKDDQKRAEDEM
                     QKLTDKYINLIDEIYKDKEKEVLEV"
     gene            complement(3600..4310)
                     /gene="pyrH"
                     /locus_tag="PKH42_07755"
     CDS             complement(3600..4310)
                     /gene="pyrH"
                     /locus_tag="PKH42_07755"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOA20365.1"
                     /translation="MCKYKRIVLKISGEALSGSAGHGINSEIVNKISNVIKRINEMGV
                     EVAIVVGGGNFWRGACAKDIDRTTSDYMGMLGTIINGLALQDALEKIDVETRLQTAIE
                     MRQIAEPYIRRRAVRHLEKGRVVIFSGGSGNPYFSTDTTAALRAAEINAEIILLAKNG
                     VDGVYSDDPNKNPDSVRYEELNYIDVLNKGLKIMDSTATSLCMDNKIPILVFGIETPE
                     NIEKIILGENIGTIIKEV"
     gene            complement(4374..5288)
                     /gene="tsf"
                     /locus_tag="PKH42_07760"
     CDS             complement(4374..5288)
                     /gene="tsf"
                     /locus_tag="PKH42_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOA20366.1"
                     /translation="MEITASMVKELREKTNAGMMDCKKALKETNGDIEKAIVYLREKG
                     LLQAAKKSSRIASEGLTTALISADEKKGVVLEVNSETDFVAKNEEFKSFVEETAKAVL
                     DNEPKDLEALKAVVLNDGKTVQEALTDKIAKIGENMSLRRFGEVKVDKGVVVDYIHGG
                     GKISVVVAMESEGDKEALKVLGKDIAMQVAAMSPKYISMEDVDAEFIAQEKEILTAQT
                     LNEGKPQNIVEKIVEGRLNKELKEICLLEQAFVKDADLTVKKVISDTAKKLGSDIKIT
                     GVLRYEVGEGIEKKSENFADEVAKQMGL"
     gene            complement(5356..6138)
                     /gene="rpsB"
                     /locus_tag="PKH42_07765"
     CDS             complement(5356..6138)
                     /gene="rpsB"
                     /locus_tag="PKH42_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOA20367.1"
                     /translation="MAIISMKKLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTSDKVDEAYNFIKEVVSNGDEILFVGTKKQAQESTKSEAIRCGMHYVSQRWLGGMLT
                     NYSTIRKRIDRLNELKTMEEDGTFDLLTKKEVFKLRNEAERLEKFLGGIKNMDKLPGA
                     LFVVDPRKERIAVQEAAILGIPVVAIVDTNCDPDEVDYVIPGNDDAIRAVKLLTATIA
                     DAVIEGKQGVQMTHEDTEDEVTEELEDLEELEELEEVEEELE"
     gene            complement(6319..>6975)
                     /locus_tag="PKH42_07770"
     CDS             complement(6319..>6975)
                     /locus_tag="PKH42_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HOA20368.1"
                     /translation="MMEKYFPKIVNEKFTADLENHLDDVASGIINWRDVISNFYESFK
                     KELDIAENEMKEIEIKDEVSDVKCENCGAFMVIKNGRFGKFLACPNYPECKNTKPLNG
                     KEPPEEVDETCEKCGSKMLKRKGKFGEFLACSNYPKCKNTKPIIKTIDAPCPRCGKQI
                     AIKFSKAGRRFYGCTGYPDCKYISWYEPTDKKCPECGEMMVLKNNKQTCINKKCKKSE
                     "
BASE COUNT         1909 a         1396 c          922 g         2648 t
ORIGIN      
        1 acccccgtcc ccttgtcata ttctaaatac tctcaataaa tccttataag ttataaacaa
       61 cataaaacct attaaaaaaa caaaacctat aaagtgtata taaccttctt tttcaacagg
      121 caatgcttta ccccttattc cttctattgc cagaaatacc agcttgcttc cgtctaatgc
      181 cgggaaagga agtaaattca taaagcccaa attaatgctt ataaaggctg caagactgag
      241 aagtgataaa attccgtttt gggccgcttc tccgaccata gatattacca ttacaggtcc
      301 gcctaaggca tcactgccca atttgcccgt tacaagcagt cttatggttt taaacataag
      361 ttcaatataa tatacagtct tatacattga agaagatata gccgtcataa atgatttttc
      421 aagctcagta ttaaatctta cagatatttg ctccaccaaa tctatattgt agtctataat
      481 ttctcctttt ctgtctacgg taacctttac tgttttattt gaattttcaa cctgggttgt
      541 cagctcatcc caagtgttta caggtatgtc attaattttt atgacaatat ctccgtccat
      601 aattcctgcc ttgtatgcag gagaagtcat atctgaagga gttacggttg tagtggtttt
      661 tccgtttacg atagttgagg taactcctat tattttccga tagtttttgt cgatattaac
      721 tttatcgatt tgaccatttc tttcatattc caatgtgtaa gaactttcct caggagatac
      781 taattcataa aatatatctt cccatatata tgttctttca ccgttaacgg aaataatttt
      841 atctccggtc cttaatcctg ctttgtattc attggagttt tcatctatgt aatctatatg
      901 attaccataa actccatatg aaaaagctat tattaaaaac aatacaagag ccaacacaaa
      961 gttcatcacg ggacctgctg caattacttt ggctctttga gctatggttt ttgtactaaa
     1021 gcttgtaggt gttcttgaat cttcttcttc accttccatt tgaacaaacc cgccgaacgg
     1081 aacagctcta attgaatata ctattccttt tttttctttc ttaaatacgg ttggacccat
     1141 tcctatggca aattcatata tctttatacc aacgcttttt gcagctttat aatgaccata
     1201 ttcatggaca agaacaatag cagaaaaaac aagaatagac gctattaatg ttaacaatat
     1261 agtaacctcc gtttatataa aatgcagaat aaaatagtac actgtaggtg caacaaataa
     1321 aacactgtca aatctgtcca ttatgcctcc gtgaccgggc agaaggttcc caaaatcctt
     1381 tattttgtat tctcttttaa ttttagatgc tgtcaaatcg ccggccattg aaaatacaga
     1441 tgcacttaca ctaaatatta taatgtaaaa atatttattt atgcttaaat aattaaaata
     1501 tatcaatgat aaaatcgtgc ttccgataat accgccgatt gcaccctcga cagttttatt
     1561 cgggcttacc tcaggatata atttattttt gccgaaaaat ttaccggtaa aatatgcaaa
     1621 tgtgtcagtg ccgaaagcta taatatatac caaccataca aattttgaat tattcataag
     1681 catcatatga tacatgaaga ataccacata tgtacccatg aaaattattt ctgaaacttt
     1741 ttttacagtt acttttttat tgaatatata tattaaaaat attatagata tgtataaaaa
     1801 caagaaaaaa tcaaaaaaat gtctgttttc agtaattttc tcaataaata aagcaattgc
     1861 aaacatgtaa ttcataacgg ctccataatc aaagccgtca ttaaaaaaaa cttttgataa
     1921 ttcataaata gctatgcatg ttatgcccaa aaaaacaaag tcatataagg agccgcctaa
     1981 atatgttata agtgctataa atgctccgcc tgcaaaaccg gttaacactc ttctacccat
     2041 agtacaccgc ctaatttccg tatcttcttt ttctttgctg atagttttcg attgctttaa
     2101 ttaattcatt tttattaaaa tcaggccata aaacttccgt gaaataaaat tccgtatatg
     2161 caagctgata caagagaaag ttgcttaacc ttaattctcc gctggtcctg attaataaat
     2221 cagggtctgg catatcatta gtaaataagt attttttaaa gctttcctca ttaatttctt
     2281 ctatgtcaat tttattgttt tcgtaatctt taattatatt ttttactgca ttcactatct
     2341 catttcttga gccgtagctc aatgcaatat tcagatgaag tgttttgttg tttttagtaa
     2401 gctcaattgc gcgctctacc tcttttctcg tcttgcttgg caaatcctcc atattcccca
     2461 tcattgttat ttttacatcg tttttatgaa ggttttcaag ctccttaaat acgaattcaa
     2521 ccaagagatt cattaaataa tctatttctt ttttttctct tctccaattt tcagttgaaa
     2581 aagcataaac agatagatat tttactccaa ggtcaccgca atttcttact aaatcagtaa
     2641 ctcttaaagc tccttctcta tgtcctgcct gtacgggcag gtttttttgt tttgcccatc
     2701 ttctgtttcc atctaatata actgcaatat gttcaggtat tttccctgac aacgcttttt
     2761 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagtaagata cgtacaatgt
     2881 tacggtatca tctgaatagt tttctttaat tacatatggt ttttctaagt tattgaatct
     2941 tctattactg ccttttactt tcactatatt aataataaca tttaaacttt ctaatttttg
     3001 aagtgcttct tttaatggat aaccgattaa actatccaac ttatacctct aatacttctt
     3061 tttctttatc tttatatatt tcgtctataa ggtttatgta tttgtcagta agcttctgca
     3121 tttcatcttc tgcccttttt tggtcatctt tggatatttc gctttgtttt tccatccttt
     3181 taatttcttc gttagtatct cttctgatat ttcgtactgc aacttttgaa ttctcaattt
     3241 cttttttaac cagttttaaa agttcttttc ttctttcttc cgtaagcacc ggaattatca
     3301 gccttataac ctttccgtca tttgtcgggt taattcccaa gtcggaagtt aacaaagctc
     3361 tttcaatatc ttttaatgaa tttgcatccc aaggctgaac aagaagcatt ctcggttccg
     3421 gtacggaaat attagctatt tgatttaatg gtgtcattgt accgtaataa cttacttgaa
     3481 ttttatcaat taatttcgga ttagctttgc ctgctcttat ggttgcaagc tcttccttaa
     3541 gaaaatttat tgattttttc attgattctt ctgatttaat tattaaatct ttatacatac
     3601 tatacctcct taattattgt tcctatgttt tctcccaaaa ttatcttttc aatattttca
     3661 ggagtttcta ttccaaaaac caagatagga attttattat ccatacataa tgaagttgcc
     3721 gttgagtcca ttattttcag acctttattc aagacatcta tataattaag ttcttcgtat
     3781 cttacagaat caggattttt attcgggtcg tcgctgtaaa caccatcaac accgttttta
     3841 gctaaaagga taatttctgc atttatttca gcagctctta atgcagctgt ggtatcagtg
     3901 gaaaagtatg ggttcccgga acctcccgaa aatattacaa ctcttccctt ttccaaatgt
     3961 cttactgctc ttcttcgtat gtaaggttct gcaatttgtc tcatttcaat tgctgtctgt
     4021 agtcttgttt ccacatcaat tttttctagg gcatcctgaa gagcaagtcc gtttattatt
     4081 gttccaagca ttcccatata atcagatgtt gttctgtcta tgtctttggc acaggcacct
     4141 ctccaaaagt tcccgcctcc cacaacaatt gcaacttcta ctcccatttc atttatacgc
     4201 ttaataacat tggaaatttt gttaactatt tctgaattta tgccgtgacc tgcgctgccg
     4261 gacaaggctt ctccgctgat ttttaatact attcgtttat atttgcacat tttatcctcc
     4321 tgataatttt tattttaatg gagaaccatt ggttctccat tgtgattaca ggattataaa
     4381 cccatttgtt tagctacttc atcagcaaaa ttttcagact ttttctctat tccttcgcca
     4441 acttcatatc ttaaaacacc ggttattttt atatctgagc ctaatttttt tgctgtatcg
     4501 cttattactt tctttaccgt taaatcggca tccttcacaa atgcctgctc taagagacag
     4561 atttctttaa gctctttatt tagtcttcct tcaacaattt tttcaactat gttttgcggt
     4621 ttgccttcgt ttaaagtctg agccgtaagt atttcttttt cttgtgctat gaattcagca
     4681 tctacatctt ccattgaaat atattttgga ctcattgcag caacctgcat tgctatatcc
     4741 ttgcctaaga ctttaagtgc ttctttgtca ccttcagatt ccatagcaac aacaacggaa
     4801 atttttcctc cgccgtgaat gtagtcaact acaacacctt tatcaacctt tacttctccg
     4861 aatcttctta gtgacatatt ttcaccaatt tttgctattt tatctgttaa agcttcctga
     4921 actgttttac cgtcatttag tacaacagct tttaaagctt ccaaatcttt tggttcattg
     4981 tcaagaacag ccttagctgt ttcttcaaca aagcttttga attcttcatt tttagcaacg
     5041 aaatctgttt ctgagttcac ttcaagaacg acaccctttt tctcatcagc tgaaattagg
     5101 gctgtagtaa gaccttccga tgcaattctg cttgattttt ttgcagcttg taaaagtcct
     5161 ttttccctta agtatacaat tgccttttca atatcaccgt ttgtttcttt taaagctttt
     5221 ttacagtcca tcatacctgc atttgttttt tctcttagtt cttttaccat tgaagctgta
     5281 atttccatct tatcctccat aatttaaaaa gaaggtgtaa ggaaataatt cctaacccct
     5341 tactttgttg ttttattatt ctaattcttc ttctacttct tccaattctt ctaactcttc
     5401 aagatcttct aattcttcgg ttacttcgtc ttctgtatct tcgtgagtca tctgaacgcc
     5461 ttgtttccct tcaattactg catctgctat agttgctgtc aacagtttta cggctctgat
     5521 agcatcatca ttgcccggaa taacataatc tacttcatca gggtcacaat ttgtgtcaac
     5581 tattgctact acaggaattc ctaagattgc tgcttcctga acagctattc tttcttttct
     5641 cgggtcaact acaaacaaag cacccggcag tttatccata ttttttattc cgcctaagaa
     5701 tttttcaagt ctttcggctt catttcttaa tttgaaaact tcttttttgg ttaaaaggtc
     5761 gaatgtacca tcctcttcca ttgttttcaa ttcattaagc ctgtcaattc tttttctgat
     5821 ggttgagtag ttagtaagca ttccaccaag ccatctttgg cttacataat gcattccgca
     5881 tcttatagct tcagactttg ttgattcctg agcttgtttt tttgttccga caaatagtat
     5941 ttcgtcgccg tttgatacaa cttcctttat aaagttgtaa gcttcatcta ctttatcgct
     6001 tgttttctgt aaatcgataa tatagattcc atttctttcc gtgaaaatat actctgccat
     6061 tttaggattc catcttcttg tttgatgtcc aaaatgaacc ccggcctcga gcaatttttt
     6121 catactgata attgccatta tttttttcct ccttttgttt tacctccaga aatgtcatat
     6181 catgaaaaag accgtttccg gcacatctct aacatgatca aaatctgtgt gtaattatga
     6241 ttagtatatc atagccaatt ttatttggca atatttaatt acaaaaatgt gcatattata
     6301 agctattttt caataatatt attcgctttt tttacatttt ttatttatgc atgtttgctt
     6361 attgtttttt aaaaccatca tttcgccgca ttcgggacat tttttgtcgg tcggctcata
     6421 ccatgaaata tatttacaat caggataacc tgtgcatccg taaaatctcc gtcctgcttt
     6481 tgaaaacttg attgcaattt gcttgccgca tcgcggacag ggagcatcaa tagttttaat
     6541 aatcggtttt gtatttttac atttgggata attggagcat gccaaaaatt ctccgaactt
     6601 tcctttcctc ttaagcattt tagagccgca tttttcgcat gtttcatcta cttcctcagg
     6661 cggttctttg ccgtttaggg gctttgtatt cttacattcg ggataattgg gacatgcaag
     6721 aaactttccg aatcttccgt ttttaataac catgaatgcg ccgcagttct cacatttaac
     6781 atcacttact tcatctttga tttcaatttc tttcatttca ttttccgcta tatcaagctc
     6841 ttttttaaag ctttcgtaaa aattcgaaat tacatcgcgc caattaataa ttccgctagc
     6901 aacatcatct aagtggtttt ctaaatctgc ggtaaatttt tcattcacaa ttttcgggaa
     6961 gtatttctcc atcat
//
LOCUS       DAOPUE010000089         6968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_10803_length_6968_cov_11.617388, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000089 DAOPUE010000000
VERSION     DAOPUE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10803_length_6968_cov_11.617388"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..776
                     /locus_tag="PKH42_07775"
     CDS             216..776
                     /locus_tag="PKH42_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20369.1"
                     /translation="MKNKIILILICFLLFITGCAVNDNPPEKETEPYGKYTANGRIVK
                     IDKDGFHVEGEDNVGLYKVDMEKNKNFYIGERVRLNSMDGNSYDVELDEEYDYTAAMT
                     AEIFDDDSKLDLKVGEISRDEMGRMRIYGLAADDKEYDIIVGADTITNFAHSTLKVGD
                     NIYVYPKDVPNGIPAVVDAKAVTVVR"
     gene            908..2425
                     /gene="hutH"
                     /locus_tag="PKH42_07780"
     CDS             908..2425
                     /gene="hutH"
                     /locus_tag="PKH42_07780"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOA20370.1"
                     /translation="MDKVIITGNDLTLDEVVAVCRNYKKVELSDFAVERILKSRKVVD
                     DFVENEDVVYGITTGFGKFSDVTISKEESKTLQKNLIISHSVGAGKPFDSEVVRGIIL
                     LRINNLAKGYSGARLETIQTMIEMLNRKVHPIIPEKGSLGASGDLAPLSHMVLPMIGL
                     GQAEYEGEIMAGEEAMEKAGIPVIELTAKEGLALINGTQVMTALAALTVYDSLNIIKT
                     ADIAAALSFEAQNGIIDALDQRLHDIRPHKGQIDTARVLTELLTGSKMTTKQGEIRVQ
                     DAYSLRCVPQVHGASKDAINYVKEKIEIEINSVTDNPIIFPETNEGISGGNFHGQPMA
                     LSLDFLGIALSELANISERRIERLVNPTLSGLPAFLVEHGGLNSGFMIVQYSAAALVS
                     ENKILAHPASVDSIPSSANQEDHVSMGTIAARKAKEIMENVRRVLAMEIMCACQGIDL
                     RGNKGLGAGTKPVYDEVRKVVPMLKEDRPIYDDINKCEELIKNGTITKACEGRIR"
     gene            2555..3136
                     /locus_tag="PKH42_07785"
     CDS             2555..3136
                     /locus_tag="PKH42_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20371.1"
                     /translation="MNNNERWLVEESRKGNVEAFEELIKDYKKTAYNIALRIMRNVED
                     AEDASQEALIKIFKNISSFNMESTFKVWMYRIIVNTCIDFKRRKNISTVSIDETMDLG
                     SGREVQREISDESYNPDALIERNYSTQLVNDAINALEDDFKTIIILRDIKGFTYDEIS
                     QILSCNLGTVKSRLSRARKRLRELLENEMKVQG"
     gene            3149..4420
                     /locus_tag="PKH42_07790"
     CDS             3149..4420
                     /locus_tag="PKH42_07790"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOA20372.1"
                     /translation="MKCNEINDKLSLYIDNELSHEEMQQVEEHLHSCEKCQKILEEYK
                     NLISVLRNLPEEEPPVGYCKRLHEKLLESNPQSKTKKAASKIKSFNRNKWLKYGSLAA
                     ALVLVFVAANINNIGRMGMKAKNESYDMATTESPAEYYGAGSPAPEAPQAGPPVMTEE
                     AAEEGYDGDNKSARDDLSYRTNDSVMMTSMEQREMKIIKTGTISAQTEDYDMFLNDLS
                     MKVSSMDGFLESNNTQVYQVYNEKKLMYGSIRIRVPQESFYELIDYLENTVDIKRKNI
                     NETDVTKEYYEKDNKVKNLEVQEQHLRELFEKATTVEEMLLIENELRRIRTEIDALNI
                     SLSDINDRAKMSTINLEIEEVMTASLTLKTKDSVWDRSKEGFINTVNGIIRTVENIIV
                     SIVSSSPILIPAIIICIVGFLKIRKYWHHKI"
     gene            4724..5695
                     /locus_tag="PKH42_07795"
     CDS             4724..5695
                     /locus_tag="PKH42_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA20373.1"
                     /translation="MIKTNKTIAELKPGEKADGFYLIKSYEVKKTTNGKQYIDIELVD
                     KTGEINAKVWDYSKETEILIEKNPIVKVRGEVLEWSGTRQLKVNKIREKKAEDNVQIQ
                     ELIPAAPIEPSEMLEKVKSYINKIKDNEIRTLTDNIFKKYENKLYYYPAAKRNHHSYR
                     GGLLYHNLRMLQSGEKLCQVYDFLNTDYVYAGIILHDMCKILEMESDEYGIVSDYSME
                     GKLLGHIIQGVKEIEFEGEKIGIDREKSIILQHMVLSHHFEPEFGSPKKPMTPEAELL
                     HFLDIMDARMFDFEHFLEMVEPGQFTDKIWLLDNRNLYKLKGDNKDS"
     gene            complement(5720..6400)
                     /locus_tag="PKH42_07800"
     CDS             complement(5720..6400)
                     /locus_tag="PKH42_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20374.1"
                     /translation="MASGILKDPAKMENLIGMIRTFAPLTSPQTVSKVNTYLPAVEKA
                     STLLGMYSFISKAQNFRPIQAIDAKTPADKVTALLKNGNIPVTKLMAQPLIAENMQKI
                     VSALAMNMAKNGGLNEMLSSLTNSNNNNLSDMLSSFSDGNKENTDLSNLIEKFMPMIN
                     NVMSSSNKTEEPNKPYEEEVFKKAQFDHKKNDEINNKTINTPPSAEKNIRSEKHIQKP
                     IRIRQRRR"
     gene            6643..6951
                     /locus_tag="PKH42_07805"
     CDS             6643..6951
                     /locus_tag="PKH42_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20375.1"
                     /translation="MSNIFRSDNEINKIKDSKEFKDAEAKANELAKNKQFQEFKKKYE
                     GKSEEDILRDARNMSKKLKQQYGEEEYNKKIEDLKNFEKFLTPEQKRKMRKFLENLED
                     "
BASE COUNT         2566 a          975 c         1455 g         1972 t
ORIGIN      
        1 tagcacaaaa aggtacaaca tgtaaataat tatttaataa atttttaaat aatgcatatg
       61 aatttagaat tcgaggcgag ccgggaattc atgatttaag cctttatgaa tctttaaact
      121 ataaaaagta ttatcgctac aattaatatt gcatgaatat atagtattat tttggcaaat
      181 ataaataata cataaatgtt aaattggagg tatttatgaa aaataaaata atattaatat
      241 taatttgttt tttgctgttc ataacaggat gtgccgtaaa tgataatcca ccggaaaaag
      301 agaccgagcc atatggtaaa tatactgcca atggaagaat tgttaaaata gataaagacg
      361 gatttcatgt tgaaggtgaa gataatgtgg gactttacaa agttgatatg gagaaaaaca
      421 aaaatttcta cataggtgaa cgcgtaagat taaattctat ggatggaaac agttatgatg
      481 ttgaattaga cgaagaatat gattacactg ccgcaatgac tgcagagatt tttgatgatg
      541 attctaaatt ggatcttaaa gttggcgaga tatcgagaga tgaaatggga agaatgagga
      601 tttacggact ggcagctgac gataaggaat atgatataat agtgggcgcc gatacaataa
      661 caaattttgc tcattcaaca ttaaaagtag gcgataatat atatgtatat cctaaggatg
      721 taccgaacgg tatacctgca gtagttgacg caaaagctgt tacagtggta cgttaatata
      781 atttctgcac tgattgacaa aataaataat gtgttataga ataaaaatac ttcaagaaaa
      841 tggatgttag gaggtaagtt ttagcgtttt attatgctga aatttacctt tttttaaggg
      901 aggaaaaatg gataaggtta taattacagg aaatgatttg acattggacg aagtagtagc
      961 agtgtgcaga aattataaaa aggtagaatt atcagatttt gctgttgaaa ggattttgaa
     1021 atcaagaaaa gttgttgatg actttgttga aaatgaagat gtagtctacg gtattacaac
     1081 aggcttcgga aagttcagcg atgtaaccat atcaaaagaa gaatcaaaaa cactccagaa
     1141 aaatttaata atatctcact cggtaggtgc aggaaaaccg tttgacagtg aggttgtcag
     1201 aggaataata ctgttgagaa taaacaatct ggcaaagggt tattcaggtg cgaggcttga
     1261 aacaatacag acaatgattg aaatgctgaa cagaaaagtt catccaatca tacctgaaaa
     1321 aggctctctc ggtgcaagcg gggatttagc tcctctgtca cacatggtgc tgcctatgat
     1381 agggcttggg caggcggaat acgaaggaga aataatggca ggcgaagaag ctatggaaaa
     1441 agcgggaata cccgtaattg agctgacagc caaggaagga cttgctctta taaacgggac
     1501 acaggttatg actgccctgg cagcattgac tgtttacgat tcgttaaaca tcataaaaac
     1561 agcggatatt gcagcagcgt taagctttga agctcaaaac ggaataatcg atgcattgga
     1621 ccaaaggctc catgatattc gtcctcacaa gggtcaaata gatactgcaa gagtattgac
     1681 tgagctgttg acagggagca aaatgacaac caagcaaggg gaaattcgtg tacaggatgc
     1741 ttattccttg cgatgcgttc ctcaggtgca cggggcaagc aaggatgcaa taaattatgt
     1801 taaggagaaa atagaaatag aaataaattc agttaccgat aaccccataa tattccctga
     1861 aacaaatgaa ggtatttcgg gaggaaattt ccacggacag cctatggcac tctccttaga
     1921 ctttttagga attgcacttt ctgagcttgc aaatatttcc gaaagaagaa ttgaaaggct
     1981 ggtaaatcct acacttagcg gattacccgc atttttggta gagcatggcg gattaaattc
     2041 ggggtttatg attgttcaat attctgcagc tgccttagtt tccgaaaata aaatattggc
     2101 acatcctgct tctgttgaca gcattccatc ctctgcaaat caagaagacc atgtttctat
     2161 gggaacaatt gctgcaagaa aagcaaaaga aattatggaa aatgtgcgaa gagtcttggc
     2221 tatggaaata atgtgcgctt gtcagggaat agacctaaga gggaacaaag gattaggagc
     2281 gggaacaaaa ccggtttacg atgaggtaag aaaagttgtt cccatgctaa aagaagacag
     2341 accaatttat gacgatatta ataaatgcga agaattaatt aaaaacggga caattacaaa
     2401 ggcttgcgaa ggcagaataa gatagatgct aatcatcctg ctgccttttt attgattata
     2461 attaacttga atgttataat taaacaaaat caaaaattcg tggaacaaat taaaaaatgt
     2521 ttacgtctaa tatataaccg aacggggtgg aataatgaat aataatgaaa ggtggcttgt
     2581 agaggaaagt cggaaaggta atgttgaagc ttttgaagag cttatcaagg attataagaa
     2641 aactgcctac aacattgctc ttcgcataat gagaaatgtt gaagacgcag aagatgcttc
     2701 tcaggaagca ctgataaaaa tatttaaaaa tataagcagc tttaatatgg aatctacatt
     2761 taaagtatgg atgtatagaa ttatagtaaa cacatgtata gatttcaaac gcagaaaaaa
     2821 tataagcaca gtatctatag atgaaacaat ggatttaggc tccggaagag aagttcaaag
     2881 agaaatttca gatgaatcct ataacccgga tgctttaatc gaaagaaatt acagtaccca
     2941 gcttgtaaac gatgcaatca atgcgttgga ggatgatttt aaaacaataa taatactgag
     3001 ggatataaaa ggattcacat atgatgaaat atcacaaata ttatcctgta atttaggcac
     3061 ggtaaagtca agattgtcaa gagccagaaa aagattaaga gaactattgg aaaatgaaat
     3121 gaaagttcag ggctaaggtg gtgataaaat gaaatgtaac gaaataaatg acaaattgtc
     3181 attatatatt gataatgaat taagccatga agaaatgcag caagtggaag agcatttgca
     3241 ttcatgcgaa aaatgtcaaa aaatcttgga agagtataaa aatttaatat cagtattgcg
     3301 aaatttgccg gaagaagagc cacctgtagg ttactgcaaa agacttcatg aaaaattatt
     3361 ggagagcaac cctcagtcaa aaacaaaaaa ggcagcttca aagataaaaa gtttcaatcg
     3421 caataaatgg ctgaaatacg gcagtcttgc cgctgcactg gtattggtgt ttgtagcggc
     3481 caatataaac aatataggca gaatgggaat gaaagctaaa aatgaatcct atgatatggc
     3541 aacaactgaa tcaccagccg aatactacgg tgccggctcg cctgccccag aagcacccca
     3601 agcaggaccg ccggtaatga ctgaagaagc tgcggaagag gggtatgacg gtgataataa
     3661 gagcgcaaga gacgaccttt catatcggac aaatgattct gtaatgatga cttcaatgga
     3721 acaaagagaa atgaaaataa tcaagaccgg aacaatttca gcacaaacag aagattatga
     3781 catgttttta aacgatttgt caatgaaagt aagttccatg gacggattct tggaaagcaa
     3841 taatactcag gtttaccaag tatataatga gaaaaaatta atgtatggga gcattagaat
     3901 aagagttcct caggaaagtt tttatgaatt gatcgattat ctggaaaata ccgttgatat
     3961 aaaaagaaag aatatcaatg aaacagatgt aaccaaggag tattatgaaa aggacaataa
     4021 agtaaaaaac ttggaagttc aggagcagca cttaagagaa ttgtttgaaa aggcaacaac
     4081 ggttgaagaa atgcttctca tagaaaatga gcttagaaga atcagaactg aaatagatgc
     4141 tttaaatatt agtttaagcg atataaatga cagggctaaa atgtcaacta ttaatttgga
     4201 aattgaagaa gtgatgacag caagtcttac cttaaaaact aaggacagtg tgtgggacag
     4261 atcgaaggaa ggttttatta atacagttaa cgggattata aggactgttg aaaatataat
     4321 tgtaagcata gtatcaagtt cacccatact aattcctgca ataatcattt gtatagttgg
     4381 cttccttaaa ataagaaagt attggcatca taaaatttaa cagtttgaca tcctgtgcag
     4441 ttgagacatt tctcatgcat tgaagacaca ccttttattc aggtgtgttt ttgttttaca
     4501 aaattctgtt tatagatttg aataagtatg gtataatgat taaaatcaat gaagcaaaga
     4561 ctttggtctt tgccctaatg cattcattgt atcggtgtga agcaagtcca ttttgcggct
     4621 tgcacctatg atacaattaa tcatcaaatt aaaagtgagg aaaattatga gccaaagtat
     4681 tgctgatatg cttagtgatg aatcaagaga acagatgaaa agtatgatta aaacaaataa
     4741 aacaattgcc gagcttaagc ccggagaaaa ggcggacggg ttctatttga ttaagagtta
     4801 tgaagttaaa aaaactacta acggtaaaca atatatagat attgagctgg ttgacaagac
     4861 aggtgaaata aatgcaaagg tgtgggatta ctctaaagaa acggaaatat taattgagaa
     4921 aaatcccatt gtaaaagtaa gaggagaggt cttggaatgg agtggaacca ggcagcttaa
     4981 agttaataaa ataagagaga agaaggctga ggataatgtg cagattcagg aattaatacc
     5041 tgctgcaccg attgagccga gcgaaatgct tgaaaaagta aaatcctata taaataaaat
     5101 aaaggataat gaaattagaa ccttaacgga taatatcttt aagaaatatg agaataaatt
     5161 gtattattac cctgcggcaa aaagaaatca ccattcatac aggggaggac ttttgtatca
     5221 taatcttcga atgcttcagt caggcgagaa gctttgtcag gtatatgatt ttcttaatac
     5281 ggattatgta tatgcaggca taattcttca tgatatgtgc aagatattgg aaatggaatc
     5341 cgatgaatac ggaatagtat ctgattacag catggaaggt aaattgctgg gacatataat
     5401 tcaaggggta aaggaaattg aatttgaagg tgaaaagata ggtatagaca gagaaaaaag
     5461 cattattttg cagcatatgg ttttatcaca tcattttgag ccggagtttg gcagtcctaa
     5521 gaaacccatg acaccggaag cagagctttt gcatttcttg gatataatgg atgcaagaat
     5581 gtttgatttt gaacactttc ttgaaatggt cgagccggga caatttaccg ataaaatatg
     5641 gcttttggat aacagaaatc tttataaatt aaaaggagac aataaagatt cttaacccct
     5701 taggattaag aatctttttt tatcttcttc tttgtcttat gcgaattggt ttttgtatat
     5761 gtttttcact ccttatgttt ttttctgctg aagggggcgt gttaatagtt ttattattta
     5821 tttcatcgtt tttcttgtgg tcaaattgag ctttcttaaa tacttcttct tcataaggtt
     5881 tatttggctc ttctgtttta tttgaagaag acatcacatt gtttatcatc ggcataaatt
     5941 tttctattaa attgcttaaa tcagtatttt ccttgttgcc atccgaaaaa gaagaaagca
     6001 tatcacttag gttattgtta ttgctgtttg ttaaagaaga aagcatttcg tttaaaccgc
     6061 cgtttttagc catattcatc gccaaggcac ttactatttt ttgcatattt tcggctataa
     6121 gtggttgtgc cattaacttt gtaacaggaa tattgccgtt tttcaacaat gctgttactt
     6181 tatccgccgg tgttttggcg tctatagctt ggattggcct aaaattctga gccttgctga
     6241 taaatgagta catgccgagc aaagtgcttg ctttctctac tgccggcaga tatgtattta
     6301 cttttgatac tgtttgcggt gaagtaaggg gagcaaaggt tcgaatcatg cctattaagt
     6361 tttccatctt agccgggtcc ttgagtatcc ccgaggccat cgttttagtt gttcccgaaa
     6421 gcttggaaac aacaccgctt aattgattgc cgtcaaaaat atattttgcc gctaatatat
     6481 ataataatat ttgaaccgga ataatatcac cttcttataa atatctccat atatcatatt
     6541 ccgattgcat gattatgtta cagagcaaac tgtaattgta taaacactgt tgtaattaaa
     6601 tgacagcggc ggcatattat ataacatata ggaggtagat gaatgtcaaa tatatttaga
     6661 agtgacaatg aaattaataa aatcaaagat agcaaagaat ttaaggatgc tgaagctaaa
     6721 gcaaatgaat tagcaaaaaa taaacaattt caagaattta aaaaaaagta tgaagggaaa
     6781 tcggaagaag atattttacg ggatgcaaga aatatgagca aaaagttaaa acagcaatat
     6841 ggtgaagagg aatataacaa aaaaattgaa gatttaaaaa atttcgagaa attcctgaca
     6901 ccggagcaga aaagaaaaat gagaaagttt ttagaaaact tggaagatta gggagcaaaa
     6961 gctccctt
//
LOCUS       DAOPUE010000090         6735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11298_length_6735_cov_10.429192, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000090 DAOPUE010000000
VERSION     DAOPUE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_6735_cov_10.429192"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1239
                     /gene="murG"
                     /locus_tag="PKH42_07810"
     CDS             124..1239
                     /gene="murG"
                     /locus_tag="PKH42_07810"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOA20376.1"
                     /translation="MRVLITGGGTGGHINPALAIAQKVKNENPSDLVLYVGSKNSLES
                     ELVPKEGFEFKAVTAKYFKRKFTLDNIKTLFASAKGIIESVKIINEFKPNIVVGTGGY
                     VSGPVVFAAHLKRIPTMILEQNVFPGITNRLLSRFADVVGISFEEAKKYFPQNAQEKL
                     IMVGNPVRKDIITIDRRKSRTRYNLKPDDVFIYSFGGSGGQISLNEAIIDVIKKYNGK
                     QNVKILHVTGKMHYDAFMEELNRNIKLEKNIEIKDYMYEAPSALSASDVVIGSAGAIT
                     IAEITAIGVPSILIPKTYTAENHQEYNARALEKEGAALVILEKDLNGRTLLKSIEEVI
                     ENKSRLNTMALNSKRLGNFDVENKIYATMMALINAHK"
     gene            1299..2558
                     /gene="murA"
                     /locus_tag="PKH42_07815"
     CDS             1299..2558
                     /gene="murA"
                     /locus_tag="PKH42_07815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOA20377.1"
                     /translation="MGSFIVEGRNSLEGIVDIGGSKNAALPILASTVITGREYILENI
                     PDIEDVKDMLEILSNMGCSVQFENGIALIDTRGLNTHIVPEKLVRKIRSSIILMGAVL
                     QRTGKVEVAYPGGCEIGLRPIDLHLKGLRKLGVEITEKNGLLVGSVNEALSCDIHLDY
                     PSVGATENIMLFAVGNPGKTTIYNPAREPEITDLQNFLNTCGFCVSGAGTGIITIEGK
                     KVQNTDTVEYKIISDRIEAGTYLSAAASMKSKIYIRNIEKSHFKSITSLYEDAGCEIR
                     ETHGTIVIANNKRIKAIEKITTQPYPGFPTDMQAQVMASMTVADGTSIIEETVFDSRL
                     KHVPELQKMGANITTIGRVAVVEGVEKLYSAEVEAKDLRGGAALVIAAMVAEGTSKIN
                     NIHHIKRGYEKFIYKLQNLGVIITEIE"
     gene            2772..3554
                     /locus_tag="PKH42_07820"
     CDS             2772..3554
                     /locus_tag="PKH42_07820"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOA20378.1"
                     /translation="MDENNNKKTQRKKKRRRNKKLMVSTAAFITAAVVVVFCICYVYY
                     KTPYFNLIGVDIDGNLTYSDEYIMEKSGIEVGKKIFEVDRSKVKENIENEVYVENARV
                     VYELPNKIFLDIKERVNTYQVFFNNEYIIIDKNGIVLRTCNEKLDLLTIESYTDVVYN
                     IGDNIQFAGVENNEKVFNAIEYLNNEYGSDIIKAFNIELNNSFLFKTQYGTAVRIDLS
                     EDINYQIVFGMKIINERLNNNLTVATGLIDFTKGDSPVYIED"
     gene            3565..4281
                     /locus_tag="PKH42_07825"
     CDS             3565..4281
                     /locus_tag="PKH42_07825"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOA20379.1"
                     /translation="MKNKIWQKVMMFSISLILGIILVTQMRTKSNILGTVNLDKKAVE
                     LNEELAKLSIEKNRLRDELESLKKESRESEELFKDAEEELLKLKEELKVQKIISGYYD
                     VKGPGTIITIDAEPDTYLSLASSHQYILALISYLNNAGVEAISINGQRYTNYTEIVPV
                     LEHINVNGVAMVLPLEIKAIGDRSTINSSLNFVGGIVSQLNEIGYIIETEDSDEIFIP
                     RYDGEKQFKYALPIIRDEEI"
     gene            4285..5016
                     /locus_tag="PKH42_07830"
     CDS             4285..5016
                     /locus_tag="PKH42_07830"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOA20380.1"
                     /translation="MSTIKKTLNIIIFVLVFLTAAAIVNGADAGIDEYTIYTIRDLNK
                     YAHSIKEMKLLVNNMRNAVESEKRTIADYESKKYLDDIIEIKSQIEQIKLYNGFTAVR
                     GPGIMLRVSDNINEDYDLELMERIVHDVDIVVLLNDLKAAGAEAIEVNGKRIINISEV
                     VCAGPLIRVNGEVIPAPFVIIAIGDMDELYDAVTEEGTYAYELKKTYGMEVVVAQGYN
                     LTVREFRGINYETKYAEIVEEGEKK"
     gene            5013..5357
                     /locus_tag="PKH42_07835"
     CDS             5013..5357
                     /locus_tag="PKH42_07835"
                     /inference="COORDINATES: protein motif:HMM:NF018632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HOA20381.1"
                     /translation="MIYVAIGLILGIVAGLNLNFIYNPDYIVYISLAILAILNTIINI
                     LHENKTSELTITKSIAFLLTDLLFALFLGFVGEQLGLPIYLAAVFAFGNNIYKKIKVI
                     VDIYLEKYFINK"
     gene            5493..6542
                     /gene="ftsZ"
                     /locus_tag="PKH42_07840"
     CDS             5493..6542
                     /gene="ftsZ"
                     /locus_tag="PKH42_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOA20382.1"
                     /translation="MFQFEEPSERLANIKVIGVGGGGNNAVNRMIDAGVRGVNFISIN
                     TDKQALGLSKAETKFQIGEKLTNGLGAGSKPEIGEKAAEENKQDIAELVEGADMVFIT
                     AGMGGGTGTGAAPIIANIAKSMGILTVAVVTKPFMFEGRCRMRNAEMGLKRLKDHIDT
                     LVVIPNDKLLEITDKKTTMLEAFAMADEVLRQGIQGISDLIAIPAIMNLDFADVETIM
                     SGQGIAHMGIGMASGENRALNAVKQAIQSPLLETSINGARGVLVNITGGPSMGIHEVN
                     EAAKLIEEAADPEANLIYGANIDESAGDTIKITVIATGFADGAKKSDGEPSEKSEKKP
                     ENPFDFTIPSFLNKK"
BASE COUNT         2540 a          933 c         1419 g         1843 t
ORIGIN      
        1 agattcttcg agctaaagct ctcagaatga cataaatatt attcatagca atgacaagta
       61 ataaacggtt attcaaagtg tgacacatgt tgcggtaatt taataaaagg aatggtaata
      121 taaatgagag ttttaataac aggcggaggt accggaggtc acataaatcc ggcattggcg
      181 atagcacaga aagtaaaaaa cgagaatcct tctgatcttg ttttgtatgt gggaagcaaa
      241 aacagtcttg aaagcgagct ggttccaaag gaaggctttg aatttaaagc tgttacggcc
      301 aaatatttca aaagaaaatt tacccttgat aatatcaaga ccttatttgc ttcagcaaaa
      361 ggcataattg agtctgttaa aataataaat gaatttaaac ccaatatagt tgtaggtacg
      421 ggaggatatg tgagcggtcc cgttgttttt gcagctcatt taaaaagaat tcctacaatg
      481 atattggaac aaaatgtttt tcccggaata accaacagat tattaagcag atttgccgat
      541 gtggtgggaa taagctttga agaagccaaa aaatattttc cccaaaatgc gcaagaaaag
      601 cttatcatgg tgggaaatcc ggtaagaaag gacataataa caatagacag gagaaaatca
      661 agaaccaggt acaatttaaa accggatgat gtttttattt attcgtttgg cggcagcgga
      721 ggacaaataa gtttaaacga agcaataatt gatgttataa aaaaatacaa tggcaagcaa
      781 aatgtaaaaa tactccatgt aacgggaaaa atgcattacg atgcattcat ggaagagtta
      841 aacaggaata ttaaattaga aaaaaatatt gaaataaaag attatatgta tgaagctcca
      901 agtgcccttt ctgcatctga tgttgttata ggaagtgcag gagcgataac aattgcggaa
      961 ataaccgcaa taggagtacc gtcaattctg attccgaaaa catataccgc agaaaatcat
     1021 caggaatata atgcccgtgc tttggaaaag gaaggtgcgg cattggttat attggaaaaa
     1081 gacttaaacg gcagaactct tttaaagtca attgaagaag ttattgaaaa taagagcagg
     1141 ttaaacacta tggcgcttaa ttccaaaaga ctcggaaatt ttgatgttga aaataaaatt
     1201 tacgcaacaa tgatggctct cattaatgca cataagtaac aagtgaaaaa tctgtgcata
     1261 acatatatta tgaatcagct tgttgcggag ggataaaaat gggaagcttt attgttgaag
     1321 gaagaaacag tcttgaaggt attgtcgata tcggaggctc gaaaaatgct gctcttccta
     1381 ttctggcaag cactgtaatc accggtagag aatatattct tgaaaatata cctgatattg
     1441 aagatgtaaa agacatgctt gaaatattaa gtaatatggg atgcagtgtc caatttgaaa
     1501 atggtattgc gttaattgat acaagaggtt tgaatactca tatagtacct gaaaaattag
     1561 taaggaaaat aaggtcctca ataattttaa tgggggctgt tttgcagcgc acggggaaag
     1621 tggaagttgc atatcccgga ggttgtgaaa taggtcttag accaattgat ttgcatttaa
     1681 agggattaag aaagcttgga gttgaaataa ctgaaaaaaa tggtttgctg gtcggctcgg
     1741 taaatgaggc gcttagttgt gatattcatc ttgactatcc gagtgtcggg gcaactgaaa
     1801 atattatgct ttttgccgtc ggaaaccccg gaaagactac tatttacaat cctgccaggg
     1861 agcctgaaat tacagacttg caaaattttt tgaatacttg tggtttttgt gtaagtggtg
     1921 caggaacagg tattattact atagaaggaa aaaaagttca aaacacagat actgtagaat
     1981 acaaaattat ttccgaccgt attgaggcag gaacttattt gagtgcagca gcatcgatga
     2041 agagcaaaat ttacataaga aacatagaaa aatctcattt taagtctatt acttctttat
     2101 atgaagatgc gggatgcgaa ataagagaaa cacacggaac aatagttatt gcaaataaca
     2161 aaaggataaa agcaatagaa aaaataacaa ctcaacccta tccgggtttt cctaccgata
     2221 tgcaggcaca ggttatggca tccatgaccg tagctgacgg aacttcaatt atagaagaaa
     2281 cagtatttga cagcaggcta aaacatgttc ccgagctgca aaaaatggga gcaaatatta
     2341 caactatcgg gagagtagca gttgtggaag gagttgaaaa actttacagc gccgaagttg
     2401 aagccaaaga tttaagagga ggtgctgcac ttgtaattgc agctatggtg gcagaaggca
     2461 catccaagat taataatatc catcatataa aaagaggata tgaaaaattt atatataaat
     2521 tacaaaactt aggagtaata attaccgaaa ttgaatagtg taagataaat ttaaaaattg
     2581 tcatcttaat agtaagcgaa ggatatctga aatatgtcat cctgagcgta agcgaaggat
     2641 ttctgaagta tgtcatcctg agcgtaagcg aaggatctca tgagattctt cgagctagct
     2701 ctcagaatga caattaataa acgagtattc atagcgatga ctgaatgaca catcgggaga
     2761 aaaaacaaac aatggatgaa aacaacaaca aaaaaacaca gaggaaaaag aaaagaagac
     2821 gaaataaaaa gcttatggtc agcacagcag cttttattac ggctgctgta gttgttgttt
     2881 tttgtatctg ctatgtatat tataaaactc catatttcaa ccttataggt gttgacattg
     2941 atggtaattt aacatatagt gatgaatata taatggaaaa atcaggtatt gaagtcggta
     3001 aaaaaatatt tgaagttgac aggtcaaagg ttaaagagaa tattgaaaat gaagtatacg
     3061 tggaaaatgc aagggtggtt tatgagcttc cgaataaaat attcctggat ataaaagaaa
     3121 gggtgaatac atatcaggtt ttcttcaaca acgaatacat aataatcgat aaaaatggaa
     3181 tagtattgag aacctgtaat gagaaattag acttattaac tattgaaagt tatacggatg
     3241 tagtatacaa tataggggat aatatacaat ttgcaggcgt tgaaaataat gaaaaagtat
     3301 tcaatgcaat agagtattta aacaatgaat atggaagcga tataataaaa gcctttaata
     3361 tcgaattgaa caacagcttc ctctttaaaa cacaatatgg cactgcagta aggatagact
     3421 taagcgagga cattaattat cagattgttt ttggaatgaa aataattaac gaaagattaa
     3481 acaataattt aacggtagct acggggttaa ttgattttac aaaaggtgac agtcctgtat
     3541 atattgagga ctaacggagg aaatatgaag aacaaaatct ggcaaaaagt aatgatgttt
     3601 tctataagct taatattagg gattatcttg gttactcaaa tgagaacaaa gagcaatata
     3661 ttgggcaccg ttaatttaga taaaaaagca gttgagctga atgaggaact ggctaagcta
     3721 agcatcgaaa aaaacagact tagggatgaa cttgaatcac ttaaaaaaga aagcagagaa
     3781 agtgaagaat tattcaagga tgctgaagaa gaactattaa agcttaaaga agaacttaaa
     3841 gttcagaaga taatatcagg ctattatgat gttaaaggac ccggaaccat aataaccata
     3901 gatgccgagc cggatacata tttaagtctt gcatccagtc atcagtatat attagcttta
     3961 atcagctatt taaacaatgc cggtgttgaa gctatttcca tcaatggaca aagatatacc
     4021 aactatacgg aaatagtgcc ggttcttgaa catattaacg taaacggtgt cgccatggta
     4081 cttcctttgg aaataaaggc aatcggagac agatctacaa tcaattcttc tcttaacttt
     4141 gtaggaggta tagtttctca acttaatgaa ataggatata taattgaaac ggaagattcg
     4201 gatgaaattt ttattccgcg ttatgacgga gaaaaacagt tcaagtatgc gcttccaata
     4261 attcgggatg aagaaatcta aggagtgagc actattaaaa aaacattaaa tataataatt
     4321 ttcgtgctgg tttttttgac agctgcagca attgtaaacg gtgcagatgc aggaatagat
     4381 gagtatacca tatatacaat cagagatttg aacaaatatg cacacagtat caaagaaatg
     4441 aagctcttgg taaataatat gcgcaatgct gtagaaagcg aaaaaagaac aattgccgac
     4501 tatgaatcaa aaaaatattt agacgacata attgaaatta aatctcaaat tgagcagatt
     4561 aaactatata atgggtttac ggctgtgcga ggaccgggta taatgctgag agtttcagac
     4621 aacatcaatg aagactacga ccttgagtta atggaaagaa tagtccatga tgtagatatt
     4681 gttgtactgt taaacgattt aaaggcagca ggagcggaag caattgaagt taacgggaaa
     4741 agaataatta acatatctga agtagtatgt gcgggacctt taatcagagt aaacggtgaa
     4801 gtaatacctg ctccttttgt cataatagca atcggagata tggatgaatt gtatgatgca
     4861 gttaccgaag aaggaactta tgcttacgag ttgaagaaaa cgtacggaat ggaagtagtt
     4921 gttgcacaag gatataattt aacggtcaga gaatttcgcg gaataaatta tgaaaccaaa
     4981 tatgccgaaa tagtagaaga aggggaaaag aaatgatata tgttgcaatc ggattaatct
     5041 taggtatagt tgcaggattg aacttaaatt ttatttacaa tccggattat atagtgtata
     5101 tatcactggc aatactggca atattaaata caattataaa tatacttcat gaaaataaaa
     5161 catcagaatt gacaattaca aaaagtattg cattcttgtt aacagacttg ttatttgctt
     5221 tgttcctggg atttgtgggc gagcaattag gtttgcctat ttatttagct gcagtatttg
     5281 cattcggtaa taatatttat aaaaagataa aagtcatagt cgatatatat cttgaaaagt
     5341 attttataaa caagtaaact caatattgtg ataattctga aaaaatattc ggaaaattgg
     5401 tgtagattat aaaaaatatg tatgaatatt attaaattta tgatagaata gtttaaatag
     5461 tatgaaaaat ttctctctca ggaggtcaaa atatgtttca atttgaggaa ccatcagaaa
     5521 gacttgccaa tattaaagta ataggtgtag gcgggggcgg caataatgca gtaaacagaa
     5581 tgatagatgc cggtgtcaga ggtgtgaatt ttatcagcat aaatacggac aagcaggctc
     5641 ttggcttatc aaaagcagaa acaaaatttc aaataggtga aaaattaact aatggattag
     5701 gagccggctc aaaaccggaa ataggagaaa aagcagctga agaaaataaa caagatatag
     5761 cagaactggt agaaggcgca gatatggttt tcataaccgc cggtatgggc ggaggaaccg
     5821 gaaccggtgc agctcctatt attgctaata ttgccaaatc tatgggtata cttacggttg
     5881 cagttgtaac taaacctttt atgtttgaag gaagatgcag aatgagaaat gctgaaatgg
     5941 gacttaaaag gttaaaggat catatagata ctttggttgt tattccgaac gacaaacttt
     6001 tggagataac agacaagaaa acaaccatgc tggaagcttt tgccatggca gatgaagtat
     6061 tgagacaagg tatacagggt atatctgatt tgatagcaat ccctgccata atgaaccttg
     6121 attttgccga cgttgaaaca attatgtccg gtcaaggtat tgctcacatg ggaatcggaa
     6181 tggcttcagg agaaaacaga gcattaaatg cagttaaaca ggcaatacaa agtcctcttt
     6241 tagaaacatc catcaatgga gcaagaggtg tgttggtaaa tattacgggt ggtccaagca
     6301 tgggaatcca tgaagttaac gaagcagcaa aattaatcga agaagctgct gacccggaag
     6361 caaacttaat atatggagca aatattgacg aatctgccgg agatactatt aaaattactg
     6421 ttattgcgac aggttttgca gacggagcaa aaaaatccga tggggaaccg agtgagaaaa
     6481 gcgaaaagaa accggaaaac ccgttcgatt ttacaatacc aagcttcttg aataaaaaat
     6541 aaaattttgg gtatcggtgc cgggcaccat ttgtggtgcc aggcaccgat aaaagaaccg
     6601 tcccaaaacg aatatccaat tgttccttga ttttccaaaa aaataattgt tgacatataa
     6661 agcttgatgt gttagtctta aaatgtatta cagatgtatt tatggatttc acatgttttt
     6721 agatattata tgtgg
//
LOCUS       DAOPUE010000091         6717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11336_length_6717_cov_10.340739, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000091 DAOPUE010000000
VERSION     DAOPUE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11336_length_6717_cov_10.340739"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKH42_07845"
     CDS             <1..1020
                     /locus_tag="PKH42_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOA20383.1"
                     /translation="QYDHIDTVEEWILQPSGQSQRRRTLLNLRRPFLNHNGVNNLNFS
                     PETGRLVLTTGDGGSGYDPFNLSQNIMEIAGKIIEIDVNTDILINNLPAVTRFNELPE
                     AVQRTLTVIAKGTRNIPGIAFQRYNNQYIKYVGNVGQDLVESVFSFTYYKPIPVTEII
                     SASTQNRSLNEDGLINLGWRGWEGDLPTPIINPCLSNPSLVEETIAYYNEAISVATKR
                     IFPLTCYYHMDPRPDKFSGTALTGYQPYMGSEIPDLTGCILFIDFVRRGSTPARGVLA
                     YTKVRTECKLNDYSIIEPNYNFGTQSAYYVSLGANLTQSRLYLGVYGSSNVTDFNQGT
                     VFEIV"
     gene            complement(1145..>2915)
                     /locus_tag="PKH42_07850"
     CDS             complement(1145..>2915)
                     /locus_tag="PKH42_07850"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOA20384.1"
                     /translation="YTLTEGTNEITTHVKIRTGTVIGNVTIEYTGSADLKLPAGLTVD
                     GDLTVIAPKATVNNYASVTGNIIIRDVASTTWNEYADGNILIVEDENGLVLHVVGNVR
                     GITVSESTDGNITIALSRDVNTDAIEFIAIKGPVTLITQKLIQATIKEGTTIIVKSSI
                     DGKEIEFTGTGEEVEILDGYPIVPVTINYLDEKTKEVIASVIEYSIDNGENWIAGPGS
                     ELDLIPGKDVIFRIAEMPSRELQTLIVPERPEGISFSYANSVSDYLMGLPAGATNIEA
                     RILAGERVYQDWTDITVDSDGKASLIHGGGDLIQVRTKAVTGSAFAGDIVQHYSKKSI
                     SELDIGDLVVDNSWQWEYKTGSGYSGTGELKPVTWIVAAKEHYGMDSGVTLLSKDLIG
                     QFTFDNRTDVHHNGVNLWGPSNHIRPWLNSVGIYEENGFYNAFSIEFMNSVLMTDVPN
                     EGYWEGKYTTQDKVFIPSITELGAPEVALEYYDEELDETFEIGITYVIGTVYPYFENA
                     SNSDRIAKLGASNFSYWTRSPEDWLKDIVHCILNDGSRGIYWGENLQAKDNNGVRPAV
                     NIAPDTLVSGIPNENGAYEIFYE"
     assembly_gap    2916..3015
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3016..3597)
                     /locus_tag="PKH42_07855"
     CDS             complement(<3016..3597)
                     /locus_tag="PKH42_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20385.1"
                     /translation="MKKGIILLLIITLILSLFQTAFAQIYDISNHWASKEINYLVERE
                     VLSGYPDGTFKPDNPITKAEFYKVINKLLNFEERADITYSDVNETDWYYEDVGKALAA
                     GYISPATVLLPNEYITRQEVARILGFVYNLDDESEAASVFSDSESILPEIRGYIGVLF
                     ENNLLHGYPDGTFKPSANITRAEVVKLIYNVLEK"
     gene            3780..4400
                     /locus_tag="PKH42_07860"
     CDS             3780..4400
                     /locus_tag="PKH42_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOA20386.1"
                     /translation="MEKKNINKRKLKAIETKNKIYEAARKLSLEHGIEKVSVDTIVEA
                     AGVSKGAFYVHFESKDALTAELVDEYTNKADIDYKAFLNSLPDNMPVLDILVLLAEEI
                     CDFIETNIGLENMRVLYKAHLTKTINTSSAMSYNRELYNLFTEVLEKGLMRGEIRQDI
                     PVNNMAKHLVLAIRGITYEWCIRYPDIDLKEYVLDHFKILLYGLES"
     gene            4559..5479
                     /locus_tag="PKH42_07865"
     CDS             4559..5479
                     /locus_tag="PKH42_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOA20387.1"
                     /translation="MKTKLMADSTCDLSQEILNRYNICLAPLVVSIEGKEYRDRIDIS
                     NDEFYKNLMTFKPYPTTSMPSPESYIACIKHAIEQGYEKILCICMSSGTSGSYQSALL
                     AKELFYEQNPKSNIQFEIIDSVSMSHGSGWLIMKCAMFIENGYTFEELIEYCEIIKYR
                     IKHFLSVDDLDSLIRSGRLSNTSGVIGKLLNVKPIMSMKNKKGTVVAKKRGIKQVYNY
                     YIQEFINRVDYEQTDFVIIGYTSDINKAENLKALLLKANYKGDIYIMQMGVAVGTHVG
                     LGGLSMFFVEKPHEDFINHMKEKIISHIHS"
     gene            complement(5543..6646)
                     /locus_tag="PKH42_07870"
     CDS             complement(5543..6646)
                     /locus_tag="PKH42_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HOA20388.1"
                     /translation="MNFLQLDTPALLIDKDIMLNNLKHMQDYANEKKVALRPHTKTHK
                     MSKLALLQEELGAKGITVAKVGEAEVMAANGLRDIFIANEIVGETKLSRVKKLAETID
                     ISFGLDSIAQADMVEKVFEGSKRANVLIEIEVGENRSGVIEEDDYIELLNYLKQCSNI
                     NLKGVFSHDGHSYSAENIEACKEIHLEAQRRTLRFANIANEMGFKLETVSIGSTPSLM
                     HNFPILEGVTEIRPGTYIFMDASQANAYGNVDRNAATILTTVISLPTPERVITDVGAK
                     GITMQARSKGFTATKGLGLIKGWPEVSIYAVYDEHAIIYNKKFHDSMKVGDKVEIIPN
                     HICPVVNLYETAYIIENGRVVDEIPVDCRGKLK"
BASE COUNT         2076 a         1289 c         1076 g         2176 t
ORIGIN      
        1 caatatgacc atattgatac ggttgaagag tggattttac aaccaagcgg tcaatctcaa
       61 agaaggcgta cactgctgaa tttaagaaga ccctttttga accataatgg tgtcaacaac
      121 ttaaactttt cacctgaaac gggaaggtta gtacttacaa ccggggatgg cggctcaggt
      181 tatgaccctt ttaatttaag ccaaaatata atggaaattg caggaaaaat tattgaaatt
      241 gatgtaaaca ctgatatact gataaacaat cttccggctg ttacacgttt taatgagctt
      301 cccgaagctg ttcaaagaac gcttaccgta atcgcaaaag gtacccgcaa tattccgggc
      361 atagcatttc aaaggtataa taatcaatat attaaatatg tcggaaatgt cggtcaggat
      421 ttggtagaat cagttttttc ttttacttat tataaaccta ttccggtaac agaaattatt
      481 tcagcttcta ctcaaaatcg tagtcttaat gaggatggtt taatcaatct cggttggaga
      541 ggatgggaag gtgatttgcc gacaccaatt ataaacccct gcttatcaaa tccttctttg
      601 gttgaggaaa ccatagctta ttacaatgaa gcaatcagtg tagcaaccaa acgcatattt
      661 cctttgacct gctattatca catggatccg cgtcccgata aattcagcgg aacagcactt
      721 acaggttatc aaccctatat gggcagtgaa attcccgact tgacgggctg cattcttttt
      781 atagattttg tcaggagagg aagcacacct gcaagaggag tattggctta cacaaaagtg
      841 cggacggaat gcaaactgaa tgattacagc attattgaac ccaattataa ttttggaact
      901 caatcagctt actatgtgag tttgggagca aatctcactc aatcaagact gtatttaggt
      961 gtctacggct catccaatgt aactgatttt aatcagggaa ctgtttttga aattgtataa
     1021 actaatcatg aattgattaa atgttaatca gtattcattt taataataaa agaccagtat
     1081 agataaataa tccaactggc ctttttttct atttataatc ttaatttatc ttgctgcagt
     1141 caacctattc atagaaaatc tcgtaagccc cgttttcatt cgggataccc gataccagtg
     1201 tatcaggtgc tatatttaca gcaggtcgaa caccattatt atcttttgct tgtagatttt
     1261 caccccaata aattccacga cttccgtcat ttaagatgca atgcactata tctttcagcc
     1321 aatcttccgg agaccgggtc cagtagctaa aattgcttgc tcctaattta gcaattctat
     1381 ctgaattgga tgcattttca aaatatggat aaactgtacc tattacgtaa gtaattccta
     1441 tttcaaaagt ttcatcaagt tcttcatcat aatattccaa agccacctct ggagcaccca
     1501 attctgtaat tgatggaata aacactttat cttgtgttgt gtatttgcct tcccaataac
     1561 cttcatttgg tacatcagtc attaataccg aattcataaa ttctattgaa aaagcattgt
     1621 aaaatccatt ttcctcatat atccccacac tattcagcca tgggcggata tggttgctgg
     1681 gtccccaaag attaactccg ttatgatgta catcagtcct attgtcaaag gtaaattgac
     1741 ctattaaatc tttagacaac aaggtcactc cggaatccat gccgtaatgt tcttttgctg
     1801 ctacaatcca agtaaccggt tttagttcac cggttcccga atatccgctg ccggttttat
     1861 actcccactg ccaagaatta tctacaacca aatctccaat atctaattca ctgatacttt
     1921 ttttggaata atgctgaaca atatctccgg caaaggcgga cccggttaca gcctttgtcc
     1981 tgacttgaat taaatctccc ccaccgtgaa ttaaacttgc ctttccatca gagtcaactg
     2041 ttatatcagt ccaatcctga taaacccttt cgccggctaa aattcttgct tctatattag
     2101 ttgcgcctgc aggcaacccc attaaataat cactgacact attagcatag gaaaatgata
     2161 taccttctgg ccgttccggt actataagtg tttgtaattc cctcgacggc atctcagcaa
     2221 ttctaaaaat aacatccttt ccgggtatca aatccaattc actgccgggt ccggctatcc
     2281 agttttctcc attatcaatg ctatactcaa taactgaagc tataacttcc ttagtctttt
     2341 catctaaata attaattgta accggaacta tgggataacc gtctaatatt tcaacttcct
     2401 cgccggtacc tgtaaattca atttcttttc catcaatact ggatttgaca atgattgttg
     2461 ttccttcctt aatagttgct tgtataagct tttgcgttat taatgtaaca ggtcccttga
     2521 ttgctataaa ttctatagca tccgtattga catctctgct taaagcaatt gttatatttc
     2581 catctgttga ttcgcttact gtaatacccc tgacatttcc aactacatgt aatactaagc
     2641 cattttcatc ttcaacaatt aatatattgc catctgcata ttcattccaa gttgtgcttg
     2701 ctacatcccg gattattata ttcccggtta cgcttgcata gttgtttaca gtagcctttg
     2761 gtgctattac ggttaaatcc ccatctacag tcaatccggc aggtaatttt aaatctgcac
     2821 tgccggtgta ttcaattgtc acgtttccaa tcactgtacc tgttcttatt ttaacgtgtg
     2881 tagttatttc atttgttcct tccgttaatg tatatnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnctttt caagaacatt gtaaatcagc ttaactactt cagctctggt
     3061 aatatttgct gaaggcttaa atgttccatc cggatatcca tgtaatagat tgttttcgaa
     3121 taatacacca atataacctc ttatttcagg caatattgac tcagaatccg aaaatacgct
     3181 tgcagcttct gactcatcat caagattata aacaaatcct aagattcttg ctacctcttg
     3241 ccttgttata tattcatttg gcaacaatac tgttgcaggg ctgatgtatc ctgctgccaa
     3301 tgcctttccc acatcttcat aataccagtc tgtttcattt acgtctgaat atgtaatatc
     3361 tgctctttct tcaaaattta ataatttatt aattactttg taaaactccg cctttgttat
     3421 tggattatcc ggtttaaaag ttccatccgg gtatccgctt aatacctctc tctctaccaa
     3481 ataattaatt tctttgcttg cccaatggtt acttatatca tatatttgag caaatgctgt
     3541 ttggaataaa gatagaataa gcgtaataat cagtagcaat ataatacctt ttttcataaa
     3601 tcttcctcct attatgactt gtctaataag tgaaacttaa gagtttcttt tttaatatcc
     3661 atctatacaa aaaatggaat atatgttata atttaaaaaa tgcagtataa tgactgcagt
     3721 cacataccgt agtcattata caatatttgt tccaatcagt caatcacaag gagggaagaa
     3781 tggagaaaaa aaatataaac aaaagaaaat tgaaagcaat tgaaacaaaa aataaaattt
     3841 atgaagccgc ccgaaaatta tctttggagc atggaatcga aaaagtaagt gttgatacca
     3901 ttgtagaggc ggcaggagta tccaaaggtg cattctatgt acactttgaa tcaaaagatg
     3961 cgcttacagc tgaacttgta gatgaatata caaacaaagc agacattgat tataaagctt
     4021 ttctaaattc tcttcctgat aatatgcctg tccttgatat ccttgttcta ttggcagaag
     4081 aaatttgcga ttttattgaa acaaatatcg gattagaaaa tatgagagtg ctttataaag
     4141 ctcacttgac aaagactata aacacatcat ctgcaatgag ttataacagg gagctgtata
     4201 atttgttcac tgaagtattg gaaaaagggc tcatgcgggg tgagataaga caagacattc
     4261 ctgttaataa tatggcgaaa cacttggtct tagctatcag aggcattaca tatgaatggt
     4321 gtatccgata tcccgatatt gatttgaagg agtatgttct ggaccacttt aaaatactgc
     4381 tttacgggtt agaaagttaa aaatgcaata tatctcttgt atatgcttac caatttaata
     4441 tacgagctgc taaaaattgt atttcaaaga attacgaaaa atgttgaata ttgtcgaata
     4501 ggatggtatg atagtgcaaa tatacaagca ctaaaatact ataagaacgg gagcttatat
     4561 gaagacaaaa ttaatggcag attcaacgtg tgatttatct caagaaattt taaatcgtta
     4621 caatatatgt ttagctccac tggttgtttc aatagaagga aaggaataca gagaccgaat
     4681 agatatttca aacgatgaat tttataagaa cctgatgact ttcaaaccat atccgaccac
     4741 ttcaatgcca agtcctgaat catatattgc ctgtatcaaa catgccatag agcaaggata
     4801 tgagaaaata ctctgtattt gcatgtcaag cggaacaagc gggtcttatc aaagcgcttt
     4861 actggcaaaa gaattatttt acgaacaaaa tccaaagtct aatattcaat tcgaaataat
     4921 agactcggtt agcatgagtc atggtagcgg atggcttatt atgaaatgcg ccatgtttat
     4981 tgaaaacgga tatacgtttg aagagcttat tgaatattgt gaaataataa aatacagaat
     5041 caaacacttt ttatccgtag atgatttgga tagtctgata cgcagcggaa ggttatcaaa
     5101 tacaagcggc gttataggca aattgctaaa tgtcaagccg attatgtcaa tgaaaaataa
     5161 aaaaggcact gtagtggcaa aaaaacgagg aattaaacaa gtttataatt attatattca
     5221 agaatttata aaccgggtag attatgaaca aactgatttt gttatcatcg gatatacatc
     5281 cgacattaat aaagcagaaa atttgaaggc cctgctcctt aaagcaaact ataagggtga
     5341 catatacatt atgcaaatgg gagtagctgt tggtactcac gtaggtttag gcggtctttc
     5401 catgttcttt gtcgaaaaac ctcatgaaga cttcataaat cacatgaaag aaaaaataat
     5461 aagtcatatt catagttagt gataaagggg atgttaagca catccccttt attttaaatt
     5521 caactattta cgttcactta tcctatttaa gctttcctct gcaatcaacg ggaatctcat
     5581 ccaccacacg gccattctca ataatatagg ctgtttcata caagtttacc acaggacata
     5641 tatgattggg aattatctct accttatctc cgactttcat tgaatcgtga aatttcttgt
     5701 tatatataat tgcatgttca tcgtatacag cataaatgct tacttccggc catcctttta
     5761 taagacccaa gccttttgtt gcagtgaatc ccttacttct tgcctgcatt gtaattccct
     5821 tggcaccgac atccgtaata accctctcag gtgtaggtag acttattaca gtagttaaga
     5881 ttgtagctgc attcctgtcc acatttccgt atgcattagc ttgagaagca tccataaaaa
     5941 tatatgtgcc cggtctgatt tcagtgactc cttccaatat aggaaaattg tgcattaaag
     6001 aaggagttga gccgatactg actgtttcta atttaaatcc catttcattt gcaatatttg
     6061 caaatcgcag ggttcttctt tgagcttcca aatgtatttc tttgcatgct tcaatatttt
     6121 cggctgaata agaatgacca tcgtgagaaa aaactccctt taaatttatg ttgctgcatt
     6181 gtttcaaata atttagaagc tctatgtaat catcttcttc aataactccg gagcggtttt
     6241 ctcccacttc aatttcaatt aatacatttg ctcttttgct gccctcgaat actttctcga
     6301 ccatatctgc ctgggctatg ctatcaagtc caaatgaaat atctattgtt tcagccagtt
     6361 ttttaacacg tgaaagcttc gtttcgccta caatctcatt agcaataaaa atatccctta
     6421 gtccgtttgc agccattact tccgcttcac caaccttagc caccgttatg ccttttgccc
     6481 ccaattcttc ctgcaataaa gctaatttag acattttatg tgtcttggtg tgaggcctta
     6541 atgctacttt tttttcattt gcgtaatcct gcatatgttt taaattattc aacataatat
     6601 ccttatcgat taacaaagcc ggtgtatcta attgtagaaa gttcatgttt tttctcctta
     6661 ctttaactga tattatcatt tatatcataa tacaatatgc cgttaacaat caacgga
//
LOCUS       DAOPUE010000092         6684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11405_length_6684_cov_11.766631, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000092 DAOPUE010000000
VERSION     DAOPUE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11405_length_6684_cov_11.766631"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKH42_07875"
     CDS             complement(<1..871)
                     /locus_tag="PKH42_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOA20389.1"
                     /translation="MKKNKLLLISAAALVVAASAYFLFFNDKSVEVSTMNTYKTNIIK
                     TIEVSGIINSKDIEVISLEPGKKVVKAYVKENDIVKKNQLLSELDADDLNISLQKAKL
                     SLEDLNTKLSDLNNNNNDLVILKNTVEKNKENYSKVSNDLALAQEDLQRAENLYAERV
                     ISKAEYEKYVSAVNNLKSSLKTQELNLYDSNVNYKDKTDQRTLDKLSLQSQIESIKLD
                     IESLNNKIEDTKIYSSIDGIVTEFPLKELQETLSGEKIIIHSNSSLELTALVSQEEAV
                     LIDEGQKSIVTIDG"
     gene            complement(1006..2262)
                     /gene="uvsE"
                     /locus_tag="PKH42_07880"
     CDS             complement(1006..2262)
                     /gene="uvsE"
                     /locus_tag="PKH42_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HOA20390.1"
                     /translation="MKIGYACLTVGVPGTKLRSCTMKNASPEVLEALIQSNLDSLNNV
                     LDYNINNGIKLFRISSDIIPFGSHPVNSLNWQEKFSSQLTEIGQKALFSGTRLSMHPG
                     QYTVLNSPDEKVVERAVDDLRYHAEFLDSLGLGEEHKIILHIGGIYGDKKAAMERFIH
                     QYNLLEANIRNRLVIENDDKQYNISDVLKIGFSQGIPVVFDNLHHQVNPDGKFTESEW
                     IKRCSATWKKSDGLQKIHYSQQDRGKRPGSHSATIDVSGFLQFYESIKGINADIMLEV
                     KDKNLSAIKCINSVSKPEIKNLEMEWSRYKYLVLEHSPNNYNKIRQILKNKKAYPILE
                     FYRLIDEAVIAPVKPGNAVNAAQHIWGYFKDSYNEDMRKKFEKTLLKTSKGESAIPMK
                     RVLWKMAEEQEQKYLLESLYFIEAAM"
     gene            complement(2401..2934)
                     /locus_tag="PKH42_07885"
     CDS             complement(2401..2934)
                     /locus_tag="PKH42_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20391.1"
                     /translation="MEGLVILIIWALINSFIRSANKKDNEGKTQKRTTQTKRKNIFST
                     FIEEIEKEMQSEKEKHRLEGPAQRTQPSQSARTTQWAKPPQPALPKKQEQPSMLSDLP
                     KSKVQTKPAPPPKPVGLEIDAIGGDEEIGGIKEARISKGSKNNLDLNLNRDILKGIIY
                     SEILSKPKSLRNMKIRC"
     gene            complement(2939..3928)
                     /gene="floA"
                     /locus_tag="PKH42_07890"
     CDS             complement(2939..3928)
                     /gene="floA"
                     /locus_tag="PKH42_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720829.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOA20392.1"
                     /translation="MPELLLTGGLAIFIIILLILFFTFVPVGLWITAFFSGVRVKIST
                     LIGMRLRRVVPSRIVNPMIKATKAGLDLNIDELEAHYLAGGNVNTLVDALIAAQRANI
                     PLVFERAAAIDLAGRNVLEAVQVSVNPKVIETPNISAVAKNGIEVIVKARVTVRANIE
                     RLVGGAGEETIIARVGEGIVTTVGSSAAHADVLENPDSISRTVLNKGLDSGTAFEILS
                     IDIADVDVGRNIGAKLQMDQADADKKIAQAKAEERRAMAVAKEQEMIAEVQAMKAKVV
                     EAESQVPLAIAAALKEGNMGVMDYYNMKNVLADTDMRDSISKATKEDNNKSGM"
     gene            complement(3930..5219)
                     /locus_tag="PKH42_07895"
     CDS             complement(3930..5219)
                     /locus_tag="PKH42_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOA20393.1"
                     /translation="MKLRKFGIIFIILLLLSSIALGAEVNTSDKVYVIPINGEINRAT
                     NYFVQNTIEELEREGAKAIIFEIDTYGGLIDQAQQIKDSIIGTDIPTISFVNNNAQSA
                     GVLITIASEKIVMAKNSTLGSAETIPNTEKVLSMWRAVLRDTARYRGRNSDIIEAMAD
                     KDIEIEGINEKGKLINLTSNEAVEYGIADLTANDYESILEYFNIQASEIVKVKEDFQV
                     KIAKAVTSSTLSTLLLAIGFIGIAVEIFTPGFGVGGIASIIAFGLYFGGNILAGNSNW
                     ISLTLFVIGLILIVVEGMVPGFGLPGISGIIFIVVGIIMAMNNLRSAVLSLSIAIILT
                     AIVVIILVKLGYRSKIFNNIILSNKLHKDKGYIPSEDMEHLLGEEGRALSELRPAGFI
                     EIGNNKYDALSDGGFITRNDKIKVVKVEGSKIFVRRI"
     gene            complement(5487..6602)
                     /locus_tag="PKH42_07900"
     CDS             complement(5487..6602)
                     /locus_tag="PKH42_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOA20394.1"
                     /translation="MKIVEGRFNSAVVFTDDLEDSSAAQIKTLCDQEYTKGSKIRIMP
                     DVHAGAGCTIGTTMTITDKLVPNLVGVDIGCGMETVKIKNSHIELSRLDKLIYEKIPS
                     GFSIRNAAHKYNEEIDLTRLRCIEGGKINLRRAILSLGTLGGGNHFIEAGRDEEGRTY
                     IVVHSGSRHLGFQVANFYQNQAYKYINKDIKTQIPKSLAYVSDKLMEDYIHDMKLVQK
                     FAMLNRKAIMDEIIKGMKLKAEDEFTTIHNYIDTENMILRKGAVSAQKGERLLIPINM
                     RDGSLICTGKGNSDWNCSAPHGAGRLMSRNQAKSSFTLSEFKKQMDGIYTSTVNNETL
                     DECPMAYKGIEDIIRNISDTAEVIKIIKPIYNFKAVE"
BASE COUNT         1816 a         1370 c          972 g         2526 t
ORIGIN      
        1 aaccatctat tgttacgatg ctcttctgac cttcatcaat taataccgct tcctcctgtg
       61 aaacaagagc ggtaagttcc aaagaagaat tgctgtgtat gattattttt tcaccgctta
      121 atgtctcctg taattctttc aagggaaatt cagttacaat accgtcaatt gaagaatata
      181 ttttcgtatc ctctatttta ttgtttaagc tctcaatatc aagtttgata gattctattt
      241 gactttggag ggaaagcttg tccagtgtcc tttgatctgt tttgtccttg tagttaacgt
      301 tagaatcata taaatttaat tcctgtgttt taaggcttga tttgagattg tttactgctg
      361 acacatattt ttcatactct gccttactga ttactctctc agcataaaga ttctctgctc
      421 tctgtaaatc ttcctgggca agagctaaat catttgatac ttttgagtaa ttttccttgt
      481 ttttttcaac tgtgtttttt aagattacca aatcattgtt gttattattt aagtcactca
      541 attttgtatt taagtcttcc aaactaagct ttgctttttg taaagaaata tttaaatcat
      601 cagcatccag ttcagataaa agctgattct tcttaacaat atcattttcc ttcacataag
      661 ctttaacaac ttttttaccc ggctccaagc taataacctc tatgtctttt gagtttataa
      721 tccctgatac ttcaattgtt ttgatgatat ttgtcttata ggtgttcatc gtgctgactt
      781 ctaccgactt gtcattaaaa aaaagaaaat atgcggaggc tgccacaact aaagctgctg
      841 ctgatatcaa taataatttg ttttttttca tactcgtctc ccttttatta tatatattca
      901 tttatgctca ctatacacaa ttctaacata aaataaaaaa tataaaagct tttaattgtt
      961 taaattataa gaattttcga taatcctacc ttattaaata agcttctaca tggctgcctc
     1021 tataaaatac agggattcca acagatactt ttgctcttgt tcttctgcca tcttccaaag
     1081 cacccgtttc atgggaatcg ccgattcgcc cttgcttgtt tttaagaggg ttttttcaaa
     1141 ttttttacgc atgtcttcat tatatgagtc cttgaagtaa ccccatatat gctgggctgc
     1201 atttactgcg tttccgggct taaccggggc tatcaccgcc tcatctataa gtctgtaaaa
     1261 ttcaaggatt ggataagcct tcttgttttt taaaatttgt cttattttat tgtaattatt
     1321 gggtgaatgc tccagtacca ggtatttata acggctccat tccatttcaa ggtttttgat
     1381 ttcaggcttg gaaacagaat ttatgcattt tattgccgat aggtttttgt ctttaacctc
     1441 aagcataatg tcagcattta tccccttaat actctcatag aactgcaaaa aaccactgac
     1501 atctatggta gctgaatggg aacccggccg ctttccccta tcctgctgtg aataatgtat
     1561 cttttgcagg ccatctgact ttttccaggt ggctgaacac ctctttatcc attctgattc
     1621 tgtaaattta ccgtcaggat taacctgatg gtggaggttg tcaaaaacta cgggtatccc
     1681 ctggctaaat ccgattttga gcacatcgga tatattatac tgcttgtcat cattttctat
     1741 aactaaacga ttcctgatat tagcttccaa aagattatat tgatgtataa aacgctccat
     1801 agcagctttt ttgtctccgt aaatgccgcc tatgtgaagg ataatcttat gttcttctcc
     1861 taagcccagt gaatccagaa attctgcatg ataccgcaaa tcatctactg ccctttccac
     1921 aaccttttca tcgggagaat ttaaaacagt gtattgacca gggtgcatag aaagacgcgt
     1981 gccgctgaat agggcttttt gtccgatttc tgtaagctgg ctgctgaatt tttcctgcca
     2041 gtttaagcta ttaaccggat gcgacccaaa aggtattata tctgagctta tgcgaaacag
     2101 ctttatgccg ttatttatat tatagtctaa cacattattt aaagaatcca ggtttgactg
     2161 aatcaaagct tctaaaactt caggagaagc atttttcatt gtacaggatc tgagcttcgt
     2221 tcccggaaca ccgacagtaa ggcatgcata accaattttc ataaatcacc taaatcttct
     2281 gaattttaca agatctccct tgttttttat taaaacttat atatgacagc tgccacattt
     2341 ttaataaaac agcccttagt tagattttag gtctaaataa gaggccggat tgttgttgtt
     2401 ttaacatctt atcttcatat ttcttaaact ctttggtttg gacaaaatct cagaatatat
     2461 gatgcctttt aatatatctc tatttaagtt taaatctaag ttattctttg atcctttaga
     2521 aattctggct tccttaattc ccccaatttc ctcgtctcca ccaatagcat caatttctaa
     2581 acctacaggt ttaggtggcg gtgctggttt tgtttggact tttgatttag ggagatctga
     2641 caacatggat ggctgttcct gttttttcgg aagtgccggt tgtggcggtt ttgcccattg
     2701 tgtcgttctt gccgattgcg acggttgtgt ccgttgtgcc ggtccttcta atctatgctt
     2761 ttctttttca ctttgcattt ctttttctat ttcttctata aatgtgctga aaatattttt
     2821 tctttttgtt tgagtagttc ttttttgggt ctttccttca ttgtcttttt tattagcgct
     2881 tctaataaaa gagtttatca aagcccagat aataagaatg accagtcctt ccatttaatc
     2941 acatcccact tttattgtta tcctctttag ttgctttaga aatagaatcc ctcatatccg
     3001 tatctgctaa tacatttttc atattataat aatccattac acccatattg ccttctttta
     3061 aagctgcagc gattgccaat ggcacctgtg attctgcttc tactactttt gctttcatgg
     3121 cttggacttc agcaatcatt tcttgttcct tagctactgc catcgctctt ctttcttcag
     3181 cttttgcttg tgcaatcttc ttgtcagcat ctgcttgatc catttgtagt ttcgcaccta
     3241 tgtttcttcc tacatctaca tctgcaatat caattgataa tatttcaaag gcagttccgg
     3301 aatctaagcc tttattaagt actgtccttg atatgctgtc aggattctct aatacatctg
     3361 catgagctgc agaagaacca acagtggtaa caataccttc gccgactctg gctataattg
     3421 tttcttctcc tgctccacct accagtcttt caatattagc ccttactgtt accctggctt
     3481 ttacaattac ttcaatacca ttcttagcaa ctgctgatat attaggagtt tctataactt
     3541 tagggtttac acttacttga actgcttcca atacatttct gcctgctaaa tcgatagctg
     3601 ccgctctttc aaataccaag ggtatattag ctctttgtgc tgctattaaa gcatctacaa
     3661 gtgtatttac attaccgcct gccaaataat gagcttctaa ttcatcaata tttaaatcaa
     3721 gacctgcttt tgttgcttta atcatagggt ttactattct gcttggtact acccttctaa
     3781 gcctcattcc aataagagta gatatcttaa ctctaactcc tgagaagaat gccgtaatcc
     3841 ataaaccaac cggtacaaat gtaaaaaata aaattagcag tataattatg aaaattgcta
     3901 atcctcctgt taataatagt tctggcatat taaatcctcc ttacaaatat ttttgatcct
     3961 tcaactttta ctacctttat cttatcattt cttgttataa aaccaccatc tgacaatgcg
     4021 tcatatttgt tattccctat ttcaataaaa ccggcaggtc taagttcaga taaagccctg
     4081 ccttcctcac ctaataggtg ttccatatct tcagatggaa tatatccctt atccttatgc
     4141 agtttattgc ttaagattat attattgaaa attttactcc gataacctaa ttttactaat
     4201 attattacca caattgccgt caatattatt gctatactta gtgataatac cgctgaacgc
     4261 aaattattca ttgccattat tatacctacg acaataaata ttatcccgga aattccgggc
     4321 aggccaaatc cgggtaccat gccttcaact actatcagga ttaagcctat tacaaataac
     4381 gtcagagata tccaatttga attgcctgct aagatattgc ctccaaaata taatccaaag
     4441 gcaattatac ttgctatgcc tcctactcca aaaccgggag taaatatctc cactgctata
     4501 ccaataaatc ctattgccaa taataaagtg cttaaggtag aacttgtaac agcttttgca
     4561 atcttaactt gaaaatcttc ttttacttta acgatttcac ttgcttgaat attaaaatat
     4621 tccagtatac tttcgtaatc attagctgtt aaatcagcta taccatattc tactgcttca
     4681 ttgcttgtca aattaatcaa tttgcctttt tcattaatac cttctatttc aatatcctta
     4741 tctgccatgg cttcaattat gtcagaattc ctgcctctat accgggcggt atctcttaat
     4801 acagctctcc acatagataa tactttttca gtatttggaa tagtttctgc agaacctaaa
     4861 gtagaatttt ttgccataac tattttttcg cttgctatag tgataagcac acctgctgat
     4921 tgagcattgt tattaacgaa tgaaatagtt ggtatatctg tacctattat agaatctttt
     4981 atttgttgag cttgatcaat tagtccgcca taggtatcta tttcaaaaat tatagctttt
     5041 gccccttccc gctctaattc ttctattgta ttttgaacaa aataattagt tgctctattt
     5101 atttcgccat taattggaat tacatatact ttatcacttg tattaacttc tgcaccaaga
     5161 gctattgaac ttagcaacaa taaaattata aaaataattc caaattttct taatttcata
     5221 tgagtatcac ctgcctttca tttaataatg ctggtctttt atcttatacc catatttctt
     5281 agtaaataac aatatttttt ctttgtaggt cataaattta atataacata atattttgtc
     5341 tcatagtgta aattttttat caaaaatagt ctgctgtttt taaccaccgg actagtgctc
     5401 tattctctct attcttttgg ctcttctctt ttgatattta aatgctcctt ttttataatt
     5461 agttaaattt tgatttattg cttttactat tcaactgctt taaaattata aattggtttt
     5521 attattttga tgacttcagc agtatcactg atgttcctga taatatcttc tattcccttg
     5581 taagccatgg ggcattcatc caaagtttca ttattcacag ttgatgtata aattccgtcc
     5641 atttgttttt taaactctga taatgtaaat gaagatttgg cttgattcct ggacattaat
     5701 ctgcctgccc cgtggggcgc cgaacagttc cagtctgaat ttcctttgcc ggtacaaatt
     5761 aaggacccgt cccgcatgtt tatcggtatc agaagccttt ctcctttttg tgcagatact
     5821 gccccttttc ttaaaatcat attttcagtg tcaatataat tatgtatggt agtaaattca
     5881 tcctctgctt tcagcttcat gccctttatt atttcatcca taatagcttt cctgtttagc
     5941 atggcgaact tttgaaccag tttcatatca tgtatatagt cttccatgag cttatctgat
     6001 acatatgcta atgattttgg aatctgtgtt ttaatatctt tatttatgta tttataggcc
     6061 tggttttgat aaaagtttgc aacctgaaat cccaggtgcc ttgaacctga atgcaccact
     6121 atataagttc tgccctcttc atccctgccg gcttcaataa aatgattgcc ccctccaagg
     6181 gtcccaagag aaagaattgc tcttcttaaa ttgattttgc ctccttctat gcatctaagc
     6241 cgcgtcaagt ctatttcttc attatatttg tgggctgcat ttctaatgct gaaacctgaa
     6301 gggatttttt cataaatgag tttgtcaagt ctgctaagct ctatatgcga attttttatc
     6361 ttaacagttt ccattccaca tccgatgtct actcctacca aattaggtac cagcttatct
     6421 gttatagtca tggttgttcc tatggtacat cctgcccctg catgaacatc gggcataatg
     6481 cggattttag aacctttcgt gtattcctgg tcacataagg ttttaatctg cgctgcagac
     6541 gaatcctcta aatcatcagt aaatacaacg gcactgttaa accttccttc tactattttc
     6601 atcttatcaa atctctctcc tatatccatg ggtggtttta tgtcagcttt tccataataa
     6661 aatccttttc tataaaaaat tcct
//
LOCUS       DAOPUE010000093         6603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11574_length_6603_cov_7.961515, whole genome shotgun sequence.
ACCESSION   DAOPUE010000093 DAOPUE010000000
VERSION     DAOPUE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11574_length_6603_cov_7.961515"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1255)
                     /locus_tag="PKH42_07905"
     CDS             complement(50..1255)
                     /locus_tag="PKH42_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20395.1"
                     /translation="MTSYKCNVVDISGKKRKLTHKAETKSEVIDYLKKNNYIIIDIEK
                     SAGSIDFNIFGKERIKSKDLAVFCKQLYAMLKAGVTLVNSLEILRQQTDNKHFAKIIG
                     QMYEDLQKGNTFSESLSRHKDAFPDIFISMAEAGELSGNIDLIMNRLSNHYEKEYKIE
                     NKVKSAMTYPIILALVSIAVVVFLLTTVMPTFVEMYTSSGVELPRLTQTLIKVSQTIK
                     QYWHIMLMAILGLTAGVISLNKVPKIKYKEDYYKLKLPLVKDLLLKISTSRFTRTLST
                     LMGSGVPLLQALETVSGVTGNAYIGSKVLEAREDVRKGLALSQPLRQQGIFPPMVHNM
                     IKIGEDSGAIEEILDKTADFYDEEVDTAITRMTTLLEPAMIVFMGIIIGFIVIAMLMP
                     MFDMVKTVQ"
     assembly_gap    1374..1473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1616..2665)
                     /locus_tag="PKH42_07910"
     CDS             complement(1616..2665)
                     /locus_tag="PKH42_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOA20396.1"
                     /translation="MNINELLERLIMMKGSDLHISVGCAPYFRVNGILLNTGSESMTP
                     EDTENLVKQLATEEQIKEINEVGDYDMSYAVSSLGRFRINIFKQRGSYSLAIRSVQME
                     IPTLNEINMPDVIYEFTKKQRGLIVVTGPTGSGKSTTLASMINIINNERKCHIITLED
                     PIEYLHKHNKSIVNQREYRSDFVTFASGLRSCLRQDPDVILLGEMRDLETMEIALMAA
                     ETGHLVFSTLHTTGSAKTIDRIIDVFPPHQQQQVSIQLANTLEGIISQQLVLNMEETE
                     RLAAVEVLVSTPAVRNLIREKKTHQIQNLIQTGAKFGMQTMDGSLLEMYNQKRISRQT
                     LLRSCIDMEYVMKSM"
     gene            complement(2676..4364)
                     /locus_tag="PKH42_07915"
     CDS             complement(2676..4364)
                     /locus_tag="PKH42_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOA20397.1"
                     /translation="MNNYRKSSRLGDILLSKKEITRVQLDKALAEQAESGKRLGETLI
                     SLGYISENTLLNTLSEQLGINIVNLDNYPISVDATRLISEKLAIRTNAIPLKITRDKL
                     TVAMSEPLNIFDIEDIEMESGKKAEVVLASKSQITNAIEKYMSSRSTEIAAQDFDKES
                     LKNEDFENLVEEIDLNVSNSPVVRLINSLIRQALKMGASDIHIEPFEKKLRVRVRIDG
                     ELQEILTPSKHTQSAIVTRVKIIAGMNIAEKRLPQDGRIEFKIDNTTVDLRISVMPTV
                     YGEKVVMRLLDRSNFLKSKGELGFTEHNMEMFNSILDSPNGVILVTGPTGSGKTTTLY
                     AALNELNTMNKNIITVEDPVEYKIEGINQVQVNTKAGLLFSTGLRSILRQDPDIIMVG
                     EIRDEETAEIAVRAAITGHLVISTLHTNDAPSTIMRLIDMGVKPFLVAASLRGIVAQR
                     LVKRICTNCKTAHEATEAEKKLLEVTGDTTIYKGRGCPKCFYTGYSGRIAIHEVLKVD
                     KEIREAIQSGEGIDRITEISRKTGMTTLKDNCRELVLSGITSIEEYSRVIYKLD"
     gene            complement(4619..5002)
                     /locus_tag="PKH42_07920"
     CDS             complement(4619..5002)
                     /locus_tag="PKH42_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOA20398.1"
                     /translation="MKSLIRLRMSSQDAHYGGNLVDGARMLGLFGDVATELLIRNDGD
                     EGLFVAYDNVEFLAPVYAGEFIEAEGEIVSTGNSSRKMVFEARKVIVPRTDISDSACD
                     VLEESIVVCRASGTCVVPKNKQRKK"
     gene            complement(5093..6304)
                     /locus_tag="PKH42_07925"
     CDS             complement(5093..6304)
                     /locus_tag="PKH42_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOA20399.1"
                     /translation="MFNKFYKNESGQTLILAALIMVMLIGIGALTLDVGAMTFQRSSL
                     QNAADAAALAGVQNMTSINDIKKAVIDYARSNGLKVREDGAPQGGDTITINTPYKGDS
                     NKVEVIISREVKHHLAGVFGLAKSNISVRAVAEKSGSLGAAFNYAVFHGSEDEPLKFN
                     SSSINVNGDVHSNHEIQFNSLYANIKGTLEAVSELKVNSATVDIEDICRVSSIAKNSS
                     TINVKGSTILNPAPVIEMPDFSDILKAEAQTGITYTGNKTFNSGNFSFNQPIYVDGNL
                     TLNSGNFYFDRPIFVEGKLTINSTHFIGKGIIYAKDDIKVNSASFATTAGTVSLYSGK
                     NIELNSMWADINGFMYAPNGTISMNSMSSKVNGSVIGNIIKLNSGFLKVDYGDGNYDG
                     LPDGGSVRLVE"
     gene            complement(6364..>6603)
                     /locus_tag="PKH42_07930"
     CDS             complement(6364..>6603)
                     /locus_tag="PKH42_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20400.1"
                     /translation="ANEALEYQLQEEYKKTIALINEKSGITIDRLIAQKNLTVAEAKI
                     AALEVQLLKTNKAVEVKEEMNILKREVTGQVQDEE"
BASE COUNT         1517 a         1438 c         1184 g         2364 t
ORIGIN      
        1 ttaccccttt tatggggcgt gaaacatatg tgttgatatt ttttgattcc tactgcacgg
       61 ttttaaccat gtcgaacatg ggcataagca ttgctattac tataaagccg attattattc
      121 ccatgaagac tatcattgca ggctccagga gggtagtcat ccgtgttatg gcggtatcta
      181 cttcttcgtc atagaagtct gccgtcttgt caagaatttc ctctattgct cccgaatctt
      241 cccctatttt aatcatgttg tgaaccatgg gagggaatat tccctgctgt ctgaggggtt
      301 gggacagggc aaggcctttt ctcacatctt cccttgcctc caaaacttta cttcctatgt
      361 atgcattccc cgttacgccg gaaacggtct ccagtgcctg cagcaagggc acaccgcttc
      421 ccatgagggt tgaaagtgtc cgggtgaatc ttgatgttga tattttgagc aataaatctt
      481 taacaagggg aagcttcagc ttataatagt cttccttgta tttgattttc ggcaccttgt
      541 tcagtgatat tacacctgct gtcaatccta atattgccat gagcataatg tgccaatact
      601 gttttatggt ctgacttact ttaatcaatg tctgtgtcaa gcgaggaagc tctactccgg
      661 agcttgtata catttcaaca aatgtaggca taaccgttgt aagcaggaat accaccaccg
      721 caatacttac aagggctaaa attatgggat atgtcatggc ggatttaacc ttgttttcga
      781 ttttatattc tttttcatag tggtttgaca agcggttcat tataaggtct atatttccgc
      841 ttaattcccc tgcttccgcc atgcttatga aaatatccgg aaatgcatcc ttgtggcgtg
      901 ataagctttc ggaaaatgta tttccttttt gcaggtcctc atacatctgt cctattattt
      961 tagcaaagtg tttgttgtcg gtttgctggc ggagtatttc aagactgttt acaagagtta
     1021 ctcctgcctt cagcatggcg taaagctgct tgcaaaacac cgccaaatct tttgatttga
     1081 ttctctcttt gccaaaaata ttaaagtcta tgctgcctgc tgatttttct atatcaatta
     1141 ttatgtaatt gttttttttc aggtaatcta taacttctga ttttgtttcg gccttatggg
     1201 ttaattttcg ttttttaccc gatatgtcta ctacattgca tttgtatgat gtcatatcta
     1261 ccttccttta tttattattc tgttttaagc gtgagattct tcgctacgct cagaatgaca
     1321 gtggttgaga ttcttcgctg cgctcagaat gacagtggtt gagattcttc gctnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagtggt tgagattctt cgctgcgctc
     1501 agaatgacag tggttgagat tcttcgctac gctcagaatg acagtggttg agattcttcg
     1561 ctgcgctcag aatgacagtg gttgagattc ttcgctgcgc tcagaatgac aagggttaca
     1621 ttgatttcat cacatattcc atgtcgatgc aggaccttaa cagcgtctgt ctcgatattc
     1681 ttttttggtt gtacatttca agcagacttc cgtccattgt ctgcatgccg aattttgccc
     1741 cggtttgtat caggttttgt atctggtgtg ttttcttctc ccgtataaga tttcttacag
     1801 ccggagtgga taccagtact tcaactgccg caagtctttc ggtttcttcc atattgagaa
     1861 ccaattgctg tgatataatt ccttccagtg tattagccag ttgtattgat acctgctgct
     1921 gctggtgcgg cggaaataca tctattatac ggtcgattgt ctttgccgag ccggttgtat
     1981 gcagtgttga aaatacgaga tgtccggttt ctgctgccat aagggctatt tccattgttt
     2041 ccaaatccct catttccccc aaaagtatta catcggggtc ctgtcttaag caggagcgaa
     2101 gtcctgaggc gaaggttacg aaatcacttc tgtattcacg ctggttcact atacttttgt
     2161 tatgtttatg caaatattct atggggtctt ctaatgttat tatgtggcac ttccgttcat
     2221 tgtttattat gtttatcatt gaagcaagtg ttgtggattt accgctgccc gtaggtcccg
     2281 taacaactat cagccctctt tgttttttgg taaactcata tatgacatcc ggcatattga
     2341 tttcattaag agtcgggatt tccatctgca cggagcgtat agccagggag tagctccctc
     2401 tttgcttaaa tatattgatt ctgaaacggc ctaagctgct taccgcatag gacatatcat
     2461 aatctcccac ttcgtttatt tcttttatct gctcctctgt ggctaattgt tttaccagat
     2521 tttccgtgtc ttccggagtc atactttcgc ttccggtatt tagcaggatt ccgtttaccc
     2581 tgaaataagg cgcgcagcct acggatatat gcaagtcgga gcccttcatc attattaatc
     2641 tttccaataa ttcattaata ttcatattgc cctcactagt ctaatttata tataacacgc
     2701 gaatattctt ctatggaggt tatgccggaa agtaccagtt ccctgcagtt gtctttaagt
     2761 gtcgtcatcc ctgttttccg tgaaatctcc gttatcctgt ctattccctc tccgctttga
     2821 attgcttccc ttatttcttt atcgaccttt aatacttcat gtattgcaat ccttccgctg
     2881 taacccgtat aaaaacattt cgggcagcct cttcctttgt aaatggttgt atctccggtt
     2941 acttccaaca atttcttttc cgcttctgtc gcttcgtgtg ctgtcttaca gtttgtacaa
     3001 attcttttta caagtctttg agctactata cctcttaagg atgcagctac caggaatggt
     3061 tttaccccca tatctataag tcgcataatt gtcgaaggtg catcgtttgt atggagggta
     3121 gatattacaa ggtgccccgt tatagctgct cttacggcta tttccgccgt ttcttcatct
     3181 ctgatttctc caaccattat tatgtccggg tcctgtctca gtattgacct gagtcctgtc
     3241 gagaataaca agcctgcctt tgtgtttacc tggacttggt ttattccttc tattttgtat
     3301 tcgaccgggt cttctaccgt tattatgttt ttgttcattg tgttaagctc gtttaatgct
     3361 gcataaagag ttgttgtttt tccgcttccc gtaggaccgg taaccaggat tactccgtta
     3421 ggcgaatcta atatggaatt gaacatttcc atattgtgct ccgtgaagcc taattctccc
     3481 tttgatttta ggaagttgct tctgtccaaa agcctcatta caactttttc gccgtagacg
     3541 gtgggcatta ccgatatacg caaatccacc gtggtgttgt ctattttaaa ttcgattctg
     3601 ccgtcctggg gaagtctttt ttctgcaata ttcattcccg ctattatttt aacccttgtt
     3661 actatggccg actgcgtatg ctttgaaggt gttagaattt cctgcagttc tccgtctata
     3721 cgaactctta ccctcagttt tttttcaaag ggctctatat gtatgtcgct ggctcccatc
     3781 ttcaaagcct gtctgataag ggagtttatc agccttacaa caggtgaatt gcttacattt
     3841 aagtcaatct cttcgactaa gttttcaaag tcttcatttt tcaaggattc tttatcgaaa
     3901 tcctgggcag ctatttcggt gctcctgctg ctcatatact tttctattgc atttgttatt
     3961 tggcttttgg atgctaatac tacttccgcc tttttgcccg attccatctc tatatcttct
     4021 atgtcaaata tattgagtgg ctcgctcatg gctacagtaa gcttatctct tgttattttc
     4081 agaggtatgg catttgtcct tattgccaat ttttcgctga ttaaacgggt tgcatccaca
     4141 cttatgggat aattgtccag atttactata ttaatgccca attgctcaga caaggtgtta
     4201 agtaatgtat tttcactgat atatcctaag cttatcaatg tttctccaag tcttttgccg
     4261 ctttccgcct gttctgcaag ggccttgtcc aattgtactc ttgtaatttc cttttttgaa
     4321 aggagaatat cacctaacct tgaagatttc ctgtaattat tcattatttc tccttgttta
     4381 tgtatttttc cccggactgt gttttcttaa gacctgtccg catcctatat aatacgcagg
     4441 attcgtcttt atttttatgt tgccttcatt atcaattgtc aatattaatt ataatttgtt
     4501 gtaattacaa ttctattctt tatgaattat atttaaatta tatttcaatt acattttaat
     4561 tacgccgtat cctaatacaa taaaaatccg caattgcgga ttttttatac taaatttctt
     4621 attttttcct ttgcttgttc tttggaacaa cacacgtgcc gcttgccctg cagacgacta
     4681 tggactcttc caaaacatcg catgccgaat cacttatgtc ggtacgcggt actattactt
     4741 ttcttgcttc aaacaccatt tttcttgaag aattgcctgt tgaaactatt tctccttctg
     4801 cttcgataaa ttcccccgca taaacgggtg ccaaaaattc gacattgtca tatgcaacga
     4861 acagtccttc atctccgtca tttctgataa gaagttctgt tgccacatcc ccgaataatc
     4921 ccagcatcct tgctccatca actaaattgc cgccgtaatg agcatcctgt gagctcatcc
     4981 taagccttat caatgatttc atttcttcct cctaaattat cctcttgcca cttgccctgt
     5041 gacaagctta tcttaacatt gaatttagaa gttgtcaatt ctaatcattt ttttattcta
     5101 ccagcctcac gctgccccca tcaggaagac cgtcataatt gccgtctcca taatcgactt
     5161 taagaaatcc gctattgagc tttattatat ttccaataac acttccattg acttttgaag
     5221 acatgctgtt catggaaata gttccgttag gagcgtacat aaatccgtta atatctgccc
     5281 acatactgtt gagttctata ttctttcccg aataaagact gactgttccg gctgttgtcg
     5341 caaagctggc gctgtttacc ttgatatcat cctttgcata aatgataccc ttgcctatga
     5401 agtgggtact gttaatggtt aactttcctt ccacaaatat gggacggtca aaatagaaat
     5461 taccgctgtt aagggttaag ttgccgtcta cgtagatagg ctgattaaaa gagaaatttc
     5521 cgctgttaaa tgttttgttg ccggtatatg ttatgcctgt ttgagcttcg gcttttaata
     5581 tgtcggaaaa gtccggcatc tcaattaccg gggcagggtt tagaattgtg cttcccttta
     5641 catttatggt gcttgagttc ttagcaatgc ttgaaacccg acaaatatcc tcaatgtcaa
     5701 cggttgcact gtttactttt aattcggata cggcttcaag cgttccctta atattagcat
     5761 aaagactgtt aaattgaatt tcatgattag agtgaacatc gccgttaaca ttaatgctgc
     5821 tgctgttaaa tttcaacggt tcatcttcgg agccgtggaa aacggcataa ttaaacgctg
     5881 cacccaggct gccggatttt tcggcaactg cccggacgga tatatttgat tttgccagtc
     5941 cgaaaacacc tgctaaatga tgcttcactt ctctgcttat aataacctcg accttatttg
     6001 aatcaccctt ataaggagtg tttatcgtaa ttgtgtctcc cccttgtggt gctccgtcct
     6061 ctcttacttt taatccattg cttctggcat aatctataac tgcttttttg atgtcgttta
     6121 tacttgtcat attctgtaca cctgccaatg cagccgcatc tgccgcattc tgcaggctgc
     6181 tgcgctgaaa tgtcatcgct cccacatcca aggtcaaggc accgattcca atcagcataa
     6241 ccattatcaa ggcagctaag attaatgttt gaccggattc gtttttataa aatttattaa
     6301 acatagtatt acgtcccccc taatgctatt taccatccgc cgacatcttt tgcgggcaaa
     6361 taactattct tcatcttgca cttggcctgt aacttctctt ttcaaaatgt tcatttcttc
     6421 ttttacttca accgctttat tcgttttaag aagctgtact tccagtgctg caatttttgc
     6481 ttctgcaacg gttaaatttt tctgtgcaat taatctgtct atggtaatac ctgatttttc
     6541 gttaattaat gcgattgttt ttttgtattc ttcctgcaat tggtattcca aagcttcatt
     6601 tgc
//
LOCUS       DAOPUE010000094         6586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11608_length_6586_cov_9.328434, whole genome shotgun sequence.
ACCESSION   DAOPUE010000094 DAOPUE010000000
VERSION     DAOPUE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11608_length_6586_cov_9.328434"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..530)
                     /locus_tag="PKH42_07935"
     CDS             complement(252..530)
                     /locus_tag="PKH42_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20401.1"
                     /translation="MTNNINGNTPSIKNSKSIDQTEIQNAKQNMNNVASKSVILGAEL
                     TANISNKSSLGNMTNSVPGSNSSTSVENQELQYVKSEIKKSEDKKGVF"
     gene            complement(702..1619)
                     /locus_tag="PKH42_07940"
     CDS             complement(702..1619)
                     /locus_tag="PKH42_07940"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOA20402.1"
                     /translation="MTVKRIITLLTLFLLNTVILVTAITRQEFNPLGGNINKSETNYQ
                     ILSVEEKNKIPILIYHNLTYDSSKWNDVTISPDKFREEMISLKKLGFTTIHFKDYIEY
                     REKRKKLPRNPIIITFDDGYYSNYKYAYPILKDLNMKATISVIGWSVGRQYNKDNITP
                     IARHFTWAQAKEMYDSGYIDIQNHSYDLHDLNSITKGTEKSQYETQAEYERRFREDTL
                     KQKKLIESNVGNEVIVYTYPYGIYNETNEKILKELGFKVTLTTESGISDLDNGLNILK
                     RINMSGIESKELINQILGFQNRNDITSNK"
     gene            complement(1884..2243)
                     /locus_tag="PKH42_07945"
     CDS             complement(1884..2243)
                     /locus_tag="PKH42_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOA20403.1"
                     /translation="MNEVDEYIKSFPAEVQERLTAIRNIIHELAPQATERICMRMPTY
                     DLNGKWLVHFAGYKKHIGFYPQPEGIAAFEEKLSGYKTSKGAVQFPLNKPLPIDLIRE
                     MVRFRVDEQTKQLSNNG"
     gene            complement(2275..2430)
                     /locus_tag="PKH42_07950"
     CDS             complement(2275..2430)
                     /locus_tag="PKH42_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20404.1"
                     /translation="MSMLENLNYIKENGIEKFIDNEESRWACPKCGNIVSVHRTECQK
                     CGEKIFG"
     gene            complement(2557..3213)
                     /locus_tag="PKH42_07955"
     CDS             complement(2557..3213)
                     /locus_tag="PKH42_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA20405.1"
                     /translation="MGPNAILMLDEITQSIELKKEMRVLDLGCGTGLTSVFLAKEYGV
                     QTFAVDLWITAADNYKRFLQMGVDDLVIPIHADAAVELPFADEYFDALISVDSYHYYG
                     NKDGYFEKHLKSLIKKGAPVAIAIPGTKQEMNGIIPDELKPFLDDEGFATLHCISWWT
                     QILEKHLDDFSIKEMDCTDTAWSDWLAADNPIAKLDIEMMKADNGKYLNLISITGKRK
                     "
     gene            3501..4145
                     /locus_tag="PKH42_07960"
     CDS             3501..4145
                     /locus_tag="PKH42_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018670008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA20406.1"
                     /translation="MKGKQNMYNYDRSQHNFMTPSWQNDCYSYLNPYSYFFSQNFRPE
                     YFEQMTNQYNPHYPIDNMPYVQNYDYSQNVNKGYVEEIKDYGKEPYVVNINEITKQNN
                     NYRSALWTGDHLQLTLMSIPVGGDIGLEVHPNADQFLRIEEGQGTVTMGDSKDNLTYR
                     ENVYDDYAIFIPMGKWHNVVNTGRIPLKMYSIYAPPLHPKGVIQKTKEEAHAAK"
     gene            4442..4630
                     /locus_tag="PKH42_07965"
     CDS             4442..4630
                     /locus_tag="PKH42_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20407.1"
                     /translation="MIDRRYETDMRNNYRRMEPEMRNEFNRRMEFDRRHEMDRREEFN
                     RRHDFDLRFPFWWLFFFL"
     gene            complement(4734..5333)
                     /locus_tag="PKH42_07970"
     CDS             complement(4734..5333)
                     /locus_tag="PKH42_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20408.1"
                     /translation="MYYYKGKIYLGSAQLGVYDIETGDEKILAQTYIYNVSVDESGIY
                     YWAVDKGEFRHIDFDGTSENVILKGGDYFNYSKGNLYYMGVSENKNGPCHVINCLDIT
                     SNKTTMLLEEANEYFNAHGEFNGITFKQYNENPETIDPQILVPNDQGEIFIGYNESVG
                     YVYTSGDYLFMHGSLRESIVQNGKLDCIARLDGSVTIWD"
     gene            complement(5535..>6586)
                     /locus_tag="PKH42_07975"
     CDS             complement(5535..>6586)
                     /locus_tag="PKH42_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20409.1"
                     /translation="FKDLVSVIAKIYTKITQTYENIKTKLIMAVASLCPIPQLRDEFA
                     KALTALVDYGLSSIGIPPKFPNFEQLAEGNIAYLAQVALTEAGVPANEITEEMTEKAA
                     EGIVNHFKEAGKESFDENPINAPFLKLDPKALYQPAYLEVELENNTDYPTVPGNLDLN
                     VEFRLNEQGLFATSYDPVGLNLTADTYEYGYNPTTAMYTAQEYWNHFVYGLNGYTVDY
                     KYGGIWDGVAVYEVFAPKVDVKLPVVPANSSVRIKVYLEPGGFSLTSRYPDAEPKLYE
                     DFYNMYLNNGGADYTWFKLSTSFPEARTFYSDEFKRKGVIMVLDPKTDYRYHMDNSMG
                     SSLGREIQMPVNIDW"
BASE COUNT         2049 a         1177 c         1027 g         2333 t
ORIGIN      
        1 taatttaaac atctatttag gatgcaatag ataattcgaa aaattattta agttcaagtt
       61 tttacatcac tctccaaagg aatatgccat taattctata tttctgattt accattgttt
      121 acaagaaaga ttcaagctat tactcttttt agcgaaattg ttgagatgaa ccgcttggtg
      181 tttcaccagg actaaaaaaa ggctattggc ataaaagcca agagcctatt ggaggaccta
      241 ttaataatta attagaatac tccttttttg tcttcactct tttttatttc acttttgaca
      301 tattgtaatt cttggttttc tacagatgta gaactatttg accctggaac agaattagtc
      361 atgtttccaa ggcttgattt attagaaata tttgcagtta gttcagcacc taaaatcaca
      421 cttttgcttg caacattatt catattctgc ttagcatttt gaatttctgt ctgatcaatt
      481 gattttgaat ttttaatact aggagtattt ccatttatat tatttgtcat attgttgcct
      541 ccatttatat tttaaaattg taagattagc ttacatgaat tagtatcttc acaaaagcat
      601 gagttatact atcagtatgt tttagaaaaa ttattaaagt taaccaatta tgatatttta
      661 aataatgaaa taaatcaata tctaaatcat ctatattatc cctacttatt tgaagttata
      721 tcatttctat tttgaaaacc taatatttga ttaattaatt ccttactctc tataccagac
      781 atgtttattc tttttaaaat gtttaaaccg ttatccaaat ctgatattcc actttctgtt
      841 gttaatgtca ccttgaatcc taattccttt aaaatttttt cattagtttc attataaatg
      901 ccatatggat aggtatatac tataacttca ttcccaacat tagattcaat cagttttttc
      961 tgtttcaaag tatcttctct aaatcttctc tcatattctg cctgagtttc atattgtgac
     1021 ttctccgtgc cttttgtaat gctgttcaag tcatgtaagt catatgaatg attttgaatg
     1081 tcaatatacc cactgtcata catttctttc gcctgcgccc aggtaaaatg tcttgctata
     1141 ggagttatat tatctttatt atattgtctt ccgacagacc agcctattac tgatatagtt
     1201 gctttcatat tcaaatcttt taatatcgga tatgcatatt tgtaattgct ataataccca
     1261 tcatcaaacg ttataataat cgggttgcgg ggaagctttt tccgtttttc tctatattct
     1321 atataatcct tgaaatgaat tgttgtaaac cctaactttt tcaaagagat catttcttcc
     1381 ctgaatttat ccggcgatat agtaacgtca ttccattttg aagaatcgta agtgagatta
     1441 tgatatatta atattggaat tttattcttc tcttcgactg ataaaatctg ataatttgtt
     1501 tcacttttat tgatatttcc accaagaggg ttaaattctt gccttgttat tgcagttaca
     1561 agaattactg tatttagaag aaatagtgtc aataatgtaa ttattctctt aactgtcatc
     1621 tatttaattc cttcctaaag ttaatgtcta atcttcaacg tctttgataa ataagataag
     1681 aactaaattt tattaaattt tatatattaa gagtatcaga tatttatgaa aatattatat
     1741 aatatatttt gaagttgaag ataaaattgg aaaactgatc aaatatttgc atcttgacgg
     1801 cgaaggtgga agaataagtg caaaaacaaa caaggcactc tatacaatgc agagtgcctt
     1861 gttaatttct attatactca ctttcagccg ttgtttgaaa gttgttttgt ttgctcatct
     1921 accctgaacc tgaccatttc gcgaatcaaa tctattggaa gaggtttgtt cagcgggaat
     1981 tgcaccgctc ctttcgaggt tttatagccg gatagttttt cttcgaatgc tgcaataccc
     2041 tcaggttgtg gataaaagcc aatgtgcttc ttatatccgg caaaatgaac cagccatttt
     2101 ccatttagat catatgtggg cattctcata caaattcgtt ctgttgcctg tggagctaat
     2161 tcatgaataa tatttctgat tgcggttaat ctttcttgta cttccgcagg aaagcttttt
     2221 atatattcat ctacttcatt catttgcaat acctcaatct tcattatttt tgcatcaacc
     2281 aaatattttc tcaccgcatt tttgacattc tgttctgtga acagaaacta tattcccgca
     2341 tttaggacat gcccatctgg attcttcatt gtcaatgaat ttttcaatgc cgttttcctt
     2401 aatatagtta aggttttcaa gcatgctcat gtgatatttt gttttgtatc ttttatcgag
     2461 ttgtttaagc ctctataagc aaaccccgaa tgaatagtct aaaatgaaca aggcactctg
     2521 cgaaatctca tgtaaaatgc ttgcttgttt ttattgttat tttctttttc ctgttataga
     2581 gataagattc agatacttgc cattatcggc tttcatcatt tctatatcca gctttgcaat
     2641 tggattatcg gcagcaagcc aatcactcca cgctgtgtcg gtgcagtcca tttctttaat
     2701 cgaaaagtcg tctaaatgct tttcaagaat ttgcgtccac catgaaatgc aatgcaatgt
     2761 ggcaaaacct tcgtcatcta aaaacggttt taactcatca ggtatgatgc cgttcatttc
     2821 ctgctttgtg ccgggtatag ctattgcaac cggtgcaccc ttcttaatca gcgattttaa
     2881 atgcttctca aaatagccat ctttgtttcc gtaatagtgg taagaatcaa cgcttatcag
     2941 tgcatcgaag tactcgtctg caaaaggcaa ttccaccgct gcatcggcat gaatgggtat
     3001 aaccaagtca tcaacaccca tttgcagaaa acgcttatag ttatcggcgg ctgtaatcca
     3061 taaatcaact gcaaatgttt gtacgccgta ttctttagcc agaaacaccg atgttaaccc
     3121 cgtgccgcag cccagatcta aaacacgcat ttccttcttg agttcaatac tttgggttat
     3181 ctcgtcaagc ataagaattg cgtttggacc cataaaattt tgcgaaagaa agcgcttatc
     3241 atacttttct gtacgatgat atttatacat aatttcccct cccatccatc tttgttcttt
     3301 tccttcttag tttgcgaaac gacagattgt attgtagatt ttttcattta ttatattaca
     3361 tatacaaaaa taattctagg aaaattcaaa aaaccaatga aaattaattc atagtccttt
     3421 ataaggtgac agacaatctt taaaaaaatt atcacattaa ataaaagtca tcatagatta
     3481 atggtataaa cttataaatt atgaaaggaa aacaaaatat gtataattat gatagaagtc
     3541 agcataattt tatgacgccg tcatggcaaa atgattgtta ctcgtacctg aatccatatt
     3601 cttatttctt tagtcaaaat tttagacccg aatatttcga acagatgacc aatcaatata
     3661 atccacatta cccaattgat aatatgccgt atgttcaaaa ttatgattat tcgcaaaatg
     3721 taaataaagg ttatgttgaa gaaattaaag attatgggaa agaaccatat gttgttaata
     3781 ttaatgaaat tactaaacaa aataataatt atcgttcagc cttatggacg ggtgatcact
     3841 tacagcttac tctaatgagc atacctgttg gtggagacat aggtttggaa gtgcacccta
     3901 atgctgatca attcttgcgt attgaggaag gtcaaggaac ggtaacaatg ggtgatagca
     3961 aggataattt aacatataga gaaaatgttt acgatgatta tgctattttt attcctatgg
     4021 gaaaatggca taatgttgta aataccggaa ggattcctct taaaatgtat tctatttatg
     4081 cacctcccct gcaccctaaa ggagtaattc aaaaaaccaa agaagaagcg catgcagcta
     4141 aataaaatat gaaagactat cttatgaaag catccaaaga ctactccagc tcagcacttt
     4201 gctgagcttt attaataata tatcgatgtg aagatttccc taacttctaa atgtacaaga
     4261 tttacaaatt aaaaaaagcc tggattatca ggaaatgttt actaatgtct cagcatttta
     4321 gtaacatggc atctttttta tcatatattg tagtaaatta aattttataa aagtaggtac
     4381 taaaatattt accatgagta ttttttgtca tttactttta caattcgaaa ggagtttgta
     4441 tatgattgat agaagatatg aaactgatat gagaaataat tatagaagaa tggaacctga
     4501 aatgagaaat gaatttaata gacgaatgga atttgacaga agacatgaaa tggatagaag
     4561 ggaagagttt aacagaaggc atgattttga tttgagattt cctttttggt ggttattttt
     4621 cttcctataa aatccttata ataacacccc ttaaaaaagg gtgttattat aaaagttgat
     4681 ttaattaaac aatttcacga aaataataaa tgattgaaca tcaattctat tgttcaatcc
     4741 caaatagtca cacttccatc aagccgggca atacaatcca gcttaccatt ttgcacaata
     4801 ctctcacgta gagaaccgtg cataaaaagg taatctccag aggtgtagac ataccccaca
     4861 gattcattgt agccaatgaa tatctcgccc tgatcgtttg gtacaagtat ttggggatct
     4921 attgtttcgg ggttttcatt atattgctta aatgtaatgc cattaaattc accatgtgcg
     4981 ttaaaatatt cattagcctc ttcaaggagc atcgttgttt tattcgaagt aatatccaaa
     5041 caatttatta cgtggcatgg gccgtttttg ttttcactga cacccatata ataaaggttg
     5101 cctttgctgt agttgaagta gtcgccaccc tttaaaatca cattttcgct tgttccgtca
     5161 aaatctatat ggcggaactc acctttgtca acagcccaat aatatattcc gctttcgtcc
     5221 acggatacgt tataaatata agtttgcgca aggatttttt catcgcctgt ttctatatca
     5281 taaacgccca actgtgccga tccgaggtaa atcttacctt tataatagta catgagtgat
     5341 taccttacaa tactcaatag ttgcggcaat tcatattaaa taagtataga cgtgttcctg
     5401 gataatttcc attaaactct gaaacctggt atgcttcaga acaaggtgag gattttgcaa
     5461 taacatcaga ccgccaagaa cccaagctta ttcctgattt ttaattaatg ggcgactaat
     5521 gtcgcccatt tttgttacca gtcaatattc acaggcatct gaatttctct tccaagactg
     5581 cttcccatgg aattatccat atggtatctg tagtcggtct tggggtcaag aaccattatt
     5641 actccttttc ttttaaattc atcactgtaa aatgttcttg cttcagggaa acttgttgat
     5701 aatttaaacc aggtatagtc tgcaccgcca ttattcaggt acatattgta gaaatcttcg
     5761 tatagtttcg gttctgcatc aggatacctg ctggttaaag aaaatccgcc aggctctaaa
     5821 tatactttta ttctgacaga gctgtttgca ggcactacag gaagcttaac atctactttt
     5881 ggagcgaaaa cttcgtaaac tgccactcca tcccaaattc ctccatattt gtaatcaacg
     5941 gtatatccgt taagtccata aacaaaatgg ttccaatatt cttgtgcagt gtacattgct
     6001 gttgttggat tgtaaccata ttcataagta tctgcagtaa ggttaagtcc tacaggatca
     6061 tatgaagtag caaacaaacc ttgttcattt aacctaaatt cgacatttaa gtccaagtta
     6121 cccggcacag ttgggtaatc agtattgttt tcaagttcca cttcaaggta tgccggctga
     6181 tacaatgctt ttggatctaa ctttaagaat ggagcattta ttggattttc atcaaaactt
     6241 tctttccccg cttccttaaa atgatttaca attccttctg ctgccttctc ggtcatttct
     6301 tcggttattt cgtttgccgg aacacctgcc tctgtaagtg ctacctgagc aagatatgct
     6361 atattccctt ctgccaattg ttcaaagttt ggaaacttag gaggaattcc aattgatgac
     6421 aatccataat caacaagagc tgtaagtgcc ttagcaaatt catcccttaa ttgcggaatc
     6481 ggacagaggc ttgcaactgc cataattaat tttgttttaa tattttcata ggtttgagta
     6541 atcttggtat aaattttagc tatgactgat actaaatcct taaaga
//
LOCUS       DAOPUE010000095         6465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11897_length_6465_cov_8.769111, whole genome shotgun sequence.
ACCESSION   DAOPUE010000095 DAOPUE010000000
VERSION     DAOPUE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11897_length_6465_cov_8.769111"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1190
                     /locus_tag="PKH42_07980"
     CDS             15..1190
                     /locus_tag="PKH42_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOA20410.1"
                     /translation="MNEVVIGLIGSGYAGFLHVNGYKKVYGIPVRIKTIVDVDIEKAK
                     IFAKTHCIENASMNIEDVLNDPEIQIIDIATPPMTHIDIAAKALKAGKHVICEKPLSG
                     YFGQEGDVAPIGKTVPKEKMFKKVMEEIDEFKKVVEESGKLFMYAENYIYCPSVTKAA
                     EIIAHKKSKLLFMKGEESLRGSSSPVAGRWDKTGGGSFIRVGTHPLSGILWLKQVEAK
                     ARGEKINVVSVSADMGNTASMLNQYDRRHLTLETYDVEDFANVTLTFSDNTKAIVIGA
                     DNVLGGTKNYIEIYANDTSLMCNITPANNLQSYFLDEDNLEDVSISEMLPGKTGWNNV
                     FICDEILRGYTFEMQDFVESVAYNRQPKSGIELAYETTKILYAAYQAAYEQKTVFLV"
     gene            complement(1271..2218)
                     /locus_tag="PKH42_07985"
     CDS             complement(1271..2218)
                     /locus_tag="PKH42_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOA20411.1"
                     /translation="MKYLSKPFNNGKINLKNRLIMPPMATAKSETNGRITQDLIDYYD
                     EKSKGGYISLIIIEHSYISEDGKASKNQISIAEDSVIDDLRKLSDVIHKNGSKTVMQI
                     NHAGSRALEVGNSIHRGPLIDKDEIKEIVYNFRKAAIRVKEAGFDGVEIHSAHNYLLN
                     QFLSPITNKREDEYGGNIHGRIRIHLDVIKAVREAVGEEFPILIRLGATDDDVEGLTL
                     EDAVEAAKCLEKAGVDMIDVSGGMCRYMPSNSNEQGYFSTQSEEIKKAVNIPVMLTGG
                     ITDPYIADRMLENGKADFIGVGRSILYDSLWAKRAMELQ"
     gene            2454..3518
                     /locus_tag="PKH42_07990"
     CDS             2454..3518
                     /locus_tag="PKH42_07990"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOA20412.1"
                     /translation="MDLFSKMEEYDYEQLILCQDKSTGLKAIIAIHDTTLGPALGGTR
                     ICEYESDEEAIEDALRLARGMTYKNAAAGLNLGGGKAVIIGDPRKIKSEALFRAFGRF
                     VEGLNGRYITGEDMNATQEDASYINCETEYIVGLETGSGNPSPVTAYGVFKGIQAALN
                     EVYGSDDLTGKTVAIQGLGAVGRRLAERLHEAGAKLYVTTRDKAKIDKAVAELGATAV
                     SLDEIYGVECDVFSPCARGAIINDKTIEQFKCKIIAGAANNQLAESRHGDMLHEKGIL
                     YVPDYIINSGGVINIIDDISGREYNRENAMKNTARIYDSCKKVFEIAKRDNIPTYKAA
                     DIMAEERIAAVGRIKKIYNR"
     gene            complement(3571..4212)
                     /locus_tag="PKH42_07995"
     CDS             complement(3571..4212)
                     /locus_tag="PKH42_07995"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOA20413.1"
                     /translation="MESISCQEMKEMDSYAINTVGIPSIVLMENAALKVINNIDLNYN
                     NYTVVCGRGNNGGDGLAIARHLIIKGKKVRIYIAGDLKSGTEDFNINLNILKNLKAEI
                     INIKDEKELLQLKEDLSVSDITIDALFGIGLKRNIEGLYLEIIKMINNESKQIISVDI
                     PSGIDGDTGKPMGICVKADKTVTFHKMKKGLLNCGNYTGEIIVEDIGIPNIQF"
     gene            4523..5074
                     /locus_tag="PKH42_08000"
     CDS             4523..5074
                     /locus_tag="PKH42_08000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20414.1"
                     /translation="MNDTKLVNLFKNNPSKGLEAAVQKYSGLVKTIVVRIIGYDNQRD
                     VEETVSDVFVELWKSIDNFDPKKGQLKNYIISIARFVSINTYNRKISKHILIPLEEDN
                     LEFDMDLDNEVSKSINKEIINDTINNLPHPDKEIFIRRYYLFEPVKEIAEYLNLTPKT
                     VENKLYRGKDKLRTALINKGIIL"
     gene            5081..>6465
                     /locus_tag="PKH42_08005"
     CDS             5081..>6465
                     /locus_tag="PKH42_08005"
                     /inference="COORDINATES: protein motif:HMM:NF025159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HOA20415.1"
                     /translation="MTKLTKIHEIIDDIDIENEDLKIAESKVSKEEKNRILEMALNKA
                     NIRKEKTFSRKYILSLAAAMTLILSFAVVFAQGGLSNIYYKLFGENIKYVTEMGTIID
                     ESHSSNGITFNVSNMLGDENSFYIIFEIIKENGESFKDSDYIEFDRFSLDFKSSGGYT
                     WYQIEDDDKNDNIATFILSGNTKNKISGNKLTISATDITEYSIKEPAVKFDPYNFLID
                     NQDFIEQNLEKNLQKSTPDIKNHDNYSKEEIDKIEEIYRLTPNYVLPWKYLNIPADEG
                     LNEIYIDNIGFAENKLCIRIALSDKEMHSLGEIYFANKNSPEDILYNEFMLSEEKNGI
                     KYYYYIFNIENMEELKNYNFIYDIVSKLSTTRGEWNVTFKADYKNTTKTIKVNKETKI
                     NDKRYTVKNIKISPIALNVELKNNFSDNYEDPVHNFSEAISVIMKDGTKIEPTSSGSG
                     TNPLTSYIYLM"
BASE COUNT         2256 a         1031 c         1155 g         2023 t
ORIGIN      
        1 aaggtggtat ttttatgaat gaagtagtaa ttggattaat cggcagcgga tatgcagggt
       61 ttttacatgt aaacggatat aagaaggtct atggaattcc cgttaggata aagactatag
      121 tagacgtgga tattgaaaag gcaaaaattt ttgctaaaac ccattgcata gagaatgctt
      181 ctatgaatat tgaagatgtc cttaatgatc cggagattca aattattgac attgcaactc
      241 ctccaatgac ccatattgat atagctgcaa aagccttaaa agccggcaaa catgttattt
      301 gcgaaaaacc attatccggt tattttggac aagaaggaga tgtagctcca ataggtaaga
      361 ccgttcctaa agaaaagatg tttaagaagg taatggaaga aattgatgaa tttaagaaag
      421 tagttgaaga aagcggcaaa ctttttatgt atgctgaaaa ttacatatat tgtccttctg
      481 ttacaaaagc tgcagaaata attgcccata aaaaaagcaa attattgttt atgaaaggcg
      541 aagaaagttt aaggggttca tcctcacctg ttgccggaag atgggataaa acaggtggcg
      601 gctcatttat tagagtcgga actcatcctc tttcgggaat attgtggctt aaacaagtag
      661 aagccaaagc gagaggtgag aaaatcaacg ttgtcagtgt atcagctgac atgggtaata
      721 ctgcttctat gcttaatcaa tatgatagaa gacatttgac ccttgagact tatgatgtag
      781 aggattttgc aaatgtaact ttgacttttt cagacaatac taaggcaatt gtaattggag
      841 ctgacaatgt tctaggaggc accaagaatt atattgaaat ctatgcaaat gatacatcgc
      901 ttatgtgtaa tataactccg gcaaataatc tgcagtccta tttcttagac gaggataatt
      961 tggaagatgt aagtatttca gagatgcttc cgggaaaaac aggctggaat aatgtattca
     1021 tctgcgatga gatacttcgt ggatatactt ttgaaatgca ggattttgta gaaagtgtag
     1081 cttataacag gcaaccgaaa tccggaattg aattggctta tgaaacaaca aagatacttt
     1141 atgcagctta tcaggcagcc tatgagcaaa aaactgtatt tttagtttag ataccacctt
     1201 gtatagagta cacaagaaca aagtggtgcc aggcaccggt tgcgtttggt gcctggcgtc
     1261 ttttacgccc ctattggagc tccattgctc ttttcgccca taaagaatca tacaatatag
     1321 atcttcctac accgataaaa tctgcttttc cattctcgag cattctatcg gcaatatatg
     1381 ggtctgttat tccacctgtt aacataacag gaatattaac ggccttttta atttcttcag
     1441 actgagttga aaaatatcct tgctcattgc tgttactagg catatatctg cacattccgc
     1501 ctgaaacatc aatcatatcc actccggctt tttcgagaca ttttgctgct tcgacggcat
     1561 cctccagcgt taatccttca acatcatcgt ctgtggcacc aaggcgaatc aaaatgggaa
     1621 attcttctcc tacagcttct cttactgcct taataacatc taaatgaatt cttattctgc
     1681 catgtatatt tccgccatac tcatcttctc tcttatttgt tataggagat aaaaactgat
     1741 ttaagagata attgtgagca gaatgtattt cgacaccgtc aaagcctgct tccttgacac
     1801 gaattgcagc cttcctaaaa ttataaacaa tttctttaat ttcatccttg tctattaatg
     1861 gtcctctatg aatactgtta cccacttcta atgcccgact tcctgcatga ttaatctgca
     1921 tcactgtttt tgaaccattc ttatgtataa catcggataa ttttcttaaa tcatctatca
     1981 cactgtcttc agcaatagaa atctggtttt tggatgcctt cccatcctcc gaaatatagc
     2041 tgtgttcaat tattattagt gatatgtacc ccccttttga tttttcatca taataatcaa
     2101 ttaagtcttg ggttatcctg ccattggttt ctgactttgc agtagccatt ggcggcatta
     2161 tcagcctgtt ttttaaatta atttttccgt tattgaatgg ttttgataaa tatttcatag
     2221 atatcttctc cttttttatc actgtgcctt tcagtttggt gcctggcacc atcggcacca
     2281 caaaggctaa agcctttgct tcattgattg tattcattat atcatggcac gaagttattt
     2341 caataaaatt aacatatttt gaggtgccgg gcacttttaa tgtttgctgc atttttgttg
     2401 acatatatgt tataataaat aaattaatat cgtcataaga ggaggaacgc tatatggatt
     2461 tatttagtaa gatggaagaa tatgattacg aacaattaat actttgtcaa gataaaagta
     2521 ccggattaaa ggcaattatt gctattcatg atactacatt aggtccggca ctaggaggaa
     2581 caagaatttg cgaatatgag tctgatgaag aagcgattga agatgcgcta agattagcca
     2641 ggggtatgac atataaaaat gcagctgcag gattaaacct tggcggcggg aaagcagtta
     2701 ttataggaga cccaaggaag ataaaaagcg aagcattgtt tagagctttc ggcagatttg
     2761 tcgaaggttt aaatggcaga tacataaccg gagaagatat gaatgcaaca caagaagatg
     2821 cttcatatat taattgtgaa acagaatata tagtaggttt agaaaccgga agcggcaatc
     2881 cttcacctgt aaccgcttat ggagtattta aaggaataca ggcagcttta aatgaagttt
     2941 acggcagcga tgatttaacc ggaaaaaccg tagcaattca aggtctaggt gccgtcggca
     3001 gacgtttagc cgagcgttta catgaagcag gtgctaaatt atacgttact acaagagata
     3061 aggcaaaaat tgataaagct gtagcagaac tgggtgcaac agcagtaagc ctggatgaaa
     3121 tatatggtgt tgaatgtgat gtattttcac cttgtgctag aggtgctata ataaacgata
     3181 aaacaataga acaatttaaa tgtaaaatca tagccggcgc agccaacaat cagttagctg
     3241 agtcaagaca cggtgatatg ctgcatgaga aaggaatact ttatgtgccg gattatatta
     3301 taaacagcgg cggtgtgatt aatataattg atgatatatc gggacgtgaa tacaataggg
     3361 aaaatgcaat gaaaaatacc gcaagaattt atgattcttg caagaaggtg tttgagatag
     3421 ctaaaagaga taatattcca acatataagg ctgctgatat tatggcagaa gagagaatag
     3481 cagcagtagg aagaattaaa aaaatctata atagataaat ctaaaattta gtattaaacc
     3541 aatcaaccgt aaatttcgga agattggttt ttaaaattgt atatttggta tgcctatatc
     3601 ttctacaata atttcgccgg tatagttacc gcaatttaac agtccctttt tcattttatg
     3661 aaatgtaacg gttttgtcag cctttacgca aatacccatt ggctttcctg tatccccgtc
     3721 tattccggat ggaatatcaa ctgatataat ttgttttgat tcattattta tcattttaat
     3781 tatttcaagg tacaaacctt caatatttct ttttagacca attccaaaca atgcatcaat
     3841 tgtaatgtcg ctgacagata aatcttcctt taattgcaga agttcttttt catcttttat
     3901 atttatgatt tccgccttaa gattttttaa aatatttaaa ttaatattaa aatcttctgt
     3961 gccgcttttt aaatctccgg caatatatat ccttactttt ttacctttta taattaaatg
     4021 gcgagcaata gcaagtccat caccgccgtt gtttcctctt ccgcatacaa cagtataatt
     4081 attataattc aaatcaatat tatttataac tttaagcgct gcattttcca ttaatacaat
     4141 acttggtata ccaactgtgt taatggcata gctgtccatt tccttcattt cttgacaact
     4201 tatcgattcc ataatccatt ctcccttatt actctcaatc tcagaaattt caggtgcctg
     4261 ctcatcaaat ttttcctcgg tttcctaccc atctgtgcag cacctccgat aaaaatactt
     4321 attttttatt atataaaatt ttagccgaat aaacaagcgt gatttgttat aattttccat
     4381 tttggtgcct ggcaccgaca tgtgcaaaac ggtgcctggc accaattagt atgaccaatt
     4441 agtaaaaaac ttcttcattt tgagtgaaat tcaattataa tgtgaatata tatatatgaa
     4501 aagcttctta taaggagagc atatgaacga tacgaaatta gttaatcttt ttaaaaataa
     4561 tccttccaaa ggtttagaag ctgctgtgca gaaatattcg ggattggtaa aaacaattgt
     4621 tgtaagaata ataggatatg ataatcagcg ggatgttgaa gaaactgttt cagatgtatt
     4681 tgttgaactt tggaagtcca tagataactt tgaccctaaa aagggccagc taaaaaacta
     4741 tataatttca atagcgcgtt ttgtaagtat aaatacttat aaccgtaaga tttcaaaaca
     4801 tattttaatt cctctggaag aggataattt ggaatttgat atggatttag ataatgaagt
     4861 aagtaaatcc attaataaag aaattattaa tgatactatc aacaatcttc cccaccccga
     4921 taaggaaata tttatcagga ggtactactt gtttgaaccg gttaaggaaa tagcagagta
     4981 tcttaactta actccgaaaa ctgttgaaaa taaactgtac aggggtaagg ataagctaag
     5041 aacggcgctt attaataaag gaataatttt ataagaaagg gtgactaaat tgacaaaaat
     5101 tcatgagata atagatgata tcgatataga aaatgaggat ttaaaaatag cggaaagcaa
     5161 agtatcaaaa gaagaaaaaa acagaatttt agaaatggca ttaaataaag caaatataag
     5221 aaaagaaaaa actttcagcc gaaaatatat attatcatta gctgcagcaa tgactttgat
     5281 tttatcattt gccgttgttt ttgcccaagg cggattatcc aatatttatt ataaactgtt
     5341 tggtgaaaat attaagtatg tgaccgagat gggaactata atcgatgaaa gccattcatc
     5401 aaacggtatt acatttaatg tttcaaatat gttgggagat gaaaattcat tttatataat
     5461 atttgaaata atcaaagaaa atggagaaag ctttaaggat tccgattata tagagtttga
     5521 tagattcagt ttggatttta aaagctcagg cggatatacc tggtatcaaa tagaagatga
     5581 tgataaaaac gataatatag ctacattcat tttgtcgggc aatacaaaaa ataaaatatc
     5641 cggcaataaa ttgacaataa gtgcaaccga tataacggaa tacagcataa aggaaccggc
     5701 agttaaattt gacccctata actttttaat cgataaccaa gattttatcg agcaaaacct
     5761 tgaaaaaaat ctacaaaaat ctacaccaga tataaaaaac catgataatt attcaaaaga
     5821 agaaattgat aaaattgaag aaatttatcg attaacaccg aattatgtat tgccttggaa
     5881 atatttaaat attcctgccg atgaaggttt gaatgaaatt tacattgata atataggatt
     5941 tgcggaaaac aagctctgta tcaggattgc attatctgat aaagaaatgc atagtctggg
     6001 tgaaatatat tttgcaaata aaaatagccc tgaagatata ttgtataatg aatttatgct
     6061 ttcagaagaa aaaaacggca tcaaatatta ctactatatt tttaacatag aaaatatgga
     6121 agaattaaaa aactataatt ttatttatga tattgtaagt aaattaagca ctacaagggg
     6181 agaatggaat gtaactttta aagctgatta caaaaacacg acaaaaacca taaaggtaaa
     6241 taaagagaca aaaataaacg acaaaagata tacagttaag aatataaaaa tttctcctat
     6301 tgcactcaat gtagaattaa aaaataattt ctcggataac tatgaggacc ctgtgcataa
     6361 ttttagcgaa gcaatatcag taataatgaa agatggaaca aaaatagagc cgacaagcag
     6421 cggttccggc accaatccct taacatcata tatataccta atgtt
//
LOCUS       DAOPUE010000096         6455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_11923_length_6455_cov_7.697344, whole genome shotgun sequence.
ACCESSION   DAOPUE010000096 DAOPUE010000000
VERSION     DAOPUE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11923_length_6455_cov_7.697344"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKH42_08010"
     CDS             complement(<1..670)
                     /locus_tag="PKH42_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoFVII family restriction endonuclease"
                     /protein_id="HOA20416.1"
                     /translation="MSLNNGFYESVINKLINEEIYKIKQTKEYHAETKKIDEEEGSSI
                     LSKYMSIVINKSLNRIPNKDKLNKQIEICNKIIMLLVAELELNDFNEFIISENAELLL
                     SVLNCIDNSLIEKNKNNKIRPITSIAMNSLFTGAKNEPSLGSELVKEINTADRIDMLV
                     SFIKWSGLVQIYEALKEFTKEHKLRIITTSYMGATDYKAILELSKLPNTEIKISYDTK
                     RTRLH"
     gene            complement(891..1967)
                     /locus_tag="PKH42_08015"
     CDS             complement(891..1967)
                     /locus_tag="PKH42_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOA20417.1"
                     /translation="MEIKRGNKKFYVGEEETPLAELLFSVDNDVILAEGTEVSKELGG
                     KGVGKLLLKELVDWARKENKKIKPICPFVKAQMEKNVEYHDLLVDTRSKMKLGMVQMS
                     MKDNIEENLQKTLDYCDRAKEKGCDLLFFPEVQLTPFFPQYEKKNVENLVITEQDERL
                     KKILDKARQHHMYISPNIYLEKDDKRYDASLWINRKGEIEGISKMVHILQAEQFYEQD
                     YYHPSEDGFKVYNTEFGHIGIVICFDRHLPESIRACTLMGADLIIIPTANTKDEPLEM
                     FEWEIRVQAMQNQVFIAMCNRVGLEGDMDFAGESIIVHPSGDVIYKAGDEETLIVQEI
                     DLSEVSIWREKRPYLKLRRPDFYK"
     gene            complement(2228..2800)
                     /locus_tag="PKH42_08020"
     CDS             complement(2228..2800)
                     /locus_tag="PKH42_08020"
                     /inference="COORDINATES: protein motif:HMM:NF026229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4489 domain-containing protein"
                     /protein_id="HOA20418.1"
                     /translation="MEDRKGYFNHNSLRFDCNPCFDNKFHKQDIILECGFRPQDAFFE
                     IDDGQVEEGQEFVLDRLKIDTTCLNKPLVKIEFSSLIIFEAEDEEGPEHEVEVDLLFK
                     LIRICNGEKECLQEWQYLYEIDVENSIDELEVEMSQPFTVTFCDRPCSGCCEYIMEVK
                     GIDFEGEFDDLRVVKPNLSAIATGFCDNKF"
     gene            2980..3462
                     /locus_tag="PKH42_08025"
     CDS             2980..3462
                     /locus_tag="PKH42_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20419.1"
                     /translation="MNMYINPFENIYPCWSIFQSQNNNNMIENQNATGFMRLHIIDQV
                     TKENIPYATITAYVTDEARRDIPIMHLVTTLNPVRIELPIAHELGTKIQGPEYVFSTY
                     NISVDIFGYFTNIIYNNRLFPGVTTDFVIEMVPITVPQPVPIIEERLDIPPHPRDIVP
                     "
     gene            3787..4236
                     /locus_tag="PKH42_08030"
     CDS             3787..4236
                     /locus_tag="PKH42_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA20420.1"
                     /translation="MEEIGIGILFALLAGLLMSVQGVFNTRVMDSSSMWAATSWVHIS
                     AFAASISLWYFSGREALLSVFEVSEKIYLLGGVIGAFITYTVIKGISELGPAFATMFI
                     LLAQLVVSYLIEVFGLFGTEKVSFEWIKFIGVALMISGIVVFCKGGL"
     gene            complement(4247..6037)
                     /gene="glmS"
                     /locus_tag="PKH42_08035"
     CDS             complement(4247..6037)
                     /gene="glmS"
                     /locus_tag="PKH42_08035"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOA20421.1"
                     /translation="MCGIVGYIGYEDVKEIILGGLEKLEYRGYDSAGIAVLNNNEVYV
                     CKERGRISDLRKNIDSKITGNIGIGHTRWATHGAPNQINSHPHQSSSKRFTLVHNGII
                     ENEEELKQKYLSDIEFQSDTDTEVIVQLIEKFVNDGEEVELAIRHAMSKLIGSYALAI
                     IDKEDMNTLYAAKNKTPLLAGKGNGFNMLGSDAMAMINHTNLFYEIEDKEFLKIKKDD
                     IEIFTIYGNKVNRQPFKADIDASDTEKGTYPHYMMKEIEEQPFVIRKIMSEYSDESGN
                     MRISEEILQQIRDCDKIYIIACGTSYHAGLIGKQYFEKISHKPTEVIIASEFIYNMPL
                     LTEKPLFMFISQSGETADCRAVLVKVKEMGYKTLALTNVKGSTLSREADNTLLLYAGP
                     EIAVASTKAYTAQITVLAILAALVDNKINIDVFKELSKIAYIMESLCDNKDNYKKLAE
                     MYLVNSRNCFYIGRSMDYYLCLEAALKLKEISYIQTEGFPAGELKHGTIALIEKGTPV
                     IAVITQENINLNTRSNIKEVKARGAATMVISMHKISQERDHIVIDDAHELLSPLASII
                     PVQYLAYYAALIKGCDIDKPRNLAKSVTVE"
BASE COUNT         1939 a         1177 c          947 g         2392 t
ORIGIN      
        1 aatggagcct ggttctttta gtatcatagg atattttaat ttcagtgttt ggtaattttg
       61 acaattctaa aattgcctta taatctgtgg cacccatata agatgtagtt attattcgca
      121 atttgtgttc ctttgtaaat tcttttaatg cttcatatat ttgaactaac ccgctccatt
      181 taataaaaga taccagcata tctatcctat ctgctgtatt tatttcttta actaattcgc
      241 ttcctaatga tggttcattt tttgcacctg taaaaagtga attcattgct attgaagtaa
      301 taggtcttat cttattattt ttattttttt caattaatga attatcaatg caattcaaaa
      361 cagatagcaa taattctgca ttttcactaa taataaactc attgaaatca ttcaattcta
      421 actcggctac taaaagcata attattttat tgcatatttc aatttgctta ttcaatttat
      481 ctttatttgg aattctgttt agcgacttgt taattactat agacatgtat tttgataaga
      541 ttgaactgcc ttcttcttcg tctattttct ttgtttctgc atgatactct tttgtttgct
      601 taattttata tatttcttca ttaattagtt tattgataac gctttcataa aaaccattat
      661 taagtgacat tgccccacct ctgtctttaa tatgatcctt accctgtaac tgctataatt
      721 tatatcgaaa taattataac ataatatgac aaattaatcc agataatatt aacttttatg
      781 caaaaaaggc tctaaatctt taatttagaa cctttaatat ttatattttt ttgtataatg
      841 ccttattccg tatacttcta atatctctat ggtttttata actgtggaaa ttacttgtaa
      901 aaatctggtc ttcgaagttt taggtaaggc cttttttccc tccatataga tacttctgac
      961 aaatcaattt cttgaactat caaagtttct tcatcgcctg ctttatatat tacgtcaccg
     1021 ctgggatgca caataataga ttctccggca aaatccatgt ctccttccaa accaacccgg
     1081 ttgcacatgg ctatgaaaac ctgattttgc attgcctgga cacgaatttc ccattcaaac
     1141 atttccaatg gctcatcttt tgtattggca gtgggaataa taattaaatc agcacccatt
     1201 aaagtacatg ctcgtatgct ttccggtaag tgcctgtcaa aacatataac aattccaatg
     1261 tgaccaaatt cagtattata aaccttaaat ccatcttcag aaggatgata ataatcctgc
     1321 tcataaaact gctcggcttg taagatatgc accatttttg agatgccttc gatttcacct
     1381 tttcgattaa tccaaagtga agcatcatag cgcttgtcat ccttttctaa ataaatattg
     1441 ggtgaaatat acatgtgatg ttgccttgcc ttgtccaata ttttctttaa gcgctcatct
     1501 tgttcagtaa taacaaggtt ctcaacattt ttcttttcat attgaggaaa gaatggagtt
     1561 agttgaacct ccggaaagaa aagcaaatcg cagccttttt cttttgctct atcacagtag
     1621 tcaagagttt tttgcaagtt ttcttctatg ttgtctttca ttgacatttg taccattcca
     1681 agtttcattt ttgacctcgt atcaaccaac aaatcatgat attcaacgtt tttctccatt
     1741 tgagctttta caaacggaca aataggtttt atttttttgt tttcctttct tgcccaatca
     1801 actaattcct taagtaacag tttgccaaca cctttcccgc ctaattcttt cgatacctct
     1861 gttccttcgg ctaaaataac atcgttatca acagaaaata ataattctgc taatggagtt
     1921 tcctcttctc caacataaaa ttttttattc cctcttttta tttccatgaa ttttcctcca
     1981 aatttgttaa atcattataa catattttga aatataattg ttcttgattt taaatttctt
     2041 tatgcttatt tagaaagcgt taaatatttt cattggaatg ctactatacc aaagtttacg
     2101 aaaatattta ggactttata aaaaccataa aaaccttgcg atgtaataaa taacctatcc
     2161 tttggataac aaaaaaggtt ccaaatctaa aagatttaga acctttaatt tattctaggt
     2221 ttgttgttta gaatttatta tcgcaaaatc cagttgcaat agcacttaaa ttgggcttta
     2281 ctactctcaa atcgtcaaat tctccctcaa agtcaatacc ttttacttcc attatatatt
     2341 cacaacagcc tgaacatggt ctgtcacaga atgttaccgt aaatggctga ctcatttcta
     2401 cttcaagctc atcaatacta ttttcaacat cgatttcata cagatactgc cattcttgca
     2461 ggcattcttt ttcaccattg caaattctta ttaacttaaa cagcaaatct acttctactt
     2521 cgtgttcagg accttcttca tcttctgctt caaagatgat aagacttgaa aattcaattt
     2581 ttactagagg tttatttaag caagtggtat ctattttcaa gcgatccaga acaaattctt
     2641 gtccttcttc aacttggcca tcatcaattt caaaaaatgc atcttgtggt ctgaatccac
     2701 attccaaaat tatatcctgt ttatgaaatt tattatcaaa acatgggttg caatcaaatc
     2761 ttaaactgtt atggttaaag taaccttttc tatcttccat aaaactattc actccattag
     2821 gtatataaga attattttct ctgccttatt atatgtggct tatcccattt gtgttcatga
     2881 gaggaaaaaa atttcttaag cttttgtatg cttcttatca ttatacctgc atataatatt
     2941 gtaatactgt tttattgatt ttttaaggaa ggaaatatta tgaatatgta tataaaccca
     3001 tttgaaaata tatatccttg ctggagtatt tttcaaagtc agaataataa taatatgata
     3061 gaaaatcaaa atgccaccgg atttatgaga cttcatataa tagatcaagt gaccaaagaa
     3121 aatattccct atgcaacaat aacggcctat gtaactgatg aggcaaggag ggacattccg
     3181 attatgcatt tagtaacaac cttaaatccc gtaagaatag aattacctat agctcatgaa
     3241 ttaggaacta aaatacaagg acctgaatat gttttttcaa catataatat aagcgtagac
     3301 atttttggat actttactaa tattatttat aataatcgtt tattcccggg tgtaacaact
     3361 gattttgtaa tagaaatggt ccccattaca gtgcctcaac ctgttcccat tatagaggaa
     3421 agattagata tccctccgca tccaagagat atagtgccat aagaaataaa ttaaaaataa
     3481 actgatgaat taaaagatat ttttaattaa accaactttt ttaggaatac tataccatag
     3541 ttacaatcca aaaattttat tatgtaatct aaataaagtg aataaacgct ttgtcatcct
     3601 gagcgtaagc gaaggatctc aagagattct tcgggctaaa gccctcagaa tgacaagaat
     3661 gagattcttc actgcattca gaatgacgag aatgagatta ttcacatgcg ttcagaatga
     3721 caaaaacgag attcttcact gcgttcagaa tgacaaaact cagaatgaaa ctatagaaaa
     3781 attatattgg aggagattgg tataggtatt ttatttgcac ttcttgcggg gctgttaatg
     3841 agtgttcaag gagtttttaa tacacgtgta atggattcat cgagtatgtg ggctgcaacc
     3901 agctgggttc atatatcagc atttgctgca agtataagtt tatggtattt ttcgggacgg
     3961 gaagcattgc tttcggtatt tgaagtgagt gaaaaaattt acttacttgg cggcgtcata
     4021 ggtgctttta ttacatatac cgttattaaa ggaatttcag aactaggacc tgcatttgca
     4081 acaatgttca tccttttggc tcagcttgtt gtatcttatt tgattgaagt tttcggattg
     4141 tttggtaccg aaaaggttag tttcgaatgg ataaaattta tcggtgtagc tctgatgatt
     4201 tccggaatag ttgtattttg caagggcggt ctttgaccgc ccttaattat tcaacggtaa
     4261 ccgattttgc caaattcctt ggcttatcaa tatcgcatcc ttttatcaag gcagcataat
     4321 aagccaagta ttgaacaggg attattgatg ccaaaggtga cagcagttca tgtgcatcat
     4381 ctattactat gtggtctctt tcctgactga ttttatgcat ggatataacc atggtggcag
     4441 ctcctcttgc tttaacttcc ttaatgttgc ttcttgtatt gagatttata ttttcttgtg
     4501 taattactgc aattacggga gtaccctttt caattaaagc tattgttcca tgctttaatt
     4561 ctcctgccgg aaagccttca gtttggatat aagaaatttc ttttagcttg agagctgctt
     4621 caaggcatag atagtaatcc atgcttcttc ctatatagaa gcaatttctt gagtttacaa
     4681 gatacatttc ggctaatttt ttgtaattat ccttattatc acaaagagat tccattatat
     4741 atgcaatttt ggacaattct ttaaaaacat caatattgat tttgttgtca accaaagctg
     4801 caagtatcgc taatactgta atttgagcag tgtatgcctt tgtggatgca acagctattt
     4861 cgggtcccgc atataacaaa agcgtgttgt ctgcttctct tgataatgtt gagcctttca
     4921 cattggtcaa tgcaagagtc ttataaccca tttcctttac ttttaccaac acagccctgc
     4981 agtcagcagt ttcgccgctt tgtgaaataa acataaacag cggcttttcg gtaagaagcg
     5041 gcatgttgta aataaattcg cttgctatta ttacttctgt gggcttatga gaaatttttt
     5101 caaagtactg ttttccgata agtccggcat ggtagcttgt gccgcatgca ataatgtata
     5161 ttttgtcaca atctcttatt tgttgtaaaa tttcctcgga aattctcata tttccacttt
     5221 cgtcggagta ttctgacata attttcctta ttacaaatgg ttgttcttct atttccttca
     5281 tcatgtagtg aggatatgtc cccttttcag tatccgaagc atctatatcc gccttgaagg
     5341 gctgcctatt tactttgttg ccatatatcg taaatatttc tatgtcgtct tttttgattt
     5401 tcaaaaattc tttatcctcg atttcataaa ataaatttgt atggtttatc attgccatgg
     5461 catcacttcc aagcatgtta aaaccgtttc ctttaccggc taacaaaggt gttttattct
     5521 ttgctgcata gagtgtgttc atatcctctt tatctattat tgcaagggcg taagaaccta
     5581 tcaatttgct catggcatgc cttatggcaa gttcaacttc ttcgccgtca ttaacaaatt
     5641 tctctataag ctgaacaata acttctgtat ccgtgtcact ttgaaattca atatcactta
     5701 aatatttttg ttttaattct tcttcatttt caataatccc attgtgtaca agggtaaatc
     5761 tcttagagct gctctggtga ggatgtgaat ttatttggtt gggagccccg tgagttgccc
     5821 accttgtatg cccgattcct atatttccgg taatttttga gtctatattt tttcttaagt
     5881 ctgaaatcct tcctctttct ttgcatacat atacttcatt gttatttaat acagcaatac
     5941 ctgctgaatc gtatcctctg tattcaagct tttcgagtcc tcccaaaatt atttccttaa
     6001 catcttcata tccgatatag cctactattc cgcacataat atttctccat tctttttaat
     6061 ttttttaata gcaaataaca cctgaaaaat tttttcaagc aaagaaatcc ttcacctgca
     6121 gtaagaataa caaaagagat tcttcactgc gttcagaatg actaaaaagt ttattcgttg
     6181 caataacaaa aatggggaaa ctaagactat tatttcagta agatttgaag ctgtcgattt
     6241 tattttgtca ttacagtcgc ctgtaatatt tgtaaaaatc atatcggtgc agctacaccg
     6301 cagagcaccc gccgaatttt cgaaaactct gtcctcgtca acttaaaaaa gttctggcgc
     6361 tataataatt ttgtttccca aactacataa taccagatta tgtttatact aaattttagt
     6421 tactacattt agtattttat cacaaaatca attaa
//
LOCUS       DAOPUE010000097         6423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12009_length_6423_cov_10.941269, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000097 DAOPUE010000000
VERSION     DAOPUE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12009_length_6423_cov_10.941269"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1779)
                     /locus_tag="PKH42_08040"
     CDS             complement(52..1779)
                     /locus_tag="PKH42_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA20422.1"
                     /translation="MNILMNIKHELQHIIEAMHAVTNVDITIVDESLQRIATTVNEEY
                     SLGAKAPIYSAFHKCIMTGEQYFLENPKENPICFDCENVQSCKELVELCIPIKFNGKI
                     LGVIGMCAYDEKARQNLLMNRESFMNLESQLVNIISTVISEKKIMKMLEYSSSELMTL
                     INSLNEGIIIINNNYEIINTNNYANEKLNINTSKIPDLWNLLSEKLYNNLISKNFIGE
                     VGPVKLNNTEFVINAYPIMVKDEREGTVLVLSDFNKMKESVIESNRKKDIVTFDDIVG
                     ESEALKQARREAIQVADSDASILILGETGTGKEVFARAIHNLSYRKNEVFMAINCGAI
                     PENLIESELFGYEKGSFTGASVTGNPGKFEICKDGTLFLDEIGELPLSMQVKLNRALE
                     EKEILRVGGNNPIKVNPRIISATHCSLASMVENNLFRDDLYYRLNVVPIHLPPLRERG
                     YDVIILSRYFLNRFNNVYKKQLQGFTPEAERLLMGYKFPGNIRELRNLIEYAVIFEEN
                     SYIGAENLRKKMGTSTSENLKLSELTRLYEKSVINSKIQELGGDLESKKKAAEHLGIS
                     IATLYRKLE"
     gene            2135..3400
                     /locus_tag="PKH42_08045"
     CDS             2135..3400
                     /locus_tag="PKH42_08045"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOA20423.1"
                     /translation="MKTNLLTNILKDQVTPALGCTEPGAVAYAVARAKEILDSEVKKL
                     NIEVDKNILKNGFSVGIPGTEEHGIIFAAALSLVIGKSEYALEVLKDVDKESIDRALE
                     IVDSNIIDLRLNSHVQGLYIKAEAIGDKDSATVIIRDAHTNIIFERKNDLVLFDRGDI
                     TEKKANNIKFEIKNFTIDELIDYVNNVPIENISFIQNGIDMNLRIADAGLKETPGVGL
                     SNYLYNKAGDVFTMAKAYTAAASEARMSGYPLPVMSSAGSGNHGLVAVIPISYIGREN
                     KISNERILRSVTLSHLITIFVKVYLGALSPVCGCGVAAGVGCAAGLTYMLGGDNEQIK
                     GSINNMIAGISGMLCDGAKLGCAYKLSISVDAAVDAAKMALEKIYIPEDNGILGCTAE
                     KTIQNLAQVSNIGMDHTDEVILDVIINKC"
     gene            3486..4853
                     /locus_tag="PKH42_08050"
     CDS             3486..4853
                     /locus_tag="PKH42_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOA20424.1"
                     /translation="MKKRISLLIVILMVILSFTSALAIEIDEDKFDQDIEFMKKVIYF
                     VLDKYQYEVSQEDIINGLYDGFFSVLDDYSTYYTPKEYQALLEDTAGEFSGIGVQIMD
                     INGQVVVVTPLPGSPAIEAGIKAGDIIKTVDHYDITGVTSSQASLLIRGEEGTPVKIG
                     ILRGKENLTFNLIRRAIVINNVEGKILEDNIGYLKVTGFSDNTAELVEKELELFDEKN
                     VKKIIIDMRNNGGGTLQSAVDLLNLFVTEGPVVYVKYAAGKEDIYESKLKDKKYEIAV
                     LINEGSASATEIFAGAVKYKNEGIIIGTNSYGKGVVQSLYHLTDGSGVKFTTAEYFSV
                     DRIPVHKIGITPDIAVENEKTDISEYPKFSKEQKPELGNVSLDVLAAEMILMELGYEI
                     NQPDGVYDIQSFEQVRKFQEDNLLYPYGTIDFATQDALYNALIKHMESNLEDLQLKAA
                     IEALK"
     gene            complement(4919..5470)
                     /gene="yyaC"
                     /locus_tag="PKH42_08055"
     CDS             complement(4919..5470)
                     /gene="yyaC"
                     /locus_tag="PKH42_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOA20425.1"
                     /translation="MNPIEVHYKNSFAKIKIGNFLAEHMNNAAIVCIGTDKCIIDSLG
                     PLTGTLLMKENIKIDVYGTLEKPVHAMNINEYIKNLRSKNYDCIIAVDACLSNKKSQG
                     IIEVIEGSITPGKGIGKFLPEIGDISIIGIVNSSDKEFHDLVQDTRLSLIYEMAETIC
                     GGILNACSMNISESFDQASISSV"
     gene            complement(5522..6265)
                     /locus_tag="PKH42_08060"
     CDS             complement(5522..6265)
                     /locus_tag="PKH42_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOA20426.1"
                     /translation="MWERAELKARAKQVLKENYWKSFLISLVLLLSGADSRGGGSSGR
                     GAGERIGEWIDYSEYLIIIIAVVILVIAFRVLIGYALEIGSRKFFVQSAQYKNDKGCY
                     NFAFDTGNFKGIISTMFLRDIYNFLWTLLLIIPGIIKSYSYRMVPYILADNPNFGADN
                     AITLSRKMMDGNKFALFVLELSFLGWFLLGLLAFGVGILFVNPYVYATEAQLYLVLRD
                     TAIDQGYCTAEDLNLYNDFVKHDGYYWEE"
BASE COUNT         2119 a         1151 c         1096 g         2057 t
ORIGIN      
        1 tttaacctat aatctaatca aagtcaaaaa cttatttcaa gctttacgta attactccaa
       61 ctttctgtac aaagtggcaa tacttatacc aagatgctcg gcagcctttt tctttgattc
      121 taaatcaccg cctaattctt gaattttact gtttatgaca gatttttcgt acagccttgt
      181 caattccgac aattttaagt tttcggaagt gctggttccc attttttttc tgagattttc
      241 tgctcctata tagctgtttt cctcaaatat taccgcatat tcaattaaat ttctaagctc
      301 tcttatgttt ccgggaaact tgtatcccat caataaccgc tctgcttcag gcgtaaaccc
      361 ttgcagctgc tttttataga cattgttaaa gcggttaaga aaataccttg ataaaataat
      421 cacgtcatat cctctttctc tcaaaggagg aagatgaatg ggaactacat taagtctgta
      481 gtataaatca tctctgaata gattattttc aaccatagat gccaaggagc agtgagtagc
      541 agaaattatg cggggattaa cctttattgg attattacct cccactctta atatttcttt
      601 ttcttcaagg gccctgttta attttacttg cattgataaa ggaagctcac ctatttcatc
      661 caaaaacaat gtaccatcct tgcaaatttc aaatttgccc ggattgccgg ttacactggc
      721 acctgtaaat gagccctttt cgtatccgaa aagctcgctt tcaattaaat tttcaggaat
      781 tgctccacag tttatagcca tgaacacttc attttttcta tagcttaaat tatgtattgc
      841 tcgagcaaaa acttcctttc ctgtacctgt ttcaccgagt attaaaatag aagcatcgct
      901 gtcggccact tgtattgctt ctctccttgc ctgtttcaag gcctcgcttt cgcctacaat
      961 atcatcaaat gtaactatgt ctttcttcct gttggattca attaccgatt ccttcatttt
     1021 gttaaagtca gacaatacca atactgttcc ttccctttca tcctttacca tgatggggta
     1081 ggcatttatt acgaattcag tgttattaag cttgacagga cccacttctc caataaaatt
     1141 cttggaaatt aaattattat aaagtttttc tgatagcagg ttccacaaat cgggaatttt
     1201 tgaggtgtta atatttaatt tttcgttagc ataattgttg gtatttataa tttcataatt
     1261 attatttata ataataattc cttcatttaa tgaattaatc agcgtcatca attcagatga
     1321 tgaatattcc agcatcttca taatcttctt ttcactgata acggtagata ttatattaac
     1381 taattggctt tctaaattca tgaagctttc acgattcatc aacaaatttt gtcttgcttt
     1441 ctcatcataa gcacacatgc cgattacacc taatattttt ccgttaaact taatgggaat
     1501 gcatagctcg actaattcct tgcagctttg aacattttca caatcaaaac atatggggtt
     1561 ttccttgggg ttttccagaa aatattgctc accggtcata atgcatttgt ggaaggcaga
     1621 ataaatagga gcctttgctc caaggctgta ttcctcatta acagtggttg caatcctctg
     1681 aagagattca tcaactatgg tgatatctac atttgttact gcatgcattg cttcaataat
     1741 atgctgaagt tcatgcttaa tattcattaa aatattcatt taacctctac cccgctcgaa
     1801 ggatatcttc ttcattataa tatattataa tacaagatgc ttattaatac tatactttaa
     1861 tttgaaattt tcaaaatatt tatcattttt gataattctt gcgcaaaact gataaaacgt
     1921 ttgtaagatt caaattatgc aaacttataa attcctttat aatttattta tcatttttga
     1981 taattttctt agatcattct ttgactaaag gtctattttt caatcaattc ttaaaattag
     2041 gcattggcat gattcatgca tataaattat taaagacgag aaactcactt gtacttgcag
     2101 tatcaggtta aataaaataa aagaggagat gcatatgaag actaatctat tgacaaatat
     2161 attgaaagat caggttactc ccgcattagg atgcactgag cccggagcag ttgcttatgc
     2221 ggttgcaaga gcaaaagaaa tattagactc cgaagttaaa aagcttaata tagaggttga
     2281 taaaaacata ttaaaaaacg gattcagcgt aggtatcccg gggacagagg agcacggtat
     2341 aatatttgca gctgccctct cattagtaat aggcaaatca gaatatgcac tggaagttct
     2401 gaaagatgtc gataaggaaa gcatcgatag ggcattggaa atagttgatt caaatataat
     2461 agacctaaga ctcaattccc atgttcaagg attgtatata aaagcagaag caatcgggga
     2521 taaagatagt gctacggtta ttattcggga tgctcatacg aatattattt ttgaaagaaa
     2581 aaatgatctt gttttgtttg acaggggtga tattaccgaa aagaaagcaa ataacataaa
     2641 atttgaaata aaaaacttta ctattgatga gcttattgat tatgtaaata atgttcccat
     2701 agaaaatatt tcttttattc aaaacggaat cgatatgaat ttaagaattg ccgatgcagg
     2761 attaaaagaa actcccggtg tcggtttgag caattatctg tataataaag caggggatgt
     2821 ttttacaatg gctaaggcat atactgctgc cgcttcggaa gcaagaatgt ccggataccc
     2881 tttaccggta atgagcagtg ccggttcagg caatcacggt ttggtagcag taattcccat
     2941 atcatatata ggtcgggaaa ataaaatttc aaacgaaaga attttgcgct ctgtaacctt
     3001 aagtcatttg attacaattt ttgtaaaagt atatttagga gcactttcgc cggtttgcgg
     3061 ctgcggagtt gcagcaggag ttggttgtgc agcaggtctc acatacatgt taggaggaga
     3121 caatgagcag attaaaggct caataaacaa tatgatagcg ggaatttccg gcatgctctg
     3181 tgacggggca aagttaggct gcgcctacaa actgtcaata tcagttgatg ccgctgttga
     3241 tgctgcaaaa atggccttgg aaaaaatata tattccggaa gataacggta tcttaggatg
     3301 tacagcagaa aaaacaattc aaaatttagc acaggtttca aatataggca tggaccatac
     3361 agacgaagtt atacttgatg ttataattaa taagtgctaa cataggtatg tccactcata
     3421 gttaagaaac atattcgttc gcggtgtgtc caccctatga taaatctaaa gaaacgaggt
     3481 gctttatgaa aaaaagaatc agcttgttaa ttgtaatttt aatggtaatt ttatcattta
     3541 cttctgcatt ggcaatagaa atcgatgaag ataaatttga ccaagatatt gaatttatga
     3601 aaaaagtaat atattttgtg cttgacaaat atcagtacga agtaagccag gaagatatta
     3661 taaatggtct ttatgacggg tttttcagtg ttttggatga ctacagcact tactatacac
     3721 ccaaggaata ccaggctctg cttgaagaca cagcaggaga attttcaggc ataggcgttc
     3781 aaataatgga tataaacgga caagttgtgg tagtaactcc tctgcccggc tcacctgcaa
     3841 ttgaagcagg aattaaagca ggtgatataa taaagactgt tgaccattat gacattaccg
     3901 gagttacttc aagtcaggca tctcttctta taaggggaga agaaggtact cctgttaaaa
     3961 ttggaatttt acgcggaaag gaaaatctga cctttaattt aataaggagg gctatagtaa
     4021 taaataatgt tgaaggaaaa atattggaag ataatatagg atatttaaaa gttaccggtt
     4081 tttccgacaa cacagcggaa cttgtagaaa aagaattaga attatttgat gaaaaaaatg
     4141 taaaaaaaat tataatagat atgcgtaaca atggcggcgg aacattgcag tctgcagttg
     4201 acttgttgaa tttatttgtt acggaaggac ctgtagtata tgtaaaatat gcagccggca
     4261 aggaagatat ttatgaaagc aaacttaaag ataaaaaata tgaaattgcc gtattgataa
     4321 acgaaggaag tgccagcgca acagaaatat ttgcaggggc agtaaagtat aaaaatgaag
     4381 gaattattat aggaacaaac tcttacggaa aaggagttgt tcagtctctg tatcatttaa
     4441 ccgatggaag cggagtaaaa tttaccacag cggaatattt ttcagtagat agaatacccg
     4501 tacataaaat aggaataaca ccggatattg cagtagaaaa cgaaaaaacc gatatttctg
     4561 aatatccgaa gttcagcaag gaacaaaaac ccgagttggg aaatgtcagt cttgatgtat
     4621 tagctgctga aatgatactt atggaattag gttatgaaat aaaccaaccg gatggagttt
     4681 acgacataca atcatttgaa caagttagaa aattccagga agacaattta ttatacccat
     4741 acggaacaat tgattttgct actcaggatg cattgtacaa tgcattgata aaacacatgg
     4801 aaagcaattt agaagattta cagctgaagg ctgccatcga agcattaaaa taacaaaaac
     4861 tggggacatt cctcttactt caaagacttt ccctaaagaa gaggtgtgtc cccatacctt
     4921 atacactgct tatcgaagcc tggtcaaaac tttctgatat attcatgctg caagcattta
     4981 atattcctcc gcagatcgtt tctgccatct cataaataag agaaagtctt gtatcctgaa
     5041 ccaaatcgtg aaactcctta tcgctggaat ttacaatccc tattatagat atatccccga
     5101 tttcaggaag gaatttccct ataccctttc ccggtgtgat tgagccctca ataacttcta
     5161 taattccttg acttttctta ttgcttaagc aggcatccac cgctattatg cagtcataat
     5221 tcttgcttct taagtttttt atatactcgt taatattcat tgcgtgaaca ggtttttcca
     5281 aagttccgta aacatcaatt tttatatttt ctttcatcag taaagtacct gttaagggtc
     5341 ctaagctgtc tattatgcat ttatccgttc ctatacatac tattgctgca ttattcatat
     5401 gctctgccaa aaaatttcct attttaattt ttgcaaagct gttcttataa tgtacttcaa
     5461 ttggattcaa aggaaacacc ccctatgtgt tagtatatac acacaggggg tgttttatgc
     5521 tttattcttc ccaataataa ccatcatgtt ttacaaaatc attataaaga tttaaatctt
     5581 cggcagtgca gtatccttgg tctatggctg tatccctgag taccagataa agctgagcct
     5641 cagttgcata gacataagga tttacaaata gaattccaac tccaaaagct aacaaaccga
     5701 gtaagaacca accaagaaaa gacaattcca agacaaataa tgcaaattta tttccgtcca
     5761 tcattttcct gcttaaggtt atggcattat ctgcaccaaa attaggattg tcagccaaaa
     5821 tgtagggaac cattcgatat gaataagatt ttattattcc cggtattatc aataacaatg
     5881 tccaaagaaa gttatatata tcccttaaaa acattgttga aattatacct ttaaaattcc
     5941 ctgtatcaaa agcgaaatta tagcagccct tatcgttctt atattgagcc gactgtacaa
     6001 agaattttct tgagcctatt tccaatgcat aacctattaa cactctgaaa gcaattacaa
     6061 gaattacaac ggcaataata attatcaaat attcagaata atcaatccat tcgccgattc
     6121 tttcccctgc acctctgccg gatgaccctc cgccgcggct atctgcacct gataatagta
     6181 aaactaaact aattaaaaat gacttccaat aattttcctt taatacttgt tttgctctag
     6241 ccttaagctc tgccctttcc cacatattcc ctccttgtta attttttaaa ttaattatat
     6301 taaatttctg acaaggggac gggggtattg tcatatacac aaattcccta gcaaagatga
     6361 cagcaccccc gtccccttgt cacctatata taaaatgaca aggcggtata taccaacaac
     6421 agt
//
LOCUS       DAOPUE010000098         6354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12178_length_6354_cov_12.375774, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000098 DAOPUE010000000
VERSION     DAOPUE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12178_length_6354_cov_12.375774"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PKH42_08065"
     CDS             <1..447
                     /locus_tag="PKH42_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA20427.1"
                     /translation="GNEFLEKAVICKVDEKFNPIEGTEITFDVDVMCISVGLTPLNEL
                     LAMRGCEMKYIPQLSGLVPVRNENYETTVENVFVAGDATGVEEASAAMVEGFLAGLCA
                     AAKIGYKPDDFMERKNDYEEQLNNLRSGPAGKHILAGIEQIRRSYV"
     gene            440..928
                     /locus_tag="PKH42_08070"
     CDS             440..928
                     /locus_tag="PKH42_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOA20428.1"
                     /translation="MFEKTGIPSTEMIIKKFPPIDRINKGAVAVIECYKEIPCNPCET
                     ACKFDAIKVGEDINNIPEARFENCTGCAICLSKCPGLAIMIVDGSKSESTVEVKIPYE
                     FLPLPKEGQIVKGLNREGNYITDVKVLKILNPKSFDRTPVITIEVDRKFLYEIRNIRV
                     EG"
     gene            930..1211
                     /locus_tag="PKH42_08075"
     CDS             930..1211
                     /locus_tag="PKH42_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOA20429.1"
                     /translation="MDENTIICRCSDVTLKEIRELIKDGYVTFDEIKRITRAGMGPCQ
                     GKTCGQLIMREIANITGQNMKDIKFQTNRPPVVGVKLGLIAEEARKNEE"
     gene            1201..2349
                     /locus_tag="PKH42_08080"
     CDS             1201..2349
                     /locus_tag="PKH42_08080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOA20430.1"
                     /translation="MKNKAEVVIIGGGISGCSIAYNLAKKGIKDIVVLEKSYICSGST
                     GRCGAGVRMQWGTEMNCKIAKKSIEFYENANEILEYERDVEFKQKGYLLVADTEHEVE
                     QFKKNIEVQHSCGIPSTMLSLKEAKEIVPYLNTDILKGAAYCEKDGFLNPFHTTDAFY
                     RAANRLGAKFYTFTEATGIKVEKGRVTGVETNKGFISTNIVVNATNGYSKSICNMVGL
                     DVPIYSERHQILVTEPVEPMQDPMVMSFGLNFYIQQSPEGTFIMGRGDENEPKDLRVT
                     SSWQFLEEMTKTIDVVLPPISKLRVIRQWAGLYNMTPDKQPIYDEAESAKGFYIALGF
                     SGHGFMFGPITGIAMSEMILKEKPTIDVSMLNLNRFKTGKMLLEPSVV"
     gene            2515..3603
                     /locus_tag="PKH42_08085"
     CDS             2515..3603
                     /locus_tag="PKH42_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOA20431.1"
                     /translation="MRIKYLNKLVEILKKKEIDAILIAPSEEMEFIMGHNTHLCERFQ
                     ALVIKSDGNYFYICNLLTVDEIQSVLGPNIKVYGWFDGDVFTDTVKKAFEEQGLIGKT
                     IAVNSTERAFIILQILEAMDVKFINGKTVLEDMRIIKDEEELNNLRMAARITDESYEE
                     LLKFIKPGIKEADIARKMNEIFKEKGADEGFTMVCSGPNSSYPHYNDDQRVIQEKDII
                     VLDWGCKYNNMCADMSRTVFVGGITEEERKVYEIVKASQEAGEKAAVKGAYIPDVDKA
                     ARDIIEKAGYGKYFFTRLGHGIGYSVHEAPDIKASNKRNLEKGMVFSIEPGIYMAGNF
                     GIRIENIVAVTDDGNEILNKSTKEIMII"
     gene            3659..4687
                     /locus_tag="PKH42_08090"
     CDS             3659..4687
                     /locus_tag="PKH42_08090"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOA20432.1"
                     /translation="MDYKELALELHKNNIVVDSHLDLAGEIYNRNRAGEKEIIKNYYL
                     ESFKKGGFNLIISSLYIDDLFLPEMALRIALGQIRALIEDVESCHGEVMLVKSREDLN
                     KAVNEDKIGIIISFEGLEPIGSDIGLLRIFYELGVRAAGLVWSRRNYVADGCSFFPVE
                     EGQRGGLTRFGVKVVNKMEEMNMLIDVSHLNDEGFLDVLKYTKKPFIASHSNSRTIHG
                     SMRNLSDEHIRAIADRKGVIGINAIKSIAGVKDGEDPIRKLADHIEYIVNLAGAHHVG
                     YGFDLCNGYYKSELKYKFATNNSDSLSSHDEAVLLTEELLRRGISKEDCILIIGGNFL
                     RIFNNILA"
     gene            4701..>5197
                     /gene="lepB"
                     /locus_tag="PKH42_08095"
     CDS             4701..>5197
                     /gene="lepB"
                     /locus_tag="PKH42_08095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOA20433.1"
                     /translation="MTGLDKWQYFIKEWVFPVVIALIIVLFLNKFVFILVTVPTGSME
                     DTILPGDRLYINELFDINDAKRGDILVFKSDELEDKRLVKRLIGLPGETVEIKRDGSI
                     YVDGEKIEEPYAINFRGEDKVFIVPEKSYFFLGDNRPISYDARYWNEPYISEDKVIGK
                     VEFRF"
     assembly_gap    5198..5297
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5298..>6354
                     /gene="pepD"
                     /locus_tag="PKH42_08100"
     CDS             <5298..>6354
                     /gene="pepD"
                     /locus_tag="PKH42_08100"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HOA20434.1"
                     /translation="NVIIKKPASRGYENAPVVMLQAHMDMVCEKNEGVEHDFDKDPIK
                     LRIEDGYIYAADTTLGADNGIGVAFALSVLDSELEHPSLEVLITTDEEKGMTGADKLD
                     GSLFKSKYLLNLDSEEEGVFTAGCAGGSEIDFKIPVKYQNTKGRAYKISVKGLSGGHS
                     GVDIHKEKGNANKILGRILYDLIDDVELASIEGGAKTNAIPREANAIVTIKSDKVPEK
                     IAKWNKILKNEYAFTDPSIRVALTDLNEEVFPLEKEIFKKTVALINLIPVGVLSRSTA
                     IDLVISSNNLGVIRTDEKYITLFNHPRSSVESLLTEDFIPAMKQLASILDVEYVLGSY
                     YPGWEYAKESKIRDICIE"
BASE COUNT         2247 a          844 c         1447 g         1716 t
ORIGIN      
        1 ggcaatgagt ttttagaaaa ggctgttata tgtaaggttg acgaaaaatt taatcccata
       61 gagggaacgg aaattacttt tgatgttgat gtaatgtgta tctccgtagg tttgactccg
      121 ctaaacgaac ttcttgccat gagaggttgc gagatgaagt atatccctca gttgagcggt
      181 ctggttccgg taagaaatga aaattatgaa actactgttg aaaatgtatt tgttgcagga
      241 gatgctaccg gagtggaaga agcaagtgct gcaatggttg aaggatttct ggcaggtttg
      301 tgcgctgcag caaaaatagg atataagcct gatgatttca tggaaagaaa aaatgattat
      361 gaagaacaat taaataattt acgctcaggt ccggcaggaa agcatatatt agcaggcatt
      421 gagcagataa ggaggtcata tgtttgaaaa aacaggaatt ccaagcacag aaatgattat
      481 taaaaagttt cctccaatag acaggattaa caaaggtgca gttgcagtaa tagagtgcta
      541 caaggaaata ccctgcaatc cttgtgaaac tgcatgtaaa ttcgatgcaa taaaagtagg
      601 ggaagatata aacaatattc ccgaagctag atttgaaaac tgcacgggct gcgcaatatg
      661 cttaagcaaa tgtccgggac tggcaattat gatagtggac ggctcgaagt cagagagtac
      721 ggttgaagta aaaataccct atgaattttt gcctctgccg aaagaaggac aaattgtaaa
      781 aggacttaac agggaaggaa attatattac ggatgttaag gttttaaaaa tattaaaccc
      841 gaaatctttt gacagaacac cggtaattac aatagaagtt gacagaaagt ttttatacga
      901 aataagaaat atcagggtgg agggataata tggacgaaaa tactataatc tgcagatgct
      961 cggatgttac attaaaggaa atcagagagc ttatcaaaga tggttatgtt acgtttgatg
     1021 aaataaaaag aattaccaga gcgggcatgg gaccctgcca gggcaagaca tgcggtcagt
     1081 tgataatgag ggaaattgca aatattacag gtcaaaatat gaaagatata aaattccaaa
     1141 ccaacaggcc tcctgttgtg ggagttaaat taggactaat tgctgaggag gcaagaaaaa
     1201 atgaagaata aagctgaagt tgtaataatc ggaggtggaa tttccggttg ttccattgct
     1261 tataatttag ctaaaaaggg aattaaggat attgttgttt tggagaaaag ctacatatgc
     1321 agcggctcca caggaagatg cggtgccggt gtaaggatgc agtggggaac agaaatgaac
     1381 tgtaaaattg caaaaaaaag cattgaattt tatgaaaatg caaatgagat tcttgagtat
     1441 gaaagagatg tggaatttaa gcaaaaagga tatttgttgg ttgcagatac agaacatgag
     1501 gtggaacagt tcaagaaaaa tattgaagtt cagcattcgt gcggaatacc ttcaaccatg
     1561 ctctccttaa aagaagcaaa ggaaattgta ccatatttaa atacggatat attaaaaggg
     1621 gcagcctatt gcgaaaagga tggtttttta aatccgtttc atacaacaga tgccttttac
     1681 agagctgcta atagattagg cgctaaattc tatactttta cggaagcaac cgggattaaa
     1741 gtcgaaaaag gcagggtaac cggagtggaa acaaataaag gttttatatc tacgaatatt
     1801 gttgtaaatg ctaccaacgg ctacagtaag agtatctgca atatggtagg tcttgatgtg
     1861 cctatatatt ctgaaaggca tcagattctt gtgacagagc cggttgagcc tatgcaagac
     1921 cccatggtta tgtcattcgg actgaatttt tacattcagc agtcaccgga aggaaccttt
     1981 attatgggca gaggtgacga gaacgagcct aaggatttaa gagttacctc cagctggcag
     2041 ttcttggagg aaatgacgaa aacaatagat gtggttttac ctccaatatc aaaattgaga
     2101 gtaatcaggc aatgggcagg tctttacaat atgactcccg acaaacaacc aatttatgat
     2161 gaagcagaaa gtgcaaaagg attttacatt gcgcttggat ttagcgggca tggtttcatg
     2221 tttggaccta ttacgggtat agcaatgtct gaaatgatac tgaaggaaaa acctactatc
     2281 gatgttagca tgcttaattt aaatcgtttt aaaaccggaa agatgctttt agagccgtcg
     2341 gtggtttaat atcgccttat tgaatgtcca caaattcgat tgctttttat tttaaacaga
     2401 cgtataatta gataataaga tggtcatttt gaacaaaggt ttttgtcatc ctaagcatat
     2461 gcgaaagttg ctcaaagaat gacaatttac aatgacaaat tatgggagga tattgtgaga
     2521 ataaaatatt taaacaagtt ggttgaaata ttaaagaaga aagaaatcga tgctatatta
     2581 attgctcctt cggaagaaat ggaattcata atggggcaca atactcatct ttgtgaaagg
     2641 tttcaagcac tggttataaa aagtgacgga aattactttt acatttgtaa tttgctgacg
     2701 gtagatgaaa ttcagagtgt tttgggacca aatataaaag tctacggctg gtttgacgga
     2761 gatgtgttta cggatacggt taaaaaggca tttgaagagc aaggattgat tgggaagact
     2821 atagcagtaa attctaccga aagagcattt ataattcttc agatattgga agctatggat
     2881 gttaaattta taaacggcaa gactgtattg gaagatatga ggattataaa ggatgaagaa
     2941 gagcttaaca atttaagaat ggctgcacgt attacagatg agtcctatga agaattgttg
     3001 aaatttataa aaccgggaat aaaggaagct gacatagcca ggaaaatgaa tgaaatattc
     3061 aaagaaaaag gtgctgatga agggtttaca atggtctgtt caggaccaaa ttcatcttac
     3121 ccccactaca acgatgacca gagggtaatt caagaaaaag atattattgt gcttgactgg
     3181 ggatgtaaat acaataatat gtgtgccgat atgtcaagaa ccgtattcgt tggaggaatt
     3241 accgaagaag agcgaaaggt ttatgaaata gtaaaggcaa gccaggaagc aggggaaaag
     3301 gcagctgtaa aaggtgcata cataccggat gtggataagg ctgcaagaga tataatcgaa
     3361 aaagcaggtt atggaaagta cttttttaca agattaggac atggaatagg atattccgta
     3421 catgaagctc cggacattaa agcaagcaat aaaaggaatt tagaaaaagg catggtgttc
     3481 agcatcgagc cgggtattta tatggccgga aatttcggaa tacgtataga aaatattgta
     3541 gcagtaaccg acgatggaaa tgaaatatta aataaatcca caaaagaaat aatgataata
     3601 taaattattg tacttaaata ctgtctgtca ttttgcatta tttacaagag gagagcaaat
     3661 ggattacaaa gagcttgctt tagaattgca caaaaataat attgttgtgg attctcattt
     3721 agacctggca ggggaaattt acaacagaaa cagggccggg gaaaaggaaa taattaaaaa
     3781 ttactatctt gaaagtttta aaaaaggcgg ctttaattta attatatcat ctttatatat
     3841 tgatgactta tttttgcctg aaatggcttt aaggattgca ttgggacaga taagagcatt
     3901 aattgaggat gtggaatctt gccatggcga agtaatgctg gttaaaagca gggaagactt
     3961 aaacaaggca gtaaatgaag acaaaatcgg tataattatc tccttcgaag gattggagcc
     4021 gataggaagc gatatcggtc ttttaaggat attttatgag cttggagtaa gggctgcagg
     4081 attggtgtgg agcaggagaa attatgttgc cgacggttgc agttttttcc cggttgaaga
     4141 aggtcaaaga ggagggctta caaggtttgg ggtcaaggtt gtcaataaaa tggaagaaat
     4201 gaatatgctt attgatgtca gccacctaaa cgacgaaggt ttcctggatg tactcaaata
     4261 caccaaaaag ccttttatag cttctcattc gaactcaaga acaattcatg gaagtatgag
     4321 gaatttgtcg gacgaacata tcagggctat agcagataga aaaggtgtta taggaataaa
     4381 tgcaatcaaa agtattgcag gagtaaaaga tggtgaagac cctattcgta aattggcaga
     4441 ccacattgaa tatatagtaa atttggcggg ggcgcaccat gtaggttacg gttttgacct
     4501 ttgcaacgga tattacaaga gtgaattgaa atataaattt gcaacaaaca acagtgatag
     4561 tctaagctct catgatgagg cagtcttgct tacggaagag ctattaagaa gaggaatttc
     4621 caaggaagat tgcatactaa taatcggcgg taatttttta agaattttca ataatatatt
     4681 agcgtaggcg gtaaatgtaa atgacaggat tagataagtg gcaatatttt attaaagagt
     4741 gggtttttcc cgttgttatt gccttaatta tagttttatt tcttaataaa ttcgtattta
     4801 tattggtaac agttcctacc ggctctatgg aagatacaat attacccggt gacagattgt
     4861 atataaatga actgtttgat ataaatgatg caaaaagagg agatatatta gtattcaagt
     4921 ccgatgaatt agaggataaa agacttgtga aaaggcttat aggattgccg ggagaaaccg
     4981 ttgaaataaa aagagacgga agtatttatg tagacggaga aaaaattgaa gagccatatg
     5041 caataaactt caggggagaa gataaagtat ttatcgtacc ggaaaaatct tattttttct
     5101 taggcgataa cagaccaata tcttatgatg caagatattg gaatgagcct tatataagtg
     5161 aagataaagt aatcggcaag gttgaattca ggtttttnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnaat gtaatcatta aaaagcctgc ttcaagagga tatgaaaatg
     5341 cacctgtagt aatgcttcaa gcccacatgg acatggtgtg tgagaaaaat gaaggtgtcg
     5401 agcatgattt tgacaaagac ccgattaaat taagaataga agacggatat atttatgctg
     5461 ctgacacaac ccttggagcc gataacggaa taggagtggc ttttgcattg tctgtgcttg
     5521 attccgagct ggagcatcct tcccttgaag tattaataac aacagatgaa gaaaagggaa
     5581 tgacaggagc tgataaactt gacgggagct tgtttaaaag caagtattta ttgaatttag
     5641 acagcgaaga agaaggagtg tttactgccg gatgtgcagg aggaagtgaa attgatttta
     5701 aaatacctgt taaatatcaa aacacaaagg gcagggcata taaaataagt gttaaaggat
     5761 tgtccggagg tcactcggga gtagatattc ataaagaaaa agggaatgca aataaaattc
     5821 ttggaagaat tctttatgat ttaatagatg atgttgaatt agcaagcatt gagggtggcg
     5881 caaaaacaaa tgcaataccg agagaagcaa atgcaatagt aacgataaag tcagacaagg
     5941 tacctgaaaa aatagctaaa tggaacaaaa tactcaaaaa tgagtatgca tttaccgatc
     6001 cgtcaataag ggtagcatta acggatttaa atgaagaagt atttcctttg gagaaagaaa
     6061 tatttaaaaa aacagttgct ttaataaatt taattcccgt tggtgtacta tcaagaagca
     6121 cagctattga tttggttata agttccaata acttgggtgt cataagaact gatgaaaaat
     6181 atataacact ttttaatcac cctcgaagca gtgtggaatc attgcttacc gaagacttta
     6241 ttcctgccat gaaacagtta gcatctatat tggatgtaga gtatgtattg ggaagttatt
     6301 atccgggctg ggaatatgca aaggaatcta aaatcagaga tatttgcatt gagg
//
LOCUS       DAOPUE010000099         6341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12216_length_6341_cov_10.897073, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000099 DAOPUE010000000
VERSION     DAOPUE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6341
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12216_length_6341_cov_10.897073"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1309
                     /locus_tag="PKH42_08105"
     CDS             203..1309
                     /locus_tag="PKH42_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOA20435.1"
                     /translation="MKKVLTLLLITVFIFTGCTAGQETKVNEEEAYTAVEVETLMPMD
                     LYIENLMTGKVFADKDVFVIPLMAGKVERVNVKVGDKVEKDDVLFVMDKDDIEKQVNQ
                     AYAAYEAAKAGFEVSAAQIQSAKDNLERIKKLYEEGAVPETQYEQAKLAASDESLEAA
                     RMGVEQARVAYSNAASMLKNAEVKAPISGVISSVNIVEGEYATSSNPPVTIVDSDSVT
                     IEFGISASLVNKVKAGDKTLVEISSAGYKNEAVINSVSSSADIMTNLYTVSIVLENDG
                     AIKPGMFAKVYLKTDNIANALAVRAEAVKEREDKKYVFVAEDNSAIEKEVTTGLDTGA
                     YIEIKTGLSEGDKVIVKGQDYISHGSKIKVVRGE"
     gene            1312..4389
                     /locus_tag="PKH42_08110"
     CDS             1312..4389
                     /locus_tag="PKH42_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOA20436.1"
                     /translation="MLARFSVKKPVTITMMILIVIVIGVVSFSKLQIDLLPQMELPYV
                     MVQTTYQGAGPEEIENMISRPLEQTLSTVENIEDIISISNEGSSLLLMQFAFNTDLDE
                     IMLQIRERIDVIKSFLPDGTSTPLALKMDPNAIPIIQLAVSSKGDLYTTQKIAEDVIS
                     PRFERIEGTASASVSGGLEQEVEIMLKEETVKGYNLNSSYVAQILQAENLNMPGGTVK
                     KGNNELTVRTMGEFKSVEEIRNLIIPLMKGGTVRLKDIADVNLKNKEQSSITKLNGKE
                     VVQISVMKQSDANTVNVANDIEKEIERIKTEYPELDLVTVFNQADYINFAIDNLIGNT
                     TKGGILAILILLIFLRSFKTTLVIAISIPFSIITTFVILFFTDITLNMMSIGALALAV
                     GMLVDNSIVVLENIYRNRSLGMDRFTASVDGTNEVSMAVTASTLTTIAVFIPIVFTGG
                     LAAVLFKEFALTITIALLSSLVIALTLVPMLSSKLVSVKNLESEEEQEKKLGPLVVFY
                     KKILSYSLKHRLITILISIVLFVVSVIMVISVGAEFFPAGDEGMINVSVSLPAGSETQ
                     EIDAVLNEIQETITEIPEIEDVFTSAGGGNMYGMGGSGGSGSMIVLLRDFDERDKSAK
                     EISDEIRIMTKDIPGAEISVSESSTMMLGLTAGAISISIKGDDLDTLKAIGDDFKKII
                     EKVEGTREVTTSYEDGIPQVQIIADRGIASQYGLTTAQIGRAVINTLSGSNVTKYKIN
                     GDEIDVVLKGDNIYGESISYLEMLPVETPMGSNVPLSEVADIRIEKGPISILRENQVR
                     VLTVSGSVMGRDVQSASIEIEELLKEYEMPYGYSYILGGEIEQIQETFTDLFLVMLMA
                     IALVYMIIAAQFESLIQPLSIMFSVPLALSGGFIGLFITGLPLNVIGIIGLIILVGVV
                     VNNAIVLIDYINNRRRRGEDRNVAIMKAGPIRIRPIMMTALTTILGLVPMSLGIGEGA
                     EFTQSMGVVVIGGLSLSTVLTLVIVPVMYTIFDDISEFFKKKIKKDETTELIL"
     gene            4409..4999
                     /locus_tag="PKH42_08115"
     CDS             4409..4999
                     /locus_tag="PKH42_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOA20437.1"
                     /translation="MREKNQKRQDILNSAMTLFRDKGFHNTKMEDIAIGAGVGKGTLY
                     EYFESKQDVFDEACIEYVDVIIENIEYVSNIDDTFKNKLLILFNGKLKMESEFADMSI
                     DNVLSSKNIISEKTIKTIIGKITEMYSLICKIIDQGKEEGVVKKNIQSEVLACLIVGT
                     LGEYSRLKLQNKEKEIKEDDVIFDLLYNGFAVKHQQ"
     gene            5009..6244
                     /locus_tag="PKH42_08120"
     CDS             5009..6244
                     /locus_tag="PKH42_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOA20438.1"
                     /translation="MKRKISLLIVLMLILSTLINVSATEEAVEGEETAEAAEETLPVN
                     EPLMLSMEDAVRIATENSREMWKIDDGIAQVKEARKDAKDAKNLAEIIMSIPLSQIEA
                     TFGPDFDVTSNQVERILAKNDYYIKFADSQQAQLEKNREMLLAGIEIETKSLYYNVLV
                     AEQTIVINKLNLSKAEEQLRVANLKFNNGSATKAEVLNAEMAVQKAKTELDSAVDDYS
                     IAKLNLLNKLELPFDTEYILTDTNFTYVPTEEINLAEAIEKAKAERPEILKVENNLEL
                     QKIETHAYTAYYTSNLRQHKAAKEKLKDAELNVPQAYKDVELDVRKSYLNLIKAERAL
                     INMEKTFELAKEAARINQLLYDNGMATMLNVLEANTGMYQAEIGYYQLLAAYNINKLM
                     FDNSHLIGSISGAPEGSGF"
BASE COUNT         2294 a          894 c         1359 g         1794 t
ORIGIN      
        1 aatttacaag aatatataaa tattgcatga aaggaggtct ttttaaatgc taacttacta
       61 ttttacagtt agcggtgcct ggcgccgcta atgggcacca ctaatgattt ttggtctctt
      121 aattatatta attgacagta aattttgaag gtgatataat ttcattaaaa ctgaccggtc
      181 ggtcgaaatt tcggagggca atttgaaaaa ggtattgact ttattattaa tcactgtatt
      241 tatttttacg ggctgcacag caggccagga aacaaaagta aatgaagaag aggcttatac
      301 tgctgtagaa gtagaaacac taatgcctat ggatttatac atagaaaacc ttatgactgg
      361 aaaagtattt gcagataagg acgtttttgt aattccttta atggctggaa aagttgaacg
      421 tgtaaatgta aaagtgggcg acaaagtaga aaaagatgat gtcttatttg ttatggataa
      481 agatgatata gagaaacagg tgaatcaagc ttatgctgct tatgaagcag caaaagcagg
      541 atttgaagta agtgctgctc aaatacaaag tgctaaagat aatctcgaga gaataaaaaa
      601 gctttatgaa gagggagcag ttcccgaaac tcagtatgaa caagcaaagc ttgctgcatc
      661 agatgagtct ttagaagctg caagaatggg tgttgaacaa gcaagggtag cttattcaaa
      721 tgcagcatcg atgttgaaaa atgctgaggt caaagctccc atatccggtg taatttcgag
      781 tgtaaacata gttgaaggag aatatgcaac atcatccaat cctcccgtaa caatagttga
      841 ttccgacagt gttacaattg agtttggaat atccgcaagc cttgtaaata aggttaaagc
      901 cggtgataag actcttgttg aaataagttc agcaggttat aaaaatgaag ccgtaataaa
      961 cagcgtaagc tcttcagcag atattatgac aaatctgtat accgtaagca tagtgctgga
     1021 aaatgacggt gcaattaaac ccggtatgtt tgccaaggtt tatctaaaaa cagataatat
     1081 tgcaaatgct ttagccgtaa gagcagaagc cgttaaagaa cgtgaggata aaaaatatgt
     1141 gtttgttgca gaggataaca gcgcaattga aaaagaagtt acaacaggac ttgacacagg
     1201 agcatacata gaaattaaaa caggtctctc agagggagat aaagttattg tgaaaggtca
     1261 ggattacata agtcatggca gtaaaataaa ggttgtaagg ggtgaataag aatgcttgca
     1321 agattttcgg ttaaaaaacc cgtaacaatc accatgatga tattaatagt gattgttata
     1381 ggggttgtat cctttagcaa gcttcagata gacttgcttc ctcaaatgga actgccttat
     1441 gtaatggttc aaacaaccta tcaaggggca ggacctgaag aaatagaaaa tatgatatct
     1501 agacccttgg agcaaacttt atccacggtt gaaaatattg aggatattat ttccatatct
     1561 aatgaaggaa gttcattatt attaatgcag tttgcattta atactgattt ggatgaaata
     1621 atgcttcaaa taagggaaag aattgatgtt ataaagagtt ttttgccgga tggcacatct
     1681 acgcccttgg ctttaaaaat ggaccccaat gctattccaa ttattcaatt ggctgtttca
     1741 agcaaaggag atttatacac aactcaaaag attgcggagg atgttatatc tccacgtttt
     1801 gagcgtatag aaggcactgc ttctgcttcc gtatccggag gattagagca ggaagtagaa
     1861 ataatgctga aagaagaaac ggttaaagga tataatttga attcctcata tgttgcacaa
     1921 atacttcagg cagagaattt aaatatgccc ggagggacag tcaaaaaagg gaataatgag
     1981 ctaacagtaa ggacaatggg cgaatttaaa tccgtcgaag aaataagaaa tttgataata
     2041 cctcttatga aaggcggtac tgtcaggctg aaagatattg ctgatgttaa tttgaaaaac
     2101 aaggagcaat catcaataac aaaattgaac ggcaaggaag ttgtccagat atcggttatg
     2161 aagcaatctg acgccaatac agtcaatgtt gcaaatgaca tagagaaaga aattgaaaga
     2221 attaaaacag aatatccgga gctggattta gttactgttt ttaaccaagc tgattatatt
     2281 aattttgcaa tagataattt gataggtaat acgacaaagg gtggtatttt agctatactg
     2341 atacttttga ttttcttgag aagttttaaa acaacattgg ttatagctat ttcaatacct
     2401 ttttccataa taaccacatt tgttattctg ttttttaccg atattacact aaatatgatg
     2461 tcaataggag ccttagcctt agcggtagga atgctggttg acaattccat tgttgttctt
     2521 gaaaacatat acagaaacag aagcttaggg atggacagat ttaccgcatc agtagatgga
     2581 acaaacgaag tttcaatggc agttacagct tccaccttaa ctactattgc cgtttttatt
     2641 ccgattgtgt ttaccggcgg gcttgccgcg gttttattta aagaatttgc acttaccata
     2701 acaattgctt tattaagttc tttggttatt gctcttacat tggtaccgat gctgtcatca
     2761 aaactggttt cggtaaaaaa cctcgaatca gaagaagagc aggagaaaaa gctcggtcct
     2821 ttggttgttt tctataaaaa aatattaagt tacagtttaa aacatcgttt aataacaata
     2881 ttgataagta tcgtattatt tgttgtaagc gtaataatgg taatatccgt gggagccgaa
     2941 tttttccctg caggtgacga aggtatgatt aatgtcagcg ttagtcttcc tgccggctct
     3001 gaaacgcagg aaatagatgc tgtattgaat gaaatacaag aaacaattac agagattcct
     3061 gaaattgaag atgtatttac ctctgcagga ggtggaaata tgtatggaat gggcggctcc
     3121 ggcggcagcg gctctatgat tgtattatta agggattttg atgaaaggga caaaagtgca
     3181 aaagaaatta gcgatgaaat aagaattatg actaaggata tccccggagc tgaaataagt
     3241 gtttccgaat catcaacaat gatgctggga ttgacggcgg gagcaataag cataagtatt
     3301 aaaggagatg atttagatac tttaaaagct attggagacg actttaaaaa aataatagaa
     3361 aaagttgaag gaacaagaga agtgaccaca agttatgaag acgggattcc tcaagttcaa
     3421 attatagcag acagaggaat tgcatcccaa tacggtttaa ctaccgctca gataggcagg
     3481 gctgttatta atacattatc cggttccaat gttacaaagt acaaaattaa cggcgatgaa
     3541 atagatgtgg ttttaaaagg tgacaatatt tacggtgaaa gtatttcata tttggaaatg
     3601 cttcctgttg aaaccccaat gggcagcaat gtgcccttat ctgaagttgc cgatataaga
     3661 atagaaaaag gaccaataag catacttaga gaaaatcagg taagagtact aaccgtatcg
     3721 ggaagtgtta tgggaagaga tgttcaatct gcctccattg aaatagaaga acttcttaaa
     3781 gaatatgaaa tgccttacgg ctacagttat atacttggcg gagaaataga gcagatacag
     3841 gaaactttta ctgacttatt cttggtgatg ctgatggcaa ttgccttggt ttatatgatt
     3901 attgcagcac agtttgaatc attgattcag cctttgtcaa ttatgttctc ggtgcccctt
     3961 gcattgtccg gaggatttat aggcctgttt attacagggc ttcctcttaa tgtaataggg
     4021 ataatagggt tgataatatt ggtaggcgtt gttgttaata atgcaatagt attaattgat
     4081 tacatcaata acaggcggag aagaggagaa gacaggaatg ttgccataat gaaggcaggt
     4141 ccaataagaa tacgtccaat aatgatgact gcattgacaa ccatcttagg attagtacct
     4201 atgtcgcttg gtataggcga aggtgctgaa tttactcagt cgatgggtgt tgtggtgata
     4261 ggaggattgt ccttgtcaac agtcttaacc ttagtgatag ttcctgttat gtatactata
     4321 ttcgatgata tttcagaatt ttttaagaaa aagataaaaa aagatgaaac tactgagttg
     4381 atactgtaag tataggaggc aaataggaat gcgtgaaaaa aatcaaaagc ggcaggatat
     4441 attaaattcc gccatgacat tattcagaga taaagggttt cataatacta agatggaaga
     4501 tattgcaata ggtgcaggtg ttggaaaagg aaccttatac gagtattttg aaagcaaaca
     4561 agatgtattt gatgaagcat gcattgaata tgttgatgta attattgaaa atatagaata
     4621 tgtcagtaat atagatgata catttaaaaa taaattattg attttattca atggaaaact
     4681 taagatggaa tcagagtttg ctgatatgag catagacaat gtattatcca gcaaaaacat
     4741 tatttcagaa aaaacaatca agacaataat aggaaaaatt actgaaatgt atagtttaat
     4801 atgcaaaata atagaccaag gcaaggagga gggcgtcgtc aaaaagaata ttcaatctga
     4861 agttttggca tgtttgattg taggtacttt gggtgaatac tcgagattaa aattacaaaa
     4921 taaagaaaaa gaaatcaaag aagatgatgt aatttttgat ttattgtata acggatttgc
     4981 tgtaaaacat caacaatagg aggaaaggat gaaaagaaaa atatctttat taatagtact
     5041 gatgcttata ttaagtacct tgataaatgt gtcagcaaca gaagaagcag tggaaggaga
     5101 agaaacggca gaagctgcag aagagacact gccggttaat gagcctctaa tgctgtcaat
     5161 ggaagatgct gtcagaatag caacagaaaa cagcagagaa atgtggaaaa tcgatgatgg
     5221 aattgctcag gttaaagaag caagaaaaga tgctaaggat gctaaaaact tggcagagat
     5281 aataatgagc attccattat ctcaaattga agcaacattt ggtccggact ttgatgtaac
     5341 aagtaatcag gttgaaagaa tactggcaaa aaatgattat tatattaaat ttgccgactc
     5401 ccagcaggca caattggaga aaaacagaga aatgcttttg gcaggcattg aaatagaaac
     5461 gaaatcatta tactacaatg tactggttgc cgagcaaaca attgtaatta acaagttaaa
     5521 tttaagtaaa gcagaggaac aattaagagt tgcaaattta aaatttaata acggttctgc
     5581 tacaaaggcg gaagtattaa atgctgaaat ggcagttcag aaagctaaga ctgaattgga
     5641 ttctgcagta gacgattaca gcattgcaaa gttaaattta ttaaataaac tggaactacc
     5701 atttgatact gaatatatac ttacggatac caattttaca tatgtaccga ctgaagaaat
     5761 taatttagct gaagcaatag agaaagcaaa agcagaaaga cctgaaatac tgaaggttga
     5821 aaataattta gaacttcaaa aaatagaaac gcatgcttat actgcatact atacttcaaa
     5881 cttaagacag cacaaagccg ccaaagaaaa actcaaggat gcagagttaa atgtaccgca
     5941 agcatacaag gacgttgaac ttgatgtaag aaaatcgtat ttgaacttga ttaaggcaga
     6001 aagagcccta attaacatgg aaaaaacttt tgaattggca aaagaggcag cgaggataaa
     6061 tcagcttttg tatgataacg gaatggcaac tatgctgaat gtattggagg caaatacagg
     6121 catgtatcag gcagaaattg gatattatca gctattggct gcatacaata taaacaaatt
     6181 gatgttcgat aactctcatt taatcggcag tataagcggg gcaccggaag gatccggttt
     6241 ttaaggacgc tctttcgttt ttatgaaaat ttcagtcatt gttgggaaaa ggggttttgt
     6301 caaaaggttt acatatataa atttatgtaa atctaaaatt c
//
LOCUS       DAOPUE010000100         6281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12381_length_6281_cov_7.993415, whole genome shotgun sequence.
ACCESSION   DAOPUE010000100 DAOPUE010000000
VERSION     DAOPUE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_6281_cov_7.993415"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..774)
                     /locus_tag="PKH42_08125"
     CDS             complement(217..774)
                     /locus_tag="PKH42_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOA20439.1"
                     /translation="MIMEHSILIAGSGGQGIMLIGKLLSYAAIEAGKEATFYPAYGAE
                     MRGGTANCTVVISDEEISSPILERFTTIIALNEPSYIKFHSQVIDGGNILINSSLIAS
                     ISEELGKKASVYAVPVNNIALELGSEKISNIVLIGAYIGITGIIDPEVFRNTIRKMLY
                     KDEELMNLNLIAFDKGLAAGQDFRL"
     gene            complement(771..1517)
                     /locus_tag="PKH42_08130"
     CDS             complement(771..1517)
                     /locus_tag="PKH42_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOA20440.1"
                     /translation="MNKVYEKPKLIPADLSNSWCPGCGHGTIHKLIANTIDTYRMKEE
                     TIMISPVGCGTMNTATGWFDCNSVACAHGRASAAAYAIKKCYPDRLVFAYQGDGDLAS
                     IGMAETIHTANRGGNLTIIFVNNGNFGMTGGQMAPTSLIGQRTTTSQEGRNFKDHGNP
                     IGVCELIATLEMPKYVVRVSLHDAKHVIQTQKAIEKAFRMQSEGKGFSFVEVLSPCPT
                     NWKMSPVESMKHINDVVIKTYPLGVFKEDK"
     gene            complement(1510..2592)
                     /gene="vorB"
                     /locus_tag="PKH42_08135"
     CDS             complement(1510..2592)
                     /gene="vorB"
                     /locus_tag="PKH42_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOA20441.1"
                     /translation="MEKIFLKGNDAMAEAAIRAGCRFFAGYPITPQNEIPEYLSKRLP
                     EVNGVFIQGESEIASISMVYGVASTGIRSMTSSSGPGLCLKTEGINFLVAAEIPAVII
                     DVMRAGPAIGGIQPAQQDYTFITGGAGPGGVRCFVLAPYTVQEAVDLVYEAFDIADKF
                     RNPVIVMVDGAIGNMMENIILPELRELDTLPQHEDWKITKRDADGKARIIQTYIHSVI
                     GLEEANKRMAEKYKIWEDYEQRYEAIMLEDAEIVVTAYGIVARMAKAAVKELRKEGIK
                     VGMIRPIAIYPFPKKAFDSLDYGRVKQIWDIELSIPSQMFRDVDYTVRGRAPVYQLLR
                     SGGMMTSKESIIKAIREKALEGENYE"
     gene            complement(2603..2806)
                     /locus_tag="PKH42_08140"
     CDS             complement(2603..2806)
                     /locus_tag="PKH42_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOA20442.1"
                     /translation="MAKIIISENLCKGCSLCVTQCPKNIIKISNKINSKGYTIAEQTD
                     DTQCTGCTLCAIVCPDSAIEVYK"
     gene            complement(2849..4360)
                     /locus_tag="PKH42_08145"
     CDS             complement(2849..4360)
                     /locus_tag="PKH42_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOA20443.1"
                     /translation="MEKQSELSKAKQIDNNERGQWSSKIGFLMASIGSSVGLGNIWGF
                     PYKMGANGGFAFLIIYLALLVFVGFAALLGEFTLGRMTGEGIIGTYRTLNKKFTFVGW
                     FGVISAFLVISFYCVLGGFVLRYAVGFLMEIFSAGSGFGGLGSGEFFGSFIVNGFSSA
                     FYFFIFLGLTSVIVMGGVKGGLEKFTTFAMPTLSIIMIIVIIKSLTLEGAIEGVKFMF
                     EPNWQAFSEIGFVKILKTAAGQMFFSLSLGMGIMVIYGSYLSKQEDIQKSSIVIGVAD
                     TIFAVLAGLMIMPAVGAFMAQGVEGITYGAGPGLLFITMHQVFSVGMGGFMGNLFGFM
                     FYMLVFFAAITSSISVLEVCIAFFVDKRLKEGKSSQRIKITAIVCIVIFILGLPVTLD
                     GLGSGTAGGAAIDIPAVMFGMSEVRVAFDCWLDFYDMISEGLLMPLGTMLMCLFIGWI
                     IGTKAIVKELEETPKVKIISYRFWEICFKYIAPVIVFIVFLGQIDDFFGLGIF"
     gene            4681..5379
                     /locus_tag="PKH42_08150"
     CDS             4681..5379
                     /locus_tag="PKH42_08150"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOA20444.1"
                     /translation="MNKKTLELYIESNPFEQLNKIVFYMLLKEISSLKYKPGQKLNVS
                     QISSDLQISRTPITEALKMLVEYGLVKDNLDKQGYYVSVLSRKEAIDIHKARKALESM
                     AAFICAENGSCPNIDKMEELAYRFVDFFKKEKYEIVDAVDVPFHKLIIESSGNKYIQR
                     CYKLLERQLYRYQCYSMSNVMNDNINPISNELEYQHISIVNAIKLHLPNLARQSMEQH
                     IDSCMMLLNGYMRN"
     gene            complement(5486..6118)
                     /locus_tag="PKH42_08155"
     CDS             complement(5486..6118)
                     /locus_tag="PKH42_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOA20445.1"
                     /translation="MIDTVIFDFDGTLADTNNLVLNSFKHIYNKYYDGKWDEEYVLST
                     FGEPLALTLKRDFSGFAYEEVLASYREYQADRFEKEVTLYDTVSETVKYLHKKDIKIG
                     IATSRIKPPTLFALKNFDLDKYFQVVISAEDVEKHKPDKEPLIKAIQGLDSSAENTLY
                     VGDSKFDMECALNAEVTPVLVGWQKNSGELAEKYKIKHVLSKMWDLTWLI"
     gene            complement(6111..>6281)
                     /locus_tag="PKH42_08160"
     CDS             complement(6111..>6281)
                     /locus_tag="PKH42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOA20446.1"
                     /translation="VKQDGLIHLSEMSDKYIKNPMEVVQVGDTVNVTIISIDKERQRI
                     GLSMKKSRSKND"
BASE COUNT         1984 a         1318 c          945 g         2034 t
ORIGIN      
        1 tatagtgcat attctttcat ttttttcgac aatcaaattt ttaaattcca taactcctcc
       61 tataaattct tttcagcttt attatagcat ttaaaggaag atttttcatt aatttattca
      121 tataaaatta aagcaaaaaa caagtaccca atatgacaga tgtatatgtt ctaccgggta
      181 cttcaataag tatatctgaa cagctgattt taacagttat aaacgaaagt cctgacctgc
      241 cgccaaccct ttatcaaaag caattaaatt caggttcata agttcttcat ctttatataa
      301 catttttctt atagtatttc tgaaaacctc cggatctatt attccggtaa ttcctatata
      361 ggcaccaatc aaaactatgt ttgagatttt ctcgctgcct agctcaagtg caatattatt
      421 gacagggaca gcataaacag atgctttttt ccccagttct tcagaaatcg aagctatcaa
      481 tgacgaatta ataagtatat ttcctccatc aattacctgt gagtggaatt ttatataact
      541 tggttcatta agggcaataa tcgtagtgaa acgctctaaa ataggagagg atatctcttc
      601 gtctgaaatt acaactgtac aattcgcagt accacctctc atttcagcgc cataagcagg
      661 ataaaatgtc gcttccttac cagcttcaat tgctgcatag cttagcaact tgcctattaa
      721 catgattccc tggccgcctg atccggcgat tagtatagag tgttccataa tcatttatcc
      781 tccttaaata cacctaaagg gtaagtctta ataacgacat cattaatatg tttcattgat
      841 tccaccggac tcattttcca atttgtagga caaggtgaaa gaacctcgac aaatgaaaat
      901 cccttgcctt cactctgcat tcgaaaagct ttttctattg ccttttgtgt ttgtattaca
      961 tgctttgcat catgcagaga aacccgcaca acatatttag gcatttccaa agtagcaatt
     1021 aattcacata caccaatcgg gttgccatgg tctttaaaat ttcttccttc ttgcgatgta
     1081 gtcgtcctct ggccaattaa gctggtaggt gccatttgac cgccggtcat tccaaaattt
     1141 ccgttattca caaaaattat cgttagatta cctccacggt tagcagtgtg aattgtttct
     1201 gccatgccta tggaagcaag atccccatct ccctgatacg caaaaaccag tctgtcaggg
     1261 taacactttt ttatggcgta agcagcagca gaagcacggc catgtgcgca agcaactgag
     1321 ttgcaatcaa accaaccagt tgcagtattc attgtgccgc atcccacagg acttatcatg
     1381 attgtctctt ctttcatgcg ataagtatcg atagtatttg caatcaattt gtgtatagtt
     1441 ccgtgaccgc aaccggggca ccaggaatta cttaaatctg caggtattaa tttgggtttt
     1501 tcatatactt tattcataat tctctccttc cagtgccttt tcccttatag ccttaattat
     1561 gctttcttta cttgtcatca ttccacctga tcttaacagt tgataaactg gtgctcttcc
     1621 gcgaactgta taatctacat cccggaacat ttgcgatggt atggaaagtt ctatatccca
     1681 aatttgttta accctgccat aatccaatga atcaaacgct ttcttaggga atggatatat
     1741 tgcaatcgga cgtatcatcc ctactttgat gccttctttt cttaattctt taacagcagc
     1801 ttttgccatg cgggctacta ttccatatgc tgtaacaaca atttcagcat cctccaacat
     1861 tattgcttca tacctttgtt cataatcctc ccaaattttg tatttctctg ccatgcgttt
     1921 atttgcttct tccaatccga ttactgaatg aatgtacgtt tgtattattc tagctttacc
     1981 atctgcatcc cttttagtaa ttttccaatc ctcatgttgc ggcaaagtat caagctccct
     2041 caattcggga agaattatat tttccatcat atttccaatc gcgccatcca ccattacaat
     2101 aaccggattg cggaatttat cagctatgtc aaaggcttca tagacaaggt ctactgcttc
     2161 ttgcacggta tatggggcaa gaacaaaaca tcgtacacct ccgggtcctg cccctccggt
     2221 aataaatgtg taatcttgtt gtgctggttg aattcctcca attgccgggc cggccctcat
     2281 aacatcaatt atcacggctg gtatttcagc tgccactaaa aaattaatac cttcagtttt
     2341 taaacaaagt cctggtcctg atgaagatgt catgcttctg ataccggtag atgcaacacc
     2401 atataccata gaaattgatg caatttcgct ttctccctga ataaatactc cattaacttc
     2461 cggaagtctt ttagaaagat attcaggaat ttcattttgt ggtgtaatag gatatccggc
     2521 aaaaaaacgg catccagctc ttattgctgc ttctgccatt gcatcgtttc cctttagaaa
     2581 aattttttcc atcttcatcc tcctatttat aaacttcaat agctgagtcg ggacacacta
     2641 tagcacatag tgtacaacct gtgcattgtg tatcgtctgt ctgttctgct attgtataac
     2701 cctttgaatt gattttattg ctgattttta tgatattctt cggacattgg gtcacacaga
     2761 gactgcatcc cttacataaa ttttctgaaa ttataatttt agccatacag gttctcctta
     2821 ttaagagttt tataaaaaaa ccagtttttt agaatattcc taatccaaag aaatcatcta
     2881 tctgccccaa aaatacgatg aaaacaatta ccggtgcaat atacttaaag caaatttccc
     2941 agaaacgata actaattatt tttactttag gagtctcttc taactctttt actattgcct
     3001 ttgttcctat aatccacccg ataaacaaac acatcagcat tgtgcctaac ggcataagca
     3061 aaccttcact aatcatatca taaaaatcca gccagcagtc gaaagcaacc ctgacctcac
     3121 tcataccaaa catgacagca ggaatatcaa tagctgcgcc tccggctgta cctgagccaa
     3181 gaccatccag cgtaaccggt aaccccagaa tgaatataac tatacaaacg atagcagtta
     3241 tcttgattct ttggctgctt tttccttctt ttaaacgttt atctacaaaa aaagctatac
     3301 atacctcaag cacagaaata gatgaggtaa tggctgcaaa aaatacaagc atgtaaaaca
     3361 taaatccaaa aagatttccc atgaaacctc ccatccccac actaaatact tgatgcattg
     3421 taataaacag taacccagga cctgcaccgt atgtgatacc ctcaacacct tgtgccataa
     3481 aagcccctac tgcaggcatt atcattagtc ctgcaagcac tgcaaatata gtgtctgcca
     3541 ctccaataac aatagaactt ttctgaatat cttcttgttt gcttaaatat gacccatata
     3601 taaccattat acccattcca agagacagtg agaaaaacat ttgtccggct gctgttttca
     3661 gaattttaac aaaacctatt tccgaaaacg cttgccaatt tggttcgaac ataaatttta
     3721 ctccttcaat tgcaccctca agagttaggc ttttaattat tacaatgatc ataataatag
     3781 aaagggttgg cattgcaaat gtcgtgaact tttctaatcc tccttttact ccgcccatta
     3841 caataactga tgttaatccc agaaatatga agaaataaaa agcgctggaa aagccattca
     3901 caataaagga accaaaaaat tcaccgctgc ctaagccacc aaatccagaa ccggcactaa
     3961 atatttccat caaaaatcca actgcatatc gtaatacaaa tccacccagg acacaataga
     4021 aggatataac taaaaacgct gaaatcactc cgaaccagcc gacaaaagtg aattttttgt
     4081 ttaaagttct gtatgtgccg ataatacctt cacccgtcat tcttcccagt gtaaattcac
     4141 ctaataacgc tgcaaaacca acaaaaacta atagcgctaa atagatgatc agaaaagcaa
     4201 acccgccgtt tgcacccatc ttataaggaa atccccaaat gtttcccaaa ccaacagatg
     4261 aaccgatgga tgccattaga aaacctattt ttgaactcca ttgacctctt tcattgttgt
     4321 caatttgttt tgcttttgat aactcagatt gtttttccat aaataaatcg tcctcctatt
     4381 agattatatt agtacataaa tacataaata catattaaca actaacattc actgtgtcaa
     4441 aattcaaacg tttttctgca taaatactta aataatacta tattaagcac aatatattag
     4501 aaaaaatagt ttttctgcat ttattaaaaa agttttctgt gtacaactta ttttaattgt
     4561 gttataataa aaaaactaca taaaaattgt cataaccggc acttgataat ttttaaactg
     4621 ttatatatat aaaacagttc aattttacca ttcaatctaa gattagacag gagtaatatt
     4681 atgaataaaa aaacattaga actatatatt gagtcaaatc cttttgaaca gcttaataag
     4741 attgttttct acatgttatt aaaagaaatt tcttccctga aatataagcc ggggcaaaaa
     4801 ttgaatgttt cacaaatctc ttctgatttg cagataagtc gcactcctat aaccgaggct
     4861 ttgaaaatgt tggtggaata cggattagtt aaagataatc tggataaaca agggtattat
     4921 gtttctgttt taagcaggaa agaggcaatc gacatacata aagcacgaaa agcacttgaa
     4981 agcatggctg catttatatg tgcagaaaac ggcagctgtc cgaatattga taaaatggaa
     5041 gaattagctt ataggtttgt tgattttttt aaaaaggaaa aatatgagat agtagatgca
     5101 gtagatgtcc cgtttcacaa gttaattatt gaatcaagcg gaaataagta tatacagcga
     5161 tgctataaat tattggagcg tcagctttat cgataccagt gttattctat gtctaacgta
     5221 atgaacgaca atattaatcc aatctctaat gaattagaat atcagcatat ttcaatagtt
     5281 aatgctatta agcttcatct ccctaattta gccaggcaat ctatggagca gcatattgat
     5341 tcctgcatga tgcttttaaa tggatacatg cgaaattaat acaaagataa ctgaagatac
     5401 ttttaattta aaaaacaaaa aaatgctcca atcatgttga tattacataa ttggagatat
     5461 atattaagta aatagtttat aaaagctata ttaaccaggt taaatcccac attttactga
     5521 gcacgtgctt aatcttgtat ttttccgcca attcaccgga gtttttctgc caccccacaa
     5581 ggacaggagt aacttctgca ttcagggcac attccatgtc aaatttagag tcgcctacat
     5641 acaaggtatt ttccgcactg ctgtctaatc cctggatggc ctttatcaag ggctctttat
     5701 caggcttgtg tttttctaca tcttcagcag aaataacaac ttgaaaatat ttgtcaagat
     5761 cgaaattttt taaggcaaat aaggtgggag gtttaatcct tgatgtggct attccgattt
     5821 ttatatcctt tttgtgcaga tactttacag tctcactgac agtgtcatat agggttactt
     5881 ctttttcaaa cctgtcagcc tgatactctc tgtaggatgc taaaacttct tcataggcga
     5941 agccgctgaa atcccgtttt aaagttaatg caagaggttc tccgaatgtg cttaaaacat
     6001 attcttcatc ccatttacca tcataatatt tgttgtaaat atgcttaaaa gagtttagta
     6061 ccaaattatt tgtatctgcc agtgttccgt caaaatcaaa tattaccgtg tcaatcattt
     6121 ttactcctgc tttttttcat gcttagacct attctttgtc tttctttatc tatgcttata
     6181 attgttacat tgacagtatc tcctacctga accacttcca tgggattttt gatatatttg
     6241 tcgctcattt cagaaagatg gatcaaaccg tcctgtttaa c
//
LOCUS       DAOPUE010000101         6214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12536_length_6214_cov_7.429453, whole genome shotgun sequence.
ACCESSION   DAOPUE010000101 DAOPUE010000000
VERSION     DAOPUE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6214
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12536_length_6214_cov_7.429453"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKH42_08165"
     CDS             complement(<1..581)
                     /locus_tag="PKH42_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20447.1"
                     /translation="MRPLKRILCIIIAILMMVTMIPGNYLFAAPGDTEDDPIPYNRND
                     TTNFPQYPNEGYVRVDKKAVWLEDEDNIAKVTMTLDGAGVKQGTDAVLVIDRSGSMDD
                     NVNVTRTYEKEVEVEKQVPYNEVQLTYNASNVSHQYEERYLLILYRWQTATSNVSITL
                     TAYVDDDGNYKGYKNVSVSGFHSTDRNYRLNSS"
     gene            complement(596..2638)
                     /locus_tag="PKH42_08170"
     CDS             complement(596..2638)
                     /locus_tag="PKH42_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20448.1"
                     /translation="MKKKFNNKGSTMIMLVMAISIISVLGAAILGITLMNYKIKKTNT
                     DLKYSFYMSESGLDRAYAAAYAIILNSIEEANESAQDKIAEFNHDKLMDILLNGSEDD
                     KDKYLEDDDGDGVYDFKQSVIDEEAKIAFENKYKEKIEDDIETAILGEGYENDDEKLI
                     ISLSAPNYGEFSADNPLRVPVSSEYENKINDIARKTDVDIVIEVPDYNKSYTVSTVTM
                     DVNPYWTKAITAKNLNIYNDDTSFNGEVFVNNNVNIGTGAIAEFKKDLSVKRYINIDK
                     NGVLDTRNVYANGIFLNGEGAEFTAEKEDDESVGYSGIMVKDDLELNAKNQVVRINGP
                     YYGFATSQAREDTDNSAIIINDKDVQLEITGDLFLYGTSYIKDIPAKYATGESLSVKG
                     NYIAYTEGLDSDDVSILDEEDRNKFLESNIIFDYDSYTPLPRLAERFKDDNSEIQVYD
                     KAKYIYAYSKNNSSSLFLGTGSIRLLGIEKALHGSAINNGSIIRSNRGIDYMAQKSGE
                     IEAVYDKKIDKLGFDTEDVSQFTLDEIFDNIEKLSYAELAELNNKFNQATGQLVHINN
                     NPGGEYHFPSEAKSGLIITNENIIISGEVDFTGVIICDGDITIENDPTNPGGKKFKYN
                     KGVITTLIAEHNLYELIFKDTPSTGQKTSITTFKSGDDGVNVNFSELLRFENWKIK"
     gene            complement(2652..3341)
                     /locus_tag="PKH42_08175"
     CDS             complement(2652..3341)
                     /locus_tag="PKH42_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA20449.1"
                     /translation="MMRIRNNKGLTLVEILITLAVLGIVIMPLMSMFFTSQKMNNESE
                     MKYRAIQFAQEELESIKAMKILNTEDDGYPPRGDGSYGYVTSPDSEGYTIDVRIEKGA
                     EYEGSDIEPAGIPVFDRPIYIITDEDELEIDIAPGIEKIGIEIAIPYDNAKAKIRLVN
                     SDENLKVYIFKNKEHINSYKIMGDATVIEVAENSAKPDNQLYDITVTVEKDGNLIDII
                     RGTTVFEINPD"
     gene            complement(3358..4545)
                     /locus_tag="PKH42_08180"
     CDS             complement(3358..4545)
                     /locus_tag="PKH42_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20450.1"
                     /translation="MKLSKREKFLILLLIVTVIGYLVFTYVPFGKVFNLDELKAEHSQ
                     KKQAYDTMSQNIVQKNTFEEKVLSLTEEINSLNVISDLQQEKIIVFLHNYLNKNNINA
                     SNISFTDVETVEMSHLVIPDEAKESGTLQELMYEIDGRFDALNVEYPDADKKDGNQLL
                     ARKVSTNITFDGTYADMIDFIDSIQINPVDISITSVNTIAEGDILQGTMNLNFYEIPK
                     PIDFKETNNEWIWMDLAESGKENPFSTDATQMVFASGGNYDFYMSLQPEVSDLPSILI
                     GETNDVDRKTYISKDINSVESVNFAFKTENDKYYYRYSIASSVSYPKNDEWKEFIPNS
                     EGNINIVIYSKPRILKEDSSGANISVYNSTDLKVRFDVIDDDKTSPRAYFKDARTISV
                     IRK"
     gene            complement(4532..6190)
                     /gene="pilM"
                     /locus_tag="PKH42_08185"
     CDS             complement(4532..6190)
                     /gene="pilM"
                     /locus_tag="PKH42_08185"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOA20451.1"
                     /translation="MTKQGTKKSIRKVKDMGKVLSLEFSNKYIKMLDGSRSGNSLTVN
                     KSILLEVEKGCIDDGKIIDIDSVINTVNEAISKNSIKSRNAIFTIHTNSTVTRNIELP
                     VLKSKSDTMSMIKSELSQLMPVDLNQYKLIYKKTESVNADGVEKGKYIVYGLPTYMFE
                     QYMLLAEKLKLELIAFELAFNTLDKIYDKKLFINNNPLKSGVVSAFLDIGYDSLTFSV
                     VNNGKNDFSRTSQNGIKDIVKNFGAVHYLSDEEALKEIEKISLMPDDISEISKLNIIE
                     ENINIWIDELSRYIRYYNSVNKEKQIEKIYLYGIYSNIKGLPEYIESHINIETVLITS
                     LSNTVFRDKDNESITTYFTTALSLHMDKKDVNFLTDAKKQRKSTFSRRVALLGVSTAA
                     VLAFALAAYTYYIEKTALEKDIAVYDAFINNEDNIKLNSEAIALKKKTELMEKYMGEV
                     TKLQTAIKNEDAVNIIIFEQLSAAIPLGTKINSMSADIDTIKLQCSSATRQETAQFEK
                     NLKEIEFIDNVYIPAIVDGTEGSSSNYSYTVVCDLKDVISYEAE"
BASE COUNT         1728 a         1252 c          910 g         2324 t
ORIGIN      
        1 ctgcttgaat ttaatctgta atttctgtct gtagaatgaa acccgcttac ggaaacattt
       61 ttatatccct tatagttgcc gtcatcatcc acatatgcag tcagcgttat agaaacatta
      121 gaagtagcag tctgccatct atacaaaatc aataggtatc tctcttcata ctgatgactg
      181 acatttgaag cattgtaagt caactgtact tcattatagg gaacttgttt ttctacttca
      241 acctcttttt cataagtacg tgttacatta acattatcat ccatactgcc ggaacggtct
      301 ataacaagta ccgcatctgt tccttgcttt acacctgcac cgtccaatgt catggtaact
      361 ttggcaatgt tatcctcatc ctctaaccat actgcttttt tatcgacacg tacgtacccc
      421 tcatttggat attgcggaaa attggtagta tcattgcggt tataaggaat ggggtcatcc
      481 tcagtatctc ccggtgctgc aaataaatag tttcccggta tcatcgtaac catcatcaaa
      541 attgcaatta taatgcataa aattcttttt aaaggtctca ttttttaccc cctctttatt
      601 ttattttcca gttttcaaat ctcaataatt cgctgaaatt aacatttacg ccgtcatctc
      661 ctgatttaaa tgtagtaatg cttgtttttt gtcccgtgct aggagtatcc ttaaatatta
      721 actcgtaaag attatgctcc gcaatgagag tcgtaataac tcccttattg tatttaaatt
      781 ttttgccgcc gggatttgtg ggatcatttt caattgtaat atcaccgtca catattatta
      841 caccggtaaa atccacttct cctgatatta taatattttc atttgttata atcaatccgc
      901 tttttgcttc cgaaggaaaa tggtattctc ctcccggatt attatttata tgcaccaact
      961 gacctgtagc ctgattaaat ttgttattta attcagctaa ttcagcgtaa gataattttt
     1021 ctatattatc aaatatctca tctaatgtaa attggctcac atcttcagtg tcaaatccga
     1081 gtttatcgat ttttttatcg tataccgctt caatttcacc gcttttttgt gccatataat
     1141 caattcctct atttgaacga attatgcttc cgttatttat ggctgagccg tgcaatgctt
     1201 tttctatacc taaaagtctt attgagcccg ttcccaaaaa taaagatgaa gagttgtttt
     1261 tactgtatgc atatatgtat tttgctttat catatacctg tatttcacta ttatcatcct
     1321 taaatctttc tgcgagtctg ggcaagggag tataactgtc ataatcgaat ataatattgg
     1381 attcaagaaa tttgtttcta tcctcttcat ccaaaatact tacatcatca gaatccaaac
     1441 cctcggtata ggcaatataa tttcctttta ccgacaggga ttcccccgtg gcatattttg
     1501 caggtatatc ctttatatat gaagtgccgt ataagaataa gtcgcctgtt atttctaatt
     1561 gaacatcctt atcattaata attattgcac tgttgtcggt atcctctctt gcctggctgg
     1621 tagcgaagcc atagtatgga ccgttaattc tgacaacctg gttttttgca tttaattcca
     1681 aatcgtcttt taccataatc ccggaataac cgacactttc gtcgtcttct ttctctgccg
     1741 taaactcagc accttcgcca tttaaaaaga ttccgtttgc atatacattt cttgtatcta
     1801 agacaccgtt tttatctatg ttaatatacc tttttacgga caaatccttt ttaaattcag
     1861 ctattgcacc agtacctata ttaacgttat tattaacgaa tacctccccg ttaaaactgg
     1921 tgtcatcatt gtaaatgttt aggttctttg cggtgattgc ctttgtccag tatgggttaa
     1981 catccattgt tacggtagac acagtatagg atttgttata atcaggcacc tcaattacaa
     2041 tatcaacatc tgttttcctg gctatatcat ttattttatt ttcatattca gagcttacag
     2101 gtactcgcaa aggattgtct gcgctgaatt cgccataatt tggcgctgat aaagatatta
     2161 tcagtttttc atcatcattt tcgtatcctt cacctagtat tgcagtctct atatcatctt
     2221 caatcttttc cttatactta ttttcaaagg ctatttttgc ttcctcatca ataactgatt
     2281 gcttaaaatc ataaacaccg tctccgtcat cgtcctcaag atatttgtct ttatcatctt
     2341 cagagccgtt taataatatg tccataagct tgtcatgatt aaattctgca attttatcct
     2401 gagcactttc atttgcttct tcaatggaat ttagaattat tgcataggct gcggcatatg
     2461 ctctgtcaag tcccgattca gacatataaa atgaatattt caaatccgta ttggttttct
     2521 ttatcttata attcattaga gttatgccta atattgctgc acctaaaacg gaaattatag
     2581 atattgccat aaccagcata atcatggtgg agcctttatt gttaaatttc tttttcatta
     2641 tatcaccttc tctaatcagg attgatttca aatacggttg ttccccttat tatatcgatt
     2701 aaattgccgt ctttttcaac agtcaccgtt atatcataca gttgattgtc cggttttgcg
     2761 gaattttcag caacttcaat aacagttgca tcacccatta tcttataact atttatatgc
     2821 tccttatttt tgaaaatata tacttttaaa ttttcgtccg aatttaccaa ccttattttc
     2881 gcttttgcat tatcgtaagg aatggcaatt tcgataccga ttttctcgat tcccggagca
     2941 atatcaattt caagttcatc ttcatcggtg attatgtata ttggtctgtc aaacacgggt
     3001 ataccggcag gctcaatatc gctgccttca tattcagccc ctttttcaat cctaacatct
     3061 atggtgtaac cttccgaatc aggtgaggtc acataaccgt agcttccgtc ccctctggga
     3121 ggatatccgt cgtcttcggt attcagaatc ttcattgcct taatagattc taactcttcc
     3181 tgggcaaact gaattgctct gtacttcatt tcgctttcat tgttcatttt ttgagaggta
     3241 aaaaacatag acataagggg cataataact atacccaaca ctgcaagggt tatcaatatt
     3301 tccaccaatg ttaatccttt attgtttctt atcctcatca tcgtactgcc tctccatcta
     3361 ctttcttatt acacttattg ttcttgcatc tttgaaatat gccctcgggc ttgttttgtc
     3421 atcatcgata acatcaaatc taaccttcaa atccgttgaa ttatatacgg atatattggc
     3481 tcctgaggag tcttccttca aaatcctcgg ttttgaatat attacaatat ttatatttcc
     3541 ttccgaatta ggaataaatt ctttccactc atcatttttc ggatatgata cactgcttgc
     3601 tatggagtat ctgtaataat acttgtcgtt ttcggttttg aaagcaaagt ttacactttc
     3661 cactgaattt atatccttag atatataagt tttcctgtcc acatcatttg tttcacctat
     3721 caaaatcgag ggtaaatcgg aaacttcagg ctgcaggctc atataaaaat cataattgcc
     3781 ccctgatgca aaaaccattt gagttgcatc cgttgaaaaa ggattttctt tacccgactc
     3841 agccaaatcc atccatatcc actcattgtt agtttctttg aagtctatcg gtttaggaat
     3901 ttcgtagaag ttcaaattca ttgttccctg aagtatatcg ccttccgcaa tggtatttac
     3961 acttgtaatt gatatatcta caggattgat ttgtattgag tcgataaaat caatcatatc
     4021 ggcatatgtc ccatcgaatg ttatattagt ggatactttc cgtgccaaca gttgatttcc
     4081 atccttttta tctgcatcag gatactcaac gtttagagca tcgaatcttc cgtcaatttc
     4141 atacattaat tcctgaagag tgcctgattc ttttgcctca tcgggaatta caagatgact
     4201 catctcaacc gtttctacat ccgtaaagct tatgttgctt gcattaatat tgtttttatt
     4261 caaataatta tgtaaaaata caattatttt ttcctgctgc aagtctgaaa taacatttaa
     4321 gctgttaatc tcctcagtta aagacagaac tttttcttca aaagtatttt tttgcacaat
     4381 attctgcgac atagtatcat atgcctgctt tttttgtgaa tgctctgcct taagttcatc
     4441 caggttaaaa acttttccaa acggtacgta tgtaaatact aaatacccaa ttactgtaac
     4501 aattaacagc aatattaaaa atttctctct cttactcagc ttcataagat atcacatcct
     4561 ttaagtcaca aacaacggta taggagtaat tggaactgct tccttctgtt ccgtcaacta
     4621 tagccggtat gtagacatta tctataaatt caatttcctt aaggtttttc tcaaattgag
     4681 cggtttcctg tctggtggca gaagagcact gcagctttat ggtatcaata tcggcactca
     4741 ttgaatttat tttagtacca agaggaatgg ctgccgaaag ctgttcaaat attattatat
     4801 ttacggcatc ctcattcttt atcgccgttt gaagttttgt tacttcaccc atatatttct
     4861 ccatcagctc agttttcttt tttaaagcaa ttgcttcgct gttaagcttg atattgtctt
     4921 cattgtttat aaaagcatca taaaccgcta tgtctttttc taaggctgtc ttctctatat
     4981 aataagtata tgcggcaaga gcaaaggcta aaactgccgc cgttgaaact ccgagaaggg
     5041 caactcttct gctgaatgtg ctttttcttt gttttttcgc atctgttaaa aagtttacat
     5101 ccttcttatc catatgtaag gatagggcag tagtgaaata tgttgtaata ctttcattat
     5161 ccttatctct gaaaacagtg tttgacaatg atgtaataag cactgtttca atatttatat
     5221 gcgattcgat atattccggc aatcccttta tatttgaata tatgccgtac aaataaatct
     5281 tttctatctg tttttcctta tttactgaat tgtaatacct gatatacctt gaaagctcat
     5341 caatccaaat atttatattt tcctcgataa tattaagttt tgaaatttcg gaaatatcat
     5401 caggcattaa tgatattttt tctatttctt ttaatgcctc ctcatcgctt aaataatgaa
     5461 ccgcaccaaa atttttaaca atgtccttta tgccgttttg ggaggttctg gaaaaatcgt
     5521 tttttccgtt atttacaaca ctgaatgtca gactgtcata acctatatcc aagaatgcag
     5581 aaacgacacc ggattttaaa ggattgttat ttatgaaaag ttttttgtca taaattttat
     5641 ccaaagtgtt aaatgcaagt tcaaatgcaa taagttcaag cttaagcttt tcggccaaga
     5701 gcatatactg ctcaaacatg tatgtaggga ggccgtaaac aatatatttg cctttttcca
     5761 caccgtctgc attaacagac tcagtctttt tgtaaatcag cttgtattga ttcaaatcaa
     5821 caggcataag ctggcttaat tcgcttttaa tcatggacat agtgtctgac ttgctcttta
     5881 aaacaggtag ttctatgttt cttgtaacgg ttgaatttgt atgaatggta aaaattgcat
     5941 ttcttgattt tatgctgttt ttgcttatgg cttcattgac cgtattaatt actgaatcaa
     6001 tatcaataat tttaccgtca tcaatacatc ctttttccac ttccaacaaa atacttttat
     6061 taacagttag tgaattcccg ctccttgagc cgtccaacat tttaatatat ttgttgctga
     6121 attctaaaga taaaactttc cccatgtctt ttacctttct tatagatttt tttgttcctt
     6181 gctttgtcat cctgagcgta agcgaaggat ctca
//
LOCUS       DAOPUE010000102         6152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12701_length_6152_cov_8.958504, whole genome shotgun sequence.
ACCESSION   DAOPUE010000102 DAOPUE010000000
VERSION     DAOPUE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12701_length_6152_cov_8.958504"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1277)
                     /locus_tag="PKH42_08190"
     CDS             complement(105..1277)
                     /locus_tag="PKH42_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20452.1"
                     /translation="MKQRYKRIVKVSVVLVIGFWVLSIIPFNQNIRQEVSVNIYENGI
                     VTEKTTIFVYGEKSNYLFHDDESFNGVYTLKGKEIIPPIYEYIDYYATDKYMIVNNGW
                     YADFVDKKTGEMILNNSDIHVPDIKYINDKYYAEGSSYAIYNLAGQKLTWQSYNDISI
                     ININDEDLLAAKYNTERFNTHFDYFKQTKGPSSWAVDEVSKAIKSNLVPFEFQAAFTF
                     NIKRYEFCSIIVEFLEEYYNTSREEIIKDKNIDVTNQAFVDGINEDIAICLHLGIVKE
                     RGNGIFDGDSEITREEAAVMLTNLANYLGMNTDTDEVKLYDKEKVSEWAIDAVNFVLK
                     NKFMQGVGNDMFSPKSNITREQTYIIMYRILNETEFYSLFDKASEAWVFSKFPAWW"
     gene            complement(1432..2250)
                     /locus_tag="PKH42_08195"
     CDS             complement(1432..2250)
                     /locus_tag="PKH42_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002087231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOA20453.1"
                     /translation="MKKYDLKPINRSKLRLLAGKAYYSLSRYLLWYSGKIKFAENQSK
                     QNLKYVIFSHKTPLLRKLRDVDMQYQYNKITNLKLAVGKIDSIIIKPGETFSYWKLIG
                     KTTARKGYVPGMVLHYGNYTKGIGGGLCQLSNLIYWMTLHTPLTIVERYRHSFDVFPD
                     SNRTQPFGSGATCVYPYRDLMIRNDTNEDYKLKVKVGQSYLEGSWYSNSKPIYKYEII
                     EKNHHFESEFWGGFSRHNELYRRCYTSEDVLLYEEYITENHALMMYSPFLDSSN"
     gene            2543..2992
                     /locus_tag="PKH42_08200"
     CDS             2543..2992
                     /locus_tag="PKH42_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOA20454.1"
                     /translation="MDFSQEDFNQALNLIKEAVQGERADELLYDYFINIAPSQEEKDI
                     IATIRDDERNHRKWFKDVYRYYTGEEIETRNGEEFIKPESYLAGISKAVFGELSAMEK
                     YRFIREGLPVRLFRDTVFRIITDEMKHATKYNYILNKNALKQTTSAM"
     gene            3349..3879
                     /locus_tag="PKH42_08205"
     CDS             3349..3879
                     /locus_tag="PKH42_08205"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOA20455.1"
                     /translation="MEQYSNKKFSLLEFNNLETHDENKYELIDGIVLMSPRPNYIHQQ
                     IMSRLNLEIGTYLKGKKCMVFSEVELEFDNNVIIPDLSIICGLNDFNFQRYKSAPELV
                     IEIISPGSQYTDTFTKLYKYELFGVKEYWIANPKTEIITVYNFENKTNKDYCKQENLI
                     SSVFNDLEINLSEIFL"
     gene            complement(3939..4250)
                     /locus_tag="PKH42_08210"
                     /pseudo
     CDS             complement(3939..4250)
                     /locus_tag="PKH42_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
     gene            complement(4546..5289)
                     /locus_tag="PKH42_08215"
     CDS             complement(4546..5289)
                     /locus_tag="PKH42_08215"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HOA20456.1"
                     /translation="MLKNKVAIVTGGTRGIGFAIVKKYLENGATVVLCGSKQETADIA
                     VKKLKDENADCKVEGIGLNLNSPEEIKKAFENIKTKYGRLDILVNNAGISARESIYEY
                     DPVNFEKIIDLNVNSVFYCTQAAVKIMKEQGGGSIINTSSMVSFYGQTSGCGYPASKF
                     AINGLTKSLARELGRDNIRVNAVAPGITNTDMVAALPDEIIKPLIATIPLGRVGEPED
                     VANAFLFLASDMSSYVTGAVLPVDGAARS"
     gene            complement(5521..>6152)
                     /locus_tag="PKH42_08220"
     CDS             complement(5521..>6152)
                     /locus_tag="PKH42_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HOA20457.1"
                     /translation="YISHLIHCIQMNYEYVAGGEQYNVRDKMMAENVLWVMEHEMKYY
                     DNENIILFAHNGHIMEDDFIMNYRDMLYITIENKDMLHITMGHHLSNYLGDDYYTIVT
                     EAKNNSFLADSNLPNDKRKLFSIERKGSLIDTIGVESPSIKFCTSEYLKQAGISTWDL
                     TFIGSYFNNLNTFIPARFTINTNVETCFDAMLYYDQLTPNIDNKSYLDK"
BASE COUNT         1999 a         1116 c          859 g         2178 t
ORIGIN      
        1 tcttaattct atcgaaatta actagctgaa caattccata aataattaca actatcgtta
       61 tcactattgc taaagacatt ggggtattac tgcgatatcc gattctacca ccaagcaggg
      121 aatttggaaa acacccatgc ctcacttgct ttatcaaata aagaataaaa ttcagtttca
      181 ttcagtatcc tatacataat aatatatgtt tgttctcttg ttatatttga tttaggagag
      241 aacatatcat tgccgacacc ctgcatgaat ttgtttttca gaacaaagtt caccgcatca
      301 atagcccatt ctgatacctt ttctttgtca tataatttta cttcatctgt atcagtattc
      361 attcctaaat agttcgccaa attagtaagc attacagctg cttcttctct tgtaatttcg
      421 ctgtcaccgt caaatatacc gttacccctt tctttaacta tacctaagtg gaggcagata
      481 gctatatctt cattaattcc atcaacaaag gcctgattgg tcacatctat attcttatcc
      541 ttaataattt cttcccttga agtgttataa tattcttcca aaaattctac tattatgctg
      601 cagaattcgt atctttttat attaaatgta aatgctgcct gaaattcaaa tggaaccaaa
      661 ttgcttttta tagctttact aacttcatct acagcccatg atgacggtcc ttttgtttgc
      721 ttgaaatagt caaaatgcgt attaaatctt tcagtattat attttgctgc taatagatcc
      781 tcatcattaa tatttataat tgaaatatcg ttataagact gccatgttaa cttctgacct
      841 gctaaattat aaatagcata gctgctgcct tctgcataat atttatcatt aatatatttt
      901 atatccggta catgtatatc agaattatta agaatcattt cccctgtttt tttatcaaca
      961 aaatcagcat accatccatt attcacaatc atatatttat cagtggcata ataatctata
     1021 tattcatata ttggaggaat tatttcttta ccctttagtg tataaacacc attaaatgat
     1081 tcgtcatcat gaaagagata gttacttttt tctccatata cgaaaatagt tgttttctcg
     1141 gttactattc cattttcgta tatatttaca gatacttctt gccttatatt ttggttaaat
     1201 ggaattatag aaagtaccca aaacccaata actaaaacca cactcacttt tacaatcctt
     1261 ttatatctct gtttcatttc tcttatacct ccaaatacta ataatatttg ctacgcactt
     1321 tagtgcttat gtgcattaac atattcggat aattacttgt aagctaagca aagaatttaa
     1381 tctcacctaa ggagtacgct cctggacgaa tctttataag tttcctaaga tttaattact
     1441 tgaatctaaa aaaggtgaat acatcattaa tgcatggttt tccgtaatat attcttcata
     1501 caataagaca tcttcacttg tatagcatct gcgatataat tcattatgcc tactaaatcc
     1561 cccccaaaac tcgctttcaa aatgatgatt tttctctatt atttcatatt tataaattgg
     1621 cttactgtta gaataccaac taccctccaa ataagactgt ccaactttga cctttagctt
     1681 ataatcttca tttgtatcat tcctaatcat tagatctcta tagggataaa cgcatgtagc
     1741 tccacttcca aaaggttgag ttcgatttga atcgggaaat acgtcaaaag aatgacgata
     1801 acgttcaaca attgttaatg gagtatgaag ggtcatccaa taaatcaaat tagaaagttg
     1861 acataatccg ccccctattc cttttgtgta attaccataa tgtaatacca taccaggtac
     1921 ataacctttt cttgctgtag tcttgccaat aagcttccaa tagctgaaag tttctcctgg
     1981 ttttattata atgctatcta ttttaccaac tgctaactta agatttgtaa ttttattata
     2041 ttgatattgc atatcaacat ctctgagttt tcttagtaat ggagttttat gtgaaaaaat
     2101 aacatacttt aaattttgtt tactttggtt ttcagcaaac ttaatctttc cagaatacca
     2161 cagtaaataa cgggaaagtg aataatatgc tttcccggct aataatctta gtttactgcg
     2221 attaattggt ttcaaatcat attttttcaa atcctcgccc tttatctatt gtttttttct
     2281 cattctcttt ttattttcat attttctatt agagcaatca tttctcttat tgattctcct
     2341 tgataatata agacgataaa tatagcaact tgttccaata ccaaatccat acttagaaaa
     2401 ttaagtttat cacttttatg tacatacaca aacaagttat ctttttcata tattgtttat
     2461 aaaatgataa ttaggggtga tttatttgta taactataac aggccgtata tgaacccaaa
     2521 cataaaatgt tcaacctatg gaatggattt ttcgcaagaa gattttaatc aggcacttaa
     2581 tcttattaaa gaagcggtgc aaggtgagag agcagatgaa cttttatatg actactttat
     2641 caacattgca ccttctcaag aagaaaagga tataattgcc acaataagag atgatgaacg
     2701 aaatcataga aaatggttta aagatgtata caggtattat accggggaag aaattgaaac
     2761 ccgaaatggc gaagaattta taaaaccaga atcgtattta gccggcatca gcaaagccgt
     2821 tttcggtgaa ttaagtgcaa tggaaaaata tcgtttcatt agagaaggac ttccggtaag
     2881 actcttccgt gatacagttt tcagaataat tactgatgag atgaagcatg cgactaaata
     2941 taattatata ctcaataaaa atgcattaaa acaaactacc tctgccatgt aagccttttg
     3001 gggttttgtc aagcaataaa atgcataaaa aaattatata atataaggat gtgcaaaaat
     3061 gcaatcatgt gaactaatag cttatatctc tgctattgca tgtattatct caaagagttg
     3121 ttccaaggat gaacttccta taatagcctc attttttact caattaggtg acacattaaa
     3181 tacaattatt gcaaatgaag gattttgttc taatgatgtg gaagaataat tccacatctt
     3241 tttactttac attccggcat aatcccacat tcaaaatata tattgttatt taatatattt
     3301 tttgatataa ttataaaaaa caacgtatat aaataattgg aggtatcaat ggaacaatat
     3361 tcaaataaaa aattttcatt gttagaattt aataatctag aaactcacga tgaaaacaaa
     3421 tatgaattaa ttgatggaat tgttttaatg tcacctcggc caaattatat acaccagcaa
     3481 attatgagca ggttaaatct tgaaattgga acttatctta agggtaagaa atgtatggtg
     3541 ttttctgaag ttgaattaga atttgataat aatgttataa ttcctgattt atcaattata
     3601 tgtggattga atgattttaa tttccaaaga tataaaagtg cccctgagct tgtaattgaa
     3661 attataagcc ccggcagtca atacacggac acattcacaa aactttataa atatgaacta
     3721 tttggggtta aagaatactg gatagctaat cctaaaactg aaattataac agtatataat
     3781 tttgaaaata aaactaataa ggattactgt aaacaggaaa atttaatatc ctctgttttc
     3841 aatgatttag aaattaactt gtccgaaatt ttcttataat attctggccc cttgccatgt
     3901 ccaccattaa gtgcctatca tagctctatt ttgatctgtt attcattcca actttttaac
     3961 gtatctaaat caaatgtcca ttggggtgtt attaattttt ctttgccatt tagtgaatac
     4021 catgggctta tatcatcatc cattggattt atgagaagtt gagtttgctg ggcgggcatg
     4081 taggactgtg ccagtaaaaa tattttctcc ccggtagaat taactgccat gtcaacaact
     4141 attacggcat ggccgggact gccgcccaca ataaacacat ctccaatatt catattatca
     4201 atactgacac ttataagctc attttccaaa gatagtgttc cggagtacgc atgccattaa
     4261 gttaaggcaa aaataagcta caacaaaaaa acctcactta ttctaaaaaa tgagggaggt
     4321 tttgatgctg taacatagtc agctcttctt tttcattcga ctccaaaagt aacccatgag
     4381 gaatagcata acttccatta ttctgaataa gcaggatata tttacattat ttcttcagac
     4441 ttacaagtaa ttccttacat cgttgataaa tatttattgg ctttcatggt ccttgtaata
     4501 gtcttaaaat ataatatata aaggcatctt tttgttttac tctaattaac ttctggcagc
     4561 accatccaca ggcaaaactg cacctgttac atagctggac atatcacttg ccaagaaaag
     4621 aaatgcatta gccacatctt caggctcacc tacacgtcct aaggggatag tagcaatcag
     4681 aggctttatt atttcatccg gcagcgcagc taccatatcg gtattcgtaa tacctggcgc
     4741 taccgcattt actcttatgt tatctcttcc aagctctctt gctaaggatt ttgttaatcc
     4801 gttaattgca aacttcgaag ccggatatcc gcagccggaa gtttgaccgt aaaaacttac
     4861 catggagctg gtatttataa tactgcctcc accttgttcc ttcattattt ttactgctgc
     4921 ttgagtgcaa taaaatacgg aattcacatt taaatcaatt attttttcga aatttacagg
     4981 atcatattca tagatgctct ctcttgccga tatacctgca ttgtttacta aaatatcaag
     5041 cctgccatat ttagttttta tattttcaaa ggcttttttg atttcttcag gtgaatttaa
     5101 atttagtcca ataccttcaa ccttacaatc cgcattttca tctttaagtt tcttaacagc
     5161 tatatctgca gtttcctgtt ttgaaccgca caatactaca gtagcaccgt tttctaagta
     5221 tttttttact attgcaaaac caattcctct ggtcccgccc gttacaattg caactttgtt
     5281 ttttagcatt tataaatccc tccattgaca ttatttggaa tgcatacttc actataatac
     5341 aattgatagt aaattgtcaa gcaattcgcc atacctgcag ccttgatgta aattgcaagt
     5401 ttaattgccc aatatttttt accagctatg cttaggttca tagggttctc tcaccctgag
     5461 gggaatatta attctcttat aatatactta ccccttgcca tgtccaacat aggttaaata
     5521 tcatttatcc aggtagcttt tgttatcaat attgggagta agctgatcgt agtacagcat
     5581 agcatcaaag catgtttcta cattagtatt tattgtaaaa cgggcaggaa taaaagtatt
     5641 taaattatta aagtaggatc caataaacgt taggtcccat gtggatatac ctgcttgttt
     5701 cagatattcc gatgtgcaga acttgatact tggtgactcc acaccgatag tatctattaa
     5761 tgagccctta cgctcaattg aaaacaattt tctcttatca ttggggagat tgctatctgc
     5821 taaaaaagaa ttgtttttag cttcagtaac aatagtataa taatcatcgc caagataatt
     5881 tgataaatga tgtcccatgg taatatgcag catatcctta ttttctatgg taatatacag
     5941 catatcccta taattcatta taaaatcatc ttccatgata tgaccgttat gtgcaaacaa
     6001 tataatattc tcgttatcat aatacttcat ttcatgttcc ataacccata aaacgttttc
     6061 agccatcatt ttatctctga cattatattg ctctccgccc gctacatatt cataattcat
     6121 ttgtatgcag tgtatcaggt gcgaaatata at
//
LOCUS       DAOPUE010000103         6136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12756_length_6136_cov_9.004111, whole genome shotgun sequence.
ACCESSION   DAOPUE010000103 DAOPUE010000000
VERSION     DAOPUE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12756_length_6136_cov_9.004111"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="PKH42_08225"
     CDS             <1..1358
                     /locus_tag="PKH42_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971736.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOA20458.1"
                     /translation="KILLVRPPRIKQAITLSDFMFSEPLGLEMIYGVLKEDHEVEIFD
                     MMIETDSLAVKIVEFKPELVGITSLCIDVIKVLKLCKETKEINSNIITAVGGTQAYLN
                     PEGFYNENVDYIFEYTTRDNINILFDSLKKEEKILIDGVRTRSLSYKSTGQKGRNEYM
                     LPDRSSTNKYRNEYSYFGYKPAAIMEFGTGCKKVCDFCLRWRIEGAEEKLIDIELTKN
                     DLRNIKEPTVMFIDNDFLCSEEKLNSFISIVKNLNFKKNYIMYGSVKGVIRYEKYLKE
                     LHEIGLKALLVGYETFNDDELLKYRKSSSTDDNLKASEILRKNKIDVWASFMAHPDWN
                     KNDFISLRKYIKKLRPQITSISPLTPFPNMPMYEEFEDRLLYQKEDFEKWSFGQVMIK
                     PSKISLREYYFELLKTNLYVNIFINKNTEMIKKFGIINIFRILKGSLKAVKKYVSLMY
                     E"
     gene            1443..1616
                     /locus_tag="PKH42_08230"
     CDS             1443..1616
                     /locus_tag="PKH42_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20459.1"
                     /translation="MDKNFEEIKEKFINASLEEKIKIYTTTQGLTVEQFKELLAYFPI
                     KYLDKLEEAVNSN"
     gene            1698..>2533
                     /locus_tag="PKH42_08235"
     CDS             1698..>2533
                     /locus_tag="PKH42_08235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOA20460.1"
                     /translation="MIKDNKLKKTYIKEALGNRNYLSYRTDLILLKILVSFAVFIATY
                     FIYLDLILSLLIASLVFFIFTLINKINVNKKNEKGREILIKKVKEEYFSSKIEEINQS
                     DFELLIKLFFKNEGYNNFIKKGRNLYLAEKDGYITCIKIFKLYGGIEVEKLDVRSLLT
                     FMGNSNIRNGFLVTTSGLSKDAAELIEKFKDKFEITIIDIEGMYNLADKYNMLPDESF
                     YYKKLSEEKTVTKKDFKENILNIKKIYLYIAASVFFYMTSVWMPENKLVIYISYYFII
                     LTT"
     assembly_gap    2534..2633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2634..3307
                     /locus_tag="PKH42_08240"
     CDS             <2634..3307
                     /locus_tag="PKH42_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA20461.1"
                     /translation="FNKLIEVARRTPTSNGMQASSIIRVTDNQKRKEIADVCKQEYVA
                     RAPELLILIADQYRNYRIADEKGGYEPSARDMDRFFQGFTDACLTAQNIVNAAEALDL
                     GVVYLGSILNDAEKIIEILELPELTMPVLGLGIGYPNQNPQLKPRMSMDLRLFENTYK
                     KFDDYLEEIKEYDIEMQTYYDLREANKRVDSFSNQVVTKLKNLIPKRSDMLKVMKKQG
                     FDLNI"
     gene            3463..4041
                     /locus_tag="PKH42_08245"
     CDS             3463..4041
                     /locus_tag="PKH42_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOA20462.1"
                     /translation="MRFKVTVGLSNKHVHLSKEHIDILFGEGYELTPIKDLTQPGQFA
                     CDEKVDLVGPKRTIKGVRILGPARKETQVELSFSDGFTLGITDLPVRDSGKTEGTPGL
                     KLVGPKGEVEIERGAIAAARHLHMHTTDAEKYGLKDKDIVSVKVDGQRGLVFDNVLVR
                     VNEAYALDMHVDIEEGNAAGLKNGDLVEVIVE"
     gene            4330..6126
                     /locus_tag="PKH42_08250"
     CDS             4330..6126
                     /locus_tag="PKH42_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOA20463.1"
                     /translation="MDFLPVNMEDMKKRGWDECDFVFVTGDAYVDHSSFAAAVLSRTL
                     EKFGYKVGIIPQPDWKELSSFKVLGKPRLAFLVNSGNIDTMVNHYTTFKKRRHQDAYS
                     PGGKAGLRPDRAVTVYSNRIREAYKDVPVIIGGIEASLRRFSHYDYWSDSVKRSVLLD
                     SGADLLVYGMGEKVLIEIAEALESGIPVEEITFINQTVYKTKDRERPYNPIFLPDYNE
                     VVSSKKKYGESFKIQYENTDSKSARTLVEPYNTVYVVQNPPMEILSQQELDDIYDMDY
                     MRDYHPMYKNLGVPAFNEVKFSITSNRGCFGGCSFCSLNFHQGRIIQARSHESILNEV
                     EKMKKDKDFKGYIHDVGGPTANFRIKACKKQEKYGACKNKECLTPQKCENLQIDHKDY
                     IELLKKLRSIEGIKKVFIRSGIRYDYAIYDNNDDFIEELCKYHVSGQLRTAPEHVSES
                     VLKLMGKPSIKIYNKFVEKFNDVNKKINKDQYIVPYFISLHPGSRLKDAIELAEYIRD
                     MGHIPEQVQDFYPTPGTLSTCMYYTGFNPLTNKEVYVPKDIEEKKMQRALLQYSKKEN
                     YELVLKALKKENRYDLIGYGPKALIKPRQKKH"
BASE COUNT         2259 a          770 c         1193 g         1814 t
ORIGIN      
        1 tgaaaatatt attggtaaga ccgccgagaa ttaaacaggc aataacactg agcgacttta
       61 tgttttccga gcccttgggt ttggaaatga tttacggtgt tttaaaagaa gatcatgaag
      121 ttgaaatttt cgacatgatg attgaaactg acagcctagc tgtaaaaata gttgaattta
      181 aaccggaact ggttggaatt acatcccttt gcattgatgt tatcaaggtt ttaaaacttt
      241 gcaaagaaac aaaagaaata aattcaaata taataacggc tgtgggggga actcaagcct
      301 atttgaatcc ggaaggcttt tataatgaaa atgtggacta tatttttgaa tatacaacaa
      361 gagacaacat taatattctt tttgactcac taaaaaaaga agaaaaaata ttgattgacg
      421 gagttaggac gaggtctttg tcatataaat caacaggaca aaaaggaaga aatgaatata
      481 tgctgcctga cagaagttca acgaataagt acaggaatga atattcatat tttggctata
      541 aacctgctgc aataatggaa ttcggcacag gctgtaaaaa ggtatgcgat ttttgtctca
      601 ggtggagaat tgaaggtgca gaagaaaaac tgatagatat tgagttaacc aaaaatgatt
      661 taagaaatat taaagagccg acagtaatgt ttattgacaa tgattttttg tgcagcgaag
      721 aaaagctcaa ttcatttatc agtattgtaa aaaacctgaa ttttaaaaag aattatatta
      781 tgtacggctc cgtaaaaggt gttatccggt atgaaaaata tttaaaggaa ttgcatgaaa
      841 tagggttgaa agctcttctt gtaggctatg aaacatttaa tgatgacgaa ttattaaaat
      901 atcgcaagag ctcatcgaca gacgataatc taaaggcttc ggaaatactg agaaagaaca
      961 aaattgatgt atgggcttct ttcatggctc atccggactg gaataagaat gattttattt
     1021 ctttaagaaa gtatattaaa aagcttagac ctcagattac ttccataagc cccttgactc
     1081 cttttcccaa tatgcccatg tatgaagagt ttgaagacag acttctttat caaaaagaag
     1141 attttgaaaa gtggagtttt ggacaggtga tgataaaacc ttcaaaaata agcttaaggg
     1201 aatattattt tgaattattg aaaacaaatt tatatgtcaa tatatttatc aataagaata
     1261 ctgaaatgat taaaaaattt ggaattatta atatatttag aattttaaag ggctctttaa
     1321 aggcagtaaa aaagtatgta tcattaatgt atgaataaaa aaaataaaaa attttcctat
     1381 aatacatttt gataattgtg aaataatata aaaatagaga aatttcaaag gaaaggtgta
     1441 ttatggataa aaattttgaa gaaataaaag aaaaatttat taatgcaagc ttagaagaaa
     1501 aaattaaaat ttatactaca acacaaggtc ttacggtaga acaatttaaa gaattattgg
     1561 catatttccc cataaagtat ttagataagc ttgaagaagc cgtaaatagc aattaaaata
     1621 tggcgaatgt ccctgtcaat tatccctgca gcgtataaat tacctcaatt tggaaataat
     1681 tccattgagg tgattttatg ataaaggaca ataaattaaa gaaaacttat ataaaagagg
     1741 cattgggaaa cagaaattac ttaagctatc gcacagattt aatactgtta aagatacttg
     1801 taagcttcgc agtttttata gcaacatatt ttatatactt ggaccttata ttatctttac
     1861 tgattgcttc cttggtattc ttcattttta ctttaataaa caaaataaat gtgaacaaaa
     1921 agaatgaaaa aggcagggaa attttaatta aaaaagtgaa agaggaatat ttctcatcaa
     1981 aaattgaaga aattaatcaa agtgattttg aattgctgat taaattattt ttcaagaatg
     2041 agggatacaa taattttatt aaaaaaggaa gaaacttata tttggcggaa aaggatggtt
     2101 atataacctg tataaaaata tttaagcttt atggcggtat tgaagtagaa aaacttgatg
     2161 tgagaagttt gttaaccttc atgggaaaca gcaatataag aaatggtttt ttggttacta
     2221 catccggatt aagcaaagat gcagctgaac tgattgaaaa gtttaaagac aaatttgaaa
     2281 taacaataat tgatatagaa ggtatgtata atttggcaga caaatacaat atgcttcctg
     2341 atgaatcatt ttattataaa aaattaagcg aagaaaagac tgttacaaaa aaagatttca
     2401 aggaaaatat tctaaatata aaaaaaatat atttgtatat tgcagcttca gtattctttt
     2461 atatgacgtc cgtctggatg cctgaaaaca aattagttat atatatttct tattatttta
     2521 ttatattgac aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgtttaa
     2641 taaattaatt gaagtagcta gacggactcc tacatcaaac ggaatgcagg caagctcgat
     2701 tattcgagtg accgataatc aaaaaagaaa agaaattgca gatgtttgta aacaggaata
     2761 tgtggcaaga gctccagaac tgttaatttt aatagcagac cagtacagaa attacagaat
     2821 tgcggatgaa aagggcggtt atgagccaag tgccagggat atggatagat tcttccaggg
     2881 ctttacggat gcttgcttaa ctgcgcaaaa tatagtaaat gctgctgaag ctttagattt
     2941 gggagttgta tatttgggaa gcattttaaa tgatgcggaa aaaattattg agattttaga
     3001 actgccggag cttactatgc cggtgcttgg actcgggata ggttatccga atcagaatcc
     3061 gcagcttaaa ccaagaatga gtatggattt gcgcttattt gaaaatacat ataagaaatt
     3121 tgatgattat ttggaagaaa ttaaagaata tgatatagaa atgcagacct attatgattt
     3181 aagagaagca aacaaaaggg tggacagctt cagcaatcag gttgttacaa agctaaaaaa
     3241 tcttattccg aaacgctcgg atatgctgaa ggttatgaaa aagcaaggat tcgacttgaa
     3301 tatataaggt ctgttttatt acacgaagac gattccctcg gtaggcgggg gaaaaaatat
     3361 ggtagttatg agcaataaca aattaaatta attaattgtt gtaattttaa ctattgtata
     3421 ttataatgct tttatatata cattaatttt acggaggatg ttatgagatt taaagttaca
     3481 gtcggtttat ctaataaaca tgtacatttg agcaaggaac atattgatat actgttcggg
     3541 gaaggctatg aacttacacc tattaaagat ttaactcaac cgggacagtt tgcatgcgat
     3601 gaaaaagttg atttggtagg acctaaaaga acaatcaaag gtgttaggat tctgggacct
     3661 gcaagaaaag aaacacaggt tgaattatct ttttctgatg gatttacatt aggtattact
     3721 gaccttcctg tgagagattc aggaaaaaca gaaggtactc cgggtcttaa acttgtggga
     3781 ccgaagggtg aagttgagat tgaaagaggt gctattgctg cagcaagaca tttacatatg
     3841 catacaacag atgctgaaaa atacggcctt aaagataaag atatagtttc tgttaaagta
     3901 gatggtcaga ggggtctggt tttcgataat gtattagtaa gagtaaatga agcatatgca
     3961 ttagacatgc atgttgacat tgaagaagga aatgctgccg gactgaaaaa cggggattta
     4021 gtagaagtaa tagtggaata gtttacataa aataatttat gtaaactccg accgttaatt
     4081 ctgtgcaagt atttggttct atatagtttg ttggatacat gaagatatat aacaattttg
     4141 ctctatgtaa gtgaattgga tacatggtag atgtttaaat gtctgaacag agtgttatta
     4201 tttagttcta tatagttatt gaatacatgt ttttatataa aagcagagga gtaattcttc
     4261 tgcttttatt attgttttaa atataatgca ggtggtataa taaacacatt aacattatat
     4321 ggaggcatta tggatttttt acccgttaat atggaagata tgaaaaaaag aggatgggat
     4381 gagtgtgatt ttgtctttgt gacaggtgat gcatatgttg accattcttc atttgccgct
     4441 gctgttttgt caagaacatt ggaaaaattc ggttacaagg tcggaatcat tccgcagcct
     4501 gactggaaag agttatcttc ttttaaagtg ttgggaaagc cgcgtttagc ctttcttgtt
     4561 aattcaggga atatagatac aatggttaat cattatacga cttttaagaa aagaagacat
     4621 caagatgctt attctccggg cgggaaagca ggattaagac ctgacagagc tgttacggta
     4681 tattcaaacc gtataagaga agcatacaag gatgttcccg taataatagg cggtattgaa
     4741 gcgagcttaa gaagatttag ccattatgac tattggagcg acagcgttaa aaggtctgtt
     4801 ttattggatt caggtgcgga tttattggta tacggcatgg gtgaaaaagt tttgatagaa
     4861 attgcggaag cattggaatc cggtattccg gttgaagaaa ttacatttat aaaccaaacc
     4921 gtatacaaga caaaggacag agagcgtccc tacaatccga tatttttgcc tgactataat
     4981 gaagttgtat cttcaaagaa aaagtatggt gaaagcttta aaatacaata tgaaaatacc
     5041 gactccaaaa gtgcgaggac cttagttgag ccgtataata cagtctatgt ggtacaaaat
     5101 ccccctatgg aaattctatc acagcaagaa ttagatgata tttatgacat ggattatatg
     5161 agggattacc atcctatgta taaaaacttg ggagtacctg catttaatga agtaaaattc
     5221 agcattacaa gtaacagagg atgcttcggt ggctgcagct tttgctccct taatttccat
     5281 cagggaagaa taatacaagc aagaagtcat gagtccatat taaatgaagt tgagaagatg
     5341 aagaaagaca aggactttaa aggatatatt catgatgtgg ggggacctac tgctaatttc
     5401 agaattaagg cttgcaaaaa acaggaaaag tacggtgcgt gcaaaaataa agaatgttta
     5461 actccgcaaa aatgcgaaaa tttacagatc gaccataaag attatataga attattgaag
     5521 aagctgagaa gtatcgaagg tatcaaaaaa gtttttattc gttcaggcat aagatacgat
     5581 tatgcaatat atgataataa tgatgacttt attgaagaat tatgcaaata ccatgtgagc
     5641 ggacaattaa gaaccgctcc ggaacacgtt tcagaatcag ttttaaaact aatgggcaaa
     5701 ccttcaataa aaatatacaa taagtttgta gaaaagttta atgatgtaaa caaaaagatt
     5761 aacaaagatc aatacattgt accttatttt atatcattgc atcccggttc tcgcttgaaa
     5821 gatgcaattg agcttgctga atatataagg gatatgggtc atattcccga gcaggtacag
     5881 gatttttatc ctacaccggg gacactgtca acatgcatgt attatacggg ctttaaccca
     5941 ttgacaaata aagaagttta tgttccaaaa gatatagaag aaaagaaaat gcaaagagca
     6001 ttgcttcaat attccaaaaa agaaaattat gagctggttt tgaaagcttt aaaaaaggaa
     6061 aacagatatg atttaatcgg atacggtccc aaagctttaa taaagccaag acaaaaaaag
     6121 cactaaaggt gcctgg
//
LOCUS       DAOPUE010000104         6111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12827_length_6111_cov_9.738111, whole genome shotgun sequence.
ACCESSION   DAOPUE010000104 DAOPUE010000000
VERSION     DAOPUE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12827_length_6111_cov_9.738111"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..4997
                     /locus_tag="PKH42_08255"
     CDS             132..4997
                     /locus_tag="PKH42_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HOA20464.1"
                     /translation="MNRSVLKELQKEILLNLTQNEFLKSLSVSENKIQSYLNNSKFTK
                     GLSTLFNKKQITCKAVLDLSADILNNEQVEPPENWLSYIFQYVLEKSFPDAVTIELNS
                     EYEISAVIYLEILRTILKYTQINNIGKVPKFEFLTEEEINDLSNKQEYLNFLDVFDKN
                     YIYELMMLDGEVNGYNTLHHVSTVHFVAMHVAKQLKKAGVQVDLGIVSGSAAGHDIGK
                     YGCKGLEKRRVAYLHYYYTDQWFKKYDMPGIALIAANHSTWDLELENLSLESLLLIYS
                     DFRVRNKITKTGEEMQIFSLAESFDIILKKLDNVDEAKEKRYIRVYSKLRDFEDYLLN
                     KGINTDLLSEEPKYVKTVDFALIDGYEVVKNFKFKAFEHNIPLMRMLNNEVTFTGMIE
                     AARSEWDWKNIRAYLNILEEYSTYLSQKEKLFALSFLYELLVHREGDIRRQAAKLMGT
                     IIIHYDMGYTKEMPEDVKITHKEKNAGLSLWDKYLGFFLTPGYKVTDKQKEWIGYSLR
                     MFVDSVINSPRNTLKEEYLEIFLKHIREDINDETARFNSLNSLLSIPLELYDKEQLDF
                     VVDFSIKHFRDTSYSIRLMAAQFLFKAVQQIKITGHTLKEILNIVSEFSPDDGLCMNY
                     LKYKTAQCLNVPGTLLKKYSSLLAGNWYKTSDIFLNNLKAATPWNVKTVSIDYIMENL
                     SQRNELALLQTATHLANLVKVSAMESVRNKAGNSLVQLGPMLTIDQRNEIAFELIKGL
                     EIDEMQYAKYIPEYLGRFVMLLSPKELDEFIIDLKNIYINSSERSSALVIHTFGIMVQ
                     YYPEYKERFGEDSSVIEKRLIKILGIILGGLANFNTQVKQETFLVIGQYIFGSKILTL
                     KQKHKVFSLIYKKLLTLISEKELSELFFFNNSASFNHIYRFISDYEFFNGKFDIKENK
                     NIAFFPGTFDPFSLSHKGIVKEIRNLGYDVYLAVDEFSWSKKVQPRLIRRQIINMSIA
                     DELGVFLFPDDVPVNLSNNKDLKILKTLFPKKDIYIVVGSDVLINATAYNNEPEEDSI
                     HNFNHIVFKRAKDEITDEAVKKAEEAKKRIIGTLVELKLPVYLEDISSTQIRENIDNN
                     RDISNLIDPMAQNFIYDRNLYIREPLNKAVLRTKPFVIEIVKELSKKILDEIDHCIFN
                     DTKLFENIAEKLNFKNIRLLVIRDSKNYNEMLGFSAFHKISTSDVYSEFKSPNIANYV
                     REITSGRIIVIDGIFAAPGRIYDSMEQTLITETLSHCIKNDFTYVLYNNIITGFDSDE
                     LLETLKLQGFAKIHDKSTGKIVYGVDMKFPICLTFNLESFIKEPLNENKNVYEAISYS
                     RKRLQRAMTQLYPGSLVLSFDNDMINQILINKICSLNNVPNEMQEPRVLGEYMVVPFG
                     NVLKGMIVPNTVTKSLHTEKVYSSDATRFKIKEYPFYSSIENQIRTIKSFEKPVILVD
                     DLLHKGYRIKEIDPILKRYNINVKKIIVGIMSGRGKDLKDTQGRDADYAYYIPNLRLW
                     FNENLMYPFLGGDGIMSENENITNLIPSINLLLPFYSPMYIRGASKEAIYNLSMACLE
                     NAKHILLALEKEYKEIFERNLTVKRLGEVLLSPRLPYLGDNIYYDLNKEASGYMDVNI
                     ETLLKLERIIK"
     gene            4997..>6111
                     /locus_tag="PKH42_08260"
     CDS             4997..>6111
                     /locus_tag="PKH42_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOA20465.1"
                     /translation="MFYYKYNDKYLFSIKDEYSFTKINSIPHDCQELYYLNNLTGNSK
                     RAFSVNHPSDLYINEESLDMLRVNDISYDLPENITELIDKRKIKAVNTAYSDYADCFY
                     MDEFNKRKVNILALGDVGSTLLIGLKLLGKDSISSIGIYDRSLDKIKRWEYEMNQTSF
                     PFDDSLPTVQGINKEELFDCDMFIFCASKGVPPVGSQVADVRMAQFEGNAELIKEYAL
                     MAADCRFKGIFAVVSDPVDQLCQTVLKESRGVLAPEQIKGYGLGVMNARALYYSKKFN
                     KFSMYLKEGRAFGPHGKDLIIANSIRDYDDELSRELTKYAVEANLEVRKTGFKPYIAP
                     ALSSGAISIIYTLEGKYHYSSNYLAGVYMGAKNRNLPS"
BASE COUNT         2183 a          921 c         1188 g         1819 t
ORIGIN      
        1 gacacacctc taaagtaggg ataggctttg gggtaagctt tagaggaatg tccccgttag
       61 tataactttc aattgcagta ccaatgagag tatgttataa tttcgtaaat taaattaaaa
      121 gggaggttac aatgaacagg tctgtactta aagaattaca aaaagaaatt ttacttaatc
      181 ttactcaaaa tgaatttttg aaaagcctta gcgtttccga aaataaaata caatcttatc
      241 tcaataattc aaaattcacg aaaggcttat caaccttatt taataaaaaa caaataacct
      301 gtaaagctgt acttgacctg tcagcagata ttttaaacaa tgagcaagtt gaacctcctg
      361 agaactggtt atcatatata tttcaatatg ttttggaaaa gtcctttccg gatgccgtta
      421 caatagagct gaattccgaa tatgaaattt cagctgtcat atatcttgaa atattgagaa
      481 ctattttaaa atatacgcaa ataaacaata tcggtaaagt tccgaaattt gaatttttaa
      541 ctgaagaaga aataaatgac ctttcaaaca agcaggaata tttgaatttt ttggatgtgt
      601 ttgataagaa ttatatttat gaacttatga tgcttgacgg ggaggtcaac ggttacaata
      661 ctttgcatca tgtttcgaca gtacattttg ttgcaatgca cgttgcaaag cagcttaaga
      721 aggccggagt ccaggtggac ttaggcattg tttccggctc tgccgcaggc cacgacattg
      781 gaaagtacgg ttgtaaggga ttagaaaaaa gaagggttgc atatcttcac tattattata
      841 ccgaccagtg gtttaaaaag tacgatatgc cggggattgc tttaattgca gcaaatcatt
      901 ccacttggga tttggagctt gaaaacttat ccctggaatc attattgtta atatattctg
      961 attttagagt aaggaacaaa ataaccaaga ccggtgaaga aatgcaaatt ttttcattgg
     1021 cggaatcatt tgacatcatt cttaaaaagc ttgacaatgt agatgaagca aaagaaaaaa
     1081 gatatataag agtatattcc aaactaaggg attttgaaga ctatttatta aacaaaggaa
     1141 taaatactga ccttttatcg gaagagccta agtatgtaaa gacagttgac tttgccctaa
     1201 tagacggata tgaggtggtg aaaaatttta aatttaaggc gtttgagcac aacattccct
     1261 taatgagaat gcttaacaat gaagttacat ttacaggaat gattgaggct gccagaagcg
     1321 agtgggattg gaaaaatatc cgtgcatatt taaatattct ggaggaatac tccacatatt
     1381 tgtctcagaa ggaaaagctg tttgctttaa gtttcctgta tgaactattg gttcacaggg
     1441 aaggagatat aaggcgtcaa gcagcaaaat tgatgggaac aattattatt cattatgata
     1501 tggggtatac aaaggaaatg cctgaagatg tgaaaataac acacaaggag aaaaatgcgg
     1561 gtttatcctt gtgggataag tatttgggat ttttccttac tcctgggtat aaagtaacgg
     1621 acaaacaaaa agaatggatt ggttattctt taagaatgtt tgttgattca gtgataaact
     1681 ctccaagaaa cacattaaag gaagaatacc tggagatatt tttaaaacat atccgtgaag
     1741 atataaatga cgaaaccgca aggtttaaca gtttaaactc tcttttaagc attcctttgg
     1801 agttatatga taaagagcag cttgactttg tggtggattt ttcgataaag cattttagag
     1861 atacatcata cagcataagg ctgatggcag cacaattttt attcaaggct gtgcaacaga
     1921 taaagattac gggacatact cttaaggaaa tactcaatat cgtatcagag ttttctcccg
     1981 atgacgggct gtgcatgaat tatttgaaat acaagacagc gcaatgttta aatgtacccg
     2041 gtacattact taaaaaatat tcatctcttt tagcaggcaa ttggtataaa acatcggata
     2101 tatttttaaa taatttgaaa gctgcaacac cctggaatgt aaaaaccgta agtattgatt
     2161 acataatgga gaatctttcc caaagaaacg aacttgcgct tctccagacg gcaacacacc
     2221 ttgcaaatct tgtaaaggtt agtgccatgg aatcagtgag aaacaaggcc ggtaactctc
     2281 ttgtacaatt aggtcccatg cttaccatag accaaagaaa tgaaatagca tttgagctta
     2341 ttaaaggtct tgaaatagac gaaatgcagt atgcaaagta tattcccgaa tatttggggc
     2401 gctttgtaat gttgttgtct ccaaaagagt tggatgagtt tattattgat ttaaaaaata
     2461 tttacattaa ttcaagcgag cgctccagtg cattggtaat acacactttc ggaataatgg
     2521 ttcaatatta ccccgaatat aaggaaagat ttggagaaga cagcagcgtt atagaaaaaa
     2581 gactcatcaa aattttaggg ataattttgg gagggcttgc taattttaat acacaggtaa
     2641 aacaggaaac atttttggta atagggcagt atattttcgg ctctaaaata ttgactctaa
     2701 agcagaagca taaggtgttt tcacttattt ataagaagct tttaacatta ataagcgaga
     2761 aggaattaag cgagctgttc tttttcaata actccgcctc atttaaccac atatacaggt
     2821 ttataagcga ttatgagttt ttcaacggta aattcgatat aaaagaaaat aaaaatattg
     2881 cgtttttccc cggaaccttt gacccctttt cgctgagtca caagggaatc gtaaaggaaa
     2941 taagaaattt aggatatgac gtatacttgg ctgtagatga attttcctgg tctaagaagg
     3001 ttcagcccag attgataaga agacaaatta tcaatatgtc aattgcagat gaattaggtg
     3061 tatttttgtt tcctgatgat gtacccgtaa atttatcaaa caataaagat ttaaaaatac
     3121 taaaaacact ttttcccaaa aaggatattt atatcgttgt aggaagcgat gtattaataa
     3181 atgcgacagc atataataat gaacccgaag aagattcaat acataatttt aatcacatcg
     3241 tatttaagag agcaaaggat gaaattaccg atgaagccgt taaaaaggca gaggaagcaa
     3301 agaaaagaat aataggaaca cttgttgaat taaagcttcc tgtttattta gaggatataa
     3361 gctcaaccca aataagggaa aatatagaca ataacagaga tatatcaaac ttaatcgacc
     3421 ccatggctca aaatttcata tatgacagga atttgtacat cagggagcct ttaaacaagg
     3481 cagtactgag aacaaagccc tttgttattg agatagttaa agagttgagc aaaaaaattc
     3541 ttgatgaaat tgaccattgt attttcaatg atacaaaact gtttgaaaat attgcagaaa
     3601 agcttaattt caaaaatatt agactgcttg taataaggga ttccaaaaat tataacgaga
     3661 tgcttggatt ttctgcattt cataaaataa gcacctctga tgtttattcc gagtttaaaa
     3721 gtcccaatat tgcaaattat gtgagagaaa taacatccgg aagaattatt gttattgacg
     3781 gaatatttgc ggctcccggc aggatatatg acagtatgga gcaaaccctt attacggaaa
     3841 ccttatctca ctgcataaaa aatgatttta cctatgtctt atataataat attattaccg
     3901 gcttcgacag cgatgaattg ttggaaacat taaaacttca aggatttgcc aagattcatg
     3961 acaaaagcac cggaaagatt gtttacggag tagatatgaa gtttcccata tgcttgacat
     4021 ttaatctgga aagttttatt aaggagccct tgaatgaaaa taaaaatgtt tatgaagcta
     4081 tttcttattc gagaaaaagg cttcaaagag caatgacaca gctgtatccc ggaagtcttg
     4141 tattgtcatt tgacaatgat atgataaatc aaattcttat taataaaata tgcagtttaa
     4201 acaatgtgcc caatgaaatg caagaaccga gagtattggg cgaatacatg gttgtgcctt
     4261 tcggcaatgt tttaaagggt atgattgttc caaacacagt aactaaatca ttgcatacgg
     4321 aaaaagtata ttcttccgat gcaactagat tcaaaattaa ggagtatccg ttttactcat
     4381 ctatagaaaa tcagataaga accataaagt cttttgagaa accggtgatt ttggttgatg
     4441 atttacttca taagggttac aggattaaag aaatagaccc tatattgaag cgttataata
     4501 taaatgtcaa aaaaatcatt gtgggtataa tgtccggcag gggaaaggat ttgaaggata
     4561 cacaaggcag agatgcagac tatgcttatt atatccctaa cttaaggctt tggtttaatg
     4621 aaaatttgat gtatcctttt ttgggaggag acggcataat gagtgaaaat gagaatataa
     4681 caaatttgat tccttcaata aatttattgc tgccctttta ttcgcccatg tatataaggg
     4741 gagcttccaa agaagcaata tacaatcttt ctatggcctg cctcgaaaat gcaaaacaca
     4801 ttcttcttgc attggaaaag gagtataagg aaatttttga aaggaacctg actgttaaaa
     4861 ggctgggtga agttcttttg tcaccgagac tgccctactt gggagacaat atctattacg
     4921 atttaaacaa ggaagcatcc ggatatatgg atgtaaatat tgaaacactt ttaaaacttg
     4981 aaaggattat aaaataatgt tctattacaa atacaatgat aaatatttat tctcaataaa
     5041 agatgagtat agttttacaa aaataaatag cattcctcat gactgccaag aattatatta
     5101 tcttaataac ttaaccggaa attcaaaacg cgctttttcc gttaaccatc cttccgactt
     5161 gtacataaat gaggaaagcc tggatatgct tagagtaaat gacataagtt atgaccttcc
     5221 cgaaaatatt actgagctga tagacaaaag aaaaattaag gcagtcaaca ccgcctacag
     5281 tgattatgct gattgcttct atatggatga atttaataaa aggaaggtaa atattcttgc
     5341 cctcggtgat gttggaagta cattacttat tggacttaaa cttctcggaa aagattcaat
     5401 aagctccatc ggtatttatg acagaagttt ggataaaata aaaagatggg aatatgaaat
     5461 gaatcaaaca tcatttcctt ttgatgattc gctgcccacg gttcagggta taaacaagga
     5521 agaattattt gattgtgaca tgtttatatt ttgtgcttcc aagggggttc ctcccgtagg
     5581 aagccaagtt gccgatgtta ggatggctca atttgaagga aatgcagagc ttattaagga
     5641 atatgcctta atggcggcag attgccggtt taagggaata tttgcagtag tttcggaccc
     5701 tgtagaccaa ttatgccaaa cagttctaaa agagagcagg ggtgttttgg cacccgagca
     5761 aattaagggg tacgggcttg gagtaatgaa cgcgagagct ttgtactact cgaaaaaatt
     5821 caataaattt tcaatgtatt taaaagaagg aagagcattc ggaccgcacg ggaaagactt
     5881 gataatagca aacagtataa gagattatga tgatgagctt tcaagagaat tgacaaaata
     5941 tgcggttgaa gcaaacttgg aagtaagaaa gacgggattt aagccatata ttgcacctgc
     6001 cctttcatcc ggagcaattt caataattta tactctcgaa gggaaatatc attacagctc
     6061 caattattta gccggagttt acatgggagc taaaaacaga aacctgcctt c
//
LOCUS       DAOPUE010000105         6102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_12848_length_6102_cov_8.238135, whole genome shotgun sequence.
ACCESSION   DAOPUE010000105 DAOPUE010000000
VERSION     DAOPUE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12848_length_6102_cov_8.238135"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PKH42_08265"
     CDS             complement(<1..875)
                     /locus_tag="PKH42_08265"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOA20466.1"
                     /translation="MKNLLIVESPAKAKTIRTILGSNYTVKASVGHVRDLPKSKLGID
                     IENNFEPSYITIRGKGDIIQELKKEAKKADNVFLATDPDREGEAISWHLAKILNLDEN
                     KEIRIEFHEITKDAIKNASKAPRKLNIDLVNAQQARRVLDRLVGYKISPLLWKKIKKG
                     LSAGRVQSVALKLVIDREKEIMDFKPEEYWTLELEIKKSRKKFIAKYVGYFENDKLIK
                     VKINSQGEINKILESIDKEKITVAKIEKSKSYSKPGPPFTTSSLQQEANRKLNFTSKK
                     TMMVAQQLYEGVKVKG"
     gene            complement(1065..2612)
                     /locus_tag="PKH42_08270"
     CDS             complement(1065..2612)
                     /locus_tag="PKH42_08270"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOA20467.1"
                     /translation="MSVATYLTWHNLFEFLSIIASFSIFIVTYYIYEESGNLRMIILA
                     CSFFTMAFIDTFHTLSFKGMADFFVANLTANRATTFWILSRLLGSMGFMISSFIPEYK
                     RSELNKWVFIIPTSILVISLLIVVTYIPDFIPAMYVENYGLTETKIILEYLVVIIMII
                     AFLKIYREYKRNNKRLDYLIMIALLLSIFSELAFISYGSVYDAYNYLGHIYKVIAYFF
                     LFKAIYAESVSIPYRELKKAKNELKDYADNLNLLVKERTKELENMNEILMNDIESARL
                     MQRSLLPEKLPESQTVTFYAAYIPAERLSGDFYNVIKLDENNIALYIGDVAGHGVSAA
                     MLTVFANQNIKPIKEGEIFEIISPGYVMKSLYKSFNETHYKIESYLVMLYGIYNTKSR
                     QLTYTSAGINVPPLIIKSTGEILEMKIEGFPICKLGNFYMPYFEDISIQLNVGDIVLF
                     YTDGLVEYSNENGIMYSHNDLKDFLIKHYSMKASELYSAIKENLFKKIGNKKIADDVT
                     FLIMEIY"
     gene            complement(2870..3490)
                     /locus_tag="PKH42_08275"
     CDS             complement(2870..3490)
                     /locus_tag="PKH42_08275"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA20468.1"
                     /translation="MKKVLTIVLVFALSFGFLTIVNNMGNTDTASEQQTLTGTAEGFG
                     GDINVTVVVNGEDIISVEVTADGETQGIGSMAIDELPALIVEADSAEVEGVSGATYTS
                     DGIKAAVRNALSSKQGSEEEVTLTGTAEGFGGDVTVNVVVKGDDIISVEAIGEDETEG
                     IGSIALEELPALIAEADSTDIDGVSGATYTSDGIKAAVKNALESRQ"
     gene            complement(3706..4392)
                     /locus_tag="PKH42_08280"
     CDS             complement(3706..4392)
                     /locus_tag="PKH42_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOA20469.1"
                     /translation="MKTLYEGKTKKVLKDEKDNSVYIFFKDSATGENGVFDPGSNTVG
                     GIVEGKGKIGLIISKYFFELMEKNGIPTHYLGADIENGLMKVRNLTVPKLEFVLRYFT
                     AGSMCRRFSLDEGIPFDPPYAEVTLKDDEQGDPLISERLCIMKGLLSEGQYDEALRLL
                     EKVGEVLKKELAQMALTLIDFKIEIGYDESGKMYVVDEITPDIWRVRDKNGNIPNQIE
                     CSKLILNKIN"
     gene            complement(4419..4868)
                     /gene="smpB"
                     /locus_tag="PKH42_08285"
     CDS             complement(4419..4868)
                     /gene="smpB"
                     /locus_tag="PKH42_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOA20470.1"
                     /translation="MADKIVANNKKAFHEYFIEDKYEAGLVLVGTEVKSIRQGKVNLK
                     DSYVSIKNGEAFVYNMHISPYEKGNIYNVDPLRPRKLLLNKRELRKLAGLTTLKGYSL
                     IPLSLYLKNGLVKMELSVAKGKKTYDKRQDIAKKDAERRMQRQDDYR"
     assembly_gap    4915..5014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5015..5454)
                     /gene="pyrF"
                     /locus_tag="PKH42_08290"
     CDS             complement(<5015..5454)
                     /gene="pyrF"
                     /locus_tag="PKH42_08290"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOA20471.1"
                     /translation="MGKDVIIACDFSNRKEVVNFLSNFTEEKPYVKVGMELFYAEGPD
                     ILKLIKSMGHKIFLDLKLHDIPNTVRKAMKVLSEYNVDMVNIHAAGTIAMMEAAIEGL
                     TRKEGSRPILVAVTQLTSTDEERMKHEILIDKPMEEVIMHYAKCA"
     gene            complement(5497..>6102)
                     /locus_tag="PKH42_08295"
     CDS             complement(5497..>6102)
                     /locus_tag="PKH42_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HOA20472.1"
                     /translation="ILLSFIAEPVFVGIAYDAGGVASGPMTATFVLAFAQGAASSIET
                     ANVLVDGFGVIAMVAMAPVFSIMLLGTVFRYKKVEEYTPTEEEFVITPSIPEENRLLY
                     DCIMVNVERGFAENVVDLARQSGASGATIMHGRGTDIHHKVMLPIINVELQQEKEIII
                     FITKSNSTEKVADRLLSDKKLKEEGDIVIYICPVADAMIKY"
BASE COUNT         1774 a         1178 c          832 g         2218 t
ORIGIN      
        1 cctttgactt ttactccttc ataaagctgt tgggcaacca tcattgtttt tttggatgta
       61 aaattaagct ttctgtttgc ttcctgttga agcgagcttg ttgtaaacgg agggccgggt
      121 tttgagtagc tcttggattt ttcaattttt gctacggtaa ttttttcttt atcgatactt
      181 tcgagtattt tatttatttc accctgacta ttgattttaa ctttgattaa tttatcattc
      241 tcaaaataac ctacatattt ggcaataaat ttcttccttg atttttttat ttcaagctcc
      301 aatgtccaat attcttcagg cttgaaatcc attatttcct tctctctgtc tataaccaat
      361 tttaatgcaa cagattgaac tcttccggca cttaaacctt tctttatttt tttccataac
      421 agagggctta tcttataacc taccaatctg tctagaacac gtctggcttg ttgagcatta
      481 accaaatcta tattgagctt tcgtggagct ttgcttgcat ttttaattgc atcctttgtt
      541 atttcatgaa attcaattct tatttcctta ttttcatcca agtttaatat tttagccaaa
      601 tgccaagata tagcttcacc ttccctgtca gggtctgttg caagaaacac attgtcagct
      661 ttttttgctt cttttttcag ttcttgaatt atatcccctt ttccccttat tgttatataa
      721 cttggctcaa aattattttc tatatctata cccagcttgc tcttgggtaa atctcgcaca
      781 tgtcctaccg aagctttaac agtgtaattg cttcctaata ttgtacgtat agtcttagct
      841 ttggcgggag actctacaat taataaattc ttcatgtttc ctccattgat taggcaaatt
      901 tatatcccgt gcttattcac cttataatac aattttttat ttgtcaatag tttatgattg
      961 ttaacatatt aaaatagcca tcatatatga tggctagact gattatttat tatacaacga
     1021 ctcatgagat ccttgcttac gctcgggata acatgtattg ttgctcaata tatttccata
     1081 attaaaaatg ttacatcatc ggcaattttt ttattaccta tttttttgaa taaattttcc
     1141 tttatggccg aatataattc cgatgctttc attgaataat gtttaatcag aaaatctttc
     1201 aaatcattat ggctgtacat aataccattt tcattgctgt attctaccaa accgtcagta
     1261 tagaataaaa ctatatcacc gacatttaat tgaatactta tatcttcaaa atatggcatg
     1321 taaaagttcc caagtttaca tatgggaaac ccttctattt tcatttccaa tatttcaccg
     1381 gtacttttaa ttattaaggg aggaacattg attcctgctg aagtatatgt aagttgtcgg
     1441 cttttagtat tgtaaatgcc gtaaagcatt acaagatatg attcaatttt ataatgtgtt
     1501 tcattgaaag acttgtaaag acttttcata acgtaaccgg gagaaattat ttcaaatatt
     1561 tcgccttcct ttataggctt aatattttga ttggcaaaaa ccgtaagcat ggcggcagat
     1621 acaccgtgtc ctgcaacatc tcctatatat aatgcaatat tgttctcatc tagcttaata
     1681 acattgtaaa aatcaccgct cagtctttca gcaggtatat aggctgcata aaacgtaact
     1741 gtttggcttt caggcagttt ttcaggaaga agcgagcgct gcattaacct tgcactttct
     1801 atgtcattca ttaatatttc attcatattc tcaagctctt tcgttctttc tttaacaagg
     1861 agattaagat tgtctgcata atcctttagt tcatttttag ctttttttaa ttctctatat
     1921 ggaattgaca cgctttcagc ataaatagcc ttgaatagga aaaaatatgc tattactttg
     1981 tatatatggc caagatagtt atatgcgtca tatacgcttc catagcttat aaaagcaagc
     2041 tcactgaata tagacaatag aagtgctatc ataataagat agtctaacct tttattattt
     2101 cttttgtact cccgataaat ctttaaaaaa gcaataatca ttattataac aactagatac
     2161 tccaaaatta ttttggtttc tgtaagtccg taattctcaa catacatggc aggaataaaa
     2221 tccggtatat atgttacaac aattaacaag ctaatgacta atatagatgt tggaattata
     2281 aaaacccatt tatttagctc acttctttta tattcaggaa tgaatgaaga aatcataaaa
     2341 cccatacttc ctaataatct tgaaagtatc caaaaggtag tagctctgtt ggcagttaaa
     2401 tttgccacga aaaaatctgc cattccttta aagcttaatg tatgaaatgt atcaatgaaa
     2461 gccattgtaa aaaaagagca ggcaagtatt atcatacgaa gattgccgga ttcttcatag
     2521 atgtaatatg taactataaa tattgaaaac gatgcaatta tactcaaaaa ttcaaataaa
     2581 ttatgccacg ttaaataagt cgctaccgac ataatctcag taaatacatc gttataatat
     2641 gctgaagtta taaataacag aagtgaagat acgataataa ggataatttc tatatatata
     2701 ttattcttct tcttaatatc cgtcatctcc tttcaataca gctaatggag atttttgtag
     2761 atttttgtag atattttaat tatagtatac aatcaatatt tataccagta ttttttaata
     2821 taaaaaagct ataccaagat agtatagctt cttacccgat tcttaaggat tattgcctgg
     2881 attctaaagc attttttacg gcagctttta taccgtcgct tgtataagta gcacctgata
     2941 ctccgtcaat atctgtcgaa tctgcttctg ctattagggc aggaagttct tctagtgcaa
     3001 tacttcctat accttctgtt tcgtcttcgc ctattgcttc aacagatatt atatcatcgc
     3061 cttttacaac cacgtttaca gtaacatctc cgccaaaacc ttcagctgta cctgttaatg
     3121 ttacttcttc ttcagaacct tgcttagatg ataaggcatt tctaacagca gcttttatac
     3181 cgtcgcttgt ataagtagct cccgatactc cttcaacctc tgccgaatcc gcttctacta
     3241 tcaaagcagg aagctcatca attgccatag accctattcc ctgtgtttca ccgtcagctg
     3301 ttacttctac tgaaattata tcttcaccgt tgacaactac cgtaacattt atgtcaccgc
     3361 caaaaccttc ggctgttcct gttagtgttt gttgttctga tgcagtatct gtattaccca
     3421 tattgttaac tattgttaaa aatccaaaag acaatgcaaa tactaagacg atcgttaata
     3481 cttttttcat tatttcctcc aattactata catcgataaa tatttatttt ttcataacat
     3541 taaaaattat acaacaattg tatggccgtg taaagaagaa taatattcct aaatacatgt
     3601 aatttatacc cataaaaatt tttatgaaac atatatcttg attttcttta tattaaaaac
     3661 cgcttatttg ataagcggtc ttatttatca tttaaaaaat ctctattaat ttattttgtt
     3721 taaaataagt ttgctgcatt caatttgatt tggtatattg ccattcttgt cacgaacacg
     3781 ccagatatcg ggtgttattt catctactac gtacattttg ccgctttcat cgtatcctat
     3841 ttctatttta aagtcaatta atgtaagagc catctgtgcc aactctttct taagaacttc
     3901 tccaaccttt tctaataatc ttaatgcttc atcatattgt ccttctgata aaagaccttt
     3961 cataatacac aatctttcgc tgattaaggg gtctccctgc tcatcgtctt tcaatgtaac
     4021 ctcagcataa ggaggatcaa agggaattcc ttcatctaag ctgaatcttc ggcacatact
     4081 gccggcagta aaatagcgga gtacaaattc caactttggt acagtaagat ttcttacttt
     4141 catcagccca ttttctatat ccgctccaag gtaatgggta ggtatgccgt ttttctccat
     4201 gagctcaaaa aaatacttgg aaattataag gccgattttt cccttgcctt caacgattcc
     4261 accaacagta ttggagccgg ggtcaaaaac tccgttttct ccggttgcgc tgtctttaaa
     4321 aaagatatat accgaattat ccttctcatc ttttaaaact ttctttgttt ttccctcata
     4381 cagtgttttc attgctgttc tcctcttcaa agtaaaattt atctgtaatc gtcttgtctt
     4441 tgcattcttc tttcggcatc ttttttggca atatcttgac gtttgtcata agtcttttta
     4501 ccttttgcta ccgagagttc cattttaaca agaccatttt tcaaatataa acttaaaggt
     4561 ataagtgaat agccttttaa agttgttagt cctgcaagtt ttcttaattc ccttttgttt
     4621 aaaagcagtt ttctcggtct taaggggtct acattatata tgtttccctt ttcataagga
     4681 cttatgtgca tattataaac aaatgcttca ccatttttta tacttacata gctgtccttc
     4741 aaattgacct tcccctgtct tattgattta acttcagttc ctacaaggac taatcctgct
     4801 tcatatttat cttctataaa atattcatga aatgcctttt tattgttggc tacaatttta
     4861 tccgccattc taccacctac cgttaacgaa aatgattata gcacttaata attannnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgcat tttgcatagt gcattataac
     5041 ttcttccatg ggcttatcta ttaatatctc atgtttcatt ctttcttcat cggtggaggt
     5101 aagctgtgtt actgcaacta atatcggtct tgagccttcc ttgcgggtta aaccttcaat
     5161 agcagcctcc atcattgcaa tggttcctgc tgcatgaatg tttaccatat caacattgta
     5221 ttccgacaaa actttcattg cttttctaac ggtattggga atatcatgaa gcttaaggtc
     5281 caaaaatatt ttatgaccca tggatttaat gagttttaaa atatcaggcc cttctgcgta
     5341 aaataattcc atacccacct ttacataggg cttttcttca gtaaaatttg aaagaaaatt
     5401 tacaacttct tttctattgc tgaaatcaca agcaataatt acatccttgc ccatatcgtc
     5461 tccttattca tattaatttt cccatttttt gttttattaa tacttaatca ttgcatctgc
     5521 gacgggacat atatagataa ctatatcccc ttcttcttta agttttttat cactcaataa
     5581 tctgtcagca actttctcag tggaatttga ttttgtaata aatataataa tttctttttc
     5641 ttgctgcaac tctacattta taataggcaa cataacttta tgatgtatgt ctgtccctct
     5701 tccatgcata atggtagctc cgcttgcacc tgactgacgc gctaggtcta ctacattttc
     5761 agcaaagcct ctttctacat ttaccattat gcagtcgtag agcaatctgt tttcttcagg
     5821 tatagacgga gtaataacaa attcttcctc agtcggagta tattcttcaa ctttcttata
     5881 cctaaaaacg gttcccaaca gcatgattga aaatacggga gccattgcta ccatcgcaat
     5941 aacgccaaaa ccatccacta agacattagc tgtctctata gaagatgcag ctccttgagc
     6001 aaaggcaaga acaaaggttg cagtcatggg acctgaagca actcctcccg catcatatgc
     6061 aattcccaca aagacaggct ctgcaataaa tgatagcaaa at
//
LOCUS       DAOPUE010000106         6036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13033_length_6036_cov_9.578331, whole genome shotgun sequence.
ACCESSION   DAOPUE010000106 DAOPUE010000000
VERSION     DAOPUE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13033_length_6036_cov_9.578331"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..608
                     /locus_tag="PKH42_08300"
     CDS             126..608
                     /locus_tag="PKH42_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOA20473.1"
                     /translation="MNKNKLTALILIMLFVLSPVAFAFGTITASNPGYEERVVQLVNA
                     ERARYGLDALYMDSRISDVARLKSKDMADLNYFSHYSPTYGMASDMLLRFGINYSAWG
                     ENIASGQDTPEIVVSQWMNSPSHRENILSPYFTFIGVGYYVGADGTPFWTQMFTSDDK
                     "
     gene            888..1358
                     /locus_tag="PKH42_08305"
     CDS             888..1358
                     /locus_tag="PKH42_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20474.1"
                     /translation="MKYYSNPNKYRNSYINKFNMNNNSNNEGTGFLEITVSDAQTGMP
                     VANVDIEVFKMTIMGEFAERAVSKLVVRYSTFEDGTIPLIELPVIEWPLNRYFARLDT
                     FGYYDVSIVNIPIYDGVKTIYEVKLNRITSPTRIREYIRTPTRTEYYTPPIWFY"
     gene            complement(1453..2649)
                     /locus_tag="PKH42_08310"
     CDS             complement(1453..2649)
                     /locus_tag="PKH42_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015713794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA20475.1"
                     /translation="MKYDFTSIIDRRGMDSVAVDLRPGSGPEAPKEGFDVIPMWIADM
                     NFPTVPTIQEAIIERAKHPMFGYFLPKEEYFDSIIKWHEVRNGVTGLTKEHIGYENGV
                     LGCAMSAVNAFSAPGDCVLLHSPTYIGFTSSLENNGRKIIHSDLVRDEAGVWRMDYDD
                     MDAKLKKYNIHVAVFCSPHNPCGRVWERKEIEKAMEVYKRNDCVVISDEIWSDIILYG
                     NKHIPTQSVNEDAKNRTIALYAPSKTFNLAGLVGAYHIIYNKYLRDRVRAQGSKSHYN
                     ELNIFSMHALIGAYKKEGYEWVDELCQVLGENIDYASEHIINNYHGIKFFKPEGTYMM
                     YLDCEEWLKEHNMTLYDLYKKGTDVGVMWQDGRPFNHPYAIRLNLALPKSKVIEAFER
                     LDKYVF"
     gene            complement(2747..3613)
                     /gene="panB"
                     /locus_tag="PKH42_08315"
     CDS             complement(2747..3613)
                     /gene="panB"
                     /locus_tag="PKH42_08315"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOA20476.1"
                     /translation="MAKKKTILDLYKMKENGEKAVWITAYDCCFAAYAEKAGMDMILV
                     GDSMGMIVYGYQGTTPVTMDMCINHCQAVRRGAPNTYIIGDMPFGSYHESVEDAVRNA
                     VRFIKEADVDAIKLEGGVSVADKIKAIAQAGIVVFGHVGLTPQSSAALGGFKAQGRTT
                     ESARAVIEDAIAVYEAGAKFILLEGVPEEVCAFVHKILPIPVYGIGAGADTDGQVLVI
                     GDALGMFEAFTPKFTKKYADIAGIATQAFVDYVKDVKEGIFPAPEHKYKISGDMAEFE
                     KLFDEIEKDIKF"
     assembly_gap    3641..3740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3868..5712)
                     /locus_tag="PKH42_08320"
     CDS             complement(3868..5712)
                     /locus_tag="PKH42_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20477.1"
                     /translation="MANNKNKEKEYQPIKLLKNRIYPTYQLYAVINSKVSAEEAMVIA
                     VLETFSWLRKRFRELTIPDEIDYPEPDKYKSVRLDDFKSFRINEGYVVDVVFIKEKGV
                     FAFQLVEPDLGPEPGNENQGRKPAPGRVFATNIAYRIYNGKLECGFKTVCSEPENTKA
                     ACEVFRLAVIRSIVLNELLGLDQIIPIIRDMHQINSIDKVDRFIDYIRSNERQLPFVL
                     TVEYKNPVDFNTLVKTKPNFDINNLLNRKIEDLIATTKVEDKEEIFEDRMRELAGKKM
                     GYAQFGFLPYRYINNFNDRLGCNISGGDVCVFYPERYNRKNLFKRDSIKLNEKKFLKD
                     LETELQEFPKYKNINFGNVIFINEARIEELNKIIELSNSKEEIIKAIEDKIEIIEQKY
                     EDKIAVLNNEMHEKDEKIDTLKAEIKELNDKFDKFYLQVEKLKSDYNDQINKLNLKIE
                     WKDNKLKQPRKTEDIPEWVEKQFAGKLIFHNRAIGEIKKTPTGEIDMSLLCDAIEYLA
                     NEYRDELLGLISEVESNNICSDKYNRSFVVAPTGDKSIEMYTKEYKVKYKKGPAEKSR
                     EVLLDQHLKVGKGNKDNLRIYFFYDEEKDLIVVGSLPKHLSSVNKKKD"
     gene            complement(5746..6021)
                     /locus_tag="PKH42_08325"
     CDS             complement(5746..6021)
                     /locus_tag="PKH42_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20478.1"
                     /translation="MKRKTKKINNHYVVDEIINHDENGYSGEAIELLAKFENYYEDLV
                     SRQEAIIKEMDKLKAENKTNTVKFKQLFANKLNNSANLLILKQFGIH"
BASE COUNT         1808 a         1217 c          850 g         2061 t
ORIGIN      
        1 ataaggaaaa aaatttaaaa taagcaacaa atatacaacc tatcaaatat atgaaattat
       61 atattatgaa taaattgaaa caatattatt gaaacaaatt tttttgtttc agaacaggag
      121 gttgtatgaa caaaaataag ttaacagcat tgattttgat tatgctcttt gtattgtcac
      181 cggttgcatt tgctttcggt acaataacgg catcaaaccc cggttatgaa gaaagagtcg
      241 tccagttagt taatgccgaa agagcaagat acggattgga tgcactgtat atggacagta
      301 gaatttcaga cgtagccagg ctaaaatcaa aagatatggc tgatttaaat tattttagtc
      361 actattcacc aacctatggt atggctagcg acatgctgct tagatttgga atcaattatt
      421 ctgcctgggg cgaaaatata gcttcgggac aggatactcc tgaaattgta gtttctcaat
      481 ggatgaattc cccgtctcac agagaaaata tactttcacc ttattttact tttatcggtg
      541 taggatatta tgttggtgct gacggaactc cattctggac tcaaatgttt accagtgacg
      601 ataaataagc aggtgttcta atcgccaaaa aatacgttct ttttttaaag aacgtatttt
      661 ttaggaaaaa tcaccctaag ggtgattgaa aaccattttg ttaatatata taatttaata
      721 aataacacct aaatccaaaa aaatacattt tagtgctacc cttcctaaaa tgtatttttt
      781 cttattcaac ttgtcttaat tcctatctat atttaattca taacttaatt acaaccttta
      841 catataataa agcaagttgc atcggttatg atgaatggag gttaaaaatg aaatactatt
      901 caaatcctaa caaatataga aactcatata taaataaatt caatatgaat aataattcca
      961 ataatgaagg aacaggtttt ttagaaataa ccgtgtcaga tgctcaaacc ggcatgcctg
     1021 ttgccaatgt agatattgaa gtgtttaaaa tgactataat gggtgaattt gcagaaagag
     1081 ccgtatcaaa attagtagtt aggtattcta catttgagga tggtacaatt cctttaatag
     1141 agctgcctgt aattgaatgg ccgttaaacc ggtattttgc acgtttggat actttcggat
     1201 actatgatgt ttccatagtt aacatcccca tatatgatgg tgttaaaact atatacgaag
     1261 tcaaattgaa cagaattact tctcctactc gtatacgcga atatataagg actcccacaa
     1321 ggacagagta ctacactcct cctatttggt tctactaggt gatttcataa gaaacagcaa
     1381 tagatttaac aaataaggga cattcctcca ttaccaaagc tattccaagt agcagaggtg
     1441 tgtcccctta tgtcaaaaca catatttatc caagcgctca aaagcttcta ttacttttga
     1501 tttaggcaat gctaagttta aacgaatcgc atagggatga ttgaaagggc gtccatcctg
     1561 ccacataaca cctacatcgg tacctttttt gtacaaatcg taaagtgtca tattatgctc
     1621 ctttaaccac tcctcgcagt caagatacat catgtaggtt ccttcaggtt taaaaaattt
     1681 aattccatgg tagttattaa taatgtgctc acttgcataa tctatatttt caccaagcac
     1741 ctgacacaat tcatcaaccc actcataacc ttcctttttg taagctccaa tcaatgcatg
     1801 catagaaaaa atattgagct cattgtaatg agacttgctg ccttgggctc tgacacggtc
     1861 acgtagatac ttgttataaa taatatgata agctccgact aaaccggcaa gattaaacgt
     1921 cttgctggga gcataaagag caatggtgcg attcttagca tcttcattta cgctctgtgt
     1981 tggtatatgt ttgtttccgt agagaataat atcgctccaa atttcatcgg aaattacaac
     2041 acagtcattt cttttatata cttccattgc cttctcaatt tcttttctct cccatacgcg
     2101 cccgcatgga ttgtgcggag aacaaaaaac agctacgtga atattatatt tcttaagctt
     2161 tgcatccata tcatcataat ccatacgcca tactccggct tcatcccgta cgaggtcaga
     2221 atgtataatt tttcggccgt tgttttccaa agatgatgta aatcctatat aagtaggtga
     2281 atgaagaagc acgcagtccc ccggagcaga aaatgcattc actgcagaca ttgcacaacc
     2341 aagcacacca ttttcataac cgatatgttc tttggtcaat cctgttactc cgtttcgaac
     2401 ttcatgccat tttataattg aatcaaaata ctcttcttta ggcaaaaaat atccaaacat
     2461 cggatgcttt gcacgctcga taattgcctc ctgaatggta ggaaccgtag gaaaattcat
     2521 atctgcaatc cacattggaa ttacgtcaaa tccttccttt ggagcttccg gtcccgaacc
     2581 gggtcttaaa tcaacggcaa cggaatccat gccgcgtcta tcgataatac ttgtaaaatc
     2641 atatttcata tttaatattc ccctcattta tttagtataa cttgttctta cacatgacaa
     2701 aaatgcagcg tgctgatgca tcgtctgaca tccttcaaat tataaatcaa aatttaatat
     2761 ccttttcaat ttcatcaaac aacttttcaa actctgccat gtctccggat attttatatt
     2821 tatgctcagg tgccgggaaa attccttctt ttacatcttt gacataatcc acaaaggctt
     2881 gtgttgcaat gcctgctatg tcggcatact tctttgtgaa tttaggtgta aaggcttcaa
     2941 acattccaag agcatcgccg ataaccaata cttgaccgtc cgtatcagct cctgccccga
     3001 ttccataaac cggtataggc aatattttat gaacaaaagc acatacctct tcgggaacac
     3061 cttccaacaa tataaatttt gctcctgctt cataaacagc tatggcatct tcaattacag
     3121 cacgtgcact ttcagttgta cgcccctgag ccttaaaccc gcctaaagca gcagaacttt
     3181 gaggagttaa tccgacatgc ccaaatacaa ctattcctgc ttgtgctata gctttaattt
     3241 tatctgcaac agaaacgccg ccctctaatt ttatggcatc aacatcagct tcttttatga
     3301 aacgtactgc gttacgcaca gcatcctcaa cactttcatg atatgagcca aatggcatat
     3361 caccgattat atatgtattg ggagctcctc ttcttactgc ctgacaatga ttaatgcaca
     3421 tatccatagt aacgggagta gtgccttgat atccgtagac tatcattccc attgaatctc
     3481 ccaccaaaat catatccatg ccggcttttt ccgcatatgc tgcaaaacag cagtcatatg
     3541 cagtaatcca tactgccttt tcaccatttt ctttcatttt atataagtct aaaatagttt
     3601 ttttcttagc catatcggca cccctctttc atcatagttt nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn agtctaaaat agtttttttc ttagccatat cggcacccct
     3781 ctttcatcat agtttactaa atattatttt tttaattata taccatacaa tttcggttga
     3841 ccactttttc ttttaatttt ctgatttcta atctttcttt ttattaaccg agcttaaatg
     3901 ctttggcagg gagccaacga caattaagtc tttttcctcg tcataaaaga aatatattcg
     3961 aagattatcc ttgttgcctt tacctacctt cagatgttga tcaagcaaga cttctcttga
     4021 tttttcggct ggtccttttt tgtacttgac cttatattct ttggtataca tttcaatact
     4081 cttatcaccc gtgggagcta caacaaaact tctgttatat ttatcggaac aaatattatt
     4141 gctttccacc tcactgatta agcctaatag ttcatcgcga tattcatttg caagatactc
     4201 aatagcatca caaagcaaac tcatatctat ttcacctgta ggagtctttt taatctcacc
     4261 gattgccctg ttgtgaaaaa taagttttcc ggcaaattgt ttttcaaccc attcaggtat
     4321 atcttccgtc ttcctcggct gcttaagttt attatccttc cattcaattt ttaaattcag
     4381 cttgttaatc tggtcattgt aatcactctt aagtttctct acttgcagat aaaacttatc
     4441 aaacttgtca ttaagttctt ttatttctgc ttttaatgtg tcaattttct catccttttc
     4501 atgcatctca ttatttaaga cagctatttt atcttcatat ttctgctcaa taatttctat
     4561 cttatcttca atagctttaa taatttcttc ttttgaattg cttaattcaa taatcttgtt
     4621 aagctcttcg attcttgctt catttataaa aattacattg ccgaaattta tgtttttgta
     4681 ttttggaaat tcctgcagtt cagtctctaa atcttttaag aatttctttt catttaattt
     4741 aatactatca cgtttaaata aatttttacg attatacctt tcaggataaa atacacatac
     4801 atcaccgcct gaaatattgc acccgagtct gtcattaaaa ttattaatat atctataagg
     4861 caaaaatcca aactgcgcat accccatttt tttacctgcc aattctctca ttctatcttc
     4921 aaatatttct tctttatctt caaccttcgt agttgcaatt aaatcttcaa ttttgcggtt
     4981 caatagatta ttaatgtcaa aattcggttt ggtttttact aaagtattga aatcaaccgg
     5041 atttttatat tcgacagtca aaacgaaagg aagctgtctt tcattgcttc tgatataatc
     5101 aataaatcta tcaaccttat caatgctgtt gatttgatgc atatccctta taatgggaat
     5161 aatttggtcc agtcccaaca gctcattcaa aacaatagac ctaataacgg ctaatctaaa
     5221 cacctcgcaa gcagccttcg tattttcagg ctcagagcag acggtcttaa acccgcactc
     5281 caattttccg ttataaattc tataagctat attggtagca aatactctgc cgggtgccgg
     5341 ttttcgtcct tgattttcat ttccgggttc aggtcctaaa tcaggctcaa ccaactgaaa
     5401 tgcaaagaca cctttttcct tgataaaaac cacatccacc acataacctt cattaatgcg
     5461 gaaggattta aagtcatcta atctaacact tttatattta tcaggttcag ggtaatcaat
     5521 ttcatcgggt atagttaact ctctaaaacg ttttcttaac cacgaaaaag tttctaacac
     5581 agctataacc attgcttctt cagcagatac cttgctgtta attacagcat aaagctgata
     5641 tgtaggataa attctatttt ttagcagctt aatcggttga tattcttttt ctttattttt
     5701 attatttgcc atatccccca ctccttcaat agaaacactg atttattaat gaattccaaa
     5761 ttgtttcaat atcaacaagt tagcactgtt atttagctta tttgcaaaaa gctgtttaaa
     5821 ctttaccgta tttgttttgt tttcagcctt tagcttatcc atttctttaa ttattgcttc
     5881 ctgtcttgaa actaaatcct cataatagtt ttcaaactta gcaagcaatt caattgcttc
     5941 gcctgaatac ccgttttcat catgattaat aatttcatca actacataat gattatttat
     6001 tttcttagtc tttcttttca tattattaca tcctta
//
LOCUS       DAOPUE010000107         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13137_length_6007_cov_11.268985, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000107 DAOPUE010000000
VERSION     DAOPUE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13137_length_6007_cov_11.268985"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="PKH42_08330"
     CDS             complement(<1..2434)
                     /locus_tag="PKH42_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20479.1"
                     /translation="MIKRKYFYNMLIVATVSIFLFSLCNIVYGVEFNDDIQKITLNYD
                     YFDGIENDKILRSKGDGSWHLLKSDGSKIKLKGNYLDYAGIEKGEYYKLDEDYYRFVV
                     PIDNQLTRFAFVDKNGVECFVEGYSFADPTINNKYSLLMNVDSDTNYKFGLYDRYNDK
                     VVIPVVYDSLLYLNDDRIIARNGDTEGIIDINQKIILPFEYNLLEYLNDDFIIALGNN
                     FKYGLINIKNENLMPFEYHVISKASDTNNYCRIIKDNKIGLINGDNSESLIPIEYDSL
                     YYIDEMYIGKELIVAEKDDKTGIVDMDNKEIVPFIYDGIELIGNCFEVYKDGAAGLLD
                     SKGNEILPTEAIDIMEVKNDFITAKVYVDYNHKYAVYDMKGNEIIPPVYDYINYHASE
                     KYMIVNNDGIANLVDKAAKDEVLNKSYYSDIWYINDKYFAGGNNSYYSIVNFSGQELT
                     PSYYSTISVVNVEGEELLAALWQTPKSFDRRIDYFKQTRGPSAWAVEEVSKAIESNLV
                     PFEYQSAFTFNIKRNEFCTIMVEFLEEYYDISREEIIKDKNIDVTNQAFVDGINEDIA
                     ICLHLGIVKGRGNGIFDGESEITREEAAVMLTNLAKHLGLNTVADEVYLNDKKEVSEW
                     AIDAVNFVLENKFMQGVGNDMFSPKSNITREQTYIIMYRILHETEFYSLFNKASEAWG
                     WFYVGTMPLKVTPGLSFVEIETESGICYEVDYEGIETLEDLENYLKTIFSDDKVEGFL
                     KSGRYFDVGGKLCAYDMARGTNHSYGKIAEVNKNIISENQIEYTVSVEKLDYNYELEG
                     YEEFTFITERSGD"
     gene            complement(2730..4445)
                     /locus_tag="PKH42_08335"
     CDS             complement(2730..4445)
                     /locus_tag="PKH42_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOA20480.1"
                     /translation="MRLQISKSKNAASFYVTKSVYDKGKRTSKIVEKLGTYDELKSKL
                     GDKDPYEWAKEYVEELTRLEKEGKEPVVIAEYSPSKLIDSNIQCTFNGGYLFLQQIYY
                     ALGLHRICDGISNKYKFTFDLNSVLSRLSYGRILFPASKLATYEISQKFIEQPNFELQ
                     HVYRALEYIAKESDFIQSELYKNSLKISKRNTGILYYDCTNYFFEIEQEDGLKQYGFG
                     KDHKPNPIVQMGLFMDGDGIPLAFSITKGNTNEQVTLKPLEKQILSDFDLSKFIVCTD
                     AGLASTANRKFNNIEGRAFVTTQSIKKLKKHLKEWALDTNGWHLSDENKIYDISNLDE
                     EKYMDKIFFKERWIKEDRLEQKLIVTYSLKYKNYQRQIRNNQIERAKKIIRTNPKKLK
                     KCNANDYKRFVAKIHCTSEGEIAKKELYSIDTNLIANEEAFDGFYAVCTNLDDDASQI
                     AEINRRRWEIEECFRIMKSEFKARPVHLSRDDRIAAHFSTCFISMIIYRILEKRLEEK
                     YTCHEILSKLREMNFYELKGEGYIPTYTRNDLTDDLHEKFGFRTDYQIVSKKQIKNIF
                     KETKR"
     gene            4718..5248
                     /locus_tag="PKH42_08340"
     CDS             4718..5248
                     /locus_tag="PKH42_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOA20481.1"
                     /translation="MEQYSNKKFSLFEFNNLEANDENKYELIDEIVLMSPRPNYMHQQ
                     IMSRLNLEIGTYLKGKKCMVFSEVELEFDNNVIIPDLSIICGLNDFNIQRYKKAPELV
                     IEIISPGSQYTDTFTKLYKYELFGVKEYWIANPKTEIITVYNFENKTNKDYGKQEKLI
                     SSVFNDLEINLSEIFL"
     gene            complement(5472..>6007)
                     /locus_tag="PKH42_08345"
     CDS             complement(5472..>6007)
                     /locus_tag="PKH42_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20482.1"
                     /translation="VAGPSILEKNEEIYKVDSVPVGYPIITLEEISKESNQKGELSRD
                     FRQSMSPIVPKHHTYYEYENLGHSSKSIRIKYYEAINTYFADIIFNGITKELEKGMKW
                     QGMTLFEKIIITDDEMRKLWDMDNLALTEERDEIIIQKGNIVLHVDNYTGVMDFNDKE
                     TRELIISRFFSGSTVEN"
BASE COUNT         1953 a         1082 c          727 g         2245 t
ORIGIN      
        1 agtcaccgct tctttcagta ataaaggtga attcttcata tccttccaat tcataattat
       61 aatctaattt ttcaactgaa acagtatact cgatttgatt ttcacttata atattcttat
      121 ttacttccgc tattttacca taactatgat tagtacctct ggccatatca taggcacaca
      181 atttgccgcc cacatcaaag tatctgcctg attttagaaa accctcaact ttatcatcag
      241 aaaatattgt ttttaaatag ttctccaagt cttctaaagt ttcgatacct tcataatcca
      301 cttcgtaaca tattcctgat tcagtctcaa tctcaacaaa agacaatccc ggtgttacct
      361 tcagaggcat ggttccaaca taaaaccaac cccaggcctc acttgcttta ttaaataaag
      421 aataaaattc agtttcatgc agtattctat acataataat ataggtttgc tcccttgtta
      481 tatttgattt aggagagaac atatcattac cgacgccctg catgaatttg ttttcaagaa
      541 caaagttcac cgcatcaata gcccattctg atacctcttt tttgtcattt aaatagactt
      601 catctgcaac agtattaagt cctaaatgtt ttgccaaatt agtaagcatt acagctgctt
      661 cttctcttgt aatttcactt tctccgtcaa aaataccgtt acctcttcct ttaactatac
      721 ctaagtggag gcagatagct atatcttcat taattccatc aacaaaggcc tgattggtca
      781 catctatatt cttatcctta ataatttctt cccttgaaat gtcataatac tcttccaaaa
      841 attctaccat tatggtacaa aactcatttc tttttatatt aaaagtaaat gctgactggt
      901 attcaaatgg aactaaatta ctttctatgg ctttgctaac ctcttccaca gcccaagctg
      961 atggacccct tgtctgttta aaataatcaa tccttctgtc aaaactttta ggtgtttgcc
     1021 acaatgctgc taatagctcc tcgccctcaa cattaacaac agatatagtg gaataatatg
     1081 aaggagtaag ctcctgaccg gaaaaattaa caatactata atagctgttg tttccgccgg
     1141 caaagtattt atcatttata taccaaatat ccgaataata gcttttgttt aatacctcat
     1201 cttttgcagc cttatctacc aaatttgcaa taccgtcatt atttacaatc atatattttt
     1261 cagatgcatg ataatttata taatcataaa cgggtggtat tatttcattg cctttcatat
     1321 cgtaaacagc atatttgtga ttgtaatcaa cataaacctt agctgtaata aagtcattct
     1381 taacttccat tatatctata gcttctgtag gcaatatttc atttcccttg ctatctaata
     1441 agcctgctgc tccatctttg taaacctcaa aacaattacc tattaactca atcccatcat
     1501 aaataaatgg aacaatttct ttattgtcca tatcaactat tccggtttta tcatcttttt
     1561 cggctacaat caattctttt cctatgtaca tctcatcaat atagtataaa ctgtcatatt
     1621 ctatgggtat taatgattca gaattatccc cgtttattaa accaatctta ttatccttta
     1681 ttatcctgca ataattgttt gtatccgatg ccttgcttat cacatgatat tcaaaaggca
     1741 ttaaattctc attttttata ttaattaagc catatttaaa attatttcct aacgctatta
     1801 taaagtcgtc atttaaatat tctaaaagat tatattcgaa tggaagtata attttttgat
     1861 taatgtctat tatcccttcc gtgtctccat ttcttgcaat tatcctgtca tcattcaaat
     1921 ataataaact atcatatact acaggtataa ctactttatc attgtatctg tcatacaacc
     1981 cgaatttata atttgtatca ctgtcaacat tcattaacaa cgagtattta ttattaattg
     2041 ttgggtcagc aaatgaatac ccctctacaa aacactccac accgttttta tcaacaaaag
     2101 caaatcttgt taattggttg tcgatgggaa ctacaaacct ataataatct tcatctaatt
     2161 tatagtattc acctttttca attccggcat aatccaaata attacccttt aatttaattt
     2221 tgctgccatc agattttaat aagtgccagc taccgtcacc tttgctccgt agtattttgt
     2281 cattttcaat tccatcaaaa taatcgtaat taagtgtaat tttttgtatg tcatcattaa
     2341 attccactcc atatacaata ttacaaagcg aaaataaaaa aatactaacg gttgcgacaa
     2401 ttaacatatt gtaaaaatat tttcttttta tcactacctt ctcctccaaa atttttatta
     2461 aaatattagt tgccttttgc gtcaatcatt ttgtaaaatt acctttcctt taaattctac
     2521 ctcaaaagtt ttctgtattc aactaattct tcattatttt caaaagtaag aatcccgctt
     2581 caaaagatat attattattt tattgttatt taataaatca tttgatataa tcctgtcttt
     2641 gacagttgag agaaattaaa accctgaaac ccattgtatt tacaatgaat ttcagggttt
     2701 ctatttgttt gtaaaagtga ataatgtttt tatcttttgg tttctttaaa aatattttta
     2761 atttgttttt tagatactat ttgataatct gttctaaagc cgaatttttc atgtaaatcg
     2821 tccgttaaat cattacgagt atatgttgga atataacctt caccttttaa ttcatagaaa
     2881 ttcatttctc ttaatttaga aagaatttca tggcatgtat atttttcttc taaccttttt
     2941 tcaagtatgc gatatataat cattgaaata aaacaggtag aaaaatgtgc cgctattctg
     3001 tcatcacgac ttaaatgaac aggtcttgct ttaaattcgc ttttcattat tctaaagcat
     3061 tcttcaattt cccatcgtct tctgttgatt tcagcaattt gagaagcgtc atcgtcaaga
     3121 ttagtgcata cagcataaaa accatcaaat gcttcttcat ttgcaattaa atttgtatca
     3181 atgctatata attccttctt agcaatttca ccttctgaag tgcaatgaat tttcgcaaca
     3241 aatcttttgt agtcattagc attgcacttt ttaagttttt taggatttgt gcgaattatt
     3301 tttttagcac gttcaatctg attattacgg atttgacgct gataattctt gtattttaaa
     3361 gaatatgtaa ctattaactt ttgttctagt ctgtcttctt taatccaacg ttctttgaag
     3421 aaaattttat ccatgtattt ttcttcatca agatttgaaa tatcataaat tttattctca
     3481 tctgataaat gccagccatt tgtatccaaa gcccattctt taagatgctt ttttaatttc
     3541 ttgatagatt gggttgtaac aaaagctctt ccttcaatat tgttgaattt cctattagcg
     3601 gtagaagcaa gcccagcatc agtacacaca ataaatttag agagatcaaa atctgaaaga
     3661 atttgttttt ctaaaggttt caaagtaacc tgttcattgg tattgccttt ggtgatgcta
     3721 aaagctaagg gaataccatc accatccata aaaagtccca tctgtacaat aggatttggt
     3781 ttatggtctt ttccaaatcc atactgtttt aaaccatctt cttgttcaat ttcaaaaaag
     3841 tagtttgtgc aatcataata gaggatacca gtatttctct tagaaatctt taaactgttt
     3901 ttatataact cagactgtat aaaatccgat tcttttgcaa tgtattcaag agcacgataa
     3961 acatgttgca gctcaaagtt tggctgttca ataaattttt gcgatatctc ataggttgca
     4021 agcttagaag ctggaaacaa aattcttcca taagaaagtc ttgaaagtac agaattaagg
     4081 tcaaatgtaa atttatattt atttgaaatt ccatcacata ttctatgtaa tccaagagca
     4141 taatatattt gttgaagaaa tagataacct ccattaaaag tgcattgaat attactatca
     4201 attagtttgg atggtgaata ttctgcaatt acaacaggtt ccttgccttc tttttcaaga
     4261 cgagttaatt cttcaacata ttcttttgcc cattcatatg gatctttatc tccaagctta
     4321 ctttttaatt catcataggt gcctaatttt tcaacaattt ttgaagttcg tttccctttg
     4381 tcataaacgg atttagttac ataaaaagat gcagcatttt ttgatttaga aatttgaagt
     4441 ctcatagaaa ttaacacctc cagttcctta tattataaca cattccgaca cattgtgcaa
     4501 taattatttt taaaaattga caaaaaaata aagctttttc aatagcttta gcgatttctt
     4561 tattcatcaa ctgtcaaaga cccgtattca actaattctt cattattttc aaaagtaaga
     4621 atcccgcttc aaaagatata ttattatttt attgttattt aataaatcat ttgatataat
     4681 tataaaaaac agcgtatata aattattgga ggtaacaatg gagcaatatt caaataaaaa
     4741 attttcactg tttgaattta ataatttaga agctaacgat gaaaataaat atgaattaat
     4801 tgatgaaatt gttttaatgt caccacgacc aaattatatg caccagcaaa ttatgagcag
     4861 gttaaacctt gaaataggaa cctatctaaa gggaaaaaaa tgtatggtgt tttctgaagt
     4921 tgaattagaa tttgataata atgttataat tcctgactta tcaattatat gtggattgaa
     4981 tgattttaat atccaaagat ataaaaaagc ccctgaactt gtaattgaaa ttataagtcc
     5041 tggcagtcaa tatacggaca cattcacaaa gctttataaa tatgaactat ttggagttaa
     5101 agaatattgg atagctaatc caaaaactga aattatcaca gtatataatt ttgaaaacaa
     5161 aactaataaa gattacggta aacaggaaaa attaatatcc tctgttttca atgatttaga
     5221 aattaactta tccgaaattt tcttataggt tcccaaaaat gtttatgacg gtaaaaggtt
     5281 ttccttgaaa tgtgcaaatt tttgactaat acagtatttt atgaaaataa atgctgttcc
     5341 tccctgcatt aatctaattt ttgcacaccc gtcaaaacct tgcgaatata ttcaatgata
     5401 atacttaagc cacacgatat aattgcaaaa ataagattta aagcaacaat gtgtttcatt
     5461 cttatgtgaa attagttttc cactgtagag cccgagaaaa atctgctgat tatcagctct
     5521 cttgtttctt tgtcattaaa atccataact cctgtataat tatccacatg cagcactatg
     5581 tttccttttt gaataataat ttcatccctc tcctctgtca gtgccaaatt gtccatatcc
     5641 cataacttcc tcatctcgtc atcagtaata attattttct caaataaggt catcccttgc
     5701 cacttcattc ctttttctaa ttccttcgtt attccgttaa aaataatatc ggcgaaatat
     5761 gtatttattg cctcataata tttaattctt atgcttttcg aactatgacc caaattctca
     5821 tattcataat aagtgtgatg cttaggtacg atggggctca tgctttgtct aaaatctcgg
     5881 cttaactcac ctttctgatt tgattccttt gatatttctt ctaatgtaat tataggataa
     5941 cccaccggta ctgaatctac tttataaatt tcctcgttct tttccaaaat agaaggtcct
     6001 gctactt
//
LOCUS       DAOPUE010000108         5970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13248_length_5970_cov_9.682671, whole genome shotgun sequence.
ACCESSION   DAOPUE010000108 DAOPUE010000000
VERSION     DAOPUE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13248_length_5970_cov_9.682671"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKH42_08350"
     CDS             complement(<1..606)
                     /locus_tag="PKH42_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20483.1"
                     /translation="MERDNNIVGILRDKDEADLDGVLKTTFGGYTKQSVQEYLSLIHK
                     QQQAAKETFSKNLQTLFDEKEELRIKNETLQARLNKLSSEYDNLSCSLKNIMLDDSEY
                     SAQNVLELKNKIVSLEEQVKVTNRENYSLERKIEQLNNEIDELKTKLEISNKEYESQK
                     EMLKSERLELKKQRDIVADLSNKLEEEKNEVKYLKGTISDGK"
     gene            complement(832..1158)
                     /locus_tag="PKH42_08355"
     CDS             complement(832..1158)
                     /locus_tag="PKH42_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOA20484.1"
                     /translation="MKLYVNDKLLSGNIKSADSFKTKLIGLLNKKSLDKNEGLLLTNC
                     SGIHCFFMKFTIDAVYLSKDMKVIYKERVKPWRTGKLVRNCTHILELPEGMAKDIEIG
                     DYISLL"
     gene            complement(1158..2090)
                     /locus_tag="PKH42_08360"
     CDS             complement(1158..2090)
                     /locus_tag="PKH42_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20485.1"
                     /translation="MTLYISVVFAIFIFSLIIIILNKYGLNRDKINNRLSKLIEIDND
                     VIVDEELNKSLAERFISPIMDSLVKSLGKILPDTGLNNQKSENLRKLLRQAGYTIKPK
                     EYSAIKIIIIWGNALLYFILGLVLHVSYLYLFLLPLFGAYTAFTVMRFNLMFRITKRK
                     DEMERQLPDVLDMLSVNVEAGLGFEQALLHIIEHFEGPLIDEFHITYREMSMGRSRRD
                     ALMLLGERCDIDDLKSFTGSIIQANKLGISLKNVLHSQAASIRENRRSKIEEKAHKMS
                     IKILLPMVMFIFPVIFIILMGPAVVSIVEMFGGM"
     gene            complement(2098..3042)
                     /locus_tag="PKH42_08365"
     CDS             complement(2098..3042)
                     /locus_tag="PKH42_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20486.1"
                     /translation="MKILISLMVALFIYLCFNILISSYGPGQARSRIKRYFTEYSVDD
                     VQEQFIREKSEEESKRKKNRFKIASKEFSNLIASSGIRLKANEFLYFWAGLTLIPMMV
                     VLLFSGNIITGAALSIIGISIPPFIVSSARKKREELFTKQLGDATIIMGNSIKGGFTF
                     LQSMENIAEEMQPPISTEFKKVLREIHYGVKQEDALNHMVERTKNKDLELLISAVMTS
                     AQVGSNLSDILDTISNTIRDRIRIKNEIKTLTAQGRMSGLVIGLLPIVLILAIMVLTP
                     SYFEGFFDSTIGKILIAVSVLLEITGFAIINKIINIKV"
     gene            complement(3051..4385)
                     /locus_tag="PKH42_08370"
     CDS             complement(3051..4385)
                     /locus_tag="PKH42_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HOA20487.1"
                     /translation="MSLLDRLEKQKFSVSGERSAEITLYDEYSDLKERVHKDFIETVN
                     QQDISLFGVKGEQEEELLKIMDSLIESKAPKISRTERSRLLKEIYNDVMGLGPLEALL
                     NDDDITEIMVNGPSQVYVERKGKLELSDVVFKDNTHVMNIINRIVSTVGRRIDESSPM
                     VDARLKDGSRFNAVIPPLSLVGPSMTIRKFSKKPFTASDLIKFGSLSPKMVAFLEACV
                     KGRMNIIVSGGTGSGKTTLLNVLSSSIPNNERIITIEDAAEIQLNQEHVVTLESRPPN
                     LEGSGQITIRDLVRNSLRMRPDRIIVGEVRAGEAIDMLQAMNTGHDGSLTTAHANSPR
                     ELMSRLETMVLMSGMDLPVRAIREQIHSAIDLVVHQARMKDGSRKVVNITEVVGMEGD
                     TITLQDIFVYKVEGVDSNGRMKGYFTSTGIRPNFLDKLSSSGIIVRDDWFVN"
     gene            complement(4406..5617)
                     /locus_tag="PKH42_08375"
     CDS             complement(4406..5617)
                     /locus_tag="PKH42_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOA20488.1"
                     /translation="MKIKVLLISKMERNIEALRSKIEDEEIIILGDSVGGAQALDKIE
                     NTSPDIIIMTLGAGDTDVLNLAERIILHRPRTFVILVADTLNVEIMQEAVKVGCHNLI
                     EFPATSKEFAEYIKSVYHNESIRIKSLSNKDTLVWASRVITVFGAKGGLGKSTIAANL
                     AVKLSEKRKKVALVDLDLQFGDINIFLDIEPKDTIVELVQDVVTPNIDSVRSYMSVHS
                     SGVHVLCAPRSPEYAELVSAEKVQSLLSLLRTYYDYVIVDTSPSFTDVTITAIESASI
                     LFFVTGLDISILRNSKLSVSLLESLQQTDKIRLIVNRAVDMTSITINDVQKIIGYPIW
                     AKIPSDYKVAVNALNRGVPFVTGAPNSKLSASITELTDILLKGSTDYDLMGKHKNNNN
                     NIKKLRLFKKK"
     gene            complement(5629..>5970)
                     /locus_tag="PKH42_08380"
     CDS             complement(5629..>5970)
                     /locus_tag="PKH42_08380"
                     /inference="COORDINATES: protein motif:HMM:NF028286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HOA20489.1"
                     /translation="GMIKPHNTVDIIAQFQKTEKQPDGEEKTIDYTTMIAENIKVLAV
                     DDVISQEGKTKNPETGTAYTSVTLQVTVYQAMELNMSEYKGQLRLILRSPLDEDMVNP
                     PTIKFDDIIIN"
BASE COUNT         1656 a         1263 c          958 g         2093 t
ORIGIN      
        1 tttaccgtcg gaaattgttc cttttaaata ttttacttcg tttttttctt cttctaattt
       61 atttgataaa tctgccacga tatcgcgttg tttttttagt tcaagtcttt cggatttgag
      121 catttctttc tgagattcat attccttatt tgaaatttcg agcttcgtct tcagctcgtc
      181 tatttcattg tttaactgct caatttttct ttccagggaa tagttttcgc ggtttgttac
      241 cttgacttgt tcttccaagg atacaatttt gttttttaat tccaatacat tttgagctga
      301 gtattcggaa tcatcaagca tgatattctt taatgagcag gatagattgt catattcgga
      361 tgataatttg ttcagacgag cttggagtgt ttcatttttt attcttaact cttccttttc
      421 atcaaataag gtttgaaggt tttttgaaaa agtttccttt gctgcttgct gctgcttgtg
      481 gattaatgat aagtattcct gcacagattg cttggtatag cccccaaagg ttgtttttaa
      541 aacaccgtct aaatctgctt catctttatc gcgcaaaatt ccaactatat tattgtctct
      601 ttccataata ttacctctat cgtttatttt atttttaatg tatttatatt ctttgtgctg
      661 actctttgaa ttatcacggg ttactacttg aattatcacg ggttactaca caaggatgat
      721 tgtcatcctg agcggaagcg aaggatctca ggttttataa gatgagattc ttcgagcaaa
      781 gctctcagaa tgacactcag attattcata gcaatgacaa tcacgggttt ttcacagcaa
      841 tgaaatataa tctccaatct caatatcttt tgccatacct tccggaagct ccaaaatatg
      901 agtgcagttt ctgacaagct tcccggtcct ccacggctta accctttctt tataaattac
      961 tttcatatct ttggataagt agactgcatc gattgtgaat ttcatgaaaa aacaatgtat
     1021 tcccgaacag tttgtcaaga ggagcccttc atttttgtct aagctttttt tgtttaacag
     1081 tccaatcagc ttggttttaa agctgtcagc cgatttaata ttgccggaca gcaatttatc
     1141 attaacatat aatttcatta catcccacca aacatttcaa ctatggacac aacggcaggt
     1201 cccattaata ttataaatat taccggaaaa ataaacataa ccatgggcaa aagtatctta
     1261 attgacattt tatgtgcctt ttcttctatt ttgctgcgcc tgttttccct tatggatgct
     1321 gcctgtgagt ggagtacgtt tttaagagat atacctaatt tgttggcttg aattatggag
     1381 ccggtaaatg attttaaatc atcaatatca cagcgctcgc ctaagagcat aagcgcatct
     1441 cttctgcttc tgcccatgga catttctctg taagtaatat gaaattcatc aatcaagggt
     1501 ccctcaaaat gctcgataat atgcagcagg gcttgttcaa accctaatcc tgcctctaca
     1561 ttaacactga gcatatccaa aacatccgga agctgtctct ccatttcatc ttttcttttt
     1621 gttatgcgaa acattaagtt aaaccgcatt acggtaaaag ctgtataggc accaaacagc
     1681 ggcaacagaa ataaatataa ataactgaca tgtaatacta agccgagtat aaaatataaa
     1741 agtgcatttc cccatataat aattattttt atagcggaat attccttcgg ttttatggtg
     1801 tatcccgcct gtctgagcaa ttttctcaaa ttttccgatt tttgattgtt taagcccgta
     1861 tcaggcaaaa ttttacctaa ggacttaacg agcgagtcca taatagggct gataaacctt
     1921 tctgccaatg atttattcag ttcttcgtcc acaataacat cattatcgat ttcaattaat
     1981 ttagacagtc tgttattaat tttatcgcgg ttcaaaccat atttgtttaa tataataata
     2041 attaaactga atatgaaaat tgcaaaaaca accgaaatat aaagagtcat atatacctca
     2101 cacttttata tttattattt tattaataat tgcaaacccc gtaatttcca ataaaacaga
     2161 tacggcaatt aaaatttttc caatggttga atcaaaaaat ccttcaaaat aactaggagt
     2221 caaaaccatt atggctaaaa taagcactat cgggagcagt cctataacca gtcccgacat
     2281 tcgtccttgg gctgtcaatg tcttgatttc attttttatc ctgatgcggt ctcttattgt
     2341 atttgaaatt gtatcaagaa tatcggataa attgcttccg acttgtgccg aagtcattac
     2401 tgcggatatc aaaagctcca aatctttatt ttttgtcctt tcaaccatgt gattgagtgc
     2461 gtcttcctgc ttgacgccat aatgtatttc tcttagcact tttttaaatt cagtggatat
     2521 gggcggctgc atttcttctg caatattttc catggactgc aaaaatgtaa aaccgccttt
     2581 aatgctgttt cccattatta tggttgcatc ccccaattgc ttggtaaaaa gttcctcgcg
     2641 cttttttctc gcactgctta ctatgaaggg cggtattgaa atgccaataa tggacagggc
     2701 tgctcccgta attatgtttc cgctgaaaag taaaaccacc atcatcggta ttaatgtcaa
     2761 gcctgcccaa aaatataaga attcattggc cttcagcctt atgcccgagg aagcaataag
     2821 gtttgaaaat tccttgcttg ctattttaaa tctattcttt tttcttttgc tttcttcttc
     2881 acttttttct ctgatgaatt gctcctgaac atcatcaacg ctatattcgg taaaatacct
     2941 ttttatcctg ctcctggctt gtcccggccc atatgaggat atcaaaatat tgaaacacaa
     3001 ataaataaac aaagcaacca taagagatat tagaattttc atatttcacc tcaatttaca
     3061 aaccagtcat ctctgactat tatgccgctt gatgacagtt tatcgagaaa attcggccgt
     3121 atacctgttg acgtaaaata ccctttcatt cttccattgc tgtcaacacc ctcaaccttg
     3181 tatacaaata tatcctgcaa ggtaattgta tccccctcca tgcctacaac ctcggtgata
     3241 tttactacct ttcttgagcc gtctttcatt cttgcctgat gaaccaccaa atcgattgcg
     3301 gaatgtattt gctcccttat tgccctgacg ggcaagtcca ttccggacat taataccatt
     3361 gtttccaaac gggacataag ctctctcgga gaattggcat gggctgttgt cagtgagccg
     3421 tcatgacctg tattcatcgc ctgcagcatg tctattgctt caccggcacg tacctctccg
     3481 actattatcc tgtccggacg catacgcaaa gagtttctta ccaggtccct gatagtgatt
     3541 tgaccggagc cctctaaatt cggaggtctg ctttccagtg tcaccacatg ttcctgatta
     3601 agctgaattt ccgcggcatc ttcaatggtt atgatacgct cattattggg aatactgctc
     3661 gacaatacat tcaacaaggt tgttttaccg cttccggtac ctcccgaaac tataatgttc
     3721 attcttccct taacgcaagc ttccaagaat gcaaccattt taggagataa ggagccgaat
     3781 tttatcaaat cgcttgcagt aaagggcttt tttgaaaact ttctgatagt cattgaaggt
     3841 cctaccaatg aaagaggcgg gataacggca ttaaaacggg agccgtcttt taatctggca
     3901 tcaaccatgg ggcttgactc atcaatacgt cttcccacag tggatacaat acgatttata
     3961 atgttcatta catgtgtatt atctttaaat acaacatccg ataattccag tttccctttt
     4021 ctttccacat atacttgcga aggaccgttt accataattt ccgttatgtc atcgtcgttc
     4081 aataatgctt caagaggtcc taaccccatt acatcattgt aaatttcttt taatagtctg
     4141 gagcgctctg ttcttgaaat tttaggagct ttggactcaa ttaatgaatc cattattttg
     4201 agcaattctt cttcttgctc tccctttact ccaaataaac ttatatcctg ttgatttact
     4261 gtttcaataa aatctttgtg cactctttcc tttaaatcgg aatattcatc atacagagtt
     4321 atttctgccg acctttctcc cgaaacggag aatttctgtt tttcgagacg atccaataaa
     4381 ctcatttcga ttcctgcctt ttatgttatt ttttcttaaa cagtctaagc ttctttatat
     4441 tgttattgtt gtttttatgt tttcccataa ggtcgtaatc ggttgagcct ttaagcaata
     4501 tatcggtcaa ctcggtaatc gatgcgctca gcttgctgtt cggggcgcct gttacaaacg
     4561 gtacacctct gtttaatgca tttaccgcaa ccttgtaatc actgggtatt tttgcccata
     4621 tgggataacc tataattttt tgaacatcat ttattgtaat ggaagtcata tcaactgctc
     4681 tgtttacaat taacctgatt ttatcagttt gctgcaatga ttccagcagc gaaacactga
     4741 gctttgaatt ccttagaatt gaaatatcaa gaccggtaac aaaaaataat atggaagcag
     4801 actcaattgc ggtaattgtt acatccgtga atgacggtga tgtatctaca attacataat
     4861 catagtaggt tcttaacaga ctcaacaggc tttgcacctt ttctgcagaa accaattctg
     4921 catattcagg gcttctaggt gcgcagagta catgaacacc gctcgaatga accgacatat
     4981 agctcctaac ggagtcaata ttcggtgtaa ccacatcctg gactaattcc acaatagtat
     5041 cttttggctc tatatccaag aaaatattta tatcaccaaa ttgcaaatct aaatcaacca
     5101 gtgcaacctt ttttcttttt tccgaaagtt tcaccgctaa atttgctgca atcgtacttt
     5161 tacccaaccc gccttttgct ccaaatacag ttataactct tgatgcccat accaacgtat
     5221 ccttgttgct taatgattta atacgaatag actcgttatg atatactgac tttatatact
     5281 ctgcaaattc ttttgatgtt gcaggaaatt caattaagtt atggcaaccg accttaacgg
     5341 cttcctgcat aatttcaacg tttaatgtat cagcaaccag aataacaaat gttcttggtc
     5401 tgtggagtat aattctttcg gccaaattca gcacgtctgt gtctcctgca cccaaagtca
     5461 taataattat gtcgggagat gtgttttcga ttttgtccaa tgcctgtgct ccccccactg
     5521 aatcacccaa tattataatt tcttcatcct caatcttgga cctgagtgcc tctatatttc
     5581 tttccatttt actgataagc aagactttga ttttcataat tgcccccact aattaattat
     5641 aatatcgtcg aattttattg ttggaggatt aaccatatct tcatccaagg gagaccttaa
     5701 tattaatcta agctgtccct tgtattcgga catattcagc tccatagcct gatataccgt
     5761 gacctgcaat gttacagagg tataagcagt gcctgtttca ggattttttg tcttgccttc
     5821 ttgagatatt acatcgtcaa cagctaatac cttgatgttt tctgctatca tagttgtata
     5881 atctattgtt ttttcctctc cgtcaggctg cttttccgtt ttttgaaact gggcaataat
     5941 atctaccgta ttatgaggct taatcattcc
//
LOCUS       DAOPUE010000109         5956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13281_length_5956_cov_17.577868, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000109 DAOPUE010000000
VERSION     DAOPUE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13281_length_5956_cov_17.577868"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKH42_08385"
     CDS             complement(<1..844)
                     /locus_tag="PKH42_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20490.1"
                     /translation="MLVTKWKIFKEKYITKLSAFVLLIAFLMIGVSSALNIYLKVKNY
                     ESIAVKHYLESDTISNDLRSAAKRLEFIMRVYKNEDYILSGGTVDKIDIKDNWQLTNL
                     YNNYIAENNYEDNSETKELFFIDKHQEIQEIKESIINSDLANYEKIISELNLPQGYIY
                     YATDGENETTNTDNPDKDFYSLRNAYIFLDENGIELRPENGGIVSDSLTDIFESGTFN
                     KNMHIYAAIEDDALLNRVNRWNSDRQILIRNTAVIVLCSLLALACFSYLISSAGIAAG
                     NNESS"
     gene            complement(816..1508)
                     /locus_tag="PKH42_08390"
     CDS             complement(816..1508)
                     /locus_tag="PKH42_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA20491.1"
                     /translation="MNILVCDDDREIVDAIEAYLKSEEYNIFKCYSGKQAIETAEKNE
                     VHLIIMDIMMPEMDGITATTKIREIKNIPVIMLSAKSEESDIVLGLNMGADDYITKPF
                     NPLELIARVKSNLRRYTALGGALDMEKIDVYATGSLVIDDGRKEVTVDGELVKLTPVE
                     YKILLLLTKNKGSVYSIDEIYENVWNEPSFNPENTVAVHIRRIREKIEIDPKNPRYLK
                     VVWGVGYKVEDI"
     assembly_gap    1515..1614
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1982..2977)
                     /locus_tag="PKH42_08395"
     CDS             complement(1982..2977)
                     /locus_tag="PKH42_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOA20492.1"
                     /translation="MSYLLKPFDNGKIKLKNRLVMPPMATAKSDVDGSVSRDVLDYYE
                     EKSKGGYISLVIIEHSYISRDGKASLNQLSVEDDSMIEGLKKLAETIHKNGSKAVMQI
                     NHAGSNARMEVNQKPVGPSAILNPRKLTDLVPTELTKDNIKEIINNFKNAAIRVKKAG
                     FYGVEIHSAHSYLLDQFLSPLSNKRTDEYGGDIHGRIKIHLEIIEAVRKAVGDDYPLL
                     LRLGATDDREDGLTMDDAVQAAACFEKAGVDMLDISGGMCGYIPSNSNEQGYFSLQSE
                     IIKKEVGIPVILTGGITQPQSAENLLAESKADFIGVGRAVLKDSSWAEKALASLQ"
     gene            complement(3018..4607)
                     /locus_tag="PKH42_08400"
     CDS             complement(3018..4607)
                     /locus_tag="PKH42_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOA20493.1"
                     /translation="MREIIFGLIFALTFTPVNGEDTAASKIVKSLDLPGIVDILEPEN
                     SGLPEIDKSTELTIYSPQYNKYRNNAYGYEIIIPGNLKLNEDIVSVKSRFESDNLVVE
                     IFYDNFYNTVNSHSTYRYYGNRGIFRNPEFAIIDEYDCSYAGLSGHVTLFKRRKIKSD
                     QLDRNYYGTVTFARDTYESITVFMKSSSPLNIDEILPEFKFIEKTEILKKDAVFNPEQ
                     KNFDELTQEFYDQYFINNEKVDFGIFEPTFPKYPYRLQQIEKMLDYNFPVTLLYNNFS
                     LPFKTDSMNKAKESGKVVEYGLYTTDIINDKEKDITLDILEGKYDRYLDELAQKFNEY
                     DYPVLFRPNNEMNGEWVLYCSHKVGKDTDLFIDCWRYIYDKFEDHGVDNLIWVWNPNE
                     KSFPDFSYNNYLCYYPGDKYVDVVGLTSYNTGSYYKGEIWRSFPEAYDHFYYDYIERF
                     THPMMITEFSSSALGGDKAKWFEDMLNIIYKYDRIKIAILWNGRDYDKTKEEITVSRD
                     YTIDSEDTVINAVRNGLNKFK"
     gene            4726..5148
                     /locus_tag="PKH42_08405"
     CDS             4726..5148
                     /locus_tag="PKH42_08405"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOA20494.1"
                     /translation="MVTADTFKRGFKNSIYTLIELSKILIPVYIGVELLSVSGLLNIL
                     AEIFKPLMSILGLPGEASLVIILGYFLGTYAGLGVIAAIELNTVQITTLAIMISISHS
                     LISESAVVKKLGVSAAASVALRVSSSILMGFLYYRIFG"
     gene            5153..5608
                     /locus_tag="PKH42_08410"
     CDS             5153..5608
                     /locus_tag="PKH42_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOA20495.1"
                     /translation="MSGILDIVFNLLGFLWNIARVLVPIMIVIEIFKDTHLIDRISDY
                     FNPVSKFFTISKTAGISLLFGMFFGIIIGAGAMLQSVKDYNVDKRSVFLISMFISPCH
                     AIFEDAFIMNGAGANIVPLLASRFISAIMATFILSRVIKKEPLDAISNN"
BASE COUNT         1651 a         1116 c          956 g         2133 t
ORIGIN      
        1 cgctgctttc attgtttcct gccgctattc ctgctgatga tataagatat gaaaaacatg
       61 ccagagccaa aagagaacat aggactatca cagctgtgtt cctgattagt atctgcctgt
      121 cagaattcca tctgtttact ctgtttaaaa gtgcatcatc ttctatggcg gcataaatat
      181 gcatattttt attaaaggtt cctgattcga atatatcggt cagggaatct gaaactatgc
      241 ctccgttttc aggtcttaat tctatcccat tttcgtctaa aaaaatataa gcgttgcgga
      301 gtgaataaaa atctttgtca ggattatcgg tatttgtcgt ctcattttcc ccgtctgtag
      361 catagtaaat gtaaccttgg ggcaaattaa gctcactgat tatcttttcg tagtttgcca
      421 ggtctgaatt aattattgat tcttttattt cttggatttc ctggtgttta tcaataaaaa
      481 ataattcttt tgtttccgaa ttgtcctcat aattattttc tgctatatag ttattgtata
      541 aatttgtaag ctgccagtta tcctttatgt ctatcttatc aacggttcct ccgcttagta
      601 tatagtcttc gtttttatat acccgcatta taaattcaag acgctttgcc gcactcctaa
      661 ggtcattgct tatagtgtcg ctttccaaat agtgtttcac agcaatactc tcataatttt
      721 taaccttcaa atatatattc aaagctgaag atactcctat cataaggaat gctataagca
      781 gaacaaatgc tgataatttt gtaatatact tttctttaaa tatcttccac tttgtaacca
      841 acaccccaca ccaccttaag gtaccttgga tttttaggat ctatttcaat cttttctcta
      901 atccgcctaa tatggacagc cactgtattt tccggattga aggaaggttc gttccataca
      961 ttttcatata tttcgtctat agaatagact gatcccttgt ttttggttaa caagagcaat
     1021 attttatatt caacaggtgt aagctttacc aattctccgt ctacagttac ttcttttctt
     1081 ccatcgtcaa tcaccaggct tcctgttgca tacacgtcga ttttttccat atccaaagcc
     1141 ccgcccagag ccgtatatct tctcaggttt gatttcaccc ttgctataag ctccagcgga
     1201 ttaaagggct ttgttatata gtcatctgct cccatattga gccccaggac tatgtcagat
     1261 tcttcggatt tggcagacag cataataacc gggatgtttt taatttccct tatttttgtt
     1321 gtagctgtta tgccgtccat ttccggcatc attatatcca taattattaa atgcacttca
     1381 tttttttcag ctgtttcaat tgcctgcttt cctgaataac atttaaaaat attgtattct
     1441 tcgcttttta aataggcttc aattgcatcc acaatttctc tgtcgtcatc acagactaat
     1501 atgttcatgt tattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncaattg
     1621 catccacaat ttctctgtcg tcatcacaga ctaatatgtt catgttattt ctcctgtcaa
     1681 ttaaatctat tcaccattcc gaacctaaag ccgtttccct tctataaagg tataagattc
     1741 ggcaggttag agcatttcac agtttcatta accaatgtgt ttactcactt tggttggaga
     1801 aattgagtag tgaacctaaa atttaatatt taatcatact atataattct taaaatttaa
     1861 ttaaaaaacg gggtggtgtc ccccgcccaa atttacctgt taaactttat ggtgcgtcca
     1921 attatttgga ctaaatgcag cttctgctta gttaacacag attttcaaaa aaacctaggc
     1981 ttcattgaag gcttgcaaga gccttttccg cccaagagga atctttcaaa acagcccttc
     2041 caacacctat gaaatctgcc ttgctttctg ctaagaggtt ttcggcactt tgtggctgcg
     2101 taattccacc tgtcaatatt acaggtattc caacttcttt tttaattatt tccgattgaa
     2161 gggaaaagta tccctgctca tttgagttgc ttggaatata accgcacata ccgcctgata
     2221 tgtctaacat atccactcct gctttctcaa aacacgccgc agcttgaacc gcatcatcca
     2281 tagtgagccc gtcttccctg tcatcagtcg ctccaagtct taacagtaaa ggataatcat
     2341 ctcccacagc ttttctgaca gcttcaataa tttcaaggtg tattttaatt cttccatgga
     2401 tgtcaccgcc gtattcatca gttcttttgt ttgataatgg agacaaaaat tgatctaaaa
     2461 gatagctgtg ggcagaatgg atttcaactc cgtaaaatcc tgcttttttt actcgtattg
     2521 cagcattttt aaaattgttt attatttctt taatattgtc ctttgtaagc tctgttggaa
     2581 ctaaatctgt aagcttcctg ggattcagta ttgcagaggg accgacaggt ttttggttta
     2641 cttccatcct tgcattgctt cctgcgtggt taatctgcat cactgccttg ctgccgtttt
     2701 tatgaattgt ttcggctaat ttttttaatc cttcaatcat gctgtcatcc tctacagaca
     2761 gctggtttaa agatgctttc ccatcccttg aaatatagct gtgctcaatt atgactagtg
     2821 atatgtaacc gccctttgat ttttcctcat aataatccaa tacatcccgg cttacacttc
     2881 catctacatc tgattttgca gtagccattg gcggcatcac aagcctgttt ttcaatttaa
     2941 tttttccatt gtcaaatggt tttaacaaat aagacatatc ctacctcctt ttttatgaca
     3001 ctactcccgt tcccttgtca tttgaattta ttaagtccgt ttctgactgc gtttataact
     3061 gtatcctccg aatcaattgt gtaatcccgg gatactgtta tttcttcctt tgtcttgtca
     3121 tagtctcttc cgttccataa tattgctatt tttatccggt cgtatttata aattatatta
     3181 agcatatctt caaaccactt agctttatct cctcctaaag ctgaagagct gaattccgtt
     3241 atcatcatgg gatgagtaaa acgctcaata tagtcgtaat aaaaatggtc atatgcttcc
     3301 gggaaacttc tccatatttc ccctttgtaa tagctgccgg tattgtatga tgtcaatcct
     3361 acaacatcaa catatttgtc cccgggataa tagcataaat agttattgta ggagaaatca
     3421 ggaaatgatt tttcgtttgg attccaaacc catattaaat tatcaacccc atggtcttca
     3481 aatttatcat atatgtacct ccagcagtca ataaacaaat ccgtatcctt tccaaccttg
     3541 tgagaacagt ataaaaccca ttcaccgttc atttcattat ttggccgaaa cagtacagga
     3601 taatcatatt cgttgaattt ctgtgctaat tcatccaaat atctatcgta tttcccttcc
     3661 agaatatcca aggttatatc tttttcctta tcgtttatta tgtcagttgt atataaaccg
     3721 tattccacaa ccttccctga ttcctttgcc ttgttcatag aatcagtttt aaagggcagg
     3781 gaaaaattgt tgtacaaaag agttaccggg aagttgtaat ccagcatttt ttctatctgt
     3841 tgaagcctgt atgggtactt gggaaatgta ggttcgaata ttccaaagtc tactttttca
     3901 ttatttataa aatattgatc ataaaattcc tgagtcagct catcaaaatt cttctgttca
     3961 ggattaaaaa ctgcatcctt tttaagtatt tctgtctttt ctataaattt aaattccggt
     4021 aaaatttcat caatattcaa aggtgaagat gatttcataa aaacagttat tgattcataa
     4081 gtatctcttg caaatgttac agttccgtaa taattcctgt ccagctgatc actttttatc
     4141 ttccgtcttt taaacaatgt tacatgacct gaaagtccgg cataactaca atcgtattca
     4201 tctataattg caaattcagg atttctgaat attcctctat taccgtagta acggtaggta
     4261 gaatgggaat tgaccgtgtt gtaaaaatta tcatagaaga tttccactac caggttgtct
     4321 gattcaaatc tgcttttcac gctgacaata tcttcattta gctttaggtt gccggggatt
     4381 attatttcat atccgtaggc attgtttctg tatttattgt attggggaga ataaattgtt
     4441 aattctgtgc ttttgtcgat ttccggaagt cctgagtttt caggttctaa gatatctact
     4501 atgccgggaa ggtccaatga cttaactatt tttgaagctg cagtatcctc cccgttgacg
     4561 ggcgtaaatg taagtgcgaa aattaatcca aatattattt ctctcatgta attctccttg
     4621 gtcttatttg taatttaatt ttagcaaata taaaattttt agtcaaacac caattgttat
     4681 taaatgattg ttgtgctata attcattttg tgcaaggagg tagctatggt tactgcagat
     4741 acttttaaac gaggatttaa aaacagtatt tatacactta tagagctttc aaaaatatta
     4801 attcctgtat atattggtgt tgaactgctt tctgtatcag gacttttaaa tatattggct
     4861 gaaatattca aaccattaat gtcaatattg gggcttcccg gtgaagcttc actggttata
     4921 atattagggt actttttggg aacatatgca ggcttgggtg taattgcagc cattgaactt
     4981 aatacagttc aaattacaac actggcaatc atgatttcaa tttcacacag tttgatttct
     5041 gagtccgcag tagtgaaaaa attaggtgtc agtgcagctg caagtgtggc attaagagtt
     5101 tcttcctcaa ttttaatggg atttttatat tataggatat tcgggtgata taatgagcgg
     5161 gattttggat attgttttta atttattggg ttttctgtgg aatatagcca gagtattagt
     5221 gccaatcatg attgtgatag aaatatttaa ggatacacat ttaattgata ggatttcaga
     5281 ttattttaat cctgtttcaa agttctttac aataagcaag accgcgggga tatctctttt
     5341 gttcggtatg tttttcggaa ttataatagg ggcgggtgca atgcttcaaa gtgttaagga
     5401 ctataatgtg gacaaaagaa gtgtcttttt aatcagcatg tttatatcac cgtgtcatgc
     5461 tatatttgaa gacgcattta ttatgaatgg tgccggtgca aatattgtgc cgctgctggc
     5521 ttcaaggttt atttctgcaa ttatggcaac atttatatta tcacgggtaa taaaaaaaga
     5581 gcccctggat gctataagta ataattaaca ttgataaggg cacagaaata gtgtatctag
     5641 tgacaaatag aattcagact gcccaaaatg cttactaaca gtgctccttc ggtccatttt
     5701 gctgtataat gaggtttttg ggctttgatt tgctcaaaat ttcaagtaat gtgtgaatgc
     5761 attcaagaaa aaagataatt ttcttgtaga taatgctaaa aatttcaagt aatttgttga
     5821 ataatagaaa ttaatacagc aaaaatcaca cattacttga aattttgagc atttagttta
     5881 aaaggtattt actaattgat tttcacacaa cctgacgggg tgttgccttc attaaattga
     5941 caaaacaccc gtcatt
//
LOCUS       DAOPUE010000110         5887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13487_length_5887_cov_8.314472, whole genome shotgun sequence.
ACCESSION   DAOPUE010000110 DAOPUE010000000
VERSION     DAOPUE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13487_length_5887_cov_8.314472"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1550)
                     /locus_tag="PKH42_08415"
     CDS             complement(51..1550)
                     /locus_tag="PKH42_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOA20496.1"
                     /translation="MEFFQYAIQAATPENMLWLVIGCIIGAILGALPGMSADTGLAIF
                     IPLTFNLDPTTALITLGAIYVTGAYGGNITAVLVNTPGTSDSVFMTIDGYPMTQRGEG
                     LKAIGVTTFSSFFGGMVGAASLIFLAPIIAKMAVLFGPWELFLVTMMGMVVILGMVKE
                     GVLKGLVSASFGFLCSLIGMDGVTGLTRLSFGIKPILDSLPLLPVVLGLFAVSQMFDL
                     AATHSATIAITDSAIKGSPFLSKKEHKAMFKNNVRSSIVGTIIGIVPGAATTAAAGIS
                     YNMAKKDDPHPETFGKGNPQGLACVSAANNAEVTGALIPLLTLGIPGNGTSALFLGAL
                     LVHGLAPGTQLFAKTPEIAYGMFFGIIMATFAILLIGLYGAPIYSKITKVPNSILIPI
                     IGSLCVLGAFTYRNLFFDTLLILVFGIIGFYMQRSNFPLAPFVLTFVLGKTSEMAFRR
                     TMIITGGDLTSVMFKPLALALFAIDLILLIWPFWGDIKDYLKKDKTKMV"
     gene            complement(1588..2043)
                     /locus_tag="PKH42_08420"
     CDS             complement(1588..2043)
                     /locus_tag="PKH42_08420"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOA20497.1"
                     /translation="MENGYNKKFNSNYLSAAFIYLVSISFLINSFSIKDPQSRMFPTV
                     ICITTIILATLLVIRTKFMTTKKADEDNLTGMTTALKMLALLFLYVVGIEIVSFYIAT
                     PIYLSFTMLALGQKNKKLVVIVSLLFTLGVYLFFDLLLGMAVPMGRFLS"
     gene            complement(2122..3162)
                     /locus_tag="PKH42_08425"
     CDS             complement(2122..3162)
                     /locus_tag="PKH42_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOA20498.1"
                     /translation="MIKRKTISIIALLLVILLSLAMAGCSQKEEPAEKPSENKEGVVP
                     RPEGLPEDYPNKEITFIYAFSAGSPLEAYIRILGDKMQEMQGWKNGVVISFKEGASGR
                     IGWNASAEAEPDGYFTGFAPSAMLISAVSEDVSYGVDKLDFIFNTMSDPGAIGVITGS
                     PYKTLGDLVQAAKENPGKISVGVTSVIGQEGLTIKMIERAAGVDFNEVAFDGEGAIFA
                     AVLGKHLDAFCLNVSDATSFIEDKSITILATGDSDRSPFLPDVPTYKEAGYDVTQVNM
                     RAVSCPKGTPEPIRKYLEECFIAAANDPEVAAKAAEMKLPIDTLPGDEVKAIFTQISE
                     GYAKLWAEDPWN"
     gene            complement(3402..4649)
                     /locus_tag="PKH42_08430"
     CDS             complement(3402..4649)
                     /locus_tag="PKH42_08430"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOA20499.1"
                     /translation="MNRYTEILKEELIPAFGCTEPIALAYAAAKATQVLGHIPERIIC
                     SCSGNIVKNAKSVVVPGTDGLKGIEISVVLGAIAGDPDKELEVLNGIEKNKVAEAKKL
                     AEEGMCQIKLVPNVANLYIKIQSFYEDECAAVTLKDGHLNIIELHKNNDLLYKKDSNS
                     EEEDDDIEIFRFKNIYNYANNVNIEEVKLILDKQIEYNLNIAKEGLENDYGARVGKTI
                     YSKSPNDINEIAKAYAAAGSDARMSGCELPVVINSGSGNQGISVSLPIIIYASSLNSP
                     KEELYRALALGNLIAIYLKRGIGKLSAYCGVSCAAAASAAGIAYLYKEPLNVIEATVS
                     NSLATISGMICDGAKPSCASKIAIAFDSAMLGYELAKGSNNFKGGDGIVKDDIDSTIR
                     SISKMAKDGMKETDNEILNIMLE"
     gene            complement(4667..5656)
                     /locus_tag="PKH42_08435"
     CDS             complement(4667..5656)
                     /locus_tag="PKH42_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOA20500.1"
                     /translation="MNKMLKKERVIAAIHKEEVDRPPFSIWYHVPHVDQDPVALAEEQ
                     IRLARNYDLDFIKMMPFGNYGAHDFGLSVDFFCTKTQPAFERKFAIDDISEWKEIEPL
                     PGSFGSHGKQLLFARQMQKRLKGNDIPYVQTIFSPLTTAKKLAGPRIFNDLREKPEYL
                     HQALKAITETTVDFVKLNIEAGVAGFFFATQCATYDFMTESEYDEFGVKYDLEVINSY
                     KDLTYFNIAHVHGDNTMFNKLAQYPCNCINWHDRWTAPSMSEARKITDKCLLGGINEK
                     FLTKASPDEIYAHIHEGALNAGLRGLMITPGCVAELETPEVNFYAARLAVESL"
BASE COUNT         1738 a         1210 c          938 g         2001 t
ORIGIN      
        1 ctattaattc tttttgaata aatttgtatc taatcctgca gatactaaaa tcataccatt
       61 ttagttttat ctttttttaa ataatcctta atatctcccc agaaaggcca aatcagcaat
      121 attaagtcaa ttgcaaataa tgccagagcc aatggtttaa acataacaga tgttaaatct
      181 cctcctgtta ttatcatggt tcttctgaat gccatttctg aagtttttcc aagaacaaat
      241 gtaagaacaa agggtgccag ggggaaattg gatctctgca tataaaaacc gattattccg
      301 aataccaata ttaataatgt atcaaagaaa agatttctgt aggtgaatgc accaagtacg
      361 caaagtgaac ctattatagg gattagtata gaattgggca cttttgttat ttttgaataa
      421 ataggggcgc cgtacaatcc tataagtaaa attgcgaatg ttgccataat tattccaaaa
      481 aacattccat atgcaatttc cggtgttttt gcaaataact gagttccggg agcaagacca
      541 tgtaccagaa gtgcgcccag aaataatgca gacgttccgt tacctggaat tcccagtgtc
      601 aaaagaggta ttaatgcgcc ggttacttcg gcattgtttg ctgctgatac acatgccaaa
      661 ccctgaggat ttcctttgcc gaatgtttca ggatgaggat cgtctttttt cgccatgttg
      721 tagcttatgc cggctgctgc agttgtagct gcacctggaa caatacctat aattgtacct
      781 acgatagatg atctcacatt atttttaaac attgctttat gttctttttt ggataaaaat
      841 gggcttcctt ttatggcaga atctgtaata gctattgtcg ccgaatgagt tgcagccaaa
      901 tcaaacattt gcgaaacagc aaacaagccc agaacaacag gaagcagcgg caatgaatca
      961 agtattggtt ttatgccaaa gcttaaacga gtcaatcctg ttactccatc cataccgatt
     1021 agagaacata aaaaaccaaa tgaagcactt acgagacctt ttaaaacacc ttccttaacc
     1081 attcccaata ttacaaccat acccatcatt gtaaccagaa acagttccca tggcccgaac
     1141 agtaccgcca ttttagcaat aatcggagcc aggaaaatca aagaagcagc accaaccatt
     1201 cctccaaaga atgaactgaa tgttgtaact ccaatagcct tcaacccctc tccccgctga
     1261 gtcataggat aaccgtcaat tgtcataaat acagaatcag atgtaccggg tgtgtttacc
     1321 agtacagcag ttatattgcc tccataggct cctgttacat agatagcccc taaagtaata
     1381 agagcagttg tgggatccag attaaaagtc aaagggataa atattgcaag ccctgtatcg
     1441 gcagacatgc cgggcagtgc accgagaatg gcacctatga tacacccgat taccagccat
     1501 agcatattct ccggtgttgc tgcttgtata gcatattgaa aaaattccat atttttcaac
     1561 tccttaataa aaaattgttt caaaatttta acttaaaaat cgacccattg gaactgccat
     1621 acccagcagc aaatcgaaaa acaagtacac tcccaatgtg aacagcaatg atacaatcac
     1681 gaccagtttc ttatttttct gtcccagcgc cagcattgta aaacttagat atataggtgt
     1741 tgcaatataa aagcttacta tttctattcc taccacatat aaaaagagta aagcaagcat
     1801 ttttaatgca gtagtcatac cggttagatt atcttcatca gctttctttg ttgtcatgaa
     1861 ttttgttcta ataacaagca atgtagctaa aattatggta gtaatgcaga ttactgtcgg
     1921 gaacatcctg gattggggat ctttaattga aaatgagtta attaaaaatg atatcgatac
     1981 aaggtaaatg aaagcagctg acagatagtt gctgttaaat tttttattat aaccattttc
     2041 catctgaaat cacctcctgt tgattccttt tctaaggagt cttacgactc cttagaaatg
     2101 ttatggataa ttactttatc tttagttcca tggatcctct gcccataact tggcatatcc
     2161 ttcagatatt tgagtaaata tagcctttac ttcatctccc ggcaatgtat ctatgggaag
     2221 tttcatttca gcagcttttg cagctacttc agggtcgttt gcagcagcta taaaacattc
     2281 ttcaaggtat tttctaatag gttcaggtgt acctttagga catgaaacag ctctcatgtt
     2341 aacttgtgta acatcatatc ctgcttcctt gtatgtggga acatcaggaa ggaatggaga
     2401 acggtcagaa tctcctgttg ctaatatagt aattgatttg tcctcaataa agctcgtagc
     2461 atcagaaaca ttcaaacaaa aggcatcaag atgtttaccc aaaacagcag caaatattgc
     2521 gccttcgccg tcaaatgcca cttcattgaa atctactcct gctgctcttt caatcatttt
     2581 tatggttagt ccttcttgcc ctataacaga agtaacacct acagaaattt tacccgggtt
     2641 ttcttttgca gcttgtacaa gatctcctag tgttttataa ggtgaaccgg taataacgcc
     2701 tatagcaccc ggatctgaca tagtgttaaa tatgaaatct aatttatcaa ctccgtatga
     2761 aacatcttca gatacagcag atatcagcat tgcactaggt gcaaatcctg taaaatatcc
     2821 atcaggctct gcttctgcag aggcattcca tccgattctt ccgctggctc cttctttgaa
     2881 gctgattaca actccatttt tccagccttg catttcctgc atcttatcac ctagaattct
     2941 tatataggct tctaagggtg aacccgccga gaaggcatag ataaatgtaa tttctttatt
     3001 aggataatct tccggcagtc cttccggtct tggaacaact ccttctttgt tttcagatgg
     3061 tttttcagca ggctcctcct tttggctgca gcctgccatt gctaaactta acaatataac
     3121 taaaagcaaa gcaattatgg atatagtttt tctcttaatc attttctttc tccttataat
     3181 tattttttaa tctattgtga tacttttatt tgcaatattt atgccataaa tataaagcac
     3241 ttgaaatata tttttgttga aagagcagaa tgtatttatt aaaaaactat ctgccatata
     3301 accaaattat taataaaagc tttttcatca ttgaaaagtt tttcaaattt gaaaattttt
     3361 tcttttttga aaacaaattt tgtttaatat ggttatactt tttactccaa cattatattt
     3421 aatatttcat tatcggtctc cttcattcca tcctttgcca ttttacttat gcttcttata
     3481 gtactgtcta tatcatcttt aactattcca tcgcctcctt taaaattgtt gcttcctttt
     3541 gcaagttcat atccaagcat ggcagaatcg aaagctattg caatttttga agcgcaggat
     3601 ggtttcgctc catcgcaaat catacctgat atggtagcta atgaatttga aacagtagct
     3661 tcaataacat tcagcggctc cttgtataag tatgctattc ctgctgcact agccgccgca
     3721 gcacaggata ctccgcaata tgcagaaagc tttcctattc ctctttttaa gtatattgca
     3781 attaaatttc ctaatgctaa tgccctgtat aattcttcct ttggtgaatt taaacttgac
     3841 gcatagatga taatcggcag cgatacagat attccctggt ttccgcttcc ggagtttatt
     3901 accacaggca attcgcatcc gctcattctt gcatctgagc ctgctgcagc ataagctttc
     3961 gctatttcat taatatcgtt agggctttta ctataaatag ttttgcctac ccttgcacca
     4021 taatcattct ctaagccttc tttagcaata ttcagattgt attctatctg tttatccagt
     4081 attaatttta cttcttcaat attgacattg tttgcataat tatatatatt tttaaatctg
     4141 aatatttcaa tatcatcatc ttcttcttcc gaattgctgt cttttttata tagcaaatca
     4201 ttatttttgt gtaattcaat aatgtttaaa tgtccatcct ttaatgttac ggcagcacat
     4261 tcatcttcgt aaaaagattg aattttaata taaagatttg ccacgttggg aacgagcttt
     4321 atctggcaca ttccttcttc agcaagcttt ttagcttctg cgaccttgtt tttctcaatt
     4381 ccgttaagca cttcaagttc tttatccggg tcaccggcta tggcaccaag aacaacgctt
     4441 atttctatac cttttaatcc gtctgtaccc ggtacaacaa cgcttttggc attctttaca
     4501 atattcccgc tgcagctgca tattattctt tcaggaatgt gccctaaaac ctgggttgct
     4561 ttagctgctg catatgcaag tgctatgggt tctgtacaac caaatgcagg tattaactct
     4621 tcttttaaaa tttcagtata tctgttcata ataatcccct attttcttat aagctttcaa
     4681 ctgctaatct tgcagcatag aaatttactt ccggtgtttc cagttctgca acgcagccgg
     4741 gtgtaatcat caatccccta aggcctgcat tcaatgcgcc ttcatgaata tgggcatata
     4801 tttcatcagg gcttgctttt gttaagaatt tttcgtttat tccgcccagc aggcatttat
     4861 cagtaatttt tcttgcttca ctcatgcttg gggcagtcca cctgtcatgc cagtttatac
     4921 agttacaggg atattgggca agcttgttaa acattgtgtt gtctccatga acgtgggcaa
     4981 tattgaaata agttaaatcc ttatatgaat ttattacttc cagatcatat tttactccaa
     5041 actcatcata ttcggattcc gtcataaaat catatgttgc acattgagta gcaaagaaaa
     5101 atccggcaac tccggcttca atatttaatt ttacaaaatc aacagtagtt tctgttatag
     5161 cttttaatgc ttgatgcaga tactccggtt tttctctcaa atcattaaaa attctaggac
     5221 cagccaattt ttttgctgta gtaaggggac tgaatattgt ttgaacatat ggtatgtcat
     5281 ttcccttcaa tcttttttgc atttgtctcg caaacagcag ctgtttaccg tgtgaaccga
     5341 aagatcccgg caagggttca atttccttcc attcgcttat atcatcaatt gcaaattttc
     5401 tttcgaaggc cggttgggtt ttagtacaaa aaaagtcaac gcttagtccg aaatcatgtg
     5461 ccccgtaatt tccaaaaggc atcatcttaa taaaatccaa atcataattt cttgccagtc
     5521 ttatttgttc ttctgcaaga gctacaggat cctgatccac atgcggtacg tgataccaga
     5581 tggaaaacgg gggcctgtca acctcttcct tatgaatagc tgcaattact ctttcctttt
     5641 ttaacatctt attcatttta tactcctttt aaattattaa agtatatttt gattatatat
     5701 attgcaaatt tcatgccatt aaacatcaaa tcttttttgc ttaggtttac attcattaca
     5761 ccctttatat ttgtggatac attataactc atttaatcac atctgggaaa ttattttttg
     5821 aaaaagtttt tattaatcat agcattagct aagtgtcaaa acctgtaaat tattatgcct
     5881 tgatacc
//
LOCUS       DAOPUE010000111         5799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13761_length_5799_cov_10.535341, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000111 DAOPUE010000000
VERSION     DAOPUE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13761_length_5799_cov_10.535341"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..380)
                     /locus_tag="PKH42_08440"
     CDS             complement(87..380)
                     /locus_tag="PKH42_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HOA20501.1"
                     /translation="MAKFNETVEKLIPTLDKFVPIVARVHGGHHPEFHDVRKIFDVIK
                     EKVKEAGAEKPNLSEEFAELREITDNYTVPGDVCESYEAVYKMLAELDEAYQA"
     gene            complement(521..2161)
                     /locus_tag="PKH42_08445"
     CDS             complement(521..2161)
                     /locus_tag="PKH42_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20502.1"
                     /translation="MQTKYLKRIIAFVIVLSMVFTTVAMAMPYGQAKKLIKNKYSWAQ
                     VQKYVKGRGIMKGYPDGMFRENDKVKRADAIIMIDRAFKLSALIDIINRDYKDVFNDV
                     DKNEYYFDAVHIARLLGITKGKGNNNFHPKQAITIREAILLIERAMDRNKYFDFDDDI
                     DLWKEYEEKELDKSATRGDIAAMLYYVLTGSKYKGVPQEYDLEEISLTIEKDEILRFD
                     DEYGKKDSIIESIEDMVDDLEYVQFTKPLNKDNTFLYYDYNSRSRSNSFVTNRNKYYI
                     DPDDEQPDLSKVSVVPKTSGVLEIEYIAYDEDGESYKGLIKITVEGKYDDLDDITFTG
                     YENTPIKFMISEFKKVFNKEFEYTPDDEISFELPDEKFGTLYFDENKDGELKDAEEIE
                     AKNSFKLKQMNFIYFNPAEDFVDEDEPVEIKYTVEDQEGNLYEGKINITVKSLLGELT
                     IDEDDDFGIEVKDYLDEFDDYDFQKIKFEKADKDDKSTLTIETNTGTRSVIGMELNIR
                     RLQDIEFEPEKDFDGTTFKYTVTTDEGLQFKGLVTVDD"
     gene            complement(2267..2725)
                     /locus_tag="PKH42_08450"
     CDS             complement(2267..2725)
                     /locus_tag="PKH42_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOA20503.1"
                     /translation="MKTEHKEITGKGHNFEYIVSACLCGEKTRYDGKVFVSDKIKRLV
                     DEGKAITVCPEVLGGLKVPRLPCEIKNSRVINIASDDKTEHFVDGAFKTLEIAKKYGI
                     KKAILKEYSPSCGSKYIYDGTFTKTLIPGQGITTRLLQLNDIEVISDQEF"
     gene            complement(2854..3441)
                     /locus_tag="PKH42_08455"
     CDS             complement(2854..3441)
                     /locus_tag="PKH42_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20504.1"
                     /translation="MKNRHIYIILTRTNTVLSNLISIIKNDEYTHASISLNKNLNTMY
                     SFGRKYTYNPFIGCFVKEDLYEGVFGFHNKLKGQVLELNATEEQYDKVVKLLDEFILN
                     SKYLKYNYLGLINSLFNRESICEDRFLCSEFVYYILNKAGIAELNKPRNLVRPQDLLN
                     LKSRIIFKGNLKSKKFIKKYENSFIEKYSAVGTLS"
     gene            complement(3565..4677)
                     /gene="cobT"
                     /locus_tag="PKH42_08460"
     CDS             complement(3565..4677)
                     /gene="cobT"
                     /locus_tag="PKH42_08460"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOA20505.1"
                     /translation="MHVEFYKNFNIKEASEKELESVLNEIIKGIKKPDSISAEGMQKR
                     LDAKIKPLRSLGVLEDIAVQLAGIQGTEFPEVKGKAVLLMAGDHGVVKEGVSAAPQEV
                     TAQLYYSYLNGGGGINVLTGHAGAKLICNDIGICGRLEPPDLMANRIKNGTDNIAEGP
                     AMTREEALRSLLTGAKIASEAIESGINILATGEVGIGNTTPSAALITVLTGCSVEEAT
                     GSGTGLKGDALKHKCNIIKKAIEINKPNLNDPIDVLSKIGGIEIAAMVGAILEAAYQN
                     VPTILDGIISSAAALVAAKFNPIVIEYMIPSHSSEEKGELVALKHMGLEPRLFLNMRL
                     GEGTGAALMFHIVEAAVKIADEMATFETSGVTTGDF"
     regulatory      complement(4809..4983)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5069..5671)
                     /locus_tag="PKH42_08465"
     CDS             complement(5069..5671)
                     /locus_tag="PKH42_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007069805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOA20506.1"
                     /translation="MKYEKIIKALIHNGYKVSYFENSNEAAKYIVDNVKNTTVGFGDS
                     ATLESINLAELLSKNNTVIDPAPYSGDEFNEVAKKALLTDVFFTSVNGVAETGELVNI
                     DGTGNRVAGSLFGHKKIYFVFGTNKIEPNLEKAIWRARNIAAPQNSKRLGYNTPCAVK
                     ADRCYDCSSSERICNTLNIHLKKMKYVEAECIIINENLGM"
BASE COUNT         1672 a         1181 c          774 g         2172 t
ORIGIN      
        1 ttaactgata ttatcattta tatcataata caatatgccg ttaacaatca acggaaaatg
       61 tgattatttt aaaaaacagc ttctgtttat gcctggtacg cttcgtctaa ttcagctaac
      121 atcttataca ctgcttcata actttcgcat acatcaccgg gaacggtgta attatcggtt
      181 atttcacgca gttcagcaaa ttcttcactc aagttgggtt tttctgctcc cgcttctttt
      241 actttttctt tgattacgtc aaatatcttt ctaacatcat gaaattccgg gtgatgccct
      301 ccatgaactc ttgctacaat cggtacgaat ttatccaatg tcggaataag tttttcaact
      361 gtttcattaa attttgccat atttcctcct tcttttctcc ttttttatga tgtctatatt
      421 ctaccaaatt atttgaaaaa ataaattgat ttaaatcaat aattaaaaat aaaaaaagac
      481 tgagcttggt tgtcaaactc agtccttaac cattatttat ttaatcgtca accgtaacta
      541 atcctttaaa ttgtaatcct tcatctgtgg taaccgtgta tttaaatgtg gtaccgtcaa
      601 agtctttttc aggttcaaat tctatatctt gtaatcgtct aatatttagt tccattccaa
      661 ttactgacct tgttcctgtg tttgtttcta ttgttagagt tgacttatca tctttatctg
      721 ctttttcaaa tttaattttt tgaaaatcat aatcatcaaa ttcatccaag tagtctttaa
      781 cttctatgcc gaaatcatca tcttcatcaa tagtcaattc accaagcaga gattttactg
      841 tgatatttat tttgccttca tataaattgc cttcttggtc ttcgaccgta tatttaattt
      901 ctaccggctc atcttcatca acaaagtctt cagcaggatt gaaataaata aaattcattt
      961 gttttaattt gaatgaattc tttgcttcaa tctcttctgc atcttttagt tcgccgtcct
     1021 tgttttcatc aaaatacaag gtcccgaatt tttcatccgg aagttcaaag cttatttcat
     1081 catccggtgt atattcaaat tctttattaa acactttttt aaactctgaa atcatgaatt
     1141 ttataggagt attctcatat cccgtaaatg taatatcatc taaatcatca tatttacctt
     1201 caacagtgat tttgattaaa cctttatatg attctccatc ttcatcataa gctatgtatt
     1261 ctatttctaa gacaccgctt gttttaggca caactgatac ttttgataga tccggctgct
     1321 catcgtccgg gtcaatataa tatttattac ggttggttac aaatgaatta gaacgactac
     1381 ggctgttata atcataatat aagaaggtgt tgtctttatt taagggcttt gtaaattgaa
     1441 catattctaa atcatctacc atatcttcaa tagactcaat aatactgtcc ttttttccat
     1501 attcatcatc aaatcttaaa atttcatctt tttctattgt aagagatatt tcttcaaggt
     1561 catattcttg aggaacacct ttatatttac ttcctgttag aacatagtaa agcatagcgg
     1621 ctatatcgcc tctggtagca gatttatcta attctttttc ttcatattct ttccataaat
     1681 caatgtcatc atcaaaatca aagtatttgt ttctgtccat tgcacgttct attaacaata
     1741 tagcttctct gatagttatg gcttgtttag ggtggaaatt attatttcct ttgccctttg
     1801 ttattcctaa tagtctagct atgtgtactg catcaaaata atattcattt ttgtcaacat
     1861 cgttaaatac atctttataa tccctattga ttatgtctat caaagcactt aatttaaatg
     1921 ccctatcaat cattataatg gcatcggcac gcttaacttt atcattttca cggaacatac
     1981 catcgggata tcccttcatt atgccccttc cctttacata tttttgaacc tgcgcccagc
     2041 tgtacttgtt tttaataagc tttttcgcct gtccgtaagg catagccata gctaccgtgg
     2101 taaataccat ggacaataca attacaaaag caataattct ttttaaatat tttgtttgca
     2161 ttctaaacct ccatgtttga tttatatcat tattttcttg tattacattc ttcgttatct
     2221 atgttaaata tttaggggta tgagatgtta atgtattttt aagcagctaa aattcttggt
     2281 cacttattac ctcaatgtcg tttaattgaa gaagtctggt agttattccc tgaccgggaa
     2341 tcaatgtctt tgtaaaagta ccgtcatata tgtacttgct gccgcaggat gggctatatt
     2401 ccttcaaaat tgcttttttt atgccatatt ttttagcaat ttccaacgtt ttgaaagcac
     2461 catccacaaa atgctccgtc ttatcgtcag aagcaatatt aattactctg ctatttttta
     2521 tttcacaagg aagcctcggc acctttaagc cgcccaatac ttccgggcat accgtaattg
     2581 cttttccttc atcaactaat cttttgattt tatcggaaac aaacaccttt ccatcatatc
     2641 ttgttttttc tccgcaaagg catgcgctta caatatattc gaaattatgt cccttccctg
     2701 ttatttcttt atgctcggtt ttcatattca acaaatcacc ttcttcccct gcatccgtat
     2761 tcatcaatta tatctccata taaaatttaa tatattttac cacatataat aatattttac
     2821 cattttatcg gacttatcca aataattttt tagttatgat aaagtaccca ccgccgaata
     2881 tttttctata aaactattct catatttctt tatgaatttt ttacttttta aatttccttt
     2941 aaagataatc ctgctcttca gatttaataa atcctgtggc cttactaaat ttctgggttt
     3001 gttaagttct gcaataccgg ctttgtttaa aatataatat acaaattcag agcataaaaa
     3061 tctgtcttcg caaatggatt ctctattaaa caaactgttt atcagtccta aataattgta
     3121 ttttagatat ttgctgttta aaataaactc atccaataat tttacaacct tatcatattg
     3181 ctcttcggtg gcattaagtt ctaatacttg tcccttaagt ttattgtgaa atccgaaaac
     3241 tccttcatat aaatcttctt tcacgaaaca cccgataaat ggattatagg tatattttct
     3301 cccaaaacta tacatggtgt ttaaattctt atttaaggat atagaagcat gtgtgtactc
     3361 atcattttta attatgctga taagatttga taataccgta ttggttcttg ttagaataat
     3421 ataaatatgt ctgtttttca taatccctcc tacgtacgga ttgattatgg caaaacagac
     3481 ttaaatatat ataaaaaata tattaatgat tcattaaaat catatttaat ttacttaatg
     3541 aaattatcgc taacttccac aagattaaaa gtctccggtc gttactcctg aagtttcaaa
     3601 ggtagccatt tcatcagcga tttttacggc tgcttcaact atgtgaaaca taagcgcagc
     3661 acctgtacct tcccctaatc tcatatttaa aaataatctt ggctctaatc ccatgtgttt
     3721 caaagctacc agttcacctt tttcctccga tgaatgggaa ggtatcatat attcaataac
     3781 aatcggattg aattttgctg caacaagtgc tgcggcagat gatattattc catccaatat
     3841 cgttggaaca ttttgataag ctgcttctaa aattgcacca accattgcag caatttctat
     3901 cccgcctatt ttagataata catctatcgg gtcattaaga ttaggtttgt ttatttcaat
     3961 tgcttttttg attatattac atttatgctt taatgcatca ccctttaatc cggtaccgct
     4021 gcctgtagct tcttcaacgc tgcatcctgt taataccgtg attaaagctg cacttggagt
     4081 agtatttccg attccaacct ccccggttgc caaaatattt attccggatt cgattgcttc
     4141 tgatgcaatt tttgcaccgg ttaacaggct tcttaaagct tcttcccttg tcatggcagg
     4201 accttcggca atattatccg tgccgttttt tatacgattt gccattagat ccggaggctc
     4261 aagacgacca catataccta tatcattgca tataagcttt gcgcctgcat gtccggttaa
     4321 tacgttaata ccgcctcctc cgttaaggta actataatac aattgagcag ttacctcttg
     4381 tggcgctgca ctcactcctt cttttacaac accatggtcc cctgccatta aaagtactgc
     4441 ttttccctta acttcaggga attcggtgcc ttgtatgcct gccagctgaa cggcaatgtc
     4501 ttctaaaaca cccagacttc taagcggttt tatcttggca tcaagtctct tctgcattcc
     4561 ttcggctgaa attgaatcag gctttttaat tccttttata atttcattta ataccgactc
     4621 taattctttt tcacttgctt ctttaatgtt aaaattcttg taaaattcta cgtgcattct
     4681 ttacctccaa tttcatatat gtaactctca tcaaacaata atagtttccc ggcaaccaac
     4741 aaaaaaatcc cgacataata aaattattat gttcgggact ttaccttcat cgccgtttgc
     4801 tttcaatcga aagacttcta ttcataggca ggtctcctga ctcagactca aacgtccttt
     4861 tactccttcc cggcatatac cagtggtata gtaaaggact aatcttcaca gtgtcgggta
     4921 cgtaccggat tttcaccggt ttccctattc tccttcttag gcacctatga attttattaa
     4981 tgctattcta atacaaagat taaaccatgt caattatttt aattaacact caggctaata
     5041 acagttggat gttatcagta acgctttttt acattcctaa attttcattt attattatac
     5101 actctgcttc aacatatttc atctttttaa gatgaatatt cagcgtgttg catattcttt
     5161 ccgaacttga acaatcatag cagcggtcag cttttaccgc acacggagta ttataaccca
     5221 gacgttttga attttgagga gcagcaatgt ttcttgcacg ccagatagcc ttttctaaat
     5281 taggctcgat tttatttgtt ccgaaaacaa agtaaatttt cttatgtcca aacagcgaac
     5341 ctgctacacg atttcctgtt ccatctatat ttaccagttc tccggtttca gctacaccgt
     5401 tgaccgacgt aaaaaataca tctgtcagaa gcgctttttt agcaacttcg ttaaattcat
     5461 ctccggaata tggtgccggg tctattactg tattattttt tgataaaagc tcagctaaat
     5521 ttatagattc aagtgtagcg gaatcaccaa aaccgactgt agtatttttt acattatcga
     5581 caatatattt tgccgcttca tttgaattct caaagtacga aaccttatag ccgttatgga
     5641 ttaatgcttt aattatcttt tcatatttca tcaaaccttc ctcctcccaa acaatcaaat
     5701 atgttcctgt taaattatac catggcagaa tataaaggac aagaataaag aataagttcg
     5761 acaagatgca acacgtacac cacttagaat tgcaggtct
//
LOCUS       DAOPUE010000112         5783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13810_length_5783_cov_10.217703, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000112 DAOPUE010000000
VERSION     DAOPUE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13810_length_5783_cov_10.217703"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="PKH42_08470"
     CDS             complement(<1..1675)
                     /locus_tag="PKH42_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOA20507.1"
                     /translation="MYDKEKLSHIKEARQQWEENVLAKTLKKAPESKEEFKTVSGSAV
                     NRLYTPEDIADIDYENEIGYPGQYPFTRGYQPTMYRGKPWTMRMYAGFATAEESNERY
                     KYLVSQGSTGLSVAFDLPTQIGYDSDHPLAQGEVGKVGVAIDSLKDMEILFDGIPLDK
                     VSTSMTINAPAAVLLAMYIAVAEKQGVTPDKLRGTIQNDILKEYIARGTYIFPTEPSM
                     RLITNIFEYCSKEVPKWNTISISGYHIREAGSTAAQEVGFTLADGIAYVDAAIKAGLD
                     VDTFAPRLSFFFNSHNDLFEEVAKFRAARRLWAKIMKDRFKAKDEKSMMLKFHTQTAG
                     CTLTAQQPDNNIIRVAMQALAAVLGGTQSLHTNSRDEALALPTTDSVTIALRTQQIIA
                     SETGVTNTVDPLAGSYYIEAKTKEIEEEAMKYITKIDELGGAARAIDLGYIQKEISDS
                     AYKYQMEIESLDRIVVGVNKYQVEEEPPKGLLKVDPVVGEMQKKKIEDTKAQRNNALV
                     NEKLESLRKACEGTENVMPFILDAVREYATLGEICGVMREVFGEYEQAVL"
     gene            complement(1882..3681)
                     /gene="ftsH"
                     /locus_tag="PKH42_08475"
     CDS             complement(1882..3681)
                     /gene="ftsH"
                     /locus_tag="PKH42_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOA20508.1"
                     /translation="MYILIFVVIIMLVQSMVEPPTQVTSISYSDLVVEIQNDNVKTLT
                     FTENEVEGIFKDGKEFTSYIPSLFYFSGAFYDNHLVEKIEAGEIEVIGEPVPPTPLWL
                     QALPTIGMLVLLGVLWYVFMKQSQGGGTGKAMSFGKSRARMVKDDEKKITFADVAGLD
                     EEKEELSEIVDFLKNPAKFVKLGARIPKGVLLVGPPGTGKTYLSKSVSGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFDQAKKNAPCIVFIDEIDAVGRRRGAGLGGGNDEREQT
                     LNQLLVEMDGFSGNEGIIIIAATNRPDILDPALMRPGRFDRQVHVGLPDVKAREEILK
                     IHVRKKPLGDDVNLEIVAKRTPGFTPADLENAMNEAALLTARQNKTVITMDIIEEAIT
                     KVIAGPEKRSKVISDNEKKLTAYHEAGHAVVAKLSSSKDPVHMITIIPRGGAGGFTMY
                     LPEEDKSYRSKSDMEEYIVRLLGGRVAEKLALDDISTGASNDIERATKIARAMVTTYG
                     MSDELGPMSYGTSDDEVFLGRDFNKIRNYSEDVAAKIDREMRRIIDNAYNKTEKLLSE
                     NIEKLHRVAQALLEKETITGKEFEILFDGGSIS"
     gene            complement(3799..4359)
                     /gene="hpt"
                     /locus_tag="PKH42_08480"
     CDS             complement(3799..4359)
                     /gene="hpt"
                     /locus_tag="PKH42_08480"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOA20509.1"
                     /translation="MNNDIKKILIGEKELQSKVAELGAKITEDYKGKDLLIVCVLKGA
                     VVFASDLMREIKLPLDIDFMAISSYGSNTKSSGVVRILKDLNTGIEGRHVLIVEDIID
                     SGLTLAYLAENLRSRRPASVEICTILDKKERRTANLDIKYTGFVVPDEFVVGYGLDYA
                     EKYRNLPYIAVLKEEVYNKTNQAESE"
     gene            complement(4352..>5783)
                     /gene="tilS"
                     /locus_tag="PKH42_08485"
     CDS             complement(4352..>5783)
                     /gene="tilS"
                     /locus_tag="PKH42_08485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOA20510.1"
                     /translation="LWYNLCYIALRKNMHSLIKNNILEKNLISPGENVLIALSGGPDS
                     VFLFHNLRRLRETLSFNLYASHINHMYRGKDAMHDEEFVRNLCDKYGIKLFVKRKNAT
                     EYAKELKVTEEEAGRVLRYNFFKENLREIGEGKVALAHNLNDQAETVLQRIIRGTGID
                     GLSAMSFQKDNLIRPMLNISRYEIMNYLHENNYEYCIDITNTQDIYGRNKIRLNLIPY
                     LEQNFNPNIQSSLYRMSEAMERDKKIIEKYIESKFSEALKEKSDMRIVLDLNLLKALD
                     IGEIGRIIRRGIEELKGNTVNIEMKHIDSAINLISSGKTGKRINLTDGFTVEISYNNF
                     IVNKTVEKVPDFEYNIVLNRLNYIEEINKTLFATVSDTEDMSEVNIKDKDIVSIDYNL
                     IKGSLVARNRRPGDSMIPCGMMGSKKIKDIFIDLKIPKEERDTKLIIADDENIIWLEG
                     YRINDKYKVSAATKKILNISLGRQNE"
BASE COUNT         1525 a         1263 c          843 g         2152 t
ORIGIN      
        1 gcaatactgc ttgctcgtat tcaccaaaaa cttctctcat aacgccgcat atttctccca
       61 atgtagcata ttctctaact gcatcgagta taaagggcat tacattttca gttccttcgc
      121 aagctttcct aagtgactca agtttttcat taactaaggc attgtttctt tgtgcttttg
      181 tatcttctat tttctttttc tgcatttctc ccacaaccgg gtcaactttt aacagccctt
      241 tcgggggttc ttcttctacc tgatatttat taactcctac aactattctg tcgagagatt
      301 caatttccat ctgatattta tatgcagaat ctgaaatttc cttttgaata tagcctaagt
      361 ctatggctct tgctgctccg cccaattcat caatcttagt tatatatttc attgcttctt
      421 cttcgatttc ttttgttttt gcttcaatat aataagagcc tgccaacggg tctacggtat
      481 tggtcactcc cgtttcgctt gcaataatct gctgtgttct tagagctatt gtaactgaat
      541 cagttgttgg aagagcaagt gcttcatctc ttgaattagt atgcaaagac tgggttccac
      601 caagaactgc tgcaagtgcc tgcattgcaa ctcttataat attgttgtca ggctgctgtg
      661 cagtaagtgt acagcctgca gtttgtgtgt ggaatttaag catcatggat ttttcgtcct
      721 tagctttgaa tctatccttc atgatttttg cccaaagtct tcttgcagcc ctgaatttag
      781 caacttcttc aaataagtca ttatgtgaat tgaagaagaa ggacagcctt ggtgcaaatg
      841 tgtcaacgtc aagtcctgcc tttattgcag catcaacgta tgcaattccg tctgccaaag
      901 taaaaccaac ttcctgagct gcagttgagc ctgcttctct gatatggtat cctgaaatac
      961 ttatggtatt ccattttgga acttcttttg agcaatactc gaatatattt gtaataagcc
     1021 tcatagacgg ctctgtaggg aatatgtatg tacctcttgc tatgtattcc tttaatatat
     1081 cattttggat tgttcctctt aatttgtcgg gagttactcc ttgtttttcg gcaactgcaa
     1141 tatacattgc caaaagtact gctgcaggag catttattgt cattgaagta cttactttat
     1201 caagaggtat tccgtcaaac aatatttcca tatcctttaa tgagtcaatg gcaactccga
     1261 cttttcctac ttctccctgt gccaacggat ggtcagagtc ataacctatt tgcgttggaa
     1321 ggtcaaatgc aacagataga cctgtagagc cttggctaac caggtatttg tatctttcat
     1381 ttgattcttc tgcagttgca aatcctgcgt acatacgcat agtccatggt tttcctctat
     1441 acatggtagg ttgatatcct cttgtaaaag gatattgacc agggtatcct atttcgtttt
     1501 cgtaatcaat atctgcaata tcttcaggtg tatataatct gttaactgca gagccggaaa
     1561 cagttttaaa ctcttccttg ctttcgggcg ccttttttaa ggttttcgcc aaaacatttt
     1621 cctcccattg ttgtcttgct tcttttatat gggagagctt ttctttatcg tacattattg
     1681 cctcctttta tctaaatgca taatctcaag ctatatttta atatgaattc actttttttg
     1741 caacactttc gaataaatga ttttaattaa aaatttcttg caaaaataag tgaatcgagg
     1801 tattttacta ataaaatgga acggcagtat gccgttcctt actgtattat ttacaaacct
     1861 tgtattctta ttcgattttt tctaacttat gcttccgccg tcaaaaagta tttcaaactc
     1921 tttgcctgtt attgtttcct tttcaagaag agcctgagca actctgtgca acttttctat
     1981 attttcgctt aatagttttt cagttttatt ataggcatta tctataattc tacgcatttc
     2041 tctatcaatt tttgccgcaa catcttcgct gtaatttctt atcttattga aatctcttcc
     2101 caagaacacc tcgtcatcgc tggttccata agacataggt cctagctcat cgctcatgcc
     2161 gtatgttgta accattgccc ttgcaatttt agttgctctt tctatatcgt ttgaagctcc
     2221 tgttgatata tcgtcaagag ctaatttttc tgcaaccctt cctcccagca atctcactat
     2281 atactcttcc atatctgatt tcgagcggta tgatttgtct tcttcaggaa gatacattgt
     2341 aaatccgcct gctccgcctc tcggaattat cgtaatcatg tgaaccgggt cctttgatga
     2401 tgaaagcttt gctacaactg catggcctgc ttcatgataa gcagtgagct tcttttcgtt
     2461 atcgctgata actttgcttc ttttttcggg tcctgcaatt acctttgtaa ttgcttcttc
     2521 gataatatcc attgtaataa ccgtcttgtt ttgtcttgca gtcaaaagtg cagcctcatt
     2581 catggcattt tccaagtctg caggtgtgaa tccgggagtt ctttttgcaa ctatttctaa
     2641 attcacatca tctcccaaag gtttctttct tacatggatt ttcaatattt cttcccttgc
     2701 ttttacatca ggaagtccta cgtggacttg tctgtcaaat cttcccggtc tcatcaatgc
     2761 agggtcaaga atgtcaggtc tgtttgttgc tgcaatgatg attattcctt cattgcctga
     2821 gaatccgtcc atttcaacca atagttggtt caatgtctgc tctctctcat cattgccgcc
     2881 cccaagacct gctcctcttc ttcttccgac tgcatcaatt tcatctataa atacaatgca
     2941 tggagcattc ttctttgctt ggtcaaataa atcccttacc ctggatgctc ctactcctac
     3001 aaacatttca acgaagtccg aaccgcttat gctgaaaaac ggcacacctg cttcacctga
     3061 aactgatttt gataaatatg tttttcctgt tccgggaggt ccgacaagca gtactccctt
     3121 aggtatacgt gctccaagtt ttacaaattt agccggattt ttaaggaaat caactatttc
     3181 gctaagttct tccttttcct cgtcaagtcc tgcaacgtct gcaaatgtaa tctttttttc
     3241 gtcatctttc accatgcgag ccctgctctt tccaaagctc attgccttgc ctgttccgcc
     3301 cccttgagat tgtttcatga atacatacca caaaactccc aacaatacca gcattccgat
     3361 tgtaggcaat gcctggagcc acaatggagt aggaggtacc ggttctccga ttacctcaat
     3421 ttctcctgct tctatctttt caaccagatg gttgtcataa aatgctcctg aaaaataaaa
     3481 caatgaaggt atataggaag taaactcctt accgtcttta aatattcctt ctacctcatt
     3541 ttcggtaaaa gtaagtgttt ttacgttgtc attttgtatt tcaactacaa gatcgctata
     3601 acttatgctc gttacctgtg tcgggggttc aaccatactt tgaacaagca ttatgattac
     3661 cacaaatatc agaatgtaca tagcgatgct tctaacaaat cctttcaatt aaagcctcct
     3721 cttagtctag gactactata ctataatatt aacatataaa tttaatcaat acaattattt
     3781 aatttagaat aattcctctt attcgctttc agcttggttt gtcttattgt aaacttcttc
     3841 ctttaacacg gcaatatatg gaagatttct gtatttttcc gcataatcca agccgtaacc
     3901 aacaacaaac tcatcgggaa cgacaaatcc ggtatatttg atgtccaaat ttgcggttct
     3961 tctttctttt ttatcaagta ttgtacatat ctcaacagat gcagggcgcc tcgaccttaa
     4021 attttcagca aggtatgcca atgtgagccc cgaatctata atatcctcta caatcaacac
     4081 atgtctgcct tcaatacccg tatttaaatc ctttaatatt ctaactactc cggacgattt
     4141 tgtattcgag ccatagcttg aaatagccat aaaatctata tctaaaggca attttatttc
     4201 tctcattaaa tcacttgcaa aaactaccgc acctttcaac acacaaacaa tcaataaatc
     4261 cttacccttg taatcctctg taatttttgc tccaagttca gctacctttg actgtaattc
     4321 tttttctcca atcagaattt tcttaatatc attattcatt ttgcctcccc agtgaaatat
     4381 tcagaatttt tttagttgca gctgatacct tgtatttatc attaattcta tagccctcaa
     4441 gccatattat attttcatca tctgcaatta tcagttttgt atctctttct tctttcggaa
     4501 ttttcaagtc aataaaaata tcttttattt ttttgcttcc catcatgccg caaggtatca
     4561 tcgaatcccc cggccttctg tttcttgcaa ccaagcttcc ttttattaaa ttataatcaa
     4621 tacttactat atccttgtcc ttaatattta cttcagacat atcttctgtg tctgaaaccg
     4681 ttgcaaataa agttttattt atttcctcaa tgtaatttaa cctgttcaaa acaatattat
     4741 attcaaaatc cggtactttt tcaactgttt tattgacaat aaaattatta tagcttattt
     4801 caaccgtaaa accatcggtt aaattaattc ttttgccggt ttttcccgag cttattaaat
     4861 tgatggcact gtcaatatgt ttcatctcaa tatttacggt attgcctttc aattcttcaa
     4921 ttccgcgcct tataattctt ccaatctctc caatatccag agcttttagc aaatttaaat
     4981 ccaatacaat cctcatgtca cttttttctt taagcgcctc tgagaatttt gattcaatat
     5041 atttttcgat aatttttttg tctctttcca ttgcttcaga cattctgtaa agggaagatt
     5101 gaatgtttgg attaaagttt tgctctaggt atggaattaa attaagtctt attttatttc
     5161 ttccgtatat atcctgagta tttgtaatat caatacaata ttcataatta ttttcatgca
     5221 gataattcat tatttcatat cttgaaatat taagcattgg tcttattaaa ttatcctttt
     5281 gaaatgacat tgcgcttaac ccgtcaattc cggttcctct tatgattctc tgcagtactg
     5341 tttcagcctg gtcgttcaaa ttatgagcaa gtgctacctt gccttcccca atctctctta
     5401 aattttcctt gaaaaaatta tatcttaaaa ctctccctgc ttcttcttcg gttactttta
     5461 gctccttagc atattctgtt gcattttttc tttttacaaa aagtttgata ccatatttgt
     5521 cacaaagatt cctgacgaat tcttcatcat gcattgcatc tttgcctctg tacatatggt
     5581 tgatatgaga agcatacaaa ttaaagctta aagtttccct aagccttctt aaattgtgaa
     5641 acaaaaacac cgaatcgggt cctccggaca aagcaatcaa aacattttct ccgggagata
     5701 ttaaattttt ctctagtata ttatttttaa tcaaactatg catatttttc ctcaatgcta
     5761 tatagcataa attataccac aaa
//
LOCUS       DAOPUE010000113         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_13944_length_5740_cov_10.813720, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000113 DAOPUE010000000
VERSION     DAOPUE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13944_length_5740_cov_10.813720"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKH42_08490"
     CDS             complement(<1..190)
                     /locus_tag="PKH42_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20511.1"
                     /translation="MKNSIKFSRPLVLIFIVAIIGFFVSGIPFRIFAKLSVGDEYKNI
                     YAEYENMNQDINLKSLYEE"
     gene            complement(180..1682)
                     /locus_tag="PKH42_08495"
     CDS             complement(180..1682)
                     /locus_tag="PKH42_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20512.1"
                     /translation="MTMILSVEIDNNIKIIEASKKGKELSVLRCMSIDTACVKDGKII
                     DMEGAVKIINEELKRSEVKTRKAYFVINSTEIMIRTIKLPFLKKNSDILSMLQIELQQ
                     RISADLSKYKIIYEVSNVTDENKIQYAEYIVYCVPLILIGQYIELAAKLKLKLLKIDI
                     SLNCINALYKNNFKINDVVLNNSEIISFFNVSKNSVSFAVANNGLCDFHISSEFENNY
                     IEKSAEPHSQHGYWEVATDDDLFAALIAKFMRCYYSVSGNRSIDKMYIYGSFNQKYKE
                     AIKTKLNLSSEVISTVSDLTVEIPDNFELSKYFNTFLMLLCNNKNLDLNTDRKLKFKN
                     KCRYAAILLIIAAASTALSGFFNSQAVMRNEVTAMSSYIDDGENNQIYSVIEGIKNET
                     DYLKFYLQQAQELKAAAAANDYVDTNILREVYRIKPFETRVTSIYSGRDSTQLLCLSP
                     SMSEAALFFSDLKEIEQVKSAYMPAVQSKSGEPFSYSIVLKLKEVSKDEK"
     gene            complement(1687..2103)
                     /locus_tag="PKH42_08500"
     CDS             complement(1687..2103)
                     /locus_tag="PKH42_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOA20513.1"
                     /translation="MLSAYPACIYKERDGEYSVIFPDLNHLATCGETLDEALYMAVDC
                     LAGYLFEAEKEGMKVNSPSNMNDIDLSAEYDDYAEAFVNIITVDVEEYAKKHFEKSIK
                     KTLTIPLWLNNMAVDKNVNFSQVLQSALKEKLNIRE"
     gene            complement(2145..2336)
                     /locus_tag="PKH42_08505"
     CDS             complement(2145..2336)
                     /locus_tag="PKH42_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOA20514.1"
                     /translation="MVITKFRDLEKIILSDGWKLKSCVGSHFQYVHPVKTGKVTIPNH
                     AGDIPKGVVSSVLKQAGLK"
     gene            complement(2427..3626)
                     /locus_tag="PKH42_08510"
     CDS             complement(2427..3626)
                     /locus_tag="PKH42_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20515.1"
                     /translation="MISYKCNVIDISGNKQRITCAGNSRKDVIDFLKLQEYTVVKIND
                     KKTINLGNISKNVKSKDLAVFCKQTHAMLKAGVTIVSILKILKQQTENRKLRGYIGLM
                     HEELQKGHTFSETLRLRKGAFPETFISMAEAGELSGNFDVIMDRLSIHYEKENKIENK
                     IKSAMTYPIILSIVCIAVVIFLLTSIMPTFVEMYASSGVPLPKLTLVIINIGNLLKNF
                     WFLIVLWIAAVIFITSSILRIHKFRVKKDYLKLQIPLVKNMIIKVAASRFSRTLSTML
                     GSGASLLGALETVSGVTGNLYICSKILEVKEDVRKGLPLSLLLQNQGVFPPMVYYMIK
                     IGEDSGSLEEVLNKTADFYDEEIETAIHRLTTLLEPVMIVIMAIVIGFIVISMVLPMF
                     EMVNTVQ"
     gene            complement(3706..5391)
                     /locus_tag="PKH42_08515"
     CDS             complement(3706..5391)
                     /locus_tag="PKH42_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOA20516.1"
                     /translation="MNSVCSFSKIGEILLNQNEITYAQLNEALKQQHNRKLGEILIKN
                     NYITEIILLRALSEQLKIDVVNLDNYYIDSDAANLLSEITAKRTLSIPLKAWEGKILI
                     AMEDPLNIFYIEDIETETGRKAEIVLACKSQIIESIEKHMGKRSAEKAVEDFDKEKLP
                     VSEETADENDENIISIANSPVVRLINSLISQALKMGASDIHIEPLENRIRVRVRVDGE
                     LQEILTPSKQTQSAIVTRIKIIAGMNIAEKRLPQDGRIEMKIDDTVVDLRISVIPTVY
                     GEKIAIRILDRNNFLKTKEELGFTLSNMEKFNSLLDVPNGIILAAGPTGSGKTTTLYA
                     ALYELNKLNKNIITIEEPVEYKIEGINQLQVNIKAGLLFSTGLRSILRQDPNIIMVGE
                     IRDEETAEIAVRAAITGHMVISTIHTNDAVSTIMRLKDMGIKPFLVAASLRGIVSQRL
                     VKKICNNCREEYAANVAEKSLMGIKNNLMLYKGRGCPKCYYTGYSSRIAIHEVLIIDK
                     AMRSAIQNEGSNDELAAIAEGAGMTTLRDNCRELVLAGVTTIEEFSQVIYEMP"
BASE COUNT         1772 a         1174 c          749 g         2045 t
ORIGIN      
        1 tttcttcata caagcttttt agatttatgt cctggttcat attttcatat tcagcgtaaa
       61 tatttttgta ttcatccccc acagatagct ttgcaaatat cctgaaaggt attccggaca
      121 caaaaaaacc tattattgca actataaata ttaaaactaa tggcctgctg aattttatgc
      181 tatttttcat ctttgctcac ttccttcaat ttcaacacta ttgaatagga aaatggctca
      241 ccggattttg actgaaccgc cggcatataa gcactcttaa cctgttcaat ttccttcaaa
      301 tcagaaaaaa ataaggcagc ttcactcatg gaaggagaaa ggcacaaaag ctgtgtgctg
      361 tccctgcccg aataaattga agttaccctg gtttcaaaag gttttatcct gtaaacttct
      421 cttaaaatat ttgtatcaac ataatcattt gctgccgctg ccgccttaag ctcttgagct
      481 tgctgtaaat aaaatttcaa ataatccgtt tcatttttta tcccttcaat cacactgtaa
      541 atttgattat tttccccatc atcaatatat gaactcattg cagtaacttc atttctcatg
      601 acagcttggg aattaaaaaa tccggataac gcggtggatg ctgctgcaat tatcaataaa
      661 attgcagcat aacggcattt atttttaaac tttaatttcc tatctgtgtt tagatctaaa
      721 ttcttgttat tacaaagcaa cattaaaaag gtgttgaagt atttacttaa ctcaaaatta
      781 tcaggaattt ctactgttaa atcggataca gtgctgatta cttcactgct taaattcaac
      841 ttagttttga ttgcttcttt gtatttctga ttaaaactgc cgtaaatata catcttatct
      901 atagacctgt tcccgctgac cgaataataa catcgcataa acttagcaat tagtgctgca
      961 aataaatcgt catcagttgc aacctcccaa tacccgtgct gagaatgtgg ctctgcagac
     1021 ttttcaatat agttattttc aaactcagag ctaatatgaa aatcacaaag accattattt
     1081 gcaactgcaa aggaaacaga gtttttactt acattgaaaa atgaaataat ttcagaatta
     1141 ttcaaaacaa catcattaat cttaaaatta tttttataca atgcatttat acaattgagg
     1201 gaaatatcaa ttttcaataa ttttagtttt aattttgcag ctaactctat atattgaccg
     1261 attaaaatca aaggaacaca ataaacaata tattccgcat attgtatttt attttcatca
     1321 gtaacattag atacttcata gattatttta tacttactta aatctgcaga aattctttgt
     1381 tgcagctcta tttgcagcat ggacagaata tccgagtttt tctttaaaaa aggcagctta
     1441 attgttctaa tcataatttc ggttgaatta attacaaaat aagccttcct tgtttttact
     1501 tcgctccttt ttaattcttc attaattatt ttgacagccc cctccatgtc aatgattttc
     1561 ccatccttaa cacaggcagt gtcaatggac atacatctta aaacagacaa ttctttaccc
     1621 ttttttgacg cttcgataat ttttatatta ttatctattt caacagataa aatcattgtc
     1681 ataatatcac tccctgatat ttaacttctc ctttaatgcc gattgcaata cttgagaaaa
     1741 attaacattc ttatctacag ccatattatt taaccacaat ggtattgtaa gagttttttt
     1801 aattgatttt tcaaagtgtt tctttgcata ttcttcaaca tccacagtaa taatattaac
     1861 aaatgcttca gcataatcat cgtattctgc cgataagtct atatcattca tatttgaagg
     1921 agaatttact ttcattcctt ccttttcagc ttcaaacaaa tatcctgcaa ggcaatcgac
     1981 tgccatatac aaagcttcat ctaaagtttc accgcaagtt gccaaatgat ttaagtcagg
     2041 aaaaataacc gaatattctc catccctttc cttataaata cacgccggat acgcagacaa
     2101 cataataact cacctttcct tattttaata gtatgatata ctcatcattt caatcctgcc
     2161 tgttttaata cagaactgac aactcccttt ggtatatcac ccgcatggtt cggaatagtt
     2221 actttccccg tttttacggg atgtacatat tgaaaatgtg acccaacgca tgattttaat
     2281 ttccacccgt cagataatat tatcttttct aaatctctaa atttcgtaat aaccactcca
     2341 ttattattat attatcatat acgtattgtg tcaatacgta tccagatttt agggacaata
     2401 aatataactc aactattcag gcatgctcat tgcacagtat taaccatttc aaacataggc
     2461 aagaccattg atatcacaat gaaccctatt acaattgcca taatcacaat cattacaggt
     2521 tccaacaatg ttgttaatcg atgaattgca gtttcaatct cctcatcata aaagtcagcg
     2581 gtcttgttta atacttcttc taaagagcct gaatcctctc cgattttaat catgtaataa
     2641 accattggcg ggaaaacccc ttgattttga agtaaaagag aaagtggtaa tcctttcctt
     2701 acatcttctt taacctctaa aatcttgctg caaatataaa gatttcccgt tactcccgaa
     2761 acagtttcca atgcaccaag aagtgaagca ccactgccta acatagttga caaagttctt
     2821 gaaaacctcg aagcagcaac ctttataatc atatttttaa ccagtggaat ttgaagtttg
     2881 agataatcct tttttaccct gaatttatgt attctcaaaa tagaagaagt aataaaaata
     2941 acggcagcaa tccatagaac aatcaaaaac caaaaattct ttaataaatt gccgatattt
     3001 attattacca atgttaattt aggaagcgga acaccggaac ttgcatacat ttcaacgaat
     3061 gtaggcatga ttgatgtaag aaggaatatt accaccgcaa tacaaacaat ggaaagaata
     3121 attggataag tcattgcaga ttttattttg ttttcaattt tattttcttt ttcataatga
     3181 attgaaagtc tgtccataat tacatcaaaa ttaccgctca attctccggc ttcagccata
     3241 cttataaaag tctccggaaa tgcacctttt ctcaacctca atgtttctga aaatgtatgt
     3301 cctttttgca gctcctcatg cattaaccct atgtaccctc ttaattttct attttcagtc
     3361 tgctgcttta atattttcaa aatgctgaca atggtaactc ctgcctttaa cattgcatgt
     3421 gtttgcttgc aaaatacagc caaatccttt gactttacat tcttcgagat gtttcccaaa
     3481 tttatagttt ttttatcgtt aatttttaca actgtatatt cctgcaactt caagaaatca
     3541 attacatcct ttcgagaatt acctgcacag gtaatccttt gcttattccc tgatatatct
     3601 ataacattgc atttgtacga aatcataatt actcctcatg agctccttcg gcttcgcctc
     3661 aggatgacaa attacaaatg gggttagggc atctgacaaa tggggttagg gcatctcgta
     3721 aatcacctgt gaaaattctt ctatggtcgt aacacctgcc aataccaatt ccctgcaatt
     3781 atccctcaat gttgtcatcc ctgccccttc ggcgatggca gcaagttcat cattacttcc
     3841 ttcattttga atagcagatc tcatggcctt atcgattatt aatacttcgt gtatagcaat
     3901 tctactgctg tatccggtgt agtaacattt tggacatccc ctgcccttgt acagcattaa
     3961 attattttta atccccatca aactcttctc agccacatta gctgcatact cttccctaca
     4021 gttattacat atttttttta ccaatctttg agacacaatt ccccttaatg aagcagcaac
     4081 caaaaatggc ttaattccca tatcctttag acgcataatt gtagacacag catcattggt
     4141 atgtattgtt gaaatgacca tgtgccctgt tatagctgcc ctcacggcaa tttccgccgt
     4201 ttcttcatcc ctgatttcgc ctaccataat aatattagga tcctgtctta atatagacct
     4261 taatcctgta gaaaacaaaa gtcccgcctt aatattgact tgaagctgat taatcccttc
     4321 aattttgtat tccacaggct cttcaattgt aataatattt ttattcagct tatttaattc
     4381 atacaaagcc gcatataacg ttgttgtttt gccgcttccc gttggtcctg ccgccaaaat
     4441 tattccattt ggaacatcca ataatgaatt aaacttttcc atattgctca atgtgaatcc
     4501 taattcctct tttgttttaa gaaaattatt cctgtccaat attcgaattg caattttttc
     4561 accatacaca gttggaatca ctgaaatacg aaggtcaaca accgtatcat caattttcat
     4621 ttcaattcgc ccgtcctgag gcagcctctt ttccgcaata ttcattccgg ctattatttt
     4681 aatccttgtt actatcgctg actgtgtctg ctttgaagga gtaagaattt cctgcagctc
     4741 accgtctact ctgaccctta cccttattct gttttctaat ggctcaatat gaatgtcgct
     4801 tgctcccatc ttcagtgctt gactgattaa tgaatttatt aatcttacaa caggtgaatt
     4861 agctatgctt ataatatttt catcattctc atccgccgtt tcttctgaaa ccggtaattt
     4921 ttccttgtca aaatcctcaa ctgccttttc tgcacttctt ttgcccatgt gcttttcaat
     4981 tgattcaata atctgagatt tacaagcaag cacaatttct gcctttcttc ctgtttcagt
     5041 ctcaatatcc tctatgtaaa atatattcag aggatcctcc attgcaataa gtatttttcc
     5101 ttcccatgct tttagcggta tggataaagt tcttttagct gtaatttctg acaataaatt
     5161 tgccgcatca gaatcaatat aataattatc taaatttaca acgtcaattt ttaactgttc
     5221 ggataatgcc cttaacaaga taatttccgt aatataatta tttttaatca atatttcccc
     5281 gagcttccgg ttatgctgct gtttcaaagc ttcattcaac tgtgcatacg ttatttcatt
     5341 ttgattcaat aagatttccc caatctttga aaaactgcat acgctattca taaattcact
     5401 ctcttcaagc tatatttgtt tagttataac ttattatgta tctttagggg tttcattata
     5461 ttattcaacc acacctgtgt caatttaact aaacgtttta ttgattacag taaaagctac
     5521 atttaattac aatcaattaa tacaaataaa gtaaatgctg ttatgatttt ccatttatgt
     5581 tatgaacttt ttataacctt atacaataaa aaatgacgat tttcgcgtca ttgtaatgaa
     5641 taagcggaca aatgaagatt taagcggtac ctatctatat tgattgggtc aagtcctttt
     5701 tttggtgtaa attcattcgg taagaatgta atataaaata
//
LOCUS       DAOPUE010000114         5702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14072_length_5702_cov_8.954135, whole genome shotgun sequence.
ACCESSION   DAOPUE010000114 DAOPUE010000000
VERSION     DAOPUE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14072_length_5702_cov_8.954135"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1421)
                     /locus_tag="PKH42_08520"
     CDS             complement(21..1421)
                     /locus_tag="PKH42_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOA20517.1"
                     /translation="MEIKYFDAFIEAMELINDGIQIIDASGKIIYHNPAAKQLDLIDA
                     EKTLGRHILEVYPSLTFETSTIMNVLKNGKPIYNVEQNFVNYKGDKISTLNSTLPIIY
                     NNKVIGALEISRNITEVRELSERIVNLQKELYEANKGEKKKSKPLAKYTFLDIIGQNK
                     EILKLKSLALKAAMTDSPVIVAGSTGTGKELFVQSIHNSGDRKYKPFIAQNCAALPAN
                     LLESILFGSVKGSFTGAENRPGLFELADGGTLFLDEINSMPLDLQTKLLRVIQDGRVR
                     RVGSGKTTDVDVRIISAINTDINTVVETQQIRQDLFFRLSVITLVIPDLCKRIEDIPL
                     LVEHFIDKYNKKYNKFFSGVSKEVLHIFLNYSWPGNVRELEHAIESAISLYDGEIIRE
                     EHLPFQFKNYQPKNDNSSDTGSIQPLNIAVEKVEREIIISALEQTDYNITKAAKLINV
                     PRQTLQYKIKKLGLLF"
     gene            complement(1577..1996)
                     /locus_tag="PKH42_08525"
     CDS             complement(1577..1996)
                     /locus_tag="PKH42_08525"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOA20518.1"
                     /translation="MENVKVRINTIQTIDEAGNEDVIELITEAKLEKLKDCFVINYDE
                     SEITEHKGSRTRLKIYKDKMLMTKVGAFSSKMEFRQGKKYRNLYSTPFGNFDLEYTTV
                     LYNNNLNEKGRGSIHAEYMIAFGDTDKSYNKLNIEIM"
     gene            complement(2008..2814)
                     /gene="murI"
                     /locus_tag="PKH42_08530"
     CDS             complement(2008..2814)
                     /gene="murI"
                     /locus_tag="PKH42_08530"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOA20519.1"
                     /translation="MDNRPIGIFDSGIGGLTVMREVMEQLPYENIIYLGDTARIPYGS
                     KSEQTIKKFSLQCATFLKNKDVKTIVIACNTASSNALDLLTEEFDIPVIGVIDPGARS
                     ASKATKTGKIGVIGTTATINSYAYQKKIMEYMNNAEVIGIPCPLFVPIVEEGWEYSQV
                     AELTAEKYLTELIEHDVDALVLGCTHYPILRHTIKKVLGPQVTLVNPAFETAKDLKNL
                     LIEKDILNENYDKPRYEYYATDAPERFRRIGGNFLKKEIDNLYEIAIDNL"
     gene            3241..4278
                     /locus_tag="PKH42_08535"
     CDS             3241..4278
                     /locus_tag="PKH42_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOA20520.1"
                     /translation="MKKGNPYGTHRVIEPKGVLPQPALKIDNTMEIFDNEILIDVQTL
                     NIDSASFTQISDQAGGDVEKIKEIMKGIVAERGKHQNPVTGSGGMLIGTIEQIGPALE
                     GKTDLKVGDKIATLVSLSLTPLVIEEIFEVRKDIDQVDIKGKAILFESGIYAKIPEDI
                     PENLALSVLDVAGAPAQTRKLVKEGDTVLVLGGGGKSGLLCLYEAKKKAGPTGKVICW
                     AHREVSLNTVRELGLADVLISGDATNATEVYEKIMEATDGQLCDLVISVVSRPNCEMS
                     AILATKDEGTVYFFSMATSFTKAALGAEGVGKDINMIIGNGYTKGHSETALQIMRESK
                     GLRELFTKLYA"
     gene            4359..5609
                     /gene="ablA"
                     /locus_tag="PKH42_08540"
     CDS             4359..5609
                     /gene="ablA"
                     /locus_tag="PKH42_08540"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOA20521.1"
                     /translation="MAYYNEIELWKDVKPQEWNDWRWQVENRIDTIEKLKKIINLTAQ
                     EEEDISKVLEKFRMGITPYYAAHMDKDDPRDPIRMQAVPTFVETHVSAADMVDPLHED
                     TDSPAPGLTHRYPDRVLFLITDQCSMYCRHCTRRRFAGQSDDEVGMENIDKCIEYIRK
                     TPQVRDVLLSGGDCLLVSDKKLEYIISKLREIPHVEIVRLGSRTPVVLPQRITDDLVN
                     MLKKYHPIWLNTHFNHPKEFTPESMEALRKLADAGIPLGNQSVLLRGVNDCPHIMRDL
                     VHKLVMNRVRPYYIYQCDLSLGIEHFRTPVAKGIEIMESLRGHTSGFCVPTFVVDAPG
                     GGGKTPVGPNYVLSQSADKVILRNYEGVITTYTEPKLPDLECRCDVCQGKKTDPLTGV
                     AGLLQGNDLALEPTKLARRERTHH"
BASE COUNT         1800 a         1028 c         1069 g         1805 t
ORIGIN      
        1 aggtacgtcc ccattgtcac ctaaaagaga agccctaatt ttttgatttt atattgcagc
       61 gtttgtcttg gaacatttat aagttttgca gcttttgtaa tattatagtc tgtttgctcc
      121 aatgcagaaa tgataatttc cctctcaact ttttcaacgg caatatttaa aggctgaatt
      181 gacccggtat ctgatgaatt atcgttcttc ggctgataat ttttaaattg gaaaggaaga
      241 tgttcttctc ttataatttc accgtcatac agcgatatgg cgctttcaat ggcatgctca
      301 agctctctta cattgcccgg ccagctgtaa tttaaaaata tatgtaaaac ttctttagaa
      361 actccactga aaaacttatt gtatttctta ttatatttat ctatgaagtg ctctaccaat
      421 agaggtatat cttctattct tttgcataaa tcaggaatca ccaaggttat aacactgagc
      481 ctgaaaaata aatcctgtct tatttgttgt gtttctacca ctgtattgat atccgtatta
      541 atagcggata taattctaac atcaacatcg gttgttttcc cggagcccac tcttctgacc
      601 cggccgtctt ggattactct gagaagcttt gtttgcaggt ctaaaggcat agaatttatt
      661 tcatccaaaa ataaggttcc accgtcagct aattcaaata agcccggcct gttttccgcc
      721 ccggtaaaac ttccctttac agagccgaac agaatacttt caaggaggtt agcaggcagg
      781 gctgcgcagt tttgagcaat aaaaggtttg tatttcctgt ctccggaatt atgtatggac
      841 tgcacgaata attctttgcc ggttccggtg ctccctgcaa ctattacggg ggaatccgtc
      901 attgcagctt ttagggcaag agatttaagt ttaagaattt ccttgttctg acctattata
      961 tccaaaaaag tatatttggc taatggtttt gacttttttt tctcaccttt atttgcttca
     1021 tacaactctt tctgcaggtt tacaattctt tccgacaatt ctcttacctc agtaatattt
     1081 cgtgaaattt ccaaggctcc tataacttta ttgttataaa taatgggaag ggttgagttt
     1141 aatgtagaaa ttttatcacc tttgtagtta acaaaatttt gttcaacatt ataaataggt
     1201 ttaccgtttt tgagtacatt cataatggta ctcgtttcaa aagtaagaga aggatatact
     1261 tcaagtatat gtctacctaa tgttttttcg gcatctatta aatccaactg ttttgctgca
     1321 ggattgtgat aaattatttt tccgcttgca tcaataattt gaataccatc atttattaat
     1381 tccattgctt ctataaaagc atcaaaatat ttaatttcca tactttcctc tttcttgccg
     1441 aaaattaagc atagtatgcc gaaaaacagg cagcaatgtt cgtcttgtgc gcaatttggg
     1501 gacattcctc ttacaccaaa gactatccct aatagaggtg tgtcccatac cttgtacaaa
     1561 tggggttagg gcatctctac attatttcaa tatttagttt attataactt ttatcagtat
     1621 ctccgaaagc tatcatatat tcggcgtgta tactgcctct tcctttctca ttcaaattat
     1681 tattatataa gaccgtagtg tattccaaat caaaattacc gaatggagta gaatataaat
     1741 tacggtattt tttgccttgc ctgaattcca ttttagaaga aaaagctccg acctttgtca
     1801 ttagcatttt atctttatat attttcaatc tggtacggct tcctttgtgc tccgttattt
     1861 cactttcatc atagttaata acgaaacaat cctttaattt ctctaatttt gcttctgtaa
     1921 tcagctcaat aacatcttcg ttgcctgctt catcaattgt ctgtattgtg tttattctga
     1981 ctttcacatt ttccatttta tatcccctta aaggttatct atggcaattt cataaagatt
     2041 atcaatttct ttttttaaaa agttgcctcc tattcttcta aaacgctccg gtgcatcagt
     2101 tgcataatac tcatatcgcg gtttatcata attttcattg aggatatctt tttctatcaa
     2161 aaggtttttt aagtccttgg cagtttcaaa agcaggattt acaagtgtta cttgaggccc
     2221 taaaactttt tttatagtat gcctcagtat aggataatgg gtacatccta aaaccaaagc
     2281 atcaacatca tgctcaatta attccgtcaa gtatttttcc gcagtaagct ctgccacttg
     2341 agagtactcc cagccttctt caacaattgg tacaaaaagc ggacaaggta tgccgattac
     2401 ttcggcgtta ttcatatatt ccattatttt tttctggtat gcataggagt tgatggtggc
     2461 tgtagtgcct attactccta ttttgccggt tttggtagct tttgaagcac tccttgcacc
     2521 ggggtctatt actccgataa ctggaatatc aaactcctcg gtcaataagt caagcgcgtt
     2581 tgagcttgct gtattacatg ctataacaat ggtttttaca tctttatttt ttaaaaatgt
     2641 agcgcattga agtgaaaatt tctttattgt ttgctcagat ttagagccgt agggtattct
     2701 tgcggtatct cctaaatata ttatattttc gtagggaagc tgctccataa cttctctcat
     2761 tactgtcagt cctccgattc cggagtcaaa aattccaatt ggtctgttat ccattttata
     2821 ccatcctata cttattaaat ttatcttttt actatatttt attaaaagat tgttatataa
     2881 tgtatgatat acataatcat ttgatatttt actatgctaa gaattatcat atcgtctcag
     2941 gccatgaata cgctttaatg cggcttgctt cacaccgata caataaattc atcaaatcat
     3001 ataaaaccat tttggtttta tcaccttttg aatttattat atcataatat tatttactca
     3061 tcaacaaaaa gctgaatttt cggcacttac ctgacaatga aaacattttt ataaagcaaa
     3121 tgttggaaac tggctgtttt ttatttttgg ataattggca taaattttgc taattatata
     3181 tttgaatata cttaatattc catacaatcc atacccaatt attgaaaatt tggaggcaat
     3241 atgaaaaaag gaaacccata cggcacacac agagtgattg aacctaaagg agtgttacca
     3301 caaccggctt tgaaaattga caacacaatg gaaatatttg acaacgaaat tcttattgat
     3361 gtgcagacat tgaatattga ctctgctagc ttcacgcaaa tcagcgatca agcaggcggg
     3421 gatgtagaaa aaatcaaaga aattatgaaa ggtatcgttg ccgaaagagg aaaacatcaa
     3481 aaccctgtaa caggttcagg cggaatgtta atcggtacta ttgagcaaat aggaccagcg
     3541 cttgagggaa aaacagattt gaaggttgga gataaaatag caacactggt atcattgtcg
     3601 cttactccat tagtaatcga agaaattttt gaggttagga aagatattga ccaagttgat
     3661 atcaaaggga aggcaatatt gtttgaaagc ggaatttatg caaagattcc tgaagacatc
     3721 cccgaaaatc ttgctttgtc agtgcttgat gtagcaggag caccggcgca aaccaggaaa
     3781 ttggtcaaag aaggagatac cgtattagtt ctcggcgggg gaggcaaatc aggtctttta
     3841 tgcttatatg aagcaaagaa gaaggcaggt cccacaggaa aagttatttg ctgggcccac
     3901 agggaagtat cactgaatac agtaagagaa cttggattgg cagatgtttt aatatcaggc
     3961 gatgctacaa atgcaactga agtatacgaa aagattatgg aagcaacaga cggacaatta
     4021 tgtgatctgg taataagcgt agtatcaaga ccaaattgtg aaatgtcggc aatacttgct
     4081 acaaaagacg aagggacagt atatttcttc agcatggcta caagctttac gaaagcagca
     4141 ttaggagccg aaggtgtagg caaggacata aacatgataa tcggtaacgg ctatacaaag
     4201 ggacattcag aaacggccct gcagataatg agggaaagca aaggattaag agaattattc
     4261 acaaaattat atgcataaat acataataaa ctaagcaaca tgtcatttgc tcggcgaatg
     4321 acatatgttg cttgtttaaa taattgagga ggtagaatat ggcttattac aatgaaatag
     4381 agctatggaa ggatgttaag ccacaagagt ggaacgactg gagatggcag gttgaaaaca
     4441 gaatagacac aatagagaaa ctaaagaaaa taattaatct tactgcgcaa gaggaagaag
     4501 atataagcaa ggtgttggaa aaattcagaa tgggtatcac accatattat gcagcacata
     4561 tggataaaga tgacccgaga gaccccataa gaatgcaggc tgttccaaca tttgttgaaa
     4621 cacatgtaag cgctgcagat atggttgacc ctttgcatga agatacggat tcgcctgcgc
     4681 cgggattaac ccatcgctat ccggacaggg ttttgttctt aataaccgac caatgctcaa
     4741 tgtattgcag acactgtaca agaagaagat ttgcaggaca atcggatgat gaagtaggaa
     4801 tggaaaatat cgataaatgc atcgaatata tcagaaagac tccccaagta agagacgtat
     4861 tattgtcagg cggagactgt ttgttagtgt cagacaaaaa attagaatat attatcagta
     4921 aattaagaga aataccacat gttgaaattg tgcgtttagg ctcaagaaca cctgtagtat
     4981 tgcctcagcg tattacggat gacctggtaa atatgcttaa aaaatatcat ccgatttggt
     5041 taaacactca ttttaaccat ccgaaagaat ttactcccga atctatggaa gctttgagaa
     5101 agttggctga tgcaggtatt cctttaggaa accaatcagt attgttaaga ggagtcaatg
     5161 actgcccgca tattatgaga gatttggttc ataagcttgt tatgaacaga gtaagacctt
     5221 actatattta tcaatgcgac ctttcactcg gtatcgaaca cttcagaaca cctgtggcta
     5281 agggaattga aataatggaa agcttgcgag gacatacttc cggtttctgc gtacctacat
     5341 ttgttgttga tgcaccgggg ggcggaggaa aaacacctgt aggaccgaat tatgtattgt
     5401 cacaatcagc tgacaaggta attttaagaa actatgaagg tgttattaca acatatacag
     5461 agccgaaatt accggatttg gaatgccgct gcgatgtatg tcaaggcaaa aaaacagatc
     5521 ccttaacagg agttgcagga ctactgcaag gaaatgactt agcacttgaa cctacaaagt
     5581 tggcaagaag agaaagaaca catcattaaa gatgccctaa ccctattttt ccgaccagag
     5641 ggagagaaaa atagcggtag gtcattcgca acgaattccc aatgtatgcg accaaaggga
     5701 gc
//
LOCUS       DAOPUE010000115         5698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14090_length_5698_cov_10.131490, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000115 DAOPUE010000000
VERSION     DAOPUE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14090_length_5698_cov_10.131490"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKH42_08545"
     CDS             <1..1002
                     /locus_tag="PKH42_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOA20522.1"
                     /translation="ITSRYFMEALYADRLRTFIKENYNIVALIDYSGDTAFKRAMISP
                     AVIILSNKWNKNMFLYVKKDRDVIESYKYRQDKLKSSGWIILKDEDEKLFEKIDSISN
                     TYIKDILSIKQGIITGCDKAFIVTEEVIENYKIESFLLKKWIKNSNISKKSIKYNNLY
                     LIYSDNIQNESDCPNAISYLSKYKDKLMSRRECKKGFRKWYELQWGRNKSDYENPKII
                     FPYKSKQNNFYFDEEAFFCSADIYLMNEFSKGLSLNYLLSYLNSEIFEFYLKCQLKKV
                     GRNIYEYYPYKLNNLKVYLPQGDIAQKFSRMENISIEIFKKLFNINDKEVNIIKNYI"
     gene            1020..2639
                     /locus_tag="PKH42_08550"
     CDS             1020..2639
                     /locus_tag="PKH42_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20523.1"
                     /translation="MRKFKKLLLLIIIFIVLSIKPALARTVFYDTFNHWAESDINFAS
                     NTINVFKGYGDFTFRPENDITRAEFITILSRTAYKYNLMKEIYTGDLSYRDMSMKHWS
                     YTFIISMNEYLKTNKFTLQDIFPGSNFYPDKAITREESAALIAAFCKDAIYDNPLRFS
                     DVTTGNKYYNEIQRLCNSGILVGYEDNTFRGNTNITRAEAASLIKRVYSDIKTSTNDN
                     YLTKLEFMPIKGEEMYSYFGNYDLSTTNANDKKFIKAKDTLEYVAFGGYIFPEDSNLY
                     DLNAVKTMESLHASGYFNVAGTNYYMIMFGSYSSEDKSKFANEILTNIIARNDLNDAE
                     LMQIFTLVSKYDVSEALYMGALEKWNNLTSNDNSKANILFFRYAYYIKNDNKDILRAL
                     VYEDLKKNNNIVSLLNISFNSNDTNAIDFRNYNFGNYSFSLYKDTTLFRYLRNMTMPL
                     NTNLRVVELSNLLMIEKNPKPTVNMADYENLFTKYSVNKLYVLNFLGEKERAFVEGIN
                     DYDIIKTFEMYKKNPSIFDDTYTGILKKVKE"
     gene            complement(2694..3902)
                     /gene="pepT"
                     /locus_tag="PKH42_08555"
     CDS             complement(2694..3902)
                     /gene="pepT"
                     /locus_tag="PKH42_08555"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOA20524.1"
                     /translation="MKVHERFLNYVKIDTQSVHDAKKIPSSEKQKDLGRLLVEEMISI
                     GIEDAYMDENGYVYGTVKGNTDAPVIGFIAHMDTSPDMPGSGVKPKIIYNYDGSDIML
                     NDEKQIVMKTEMFEHLSKYIDQDLIVTDGTTLLGADDKAGIAEIMSMAEYLINNPQIK
                     HGTIKIGFTPDEEVGQGAELFNIEKFGADYAYTVDGGEIGELEYENFNAASAKVTING
                     VNIHPGTAKNKMKNSILIGMEFHNMLPVYETPENTEGYEGFSLLTDMSGNVEKTLLEY
                     IIRDHDIAKFNIKKERFQKIADYLNEKYGQGTIELKLEDSYFNMKEKILPHMHIVENA
                     IRAMENVGIEPKVVPIRGGTDGARLSYMGLPCPNLCTGGHNFHGRYEYISIQSMEKVV
                     ELLIEIATVK"
     gene            4213..4848
                     /locus_tag="PKH42_08560"
     CDS             4213..4848
                     /locus_tag="PKH42_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOA20525.1"
                     /translation="MDFIKEGYKVKSATVIKNLEKRNMEGYYCETVEEAVEKAMSMIK
                     QDDTVGWGGSTTIDQIGIKKLLEEKNIAVYDRDKETDPAEKVKMMKKALTSDVFLTSA
                     NAITMDGELLNIDANGNRLAAYCYGPNSVIVVAGMNKIVTDLDIALKKARLDATVPNT
                     FRTNSKAPCHFTGKCIECTMSDTLCGQILITRFCKPKNRIKVILVGENLGF"
     gene            complement(5136..5657)
                     /locus_tag="PKH42_08565"
     CDS             complement(5136..5657)
                     /locus_tag="PKH42_08565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOA20526.1"
                     /translation="MMNPIVKFEFENGDIIKAELYPEIAPNTVRNFISLINKNFYDGK
                     IFHRVIKGFMIQGGCPNGNGMGGPGYSIKGEFNSNGFKNDLKHDEGVLSMARAMSPDS
                     AGSQFFIMHKKSPHLDGQYAAFGKVIEGMDVVNKIAATKTDRMDRPVSEVKLSKVTVD
                     TFGETYEEPEIII"
BASE COUNT         1906 a          940 c          983 g         1869 t
ORIGIN      
        1 attacttccc gttattttat ggaagcccta tatgcagaca gactgaggac ttttataaaa
       61 gaaaattaca atattgtcgc actgattgac tacagcggtg acacagcctt taaaagggct
      121 atgataagtc ctgcggtaat aatactttct aataaatgga acaaaaacat gtttttatac
      181 gtcaaaaaag acagggatgt tatcgaatca tataaatata gacaagacaa gctaaagagc
      241 tcagggtgga ttatattaaa ggatgaggat gaaaagcttt ttgagaaaat tgacagcatt
      301 tcaaacacat atatcaagga tatattaagc attaagcagg gaattattac gggctgtgat
      361 aaagctttta tagtaaccga ggaagttatc gaaaattaca aaattgagag ttttctactc
      421 aaaaaatgga ttaaaaacag caacatatca aagaaatcga taaaatataa taatctatac
      481 ctgatatatt ccgataatat acagaatgag agcgattgtc ccaatgcaat aagttatctg
      541 tccaaatata aggataaact tatgagcaga agagaatgca aaaagggatt tcgcaaatgg
      601 tatgagctgc aatggggcag aaataaatct gactacgaaa atcctaaaat aatattcccg
      661 tataaatcaa aacaaaataa tttttatttt gatgaagaag catttttttg cagtgctgat
      721 atttatttaa tgaatgaatt ttcaaaggga ttgtctttaa attatctgct ttcttattta
      781 aattctgaaa ttttcgagtt ttatttgaaa tgccagctta agaaggtagg aagaaatatt
      841 tatgaatatt atccctataa attaaataat ttgaaggtct atttacctca gggagacata
      901 gctcaaaaat tttcacgtat ggagaatatt agcattgaaa tatttaaaaa attgtttaat
      961 attaatgata aggaggtaaa tataattaag aactatatat gaagaaggtg atgacgcaat
     1021 tgaggaaatt taaaaagcta ttattattaa ttattatttt tattgtgttg tccataaagc
     1081 ctgctttagc aagaacggta ttctacgata ctttcaatca ttgggcggaa agtgacataa
     1141 attttgcctc caatactatt aatgttttta agggatatgg agatttcact ttcagaccgg
     1201 aaaatgatat aacaagagca gaatttataa ccatactatc aagaactgca tataagtata
     1261 atcttatgaa agaaatctat accggcgatt tatcatacag ggatatgtcc atgaagcatt
     1321 ggtcttatac ttttattatt tctatgaatg aatatttaaa aacaaacaaa tttactttac
     1381 aggacatctt cccgggcagt aatttttatc ctgacaaagc tatcaccaga gaggaatcag
     1441 ccgccttgat tgctgcattc tgcaaggatg caatttatga caatcctctt aggttcagcg
     1501 atgtgactac cggtaacaaa tattataatg aaatacaaag actatgcaat tcaggaatac
     1561 ttgtcggtta tgaagacaat acattcagag gcaatactaa cattaccaga gcagaagcag
     1621 cttctttaat aaagagagtt tattcggata taaaaacaag cactaacgat aattatctta
     1681 ctaaacttga atttatgcct ataaaaggcg aagagatgta ttcttatttt ggcaattatg
     1741 atttaagcac tacaaatgct aatgacaaaa aatttattaa ggctaaagac actctggaat
     1801 atgttgcatt tggcggttat atctttccgg aggattcaaa cctttatgat ttaaatgcag
     1861 ttaaaacaat ggaatccttg catgcaagcg gatatttcaa tgtagcaggc actaattatt
     1921 acatgattat gttcggcagc tattccagcg aagataaatc aaaatttgcc aatgaaattt
     1981 tgacaaatat aatagctaga aatgatttaa acgatgcgga gttaatgcag atttttacat
     2041 tggtaagcaa atatgacgta agtgaagctt tgtacatggg agcacttgaa aaatggaaca
     2101 acttaacgag caatgataat tcaaaggcta atattttatt tttcagatat gcttattata
     2161 ttaaaaacga caacaaagat attcttagag ctctggttta tgaggatttg aaaaaaaaca
     2221 acaatattgt ttctcttttg aatatcagtt ttaattcaaa cgatacaaac gcaattgatt
     2281 ttaggaatta taattttgga aattatagtt tttcattgta taaggataca accttattca
     2341 gatacttaag aaatatgact atgccattaa atactaactt aagagttgtc gaactgtcaa
     2401 atttgctgat gattgaaaaa aacccgaaac cgacagtaaa catggctgat tatgaaaatc
     2461 tgtttaccaa atattccgtt aacaaattgt atgttcttaa tttcttgggt gaaaaggaaa
     2521 gagcatttgt tgaaggaatt aacgattatg atattatcaa aacatttgaa atgtacaaga
     2581 aaaatccctc aatatttgac gacacctata cagggatttt gaaaaaagta aaagaataag
     2641 tcatagaagt ttggtgccag gcaccggtaa gtggtgcctg gcaccaagtg tctctatttc
     2701 actgttgcaa tttctatcaa cagttccaca accttttcca ttgactgaat tgatatgtat
     2761 tcgtatctac cgtgaaaatt atgacctccg gtgcatagat tcggacaagg taaacccatg
     2821 tatgacaatc tagctccgtc ggttccacct cttattggaa ctacctttgg ctcaatacct
     2881 acattttcca tagccctaat tgcattttcc acaatgtgca tgtgagggag aatcttctcc
     2941 ttcatattaa aatatgaatc ttctaatttt aactctatcg ttccttgacc gtatttttcg
     3001 tttaagtagt ctgcaatctt ttgaaatctt tcctttttta tattgaattt tgcaatatcg
     3061 tggtcgcgaa taatatattc gagcagtgtt ttttcaacat ttccggacat atcggttaag
     3121 aggctgaatc cttcgtatcc ttcggtattc tccggagttt catatacagg cagcatgttg
     3181 tgaaattcca ttccgataag tatggagttt ttcattttgt ttttggcagt tcccggatga
     3241 atattcactc cgtttattgt tacttttgca cttgcagcat tgaagttttc atactccaat
     3301 tctcctattt ctcctccgtc aacagtatat gcataatcag ccccaaattt ttcaatattg
     3361 aacagctctg ccccctgacc aacttcctca tcgggagtga atcctatttt tattgtaccg
     3421 tgtttaattt gtggattgtt gattaagtat tcagccattg acattatttc agcaattcct
     3481 gccttgtcat cggcacctag aagggttgtg ccgtctgtta caatcaaatc ctgatctata
     3541 tatttagata aatgctcaaa catttcagtt ttcattacaa tttgtttttc atcatttagc
     3601 attatgtcgc tgccgtcata attatatatt atcttaggtt ttacaccgct cccgggcata
     3661 tcgggcgatg tgtccatatg agcaataaat cctataacag gtgcatctgt attaccttta
     3721 accgttccgt aaacgtatcc gttttcatcc atgtatgcat cttcgattcc tattgatatc
     3781 atttcttcaa ccagcagtct tcctaagtcc ttttgttttt cactgcttgg aatttttttt
     3841 gcatcgtgta cggattgagt atcaatttta acataattta aaaatctttc gtgaactttc
     3901 attttgtcct cgctttatta attattttat gatattagta tatcattatg gattttatat
     3961 atcaatattc tttatgataa gaacatacct gagggtgcag cctggcattc tgagagcttt
     4021 agctcgaaga atctcatctt ataaaacctg agatccttcg cttacgctca ggatgacaat
     4081 cgcagagtat attcgtttat agtggtgtgg taatcacatg ataattctga aagctctcgc
     4141 tcgaagaatc taattgttga attgaagatt aaaataatat ataatatgga caagaatata
     4201 tgggaggttg ttatggattt tattaaagaa ggatataagg taaaaagtgc tactgttatt
     4261 aagaatcttg aaaaaagaaa tatggaaggt tactattgtg aaacagtaga agaagctgta
     4321 gaaaaagcaa tgtcaatgat taaacaggat gatacagttg gatggggcgg ttcaactact
     4381 atagaccaaa ttggaatcaa gaaacttttg gaagaaaaaa atattgctgt ctatgatagg
     4441 gataaagaaa ctgaccctgc agaaaaagta aaaatgatga aaaaggcatt gacttctgat
     4501 gtgttcctta caagtgccaa tgctattaca atggacggag aacttctaaa tattgatgca
     4561 aacggtaatc ggcttgctgc atattgctac ggtcccaaca gcgttatagt tgttgcagga
     4621 atgaacaaaa tagtaacaga tttggatatt gcactgaaaa aagcaagact tgatgcaact
     4681 gttccaaaca ccttcagaac aaactcaaaa gccccatgcc actttacggg caaatgtata
     4741 gagtgcacga tgagcgatac attgtgcggt caaattctta taacaaggtt ctgcaaacct
     4801 aaaaatagaa taaaggtaat attggttggt gaaaatttag ggttttaaga tgccctaacc
     4861 ctatttttca gagtcttttt tctgaaaaat agcggtaggt cattcgcaac gaattcccaa
     4921 tttatacgac caaagggagt aaataaattg gtgaatgaga ctgcgaggta tggggacata
     4981 ctccttagca ggaggaatgt ccccttatta cgaagtgtta tcctgaacaa agagaaggat
     5041 ctcatgagat tcttcgagct aaagctctca gaatgacagg tagcttattc atagcaatgg
     5101 ctatccacga tttattcata taaaacaaca atagtttata ttataatttc cggctcttcg
     5161 tatgtttctc cgaatgtatc aactgtaacc ttgctaagtt taacttctga aacaggtctg
     5221 tccatgcggt ctgtttttgt tgcagcaatt ttattgacca catccatccc ttcaattact
     5281 ttaccaaatg cggcatattg cccatcgaga tgaggtgatt ttttatgcat tataaaaaat
     5341 tgcgagcctg cagaatcagg agacattgcc cttgccattg acaatactcc ttcatcgtgc
     5401 tttaagtcgt ttttaaaacc atttgaatta aattcacctt ttatggaata tccgggacca
     5461 cccattccat tcccgtttgg gcatcctccc tgaatcataa accctttgat tactctgtga
     5521 aaaattttcc cgtcataaaa attcttatta attaatgaaa tgaagtttct tacagtgttt
     5581 ggtgctatct ccggatataa ttccgccttg attatatccc cgttttcaaa ctcaaatttt
     5641 actatcggat tcattattcc tcctgaaatt ttattcttta atatacttta caatatca
//
LOCUS       DAOPUE010000116         5681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14169_length_5681_cov_9.802879, whole genome shotgun sequence.
ACCESSION   DAOPUE010000116 DAOPUE010000000
VERSION     DAOPUE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14169_length_5681_cov_9.802879"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..1427
                     /locus_tag="PKH42_08570"
     CDS             207..1427
                     /locus_tag="PKH42_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOA20527.1"
                     /translation="MVSKRKYILTIVAVVLVTAFLTLTLGNVLLVEIGQKVFLSKDEY
                     SQLKKISEKYAKQESIMKIAQNEFLYEADEEVMLEGALQGTLKALGDPYTQFLTKEEF
                     ESLMQDTEGSYEGIGVYITAGDDNKIIIVSPIEDTPAEKAGLRTGDKITRINGIEYTA
                     DQIDAAVKVMKGEPGTSVTLTIQRAASDGTQDISDIVINREKIRIKTVKPAMLGDDIG
                     YIRVTTFDMQTAKDFKAAYSTLKNRGLKGLVIDLRFNPGGMISTTVEIADMFMGEGIV
                     TYTKTKAGEIEYFKSDDKMEDIPIVILINEGSASASEIMAGAMKDTNRAVLIGQKTFG
                     KGIVQKVQRFGNDGEGIKMTISEYFTPAGINIHGVGIEPDIEIQLPEDAEGYGYDFYE
                     TDTQLQKAVEVLRK"
     gene            complement(1689..3398)
                     /locus_tag="PKH42_08575"
     CDS             complement(1689..3398)
                     /locus_tag="PKH42_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOA20528.1"
                     /translation="MKRTVKIIITSFIVLIILTLNVYGLSFEASNHYELENIILEQMR
                     EYNPVFNIKYTGSLDNIEEVLKSMIDKDTYLKSNITRVDWDISGNKTASNINVRVSYI
                     MTKEERIEADKMIDEILADIIKPYMNDHEKVKAVHDYIVLNGKYDNNSLYFSDYDLLT
                     KGTSVCNGYALLTYNMLNKLNIPVNLVSGTSAGEAHIWNMVKLDDYWFHLDVTWNDPV
                     SDRDAVFYTYYMLTEKEICKDHAIDANLKIPKSTKEYYDYLVELSYNKLLVETGLDMY
                     NEENFAADESELKNLLTRKITHHPLMITVRFDKSISQDSIINAMSQLYKYDYISVINY
                     SLIDNDSKGEWNILNIFIKYKETPDNITLDFARSVYNTATEVDYNVYAQYGNKKINIT
                     KDVYIYPYDTNKINVSKGTLKFKEPGNYNLTFEYQGLRETVSITGLNSNAFEYITDKK
                     PDNYVNVKVYDQYIDFSSINQWPIIENDRTMVPLRAVFEVLNCNVKWEESSKSAVVEH
                     GSTKIIIPANSTTAYINGKANSLDVPAKIVNDRIMIPLRFVSEAIEKTVIWDDPNKTV
                     LIY"
     gene            3591..4166
                     /locus_tag="PKH42_08580"
     CDS             3591..4166
                     /locus_tag="PKH42_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOA20529.1"
                     /translation="MYKRILNEILLSEIPSEGIYKLMDSGEMNNILPELLRLKGFEQQ
                     TPYHDKDVLEHTLAVVDEIKPKLNLRLAALLHDISKPDCFTVDENGRGHFHGHHVKSA
                     LASEKILQRLGYEEELILDVTTLIRYHYIKDIAKVIKEKGIKRFVENVGAERLDDMFE
                     LIRADMTGKASTNYEVIEKLRDMCNKYEEKQ"
     gene            complement(4321..5661)
                     /locus_tag="PKH42_08585"
     CDS             complement(4321..5661)
                     /locus_tag="PKH42_08585"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOA20530.1"
                     /translation="MKKIVFILSLVLLLALSSVNAFADDVIINIDSTKVEFNEDLGFP
                     FVDENNRTQVPFRATLEKYGAVVEWDQETSTAIATKDEIVVKVPIGENYILKNDEKID
                     VDTAARIVNGRTYLPIRAVIEAFGSEVEWDGALKTVVITTVPVDAEAIYFAASDKSYE
                     WDNYDINAKINMSLNAPDGAGNVQPINMDMDMNMTIFMNPVKAKISTSMLIPGMEALG
                     QKTFMEMYMTIDEDGITQYIGMPGQTEELTWMKQTIANEMLAGFMKNNEETIEKNKEL
                     VKKYTDDVKYFGKYIEDGKTLLRLQYILSGDIYKEMFADFSEVVPEPATEEEAMALEM
                     IQNIANMDIGNLTYIIYIDEATGEMVKLEMDIAEMIASMVSGMAEITGMPPEAIEVLN
                     SLKAYMTVEFLNINSAEDFEIPEEALNAPDMLEGLQQQLETEIEIEFEAEEELQ"
BASE COUNT         1880 a          916 c          967 g         1918 t
ORIGIN      
        1 agctttgcct tgtcatcctg agcgtaagcg aaggatctca taaagggaat atcccctaaa
       61 aagatatatt aattcgcttg catttatgct ataatagcat ttgtatgtaa ggctgtatta
      121 tttttatagg ataaaataat attgagggca tataaataaa tataagcgtt taattcagct
      181 taatttttaa tagcgggagt gagtatatgg tatcgaagag gaaatatatt cttacaatag
      241 ttgcggttgt attagttaca gcctttctta ccttgacact tgggaatgtg ttattggttg
      301 aaataggaca aaaggtattt ttatcaaaag atgaatatag ccaattgaaa aaaatatctg
      361 aaaagtacgc caaacaagaa agtataatga aaatagcaca aaatgagttc ttatatgaag
      421 ctgatgaaga agtaatgctt gaaggagcgt tacagggaac gttaaaagct ttaggcgatc
      481 catatacaca gtttttgact aaggaagaat ttgaatcttt aatgcaggat acggaagggt
      541 catatgaagg cattggagtt tatataactg caggtgatga caataaaata attattgttt
      601 cacctattga ggatactccg gcagaaaagg caggactgag aacaggtgat aaaattactc
      661 gtattaacgg tatcgaatat acggcagacc aaattgatgc agctgtaaag gttatgaaag
      721 gtgagccggg tacaagtgtt acattaacta ttcaaagagc tgcttctgac ggaactcaag
      781 atatttcaga tatagttatt aacagggaga aaataagaat taaaactgtt aagcctgcaa
      841 tgcttggcga tgatattgga tatatcagag ttacaacatt tgacatgcaa acagcaaagg
      901 attttaaagc tgcatattcg actttaaaaa atagaggttt gaaagggttg gtcatagact
      961 taaggtttaa cccgggagga atgattagca ccactgttga aatagcagat atgtttatgg
     1021 gtgaaggtat agtaacctat actaagacaa aggcaggaga aatcgaatac tttaaatcag
     1081 atgataagat ggaagatatt cctattgtta tattaataaa tgaaggaagt gcaagtgctt
     1141 ctgaaattat ggccggagcc atgaaggata ccaatagggc agtcttaata gggcaaaaga
     1201 catttggaaa aggaatagta cagaaggttc aaagattcgg aaatgatggt gaaggaatta
     1261 aaatgacaat ttcagaatac ttcacaccgg caggaattaa cattcatggc gtaggaatcg
     1321 agccggatat tgagatacag ttgcctgaag atgcagaagg ctatggatat gatttttatg
     1381 aaacggatac tcagctccaa aaggcagttg aagtattaag aaaataaata atgtcatcct
     1441 gagcgtaagc gaaggatctc aaggtttaaa aaatgagatt cttcgagcta aagctctcat
     1501 aatgacggta gcttattcat agcaataaca aggttgcgaa tttatgacat attaacatag
     1561 tagaagagac agttatctgt ctcttctgtt tttaaaatat tgaattatta aggcacctat
     1621 tattaaaaga aacataatcg gataaataat atacataaat ccgaaatcaa taggcatgtt
     1681 gctacctcct aataaatcag cactgttttg tttggatcat cccaaataac tgttttttca
     1741 atagcttcgc tgacaaatct aaggggtatc attatgcggt cattaactat ttttgcagga
     1801 acgtccaaag aattagcttt gccgttaata taagccgtgg tagagtttgc aggtataatt
     1861 atttttgttg agccgtgctc aacaactgca gacttagaag attcttccca cttgacatta
     1921 cagtttaata cttcaaagac ggctcttaat ggaaccatag tcctgtcatt ttctataata
     1981 ggccactgat taattgaact gaaatctata tattggtcat aaactttaac attaacgtaa
     2041 ttgtccggtt ttttatcagt aatgtattca aatgcatttg aatttaatcc cgttatggat
     2101 actgtttcac gaagtccctg gtattcaaac gttaagttgt agtttccggg ctctttgaat
     2161 tttaaagttc cttttgaaac atttatttta tttgtatcat agggataaat atatacatcc
     2221 tttgtaatgt ttattttttt gttgccatat tgggcataga cgttatagtc tacctccgtt
     2281 gcagtattgt aaacactcct tgcaaagtct aaagttatat tgtcaggtgt ttctttatac
     2341 ttaataaata tatttaaaat attccattcg cctttactgt cattgtcaat cagactgtaa
     2401 ttaattacgg atatataatc gtatttatat aattgagaca tagcattaat tatactgtct
     2461 tgagaaattg acttgtcaaa tcttacggta atcattagag gatgatgtgt aattttacga
     2521 gtcagtaaat tttttaactc agattcgtcc gcagcaaaat tctcctcatt atacatatca
     2581 agccctgttt caaccaacaa tttgttatag gataattcta caaggtagtc ataatattcc
     2641 ttggttgatt taggtatttt caaatttgca tcaattgcgt gatctttaca aatttctttt
     2701 tccgttagca tgtagtatgt gtaaaaaact gcatctctgt ctgacaccgg gtcattccat
     2761 gttacatcta agtgaaacca gtagtcatct aatttgacca tattccaaat atgagcttct
     2821 cccgcggaag taccggatac caaattaacc ggaatattaa gcttgttaag catattgtag
     2881 gttaaaagag catatccgtt gcaaactgaa gttcctttcg tcagcaaatc ataatccgaa
     2941 aaatataaag agttgttatc atatttaccg tttaatacta tgtaatcatg aactgcttta
     3001 accttttcgt ggtcattcat gtaaggttta ataatatcag ccaagatttc gtcaatcatt
     3061 ttatctgctt caattctttc ttccttagtc ataatataag aaactcttac atttatgttg
     3121 cttgcagtct tgttgcctga tatatcccaa tccacccgcg ttatgttaga ttttaaataa
     3181 gtatccttat ctatcatact ttttaatact tcttcaatat tgtccaagga gccggtatat
     3241 ttaatattaa aaaccggatt atactccctc atttgctcaa gaataatatt ctcaagctca
     3301 taatgattgc ttgcttcaaa agacaaacca taaacattaa gagtaagaat aattaagact
     3361 ataaaagagg ttataattat tttaacagtt cgtttcatta aatcaccttc ctgactcttc
     3421 tggtatgatt atataccatt ttacataaaa taaatagtat tatattttac aattacaatc
     3481 gaatgtaata taattgtttc cgtacacact aaacaacaac gggtgagcgt tttatgccgt
     3541 attattaatt agacgtaaaa ttttagaaaa agtttcataa aaggagaaat atgtataaaa
     3601 gaattttaaa tgaaatattg ttaagtgaaa taccctcaga aggaatatat aaattaatgg
     3661 atagcggtga aatgaacaat atacttcctg aacttttaag gcttaaaggc tttgagcaac
     3721 aaactcctta tcatgataag gatgtattag agcatacatt ggctgtagta gatgaaatca
     3781 aaccgaaact taatttaaga ctggcagctc ttttgcacga tattagtaag ccggattgtt
     3841 ttacggttga tgaaaacgga agaggtcatt ttcacgggca tcatgtaaag tctgctcttg
     3901 caagtgaaaa aattcttcaa agacttggat atgaagagga attaattctg gatgtaacaa
     3961 ctttaataag ataccattat ataaaagata tagcaaaagt tataaaggaa aaaggaataa
     4021 aaagatttgt ggaaaatgtt ggggcggaaa gacttgacga tatgtttgaa cttatcagag
     4081 ccgatatgac aggcaaagct tcaacaaatt atgaagtaat tgaaaaatta agagatatgt
     4141 gcaataagta tgaagaaaaa caataggaat gatttgtaga tacatcagca acagggctta
     4201 aatttaaatt tatacccaag gatttcaaaa gctttgattt gctattaatt atactaacat
     4261 ttcataaaat ataatcaata aaaaaagtgc accgaaaagt gcacttttca tcgtttctac
     4321 ttattgtagt tcttcttcag cttcaaattc tatctcaatt tcggtttcta attgttgttg
     4381 taacccttca agcatatccg gtgcgtttaa agcttcttcc ggaatttcaa aatcttcggc
     4441 actgttgata tttaaaaatt ccactgtcat ataagccttt aagctgttta aaacttctat
     4501 tgcttcagga ggcataccgg ttatttctgc cataccggat accatagatg caatcatttc
     4561 tgctatatcc atttctaatt ttaccatttc accggttgct tcatcaatat atatgatgta
     4621 tgttaaattt ccaatatcca tatttgcaat gttttgtatc atttcaagcg ccattgcttc
     4681 ttcttcagtt gcaggctctg gaactacttc cgaaaaatca gcaaacattt ctttatatat
     4741 gtctcctgat aatatatact gaagtcttaa caatgttttt ccatcttcaa tatattttcc
     4801 gaagtattta acatcatcag tatacttttt gactaattct ttgtttttct ctattgtttc
     4861 ttcattattt ttcataaagc cggctaacat ttcatttgct attgtttgtt tcatccatgt
     4921 cagttcttct gtttgccccg gcatacctat atattgagtt ataccatctt catcaattgt
     4981 catgtacatt tccataaatg tcttttggcc aagagcttcc attccgggta tcagcattga
     5041 tgtactaatt ttagccttta caggattcat gaaaattgtc atattcatgt ccatgtccat
     5101 attgattggc tgaacgtttc ctgcaccatc gggagcatta agggacatat ttatttttgc
     5161 attaatatca taattatccc attcatagga tttatcgctt gctgcaaaat atatggcttc
     5221 tgcatctacc ggcactgttg ttattacaac agtttttaat gctccatccc attcaacttc
     5281 cgagccgaaa gcttcgatga ctgctcttat cggaaggtag gttcttccgt ttacgattct
     5341 tgcagcagta tcaacatcaa ttttttcatc atttttcagt atgtaatttt caccaatagg
     5401 aacttttaca actatctcat cctttgttgc aattgcagta ctggtttctt gatcccattc
     5461 aaccacggca ccgtactttt caagagtagc tctgaaaggt acctgtgttc tgttgttttc
     5521 atcaacaaac ggaaaaccta aatcctcatt aaattcgact ttggtggaat cgatatttat
     5581 tatgacgtca tctgcaaaag cattaactga tgataatgcc aaaagcaata ctaaacttaa
     5641 aataaatacg atttttttca taatttcctc ctaaatttat t
//
LOCUS       DAOPUE010000117         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14269_length_5651_cov_14.073445, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000117 DAOPUE010000000
VERSION     DAOPUE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14269_length_5651_cov_14.073445"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKH42_08590"
     CDS             complement(<1..415)
                     /locus_tag="PKH42_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015769590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOA20531.1"
                     /translation="MFVRKLFCISILLIGLFITGCKNVRLENPSKLPFGIFDNQENTA
                     NTIVNKEGLTIKDRYLPIEGYIRVEYEEGSFAEFLRNQKLKSYGEKVLYYDGREKSPK
                     GIYDGVFDVDIGNRDLHQCADAIMLLRAEYLYSQGL"
     gene            complement(1019..1762)
                     /gene="istB"
                     /locus_tag="PKH42_08595"
     CDS             complement(1019..1762)
                     /gene="istB"
                     /locus_tag="PKH42_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOA20532.1"
                     /translation="MANNLEQSIEMYSKRLKTPTFNQYKDVIRQLDKNQGYEDFLLSL
                     LKTEYDSRTEKSQQRKIKTAGFPYIKTIDELDTSRFEHISEPFVKELANCDFIKKRQN
                     IVMVGNPGTGKTHLSIALGIKACMQGFNVKFYTAANLANELIEAQEYKKLIRLEKQIS
                     KAELLIIDELSYLTFNRHQSELLFKVIADRAERQSVIVSTNLKFSDWLSMFENQTMVT
                     ALIDRLTFRSHVLNMNSENSYRAEHSGKG"
     gene            complement(1762..3246)
                     /gene="istA"
                     /locus_tag="PKH42_08600"
     CDS             complement(1762..3246)
                     /gene="istA"
                     /locus_tag="PKH42_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOA20533.1"
                     /translation="MKGVIIEVDIYSAIRLRYTEGESIRSISQSLGISRQTVKKYCEG
                     NTHPDVRKTYERSSAVITSDVKSFILSCFIEDETENLKKQKHTAKRIYDRLVNEQSFT
                     GSYSSVRDAVRRLRAEHAVPPQSDIPLAYDPGEAVQYDWGEATAYLDCKKTKLNLFCA
                     RLCNSCDIIVQAYQTQTQEAFLEAQQRAFDFFDGVPRRAIFDNAKVAVKEGFGLHAKP
                     QDKYLSFSAHYAFTLDFCNPAKGNEKGLVENLVGYARRNFFVPVPRVVDIKSLNEQLW
                     QSCLSYRANHKVAGRNQSVSELYAIEKEYLNAIPRFRFDTSKTAYAKVDDFSTVRYDK
                     NNYSVPTRYLRKEVTVKGYADDVCILYEGSLIATYQRSYGSSDTQYRLEHYIDLLERK
                     SRAVFNAKPVRENITKELLDWGKILPGGNKEMVKLLRLCLDYGEDKILSIKRSIPPHI
                     IPTVDMIRSYLNEPVETPVIYLNQDIEVLETDLTKYDKKFGVVK"
     gene            complement(3364..3570)
                     /locus_tag="PKH42_08605"
                     /pseudo
     CDS             complement(3364..3570)
                     /locus_tag="PKH42_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3581..4270)
                     /locus_tag="PKH42_08610"
     CDS             complement(3581..4270)
                     /locus_tag="PKH42_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20534.1"
                     /translation="MKKRRIVIIFTVILILILAYGYHRFTCNEITDTYDWTTLKNIYI
                     TLDDLLDTYEKQNNNNILDEDEIKNNLIFKEYFHQMNTYEWYLSLSPRLNSISYDIRQ
                     IADIDDGGISKQELEYLINLRDKLKIYNDKINGELEEKSIRRMFGIFRKSNVDELEAV
                     FADLLPKNNDITENDNTDSAKNVIDLTFTPIPKHTLKEGHTKEEWIKGKSIYFGKLSD
                     QIESTLDLYLV"
     gene            complement(4476..5249)
                     /locus_tag="PKH42_08615"
     CDS             complement(4476..5249)
                     /locus_tag="PKH42_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOA20535.1"
                     /translation="MRQYVVDAFTEKVFGGNPAAICIMDEWLPDDMMLNITRENNLSE
                     TAFAVKEGENYILRWFTPGGEIDLCGHATLATAYIIFRFIETEKSELCFQTLSGLLKV
                     VKKDDFYEMDFPAYELKKAGITKAMIDAIGLKPSEAYIARDLLCILESEEEVRNLKPN
                     MEKLKMLEGLLLHVTAKGKEFDCVSRSFAPKLNVNEDPVCGSGHCHIIPYWAEKSGKN
                     KLIAYQASERGGTLFCEYQGDRVKIGGKAALYSTADLHI"
     gene            complement(5266..5463)
                     /locus_tag="PKH42_08620"
     CDS             complement(5266..5463)
                     /locus_tag="PKH42_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20536.1"
                     /translation="MITKDMVILNIVEDYPSTEDIFRSYDEIAGKCTMCHNLFDTLEE
                     FAKLYDISLDDLINKLNRKIN"
     gene            complement(5504..>5651)
                     /locus_tag="PKH42_08625"
     CDS             complement(5504..>5651)
                     /locus_tag="PKH42_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA20537.1"
                     /translation="EGCGRLEWWCLDTNKPSIDFYLSLNAQPMDEWTVFRITGDTLTE
                     LAKE"
BASE COUNT         1703 a         1108 c          831 g         2009 t
ORIGIN      
        1 atagtccttg tgaataaaga tactctgccc ttaaaagcat aatagcatct gcacactgat
       61 ggaggtccct atttccaata tctacatcaa atacaccgtc ataaattcct tttggagatt
      121 tttctctgcc gtcataatat aaaacctttt cgccatagga cttaagcttt tgatttctca
      181 ggaattctgc gaagctacct tcttcatatt caactctaat gtatccttct attggtagat
      241 atctatcttt gatagtcaat ccttccttat taacgatggt atttgcagta ttttcttgat
      301 tatcaaaaat accaaaaggc aatttagaag gattttctaa tctaacattt ttacatccgg
      361 ttataaacaa tccaatcaat aaaatagaaa tacaaaatag ttttcttaca aacataacaa
      421 tccttgtctc cctatataaa ctccatagtg attgctatcg atccggatac tgctgcgtat
      481 aattcgaatt atgcatcatc gatattaatc ttgtattatg cttttattta ttcttattca
      541 aatatgttat acagactttt ataaatagca cacatgctat aaattcagta attttaaata
      601 ctacattaaa aattatcgac aaatataatg tagtcatatt caaccattcc attacttgtc
      661 ccccttatta tattttttaa gttcttataa tactacttat acgtaattat atttgaatta
      721 aaataggatg gtcttagttg tctataaaaa agttataaat cctgtcgaaa aggtgaaata
      781 tattgaatga gcttgcttcc ataccccatt ttatgtaaat gtcaaccaaa aaatttacca
      841 cctgacattg aattagttta ccacttgtga cagtgaatta gtttaccatt tatcacattt
      901 atgacagtca attagtttac cacttttaat aaaattaagt tagttcgatg acatatcgct
      961 ctgtgaatct tcaggagggc gtagcccgac tgaagattca cagagcggcg gccacaaatt
     1021 accccttacc agaatgttca gctcggtaag aattttcaga attcatgtta agtacatgag
     1081 acctgaaagt taagcggtca attaatgcag ttaccatggt ttggttctca aacattgata
     1141 gccaatccga gaacttcaag tttgttgaaa cgataacact ttgtctttcg gctctatcag
     1201 caataacctt gaacaataac tctgattggt gacgattgaa tgttaagtaa ctcaattcgt
     1261 caataatcaa taattcagct tttgaaatct gtttttctaa acgaattagt tttttgtatt
     1321 cctgagcttc aatgagttca ttggccaaat tagcagcagt atagaattta acattaaatc
     1381 cttgcatgca ggcttttatg cccagagcaa tggaaagatg tgtttttcca gtacccggat
     1441 taccaaccat cacaatgttt tggcgtttct taatgaaatc gcagttagcc agttctttga
     1501 caaatggttc tgatatatgc tcaaatcggc ttgtatcaag ctcatctatg gtttttatgt
     1561 aagggaaacc ggcagtcttg atctttcttt gctgactttt ttctgtgcga gaatcatatt
     1621 cagtttttaa aagactcagt aaaaagtctt cataaccctg atttttgtcg agctgccgta
     1681 ttacatcttt gtattgattg aaagttggtg tttttaagcg tttggaatac atttctatgg
     1741 attgttccaa attattggcc attatttaac caccccaaac tttttatcat atttcgttaa
     1801 atctgtttcc agaacttcga tgtcttgatt caggtaaata acaggtgttt ctaccggctc
     1861 attaaggtaa gaccttatca tatcaacagt tggtatgata tgcggtggta tgctgcgttt
     1921 aatggataga atcttatctt ctccataatc aagacacaga cgaagcaatt taaccatttc
     1981 cttgttgccg ccaggaagaa ttttacccca atcaagaagt tccttagtta tattttctcg
     2041 aacaggtttg gcattaaaaa cagcacggga ttttcgttcc agtaaatcta tgtaatgttc
     2101 caaacggtat tgagtatcgc tgctgccgta agaacgctga tatgttgcaa taaggctgcc
     2161 ctcataaaga atgcacacgt catctgcata accttttacc gtaacttctt tccgaagata
     2221 tctcgtagga acggagtagt tgtttttatc ataacgcaca gtagaaaaat catctacttt
     2281 tgcataagca gttttgctgg tatcaaatct aaatcttggt atagcattta aatattcttt
     2341 ttcaatggca taaagttcgg atactgattg attcctgcct gctactttat ggtttgcccg
     2401 ataacttaaa catgattgcc ataattgttc gtttaaggat ttaatatcaa caacccgtgg
     2461 gaccggaaca aaaaagtttc tcctggcata accaacaaga ttttctacta agcccttttc
     2521 gttaccttta gcaggattac aaaaatccaa agtaaaggca taatgagcac tgaaagataa
     2581 atatttgtcc tgtggtttag catgaagccc aaatccttct tttacagcga cttttgcatt
     2641 gtcgaagata gctctgcgag gcacaccgtc aaagaaatca aatgcccttt gctgtgcttc
     2701 taagaaagcc tcctgagttt gagtttggta agcttgaact attatgtcac aactgttgca
     2761 tagccttgca caaaacagat tcaatttggt ctttttacag tcaagatagg cagtagcttc
     2821 gccccaatca tattggactg cttcgccagg atcgtaagct aaagggatat cactttgagg
     2881 cggtacagca tgctcagctc tgagcctgcg gactgcgtct ctgactgatg aatatgaacc
     2941 tgtaaaactt tgttcgttta ctaatctgtc gtaaatgcgt ttagcagtat gcttttgttt
     3001 cttaagattc tcagtttcat cttcgataaa acaactgaga atgaatgatt taacatccga
     3061 tgtaattact gctgaagatc tttcatatgt tttgcgtaca tcaggatggg tgttgccttc
     3121 acaatatttt ttaacggtct gcctcgatat gccaagactt tgtgaaattg accggataga
     3181 ttcaccttct gtatatcgaa ggcgtatagc agaataaatg tccacttcta tgatcacacc
     3241 tttcatcctc cttcaaatgt atatttacat tttaaaggat tttatatgtt aggtggtaaa
     3301 ctttttcact gtcattcatc atcaaagtgg cacactttta gagtatcatt tacacatttt
     3361 atgccacttt tcgtatgtcg caatattttt tagttcgcat gtatcaaagc tttctcttgt
     3421 tattacacat atgcttcttg gttcattatc atacaaagca aacatctccc cacgctcaag
     3481 atatttgtct atcatagatt ctgactcgtc agcaagcaag agcaaatcaa gaaactgctt
     3541 tttatcctct tttacaattt taattttcac agaatacccc ctacactaaa tataaatcta
     3601 gtgttgattc tatctggtct gataatttgc cgaaataaat gctctttcct ttaatccatt
     3661 cttcttttgt gtgaccctct tttaaagtat gcttaggtat cggcgtaaaa gttaggtcta
     3721 taacattttt agcagaatca gtattatcat tttctgtaat atcattattt ttcggtagga
     3781 gatctgcaaa aacagcttct aattcatcta catttgattt gcgaaagatt ccgaacattc
     3841 ttcttataga tttttcctcc agctcaccat taattttatc attatatatt tttagtttat
     3901 cacgcaaatt tataagatac tctaattctt gtttactaat tcccccgtca tcaatatctg
     3961 ctatttgtct aatgtcataa cttatggaat taagacgggg agacaaggat aaataccatt
     4021 cgtaggtatt catctggtga aaatactcct taaaaattaa attatttttt atttcatctt
     4081 catctaaaat attattatta ttttgctttt catatgtatc taataaatca tcaagtgtaa
     4141 tataaatatt ctttaatgta gtccaatcat aagtatcagt aatctcattg catgtaaaac
     4201 gatgatatcc ataagccaat atgagaatta aaattacagt aaatataata acaatccttc
     4261 tctttttcac aacgcgctcc tttaaataac cataattaca ttgtaagaaa cagtctttta
     4321 gtttgtattc ataaagcatt acttacctgc ctgaaaaaag tatatatatc tattacagac
     4381 gtttattttt tagttttgtt ccttattata ccatatttag tggcatgtcg tcctagaaac
     4441 cttgttaaca tttcttcaag cttataatta attaactaga tatgtaaatc agctgttgaa
     4501 tataaggctg ccttccctcc tatttttact ctgtcacctt gatattcgca gaataatgtg
     4561 ccgcctcttt cagatgcctg gtaagcaatt aatttattct ttcctgattt ttctgcccag
     4621 taagggataa tatggcaatg acctgacccg cacaccggat cttcattaac attaagctta
     4681 ggtgcaaaag atctcgacac acagtcaaat tcttttcctt tagccgtaac atgtaaaagc
     4741 aatccttcca gcatttttaa cttttccata tttggtttta agtttcgtac ctcttcctct
     4801 gattctaata tacagagtaa gtctcttgct atatatgcct ctgaaggctt taatcctata
     4861 gcatctatca ttgcttttgt aattcctgcc ttttttagtt cataagccgg aaaatccatt
     4921 tcatagaaat catctttttt aacaaccttc aacaatccgc tgagagtttg aaaacaaagc
     4981 tcagattttt ctgtttcaat gaacctgaaa ataatataag ctgttgcaag agttgcatgc
     5041 ccgcataaat caatttctcc gcctggcgta aaccatctta atatataatt ctctccttct
     5101 ttaaccgcaa aggctgtttc agacaagtta ttttctcttg taatgttaag catcatatca
     5161 tctggcagcc actcatccat aatacatatt gctgcgggat tcccaccaaa cactttttct
     5221 gtaaaagcat caactacata ttgcctcatc cttacatccc ccccattaat taatttttct
     5281 attgagttta ttgattaaat catccaaact tatatcatac aattttgcaa attcttccaa
     5341 tgtatcgaat aaattatgac acatagtaca cttgcctgct atttcatcat agcttctaaa
     5401 tatatcttcc gtactgggat aatcttcaac tatatttaaa attaccatat cttttgtgat
     5461 cattaaaata acctccataa ccctccataa ccctcttttt aaattattct tttgctaatt
     5521 cagtaagggt atcaccggtt atacgaaaca cagtccattc atccattggc tgtgcattca
     5581 aggacaaata aaaatctatg cttggcttgt ttgtatccag acaccaccat tctaatcggc
     5641 cgcatccttc t
//
LOCUS       DAOPUE010000118         5645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14291_length_5645_cov_9.775492, whole genome shotgun sequence.
ACCESSION   DAOPUE010000118 DAOPUE010000000
VERSION     DAOPUE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14291_length_5645_cov_9.775492"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..629)
                     /locus_tag="PKH42_08630"
     CDS             complement(102..629)
                     /locus_tag="PKH42_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA20538.1"
                     /translation="MKNKGFSLVELLVSLGISSIIIGLIFSFFIINFKGYKSVRNDFE
                     MQFQAQYILNFMAGRIVDSNSVSFARVNTQNYSMTAVRSAGAEYPIHKISFKYGSESE
                     NYVFHIINNTIRYGKGEKEMNPTVELGNYVDEMYVSLFKDESFQDAKAVKLKLVMKKD
                     EQLHEAFQAVYMRNN"
     gene            complement(753..1292)
                     /locus_tag="PKH42_08635"
     CDS             complement(753..1292)
                     /locus_tag="PKH42_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20539.1"
                     /translation="MNNKGSTLVLLIIVIALVIVLGASVLNIAVKQYAIKKFNIDSKQ
                     AFYFSETGLNEAYVKSCALIEESIIKALQITEDYISINSFNEQAENIFVTSYKIYIGT
                     NIENRIETASNPKVKVWNDTLVFIDNALTLELKSSYIHNDIDKVTGVELVIGVPDYHD
                     VSEGSYDVRDYIKFKNWNS"
     gene            complement(1383..1769)
                     /locus_tag="PKH42_08640"
     CDS             complement(1383..1769)
                     /locus_tag="PKH42_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA20540.1"
                     /translation="MNKLFNNRGLTLAEVIMTMAILGIAICPLMNLLVLSQNINSEGE
                     KEYDVIQTAQYYMEETRAMGEIDPEIFVYNSEEMSYERMVSDVLDDLSAEIKITPFSY
                     GLHYIEVNILNDGEVINSLTGSIVID"
     gene            complement(1825..2376)
                     /locus_tag="PKH42_08645"
     CDS             complement(1825..2376)
                     /locus_tag="PKH42_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20541.1"
                     /translation="MKDKYLVFIIVLFVIAILYGLWPLYCKFNPAQNKLNTSVEAYKS
                     MMDNFRSEDSIKSKKIDYINKINKSDFRSQLSQEKIISVLYTCALKNNIRISDIKFVE
                     TDDAPDGDIDIIESKEDFVFETRCVIAEFKAGLGQLLQFIDEIKSFRDISVINLDVSI
                     WEEDIVFAFVHMKFYAVPVDMNL"
     gene            complement(2366..3802)
                     /locus_tag="PKH42_08650"
     CDS             complement(2366..3802)
                     /locus_tag="PKH42_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20542.1"
                     /translation="MFLSLEFGNNIKIVEGYKKKNIVYIKKAVTINESVDIKDILKLS
                     DSINEVLIKNNIRTKRAIFIINTESAIIRKIKLPLLNKKSEIITMIKHELGQVVSADL
                     SLYKIIYKIIETHEAEEKATALYAAYCLPLNIYEFYEKLGKKLRLKVVGIDISSNCLN
                     NILEQNIELNNHILDSKDAYAFVNTHFDKILFSVINKGNDFFMISNIERNHGNMVETD
                     HRHDRDDMVIRVEYINKCCRYYHSVNNKKVNRIYLYGYNSSDGLDKLLSSILNLEVEY
                     INSVSNISIADNISINEYFISLTALYNAKSSFLTKNEKRIQILISFAGASVILFCLLF
                     FLSKHSNSVRKQIAYLDIYIKNEKNIERNLIIEKLKSEIRLLNSAITSIETAMDLVNN
                     ENVRTENIKGVYNSLPGNTEVLSFSIDKDSLNMQCISDSMDEVLCLLRNLEGLYFVES
                     VYIPAIQSMKDMKYSYTIICNFKVNYEG"
     gene            complement(4006..4488)
                     /locus_tag="PKH42_08655"
     CDS             complement(4006..4488)
                     /locus_tag="PKH42_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOA20543.1"
                     /translation="MKNKNGFTLIELIVILALASIVMLGVMSFLITNYRSYNVINTES
                     ELQYQSQYIINYITERILEANKFEANTFYYNDDSEVTFTFDADETRDEYKNRIEYRHI
                     DGEGNEITKIPLGNYLIGLDIDISEVTESEVVIELTLQKDRSEPYTAKQTVFLRNYKK
                     "
     gene            complement(4551..>5645)
                     /locus_tag="PKH42_08660"
     CDS             complement(4551..>5645)
                     /locus_tag="PKH42_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20544.1"
                     /translation="VFTEIAGRIAKAGTDARVNGLASISSDGGYYFTILNDSKHPITA
                     KPGSATLNDNAITWNLGDITESAAELTYYIKLNLSGDIVLPPDAMLSTGEIASVVYKN
                     YRGVWVERTFPDPKLSAGGGTLNVKYYLSDEDGKPINNDGEVIPYKERVILYDNEGES
                     IGLGETISASSYAKPFKDHEVQSNTAQDENGNTLSDSILVTRATIYLNFPYYPMPKES
                     KLKNFSMYKFDGNEPAPTAGLETIEKMTYIFGLKLELGSKELELNISADNGGNIDVNS
                     FRLYDENNDIMNFYDYMFVSKVGNKCILGFKSDTDMESEKDYTIIYNVKNNNVGNYKM
                     YIDGGDLLVSEPESVSKELQIEVTKTKDIQ"
BASE COUNT         1796 a         1042 c          759 g         2048 t
ORIGIN      
        1 cgctcaggat gacaatcctt tctttatgtc attctgaggg ctttagcccg aagaatctta
       61 tgagactcct ttgctatcgt tcaggatgac aatccggaat ctcaattgtt tctcatataa
      121 accgcttgaa aagcttcatg caactgctca tcctttttca taaccagttt taattttact
      181 gcctttgcat cttgaaaact ttcatcctta aataatgaaa catacatttc atccacataa
      241 ttgccaagct caactgttgg attcatttcc ttttctccct tcccatagcg aatggtgttg
      301 tttataatat gaaatacgta attttcactt tcacttccat atttaaaaga aattttgtgt
      361 atggggtatt ctgctccggc gcttcttaca gcagtcatgg aataattttg cgtgtttact
      421 cttgcaaaac tcacagaatt agaatcaaca atccttccgg ccataaagtt taatatatat
      481 tgtgcctgga attgcatttc gaaatcattt ctcactgatt tataaccttt aaaattaatt
      541 ataaaaaaag agaaaattaa ccctataata atagaggata tccctaatga caccaaaagt
      601 tcaaccaatg aaaaaccctt atttttcata agaacccctt ttatatgata ttctttactg
      661 tgttcagaat gacgatgact tgacccttaa agccaattat taacaaaaat gacgaaattg
      721 tcatcctgag cgatagcgaa ggatctcagc ttttaactgt tccaattctt aaattttata
      781 taatctctta catcatagga accctctgat acatcatggt agtccggtac accgataacc
      841 agctcaactc ccgtaacctt atcaatgtcg ttatgaatat aagaagattt caattctaac
      901 gtaagtgcat tatcaataaa aaccaaagta tcattccata cttttacttt aggattggat
      961 gcggtttcaa ttctattttc aatatttgtt cctatataaa ttttataact tgtaacaaaa
     1021 atattttctg cttgttcatt aaatgaattt attgatatat aatcttcagt tatttgcaat
     1081 gctttaataa ttgattcttc aattaaagcg catgatttga cataggcttc attgagcccg
     1141 gtttcagaaa aataaaatgc ttgtttggaa tcaatgttga acttttttat tgcatattgt
     1201 ttaactgcga tattcaaaac ggaagctccc aaaacaatta ccagtgcaat tacaattatc
     1261 aaaagtacta aggtagaacc tttattattc atacatactc ctgttaaaca tgagattctt
     1321 cgagctaaag ctctcagaat gacacaaagg gttgtcatcc tgagcataag cgaaggatct
     1381 catcaatcta taactatact ccctgttaag ctgttgatta cttctccatc atttaaaata
     1441 ttaacttcta tatagtgcag tccgtaacta aagggggtaa ttttgatttc tgcgcttaaa
     1501 tcatccaaaa cgtccgaaac catcctttca taagacattt cttcactgtt ataaacgaaa
     1561 atttccgggt caatttcacc cattgctctt gtttcttcca tatagtattg agctgtttga
     1621 attacatcgt attctttttc accttcactg tttatattct gcgacaaaac caataaattc
     1681 atcagaggac agattgcaat tccaagtatt gccattgtca taattacctc tgccaaggtc
     1741 aatcctctgt tattaaataa tttattcaaa ataccacccc atacgaattt ttcccggacg
     1801 ctgcaaaaaa caaaatgttc tgcttcataa attcatatct acaggaactg cataaaactt
     1861 catatgaaca aatgcaaaaa caatatcttc ctcccatatt gatacatcta aattaattac
     1921 gctgatatct ctaaaacttt taatttcatc tatgaactgc aaaagttgtc ccaatccggc
     1981 tttaaactct gctattacgc acctggtttc aaaaacaaaa tcctctttcg attctataat
     2041 atcaatatct ccatccggag catcatctgt ttctacaaat ttaatatctg aaatacgaat
     2101 attgtttttc aatgcacagg tatataaaac agaaataatt ttttcctgac ttaactgaga
     2161 cctgaaatct gatttgttta ttttatttat gtaatcaatc tttttagact tgatagaatc
     2221 ctctgaccga aaattgtcca tcatggattt ataagcttca acagaagtat tcagcttgtt
     2281 ttgagccgga ttaaatttac agtacaaggg ccataagccg taaagtatag ctataacaaa
     2341 taatacaata ataaaaacta aatatttatc cttcataatt aaccttgaaa ttacaaatta
     2401 tcgtgtatga atacttcatg tccttcattg attgaattgc aggaatgtaa acactttcca
     2461 caaaatataa tccttccaaa tttctcaaca aacagagtac ttcatccatg gaatcagata
     2521 tacactgcat atttaaacta tccttatcaa ttgaaaatga taaaacctca gtattgccgg
     2581 ggagtgaatt atatactcct tttatatttt cagttctgac attttcattg ttaaccaaat
     2641 ccatagctgt ttcaatagat gttatggctg aatttaataa tcggatttct gattttagtt
     2701 tttcaattat aagatttctc tcaatatttt tttcattttt aatgtaaatg tctaaatatg
     2761 caatctgttt cctgactgag tttgaatgct ttgataaaaa gaaaagcaaa caaaaaagta
     2821 tcaccgatgc tccggcaaag cttattaaaa tttgtattct tttttcattt tttgtcagaa
     2881 atgatgattt agcattatac aatgccgtta aagatatgaa atactcattt atactgatat
     2941 tatcagcaat tgagatatta gaaacagagt ttatgtattc gacttccaga ttcagaatgg
     3001 atgacaaaag cttgtccaaa ccatcagagc tattataccc gtacaaatat attctgttta
     3061 cttttttgtt atttacggaa tgataatacc tgcagcattt gtttatatat tcaacccgga
     3121 taaccatatc atcccggtca tgcctatggt cagtttcaac catattgcca tgatttcttt
     3181 ctatatttga aatcatgaaa aaatcattac ctttatttat gacagagaac aatattttat
     3241 caaaatgagt attaacaaaa gcatacgcat ctttagagtc caaaatatga ttattcaatt
     3301 ctatattttg ttcaagtata ttgttgaggc aattagaaga tatgtctatg ccaacgactt
     3361 taagccttaa tttcttgccc agtttttcat agaattcata tatatttaaa ggaagacaat
     3421 atgctgcata caaagccgtt gccttttctt ccgcttcatg agtttcaata attttatata
     3481 taattttgta caggcttaaa tcggcggaaa caacctgacc cagctcatgc tttatcatgg
     3541 ttattatttc tgatttttta tttaataaag gcaatttgat ttttctgata atagctgatt
     3601 cggtgttaat aatgaaaatt gctcttttag ttcttatatt gttttttatc aacacttcat
     3661 ttattgaatc ggataattta aggatatcct ttatatcaac agattcattg attgtaacag
     3721 ccttttttat gtaaacaata ttttttttct tatatccttc aacaattttg atattattgc
     3781 cgaattccag ggacaaaaac ataaactcac cttatattat ttttagtaat aatataatgt
     3841 ctccctggaa gataatgtca aaatttatat tgttaattat attttagcaa tttgttttat
     3901 atttttataa tgtgtaaata aattttaata ataattggca atagtattga aagacataga
     3961 aaaacaaacc cgaatactta tctggggttt gtttttttaa gctgattatt ttttatagtt
     4021 tctcaggaat accgtctgct tagctgtgta tggctcgctt ctgtcttttt gaagagttaa
     4081 ttctataaca acttctgatt ctgttacttc cgaaatatct atatcgagtc ctattagata
     4141 attacctaag ggaattttag ttatttcatt cccttctcca tctatatgac ggtattcaat
     4201 tctattttta tattcatccc ttgtttcatc tgcatcaaaa gtaaaagtaa cctcactatc
     4261 atcattgtaa taaaaggtat tggcttcaaa tttgttagct tccaatattc tttcggttat
     4321 ataattaata atatactgag attggtattg aagctccgac tctgtattta tcacattata
     4381 acttctgtaa tttgtaataa gaaaagacat aacacctaac atcactatcg aagctaatgc
     4441 caaaataaca ataagttcaa ttaatgtaaa accattttta tttttcatat acattcctca
     4501 tattaaaatg aaattttgag cattcgccca aaatttcatt tattgagcga ttactgaata
     4561 tcttttgttt ttgttacttc aatttgcaat tctttgctca ctgactcagg ttcagatacc
     4621 aataagtctc caccgtcaat atacattttg taattaccga cattattatt tttcacattg
     4681 taaattattg tgtaatcttt ttcggactcc atatcggtat cacttttaaa acctaaaata
     4741 cacttatttc caacctttga aacgaacata taatcgtaaa aattcattat atcattattt
     4801 tcatcataaa gcctaaaact gttgacatca atatttcctc cattgtcagc agaaatattt
     4861 aattctaact ccttgcttcc aagttcaagc tttaacccga agatatatgt cattttttca
     4921 atcgtttcca agccggctgt cggtgcaggt tcattgccgt caaatttata cattgaaaag
     4981 tttttcagct tactttcctt cggcatcgga taataaggga aattcaaata aattgttgct
     5041 cttgtcacaa gtatgctgtc agatagagta tttccatttt cgtcttgggc agtattagac
     5101 tgaacttcgt ggtccttaaa tggttttgca tatgaagatg cagatatcgt ttctcctaaa
     5161 ccaattgatt ctccttcgtt gtcatatagg attacacgtt ccttgtaggg gattacctct
     5221 ccatcattat ttataggttt accatcctca tcagaaaggt agtatttaac atttaaggta
     5281 ccaccgcctg cagacaattt cgggtcaggg aatgttcttt caacccaaac acctctgtaa
     5341 tttttataca caaccgatgc aatctcgcct gtgctaagca ttgcatcagg tggcaataca
     5401 atgtcgcctg ataaattaag cttaatataa tatgtcagct ctgccgcaga ttcggttata
     5461 tcacctaaat tccatgttat tgcattgtcg tttaaggttg cagagcccgg cttggcggtt
     5521 ataggatgct ttgagtcgtt caatatagta aaataatatc ctccgtcact gctgatgctt
     5581 gccagcccat ttacacgggc atcagtccct gctttggcaa tacgccctgc aatttcggta
     5641 aacac
//
LOCUS       DAOPUE010000119         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14568_length_5561_cov_8.857792, whole genome shotgun sequence.
ACCESSION   DAOPUE010000119 DAOPUE010000000
VERSION     DAOPUE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14568_length_5561_cov_8.857792"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PKH42_08665"
     CDS             complement(<1..266)
                     /locus_tag="PKH42_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20545.1"
                     /translation="MFSKTSIAKIHKNGKINKIVTAIKFTFYGVFMMKTKNILYFLKF
                     NITIILFLSALYFENAHQPRLIVLMVIFVLFLANNIAKYRLKAQ"
     gene            complement(331..1089)
                     /locus_tag="PKH42_08670"
     CDS             complement(331..1089)
                     /locus_tag="PKH42_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOA20546.1"
                     /translation="MEGIQKVDLTKIKPYGDTLNDGMVQMSFTLPVPYGDEANEAAKQ
                     LVSKMGFENPAVVFSKDLGVGYTYFIMYGKCTHTVDYTSIQVPKADIEFMQREEVEEY
                     IKENIKRDVVIVGACTGTDAHTVGIDAIMNMKGFDGNYGLERYKGIEAINMGSQVPNE
                     ELIARAIEVKADAILVSQVVTQKNIHISNLTQLVEMVEAEGLRDKVILVCGGPRISHE
                     LAQELGYDAGFGTGSYANHVASFVVKQIVQRNLI"
     gene            complement(1089..2645)
                     /locus_tag="PKH42_08675"
     CDS             complement(1089..2645)
                     /locus_tag="PKH42_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOA20547.1"
                     /translation="MESKLNLDPRLIDSARIAARNIADDVQKFIDKNTTVTVERTVAR
                     LMGVDGVDEIEKPLPNVLIDSIKEGGGLSRGAAFWIGNAMVNTGKTPQEIAEKVSSGE
                     IEITRLPIADENKIMEAVYETAKQMVKKIKNSRAKRDEYLKKLGEGRKPYLYVIVATG
                     NIYEDVLQAQAAAKQGADVIAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRK
                     ALDEVGEEVGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRDINMQRTL
                     VDQYFSRIINGFAGIIINTGEDNYLTTADAYEEAHTVLASQFINEQFAIKAGLPEEQM
                     GLGHAFEMNPMLENGFLYELAQAQMAREIFPKAPLKYMPPTKYMTGNIFRGHLQDAMF
                     NLISIWTHQGIQLLGMPTEAIHTPHLQDRMLSIENAQYIFNNARDIGNEIEYKKDGII
                     QKRAQEVLAKAEELLKDIEKEGIFNTIEKGKFGGVKRSFTGGKGLEGVVEKDEKYFNP
                     FIDLMLGGAK"
     gene            complement(2722..4320)
                     /locus_tag="PKH42_08680"
     CDS             complement(2722..4320)
                     /locus_tag="PKH42_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOA20548.1"
                     /translation="MNSFMTENTIKNVNFDYIFNEVKPITEYGIRTKQEAKPFIKGQE
                     ADLKAEFNKIGAFIESRQRRDIIDVLKHIKNILETIQRAKSNQVLDEVELFEIKNFLI
                     LVEKMDKILRGISNNDLKLLQLTPLPQLYEKLDPAHEKLNTFYIYDEYSQKLKKIRNE
                     KRQIEKNISLLKKRIREEIQEKHNIKLNLKDEVTISKSQKDKINELNKEVNLRLFGEN
                     YLNIIYKIKNSDEIDELEKRYEELTFEEDDEEYKIRQKLSVSIKEFSHILQKNTEIIG
                     EIDYLIAKANYAQKTCSVEPEIINELQITIKGGRNLKLEKILKDKHIEYVPIDLNLNK
                     NVSCITGANMGGKTVTLRMIGQIAAMASFGMFVPCEYARLCLFERIFISAGDNQSIEK
                     GLSTFGAEIVNLKEAIKSSDLRCLILIDELAGGTNPKEGYAITKAVVNYLKKKDCITV
                     LTTHYDNIANDEDIKNLQVKGLSLPDETDFSKQDNIDSIQKYMDYRLIEVNYSGDIPK
                     DALRIAKMAGIDEEIIKDAENYLK"
     gene            complement(4317..5345)
                     /locus_tag="PKH42_08685"
     CDS             complement(4317..5345)
                     /locus_tag="PKH42_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20549.1"
                     /translation="MRLIETIKQNNYETVSIIGLAKNAGKTVTLNYLIEEAINLNIKT
                     GITSTGRDGENTDLITKTQKPAISVTEGMYVATAKKTLMLSNAKAEILETTGISTAMG
                     EVIIIRVRQKGNIQIAGPVSAKDMKYVARRLKYYGAEVVFIDGAIDRKAVSSPAITDA
                     CVIATGAVLSRDMKKVLEKTAHAVECYSLTGTDEHVKNIVRKINKTCIISEDGKTLVP
                     DIKTSITSGKKISEMISEKTTHVFVKGAVTSALLKDLWENKYLRGIKLIIEDGTKIFT
                     DINMWNEMRRKGLKVEAINTINVLAVTLNPVSPEGYFFDSEVLKENMKKVLTGIKIVD
                     VVSGGEED"
BASE COUNT         1429 a         1177 c          837 g         2118 t
ORIGIN      
        1 ttttgagctt ttaaacggta tttggctata ttattggcca aaaaaagcac aaagattacc
       61 ataagcacta tcagcctcgg ttgatgtgca ttttcaaaat aaagtgccga taaaaataag
      121 atgatggtta tattaaattt aagaaaatat aaaatatttt tggttttcat cataaaaact
      181 ccgtaaaacg tgaactttat agctgttact attttattta ttttaccatt tttatgtatt
      241 tttgcaatgg atgttttaga aaacataaaa aaaggtgcca aaaaaaggtg ccaggcacct
      301 tttcaggtgc ctggcacctc aatataactc ttagattaaa tttctttgaa caatttgttt
      361 taccacaaat gatgcaacgt ggttagcata gcttcctgtc ccaaaacctg catcatatcc
      421 taattcttgt gcaagctcgt gacttatacg agggcctccg catactaaaa tcaccttatc
      481 cctgagtcct tctgcttcca ccatttcaac taattgtgtc aggttggaaa tatgaatatt
      541 tttttgtgta actacttggg ataccaatat tgcatctgct tttacttcaa tggcccttgc
      601 aataagttct tcgtttggta cctggctgcc catgttgatt gcttcaatgc ccttgtatct
      661 ttcaagtccg taattaccgt caaagccttt catattcatt atggcatcta ttccgacggt
      721 gtgagcatca gtacctgtgc atgctcctac aatcactacg tcgcgcttga tattttcctt
      781 tatatattct tcaacttctt ctctttgcat aaattcaata tctgcctttg gaacttgtat
      841 cgaagtatag tcaacggtat gagtgcattt tccatacatt atgaaatatg tgtatcctac
      901 tcccaaatct tttgaaaaaa caaccgccgg attttcaaat cccattttag ataccaattg
      961 tttcgctgct tcatttgctt catcgccgta aggaacgggt aatgtaaaac tcatttgaac
     1021 cattccatca tttaatgtat ctccatatgg tttgattttc gttaaatcta ctttctggat
     1081 accttccatt attttgcacc ccccaacatt aaatcaataa atgggttaaa gtatttttcg
     1141 tctttttcaa caacgccttc caatcctttg ccgccggtaa agcttcgttt tacaccaccg
     1201 aatttacctt tttcaattgt attgaatatt ccttcttttt caatatcctt taacagctct
     1261 tcagctttag ccagcacctc ctgtgctctt ttctgaatta ttccgtcttt tttgtattca
     1321 atttcattgc ctatatcacg agcattattg aaaatatact gtgcattttc tattgaaagc
     1381 attctgtcct gaagatgcgg tgtatgaatt gcttccgtag gcatccccaa taattgaatt
     1441 ccctgatgag tccatatgga aattaaatta aacattgcat cctgtaaatg tcctctgaat
     1501 atatttcctg tcatatattt tgttggaggc atatatttaa gcggtgcttt ggggaaaatt
     1561 tctctagcca tctgagcttg agccaattcg tacaagaagc cattttctaa cattgggttc
     1621 atttcaaaag cgtggccgag acccatttgt tcctctggaa gtcctgcttt tattgcaaac
     1681 tgctcgttaa taaactgaga tgccaatacg gtgtgagctt cttcataagc atctgccgta
     1741 gtcagatagt tgtcttctcc cgtatttatg ataatacctg caaagccgtt gattattctt
     1801 gaaaaatatt ggtcaaccaa tgttctttgc atgttaatgt ctctgaaaag aattccgtaa
     1861 agagcatcgt taagcattac atcaagcctt tctattgctc ccattgctgc tatttcaggc
     1921 atgcaaagac ctgagcagta gttgcaaaga cgaatatatc ttcctacttc ttctccaacc
     1981 tcatcaagag ctttacgcat tatcctgaag ttttcctgag ttgcataagt tccgccgaag
     2041 ccctcggttg taggtccgta aggaacatag tcaagcaagc tttgagcagt tgttcttata
     2101 acggcaatta catcggcacc ttgctttgca gccgcctgcg cctgtagaac atcttcataa
     2161 atattgcctg ttgcaactat tacatacaaa tatggttttc ttccttcacc gagttttttt
     2221 aagtattcat ccctttttgc tctgctgttt ttaatctttt taaccatttg ctttgcagtt
     2281 tcataaactg cttccataat tttgttttca tcggcaattg gcagtcttgt tatttcaatt
     2341 tctcccgagc ttactttttc tgctatttcc tgaggggttt ttccggtatt aaccatggca
     2401 tttcctatcc aaaacgctgc tcctctggat aatccgccgc cttccttaat agaatctatc
     2461 aatacattgg gaaggggttt ttctatttca tcaacgccat ctacccccat gagacgtgca
     2521 acagtccgtt caactgtaac agttgtattt ttgtcaataa atttttgtac atcgtctgca
     2581 atatttcttg ctgcaatacg tgcactgtca attaatcggg gatcgagatt taatttactt
     2641 tccatacatc ctcctcttac ggggacattc cttcatggtc cggcctcaac ggcttactct
     2701 ccatgaagag tgtcgccctt cctattttaa ataattctct gcatctttta ttatttcttc
     2761 gtctattcct gccatttttg ctatccttaa ggcatctttc ggaatatctc cggaataatt
     2821 cacttctata agcctgtaat ccatatattt ttgaatgcta tcgatgttgt cttgtttgct
     2881 aaaatcagtc tcatcaggaa gactaagccc ttttacctgc aaatttttta tgtcttcatc
     2941 atttgcaata ttgtcataat gagttgtaag tactgttatg caatcctttt ttttaagata
     3001 atttactact gcctttgtaa ttgcataacc ttcctttgga ttagtacctc ccgccagctc
     3061 atcaatcaaa atgagacatc ttaagtcaga gctttttatt gcttctttta gatttactat
     3121 ttcagctcca aaggtagaaa gacctttttc aattgactga ttgtcaccgg cagaaataaa
     3181 tatgcgctca aaaagacata atcttgcata ttcacagggt acaaacatac caaagctagc
     3241 cattgctgca atttggccta tcatcctaag ggttacggtt ttccccccca tatttgcccc
     3301 tgttatgcaa gacacatttt tattaagatt taaatcaatg ggaacatatt caatatgttt
     3361 atctttcaat attttttcaa gttttaggtt tctgcctccc ttaattgtga tttgcagctc
     3421 atttattatt tccggctcta cggagcaggt tttttgtgca taatttgctt ttgcaataag
     3481 atagtcgatt tcaccgataa tttctgtgtt cttttgcaat atatggctaa attcttttat
     3541 tgacaccgag agtttttgtc gaattttgta ttcttcgtca tcttcttcaa aagtgagttc
     3601 ttcgtacctt ttctccagct catcaatttc atcggaattt ttaattttat aaataatatt
     3661 taaataattt tctccgaata atcttaaatt gacttcctta ttcagctcat taatcttatc
     3721 tttttgtgac ttgcttattg ttacttcatc ttttaaattt aatttaatat tatgcttttc
     3781 ttgtatttct tctctgattc ttttttttaa taaactgata tttttctcta tctgtcgttt
     3841 ttcatttcga atttttttta atttttgcga atattcgtcg taaatataaa atgtgtttaa
     3901 cttttcatga gccgggtcaa gtttttcata tagttgtggc aggggcgtca attgtaatag
     3961 ctttaaatca ttattggaaa tgcctcttaa aattttatcc atcttttcga ctaaaatcaa
     4021 aaagtttttt atttcaaaca attctacttc atcaagaacc tgattgcttt ttgccctttg
     4081 gatagtttcg agtatatttt taatgtgctt caaaacatcg attatgtccc gcctttgtct
     4141 gctttcaata aatgccccga ttttattgaa ctccgcctta aggtctgcct cctgaccttt
     4201 tataaaaggc ttagcttcct gttttgtcct tatcccgtat tctgtaatcg gttttacttc
     4261 attgaaaata taatcgaaat ttacattctt tattgtattt tccgtcataa agctattcaa
     4321 tcttcttcac cgcccgacac cacatcaact atttttattc ccgtcaggac ttttttcata
     4381 ttttctttta aaacttcact gtcaaagaaa tatccttccg gagataccgg atttaaagtt
     4441 accgccagaa catttattgt atttatggct tcaactttaa gtcctttcct tctcatttca
     4501 ttccacatat ttatgtccgt aaatattttt gttccgtcct ctataattaa tttaatgcct
     4561 cttaaatatt tgttttccca taaatctttc aacaaggcag atgtaaccgc acctttaaca
     4621 aacacgtgag ttgttttttc actaatcatt tcactgattt ttttgccgct cgtaatactt
     4681 gttttaatat cgggaacaag agttttaccg tcttctgata tgatgcatgt tttattgatt
     4741 ttccgcacta tattcttaac atgctcatcc gtacctgtaa gcgagtagca ttccacagca
     4801 tgggcagtct tttctaaaac ctttttcata tctctgctaa gtacagcacc tgttgcaata
     4861 acacatgcat ctgtaatagc cggagatgaa acagctttcc tgtctattgc tccatctatg
     4921 aataccactt ctgctccgta atatttaagg cgcctcgcaa catatttcat atcttttgcg
     4981 ctgacaggtc cggcaatctg tatattgcct ttttgtctca cccttataat tattacttcg
     5041 cccatggcag ttgatatccc cgtagtttcc aatatttcag ccttagcgtt tgaaagcatc
     5101 aatgtttttt tcgcagtggc aacatacatt ccttcagtta ccgaaattgc aggcttttgt
     5161 gtttttgtta ttaaatctgt attttcaccg tcgcgccctg ttgaagtaat acctgtttta
     5221 atgtttaaat ttattgcttc ttctatgaga taattaagtg tcacggtctt tcctgcattt
     5281 tttgcaagac ctattatgga tacagtttca taattatttt gttttatggt ttcaattaat
     5341 ctcatagaga acctcttata gtcagttcat tgttatcatt atagcgcagt cccgctgcac
     5401 aatttcgtct gctcctttcg tcgcataaaa ttggcgccgg ttagggcatc tcgcagtctc
     5461 attcaccaat gtatttgctc cctttggtcg cataaaattg gcgccggtta gggcatctcg
     5521 cagtctcatt caccaatgta tttgctccct ttggtcgcat a
//
LOCUS       DAOPUE010000120         5552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14600_length_5552_cov_7.268874, whole genome shotgun sequence.
ACCESSION   DAOPUE010000120 DAOPUE010000000
VERSION     DAOPUE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14600_length_5552_cov_7.268874"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKH42_08690"
     CDS             <1..498
                     /locus_tag="PKH42_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOA20550.1"
                     /translation="TFDIDANGIVNVSAKDMGTGKEQKITITASTKMSDADIEKKIKE
                     AEQFAEEDRKKKEAIEIKNNADTLVYQTEKALKDLEGKISAEEKAKAQAAADELKKAI
                     ESNDTDQMKQKTEELTKVFNDLAQKLYAQTGAQGGAEETNPNQGSAPDEDIVDADFEE
                     VDDDK"
     gene            716..1867
                     /gene="dnaJ"
                     /locus_tag="PKH42_08695"
     CDS             716..1867
                     /gene="dnaJ"
                     /locus_tag="PKH42_08695"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOA20551.1"
                     /translation="MQGGERVSKRDYYEILGISKDASEQEIKSAFRKLAKKYHPDLNP
                     DDKEAEAKFKEVNEAYEVLSDPERKAKYDQFGHAAFDQANGFGGAGFNDFSDIFGDIF
                     GDFFGGGFSGSTRTQRTGPKAGSDLKIKLDIDFEEAAFGTKKEIKINRIEKCSVCDGT
                     GAKKGTSKKTCPTCQGSGSIRTVQRTPFGQFASTKICSTCSGMGEVVEEPCTSCSGSG
                     KERKSRKLSINIPAGVDTGSVIPLRGEGNHGERGGPAGDLYVYINVRPHEIFERDGND
                     VWCEIPISFAKAALGGSIEVPTIEGKVKYDVPEGTQTGTVFRLKNKGIKNLRGAGKGD
                     QYVRVKIEVPKKLTEKQRNILEQFANELGEKADNDKKGFFGKMKDAFRD"
     regulatory      2022..2105
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2217..3650
                     /locus_tag="PKH42_08700"
     CDS             2217..3650
                     /locus_tag="PKH42_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20552.1"
                     /translation="MSNINDMPAEKATNNVETDMAELKKLQQRLNHESIKYQFRTKLV
                     GGLHEEDVTRYIEDLENKLKKFEQDNKKISDDLYSLKTKLNTELEHKNNMQIELDEAK
                     QNLITAIVEFKQKQMDYKSMNEKYSAENIQLKNEIQQITGRLKELEKLSTETNYDKDC
                     LIDLENENKALKTKIAEMTEEFMQYNGIKNENKQLLEELEANKKLLIQSRAECEQLRE
                     ISSKLENENNQIKIKISELSDENQHIDILKNENKQMAEEIAAYEELLNQTSIELDQME
                     VNTNKLKNENILLKERITELEKSILEKDYRINEINNVCEKLEEELKIEKSNSEKLNSD
                     SVILRQKISSLQQTINEKLKELEEQKRISEDAVLQLNMEKAEAFNYKINGFKEEFSSI
                     YEKMEKLEYEAKQSAKLNGILKQQLSAQHSRAEKAEEDLAKIVKLLSEVGDKFYNKRD
                     ILGDEFIQLIEKQISRQPEKNGKIINL"
     gene            3694..5040
                     /locus_tag="PKH42_08705"
     CDS             3694..5040
                     /locus_tag="PKH42_08705"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOA20553.1"
                     /translation="MINTIGITKLQSYEIRNSKLDNVAKVTVILALSVIPAVFVPLGG
                     TTDHFYLPKVAAMIILALSFLAVLAINKIRFKDIVQKDRINISLFIYFILLAASVYAA
                     ENKVFAIIGVPGRWEGLVTITLYMFLFITARLYLVPDEGLFKIILVTAIIVSIYGILQ
                     TMNFDPFPRDVLRENWSKRAFSTMGNPNFLGSYIVLIIPTSIYFYIIKKNITGLTAYA
                     ILFYCLLSTGTRGAWLGTIASIMAFAAIHYMYFRYSKGEFTRYIILLVITILLLALYN
                     FNTEGAFIDRFLSIARDANEFLTKGDRADYSGANRGFIWKRVAELIKKRPLAGYGIEN
                     LGEAFKKYYTKDMIELWNEVRYLDKAHNEYLHIAVTSGIPSLLVYLTFISQIILKGLF
                     RLKNCKTALLILSSVIGYMTAAFFNISVVSVAYVYWIFLGLLAGSNGNCYKFNLRA"
     gene            5090..>5552
                     /locus_tag="PKH42_08710"
     CDS             5090..>5552
                     /locus_tag="PKH42_08710"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOA20554.1"
                     /translation="MLYKLIVFLTLGLFTGYIIPKLSLKLIEYKEKTKNIDVNKAQPS
                     GSRINILIILFNGAAWCFTAYTQESLFTAVLICIQISLGLIFAYIDIKIRIIPNELIL
                     ILLTLGITYQAFSYGLEGILGSVICMIFIMIVFTALATFMGLGKVGAGDVKL"
BASE COUNT         2156 a          824 c         1118 g         1454 t
ORIGIN      
        1 acatttgata tagatgctaa cggtatcgta aatgtaagcg caaaggatat gggcacaggc
       61 aaggagcaaa aaataacaat aacagcttca actaaaatgt ccgatgccga tatagaaaag
      121 aaaattaaag aagcagagca atttgcggaa gaagacagaa agaaaaaaga agcaatcgaa
      181 attaaaaaca atgcggacac attggtatat caaacagaaa aagcactgaa agacttagaa
      241 ggcaaaattt ctgcagagga aaaagctaaa gcacaagctg cggcagatga acttaaaaaa
      301 gcaattgaaa gcaatgatac agaccaaatg aaacaaaaaa cagaagagct tacaaaggta
      361 tttaatgacc ttgctcaaaa gctttatgca caaacaggtg cacagggcgg agctgaagaa
      421 actaatccta accaggggtc tgcaccggat gaagatatag ttgatgcaga tttcgaagaa
      481 gtcgacgacg acaaataatt tcattcttag ggcgaagccc gaagaatctc aagagatcct
      541 tcgctgtcgc tcaggatgac ggctatgtca ttctgagggc tttagcccga agaatctcaa
      601 gagatccttc gctgtcgctc aggatgacgg gtatgtcatt ctgagggctt tagcccgaag
      661 aatctcatgt ttaacaacca accaagctaa atcctagtag ggtttagctt gattaatgca
      721 aggtggtgaa agagtgtcca aaagagacta ctatgaaatt cttggaataa gtaaggatgc
      781 cagtgaacaa gaaataaaat cggcattcag aaaattggca aaaaaatacc atcctgattt
      841 gaacccggat gataaagaag cagaagcaaa attcaaagaa gtaaacgaag cctatgaagt
      901 tctgtcagac cctgaaagga aagcaaagta tgaccaattc ggccatgcgg cattcgacca
      961 ggctaacggc tttggaggag caggattcaa tgacttcagc gatatattcg gtgatatatt
     1021 cggtgacttc tttggaggag gtttcagcgg aagtacaaga actcagagaa ccggacctaa
     1081 agcaggctcc gatttgaaaa taaaattgga tatagacttt gaagaagcag cattcggaac
     1141 gaaaaaggaa attaaaataa atagaataga aaaatgctcc gtctgtgacg gaacaggagc
     1201 taaaaaaggc accagcaaaa aaacatgtcc tacctgccaa ggctcaggca gcataagaac
     1261 agtgcaaaga acaccattcg gacaatttgc aagcacaaaa atatgctcaa catgcagcgg
     1321 tatgggagaa gtggtagagg agccgtgtac ttcatgcagc ggctcgggaa aggaaagaaa
     1381 atcaaggaag ctttcaatca acattcctgc aggagttgac acaggctcgg taatcccctt
     1441 aaggggtgaa ggaaaccacg gtgagcgcgg aggtcctgcc ggagatttgt atgtatatat
     1501 taatgtaaga cctcatgaaa tatttgaaag agacggaaac gatgtgtggt gtgaaatacc
     1561 aatatcattt gcaaaagcag ccttgggagg ctctattgaa gttccgacaa tcgaaggcaa
     1621 ggtaaaatat gatgtacctg aaggaacaca gacaggaaca gttttcagac taaaaaataa
     1681 aggtattaaa aacctaagag gcgccggcaa aggcgaccag tatgtgagag tcaaaataga
     1741 agttcctaaa aagcttacgg agaaacaaag aaatatctta gagcaatttg caaacgaatt
     1801 gggagaaaaa gcagacaatg acaaaaaagg ctttttcgga aaaatgaagg atgcttttag
     1861 agattaataa ttctttcgac atatttcgat aatatataaa gcaataaaaa aaatcatcat
     1921 aatttcttaa aaaataatga caaattatca aaaaaaactc ttgcattatt ttgtcatata
     1981 tagtagaatg tataaacaga tagaaaaatt aataaattat cctacgataa tagcaaactt
     2041 gtcgaaaggc aaggacgcaa agccatgggt ctaatgtgaa atacgatgat tgccaggttg
     2101 ccgaacagtg atgaattaat gcattaaatg tattgatgta tttttggtct ttaactgtac
     2161 gccttggcgt acagttttta ttttgaaatc aaataacttg aggaaggggg gataaaatgt
     2221 cgaatattaa tgatatgcct gcagaaaagg caactaataa tgtggaaaca gatatggcag
     2281 agcttaagaa gcttcagcaa agactcaatc atgagtcaat taaatatcag tttagaacaa
     2341 agttagttgg cggtttgcat gaagaagatg ttaccaggta tatagaagac ttggaaaata
     2401 aattaaaaaa atttgagcag gataacaaaa aaatatcaga cgatctttat tctctaaaaa
     2461 ccaaactcaa cactgaatta gaacataaga acaatatgca gatagaatta gatgaagcaa
     2521 aacaaaattt aattactgcc atagttgaat tcaagcaaaa acaaatggac tacaagtcta
     2581 tgaatgaaaa atacagtgcc gagaacattc aattgaagaa tgaaattcaa caaatcacag
     2641 gcaggctaaa ggaacttgaa aaactatcaa ctgaaactaa ctatgataaa gactgcttaa
     2701 tagatttgga aaatgaaaac aaggccttaa aaaccaaaat agctgaaatg acagaagaat
     2761 tcatgcaata taacgggatt aaaaatgaaa acaaacaact tttagaggaa cttgaagcca
     2821 ataaaaaact tttgattcaa tcaagagcag aatgcgaaca attaagagaa atttcatcaa
     2881 aattagaaaa tgaaaataat caaataaaaa ttaaaatatc tgaattatca gatgaaaatc
     2941 aacatattga tattcttaaa aatgaaaaca agcaaatggc tgaagagatt gcggcctatg
     3001 aagaactatt aaatcagaca agcattgaat tagaccaaat ggaggtaaat acaaataaac
     3061 tcaagaatga aaatatattg ctgaaagaaa gaataactga actggaaaag agtattcttg
     3121 aaaaggatta tagaattaat gaaattaata atgtttgcga aaaactggaa gaagaactta
     3181 agatagaaaa atcaaacagc gaaaaattaa attcggattc agtaatatta agacaaaaga
     3241 taagcagtct gcagcaaaca ataaatgaaa aattgaaaga attagaagaa caaaaaagaa
     3301 tcagtgagga tgcggtatta cagcttaaca tggaaaaggc agaagcattt aactataaga
     3361 taaacgggtt caaagaagaa ttcagcagta tttatgaaaa aatggaaaaa ttggagtatg
     3421 aagcaaaaca aagtgcaaag ctaaacggaa tacttaaaca gcagttatct gctcaacaca
     3481 gcagggcaga aaaagcagaa gaagacttag ctaaaattgt aaagctgctc tctgaggtgg
     3541 gagataaatt ttacaataaa cgagatattc ttggagatga gtttatacag ctgattgaaa
     3601 aacaaatatc aagacagcct gaaaaaaacg gtaagataat aaacttataa tttttataac
     3661 catagctcct tactatatat taaggaagta attatgatta atactattgg tataacaaaa
     3721 ctacagtcct atgaaataag aaacagcaaa ttggataatg ttgcaaaggt tacggtaata
     3781 cttgcattat cagttattcc ggcagtattt gttccattgg gaggtactac agaccatttt
     3841 tatttaccaa aagtcgcagc aatgattatc cttgcattaa gctttttagc ggtgctggca
     3901 ataaacaaaa tccgttttaa agatatagta cagaaagaca gaattaatat tagtttattt
     3961 atatacttca tattactggc tgcttctgta tatgcagctg aaaataaagt gtttgcaata
     4021 atcggagtgc ccggcagatg ggaaggtttg gttacgataa ccttatatat gtttctcttt
     4081 ataacagcaa ggctctactt ggttcccgat gaagggctgt ttaagataat actggttacc
     4141 gccataattg tttcaattta cggtatttta caaactatga attttgaccc ctttccaagg
     4201 gatgtcctcc gtgaaaactg gagcaaaaga gctttttcaa caatgggtaa tcctaacttt
     4261 ttaggttcat atatagtttt aataatacca acatcaatct atttttacat aatcaaaaaa
     4321 aacataacag gtttgacggc atatgcaatt ttgttctatt gcttattaag taccggcaca
     4381 aggggggctt ggttaggtac aattgcatca ataatggctt ttgcagcaat acattacatg
     4441 tattttagat atagcaaggg ggaatttacc aggtatatta ttctgttagt tataacaata
     4501 ttgctcttag ccttatataa ttttaatacg gaaggtgcat tcatagatag atttttatct
     4561 attgcaaggg atgcaaatga atttcttact aagggtgata gggccgatta ctccggtgcc
     4621 aacagaggat tcatttggaa acgagttgca gaattaataa aaaaacgacc tctggccgga
     4681 tacggtatag aaaatttagg agaagcattt aagaaatact ataccaaaga tatgattgag
     4741 ctttggaatg aggtgcgata tctcgataag gcacataatg aatatttaca cattgcagtt
     4801 actagcggca ttccatcttt attggtttac ttgacattta tttctcagat aattttaaag
     4861 ggtttgttta gattaaagaa ttgtaagaca gcactcttaa tattatcaag tgttataggt
     4921 tatatgacag cagctttctt taacataagt gtggtatcag tggcatatgt gtactggata
     4981 tttttaggat tattagccgg cagcaatggt aattgttata aattcaactt aagggcatag
     5041 acctcgttgc agtagtgcgg ctaacaacaa tacattaaag aggtatgaaa tgttatacaa
     5101 attaattgtt ttcttaactt taggtttatt tacgggatat ataattccca agctgtcgtt
     5161 aaagcttatt gagtataaag agaaaaccaa gaacatagac gttaacaaag ctcaaccgtc
     5221 aggcagccga ataaatatat tgataatcct gtttaacggg gcagcatggt gttttacggc
     5281 ttatacacaa gaaagcttat ttactgcagt actcatatgt atacaaatat cattaggatt
     5341 aatatttgca tacatcgaca taaaaataag aataattcct aatgagttga ttttaatatt
     5401 attaacctta ggcatcacat atcaggcatt tagttatggg ttggaaggaa tcttaggctc
     5461 agtaatttgt atgattttta ttatgattgt gtttactgca ctggcaacct ttatgggatt
     5521 aggcaaggtt ggagcaggtg atgtgaaatt ag
//
LOCUS       DAOPUE010000121         5545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_14628_length_5545_cov_15.208743, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000121 DAOPUE010000000
VERSION     DAOPUE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14628_length_5545_cov_15.208743"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PKH42_08715"
     CDS             <1..1321
                     /locus_tag="PKH42_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOA20555.1"
                     /translation="ASQVLKEQYWKRTMGTVRTVLEKIMRLLECYDLKPEMAHKEVGG
                     ITNKIDAKGRITHVMEQLEIDWRFSSPIQASDNEIFVKQLIEDQFRYHGLEVLFKAKP
                     IEGVAGNGEHTHLGAAVRLKNNEKVNLFAPNNMQEEYLSEIGYGALMGILKNYEVINP
                     IVSATNDAFNRLKPGFEAPVCTVTSLGGTKDSPSRNRSVLIGVIRSIDNKNTVRFELR
                     SPNPYSNTYLVIAACIQGMLDGIVSVTDAGMDCNLLEKEISKKYGEDAVYLEKFREYR
                     SEKNVFEYYSQDERDKLYGKAPTTVWENINAFASNEIKQLVLKKGDVFTDEIINSFRV
                     STTEKWKNELEGRIIHDNIMLLKTFVKLHSEHDHATDLDVVNWERIVYLKTKLMKDSM
                     TKKCIFTKIKNAISQGDYDIASDLQIQMNEKMSEIRTLYIGYKNNIF"
     gene            1658..3157
                     /gene="nhaC"
                     /locus_tag="PKH42_08720"
     CDS             1658..3157
                     /gene="nhaC"
                     /locus_tag="PKH42_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOA20556.1"
                     /translation="MSKVPREKKEATLGISLIPVLVIVAVLAYSIIVLGADPHIPILV
                     GAAVGSLIAVFGLGYSWEEVEKGIIDSIGSVMQAIIILAIIGMLIGTWIGGGVVPTLI
                     YYGLQILSPTFFLITALLLCSVVSLATGSSWTTAGTVGVALIGIAQGLGVSPALAAGC
                     IVSGAYFGDKMSPLSDTTNLAPAMAGAKLFDHIRHMMYTTGLSYGIAAILYIILGLQY
                     RGKSIDAGEIQSILTAIESIYYINPLFLLVPVITIGAVAMKIPAIPGLLLGVVLGSFC
                     MFFQGSGDIGSIIDAIHYGVAAESGHAMVDELLSGGGMDSMMWTISLIMCALTFGGVL
                     ESTGMMASIANNMLALAKKTGSLILVTILSCLFVNILCADQYLGIALPGKMFKDAYAK
                     RGLAPRNLSRALEDSGTVTSALVPWNTCGATMSSFLGVSTFAYAPFAFFNLLSPIVSV
                     IYGFTGFSIMTIEEDPSSPEYKQKMKVKKSPKELEEYIANYQAKARLAE"
     gene            3271..5358
                     /locus_tag="PKH42_08725"
     CDS             3271..5358
                     /locus_tag="PKH42_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA20557.1"
                     /translation="MGYGSMFEKELNKLIESEDNIECIKDIISNDISNTEQNKEYIER
                     LLEFSTENKLYRSEAWGYYFKGWHYIDNSEYEKAVENFMISYELFDKLNNKYEIAYAC
                     NGLTNVYCLMGQYKLSNEWGLKGISFCEETKNSKAMIILLINTCINYIQMEYYDKALE
                     IIESIEEIDYDLTKSQEISHMLAKAEIEINIGNPDKALKNIEQAMKIESVNNIKSTMG
                     EMLKLKGMAYLKTGKYDLAEDQFKKSYNFCVKYDLIYEKCSTMLQWSKLCILTDNYEK
                     AIDLLNQIIIVGSSNKFNTIMREAFHSLYSIYKKLEDTEKALNYLEKYIKINNEMYDY
                     EQSQLMAEINLNNTKRMAEQYKQLYKRTELLSTIGQKIISNLDISSIIRIINEEIKKL
                     IDLDYFGIAVYDSENNRSEYYFMRDYKIVCSTVNYDEENTLGGYCIKNKEDIIIGNEK
                     LEFKKYLYSEPNYIRGFEEEGVKISSIIYTPMIINDKVVGLMTAQSEKENLYGKDELN
                     ILKILANYTAIAVENAKVYKEIENIATYDSLTNFLTKNEILKIGDSIYEKAKCNNQKF
                     TVIMMDLDDFKIVNDTYGHVYGDKAMSLIAETMSKCIRNTDYIGRFGGDEFLLICTGT
                     GLEEALYVAERIRKTIQSSIFVLSDEIKVRVTTSLGVHEYDENDNSFTDVVKRADQCL
                     YLAKETRNTVMSK"
BASE COUNT         2005 a          791 c         1132 g         1617 t
ORIGIN      
        1 tgcatcccag gtattaaaag agcaatattg gaaaagaact atgggtactg tcagaacagt
       61 cctggaaaaa ataatgcgct tgttggaatg ctatgactta aagcctgaga tggcacataa
      121 ggaagttgga ggcattacca ataaaattga tgcaaaggga agaataaccc atgtaatgga
      181 gcagctggaa attgattgga ggttttcaag tcccatacag gcttcagata atgaaatttt
      241 tgtaaaacaa ttgattgaag accaatttag atatcacgga ttggaagtat tattcaaagc
      301 caaaccaata gaaggcgttg caggtaacgg agagcatacg cacttaggtg cagcagtgag
      361 gcttaagaat aatgaaaaag taaatttatt tgctcctaat aacatgcaag aggaatattt
      421 aagtgaaata ggctacggag ctcttatggg catactgaaa aattacgaag ttataaatcc
      481 gatagtgtct gctacaaatg atgcctttaa caggcttaaa cccggatttg aagcacctgt
      541 gtgcactgta acttccttag gaggtacaaa ggattcacct tcgagaaacc ggtcagttct
      601 tatcggtgta atcagaagta tagacaataa aaataccgta agatttgaat taaggtcacc
      661 aaatccatat tccaacacct accttgttat agctgcatgt attcagggga tgttagacgg
      721 tattgtttca gtaacagatg caggcatgga ctgtaatctc ttagagaaag aaatatcaaa
      781 aaaatacggt gaagatgcag tctaccttga aaagttcaga gaatacagaa gtgaaaaaaa
      841 tgtatttgag tactattcac aggatgaaag agataaactc tacgggaagg ctccaacaac
      901 cgtatgggaa aatattaatg catttgcttc taacgaaata aagcagctgg ttttgaaaaa
      961 aggcgatgtt tttactgatg aaattattaa ttctttcaga gtatccacaa ctgaaaagtg
     1021 gaaaaatgaa cttgaaggaa gaataataca cgataatatc atgttgttaa aaacatttgt
     1081 gaaactccac agtgagcatg accatgctac ggatttagat gtagtaaact gggagagaat
     1141 tgtctattta aaaacaaaac taatgaagga cagtatgact aaaaagtgca tatttacaaa
     1201 gattaaaaat gcaatttctc agggagacta tgatattgct tctgatttac aaatacaaat
     1261 gaatgaaaaa atgtctgaga taaggacact gtatattgga tataaaaata acatatttta
     1321 agtaagaggg gcatacttta aatagggaga ctctttacat taaaactatt taatgtatcc
     1381 ctttcttttt ttgaacatac taatgaaggg ggtttttgtc ttatttttta tacttatata
     1441 taaattttat gcaaaaaatg gtgtttacaa aactataaac atatggtaga attacggcaa
     1501 caaacgtatt cttacttatt ggatagtaag tctacggtta aaaaataatc gcacataagt
     1561 caggataacg tttattattg ggtatgggtt ggactattat atgaattagc attaatttgt
     1621 cctgagatta tgtcgaacaa aataatggag gagaataatg agtaaggttc caagagaaaa
     1681 gaaggaagca acattaggaa tttcgcttat cccggtactt gttatagtag ccgtattggc
     1741 gtattcaata atagtgttgg gagcagaccc gcatattcct attttggtag gagcagcggt
     1801 aggatcactg atagcagttt ttggattagg ctatagctgg gaagaagttg aaaagggtat
     1861 aattgactca ataggaagcg ttatgcaggc aattataata cttgccataa tcggtatgtt
     1921 gattggaaca tggattggcg gcggtgtagt accgacatta atttactacg gcttgcagat
     1981 attatcgcct accttcttct taattactgc cttgttgttg tgcagcgttg tttcattggc
     2041 tacaggaagt tcatggacta ctgcgggaac ggttggagtt gcacttatag gtatagctca
     2101 aggacttgga gtttcaccgg ctttggccgc aggatgcata gtatcgggag catatttcgg
     2161 cgacaagatg tcacccttgt cagatacaac aaacttggct cctgcaatgg ctggcgcaaa
     2221 attgtttgac cacatcagac acatgatgta tacgacaggt ctttcatacg gaattgcagc
     2281 tattctatac attatattag ggctgcaata caggggtaaa tcaattgatg caggagaaat
     2341 tcaaagtatt ttaacagcaa ttgaaagcat ttattatatc aacccattat tcttattggt
     2401 tcctgttatt acaatcggcg ctgttgccat gaaaatacct gcaatcccgg gacttctgtt
     2461 aggtgttgta ttaggatcat tctgtatgtt tttccagggc agcggagata taggaagcat
     2521 aatagatgct atccattacg gagtagctgc agaatcaggt catgctatgg ttgatgagtt
     2581 gttgtcaggc ggaggtatgg atagcatgat gtggaccata tcactgatta tgtgcgcttt
     2641 gaccttcggc ggtgttcttg aatcaacagg catgatggca agcattgcaa acaatatgct
     2701 tgcacttgca aagaagacag gttcattgat tttagtaacg atattatcct gcttgtttgt
     2761 aaacatcctg tgcgccgatc aatacttagg tattgcttta ccgggtaaaa tgtttaagga
     2821 tgcttacgct aaaagaggtt tggcaccgag aaacttgtct cgagctctcg aagattcagg
     2881 aacagttaca tcagcccttg ttccatggaa tacatgcggg gctacaatgt cttcattcct
     2941 gggtgtaagt acatttgcat acgcaccatt tgcattcttt aaccttttaa gcccgattgt
     3001 atcagttatt tacggtttca ccggattctc aattatgaca attgaggaag acccgtcatc
     3061 acctgaatat aagcaaaaaa tgaaggttaa gaaatcaccg aaagaattgg aagagtatat
     3121 agcaaattat caagctaaag caagattagc tgaataataa agaaaatcaa aaaccttgcg
     3181 ggagtccgca aggttttttt gtaataagta tttgacaatt tttagcgttc aatatattat
     3241 atttctaatc gattctcaag gaggataaaa ttgggttacg ggagtatgtt tgaaaaagaa
     3301 ttaaataaac tgattgaaag cgaagataat attgaatgca taaaagatat aatttcgaat
     3361 gacataagca atacagagca gaataaagaa tatattgaaa gattgttgga attttccaca
     3421 gagaataagc tatacagatc cgaagcgtgg ggttattatt ttaaaggctg gcattatatc
     3481 gataattctg aatatgaaaa agcagtagaa aattttatga tttcttatga gctgttcgat
     3541 aagctaaaca ataaatatga aatagcgtat gcctgcaatg gattgacaaa tgtttattgt
     3601 ttgatgggac agtataaatt atcaaatgaa tggggtctta aaggcatttc gttttgtgaa
     3661 gaaacgaaaa actctaaggc aatgattatc cttcttatca atacatgtat taactatatt
     3721 caaatggagt attatgacaa agcgttagaa attatagaaa gtattgaaga aatagattat
     3781 gatttaacaa aaagtcaaga aatttcgcac atgctggcaa aagccgaaat tgaaataaat
     3841 atcggcaacc ccgacaaagc ccttaaaaat attgagcaag cgatgaaaat agaatcagta
     3901 aataatatta aatctaccat gggtgaaatg ttaaaactta agggtatggc ttatttaaaa
     3961 acaggtaaat atgatttagc tgaagaccag tttaaaaagt cttataattt ttgtgttaaa
     4021 tatgatttga tatatgaaaa gtgctccacc atgctgcagt ggtctaaatt atgtattttg
     4081 actgataatt atgaaaaggc aattgactta ttaaatcaaa ttattattgt cggttcatca
     4141 aataagttta acacaataat gagagaagca tttcattctt tatattctat ttataaaaaa
     4201 ctggaagata ctgaaaaggc attgaattac cttgaaaagt atataaagat aaataatgaa
     4261 atgtatgatt atgaacaaag ccagcttatg gctgaaataa atttgaacaa tacaaaacgt
     4321 atggcagaac aatataaaca gctttataaa aggactgaac ttctttcaac catagggcag
     4381 aaaataatat caaatttaga tattagttca attatccgca tcataaacga agaaataaaa
     4441 aagctgattg atctcgatta ttttggaatt gctgtgtatg acagtgaaaa taaccggtcg
     4501 gaatattatt ttatgagaga ctataaaata gtttgcagca ctgtaaatta tgatgaggaa
     4561 aatactctgg gaggatattg tattaaaaat aaggaagata ttattatcgg aaatgaaaag
     4621 ctggaattta agaaatactt atattctgaa cccaactata tcagaggatt tgaagaggaa
     4681 ggcgtaaaaa tttcttccat tatttatact cccatgatta taaatgacaa agttgtcgga
     4741 ttgatgactg ctcaaagtga aaaagaaaac ctttacggca aggatgaatt gaatatcttg
     4801 aaaattctag caaattatac tgcaattgct gttgaaaatg ccaaggtata caaagaaatc
     4861 gagaatattg caacttatga cagtctgaca aattttctga ctaaaaatga aatactgaaa
     4921 attggagata gtatttacga aaaggccaag tgcaacaatc aaaaatttac cgtaattatg
     4981 atggatttgg atgactttaa aatagtaaat gatacatacg gtcatgttta cggagataaa
     5041 gcaatgagtc ttattgccga aaccatgtca aaatgcatta ggaatactga ctatatagga
     5101 agattcggag gagatgagtt tttgctcata tgtaccggaa caggattaga agaagcctta
     5161 tacgttgccg aaagaataag aaaaacaata caaagcagta tatttgtatt aagtgacgaa
     5221 ataaaagtaa gggtaacaac gagtttaggg gtacatgaat atgacgagaa tgacaactca
     5281 tttaccgatg tggtaaaaag agcagaccaa tgcctctatc ttgcaaaaga aacaagaaat
     5341 accgttatga gtaaataaat aacaaacccg gaggggaaaa cccggagggg acggttctca
     5401 gtggacaacc gtcccctttg ggtccaaatt agacataaca gtgtcaaaat tattatgatt
     5461 aaatttattg ctgaggggta cactaatttt gacattttaa ataggagaac aatatgaaaa
     5521 agattctgag aataaattta agaac
//
LOCUS       DAOPUE010000122         5414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_15104_length_5414_cov_12.095727, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000122 DAOPUE010000000
VERSION     DAOPUE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15104_length_5414_cov_12.095727"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1180)
                     /locus_tag="PKH42_08730"
     CDS             complement(95..1180)
                     /locus_tag="PKH42_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOA20558.1"
                     /translation="MDTIIMTIVIFSGISGVLAAILTISDRTIGNYGEVTLTINEDKI
                     YTVKGGSSLLDTLRNEQIFIPSACGGKGTCGYCKVVVLEGGGPVLATEKPLLTKEELE
                     NNTRLSCQCKVKQNIKAKIPEELFNVKEYAVIVEKMEQLTPTIKKLRFNLGEEEISFK
                     PGQYVQLKAPAYEGNDEEVYRAYSLASSVNDKHAIELLIGFTGGIATTYVHQHLKEGD
                     MAYINGPYGEFYYHEDDGPIIFAGAGTGMAPIISILQYMADNNIMREALFFFGARTMN
                     DLFLVDKIKGFEEKLNLRFIPTLSREESPEWKGEKGRVPNAIDKYIENGENYSAYLCG
                     SPAMIDSIVEALVKKNVPADKIYFDKF"
     gene            complement(1198..1788)
                     /locus_tag="PKH42_08735"
     CDS             complement(1198..1788)
                     /locus_tag="PKH42_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOA20559.1"
                     /translation="MIQVHPFIIFIASIFTSNMIFSNFLGMCSFIAVSKEIPTALGLG
                     IAVTMVLSATTVLNYLVYWYIIMPLGLEYLMYILFILVIAAFVQLLEMTMDRYLPTLY
                     YALGIFLPLITVNCAILGVSLFMVTRQYNFFQTFGFAVGSGIGWALAITAMAGIRSKL
                     NENSIPEGLRGVPITLIITGIMALAFIGFSGMVQIQ"
     gene            complement(1785..2408)
                     /locus_tag="PKH42_08740"
     CDS             complement(1785..2408)
                     /locus_tag="PKH42_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOA20560.1"
                     /translation="MTKTEVKKIFKKGIYTDNPIFVQVIGICSALAVTNLMINSLIMG
                     IALALVTAFSSLTVSLLKDHTPKHIRMMVQVLIISAYVIMVDIYLKAYMPEISKALGP
                     YVGLIITNCIIMGRAEGFARSNPPIPSFFDGLASGIGYTFVLMSISFIREILGFGSIF
                     GISIIPETFTRWTLMVMPPSAFFILALIMWVFNNNRLKKQREGVERK"
     gene            complement(2410..3003)
                     /locus_tag="PKH42_08745"
     CDS             complement(2410..3003)
                     /locus_tag="PKH42_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOA20561.1"
                     /translation="MKNSFIYPIIFMMAVTAVFISVLAGLNFVTADTISFNQESQLQQ
                     KILYIFDILPEGGTEKDIERVFNDSVIEKEWGELRGYALIQGGKETAYAIPVNGAGLW
                     GSIIGYIGLNADYTEIIGIEFVTQNETPGLGGRISEDSYKEQFRGIDISGKSENFIIS
                     SPEPGSNVDAIAGATQTSNAVVKLINDDIKIFLREVE"
     gene            complement(3000..3956)
                     /locus_tag="PKH42_08750"
     CDS             complement(3000..3956)
                     /locus_tag="PKH42_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOA20562.1"
                     /translation="MNVLNLDFRKQKMMRTVMLSLIPIIIFSVYNYGLRVLALLTVIT
                     ITGTAVEYLWEKHYNNKPSEAIFVTCILYTMTLPSSIPYWIAVIGIIFGVIFGKMSFG
                     GFGRNVFNPALVGRAFIYINFPNPMTIVWNEASSGFPGGFSTYLTAGIDAVSEATPMI
                     TYNYTGEIMDLNSLILGNIPGAIGESYKILIILAAIYLIYTKTASLEIMLGCLIGFFT
                     VGTFLYYIYDGINPIYGMMMGGFLFGTVFMATDPISAAKTKKGKWIFGIIIGVVTVLI
                     RALSLFNGGMMFAILMGNTFAPIIDHFIKEAENRKKSKAVAA"
     gene            4285..4653
                     /locus_tag="PKH42_08755"
     CDS             4285..4653
                     /locus_tag="PKH42_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein COQ7"
                     /protein_id="HOA20563.1"
                     /translation="MAGFGNPFGANVPRKLTKDELIQAVRLNIAGELEAIYVYDAHIQ
                     ATDDIVAKKIIGDIRDEEKAHVGELLTLLKYLDPKEAEFLNEGEEEVREMLEELNINM
                     ETAPSTDTNSSSTVGSLLKE"
     gene            4663..>5414
                     /locus_tag="PKH42_08760"
     CDS             4663..>5414
                     /locus_tag="PKH42_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOA20564.1"
                     /translation="MDFLSREGSPISAELWSKIDEQVISMAKKVLVGRRFLHIYGPLG
                     AGVQSINIDDSTQLSETEGDIVQIKGRKFEQIPLLNQDFSLLWRDLELSEKTGMPIDL
                     SSALQASAEISKKEDELIFNGNKELGYEGLLTAKGITKLELSNWEEGENPVKDITAGM
                     TNLFDKGLVGRKALIISSDLLIKLQRIQPGTGITEYERLSKLVDGNIYYTKVIGSKKA
                     VLVCAEPQYMDLVVGQDMATSYLETKDLNHYF"
BASE COUNT         1834 a         1080 c          894 g         1606 t
ORIGIN      
        1 acttagtaaa gaagctggca aaagctatac aatagcaaaa aaaggcggtg ccaggcacct
       61 ttattggtgc ctggcaccac gtatgtggca ccgcctaaaa cttatcgaag tatattttat
      121 ccgcaggcac attctttttc accaatgctt caactatgga atcaatcatt gcagggctgc
      181 cgcaaaggta tgcagagtaa ttttcaccgt tttcaatata tttatcaatt gcattaggta
      241 cacgcccctt ttctcccttc cactcagggg attcctcccg agataatgta ggtataaacc
      301 ttaggttaag tttttcttca aagcccttta ttttatcaac taagaacaaa tcattcattg
      361 ttctggctcc aaagaaaaat agagcctccc tcattatatt attatcagcc atatactgca
      421 gaattgatat tataggcgcc attccggttc cggcacctgc aaatataatc ggaccatcat
      481 cctcatgata ataaaactcg ccataaggac cattaatata agccatatcc ccttccttta
      541 aatgctgatg cacataggtg gtggcaattc ctcccgtgaa acctatcaat aattcaatag
      601 catgtttatc atttacagaa gatgcaagag aatatgccct gtacacttct tcatcatttc
      661 cttcataggc gggagcttta agctggacat actgtcccgg tttaaaactt atttcttcct
      721 ctcccaaatt aaaacgaagt ttttttatgg tcggtgttaa ttgctccatt ttttctacaa
      781 taacggcata ttctttaaca ttgaacaatt cttcgggaat ttttgctttt atattttgct
      841 taaccttgca ttggcaggac agccttgtat tattttctaa ttcctctttt gtaagaagag
      901 gtttttcggt tgcaagaaca ggtcctcctc cttcaaggac aacaaccttg caatacccgc
      961 aggttccctt tccaccgcat gcagaaggta taaaaatttg ttcatttctt aatgtatcta
     1021 acaaactgct gccgccctta acggtataaa ttttatcttc atttatcgta agagtaacct
     1081 ctccgtaatt gcctatggtt ctgtcggaaa tagtcagtat tgctgctaga actcctgaaa
     1141 ttccggagaa tattacaatt gtcataatta ttgtatccat aatgcctcct acgcctccta
     1201 ctgtatttga accataccgc tgaaaccgat gaatgctaag gccataattc cggtaattat
     1261 taaagtaatc ggaactcccc ttaacccctc cggtatggag ttttcattaa gctttgacct
     1321 tattcccgcc atcgcagtta tggcaagagc ccaaccgata cctgagccta ctgcaaaacc
     1381 aaaagtctgg aaaaaattat actgcctggt aaccatgaac aatgaaactc caagtatggc
     1441 acaattaacg gttataagag gcagaaaaat tcccaaggca tagtatagag tcggtaaata
     1501 tctgtccatt gtcatttcca aaagctgtac aaatgctgca ataaccaaaa tgaacaatat
     1561 atacattaag tattccaatc ctaagggcat tataatgtac caatatacaa gataatttaa
     1621 cacggttgta gccgaaagca ccattgtaac agcaattccc aagcccaagg cagttggtat
     1681 ttcctttgaa acggcaatga atgagcacat ccccaaaaaa tttgaaaaaa tcatgttgct
     1741 tgtaaaaata gaagcaataa atattataaa aggatggacc tgaatcattt tctctcaact
     1801 ccttctctct gtttttttag acgattgttg ttaaaaaccc acattattaa tgcaagtatg
     1861 aaaaatgccg aaggaggcat aaccattaat gtccaccttg taaaagtttc aggaataata
     1921 ctgattccaa aaatacttcc aaaacctaaa atttctctta tgaaggaaat agacattaaa
     1981 acaaaggtat aacctatacc ggatgcaaga ccgtcaaaaa atgacggtat tggcggattg
     2041 gaccgggcaa atccttcagc ccttcccatt atgatacaat ttgtaattat aagtcctaca
     2101 taaggtccta atgctttact tatttcaggc atgtaagctt tcaaataaat atcaaccata
     2161 attacatatg ctgaaataat taatacctgt accatcatac gaatatgttt gggagtgtgg
     2221 tctttaagca gagataccgt caatgaagaa aatgcagtta ccagagcaag agcaattccc
     2281 ataatcagcg aatttatcat taaattagtt acagctaatg cagagcaaat tcctattacc
     2341 tgcacaaata tcggattgtc ggtgtatatt ccctttttaa atatcttttt tacttctgtc
     2401 ttggtcaatt tattccacct cccgtaaaaa tatctttata tcatcattta tgagctttac
     2461 aacggcattg gacgtttggg ttgcaccggc aatagcatca acattgctgc ccggctcagg
     2521 agatgaaata ataaaatttt ctgacttacc ggatatatct atacccctaa actgctcctt
     2581 gtatgaatcc tctgatattc tgccgccaag tcccggggtt tcattttgtg ttacaaactc
     2641 aatacctata atttctgtgt aatctgcatt taatccaata tatcctatta tggagcccca
     2701 aagtcctgca ccgttaacag gaattgcata tgctgtttct ttaccgcctt gaatcaatgc
     2761 ataacctctt agctctcccc actctttttc aattacacta tcattaaata ctctttctat
     2821 atccttctcc gtcccgcctt ccggcaatat atcaaaaata taaagaattt tttgctgcag
     2881 ctggctttct tgattaaagg aaattgtatc tgcggtaacg aaattcaaac ctgccaagac
     2941 tgaaataaat actgcggtaa ccgccatcat aaatattatt ggataaataa aagaattttt
     3001 catgctgcca ccgccttact tttctttcta ttttcggctt ccttaataaa atgatcaatt
     3061 atgggtgcaa atgtattacc catcaatatt gcaaacatca taccgccgtt aaaaagcgac
     3121 agagcccgaa taagaacagt tacaacacct attattattc caaaaatcca cttgcccttt
     3181 tttgttttgg cagcactgat ggggtcggtt gccataaaaa cagtaccgaa caaaaatcca
     3241 cccatcatca ttccatatat gggatttata ccatcatata tataatacaa aaaagttccg
     3301 actgtaaaaa acccgattaa gcagcccagc ataatctcaa gggatgcagt ctttgtatat
     3361 attaagtaga tagcagccaa tataatcaat attttatagc tttctccaat ggcacccgga
     3421 atgtttccta atatcaaaga atttaaatcc ataatttcac cggtataatt atatgtaatc
     3481 attggagttg cttctgatac agcatcaata cctgctgtta aatatgtaga aaaaccgccc
     3541 ggaaaaccgc ttgatgcttc attccatact attgtcatgg gatttggaaa gttaatataa
     3601 ataaaagccc gcccaaccaa agcaggatta aaaacatttc tgccaaaccc tccaaaagac
     3661 atcttaccaa aaattacacc aaaaataatc cctataacag caatccaata aggtatggac
     3721 gaaggaagcg tcatagtata caatatgcag gttacaaata tggcttcaga cggcttattg
     3781 ttataatgct tttcccacaa atactcgaca gccgtacctg taatcgttat cacagtaagc
     3841 aaggctaaga ctctcaagcc ataattataa acggaaaata taattatcgg gataagagac
     3901 aacataaccg tcctcatcat cttttgtttt ctgaaatcta aatttaaaac attcaatcta
     3961 tccctccatt cagcatatat aaaatatact taagctacat aatagtctta tacccgaata
     4021 atcttggtta taacagtcat tatataaaaa tttgtaaaaa gctgggttat ccattctgaa
     4081 cttatttgtc atcctgagct tagttgtcat cctgagttaa ttgtcatcct gagcttagtt
     4141 gtcatcctga gcgttagcga aggatctcat cttttaatac ttttcattac tcccatttca
     4201 acaaataaaa atttatttaa tgtttataaa tgcttttttg tggtatataa ttaatgttaa
     4261 acatttattt tagaggagga aactatggca ggttttggaa acccatttgg tgctaacgta
     4321 ccacgaaaat taactaagga tgaacttatt caagcagtaa gactaaacat tgcaggtgag
     4381 ctggaggcaa tctacgtata tgatgcacat attcaagcca ccgatgacat tgttgcaaaa
     4441 aaaatcattg gagacataag agatgaagaa aaagctcatg taggtgaact attaacacta
     4501 cttaagtatt tagaccctaa agaagcagaa ttccttaatg aaggagaaga agaagtaaga
     4561 gaaatgcttg aagagcttaa tattaacatg gagacagcac catcaacgga tacaaatagc
     4621 agtagtaccg taggaagctt attaaaagaa taggaggata gtatggattt tttatcaaga
     4681 gaaggctctc cgatttccgc agagctttgg tcaaaaatag atgaacaagt aatatcaatg
     4741 gctaaaaaag tacttgtggg aagaagattc ctgcatattt acggtccctt aggggcagga
     4801 gtacagagca taaatataga tgacagtaca caattatcag aaactgaagg cgatatagtt
     4861 caaataaaag gaagaaaatt cgagcaaata cctttattaa atcaagattt cagcctttta
     4921 tggcgagact tggaacttag cgaaaaaaca ggaatgccta ttgatttgtc ttcagcatta
     4981 caagcaagcg cagaaatatc aaaaaaagaa gatgaattaa tttttaacgg caataaagaa
     5041 ttaggttatg aaggacttct tacggctaaa ggaattacaa aattagagct tagcaactgg
     5101 gaagaaggag aaaaccccgt aaaagacata acagcaggta tgacaaattt atttgataag
     5161 ggattagtgg gaagaaaagc attaattata agctcggacc tattaatcaa acttcaaaga
     5221 attcaaccgg gaaccggaat tacggaatac gaaagactaa gcaaattagt agacggcaat
     5281 atttattaca caaaagtaat aggaagcaaa aaggcagtcc tagtttgtgc cgagccgcaa
     5341 tatatggatt tggttgttgg tcaggacatg gcaacatctt atcttgaaac aaaagattta
     5401 aaccactatt tcag
//
LOCUS       DAOPUE010000123         5393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_15206_length_5393_cov_8.848070, whole genome shotgun sequence.
ACCESSION   DAOPUE010000123 DAOPUE010000000
VERSION     DAOPUE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15206_length_5393_cov_8.848070"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1248
                     /locus_tag="PKH42_08765"
     CDS             196..1248
                     /locus_tag="PKH42_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOA20565.1"
                     /translation="MEIPANKILSPSELSFFCMQIAMILKSGMLISDGIDWMYEDIKE
                     GNVKDALHYLKNKLSEKVPLYVAMKNSGYFPSYIVNMSQIGSITGKLEDVMLSLSEYY
                     ERERLLKEKIRNSIFYPAMLFIMMSFIIILLVTKIFPIFEGMLEKLGASSNDSSSLSF
                     NKGIMAGRISMILVIIIIAVIAFIYILYRSEKGKKNLNMFLSNFALTKSIMKKITAYR
                     FSSAMSLLLNSGMNVDNSIDILLDIAEEPVFKENIKLCKDSMKSGDSFLDSINKLSLF
                     SSMHMQMLHMGEKIGEMDVVMNKLTDIYENEAEQAISNTVSLIEPVLVGILSVLTGVI
                     LISVMLPLMNIMSSIG"
     gene            1266..1571
                     /locus_tag="PKH42_08770"
     CDS             1266..1571
                     /locus_tag="PKH42_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20566.1"
                     /translation="MRKNFKFIFVLSIIILIAYMYIAVDSAQRKSDEESYKILSDAII
                     RSAVQCYAIEGFYPPDIEYLENNYGLLVDHNKYFVSYSVFASNIIPEVQVFLKNPNQ"
     gene            1587..2069
                     /locus_tag="PKH42_08775"
     CDS             1587..2069
                     /locus_tag="PKH42_08775"
                     /inference="COORDINATES: protein motif:HMM:NF027478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HOA20567.1"
                     /translation="MDRQNQFSFQFIFIMLLYLVIVILSVIVISLGKNIYDKINEDRN
                     KNYELQVSLSYIANKIRQSDINDAVEIKDLNGIDAVVINEVYDEEKYQTWIYFYDGAI
                     YEMFTDAGSTFELSDGSKVVEADLFKIEKVKDNLYKFTAVSKEENSELYLSLYSKAVT
                     "
     gene            2069..2554
                     /locus_tag="PKH42_08780"
     CDS             2069..2554
                     /locus_tag="PKH42_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20568.1"
                     /translation="MNKVSLNSKHSLLEIILSVLIFLAAGIIMLNCFGIARFTQIRAN
                     DKVTAGAIIQSDFEIIKSLNSTDELYEFLNTYGTKESGSINTYTKYYDENWIQGSGKE
                     YAVTIVIDDENTSSGTLININISAQKNNPYPFIKAGGQQIYSIESKKFFPSFGGAYED
                     K"
     gene            2541..3011
                     /locus_tag="PKH42_08785"
     CDS             2541..3011
                     /locus_tag="PKH42_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20569.1"
                     /translation="MRISKINVSVGIGGTLIITIFVVLCLMIFAVLSFVTAYSDLKLS
                     NKAQEITADYYYIHGKAEEKLAEISDVLYGIDINEEMDNILKKLSDIDGVYVDEENFK
                     LQYEVFGEENQKISVTLNLLHKDRFFYEIEAWNLSKIELPVYEDEIIDIWEGIE"
     gene            3013..4074
                     /locus_tag="PKH42_08790"
     CDS             3013..4074
                     /locus_tag="PKH42_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOA20570.1"
                     /translation="MDIINIFKTAIEKKASDIFIIAGRPLCYKILGDIVTFDEGILTS
                     DDTFKIISDIFNIANEKDLSVLEDKGDADFSFSLQKLGRFRVSAYKQRNSYAAVIRVV
                     FFELPDPKKLGIPDVVMDLYKKTKGLVLITGPAGSGKSTTLACIIDKINQERNCHIMT
                     FEDPIEFIHKHKKSIVSQREIYTDTNSYISSLRSALRQAPDVMLIGEMRDLETIEIAL
                     TAAETGHLVLSTLHTTGAANTIDRIIDVFPSNQQQQVRVQLSMQLQAVISQHLIPSVP
                     SGRVAAFEVMLTNNAVSNLIRESKVYQLNSIIHSSEYMGMKTMDASILELYKKGLITK
                     ENALIYATEPDSLLKQLNR"
     gene            complement(4190..5068)
                     /locus_tag="PKH42_08795"
     CDS             complement(4190..5068)
                     /locus_tag="PKH42_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOA20571.1"
                     /translation="MRQMRKITIILMIVLFIASFTYCYGADNIYKGTKSEIDASNVED
                     GYVKIKYLKETTKKLKVIIEKDKGKYTYDLNNKGEYDVYPLQMGDGKYKIRVFENISG
                     NKYATKQTVTINVKLKEDKVPFLVPNQLVNFTNTSETVKKAAELTEGITDEFGKIDAI
                     YDYIITNIKYDTEKASTVKSGYLPTVDEILKTSKGICFDYASLMAAMLRSQGIPAKLV
                     TGYSSNLNVFHAWNEVYTEETGWIKLNEMYFDGFQWKLMDSTIASSGKQSNSPKVMEY
                     TNKLIDSKFYTKQFEY"
     regulatory      complement(5180..5266)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1798 a          849 c          927 g         1819 t
ORIGIN      
        1 ttgttagttt atcataactg caatttaaag tcaacaggtt ttcaacttga tttttatctt
       61 ttttgtagac tttttttgag atgattcttt ttcgttgtac ccccgtcccc ttgtcatatt
      121 cttatgtaga attttgttga attttaaata tatctataat ataattatta aaaagtaaaa
      181 agttgtgagg ttattatgga aataccggca aataaaatct tatctccttc tgaattatca
      241 tttttctgca tgcaaattgc aatgatatta aaatcaggaa tgcttatatc cgacggcatt
      301 gattggatgt atgaagatat aaaagaagga aatgtcaaag atgctcttca ttacttaaaa
      361 aacaagttgt ccgaaaaggt tcctctttat gttgctatga aaaactccgg atatttccct
      421 tcatatatag taaatatgag tcaaatagga agtataaccg gtaagcttga agatgtaatg
      481 ctttctttat ccgaatatta tgagagagaa agactcttga aagaaaaaat caggaattct
      541 attttttatc cggctatgct ttttattatg atgtctttta taattatatt attggtaaca
      601 aaaatttttc ctatatttga agggatgctt gaaaagcttg gtgcttcttc aaatgattca
      661 tcttctcttt ccttcaacaa gggtattatg gccggaagaa tttccatgat actagtaatc
      721 ataataattg cagttattgc ttttatctat attttatata gatccgaaaa gggcaaaaaa
      781 aatcttaaca tgtttttaag caactttgca ttgactaaat caataatgaa aaaaattacc
      841 gcatacaggt tttcatctgc tatgtctctt ttgttaaaca gcggtatgaa tgttgataat
      901 tcaattgata ttcttctcga tattgcagaa gagccggttt ttaaagaaaa tattaaattg
      961 tgcaaggact caatgaagtc gggagatagt tttcttgatt ccatcaataa attatctctg
     1021 ttttcaagca tgcatatgca aatgcttcac atgggtgaga aaatcggtga gatggatgtt
     1081 gtaatgaata aacttaccga tatatatgaa aatgaagccg aacaagcaat cagcaacact
     1141 gtttcattga tagagcccgt cctggtagga attttgtcgg ttttgacagg tgttatactg
     1201 atttctgtta tgcttccttt aatgaacata atgtcatcta taggataact gaaaggaatg
     1261 atattatgag aaaaaacttt aaatttattt ttgttctgtc aataatcatt ttgatagcct
     1321 atatgtacat agctgttgat agtgcccaaa gaaaaagtga cgaggaaagt tacaaaattt
     1381 tatctgatgc aataataagg agtgccgttc aatgctatgc tattgaagga ttttatcccc
     1441 ccgatataga atatcttgaa aataactacg gtttgttagt agaccataat aaatattttg
     1501 taagctacag tgtttttgct tccaacataa ttcccgaagt acaggtattt ctaaaaaacc
     1561 cgaatcaata ataggaagga aaaaatatgg acagacaaaa tcaattttca tttcaattta
     1621 tattcattat gcttctttac cttgttattg taatcctgtc tgtaatagtt atttctttgg
     1681 ggaaaaatat atatgataag ataaatgaag acagaaacaa aaattatgag ctgcaggttt
     1741 ccctttcata catagcaaat aaaatcaggc agtctgatat aaatgatgct gttgaaatta
     1801 aggatttgaa cggtatcgat gccgttgtga taaatgaagt atacgatgaa gaaaaatatc
     1861 aaacttggat atatttttat gacggtgcga tttatgaaat gtttaccgat gcaggcagta
     1921 cctttgaatt gtctgacggc tctaaggtag tggaagcgga cctctttaaa attgaaaaag
     1981 tcaaggataa tttatataaa ttcactgctg taagcaagga agaaaactca gagctgtatt
     2041 tatccctgta ctcaaaggcg gtgacttgat gaataaagta tccctgaatt caaagcattc
     2101 acttttggaa ataattttat ccgttttaat atttttagcc gccggaataa taatgttaaa
     2161 ttgcttcggc atagcaaggt ttacacaaat aagagcaaac gataaggtaa cagccggtgc
     2221 aataatacaa tcagattttg aaattataaa atcattaaat agcaccgatg aattatatga
     2281 atttctgaac acttacggca caaaagaatc aggaagcatc aatacataca ccaaatatta
     2341 cgatgaaaac tggattcaag gcagcggtaa ggaatatgcc gtaaccatag taatagatga
     2401 tgaaaatacg agctcaggca cattgataaa tataaatatt tctgcccaaa aaaataatcc
     2461 ctatcctttt attaaagcag ggggacaaca aatttactct attgaaagca aaaagttttt
     2521 cccttccttt ggaggtgcct atgaggataa gtaagattaa tgtttctgta ggaattggag
     2581 gaactctaat aataactata tttgtagtgc tgtgcttaat gatttttgct gtattgtcat
     2641 ttgtaactgc ttactcggat ttgaaactgt ccaataaagc tcaggaaatt acggctgact
     2701 attactatat tcacggcaag gctgaagaaa agctggctga aataagcgat gtattgtatg
     2761 gcattgacat aaatgaagaa atggataata ttttaaaaaa attatccgat attgacgggg
     2821 tttatgttga tgaagaaaat tttaaacttc aatatgaagt atttggagag gagaatcaaa
     2881 aaataagcgt aactttaaat ttacttcata aagacagatt tttttatgaa atagaagcat
     2941 ggaatttatc aaaaatcgag ctgcctgttt atgaagacga aattatagat atatgggaag
     3001 gaattgaata gtatggatat tattaatatt ttcaaaactg ctatagagaa aaaagcttcg
     3061 gatattttta taatcgcagg cagacctcta tgctataaaa ttttaggtga cattgtgacc
     3121 tttgatgaag gaatactaac ttcggatgat acgtttaaga taataagtga cattttcaac
     3181 attgccaatg aaaaagattt gtctgtttta gaggataaag gggatgcgga tttttccttt
     3241 tcactgcaaa agctggggag attcagggta agtgcatata agcagcgcaa ttcttatgct
     3301 gccgtaatca gagtagtgtt ttttgaactt cccgacccta agaaattagg cataccggat
     3361 gttgtgatgg atttatacaa aaaaactaag gggctggttc ttattaccgg tcctgccggc
     3421 agcggaaaat ccacaaccct ggcatgtata atcgacaaaa ttaaccagga gagaaactgt
     3481 catataatga cctttgaaga ccccatagaa tttattcaca agcataaaaa atcaattgtt
     3541 agccaaagag aaatttacac tgatacaaac agttatattt cttctcttcg gtctgcctta
     3601 agacaagctc ctgatgtaat gctgatagga gaaatgaggg atttggaaac tatcgaaata
     3661 gctttaacgg ctgcggaaac cggacatttg gttttatcaa cactgcatac cacgggtgct
     3721 gcaaatacca tcgacagaat aattgatgtt ttcccctcta atcaacagca gcaggttaga
     3781 gtccagcttt caatgcagct gcaggcggtt atttcacagc atctcattcc ttcagtgccg
     3841 tcaggaaggg ttgcggcttt tgaggttatg cttactaaca atgccgtttc caatttaata
     3901 agggaatcca aagtctatca gcttaacagc ataattcatt cttctgaata tatgggtatg
     3961 aaaacaatgg atgcaagtat tttggaatta tataagaaag gtttgattac aaaagaaaat
     4021 gcattgatat atgcaacaga gccggattca ttacttaaac aacttaatcg gtagatagac
     4081 acgcctctaa ctaaaaaatg accctgtttg gtcatttttt agcgtgatga ggagattaat
     4141 tcgaaattag gaaggaaaaa cccgcccccc ttgtaatgcg ggataatcat taatattcaa
     4201 attgtttcgt ataaaacttg ctgtcaatta acttatttgt atattccata actttcggac
     4261 tgtttgactg cttgcccgat gatgcaatgg tactgtccat aagcttccat tgaaagccgt
     4321 caaaatacat ttcgttcaat tttatccaac ctgtttcttc cgtatacacc tcattccatg
     4381 catgaaatac atttaagttt gaagaataac cggttaccag tttagccggt ataccttgtg
     4441 agcgaagcat tgcggccatt aacgaagcat aatcaaagca tattcctttg ctggttttaa
     4501 gtatttcatc tacggtaggc agatatccgc tttttaccgt actggccttt tcagtgtcat
     4561 attttatatt ggttattata taatcataaa ttgcatctat ttttccaaat tcatctgtta
     4621 ttccttcggt taattcggct gctttcttta cagtttcaga tgtattcgta aaattaacca
     4681 attgatttgg aactaaaaac ggcactttgt cttcttttaa tttaacgttt atggttaccg
     4741 tttgtttggt tgcatactta ttaccgctta tattttcaaa tactcttatc ttgtatttcc
     4801 cgtctcccat ctggagagga tatacgtcat attcaccctt attgtttaaa tcgtatgtat
     4861 attttccttt atctttttcg ataataactt ttaatttttt tgttgtttct tttaaatatt
     4921 ttattttaac ataaccatct tcaacattag aagcatctat ttcggatttg gttcccttat
     4981 atatgttatc tgctccataa caatatgtaa atgaagcaat aaataacact atcatcagta
     5041 taattgtaat ttttctcatt tgtctcattg cttgtcttcc tttccttcaa tcttaactat
     5101 agattgtcca cataatttat aataaaaaag ccttgctgaa aacagcaaag ccaaccgtca
     5161 tattaatgta ctcaataagt acagcaactg gctgtcatac cattttcatg ggttagaccc
     5221 tatagctttg cgtcattttc tttagaaaat tttgccacat atttaattta aataataata
     5281 acatgaatcc ttatacatgt caacatattt tgacaatttt tacaaaaaaa tttgaaaccg
     5341 tgaattgaca aagtaaatgc aaccctatga ttcaaatata gttgcattta atc
//
LOCUS       DAOPUE010000124         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_15572_length_5294_cov_7.666539, whole genome shotgun sequence.
ACCESSION   DAOPUE010000124 DAOPUE010000000
VERSION     DAOPUE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15572_length_5294_cov_7.666539"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PKH42_08800"
     CDS             complement(<1..569)
                     /locus_tag="PKH42_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOA20572.1"
                     /translation="MKAIDTLQKLSIPKSLINYKKAYNFFVISFLLILLIVLIFTSIS
                     VGSSSIEISDIFNAISGNGQLTSEYKIINYVRIPRTLSAIIAGSGLAISGLILQSVLN
                     NALASPTIIGVNSGAGLFTALATAFFPANLSIMPLAAFLGAFLATIAVYYVARKTGAS
                     RMTIILAGVAVSSLVGAMTDTVLILFPDTV"
     gene            complement(517..1497)
                     /locus_tag="PKH42_08805"
     CDS             complement(517..1497)
                     /locus_tag="PKH42_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOA20573.1"
                     /translation="MISKKKNLFLILTLILMLITAACSKETNNVEAINIDNQDAEENY
                     YIFSDALGTEVVLERKPERVVSLLGSYSETWALAGGSLAGVTDDVQKEGRMEITNDIQ
                     IVGTVKEPNLEGILSLNPDFVILTPDVDNQVKIAETLKQLNINHAFFKVEEFEDYLNM
                     LNICTDITGRKDLYEENGLKIKKDIDDILYKVEGAKKEELKVLFVRAFSSGAKAKKDD
                     NMTCTMLDNLGTVNIAAQHPSLLEDISIEEIIEEDPDFIFVTTMGDEQKAIDALKTGI
                     GTNPAWNSLSAVENDRYIILPKELFHYKPNAKWGESYRYLAEIIYPEIFN"
     gene            complement(1637..2557)
                     /locus_tag="PKH42_08810"
     CDS             complement(1637..2557)
                     /locus_tag="PKH42_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOA20574.1"
                     /translation="MTIRHLKIFIEVAQCGNMTAAASKLFISQPTASQAIRELEEHYG
                     VLLFERLSKRLYITEAGKKLLFYAKEVVKEFDSLESKMNNLNKKDKIRVGATISVGDC
                     ILSDLIHRCIEENPNVEIYSFVGNTETIEEKLLSNELDIAIVEGKVKSQDLIVKPELE
                     DYLVLICSTKHPFAQKKIIKTDELRDENFAMREKGSGTRELFEEYMLNNGMPIKVVFE
                     GNTPQSIKQEVIHNNCLSVISICLVENEIKDSLIHVIENEENTWNRHFSLVYHKDKLL
                     TDAIKSLINIVKDYRYIPSKAIVNPGILRK"
     gene            2691..4313
                     /locus_tag="PKH42_08815"
     CDS             2691..4313
                     /locus_tag="PKH42_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005978247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOA20575.1"
                     /translation="MNIIIIGGVAAGTKVAAKLKRDNYNHNVKILTKGKDISYAGCGL
                     PYYVGNVIEKQEDLIQNTPKQFSELTGVEVLTEVEVTKVNPEEKTVEALNLITNETAT
                     YSYDILVIATGAEPIKPPIEGIDLDGVYFMRTPEDAIKLREAVDEGKIKRAVVVGGGF
                     IGLEVAENLTAQGVNTSVIDMAGQILPGFEPEIANYVDNYLADQRIITFTETGLEAIL
                     GDGKVEKIKTNNRTMKADAVVLSIGIRPNTAFLNGSGIELAPNRAIKVNEKLETNIKD
                     IYAVGDCAFVTNIITGKGAYAPMGSTANIEGRILAQNINGNNLNYKGAIGTAVVKLPG
                     INAGKTGLTEDAAKSEGYNVETVTTVMEDKAKYYPGASVFIIKMIADKVSKKLLGVQV
                     VGEGNIDKIVDMAVTAIGLDAKLEDIQNLDYAYAPPFSTAIHPFAATVNVLLNKINGN
                     LVSVTPREYKEGKADGYKIIDSSIVPKITGAPYVDISKAHEELPEFDKDEKLLLVCDK
                     GKRSYMLQQRLKDFGYTNTLVLEAGTMFNELK"
     gene            4346..>5294
                     /locus_tag="PKH42_08820"
     CDS             4346..>5294
                     /locus_tag="PKH42_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOA20576.1"
                     /translation="MANLKVTPAEEKTVKALGFLRNKGTDNFSARIITVNGKITAAQQ
                     KIIGEAAEKFGNGDVTFTSRLTIEVPGIPYEKITEFQDYIGKEGLVTGGTGSKVRPVV
                     ACKGTVCHHGLIDTFDVSEDIHNIFFEGYAPVKLPHKFKIGVGGCPNNCMKPSLNDLG
                     VIGQMVPNYDEELCMGCKKCSVEEACPIGAAKVVDGTLVIGDDCNNCGKCIGTCNFDA
                     IPDGDKGYKVFIGGMWGKRVNPGVPINKIFKTKEEVFEIIEKMILLYREQGQTGERVA
                     QTVNRIGFENVEAQLLSDEIFERKQEILEAELHMTGGATC"
BASE COUNT         1750 a          891 c          949 g         1704 t
ORIGIN      
        1 acagtatccg gaaaaagaat taaaaccgta tcggtcattg cacctacaag gctggatacc
       61 gcaacacctg ccaatataat tgtcattcgt gatgcccctg tttttcttgc cacgtaatat
      121 actgctatgg tggccaaaaa tgcacctaaa aatgcagcta ggggcattat gctaaggtta
      181 gcaggaaaaa aggctgtagc cagtgctgta aacagacctg ctcctgaatt taccccaatt
      241 atagtgggac ttgccaaggc attgtttaac acagactgta aaatcagtcc ggatatagct
      301 aatccgcttc ctgctattat tgctgacagg gtccttggta tccgtacata gttaataatt
      361 ttatattcag atgttagctg gccattaccg gatatggcat taaatatatc ggatatttcg
      421 atacttgaag aaccgacgga tatgctagta aaaataagta ctattaatag gattaacaga
      481 aagcttataa caaaaaagtt ataagctttt ttgtaattaa ttaaagattt cgggatagat
      541 aatttctgca aggtatctat agctttcacc ccattttgca tttggtttat agtggaacaa
      601 ttcttttggc agtatgatat acctgtcatt ttctacagca gataagctgt tccaggccgg
      661 atttgttcct atgccggttt ttagtgcatc tattgctttt tgttcatctc ccatagtcgt
      721 tacgaatatg aaatcagggt cttcttcaat aatctcttca atacttatat cctccaataa
      781 tgacgggtgc tgagctgcaa tgttgacggt tcctaaatta tccagcattg tacaagtcat
      841 attgtcatct ttcttagctt ttgctccgct tgaaaaggca cgaacaaata aaactttaag
      901 ttcttctttt tttgcacctt ctaccttata caatatatca tcaatatctt ttttgatttt
      961 aagtccgttt tcttcatata agtcttttct gcctgttata tcagtacata tatttaacat
     1021 gtttaaataa tcttcaaact cttccacttt aaaaaaagcg tgattaatat ttaattgttt
     1081 taaggtttct gcaattttca cctggttgtc aacatcggga gtcagtatta caaaatcagg
     1141 gttcagtgat aatattcctt ctaaatttgg ttcctttacg gtacctacaa tttgaatgtc
     1201 atttgttatt tccatgcgtc cttccttctg aacatcatcg gtaacacctg ctaagcttcc
     1261 acctgccaaa gcccaggttt ccgaatagga ccccaataaa gatactactc tttcaggttt
     1321 tctttctaaa acaacctctg tacccaatgc atctgaaaat atgtaataat tttcttcagc
     1381 atcttgatta tctatattta ttgcttccac attgtttgtt tctttgctgc aagctgctgt
     1441 aatcagcatc aatatcagcg ttaatataag aaacagattt ttctttttgc ttatcatact
     1501 accaccatct ttcctataaa aaaatatatt atcattgtta attaatacac atactttatg
     1561 tttataataa catctaagca gtttaattaa aaataataat atattatcac tgttataggt
     1621 aaaacctatt gattaattac tttcttaaaa tacccggatt aactattgct ttagagggta
     1681 tatacctata atcctttaca atatttatta gagactttat tgcatcagtc aataatttat
     1741 ctttatggta tactaggcta aagtgcctgt tccaagtgtt ttcttcattt tcaataacat
     1801 gaataagaga atcctttatt tcattttcta ccaaacaaat agatataact gacaggcaat
     1861 tgttatgaat aacttcctgc ttgatggatt gcggggtatt tccttcaaaa acaaccttta
     1921 tgggcattcc gttattaagc atgtattctt caaatagctc cctggttccg ctgcctttct
     1981 ctctcattgc aaaattttca tcacgaagct cgtctgtttt aattatcttt ttctgagcaa
     2041 aggggtgttt agtactgcat attaagacta aataatcttc taattcaggc ttaacaatta
     2101 agtcttgact tttaacttta ccttctacta ttgcaatatc caattcattg cttaataatt
     2161 tttcctctat cgtttcagtg tttcctacaa aagaatatat ttctacattt ggattttctt
     2221 ctatgcatct gtgtattaaa tcgcttaaaa tacaatctcc gacagatatt gtagccccaa
     2281 ctcttatttt atctttcttg ttcaaattat tcatcttact ctctaagctg tcgaattctt
     2341 ttaccacttc ttttgcataa aacaatagtt ttttacctgc ttcagtaata tacagccttt
     2401 tgctgagccg ttcaaataac aataccccgt aatgctcttc taattctctt atagcttgac
     2461 ttgccgtagg ttgtgaaata aaaagctttg aagctgctgc agtcatattg ccgcattgtg
     2521 ccacttcaat aaatattttt aaatgcctga tagtcatgag aactctcctt ttcaacatca
     2581 taggttttgc ctatgattat gataagattt tatcatttta ctaattataa agcaagtgtt
     2641 atattataca taaatgtctt gttaaataat acacaaagga aggtgcgttt atgaatatta
     2701 ttataattgg aggcgtagct gcaggtacta aggtagcggc aaagctaaag cgtgataatt
     2761 ataaccacaa tgtaaagata cttactaagg gtaaagatat atcttatgcc gggtgcgggc
     2821 tgccctatta tgtaggaaat gtaattgaaa aacaagaaga cttaatccaa aatacaccaa
     2881 agcaattttc tgaattgaca ggtgtagaag tattgactga agtagaagta actaaggtta
     2941 atcctgaaga aaaaaccgta gaagcattaa acttaattac aaatgaaact gctacatatt
     3001 catatgacat attagtaatt gcaaccggtg ctgaaccgat taaacctccg atagagggaa
     3061 ttgaccttga tggagtatac tttatgagaa ctcccgaaga tgccataaaa ttacgtgaag
     3121 cagttgatga aggcaaaata aagagagcag tagttgtcgg tggcggattt ataggattag
     3181 aggttgccga aaacttaacg gctcaaggcg taaatacttc ggtaattgat atggccggac
     3241 agattctccc cggatttgaa ccggaaattg caaattatgt tgataactat ttggctgacc
     3301 aacgaattat aacatttacc gaaaccggtt tagaagctat attgggcgat ggaaaagttg
     3361 aaaaaatcaa aacaaacaat cgtaccatga aagcagatgc agtagtttta tcaataggta
     3421 taaggcctaa tacagctttt ttaaacggta gcggtattga attagcacca aatagagcta
     3481 tcaaggttaa tgaaaaactt gaaaccaata tcaaagatat ttatgcagta ggtgattgtg
     3541 cttttgttac aaacattatt accggtaaag gtgcttatgc tcccatgggc tctactgcca
     3601 atattgaagg aagaatatta gcacaaaaca taaatggaaa caatttaaat tacaaaggtg
     3661 ctattggaac agcagttgtt aaacttccgg gaattaatgc cggaaaaacc ggtttgacgg
     3721 aagacgctgc taagtcagag ggttataatg tagaaactgt tactaccgta atggaagaca
     3781 aagcaaaata ttatcccggt gcttcagttt ttatcataaa gatgattgca gataaagttt
     3841 caaagaaact gttgggtgta caagttgtcg gtgaaggcaa tattgacaag atagtagata
     3901 tggctgttac ggcaatcgga ttagatgcaa agttagaaga tattcaaaat ctcgactatg
     3961 cttatgctcc cccattttca acagcaatac atccttttgc ggctaccgta aatgttttat
     4021 taaataaaat aaacggtaat ttggtttctg ttacaccaag agaatataaa gagggcaagg
     4081 ctgacggata taagattata gattcatcaa tagttcctaa aattacaggt gctccttatg
     4141 tagatatttc aaaggcacat gaagaattgc ctgaatttga taaagatgaa aaactattgt
     4201 tagtatgtga taaaggcaag agatcctata tgctgcaaca gagattaaaa gatttcggat
     4261 atacaaatac cttagtactt gaagccggta ctatgtttaa tgaattaaaa taaataatat
     4321 agatttagaa gaggagaata aaattatggc aaatttaaag gttacacctg cagaagaaaa
     4381 gactgttaaa gctcttggtt ttttgagaaa taagggtaca gacaattttt ctgctcgaat
     4441 tattactgtt aacggaaaga ttacagcagc tcaacaaaaa ataataggag aagctgcaga
     4501 gaaatttgga aacggagatg taacatttac cagcaggttg acaattgaag taccaggcat
     4561 accttatgag aaaatcactg aattccaaga ttatatcggt aaagaaggat tagttacagg
     4621 aggtacaggc tctaaggttc gtccggtagt agcttgtaag ggtacagttt gtcatcatgg
     4681 attaattgat acttttgatg tatcagaaga tatacacaat atattttttg aaggttatgc
     4741 accggtaaaa ttaccgcata aattcaaaat aggtgtaggc ggatgtccaa ataactgtat
     4801 gaagccttca ttaaatgacc tcggcgtaat cgggcagatg gtgcctaact atgatgaaga
     4861 actttgcatg ggttgcaaga aatgctctgt agaagaagca tgtccaattg gtgctgcaaa
     4921 agttgttgat ggtacattag taatcggtga cgattgcaac aactgcggaa aatgtatagg
     4981 aacttgtaat ttcgatgcca ttccggatgg tgataaagga tataaagtat ttatcggcgg
     5041 aatgtggggt aaaagagtaa atcccggagt tcccataaac aaaatattta aaacaaagga
     5101 agaagtattc gaaattattg aaaaaatgat actcctttac agggagcaag gacaaacagg
     5161 tgaaagagtt gctcaaacag taaacagaat cggatttgaa aatgttgaag ctcaattgtt
     5221 atctgatgaa atatttgaaa gaaaacaaga aatattagaa gctgaacttc atatgacagg
     5281 cggagctacc tgct
//
LOCUS       DAOPUE010000125         5269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_15681_length_5269_cov_11.355006, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000125 DAOPUE010000000
VERSION     DAOPUE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15681_length_5269_cov_11.355006"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKH42_08825"
     CDS             <1..410
                     /locus_tag="PKH42_08825"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOA20577.1"
                     /translation="ADSINEENLDEVVEYIKSLSEKLSSVIAVTGAIDIVSDSKKTYI
                     IRNGHPMMGKISGTGCMLSSIIGAYCGANKDNIVDATAAAVCIIGLSGELAYEKILKN
                     DEGTSMLRSHLIDFMSKIDDKILEEGAKFEIRQ"
     gene            394..1032
                     /gene="thiE"
                     /locus_tag="PKH42_08830"
     CDS             394..1032
                     /gene="thiE"
                     /locus_tag="PKH42_08830"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOA20578.1"
                     /translation="MKLDSKSLLLYAITDRAWLKGKTVAEAVEEAILGGATCIQLREK
                     NLSDEEFLKTAMDVKAVTDKYNIPFIINDNVDIAVRVGADGAHIGQDDEEIKSAREKL
                     GADKIIGLSAATVEEAVQAEQSGADYIGVGAVFNTLTKLDANTVSFETLKEICNTVKI
                     PVVAIGGISKDNALQLAGTGIEGISVVSAIFAQNDIKTAASELLELAKKIIE"
     gene            1043..1825
                     /gene="thiD"
                     /locus_tag="PKH42_08835"
     CDS             1043..1825
                     /gene="thiD"
                     /locus_tag="PKH42_08835"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOA20579.1"
                     /translation="MKKVLTIAGSDSSGGAGIQADLKTMTSHKIYGMSVITALTAQNT
                     TGVYGVFESSPEFVAQQMDAVFTDIYPDAIKIGMVSNKEIISVIADKLIEYKAGKIVL
                     DPVMVATSGGKLFKDDAVEMLINKLIPLATVITPNIQEAQVLSGIEIESKEDMQRAAV
                     KISKNYSGYILIKGGHLTLSSDDLLYSNGEFIWYEQPKINNLNTHGTGCTLSSAIACN
                     LAEGNDVKTSILNAKKYITGAIKSNLNIGKGRGPLNHCWNLG"
     gene            complement(1896..3005)
                     /locus_tag="PKH42_08840"
     CDS             complement(1896..3005)
                     /locus_tag="PKH42_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA20580.1"
                     /translation="MTQMIYTVQPGDTLYSIARLYGSTIQAIVDANNILNPNLIYPGS
                     VILIPVEEEYLETPPGSLIYTVQPGDSLYIISLLFKVSIQRILELNDIPDPSLIYPGM
                     KIILPPEALNPFQPIVPGIIRYTVLPGDTIYKIAARFGTTAQSIINANPGLDPRRLIP
                     GTVITITIPENAVAIYRGNPNRRMVALTFDATYGDNQTYELLEILRNNNIKATFFLSG
                     IWLINYPDLARAIAAEGHEIGNHSLTHPHMPLITMQEVTNQIVRTEALIRNITGQDPY
                     LFRPPYGEYTQAILNQLASLGYVTILWTIDSLDWQNPGAAAIVSRVVNNAEPGAIILL
                     HQSAPDTLEGLQSMITQLRQQGYDFGTVTQVISPL"
     gene            3176..4342
                     /locus_tag="PKH42_08845"
     CDS             3176..4342
                     /locus_tag="PKH42_08845"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOA20581.1"
                     /translation="MNNFTVPFSFTLPTKIVYGPGCISSLIDELHLNNGNRPIIVTDK
                     GVRNAGILDKITFLLERNNTDYIVYDGVEPNPKDVNVEEGARIAREFGADSIIAIGGG
                     SPIDCAKSVGVLLAHKEDKIKKYDGKTAATKPLPLLITIPTTSGTGSELTFSSVITDT
                     LNNYKMTVKSQYTAAKVAICDPELTLSLPAHITASTGMDALTHAIEAYTATCAEPISD
                     AMALYATELIYNNLVTAFNDGVNLEARSAMLLGSMLAGIAFSHSDVASVHCIAESLGG
                     VYDLPHGVCNAVFLPHVMEYNMEYSVKRYARIAKAMGLSFENEREGAKKAVEAIKKLA
                     VDVKLPLFSSLKVDINDFEKIAEMSVKNISTESNPRPMTKEDYLTVIENAYKHK"
     gene            complement(4400..5206)
                     /gene="zupT"
                     /locus_tag="PKH42_08850"
     CDS             complement(4400..5206)
                     /gene="zupT"
                     /locus_tag="PKH42_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOA20582.1"
                     /translation="MNNNILLAFGLTLIAGLSTGIGSAIAFFAKKTNTKFLAISLGFS
                     AGVMIYVSMIEIFAKAKDSLVAELGLKAGSWAAVASFFAGMLLIAIIDNVLPNEENPH
                     HLHNVEELNETKVHIEDNLMRTGIFTALAVGIHNFPEGLAAFISALQEPSIAIPITVA
                     IAIHNIPEGISISAPIYYATGDRKKAFKYSFLSGLAEPIGALIGYLILMPFLNDLVFG
                     IVFGVVAGIMVYISLDELLPSAQKYGDHHLSIGGLISGMAVMAVSLLLFI"
BASE COUNT         1724 a          930 c         1100 g         1515 t
ORIGIN      
        1 ttgctgattc aattaatgaa gaaaatttag atgaagttgt tgaatatatt aagtcattat
       61 cagaaaagct ttcttcggtg attgccgtaa ccggtgcaat agatattgtt tcagacagta
      121 agaaaacata tattattcga aacggacatc caatgatggg taaaatatcc ggtacaggtt
      181 gtatgctttc gtctataatc ggtgcatact gcggtgctaa caaggacaat attgtcgatg
      241 ccacagcagc tgctgtatgt atcatcggat taagcggtga attagcttat gagaaaattt
      301 taaagaatga tgaaggaaca tcaatgctaa ggtcacattt aatagatttt atgagcaaaa
      361 ttgatgataa aatattggag gaaggtgcga aatttgaaat tagacagtaa atcactgcta
      421 ttatatgcaa ttactgatag agcctggtta aagggaaaaa cagttgccga agccgtggaa
      481 gaggcaatac tggggggagc aacttgtatt cagctaaggg aaaaaaattt aagcgatgaa
      541 gaatttttaa aaactgccat ggatgttaag gcagttactg acaaatacaa tattccattt
      601 ataatcaatg ataatgtaga tatagcagtt agagtcggtg ctgacggagc acacatcggc
      661 caagacgacg aagaaataaa atcagccaga gaaaaattgg gagctgataa aataatcggt
      721 ctttctgccg ctacagtgga agaagctgtt caagccgagc agagcggtgc agattatata
      781 ggagtgggtg cagtttttaa tactttaaca aaacttgatg caaatacggt atcatttgaa
      841 accttaaagg aaatatgtaa taccgtgaaa atacctgtgg ttgcaatcgg aggaatatcc
      901 aaagataatg cacttcaact tgcaggtacg ggcatagagg gaatatctgt tgtatcggct
      961 atttttgcac aaaatgatat taaaactgct gcatcagaac ttttagagtt agcaaaaaaa
     1021 ataatagaat agaggaagta atatgaaaaa ggtattaaca atagccggtt ctgactcaag
     1081 cggaggagca ggaatacagg ctgacttaaa aacaatgacc agccacaaaa tatacggtat
     1141 gagtgttata acagcactga cggctcaaaa tacaacagga gtatacggag tattcgaatc
     1201 atcgcctgaa tttgttgctc agcagatgga tgcggttttt acggatattt atcccgatgc
     1261 aataaaaatc ggaatggttt cgaataaaga aattatatct gttattgcag acaagctgat
     1321 tgaatacaaa gcaggaaaaa tagtgcttga cccagtaatg gttgccacga gcggaggcaa
     1381 actttttaag gacgatgcag tagaaatgct tataaataag ctgattcctt tagcaaccgt
     1441 aataacaccc aacatacagg aagctcaggt gcttagcggg attgaaattg agagcaaaga
     1501 agatatgcaa agagcagcgg taaaaatatc caaaaattat tcgggctata ttcttatcaa
     1561 gggaggacac ttaactctta gcagcgatga tttattatat tcaaatggtg aatttatatg
     1621 gtatgagcag cccaagataa ataatctaaa tacacatgga acaggctgca cgctttcatc
     1681 ggcgatagcg tgcaatttag cagagggcaa tgatgttaaa acaagcatat taaatgcaaa
     1741 aaaatatata acaggcgcaa taaaatctaa tttaaatata ggaaaaggaa gaggaccttt
     1801 aaatcactgc tggaatttag gttaaaaact tatagcactt acaaaagtaa gcggacgtgg
     1861 ttggtaatga ttatgacaac cccgtcctct tgtgattata gcggacttat tacttgtgta
     1921 actgttccga aatcgtatcc ttgctgtctt aattgtgtta tcattgattg cagcccttca
     1981 agtgtgtcgg gtgccgattg atgcagcagg ataattgctc cgggctctgc attatttaca
     2041 actctcgata ctattgcagc agctcccgga ttttgccaat ctaaggagtc aatcgtccac
     2101 aggattgtta catagccaag tgaagccaat tggtttagaa ttgcttgcgt atattctcca
     2161 taaggcggtc tgaacaaata tgggtcttga cccgtgatat tcctgataag agcttctgtt
     2221 cgaacaatct ggtttgtgac ttcctgcatt gttataagcg gcatatgagg gtgggtaagg
     2281 ctgtggtttc ctatttcatg accttcagct gctatagccc ttgctaaatc aggataattt
     2341 atcaaccaga ttccggacag gaaaaatgtt gccttaatat tattgtttct taatatttca
     2401 agcaattcat aagtctggtt atcaccgtaa gtagcatcaa atgttaaagc aaccattctt
     2461 ctgtttggat ttcctctgta tatggcaaca gcattttcag gaattgtaat tgtaattacc
     2521 gttcccggaa taagtcttcg agggtccaga ccgggattag catttataat agactgagct
     2581 gttgttccaa accttgcagc aattttatat atagtatccc cgggcagtac ggtatatctg
     2641 attatgccgg gcactatagg ttgaaatgga ttaagagctt ctgggggcag tataattttc
     2701 attcccggat aaatcaatga agggtcggga atgtcgttta attctaaaat tctttgaatg
     2761 cttaccttga ataaaagaga aataatatag aggctatcgc cgggctgtac ggtataaata
     2821 aggcttccgg gcggggtttc taagtattcc tcttcaacag gtatcaatat aacagagccg
     2881 ggatatataa ggtttggatt taaaatatta tttgcatcta caattgcttg tattgtactt
     2941 ccatacaggc gagcaatact ataaagcgtg tcaccgggtt gtaccgtata tatcatttga
     3001 gtcataataa tcctcctatt cttttctata taatattccg aatattatac aaagtgatag
     3061 tatgaatatg acactacccc gtcctttgtc atttaatagt gttgaaatat ttcggacatg
     3121 taatataata aagatatgta actacggata tcgttaatta ccaaggagga tttgaatgaa
     3181 taattttact gtgccattta gttttacttt gccgacaaag attgtgtatg gaccgggatg
     3241 catctcttct ttaatcgacg aattgcactt aaacaacgga aatagaccga ttattgttac
     3301 cgataaaggt gttagaaatg caggcatact ggataagata acttttttgc tggagcgaaa
     3361 caatacagat tacattgttt atgatggtgt tgagccaaat ccaaaagatg tgaatgtaga
     3421 agaaggagca agaatagcaa gagaattcgg tgcagattca attattgcca ttggaggagg
     3481 aagcccgata gattgcgcta aatctgttgg agtgctctta gctcacaagg aagataaaat
     3541 caagaaatac gatggcaaaa cagctgctac aaagcccttg cctctgttaa taaccatccc
     3601 gacaacttca ggcaccggca gcgagctgac attttcttcc gttattacgg atacactgaa
     3661 taattataaa atgactgtta aaagtcaata tactgcagcg aaggttgcaa tttgcgaccc
     3721 ggagcttacc ttatcactcc ctgcacatat taccgcttcc actggcatgg atgctctaac
     3781 ccatgcaatt gaagcttata ctgcaacttg tgcggagccc ataagtgacg ccatggcttt
     3841 gtatgcaaca gaattaattt acaataatct tgttactgct tttaatgacg gagtcaatct
     3901 tgaagcaaga agcgcaatgc tgcttggcag catgcttgca ggcatagcat tcagccattc
     3961 cgatgtggct tctgtccatt gcatagctga gtctttggga ggagtatatg accttcctca
     4021 cggagtctgc aatgctgtat ttctgcctca tgttatggag tataacatgg aatattccgt
     4081 aaaaagatat gcacgaatag ccaaagctat gggtctaagc tttgaaaatg aaagagaggg
     4141 agcaaaaaag gctgttgaag cgataaaaaa attagcagtt gatgttaaac tgcccttgtt
     4201 ttcaagctta aaggtagaca taaatgattt tgagaaaatt gcagaaatgt cagttaaaaa
     4261 tatttcaacc gaaagcaacc cgagacccat gacaaaagaa gactatttga ctgtcattga
     4321 aaacgcctac aaacataaat gatgacacta cccccgtccc cttgtcatga aaaacccttc
     4381 gcgaagcgaa gggtttaggt tatatgaaca ataagaggct tactgccatt acagccatgc
     4441 cggatatcag cccgcctatt gaaaggtgat ggtctccgta tttctgtgcc gaaggaagca
     4501 attcatccaa tgatatatat accattattc cggcaactac accgaagact atgccaaaca
     4561 ccaaatcatt taggaaaggc attaatatta aataacctat aagtgcaccg atgggctcag
     4621 caagtcctga aagaaaagaa tatttgaaag ctttttttct gtcccctgta gcataataaa
     4681 tgggagccga tattgatatt ccttcaggga tattatgaat agctattgct acggttatgg
     4741 gtatagctat cgaaggctct tgaagtgcag aaataaatgc tgcaagacct tcgggaaagt
     4801 tatgtattcc aactgccagt gccgtaaata taccagttct cataaggtta tcttctatat
     4861 gaacttttgt ttcatttaat tcttcaacat tgtgcaggtg gtgcgggttt tcttcattgg
     4921 gcagaacatt gtcgataatt gctataagta acatgccggc aaaaaatgat gcaactgctg
     4981 cccatgaacc tgcctttaag ccaagctctg ctaccaggct atcctttgct ttagcgaata
     5041 tttcaatcat ggaaacataa atcattaccc ctgccgaaaa gcccaggctg attgctaaaa
     5101 atttggtgtt tgtttttttt gcaaaaaatg cgatagcact gccaattccc gtgctaagtc
     5161 ctgctatcag cgttaatcca aatgctaata aaatattatt gttcatttgc acctcctaaa
     5221 actattatat acccaattaa ataaaaatgt aaactaatta tagcaatta
//
LOCUS       DAOPUE010000126         5182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16067_length_5182_cov_9.150185, whole genome shotgun sequence.
ACCESSION   DAOPUE010000126 DAOPUE010000000
VERSION     DAOPUE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16067_length_5182_cov_9.150185"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="PKH42_08855"
     CDS             complement(<1..2264)
                     /locus_tag="PKH42_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOA20583.1"
                     /translation="MNEIQLNYIVQAILGGASGYITNDYAINMLFKEYTPLKLGGVIK
                     KTRNEFIENLSSLVENDIINKEKLLEIFESPEFKFKFEKLTCDFYENCLYETVGNGKF
                     SDIDGFDSSLKGLDNFVIEILNNNLESLIGLIADNFDAEYFLTNSQLEKIADSIYNYI
                     KDVIENTDAVENILKNIYENNKELNLSHIFGSNMQTTDNLINKLTEIVADNAEHSELF
                     AGFNAALNEALKVFYDKQIKDVIKFDKEIINSFISYIEKNDIILKLCRSLFSYGRSID
                     KSLYSIIDPAFESSLKAYIENNLPFVTEKLVSYVQKNSILIDRIIEDTLDEVVSESEG
                     FRKRMLHVIKNTYFNNLSKKYSIVDKIISYIRQFTEPEKLSIDISKNIIERLDNITVS
                     DIISETENNFNLYKVYEIVAGFLNKNAMAIYENIENYISKLRIKDIFPPIDLNAGKIL
                     SSEQAQKYIKNKSQNYVKAVLSKQINELLENNSDIFIKSTADYLKVKFIDNEISIKNF
                     LSKKIKDFKIDNNYLKSKELTNFIRLELYDKYKEEASKLKDFDLSLALDKLNSIENIS
                     KNSSETFRKYIISNTDIILKGSVKGVVTDNLNKLSDDELVKFANDFVGRELKPIMFFG
                     GVLGVIAGLILAAFQNSPLDPANVNIANMATYAFVGFITNVIAINMIFKPYREIKPLS
                     KIPFFRNFSLGYIIKNQKNFAKSTAHYIDTNLLSKKSINEMFEKHKDSIKQSFIKNIA
                     ENDYSTLSSLLVKN"
     assembly_gap    2339..2438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2656..3339
                     /locus_tag="PKH42_08860"
     CDS             2656..3339
                     /locus_tag="PKH42_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA20584.1"
                     /translation="MNEKILVVDDEENLLNVIKDYLLRESYEVYTANRGNKAIELFRK
                     IEPDFIILDLMLPDISGEEICKLIRKESNVPILMLTAKSSEDDKVTGLYIGADDYLTK
                     PFSPRELVGRVRAILRRTKGTAADVLEFNDKDLYIDIPKHIVKKAGESIKLTPNEFKI
                     LSVLAQNPHKVFTRSHLVSLAFGYDFEGFDRTVDTHIKNLRQKIEDNVKEPKYIVTVY
                     GVGYKFEGV"
     gene            3341..4708
                     /locus_tag="PKH42_08865"
     CDS             3341..4708
                     /locus_tag="PKH42_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA20585.1"
                     /translation="MVKISKQLRKYFITISILSIAFITITSNISINFFFSGYIRDSRS
                     RDDLKVLKYVESVFSEYEEINDNSSMNLMHYAFSEDVVIQIKDTNSSIMFNSSAFGTI
                     IGITDEYSKNEEKFSFRSYPFIIDDEQVGTIDIGRPKSIIANIEDEKFLITINSIFAA
                     ASLLTLIIALRSSAHLSKKFLNPIYRIQENAKLIEQGKYKNINEVETNTSELHELSVS
                     VKELAERLHHQETLKRRMTTDIAHELRTPLAAVQSHIEAFMDGVWEPDYERLSVIHGE
                     VIRLTKLISELSNLSIVEGDEIKLKLSTVDLSEILNDIVDSYEPMFSDKNISVNKNIQ
                     NDVNMMGDIDYLKRIFINILSNAFKYTNENGTVSVSLEQIKNQIRITVKDTGIGIPKE
                     DLKHIFERFYRSDLSRNRQTGGTGIGLTITKALVEAHGGTIKIDSEVGKGTNVIVEFV
                     NLNHH"
     gene            4870..5016
                     /locus_tag="PKH42_08870"
     CDS             4870..5016
                     /locus_tag="PKH42_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HOA20586.1"
                     /translation="MFKFIKNWLDRLAKENEKNFGTDRLDCCGLNKPKNTNTQSSNSN
                     TDRK"
BASE COUNT         1790 a          729 c          803 g         1760 t
ORIGIN      
        1 ctatttttaa caagcaggct gcttagtgtt gaatagtcat tttcggcaat gttttttata
       61 aagctttgtt taatgctgtc cttatgcttt tcaaacattt catttatgct ttttttgctt
      121 aataggtttg tgtcaatata atgggcagta ctctttgcaa agtttttctg gtttttaatt
      181 atatagccta gcgagaaatt gcggaaaaaa ggaattttag acaaaggctt aatttctctg
      241 tagggcttga atatcatatt tattgctatt acatttgtaa taaagcctac aaaagcatag
      301 gttgccatgt tggctatatt cacatttgct gggtcaaggg gtgaattctg aaatgcagcc
      361 aaaattaagc ctgcaataac tcccaatacg ccgccaaaaa acattatggg ttttaactcc
      421 cgacctacaa aatcattggc aaatttaaca agctcatcat cactcagctt atttaaatta
      481 tctgtcacaa cacccttaac agagcccttt aaaatgatat ccgtattgct tattatgtat
      541 ttgcgaaagg tttccgagct gttttttgat atattttcta tagaatttaa tttatccaaa
      601 gctaaagata aatcaaaatc tttcaattta gaggcttctt ccttatattt atcatataat
      661 tctaaacgga taaaatttgt aagctccttg ctttttaaat aattattgtc tattttaaaa
      721 tcctttattt ttttggatag aaagttctta attgaaattt cgttatcaat aaattttact
      781 tttaaataat cagctgtact tttaataaat atatctgaat tattttcaag caattcattt
      841 atttgcttgg ataacacagc tttaacataa ttttgtgatt tattttttat atatttttgt
      901 gcctgttctg aagataaaat tttacctgca ttcaaatcaa tcggtggaaa aatatctttt
      961 attcgaagtt ttgaaatata attctcaata ttttcataaa tagccatggc atttttgttt
     1021 aaaaagcctg caacaatttc ataaacctta taaaggttaa agttgttttc tgtttctgag
     1081 ataatgtcgc ttacggttat attatccaaa cgctcaatta tatttttgct tatatctatg
     1141 ctcaactttt caggctctgt aaactgcctt atatatgaaa taattttgtc aacaatgctg
     1201 tattttttgc ttaaattatt aaaataggtg tttttaatca catgcagcat tctttttcta
     1261 aagccttccg actcactaac tacttcgtct aaagtatctt ctataatcct gtcaataaga
     1321 atactgtttt tttgaacgta gctgactaat ttttcagtta caaagggaag attattttcg
     1381 atatatgctt taagactgct ttcaaaggca gggtctatta tggaatacaa gcttttgtct
     1441 atgcttctcc cgtaagaaaa taaagagcgg cataatttaa gaataatatc atttttttct
     1501 atgtatgaaa taaaggaatt tataatttcc ttatcaaact taatcacatc tttaatttgc
     1561 ttgtcataaa aaaccttcaa tgcttcattt aaagcagcat taaatcctgc aaataattca
     1621 gaatgttcag cattatctgc tacaatttct gtaagcttat ttattaaatt atctgtcgtc
     1681 tgcatatttg agccaaatat atgagaaaga ttcaattctt tattgttttc ataaatattt
     1741 tttaaaatat tttcaactgc atcagtattt tcaataacat ctttaatata attataaatg
     1801 gaatctgcta ttttttccaa ctgagaattt gtcagaaagt attctgcatc aaaattatct
     1861 gcaattaatc caattaaact ttccaaatta ttattaagaa tttctataac aaaattatct
     1921 aaacccttca atgaggagtc aaacccgtca atatccgaaa atttgccatt gcccacagtt
     1981 tcataaagac agttttcata gaaatcacag gtaagttttt caaatttgaa cttaaactca
     2041 gggctttcaa aaatttcaag aagcttttct ttatttataa tgtcattttc aaccagtgag
     2101 ctcaaatttt ctataaattc attacgcgtt tttttaataa cgccgcctaa tttcaaggga
     2161 gtatattctt taaaaagcat attaattgca tagtcatttg taatataacc ggaggctcct
     2221 cctaatattg cctgtactat gtaattcagc tgtatttcat tcattgaagt ctacctttca
     2281 ttccatacct ttttagggat tatagcattt aatattttaa attgcaaaaa acttttttnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc actgtttagg ggggacaagt
     2461 attataacaa tcacttttgt ttgaaaacta tacagattat acaagtaaat tatgtagaaa
     2521 taatgaagaa aaacaaattt attaattttc ttcataaacc acatgtttta atattggatt
     2581 taaagagtat aatatgagca atatactaaa tttaaggtat tgctaacatt aataacagaa
     2641 aattaaaggt gaaaaatgaa cgaaaaaatc ttagttgttg atgatgagga aaatttacta
     2701 aatgtaataa aggattacct tttaagagag tcctatgaag tttatacggc taatcgcggg
     2761 aataaagcta tcgaattatt tcgtaaaata gaaccggact ttattatact tgatttgatg
     2821 cttcctgata tttcgggaga ggaaatatgt aaacttataa gaaaggaatc aaatgttcct
     2881 attttgatgc ttactgctaa aagcagcgaa gatgataagg ttaccggttt atacatcggt
     2941 gcagatgatt atttaaccaa acctttcagt ccaagggaat tagtcggcag ggtaagggca
     3001 attcttagaa gaacaaaggg cactgcagct gatgttttag aatttaatga taaagatttg
     3061 tatattgata tacctaaaca tatagtcaag aaagcaggcg aatcaataaa actgacacct
     3121 aatgaattta aaatattatc agtattagca caaaatcctc ataaagtatt taccaggagc
     3181 catttagtca gtctggcttt cggatatgat tttgaaggat ttgatcgcac tgttgataca
     3241 cacataaaaa atttgcgtca aaaaatagaa gataacgtaa aggaacctaa atatattgta
     3301 actgtctacg gtgtcggata taagtttgaa ggtgtataag atggttaaaa taagcaagca
     3361 attaaggaag tactttatca caatttcaat attatcaatc gcatttatta caattacttc
     3421 taatataagc ataaactttt tcttctccgg ctatataagg gattcaagaa gcagagatga
     3481 tttaaaagtt cttaagtatg tggaaagtgt attcagtgaa tacgaagaaa taaatgataa
     3541 ttcttcaatg aatttaatgc attatgcttt cagcgaggat gttgttatac agattaagga
     3601 tacgaatagc agtataatgt ttaacagcag cgcatttggt acaatcatag gaattacaga
     3661 cgaatacagc aaaaatgaag aaaaattttc gtttagaagc tatccgttta ttattgatga
     3721 tgaacaagtt ggaactattg atattggacg acctaaaagc ataatagcca atattgagga
     3781 tgaaaaattt ttaattacca taaacagtat attcgcagcg gcttcattgc tgacattaat
     3841 aatagcattg cgttcaagtg ctcatttatc aaagaagttt ttaaatccaa tatatagaat
     3901 acaagaaaat gctaagctga tagaacaagg aaaatataaa aatataaatg aagtggaaac
     3961 aaatacatcc gaattgcatg aattgtccgt atctgtaaaa gagcttgccg aaaggttgca
     4021 ccatcaggaa acattaaagc gaaggatgac tacggatata gcccatgagt taagaacacc
     4081 cttagctgcc gttcaaagcc atatcgaggc ctttatggac ggggtatggg agcctgatta
     4141 tgagcggctt tctgttattc acggcgaggt aataagatta actaaattaa tcagtgaatt
     4201 atccaattta tcaatagtag agggcgatga aataaaatta aaattaagca ctgttgattt
     4261 atcggaaatt ttaaatgata ttgtagacag ttatgagcct atgttttcag ataaaaatat
     4321 ttctgtaaat aaaaacattc aaaatgatgt aaatatgatg ggagatatag attatttaaa
     4381 acgaattttt attaatatcc tttcaaatgc atttaagtat actaatgaaa atggaactgt
     4441 cagtgtttca ttagagcaaa taaaaaatca gataagaata acagtaaaag acacgggtat
     4501 tggtatccct aaggaagatt taaaacatat ttttgaaagg ttttacagga gcgatttatc
     4561 aaggaatcgt caaaccggag gaaccggcat tgggcttaca attacaaagg cattggttga
     4621 agctcacggt ggtaccataa aaatagacag cgaggttgga aaaggaacaa atgtaattgt
     4681 tgaatttgtt aatctgaatc atcattaata ttagatcctt cgcttgtgct caggatgaca
     4741 taatagaata tatatatctt cataacttct tcacaaagta ttgttaatat tcatcataca
     4801 ccccccaccg gggtatggca tttttatgct ttatatctat atagacagct aaaaaaggag
     4861 gtaatattta tgtttaaatt cataaaaaat tggttagaca gattagctaa ggagaatgaa
     4921 aagaattttg gaaccgacag gctcgattgc tgtggtttaa ataaacctaa aaacacaaat
     4981 actcaatctt ctaatagcaa tactgatagg aaatgaaatg caatgattgg gaaagagttt
     5041 ggtaggtatt atctgctatg cagattttaa gcgccatgga tttctttaaa attatgacag
     5101 attttacaac ttgaatgata taaaaataag gttctatacg ttgcagtcaa catttagatt
     5161 attggctgct ttttcttatt ga
//
LOCUS       DAOPUE010000127         5137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16255_length_5137_cov_10.398268, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000127 DAOPUE010000000
VERSION     DAOPUE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16255_length_5137_cov_10.398268"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PKH42_08875"
     CDS             complement(<1..1252)
                     /locus_tag="PKH42_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOA20587.1"
                     /translation="MRGYTERTKRLREISTRTQPSVSLERALIETSVYEKYYGTVEIP
                     VLRALNFKNLMEKRKLYIGEDELIVGEKGEGPQVAPTFPELCCHTVEDMTVMNDRKYI
                     NFKVKEEDKKLQQEKIIPYWEKRSIRHKILESMTQEWKDCYAAGMYTEFMEQRAPGHT
                     VADGKIYEKGFLDFKNEIEEEIEKLDFMNDPDAYDKKAQLEGMAISCDAIIIYGQRYA
                     QYARELASKESNPKRKEELLWIANNCDVVPAHKPETFAQALQMYWFVHIGITTELNTW
                     DSFSPGRLDQYIYPFYKKEKEEGTIDYHKARELLECFWIKFNNQPAPPKVGITLKESG
                     TYTDFANINTGGINPYTGEDGVNEISYMILDVMDELKLLQPSSNVQISVKTPDEFLKR
                     AVEISRKGWGQPAFYNTEELIQELL"
     gene            complement(1254..2150)
                     /locus_tag="PKH42_08880"
     CDS             complement(1254..2150)
                     /locus_tag="PKH42_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOA20588.1"
                     /translation="MKASIFNIQKFSIHDGPGIRTTVFFKGCPLQCIWCHNPESQNLG
                     KEILYDKNKCTLCGSCIKICQNNAIELKDNDLEINMDKCTFCGDCTVCCINSAKQIAG
                     KEYTVDEVMKEVLKDRVFYKNSKGGVTLSGGEPLIYAAFVEELLMELKKENIHTAVDT
                     CGAVDFKVLERISKYTDLFLYDIKSMDEEKHILYTGVSNKNIINNLINLSKIHNNINL
                     RLPIIEGINADENHIFEILKLIKNTNIKKINLLPYHDIAMHKYEKLGRKYYEYMKRPA
                     DEKLKRYIDIIEKEGYRVKIGG"
     gene            complement(2140..2784)
                     /locus_tag="PKH42_08885"
     CDS             complement(2140..2784)
                     /locus_tag="PKH42_08885"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOA20589.1"
                     /translation="MNLKNIKDKLKNIKPKPIDFDVSFAVLVPIIEIDGELNLIFEVR
                     SNSITQPGEISFPGGRIEEGESPRKAAIRETSEELLLNKENIEIISELNYASSKSGAF
                     VFTFLGLIKDVDPDEIEYSIDEVSEVFFVPLSFFLENRPEKYYMNYYPKADKDFPYHM
                     VNNGEDYNWGNIRYPVYFYKYYNYIIWGLTAKITYSLIKELTNSDVKKRNNNES"
     gene            complement(2785..3336)
                     /locus_tag="PKH42_08890"
     CDS             complement(2785..3336)
                     /locus_tag="PKH42_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOA20590.1"
                     /translation="MYKEFLETFILVFVAEMGDKTQLMLMTLAAKYKIIDVLLGIFLG
                     VLLNHGTAVFIGSCISNLIDEYLLHVFAGVVFIIFGIITIVFEDEDENKKQYGLGPVI
                     TTALTFFLGEMGDKTQLTCMTLSMDAYYPSAVLAGSVTAMLAIGLAGIIIGTSLTKFI
                     PSYIIKTISGLIFIIFGVVRMII"
     gene            3441..4538
                     /locus_tag="PKH42_08895"
     CDS             3441..4538
                     /locus_tag="PKH42_08895"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOA20591.1"
                     /translation="MDKNILITTAPFGNGHKMVATAIKNAFIKKGYNNVVVVDLFTET
                     HPKITEAIKKAYIRSYDFGKAYSKLYYGSEKLTDKKVVELYRNFGFSKLKDISEVFKP
                     DVIINTFPILASLKLKNEQGENIPVFNIVTDYYAHKLWISKEIDKFYIATYELQEEFK
                     KMNIPPEKIVVSGIPIRDAFEEIDNISKLHAKYKFNRRKRIILINSGAFGVLKDIKKV
                     CTELCKNKNIQVAVVCGNNIYQKTKLESLRLRNLKVFGFVEDIHELYKIACCMITKPG
                     GITLTEALSVQLPLIIIKPVPGQEKENARYFEKKGAAIIANNTYQIINSAIDLINNPE
                     LLSAMRKNMKKMYNKSSSDRIVDDVLGSLEE"
     gene            complement(4596..5054)
                     /locus_tag="PKH42_08900"
     CDS             complement(4596..5054)
                     /locus_tag="PKH42_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOA20592.1"
                     /translation="MRIIIDGDACPQKVREICEKAAKDFGVGLIIVSDIDHYIVSDFE
                     VIVVEQGRDSVDYKIVQIFQQGDILITQDYGLASLLLGKASAIIHTAGFFINKNNIDS
                     LLQSRYISDKIRKQGGKSKGPSKRRKEQDENFKKCLYKVLREYMSNKKGR"
BASE COUNT         1568 a          983 c          771 g         1815 t
ORIGIN      
        1 tcaacaactc ctgaatcaat tcctccgtat tgtaaaatgc cggctgaccc cagccttttc
       61 ttgaaatttc aacagccctt tttaaaaatt catcgggagt tttaacactt atctggacat
      121 ttgagcttgg ttggagaagc ttcaactcat ccattacgtc aagtatcata taagatattt
      181 catttacacc gtcttctccc gtataggggt ttattccccc cgtgttaata tttgcaaagt
      241 ccgtataagt tccgctttcc ttcaatgtta ttcctacctt ggggggagca ggctggttgt
      301 taaacttaat ccaaaagcat tccaacaact cccttgcctt atggtaatca attgtaccct
      361 cttccttttc ctttttatag aagggataaa tatattgatc tagtcttcca ggcgaaaagg
      421 agtcccaagt attaagctct gttgttatgc ctatatgcac aaaccaatac atctgcagag
      481 cttgtgcaaa agtttcaggt ttatgagcag gcactacgtc acagttattt gcaatccaca
      541 ataattcttc ttttcttttc ggatttgact ctttagaagc taactctctt gcatactgtg
      601 catatctttg accgtaaatt attattgcat cacatgaaat ggccatacct tccagctgtg
      661 cctttttgtc gtaggcatca gggtcattca tgaaatcaag tttttcaatt tcttcttcaa
      721 tttcattctt aaaatccaga aatccttttt cgtaaatttt tccgtctgca actgtatgcc
      781 cgggagctct ttgctccata aactctgtgt acatacctgc agcataacaa tccttccatt
      841 cctgtgtcat tgattcaagg attttatgtc ttatggagcg cttttcccaa tatggaataa
      901 ttttctcctg ctgcagtttt ttatcttctt ctttaacctt aaagtttata tattttctgt
      961 cattcatcac ggtcatatct tcgacagtgt ggcagcaaag ctccggaaat gtgggggcta
     1021 cttgaggccc ttcgcctttt tctccgacta tgagttcatc ttcccctata taaagtttcc
     1081 ttttttccat caaattttta aaatttaacg ccctcaatac aggaatttcc actgtaccgt
     1141 agtatttttc ataaactgaa gtctctatta aagctctctc taaactaacc gatggttgtg
     1201 ttcttgtgct gatttctctt aatctcttgg ttctttcagt gtatcctctc atgtcagcct
     1261 cctattttga ctctgtaacc ttctttttca attatatcta tatatcgttt caatttttca
     1321 tctgcaggtc ttttcatgta ctcataatac tttcgaccca gtttttcata tttatgcatt
     1381 gctatatcgt ggtaaggcag caaattaatt tttttaatat ttgtattctt aattaatttt
     1441 aatatttcga aaatgtgatt ttcatcggca ttgattcctt caattatagg taatctcaaa
     1501 tttatgttgt tatgaatttt agacagatta attaaattat taattatatt tttgtttgaa
     1561 actcccgtat atagtatatg cttctcttca tccatggatt ttatgtcata tagaaataag
     1621 tccgtatatt tagatatcct ttccaacact ttaaagtcaa ccgctccgca agtatcaact
     1681 gctgtatgaa tattttcttt ttttaactcc attaacaatt cctcgacaaa ggctgcatat
     1741 ataagaggct ctcctccgga caatgttaca ccgccctttg agtttttata aaatactctg
     1801 tcttttagta cttctttcat tacttcatca acggtatatt cttttccggc aatctgtttt
     1861 gcggagttta tgcagcaaac agtgcaatcc ccgcaaaaag tacacttgtc catatttatt
     1921 tcaaggtcat tgtcttttaa ttctatagca ttgttttgac atatcttaat acaagatccg
     1981 cataatgtgc atttattttt gtcgtataaa atttctttac ctaaattttg gctttccgga
     2041 ttatggcacc atatgcactg taaggggcat cctttaaaga ataccgtcgt tcttattccg
     2101 ggtccgtcat gtatagaaaa tttctgaatg ttgaatatac tagctttcat tattattcct
     2161 ctttttgacg tcactgtttg taagctcttt aataaggctg taagttattt tagctgtcaa
     2221 cccccatatt atataattgt agtacttgta aaaataaaca ggatatcgaa tattccccca
     2281 attgtaatct tccccgttgt taaccatatg ataaggaaaa tccttgtcgg cctttggata
     2341 ataattcata taatattttt caggccgatt ttctaaaaaa aatgacaagg gtacaaagaa
     2401 aacttccgaa acttcatcaa tgctgtattc tatttcatcc gggtcgacat ccttaatcag
     2461 ccctaaaaat gtaaatacaa aagccccgct tttgcttgaa gcataattca gctctgaaat
     2521 tatttctata ttttccttat ttaacaatag ctcttctgaa gtttctctta ttgcagcttt
     2581 ccttgggctt tcaccttctt caattcttcc tcccggaaac gaaatttctc ccggttgagt
     2641 aatggaattt gatctaacct cgaatattaa gtttaattct ccgtcaattt caattatggg
     2701 aaccaatact gcaaagctta catcaaaatc tatgggtttc ggttttatat ttttaagctt
     2761 atcttttatg ttttttaaat tcatttatat tatcatcctt actactccga atattataaa
     2821 tatcagtccg gatattgttt ttattatata ggaaggaata aatttagtca atgaagttcc
     2881 gataataatt cctgccaaac ctattgcaag cattgctgtt acggagcctg ccaatactgc
     2941 tgaagggtag tatgcatcca tggataaggt catgcatgta agctgagttt tgtctcccat
     3001 ttctccgaga aaaaaagtaa gagcagtggt aataacagga cccaggccgt attgcttttt
     3061 gttttcgtcc tcgtcttcaa atacaatagt aataattcca aaaattatga aaacaacacc
     3121 tgcaaataca tgaagaagat attcatctat taaattagag atacagctgc ctataaagac
     3181 cgcagttcca tgatttaata aaacaccgag aaaaattcct aaaagcacat caattatttt
     3241 atacttggct gccaaggtca taagcatcag ctgggtttta tctcccattt cagcaacaaa
     3301 gaccaagatg aaggtctcca gaaattcttt atacattttc cctccctgac aatatactct
     3361 ctttctttta tccgagttat atattataat attaaaagcg acgtacaaac agaacaacat
     3421 taatcatttc gggaggtatt atggataaaa atatattgat aaccaccgcc ccctttggaa
     3481 acggccataa aatggttgca actgcaataa aaaatgcatt tattaaaaag gggtataaca
     3541 atgtggttgt tgtagatttg tttactgaaa cacatcccaa aatcaccgaa gctataaaaa
     3601 aggcatatat cagaagctat gattttggaa aagcttactc aaaactttat tatggctcag
     3661 aaaaactaac ggataagaag gtagttgaat tatacagaaa tttcggattt tcaaaattaa
     3721 aggatatatc tgaagtattt aaacctgatg ttataattaa tactttcccc attttggcat
     3781 ctttaaagct gaagaatgaa caaggagaaa atatacctgt tttcaatata gtaaccgact
     3841 attatgctca taaactttgg ataagcaagg aaattgacaa gttttatatt gcgacatacg
     3901 aattgcagga agaattcaaa aaaatgaata tacctccgga aaaaatagtc gtatccggaa
     3961 tacccatcag ggatgctttt gaagaaattg acaatatttc gaaactccat gccaaatata
     4021 aatttaacag acgaaaaaga ataattctta taaactcagg cgcatttggt gtattaaagg
     4081 atattaaaaa agtatgtacg gagttgtgca aaaataaaaa tattcaggta gcagtagttt
     4141 gcggcaacaa tatatatcag aaaacaaaat tggaatcctt gaggctcaga aatctaaaag
     4201 tgttcgggtt tgttgaagat attcatgaac tatacaaaat tgcatgctgt atgattacca
     4261 aaccgggtgg aattacattg acggaagcat tatcggtaca gctgcctctt attataatca
     4321 agcctgtacc cggtcaggaa aaagaaaatg caagatattt tgaaaaaaaa ggtgcagcaa
     4381 taatagccaa caatacttat caaattataa acagcgctat tgatttaata aataatcctg
     4441 aacttctgtc cgcaatgcgc aaaaacatga agaaaatgta caataaatct tcatcagaca
     4501 gaattgttga tgatgtgtta gggagcttag aggaataaag taagggacat tactttatgt
     4561 cccccttcct atgatatgat ttttttcgtt tagaattacc tgcccttttt atttgacata
     4621 tattctctta aaactttata caggcatttt ttaaagtttt catcctgttc ttttcttctt
     4681 tttgacggtc cctttgattt tccgccttgt tttcttattt tatcacttat atatctggat
     4741 tgaagcaggc tgtcaatatt attcttattt ataaaaaacc ctgctgtatg aatgattgct
     4801 gatgctttcc ccaaaagaag gcttgcaagt ccgtagtctt gagttattaa tatatctcct
     4861 tgctgaaata tttgaactat tttataatca acggaatctc ttccctgctc aacaacaata
     4921 acttcaaaat cactgacaat ataatggtca atgtctgaaa caattatcaa gcccactcca
     4981 aaatccttgg ctgctttttc acatatttct cttaccttct gcgggcatgc atctccatca
     5041 attataattc gcatgaaaaa ctcctttttt aattaagtta tgatattata tcttttttgt
     5101 taacagttgc aaataatttt ataaatccgt cattgca
//
LOCUS       DAOPUE010000128         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16465_length_5090_cov_9.106455, whole genome shotgun sequence.
ACCESSION   DAOPUE010000128 DAOPUE010000000
VERSION     DAOPUE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16465_length_5090_cov_9.106455"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1358)
                     /locus_tag="PKH42_08905"
     CDS             complement(36..1358)
                     /locus_tag="PKH42_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA20593.1"
                     /translation="MKKILLIQPSPYDKNHKPIKKNKLYFVGLAMPLLAAMMPEDWEA
                     EIVLEVIEDIPFDTDAELIGISTMGHGVIRSIDIARKFREMGKTVILGGYMASIMAEE
                     ALKHCDSVVVGNAELVWDELLKDYEEGKLKKIYKNNLESGFFSTPMPRFDLIINKRIG
                     NFLPVQAGRGCPNTCSFCSVACLYKGHYIKKPLEEVVRDIKQVKELGFKKFLLLDDNI
                     FSDREYLDKLLIEIKKLNMQWMSQCDIRIGRENELLKKLADSGCITLSFGLESISKES
                     LKGMNKSWANPLEYTELIQNIHSHGIDVSTEMVVGGEGDTLQSIRDTKRFIENNKITV
                     PRFYILTPIPGTKFFEDMTKAGRIIKEDIYSFDGTEAVHRPKNMSPEELTNAYWELYD
                     SLFTFSSILKRNILRKEFLINPSKYLFYTIVNLFYRYQIKNRITPNIF"
     gene            complement(1358..2719)
                     /locus_tag="PKH42_08910"
     CDS             complement(1358..2719)
                     /locus_tag="PKH42_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOA20594.1"
                     /translation="MKVLLVRPRPHKETIGLQHVMICEPLELEYLISNIPDDIKGETD
                     AEIIDMILEKGSFEEVVYKKKPAFIVFTGYITHVGIIKQMAETAKKIIPSVKTGVGGV
                     HAEVVPEDFESEYIDFVYYKNGIDNFNITLRGLINKKSINEIKEEINIEDKKVIFDYK
                     YPDRNSVKKYRKKYYYMFHNPCALIKTSFGCPYNCSFCFCKEITQGQYFTREMDDIIK
                     ELSSIEEEEVYIVDDDFLYDRNKLESFIAGLKLNNIKKKFLVYGRADFIAQNKDIIAK
                     LKEVGLQAVIVGIESVRAGDLKDYKKGTTVEINEKCIQILQELDIELYATLIIPLDFK
                     KEDFSELVSWLRKLNIKFVNLQPLTPLPGTEIFESYKGSLLADRQRFEVWDMAHVVLK
                     PRYMSIRAFYAEILKAYYKVVMRPKHLGYLIKKYGLKANFKMLAGSTFVSFQYLQKII
                     RSN"
     gene            complement(2716..3333)
                     /locus_tag="PKH42_08915"
     CDS             complement(2716..3333)
                     /locus_tag="PKH42_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOA20595.1"
                     /translation="MFNIWNFWADKYDKLWVQKYSLKPTRNYIVKALSKYIKNDGIKV
                     LDLGCGTGELIFELTNKFNNLEVTGIDFSEKMIEISKKRNPSAKHLQMDASELYILDS
                     KYDIIVCTHSMPYYKEPKNVFKHLYNLLSDEGKLLIGFASGDSIYDKFILGFVKLTTG
                     KANYPSDNEFRAMIFPFFEVENLKIIRKKFFMPRIAVYTLKKVKL"
     gene            complement(3345..4613)
                     /locus_tag="PKH42_08920"
     CDS             complement(3345..4613)
                     /locus_tag="PKH42_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20596.1"
                     /translation="MIVFLEKVRVKTIFSIIDPVKVEPLELCYLKSVLNNMNIESYIV
                     DELFDFSPPKDITPDIVVLTGYNTAENEIIRMSEVYKNKYPPVKIIAGGVHVQENAEN
                     FRVNTIDYVFHSPSLNTFRLLINKIINNDGKPLNRGVDTNYKRGWHLGEKESIYEIEN
                     IKPDRDFFYKAGTKLKYLDKKNVALIKGSIGCPYNCSFCYCKLLNNKHFIAADYEKMI
                     EEMESINADYYWVVDDVLFSTRNDALKFIEIIEKRNSNLNIIGYLRADFICREKDLLK
                     KLRNAGIREVITGFEATGNEELNEYKKTTDAADYPLAISLLKENNIELTALFMVQPSY
                     GLKEFKNLKKFIKTNKIDLYTISIMTPLKKTKLYEETEELLIDKRPDKYDFLHLVIKP
                     KLPKFIFYTLFYSLHIRLLKSKRVRKFLFN"
     gene            complement(4618..>5090)
                     /locus_tag="PKH42_08925"
     CDS             complement(4618..>5090)
                     /locus_tag="PKH42_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20597.1"
                     /translation="KNNEFSEKHYDLYLSIMNRTDNPLETLPIEFFKNYDSEIYEFLN
                     KGTLNVIGFVQLKEFDGRLCFLFGGFNKDDVKKYDLYYNMLLKIIEAGIEKKAHIIEF
                     GQTAEESKLKIGCKEVYKYLYVHHSNSILNFIIQKLLPIMSYRPYRTVHHVFKE"
BASE COUNT         1517 a          958 c          687 g         1928 t
ORIGIN      
        1 cctatctttt gctgttttaa aatggggtta gggcattaaa atatattcgg cgtaattcta
       61 ttttttatct ggtatctgta aaacaaattt acaattgtat aaaacaaata tttgcttgga
      121 tttattaaaa attcttttct taatatattc ctttttaaaa tcgagctgaa tgtaaacaat
      181 gaatcataca attcccagta tgcatttgtt aattcttcgg gactcatatt cttaggccta
      241 tgcactgcct ccgtaccatc aaaggaataa atgtcttctt tgataatcct tcctgctttc
      301 gtcatatcct caaaaaattt agttccggga ataggagtta atatatagaa ccgcggcaca
      361 gtgattttgt tattttcaat aaatcttttt gtatccctta tggattgcag tgtgtctccc
      421 tcgccaccta caaccatttc tgttgaaacg tctataccgt gagaatgtat gttttgtata
      481 agctcagtat attccaaagg atttgcccag gatttgttca tcccctttaa actttccttt
      541 gaaatacttt caagtccaaa acttaatgtt atacatccgc tgtcagccag tttttttaac
      601 agttcattct ctctgcctat acgaatatca cactggctca tccactgcat atttagtttt
      661 ttaatttcaa ttaaaagttt gtccaaatat tctctgtcag aaaaaatatt gtcatccaac
      721 aaaagaaact ttttaaatcc taactctttt acctgcttaa tatctctaac tacctcttca
      781 agaggctttt taatataatg acctttatac aggcatgcaa cagaacagaa gctgcatgtg
      841 ttgggacagc cccttcctgc ctgaacaggc agaaaattac cgattctttt atttattata
      901 aggtcaaatc gaggcattgg agttgaaaaa aagccgcttt ccaaattatt cttatatatt
      961 ttcttaagtt ttccttcttc gtaatctttt aaaagctcat cccaaaccaa ttccgcattc
     1021 cccactacaa cactgtcgca gtgtttcaat gcttcctcag ccatgatgct tgccatgtag
     1081 cctcccagta ttacggtctt tcccatttcg cgaaatttcc ttgcgatatc aattgagcgg
     1141 ataaccccgt gtcccatggt gctgattccg attaattcgg catccgtatc aaaaggaata
     1201 tcttcaatta cctctaatac aatttcagct tcccagtctt caggcatcat tgctgccagt
     1261 aaaggcatgg caagtccaac aaaatacaat ttgtttttct tgataggttt atggtttttg
     1321 tcataggggc ttggttgtat caataatatt tttttcatta attactcctg ataatttttt
     1381 gtaaatactg aaaacttaca aaggttgagc ctgcaagcat tttaaaattt gctttcaagc
     1441 cgtatttctt gattaaatat cctaaatgtt tcggtctcat tacaaccttg taatatgctt
     1501 ttagaatttc tgcataaaaa gctctgatac tcatatatct tggttttaaa acaacatgtg
     1561 ccatatccca cacttcaaat ctctgtctgt ctgccaagag tgagccttta taagactcaa
     1621 atatttcagt tccgggaaga ggtgtaagcg gctgaaggtt aacaaatttt atatttaatt
     1681 tcctgagcca acttaccaac tcactgaaat cttctttttt aaaatcaagg ggaattatca
     1741 atgttgcata tagttcaata tctaattcct gtaatatttg aatgcatttt tcgtttattt
     1801 caaccgttgt tcccttcttg taatccttta aatcacctgc tcttactgat tctattccga
     1861 ctattacggc ctgcaagccg acttccttaa gttttgctat aatatctttg ttttgtgcaa
     1921 taaaatcagc tcttccgtaa accaaaaatt tcttttttat attatttaac tttagtcctg
     1981 caatgaaact ttcaagtttg tttctatcat ataaaaaatc atcatctact atatatactt
     2041 cctcttcttc aattgaggat aattctttta taatatcgtc catttcacgg gtaaaatatt
     2101 gaccttgggt tatttcttta caaaaacaaa acgagcagtt gtatgggcag ccaaaggaag
     2161 ttttaatcaa ggcgcaaggg ttgtgaaaca tatagtagta tttttttcta tattttttta
     2221 ctgaatttct gtcaggatat ttgtaatcaa atatgacttt tttatcttca atatttattt
     2281 cttcttttat ttcattaata ctttttttat ttattaaacc tcttaatgtt atattaaaat
     2341 tatcaattcc gtttttataa taaacaaaat ctatatattc cgattcaaag tcctcaggta
     2401 caacttctgc atgaactccc cctacccccg tcttaaccga aggtataatt tttttcgcag
     2461 tttctgccat ttgctttata atcccgacat gggttatata gccggtgaaa actatgaatg
     2521 caggtttctt cttatataca acctcttcaa aacttccttt ttcaagtatc atatctataa
     2581 tttcagcatc tgtttcacct ttgatatcat caggaatatt cgaaataaga tattcaagct
     2641 ctaaaggctc gcatatcatt acatgctgca aacctatcgt ttctttatga ggtcttggtc
     2701 ttacgagtaa tactttcata atttcacctt cttaagagtg tagactgcta ttcgaggcat
     2761 aaaaaatttc ttcctgatta tttttaaatt ttccacttcg aaaaagggaa atatcattgc
     2821 tctgaattca ttatccgagg gataattggc cttgccggtg gtaagcttaa caaagcctaa
     2881 tatgaatttg tcatatatgc tgtcaccgct tgcaaaccca ataagtaatt ttccttcatc
     2941 gcttaataaa ttatacaggt gtttgaaaac attctttggc tctttgtagt aaggcattga
     3001 atgggtgcac actattatgt catacttgct atccaaaata tataactcag atgcatccat
     3061 ttgtaaatgt ttggctgagg gatttctttt ttttgatatt tcaatcattt tttccgaaaa
     3121 atcaattccc gtaacttcaa gattattaaa tttatttgtt aattcaaaaa ttaattctcc
     3181 agtgccgcac cctaaatcaa gtacttttat tccatcattt tttatatatt ttgaaagagc
     3241 tttaactata taatttctcg tgggttttaa agaatatttt tgcacccaca gcttatcata
     3301 tttatcagcc caaaaattcc atatattaaa catatgctct cctcttaatt gaacagaaat
     3361 tttcttactc tttttgattt tagaagtctt atgtgaaggc tgtaaaacaa tgtataaaaa
     3421 ataaatttcg gcagtttcgg ttttattacc aagtgcaaaa aatcatactt atcaggtctc
     3481 ttatcaatca gtaactcttc cgtttcttca taaagctttg ttttctttag aggagtcata
     3541 attgaaatgg tgtacaaatc tattttattt gtttttataa acttttttaa attcttaaat
     3601 tcttttaggc catagctggg ctgcaccata aaaagggctg ttaattcaat attgttttct
     3661 tttaataagg aaattgccaa gggatagtcc gcagcatcgg ttgttttttt gtattcatta
     3721 agctcctcgt ttcctgttgc ttcaaagcct gtaataacct ctcgaatgcc tgcatttcta
     3781 agcttcttta acaagtcctt ttccctgcat atgaaatctg cccttaaata tcctatgata
     3841 tttaagttgg aatttctttt ttcaataatt tcaataaatt ttaaagcatc gtttcttgta
     3901 gaaaacaata catcatcaac tacccaatag taatcggcat taatactttc catttcttca
     3961 atcatttttt cataatctgc agctatgaaa tgcttgttgt taagaagttt gcagtaacaa
     4021 aatgagcaat tgtacggaca tcctatgctg ccttttataa gagcaacatt ttttttatct
     4081 aaatatttta gttttgttcc ggctttataa aaaaaatccc tgtccggttt aatattctca
     4141 atttcgtaaa tactctcttt ttctcccaga tgccagcctc tcttataatt tgtatcaaca
     4201 ccacggttta aaggtttacc gtcattattg atgattttat taatcaagag cctaaatgta
     4261 tttaagcttg gtgaatgaaa aacatagtct attgtattta ccctgaaatt ttctgcattt
     4321 tcctgcacat gaactccccc tgctattatt tttaccggtg gatatttatt cttgtatacc
     4381 tcagacatcc ttataatttc attttctgcc gtattgtaac ccgtaagcac tacaatatca
     4441 ggagtaatgt cttttggagg actaaaatca aacagttcgt ctacaatata gctttcaata
     4501 ttcatattat ttaaaaccga cttcaaatag cataattcca agggctcaac ctttacaggg
     4561 tcaatgattg aaaatatggt tttaaccctt actttttcta aaaaaactat catagtttca
     4621 ttccttaaaa acatgatgga ctgtcctgta tggtctgtat gacataatag gaagcaattt
     4681 ttgtattata aaatttaaaa tagaattgct gtgatgaaca taaagatatt tatatacttc
     4741 cttgcagccg atttttaatt tactttcttc tgctgtctgc ccaaattcaa taatgtgagc
     4801 tttcttttca atgcctgctt cgataatctt taaaagcata ttgtaatata aatcatattt
     4861 ttttacatcg tctttgttaa atcccccaaa taaaaagcac agcctgccgt caaattcctt
     4921 cagttgtaca aatccaataa catttagggt gcctttattc aaaaactcat aaatttcaga
     4981 atcataattt ttaaaaaatt caatcggtag agtttccaaa ggattatcgg ttctgttcat
     5041 aatgcttaaa tataaatcgt aatgtttttc actgaattca ttgtttttaa
//
LOCUS       DAOPUE010000129         5025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16751_length_5025_cov_13.151107, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000129 DAOPUE010000000
VERSION     DAOPUE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16751_length_5025_cov_13.151107"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKH42_08930"
     CDS             complement(<1..987)
                     /locus_tag="PKH42_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HOA20598.1"
                     /translation="MQEKINRVAPGILLSASVAVIAMFLSSLIPGDIIGATVMALLVG
                     MALNPLFSKYEQFNKGVSFTGKMILRLGIILMGVNMNFAEVLDVGKYSLFVMIFTMGT
                     AFGAGNLVGKLFGMNWKLTNLLSVSTAICGGSAVAAVGPVIKAKDEDVAYAISSTFIF
                     DVLTVVVIPWIGMALGMSNMGYGLWVGTAVNDTSSVVAAGYAFSEFAGNTAVIVKLTR
                     TLFIIPYVLIFSFITERIEAKTVSGQVHKPVNIKKIFPYFIIMFLVVVLFRSTGIIPE
                     TIVPGLSKSSKFFMVMALSAIGLKTSYSDIKNIGYKPMILGFIVDTLVVFVSI"
     gene            1098..1997
                     /locus_tag="PKH42_08935"
     CDS             1098..1997
                     /locus_tag="PKH42_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOA20599.1"
                     /translation="MLDNKLQTFLTLCETQNYSQAAKKLNITQPAVSQHIQYLENYYG
                     VLLISNKGKNFELTDEGKALREYGGTLKANSERILPLLQSIKNKSKPLNFGATLTIGE
                     YTVPPILSQIFKEDPEINISMVIENTVVLQKMLWEGKIDFALLEGHFNQEQFEFKLFS
                     NETFIGVCSPENEIVNKVSDLEELLEQNLILRETGSGTRDIFEQALYNQNLSIMSFKR
                     RIEINSMNAIIELCHQNIGITFMYREAAKKEISLDYLKEIPIRNFNISHPFCFVYLKN
                     NPNKSQLEFWFEKFISLRNPLGQ"
     gene            complement(2143..2274)
                     /locus_tag="PKH42_08940"
                     /pseudo
     CDS             complement(2143..2274)
                     /locus_tag="PKH42_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
     assembly_gap    2331..2430
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2431..3034)
                     /gene="uppS"
                     /locus_tag="PKH42_08945"
     CDS             complement(<2431..3034)
                     /gene="uppS"
                     /locus_tag="PKH42_08945"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOA20600.1"
                     /translation="MRIPRHIGLIPDGNRRWAQDKGLQKQDGYDFGLDPGLAALKLSV
                     EYGIDEITYYGFTTDNCKRPKIQIESFVKACVDAVNIIAKENVSLLVLGNTKSPMFPK
                     ELLKYTKRTNIGNGGIKVNFLVNYGWDWDISNINVTSSNRKTIMDCLHSKDISRIDLI
                     IRWGGRKRLSGFLPVQSVYSDIYTIDDLWPDFTPEQFHNAI"
     gene            3272..3667
                     /gene="tsaA"
                     /locus_tag="PKH42_08950"
     CDS             3272..3667
                     /gene="tsaA"
                     /locus_tag="PKH42_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOA20601.1"
                     /translation="MEIKMKPIGYIRTNYKYDSEIPRQSINNMNEKGTIEILDEFKDG
                     IKDIEIGSYVIVLFYFHKSDYWHLISEKRDNKGVYSTRSPRRPNGIGMSIVKIINSEN
                     STLEFLGVDMIDGSPVLDIKPYMDFSPII"
     gene            3908..4855
                     /gene="arcC"
                     /locus_tag="PKH42_08955"
     CDS             3908..4855
                     /gene="arcC"
                     /locus_tag="PKH42_08955"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOA20602.1"
                     /translation="MNRIPRVVIALGGNALEDKSMPSTAEAQLEVVKKTSEYIADISC
                     SNYEIAIVHGNGPQVGRILLASEAAKDITPAMPFDVCGAMSQGYIGYHIQQSMNLALA
                     KRNRNVQVATVVTQVVVDKDDPAFQNPTKPIGPFYSEEEAKKLAEEKGYTVKEDAGRG
                     WRRVVASPIPKRIVEIDTIKNLWDTTITVACGGGGIPVIENEDGSLKGVEAVIDKDFA
                     AERLAEDMDSDMLLILTEVEKVSINYKKPNQEDLSVLTLEQAEKYIAEGHFAPGSMLP
                     KVMAAVKFAGSRPGRKAIITSLYKALDALEGSTGTVIEG"
BASE COUNT         1748 a          818 c          893 g         1466 t
ORIGIN      
        1 tatggaaacg aaaaccacca atgtatcaac aataaatcca agaatcattg gtttatatcc
       61 aatatttttt atatcagagt agcttgtttt taaaccgata gcggacaatg ccataaccat
      121 gaagaatttt gatgattttg acagccccgg cactattgtc tcagggatta taccggtgct
      181 gcgaaacaac acaacaacaa gaaacattat aataaagtaa gggaaaattt tcttgatgtt
      241 aacaggttta tgtacttgtc ctgaaactgt ttttgcctca attctttctg ttataaaaga
      301 aaatattaat acgtagggaa taataaacag tgttcttgta agttttacta ttacggctgt
      361 atttcctgca aattctgaaa aagcatatcc tgccgctaca actgacgagg tatcattaac
      421 agccgttcct acccacaacc catatcccat attagacatt ccaagagcca tgccaatcca
      481 tggtattact acaactgtta gaacatcgaa aataaaggtt gacgaaattg catatgctac
      541 atcctcatct ttggctttaa taaccggtcc aaccgcagca acagcagagc cgccgcatat
      601 agctgtagaa actgacaaaa gatttgtaag cttccagttc attccaaaga gttttccgac
      661 taaattccct gcaccaaaag cagttcccat agtaaaaatc ataacaaaaa gtgagtattt
      721 gccaacatct aaaacctctg caaagttcat attgacaccc ataagtatta ttcctaaacg
      781 aagaatcatt ttccccgtaa agcttactcc tttgttaaat tgctcatatt tactaaataa
      841 agggttcagt gccataccta ccaatagtgc cataacagta gcccctatga tgtcgccggg
      901 aattaagctg cttaaaaaca ttgctataac ggcaacactt gcactcagta aaattccagg
      961 tgccacccta tttatttttt cttgcataaa aatacctcca cttaatttta aataatatgt
     1021 tgattatatc attgaaattt tataaaatat aattataata tattatagtc atataagcta
     1081 aacttatgga ggctaacatg ttagataata agttgcagac atttctcaca ttatgtgaaa
     1141 ctcaaaatta ttcgcaagca gctaaaaagc taaatataac acaaccggct gtttcacaac
     1201 atatacagta tcttgagaat tattacgggg tattattaat atctaataaa gggaaaaatt
     1261 ttgaacttac cgacgaaggc aaagctttgc gtgaatatgg tggaacactt aaggcgaact
     1321 ctgagaggat tttaccttta ttacaaagca ttaaaaataa atcaaaaccg ttaaactttg
     1381 gagcaacttt gaccatagga gaatatacgg ttccgcctat attatcacaa atattcaaag
     1441 aagatcctga aattaatatt tccatggtta tagaaaatac agtagtgctc caaaagatgc
     1501 tgtgggaagg gaaaatagac tttgctttat tggaagggca ttttaatcag gaacaatttg
     1561 aatttaaact cttttccaat gaaacattta ttggggtatg ttctcccgaa aatgagattg
     1621 taaataaggt ttctgactta gaggaattgc ttgagcaaaa tctcatactt cgggagacag
     1681 gaagcggtac ccgtgatata ttcgaacaag cactgtataa tcaaaattta agcatcatga
     1741 gttttaaaag aagaattgaa ataaacagta tgaatgctat aatagagcta tgtcatcaaa
     1801 atatcggaat tacatttatg tacagagaag ctgcaaaaaa agaaatttct ttagattatc
     1861 tcaaagaaat tccaataaga aattttaata taagtcatcc tttttgtttt gtttatttaa
     1921 aaaataatcc caataaatca caattagagt tttggtttga aaaattcata tcattacgca
     1981 acccattggg gcagtaggtc aatcgcaatg agttcccaat ttatgctacg tcaggagcaa
     2041 ataaattagt gaacgagact tcggacgtat cattactgtc ccattaatta tatttaaaac
     2101 agccgtatat cagtgaatgg aattgatttt atataacccg gcctaccctc ctaatgttac
     2161 atcctgattg tcgtaccatt taattgcatt atgaaattgt tcaggagtga aatcaggcca
     2221 caaatcatca attgtataaa tgtctgaata aacagattgt accggcaaga atccgtaatt
     2281 gcattatgaa attgttcagg agtgaaatca ggccacaaat catcaattgt nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taattgcatt atgaaattgt tcaggagtga
     2461 aatcaggcca caaatcatca attgtataaa tgtctgaata aacagattgt accggcaaga
     2521 atccggacag tctttttctt cctccccatc gtattattaa atcaattctt gatatatctt
     2581 tggaatgaag acaatccata atggtttttc ggttagagct agttacatta atgtttgata
     2641 tatcccaatc ccatccgtaa ttaacaagga agttaacctt tatgccacca tttcctatat
     2701 tagttctttt agtatatttt aataattctt ttggaaacat tggtgattta gtattgccaa
     2761 gaacaagcag cgaaacattt tcttttgcaa ttatattgac ggcatctaca caagccttaa
     2821 caaatgattc aatttgaatt tttggcctct tgcagttatc agtagtaaaa ccgtaatatg
     2881 taatttcatc aattccatat tctacagata attttaaagc tgcaagtccg gggtcaagcc
     2941 caaaatcata accatcctgc ttttgcagtc ctttatcttg agcccatctt ctgtttccat
     3001 caggtataag ccctatatgt cttggtattc gcatatacac acacctttct tatgatgatg
     3061 gtattatttg caataatttc acaattattc tgcacataat atttgctaat aacagaccgt
     3121 tttatagtac actacacatc atgatgtata ataagtgtcg aataaacaaa aatttcatta
     3181 tgttgaaaga tgtaacaatt tggtatataa ttaatgcaaa tgtttaccga cctttaagaa
     3241 aaaaggattg gaaaatttga gggacaataa aatggaaata aaaatgaagc caatcggcta
     3301 tatcaggaca aattacaaat atgattctga aatcccgaga caatcaatca ataatatgaa
     3361 tgaaaaaggt accattgaaa tattggatga atttaaagac gggatcaagg atattgaaat
     3421 aggttcatat gtaattgtct tgttttattt tcataaatct gattactggc atttgatttc
     3481 tgaaaaacgg gataataagg gtgtctattc aacaaggtca cctcgcagac ctaatggcat
     3541 aggaatgtcc attgtgaaga taattaattc ggaaaattcc acattggaat ttcttggtgt
     3601 agatatgata gacggcagtc cggttttgga tattaagcca tatatggact ttagtccaat
     3661 aatatagtaa atatgaacaa ttactgggta aggaggggtg aaattatgtg cgaattagtg
     3721 aataaggtgt ttggtggttg aaaggtttat tgggcatgga ctttaatgag gattgcgaat
     3781 caaaaattaa taaataatac ttgaattaat attatatcat ttatataata tatttgctta
     3841 caaaaaacaa gataaaaccg caacagtatc acaagaaatt aaaaaattaa atcataagag
     3901 gtaaataatg aacaggatac caagagtagt aattgcattg ggaggcaatg cgttagaaga
     3961 taagagcatg ccctcaacag cagaagccca gttggaagta gttaagaaaa caagtgaata
     4021 tatagctgac attagttgct caaattatga aatagcgatt gtgcatggaa atggaccaca
     4081 ggttggaaga atattgttag cttcagaagc tgccaaggat ataacccctg caatgccttt
     4141 tgacgtatgc ggggcaatga gccagggcta tatcggttat cacatacagc agagtatgaa
     4201 tttagcactt gcaaaaagaa acaggaatgt acaggtggca acagtggtaa cacaggtggt
     4261 tgttgataag gatgatccgg catttcaaaa tcctaccaaa cccataggac ctttctattc
     4321 tgaagaagaa gctaaaaaac ttgctgaaga aaaaggatat acagttaaag aagatgcagg
     4381 aagaggatgg agaagagttg tagcatcacc gataccaaag aggatagtcg aaattgatac
     4441 cattaagaac ttatgggata ctaccataac agtagcctgc ggcggcggag gaattcctgt
     4501 aatcgagaac gaagacgggt cccttaaggg ggttgaagct gttatcgaca aggatttcgc
     4561 tgctgaaaga cttgcagagg acatggacag tgatatgctt ctgatattga cagaagttga
     4621 aaaggtgtcc ataaactaca agaagccaaa ccaggaagat ttatcggtat tgactttgga
     4681 gcaggctgaa aaatacatag ctgaaggtca ctttgctcca ggcagcatgc ttccaaaggt
     4741 gatggcggca gtgaaatttg caggttcaag accgggcaga aaagcaatta tcacatccct
     4801 ttacaaagca ttggatgcac tggaagggtc aaccggaaca gtaatagaag gataggaaac
     4861 agaatgtgaa atggctgttg ctttgtgatg cttcccatta ctaatacaac agctattttt
     4921 gattagtaat cattttttat tactatacct aaactttaat ttggagttac aaaaatgtat
     4981 aattatgagt ctgtattaaa tggcattgaa gatgatatta aggat
//
LOCUS       DAOPUE010000130         4991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16908_length_4991_cov_11.181524, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000130 DAOPUE010000000
VERSION     DAOPUE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16908_length_4991_cov_11.181524"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PKH42_08960"
     CDS             complement(<1..779)
                     /locus_tag="PKH42_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20603.1"
                     /translation="MQINYNITGEKRKSLVNAISQELNASSKYLGAPTFAYEVAGYNI
                     DKNGVLMGNDNYELVEDLQGLYDFKAVTEEYDSPIPESEPVPEGLIIPYEAALGGRVS
                     PYRDFEEPSNYGAKEEVEETSSIDNLELSIPMDGHSGTTLKNIVNMLSSKQHLIMKSI
                     GIEEKLMDGKFAEDLSLKTTTTLDEFKVAVEEIGTERCKGITFDFENNIYTYNIPSDN
                     LAYEKISSFAVLAARINEFAKEQKRTSYKVTQDDNPKFALRT"
     gene            complement(900..1823)
                     /locus_tag="PKH42_08965"
     CDS             complement(900..1823)
                     /locus_tag="PKH42_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HOA20604.1"
                     /translation="MASAEFLKGRFGIEVEMTGITRTKAARTVANHLRGTIERCYGSY
                     DTHEVTATDGRVWKIVSDSSIVTQKKVNGVKVPADKTYSVELVSPILTYEEDIETLQE
                     IIRKLRKAGAFSESQNRTGIHIHLDGADHTPTSLRNFVNIIYSRNDLLYDSLQVEETR
                     KSYCKKMDKDLVERMNKKKPKTFNQTEDIWYEGYGPVRRQHYHNSRYHFLNLHSFFSG
                     VGTVELRGFNGTLHAGEIRAYIVLALAMNHQALTQKSASTKKPQTDNPKFAMRTWLNR
                     IGLIGDDYKNCREHLCKHLEGSAAWRFPRAA"
     gene            complement(1936..2592)
                     /locus_tag="PKH42_08970"
     CDS             complement(1936..2592)
                     /locus_tag="PKH42_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20605.1"
                     /translation="MNSTGLVKSISFNQTEIINWILYLHAPEQMIDCDPTYSKGLFYK
                     NTGIEEPAYKFDLEPQFDDVIKCDCRSLPVDDESFNCIMFDPPFLATKGKSLKADTGN
                     VINKRFTVYPSEKELHMMYTESLNEFHRVLKPGGILIFKCQDKVSSGKQYMSHCFIMN
                     EAVKIGFNPKDLFILLSKNRIVANWQMKNQKHARKYHCYFWVFEKITGKSLPTIYANH
                     "
     gene            complement(2676..3905)
                     /locus_tag="PKH42_08975"
     CDS             complement(2676..3905)
                     /locus_tag="PKH42_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA20606.1"
                     /translation="MKLERIPIDKLKAATYNPRKDLKPGDIEYEKLKKSIETFGYVEP
                     VLWNRRTGNIIGGHQRFKVLVELGQKEIDCVVVDMDLTDEKALNIALNKVSGEWDKDK
                     LMLLITDLQGSDFDVSLTGFDSIELDALFKDSLKEDIKEDDFDVDAELKKPALSKQGD
                     VWLLGPHRLTCGDSTKADTFTALMDGKLANLVVTDPPYNVNYEGTAGKIKNDNMNNEA
                     FYSFLLAAFKNTEAAMAKDASIYVFHADTEGLNFRKSFSEAGFYLSGTCIWKKQSLVL
                     GRSPYQWQHEPVLFGWKKSGKHNWYADRKQTTIWEFEKPKKNADHPTMKPVALVAYPI
                     LNSSLSNCIVLDPFGGSGSTLIACEQTDRICYMIELDEKYCDVIVNRYIDQIGTNEEV
                     FLIRDGKKIKHCDIEKP"
     gene            complement(3916..>4991)
                     /locus_tag="PKH42_08980"
     CDS             complement(3916..>4991)
                     /locus_tag="PKH42_08980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOA20607.1"
                     /translation="FDATTFDVSLTGFDDQEISELLGLKEEVVEDDFYEEAPTEPITK
                     PGDIWLLGEHRLLCGDCTDSAAVDRLMGGEKANCVITSPPYAMQRKNDYGGIPPDDYP
                     LWFLKVAQNIYRILDDSGSLFVNIKEHVENGQRSLYVFKTIIAMVESGWRYVDQLIWT
                     KTGLPGGWPNRLRNDFEPVHFFTKKEEIDWMVQFVEADEDKLKTIELDLIDMYEDIFH
                     FARSEKIKFKPKNVGKVSAQIRVSSKTNKSKGRSGNISVSGKFKKGIARAGNVLQITG
                     NQESLKHSAVFPVKLPAFFIKLTTDTKDIVYEPFAGSGTTLMAAEQLKRICYAMELSP
                     AYCDLIVKRWESFTGKTAKKLEV"
BASE COUNT         1340 a         1046 c          850 g         1755 t
ORIGIN      
        1 gtccttaatg caaactttgg attatcgtct tgtgtaacct tgtaagaagt acgtttctgt
       61 tccttagcga attcattgat gcgagctgca agtactgcaa aggatgatat cttctcgtaa
      121 gccaaattgt ctgaggggat gttataagta taaatgttat tttcaaagtc aaatgttata
      181 cccttacatc tttccgttcc aatttcctca acagcaacct tgaactcgtc caaagttgta
      241 gtagttttca agctcaaatc ttcagcaaat tttccatcca ttaatttttc ttcaatacca
      301 atggacttca taattaaatg ctgcttgctt gaaagcatat tcacaatatt cttaagtgta
      361 gtacctgagt gtccatccat gggaatgcta agttccaagt tatcaatgct tgatgtttcc
      421 tcaacttctt cttttgcacc ataatttgat ggctcctcaa aatctcggta aggactcact
      481 ctgccgccaa gagcggcttc atatggaatt ataagcccct cgggaacagg ttctgattct
      541 ggtatcggac tgtcatattc ttctgtaaca gccttaaaat cgtatagtcc ctgtaagtcc
      601 tcaaccagct cgtagttgtc gtttcccata agtactccgt tcttgtcgat gttgtagcca
      661 gccacctcgt aagcaaaagt aggtgcacct aaatattttg aagaagcatt gagttcttgg
      721 ctgattgcat ttactaatga ttttcttttt tcgcctgtaa tattgtagtt aatttgcatt
      781 ttttacatct cctttctttg taggtacata catcactcta aatgagaaaa atagcaagtt
      841 atattttaat gaaatattca aagagcggtt tcccgccctt tttaaggcag ccgccttagt
      901 taagcggctc ttggaaatct ccaagctgct gagccttcaa ggtgtttgca aaggtgctct
      961 ctacagtttt tgtagtcgtc accaataagc cctattctgt taagccaggt tctcattgcg
     1021 aattttgggt tgtcggtttg tggttttttt gtgcttgcac ttttttgtgt aagtgcctga
     1081 tggttcattg ccagtgcaag tactatgtat gcccttattt cccctgcgtg aagggttccg
     1141 ttaaaacccc ttaactctac cgttccgact ccgctgaaaa agctgtgaag gtttagaaaa
     1201 tggtatctgc tattgtggta atgctgcctt ctaactggtc cgtatccttc gtaccaaatg
     1261 tcctctgtct ggttaaatgt ttttggtttc ttcttgttca tcctttccac caaatctttg
     1321 tccatcttct tgcagtagct ttttctggtt tcttcaacct gaaggctgtc gtaaagcagg
     1381 tcgtttcttg agtagattat gttcacaaag tttcttaatg aagttggtgt gtggtctgct
     1441 ccgtcaaggt gtatgtgaat gccggttctg ttttgacttt ctgaaaaagc tcctgctttt
     1501 cttagcttcc taattatctc ttgcagcgtc tcaatgtctt cttcgtaggt tagaattggg
     1561 cttaccagtt ctacgctgta agttttgtct gcaggaacct tcacgccgtt tacctttttc
     1621 tgtgtaacta tgcttgaatc tgaaactatc ttccaaaccc taccgtctgt tgctgtaacc
     1681 tcgtgtgtgt catagcttcc ataacatctt tcaattgttc ctctcaagtg gtttgcaact
     1741 gttcttgccg cctttgttct tgtaatgcct gtcatttcta cttcgattcc aaatctgcct
     1801 tttaaaaatt ctgcacttgc cattttcttt tcccctttcc tttagtgtgt ttcttttgtt
     1861 atgtacatat atcactctaa aagggataaa tagcaaggta tatttttaaa aatacactta
     1921 ttttttattg aaatttcaat ggttggcgta tattgtcggc agtgatttac ctgttatttt
     1981 ctcaaaaacc cagaagtaac aatgatactt tcttgcatgc ttttgattct tcatctgcca
     2041 gttggcaaca attctgtttt tagaaagtaa gataaataag tcctttggat taaaccctat
     2101 ttttacagct tcattcataa tgaagcagtg gctcatatac tgcttgccgc tgcttacttt
     2161 atcttgacat ttgaatatga gtattccacc cggctttaaa actctgtgaa attcattaag
     2221 tgattccgtg tacatcatat gaagttcttt ttccgatgga tatacagtaa accttttgtt
     2281 tatcacattg cctgtatctg ccttaagaga ttttcccttt gttgcaagaa aaggagggtc
     2341 aaacattatg cagttaaatg attcatcatc cacaggaaga cttctacaat cacatttaat
     2401 tacatcatca aactgaggct ccaagtcaaa tttatatgca ggctcttcaa ttcccgtgtt
     2461 tttataaaat aaccctttag aataggtagg gtcgcaatct atcatttgtt ccggtgcatg
     2521 aaggtataat atccagttga tgatttctgt ttggttgaag gaaatgcttt taactaagcc
     2581 tgttgaattc attgcctttc ctcccttata tccgtcgaag gtttagtcga aagctttatt
     2641 aaaagcccac agaagcgaaa ctggcgcctc atattttacg gtttttcaat atcacagtgc
     2701 tttattttct taccatctcg tattagaaaa acttcttcat ttgttccaat ctgatcgata
     2761 tatctgttaa ctataacgtc gcagtatttt tcatccagtt caatcatgta gcagatcctg
     2821 tctgtctgct cacaagcaat tagtgtgctt cccgaaccac cgaaaggatc gagaacgata
     2881 cagttagata aacttgaatt taaaataggg taagccacca aggctacagg cttcatggtt
     2941 ggatggtctg cattcttttt cggcttttca aattcccaaa ttgtagtctg ttttctgtct
     3001 gcataccagt tatgcttgcc agactttttc caaccgaaaa gcactggttc atgctgccac
     3061 tgataaggtg aacgcccaag aacaagggac tgcttcttcc agatacaagt accggaaaga
     3121 taaaaacctg cttcggagaa tgattttctg aaattcagac cttctgtatc tgcatggaac
     3181 acataaatag aagcatcctt agccattgct gcttcagtat ttttaaatgc cgcaagcaga
     3241 aagctataga atgcttcgtt attcatatta tcatttttta tttttccagc agtaccctca
     3301 taatttacat tgtacggagg atccgtcaca acaagatttg caagtttacc atccataagg
     3361 gcagtaaatg tatcagcttt tgtggaatca ccgcaggtaa gcctgtgggg accaagcaac
     3421 catacatcac cttgttttga aagagcaggc tttttaagtt cagcatctac atcaaagtcg
     3481 tcttccttaa tgtcttcctt gagagaatct ttaaatagtg catctaattc aatagagtca
     3541 aaacctgtaa gagatacatc aaagtcagag ccttgtaagt ctgtaattaa aagcataagt
     3601 ttatctttat cccattcacc gctaacctta ttaagagcaa tatttaatgc tttctcatca
     3661 gttaaatcca tatccacaac cacacagtca atttcttttt gtccaagttc aactaaaact
     3721 ttaaatctct gatgaccgcc tataatattt cctgtccttc tattccacaa aaccggctca
     3781 acataaccaa aggtttcaat tgatttcttc agtttttcat actcaatatc tccgggcttt
     3841 aaatcttttc gtggattgta agttgctgct ttaagtttat ctataggaat tctttctaat
     3901 ttcattgtat ccattttaca cctctaattt tttggcagtc ttgcctgtga agctttccca
     3961 gcgtttaaca attaaatcgc agtaagcggg ggagagttcc attgcatagc aaattctttt
     4021 taactgttct gctgccataa gtgttgtacc tgaaccggca aagggttcat atacaatatc
     4081 ttttgtatct gttgttagct taataaagaa agcgggaagt ttaacaggaa atactgctga
     4141 gtgttttaat gattcctgat tacctgtaat ctgcaaaaca ttaccagccc ttgcaattcc
     4201 ttttttaaac ttaccgctta cactgatatt tccggacctg ccttttgatt tattagtttt
     4261 acttgaaact cgaatttgag cagatacttt tcctacattt ttaggtttaa acttaatttt
     4321 ctcagagcgg gcgaagtgaa atatatcttc atacatatct attaaatcga gttctatagt
     4381 tttaagttta tcttcatcag cttcaacaaa ctgtaccatc cagtcaattt cttctttttt
     4441 agtaaagaaa tgtacaggct caaaatcatt tcttaaacga ttaggccagc ctccgggaag
     4501 ccctgtctta gtccaaataa gctggtctac atatctccag cctgattcaa ccatagcaat
     4561 tatagtttta aaaacatata aggagcgctg gccattttca acatgctctt taatatttac
     4621 aaataaggaa ccactatcat caagtatcct ataaatgttt tgtgctactt ttagaaacca
     4681 caaagggtaa tcatcgggag gaatgccgcc atagtcattt tttctttgca tagcataggg
     4741 cggggaagtt ataacacagt tagctttttc tccacccatc aatctatcca cagccgctga
     4801 gtcagtacaa tcaccacaaa gtaagcggtg ttctcccaat agccaaatat ctcccggttt
     4861 agttattggt tcagttggtg cttcttcata aaaatcatct tctacaactt cttcttttaa
     4921 ccctaacagc tcgctgattt cctgatcatc aaaaccggta agagatacat caaaggtagt
     4981 tgcatcaaat t
//
LOCUS       DAOPUE010000131         4990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_16912_length_4990_cov_11.743060, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000131 DAOPUE010000000
VERSION     DAOPUE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16912_length_4990_cov_11.743060"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..263)
                     /gene="rpmC"
                     /locus_tag="PKH42_08985"
     CDS             complement(60..263)
                     /gene="rpmC"
                     /locus_tag="PKH42_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOA20608.1"
                     /translation="MKAKDLRKKSVEELNKSVVDLKTELFNLRFQLATGELDNPMRIN
                     KVKKDIARVKTVIRERELNINVE"
     gene            complement(253..696)
                     /gene="rplP"
                     /locus_tag="PKH42_08990"
     CDS             complement(253..696)
                     /gene="rplP"
                     /locus_tag="PKH42_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOA20609.1"
                     /translation="MLMPKRVKRRKQQRGRMTGIATRGNTITYGEYGLQAIEPAWITS
                     NQIEAARRAMSRAVKRGGQIWIKIFPDKPVTKKPAETRMGKGKGSPEYWVAVVKPGRV
                     LFEMSGVTEDVAREAMRLAMHKLPIKCKFVAKDTAAEKDGEADES"
     gene            complement(723..1460)
                     /gene="rpsC"
                     /locus_tag="PKH42_08995"
     CDS             complement(723..1460)
                     /gene="rpsC"
                     /locus_tag="PKH42_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOA20610.1"
                     /translation="MGQKVNPHGLRVGIINDWDSKWYADKKNFAENLTEDYKIREYIK
                     RENYQAGIAKIEIERFAGRVKVSVFTAKPGMIIGKGGTGVDILKNSIEKITGKTVIIN
                     VEEVKVPELNGQLVAENIASQLEKRISFRRAMKQAMQRTMKVGAKGIKTSVSGRLNGA
                     DMARTEGYSEGKIPLQTLRSDISYGFAEADTTFGKVGVKVWICRGEILRKPGSLLSDY
                     QEPTRPLNESKDNKKKRRSNYKRDKKQ"
     gene            complement(1482..1817)
                     /gene="rplV"
                     /locus_tag="PKH42_09000"
     CDS             complement(1482..1817)
                     /gene="rplV"
                     /locus_tag="PKH42_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOA20611.1"
                     /translation="MEAKAVAKYIRISPRKMKFVCDMVRGKQVDEALAILKFHPGKGS
                     RILEKVVKSAAANAENNFDMDRDKLYVVNAYANQGPTLKRWRPRAKGSAYKILKRSSH
                     VGAVVKERE"
     gene            complement(1835..2116)
                     /gene="rpsS"
                     /locus_tag="PKH42_09005"
     CDS             complement(1835..2116)
                     /gene="rpsS"
                     /locus_tag="PKH42_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOA20612.1"
                     /translation="MGRSLKKGPYCEPSLMKKIVEMNDANNKKVLKTWSRRSTIFPEM
                     IGHTLAIHDGRKHVPVYITEDMVGHKLGEFAPTRTYRGHDKSDKTSKVK"
     gene            complement(2143..2976)
                     /gene="rplB"
                     /locus_tag="PKH42_09010"
     CDS             complement(2143..2976)
                     /gene="rplB"
                     /locus_tag="PKH42_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOA20613.1"
                     /translation="MGIRKYKPTSPALRQMTVPTFEEITTDEPEKSLLTPLKSKAGRN
                     AHGRITVRHKGGGEKRKYRIIDFKRNKDGVPGKVATIEYDPNRSSYIALINYVDGEKR
                     YIIAPNKLQVGDTIVSGEDADIKIGNALPVRNIPVGTMIHNIELKPGKGGQMVRSAGN
                     AAQLMAKEGKYAQVRLPSGEVRMISMDCRATIGQVGNIDHENITIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPVTPWGQPTIGYKTRKKNKKSDKMIVSRRNK
                     K"
     gene            complement(3006..3296)
                     /gene="rplW"
                     /locus_tag="PKH42_09015"
     CDS             complement(3006..3296)
                     /gene="rplW"
                     /locus_tag="PKH42_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOA20614.1"
                     /translation="MRDPHDIIIKPIVTEASMNAMADKKYTFVVDKKSNKTEIKNAVE
                     SIFGVKVASVNTMNMLGKKKRMGVHVGKRPDWKKAIVTLTEDSKTIEFFEGI"
     gene            complement(3296..3919)
                     /gene="rplD"
                     /locus_tag="PKH42_09020"
     CDS             complement(3296..3919)
                     /gene="rplD"
                     /locus_tag="PKH42_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOA20615.1"
                     /translation="MPKVALYDITGSQIGDVELSDDIFGVKVNTHVMYEAVKNYLANQ
                     RQGTQSAKTRGEVRGGGRKPWRQKGTGRARQGSIRAPQWIGGGVVFAPKPRDYSYKLP
                     KKIKRLALKSALSSKVRDNEIIVVDSLVLDKPKTKDMVKILSNLKAGKKTLVVIPERD
                     ENVILASRNIPGVKTAYVNTINVYDILNCDSFLITKDAVNKVEEVYA"
     gene            complement(3946..4578)
                     /gene="rplC"
                     /locus_tag="PKH42_09025"
     CDS             complement(3946..4578)
                     /gene="rplC"
                     /locus_tag="PKH42_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOA20616.1"
                     /translation="MKAILGKKIGMTQVFNEQGEVIPVTVLESEGMVVVQKKTEEKDG
                     YNAIQVGFGDVKIKNVIKPKKGHFDKSKVEPKRFLREFRVDNIDDYEVGQKIGVDIFQ
                     AGEKVDVTGISKGKGFQGSIKRHGHARGDMSHGSKFHRLRGSLGASAGVGKVVKGTKA
                     HGHMGNEKVTVLNLEVVKVDADKNVILLKGAVPGPKKGLVKIREAVRKSK"
     gene            complement(4710..>4990)
                     /gene="rpsJ"
                     /locus_tag="PKH42_09030"
     CDS             complement(4710..>4990)
                     /gene="rpsJ"
                     /locus_tag="PKH42_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOA20617.1"
                     /translation="KAYDHQLIDQSAQKIVETAKSTGATVAGPIPLPTEKQIITILRA
                     VHKYKDSREQFEQRTHKRLIDITNPTPKTVDSLMKLNLPAGVDIEIKL"
BASE COUNT         1129 a         1196 c          717 g         1948 t
ORIGIN      
        1 actactttac cgattcttac ttttctgttg cctctttcca aagtaaatcc tccttccctt
       61 tactccacgt taatatttaa ttcgcgctcg cgaattacag tcttaactcg agcaatatct
      121 ttcttgactt tatttattct catagggtta tctaattccc ccgttgcaag ctgaaatctc
      181 aaattgaaaa gttctgtttt taaatccaca acagacttat tcaactcctc aactgacttc
      241 ttccttaaat ccttagcttt catctgcttc accatccttc tctgctgccg tatccttggc
      301 aacaaatttg catttgatag gtaatttatg catggcaagt ctcattgctt ctctggctac
      361 atcttctgta actcctgaca tttcaaataa tactctgccc ggtttcacta ctgctaccca
      421 gtattctggt gaacccttac ctttacccat acgtgtttcg gctggttttt ttgttaccgg
      481 tttatccgga aatattttaa tccaaatctg gccgcccctc tttactgctc ttgacatagc
      541 ccttctggct gcttcgattt ggtttgatgt tatccaagct ggctcaattg cctgcagacc
      601 atattcgccg tatgtgatag tattgcctct tgttgcaata ccggtcattc tgcctctttg
      661 ctgtttacgg cgttttactc ttttaggcat taacataatt atttcctcct tccttcaggg
      721 ctttattgct ttttgtctct tttataatta cttcttcttt tctttttgtt atccttgctt
      781 tcgtttagcg gtcttgtagg ctcttgataa tccgataaca atgagcccgg tttccttaag
      841 atttcacctc tgcatatcca aaccttaaca ccaaccttac caaacgttgt atctgcttct
      901 gcaaaaccgt agcttatgtc agaccttaaa gtttgaagag gaattttacc ttctgaatat
      961 ccttcggttc tagccatatc tgccccgttc aatcttccgg atacacttgt tttaattccc
     1021 tttgccccaa ctttcatagt tctttgcatt gcctgtttca ttgcccttct gaatgaaata
     1081 cgcttctcaa gctgacttgc aatgttctct gcaactaatt gcccgtttaa ctcaggaacc
     1141 ttaacttctt ccacattgat tattacagtc ttacctgtta ttttctctat gctgttcttt
     1201 aaaatatcaa caccggtacc gccttttccg ataatcatac ccggcttagc agtaaataca
     1261 cttactttaa ctctgccggc aaatctctca atttctattt ttgctatacc tgcttgatag
     1321 ttttctcttt ttatatattc acgaatttta tagtcttcag ttaaattttc tgcgaaattc
     1381 tttttgtcgg cataccattt tgaatcccaa tcattgatta taccaactct tagtccatga
     1441 gggtttacct tttgacccat cttttacctc ctttactgta gttattctct ttctttaacg
     1501 actgcaccaa cgtgactgct tctttttaat attttatatg ccgaaccctt tgctcttggt
     1561 ctccatcttt tgagagttgg tccttgattt gcatatgcat ttacaacata taatttatct
     1621 ctatccatat caaagttgtt ctctgcatta gctgccgcag atttcactac tttttctaaa
     1681 attcttgagc ccttgccggg atggaatttt aaaattgcca atgcttcgtc aacttgcttt
     1741 cctcttacca tatcacacac gaatttcatc tttcttggtg atattctgat atatttagca
     1801 actgccttag cttccatcag cgaatcctcc cttcttattt aactttcgaa gttttgtcgg
     1861 acttatcatg tcctctatat gttcttgtcg gagcaaattc acccagcttg tgtccgacca
     1921 tatcttcagt tatatataca ggaacgtgct ttctgccatc atgtatagct aatgtatggc
     1981 ctatcatttc agggaaaatt gttgatcttc ttgaccatgt cttcaatact ttcttattat
     2041 ttgcatcatt catttcaaca attttcttca taaggcttgg ttcacaataa ggtcctttct
     2101 ttaacgatct acccattcac tatttcctcc tttcaatcct gcttatttct tattccttct
     2161 gcttacaatc attttatcgg atttcttatt tttcttacga gttttataac cgattgtcgg
     2221 ttgtccccaa ggtgtaaccg gtgacggtct tcctattgga gctcttcctt caccaccgcc
     2281 gtgagggtgg tcgttaggat tcattacaga gcctctaacc gtaggtctga ttcccatatg
     2341 tctctttctt ccggccttgc ctattgttat attctcgtgg tcaatatttc ctacctgacc
     2401 gattgttgct ctgcaatcca tgcttatcat tcgaacttca ccgcttggaa gtcttacctg
     2461 tgcatatttt ccttccttag ccataagctg agctgcattt cctgccgagc gaaccatttg
     2521 tccgcctttt ccgggtttta attctatatt gtgaatcatt gtaccaaccg gaatatttct
     2581 tacagggaga gcattgccga ttttaatatc tgcatcctca cctgaaacga ttgtatctcc
     2641 aacctgtagt ttgtttggag ctataatata tcttttttct ccatcaacat aatttataag
     2701 tgcaatgtat gagcttctgt ttggatcata ctctattgtt gcaacttttc ccggaactcc
     2761 atctttattt cttttgaaat ctataattct atattttctc ttttcaccgc cgcctttgtg
     2821 acgtaccgta attcttccgt gtgcatttct tcctgctttg cttttcaacg gagttaaaag
     2881 agatttctca ggttcatcag ttgttatctc ttcaaatgtt ggaacagtca tttgtctcaa
     2941 cgccggagaa gttggtttat attttctaat acccatgaga ttttcctcct tctttaaatt
     3001 taagcttata tcccttcaaa gaattctatt gtcttgctgt cttctgttaa agtaacaatg
     3061 gcttttttcc agtcgggtct cttgcccaca tgtactccca ttctcttctt tttaccaagc
     3121 atgttcatcg tgttcacact tgctacttta acaccgaaaa ttgactccac tgcatttttg
     3181 atttcagtct tgttagactt cttatcaaca acaaaagtgt atttcttgtc tgccatagca
     3241 ttcatacttg cttctgttac tatcggtttt atgataatgt cgtgtggatc gcgcattatg
     3301 cgtacacctc ctccacttta ttaactgcat ccttagttat aaggaaagaa tcacagttta
     3361 atatatcgta aacattaatt gtatttacat atgccgtttt tacgcccgga atatttcttg
     3421 atgctaatat tacattttca tctctttcag gaataaccac caatgttttc ttgcctgctt
     3481 taagatttga taatattttt accatatcct ttgtttttgg cttatcaaga accaaactat
     3541 ccactacaat aatttcatta tcccgtactt ttgagcttaa tgctgattta agcgctaatc
     3601 tttttatctt ttttggaagc ttatagctgt aatctctcgg ttttggagca aatacaacac
     3661 ctccgcctat ccattgcgga gcccttatac ttccttgtct tgcacggcct gttccctttt
     3721 gcctccaagg ctttctgccg ccgcctctca cttctcctct tgtcttagca gactgagttc
     3781 cttgtctttg attagccaaa taatttttta ctgcttcata cattacgtga gtgtttactt
     3841 ttactccaaa tatgtcatca cttaactcta cgtcacctat ttggctgcct gttatatcat
     3901 aaagagctac ttttggcatc tttaatcctc ctttccacgc aattcttact tagatttcct
     3961 aactgcttct ctgattttca ctaatccttt tttaggtccc ggtacggcac cttttaaaag
     4021 tattacattt ttatcagcat ctacctttac cacttcaagg tttaatactg taactttttc
     4081 atttcccatg tggccgtgag ctttcgttcc cttaactacc tttcccacgc ctgccgaagc
     4141 tcccaatgaa cctctcaatc tgtggaattt ggagccgtgg ctcatgtcgc cccttgcatg
     4201 tccgtgtctc tttatcgatc cctggaatcc cttacctttt gaaattcctg taacgtcaac
     4261 tttttctcct gcttggaaaa tatcaacgcc aatcttttga ccaacttcat aatcgtcaat
     4321 gttatctact ctgaattctc ttaaaaatct tttaggttct acttttgatt tatcaaaatg
     4381 acctttctta ggtttaataa catttttaat tttaacgtct ccgaatccta cctgtatggc
     4441 attgtatccg tctttttctt ccgttttctt ttgaacgaca accatgcctt cagattccaa
     4501 aacggtaaca ggaataactt ctccttgttc attgaacact tgagtcattc cgattttctt
     4561 tcctaatatt gctttcattt tacacctcct aatttcttac agcggattgc ttgcgcaatc
     4621 attctaagca gagtgtgttg tctctacttt ttaaacatcg gtagaaattg ccatgcaatt
     4681 ccatcacacc gtttcaacga tgtatatcct tataatttga tttcaatgtc tacccctgca
     4741 ggcaaattca acttcataag cgaatcaaca gtctttggag taggatttgt tatgtcgatc
     4801 aatcttttgt gtgttctttg ctcaaactgc tctctagaat ctttatactt gtgaactgct
     4861 ctaagtattg ttattatttg tttttccgtg ggcagcggaa taggacctgc aactgttgct
     4921 ccggtacttt tagctgtttc cacgattttt tgcgcagatt gatcaattaa ttgatgatca
     4981 taagccttta
//
LOCUS       DAOPUE010000132         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_17121_length_4942_cov_10.410477, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000132 DAOPUE010000000
VERSION     DAOPUE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17121_length_4942_cov_10.410477"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4789)
                     /locus_tag="PKH42_09035"
     CDS             complement(<1..4789)
                     /locus_tag="PKH42_09035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOA20618.1"
                     /translation="MKQISFIDAEVVPESGKISDFGAIRPNGEYIHGASPDVIRGFLL
                     ETDFLCGHNIIAHDLKYLNKYLGQIKCAGVIDTLHLSALLFPQRPYHSLVKDDKLLTE
                     EINNPVSDCKKAMNLFYDETGAFNLLPDEMKRILYILLNDKKEFKDFFKYIDYKNEGT
                     NLEAEAGIRKFFYGKLCENSDIKNIIYTHPIELAYCLSLICADDEYSITPPWVLMTYP
                     QVDNVMNQLRGTKCIPGCEYCNETMDAVKGLNRFFGYDAYRSFDGVPLQEQAVKSAIN
                     GDSLLAVFPTGGGKSITFQVPALMAGRNTKSLSVIISPLQSLMKDQVDNLENFNITDA
                     VTINGLLDPIERAEAIKRVETGDAHILYISPESLRSKSIEKLLQDRKIARFIIDEAHC
                     FSAWGQDFRVDYMYIAEFIKKICEKKNLDYIIPVSCFTATAKQNVIDDICKYFKENLN
                     LELKLYTADSGRKNLTYKIIKNESSEKYETLRNLIEYNNCPTIIYVSRIKNAMELAKR
                     LTDDGYEARPYHGKMDKKEKSKNQEEFIKGEVDIMVATSAFGMGVDKKDVGMVVHYDI
                     PGSLEDYVQEAGRAGRDQSINARCYVLFNDGDLDKHFILLNQTKISIQEIQQIWIAIK
                     NTTKFRSNMSNSALEIAKKAGWDEGMYDLETRVYTAVAALENAGYIKRGQNMPRVYAD
                     SIMARNMKEVSEKIAGSDIFSETEKQQAARIMNKLISKRSRSRAGSEDAESRVDYISD
                     HLGIEKAQVIHVIQLLRQAGILSDAKDLTIFMDESSYGKKSFDKLYEYIELERFLLSQ
                     ISEITTTKNIKQLNELALDAGVKNSNPDKIKTIFNFWSIKGLVKRNAKGNNNHIKINL
                     LRDRDKALEILEARWAIAEFILKHIQEINVNNENTLEFSVLELMGEYNFNMQLFNKKA
                     APWQIEDSLFYLSKIGILKIEGGFFVIYNAISIERLIDDNRIRYKAEDYKTLKDFYKH
                     KIEMIHIVGEYAKKMMEDYNSALKFADDYFRLEYSSFLRKYFKGPKGDEIQRNMTPER
                     FRQLFGQLSPAQLGIINDKVSKYILVAAGPGSGKTRILVHKLASVLQLEDVKNEQLLM
                     ITFSRAAATEFKKRLGDLIGTKANYIEIKTFHSYCFDLLGLVGNIEKSNDIINNAIDA
                     ILNNEVEQSRITKTVLVIDEAQDMDANEYRLVKTLMERNEDMRVIAVGDDDQNIFSFR
                     GSDSKYMSYILNFPDSKMYELVGNYRSSKSIVDFSNYFVQNIKERLKSMPITAVTKEE
                     GNVAITKYKSSNLIVPVINKLLKDGILGSTCILTKTNEEALQIYALLDKNGIKAGMVQ
                     RGDNYDLKNLLEVRYFMEALKTSNEAPLINDEEWEYAKKKTFNRFIKSENLPLLKQII
                     SDFEEIASKYKYKSDFSMFIEESQEEDFYNDTDKVTVSTIHKSKGKEFDHVIMVLKDL
                     NLNNEEIKRQLYVGMTRAKKTLSIHYEGEFIQRKKGFMGMGLRNIKYEFDNEEYPDLE
                     IIAFQLKYSDVYLSNFYKTTRLVRSLNSGDMLRFDENGCLDKNGTQILLFSAKFKNEL
                     KTYVEKGYKPLSAKVNNILFWKQEDREKETLIVLPQLTFKKNPL"
BASE COUNT         1351 a          977 c          738 g         1876 t
ORIGIN      
        1 ataatggatt tttcttaaat gtcagctggg ggaggactat caatgtttct ttttctctgt
       61 cttcctgctt ccagaataat atattgttta ctttggcaga caatggtttg taaccttttt
      121 caacatatgt ttttaattca tttttaaact ttgctgaaaa aagcaaaatt tgggtgccat
      181 ttttgtccaa acatccattt tcgtcaaatc gcagcatatc accgctgttc agacttctaa
      241 caagtcttgt tgtcttgtaa aagtttgata gatacacatc gctgtatttt agttgaaagg
      301 ctataatttc taaatccggg tattcttcat tatcaaattc atatttaatg tttcttaagc
      361 ccatacccat gaatcctttt tttctttgga tgaattctcc ctcgtaatga atggacaaag
      421 tttttttagc ccttgtcatt cctacataaa gctgtctttt tatttcttca ttatttaaat
      481 ttaagtcttt taataccatt ataacatggt caaattcctt ccctttggat ttatgaattg
      541 ttgagactgt tactttgtct gtatcattat aaaaatcttc ttcctgagat tcttcaataa
      601 acattgaaaa atcggactta tatttgtatt tacttgcaat ttcttcgaaa tctgatatta
      661 tttgcttcaa aagcggcaaa ttctcgcttt ttataaacct attgaatgtt tttttcttcg
      721 cgtattccca ttcttcatca tttattaatg gagcttcatt tgatgttttt aatgcttcca
      781 tgaagtatct gacttcaagg aggtttttaa ggtcataatt gtctcctctt tgtaccattc
      841 ccgcttttat tccattttta tcaagcagtg catatatttg aagagcttct tcattggttt
      901 ttgttagaat acaggtagat ccaagtattc catccttaag cagtttattt atcacgggca
      961 caatgagatt gctgcttttg tactttgtta tggctacatt gccctcttcc tttgttactg
     1021 ctgtaattgg catacttttc agtcgttctt taatgttttg gacaaaataa ttgctgaaat
     1081 caacaatact ttttgaactt ctataatttc ccactaattc gtacatttta gaatcaggaa
     1141 aatttaaaat atagctcatg tattttgagt ctgagcctct gaatgaaaag atattctggt
     1201 catcatcacc tacagctatt accctcatat cttcatttct ttccataaga gttttcacaa
     1261 gtctatattc atttgcatcc atatcctggg cttcatctat aaccaaaaca gtctttgtaa
     1321 ttcttgactg ctctacttca ttgtttaaaa tggcatctat cgcattgttt attatatcat
     1381 ttgatttttc tatattccca actaaaccca gcaagtcaaa gcaataggaa tgaaatgttt
     1441 ttatttcaat gtaatttgcc tttgtcccta ttaggtcccc aagtcttttc ttaaactctg
     1501 ttgctgcagc ccttgaaaat gtaatcatta acagctgctc attttttaca tcttcaagtt
     1561 gcaaaacaga tgccagtttg tgtacaagaa ttctcgtctt tccgcttccc ggccctgctg
     1621 ccactaatat atattttgat accttatcat ttataattcc taattgagcc ggtgaaagct
     1681 gcccgaaaag ctgtctgaat ctttcaggcg tcatatttct ttggatttca tcacctttag
     1741 gtcctttaaa atattttcgc aaaaatgagc tgtattccaa tcggaaataa tcatctgcaa
     1801 atttaagcgc ggaattgtag tcttccatca tcttttttgc atattctccc actatgtgta
     1861 tcatttcaat cttatgtttg taaaagtctt ttagcgtttt ataatcttca gccttgtatc
     1921 tgattctatt gtcatctatg agtctttcta tgctgatggc gttgtatatt acaaaaaatc
     1981 ccccttcaat cttaagtatt cctatctttg aaaggtaaaa aagcgaatct tctatttgcc
     2041 agggtgcagc ttttttattg aatagctgca tgttgaaatt atactcaccc attaattcga
     2101 ggacagaaaa ttccaatgta ttttcattgt ttacattaat ctcttgaatg tgtttcaata
     2161 taaattctgc aatagcccat cttgcctcta atatctccaa tgccttatct ctgtccctta
     2221 aaagatttat tttaatatga ttattattcc ctttggcatt tcttttaaca agacctttta
     2281 tcgaccaaaa attaaaaata gttttaattt tatcggggtt ggaatttttt acacctgcat
     2341 ccagagcaag ttcatttaat tgcttgatat ttttagttgt agttatctct gatatctgcg
     2401 ataggagaaa gcgctccaat tcaatatatt cgtaaagctt atcaaaggat tttttaccat
     2461 agctgctttc atccataaat atagtcaaat cctttgcatc ggataatatc ccagcttgtc
     2521 tcaaaagctg aattacatgt ataacctgtg ccttttcaat tcccaaatga tcggaaatat
     2581 aatcaaccct ggattctgca tcttcgctgc ctgctctgct ccggcttctt ttggatataa
     2641 gtttgttcat tattctcgcg gcttgttgtt tttctgtctc actaaatata tcagagccgg
     2701 caattttttc agaaacttcc ttcatatttc ttgccataat actgtctgca tatacccttg
     2761 gcatattttg tcctcgttta atgtaacctg cgttttccaa tgctgcaact gctgtataaa
     2821 ctcttgtttc caagtcatac attccttcat cccacccagc tttctttgca atttcaagtg
     2881 ctgaatttga catgttagat ctaaacttag ttgtattctt tatggcaatc caaatttgct
     2941 gaatttcttg tatgcttatt tttgtttgat ttaaaagaat gaaatgtttg tctaaatctc
     3001 catcgttgaa aagaacatag cagcgagcat ttatgctttg gtctctgccc gcccttcctg
     3061 cttcctgtac ataatcttcc agtgagccgg gaatatcgta gtgtactacc atccccacat
     3121 cttttttatc tacacccatg ccaaaggctg aagttgcaac cattatatcc acttcgccct
     3181 taatgaattc ctcttgattt ttactctttt cttttttatc catcttccca tggtaaggtc
     3241 ttgcctcata accgtcgtca gtaagtcttt tggcaagctc catggcattt tttattctgg
     3301 aaacatatat aatggtagga caattgttgt attcgataag gtttcttaat gtttcgtatt
     3361 tttcagaact ttcattcttg attattttat aagtaaggtt ttttcttcct gaatctgctg
     3421 tatagagctt taattccaaa ttgagattct ctttgaaata cttgcaaatg tcgtctatta
     3481 cattttgttt tgctgtagct gtaaagcatg ataccggtat tatgtaatca agatttttct
     3541 tttcgcagat ctttttaata aattctgcaa tatacatata atcaaccctg aaatcctgcc
     3601 cccaggctga aaagcaatgc gcttcatcaa ttataaatct tgcaattttc cgatcctgca
     3661 acagcttttc tatggacttt gatctgagtg attccggtga aatatagaga atatgagcat
     3721 cgccggtctc aactctcttt atagcctcag ctctttctat gggatccaaa agtccgttaa
     3781 tagttacggc atctgttata ttgaaatttt ccaaattatc gacctgatct ttcattaaag
     3841 attgaagagg tgatattata acgctaagag attttgtatt tcttcctgcc ataagtgcag
     3901 gcacctggaa tgtgattgat ttgcctccgc ctgtggggaa aactgccaaa agagaatctc
     3961 cgtttattgc gcttttaact gcttgctcct gcaaaggaac tccgtcaaaa cttctatatg
     4021 catcataacc gaaaaaccta ttcagcccct ttactgcatc cattgtttca ttgcagtact
     4081 cgcagcctgg aatgcacttt gttccccgca gctgattcat tacattgtcc acctggggat
     4141 atgtcataag tacccaagga ggagtaatgg aatactcgtc gtctgcacat atgagagaca
     4201 ggcaataggc aagctctatt ggatgagtat aaataatatt tttaatatct gaattctcgc
     4261 ataacttgcc gtagaaaaac tttcttatac ctgcttctgc ttctaaattt gttccttcat
     4321 ttttgtaatc aatatattta aagaaatctt taaactcctt tttatcattc aacaatatat
     4381 aaagtatcct tttcatttca tcaggcagta aattaaaggc tcctgtttca tcatagaaaa
     4441 ggttcatggc tttcttacaa tcgctaacag gattgttaat ctcttcagtc agcagcttat
     4501 cgtcctttac caatgaatga tagggtcttt gaggaaataa aagagcagat aaatgcaagg
     4561 tgtctataac accagcacat ttaatctgcc ctaaatattt atttaagtat tttaaatcat
     4621 gtgcaattat attatgtcca cataaaaagt ctgtttctaa aaggaatccc ctgatgacat
     4681 caggtgaagc accatgaata tattctccgt ttggtcttat agcaccaaag tcgctaatct
     4741 taccactttc cggcactact tctgcatcaa taaatgatat ctgcttcata atacccgcca
     4801 tcttaagttg aataatatgt atttagctca ttatatagca tattgaaaat aatttcaata
     4861 ttgatatgac aaggagatgg ggattacttt aggcgttctt ctatcagttt tgccagctgg
     4921 gcggcatgga agtttatttc tt
//
LOCUS       DAOPUE010000133         4814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_17720_length_4814_cov_10.773272, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000133 DAOPUE010000000
VERSION     DAOPUE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17720_length_4814_cov_10.773272"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1055)
                     /locus_tag="PKH42_09040"
     CDS             complement(240..1055)
                     /locus_tag="PKH42_09040"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOA20619.1"
                     /translation="MNFMSGFLKGLALGIAAIAPGISGGALAVIFGLYDKITYFIAHF
                     TRNFKENVLYFMPVGIGGVVGVLIFSRIIEYLFHFHEVEVKYAFIGLMFGTIPLVVKE
                     ANKKGYKQRYLIPFIITLSITLVAAYFEKDEINIIMNSGTAPLSLIIYGIIIGFGTII
                     PGISASILLMYMGAYEIVISAISNIQISIIFFLAIGFILSILLFSKIICMLFEKAYGI
                     TYYAILGLVLGSVLSIFPGFYLNFDYLLNLIILLACCLLSYSLSKLGKGKTIS"
     gene            complement(1150..1980)
                     /gene="amrS"
                     /locus_tag="PKH42_09045"
     CDS             complement(1150..1980)
                     /gene="amrS"
                     /locus_tag="PKH42_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOA20620.1"
                     /translation="MSKLICTICPHHCNLNEGQTGLCRARKNINGEITAINYGQLTAL
                     ALDPIEKKPLNRFYPGSNILSVGSFGCNLKCLFCQNYHISMASKNEVKTQFVKPEELT
                     NIALDLKDRKNIGIAYTYNEPLVGYEYVLDCAKLARDKNLKNVIVTNGCFCEEPMKKL
                     LPFIDALNIDLKGFTYEFYKKIGGDLDTVKNFIKLAAKHCHVEITTLIIPGENDTEDE
                     IRELSEFIFSVSPEIPLHVSRFFPCYKMLDKRPTDVKKVYDLANIAREKLKYVYEGNC
                     "
     gene            complement(1977..3362)
                     /gene="amrA"
                     /locus_tag="PKH42_09050"
     CDS             complement(1977..3362)
                     /gene="amrA"
                     /locus_tag="PKH42_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOA20621.1"
                     /translation="MMIVNSFIVPHPPLIIPEIGRGEEIKIQKTIDAYHNIAKQISEI
                     KPDTIIITTPHSIMYSDYIHISPGKRAKGSFREFGQRDVTMEVEYDVELVGEISNEAE
                     KAGISAGTLGEKNSSLDHGVMVPLYFVNKYYNNYKLVRISISGLSYLEHYKFGKCIKK
                     AAEKSNKKIVFIASGDLSHMLKETGPYGYREEGVIFDREVTKAMDSGNFSEFLKFDED
                     FCEAAAECGLRSFIIMAGALDGKKLSSKLLSYQGPFGVGYAVAEFKVLGDDEGRRFDE
                     IYLKTEMERIESLKSEEDEYVRLARQTLESYVINRKVIKRPDNLSPELIKNKAGVFVS
                     LKLDGSLRGCIGTISPTTSSIADEIIRNAISAGTEDPRFPPVTEDELSRLEYSVDVLG
                     KAEKIESMDELDPVRYGVIVSKGHRRGLLLPNLEGVETAEKQISIALRKAGISPNEDY
                     QLERFEVVRHK"
     gene            complement(3424..4674)
                     /locus_tag="PKH42_09055"
     CDS             complement(3424..4674)
                     /locus_tag="PKH42_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOA20622.1"
                     /translation="MDKSKGKLTKLERSWILYDVGNSAFILLISTIIPIYFKNIASSD
                     GVLPADSTAYWGYAMSTSTLIVAILGPVLGALADTKGYKKPLFTFFLMSGVLGCAALS
                     LPVPWIIFLALFVFARVGISGSLIFYDAMLPDVTSDERMDLLSSHGYAWGYIGSCIPF
                     TISLVLILFADKIGIATALATGIAFILNALWWFLVSLPLLKNYKQLHYVEVDNAPMAM
                     AFKRLGRTFGDIKKHKEIFIFLLAFFFYIDGVDTIIGMATSYGKDVGISDNNLLLALL
                     LTQVVAFPFAIIFGKLSKRYKTSELISVCIIGYFLITLFALQLDKAWEFWFLAVCVAV
                     FQGAIQALSRSYYAKIIPKESSSEYFGFYDIFGKGASFTGTTLMGITTQITGNSKFGV
                     LTICIMFIIGYVIFRKAVKMQTVS"
BASE COUNT         1520 a          983 c          727 g         1584 t
ORIGIN      
        1 ttatgtaaaa ataaaaaata aaaaaataaa gaaataaagt aaaagaataa agaataaatt
       61 tgtgttgagt acttcctttt accggaatac ccttgtattt cacctcccta tttttcatat
      121 tttattgtat cactaagctg ttatccagtc aaggtaagtt aaaaaatatt gtttattttt
      181 caattaaatg aaaagatttt gtaacaattt ccaactctcc tagtaaaagt ataaacaact
      241 taagaaattg tttttccttt tcctaatttt gacaatgaat atgacagtaa acagcatgct
      301 aatagtatta ttaagtttaa taaataatca aaattcaaat aaaaaccagg gaatatcgaa
      361 agaacggaac ctaatacaag gcctagaatt gcatagtatg taattccata tgccttttca
      421 aaaagcatgc atattatttt tgaaaataac aatatactca gtataaagcc tatggcgaga
      481 aaaaaaatta tggatatttg aatatttgaa attgcactta tgactatttc ataagcaccc
      541 atatacataa gcagtataga agcgctgata ccgggtataa ttgttccaaa tccaattatg
      601 atgccataaa taatcaggct cagaggagcg gttcctgagt tcattattat attaatttca
      661 tctttttcaa aatatgcagc aactaatgta atgctcaggg ttattataaa aggtattaaa
      721 tatctttgtt tatagccttt tttatttgct tcttttacta ccagaggtat ggtaccaaac
      781 atcaagccta taaaagcata ttttacttcg acttcgtgaa aatggaaaag gtattcaata
      841 attctgctga aaattaatac tccaacgact cccccaatac cgacaggcat gaaatacaat
      901 acattttcct taaaattcct ggtaaaatga gcaataaagt atgtaatttt gtcatacaaa
      961 ccaaatatta cagccaaggc tcctccgctg attccgggag ctatagccgc aatgcccaat
     1021 gccaaaccct ttaaaaatcc cgacataaaa ttcatcacac acacctgcca gtatactctt
     1081 gtttaattat atgtccatat ggtaatttta gcaataaaaa atcgtacgca gtctcattca
     1141 ccaaatcttt cagcagtttc cttcatagac gtatttgagt ttttctcttg ctatatttgc
     1201 caaatcataa acctttttta cgtctgtcgg ccttttatca agcatcttgt agcaagggaa
     1261 aaacctagaa acatgaagag gtatttcagg cgacactgaa aatataaatt ccgagagctc
     1321 ccttatttca tcctccgtgt cattttcacc aggtattata agagttgtta tttccacatg
     1381 acaatgtttt gctgcaagct taataaaatt tttaactgtg tccaagtccc cgccgatttt
     1441 tttataaaac tcataagtaa acccttttaa atcaatattc agagcatcaa taaaaggaag
     1501 tagttttttc atcggctctt cacaaaaaca accgtttgta acaatcacat tctttaaatt
     1561 cttatctctt gccaattttg cgcagtctaa aacatattca tatcccacca aaggctcgtt
     1621 gtaggtatat gctatgccta tattttttct gtcctttaaa tccaatgcta tatttgttaa
     1681 ttcttcaggt tttacaaatt gagttttaac ctcatttttg gatgccatgg atatatgata
     1741 attctggcaa aataaacatt ttaagttgca tccgaagctt cctacagaca gtatattgct
     1801 gccgggataa aacctgttca aaggcttttt ttcgattggg tcaagggcaa gagctgttaa
     1861 ttgaccgtaa ttgattgccg taatttcacc gttaatattt ttccttgccc tgcataaacc
     1921 agtttgacct tcatttaaat tgcagtgatg cgggcatata gtgcaaatta atttgctcat
     1981 ttgtgcctta ccacctcaaa tctttccaac tgatagtctt catttggaga aatacctgct
     2041 tttctcaaag ctatggagat ttgtttttca gctgtttcaa ccccctccaa attgggtaat
     2101 aaaagccctc ttctatgtcc ttttgaaaca atgacaccgt atcttaccgg atcaagttca
     2161 tccattgact ctattttttc tgctttaccg agaacatcta cgctgtattc cagtcttgag
     2221 agttcatctt cagtgacagg cggaaacctc gggtcttctg taccggcact tattgcattc
     2281 cttattattt catcagctat gcttgatgtt gtcggagaaa ttgttccaat gcagcctctt
     2341 aagctgccgt ctagtttcaa ggatacaaat actcctgcct tgtttttaat aagttcaggg
     2401 ctcaaattgt ccggtctctt aattaccttt cggtttatta catagctttc caatgtttgg
     2461 cgggcaaggc gcacatattc atcctcttct gatttcaatg actcaatcct ttccatttcg
     2521 gtttttaagt atatttcatc aaatcgacgc ccttcatcat ctcctaaaac cttaaattct
     2581 gcaactgcat agcctactcc aaaaggtcct tgataagaca gaagctttga gcttaatttt
     2641 ttcccgtcca atgcgcctgc cattattatg aaggagcgaa gtccgcattc agcagcagct
     2701 tcacagaaat cttcgtcaaa tttaagaaac tccgagaaat ttccgctatc cattgccttg
     2761 gttacttccc tgtcaaatat cactccctct tccctgtaac cgtaagggcc tgtttcctta
     2821 agcatgtgag acaaatcccc gcttgctata aaaacaattt ttttgttgct tttttctgct
     2881 gcctttttaa tgcattttcc aaatttgtaa tgctctaaat aagaaagtcc cgatatggaa
     2941 atgcgcacca acttataatt attgtagtat ttattcacga aataaagagg caccataaca
     3001 ccgtggtcaa gcgagctgtt tttctcaccc aaagttccgg cagaaatccc agctttctct
     3061 gcttcatttg aaatttcacc gactaactca acatcatatt ctacttccat tgtaacatca
     3121 cgttggccaa attcccggaa gctgcccttt gccctcttcc ccggtgaaat atgtatataa
     3181 tctgaataca ttattgaatg gggagttgtt ataattattg tgtcaggttt aatttcagaa
     3241 atttgttttg caatattgtg atatgcatct attgtctttt gaattttaat ttcttctcct
     3301 cttcctatct caggaattat caatggagga tgcggaacta taaaactatt cacaatcatc
     3361 ataatttctc ctttctttct tatatgattg ctatgaataa catatgtcat tccaaaagct
     3421 atctcatgag acagtctgca tttttactgc ttttctgaat attacatatc ctattatgaa
     3481 cataatacaa atggtcaata caccgaattt tgagttgccc gtaatttgtg tcgtaattcc
     3541 cataagcgtt gttccggtaa aggatgcacc tttaccaaaa atatcgtaaa agccgaaata
     3601 ttcgcttgaa ctctcctttg gtattatttt tgcgtaatat gagcgggaca aagcttgaat
     3661 cgctccctgg aaaaccgcta cgcatactgc cagaaaccaa aattcccaag ctttatcaag
     3721 ctgcagcgca aacagtgtaa ttaaaaaata acctatgatg cacacgctga ttagctctga
     3781 agtcttatac cttttagaca gcttgccaaa tattattgca aaaggaaaag caaccacctg
     3841 tgttaaaaga agcgccaata atagattatt gtcacttatt ccaacgtcct tgccgtatga
     3901 tgtggccatt cctataattg tatctacccc gtctatataa aagaaaaagg caagcaaaaa
     3961 tatgaatatt tccttatgtt tttttatatc gccaaaagtt cttccaagcc gcttaaatgc
     4021 catagccatg ggagcattat ccacctccac gtaatgaagc tgcttgtaat ttttaagaag
     4081 aggcaggctt actaaaaacc accataatgc atttaatata aaagctattc ccgttgcaag
     4141 ggcggtggca attccaattt tatctgcaaa taaaatcaac acgagactga tggtaaaagg
     4201 aatacagctt cctatataac cccaggcata accgtgggaa gacaataaat ccatcctttc
     4261 atcacttgtt acatccggaa gcatggcatc gtaaaatatc agacttcctg aaatacctac
     4321 ccttgcaaat acaaacaagg ctaaaaatat tatccacgga acgggaagtg atagtgctgc
     4381 gcacccaagt accccggaca ttaaaaaaaa ggtaaacaaa ggtttcttat atccttttgt
     4441 atctgctaat gctcctaata caggtcctaa tatcgcaact atcaaagttg aagtcgacat
     4501 ggcatatccc caataagcag tcgaatcggc aggtaaaact ccgtccgatg aagcaatatt
     4561 tttgaaatag attggtatga ttgttgatat gagcagaata aatgcagaat tcccgacatc
     4621 atacaaaatc caggaccttt ccaatttagt taattttcct ttagatttat ccataatctc
     4681 caccttaatt gtatattttt ctttagttac attttcggtg tgtcaccata ccttttaatt
     4741 atattattat atattgaaag attttacaag caaaagaaaa actgccaata taagcagttt
     4801 atccgaaatt tctg
//
LOCUS       DAOPUE010000134         4803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_17792_length_4803_cov_8.489048, whole genome shotgun sequence.
ACCESSION   DAOPUE010000134 DAOPUE010000000
VERSION     DAOPUE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17792_length_4803_cov_8.489048"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PKH42_09060"
     CDS             complement(<1..892)
                     /locus_tag="PKH42_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA20623.1"
                     /translation="MESAKKYKYMGFVLLQSVIYGFGNPLTKIAFESITPFWSLAVRF
                     TTAFLVFMLLFGKRIIASLKSVEPKVYLPASICMAAAYITCNLALNWTSATNVGFLMS
                     LPVIFAPALALLLYKVKYRLIHIPIQIVVVIGLYLLCFDGEKLVFGKGEVTALITALS
                     IAGALVFGEESLKQLDAVAVSAAQAGITALISLPCALIFESIEVLSRVTLPAWLVVLY
                     LAIFCTNIAYMLQNTALKHISAAAVSMLQCTQPILTAIISYFLLREKLSTLGVIGAVI
                     IIICIIAENIITAKEQAPSEN"
     gene            complement(1188..2696)
                     /locus_tag="PKH42_09065"
     CDS             complement(1188..2696)
                     /locus_tag="PKH42_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009572688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOA20624.1"
                     /translation="MDIMQYLLIPITEPMLSVLVAIGVFVGIYVGAIPGLSGTMAVSL
                     LVSFTFGWETNSAISLMMGIFSGAVYGGSRSAILLNIPGAPAAVATSFDGYPLAQKGL
                     AGETIGVATISSVLGGFLGCIALAFLAPLVSSIALKFAPRDFLLLAFMGMMLVSSLGS
                     KSLARALVTAAIGVLLGTVGMDPISAYPRFTFGSTNLLTGVDYITAMIGLFGVSEALM
                     QIRDLDIKAVKQKIDRIVPSFLSIKKNFPLTIRSSILGIIVGALPGAGGDIAALLAYD
                     QAKRTVRHPETPFGKGAVEGVIAPETANNAAIGGAFIPMLTLGIPGDAITAVMIGALL
                     IHGLQPGPMLMQETPHLFYLIVSTLAISNVFLLIFGLAGIKIFTKLVEIPKGRLLPII
                     IILSVISTYAVNNSLTDILWMVGFGILGYFFKVYDYPVAPTVLGIILADLIENNYRRA
                     VISAGSILKLVGGIFTSPLSMILLGIIIFSFVTQSDKYNKWRDKRDAKKAAK"
     gene            complement(2708..3196)
                     /locus_tag="PKH42_09070"
     CDS             complement(2708..3196)
                     /locus_tag="PKH42_09070"
                     /inference="COORDINATES: protein motif:HMM:NF018979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOA20625.1"
                     /translation="MTGKKKIINKPGEKGFSIIMLVIGILFLIESIRMFIEEPKSSSY
                     GALPLFLSCSIVILMLKILLVEYRNNGTNKEKVSLKDSIISVKNYAFTQDIVFEFIFM
                     AAYCILLILGLGFEISSSLFLLISMTYLMRGQLLKNSIYTLITMAFILIVFKTVFQVI
                     LP"
     gene            complement(3271..4293)
                     /locus_tag="PKH42_09075"
     CDS             complement(3271..4293)
                     /locus_tag="PKH42_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOA20626.1"
                     /translation="MNNLRKLISQILMLSLIVTMLVSCSNVAQETETPFPENDITGVV
                     QWGAGGGTDSLMRPLASITEDILDVSIVVQNKPGATGAIGAHYVYDLPANGYNLLMGA
                     ENPQLYTMLGISELTYENFEPIYLIGDEKVGIIVGKDSKYESFTELIEDALANPGEVS
                     ISSTGTGGMPWSVSSFINAVTGAEFKQIPFDSDATALSAVLGGHADFTAAKVQTALQS
                     YKAGEIKFLSLMSLDKVEVLPEVPLVIEDYPDFEQYLPWGPFYGVFVKEGTPKETINI
                     LSKAFSEAGEDETYQKLLKDFNVTSLALTGDEAKEYLSNWQRNTAEALYRSGAIDKSP
                     EELGIE"
BASE COUNT         1625 a          918 c          722 g         1538 t
ORIGIN      
        1 gattttcgga aggggcttgt tctttagcag ttattatatt ttctgcaatt atacatatga
       61 ttattattac tgcaccaata actcctaaag tgctgagctt ttctctaaga agaaaatatg
      121 aaattattgc tgtcagaatg ggctgggtac attgaagcat tgaaacagct gctgctgata
      181 tgtgttttag ggctgtattt tgaagcatat aggcaatatt ggtacaaaat attgcaagat
      241 acaaaaccac taaccatgca ggcagggtta cacggcttaa aacctctatg ctttcaaaaa
      301 tcaatgcaca gggcaagcta attaaagcag ttatgccggc ctgagcagca gatactgcca
      361 cagcatccag ctgctttagg gattcctcac cgaataccag cgctcctgct attgacaggg
      421 ctgtaattaa tgcggttacc tctccttttc cgaaaaccag tttttctccg tcgaagcaga
      481 gaagatataa gccgataacc acaacaattt gtataggaat gtgtatgagc ctgtatttaa
      541 ccttatataa caagagtgca agtgcaggtg caaaaatcac aggcaggctc attaaaaatc
      601 ccacatttgt tgcagaagtc cagttcaatg caagattgca ggtaatgtaa gccgctgcca
      661 tgcatatgct tgcaggcaga taaaccttag gctcaacgct ttttaaggat gcaataatcc
      721 ttttgccgaa aagtaacata aaaaccaaaa aggctgttgt gaaacggacg gcaaggctcc
      781 aaaaaggagt aatactttca aaggcaatct tggtcagcgg attgccaaag ccgtaaatga
      841 cgctttgaag taaaacaaaa cccatatatt tatatttctt agcactttcc atggcaaacc
      901 tgctttcatg attgatatga ctgcattacg ggtacagacc ctggaactca gggaacgcga
      961 tggaatctcc tgcaattgtt atcgaaattc cgtggatgta atttagatga caggtcttga
     1021 attcctatta taaaatctcc tgcaaacgtt attaatatta tataataaaa actcaaatga
     1081 ttcaattagt atttacgtta aaacaaaggt attgtgagac aaataatagt tggtctccct
     1141 aatattcttc aattaacagt aaataaacaa ttgcattata aatgctatta tttagcagcc
     1201 ttctttgcat ctcttttatc tcgccattta ttatatttat cagattgtgt tacgaatgaa
     1261 aatataatta ttcctagcaa aatcattgaa agagggctgg taaatatacc accaactaat
     1321 ttcaaaatgg aacctgctga tataacagct cttctatagt tgttttcgat taaatctgct
     1381 agtattatac ctaatactgt aggtgcaaca ggataatcat atactttaaa gaaataccct
     1441 aaaataccaa aaccaaccat ccataaaata tcagttaaac tattattaac tgcatatgtg
     1501 ctaattacag ataatattat tataattggt aacaacctgc cttttggaat ttcaactaac
     1561 ttagtaaata tcttaatccc ggcaagaccg aatattaata aaaatacgtt tgagatagct
     1621 aaagtactaa ctattaaata aaacaaatgt ggtgtttctt gcattaacat cggcccaggc
     1681 tgtaaaccgt gaatcaataa tgcacctatc ataacagcag ttatggcgtc tcctggaata
     1741 cccaaagtta acataggtat aaaggcaccg ccaatagcag cgttattagc tgtttcagga
     1801 gctattactc cctctacagc accttttcca aaaggagtct ctggatgtcg aacagtcctt
     1861 tttgcttgat catatgctaa taatgctgct atatctccac ccgccccagg taaagcacct
     1921 acgattatgc ctagtatcga tgaacgtata gttaaaggaa aattcttctt tattgataaa
     1981 aaagaaggta cgattctatc aatcttttgt tttactgctt taatgtctaa atctctgatt
     2041 tgcattagtg cctcagaaac tccaaataaa ccaatcattg cagttatata atctacacct
     2101 gtcagcaaat ttgtactacc aaaggtaaat ctaggatatg cacttattgg gtccatgcct
     2161 acagttccta ataaaacacc tatagcagct gtaaccagag ctcttgctaa tgacttggag
     2221 cctaagctgc ttactaacat catacccata aaagctaata gtaagaaatc tctaggtgca
     2281 aatttcaaag ctatgcttga aacaagaggc gctaaaaaag ctaaagctat acaaccaaga
     2341 aatccaccca atacagatga aattgttgca acaccaatag tttctcctgc cagtcctttt
     2401 tgagccaatg gatacccatc aaaactagta gctacagcag caggagcgcc tggaatattt
     2461 agcaatattg cggatcttga gcctccatat acggctccgg aaaaaatccc catcattaat
     2521 gagattgcgg aattagtctc ccaaccaaat gtaaacgata caagaagtga tactgccatt
     2581 gttccagata aacccggtat agccccaaca tatattccaa caaatacgcc aatagctacc
     2641 agaactgata gcattggttc agtaattggt attaataagt attgcataat atccattatg
     2701 tcactcctta aggtaaaata acttgaaaca cagtcttgaa tacaattaat atgaaagcca
     2761 ttgttatcaa tgtatatatt gagttcttca acaattgtcc cctcattaaa tatgtcattg
     2821 aaataagtaa aaataatgat gatgatatct caaatccaag acctaaaatt aacaatatac
     2881 aatatgctgc cataaaaata aattcgaata caatatcttg agtaaatgca taatttttta
     2941 cagatattat cgaatcctta aggctgactt tctctttatt tgtgccatta tttctgtatt
     3001 caactaataa aatctttaac attaaaataa ctatagaaca actcaagaac agtggtaaag
     3061 ctccataaga agacgacttt ggttcttcta taaacattct tattgattca attaaaaata
     3121 atatcccaat aactaacatt attatagaaa aacctttttc tccgggttta tttataatct
     3181 ttttttttcc tgtcatatta tacacctatt tttcctatat ttatataaga gctttagcta
     3241 tattgctata gtttcttata ttattaaaaa ttactcaatt cctaattcct caggtgattt
     3301 atcaatagca ccactcctat ataaagcttc cgcagtattc ctttgccaat ttgataaata
     3361 ttcttttgct tcatcacctg ttaaggccaa agatgtcaca ttaaaatctt ttagcaactt
     3421 ttgataagtc tcatcttcac cagcttcact gaaagctttg cttaatatat taattgtttc
     3481 ttttggagtc ccttctttta caaatacccc ataaaatggt ccccatggaa ggtattgttc
     3541 gaaatctggg taatcctcta ttactagggg tacttccgga agcacttcaa ctttatcaag
     3601 tgacataagg cttaaaaatt ttatttctcc agccttatag gattgcaaag ctgtttgaac
     3661 ttttgctgcc gtaaaatctg catgaccgcc taaaactgct gacaatgctg ttgcatcact
     3721 gtcaaaaggt atttgtttaa attcagcacc agtgactgca ttgataaatg aacttactga
     3781 ccaaggcatg cctccagttc cagtactgga aatacttact tcacctgggt ttgccaatgc
     3841 atcttctatc agctcggtaa atgattcgta ctttgaatct ttacctacaa taattccaac
     3901 tttttcatca ccaattaaat atattggttc aaaattttca tatgttaact cagatattcc
     3961 aagcattgtg tacagttgtg gattctcagc acccattaat aaattataac cattggcagg
     4021 taaatcataa acataatgag cacctatcgc ccctgttgca ccgggtttgt tttgaactac
     4081 aatacttaca tctaagatat cttctgttat tgatgctaat ggtctcatta aagagtcagt
     4141 accaccacct gcaccccatt gaacaactcc tgtaatgtca ttttcaggga atggagtttc
     4201 tgtttcttgt gcaacattac tgcaactaac taacatcgta acaattaaac ttaacattaa
     4261 tatttgtgat attaattttc tcaaattatt catagcatcc tccttaaatt attttccgaa
     4321 ttgtaaactt aaatgagtca atatcttttt aattccattt ttcaaaaaat ttctgtgcgg
     4381 catcgtcttt tgccaatcgc tctgataatc agtaattttg aacactttca tgattcctca
     4441 tttaattttt gttattgtat taatatatta tacgattaga gcatcggaag acacatttaa
     4501 ttttaataaa taaggataac ataattgcaa ctaccaagta taacgaaaac tttaatatta
     4561 taataattat aatatctaca acagcagact tcaataatta tcataaataa aataaaattt
     4621 tgttaatttt gttaacaagg ttaattgaaa aagtaaatgc aacgtagagg tttcatctac
     4681 tttttctttt tataattttg ttaacaaata ggcaagttgt aaaatgaatt gcccaaaaaa
     4741 ataaaaaaag atccattcct ggatcaaatc agttatactt atgttgaaga caaaagtgac
     4801 tgg
//
LOCUS       DAOPUE010000135         4753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_18058_length_4753_cov_10.165815, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000135 DAOPUE010000000
VERSION     DAOPUE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18058_length_4753_cov_10.165815"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1378
                     /locus_tag="PKH42_09080"
     CDS             95..1378
                     /locus_tag="PKH42_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA20627.1"
                     /translation="MIIHVVQPGETIYSIANQYNISPQKIIIDNELENPNVLVPGQTL
                     VILIPDVIHVVQPGETLADIARQYNTTTVNLLQLNPFIEYADQIVPGDQITISNTADK
                     RGLIHVLGYAYPHIDRTVLLKTLPYLTYLTIFTYGITEEGELIDIEDEDIIRMARDFG
                     VAPLMLISTLTEEGTFSNELAHVILNDPVAQNNLIENVLQNLREKGYYGLDIDFEYVL
                     PEDEEAFINFIQNVHDRISPEGFVLFTALAPKTSADQPGLLYEAHNYPRIGEASDRVL
                     LMTYEWGYTYGPPMAVSPVDRVREVLDYAVTEIPREKIYMGIPNYGYDFILPYVQGVS
                     RADSVSNVEAVDLAADVGAYIQYDEVSQAPYFIYYDEQGRQHRVWFEDARSILAKLDL
                     FSEYGFEGVGYWNVMRYFPQNWLLVSSLYDIAKLV"
     gene            1454..2641
                     /locus_tag="PKH42_09085"
     CDS             1454..2641
                     /locus_tag="PKH42_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20628.1"
                     /translation="MNNKNYRVLKFFEPVNKKDKSRGYVRLDMRGNRGNMIVAAENLG
                     DAKSSSEVYLYKDKHNKIKLGDINNKKGIIKKKLTFENSNAIEDYNVCAVVKDGKIVL
                     YSSLFSVSSQDIVRKLEEDDNEQEKDIFADKKQKFQESKPIHTKEKEQINIKDTRGAI
                     EPKEVIETKEIIEPKGLREVREIREKGMAKAAENKPEDETKEEVKKVVQEPVKTIKVK
                     ETIKEDSEETMETPEKSSKKDDISNQGYRNRFDEKLYKTLREFKEIEPLSVKLKNFNW
                     WYIPYDEMGIKTGVLPYYNQIISAYYPYPMSNRVTTCTSLMKKYGHYIFGIYKENDEI
                     ARFIYGVPGEFTKEEQPYKGVTGFKNWSYANKDNKGESGYWLAFVNPKTGEITDPPQV
                     SLT"
     gene            2807..3385
                     /locus_tag="PKH42_09090"
     CDS             2807..3385
                     /locus_tag="PKH42_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20629.1"
                     /translation="MPPISTVMHFGKKFMESYDKVDIPNFIIGIILPSTLNDDQLEEL
                     HNVDEDGNIDVREFYEKFDFKTMSLKDSSIVLGYYCHLWLDEYYKFNASKLKIHNKQN
                     LKGEELNSAVKNLLNYYDKKAIGSFYEKYTEDIKAVQSYIFAASNIDNSKKKLLEYLN
                     ETVPDEVITGLIKEEQYMSFIREGCGKIIKSL"
     gene            3483..4112
                     /locus_tag="PKH42_09095"
     CDS             3483..4112
                     /locus_tag="PKH42_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOA20630.1"
                     /translation="MKYKYVIFDFDGTLADTEEANFVIYQKLAEKYNLRNITMDELGR
                     LKKMHAKEVMAYIQLKKRYLPFLLKRGKTLLTQDIKNISLCKPDIFHIIGRLRNMGIK
                     TGIITTNSKTNVKLFLETHNVDVFDTIVSAAMFGKESKMKKIARKAKMSLDKFLYVGD
                     EIRDITAARNAGMDIASVGWGYNTVESLRKHEPDYLIFEPSELIDICKG"
     gene            4124..>4753
                     /locus_tag="PKH42_09100"
     CDS             4124..>4753
                     /locus_tag="PKH42_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOA20631.1"
                     /translation="MSRIYTIKAEYHDVENLTKILSEHNEIQFVSFMGVDIGGNGTDE
                     KIPVKLFIKDMEEMLKYGIQTDGSSVELQGIADLSDARVDLLPDTDCTWFVDYNYEYC
                     NDENGLPVGTLIIPSFIVHKNKLVCSRSTLRRAEVHFKNEIKNVLKNHPHIAESLGFK
                     YEDIDEVLLTAATELEMWVKTPEQSADFEELHASQVLKEQYWKRTMGTVR"
BASE COUNT         1797 a          669 c          965 g         1322 t
ORIGIN      
        1 tgatgtgttt tttgtttgtt atagcccgac atttcttcat aacaattgaa attttcatgc
       61 atatactaat gagagaacag aaaggagaat atttatgata attcatgttg tacagcccgg
      121 agaaacaata tattccattg caaatcaata caatatatca ccgcaaaaaa taattataga
      181 taacgaactt gaaaacccta atgtccttgt acccggccag accttagtaa tacttattcc
      241 ggatgtaatt catgttgtac agccgggaga aacattggca gatatagcaa ggcaatacaa
      301 tacaaccacc gtaaatttac tgcagcttaa tccctttatt gaatatgcag atcaaattgt
      361 gccaggtgac caaattacaa taagcaatac agcagataaa agaggtctca tacatgtgct
      421 tggatatgct tatccgcata tagacaggac agtacttttg aaaacacttc cttacttaac
      481 ttatctgacg atatttactt acggaatcac tgaagaaggc gaattaatcg atatagaaga
      541 cgaagacata atcagaatgg caagagattt tggtgtagca ccgcttatgc ttatttccac
      601 attgactgaa gaagggactt tcagcaatga actggcacat gttattttaa atgatccggt
      661 tgcacaaaac aatttaattg aaaatgtatt gcagaattta agagaaaaag gttattacgg
      721 actagacata gattttgaat atgtcctgcc ggaggacgaa gaagctttta taaattttat
      781 tcaaaatgtt catgacagaa taagccctga aggatttgtt ttatttacgg cgttggcacc
      841 aaaaacatca gcagaccagc cgggacttct ctatgaagct cataattatc cgagaatcgg
      901 agaagcgtca gatagagttc ttcttatgac ttatgaatgg ggttatactt atggtcctcc
      961 aatggctgta tctcccgttg acagggtaag ggaagtgtta gattatgcag tgactgaaat
     1021 cccgagggaa aaaatttata tgggcattcc taactacggt tatgatttta tactgccata
     1081 tgttcaaggt gtatcaagag cggactccgt ttccaatgtt gaggcagttg atttggcagc
     1141 tgatgttgga gcatacattc agtatgatga agtatcacaa gctccctact ttatttatta
     1201 tgatgaacag ggaaggcagc atagagtttg gtttgaagat gcaagaagca tcttggcaaa
     1261 attggattta ttcagtgaat acgggtttga aggtgtcggg tattggaacg taatgagata
     1321 tttcccgcag aactggcttt tagtgagcag tttatatgat attgccaagc ttgtttgatt
     1381 aaagcttgcc atatctgtaa ttatacaaaa aaatcagcat ataaataaat aacagagata
     1441 ttaggaggtt tttatgaaca ataaaaatta tagagtgttg aagttttttg agccggtaaa
     1501 taaaaaagac aagtctagag gctatgtacg tctggacatg agagggaaca ggggaaacat
     1561 gattgtagct gctgaaaatt taggtgatgc aaaatcatca agtgaagttt atttatataa
     1621 ggataaacac aacaaaatca aattaggaga tattaacaat aaaaaaggta taatcaaaaa
     1681 gaagctcacc tttgaaaaca gcaatgcaat tgaagattac aacgtatgcg ccgttgtaaa
     1741 ggatggaaaa attgttttat attccagttt gttcagcgta tcaagccaag acatcgtgag
     1801 aaagttggaa gaagatgata atgagcagga aaaagatatt tttgccgata agaaacaaaa
     1861 atttcaagag tcaaagccta tccatactaa agaaaaagag cagataaata taaaagatac
     1921 aaggggagcg atagaaccaa aagaagtaat tgagacaaaa gaaataatcg agccaaaagg
     1981 attacgagag gtaagggaaa taagagagaa gggaatggca aaagcagcag aaaataaacc
     2041 ggaagatgaa acaaaagaag aagtaaaaaa agtagtacaa gaaccagtaa aaacaataaa
     2101 agttaaagaa acaataaaag aagatagtga agaaaccatg gagactccgg aaaaatcaag
     2161 caaaaaagat gatatttcca atcagggtta tagaaaccgt tttgatgaga aattgtataa
     2221 aacattgaga gaatttaaag aaattgaacc tttatcggtt aaattaaaga attttaattg
     2281 gtggtatata ccttatgacg aaatgggcat aaaaacagga gtattgcctt attacaatca
     2341 aataataagc gcatattatc cgtatcccat gtctaatcgc gtgactacct gcacaagtct
     2401 aatgaaaaaa tacgggcatt atattttcgg catctacaag gaaaacgacg agattgcacg
     2461 gtttatatac ggggtgcccg gagaatttac aaaggaagag cagccttata aaggtgtgac
     2521 cggatttaaa aattggtcat atgcaaataa agacaataaa ggagaaagcg gatactggct
     2581 tgcttttgta aatccaaaaa caggtgaaat cacagacccg ccgcaagttt cattaactta
     2641 attctgtaca tcttgatttg tgtttgatat aatagtctag gaagagggat acaatctact
     2701 caagggaaag tcttcgatgg ccgaaggaat atccctgtag cgagggggac ttaccgacag
     2761 aatatccccg ctgcgattaa caattgaaaa ggagtaaaaa gtacaaatgc cgcctatcag
     2821 taccgtaatg cactttggga aaaagtttat ggaaagttac gataaagttg atatacctaa
     2881 ctttattata gggataattt taccaagtac attaaatgat gaccagttag aagagctgca
     2941 caatgttgat gaagacggga atatagatgt aagggagttt tatgagaaat ttgattttaa
     3001 aaccatgagc ttaaaggaca gctccattgt cttaggttat tattgccatc tttggctcga
     3061 tgagtattac aaatttaatg caagcaagct taaaatacat aataagcaaa atttaaaagg
     3121 tgaagaatta aactctgcag ttaaaaatct tttaaactat tatgacaaaa aagctatcgg
     3181 cagtttttat gaaaaatata cagaagatat aaaggctgtc caatcttata tatttgcagc
     3241 aagcaatata gataactcca agaaaaagtt gttggaatat ctaaatgaaa cagtacctga
     3301 tgaagtaata acaggcctta ttaaagagga gcagtacatg agttttatca gggaaggctg
     3361 cggcaaaata attaaatcat tgtaaaaaac tccgaaagga gtttttttaa ttttaaattg
     3421 actgattaaa aaaaatataa tatagtaata aataaaattt atatacttaa ggatgggtgt
     3481 cagtgaaata taaatatgtt atttttgatt ttgatggaac attagctgat actgaagaag
     3541 caaatttcgt tatatatcaa aagttagcag aaaaatataa tttaagaaat attaccatgg
     3601 atgaattagg ccgtctgaaa aagatgcatg ctaaggaagt tatggcatat attcaattaa
     3661 aaaaaagata tctgcctttt ctgttaaaga ggggaaaaac cttattaact caagatatta
     3721 agaatataag tctttgtaag cctgatattt ttcatattat aggcagacta agaaatatgg
     3781 gcataaaaac gggaattatt acaactaatt ctaaaacaaa tgttaagctt tttttagaaa
     3841 cgcacaatgt tgatgttttt gatacaattg tcagcgctgc catgtttgga aaagaatcaa
     3901 aaatgaagaa aatagcaaga aaagcaaaaa tgagtctgga taaatttctt tatgttggag
     3961 atgaaataag agatattact gctgcaagaa atgcaggaat ggatatagca tcggtaggat
     4021 ggggttataa taccgttgaa agccttagaa aacatgaacc tgattattta atatttgagc
     4081 cctctgagct tatagatata tgcaagggct agaaggagag tatatgagca gaatttatac
     4141 aataaaagct gaatatcacg atgttgaaaa tttgaccaaa attttaagcg agcataatga
     4201 aatacaattt gtatccttta tgggtgtcga tataggcgga aacggaacag acgaaaaaat
     4261 acctgtaaaa ctgtttatta aggatatgga agaaatgctt aaatacggca tacagacaga
     4321 cggctccagc gttgagctgc agggtatagc tgatttaagc gatgcaaggg ttgatttgct
     4381 gccggataca gactgtacat ggtttgttga ctataattat gaatattgta atgatgaaaa
     4441 tggtcttcct gtcggaactt taataattcc ttcttttatt gtccacaaaa acaaattagt
     4501 gtgctccagg tcaacactta gaagagccga agttcatttc aaaaatgaaa taaaaaatgt
     4561 tttgaaaaat catccccata ttgcagaatc cttagggttt aaatatgaag acattgatga
     4621 ggttttgcta actgctgcca ccgaattgga aatgtgggtg aaaacaccgg aacaaagtgc
     4681 cgattttgaa gaacttcatg catcccaggt attaaaagag caatattgga aaagaactat
     4741 gggtactgtc aga
//
LOCUS       DAOPUE010000136         4693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_18362_length_4693_cov_8.673566, whole genome shotgun sequence.
ACCESSION   DAOPUE010000136 DAOPUE010000000
VERSION     DAOPUE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18362_length_4693_cov_8.673566"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKH42_09105"
     CDS             <1..512
                     /locus_tag="PKH42_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20632.1"
                     /translation="PAAKNQAASTATTVAGLKDNFNELLLKLKDVGYMETDTWNVSVA
                     KISTPSGEELIANQSKVTDISIEDDVITVSVDVDELVAFPSSNLEQGTHKWIGMNITT
                     GLPDITAVKYNGYQLTSDDVTEAASVGGSAGDIVMWLKCDEIIDTPKIFTLWASGYSE
                     TTCMVIIEN"
     gene            532..840
                     /locus_tag="PKH42_09110"
     CDS             532..840
                     /locus_tag="PKH42_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004400079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HOA20633.1"
                     /translation="MTLLEKVKANLILEHSEDDELLQLYITAAVKYAESYQHLSENFY
                     ADNQMPPTTEQAVIMLSSHFYESRDGSTGGFYSDNVQAAEQVWNTVNLLLRLDRDWKV
                     "
     gene            842..1174
                     /locus_tag="PKH42_09115"
     CDS             842..1174
                     /locus_tag="PKH42_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HOA20634.1"
                     /translation="MSFGKMNTFIDILQKVTVKDSEGFSTEADVIIVSVRAYREKRHG
                     TEKWANRATFSVATDLFRFRCIPGATVTPDMIIVTKDERFEIISVENIKNRKMYIEVL
                     AKEVKPSG"
     gene            1167..1598
                     /locus_tag="PKH42_09120"
     CDS             1167..1598
                     /locus_tag="PKH42_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HOA20635.1"
                     /translation="MAKAVMKMPEDFLLKISKLGDKTDEIIPKVLEAGGKVVKEKVKS
                     NLQSIIGTNIKGKSRSTGELVSALGVSPALIDKNGNSNVKVGFSEPRSDGKSNSMIAG
                     VLEYGKSGQPPKPFLKPAKSASRKACINAMIEAFDKEVENL"
     gene            1595..1933
                     /locus_tag="PKH42_09125"
     CDS             1595..1933
                     /locus_tag="PKH42_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20636.1"
                     /translation="MSMLEELITLISPLVPIETGVFSDTPPNKYMVITPLVDTFELHG
                     DNEPGYEIQEARLSLFVKGNYTQLKKTIVCSLLSADFTITDRRYIGHEDDTGFHHYAI
                     DVAKLYKLEG"
     gene            1936..2526
                     /locus_tag="PKH42_09130"
     CDS             1936..2526
                     /locus_tag="PKH42_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOA20637.1"
                     /translation="MATIGLDSLYYSKITEDTDEIETYAEPVKLAKAIKADLSIELAE
                     AILYADDGAAVVVKEFKNGKLSLGIDDIGSTAAGDLTGAKIDDNKVLISTSEDGGEPV
                     AIGFRAKKANGKYRYFWLYKVKFGIPATNLQTKGDSITFQTPTIEGTVMRRNKVDDND
                     NHPWKAEVNEDDTGVIASTITSWFTEVYEPEFTVTP"
     gene            2540..2926
                     /locus_tag="PKH42_09135"
     CDS             2540..2926
                     /locus_tag="PKH42_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20638.1"
                     /translation="MNDERSSKITIGEVDYTLILTTRATKEIAKRYGGLENLGEKLMK
                     SENFEMALDEIVWLITLLANQSLLIHNLQNKDDIKELLKEEDVEILTSPLELSTYKGA
                     IIEAMFKGTKRYIESEETESKNEVVE"
     gene            3231..3644
                     /locus_tag="PKH42_09140"
     CDS             3231..3644
                     /locus_tag="PKH42_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20639.1"
                     /translation="MSINFETLIPYNKILESPEDVLDLVDENGQVILLKNNEPSYLII
                     RAKDAIQLTKLNEYNQYKETKYKLHEAMKIVLSETDDKIMHASDLADEIYLRGLYLKK
                     DGTKAEYNQIRARCGHYPSLFEALPGNIIKLKAKI"
     gene            3674..3991
                     /locus_tag="PKH42_09145"
     CDS             3674..3991
                     /locus_tag="PKH42_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20640.1"
                     /translation="MNQNFICPFPIRWNEIFKDLCKAYETLTGIKLPSKVSDVHSAGG
                     PPTPLILAGWVFSNDNDKQERWQETLQWAEKYNLLQLTTVEEHDKCFSAGNTRSGGIP
                     FGE"
     gene            4033..4548
                     /locus_tag="PKH42_09150"
     CDS             4033..4548
                     /locus_tag="PKH42_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20641.1"
                     /translation="MEFDDNFKNLSDLDDETLDSSGLYCIRLKENSKLPDRYQNILDS
                     RKIKYIYIGKATTTLRSRMKEELEHIGPGTFFRSIGCALGYKPMHGHLIGLANQNNYR
                     FSQDDKMKIIDWLNDNIEVSIVKYEGDFNIEANLIKQYCPLLNITHNPKKLQELKEDR
                     QNCKRIARGEH"
BASE COUNT         1671 a          709 c          976 g         1337 t
ORIGIN      
        1 ttcctgcagc taaaaaccaa gctgcaagca ctgcaactac tgtagcaggt ttaaaagata
       61 actttaacga acttcttcta aaacttaaag acgtaggata tatggaaaca gatacatgga
      121 atgtttctgt agcaaaaatt tcgacaccct ccggtgaaga acttattgca aatcaaagca
      181 aggttacaga tatttcaatt gaagatgacg ttattactgt gtctgtagat gttgatgaac
      241 ttgtagcatt ccccagttcc aatctggaac aaggtacaca taagtggatt ggcatgaata
      301 ttacaacagg gctacctgat attacagcag taaaatacaa cggttatcag cttacatctg
      361 atgatgtaac agaggctgct tcagttggag gatctgccgg cgatattgta atgtggctta
      421 agtgtgatga aattattgat acaccaaaaa tcttcactct atgggcttct gggtatagcg
      481 aaaccacttg tatggttatt atagaaaact gatgaaaggc ggtggttaat aatgacacta
      541 ttagaaaaag tcaaagcaaa tcttattctc gagcacagtg aagatgatga actcttgcag
      601 ttatatatta ccgccgctgt aaagtacgct gagagctatc agcatctttc agaaaacttt
      661 tatgcagata atcaaatgcc tccaactact gaacaagcgg tgattatgtt gtcttcccac
      721 ttctatgaat caagggatgg gagcactgga ggtttttact cagataatgt tcaagccgca
      781 gagcaggtat ggaatacggt aaacctgctc ttacggttgg acagagattg gaaggtgtag
      841 tatgagcttt ggtaaaatga atacctttat tgacatatta cagaaggtaa ctgtcaaaga
      901 ttctgagggt ttttctactg aagctgacgt tattattgtt tcagttcgag cttatagaga
      961 gaaacgtcat ggaactgaga agtgggctaa cagagcaacc ttctcagttg ctaccgacct
     1021 ttttagattt cgatgtattc ccggtgctac agttacacct gatatgatta ttgtaacaaa
     1081 agatgaacgt tttgagataa tttcggttga gaatattaaa aatcgaaaaa tgtatattga
     1141 ggtactcgca aaggaggtga agccaagtgg ctaaggctgt tatgaaaatg cctgaagatt
     1201 ttcttctaaa gatttcaaag cttggtgata agacagatga aataattcct aaggtccttg
     1261 aagccggtgg caaagttgta aaagagaagg ttaagtctaa tttacaaagt attattggca
     1321 ctaatataaa aggaaaaagt cgttccaccg gggaacttgt ttctgccctt ggtgtctccc
     1381 ctgctcttat tgataagaat ggtaattcta atgtcaaagt tggtttttcc gagcctcgct
     1441 ctgatggaaa aagcaattca atgattgccg gtgttttgga atacggtaaa agcgggcagc
     1501 cacctaaacc ctttctaaaa cccgctaaat cagcaagtag gaaggcatgt attaatgcca
     1561 tgatagaggc ctttgacaag gaggtagaaa acttatgagt atgttggaag aattaattac
     1621 ccttatttca cctttagttc caatagagac aggagtgttt tcagatactc cccctaataa
     1681 atatatggtt attactcccc ttgtagatac ttttgagctg catggagata atgaaccggg
     1741 atatgaaata caggaagcaa ggctgtccct ttttgttaaa ggcaattata cacaacttaa
     1801 aaaaacaatt gtctgctctc tattgagtgc agactttacc ataacagacc gccggtacat
     1861 cggtcatgaa gatgataccg gctttcacca ttatgccatt gatgtggcaa aattatataa
     1921 attggaggga taaaaatggc aacaatagga cttgatagtt tatattattc aaagattaca
     1981 gaagacactg acgagataga aacatatgca gagccggtaa aacttgcaaa agcaataaaa
     2041 gctgatttat caatcgaact tgcggaggca attctttatg cagatgatgg tgctgctgtt
     2101 gtagttaagg aatttaaaaa tggcaaactt tcacttggaa ttgatgatat tggttctact
     2161 gctgcaggtg atttgaccgg ggctaagatt gatgataata aggtgttaat atctacaagt
     2221 gaagatggag gtgagcctgt tgcaatagga ttcagggcaa agaaagctaa cggaaaatac
     2281 agatatttct ggctttacaa ggttaaattc ggaatacctg caactaatct tcagactaag
     2341 ggagacagca taacctttca gacacctacc attgaaggca ctgttatgag aagaaataaa
     2401 gtagatgata atgataatca tccatggaaa gcagaagtaa atgaggacga tacaggagtt
     2461 atagcatcaa ctataaccag ctggtttact gaagtttatg agcctgaatt tactgttaca
     2521 ccataggagg tattgtaaaa tgaatgatga aagaagcagc aagattacaa taggtgaagt
     2581 tgactatacc cttatattga ctacaagagc tactaaggaa attgctaaaa ggtatggagg
     2641 tcttgaaaat cttggtgaaa aacttatgaa atcagagaac tttgaaatgg ctcttgacga
     2701 gattgtatgg cttatcacct tacttgcaaa tcagagtttg ttgattcata atctgcagaa
     2761 taaggatgat ataaaagaac tccttaagga agaggatgtt gagatactta cctctccact
     2821 tgaattatca acatacaaag gagctattat tgaggcaatg tttaaaggaa ctaaaagata
     2881 tattgagtca gaggaaactg aatcaaaaaa cgaggtagtc gagtaagcga tgaagagttg
     2941 tttgctcgac tcatttatta tggagttaca cagctttgta ggaatgagga ggatgtgtgg
     3001 cttatggcaa taggagatct cttggatcag tgggaaatac acaaacagtt tatgggattg
     3061 gctaagccta aagttgagtt gtttattgat gaagtgatac ctattggtat ctaacactta
     3121 atacaccaaa attcatacaa aaataacata tttgtatgaa tttgtttgac atttgtttac
     3181 gaatgtgtta ttgtaatttt agaaaaatac gagatttttg gggtgaaaag atgtcaatta
     3241 attttgaaac attgattcct tataataaaa tattggaatc accggaagat gtgcttgatt
     3301 tagtcgatga aaatggacaa gtgatattac ttaaaaataa tgaaccttct taccttatta
     3361 ttagagcaaa agatgcaata caattaacta aactaaatga atacaatcaa tataaagaaa
     3421 ccaaatataa gctgcatgag gctatgaaga ttgtattgag cgaaacagat gataaaataa
     3481 tgcatgcgtc agacctcgcg gatgaaatct atttaagagg attgtattta aaaaaagatg
     3541 gtactaaagc cgaatacaat cagattagag caagatgtgg ccattatcct agtctatttg
     3601 aagcccttcc aggcaacatc ataaaactta aagctaaaat ttgaaataat aattcagtca
     3661 aggagaaagt acgatgaatc aaaattttat ttgtccattt cccattaggt ggaatgaaat
     3721 atttaaagat ttatgcaaag catatgagac tttaacagga ataaaactgc catctaaagt
     3781 ttctgatgtg catagtgcag gtggtccacc aactccatta atactagctg gatgggtgtt
     3841 ttcaaacgat aatgacaaac aggagcgatg gcaggaaaca ctacaatggg cagaaaaata
     3901 taaccttctt caattaacga cagtcgaaga acatgacaaa tgtttttcag caggaaatac
     3961 acgaagcgga ggcatacctt ttggcgaata atatacacat attaacaaaa taatatcaag
     4021 gagaatgcta ttatggaatt tgatgataat tttaaaaatc tttcagattt agatgatgaa
     4081 actttggaca gcagtgggct atattgcata agattaaaag aaaactcaaa gcttccggat
     4141 agataccaaa atattcttga tagtaggaaa attaagtata tttatattgg caaggcaact
     4201 acaaccttaa gaagtcgtat gaaagaagaa cttgaacaca ttggaccggg aacttttttt
     4261 agaagcattg gttgtgcatt aggctacaaa ccaatgcatg ggcatttgat aggattggct
     4321 aaccagaata attatagatt ttcccaagat gataagatga aaataataga ttggttgaat
     4381 gataatattg aagtcagcat agtaaaatat gaaggtgatt tcaatataga agcaaatttg
     4441 ataaaacaat actgtccatt attaaatatt acacataacc ctaaaaaact acaagaactc
     4501 aaggaagaca gacaaaattg taaaagaata gcaagaggag aacattaatt atttaattat
     4561 ctattgcata ataacaagtc aatggcactc taacaagggt gtctttttta tgctcaaaat
     4621 aaggaggtga aagcatggca gacaatttcg gactcaagat tggtgttgaa ggcgaacgag
     4681 agtttaagaa agc
//
LOCUS       DAOPUE010000137         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_18385_length_4688_cov_14.027412, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000137 DAOPUE010000000
VERSION     DAOPUE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18385_length_4688_cov_14.027412"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..641)
                     /locus_tag="PKH42_09155"
     CDS             complement(213..641)
                     /locus_tag="PKH42_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20642.1"
                     /translation="MDIEVFVKRVYTVSFRLTGQEQVAEEIASQAIIHTFKEMNEDYK
                     EKVTENMFHLTIMELVKIFLNNPNSQCNDNLKGIKRALLELKPINRAVVIWKDVLGYK
                     IHDNIPVSDYTYEELYEELVCGRKELKEYKILRASGEQFS"
     gene            complement(731..1414)
                     /locus_tag="PKH42_09160"
     CDS             complement(731..1414)
                     /locus_tag="PKH42_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOA20643.1"
                     /translation="MKRILIIEDDKSISELQKDYLEMSGYEVLCVFDGETGLEHIIND
                     DFDMVILDLMLPQKDGFDILKEISDSKQIPVLIVSAKSDEMYKIKGLNLGADDYITKP
                     FSMGELVARVNSHLKTYERFNNNSNKKQISAGAISIDKEDRRVYLENNEVFLTQKEFD
                     LLLFLVENPNRVYSKDELYEKVWGYDALSDTTTITVHIARIREKIEIYPNGHQYIETV
                     WGAGYRFKI"
     gene            complement(1416..2735)
                     /locus_tag="PKH42_09165"
     CDS             complement(1416..2735)
                     /locus_tag="PKH42_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOA20644.1"
                     /translation="MNLKQRFLKQSILTLIITIIITGCVGFVYDYFYNLLNKSSVYVG
                     LGKTATVVIENDNIIYNDEEFSSLQIKEMMMNISVESNYYEHDNTKFILDTETFTTVS
                     GDEFMIITLNAVTDASDYYRSLMLLVFIIFLIVFIVTNIVVQKQNMINIINPIINLTK
                     ETERLRTGELETAIIEQGYGEVRELGSAVEQLRLQLKNSIYYQQKFDENRKFLISSIS
                     HDLKTPVTSIRGYLDGVLDGVADTDEKKKYYLSKAVDKTKLINNMIEDLLLYSKLDLN
                     QMPFEKDRINIVKYIESCLEDNSAEFERENKGIILDNKLSDEYLVNIDREKFKRVIQN
                     IVDNAKRNIKKEFGKLTIILRETNSSVIMEFKDNGKGISKDDLPYVFDKFYRADTARA
                     VEGSSGLGLAIAKQIVEGLGGRIWAISEIDEGTSIIISLKKAIERKW"
     gene            complement(2743..3486)
                     /locus_tag="PKH42_09170"
     CDS             complement(2743..3486)
                     /locus_tag="PKH42_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20645.1"
                     /translation="MEPLKAVYINELFKISKKKKIKAALIFSILSVIVAAIAVYFLNN
                     FAGIRITGSSDFSIMVLTVLSYTLFPLFTAFICIDMFAGEFADDTIKFTLTGPAPRFK
                     VFSGKILAIAGFIIVNLIFVMILSLIISIFINGNMTNVLKILLAYIMEFFPQLIFALT
                     VVFISNITRGTTSAFMLSILIFLLFNGLGFIFPSYKSLFFTSAFDWYTLILGGYINFS
                     KILRVFLILLGYGIMLYTASYYLFEKKEV"
     gene            complement(3473..4198)
                     /locus_tag="PKH42_09175"
     CDS             complement(3473..4198)
                     /locus_tag="PKH42_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20646.1"
                     /translation="MDKAIVIENLSKTYKNSRGIQDINLEIQRGEIFGFLGPNGSGKS
                     TTMKVMVGLMKADKGDVRINGYSISKDYEQAMKRVGCIIENVAPFPYLTAYENMKLCG
                     RFHENVDEGRIDECLDITGLLKYKNEKIKNYSLGMKQRIGIAMAILSNPEVLILDEPL
                     NGLDVKAMVEMRKLFLNLSNQHKKTFFISSHLIHDIELTCSKVGILYDGKLLGVDSTQ
                     NILKNYSSLENYYLSEVDLNGAS"
     gene            complement(4258..>4688)
                     /locus_tag="PKH42_09180"
     CDS             complement(4258..>4688)
                     /locus_tag="PKH42_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20647.1"
                     /translation="LDGQKVTYSKEGFEFDEKYIGKYKNNIVKVEDNSFVKIGERIIE
                     ITSVEDGNVKGRYYEVYSEEYEPDIVRNFEFFSNHDDSEFYTILNYTNNNGEKKQGVM
                     HRTNLQDLYVSFNITFDEDGSGYSAANIDDFNSEFVRVFE"
BASE COUNT         1481 a          891 c          575 g         1741 t
ORIGIN      
        1 atgataccgg ctcatttgct tcccaaatta tcatcattat ttccaattca ctgtcaggaa
       61 ttcttttaat agtatccatt tccaccacct cacatcctct caaaataaat ttaaattata
      121 ttattaaact cattctacga ttgtagaatg agtttgtcaa ggattatttc agaaacatta
      181 aactttagat tgtcaattcg catttttcct cttcaactaa attgctctcc agaagcccta
      241 agaattttat attccttcag ctcttttcta ccgcaaacaa gttcttcata taactcttca
      301 taagtgtaat cagatacagg aatgttatca tgtattttat agcctaatac atccttccaa
      361 ataactactg ctctgtttat aggtttgagt tctaatagag cccttttaat tccttttaag
      421 ttatcgttgc attgcgaatt cggattgttt aagaatatct ttaccaactc cataattgtc
      481 aagtgaaaca tgttttctgt tactttttct ttataatctt cattcatttc tttaaaagta
      541 tgtattattg cctgagaagc tatttcttct gcaacctgct cttggccggt aagcctgaat
      601 gaaactgtat atactctttt aacaaatact tcaatatcca taactccccc acctatatta
      661 acaaatcctc atattaaaaa aattgctaga ctataaatta aaaggcaaat aaaatcatat
      721 gttatttata ctaaatttta aatctgtatc ctgctcccca tacagtttca atatattgat
      781 gtccattagg atatatttcg attttttctc taattcttgc tatatgaaca gttattgttg
      841 ttgtatctga caaagcatca taaccccaaa ccttttcgta taactcgtcc ttgctataaa
      901 ccctatttgg attttctact aaaaataaaa gcaaatcaaa ttccttttga gtcaaaaaaa
      961 cttcattgtt ttctaaataa actctacggt cttctttatc gatagatatg gctcctgcac
     1021 ttatttgttt cttatttgaa ttgttattaa acctttcata tgtttttaag tgactgttta
     1081 cccgtgcaac taattcaccc atgctaaagg gtttagttat gtaatcatct gctcctaagt
     1141 ttaatccttt aattttatac atttcatcag attttgctga cacaataagc accggtattt
     1201 gttttgaatc ggatatttct tttaatatat caaagccatc cttttgagga agcatcaaat
     1261 ctaaaataac catatcgaag tcatcattta ttatatgctc aagcccggtt tcgccgtcaa
     1321 aaacacatag gacctcataa ccgctcattt ctaaataatc tttttgcagc tctgaaatac
     1381 ttttatcatc ttcaataatt aatatccttt tcaatttacc acttcctctc aatagccttt
     1441 tttaatgaaa taataatgga tgttccttca tcaatttcag aaattgccca aattcgtccg
     1501 cctaaacctt ctactatttg ttttgcaatt gccagaccca aaccacttga accttcaact
     1561 gctcttgctg tgtctgccct gtaaaactta tcaaaaacat aaggcaaatc atccttactg
     1621 attcctttgc cgttatcctt aaactccatt attactgaag aatttgtttc tcttagtata
     1681 attgtaagtt ttccaaattc tttttttata tttcttttgg cattatcaac tatattttgt
     1741 atcacacgtt taaatttttc cctatctata ttaacgagat attcatctga aagcttatta
     1801 tctaaaataa ttcccttgtt ttcacgttca aattccgctg aattatcctc aaggcagctt
     1861 tctatgtatt ttacaatgtt aatcctatct ttttcgaatg gcatttgatt taaatcaagc
     1921 ttggaataca gcaataaatc ctcaatcatg ttgtttatga gctttgtttt gtcaacggct
     1981 tttgataaat aatatttctt cttttcatca gtatcggcaa caccgtctaa tactccgtct
     2041 aaatatcctc ttatggatgt aacaggagtt ttaaggtcat gtgatatgct tgatattaaa
     2101 aattttctat tttcatcaaa cttctgctga taatatatag agttttttaa ctgaaggcgc
     2161 aattgctcta cagcacttcc taactctctg acttcaccat aaccttgctc tataattgct
     2221 gtttcaagct ccccggttct caacctttcc gtttcctttg tcaaatttat aataggattt
     2281 atgatattta tcatgttttg cttttgtaca actatgtttg ttacaataaa aactattaaa
     2341 aatattataa aaactaatag cataagtgac ctgtagtaat cacttgcatc tgtgactgca
     2401 ttgagagtta taatcataaa ttcatcgccg ctaactgttg taaaagtttc tgtatccaag
     2461 ataaatttag tattatcgtg ttcataataa ttactttcaa cacttatatt catcatcatt
     2521 tcttttattt gcaggctgct gaattcttcg tcattatata tgatattatc attttcaatg
     2581 acgacagtgg ctgtttttcc caaaccaaca taaacagaag atttatttaa taaattgtaa
     2641 aaataatcat agacaaagcc gacacatccg gtaataatta ttgtgattat aagtgttaga
     2701 atgctttgtt ttaaaaacct ttgttttaaa ttcatagtat acctaaactt cctttttttc
     2761 aaaaagatag tagcttgctg tatataacat tataccataa ccgagcaaaa ttagaaatac
     2821 tctcagtatt ttgctaaaat ttatatagcc tcctaaaatc aatgtgtacc aatcaaaagc
     2881 agatgtgaaa aataaacttt tgtaagatgg aaaaataaat cccaatccat taaacaataa
     2941 aaatataagt atagaaagca taaaggcgct tgttgttccc cttgtaatat ttgaaatgaa
     3001 aacaactgtt aatgcaaaaa taagctgcgg gaaaaattcc ataatatatg ctaacaatat
     3061 ttttaaaaca tttgtcatat taccatttat aaatatagag attattaagg ataaaatcat
     3121 tacaaaaata agatttacta ttataaagcc ggcaattgca agaatttttc ccgagaatac
     3181 tttaaatctc ggcgcaggcc ctgtcaaggt aaatttaata gtatcatctg caaattctcc
     3241 ggcaaacatg tctatacata taaaagcagt aaaaagaggg aaaagtgtat agcttaatac
     3301 agttaaaacc atgatggaaa agtcagagct tcctgtgata cgtatccctg caaagttatt
     3361 taaaaaatat actgcaatgg ccgcaactat gaccgataga attgaaaata tcaaggctgc
     3421 tttaattttc ttcttttttg atattttgaa taattcattt atgtacactg ctttaagagg
     3481 ctccatttag atcaacctca cttaaataat agttttccaa ggaagaataa ttttttaaaa
     3541 tgttttgtgt gctgtccact cctaataatt taccatcata taaaatgcct actttgctgc
     3601 acgtaagttc tatatcatgt attaaatgac tggatataaa aaatgttttt ttgtgctgat
     3661 ttgacaggtt aagaaataat tttctcattt ctaccatagc tttaacatct aaaccattta
     3721 gaggctcgtc taatatcaaa acctcaggat ttgataaaat ggccatggct atacctattc
     3781 tctgcttcat acctaaggaa tagttcttta tcttctcgtt tttatattta agaagtcctg
     3841 taatatccaa gcattcatca attcgtcctt catcaacatt ttcatgaaat ctcccgcaca
     3901 atttcatatt ttcatatgcg gtcaaataag gaaagggggc tacattctct ataatacaac
     3961 caaccctttt cattgcctgt tcataatcct tggaaatact gtaaccattt atcctgacat
     4021 cgcctttgtc tgctttcatg aggccgacca tgactttcat tgtagtgctc ttccctgaac
     4081 cattaggacc taaaaatccg aatatttcac cacgttgaat ttccaaattg atgtcttgta
     4141 ttccgcggct gtttttatag gtttttgaaa gattttcaat tacaatagct ttatccaaca
     4201 tatcctcctt aaaaaattgc tttgaggcgc aaagacccaa gccttgcgcc tgcattgcta
     4261 ttcaaatact cttacaaatt cgctattgaa gtcatcaata ttagcagctg aatatccact
     4321 gccatcttca tcaaatgtaa tattaaatga tacatatagg tcttgtaaat ttgtacggtg
     4381 cataactccc tgtttctttt cgccattgtt gtttgtatag tttaatattg tgtaaaattc
     4441 tgaatcgtca tgatttgaaa agaattcaaa gtttcttaca atatccggtt catactcttc
     4501 actatatacc tcataatatc tgccctttac attgccatct tcaacagaag ttatttctat
     4561 aattctttcg cctattttta caaaggaatt gtcctctacc tttacaatat tatttttata
     4621 ttttccgata tatttctcat caaattcaaa gccttccttg ctgtaagtaa ccttttgtcc
     4681 atccaaat
//
LOCUS       DAOPUE010000138         4658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_18532_length_4658_cov_13.499240, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000138 DAOPUE010000000
VERSION     DAOPUE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18532_length_4658_cov_13.499240"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PKH42_09185"
     CDS             complement(<1..401)
                     /locus_tag="PKH42_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOA20648.1"
                     /translation="MKGNIMEIRNTTINDIDEVLKIFADARKFMKAAGNPNQWKDSHP
                     ERALIENDIANNIGYVCVDEGKITATFVYFEGMEPVYREIHQGRWLNDEPYGVVHRIA
                     SAEGKRGAASFCLNWCFEKCRNLKIDTHRDN"
     gene            complement(450..2402)
                     /locus_tag="PKH42_09190"
     CDS             complement(450..2402)
                     /locus_tag="PKH42_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOA20649.1"
                     /translation="MRSKKTAIILLVAMLITLFPAGAFAETMKAETVYINGNIYTVDE
                     DFSTAQAIAIKGQYIIGIGSNDEINAFIGESTEVIDLGGKTVIPGLIEGHMHYPGEGQ
                     KLTQLDVFWKPKDVILAAVKAEADRLPDGEWITGRGWNQEVWDVPEFPTKEDLDEVAP
                     NNPVALIRTCGHATWVNSLALEIGGVTKDTPNPQGGEILKNEEGELLGILTDTAASMV
                     RGKIPPLTVERQKEALLLAQEELFSYGLTSSMDAGSGVNDIKNMKDLYESGDLKIRLY
                     VMVDSGADAEAYYEIGPEVGLYDNRLTMNCIKFYTDGSLGARSAWMLEEYSDRPGHFG
                     NSRHTYDEFYELVKAARDNGFQASTHAIGDAANRQVIDIYEKVLKENPQEDHRYRIEH
                     FQIAALDDIQRIADLGIIPAMQGVHATSDKNMAEDRVGPERIKGGYAWRKVLNTGNII
                     VNGSDANVELVNPYHGLYASVTRTGRDGDPVGGWYPEECMTREEALRSFTIWAAFGQF
                     EEDIKGSLELGKLADFVVLDRDYMTCPERDIKDILPLMTVLGGEVVYEKDNSQVTVVY
                     EGMPLGFDSPTILENGRTFVLAKNLFNSFELDYEYDEAANKIKVNGIEVDARDKGTYV
                     PLRAVAEGLGYKVNWYQQSMSVSIGK"
     gene            2850..3806
                     /locus_tag="PKH42_09195"
     CDS             2850..3806
                     /locus_tag="PKH42_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOA20650.1"
                     /translation="MTKAKKAVYYICDNPDWGYVSYLVWDVLKEEGYLDNKVDFTFDG
                     REVYKYTDKNQNEFYFVPTEIALCRDYPRYLADMNKYFSDFDMSGMVTWHEGANALEK
                     VFTVHSIGDVNSGCYGPANPLYMHNIMWAMEKLKKEIGLDFSVVSEATHWSGVYEGQG
                     DPELLLEYPVPIVDIEIGSEPESWVNLDACRVLARSLLHVFDKDKDDIKLHNLLCVGG
                     VHFDPNFAEAVHTSWNENEFFGISHIIANQWLVSGEYEGEKGIEMASNCVDAIIGGID
                     GIVFHDKLKGCYKDVVRALGEKYNVPILKHQALRKPESISWR"
     gene            complement(3793..>4658)
                     /gene="galE"
                     /locus_tag="PKH42_09200"
     CDS             complement(3793..>4658)
                     /gene="galE"
                     /locus_tag="PKH42_09200"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015595889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOA20651.1"
                     /translation="IEAITNKRPEFYQTDLLDKPGLEKVFKENQIDCVIHFAGLKAVG
                     ESVEKPLVYFNNNISGTINLLMAMEKFSCKKIIFSSSATVYGKAEVMPLNENSPIGVT
                     NPYGRTKLVIEDMLKDIYASDNDKSIVLLRYFNPIGAHKSGLIGEDPNGIPNNLMPYI
                     CKVAKGELPVLNVFGNGYDTIDGTGVRDYIHVEDLAAGHVKAYGKIRTETGLYTYNLG
                     TGKGYSVLQVIDAFEKANSIKINYIIAPRRPGDVAEVYADTKKAETELGFKAQYTLED
                     MCRDSWNFIAN"
BASE COUNT         1326 a          993 c          813 g         1526 t
ORIGIN      
        1 atattgtcac ggtgggtatc gatcttcaaa ttcctgcact tctcaaagca ccaattcagg
       61 caaaaggatg cggcacccct tttaccttca gccgaagcaa ttctgtgaac aacaccgtaa
      121 ggctcatcat taagccatct gccctggtgt atttccctat agacaggctc cattccttca
      181 aaatacacaa aggttgctgt aatttttcct tcatcaacgc atacatagcc gatattattt
      241 gcaatatcat tttcaattaa tgctctctca gggtgactgt ccttccattg atttgggttt
      301 ccggcagcct tcataaattt tcttgcatct gcaaatattt ttaaaacttc atcaatatca
      361 tttattgtgg tgtttctaat ctccataata tttcctttca tttgaattaa gccacctgac
      421 taatcagatg gctttatatt caatctttac tatttcccta ttgaaacaga catactttgc
      481 tgataccagt ttaccttgta gcccagtcct tcagcaactg ctcttaaagg cacataagtc
      541 cccttgtctc ttgcatctac ttcaatgcca ttgaccttga tcttattagc agcttcatca
      601 tattcataat ctaattcaaa gctgttaaac agatttttag ccaatacaaa agtccttcca
      661 ttttcaagaa tagtcgggct gtcaaatccc aaaggcattc cttcataaac tactgttact
      721 tgtgaattgt ctttttcata aactacttct ccccctaaaa cagtcatcag tggaagtatg
      781 tctttaatat ctctttccgg gcaagtcatg tagtctctgt ctaatacaac aaagtcagct
      841 aatttcccta attccagaga acctttaatg tcctcttcaa attgtccgaa agctgcccaa
      901 attgtgaatg atctaagagc ttcttcacga gtcatgcatt cttcgggata ccatcctcca
      961 acaggatcac catctctgcc tgttcttgta acagaagcat agagaccgtg atatggatta
     1021 acaagctcaa catttgcgtc tgaaccatta actattatat tgcctgtatt cagaacctta
     1081 cgccatgcat agccgccctt aattctttca ggaccaactc tgtcttcagc catgtttttg
     1141 tctgaagtgg catgtactcc ctgcatagca ggaataattc ctaaatctgc tatcctttga
     1201 atatcatcca aagcagcaat ttggaagtgt tctattctgt atctgtgatc ttcctggggg
     1261 ttctctttca ataccttttc ataaatatca ataacctgcc tgttggccgc atctccgatt
     1321 gcatgtgtgc tggcctggaa accattgtct ctggctgctt taactaattc ataaaactca
     1381 tcatatgtat gtcttgaatt tccaaagtga ccgggcctgt cagaatattc ttccagcatc
     1441 caggcacttc ttgcacctaa tgagccgtct gtatagaact taatgcagtt catagtcagt
     1501 ctgttgtcgt aaagcccgac ttcaggacca atttcataat atgcttccgc atcagctccg
     1561 gagtctacca taacataaag ccttattttc aggtcgcctg attcatataa gtctttcata
     1621 ttcttaatat cattaacccc tgaacctgca tccattgaag atgtaagccc gtaggagaat
     1681 aattcttcct gtgccagcag caaagcttct ttttgtcttt ccactgtcaa tggaggaatt
     1741 ttgcctctta ccatacttgc tgcagtatca gttaatatac ctaaaagttc gccttcttca
     1801 ttctttaaga tttctccgcc ttgagggttc ggagtatcct ttgttactcc gcctatttca
     1861 agagctaatg aattaaccca tgttgcatga ccgcatgttc ttatcaatgc tacagggttg
     1921 tttggagcaa cttcatccaa gtcttccttt gtcgggaact caggtacatc ccatacttcc
     1981 tggttccatc cgcggcctgt aatccactct ccgtcaggca gcctgtctgc ttcagccttt
     2041 acggcagcta aaattacatc ctttggtttc caaaacacat ccaattgagt taacttctgt
     2101 ccttctccgg gatagtgcat atgcccttca attaaacccg gtataactgt tttgcctcct
     2161 aagtcaataa cctctgttga ctctccaata aacgcgttaa tctcatcatt gctgcctatg
     2221 ccgataatat actgtccttt aattgcaatg gcctgtgctg ttgaaaaatc ttcatcaaca
     2281 gtgtaaatat tgccgttaat atatacagtt tctgctttca tagtctctgc aaatgctccc
     2341 gcagggaata atgtgattaa cattgccact aataaaatga ttgctgtttt tttgctgcgc
     2401 atttgtatcc tccttttaat tatagattaa tataataaaa taccacatga gtattttatt
     2461 taaggtaaga aaagatatgt aatcaaagta tttatttaag ttaagaaaag atatgaaaat
     2521 aaagtatttt atttaaaagg caaaaaagtt ttaattaaat cgcagtatat cattaaaaac
     2581 cattacagtc tatatgtagt tttattcata attattaaaa acttttcatt ttttattttt
     2641 tacataattg gttatatttg gttaatacag gaccttgtct aataattgga tataatccct
     2701 tttaatcccg agtacgaaaa gaactatcac aaagcaattg gacatacccg gttatctgag
     2761 gacaaaaaga accgtctcca aaattttgtt gtctatttcc aaaaagatgt tataatactt
     2821 attaaattgt ttttagggag tgacaaaaaa tgacaaaagc taaaaaggcc gtttattaca
     2881 tctgcgacaa ccctgattgg ggttatgtca gttacctggt ttgggacgtg ctaaaagaag
     2941 aaggatactt ggataataag gttgacttta cttttgacgg gagggaagtc tacaaatata
     3001 cagataaaaa tcagaatgaa ttttattttg ttcctacaga aatagcatta tgcagagatt
     3061 atcccagata tctggcggat atgaacaaat atttttctga ttttgacatg tcaggaatgg
     3121 ttacatggca cgaaggtgca aatgcgctgg aaaaggtatt taccgtacat agtataggag
     3181 atgttaattc cggatgttac gggcctgcaa atcctttata tatgcacaat attatgtggg
     3241 ctatggaaaa attaaaaaag gaaatcggtt tggatttcag cgtggtatct gaagcaactc
     3301 attggtcagg agtttatgaa ggccaaggag accctgagct tctccttgaa tatcctgttc
     3361 ccatagtgga cattgaaata ggaagcgagc cggaaagctg ggtaaacctg gatgcctgca
     3421 gagttcttgc ccgttccctt cttcacgtat ttgataaaga caaggatgat ataaaacttc
     3481 ataatcttct ttgcgtggga ggagtgcatt ttgatccgaa ttttgcagaa gcagtccaca
     3541 catcctggaa cgagaatgaa ttcttcggca tatctcatat aattgcaaat cagtggcttg
     3601 tttcaggaga atacgaaggg gaaaaaggaa tagaaatggc atctaactgc gtagatgcca
     3661 taataggcgg aattgacggt attgtttttc acgacaaatt aaagggatgc tataaggatg
     3721 tggtccgtgc cttaggcgaa aaatataatg tacccatttt aaaacatcag gcattgagaa
     3781 aacccgaaag tatcagttgg cgataaaatt ccatgaatcc ctgcacatat cctcaagagt
     3841 atactgagct ttgaatccaa gctcagtttc tgcttttttt gtatccgcat atacttctgc
     3901 cacgtcgcct ggtctccggg gagcaattat ataatttatt ttaatactgt ttgccttttc
     3961 aaatgcatct ataacctgca atacgctgta accttttccg gtccccagat tatatgtgta
     4021 taagccggtt tccgtcctga ttttaccgta tgccttcaca tggcctgccg ccaggtcctc
     4081 aacatgaatg tagtctctca ctcctgtgcc gtctatagtg tcatagccat tgccgaaaac
     4141 attaagcacc ggcagttctc cttttgccac cttgcagata taaggcatta aattgtttgg
     4201 aatcccatta ggatcttcac ctatcaagcc gctcttgtga gctcctatgg ggttgaaata
     4261 ccggagaagc actatgcttt tatcattgtc cgaagcataa atatccttaa gcatatcttc
     4321 aataacaagc tttgtcctac cgtatgggtt tgtaactcca ataggcgaat tttcattaag
     4381 aggcataact tcagctttgc cgtagacagt tgccgaggag ctgaatatta ttttcttgca
     4441 gctgaatttt tccatagcca taagaagatt tatagttcct gaaatgttgt tattgaaata
     4501 cacaagaggt ttttccacgg attctcccac agccttcaat cctgcaaaat gaattacaca
     4561 gtcaatttgg ttttccttga acaccttttc caatcccggt ttatcaagca ggtctgtttg
     4621 ataaaattca ggtcttttat ttgttatggc ttcaattt
//
LOCUS       DAOPUE010000139         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_18870_length_4594_cov_9.753470, whole genome shotgun sequence.
ACCESSION   DAOPUE010000139 DAOPUE010000000
VERSION     DAOPUE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18870_length_4594_cov_9.753470"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKH42_09205"
     CDS             complement(<1..349)
                     /locus_tag="PKH42_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOA20652.1"
                     /translation="MISINIKEAADILNAQCTGTYDSDKHIKGVSIDSRNVTENSLFI
                     PIKGMTVNGHAFVKDAIKKGAAATLWNKDEPNPPHDIAVILVEDTVKGLQELSRYYRN
                     KLNTKIVGVTGSNG"
     gene            complement(505..738)
                     /gene="tlp"
                     /locus_tag="PKH42_09210"
     CDS             complement(505..738)
                     /gene="tlp"
                     /locus_tag="PKH42_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein Tlp"
                     /protein_id="HOA20653.1"
                     /translation="MDKRPKPDDRRDNVERIQFNIDNTIRNYRETKDKIKITEDEKQR
                     QELEEKNERRDQALKSMRKEIRQEATDRKNNYK"
     gene            complement(916..4482)
                     /gene="nifJ"
                     /locus_tag="PKH42_09215"
     CDS             complement(916..4482)
                     /gene="nifJ"
                     /locus_tag="PKH42_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOA20654.1"
                     /translation="MSKVMKTMDGNTAAAYISYAFTEVAAIFPITPSSPMPELIDEWA
                     AHGQKNIFGQTVRVTELESEAGAAGAVHGSLQAGALTSTYTASQGLLLMIPNMYKIAG
                     ELLPGVFHVTARAVAGHALSIFGDHSDVMAARQTGFALLASGSVQEVMDISAVAHLTA
                     IKSRVPFLNFFDGFRTSHEIQKIEVIDYEDLAKLVDMDAVDEFRKRALNPEHPVTRGT
                     AQNPDIFFQAKEASNKYYEVVPDIVNDYMKEISKLTGREYKPFVYYGAEDADRVIVAI
                     GSVTETIKEVIDYLSAKGEKVGLVAVHLYRPFSEKYFFNVLPKSVKKIAVLDRTKEPG
                     SVGEPLYLDVKSLFYNKEDAPVIVGGRYGLGSKDTTPAQIFAVYNNLIADEPKDGFTI
                     GIVDDVTFKSLEIPEVVNVSAPGTVRCKFWGLGSDGTVGANKDAIKIIGDHTDMYAQG
                     YFSYDSKKSGGVTVSHLRFGKSPIRSTYLIDEADFVSLSQQSYVDKYDVLEGLKKGGT
                     FLLNTIWSKDELDAYLPAKYKKYMAENDIKFYTINATQIAQDLGLGNRTNMIIQSAFF
                     KLADVIPIDDAVNYLYDAIESSYGKKGTTVVEMNRAAVDKGITELVKIDIPEEWKNAV
                     DDNKANSGLAVPFTEDEKPEFIKNVAEVMTRQQGDKLPVSTFAGREDGTFPHGTAAYE
                     KRGIATSVPRWIPENCIQCNQCSFICPHSVIRPTLLDENEKANAPKDFVTIKAVGKGL
                     EGLDYRIQISTLDCTGCGNCADICPGKKGNKALVMTPIAEEVEREVPNWKYAIDKVTI
                     KDDLMDKVSVKGSQFCKPYFEFSGACAGCGETAYVKTITQLFGDRMMIANATGCSSIW
                     GASAPSTPYTKDACGKGPSWANSLFEDNAEYGYGMATAVRQIRDSIQMSMEELLELDA
                     KPELKEAIQEWIANRELGEESKAASAKVLEILEGLEPCDKCAQAIKNILDKKDYLAKK
                     SQWIIGGDGWAYDIGYGGLDHVLASGEDVNVLVLDTEVYSNTGGQSSKATPTASVAKF
                     AAAGKRVKKKDLGMIATTYGYVYVAQVAIGANMNQFLKAIKEAESYQGPSLIIAYAPC
                     INHGIKAGMGKTVDREKKAVEAGYWHLYRFNPLLKEEGKNPFILDSKEPTASFREFLE
                     GEVRYASLKTSFPELAEELYAKAEKDAKDRYESYVRMAEMKY"
BASE COUNT         1218 a          968 c          735 g         1673 t
ORIGIN      
        1 ttccgttgct gcctgttacg cccactattt tagtatttaa tttatttctg taatatctgg
       61 aaagctcctg taagcctttt accgtatctt caaccaaaat aacagcaata tcatgaggag
      121 gatttggctc atctttattc cacaaggttg ccgcagctcc cttttttatg gcatctttaa
      181 caaaggcgtg cccatttacg gtcatcccct ttatgggtat aaaaagactg ttttctgtaa
      241 catttcttga atcaatactt acacctttta tatgtttgtc cgaatcatat gttcctgtac
      301 actgagcatt taaaatgtca gctgcttctt ttatattaat actaatcatc aatagttcct
      361 ttctatttaa ttacagttaa attatattgt aaaaccttga atttcgcaag gtgcaaaaac
      421 aattcctaaa aagaaaaaaa tgtcatcctg acgaagaata tatgattctt cactgtatca
      481 gaatgacaca agacatcaac atcattattt gtaattattt tttctatctg ttgcttcttg
      541 ccttatttct ttacgcatac tttttaatgc ttgatcgcgc ctttcgttct tttcttctaa
      601 ttcttgtctt tgcttttcat cttcggttat tttgatttta tcttttgttt ccctgtaatt
      661 tcttattgta ttatcaatat taaactgaat tctttcaaca ttgtctcttc tatcatcagg
      721 cttaggtctc ttatccatat tagctcctta tgttaatttt tatttttatt attgctaaaa
      781 ataaattaat taacctgcct ttttttgtta tttgataatt atagctgtgg acaaaccatc
      841 aacctgatta tattcaaacg ttaaaaagaa gacaggataa acctgtcttc ttgtagtaaa
      901 aatctcttaa tcatcttaat acttcatttc agccattctt acatagcttt catatctgtc
      961 cttagcatct ttttctgctt tagcatataa ttcttcagca agttcaggga atgaagtttt
     1021 cagtgatgca tatcttactt cgccttcaag gaattctctg aatgacgcag taggttcttt
     1081 ggaatctaat atgaacggat tcttgccttc ttctttaaga agcgggttga atctgtacaa
     1141 atgccagtaa ccagcttcaa cagctttctt ttctctgtcg acggtcttgc ccattcctgc
     1201 tttaattcca tggttgatgc atggtgcgta agcaataatc aaagacggtc cttggtagct
     1261 ttctgcttct tttattgcct ttaagaattg attcatgttt gcaccgatag caacctgggc
     1321 cacatataca tagccatatg ttgtcgcaat cattccaagg tcttttttct ttactctctt
     1381 acctgctgca gcaaattttg ccactgatgc cgtaggagtt gcttttgaag actgtccgcc
     1441 tgtatttgaa taaacctctg tatcaagtac aagtacgttc acatcttctc ctgatgccaa
     1501 cacatggtct aagccgccat atccgatgtc gtatgcccaa ccgtcaccac cgattatcca
     1561 ctgtgatttc ttagctagat agtctttttt atccaatata tttttaattg cctgagcaca
     1621 tttatcgcat ggctcaagtc cttcaagaat ttcaagaact tttgcgctcg ctgcttttga
     1681 ttcttcgcct aattctctgt ttgcaatcca ttcttgtatt gcttccttta attcaggctt
     1741 agcatccaat tccaatagtt cttccattga catttgaata ctgtctctta tttgtcttac
     1801 ggctgttgcc attccgtatc cgtattcggc attgtcttca aacaatgaat ttgcccaaga
     1861 tggtcctttg ccgcaagcat cttttgtata tggagttgat ggtgcggatg ctccccaaat
     1921 tgatgagcag cctgttgcat ttgcaatcat cattctgtcg ccgaacaatt gagttatggt
     1981 ttttacatat gcagtttctc cgcagcctgc acatgctccg gaaaactcaa agtatggttt
     2041 gcagaattga cttcccttaa ctgatacttt atccattaaa tcatctttga ttgtcacttt
     2101 atcaatagca tatttccagt ttggtacttc tctttctact tcttctgcaa ttggagtcat
     2161 taccaaagct ttattgcctt ttttacccgg gcagatatct gcacagtttc cgcaacccgt
     2221 acaatccaat gtgcttattt gaattctgta atccaaacct tctaatccct tgccaaccgc
     2281 tttaatagtt acaaaatctt tcggtgcatt tgctttttca ttttcatcaa gcaatgtagg
     2341 tcttattaca gaatgcggac atatgaatga acactgatta cactgtatac agttttcagg
     2401 aatccatctt ggaactgatg ttgcaattcc tctcttttca tatgcagctg ttccgtgagg
     2461 gaaggttccg tcttctctgc ctgcaaatgt actcactggt aatttgtctc cctgctgtct
     2521 agtcataact tcagcaacat tctttataaa ttcaggtttt tcatcttcag taaagggtac
     2581 cgccaacccg ctgtttgctt tattatcatc aactgcattt ttccattctt caggtatatc
     2641 tattttaaca agctctgtaa ttcctttatc aacagcagct ctattcattt caactactgt
     2701 tgtacctttt ttgccatagg aactttcaat agcatcatac aaataattaa ctgcatcatc
     2761 tataggaata acatctgcta atttaaagaa tgcagattgg attatcatgt ttgttctgtt
     2821 accgagtcct aaatcttgag ctatttgagt tgcattgatt gtatagaatt taatatcatt
     2881 ctcagccatg tattttttat acttggcagg caggtatgca tcaagctcat cttttgacca
     2941 aatagtgttt aataagaatg ttccgccttt tttcaatcct tcaagcacat catatttatc
     3001 tacatatgat tgctgtgaaa gcgaaacaaa atctgcttca tctattaagt aagttgatct
     3061 gatgggtgat tttccgaatc ttaagtgaga tacagtaaca ccgcctgatt tcttagagtc
     3121 atatgagaaa taaccctgtg catacatgtc agtatggtca ccgatgattt ttattgcgtc
     3181 tttgtttgca ccaactgttc cgtcagagcc aagtccccag aatttacatc ttaccgttcc
     3241 cggagctgat acatttacta cttcaggaat ttccaaagat ttaaatgtaa cgtcatcaac
     3301 aataccgatt gtgaagccgt cctttggttc gtcagctatt aagttgttat atactgcaaa
     3361 tatttgtgcc ggagttgtgt cttttgagcc aagtccatat cttccgccta ctattacggg
     3421 tgcatcttct ttgttgtaga ataatgattt aacatccaga tacaagggct caccaaccga
     3481 gccgggttct ttggttctgt ctaacactgc tattttttta acagacttag gcagaacatt
     3541 aaagaaatat ttttcagaga aaggtctgta taagtgtact gcaactaaac cgactttctc
     3601 tcctttagca ctcaaataat caataacttc tttaattgtt tctgttaccg agccgatggc
     3661 aactattaca cggtctgcat cttctgcacc gtagtaaaca aacggtttgt attctcttcc
     3721 ggtgagcttg ctgatttcct tcatataatc attaactata tcaggaacaa cttcgtaata
     3781 tttgtttgaa gcttcctttg cctggaagaa aatatccgga ttttgagcgg ttcctcttgt
     3841 aacaggatgc tcaggattta gagctctttt tctgaattca tcaacagcat ccatgtccac
     3901 taatttagct aaatcttcat aatcaattac ttcaattttt tggatttcat gggatgttct
     3961 gaatccatca aagaaattca aaaaaggtac ccttgattta atagcagtaa gatgtgctac
     4021 tgcagaaata tccattactt cctgtacgct tccagacgct aagagtgcaa aacctgtttg
     4081 tcttgcagcc ataacatctg aatggtcccc gaatattgat aaggcgtgac cagctaccgc
     4141 tcttgctgta acgtggaata ctccgggaag caattctcct gcaattttat acatgtttgg
     4201 aatcattaaa agtagtcctt gtgaagcagt gtaggttgat gttaatgctc ctgcttgcaa
     4261 tgagccgtga acagcacccg cagcgcctgc ttctgattca agctcggtta ctcttactgt
     4321 ctgtccaaaa atgtttttct gaccatgtgc cgcccattcg tcaataagct caggcattgg
     4381 tgatgaaggt gttatgggga agatggccgc gacttcggta aatgcatatg aaatatatgc
     4441 tgcagcggta tttccgtcca tagttttcat aacttttgac atttgtatcc tcctataaat
     4501 ttatttttaa ttgtaaccaa aatattgtca ttttaccaat actcttaaat tatatcttaa
     4561 ataattattt aatgcaacac taaaaaataa agtt
//
LOCUS       DAOPUE010000140         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_19365_length_4504_cov_15.536300, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000140 DAOPUE010000000
VERSION     DAOPUE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19365_length_4504_cov_15.536300"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="PKH42_09220"
     CDS             <1..1310
                     /locus_tag="PKH42_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOA20655.1"
                     /translation="RTKFAASCAEFVSRYGLDGVDLDWEYPVGGGLDSNSKSLDDKQN
                     FTLLLKEIRERLNAQELKDNKDYLLTIAAGASNYYIENTEVEKFQNYLDYVNLMTYDI
                     HGPWDAYTDFNSPLYNNNDESNQYKISIDSSVKSWLNAGLPADKLVVGIPFYGYVYTV
                     SKNTNNGLYQTHSQGRSLGFDVIKNDYLSNPEYLKSFHAESLVPWLYNGRTFISYDDS
                     HSIALKAEYIREKNLGGAMIWELSQDSEGQLLNSLYEGLYDGSILPAKDYQGHWAGDV
                     IQKWLDMGYITGYPDGTFRPENFVTRAEFVKIVNNAFGFEETAEITFTDVKEENWYYE
                     EVQKAYKEGDIIGVSKTLFAPEDYITREQAAIVLSRLLDLEGYKKGVGVFYDSSQISS
                     WAREYVGAVAYRNLIKGYEDNTFRPLDNIKRAEAVVLLDRILK"
     gene            1596..>2683
                     /locus_tag="PKH42_09225"
     CDS             1596..>2683
                     /locus_tag="PKH42_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20656.1"
                     /translation="MNKKGKDCMPSTVKRSMAIVLTVMMIISTFLSSGNLVYAVGDGE
                     MVLESASYVGVGNAIINERDVTFTVPFDYIGDTLDLSGVNYVLVTGYKLGEFDRNSSA
                     IIGGDSVTIYIPYYIGVDVENTYTTHYNIRVEREEPPKPSTFSGTISMKTTVKTDIGL
                     NNSDFTKLYTKNDGKEMGFITINPASGTIGRLKYAGSNSSYEPNTKISVSDIDSLAYE
                     PPASTIGTASYTVTAYDSDGTKVTGNAALSIKVEYPSAPDVKFSTNQDTVLKVSSTGF
                     VNASNTAVGGTFDHINFDSLPSNGSLYIDYVSSTNKGTAVVKDKSYSKAELDRMSFVP
                     NALYFGKFNISYKGYNTTGDMFTGNVEIT"
     assembly_gap    2684..2783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2784..4456
                     /locus_tag="PKH42_09230"
     CDS             <2784..4456
                     /locus_tag="PKH42_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20657.1"
                     /translation="YKGYNTTGDMFTGNVEITVIEKVVVIEKIKYSAYVNTTINFIAA
                     DFEDVFLDGTGQRLSYVKFTLPSSSRGILYSNYGLSGQSKVSSTTKYYSDDIDEISFV
                     PYTNYSGTVAIAYTGFNSRGESYTGEIEIQMIKLVTDAGDIIYKTGSYTPVDFDYDDF
                     ADECWDVTREDLDFVKFSIPSSTYGTMYYKYDTRSQAKVSSTTKYYDDDLDDITFVPN
                     PSYIGTVVITYKGYNIDDEDYTGAVKITVTKEVPVAGDIKISTKEDVAIKFDDEDFND
                     ACEDATGEELDYVTFTLPSSRNGKLYYNYTSPTKYDSEVTASRKYYYDDTPSIYRITF
                     VPYKDYYGTVTIKYTGYNIDGVSFTGSVKVEVVSMPETEGSLYFKDVTRDYSWAASYI
                     DYLAENGVVSGTGNNNYSPAQNVTRGDFMLMLYRALDLTATVRGNFSDVPKDSYYYNA
                     IAVAKSLGIAKGDGDKFMPSAGITREDAMALVDRALIIEGKRLTAGKAIDLIAFKDRN
                     SVSDYAVTSVATLVKAGIIQGNNSYLYPKSMISRAEMAVILYKVLQLD"
BASE COUNT         1505 a          721 c          911 g         1267 t
ORIGIN      
        1 caagaactaa atttgcagca agctgtgcag aatttgtttc ccgctacggg cttgatggcg
       61 ttgatttgga ctgggagtac cctgtgggag ggggacttga cagcaattca aaaagtcttg
      121 atgacaaaca aaacttcact ctgcttttaa aggaaattcg tgaaaggctg aatgctcagg
      181 agttgaagga taataaggac tatttgctca ctattgctgc aggagcaagc aattattaca
      241 tagaaaatac ggaagtggaa aagttccaaa attatttgga ttatgtaaac cttatgacct
      301 atgacataca cggaccctgg gatgcttata ctgattttaa ttcacccctg tataacaata
      361 atgatgaatc aaatcaatac aaaataagta ttgattcaag cgtcaaatca tggttaaatg
      421 caggattgcc ggcggacaag ctggtggtgg gaattccttt ttacggatat gtgtacacgg
      481 tatctaaaaa taccaataac ggcttgtatc aaactcattc tcagggcagg tcactgggat
      541 ttgatgtaat taaaaatgat tatctgagca atcctgaata cttgaaaagt tttcacgcag
      601 aatcactggt accctggctt tataacggaa gaacatttat aagctacgat gattcgcatt
      661 ccatagcatt gaaggcagaa tatataagag aaaaaaattt aggcggggca atgatttggg
      721 aattaagcca ggattcggaa ggccagcttt taaattcact gtatgaaggg ctctatgacg
      781 gctcaatcct cccggcaaag gactaccagg ggcactgggc aggagatgtt atacagaagt
      841 ggcttgatat gggatatata acaggttatc ccgatggaac cttcagacct gaaaattttg
      901 tcacaagggc tgaatttgta aaaattgtaa acaatgcttt tggttttgaa gaaactgcag
      961 aaattacatt tacagatgta aaagaagaaa actggtatta tgaagaagtt caaaaggcat
     1021 acaaggaagg ggatataata ggggtttcga aaacattgtt tgctccggag gattatatta
     1081 caagagaaca ggcagcaata gttttgtcaa gactgctaga cctggaagga tataaaaagg
     1141 gtgtcggtgt attttatgac agcagccaaa tcagcagctg ggcaagagaa tatgtaggtg
     1201 ccgttgccta tcgtaatttg attaaaggat atgaagacaa caccttcaga cctctggaca
     1261 atataaaaag agccgaggcg gttgtgctgc tggacagaat attgaagtga caaccactat
     1321 ccggctacat aattaaaaat atgtaaactg aagatttgca ctatccatgc aattcactct
     1381 tcgcttagat ggagattgat ttacataata ttaatatgta aactaaacgt ctgtatctac
     1441 tgaagatgaa acagtttttt gtgccaagtg ttgctaattt tcaaaaatta tactacaata
     1501 tagtcagttg tattttatat tctttagata aatcggagaa tgataaaatg tactgatact
     1561 tatttaaaag aaagtaaata aagagagggg taataatgaa taaaaaaggc aaagactgca
     1621 tgccatctac agttaaaaga agtatggcga tagttcttac ggtaatgatg ataatatcaa
     1681 cattcttaag ctccggtaat ttagtttatg ctgttggtga tggtgaaatg gttcttgaat
     1741 cagccagcta tgtaggtgta ggaaacgcta ttataaacga gagagatgtg acatttactg
     1801 ttcctttcga ttatatcggg gatactttag atctttcggg agttaattac gttttagtaa
     1861 ccggttataa attaggagaa ttcgatagaa attcttctgc gattataggt ggagattcag
     1921 taacaattta tataccctat tatataggtg tggatgttga aaatacatac actacacact
     1981 ataatataag agttgaacga gaagagcctc caaaaccctc aacattcagt ggaacaatat
     2041 caatgaaaac aacggtaaaa actgatattg gtttaaataa ttccgatttt acgaaacttt
     2101 acactaaaaa tgatgggaaa gagatgggtt tcattaccat aaacccggct agcggtacaa
     2161 taggcagatt aaaatatgct ggtagtaaca gcagttacga accgaatact aaaattagtg
     2221 tttcagatat tgatagttta gcttatgaac ctcctgcttc tactataggc acagcttctt
     2281 acaccgtaac agcctatgac agtgatggaa ccaaagttac cggtaatgca gcattaagca
     2341 ttaaggttga atatccttct gccccggatg ttaaattctc cactaaccag gacactgttc
     2401 ttaaagtaag ctctacagga tttgttaatg caagcaatac agctgtggga ggtacctttg
     2461 atcatataaa ttttgattca cttccttcaa atggcagcct ctatattgat tatgtgtcat
     2521 caacaaataa aggaacagca gttgttaaag ataaaagtta ctctaaagca gaattggata
     2581 gaatgtcttt tgttccaaat gccttatatt tcggtaaatt taacatatca tacaaaggct
     2641 ataacacaac aggcgatatg tttacaggca atgtagaaat tacnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnncatacaa aggctataac acaacaggcg atatgtttac
     2821 aggcaatgta gaaattactg ttatagaaaa agtagttgtt attgaaaaaa ttaagtattc
     2881 cgcatatgta aacacaacga taaatttcat tgcagctgat tttgaagatg tgtttttgga
     2941 tggaaccggc caaagactaa gttacgttaa gtttactctc ccatcatcat caaggggaat
     3001 tttgtattcc aactacggct taagcggcca atccaaagta tcatctacca cgaaatatta
     3061 cagtgatgat atagatgaaa tctcctttgt accatatacc aattattcag gaacagtggc
     3121 cattgcttat acaggcttca acagcagggg agaatcctat accggtgaaa ttgaaattca
     3181 aatgatcaag cttgttacag acgccggcga cataatatac aaaaccggct catatacgcc
     3241 tgtagatttt gattatgatg attttgctga tgaatgctgg gatgttacac gggaagattt
     3301 agattttgtc aaattttcaa taccttcatc cacttatgga actatgtatt acaaatacga
     3361 taccagaagc caggctaagg tttcttcaac aaccaagtat tatgatgatg atttggatga
     3421 tataacattt gttccaaacc cttcatatat aggaactgta gttattacat ataaaggata
     3481 taatattgat gatgaagatt acacaggtgc ggtaaaaatt acggttacca aggaagttcc
     3541 ggttgcaggt gatattaaaa tttcaacaaa ggaagatgta gcaataaagt ttgatgatga
     3601 agatttcaat gatgcatgcg aggatgccac aggtgaagaa ttggattatg taacatttac
     3661 cctgccttca tcaagaaacg gaaagcttta ttataattac acatctccca caaagtatga
     3721 ctctgaagtg actgccagca ggaaatatta ttatgatgat actccttcaa tatacagaat
     3781 aacattcgta ccttacaaag attattacgg aacagttacc ataaaatata ctggatacaa
     3841 tatagacgga gtttcattta cgggctcggt gaaggttgag gttgtatcca tgcctgaaac
     3901 agaaggctcc ctgtatttca aggatgtgac cagggattat tcatgggcgg catcttatat
     3961 agattatttg gctgaaaatg gagtagttag cggtacagga aacaacaatt acagtccagc
     4021 acaaaatgta acccgtggag atttcatgct tatgctctac agggctttgg atttaacagc
     4081 aacagtaaga ggcaatttca gtgatgttcc taaagacagc tactactaca atgcgattgc
     4141 cgttgcaaaa tcccttggaa tagcaaaggg cgacggtgac aagttcatgc cgtctgcagg
     4201 aattacaaga gaggatgcca tggctcttgt tgacagggca ttgataattg aaggcaagag
     4261 actgaccgca ggaaaagcaa tagacttaat tgcttttaag gacaggaatt cagtgtctga
     4321 ttatgcagtt acctcagtgg caaccttggt taaggcaggc ataattcaag gaaacaattc
     4381 ttacctgtat ccaaaatcaa tgatttcaag agctgaaatg gctgtaatac tttacaaggt
     4441 tcttcaatta gattaataaa ttaaaaagct gtttctcctt attggaggaa cagctttttt
     4501 ttaa
//
LOCUS       DAOPUE010000141         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_19751_length_4441_cov_9.939124, whole genome shotgun sequence.
ACCESSION   DAOPUE010000141 DAOPUE010000000
VERSION     DAOPUE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19751_length_4441_cov_9.939124"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PKH42_09235"
     CDS             complement(<1..227)
                     /locus_tag="PKH42_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20658.1"
                     /translation="MMDKIIIKGAKENNLKNISIELPRDKFIVFTGVSGSGKTSLAFD
                     TIYAEGQRRYVESLSSYARMFLGQMDKPDVES"
     gene            complement(353..2317)
                     /gene="uvrB"
                     /locus_tag="PKH42_09240"
     CDS             complement(353..2317)
                     /gene="uvrB"
                     /locus_tag="PKH42_09240"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOA20659.1"
                     /translation="MGEFKIKSEYIPTGDQPQAIEKITEGLKKNYKYQTLLGVTGSGK
                     TFTMANIIEKVQKPTLVLAHNKTLAAQLCSEFKEFFPDNAVEYFVSYYDYYQPEAYVP
                     SSDTFIEKDASINDEIDKLRHSATAALFERKDVIIVASVSCIYGLGSPIDYENLVISL
                     RPGMVKDRDEIIRKLVEIQYKRNDIAFTRGTFRVRGDTVEIFPASSSENAIRVEFFGD
                     EIDRITEINTITGEILGRRNHISIFPATHYATTEENVSRAVESIKEELQERLKQLESE
                     NKLLEMQRLEQRTNYDIEMLQEMGYCNGIENYSRHINNLKPGARPYTLLDYFPKDFLM
                     IIDESHMSIPQVRGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDEFESLINQVIFLS
                     ATPGPYEIEHSQNVVEQIIRPTGLIDPPIYVRPVENQIDDLINEIRKSTDKNQRVLIT
                     TLTKKMAEDLTSYLKSTGIKVRYLHSDVDTLERMEIIRDLRIGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTVGRAARNIDGRVIMYADRITGSMERAIKET
                     NRRREIQDKYNKLHNITPKSVIKGVRDVIEATKAAEEESKYITKGKDISISEYIIELE
                     KEMLKAAENLEFEKAAYYRDEIRELKKMSS"
     gene            complement(2586..3071)
                     /locus_tag="PKH42_09245"
     CDS             complement(2586..3071)
                     /locus_tag="PKH42_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOA20660.1"
                     /translation="MKILVMSDSHNVILNSQIELIKKEGVFDLLIHCGDKFNDAQRFA
                     EELHIKNVLKVSGNCDYSLIGQKTTIIKEIESKKFIITHGHIHNVKLNLNKLKDFAKK
                     NKADIVVYGHTHKSQNEIIDNILYFNPGSTIFPKDGRASFGIIEITEETLYSKIVPLE
                     N"
     gene            complement(3049..3666)
                     /locus_tag="PKH42_09250"
     CDS             complement(3049..3666)
                     /locus_tag="PKH42_09250"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOA20661.1"
                     /translation="MKRRIILSTGNTHKINEIKDILKDMPFEVISKNDLGYEDFDVVE
                     DGTTLEENALKKAEELHKLTKGIVIADDTGLFVDALNGEPGIYSSRYAGEHASYAENN
                     KLLLNKLKDVPKEKRTAYFKTIIAVILEDGSRLTAEGICRGYIAFQERGKRGFGYDPL
                     FVVEGTDKTFSEMSEEEKNKISHRAKALKNLKDKLEEIIEDFGDE"
     gene            complement(3984..>4441)
                     /locus_tag="PKH42_09255"
     CDS             complement(3984..>4441)
                     /locus_tag="PKH42_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOA20662.1"
                     /translation="DLVSGLAVNTRKTTGMSAALQVAQAILETGWGQSVPVDKYDGKF
                     SYNLFGIKGEGTKGSVTSNTWEEYNGVAFRIDAEFRAYNNEKESWQDHKDLLLLRERY
                     APFREVMYDSTKGAWALKRCGYATDSQYAIKLIDIIERYDLKELDEVTI"
BASE COUNT         1184 a          955 c          678 g         1624 t
ORIGIN      
        1 gattccacat caggtttatc catctgtccc aaaaacattc ttgcatagga ggacaaactt
       61 tcaacataac gtctttgtcc ttctgcatat attgtatcaa aagcgaggga agtcttgcct
      121 gaaccgctca ctcccgtaaa aactataaat ttatctcttg gaagttcaat gctgatattt
      181 ttcagattgt tttcctttgc tcccttaata attattttgt ccattattta tcctttctta
      241 ggttggggac acaccatatc gtatagtcta tgtaggtcga aataatgtcc cccaattata
      301 tgtcattctt atgctcagca cgaaagaatc tcatgaaatc cttcgcttac gctcaggatg
      361 acattttttt taattctctt atttcatctc tgtaataagc tgccttttca aactctaagt
      421 tttctgcagc ttttagcatt tccttttcta attctataat atattcagat atactgatat
      481 ccttaccctt tgtgatatat ttgctttctt cctctgccgc cttggttgct tctattacgt
      541 cgcgcacacc cttaataacg gatttcggag taatgttgtg tagcttattg tacttatcct
      601 gaatttccct tcttctgttt gtctccttta ttgctctttc catggagccg gttattctgt
      661 ctgcatacat aataaccctt ccgtcaatat ttcgagctgc ccttcccact gtctgtataa
      721 gagatgtttc cgagcgaagg aatccttcct tgtctgcatc aagtattgca accaaggata
      781 cttcaggtaa atccaatcct tcccttaaaa ggtttattcc cacaagcaca tcaaactcac
      841 cgatgcggag gtctcttatt atttccattc tttccaaggt atctacatca gaatgcaaat
      901 atcttacctt aataccggta ctttttaaat aagatgtcaa atcttcagcc attttttttg
      961 taagagttgt aatcaatacc ctttgatttt tatcggttga tttcctgatt tcatttataa
     1021 ggtcatctat ttgattttcc accggtctta cataaatggg cgggtctatc agtcccgtag
     1081 gacgaataat ctgctcaaca acattttgag agtgttctat ttcgtaggga cccggtgttg
     1141 cgcttaaaaa tataacctga ttaattaaac tttcaaactc atcaaacttc agcggtctgt
     1201 tgtcaagagc cgaaggaagt ctgaacccgt attccaccaa tgttgttttc cttgccctgt
     1261 caccgttgta catgcctctt acctgaggaa ttgacatgtg agactcatct attatcatca
     1321 aaaaatcctt gggaaaataa tcaagcaatg tataaggtct tgctccaggc ttaaggttat
     1381 ttatatgtct tgaataattt tcaattccgt tacagtagcc catttcctgc aacatttcaa
     1441 tgtcatagtt tgttctttgc tccaatcgct gcatttcgag cagcttgttt tcgctttcaa
     1501 gttgtttcaa tctttcctga agttcttcct ttatactttc aacagccctg ctcacatttt
     1561 cttcagtagt tgcatagtgg gttgcaggga atattgaaat atgatttctt ctgcctaata
     1621 tttcgcctgt tatcgtatta atttccgtaa tcctgtctat ttcatctcca aagaactcca
     1681 cccttatggc attttcagaa gacgaagcgg ggaatatctc cacggtatcc cctcttaccc
     1741 tgaatgtacc tctggtaaat gctatatcat ttcttttata ttgaatttca acaagctttc
     1801 ttattatttc atctctgtcc ttaaccatcc cgggtcttag agatataact agattttcat
     1861 aatctatagg gctacccagt ccgtaaatgc acgaaacact ggcaactatt ataacatctt
     1921 ttctttcaaa aagtgcagcg gttgcggagt gcctcaattt gtctatttca tcatttatgg
     1981 atgcgtcctt ctcaatgaat gtatcactgc taggcacata tgcctccggc tgataataat
     2041 cataataaga tacaaaatat tcaaccgcat tatcaggaaa aaattccttg aattccgagc
     2101 atagctgagc cgccaatgtt ttgttatgag ctaaaacaag ggttggcttc tgtacctttt
     2161 caattatatt tgccatagtg aaagttttcc ctgagccggt aactccaaga agtgtttgat
     2221 atttataatt tttcttcagc ccttcagtaa ttttctctat tgcttggggc tggtcaccgg
     2281 ttggaatata ttcggattta attttaaatt cacccatgaa gtgctccttt tgtctaaacg
     2341 tatgttcgct ccattatata atttttgtat tgttaagtca acaaaatctg caagcgaaaa
     2401 taagcataaa aatatgcaaa aagattgtac cagtagagga acggggacac ataaatgtcc
     2461 ccgtgttatg agattcttcg ctgcgctcag aatgacaaag cataatcatg agattcttcg
     2521 ctgcgctcag aatgacaaag cataatcatg agattcttcg ctgcgctcag aatgacactc
     2581 atgtattagt tttccaatgg tactattttg ctgtataaag tttcttcagt tatttctatt
     2641 ataccaaagc ttgcacgtcc gtcctttggg aagattgtac tgcccggatt aaaatataat
     2701 atattgtcga taatttcgtt ttgtgactta tgagtatgtc cgtatactac aatatctgcc
     2761 ttgtttttct tggcaaagtc cttcagctta tttaaattta atttcacatt atgaatatgt
     2821 ccatgagtaa taataaactt tttactttca atttctttga ttattgttgt tttctggcca
     2881 ataagactat agtcacagtt tcccgaaacc tttaaaacat ttttaatatg cagctcttcg
     2941 gcaaatcttt gtgcatcatt gaatttatcg ccgcaatgaa ttaaaaggtc gaaaacccct
     3001 tcctttttta tgagttctat ttgagaattt aatatgacat tatggctgtc actcatcacc
     3061 aaaatcttca ataatttcct ccaacttatc ttttaaattc tttagtgcct tagccctgtg
     3121 actgatttta tttttttctt cttccgacat ttccgaaaaa gttttatctg ttccttccac
     3181 aacaaataac gggtcatatc caaaacccct tttgcctctt tcctgaaatg ctatgtaacc
     3241 tctgcaaatg ccttctgcag ttaaccggct gccatcttct aaaataacag caataattgt
     3301 tttaaaataa gcggttcttt tttctttcgg aacatctttt aacttattca ggagcagttt
     3361 attgttttca gcatagcttg catgttctcc cgcatacctg gaagaatata ttccgggttc
     3421 tccgtttaaa gcatctacaa ataatcccgt atcatcggct atgactatcc cctttgtgag
     3481 tttgtgaagc tcttcagcct ttttaagagc attttcctct aatgtggtgc catcctccac
     3541 tacatcgaaa tcttcatatc ccaaatcatt tttagatatc acttcaaagg gcatatcctt
     3601 taaaatatct tttatttcat taattttatg tgtattaccg gttgataaaa ttattcttct
     3661 cttcattcat ccttccccgc ttaatttctt tagtttgtcg taccctatat ttctctgtga
     3721 cttccctaag tatcttaaaa tatcgtcatt gctatgaata agctacgtgt cattctgaga
     3781 gctttagctc gaagaatctc atttttacca tgagattctt tgcttactct caggatgata
     3841 tcttatattt attatatcct aattggattt atttaacaat aatattgcaa gccgaaggaa
     3901 ggggacaaac cttcaaaata agttaactca aaataataat ttttgaagga tgtccccaat
     3961 attttactgc tatcagaata actctatatt gttacttcat ccagctcttt taaatcatat
     4021 ctctcaataa tgtctatcag tttgattgca tactgtgaat ctgttgcata tccgcatctt
     4081 tttagcgccc aagctccttt tgtgctatca tacataactt ctctgaaagg tgcatacctt
     4141 tctcttaaca gcaataagtc cttgtggtcc tgccagcttt ctttttcatt gttgtatgct
     4201 ctgaactcag catctattct gaatgcaacg ccgttatatt cttcccaggt attagaggtt
     4261 acggagccct ttgtcccttc acctttaatt ccaaaaagat tgtatgaaaa tttaccatca
     4321 tatttatcaa cgggtacact ttgtccccag cctgtttcca atatagcctg tgcaacctgc
     4381 aatgcggcgg acatgcctgt ggtttttctt gtatttacgg ctaaaccgga aaccaagtcc
     4441 a
//
LOCUS       DAOPUE010000142         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_19888_length_4420_cov_9.600458, whole genome shotgun sequence.
ACCESSION   DAOPUE010000142 DAOPUE010000000
VERSION     DAOPUE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19888_length_4420_cov_9.600458"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PKH42_09260"
     CDS             <1..359
                     /locus_tag="PKH42_09260"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOA20663.1"
                     /translation="SIRCNNCNKEANIDDFIKNHKCEFCGSNILRPNVVLFGESLPAG
                     PWNQAQSDIRNSDLLIVIGTSLEVSPVNQLPLLTRGKRALINNEDVYTYYDFDIKLIG
                     KAKDILIRLRDTYNKI"
     gene            complement(406..1311)
                     /locus_tag="PKH42_09265"
     CDS             complement(406..1311)
                     /locus_tag="PKH42_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOA20664.1"
                     /translation="MREFIQTHTGEIAALATAVCFTLNAISFESAGKKVGSLSVSYIR
                     LFIAFILLSISAYITRGLIMPTDATTSAWFWLLISGLFGFVLGDLFLFEAFVEIGSRI
                     SLLIMSASPPITALAGFLFMREKLSIFALIGMTLNMLGISLVILSRGADEKKVEFNRP
                     VKGIIFACLGALGQAAGLIFSKIGMGSYNPLAATQIRIIAAVIGYTIIIAWRSKWQEI
                     KDSFKNKKAILQIAAGSVTGPYIGVVLSLVALQHTAAGIVSSITSISPIIIIPASIVI
                     FKEKILPKEILGAFISIAGVMLLFL"
     gene            complement(1374..2171)
                     /locus_tag="PKH42_09270"
     CDS             complement(1374..2171)
                     /locus_tag="PKH42_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOA20665.1"
                     /translation="MMLGMPALVEYTSLDQLIELCMKLKLDFIELNMNLPYNFKQNLP
                     PNELKKITKDTNIKFTMHMPDEADIGSFYESVREGYVQLFSDTIEWAKEAGVKLLNMH
                     IIEGPKMTLPDTKVYIYDKYSEEFTDNFIKSIKTLSNKAEKNNIILAIENSNNFSKKY
                     IQAALDKALTYPKIKLTWDTGHNAVSNFKDEIYLMQNREHIAHMHLHDAKGMNDHQVL
                     FEGELNIKKLLNFARSMNIMTLIEVKTEEALIKSVNNLSKNYSRGEL"
     gene            complement(2369..3100)
                     /locus_tag="PKH42_09275"
     CDS             complement(2369..3100)
                     /locus_tag="PKH42_09275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOA20666.1"
                     /translation="MKLRIDKMLSNSGIGSRKQIKTDARKGFVEVNGTIEKDSSKIID
                     TETDTVKYKGEVIKYTQYIYLMMNKPRGVVSATEDNYDKTVIDLLDDEEKFYEPFPVG
                     RLDKDSEGLLLITNDGKLAHELLSPRKHVDKTYYVEVAEEITEDDVRAFEEGVILKED
                     NYKTLPAKLQIMESGYPSICLVTIREGKFHQLKRMFNAVNNEVTYLKRISMGALKLDE
                     SLKTGQYRHLTEEEINLLRQENKLT"
     gene            complement(3200..>4420)
                     /gene="murA"
                     /locus_tag="PKH42_09280"
     CDS             complement(3200..>4420)
                     /gene="murA"
                     /locus_tag="PKH42_09280"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOA20667.1"
                     /translation="LKGNIRVDGSKNSILPILAATLLCKEECIIHDIPDLQDVKIMCK
                     LLEELGAKVEKEGMNSLKITAENIKTCEAPYELISKMRASFLVMGPLLTRCHNAKVCM
                     PGGCAIGTRPIDLHLKGFKYLGSEVKSESGYIHATAEKLIGRDIYLDFPSVGATENII
                     MAATLAEGETILENAAEEPEIVDLANFINSMGGNIIGAGTKTVRIKGVEKLHKTEHTV
                     IPDRIEAGTYMVAAAATGGDVMIENVVPSHLQPVIAKLREAGATVEEYDDKIRVMARD
                     TIKSLDIKTLPYPGFPTDMQAQFMAMLSKADGTSIINETIFENRFMHVNELSRMGLNV
                     KVEGSSAVLKGSSPLSGAKVRATDLRAGAALIIAGLLAEGETEITEVYHIRRGYANIV
                     EKLRGLGADIKYEE"
BASE COUNT         1220 a          885 c          715 g         1600 t
ORIGIN      
        1 ggtctataag gtgcaataat tgtaataagg aagcaaatat agatgacttt attaagaatc
       61 ataaatgtga attttgcggc agcaatattt taagacctaa tgtcgtattg tttggagaat
      121 cattgcctgc aggcccttgg aatcaagccc aatcagatat tagaaattca gatttgctga
      181 tagtcatagg aacaagctta gaagtatctc cggtaaatca acttccgctt cttacaagag
      241 gaaagcgtgc gctaattaat aatgaagatg tatatactta ttatgatttt gacattaagc
      301 ttattggaaa ggctaaagat atattaataa gactaaggga tacttacaat aaaatatgaa
      361 atcggtgtca ggcaccgtat gtgtgtgcct ggcaccccta tttggttaca agaataacaa
      421 cattacccct gctatggaaa tgaatgctcc caatatttct ttcggtagaa ttttctcttt
      481 aaaaataaca attgaagcag ggattataat tataggcgat atggatgtaa tggatgaaac
      541 tattcctgca gcagtatgct gcagtgccac cagcgaaaga acaacaccaa tataaggtcc
      601 tgttacagag cctgctgcta tttgcaatat tgctttttta tttttgaaag agtcttttat
      661 ttcttgccat ttgcttctcc aggctattat tattgtataa ccgattacag cggcaattat
      721 tcttatttgc gtagccgcca aaggattata tgaacccatc cctattttgc tgaatattaa
      781 acctgcagct tgtccaagag cacccaaaca agcaaatata attcctttca cagggcgatt
      841 gaattcaacc tttttttcat cagctcccct gctaagtata acaagactaa taccaagcat
      901 gtttaaagtc attcctatca aggcaaaaat tgataatttt tccctcataa acagaaatcc
      961 cgccagtgcc gtaattggcg gagatgctga cattataagc aatgatattc ttgagcctat
     1021 ttctacaaat gcttcaaata aaaataaatc ccctagtaca aatccaaaca atccggatat
     1081 caatagccaa aaccatgcgc ttgttgttgc gtcggtgggc ataataagtc ctcttgtaat
     1141 ataagcactg atgctcagca aaatgaaagc aatgaataac cttatgtagc ttacagataa
     1201 ggagccaact ttcttacctg ccgattcaaa agaaattgca ttcaatgtga agcatactgc
     1261 tgtagccaat gcggctattt ctcctgtgtg tgtttgtata aattctctca tagtcattcc
     1321 cttttttcta atttaattga tgtcttaatt taattacata ccttagtaat tggttagagc
     1381 tcacctctac tatagttctt gcttaaatta tttactgatt ttattagtgc ttcttccgtc
     1441 ttcacttcta tcagtgtcat aatattcatt gaccttgcaa agtttagcaa tttcttaata
     1501 ttcagctcgc cctcaaatag aacttgatgg tcgttcattc cttttgcatc atgaagatgc
     1561 atgtgagcaa tgtgctcccg gttttgcatc aaataaattt catccttaaa attgcttact
     1621 gcattatggc ctgtatccca agtaagtttt atttttgggt atgtcaaagc tttatccaag
     1681 gcagcttgta tgtatttttt cgagaagtta ttgctgtttt ctattgctaa tattatgttg
     1741 tttttctctg ctttatttga aagggttttt attgatttaa tgaagttatc tgtgaattct
     1801 tctgaatatt tatcataaat ataaactttt gtgtcaggaa gggtcatttt tggcccttca
     1861 attatgtgca tgttaagaag ctttactcct gcttcttttg cccattcaat ggtatccgaa
     1921 aacagttgta catatccttc tctgacactt tcataaaaag agcctatatc tgcttcatcg
     1981 ggcatgtgca tagtaaattt aatattagta tcctttgtaa ttttcttaag ttcattcggg
     2041 ggtaaatttt gtttgaagtt atagggtaga ttcatgttta attctatgaa atcaagcttt
     2101 agtttcatgc atagttcgat tagttggtcc agtgatgtat attcaactaa agcaggcatg
     2161 cctaacatca taatgacatt tctcctttta taagtatatg agattcttcg agctagctct
     2221 atgaattatg aagtgtttac tacaccacga taaataaata cgctttgtca tcctgagcgt
     2281 aagcgaagga tctcatgaga ttcttcgagc taaagctctc agaatgacac gtagctaatt
     2341 catagcaata aaaaaatgtt attcttaact atgtcagttt attttcttgt cgcaataaat
     2401 ttatttcttc ctccgttaag tggcggtatt gtcctgtttt taaagactcg tcgagcttta
     2461 atgctcccat ggatattctt tttaaatatg ttacctcatt gtttacggca ttgaacattc
     2521 ttttaagctg gtggaacttt ccttctctta ttgtaacaag gcatattgaa ggatatccgg
     2581 attccattat ttgaagtttt gcaggtagtg ttttataatt atcttcttta aggattactc
     2641 cttcttcaaa ggctcttaca tcatcttcag ttatttcttc agcaacttcc acgtaatatg
     2701 ttttgtccac atgttttctc ggtgacaata attcatgtgc taactttccg tcatttgtta
     2761 taagaagcaa accttctgaa tccttgtcaa gccttccaac cggaaatggc tcgtaaaatt
     2821 tttcttcatc atctaataaa tctataaccg tcttgtcata attatcttct gttgctgaaa
     2881 ctactcccct tggtttattc atcatcaggt aaatatactg cgtatatttt attacttcgc
     2941 ctttatattt tactgtatcg gtttcggtgt ctattatttt tgagctgtct ttttcgattg
     3001 ttccgtttac ttcaacaaat ccttttcttg catccgtctt tatctgcttt cttgagccga
     3061 tgcctgaatt agacaacatc ttatctattc gaagtttcat ttttccctct attgcttttt
     3121 acagtattat aacatgatga atataagtgt caataaatat aaaatagtgc caggcaccgt
     3181 ctccggtgcc aggcaccaat cattcctcat atttaatatc cgctcctaat ccccggagct
     3241 tttccactat atttgcatag cctctcctaa tatgatagac ttcagtaatt tcagtttctc
     3301 cctcagccaa caatcctgca ataataagag ctgcacctgc ccttaaatcc gttgctctta
     3361 cttttgcacc gcttaagggg ctgcttcctt ttaaaacagc gctgcttccc tcaaccttaa
     3421 catttaatcc cattctagac aattcattta catgcataaa tcggttttca aaaatagttt
     3481 catttattat gcttgtcccg tccgcttttg aaagcattgc catgaactga gcctgcatat
     3541 ccgttggaaa acccggatat ggaagtgttt ttatatccaa ggattttatt gtatcccttg
     3601 ccataaccct tattttatcg tcatactctt ctactgtagc tcctgcttct cttaattttg
     3661 ctattacagg ctgcaaatga ctcggaacta cattttcaat cattacatct ccgcctgttg
     3721 cagctgcagc taccatgtaa gtacctgctt caattctgtc aggtattacg gtatgctctg
     3781 ttttatgaag cttttcaaca ccttttattc taactgtctt ggtgcctgct cctataatat
     3841 tgcctcccat tgaatttata aaattggcta agtcaactat ttcaggttct tctgccgcgt
     3901 tttccaagat tgtttcacct tctgcaaggg ttgctgccat aattatgttt tctgttgctc
     3961 caacactggg aaaatcgaga tagatatctc ttccaattaa tttttctgct gttgcatgga
     4021 tgtatccgct ttctgattta acttcggagc cgaggtattt aaatcctttt aaatgcaaat
     4081 caataggcct tgtccctatg gcacagccac cgggcataca tacctttgca ttgtgacacc
     4141 ttgttaataa ggggcccatt accaaaaagg atgctctcat tttgcttatg agctcatatg
     4201 gtgcttcgca ggttttaatg ttctcagcag ttatttttaa tgaattcatc ccttcttttt
     4261 caacctttgc acctaattct tccagaagtt tgcacattat tttaacatcc tgcaaatcag
     4321 gaatgtcgtg aattatgcat tcttccttac ataaaagcgt tgctgccaat atgggcagta
     4381 tagaattttt tgagccgtca actcttatgt ttcctttaag
//
LOCUS       DAOPUE010000143         4326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_20460_length_4326_cov_10.990635, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000143 DAOPUE010000000
VERSION     DAOPUE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20460_length_4326_cov_10.990635"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PKH42_09285"
     CDS             complement(<1..362)
                     /locus_tag="PKH42_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20668.1"
                     /translation="MDSWILIEAFVNFLQNYIIYKATGLFYDRRLSFKFSVEIVIVIM
                     TLILTGLNYYSQIHSNPLLYFAYCLLLLVTAAIIYKGNIISKAVIIFLLIVVTGILEL
                     LAAVLITTLGGFDINTIQ"
     gene            complement(378..1085)
                     /locus_tag="PKH42_09290"
     CDS             complement(378..1085)
                     /locus_tag="PKH42_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOA20669.1"
                     /translation="MVNIAVCDDSKEYVEIVRELINVIMVKNNTACNIYTYISGLSLV
                     QDFRVKTFDIIFLDMEMPEIDGIKTGLLIREISDKVIIFYLTSHKEYAYESYQVKAKN
                     YLLKPIQSDVLEKELMECIAELDMPLEFLDVKDTDGIFHRIPLCDITHILRKKEDRKI
                     HIYTLDKQEIIAVQTLKNIENSLSSISFFKRSCKSCLVNLNNIRAIHKNIIVFINGET
                     EEASRRCLSDLVNLFKK"
     gene            complement(1354..1695)
                     /gene="rplQ"
                     /locus_tag="PKH42_09295"
     CDS             complement(1354..1695)
                     /gene="rplQ"
                     /locus_tag="PKH42_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOA20670.1"
                     /translation="MGSHRKLGVKSDHRVAMLRNMTADLITYGRITTTVTRAKELRRV
                     AERIITLGKRGDLHARRQALAYLYDKNAVYKLFEEVAPKYKDRNGGYTRILKLGPRRG
                     DGAEMAIIELV"
     gene            complement(1714..2661)
                     /locus_tag="PKH42_09300"
     CDS             complement(1714..2661)
                     /locus_tag="PKH42_09300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOA20671.1"
                     /translation="MIEIEKPNITLNEKNENNTYGKIVVEPLERGYGTTIGNSLRRIL
                     LSSLPGAAVTSINIQGVLHEFSTIPGVREDVTEIILNIKNLAAKMNVPGPVQLLIDAK
                     GPKEVTAADIITGGDVDIINEDLHIATLEEGSELIIEMEMEQGRGYAIAERNKKDSQA
                     IGVIPIDSIYTPVKKVNFTVEDTRVGNRTDFDKLTLEVWTNGTIEPEEAVSLGAKILN
                     EHLNLFISLTEHVNEVEIMVEKEEDEKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LTYKTEEDMMKVRNLGKKSLDEVSKKLEELGLSLRKNDN"
     gene            complement(2713..3336)
                     /gene="rpsD"
                     /locus_tag="PKH42_09305"
     CDS             complement(2713..3336)
                     /gene="rpsD"
                     /locus_tag="PKH42_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOA20672.1"
                     /translation="MARYTGPVCRICRREGLKLYLKGDRCYTDKCSIGRRNYAPGQHG
                     QNNKKLTNYGTQLREKQKVRRYYGVLEGQFKEYYNIAEKMSGKTGDNLLKLLELRFDN
                     VIYRLGFANSRPEARQLVVHGHFTVNGKKADIPSMILQVGDEIKVKEKSQNSDKFKIM
                     AENHKNTAPKWLQVEPENFTGRIIAEPTKEDIEIPIEEHMIVEFYSK"
     gene            complement(3355..3753)
                     /gene="rpsK"
                     /locus_tag="PKH42_09310"
     CDS             complement(3355..3753)
                     /gene="rpsK"
                     /locus_tag="PKH42_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOA20673.1"
                     /translation="MAAKKQKTTRVRRRERKNIEKGQAHIKSTFNNTLVTLTDLQGNA
                     ISWASSGQLGFKGSRKSTPYAASMVAEEAAKKAMEHGLKTVEVYVKGPGSGREAAIRS
                     LQAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(3769..4137)
                     /gene="rpsM"
                     /locus_tag="PKH42_09315"
     CDS             complement(3769..4137)
                     /gene="rpsM"
                     /locus_tag="PKH42_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOA20674.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRPTSIKILKATGINPDTR
                     VKDLTEDEIQKLRTEIDKHPVEGDARREVALNIKRLKEINCYRGLRHKKGLPVRGQNT
                     KNNARTRKGPKRVSGKKPKK"
     gene            complement(4184..4297)
                     /gene="rpmJ"
                     /locus_tag="PKH42_09320"
     CDS             complement(4184..4297)
                     /gene="rpmJ"
                     /locus_tag="PKH42_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOA20675.1"
                     /translation="MKVRPSVKPLCEKCKIIRRKGKVMVICENPRHKQKQG"
BASE COUNT         1124 a          911 c          611 g         1680 t
ORIGIN      
        1 tcttgaatgg tgtttatatc aaaacctcct aatgtggtaa tcaacactgc tgccagcaat
       61 tcaaggattc ccgtaactac tataagcaaa aatattatta cagcctttga aataatattg
      121 cctttatata ttattgctgc tgttacgagt aataataaac agtaagcaaa atataataga
      181 ggatttgaat gtatttggga ataataattc aatcctgtaa gtattaaggt cattataact
      241 attacaattt caacgctgaa tttaaacgat aaccttctgt cgtaaaacag tcccgtggcc
      301 ttataaataa tataattttg aagaaaatta acaaaagcct caattaaaat ccatgaatcc
      361 actctatcac ctcttgttta ttttttaaat aaattcacta aatccgaaag gcatcgtctg
      421 ctggcttctt ctgtttcacc gtttataaaa acaataatat ttttatgtat tgctcgtata
      481 ttattaagat ttacaagaca ggatttacag gatcttttga aaaaactgat tgaagacaga
      541 ctgttttcta tgtttttaag agtttgaacc gctataattt cttgtttatc caaagtataa
      601 atatgtatct ttctatcttc tttttttcta agtatatgag ttatatcgca taaaggaatg
      661 cggtgaaata ttccgtctgt gtcttttaca tcaagaaatt caagaggcat atccaattct
      721 gcaatgcact ccatcagttc tttctccaat acatcgcttt gaataggctt aagtaaataa
      781 tttttagcct ttacctgata tgattcatat gcatattctt tatgagatgt caggtaaaaa
      841 ataataacct tgtcgcttat ttcccttatt aaaaggcctg tttttatacc atcaatttcg
      901 ggcatttcca tatccagaaa aattatgtca aacgttttca ctctaaaatc ttgtacaagg
      961 gataagcctg aaatgtatgt atatatatta caggctgtat tatttttcac cattattaca
     1021 tttattaatt ccctgactat ttcaacatac tctttagaat catcacaaac agcaatatta
     1081 accatatttt tccattgtta ctccgtgtaa ttgaaatgta attgctagtt aataatatca
     1141 taataatacg attatttcaa tgtttattta tacattcgtc aaaaatccat aaaatttaag
     1201 aacggctcat tttgcccgta agaacaaaag aaccgctcct ctacaattgt cattctgaga
     1261 ggtctgcccg aaagattctc atgagatcct tcgcttacgc tcaggatgac attgtcttta
     1321 gatccttcga ttttactcag gatgacaact atattatact aattcgatta tagccatttc
     1381 tgctccatcg cctcttctgg gccccaattt tagaattctg gtgtatccac cgtttctatc
     1441 tttgtattta ggagcaactt cttcgaatag cttatatact gcatttttat cgtataaata
     1501 agctaatgcc tgacgtcttg catgcaggtc tcctcttttt ccaagagtga taattctttc
     1561 tgcgactctt cttaactctt tggctcttgt aaccgtagtt gttattctgc cgtaagttat
     1621 aaggtcagca gtcatatttc gaagcattgc cactctgtgg tcagatttaa ccccaagctt
     1681 cctgtgactt cccatttagt cctccttttt aagctaatta tcgtttttcc ttaaagataa
     1741 tcctaattcc tcaagtttct tcgatacctc atccaaagat tttttcccga ggtttcttac
     1801 cttcatcatg tcttcctcag tcttgtaagt aagctcctca acagtgttta ttcctgctct
     1861 cttcagacaa ttgtatgacc ttaccgataa atcaagttcc tctattgtca tttccaaaac
     1921 tttttctttt tcatcttctt ctttttcaac cataatttca acttcattaa catgttctgt
     1981 caatgaaatg aataggttga gatgctcatt caatattttt gcacctaatg aaactgcttc
     2041 ttcaggctct atcgtaccat tagtccagac ctcaagagta agtttgtcga aatccgttct
     2101 gtttcccact cttgtgtctt caaccgtaaa gtttactttt tttacaggcg tataaattga
     2161 atcaatagga ataactccga ttgcctggct atccttcttg tttctttctg ctatagcata
     2221 tcctcttccc tgctccattt ccatttcaat aatgagctcg gagccttctt caagtgttgc
     2281 tatatgcagg tcttcattaa taatgtccac atctccgcct gtaattatgt ctgccgccgt
     2341 aacctctttc ggcccttttg cgtctatcaa tagctgcaca ggtccaggta cattcatttt
     2401 agctgctaaa ttcttaatat taagaattat ttctgtcaca tcttctctca ctccgggtat
     2461 tgtagaaaat tcatgcaaca ctccttgaat gtttattgaa gtaactgctg ctcccggtaa
     2521 agatgaaaga agtattctcc ttaatgaatt tccgattgtt gtcccgtagc ctctttccag
     2581 gggctccacc actattttac cgtaggtgtt attctcattc ttttcattta aagtaatatt
     2641 tggtttttct atttctatca tcccaaccct ccttaatttt ttgtttgtcg agggtaatct
     2701 tcttaaacgc tattacttgg agtagaactc gacaatcata tgctcttcta ttggtatttc
     2761 aatatcttct ttagttggct cagcaattat tcttcctgtg aaattttcag gttcaacctg
     2821 caaccattta ggtgcagtgt ttttgtgatt ttctgccata attttaaatt tgtctgaatt
     2881 ttggctcttt tcttttactt ttatctcgtc accgacttgt aatatcattg acggtatatc
     2941 agcctttttg ccgtttacgg taaaatgacc gtgaactacc aattgtcttg cttcaggtct
     3001 tgaatttgca aagcctaatc tgtaaattac attgtcaaat ctaagctcta acaatttcag
     3061 caaattatca cctgtttttc cggacatttt ttctgcaata ttgtagtatt ctttaaattg
     3121 tccttccaaa actccgtaat atcttctaac cttttgtttt tctcttaact gagtaccata
     3181 gttagtcagt ttcttgttat tttgaccatg ctgaccggga gcataatttc ttcttccgat
     3241 tgagcatttg tcggtataac atctatcacc ttttaaatat aactttaaac cttctcttct
     3301 gcatattctg caaacaggcc cggtatatct tgccatcttt tcacctcgtt tctattatac
     3361 tctcctgcgc tttggcggtc tgcaaccatt gtgcggtatt ggagtaacgt cttttattaa
     3421 atttacttca agtccggctg cctgcagtga acgaatcgct gcttctcttc ctgagcccgg
     3481 tcctttaaca tatacctcta ctgttttcaa tccatgctcc atagcttttt ttgctgcttc
     3541 ttcagcaacc atgcttgccg catatggtgt agattttctt gaacccttaa atcctaattg
     3601 accggaactt gcccatgaaa ttgcatttcc ctgcaagtcg gtaagagtaa caagtgtatt
     3661 gttaaaggta gacttaatat gagcctggcc tttttctata tttttacgtt ctcttcttct
     3721 tactcgagtt gttttttgtt ttttagctgc cacttaagtc cctccttttt attttttagg
     3781 ttttttacct gaaactctct taggaccttt tcttgttctt gcattatttt tagtattttg
     3841 tcctcttaca ggcaatcctt ttttgtgtct taatcctcgg tagcagttga tttctttaag
     3901 tcttttaata ttcaacgcta cttctcgtct cgcatctcct tctacgggat gcttatctat
     3961 ttcagttctt aacttttgaa tttcatcttc cgtcaaatcc ttaacccttg tatcagggtt
     4021 gatacctgtt gcttttaaaa ttttgataga agttggtctg cctattccgt aaatatatgt
     4081 taagccaatt tcaactctct tatctcttgg caaatcaaca cctgcgattc tggccattaa
     4141 gctacacctc ctacattcgt agttttaata tttaatttat ccattaacct tgtttttgtt
     4201 tgtgccttgg attttcgcaa attaccataa cctttccttt tcgtctgata atcttgcact
     4261 tttcgcacaa aggttttact gatggtctta ctttcattgt aatacctcct tactttttcc
     4321 tccatg
//
LOCUS       DAOPUE010000144         4305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_20586_length_4305_cov_8.875765, whole genome shotgun sequence.
ACCESSION   DAOPUE010000144 DAOPUE010000000
VERSION     DAOPUE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20586_length_4305_cov_8.875765"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PKH42_09325"
     CDS             complement(<1..1148)
                     /locus_tag="PKH42_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOA20676.1"
                     /translation="MINTLVIGLKGFLATSGFSAITPGQVIMLIVSFVLLYLAIVKQF
                     EPLLLLPIGFGMFLANLPLAGLMAEPVGKFGQEGFQAGGLLYYLYMGVKLGVYPPLIF
                     MGVGAMTDFGPLIANPKSILLGAAAQLGIFFVFVGAILLGFNPQEASSIGIIGGADGP
                     TAIYLTSKLAPHLLGPIAIAAYSYMALVPIIQPPIMRALTTKEERCVVMEQLRPVSKK
                     ERVIFPIFVTLLVSLILPSAAPLVGMLMMGNLLRESGVTERLSKTAQNELMNIVTIFL
                     GTTVGATANAETFLRPQTLMIIGLGLLAFGFGTAGGVLLGKVMYKATGGKVNPLIGSA
                     GVSAVPMAARVSQVVGQQENPGNFLLMHAMGPNVAGVIGSAVAAGLLLS"
     gene            complement(1230..1640)
                     /locus_tag="PKH42_09330"
     CDS             complement(1230..1640)
                     /locus_tag="PKH42_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOA20677.1"
                     /translation="MKKYLITVNGVTYEVMAEEVRGNQPVIQQIIQQPAPVVQPAPAP
                     APTPAPAPAPVVKEAPKAAPTGGKQITSPMPGSIFKVNVKPGDSVKRGDVVIILEAMK
                     MENEIFAADDAVVASVEVKEGATVNTGDVLVIFE"
     gene            complement(1658..2092)
                     /locus_tag="PKH42_09335"
     CDS             complement(1658..2092)
                     /locus_tag="PKH42_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOA20678.1"
                     /translation="MLFSETMSMGERFISSMAITILGMSVVFAVLVIIAYSLDFLRVL
                     FGDKDKKKVAANEEGLISKEESIDTTQNKSEDEVDPNLLAVLTAAIAVQNTDELLAVL
                     TAAVLAQSGSTEEIIIKSIMPTKQKRSIWASAGRQEQMLKTI"
     gene            complement(2126..3670)
                     /locus_tag="PKH42_09340"
     CDS             complement(2126..3670)
                     /locus_tag="PKH42_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOA20679.1"
                     /translation="MSNKTEEVYSRKSKLKQGGGPKRIEKQHAQGKLTARERLSLLFD
                     EGSFREIGLFVKHRCTDFGMEEVETPGEGVVTGYGTVDGRLVYAYAQDFTVLGGSLGA
                     MHAKKITTVMDLALKMGAPIIGMNDSGGARIQEGVDALSGYGSIFYRNTISSGVIPQI
                     SAIMGPCAGGAVYSPAITDFTFMVDKTSQMFITGPQVIKAVTGEEISPEALGGAMAHN
                     AISGVAHFVSANDEACIQEIKRLLSFLPSNNLETSPLVETNDDLNRVEEKLNDIVPEN
                     PNKAYDVKEIIQTIADNNDFLETQPYYAKNIVTGFIRINGQTIGVIANQPRHLAGCLD
                     IDASDKSSRFIRTCDCFNIPLLNLVDVPGFLPGSAQEHGGIIRHGAKMLFAYSEATVP
                     KVTLILRKAYGGAYLGMCSKELGADVVLAWPSAEIAVMGPDGAANIIFKREIENADNP
                     EEERKAKIEEYKEKFSNPYVAAARGFVDDVIEPSITRPILAGAFEMLSSKRETRPAKK
                     HGNLPV"
     gene            complement(3890..4291)
                     /gene="mce"
                     /locus_tag="PKH42_09345"
     CDS             complement(3890..4291)
                     /gene="mce"
                     /locus_tag="PKH42_09345"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOA20680.1"
                     /translation="MVKKVDHIGIAVKNLDEALNFYENILGLKSAGTETVEEQKVRVA
                     FLPLGDTEVELLESTEEDGPIARYIAKNGEGIQHIAYRVDDIEKAIAEMKENGIRMID
                     EKPRYGAGGAKIAFCHPKSTFGVLIELCQRD"
BASE COUNT         1067 a          984 c          679 g         1575 t
ORIGIN      
        1 gaaagcaata atcctgcagc aactgctgaa cctattacgc ctgccacatt aggtcccatg
       61 gcatgcatta ataaaaagtt ccccggattt tcttgctgtc ctacaacttg tgatacacga
      121 gctgccatcg gtaccgcaga tactcctgct gacccgataa gaggattaac ttttcctcct
      181 gttgccttat acataacttt tcctaacaat actcctcctg ctgttccaaa gccgaaagct
      241 aataatccta atcctattat cattaaagtt tgaggtctta agaatgtttc tgcatttgct
      301 gttgctccaa cagttgttcc caagaatatt gtcactatgt tcatcagctc attctgtgct
      361 gtttttgata atctttccgt tactcctgat tctctcaata agttacccat catgagcatt
      421 ccaaccaatg gtgctgctga cggaagtatt aatgatacaa gtaatgttac aaatataggg
      481 aatattactc tttccttttt agataccggc ctaagctgct ccattacgac acatctttct
      541 tccttcgtcg taagtgccct cataatcggc ggctggatta tcggaacgag tgccatatat
      601 gagtatgccg ctatagcaat tggtcctaat aaatgcggtg ccagctttga tgtcaggtat
      661 atagccgtag gaccgtctgc tcctcctata attcctattg aagatgcttc ttgtggatta
      721 aagcctaata gtattgctcc cacaaacaca aagaatatac ctaattgtgc tgccgctccc
      781 aataatatac tctttggatt tgctattaac ggaccaaagt ctgtcattgc tcctacaccc
      841 ataaatatga gaggcggata tacgcctaat tttactccca tgtatagata ataaagcagt
      901 cctcctgctt gaaatccttc ttgcccgaat ttccccaccg gttctgccat caatcctgct
      961 aatggaaggt tagctaaaaa cataccgaat cctattggta gtaataataa gggctcaaat
     1021 tgttttacaa ttgctaaata tagaagtaca aaagatacta ttagcatgat aacttgtccc
     1081 ggtgttattg ctgaaaatcc tgaagttgct aaaaaaccct ttagtcctat taccaacgtg
     1141 ttaatcattt atgaatacct catttctcat taatatctaa atgattaagg tgtcaaaagt
     1201 aattgcgttt cttttgacac tatattcatt tattcaaata tcaccaatac atctcctgtg
     1261 tttacggttg ctccttcttt tacttctact gatgcaacta ctgcatcatc tgctgcgaat
     1321 atttcatttt ccattttcat tgcttctaat attattacta catctcctct ttttactgaa
     1381 tctccgggtt taacattaac tttgaatatg cttcccggca ttggtgatgt tatttgtttt
     1441 cctcctgttg gtgctgcctt tggcgcttcc ttcacaacag gtgcaggtgc tggtgctgga
     1501 gttggagctg gtgcaggtgc cggctgcaca accggtgcag gctgctgaat tatttgttgt
     1561 attactggtt gatttcctct tacttcttcc gccattactt cgtatgttac gccattaact
     1621 gttattaaat attttttcat gatttaatct ccttttgcta tattgttttt aacatttgtt
     1681 cttgtcttcc tgccgaagcc catatacttc tcttttgctt agtcggcata attgatttaa
     1741 ttattatttc ttctgtgctt ccgctttgag ctaagactgc tgctgtcagc actgctaaca
     1801 attcatctgt attttgaact gcaattgctg ctgtcaatac tgcaagtaaa tttgggtcaa
     1861 cttcatcttc tgatttgttt tgagtcgtat caatactttc ttctttactg attaaccctt
     1921 cttcattagc agcaactttt tttttatctt tatctccaaa taaaactctt aaaaaatcaa
     1981 gggaataggc tataataacg agtaccgcaa acactactga cattccaagt attgttatcg
     2041 ccatactact tataaatctc tcacccattg acattgtttc gctgaatagc attgaatcac
     2101 ctcttttatt tatagttgta tagcattata ccggaagatt tccatgtttc ttggccggtc
     2161 ttgtttccct cttgctagat agcatttcaa atgcacctgc aagtattggt cttgttattg
     2221 acggctcaat tacatcatct acaaatcctc ttgcagcagc tacatatggg tttgagaatt
     2281 tttctttata ttcttcaatt tttgcttttc tttcttcttc cgggttatca gcgttttcga
     2341 tttctcgttt aaatattata ttcgctgctc cgtcaggtcc cattacagca atttctgctg
     2401 acggccatgc caatacaaca tctgccccta attcttttga acacattcct aagtatgcac
     2461 cgccatatgc ttttcttaat attagtgtaa cctttggaac tgtagcttcg gaatatgcaa
     2521 agagcatttt tgctccatgt cttataattc ctccgtgttc ttgtgcagaa cccggtaaaa
     2581 atccaggcac gtcaaccaaa tttaacaacg gaatattgaa gcaatcgcat gttctgataa
     2641 atctgctcga tttgtccgat gcatctatat caaggcatcc tgctaaatgt ctgggctggt
     2701 ttgctataac tcctattgtc tgtccgttta ttcttatgaa tccagttaca atattcttag
     2761 catagtatgg ctgtgtttcc aagaaatcgt tattgtctgc tatagtttgt attatttctt
     2821 ttacgtcgta tgccttgttt ggattttcag gaactatgtc atttaatttt tcttctactc
     2881 tgtttaaatc gtcattcgtt tccaccagcg gtgatgtttc caaattgtta gacggcaaaa
     2941 agcttaatag tctttttatt tcctgtatgc aagcttcatc gtttgcactt acaaaatgag
     3001 ctactccgct tattgcattg tgtgccatcg ctccgcctaa tgcttccggg ctgatttctt
     3061 ctccggttac agccttaatt acctgcggtc ccgtaataaa catctggctc gttttatcta
     3121 ccataaaggt gaagtctgtt attgcaggtg aataaacggc tcctccggcg catggaccca
     3181 ttattgccga tatttgcgga attactcctg atgaaattgt atttctgtaa aatatgcttc
     3241 cgtatccgct caaggcatcc acgccttctt gtattcttgc tccgcctgaa tcattcatac
     3301 cgattatggg tgctcccatc ttcaaagcta aatccataac tgtagtaatt ttctttgcat
     3361 gcattgctcc aagggatccg ccaagtaccg tgaagtcttg agcatatgca taaaccagtc
     3421 ttccatcaac tgttccataa cccgttacta caccttcgcc gggggtttcc acttcttcca
     3481 tcccaaaatc tgtacatctg tgttttacaa ataatcctat ttctctaaaa cttccctcat
     3541 caaacaatag gctcaatctt tccctagctg tcaactttcc ctgtgcatgc tgcttttcta
     3601 ttcttttagg tccgccgcct tgttttaatt tggattttct actgtatact tcttctgttt
     3661 tattagacat tttttacctc caaagtattg agcttagtgc ctgcaagaca ctctcattct
     3721 tttgaactcc tgttagaatt gcttgattct ataaaactta tgatacctct ttgatacgta
     3781 tatcaatttc taatatattt atctcctcct ctcacaatac tattccggtt attgataatt
     3841 ttcatcaaga gtttgcttta aacgttatta tcgttttcta ctgtcttaat cagtctcttt
     3901 ggcagagctc tataagaact ccaaatgtag acttaggatg gcagaatgct atcttagctc
     3961 ctccggctcc gtagcgcggc ttctcgtcta tcattctgat tccattttct ttcatttctg
     4021 ctattgcttt ttctatatca tcaactctgt aagctatatg ctgaatacct tcaccgtttt
     4081 ttgcaatata tcttgcaatt ggtccgtctt cttctgttga ctccaaaagt tcaacctcag
     4141 tatctcctaa tggaaggaaa gctaccctaa ctttttgctc ttccactgtt tctgttccgg
     4201 ctgattttaa tccaaggata ttttcgtaga aatttaacgc ttcatctaaa ttttttacgg
     4261 caatacctat atgatctact tttttaacca taattacctc cttca
//
LOCUS       DAOPUE010000145         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_20679_length_4293_cov_8.399009, whole genome shotgun sequence.
ACCESSION   DAOPUE010000145 DAOPUE010000000
VERSION     DAOPUE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20679_length_4293_cov_8.399009"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1209)
                     /locus_tag="PKH42_09350"
     CDS             complement(43..1209)
                     /locus_tag="PKH42_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOA20681.1"
                     /translation="MKIAIAGTGYVGLSIAVLLAQHNEVIALNRTQEKADMINNNKSP
                     IVDTEIEEYLANRSLNLRATTDSNAAYKNAEYVIIATPTNYDENKNYFDTSSIEEVIS
                     NVLSTNPHTVMVIKSTVPVGYTEKVKEMFNTDNIIFSPEFLREGKALYDNLYPSRIVV
                     GEKSERAETFANLLAEGAIKKDIPILYTDSTEAEAIKLFANTYLALRVSFFNELDTYA
                     EVRGLNTHQIIQGVCSDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYTDVPQNIMAAV
                     VDANRTRKDHIADMIIRKKPRIVGIYRLTMKTDSDNFRQSSIQGVMKRIKGKGIEVVV
                     YEPALKDDNFYNSRVIKDLVEFKKISDVIVANRMSDDIKDVADKVYSRDLYSRD"
     gene            1528..3384
                     /locus_tag="PKH42_09355"
     CDS             1528..3384
                     /locus_tag="PKH42_09355"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA20682.1"
                     /translation="MQRAILSKKNQITIVSGILLIIALFGHFVLKSEEIVVSSLVIAS
                     VIGVAPIAIQAYQALRVKVVSIDVLVTIAVTGAFIIRNFEESAIVTFLFLFGAYLEQR
                     TLNQTRSAIKELTEMAPESALKQKEDGEFEEVEVDEVDVGDILLVKTGAKVPVDGTVL
                     SGEGHINEASITGESVPVSKWKDTQVYAGTILENGTLQIVADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLSFIVWLFSKDIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGAKHGVLLKGSEVFQDFSRVDTIVFDKTGTLTIGNPKVADKEIYADNPY
                     EIDEILAYLSSVEKESDHPLAKAVVEYIGDTKVYEVQKTDVVKGGGIVAEVDGYRVAV
                     GNVSLMEQENVHLNERVLLDIAKFERNGNSLVLTSVDGQLKALMGIRDQIRPGVKEDL
                     LKLKKLGVKNLVVLSGDNQGTVDLVARELGLTEAHGHMLPEDKSVYIKELIDKGHIVV
                     FVGDGVNDSPSLALAHIGIAMGSGTDVAIETSDVVLMNSDFSRLPHALGLTKATSRNM
                     LQNIFIAVGVVLILLASVFFSDWMNMSIGMLVHEGSILVVILNGMRLLRYKL"
     gene            complement(3438..4010)
                     /locus_tag="PKH42_09360"
     CDS             complement(3438..4010)
                     /locus_tag="PKH42_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOA20683.1"
                     /translation="MKIVCLGDSLTYGYGVRRSKIWTKLAADKLNIEIINEGINGDTS
                     SGMLSRFYNSVYMQGPEAVHIMGGINDLIAGADIGFIKANIMAMVHQATAKCIKPIIG
                     IPVKMDAKNVKQEWAEFADFYKISAEFEDYYKWMLKFCKAFNIEYIDYYTKLEKLKGA
                     EDVYLDGLHLNNRGNEIMAEIFCHHIQNTR"
BASE COUNT         1308 a          757 c          861 g         1367 t
ORIGIN      
        1 ttcaccaagg ggcacagcta ggtgcaacct gccactctcc cactaatccc tgctatacaa
       61 atcccttgaa tataccttat ctgctacatc cttaatatca tcagacattc tgttagcaac
      121 aatgacatcg gatatttttt taaactcaac taaatcttta attactctag aattgtaaaa
      181 gttgtcatct ttaagagccg gctcgtacac tactacttct atacctttac ctttgattcg
      241 cttcatgact ccttgaattg aagattgcct gaagttatcc gaatcagtct tcatggttaa
      301 tctataaata ccaacaattc tcggtttttt cctgataatc atatcggcaa tatggtcttt
      361 tcttgtacgg ttggcatcta ccaccgctgc cattatattt tgcggtacat cagtataatt
      421 agctaaaagc tgcttggtgt cctttggtaa acaatatccg ccataaccaa atgatggatt
      481 gttgtaatga ttccctattc gcgggtcgga gcacactcct tgaataattt gatgtgtgtt
      541 taatcctcta acctctgcgt aagtatctaa ttcattgaaa aatgaaactc ttaatgcaag
      601 gtaagtatta gcaaatagct tgatggcttc cgcctctgta gaatcagtat aaaggattgg
      661 gatatctttc ttaatggcgc cttcagccaa tagatttgca aaagtctcag ctctttcaga
      721 tttctctcct acaacaattc ttgaaggata aagattatca tataaggcct ttccttctct
      781 taaaaattcc ggagaaaaga tgatattatc agtattaaac atttctttta ccttttcagt
      841 atatccaacc ggcacagttg atttaataac cattactgta tgaggattag tacttaatac
      901 atttgaaata acctcttcaa tagagctggt atcaaaataa tttttatttt catcataatt
      961 ggttggtgtt gcaataatta catattctgc atttttgtat gccgcattgc tgtcagtggt
     1021 tgctcttaaa tttaaacttc tgtttgccaa gtattcttct atttcagtat ctactatggg
     1081 tgatttatta ttatttatca tatctgcttt ttcttgagtc ctgttcaatg caattacctc
     1141 attatgctgt gctaagagca ctgctataga taaacctacg taaccagttc ctgctattgc
     1201 tattttcatt ttgtatactc ctcctgttat agtaaacgta ttccttatat tatcttatat
     1261 taaatttatg caattttcaa gtatataaac aaatattgtc ataatttatc aaatgccttg
     1321 actctcctca tatatcaaaa gagagtcaag tcaatagaat aaaaatcttt ttaaattatt
     1381 gatttaaatc aattcatatt ccttagatta ttggtacaat acaattaata aataagtaaa
     1441 ctgatcggtg cctatcactg ataggagcgc aaagtaacat ataatatgag ttaaatcagg
     1501 agaaaataat ttaaccggga ggagaatatg caaagagcaa tattaagtaa gaaaaatcaa
     1561 ataactatag taagcggaat cttattaata atcgcattgt tcggccactt cgttttaaag
     1621 agtgaagaaa tagtagtgtc gtccctggtt attgcttcag ttataggggt tgcgccaatt
     1681 gcaattcagg cttaccaggc attgagagta aaggttgtaa gcatagatgt gctggttacc
     1741 atagctgtta cgggagcatt tataatcagg aattttgaag agtcggccat tgttactttc
     1801 ctgttcttgt ttggagcata tttggagcag agaacgctta atcaaacacg ttctgcaatt
     1861 aaggaattaa cagagatggc accggaaagt gctttgaagc agaaggaaga cggtgaattt
     1921 gaagaagtcg aggtggatga agttgatgtg ggagatatcc ttctggttaa gaccggagca
     1981 aaggttcctg ttgacggtac tgtcttatct ggagagggcc acatcaatga agcaagcata
     2041 accggtgaat ctgttccagt aagcaagtgg aaagatactc aagtgtatgc gggaaccata
     2101 ttggaaaatg gaacccttca aattgttgct gaccgggtag gcgaggatac taccttcggg
     2161 aagattattg agctggttga agaagcacag gattctaagt cagaagctga gcgtttcatt
     2221 gacagattct caaaatacta cacaccggct gttttggtcc tgtcatttat agtatggttg
     2281 ttttcaaagg atattgaact tgcaattaca atattggtac ttggatgtcc gggggcactg
     2341 gttatcggcg tacctgtttc aaatgttgca ggcatcggca atggagcgaa gcacggtgtt
     2401 ttattgaagg gcagcgaagt attccaagat ttcagcagag tcgataccat agtatttgat
     2461 aaaaccggta cattgacgat aggaaatcct aaggttgccg acaaggaaat ttatgcggat
     2521 aatccttatg aaattgatga gatattggct tacctgtcca gtgtcgaaaa agaatcagat
     2581 catcctttgg ctaaagcagt tgtggaatat ataggtgata ccaaggttta tgaagttcaa
     2641 aaaacagatg ttgtaaaggg cggcggaatt gtagccgaag ttgacggcta tagagtagct
     2701 gtgggtaatg tttccctgat ggagcaagaa aatgttcatt taaatgaaag ggtgcttctg
     2761 gatattgcaa agtttgaaag gaacggaaac tctcttgttc tcacatcggt tgacggccag
     2821 ttgaaggcgc tgatgggtat tcgtgaccag atacgccccg gagtaaaaga agatttgctg
     2881 aaacttaaga aattaggtgt taaaaacctg gtagttcttt cgggagacaa ccagggaaca
     2941 gttgatttgg tagcaaggga attggggctt acggaagctc acgggcacat gctgccagaa
     3001 gataagtctg tatatatcaa agagcttatt gataaaggcc atattgtggt atttgtggga
     3061 gacggagtaa acgacagtcc ttcactggct ctggctcata ttggaattgc catggggagc
     3121 ggtacggacg tagcaatcga aacatcggat gttgtgctta tgaattctga tttcagccgt
     3181 ctgcctcatg cattgggctt gacaaaagca acctcaagaa acatgctcca aaacatattt
     3241 atagcggtag gtgtagttct gattctgctt gccagtgtat tcttcagcga ctggatgaac
     3301 atgtccatag gaatgttggt gcacgaaggc tcaatattgg tggtaatact aaacggcatg
     3361 aggctgctca ggtataaatt gtgatggcta tagagtgctt tagatatgat ggctctcagt
     3421 tattttgaat ataataacta tcgtgtattt tgaatatgat ggcagaaaat ttctgccatt
     3481 atttcattgc cccgattatt aaggtggagg ccgtctaaat acacatcttc cgcacctttt
     3541 agtttctcca gtttcgtata ataatcaata tattcaatat taaaggcttt gcagaatttc
     3601 agcatccact tgtaataatc ttcaaattca gctgatattt tataaaaatc agcaaattct
     3661 gcccattcct gctttacgtt ttttgcatcc atcttgacag gtatgcctat aattggcttg
     3721 atgcacttgg ctgttgcttg atggaccatt gccataatat tggcttttat aaaaccaatg
     3781 tctgctccgg caatcaaatc attgataccg cccataatat ggactgcctc aggcccttgc
     3841 atgtaaactg aattgtaaaa tctgctcagc attccgcttg atgtgtctcc gtttattcct
     3901 tcatttataa tttcaatatt cagcttatct gcagctaatt tagtccatat ttttgacctt
     3961 ctaacgccgt agccataggt taagctgtca ccgagacata ctattttcat attttcactt
     4021 ccaatctgtg tgatttgcag atattatacc ataaaaatta gagaacagtg gaaaaattga
     4081 tttaaatcaa tttaaatggt gtaattatag ggtataataa gagtataagt aagttatagg
     4141 gacacacctc taattaaggg aaagcctcag agtaggagca ttaaaagaat gatcccgcac
     4201 ttgaaccagg aatgcctttg aaatataaaa atataaaaaa taaaggagag atgaaaatgc
     4261 aaaaagcaac tattcaatta gaaacattaa ctt
//
LOCUS       DAOPUE010000146         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_21049_length_4238_cov_13.030122, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000146 DAOPUE010000000
VERSION     DAOPUE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21049_length_4238_cov_13.030122"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /gene="corA"
                     /locus_tag="PKH42_09365"
     CDS             complement(<1..963)
                     /gene="corA"
                     /locus_tag="PKH42_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOA20684.1"
                     /translation="MNELENKSEGTKEIESPQNVKVDNYTIEIIEYNPDNYNEYNIVG
                     NLLHDYQLMNDIPEGSTRWINIDGEYTEETLKRICSICSIHPLIYDNILNNNQRAKVE
                     DYGDYLYIVSKMIFYLDGELIFEHICFILGKNFIISFGETKGDVFDDIRNRIRSEGTN
                     IRKLKADYLMYSIFDAIIDGYFDVLEALGEKIDDVEENVMKISNPEDMHEIRSIKKSL
                     LRIHKYVWPLREVASWLSNESTELIHPNTDLYIRDLRDHIMQVIDATESYRELLAGLA
                     DLYISNISYRLNEVMKVLTIISTIFIPLTFIAGVYGMNFKYMPEL"
     gene            complement(1072..1145)
                     /locus_tag="PKH42_09370"
     tRNA            complement(1072..1145)
                     /locus_tag="PKH42_09370"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1109..1111),aa:Val,seq:gac)
     gene            1582..3429
                     /locus_tag="PKH42_09375"
     CDS             1582..3429
                     /locus_tag="PKH42_09375"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOA20685.1"
                     /translation="MQRAILSKKNQITAVSGILLIIALFSHFVLKSEEIVVTSLVIAS
                     IMGAAPIAIQAYQALRVKVVSIDVLVTIAVMGAFFIRNFEESAVVTFLFLFGAYLEQR
                     TIIQTRSAIKELTEMAPESALKQMEDGEFEEVEVDEVDVGDVLLVKTGAKVPVDGRVL
                     SGEGHINEASITGESVPVSKRKDTQVYAGTILENGTLQIVADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLSFIVWLFSKDVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGAKHGVLLKGSEVFQDFSRVDTIVFDKTGTLTIGNPKVADKEIYADETD
                     EVLSYLSSVESESDHPLAKSVVEYIGDTKVYEVEKTDVVKGGGIVAEVGGHRVAVGNV
                     ALMEQENVHLNEKVLLDIARFEKSGNSLVLTSVDGELKVLMGIRDQIRPGVKEDLQKL
                     KKLGVKNLVVLSGDNQGTVDLVSRELGLTEAHGHMLPEDKSVYIKELIDKGRIVAFVG
                     DGVNDSPSLALAHIGIAMGSGTDVAIETSDVVLMNSDFSRLPHALGLTKATSRNMLQN
                     IFIAVGVVLILLASVFFSDWMNMSIGMLVHEGSILVVIINGMRLLRYKL"
     gene            complement(3483..4055)
                     /locus_tag="PKH42_09380"
     CDS             complement(3483..4055)
                     /locus_tag="PKH42_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOA20686.1"
                     /translation="MKIVCLGDSLTYCYGVRRSKIWTKLAADKLNIEIINEGINGDTS
                     SGMLSRFYNSVYMQRPDAVHIMGGINDLIVGADIGFVKANIMAMVHQATAKYIKPIIG
                     IPVKMDVKNVRQEWAEFANFYKVSAEFEDYYKWMLKFCKAFNIEYIDYYTELEKLKGA
                     EDVYLDGLHLNNRGNEIMAEIFCHHAQNTR"
BASE COUNT         1332 a          727 c          829 g         1350 t
ORIGIN      
        1 aagttccggc atatatttaa aattcatgcc gtatacccct gctataaatg taagaggaat
       61 aaaaatagtt gaaattattg taagcacttt cattacttca ttgagccgat aacttatatt
      121 tgaaatatat aaatcagcaa gacctgccaa taattcccta tatgactctg ttgcatctat
      181 tacctgcatt atatgatcac gcaaatctct gatatataag tctgtattag gatgaatcaa
      241 ttctgttgac tcatttgata accaagatgc aacttctcta agcggccata catatttatg
      301 aattcgcaaa agactttttt taatagacct gatttcatgc atatcttccg gattggaaat
      361 tttcataaca ttttcttcaa cgtcatctat tttttctcct aaagcttcca atacatcaaa
      421 atatccgtcg attatcgcgt caaatattga atacatcaaa taatctgcct ttaattttct
      481 tatgttggta ccttcacttc ttattctatt tcgaatatcg tcaaatacgt ctccttttgt
      541 ttctccaaaa gaaataataa agttcttccc taaaataaaa catatatgtt caaatatcag
      601 ctctccatcc aaataaaaaa tcatcttgga cacaatatac aagtaatccc cgtaatcttc
      661 aacttttgca cgttgattat tatttaaaat attatcataa attagagggt gtatactgca
      721 tatactgcaa attcttttca aagtttcctc agtatattct ccatcaatat ttatccatct
      781 ggtgctgcct tccggaatat cattcatcaa ttgataatca tgcaataaat ttcctactat
      841 attatattca ttgtaattat ccggattgta ttctattatc tcaattgtat agttatcaac
      901 tttaacattt tgcggacttt caatttcttt tgtcccttca gacttatttt ctaattcatt
      961 cattacatca cctcactaaa atacagcttt ccttttacac aacaaaaaaa ttcctattac
     1021 aattataatg gaatttttta cattatacaa caaaaaagaa ggagcttttg atggacgata
     1081 ttgggatcga accaacgacc tccacgatgt caacgtggcg ctctaaccag ctgagctaac
     1141 cgtccgataa gtatatgata gcataatagt tttaaatctg caatgaaaaa atgttcagct
     1201 aatactctat ttagttttac atctattagg tcagatatgg ctaataaatc ctctttcgtt
     1261 aagctaatag gcctacctcg tttctgttat tttttaataa atgtatcaat atattttagc
     1321 tatatcaagt caataaaaat attcttaaat ttttgattta aatcaattca tattctttat
     1381 tttcctggta caatgcaatt ataagataag caaacttatt ggagtctggt gttgcctggc
     1441 agcatgagca ggtgctgaaa gcaggtaacg ggaacaggca ctgagagcag gtgcttagag
     1501 caggaacaga gagctgcacc gagtaaactt ataatattat agttgaaaac aggagaatat
     1561 aaataagtca ggaggagaaa tatgcagaga gctatattaa gtaagaaaaa tcaaataacg
     1621 gcggtaagcg gaattttatt aataatagca ttattcagtc attttgtctt aaaaagtgaa
     1681 gaaatagtag tgacgtcctt ggttatagct tcaattatgg gtgctgcacc aattgcaatt
     1741 caggcctacc aggcattgag ggtgaaggtt gtaagcatcg atgtgctggt taccatcgca
     1801 gttatgggtg catttttcat aaggaacttt gaagagtcgg ctgttgttac attcttgttc
     1861 ttgtttggag catatttgga gcagagaacg attattcaaa cacgttctgc cattaaagaa
     1921 ttaacggaga tggcaccgga aagtgctttg aagcagatgg aagacggtga atttgaagaa
     1981 gttgaagttg atgaagttga tgtgggagat gttttgctgg ttaagaccgg cgcaaaggtt
     2041 cctgttgacg gtagagtttt atcaggagaa ggccacatta atgaagcaag cattacaggt
     2101 gaatctgttc cagtaagcaa gcggaaggat actcaagtgt atgcgggaac catattggaa
     2161 aatggaacac ttcaaattgt tgctgaccgt gtaggcgaag atactacctt cgggaaaatt
     2221 attgagctgg ttgaagaagc acaggattcg aaatcagaag ctgagcgttt cattgacaga
     2281 ttctcaaaat actatacacc ggctgtattg gttctgtcat ttatcgtatg gctgttttca
     2341 aaggatgttg aacttgcaat tacaatattg gtattaggat gtccgggagc actggttata
     2401 ggcgtacctg tatcaaatgt tgcaggcata ggcaatggag cgaagcacgg cgttttattg
     2461 aagggcagcg aagttttcca ggatttcagc agggttgata ccatagtatt tgataaaacc
     2521 ggtacattga cgataggaaa tcctaaggtt gccgacaagg aaatttatgc agatgaaact
     2581 gatgaagtat tgtcttacct atcgagtgtg gagagtgaat cagatcatcc tttggctaag
     2641 tcagttgttg aatatatcgg agatactaaa gtttatgaag ttgaaaaaac agacgttgta
     2701 aagggcgggg gaattgttgc cgaagttggc ggtcacagag tggctgtggg taatgttgcc
     2761 ctgatggagc aagaaaatgt tcatttaaac gaaaaggtgc ttctagatat tgcaaggttt
     2821 gaaaagagtg ggaactcgct tgttcttaca tcggttgacg gtgaattaaa ggtacttatg
     2881 ggcattcgtg accagatacg ccccggtgtg aaagaagacc tgcagaagct taagaaatta
     2941 ggtgttaaaa acctggtggt tctttcggga gacaaccagg gaacagttga tttggtatca
     3001 agggaattag ggcttacaga agctcacgga cacatgctgc ctgaagataa gtctgtatat
     3061 atcaaagagc tcatagataa aggccgcatt gtagcatttg tgggagacgg agtaaacgac
     3121 agtccttcac tggccttggc acatattgga attgccatgg gaagcgggac ggacgtagca
     3181 atcgaaacat cggatgtcgt gcttatgaat tctgatttca gccgtctgcc tcatgcattg
     3241 ggtttgacaa aagcaacctc aagaaacatg ctccaaaaca tatttatagc ggtaggtgta
     3301 gttctgattc tgcttgccag cgtattcttc agcgactgga tgaacatgtc cataggaatg
     3361 ctggtgcacg aaggctcaat attggtggta ataataaacg gcatgaggct gctcaggtat
     3421 aaattgtgat ggctatcgag tgctttagat atgatggctc taagttattt tgaatataat
     3481 aactaccgtg tattttgagc atgatggcag aaaatttctg ccattatttc atttccccga
     3541 ttattaaggt ggaggccgtc taaataaacg tcttctgctc cttttagttt ctccagttcc
     3601 gtataataat caatatattc aatattaaag gctttgcaga atttcagcat ccacttgtaa
     3661 taatcttcaa attcagctga taccttataa aaattagcaa attctgccca ttcctgtctt
     3721 acgtttttta cgtccatctt gacaggtatg cctattatgg gcttgatgta tttagctgtt
     3781 gcttgatgga ccattgccat aatattggct tttacaaaac cgatatctgc tcctacaatc
     3841 aaatcattga taccgcccat tatatgtact gcatcaggtc tttgcatgta aactgaattg
     3901 taaaatctgc tcagcattcc gcttgatgtg tcaccgttga ttccttcatt tataatttca
     3961 atattcagct tatctgcagc taatttagtc catatttttg accttcttac tccatagcaa
     4021 taggttaagc tgtcgcctag acatactatt ttcattattt cacttccaat ctgtgtgatt
     4081 tgcagatatt ataccacaaa aacttaagaa aagtggaaaa attgatttaa atcaatttaa
     4141 atggcgtaat tatagggtat aataagaata gaagaaagta aagaataaag gagagatgaa
     4201 aatgcaaaaa gcaactattc aattagaaac attaactt
//
LOCUS       DAOPUE010000147         4219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_21177_length_4219_cov_8.599664, whole genome shotgun sequence.
ACCESSION   DAOPUE010000147 DAOPUE010000000
VERSION     DAOPUE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21177_length_4219_cov_8.599664"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKH42_09385"
     CDS             <1..200
                     /locus_tag="PKH42_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOA20687.1"
                     /translation="TGEIVSYQAGLGGTGAIVTGGLEMSNVDLANEFSEMIVTQRGFQ
                     ANTKIITVVDQMLEELVNLKR"
     gene            440..640
                     /locus_tag="PKH42_09390"
     CDS             440..640
                     /locus_tag="PKH42_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOA20688.1"
                     /translation="MVEVVGINGEAFLINAALIEKIEFIPETKITLTTGKYYLVKDSK
                     EEIINKIITFNQKIIKGVTAVE"
     gene            651..1511
                     /locus_tag="PKH42_09395"
     CDS             651..1511
                     /locus_tag="PKH42_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOA20689.1"
                     /translation="MNLSFLIGMLAGVGLVFYGILGSGSSTTQVMTSFIDIPSMYITF
                     GGAIAATIMSVPIKYLKDVPKNLKVLMKKEKIDLNVYIDIIEELAKQARLKGLLVLEE
                     KVNQMDLQDEFLKYCVMLIVDAIEPTKVKAQIENEIDCIEARHASAWKVFDTLGSFGP
                     AFGMLGTLIGLINMLANMDAGEGADALGKGMSVALVTTFYGSVLTNMICAPISNRLKA
                     KHDEEMIAKELIMEGVLSIQSGENPKYIREKLNSYVTYQERSTMAGAEGEGKSKKRSK
                     RKGKEKEANA"
     assembly_gap    1632..1731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1732..2087
                     /locus_tag="PKH42_09400"
     CDS             <1732..2087
                     /locus_tag="PKH42_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOA20690.1"
                     /translation="VDEYIEEVVIAGHTAKVEYDMTDIDRSLSTNRANVVLRYLENKD
                     VIDPAKYLAIGYGLYSPIADNSTAEGRAKNRRVEIYISRKGHPISYTRIIQETINNGK
                     TDNNDLEYRRDIYIE"
     gene            2115..3095
                     /gene="fliM"
                     /locus_tag="PKH42_09405"
     CDS             2115..3095
                     /gene="fliM"
                     /locus_tag="PKH42_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOA20691.1"
                     /translation="MAEVLSQSQIDELLNGLSTGDVSIQELEENEKRIKEYDFRSPKK
                     ITKETIRLLKGIYENYCRMLTSRLTSMLRLMCDVTVEQVEESIYHEYNNALEDYVLMG
                     LINVKYPDNETSDSQILMEMSRPLSFVIIDRLLGGIGEEYGYIRDYTDIELSLLSNVL
                     NQLVILMGTTWENTLNIETELEKVETNSRFIQSINYNDTVVIIILNVVLNELSGKITL
                     CIPSNIIDEILKGANIYSKNISKKVNIEEQKTAIMNSLKSSYLTITGVLGSTQATLKD
                     ILSIKPGDLIVLDRKINSNVDIKVDNEKWFEGKWGTRKNKGVIKINKTIY"
     gene            3107..4204
                     /gene="fliY"
                     /locus_tag="PKH42_09410"
     CDS             3107..4204
                     /gene="fliY"
                     /locus_tag="PKH42_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HOA20692.1"
                     /translation="MLNRKIFSNMERDVIGEVMNISLGSSATSLSSILGKRVEITVPH
                     INVTTAQEFSYKQLEPAIGVEINYIEGLKGVNVMIWKRSDAKIILEALLNKKIPDEEF
                     EMDEISISAICEVMNQMMGSAATALSEFLGKHINISTPIAFEIDDNNEFKSKYFAEDD
                     EIVVVSFDLSIEGELQSQFINVMEVSLAKDIVSNFLRETEKSTSNEIQIEENNNIEKE
                     DAVNKKIDTIIKQNNDYEEIIKRERMKTNQNQKEKEETFVNVRPVEYESFDDEEEVLS
                     EEQLSNLELIMMVPLTISVEIGKTSKQIKDILEISPGTIIELDKQAGALVDIIVNGKL
                     IAKGEVVVVNDNFGVRVVEILKKDELMKITL"
BASE COUNT         1617 a          550 c          873 g         1079 t
ORIGIN      
        1 acacaggcga aatagtaagt tatcaagccg gtctgggcgg taccggtgca attgtaaccg
       61 gaggccttga aatgtccaac gttgatttgg ctaatgaatt ctcggaaatg attgtaactc
      121 aaaggggatt ccaggctaat acgaagatta ttactgttgt tgaccaaatg cttgaagaac
      181 tggttaattt gaaaagataa ataaataagt aacgaaacag cgaaaggggg gttctgtcca
      241 taggggacgg ttctcggtgg acagcaaaat gtccaagaag aaccgtcccc ggtggaccgg
      301 aatgacggga actgcactta gaataaaaat atttattatg ctcataagtg tatcttatgg
      361 atataattaa taattttata ggaatgccta tccccggaga ctatgcctaa aggagaggtg
      421 tccccaataa ggaggaacta tggtagaagt tgttggaatc aacggagaag cttttttaat
      481 caatgctgca ttaattgaaa aaatagaatt tattccggaa acaaagatta cattgacaac
      541 aggaaaatac tatttggtta aagattcaaa agaagaaata ataaataaaa taattacatt
      601 taatcaaaaa ataataaaag gcgttacggc tgtagagtaa ggagtgcgac atgaatttat
      661 cgtttttaat tggtatgttg gcaggagttg gtctggtatt ttacggaata ttaggcagcg
      721 gttcaagtac tacacaagta atgacaagct ttatcgatat accaagtatg tacataacat
      781 ttggcggagc catagctgcg acaattatgt ctgttccgat aaagtatctg aaagacgtac
      841 ctaaaaactt aaaggtttta atgaaaaagg aaaaaatcga tttaaatgtt tacattgaca
      901 taatagagga gcttgctaaa caagcaaggc tgaaaggcct tttggttctt gaagaaaagg
      961 taaatcaaat ggacttgcag gatgagtttt taaagtattg cgtcatgctt atagtagatg
     1021 ctattgagcc tacaaaggta aaagcacaaa ttgaaaatga aatagattgt atagaagcaa
     1081 ggcatgcttc tgcctggaag gtttttgata cacttggctc atttggtccc gcattcggta
     1141 tgcttggaac attaatcgga cttataaata tgcttgcaaa catggatgca ggcgaaggag
     1201 cagatgcgct aggaaaagga atgtcagtgg ccttagttac taccttttac ggctcggtac
     1261 ttacaaatat gatttgtgct cccatatcaa acaggttaaa agcaaagcac gacgaagaaa
     1321 tgattgctaa ggaattgata atggaaggtg ttttatccat acaatcggga gaaaacccta
     1381 aatatataag agaaaaatta aattcatacg taacatatca ggaaaggagc accatggcag
     1441 gggcagaagg ggaagggaaa tccaaaaaaa ggtcaaaaag aaagggaaaa gaaaaagaag
     1501 ctaatgctta ataatagaaa tgagtaggat attatggcga gaagaaagaa aagagaagaa
     1561 agcggcggag caagctggct ggaaacatac agcgatatgg taaccttatt gttaaccttt
     1621 tttataatgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggtcgacg
     1741 aatatataga ggaagttgtt atagcaggtc acactgctaa ggttgaatat gatatgacgg
     1801 atattgacag gtcattatct acgaacagag ccaatgttgt tctaagatat ttagaaaata
     1861 aggatgttat agaccctgct aaatacctgg caataggata tggattatac agtcccatag
     1921 ctgacaacag tactgctgaa ggcagggcta aaaacagaag agttgaaata tacataagcc
     1981 gaaaaggaca ccccataagt tatacaagaa taattcaaga aactatcaat aacggcaaga
     2041 ctgacaataa tgatttggaa tatcgaagag acatatatat agaatagtat aaaattatgg
     2101 aatgaggatt aaatatggcg gaagtactat cacaaagtca aatagatgag ttgcttaacg
     2161 gtttgagcac aggtgatgta agtatacaag aattagagga aaatgagaaa agaattaaag
     2221 aatatgattt cagaagccct aagaaaatca caaaggaaac cataagattg ctaaagggta
     2281 tttacgaaaa ctactgcaga atgcttactt caagattaac atcaatgctc aggctgatgt
     2341 gcgatgtaac ggtagaacag gtggaagaaa gcatatacca tgaatacaac aatgccttag
     2401 aggattatgt attaatgggt cttatcaatg taaagtatcc ggataatgaa acttctgaca
     2461 gccaaattct tatggaaatg tccagaccct tgtcctttgt cattatagac aggcttcttg
     2521 gaggaatcgg agaagagtac ggatatatta gagattacac ggacatagag ttgtcattgc
     2581 tcagcaatgt attaaatcag ttggttattc taatgggaac aacatgggaa aacactttaa
     2641 atatagaaac cgagttagaa aaagttgaaa ccaactccag atttattcag tcaataaatt
     2701 acaatgatac cgtagtaata ataatattaa atgtagtatt aaatgagctg tcagggaaaa
     2761 tcaccttatg cataccttca aacattattg acgaaatact gaaaggtgca aatatctatt
     2821 caaagaatat aagtaaaaaa gttaatattg aagagcaaaa aacagcaatt atgaattcat
     2881 taaaatcatc atacttaact ataaccggag tgctcggaag tactcaggca acattgaaag
     2941 atatactcag tattaagccg ggagatttaa tcgtacttga tagaaaaata aattcgaatg
     3001 tagatataaa agttgataat gaaaagtggt ttgaaggcaa atggggaaca aggaagaaca
     3061 agggagtaat taagataaat aaaactatat actaagggag ttatttatgc taaatagaaa
     3121 aattttttca aatatggaac gagatgtaat aggggaagtt atgaatataa gcttaggctc
     3181 atctgcaact tcattatcct caatattagg caaaagagtt gaaataaccg ttccgcatat
     3241 taatgttaca actgctcaag agtttagcta taagcagtta gagccggcca taggagttga
     3301 aataaactat atagaaggct taaaaggcgt aaatgtaatg atatggaaaa gatctgatgc
     3361 caaaataatt ttggaagccc ttctaaacaa gaaaataccg gatgaagaat ttgaaatgga
     3421 tgaaatcagc ataagcgcca tttgcgaagt aatgaaccag atgatggggt cagctgctac
     3481 agccctatca gagttcttag ggaaacacat aaacatatca acaccgattg cttttgaaat
     3541 tgacgataat aatgaattta aaagtaaata ctttgcagag gatgatgaaa ttgttgtagt
     3601 cagttttgat ttatctattg aaggagaatt gcaaagccaa tttattaatg tcatggaggt
     3661 ttcattagca aaagatatag tatcaaattt tttacgggaa accgaaaaaa gtacaagcaa
     3721 cgaaatacaa atagaagaaa acaataatat tgaaaaagaa gatgccgtaa ataaaaaaat
     3781 tgacacgata atcaagcaaa ataatgatta tgaagaaata ataaaaaggg agcgtatgaa
     3841 aacaaatcaa aatcaaaagg aaaaagagga aacattcgta aatgtaagac ctgtagaata
     3901 tgaatcattt gatgacgaag aggaagtatt atcagaagag cagctttcta atttggaatt
     3961 gataatgatg gttcctttga ctatcagtgt tgaaatcgga aaaaccagca aacaaataaa
     4021 ggatattctt gaaatttcac cgggaacaat tattgagctt gataaacagg ccggagcatt
     4081 agtagatatt attgttaacg gcaagttaat tgcaaaaggt gaagttgttg tggtaaatga
     4141 taattttgga gtaagagtag ttgaaatatt aaaaaaagac gaattaatga aaataacatt
     4201 ataattgaaa tattcaaaa
//
LOCUS       DAOPUE010000148         4123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_21825_length_4123_cov_12.118486, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000148 DAOPUE010000000
VERSION     DAOPUE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21825_length_4123_cov_12.118486"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="gatB"
                     /locus_tag="PKH42_09415"
     CDS             complement(<1..977)
                     /gene="gatB"
                     /locus_tag="PKH42_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOA20693.1"
                     /translation="MSYELVAGLETHIELSTKTKIFCQCTTEFGGEPNTHCCPICIGM
                     PGTLPKLNKKVVEYAVKAGIALNCKIRNISKMDRKNYVYPDLPKAYQISQFDKPICYG
                     GYIKLSSGKNININRIHIEEDAGKLVHERGNTYIDYNRGGVPLIEIVTEPDFSSIDEV
                     LEYLEKLRLVMKYIGVSDVKMQEGSLRCDVNISVRKKGEEKLGTRTEIKNMNSLSFIA
                     KAMEYEFERHVDILESGGEIEQETRRYDSELNITESMRGKEDAEDYRYFKEPDLVDII
                     VSEEEIKKLKSELPQLPDEKLHVFINEYSLPEDDAELLIKYKKIAEYFE"
     gene            complement(974..2431)
                     /gene="gatA"
                     /locus_tag="PKH42_09420"
     CDS             complement(974..2431)
                     /gene="gatA"
                     /locus_tag="PKH42_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOA20694.1"
                     /translation="MSKIKYIQKLLKAKEVSCVELTKNYLNNIEKQNKAINAYVNVTG
                     EEAIKTAIKVDEKIAAGEDLMPLEGIPMSLKDNISTKDIETTCCSKMLKGYYPIYDAT
                     VWEILKNQNSVLLGKTNMDEFAMGSSCETSIFGTTLNPHNTDYVAGGSSGGAAASVAG
                     NMAAFGLGSDTGGSIRQPASFCGIVGFKPTYGTVSRYGVVAYASSLDQVGPITDSVED
                     AAIVYDCLSLYDPKDSTSIGSKTGTCDKLHNSIKGLKIGIADEYFEGINEDIISSIEE
                     AIKVYQDAGAEIVNFEIPKLKAFLPVYFIIACAEASSNLGRFDGIRYGYKTDSYNDVN
                     DMICKNRSEGFGKEVQKRILLGTYALSSGYYDAYYKKAQNLRYSIIQDFKNIFKSCDV
                     IITPSVPVTAFKKGEALTDPIATYMMDICTVPANIAGLPAISVPCGFDKNKMPIGMQI
                     IGNSFEDYKVFNAAYQYEKQTKKSDEISPYPEVKI"
     gene            complement(2436..2729)
                     /locus_tag="PKH42_09425"
     CDS             complement(2436..2729)
                     /locus_tag="PKH42_09425"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOA20695.1"
                     /translation="MITKEEVLKIAKLAKLSVNNDEIDMLIEDMSKIIEYADAINTVV
                     EDEDNEVEFYNINNSANAFNEDIVIDSYDRDEILKNREGGEKGYFVIRKRHRG"
     gene            complement(2738..>4123)
                     /gene="aspS"
                     /locus_tag="PKH42_09430"
     CDS             complement(2738..>4123)
                     /gene="aspS"
                     /locus_tag="PKH42_09430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOA20696.1"
                     /translation="EDLRLKYRFLDLRNVEVQKNIILRSKVISFMRSKMTEMGFLEIQ
                     TPILTSSSPEGARDYIIPSRKHPGKFYALPQAPQVFKQLLMVSGFDKYFQIAPCFRDE
                     DARADRSPGEFYQLDFEMSFATQEDVFSVAEEVLYDTFTKFSDKQVSKPPFKRIPYEE
                     SMLIYGTDKPDLRNPLTIIDISDMFEETDFAPFRKKTVRAINVPECSSQPKRFFEEML
                     EFATKIGMKGLGYIKVNDEMEYQGPIDKYLSHEDRKELAERARLKPGDVLFFIADTRE
                     EAPKLAGQIRAELAKKLKLIDENRYEMCYIVDFPMYEIDEKTGGIAFTHNPFSMPQGG
                     LKSLMEKDPLDIKAFQYDIVCNGIELSSGAVRNHDLDIMIKAFEIAGYTIDDLQTKFT
                     SLFNAFKYGAPPHAGMAPGIDRIIMLLTGEENIREVIAFPMNNNAQDLLTNAPSKVTE
                     QQLREVHIKLR"
BASE COUNT         1115 a          873 c          638 g         1497 t
ORIGIN      
        1 tcttcaaaat attcggcaat ttttttgtat tttatcaaaa gctccgcatc atcctccgga
       61 agactgtatt cattaataaa tacatgcaat ttttcatccg gcagctgagg aagttcactt
      121 tttaattttt ttatttcttc ttcggaaact attatatcga ctaaatcagg ctctttgaaa
      181 tacctgtagt cttcggcatc ttcttttcct ctcatacttt cggtaatatt aagctcggaa
      241 tcatatcttc tcgtttcctg ctcaatttca ccgccgcttt ctaaaatgtc cacatgcctt
      301 tcaaattcat attccattgc tttggctata aagctgaggg agttcatatt tttaatctcc
      361 gtcctggtgc caagcttttc ttctcccttt ttccttaccg atatattaac gtcacatcgc
      421 agtgagcctt cctgcatttt gacatccgat acgcctatat acttcataac cagtcttaac
      481 ttttccaaat actcgagaac ttcatcaatg ctgctaaaat cgggctccgt tacaatttcg
      541 ataagaggta ctccgcccct gttgtaatca atataagtat ttcctctttc atggactaac
      601 tttcctgcat cttcttctat gtgtatcctg ttaatattaa tatttttgcc tgatgaaagt
      661 ttaatatatc caccataaca aataggttta tcaaattgag atatttggta tgcttttggt
      721 aaatcaggat atacataatt tttacggtcc atttttgaaa tatttcttat tttgcagttt
      781 aaggcaatgc ctgcttttac ggcatattcc acaacctttt tattaagttt gggaagagtg
      841 cccggcattc ctatgcatat gggacagcaa tgagtgttag gctcccctcc gaactctgtc
      901 gtgcattgac agaatatctt agtcttagta gatagttcta tatgtgtttc taatcctgca
      961 actaattcat aactcatatt ttcacctccg gataggggga tatttcatct gacttttttg
     1021 tttgcttctc gtattgatac gctgcattaa acactttata atcttcaaag ctgttgccga
     1081 ttatttgcat ccctatgggc atcttatttt tgtcaaagcc gcatggaacc gaaattgcag
     1141 gtagaccggc aatgtttgcc ggaacagtac aaatatccat catataagtt gctatggggt
     1201 cggttaaagc ttctcctttt ttaaatgctg tcaccggcac tgaaggagta ataataacat
     1261 cacaactttt aaatatattt ttaaaatctt gtataattga atatcttaaa ttttgcgcct
     1321 tcttataata tgcatcgtag tatccggaac ttagcgcata agtaccaagc aaaattcttt
     1381 tttgcacttc cttgccgaaa ccttcactcc tgtttttgca tatcatatca tttacatcat
     1441 tgtaactgtc tgttttatat ccgtatctta taccatcaaa tcttcctaaa ttagatgaag
     1501 cttcagcgca cgcaataata aaataaaccg gaaggaaagc tttcagctta ggtatttcaa
     1561 aatttactat ttctgcaccc gcgtcttggt aaacttttat tgcttcctct attgaactta
     1621 taatatcttc atttattcct tcaaaatact catctgctat tcctattttt agtcccttga
     1681 tactattatg taatttatca catgtaccgg ttttgcttcc tatggatgtt gagtccttag
     1741 ggtcgtataa ggacaggcag tcgtatacta ttgcagcatc ttccacagaa tcggttattg
     1801 gtcccacctg gtccaagctt gaagcataag caacaactcc atatcgggaa accgtgccat
     1861 acgtaggttt aaatcctaca atgccgcaaa agcttgcagg ctgccttatg gaacctcctg
     1921 tgtcggagcc taagccgaat gctgccatat tccccgctac ggaagctgca gctcctccgg
     1981 agcttcctcc tgccacataa tccgtgttgt gaggattcaa tgttgttccg aagattgaag
     2041 tttcacaaga tgagcccatg gcaaactcat ccatattggt ctttcccaga agcacggagt
     2101 tttgattctt taatatctcc caaacagtag catcataaat aggataatat cctttaagca
     2161 tttttgagca gcatgttgtt tcaatatcct tggtagaaat attgtctttt aaagacatgg
     2221 ggatgccttc caaaggcatt aaatcttctc cggctgcaat tttttcatca acctttattg
     2281 ctgttttaat tgcttcttca cctgttacgt tgacatatgc atttatagcc ttattttgtt
     2341 tttcaatgtt gttaaggtag ttttttgtca gttcaacaca tgaaacttcc tttgctttta
     2401 ataatttttg tatatattta attttgctca atctttcacc ccctatgcct tttccttatt
     2461 acaaaatatc ccttttctcc gccttctctg ttctttaaaa tttcatccct gtcataagaa
     2521 tctataacga tatcttcgtt aaaagcattt gcactgttgt taatgttata aaattcaact
     2581 tcattatctt catcttcaac aactgtatta attgcatctg catattcgat tattttagac
     2641 atatcttcaa tcagcatatc aatttcatca ttatttaccg ataatttagc aagctttgca
     2701 attttcaaaa cttcttcctt agttatcatt atctcaccta cctcaatttt atatgaactt
     2761 ctcttaattg ctgctctgta acctttgatg gtgcatttgt cagtaaatct tgagcattgt
     2821 tgttcatggg gaaagctata acttctcgaa tattttcttc gcctgtaaga agcattatta
     2881 ttctgtcaat tcccggagcc atgcctgcgt gaggaggtgc tccgtattta aatgcattga
     2941 acaaggaggt aaattttgtt tgtaaatcat ctattgtata ccctgctatt tcaaatgctt
     3001 taatcattat atccaaatcg tgatttctta cagctccgga tgaaagttca ataccgttac
     3061 atacaatatc atactgaaaa gctttaatgt caagagggtc tttttccatt aatgatttca
     3121 atcctccctg aggcattgaa aacgggttgt gtgtaaatgc tattcctcct gttttttcat
     3181 ctatttcata cattggaaaa tcaacaatat aacacatttc atacctgttt tcatctatta
     3241 atttcagttt ctttgcaagc tcggctcgta tttgtcccgc aagtttaggc gcttcttctc
     3301 ttgtatctgc aataaaaaac aatacgtctc ccggtttcag tcttgctctt tcagcaagtt
     3361 cctttctgtc ttcatgagaa agatatttat ctatgggacc ctgatattcc atttcatcat
     3421 taaccttgat gtagccaagt cccttcattc cgattttagt tgcaaattca agcatctctt
     3481 caaaaaaacg cttgggctgg gatgagcatt caggcacatt gattgctctt accgtcttct
     3541 ttctgaatgg tgcaaagtct gtttcttcaa acatatcgct tatatcaatt attgtcagcg
     3601 ggtttctcaa atccggtttg tcagtgccgt atataagcat tgattcttcg taaggaatcc
     3661 ttttaaaagg aggttttgat acttgcttat cggaaaactt agtaaaggta tcatacaata
     3721 cttcttcagc aaccgaaaat acatcctctt gagttgcaaa tgacatttca aaatcgagct
     3781 gataaaactc accgggagag cggtcagccc tggcatcctc atctctgaaa caaggagcta
     3841 tctgaaaata cttatcaaac cccgaaacca tcaaaagctg cttaaacact tgaggagctt
     3901 ggggcaatgc ataaaatttt ccgggatgct ttctgcttgg tataatataa tctcttgctc
     3961 cttcaggaga agaagatgtt aaaatcggtg tttgaatttc caaaaaaccc atttctgtca
     4021 ttttgctcct cataaaagat attacctttg agcgtagaat gatatttttt tgaacttcca
     4081 cgtttcttaa gtccaaaaac ctgtatttta atcttaaatc ttc
//
LOCUS       DAOPUE010000149         4106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_21947_length_4106_cov_10.416934, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000149 DAOPUE010000000
VERSION     DAOPUE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21947_length_4106_cov_10.416934"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1919)
                     /locus_tag="PKH42_09435"
     CDS             complement(<1..1919)
                     /locus_tag="PKH42_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOA20697.1"
                     /translation="MMGGLNNSARNALALAQEEVKNFKQNVLGTEHILLGLMIEEESI
                     AGKILRDKLDIDKVREIIKNSTGMGDSVPEYITISPRSKFIIELARQYAAQLKMPYVG
                     CEHILLGLIEEGEGIGAQIIKSVISLKDLKNEVLMAMQGAGGAEPGNQDIGGSMAPQT
                     SNTKTIDKYGTDLNQRAKEGRLDPVIGREDVIERVIQILSRRTKNNPCLIGEPGVGKT
                     AIAEGLAQEIIKGSVPETLKDKRVITLDMAGMVAGAKYRGEFEERLKNAIDEIKAAGN
                     IILFIDEMHTIIGAGAAEGAIDASNILKPVLARGELQVVGATTLDEYKKHIEKDAALE
                     RRFQPIIVEEPTVEDTIRILKGLRDKYEAHHKVAITDEAIEAAASLSHRYISDRFLPD
                     KAIDLIDEAASRIRLKSSTAPKELKELEDKIKEVDTEKRTAINNQDFEKAASLRDEEK
                     KLTDELNKQRKLWAAKNIKDIKINYEDIADVVSQWTGIPVKKLQGDESERLLNMEEIL
                     HKRVIGQEPAVEALSKAIRRSRVGLKDPKKPIGSFIFLGPTGVGKTELSKALAEAMFG
                     DEDAMIRIDMSEYMEKHSVSKMIGSPPGYVGFDDGGQLTERVRRKPYSVILFDEIEKA
                     HPDVFNILLQILDDGR"
     gene            complement(1950..2936)
                     /locus_tag="PKH42_09440"
     CDS             complement(1950..2936)
                     /locus_tag="PKH42_09440"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOA20698.1"
                     /translation="MKNNDIVITSRIRLARNLKNYKFPIYMTESESNSVINEIYNAIS
                     KHENYKLTYMKDLSDVKKNSLIENHIISPALANKRNEGAFLLSPDGKTSVMINEEDHI
                     RIQTIEDGLSLKECWKLSNEVDDIIEESVDYAFDRELGYITACPTNIGTGMRASVMLH
                     LPALSITNQIDKLLYGVSQLGVAVRGVYGEGTKSIGHLYQISNQGTLGASEETLIDKI
                     NQIVMQIIDKEKNTREHLMKNNYDDLEDDFYRAYGLLTNARKMTVDEAMKLLSLIKLG
                     SEMGITPMVKGKNIYQLMIWIQPNNISTIDTELSPKDRDKKRAEIIRRELLR"
     gene            complement(2926..3435)
                     /locus_tag="PKH42_09445"
     CDS             complement(2926..3435)
                     /locus_tag="PKH42_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOA20699.1"
                     /translation="MLCNECGKNEANVHLTHIINGKKTESHLCEECAKKNQTIFNPSF
                     SMQNLFSAMLNNSFNNKTYLPAKGCSNCGMTYENFRTTGKFGCSQCIDSFKGRLKPVI
                     KSIQGYDRHTGKIPKRTGGAYKVQMDIERLKNELRCAVEREEYELAAELRDKIYELEK
                     KKGDEQFEK"
     gene            complement(3454..3927)
                     /locus_tag="PKH42_09450"
     CDS             complement(3454..3927)
                     /locus_tag="PKH42_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOA20700.1"
                     /translation="MSNLSDIIESFIKELLDANNNDVIEIQRNILAQQFDCSPSQINY
                     VLTTRFTNDRGYVVESRRGGGGYIRIFRVRSSMEDELKRILNETIGDSITLNKSIDLI
                     NALMEREVITEGERAIMQSVLSDRVLNIVAYEDRNKLRADLLKEMILVSVENNDY"
BASE COUNT         1040 a          900 c          594 g         1572 t
ORIGIN      
        1 cggccgtcat caagtatttg aagcaagata ttaaatacat ccggatgtgc tttttcaatt
       61 tcatcaaaca aaattaccga atatggtttt cttcttactc tttcggttaa ttgacctccg
      121 tcatcaaagc ctacatatcc gggaggagag cctatcattt ttgataccga atgcttttcc
      181 atatattcgg acatgtcaat tcttatcatt gcatcttcat caccaaacat ggcttctgcc
      241 aaggctttcg agagctctgt ttttccaact cccgtaggtc ctaagaatat aaatgaaccg
      301 attggttttt ttgggtcttt aagtcctacc ctcgagcgtc tgattgcttt tgacaatgct
      361 tcaacagccg gctcctgacc tattactctt ttatgaagaa tttcctccat attcaggagt
      421 ctttcggatt catctccctg aagtttttta acggggatac ctgtccattg ggatacaacg
      481 tcggcaatat cctcataatt gatttttata tcctttatat tttttgcagc ccaaagtttt
      541 ctctgtttat taagttcatc tgtcagcttc ttttcttcat ctctcagtga cgctgccttt
      601 tcaaaatcct gattgtttat tgcagttctt ttttcagtgt caacttcttt aattttatct
      661 tccaattctt ttaattcctt gggagctgtc gatgatttta atcttatcct tgaagctgct
      721 tcatctatta agtctattgc cttgtcaggc aggaatctgt cggaaatata tctgtgagaa
      781 agacttgccg ccgcttcaat tgcttcatca gtgattgcca ccttgtgatg agcttcgtat
      841 ttatctctta atcccttaag aattcttatt gtgtcttcaa ctgtcggctc ctcaacaatt
      901 ataggttgga atctcctttc aagagcagcg tctttttcaa tatgtttttt atattcatct
      961 aaggtagttg cacctacaac ctgaagttcc cctctcgcca atacgggttt taaaatattg
     1021 gatgcatcta ttgccccttc ggcagctccg gcaccgatta ttgtatgcat ttcatctata
     1081 aataatataa tatttcctgc tgcctttatt tcatcgatgg cattttttag tctttcctca
     1141 aattctcctc tatattttgc tcctgccacc atccctgcca tatccaaggt gattacacgc
     1201 ttatccttca gtgtttcagg aacgcttcct ttgataattt cctgagccag accttctgca
     1261 attgccgtct ttccgacccc gggctcacca ataaggcagg ggttgttttt agttcttctc
     1321 gataagattt gaattaccct ttcaataaca tcttctcttc cgataactgg gtcaagcctt
     1381 ccttctttcg cacgctgatt taaatcagtg ccgtatttat caatagtctt agtatttgat
     1441 gtttgcggtg ccatactgcc gcctatatcc tgattgccgg gctccgctcc gcctgcgcct
     1501 tgcattgcca tcagcacttc attttttaaa tcctttaaac ttatcactga ttttataatt
     1561 tgtgctccta taccttcgcc ttcctcaata agtcccaata aaatatgctc acaacctaca
     1621 taaggcattt taagctgggc tgcgtactga cgtgccagct ctattataaa tttgcttctg
     1681 gggctaatag taatgtattc aggtacagaa tcacccatgc ctgttgaatt tttaattatt
     1741 tctctaactt tatcaatatc taatttatct ctaagaattt ttcctgcaat actttcctct
     1801 tcaatcatta accctaatag aatatgctcg gttccaagta cattttgttt gaaatttttt
     1861 acttcttcct gagcaagggc taaggcattt cttgcagaat tatttagtcc tcccatcata
     1921 tctttcatct cctttttgta ttatatattt caacgcaata attcacgtct tattatttct
     1981 gctctttttt tgtctctatc ctttggtgaa agttcagtat caatagttga aatgttgttt
     2041 ggctgaatcc aaatcataag ctgatatata ttcttgcctt taaccattgg agttattccc
     2101 atttcgcttc caagctttat gagcgacaat aatttcattg cctcatccac tgtcattttt
     2161 cttgcatttg taagaagtcc gtatgccctg taaaaatcat cttccaaatc gtcataattg
     2221 ttcttcatca ggtgctctct tgtgtttttt tccttatcga taatttgcat tacaatttga
     2281 ttgattttat caattaatgt ttcttcggaa gcacccaatg ttccctggtt tgaaatttga
     2341 tacaaatgtc ctattgattt tgttccttct ccataaactc ctctcacggc aactcctaac
     2401 tgggaaaccc cgtacagaag tttgtcaatt tgatttgtta ttgacagtgc aggcaaatgc
     2461 agcattacag atgctctcat acctgttcct atgttagtgg ggcaggcagt tatatatcct
     2521 aattccctgt caaatgcata atcaacactt tcctcaataa tatcatccac ttcattgctt
     2581 aatttccagc attcctttaa agataatccg tcttctatag tttggattct tatgtggtcc
     2641 tcctcattta tcataactga agttttccca tcggggctta aaagaaatgc accttcattc
     2701 cttttatttg ccaatgccgg agagataata tggttttcga tcaatgaatt tttctttaca
     2761 tctgataagt ctttcatata tgttaatttg taattttcat gtttgcttat tgcattatat
     2821 atttcattaa ttacactgtt tgattctgac tcggtcatat atatagggaa tttgtaattt
     2881 tttaaatttc ttgcaagtct gattctgctg gttattacga tatcattatt tttcaaactg
     2941 ctcatccccc tttttctttt ctaattcgta gattttatct ctaagttccg ccgcaagttc
     3001 atattcctct ctctcaacgg cacatcttaa ctcatttttc aatctttcaa tatccatttg
     3061 aaccttgtaa gctccgcctg ttcgtttagg aattttccct gtatgtctgt catatccctg
     3121 tatacttttt ataacaggtt tcaacctgcc cttgaatgaa tcaatgcatt ggctgcatcc
     3181 gaattttccc gtagttctga agttttcgta tgtcatgccg cagtttgagc atccctttgc
     3241 aggtaagtat gttttattat taaaagaatt gttcagcatt gcagagaaca aattctgcat
     3301 tgaaaaactc ggattaaaaa ttgtctggtt cttttttgca cattcttcac acaaatggct
     3361 ttctgtcttt ttcccgttaa ttatgtgagt taaatgaaca ttggcttcat ttttaccgca
     3421 ctcgttacaa agcatttata cccctcctat aaatcaataa tcattattct ctacggatac
     3481 caatatcatt tcctttaaaa ggtcggcccg cagcttattc ctgtcttcat aagcaacaat
     3541 attaagaacc ctgtcactta atacactttg cataattgct ctttcacctt ccgttattac
     3601 ttctctttcc atcagtgcat tgattaaatc aattgattta tttaatgtta tgctgtctcc
     3661 tatagtttca tttaatatgc gcttgagttc atcctccata gaagagcgaa ccctaaaaat
     3721 tcgtatatat cctcctccgc ctcttctgct ttctacaaca tatcccctgt cattggtaaa
     3781 gcgagtagta agaacgtaat taatctgaga tggagaacaa tcaaactgct gtgccaaaat
     3841 atttctttgt atttcgatta catcattatt gtttgcatcc aataattctt ttataaagct
     3901 ttcaattata tcacttaaat ttgacattcc gcacctcctt actcttctat agtttgtctt
     3961 taactgtctt tgactttgac tatctttgac cttaaactta ttttaacact attaaaaata
     4021 aaatgcaagg ggttttttga aaaaaataac aattaaaacg tagtctacag taacaaagca
     4081 tattatagga cataaaatac tgtaag
//
LOCUS       DAOPUE010000150         4083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_22100_length_4083_cov_8.680487, whole genome shotgun sequence.
ACCESSION   DAOPUE010000150 DAOPUE010000000
VERSION     DAOPUE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22100_length_4083_cov_8.680487"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKH42_09455"
     CDS             <1..293
                     /locus_tag="PKH42_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOA20701.1"
                     /translation="DRFTVYLGDVTEDKALQEKIGYNKYDMIAVNIIAQVIMSMSYTF
                     PKFLKKGGLVIASGIIKKYLQEVTDNFESLGFEIIEINESEEWVSITAKLSS"
     gene            370..1107
                     /locus_tag="PKH42_09460"
     CDS             370..1107
                     /locus_tag="PKH42_09460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOA20702.1"
                     /translation="MFRYFCADENIKNNTVNVVGGDAKHLKTILRAQPSDKISVVTES
                     KEYIAEIKEINTENIVCTLVEEIFSNNETKINITLCQGIPKKNKMETIIQQNVELGIK
                     SFIPLITERTVVKLNEKDREQKKLDRWQKIAKESAKQSKRNIIPTVEPIITVSELIEK
                     LKHIDAEIIVPYELEDVKILKDVLKEPKHNYYIVIGPEGGFDIKEIEMFQEIGAHIVT
                     LGKRILRTETAGIVTASVIMYACNEMV"
     gene            1358..2638
                     /locus_tag="PKH42_09465"
     CDS             1358..2638
                     /locus_tag="PKH42_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOA20703.1"
                     /translation="MAKKGMGLAQKVLIGLAIGFVVGLIVHYMPKGTLRDDVLIDGVF
                     QFVGQIFLRGIMMCVVPLVFVSLVNGAASMGDVKKLGKVGVKTMAFYLVTTAVAIVIG
                     LVLAFVISPGIGLDMGAIDTVDVTASEGKPIAQVLYEMVPRNPIQALAEGNMLQIIVF
                     AIITGIALTMIGEKGKKVLELFEELNELIMKLVSIVMVLAPFGVLCLVARTFGQVGVA
                     AVFPLAKSVAAVYIALALHLVLVYGGMLKLLSGLAPVRFFKKIFPAMAVAFSTASSSA
                     TLPVTMDLTVNKIGASKDISAFALPLGATVNMDGTAIYQGVSAVFIAQVYGINVTPAM
                     ALMIVLSATLASIGTAGVPGAGAIMLSMVLQTVGLPLEGLGLILGIDRLFDMGRTAMN
                     ITGDAVCTTIISKQEGEFNEEIWNTDFAKEETAK"
     gene            2876..4003
                     /gene="hemW"
                     /locus_tag="PKH42_09470"
     CDS             2876..4003
                     /gene="hemW"
                     /locus_tag="PKH42_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOA20704.1"
                     /translation="MKKLGIYIHIPFCVKKCGYCDFYSVKWDEESENKYIHSAINEIK
                     SYNELSSKYVVDSIYIGGGTPSIINPKNLEKIISTIRCLFTVEENSEISMEANPNSLW
                     ENIKTYGEIGINRLSIGIQSLNDNILKRIGRIHNSKEALQAIDRAISFGFENINADVM
                     FNIPGQTVDDINDTISKLVTKQIRHISFYSLKLEEGTPMYLLKKNKKIVMPEEDLERE
                     MYYAGRNIMEKHGLMQYEISNFSVKGYECRHNLKYWKQEEYIGIGPSAHSFLGNVRFS
                     NPSDLTEYITSGENGVFERNTLEEMDENDIKFEYIMLRLRLTEGLKFADFKNKFSIDF
                     KEAYKEQIKHLTENNLIESDDDAIRLTKRGMDLSNYVFSQF"
BASE COUNT         1484 a          575 c          843 g         1181 t
ORIGIN      
        1 aagacagatt cacggtgtac ttgggtgatg taaccgagga caaggccttg caagaaaaaa
       61 tcggatacaa taagtatgac atgattgcag ttaatataat tgctcaagta attatgagca
      121 tgagttatac tttcccgaaa tttttaaaaa agggcggttt agttatagct tcaggaataa
      181 ttaaaaaata tttgcaggaa gttacagata attttgaaag cttgggattt gaaataattg
      241 aaatcaatga aagcgaagaa tgggtatcca taaccgctaa attgtcatcc tgagaaataa
      301 ttataaaact gtcatcctga gcgtaagcga aggatctcat cagggaagtg taatattaag
      361 gaggaagaaa tgttcagata tttttgtgcg gatgaaaata taaaaaataa tacggttaat
      421 gttgtaggcg gcgatgcaaa gcatttaaaa actattcttc gggcacagcc gagtgataaa
      481 atttcagttg tgactgaaag caaggaatac attgctgaaa taaaagaaat aaatacggaa
      541 aatatagtct gcaccttggt tgaagagata ttttccaata atgaaacaaa aataaacata
      601 accctttgtc agggaatacc taaaaaaaat aaaatggaaa caatcataca gcaaaatgta
      661 gagttgggta ttaaaagctt tattccttta ataacagaaa gaaccgttgt taaacttaat
      721 gaaaaggaca gagaacaaaa gaaactggac agatggcaaa agatagccaa agaatcagct
      781 aaacagagca aaagaaatat cattcccaca gtcgaaccga taattactgt cagtgagctt
      841 attgaaaaac ttaagcatat agatgcggaa ataattgtac cttatgaatt agaggatgta
      901 aaaatattaa aggatgtttt aaaagagccg aagcataatt attacatagt aataggccct
      961 gagggaggtt ttgatattaa agaaattgaa atgtttcagg aaatcggcgc tcatatcgta
     1021 accttaggca aaagaattct aaggactgaa actgccggta tcgtaacagc ttctgtcata
     1081 atgtatgcct gtaacgagat ggtatagcgg tataaaagtt ctccggaaag ttcatcggaa
     1141 ttttccgggt ttttttattt tacaaaagaa acaaaaggaa taaaacatac tattatctaa
     1201 tacgtttatt ataaaaggag gttttgtata aattgtaaat atttgttgga caaaattcat
     1261 acgatttgat tgacaaatta tacgggaata tatacaatgt aaacgtaaac agttgattgt
     1321 ttataaagtt ttttaaattt ttaaaaggag gattataatg gcaaagaaag gtatgggatt
     1381 agcacagaaa gtccttatag ggttagccat tggttttgtc gtaggtctta tagttcatta
     1441 tatgccaaaa ggcacactta gggatgatgt tttaattgac ggtgtattcc aatttgttgg
     1501 gcagatattc ctaagaggaa taatgatgtg cgtagttcct ttggtatttg tttccttagt
     1561 caatggtgct gccagcatgg gtgatgtcaa aaaattagga aaagttggcg ttaaaacaat
     1621 ggcattttac ttagtgacta cagcagtagc aatagtaata ggtcttgtat tggcattcgt
     1681 aataagtccg ggtatcggat tagatatggg tgctatagat actgttgatg ttactgcaag
     1741 cgaaggtaaa ccaatagcac aagttcttta tgaaatggtg ccgagaaacc cgattcaggc
     1801 attagctgaa ggaaatatgc ttcaaattat tgtatttgca ataataaccg gtatagcatt
     1861 gaccatgatt ggtgaaaagg gtaaaaaagt acttgaatta tttgaagaat taaatgaatt
     1921 aataatgaaa ttagtaagta ttgtaatggt tttagctcca ttcggagtgt tatgtctggt
     1981 agccagaacc tttgggcaag taggagttgc cgctgtcttc ccgttggcaa aatctgttgc
     2041 tgcggtttat attgcattag cattacattt agtacttgta tatggcggaa tgctaaaatt
     2101 gttatcggga ttggcaccgg ttagattctt taagaaaatt ttccctgcaa tggcagttgc
     2161 attctcaaca gccagcagca gtgctacatt accggtaaca atggatttga ctgttaataa
     2221 aataggtgct tctaaggata tatcagcgtt tgctcttccg ctcggtgcaa ccgttaatat
     2281 ggacggaact gctatatatc aaggagtttc agctgtattt attgcacagg tatatggaat
     2341 taatgtaaca cctgctatgg cacttatgat tgtgctatca gctacattgg catcaatcgg
     2401 tactgcagga gttccgggtg ccggagcaat aatgctttcc atggtattgc agacagtagg
     2461 attgcctctt gaaggattag gtttaatact gggtattgac agactattcg acatgggtag
     2521 aactgccatg aacattacag gtgatgctgt atgtactaca ataatctcta aacaagaagg
     2581 agaatttaac gaagaaattt ggaatactga tttcgcaaaa gaagaaacag ctaaatagtc
     2641 agactatgtg ggacagtggg attgaaccga ctgtcccttt tttttgtcat cctgagcgaa
     2701 gcgaaggatc tcatgagatt cttcgagcta aagctctcag aatgacacaa aggcaagtca
     2761 tgataaaatg gtaaaacctc agaattatca cgtgttaata caccacgata aacgatatgt
     2821 tctgtgattg tcatcctgag cgaagcgaag gatctcataa aaaacagagg tagttatgaa
     2881 gaaactagga atttatatac atattccatt ttgcgtaaaa aagtgcggtt actgcgactt
     2941 ttattcggta aaatgggatg aagaatcaga aaataaatat attcattcag caattaacga
     3001 aataaaaagc tacaatgaat tgtcaagtaa atatgtggtt gacagtatat atatcggagg
     3061 aggaactcct tcaattataa atccgaaaaa tcttgaaaaa atcataagca ccataagatg
     3121 tttatttacc gtagaagaaa attccgaaat aagcatggaa gcaaacccta actcattatg
     3181 ggaaaatata aaaacttacg gagaaatcgg gattaacaga ttgagtatcg gaattcaatc
     3241 attaaatgat aatatattaa aaagaatagg caggattcac aattcaaaag aagctctgca
     3301 agcaattgac agagctatta gttttggatt tgagaatatt aatgcagatg ttatgtttaa
     3361 cattcccgga cagaccgtcg atgatataaa tgataccatt tctaaattag taacaaaaca
     3421 aatcaggcat atatcctttt attcactgaa attagaagaa ggaacaccaa tgtatttgtt
     3481 gaaaaaaaac aaaaaaattg ttatgcctga agaagacctt gaaagggaaa tgtattatgc
     3541 aggcagaaat attatggaaa agcatggttt aatgcagtac gaaatttcaa atttttccgt
     3601 gaaaggatat gagtgcaggc ataatttgaa gtattggaaa caagaagagt atataggaat
     3661 cggaccttcg gctcattcat ttttaggaaa cgtaagattc agcaatcctt cagacctcac
     3721 agaatatata acaagcggtg aaaacggagt ttttgaaaga aacactcttg aagaaatgga
     3781 tgaaaatgat attaaatttg aatatataat gctgcgttta aggctaacgg agggacttaa
     3841 atttgcagat tttaaaaata aattttcaat tgattttaaa gaagcatata aagagcaaat
     3901 aaaacatctt acagaaaaca acttaatcga atcggatgat gatgccattc gccttactaa
     3961 aaggggaatg gacctatcga attatgtatt ttctcagttt taatacactt attgtcatcc
     4021 tgagcgatag cgaaggatct catgagattc ttcgagctaa agctctcaga atgacacaaa
     4081 ggc
//
LOCUS       DAOPUE010000151         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_22988_length_3968_cov_10.080756, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000151 DAOPUE010000000
VERSION     DAOPUE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22988_length_3968_cov_10.080756"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKH42_09475"
     CDS             complement(<1..397)
                     /locus_tag="PKH42_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOA20705.1"
                     /translation="MIIDVIILIFTGYLFFQGYRKGFILTVFDTLGMIISFFLSKRFY
                     YIGEDFLLNNTKLFVKLHDYFEVKLSPSAFNNLSKIPVELQKFVNNIMASETSDTFAI
                     FVDNLSLLIIRSISFIVTFLSIYAILLILS"
     gene            complement(417..1472)
                     /locus_tag="PKH42_09480"
     CDS             complement(417..1472)
                     /locus_tag="PKH42_09480"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HOA20706.1"
                     /translation="MKKLILLIAIILIFIVAFLYSPYLKNILEALDENKVYKIENIKT
                     TEINTLEEFKFFGRGIVSYNNQKIVYTDFNNKILWENQNKEFSNKVFVTDKYIFRQTN
                     NNIMLLDNNNQEFIIAEIEGNIINVSRENGKTAIIVKGNGQSLYILNENNEVVVDKKE
                     FQDIITGISISDKSEAYSLITLTFDNGNPVNTLYYNLIDDVELWNSSISNEILLKIKV
                     VNNNVIAVGTENIYFYNNNGKLMWKNSIYNRILDYEILQDRINILFEKGESTELISYN
                     FEGKVIEIQAAPFGVHKLKIVDNKTLVYNNNSIYLLHSNKSDKIFEEFESFEDFLIEG
                     NTIYILFKNKIMSGQIK"
     assembly_gap    1517..1616
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1617..2569)
                     /locus_tag="PKH42_09485"
     CDS             complement(<1617..2569)
                     /locus_tag="PKH42_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOA20707.1"
                     /translation="MIYLDNAATTFPKPSSVYKSVMRAMTVYGANPGRGSHAMAIEGA
                     RIIYETRELLAQLFNLDDPMKVIFTFNATDSLNIAIKGILNSGDHVVTTEMEHNSVLR
                     PIKELEKSGVENTIVKCSPDGTINLNDLESAIKENTKLIVTTHVSNLTGTIFPIEEIG
                     KICKKHNILYLLDASQSAGVLNIDLKKYNIDFLAAPGHKGLLGPQGTGILLINNETEM
                     KQLKEGGTGSRSSSLEHPDFYPDKLEAGTHNLPGIAGLNAGIKYILNRGIESIYSHEK
                     KLLEIFIAEIRKNPKIKIYGPECIELRSGVVPVNIDGMDSS"
     gene            complement(2629..3885)
                     /locus_tag="PKH42_09490"
     CDS             complement(2629..3885)
                     /locus_tag="PKH42_09490"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOA20708.1"
                     /translation="MIIKNGRVIDPQSKMDEIADILIEEGIITRIHKNINIKDEVINA
                     EGKIIAPGLIDVHVHFREPGFEYKEDILSGSKAAARGGFTTVVCMANTSPAVDNIETL
                     KYINDIIQHSPINILQTAAVTKGLKGTEIVNMEELKKYGAVGFSDDGFPITDTNIVLN
                     AMLEAKRLSLPISFHEEDPCFIDSPGINEGKISEKLGIKGASHLAEDVMVARDCIIAL
                     ETGAKINFQHISSGLSVDIIRWAKSMGANISAEASPHHFSMTEDDILQYNTNAKMNPP
                     LRTENDRIKLIEGLKDGTIDIIATDHAPHSNEEKNREFIKAPSGIIGLETALSVGITY
                     LVRNKHLTLMQLLEKLTVNPAKLYNLQSGIKEGNKCDLVIFDMDEMWIADEFYSKSRN
                     SPFIGRELCGKVKYTICNGKVVYKDK"
BASE COUNT         1149 a          717 c          511 g         1491 t
ORIGIN      
        1 atgatagaat aagcagtata gcatatattg ataagaaggt tacaataaag cttatcgacc
       61 ttattattaa aagacttaaa ttatctacaa aaatagcaaa ggtatccgac gtttcgcttg
      121 ccattatgtt atttacaaac ttctgcagct caacgggaat tttactaaga ttattaaatg
      181 cgcttggtga aagtttcact tcaaaataat catgcagttt tacaaaaagc tttgtattgt
      241 ttagtaaaaa atcttcacca atataataaa accttttgct taagaaaaaa gaaattatca
      301 tgcctaatgt atcaaagacc gttaatatga atcccttcct gtacccttgg aaaaacaagt
      361 atcctgtaaa tattagtata ataacatcaa ttatcacgct agttccccct tttaatttat
      421 tttatttgtc ctgacataat cttgttttta aatagaatat aaatggtatt tccttcaatc
      481 aagaaatctt caaaactttc aaattcctcg aatattttat cgcttttatt actgtgaagc
      541 aaataaatag aattattatt ataaaccaaa gttttattat caactatttt caacttgtgg
      601 acaccgaaag gggctgcttg tatttcaata accttacctt caaagttata agatattaat
      661 tctgtagatt cgcccttttc aaacaatata ttaattctat cctgtaatat ttcataatcg
      721 agtattctat tatatatact gttcttccac attaattttc cgttattatt ataaaaatat
      781 atattttctg ttccaacagc tattacattg ttatttacta cttttatttt aagaagaatt
      841 tcatttgata ttgatgaatt ccacaattcc acatcatcta taagattata atataatgta
      901 ttgacgggat ttccattgtc aaaagtcaaa gttataagtg aatatgcttc ggatttatca
      961 ctgatggata taccggttat tatatcttgg aattctttct tgtccacaac tacttcatta
     1021 ttttcgttca atatatacag ggattgtcca tttcctttta ctattattgc cgtctttccg
     1081 ttttcacgtg aaacatttat aatattccct tcaatttcag ctattataaa ctcctgatta
     1141 ttattatcca gcaacattat attgttattt gtttgcctga aaatatactt gtctgtaaca
     1201 aatactttgt tagagaattc cttgttttga ttttcccata aaattttatt attgaaatcc
     1261 gtataaacta ttttttgatt attatatgat actatacccc tgccaaaaaa cttaaactct
     1321 tcaagcgtat taatttctgt tgttttaata ttttcaatct tatatacctt attttcatcc
     1381 agtgcttcca aaatattttt taaataagga gaataaagaa aggcaactat aaaaattaga
     1441 attattgcaa ttaataatat taactttttc atatatcacc ctgtaaaatt ttgttaatca
     1501 attataccaa acttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcgg
     1621 aagaatccat tccatcaatg ttaacaggaa caacaccgct tcttagttca atgcattccg
     1681 gaccgtatat tttgatttta gggtttttcc ttatttctgc aatgaatatt tcaagaagtt
     1741 ttttttcgtg gctgtaaatc gattctatgc ctctgtttaa aatgtattta attccggcat
     1801 ttaatcctgc aataccgggt aagttatgtg tgcctgcttc cagtttatca ggataaaaat
     1861 ccggatgctc taaactgctt gaccggctgc cggtgccgcc ttcttttaat tgtttcattt
     1921 ctgtttcgtt atttatcaaa agaattcccg tcccttgagg acctaacaat cccttatgac
     1981 cgggcgctgc cagaaaatca atattgtatt tttttaaatc tatatttaaa actcctgcgc
     2041 tttgtgaagc atccaataga tacagtatat tatgtttttt acatattttt ccaatttctt
     2101 caatgggaaa aatggtacct gttagatttg aaacatgagt tgtaacgatt aatttagtat
     2161 tttcttttat tgcactttcc aaatcgttta aatttattgt tccgtccgga gaacatttta
     2221 caattgtatt ttctactcct gatttttcca attccttaat aggtcttaaa acagagttat
     2281 gctccatttc agtagtaaca acatggtctc ccgaatttaa aatacccttt attgcaatgt
     2341 ttaaactgtc tgtcgcatta aaagtaaata ttactttcat cgggtcatct aaattaaaaa
     2401 gctgggctaa gagctctctt gtttcataaa taattctggc accttcaatt gccattgcat
     2461 ggcttcctct gccgggattt gctccgtaga cggtcattgc tctcattacg cttttatata
     2521 ctgatgaagg cttaggaaat gttgtagctg cattatctaa atatatcata atatcacctg
     2581 ctgtttttat tttcaatttc attatatgta aattacagcg tttggatatt atttatcttt
     2641 gtaaacaacc ttcccgttac aaatggtgta tttaacctta ccgcataatt ctctgcctat
     2701 aaatggagaa ttccttgatt tagaataaaa ttcatcggca atccacattt cgtccatgtc
     2761 aaatattacc aaatcacact tattgccctc tttaattcct gactgcaaat tatacagttt
     2821 tgcagggttt acagttaatt tttctaaaag ttgcattaat gtaagatgct tatttcttac
     2881 taaatatgta ataccaacgg ataatgcggt ttccaaaccg attattccgc tgggagcctt
     2941 gatgaattct ctattctttt cttcattgct gtgtggagca tgatccgtag caattatatc
     3001 tattgtaccg tctttcaagc cctcgattaa ttttattctg tcattttccg ttctcaatgg
     3061 gggattcatt tttgcatttg tattatattg tagtatatca tcttccgtca tggaaaaatg
     3121 atgaggtgaa gcttccgcac taatatttgc ccccattgac ttagcccacc taattatgtc
     3181 aaccgataaa cctgaactta tatgttgaaa gttaattttt gcacctgttt ccaatgctat
     3241 aatacaatct cttgcaacca ttacatcttc agcaagatga gatgctccct taataccaag
     3301 cttttcggat attttgcctt catttattcc cggactgtct ataaagcaag ggtcctcttc
     3361 atgaaagctt ataggtaaac ttaatctttt cgcttccaac attgcattta atacaatatt
     3421 tgtatccgtt attggaaatc cgtcatcaga aaatcctact gccccgtact tttttaattc
     3481 ttccatgttt acaatttcgg taccctttaa tcccttagtt actgcggcag tttgcaatat
     3541 atttataggt gaatgttgta ttatatcatt tatatatttg agagtttcga tattatctac
     3601 tgctggagaa gtgttagcca tacatactac cgttgtaaaa ccgcctcttg cagctgcttt
     3661 tgagcctgat aatatatctt ccttatattc aaatcccggc tccctgaaat gcacatgcac
     3721 atctattaat cccggagcta ttattttacc ttcagcattt ataacttcgt cttttatatt
     3781 aatattctta tgtatccttg tgataatgcc ttcctcaatc aaaatgtcgg caatttcgtc
     3841 catttttgat tgcgggtcta ttactcttcc gttttttata atcatctatc tcctgcttcc
     3901 tttactctta ttaatttatt atatcataag tgcaagccgc tataggtctg cctcacatcc
     3961 caatgaaa
//
LOCUS       DAOPUE010000152         3960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_23053_length_3960_cov_9.662484, whole genome shotgun sequence.
ACCESSION   DAOPUE010000152 DAOPUE010000000
VERSION     DAOPUE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23053_length_3960_cov_9.662484"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..194
                     /locus_tag="PKH42_09495"
     CDS             57..194
                     /locus_tag="PKH42_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20709.1"
                     /translation="MIRRKTARANAYVDFAIEVTKKLNLFQDEADLAETVVFWKEHKR
                     I"
     gene            216..1388
                     /locus_tag="PKH42_09500"
     CDS             216..1388
                     /locus_tag="PKH42_09500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOA20710.1"
                     /translation="MELLTKIARPRPAGTIQNMEITDYIGSYLEKLGYVMRKVPFKCK
                     VWESQDSYLVIGGNKLTLHVNPFSEPFNGIRRAIFVRNLEELERVECEDKILFLTEYL
                     AKEPLQPKNYPFYYPDEHKIIIDCLEAKKPCAIIALTGGTSMSGLKPFPLIEDGNFHI
                     PVASLDKDIFPGIEDKVTEEDIELSIVSKNNIVTAHQLIASKEVSDSKGTIVICAHMD
                     SKYNTNGALDNASGVAVMLLAAKGIETNRYNIDIVPFNSEEYFDPQGELIYLEDLRKS
                     EKEISLLINIDTVAHVGSKVAVAYFNFNDKEQLELDNIMNTCTNIVSGQPWYAGDHAA
                     FVFGGTRCILISASDLFEGCLSYTHCPKDTIDLVDEKLIENVAEYICKVVNDITLR"
     gene            1488..1943
                     /locus_tag="PKH42_09505"
     CDS             1488..1943
                     /locus_tag="PKH42_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOA20711.1"
                     /translation="MNKEKEALIAPCGMNCSLCIAYQFKEKDLNKRGFHKKYCPGCLP
                     RGKNCLHMGDVCELLGKGKIRFCFECEDYPCKRLKALDKRYRTKYHMSMIENLNYIRD
                     NGLKNFLENEEIKWRCTGCGGKICCHNGLCLNCNIDVLVQNRRYRWNEE"
     gene            2013..2459
                     /locus_tag="PKH42_09510"
     CDS             2013..2459
                     /locus_tag="PKH42_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HOA20712.1"
                     /translation="MDNIHELVYGLRDKDNDYAYQCLKQLQAESSNSDKVYSYFDYFT
                     EMLDDSSSYIRARGIHMIAANAQWDKDCKLDGIIDKFLEHILDEKPITARQCIQLLPI
                     IAEHKPNLKEKIVSALLKAEPKKYKETMQKLIFKDIEKSLELIANK"
     gene            complement(2522..3112)
                     /locus_tag="PKH42_09515"
     CDS             complement(2522..3112)
                     /locus_tag="PKH42_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20713.1"
                     /translation="MKKHNHYETLFRKNKNTGRVIIDIALGDYIEFFNEWDNSAFKMR
                     DIHSDLVRFLDLCSDYIPLRKKIEIVFSIEASEADKEKEDSIRLSYGNYYNALKRLES
                     RKANRYIRMSIILLIISLILLTSYGFFYEIQVETIGSLVLLESLLIGGWVFAWEAVHT
                     LFIDIIEPVRRIREIQRFIEADITFVHTGSNNKESV"
     gene            3303..3797
                     /gene="ybaK"
                     /locus_tag="PKH42_09520"
     CDS             3303..3797
                     /gene="ybaK"
                     /locus_tag="PKH42_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOA20714.1"
                     /translation="MGKSEKKQGEQKTNVMRLLEQAKIPYRHHCYVDTAAISGIEVAS
                     VLGQDENRVFKTLVTVGKSGEHYVFVVPVNRELNLRKAAKAVNEKSVEMIKTKELLPL
                     TGYIHGGCSPIGMKKRFTTVVDESAVGQDTIFVSAGKIGYQIEIKPDDLKKIVQFYYD
                     DITE"
BASE COUNT         1405 a          607 c          812 g         1136 t
ORIGIN      
        1 cgggaaaaga atatggatga ctggattata gtagaaagta ttgacgatat caggatatga
       61 taagaaggaa aactgccaga gccaatgcat atgtggattt tgccattgaa gtcaccaaga
      121 aactaaatct ttttcaggac gaggctgatt tggctgagac ggtggtattt tggaaagagc
      181 ataaaaggat ataataaaga atggagaagt cagaaatgga attattaaca aaaattgcaa
      241 gaccaaggcc ggcaggaact attcaaaaca tggagattac agactatatt gggtcatatc
      301 tggaaaaatt aggatatgta atgcgcaagg taccctttaa gtgtaaggtg tgggaatcac
      361 aagattcata tttagtgatt ggtggaaaca agttgacatt gcatgtaaac cctttttctg
      421 agccattcaa cgggataaga agagccatct ttgtcagaaa tttggaagaa cttgaaagag
      481 tagaatgtga agacaaaata ttgtttttaa ctgagtattt ggccaaagag ccattgcaac
      541 cgaaaaatta tcccttctat tatccggatg aacataaaat aattattgat tgcctggaag
      601 caaaaaaacc ttgtgcaatt atagccctta caggcggaac aagtatgagc ggccttaagc
      661 cctttccgtt gattgaggat ggaaattttc atattcctgt agctagtctt gataaagata
      721 tttttccagg aatagaagat aaagtaacag aagaagatat tgagctatct atagtttcaa
      781 aaaataatat tgtaacagca catcagctga ttgcttcgaa ggaggtctct gactctaaag
      841 gtacaatagt gatctgtgcc cacatggaca gcaagtacaa tacaaatggt gcccttgata
      901 atgcatccgg agttgcggta atgcttttgg cagctaaggg tattgaaacc aacagatata
      961 acattgatat cgtacctttt aattctgaag aatattttga cccgcaggga gaactaatct
     1021 atttggagga cctaagaaaa agtgaaaaag aaatatcgct tctgataaat atagacacgg
     1081 tagcacatgt cggatctaaa gtggcagtgg catattttaa ttttaatgac aaagaacagt
     1141 tggaattaga taacataatg aatacttgta ctaacatagt tagtggtcag ccctggtacg
     1201 ctggagacca tgctgctttt gtttttggtg ggacaagatg tatattaatt tctgcatcag
     1261 atttatttga aggatgttta tcttatacac attgtccgaa agatacaatc gacctggttg
     1321 atgagaagtt aattgaaaac gtagcagagt atatatgtaa agtagtaaac gatatcacac
     1381 taaggtaatg ggggtaatta aaagctggcg cttaactgtt gttgcccaag tctgagcctt
     1441 atattattaa ttttaaatga aaggggtatt gctttgaagg tgatgaaatg aataaagaaa
     1501 aagaagctct tattgctccg tgtggtatga attgcagttt atgcattgct tatcaattca
     1561 aagaaaagga cttaaataaa agaggtttcc acaagaaata ttgtccgggc tgccttccca
     1621 gggggaaaaa ctgcctccat atgggagatg tttgcgagct tcttggaaag ggaaagatcc
     1681 ggttctgttt tgagtgtgaa gattatccgt gcaagagatt aaaagctctg gataaacgtt
     1741 atcgtactaa atatcatatg agcatgatag agaatttaaa ttacattaga gataatggat
     1801 taaagaactt cctcgaaaac gaagagatta agtggaggtg cacagggtgc ggaggtaaga
     1861 tctgctgcca caacggtctg tgcctaaatt gcaatataga tgtattagta caaaacagaa
     1921 gataccgatg gaatgaagaa taggtcaatg gaatcgatga attgcataat gatataaaat
     1981 acaattaaat tttaataggg agggtaatta atatggacaa tattcatgaa ttggtttacg
     2041 ggttaagaga taaagacaat gattatgcat atcagtgttt aaagcaactg caagcggaaa
     2101 gttcgaactc ggataaggta tactcctatt tcgactactt tacagagatg ctggatgatt
     2161 caagttcata catcagggct agaggaatac atatgattgc tgcaaacgct caatgggaca
     2221 aagactgcaa attagatgga atcatagata aattccttga acatatcttg gatgaaaaac
     2281 ctattacagc aagacaatgc atacagcttc ttccgataat tgcagaacac aagccgaatt
     2341 taaaggagaa aatagtcagt gctttactta aagcagaacc taaaaaatac aaagaaacca
     2401 tgcaaaaact aatatttaag gatatcgaaa aatcactgga actgatagca aacaaatgaa
     2461 gtggtaagta gggaataatt ccgcagaaca aaccctgcgg aattatcaat tagataagta
     2521 tttaaactga ttctttatta ttggaacccg tatggacaaa agtgatatct gcctcaataa
     2581 atcgctgtat ttcacggatt cttcgtaccg gctctataat atcaataaac agggtatgta
     2641 cagcctccca ggcaaaaacc catccaccga tcagtaagct ttcaagcaaa accaatgaac
     2701 caattgtttc tacttgaatt tcgtagaaaa atccatacga tgtcagcaat ataagtgata
     2761 taataagcag aattatcgac atcctgatat atctgtttgc tttacggctc tccaaacgtt
     2821 tcaaagcatt atagtaattg ccgtaactca agcggattga atcttctttt tctttatctg
     2881 cttcacttgc ttcaatagaa aaaacaattt ctatcttttt tcgcaggggg atataatctg
     2941 aacataagtc aagaaatctg accaagtctg aatgtatgtc acgcatctta aaggcggaat
     3001 tatcccattc attaaagaac tcgatataat ctcccaaagc aatatctata attacacggc
     3061 ctgtattttt attttttctg aacaaggttt cataatggtt atgttttttc atatataccc
     3121 ctgtagtttt ttaatctgct tattgaatct gttaacagat tataccatac ctctgaaggg
     3181 ttttgcatta taaatttcat acaagataat attttatata caagaagaat tttttgatta
     3241 taattaatat aataaggtcc tttgcttatg cttaggatga aatggaatca ggggtgagac
     3301 ctatgggaaa gagtgaaaag aaacaaggag agcaaaaaac caatgtaatg aggttgctgg
     3361 aacaagcaaa gataccctac cgtcaccatt gctatgtaga tacggctgcc ataagcggta
     3421 ttgaagtagc atctgtactc ggccaggatg aaaaccgggt gtttaaaacg cttgttactg
     3481 tggggaaatc cggcgaacac tatgtttttg tagttcctgt aaacagagaa ctaaatttgc
     3541 gaaaagcggc aaaagcggtg aatgagaagt ccgttgaaat gatcaaaacg aaagaattgc
     3601 tgccgcttac cggatatatc cacggcggat gttcaccgat tggcatgaaa aaaaggttta
     3661 ctacggttgt ggacgaaagt gctgtaggtc aggatacaat ttttgtcagt gccggaaaga
     3721 taggatatca gattgagata aaaccggatg acctgaaaaa aatcgttcag ttttactatg
     3781 atgacataac agaataataa aagatatatg aaatctatat tttatgttat actatttaag
     3841 ataagctctt ttgcatgatt tttattaaaa attaataatg cacagttagt aattttctaa
     3901 aaaggacaaa ttatgatcac attaaacata gccgaaacac tactatataa tatcggtatt
//
LOCUS       DAOPUE010000153         3946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_23179_length_3946_cov_8.182215, whole genome shotgun sequence.
ACCESSION   DAOPUE010000153 DAOPUE010000000
VERSION     DAOPUE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23179_length_3946_cov_8.182215"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1417
                     /locus_tag="PKH42_09525"
     CDS             377..1417
                     /locus_tag="PKH42_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOA20715.1"
                     /translation="MNKKKLFAIVVCIIIALVATYIGGAQKIIGAPMLGLLIGMAVVN
                     MIPSVDKDFKSGTSFAGKKFLNLGIILTGATLNFNQILGYGARAMPLLLFNICVAFLV
                     SFTIGKKFRVSANTSTLVGSGSCICGGTAIATMSSVIGAKETEIAYAMTAIFLFDILS
                     ALLYPYLATALNLTQNQFGFLAGTSINDTSSVVAAESTYNVLNNIDSSLAVTIKLART
                     TLLILLVVVFTVLKAKEQSVTNVAGESSKNVSIGRTVIKTFPKFILVFLLMAVLNTIG
                     VFDGISGAAVFFKKYSKFFITTALAGVGFKIQFKDLFTEGLKPIVLGGITWLSICISS
                     MLFITIFANYVG"
     gene            1650..2477
                     /locus_tag="PKH42_09530"
     CDS             1650..2477
                     /locus_tag="PKH42_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOA20716.1"
                     /translation="MKFISWNIDSLNAALTGDSPRANLSQNVLNTIKEYNPEVIALQE
                     TKLSSNGPAKKHLQVLDEMFPDYEIVWNTSVEPARKGYAGTMFLYKKDLKPTVSFPKI
                     GAPGTMDAEGRIITLEFDNFYLTQVYTPNAGDNLNRLEDRQVWDIKFADYLESLDKEK
                     YVIATGDFNVAHKEIDLAHPDNNRRSAGFTDEEREGFTNLLNRGFTDTFRYIHGDVPG
                     VYSWWAQRAVTSKINNSGWRIDYWLVSNRIADKVIKSEMIDSGPRQDHTPILLEICL"
     gene            2550..3578
                     /locus_tag="PKH42_09535"
     CDS             2550..3578
                     /locus_tag="PKH42_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOA20717.1"
                     /translation="MSKVYFIKNTESDYSKLGKDAAELLKRIVSEAGHKFEKEVPIKV
                     HFGEKGNKTFIPAVCYDDTIKYLKENDVSPFYIESNVLYRGSRTTTQKHIETAKSHGF
                     TQIPIIIADGDIGTEYNEIEINKEYIKTCKIGKAYGDYKQFIVMSHFKGHVEAGFGGA
                     IKQLGMGFASRGGKLEQHSGISPVVNEKKCISCGICIDKCNYSAITKTNVAVIDSSKC
                     IGCAGCIAVCPQGAIRNTWGGSNFFEKLAEYAYGAAKDKDIIFITFVHNITKDCDCNG
                     THMKLIADNVGILAGKDPVSLDTACLDLVQKTEGNKLFDKGRNTLKHAEKIGLGEMNY
                     ELIKIKIP"
     gene            3575..3703
                     /locus_tag="PKH42_09540"
                     /pseudo
     CDS             3575..3703
                     /locus_tag="PKH42_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
BASE COUNT         1344 a          595 c          785 g         1222 t
ORIGIN      
        1 ccagccttgt aggtgcaatg accgatacgg ttttaattct ttttccggat actgttataa
       61 gcaggacttc atttataatc ggcggactat cgggagttac aatgggcagg ctcttaatgc
      121 ctggaatcat aatatttagt tcgcttattg taacatttat attcagttag agaaaacaag
      181 aaatattaga agctgaactt catatgacag gcggagctac ctgctaaaga aaactatatc
      241 cggaagaata tttcttccgg agataatttt ctcatacttc gttaaaaaaa taattaacga
      301 accggagaaa gttaaatttt aggtatggcg tttttaaacg cttggtatta ttatgtaatt
      361 agaaggagaa aatttcatga ataagaagaa actttttgct atagttgtct gcattattat
      421 agctcttgtt gcaacatata taggtggagc tcaaaaaata atcggtgctc caatgttagg
      481 actattgatt ggtatggctg tagtaaacat gattccgtct gtagacaaag attttaaatc
      541 aggtacaagc tttgcaggaa agaaattttt gaatttaggt attatattaa ccggtgcaac
      601 acttaatttt aatcaaatat taggctatgg ggcaagagct atgcctttat tattatttaa
      661 catatgtgta gcttttttag tttcattcac tataggcaaa aaatttagag tatccgccaa
      721 tacatcaacc ctggtaggca gcggttcttg tatttgcggc ggtaccgcaa ttgcaacaat
      781 gtcatctgtt attggagcga aagaaaccga aatagcatat gcaatgacag ctatatttct
      841 gtttgatata ctttctgcgt tattatatcc atacttggca actgctttaa atttaactca
      901 aaatcaattc gggtttttag ccggtacaag cattaatgat acttcgagtg ttgtagctgc
      961 tgaatctaca tataatgttt taaataatat tgattcaagc ttagccgtta cgataaaact
     1021 tgcaagaaca acattattaa ttcttcttgt ggttgtattt accgtcttaa aagcaaaaga
     1081 gcaatctgta acaaatgttg ccggtgaatc aagcaaaaat gtgtcaatag gaagaactgt
     1141 tattaaaaca ttccctaagt ttattcttgt atttttattg atggctgttc ttaatacaat
     1201 cggtgtattt gacggaatca gcggagcagc agtattcttt aaaaaatact caaaattttt
     1261 tattaccact gctttagccg gggttggatt taagattcag tttaaagacc tatttactga
     1321 aggccttaag cccatagtat taggtggaat tacatggtta tctatatgta tatcaagtat
     1381 gttatttatt acgatatttg caaactatgt tgggtagatt aattaaaaac ttgagctttt
     1441 ttggtcaaat tccctaatgt gttgatagta aagtcccgcc ataatatttt attatggcgg
     1501 gactctttct taaacgaact ttgttgaacg aattatgcaa gcggaatggt tacggtaaaa
     1561 gagataaaga ttgcagattc ttattaaata gtttaaaata aaagaaacat aataactata
     1621 taattacaaa aaataagggg gataaggcat tgaagtttat ttcatggaat attgattcat
     1681 taaatgcagc attaacaggt gattctccac gtgctaattt atctcaaaat gtattaaata
     1741 caattaaaga atataatccg gaggttattg cccttcagga aacaaagcta tcaagcaatg
     1801 gtcctgctaa aaaacatttg caagtcctgg atgaaatgtt tcccgactat gaaatagttt
     1861 ggaatacttc ggtagaaccg gcccgcaagg gttatgcggg caccatgttt ctatataaaa
     1921 aggatttaaa accaactgtt tcctttccga aaatcggggc accgggtact atggacgctg
     1981 aaggcagaat cataacttta gaatttgaca atttttattt aacccaggtt tatactccaa
     2041 acgcggggga taatttaaat cgtttggaag accgccaggt ttgggatata aaatttgcag
     2101 actatttgga aagtttggat aaagaaaagt atgttattgc aacaggtgat tttaatgtgg
     2161 cacacaagga aatagacttg gctcatcctg acaataaccg tcgttcagcc ggttttaccg
     2221 atgaggagcg tgaaggcttt acaaacctgt tgaacagagg ttttacggac actttccgtt
     2281 atattcacgg tgatgtgccg ggtgtttatt cttggtgggc tcagcgtgcc gtaaccagta
     2341 aaattaataa ttccggttgg agaattgatt attggctggt aagcaatcgg attgcagata
     2401 aggttatcaa gtctgaaatg attgattcag gcccaagaca agaccataca cctattttgc
     2461 ttgaaatatg cttatagcaa ttctaactaa ttcccttgga ccaaagtttg agggaatgat
     2521 aaaaataata tgaaagagaa ggtgcacagg tgagtaaagt atactttatt aaaaataccg
     2581 aatctgatta tagtaaactt gggaaagatg cagcggaact attaaaaaga atagtttcag
     2641 aagcagggca taagtttgaa aaggaagtac ctattaaggt tcattttggg gaaaagggaa
     2701 acaaaacatt cattcctgcc gtatgctatg atgatactat aaaatacttg aaagaaaatg
     2761 acgtctcacc cttttatatt gaatctaacg tattatacag aggttcaaga acaactaccc
     2821 agaaacatat agagacagcg aaatcacacg gttttacgca aattccgatt ataatagcag
     2881 atggagatat aggaactgag tataatgaaa tagaaataaa caaggaatat ataaaaacat
     2941 gtaagattgg caaggcttat ggagattata agcagtttat agttatgagt cattttaagg
     3001 gtcatgtaga agcaggcttt ggaggtgcaa taaagcaact tggtatgggt tttgcctcaa
     3061 ggggaggaaa acttgagcag cattcaggca tatcccccgt cgtaaatgaa aagaaatgta
     3121 tatcctgcgg catatgtatt gataaatgca actacagtgc aataacaaaa acgaatgttg
     3181 cggttattga cagcagcaaa tgtatcggtt gtgccggttg tattgcggtt tgcccacaag
     3241 gtgccattag gaatacatgg ggcggttcaa acttctttga gaaacttgcg gagtatgcct
     3301 atggagcagc aaaggacaaa gatattatat tcattacatt tgttcataat atcactaagg
     3361 attgtgactg caacggaaca catatgaagc tgattgcaga taatgtcggt attcttgcag
     3421 gtaaagatcc cgtatcctta gataccgcat gtttagattt agtacagaaa acagagggca
     3481 ataagctttt tgataaaggc aggaatactt taaagcatgc ggaaaaaatc ggcctgggtg
     3541 aaatgaatta tgagcttatt aaaataaaga taccttaata gatgtgcata ttgtggttga
     3601 gcgccttgtt ccgaagttgg cccgtttaac tttctgtaaa aagggacaaa caagtgaatt
     3661 ggcaacaaaa cgctatcatg aaatccttag aaatcttcaa taaagctcca taaatttaaa
     3721 ttttctctgt aatctaactt tttattatga ccaataatat aacaatttat actacattct
     3781 ctccaagtga gaaatgttag tagacattta atattgttga cataagcatt ttattaataa
     3841 acttatgagg ataaactagt gcacttagtg taaaataatt gtttgacaaa gaaataaaaa
     3901 atatagagaa ttaacctcca tttgatataa tagtagttga ccaaac
//
LOCUS       DAOPUE010000154         3914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_23441_length_3914_cov_10.137082, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000154 DAOPUE010000000
VERSION     DAOPUE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23441_length_3914_cov_10.137082"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKH42_09545"
     CDS             complement(<1..190)
                     /locus_tag="PKH42_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20718.1"
                     /translation="MVKNNVKFKPIVLIFIVAIIGFFVSGIPFRVFAKLSVGDEYKNI
                     YAEYENMNQDINLKSLYEE"
     gene            complement(365..1795)
                     /locus_tag="PKH42_09550"
     CDS             complement(365..1795)
                     /locus_tag="PKH42_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20719.1"
                     /translation="MAVILSIDIDNNIKIIEASKKGETLSVLRCMTIESSGAKDGKII
                     DMDGIVNVINEEFKNNGIKTRKAIFVINSSKIMIRKIKLPLLKKSPEILSMLQIELQQ
                     IVSVDLSKYKIVYEISNATNENRIPYAEYIVYCVPVILVSECVELAAKLKLKLLKIDI
                     SHNCINSLYKNNIKINDVGLNNGENAAFISVNKNFISFSTAHNGFCDFHISSDLEEIV
                     AQLTKFMRYYYSVSGNRSIEKIYIYGNNNHMLKEVIKTKLDLNSEIIESISGLSAETL
                     PDGFELSKYLNNVLTLFSNNKEQALNKSCSSKLKNNYGYAAILLVIAAASTVMMGFFN
                     SRAAMKNELTAMNAFIHDGKNNEIYSVIEEIKNETDYLKFYLQQAQELKAAAAANDYV
                     DTNILREVYRIKPFETRVTSIYSGRDSTQLLCLSPSMSEAALFFSDLKEIEQVKSAYM
                     PAVQSKSGEPFSYSIVLKLKEVSSEE"
     gene            complement(1800..2216)
                     /locus_tag="PKH42_09555"
     CDS             complement(1800..2216)
                     /locus_tag="PKH42_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOA20720.1"
                     /translation="MLSAYPACIYKEKDGGYSVIFPDLNHLATCGETLDEALAMAVDC
                     LAGYLFEAEKEGTTVAPPSNMNDIDISAEYDNYVQAFVNIITVDVEEYAKKHFERSIK
                     KTLTIPLWLNNMAVSRDINFSQVLQSALKEKLNIRE"
     gene            complement(2258..2443)
                     /locus_tag="PKH42_09560"
     CDS             complement(2258..2443)
                     /locus_tag="PKH42_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOA20721.1"
                     /translation="MTKFRDLEKLILTDGWRLKSCVGSHFQYVHPIKPGKVTIPSHTG
                     DIPKGVVNSVLKQAGLK"
     gene            complement(2499..3236)
                     /locus_tag="PKH42_09565"
     CDS             complement(2499..3236)
                     /locus_tag="PKH42_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOA20722.1"
                     /translation="MIIVFFITGLLIGSFINVCIYRIPLHKSIIKPPSSCATCGHRLN
                     YYDMLPVANYVINSGKCRYCGERYSIEYPLIELFNGLFYGAIAVKYGFGLALVLYCIL
                     GSILIAVSVIDLKHKIIPDSLNISVAAIGIVFVLYDGSIILDRVFGAFIGFALFFAIA
                     LATDAMGGGDIKLIAGLGLVFGVQGVLFIMFFSFVIGAVISVILLITKFKSRKDEIPF
                     GPFISTAALLYIFYGSEIINLYLSAVL"
     gene            complement(3309..3737)
                     /locus_tag="PKH42_09570"
     CDS             complement(3309..3737)
                     /locus_tag="PKH42_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOA20723.1"
                     /translation="MGRSINKLRSKKGFTLIELIVVLAVLAIIMAIAVPRFVGVQEEA
                     KVESDRATLANIVKISEFYLVKGEMVKGVVGTPQEYTSETTPSLESIIGEDFPAIEFQ
                     SEGNEDAVLADVTVSITDLGVVSAEVGVEGEGTTLVKYPE"
BASE COUNT         1249 a          842 c          516 g         1307 t
ORIGIN      
        1 tttcttcata caagcttttt agatttatgt cctggttcat attttcatat tcagcataaa
       61 tatttttgta ttcatccccc actgatagct ttgcaaatac cctgaaaggt attccggaca
      121 caaaaaaacc tattattgca actatgaata ttaaaactat cggtttgaat ttaacattat
      181 ttttcaccac tgcccacttc cttcagtatt aaattaatta taaaaattac cgcccaattc
      241 atcccttcgg tagcaaacta aatataagaa ttacctctca catctttcag tttcaataca
      301 accctagtgc ttactttctt cggtttcaat actaataatc agaatcaaca gtttgaatat
      361 aaaattattc ttcactgctc acttccttca atttcaacac tattgaatag gaaaatggct
      421 cgccggattt tgactgaacc gccggcatat aagcactctt aacctgttca atttccttca
      481 aatcagaaaa aaataaggca gcttcactca tggaaggaga aaggcacaaa agctgtgtgc
      541 tgtccctgcc cgaataaatt gaagttaccc tggtttcaaa aggttttatc ctgtaaactt
      601 ctcttaaaat atttgtatca acataatcat ttgctgccgc tgccgcctta agctcttgag
      661 cttgctgtaa ataaaatttc aaataatccg tttcattttt tatttcttca attacactgt
      721 aaatttcatt gtttttcccg tcatgaataa aagcattcat tgctgtcagt tcatttttca
      781 tggcagcccg ggaattgaaa aaccccatca tcaccgtgga tgctgcggca attaccaata
      841 aaatggcagc ataaccatag ttatttttaa gctttgagct gcagctttta ttcagtgcct
      901 gctctttgtt attagaaaac aaagttaaaa cattgtttaa gtatttgctc aactcaaaac
      961 catcaggaag cgtttctgca gataaacccg aaatgctttc aataatctca ctgtttaaat
     1021 ccagtttggt tttgattact tctttgagca tatgattgtt attgccgtaa atataaattt
     1081 tttctataga cctgttgccg cttacagaat aataataccg cataaacttg gttagttgtg
     1141 cgacaatttc ctctagatca gaactaatat gaaaatcaca aaatccatta tgtgcagtcg
     1201 aaaaggaaat gaagttttta ttcacactaa taaatgccgc attttcacca ttgtttaaac
     1261 caacatcatt aatcttaata ttatttttat acaatgaatt gatacaatta tgggaaatat
     1321 caattttcaa tagtttaagc ttcagctttg cagccagctc aacacactca ctgactaaaa
     1381 tcaccggaac gcaatacaca atatactcgg catatggtat tctattctca tttgtagcat
     1441 tggatatttc atacactatt ttgtacttgc ttaaatcgac cgaaacaatt tgctgcagtt
     1501 ctatttgaag cattgataaa atttccgggc tcttctttaa taaaggcagc ttaatttttc
     1561 taatcataat tttgcttgaa ttaattacaa aaattgcctt ccttgttttt attccattat
     1621 tcttaaattc ctcattaatt acattgacaa tcccatccat gtcaataatt ttaccatcct
     1681 tggcaccaga actctcaata gtcatacacc ttagaacaga cagtgtttca cctttttttg
     1741 aagcctcaat aatttttata ttattatcta tatcaataga caaaatcact gccataatat
     1801 cactccctga tattcagctt ttcctttaat gccgattgta aaacctggga aaaattaata
     1861 tccctgctta cagccatatt gtttagccac aatggtattg taagtgtttt tttaattgac
     1921 ctttcaaaat gcttctttgc atactcttct acatcaacag taataatatt aacaaatgct
     1981 tgaacataat tgtcatattc tgccgatatg tctatatcgt tcatatttga tggaggagcc
     2041 actgtagtac cttccttttc agcttcaaac aaataacctg caagacaatc cacagccata
     2101 gccaaagctt catccaaagt ttcaccgcag gtagccaaat ggtttaagtc tggaaaaata
     2161 accgaatacc ctccatcctt ttccttataa atacacgccg gatatgcaga caacataata
     2221 atttaccttt ccttttttta atagtatgac atacctctca tttcaatcct gcctgtttca
     2281 gcacagagtt gacaactcct tttggtatat cacccgtatg gcttggaata gttactttcc
     2341 cgggttttat aggatgtaca tattgaaaat gtgatcccac acaagatttt agcctccacc
     2401 cgtcagttaa tattagcttt tctaaatctc taaatttcgt aataaccact ccaatactat
     2461 tatattatcc aatacgtatt atgtcaatac gtattaaatt ataaaactgc cgataggtat
     2521 aaattaatta tttcagagcc atagaatatg tatagtaatg ctgctgttga aataaatggt
     2581 ccaaagggga tttcatcttt tctgctttta aattttgtaa tcaataaaat aactgaaatt
     2641 acagcaccaa ttacaaatga gaaaaacata ataaacaaaa ccccttgaac accaaataca
     2701 agacccaatc cggctataag ctttatgtct cctccgccca tggcatcagt tgccaatgca
     2761 atggcgaaaa acaaagcaaa tcctataaat gcaccaaata ctcggtctaa tataatcgaa
     2821 ccgtcataca atacaaacac aataccaata gcagctactg aaatatttaa actgtcagga
     2881 attattttat gctttaggtc aataacactg actgcaatga gtatgctccc taatatgcag
     2941 tacaaaacaa gagctaaacc aaaaccatat tttacagcga ttgctccata aaacaacccg
     3001 ttaaataatt caatcaaagg atactcaatt gaatacctct caccgcaata cctgcacttg
     3061 ccgctgttta ttacatagtt agctacggga agcatgtcat aatagtttaa tctgtgcccg
     3121 caggtcgcgc aggaggatgg cggttttatt atggacttat gtaatggtat gcggtagata
     3181 catacattta taaatgaacc tatcaataaa ccggttataa aaaatactat tatcatagat
     3241 tgtccttatt tttcctattg ggacattcct ttgtatcccc ttgatattta tggtgtaggc
     3301 actacgattt attcaggata ctttacaagt gtagtgcctt ctccttctac accaacttct
     3361 gcagatacta ctcctaaatc tgttatactt acagttacat cagctagaac agcatcctcg
     3421 ttcccctcag actgaaactc aatagcagga aaatcttctc caattattga ttctaatgat
     3481 ggagtagttt cacttgtata ttcctgtggt gtccctacaa ctccttttac catttcaccc
     3541 ttcaccaaat aaaactccga tatcttaaca atattagcca aagttgccct gtcagattcc
     3601 acttttgctt cctcctgcac ccccacaaac ctcggcactg caatagccat aattatggca
     3661 agcaccgcca acaccacaat caattctatc aacgtaaacc cctttttgct gcgcagctta
     3721 ttaatgcttc ttcccatctt tttactctcc ttttcttttt attaataaca ttcaagagat
     3781 tagcaagatg caaacattat gtttcttgaa ttttattttc attgtacagt tttcaccatt
     3841 tcaaacatgg gcaggaccat tgatattaca ataaacccta ttacaattgc cataaccact
     3901 atcattaatg gctc
//
LOCUS       DAOPUE010000155         3890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_23641_length_3890_cov_9.409909, whole genome shotgun sequence.
ACCESSION   DAOPUE010000155 DAOPUE010000000
VERSION     DAOPUE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_3890_cov_9.409909"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..973)
                     /gene="hprK"
                     /locus_tag="PKH42_09575"
     CDS             complement(62..973)
                     /gene="hprK"
                     /locus_tag="PKH42_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOA20724.1"
                     /translation="MQYVKLEDLVSYTGIEPVYYPEDVQSRIYTPDIVRPGLQMAGYF
                     EWFSFERVQVMGKAEILYIETLDEEVKKERLDKFFSFPIPALIVANEMEVDDLIIYYA
                     KKYKRPVFKSNERTDKLVNIMINFLEEELAEETTLHGVCLEVFGVGVLLRGKSSVGKS
                     ETALELINRGHRLVADDAVIIKKVEGGLKATCPELTRHLMEIRGIGILDIQHLFGVGS
                     IRIEQFVELVVDLEEWDENKEYDRIGKDEEYTEILGVKVSTVIIPVRPGRNTSAIIEI
                     AAKNYRQKLLGFNPLDTYNKKFRNMIR"
     gene            complement(1015..2847)
                     /gene="uvrC"
                     /locus_tag="PKH42_09580"
     CDS             complement(1015..2847)
                     /gene="uvrC"
                     /locus_tag="PKH42_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOA20725.1"
                     /translation="MFNIEEELKKLPDSPGVYQMKNAFGEIIYVGKAKNLKKRVRQYF
                     QSKNHIPKIQAMIKNIYEFEYIITDNEVEALILEANYIKKYRPKYNTLLRDDKQYPYI
                     KISVQERYPRIYKVREVKKDGAKYFGPYPSAYAVNEIIDIIGNLYKLRKCSLKLDGTK
                     KSQRPCVYYHINRCTAPCSGNVDIKEYSQEVNKATALLTGSEGIIKSLNEKMIEASEN
                     MEYEEAAKYRDQIKALEVISEKQKVVSQAGIDQDVIGSAIGIDEACIQIFFIRNGKII
                     GSEHYLLTNIENETREQIISSFLTQFYTGTVYIPKEIYIETKIEDMEVFEKLLSQKRG
                     NKVTLKVPVKGEKSMLIKMIKKNALEILEKGSRRIKKNIDEGMEAIKELSNVLNLDKT
                     PERIEAYDISNIQGVESVGSMVVFENGIPNKSNYRRFKIKTVEGPNDYKSLEEVLERR
                     INRGLGDGKQGFNKMPDLILIDGGKGQTNIAEEVISNFGLSIPVCGMIKDDKHTTKGL
                     IYKNKEIELRKTDIVYKLIYRIQEEAHRFAIEYHRNLRDKTLFKSELDNIKNIGEKRK
                     INLLKKFGSIENIKTKSINDLLSVPGMNTVAAESVYNYFHKEKN"
     gene            complement(3058..3780)
                     /gene="nagB"
                     /locus_tag="PKH42_09585"
     CDS             complement(3058..3780)
                     /gene="nagB"
                     /locus_tag="PKH42_09585"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOA20726.1"
                     /translation="MEVIIKNNYEEISKLAADYLINTVKAKNNAILGLPTGSTPIGMY
                     QEVINRYKDNISFQNVRTFNLDEYVGLDKSNINSYRYFMDENLFSHIDIKEENIHIPN
                     GVARDIEKECINYENLLKTTGQMDIMYLGIGHNGHIGFNEPDEFFEPYTHIVKLTEDT
                     IESNKRFFDNIESVPQTAITMGIKTIMSAKKIILLASGESKAEAILKTVKGKITPRVP
                     ASILQLHNDVTLIIDKDAAKFL"
BASE COUNT         1111 a          762 c          483 g         1534 t
ORIGIN      
        1 tactcttaaa ttatatctta aataattatt taatgcaaca ctaaaaaata aagttgcatt
       61 tttaccgaat catatttcga aattttttat tgtaagtatc aagaggatta aaaccaagaa
      121 gtttctgtct gtaatttttg gcagctattt caataatagc tgatgtattt cttcccggtc
      181 tgaccggtat aataaccgta gataccttaa cacctaatat ttccgtatac tcttcatcct
      241 tgccgattct gtcatattcc ttgttttcat cccactcttc caaatcaacc accaattcta
      301 caaactgctc aatcctgatg gagcctacgc caaacaaatg ctgaatatca agtattccta
      361 taccccttat ttccattaag tgtctggtta attcagggca ggttgcttta agccctccct
      421 caaccttttt tattattacc gcatcatcag ccacaagtct gtgccccctg tttatcagct
      481 ctaaggctgt ttcgcttttg ccgacactgc ttttccccct taataatacc ccaaccccga
      541 atacttccaa gcaaacaccg tgcaaagttg tttcttcagc caattcttct tcaaggaagt
      601 taatcattat attaactaat ttatctgttc tctcattaga tttaaaaaca ggtcttttat
      661 attttttcgc ataataaata ataagatcat caacttccat ttcatttgcc acaattaatg
      721 ccggtatagg aaagctgaaa aatttgtcca gtctttcttt ttttacttct tcatccaatg
      781 tttcgatata taaaatttct gcttttccca ttacttgaac tctctcaaag gaaaaccatt
      841 caaaatatcc tgccatttga agacccggcc ttactatatc aggtgtatat attctggatt
      901 gaacatcctc aggataataa accggttcta ttcccgtgta actgactaaa tcttcaagtt
      961 ttacatactg catattgtac catccctcta tattaagtaa tcataatcta atgactagtt
     1021 tttttccttg tgaaaataat tgtaaaccga ttctgccgct acagtattca ttccgggtac
     1081 cgacaaaaga tcattaatac ttttagtttt aatattttca attgagccga attttttgag
     1141 taaattaatt tttctttttt ctccgatatt tttaatatta tcaagctctg atttaaacag
     1201 tgtcttgtcc cttaaattcc tgtggtactc aattgcaaat ctatgagctt cttcctgaat
     1261 cctgtaaatt aatttataaa ctatatcggt ctttctgagt tcaatttcct tgtttttata
     1321 tattaatcct ttagttgtat gtttgtcatc cttaatcata ccgcaaacag gaatacttaa
     1381 accaaaatta cttattactt cttctgcaat atttgtttgt ccctttccgc cgtcaattaa
     1441 aattaagtcg ggcattttgt tgaaaccttg cttgccatct cctaaccctc tgttaattct
     1501 tctttccaat acttcttcca aacttttata gtcatttggt ccctctactg tctttatttt
     1561 gaaacgacgg taattgcttt tattgggaat tccgttttca aaaacaacca tggagcctac
     1621 agattcaact ccttgtatat tcgaaatatc ataagcttca atcctttcgg gagttttatc
     1681 caaatttaaa acattactca gctctttgat tgcctccatg ccttcatcaa tattcttctt
     1741 tattctcctg cttccctttt ctaatatttc aagagcattt ttctttatca ttttaataag
     1801 catgctcttt tcgcctttaa ccggaacttt taaagtaacc ttgtttcctc ttttttggga
     1861 caagagtttt tcaaaaacct ccatatcttc tattttggtt tctatatata tctcttttgg
     1921 aatatatact gtacctgtat agaattgtgt taaaaatgag ctgataattt gttcccttgt
     1981 ttcattttct atattcgtaa gcaaataatg ctcgctgcct ataattttac cgtttcgaat
     2041 gaagaaaatc tgtatgcagg cctcatcaat accaattgcg cttcctataa catcttggtc
     2101 tattcctgct tgagaaacaa ctttttgttt ttctgaaatt acttcaagag ccttaatctg
     2161 gtctctatat ttggcagctt cttcgtattc catattttct gaagcttcaa tcattttttc
     2221 gtttaacgat ttaataatgc cttcactgcc tgtcaataga gctgttgctt tatttacttc
     2281 ttggctgtat tcttttatgt caacgtttcc actacagggt gcggtacacc tgtttatatg
     2341 ataatatacg catggtcttt ggcttttctt tgtaccgtca agttttaaag agcattttct
     2401 aagcttgtat aagtttccta taatatctat tatttcgttt acggcataag cactcggata
     2461 gggtccgaaa tattttgccc catctttttt tacttctctg accttataaa ttcttgggta
     2521 tctttcttga acagatattt taatatacgg atattgctta tcatctctta aaagagtatt
     2581 gtatttaggt ctgtattttt ttatataatt tgcttccaga atcagagctt caacttcatt
     2641 gtccgtaata atatattcaa actcataaat attttttatc atagcctgaa ttttaggtat
     2701 atgattttta gactgaaaat attgtctgac cctctttttt aaatttttag ccttgcccac
     2761 atatattatt tcaccaaaag catttttcat ctggtaaaca ccggggctat ctggcagttt
     2821 ctttaattct tcctctatat taaacatttt tactcctgat aattactaca tatattatac
     2881 cacatttgcc ttattattaa cataaataat aaattaattt tatattaaga atattacacg
     2941 tctattttta agaaacctgc gcattaactc tatatataaa atgcaccttc aattgactaa
     3001 tcatttgagg tgcacttaaa aattcttaga ttaatattgt ttgttaaaga taataactta
     3061 caagaatttt gcagcatcct tgtcaattat caatgttacg tcattatgaa gctgtaatat
     3121 ggaagccgga acccggggag tgatttttcc ttttactgtt tttaatattg cttcagcctt
     3181 tgactctccc gaagccagaa gtattatttt ctttgcggac ataattgttt taatacccat
     3241 ggttattgca gtctgaggca cactttcaat attatcgaaa aatcttttat tagattctat
     3301 agtatcttca gtaagtttta cgatatgagt atagggctca aaaaattcat ccggctcgtt
     3361 aaaaccgata tgtccgttat gtcctattcc aagatacatt atatccatct gtccagttgt
     3421 ttttaataaa ttttcataat ttatgcattc cttctcaata tcccttgcaa caccatttgg
     3481 aatatgaata ttctcttctt ttatatctat atgagaaaac aaattttcat ccataaaata
     3541 tctgtagctg tttatgtttg atttatctag tcctacatat tcatccaaat taaatgttcg
     3601 cacattttga aatgaaatgt tgtctttgta ccgattaatt acttcttgat acatgccaat
     3661 cggtgttgaa ccggtaggaa gtcctaagat agcattattt ttggctttaa ccgtattaat
     3721 taaataatct gctgccaatt tacttatttc ctcataatta tttttgataa ttacttccac
     3781 tttttcaatc cattcctttc tatatattga ttatttgata tacccaaatt attttttgat
     3841 ggtatcacaa aaaatgcaaa taaacaataa aaatttggca aaaaacaatc
//
LOCUS       DAOPUE010000156         3881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_23723_length_3881_cov_9.562206, whole genome shotgun sequence.
ACCESSION   DAOPUE010000156 DAOPUE010000000
VERSION     DAOPUE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23723_length_3881_cov_9.562206"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..690)
                     /locus_tag="PKH42_09590"
     CDS             complement(49..690)
                     /locus_tag="PKH42_09590"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOA20727.1"
                     /translation="MKIKIIYPILILTLAMSFIITYGISEGKFETYAFAEIFEKSEEA
                     EESEAYDEIKDIDEITARNMEAKLTLKELKYHIERDSLEQETSLEYKNDQYKKETTSR
                     GGFRFNNAMDMVATAYDLSYESCGKYPDDPEYGITASGTKAQPGTVAVDPDVIPLGTK
                     LYIASTDGSPDYGFATALDTGSAIKGYRIDLFMENNQDAMDFGRRQVKVYILD"
     gene            complement(1037..1906)
                     /locus_tag="PKH42_09595"
     CDS             complement(1037..1906)
                     /locus_tag="PKH42_09595"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOA20728.1"
                     /translation="MKEIPKIMAVQDMSGVGRCSLTVIIPIMSALGCQVCPLPTALLS
                     NHSEYKEFYFFDFTDHMEEYFSYWEKNDFWFDCVYSGFIGSEKQINIIVDIIEKVKNK
                     NNKALIVIDPVMGDCGCTYATYNSEMITKMSQLVKKADIITPNLTEACILLGRKYYSD
                     KITLPQTKECLKDLCSLGPKISLITGIITDEEEHINVCYDSEQDKYWLAPFSYIDKRY
                     PGTGDLFTSLFLGYYMKGKSLPEAMEEAARFVSLSVRISSEAEAPSSEGVIFEKIMKE
                     LYNEISEYKYTSI"
     gene            complement(2333..3823)
                     /locus_tag="PKH42_09600"
     CDS             complement(2333..3823)
                     /locus_tag="PKH42_09600"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOA20729.1"
                     /translation="MAFIINEPSRTFGEYLLLPNLTTKDCIVENVDLSTPLTRFKRGE
                     APEIKLNIPFSSAIMQSVSDNNMAVALAKNGGISFIYCSQPIESEAEMVRKVKKYKAG
                     FVISDSNLTPEHTLADVLNLKAITGHSTMAITEDGSPTGKLLGIVTGRDYRTSRDSLD
                     KKVKDFMTSREDLIVGREGITLSEANDIIWEHKLNSLPIVDKDFNLVYMVFRKDYSED
                     KENPNSLLDAKKRYVVGAGINSRDYKERVPALVEAGADILCIDSSEGFSEWQHETINW
                     IKQRYDGKVKVGAGNVVERDGFKYLADAGADFVKVGIGGGSICITREQKGIGRGQASA
                     VMDVAAARDEYFEKTGEYVPICSDGGIVLDYHITLALAMGADFVMLGRYFAGFDESPT
                     NKIKYGSGYVKEYWGEGSNRARNWARYDLGGNAGLHFEEGVDSYVPYAGKLDDGLKTT
                     LHKIKSTMCNVGTKTIKELQQNARLTVVSATSLTEGGAHDVILKNQ"
BASE COUNT         1123 a          826 c          564 g         1368 t
ORIGIN      
        1 cttcggggac atttctcgca tgtccccata acttttaact taatgatatt agtctaatat
       61 atagactttt acctgtctcc tgccaaaatc cattgcatct tggttgtttt ccataaacaa
      121 gtctattctg taccctttta tcgctgagcc tgtatcaagg gctgttgcga aaccatagtc
      181 aggactgcca tcagtagaag cgatatacag cttcgtacct aaaggtatta catcagggtc
      241 taccgcaacc gtacccggct gagcttttgt gccggatgct gttattccgt attcagggtc
      301 gtccggatat ttcccacagc tttcatatga taaatcataa gctgtagcta ccatatccat
      361 tgcattatta aatctgaaac caccacgaga ggttgtttct tttttatatt gatcgttttt
      421 atattctaaa gatgtctctt gttctaaact gtctctctca atatgatact ttaattcttt
      481 taatgtaagt tttgcttcca tattccttgc agttatttca tcaatgtctt taatttcatc
      541 atatgcttct gattcttcag cctcttccga cttttcaaaa atctctgcaa aagcataggt
      601 ttcaaactta ccttcactga taccataagt tataataaag ctcattgcca acgttaaaat
      661 caaaataggg tatattattt taattttcat attaccacca accttatatt tgaattgact
      721 aagacgtcta ttaaggtata aatttcttcc ggaaatttat gttttgcata agtatgagct
      781 tatgcattat tggcagtccc tttttaaaaa actcataatt tttggcataa ggagaattat
      841 actttaaaaa aaaagcccgg tcaagtcttt ttttgaaaaa tctgccttct aatgcctata
      901 ttgttgcaat tgcctacttt ttatccaatc ctttatttta acttttctgc tataataagg
      961 cagaattttt agaacatttt ccaaaaatgg catacaaaaa cagcctgtaa aaggctgttt
     1021 ttcactgcaa tatttttcat atactcgtat atttatactc cgaaatttca ttgtacaatt
     1081 ctttcataat cttttcaaaa attactcctt ctgagcttgg tgcttcagcc tcggaactta
     1141 tacgaaccga aagcgaaaca aatcttgctg cttcttccat tgcttcagga aggctcttcc
     1201 ctttcatata ataacccaag aataaggagg taaataaatc ccccgtaccc ggatatcttt
     1261 tatctatata gctgaaaggt gccagccaat acttgtcctg ctcgctgtcg taacaaacat
     1321 tgatatgttc ctcctcatct gttataattc cggttattaa tgatatttta ggtcccagac
     1381 tgcataaatc ctttaggcat tcctttgttt gaggaagagt aattttgtcg ctgtaatatt
     1441 ttcttccaag caatatacaa gcttctgtca aattcggtgt tataatatca gcttttttta
     1501 ccaattgtga catttttgta atcatttcac tgttatatgt ggcataagtg caaccgcagt
     1561 cacccattac agggtctatc acaatcaatg ctttattatt tttattctta accttttcaa
     1621 taatatcgac aataatgttt atttgttttt cagagcctat aaacccgctg taaacacaat
     1681 caaaccaaaa atcattcttt tcccagtatg aaaaatactc ttccatatgg tctgtgaaat
     1741 caaaaaaata aaattccttg tattcggagt gattgcttaa taaagctgtt ggtaaggggc
     1801 acacctgaca tcccaaagca gacataatcg ggataatcac agtcaatgag catctcccta
     1861 ctcccgacat atcttgaaca gccataattt taggaatttc tttcataatt tatcctttca
     1921 ttatagggat tttacctgat acggggacat tccttcagtc cccatacata ttttaactta
     1981 ttcttgtact ttcaaaaggt acagatttat caaattttaa gaggacagtt ttcatatcat
     2041 acgcattgaa tgcatcaaat ttactaaaaa aaatgatttc tatataaata gaaatcattt
     2101 tttacagggt aaagatttat taaataactc tttacaaggg gggctaattt atttcataat
     2161 atatattcga gcttgtacaa tgtttttttg gtgttattct caaaaaatat tttaccagtt
     2221 ttttacacca tattctacaa aattctctac atatagtgta gcaaatccat tgcacatttg
     2281 caagaaaaat tatttgaaaa ttagaaattt ctgtaaagtt ttactaatct aactattgat
     2341 tcttcaaaat tacatcatga gctccgcctt cggtaagact tgttgcagaa accacagtaa
     2401 gccttgcgtt ttgctgaagt tcctttattg tttttgtacc aacgttgcac atggtcgatt
     2461 ttattttgtg aagtgttgtt ttaagaccgt catcaagctt gcctgcataa ggaacatatg
     2521 aatcaacgcc ttcttcaaaa tgaagtcccg catttccgcc taaatcatat cttgcccagt
     2581 ttcttgccct gttggagcct tctccccaat attcctttac gtatccgctc ccgtatttta
     2641 ttttatttgt agggctttca tcaaatcctg caaaatacct tccaagcatc acgaaatcag
     2701 ctcccattgc caaagcaagg gttatatgat aatccaggac aattcctccg tcagagcata
     2761 ttggaacata ttctccggtt ttttcaaaat attcatcacg ggcagcagca acatccatta
     2821 cagccgaagc ctggcctctt cctattccct tctgctcacg cgttatgcat attgagcctc
     2881 cgcctatacc taccttaaca aagtcagcac ctgcatcggc caaatattta aaaccatccc
     2941 tctcaactac attgccggca cctaccttaa ccttgccgtc atatctttgc tttatccaat
     3001 taattgtttc atgctgccat tcactaaaac cttcggatga gtctatacaa agaatatccg
     3061 ctccggcttc aactagggca ggaacccttt ccttgtaatc tcttgaatta atacctgctc
     3121 ccacaacata tcttttctta gcatccaaca aggaattagg gttttccttg tcttcggaat
     3181 aatcctttct aaataccatg tataccaagt taaaatcttt gtctacgatg ggaagagaat
     3241 tcaacttgtg ctcccaaatt atatcattgg cttccgaaag agtaatacct tctcttccta
     3301 cgattagatc ttctctgctt gtcataaagt ccttaacctt tttatccagg gaatcgcgag
     3361 atgtcctgta atccctgccg gttacaattc caagaagctt tcccgttgga gagccgtctt
     3421 ctgttatggc cattgtcgaa tgaccggtta tagctttcaa atttaaaaca tccgctaagg
     3481 tatgctccgg tgttaagttg gaatcgctta ttacgaatcc ggctttatac ttcttaactt
     3541 tccttaccat ttctgcttca ctttctatcg gctgtgagca atatataaaa gatatacctc
     3601 cgtttttcgc caaagctact gccatattgt tatcggaaac agattgcatg atagctgaag
     3661 aaaaaggtat attaagcttt atttcaggag cttcgcctct tttaaaccga gttaacggtg
     3721 tacttaaatc aacattctca acaatacagt cttttgtggt caaattaggt aaaagtaaat
     3781 attctccaaa agttcttgaa ggctcgttta taataaatgc catcttaatc tccttttctt
     3841 attgttaatc gttttttaat aattgctatc ataccatatt a
//
LOCUS       DAOPUE010000157         3811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_24299_length_3811_cov_12.578009, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000157 DAOPUE010000000
VERSION     DAOPUE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24299_length_3811_cov_12.578009"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..514
                     /locus_tag="PKH42_09605"
     CDS             176..514
                     /locus_tag="PKH42_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20730.1"
                     /translation="MKKILFYGMTGEKMCFQHILMNALDLKTAGAEVKIIFEGASVKL
                     VSKFEDENNPLYRKAKEAVLIDGICLACSKVLDVYEANLKTGLPMLNDMSGHAGMKKY
                     LEDGYEVISM"
     gene            925..2337
                     /locus_tag="PKH42_09610"
     CDS             925..2337
                     /locus_tag="PKH42_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HOA20731.1"
                     /translation="MEGEKKKRKGFEMPHGFIVILLVIIACCVLTWILPAGQFERAYD
                     EALDRDIVVPGSYHAVEASPVGIWGFFQSLYTGMVEASDIIFFVIFAAGYVYLLMKTG
                     SLNAMVGAILRKLGTNDHLLIPIFMIIFGIAGATFGMYEETYGLIPAFIIIAITLGYD
                     RLVGSAIVFVGVATGFAAAILNPFTIGIASNIAGIPLVSFKMTLFRVVCFILFEGLSI
                     AYVMQYARKVRKDPTKSILYGTVENTDFTKDALTRDEVLKLPFTSSQKISLLIFVVLI
                     CVIGFTVAVFGFYLTELAAMFIVAMILTGVVNKMSSREICEIFIESTAISMYGSLFIG
                     LSRSCSVVLEQSNVIDTAILYLSNAISGFPAALHSIGMLIVQNIIGFFITSGSGMAVV
                     TMPIMAPLSDLIDMSREMAVTAYQFGDGFSNMFWPTAVATECGIIGIGMDKWYKFITP
                     LFGMMFLLQCIMMIAGGFIF"
     gene            2353..3564
                     /locus_tag="PKH42_09615"
     CDS             2353..3564
                     /locus_tag="PKH42_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005981880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOA20732.1"
                     /translation="MIKVELKNELYKIEDNLKSVFEEISDFIFKHPELGLEEFISSKY
                     LVELMKNYGFSVTYPYCNIETAFLAEYGDDDGTTIAFLAEYDALPGYGENSDQNAHAC
                     GHNWISAATAGAAIVLSQLKQKYNFKGKIVLVGTPAEETVGSKADMVKAGGFEKIDIC
                     LQPHFGEFNDICCNVQAMDSIEFEFKGKATHAAASPYEGINALDAAMLTFAGINALRQ
                     HVKSDVRIHGIITEGGIASNIIADKSVCKIGTRAMERKDLNKITKKVINIAKGAQLMT
                     EAEMSYKFYENSLDNLVNIPLLIDLTKSNLEAAGIKDIYYDGSTPPSGSTDLGNVSYV
                     CPTQYMEIALETSETANVHEESILKYVNSEEAYDKLHKTIKAMAATAIDLYMDDYLVK
                     DIKDWHKNKIS"
BASE COUNT         1284 a          552 c          761 g         1214 t
ORIGIN      
        1 agtataaggc agaaaataat aaaaatattt cacaaaatat ttttattatt tttttcataa
       61 agacaaaatt tcaagaaaaa aataggctgg ttatgcaaag gacttttctt attgtttaat
      121 tttacatttt atagtaatat agataaagat ggataaaagc acaggaggga taagaatgaa
      181 aaagatttta ttttatggga tgaccggtga aaagatgtgc tttcagcata ttttaatgaa
      241 tgcattagat ttgaaaactg caggagcaga agtaaagata atatttgagg gtgcatcagt
      301 taaactggtt tcaaaatttg aagatgaaaa taatccgctt tatagaaagg caaaggaagc
      361 ggtgttaata gacggtattt gccttgcatg ttcaaaggtt cttgacgtat acgaagctaa
      421 cctgaaaaca ggtttgccaa tgcttaatga tatgtcaggt catgcaggaa tgaagaaata
      481 cctggaagat ggatatgaag taataagtat gtaacaaata catataaaaa accagagtca
      541 gacaattgta aagtaaattc tccccattat tttaacggta atggggagat tttttatgtt
      601 aaggcagtgg caaagctcta tatcagatct gtatgtaatt tagaaaagac atttatacaa
      661 acctataaac tatgatacca tctcaagtaa tgcttctgat acagttttca aaaagtaaca
      721 agtaagctaa gaaatcttag ctgctttttt cgctaaaata ttattcaata tatagaattt
      781 ttttcttgat aattgtaaaa acaaggatta ttatatgctt gaaaactata acatcatcag
      841 taggaatggg attaaagcct ataaggatgt ttattatttt tcagacattt gagctaaaaa
      901 taaattatat gagaggagtt ttttatggaa ggggaaaaga aaaaaagaaa aggttttgaa
      961 atgcctcatg gatttattgt aattttatta gttattattg catgctgtgt tttgacttgg
     1021 attttgcctg caggtcaatt tgaacgtgct tatgacgagg cattggacag ggacattgtc
     1081 gtaccaggca gctaccatgc agttgaagca agccctgttg gaatatgggg attctttcag
     1141 tcactttaca caggtatggt ggaagcgtcc gacataatat tttttgttat ttttgcggca
     1201 ggatatgtgt atttgttaat gaagacaggc tccttaaatg caatggttgg agcaatttta
     1261 agaaaactag ggaccaatga ccacctgctt attccaatat tcatgattat ttttggtata
     1321 gcaggtgcta cattcggtat gtatgaagaa acctacgggc ttatacctgc attcataata
     1381 attgcaataa ccttaggcta cgacaggctg gtgggaagtg ccatagtttt tgtaggggtt
     1441 gccacaggtt ttgcagcagc aatattaaat ccctttacta tcggtatagc cagcaatatt
     1501 gccggaatac ctttagtatc ctttaaaatg actcttttta gagtagtatg tttcatattg
     1561 tttgaaggat tatcaattgc ttacgttatg cagtatgcta gaaaagtcag aaaggatcct
     1621 acgaaaagta ttctttacgg aaccgttgaa aatacagact ttacaaaaga tgctttaaca
     1681 cgtgatgaag tccttaagct tccctttacc tcaagtcaga aaatatcttt gctgatattt
     1741 gttgtactaa tatgtgtaat aggctttact gtggctgttt ttggatttta tctgacagaa
     1801 ctggctgcta tgtttatagt tgccatgata ttgacaggtg tagtcaataa aatgagttcc
     1861 agagaaatat gtgaaatatt catagaatca acagccatat ctatgtatgg ttcattattt
     1921 ataggactgt cccgttcatg ctctgttgtg ttggaacaat caaatgttat agatactgca
     1981 atactttatc tgtcaaatgc aatcagtggc ttcccagctg cattacattc aataggcatg
     2041 ctaattgttc aaaatataat aggatttttc attacctccg gaagcggaat ggctgttgtt
     2101 acaatgccta ttatggctcc attatcagac cttattgata tgagccgcga aatggctgtt
     2161 acggcatatc agtttggtga cggattctcc aatatgttct ggccgacggc agttgcgaca
     2221 gaatgcggta taatcggtat aggtatggat aaatggtaca aattcataac tcctttattt
     2281 ggaatgatgt tcttattgca atgcataatg atgattgccg gaggcttcat attctaattt
     2341 taggaggatg caatgataaa ggtagaatta aaaaatgaat tatataaaat agaagataat
     2401 ttgaaatctg tttttgaaga aataagcgac tttatattta aacatcctga attaggtttg
     2461 gaagaattta taagctctaa atatttggtt gagcttatga aaaactacgg gtttagtgta
     2521 acatatccgt attgcaatat agaaactgct tttcttgctg aatatggaga cgatgatgga
     2581 actacaatag cattcctggc agaatatgat gcactgcccg gctatggaga aaacagcgac
     2641 caaaatgctc atgcctgcgg acacaactgg atttcagcag caacagccgg ggcagcgatt
     2701 gtactttcac agctgaagca aaaatataat ttcaaaggta aaattgtatt ggttggaact
     2761 cctgcagaag aaaccgtcgg ttcaaaggcg gatatggtta aagcaggagg attcgaaaaa
     2821 attgatattt gtttgcagcc gcattttgga gagttcaatg acatatgctg caatgtccag
     2881 gctatggatt caatagaatt tgaatttaaa ggaaaggcaa ctcatgcagc cgcatcaccc
     2941 tatgaaggta ttaatgctct tgatgctgcc atgctaacct tcgcagggat taatgcctta
     3001 agacagcatg tgaagtctga tgtgagaata cacggcatta ttactgaggg cggtattgca
     3061 tcaaatataa ttgctgacaa gtcagtatgc aaaatcggca caagggcaat ggagagaaaa
     3121 gacttgaata aaataaccaa gaaggttatc aatatagcta agggtgccca gcttatgaca
     3181 gaagcagaaa tgtcctataa attttatgaa aattccctgg ataaccttgt aaatattcct
     3241 ttattgattg acctgaccaa atccaatctt gaagctgcag gtattaagga tatttattat
     3301 gatggctcaa ctcctccttc aggctcaacg gatttgggga atgtaagcta tgtctgtcct
     3361 acacagtata tggaaatagc tcttgaaaca agtgaaacag caaatgtgca tgaagaatca
     3421 atattaaaat atgttaattc tgaagaggca tatgataaac tgcataaaac aattaaagct
     3481 atggcagcaa ccgcaattga tctatatatg gatgattatc tggttaaaga tattaaggat
     3541 tggcataaaa acaaaataag ctgatatatt ttaattaagt ttaacccctt atgacaataa
     3601 ggggttttct atattcttaa gtgtttttta ttgtagttta tttcttgcta tgacacttcg
     3661 ttaaaaacaa aaggaacagg gtaatttcaa acttgaaata tataatgcta atatttatat
     3721 aaatgcggga aaataataaa tattttatta tttttctttt tttaatgttg acaaaattac
     3781 tggggtatct tataatcaat tgtaccctac c
//
LOCUS       DAOPUE010000158         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_24691_length_3766_cov_10.043923, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000158 DAOPUE010000000
VERSION     DAOPUE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24691_length_3766_cov_10.043923"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..379
                     /locus_tag="PKH42_09620"
     CDS             2..379
                     /locus_tag="PKH42_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOA20733.1"
                     /translation="MASIEEVQIILDEIAEELPPEIYKDLNGGIILLPQVKYHKKSVD
                     NDLYVMGEYRNEMISGRYIIVYYGSVERLYGNLTRNRLKNKLRSVVKHEFLHHLESLA
                     GEKHLEIEDERKLSEYLKKKDSF"
     gene            575..1873
                     /gene="lysA"
                     /locus_tag="PKH42_09625"
     CDS             575..1873
                     /gene="lysA"
                     /locus_tag="PKH42_09625"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOA20734.1"
                     /translation="MVREIKGNTLYFDGCNTVELANKYGTPLYVMSETAMVDKCKEIK
                     DTFLNKYERTRAAYASKAFLTLAMCKIIEREGLCLDVVSGGELYTAIKADFPAERIEF
                     NGNNKSIEELELAIDYNIGRIIVDGFDELGIIEDICKKKGKKTNILYRITPGVKSDSH
                     DYIVTGKKDSKFGFPLDDDVIFPAIEKAINSPYINFLGFHFHVGSQLHNNESHLKALK
                     ISLKLIKDTIDKYNYVTQELNVGGGFGIRYTDADDKKPYAYFLDPMMAEIEEFSKRMD
                     IKRPEIVIEPGRSIVGEAGITLYTIGTIKDIEGIRKYVSVDGGMTDNIRPALYQAKYE
                     CVIANKASQPSTDLVTISGKCCESGDILIRDAYIPLAERGDILAIFSTGAYGYAMASN
                     YNKNPFPATVLVKEGKSEVIVKRQTYEHMIENEVIPKSLV"
     gene            1893..2729
                     /gene="dapF"
                     /locus_tag="PKH42_09630"
     CDS             1893..2729
                     /gene="dapF"
                     /locus_tag="PKH42_09630"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOA20735.1"
                     /translation="MEFCKMQGAGNDFIIINNMKLNIPVDKLPAIAKKLCQRKVSIGA
                     DGLMAVDIPDGDADFKMLFFNRDGSIGEMCGNGARCISRYAYINNIAGKKMIFETGAG
                     IVSAEVLEGRLVKVMLNNPEVINLYNDIEIDGIKYELSYIELGNPGLPHAVVKYNLKQ
                     ADENKLREEGIKIRHYEGFPKGANVNFFEVLDENTALVKTYERGVEDFTLACGTGSAS
                     TALALILKGYLKNNQAKMINDGGELIIEVEKTDNKIEKLFLIGDTNIVAIGKIMDEDL
                     II"
     gene            2751..>3766
                     /locus_tag="PKH42_09635"
     CDS             2751..>3766
                     /locus_tag="PKH42_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOA20736.1"
                     /translation="MSTSKKTVWENYRFSVILIISIAIGSFIGLVFGDRAVILKPFGD
                     IFLNLMFTAVTPLVFVTIASAVGSMVNMRRLGRILGNMLLVFVITGLFAAVLIIFVVS
                     VYPPAEGVNIQIEAAGELEKLTFADQAVSAITVNDFSKILSRSNMLPLIVFSILFGYC
                     VSAVGGEDNIVARALDALSKVMMKFISVIMLYAPIGLGAYFASLIGEFGPQLLGAYAR
                     AMVVYYPLCVFYFFVFFALYTYYSGGMEAVKSYFKNIVEPSVTSIATQSSIATLPTEL
                     RAAQRIGVPKDIRDIILPIGATAHMDGTVLSSILKISFLFGIFGQEFAGIGTYLTAIG
                     LSILG"
BASE COUNT         1338 a          522 c          786 g         1120 t
ORIGIN      
        1 tatggcgtca atagaagaag tacaaattat attggatgaa atagcagagg agttacctcc
       61 tgaaatttac aaagacctta acggaggtat tattttgctg ccccaagtaa aatatcataa
      121 aaaaagcgtt gataatgatc tttatgtgat gggtgaatac cgcaacgaaa tgatttcagg
      181 aaggtatata attgtatact atggctctgt cgaaaggctt tatggcaatc ttacaagaaa
      241 caggcttaaa aataaactca ggtcagttgt caaacatgaa tttttgcatc atctagaatc
      301 attggccgga gaaaagcatt tagaaataga agatgaacgt aaattatcag agtatctgaa
      361 aaagaaggat tctttttaat tatatgaatt gaattccttc attaatacta ttgtaaacat
      421 agaaatacgg tttttatttt tgatttatag agccgttttt tttatttcaa taatttatct
      481 tgatttaaag gtaaaggctg cttataatat tgcataatta ttatataaag cgttataatt
      541 tttaatacta acacaaaatt tataaggaga taatatggtg cgagaaatta aaggtaatac
      601 attgtatttt gacggatgca atacggttga attggcaaac aagtatggga cgccgctgta
      661 tgtgatgagt gaaacggcaa tggtcgataa gtgcaaagaa ataaaagata catttttaaa
      721 caagtatgaa agaacaaggg ctgcatatgc ttcaaaagca tttttaactc ttgctatgtg
      781 caagataatt gaaagagaag gcctttgttt ggatgtagta tcgggaggag aactgtacac
      841 agcaataaag gccgattttc ctgcagaaag aattgaattt aacggcaata acaaatcaat
      901 tgaagaatta gagcttgcaa tagattacaa tattggaaga ataattgtgg acggctttga
      961 tgaattggga attattgaag atatttgtaa gaaaaaaggg aaaaagacaa atattcttta
     1021 cagaataact ccgggtgtta aaagtgattc tcatgattat atagttacag ggaaaaagga
     1081 ttccaagttt ggatttccgt tggatgatga tgttatattt cctgcaattg aaaaagcaat
     1141 taattcaccc tacataaatt ttctgggttt tcattttcac gtaggctcac agcttcataa
     1201 caatgaatca catttaaaag cattaaaaat ttcattaaaa ttaataaaag acacaattga
     1261 taaatataat tatgtaacac aagaattaaa tgtcggcgga ggatttggaa taaggtatac
     1321 agatgctgat gataagaagc cctatgcata ttttttagac cccatgatgg cggaaattga
     1381 agagttttca aagagaatgg atatcaaaag acctgaaatt gttatcgagc cgggcagaag
     1441 tattgtagga gaggccggaa taacacttta tacaatcggc actataaagg atattgaagg
     1501 tataagaaaa tatgtgtccg ttgacggcgg aatgaccgac aatataagac ccgcattata
     1561 ccaggcaaag tatgaatgtg ttattgctaa caaagcatct cagccaagca ccgatttagt
     1621 aacaataagc ggaaaatgct gcgaatcggg ggatatattg ataagggatg catatatacc
     1681 attagctgaa agaggagata tacttgccat attttcaaca ggagcatacg gttatgctat
     1741 ggcaagcaac tacaataaaa acccttttcc tgcaacagtc ttggtcaagg aaggaaaatc
     1801 ggaagtaata gtaaaaagac agacatatga acatatgatt gaaaacgaag ttataccgaa
     1861 atcgctagta taaaataagg aagtgagcgc ttatggaatt ttgcaaaatg cagggagcag
     1921 ggaatgattt tataattatt aataatatga agttgaatat tcctgtagat aaattacctg
     1981 ccattgcaaa aaaactatgc caaagaaaag tatcaatcgg agcagacggg ttgatggcgg
     2041 ttgatatccc tgatggagat gcggatttta agatgctttt ttttaaccga gacggaagca
     2101 taggagaaat gtgcggcaat ggagcaagat gtatttctcg ctatgcatat ataaataata
     2161 tagcaggaaa aaaaatgatt tttgaaacag gagcaggaat tgtcagcgct gaagttttag
     2221 aagggcgttt ggtaaaagtt atgctaaata atccggaagt aattaatcta tataatgata
     2281 ttgaaataga cggaataaaa tatgaattat catatataga attaggcaat cccgggcttc
     2341 ctcatgcagt agttaagtat aaccttaagc aggcggatga aaacaaatta cgtgaagaag
     2401 gaatcaaaat aaggcattac gaaggctttc caaagggtgc caatgtaaac ttttttgagg
     2461 tgctggatga aaatacggca ttggtaaaaa cctatgaaag gggggtagaa gactttacat
     2521 tagcttgcgg caccggctct gcgtcaacag cccttgcctt aatactaaaa ggctatttga
     2581 aaaataatca agctaaaatg ataaatgatg gtggggaact gattatagaa gttgaaaaaa
     2641 cagacaataa aatagaaaaa ctgtttctta taggtgatac taacatagta gcaatcggaa
     2701 aaattatgga tgaagattta attatttaaa aattgttaaa ggagaataat atgagtacat
     2761 ctaaaaaaac agtatgggaa aactacaggt tttccgtaat attgattatt tcaatagcta
     2821 tcggtagttt tatcggtctt gtatttggtg atagagccgt aatattgaaa ccctttggag
     2881 atatattttt gaatctcatg tttacagcag ttactccatt agtatttgtt acaattgcaa
     2941 gtgctgtagg cagtatggtc aatatgaggc gtcttggcag gatattggga aacatgcttt
     3001 tagtatttgt aataaccggt ttatttgcag ctgttttaat tatctttgtt gttagtgtat
     3061 atcctcctgc agaaggagta aacatacaga tagaagccgc cggagaatta gaaaaattaa
     3121 cgtttgctga ccaagccgtt tctgcaatta cagtgaatga cttcagcaag attttatcac
     3181 gttccaatat gctgccctta attgtatttt ctattctttt cggctattgt gtaagtgcag
     3241 taggaggaga agacaatatt gtagcaaggg ctttagatgc attatctaaa gttatgatga
     3301 aatttataag tgtaattatg ctttacgcac cgataggact tggagcgtac tttgcgagtt
     3361 tgataggaga attcggaccg cagcttcttg gagcatatgc aagagccatg gtagtttatt
     3421 atcctctatg cgttttctat ttctttgtat tctttgcatt atatacatat tattcgggag
     3481 gaatggaagc ggtaaaatca tattttaaaa atattgtaga accatcggta acatcaattg
     3541 caacacaaag ctcaattgcg acacttccaa cggaactaag ggctgctcag cgtataggag
     3601 tgccaaaaga tatccgtgat atcatacttc caataggcgc tactgcacat atggatggaa
     3661 cagtcttatc atcaatatta aaaatatcat tcttgttcgg catattcggt caggaatttg
     3721 cgggaatagg tacttacctc acagccatcg gtctgtctat attagg
//
LOCUS       DAOPUE010000159         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_25389_length_3691_cov_7.807756, whole genome shotgun sequence.
ACCESSION   DAOPUE010000159 DAOPUE010000000
VERSION     DAOPUE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25389_length_3691_cov_7.807756"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKH42_09640"
     CDS             <1..508
                     /locus_tag="PKH42_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOA20737.1"
                     /translation="DGTHSKINFDGTETASKKPFKERAEVLEGPGINLLDDGRCAYAR
                     FCHRNDGDAWELTEQSDDEHLREEAIIAAKECPAGRLLAVTKNKEDIEPELEPSIDIL
                     QDEELNVSGPIYVKGYIPIESADGDVYEVRNRQTLCRCGKSKIKPFCDSAHVKSNFRD
                     YESDDKNH"
     gene            617..967
                     /locus_tag="PKH42_09645"
     CDS             617..967
                     /locus_tag="PKH42_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20738.1"
                     /translation="MDCPFCCFLRNYIGQYIRIGVVCNCDTNDHIYRDGYITKKSFYN
                     NAIRLYDSPTSGEVIFTACCDSIFEIYIFDTITPTADAKPSDITDIATMVEQRRNLLE
                     SVLGNLQKEETANE"
     gene            960..1175
                     /locus_tag="PKH42_09650"
     CDS             960..1175
                     /locus_tag="PKH42_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20739.1"
                     /translation="MNKYKEDCDICKLLRDFGDRLVFIKFKCPTYFGTVGRILDIRDN
                     LVILEYINGGISVTCCDNICYVVPVAE"
     gene            complement(1273..1782)
                     /locus_tag="PKH42_09655"
     CDS             complement(1273..1782)
                     /locus_tag="PKH42_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOA20740.1"
                     /translation="MKKIAILIEDLFNERELIYPYYRLLEAGYEVHLVGPKRDAVYTS
                     KSGLTEKSTHASNEISAEDYDGVVIPGGFSPDFMRRTRATVDFVREMDKLNKPVAAIC
                     HGGWMLASACNLKGKKVTSFFSIKDDMVHAGAEYVDEEVVVSGNIITSRNPNDLPVFL
                     KTIIEKLNQ"
     gene            complement(1982..>3691)
                     /locus_tag="PKH42_09660"
     CDS             complement(1982..>3691)
                     /locus_tag="PKH42_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOA20741.1"
                     /translation="DITERKQMEDALIESERSKAVLLSNLPGMAYRCNYDRDWTMQFV
                     SEGCYELTGYKSGSLINNRDLSFNDLIAPEYQDILWNVWKKILIKKKPFKYEYEIVSA
                     TGQRKWVLELGQGIYDEQGTVVALEGIIIDISERKEHENKLKYISEHYELTGLYNQRY
                     FEELLVKESKLKRCDKKAIILINLKKINSVSLTYGYNFSRRLIVELAEKLNMLNNNNI
                     ILFQISLDRFAFYIKKYKNEFELIEFCSKIFCKIKEVQILNNLGCRIGIYEIEEDNWD
                     LESIIKNVSVAADSVNNKSIFNYCFYNDELKAKVTRENDIRDELFKEITDDNSKTIYL
                     QYQPILNLKTGKISGFEALARFKSQKLGLVQPSEFIPIAEEMQLIVPIGLRVINKACN
                     FIKKLETAGFDNINVSVNVSVIELLRDEFIDALKTLIKNAEINAKNLGIEVTESIIMD
                     NFEIINEKLYKLREMGIEVSIDDFGTGYSSLSRESELNVCNLKIDKYFIDKINEQNTD
                     KIIAGDIISMAHKLGHFVVAEGVENKEQYEYLLKNNCDFIQGYYIGKPLDENGVIKLL
                     NNN"
BASE COUNT         1183 a          689 c          559 g         1260 t
ORIGIN      
        1 cgatggaacc cacagtaaaa taaactttga cggaactgaa accgcttcaa aaaaaccttt
       61 taaggagcgt gccgaagtat tagaggggcc cggtataaac ttattagatg atggcagatg
      121 cgcttatgcc aggttttgtc acagaaacga cggagatgcc tgggagctga cagaacaatc
      181 tgatgatgag catttaagag aagaagcaat cattgcagct aaggaatgcc ctgcaggaag
      241 attgcttgca gtgaccaaaa acaaggagga tattgagcct gaattagagc cttccataga
      301 tatacttcaa gacgaggaat taaatgtcag cggtcctatt tacgttaaag gttatatacc
      361 catagaatcg gctgacgggg atgtatacga agtaagaaac agacaaacac tttgccgatg
      421 cggaaaatcg aaaattaagc cattttgtga ttctgcccac gttaaatcaa attttaggga
      481 ttatgagtcg gacgataaaa atcattagag attctttcca tttcattaac aaaaataaag
      541 tttggacata taatatcgta gtcgatagtt tgtactatta actgcgatat ttttggtaaa
      601 gggtgaaaat ttatttatgg actgtccgtt ttgttgtttt ttaagaaatt atataggaca
      661 atatataaga attggcgtag tttgcaattg tgatactaat gaccatattt acagggatgg
      721 atatatcaca aaaaaatcat tttacaacaa tgcaataagg ttgtatgatt caccgacaag
      781 cggcgaagta atatttactg cctgctgcga tagtattttt gaaatctata ttttcgatac
      841 tattacgccg actgcagatg caaaaccgag tgatattaca gacatagcca caatggtgga
      901 acaaaggcgc aatttattgg aatctgtact aggtaacttg cagaaggagg agacggcaaa
      961 tgaataagta taaagaagat tgtgatattt gtaaattatt acgtgatttt ggagatagac
     1021 tagtgtttat caaattcaaa tgcccgactt atttcggtac tgtaggcaga atattagata
     1081 tcagagacaa tcttgtaata ttggaatata ttaatggcgg cataagcgta acttgctgcg
     1141 acaatatttg ttatgtcgta ccggtagcag aataagaata gaaaataaaa gcgttaaaat
     1201 aattccggca gaatatctgc cggagttcat ttttagtaat caaccctatt atgaatacaa
     1261 aagaaaaggt ttttactgat tcaacttttc aatgattgtc tttaaaaata caggcaggtc
     1321 attagggttt cttgaagtaa taatattgcc tgaaactaca acctcttcat ctacatattc
     1381 tgctcctgca tgaaccatat catcttttat tgagaaaaat gaagtaacct ttttaccttt
     1441 taaattacat gctgatgcaa gcatccatcc cccgtgacaa atagcagcaa caggcttatt
     1501 tagcttatcc atttccctta caaaatcaac cgttgccctt gttcttctca taaaatcggg
     1561 tgaaaatccc cctggaataa ctaccccatc atagtcctct gcactaattt cattagaagc
     1621 atgcgtactt ttttccgtaa gccctgattt cgaagtataa acggcatccc ttttcggacc
     1681 taccaaatga acttcatacc ctgcttccag caacctgtaa taaggatata taagctctct
     1741 ttcattaaat aaatcttcta ttaatattgc tatttttttc atttattctc cctcctaaat
     1801 gtacttaata tatacttacc caaattgttg attttaaatc tttgataatc caatgatttt
     1861 accgacctgg tgccaggcac cagtgcctca aatggtgcct ggcaccgtaa atacgaggtt
     1921 aaaagtataa gcaataataa tcggagacta cataatattc aataactatg cattctccga
     1981 ttcagttatt attaagcaat ttaattactc cattctcatc aaggggttta ccgatataat
     2041 atccttgaat aaagtcgcaa ttatttttga gaagatattc atactgctct ttattttcaa
     2101 caccttcagc caccacgaag tgtcccagct tatgagccat tgatataata tcacctgcaa
     2161 taatcttatc agtattttgc tcatttattt tatcgatgaa gtatttatcg attttcaaat
     2221 tacatacatt tagttcgctt tcacgggata atgaagaata tcctgttccg aaatcatcaa
     2281 ttgaaacctc tatccccatt tcccttaatt tatacagctt ctcgttaata atttcgaaat
     2341 tatccataat aatagattcg gttacttcta tacctaaatt tttcgcattg atttctgcat
     2401 ttttaatcaa tgttttcaaa gcatcaatga attcatctct tagcaattct attacagata
     2461 catttacaga tacatttata ttgtcaaaac cggccgtctc caactttttt ataaaattgc
     2521 atgccttatt tataaccctt aatccgattg gtacaataag ctgcatctcc tcagctatag
     2581 gtatgaattc ggaaggttgt acaagaccaa gtttttggct tttaaagcgg gctaaagctt
     2641 caaacccgct tattttccct gtttttagat tcaaaatcgg ttgatactga agataaattg
     2701 ttttagaatt atcatccgtt atttctttaa ataattcatc ccttatatcg ttttctctcg
     2761 ttactttagc ctttaattca tcattgtaaa aacaataatt aaatatgctt ttattattaa
     2821 cgctgtcagc cgcaacagaa acatttttta ttatactttc caaatcccaa ttatcctctt
     2881 caatttcata tattcctatt ctgcagccta aattatttaa tatttgcact tccttaattt
     2941 tgcagaaaat tttactgcaa aattcgatta attcaaattc gtttttatat tttttaatat
     3001 aaaaagcaaa tctgtccaag gaaatttgaa acaatataat attattatta ttgagcatat
     3061 tcagtttttc tgctaattct acgataagtc ttctgctgaa attatatccg taagttaaac
     3121 tcaccgagtt tatttttttt aaatttatta gtataattgc ttttttatca catcttttta
     3181 attttgattc tttaaccaag agctcttcaa aatacctttg attatataaa cccgttagtt
     3241 cataatgctc acttatatat ttcagtttgt tttcgtgttc ttttctttct gaaatatcaa
     3301 taataattcc ttcaagtgct acaacggtac cctgctcatc ataaataccc tggcctaatt
     3361 ccaaaaccca ttttctttgt ccggtggcag atacaatttc atattcatac ttaaaaggtt
     3421 ttttcttgat aagaattttt ttccacacat tccacaatat atcttggtat tccggagcaa
     3481 tcaaatcatt aaaagacaag tccctattat tgattaagct gccagattta tatcccgtca
     3541 attcataaca accctcagaa acaaactgca ttgtccaatc cctatcatag ttgcagcggt
     3601 aagccatacc gggcaaattg gacagaagta ctgccttgct tcgctcactc tctatcaaag
     3661 catcttccat ttgctttctt tcggtaatat c
//
LOCUS       DAOPUE010000160         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_25911_length_3638_cov_11.789841, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000160 DAOPUE010000000
VERSION     DAOPUE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25911_length_3638_cov_11.789841"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1614
                     /locus_tag="PKH42_09665"
     CDS             226..1614
                     /locus_tag="PKH42_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20742.1"
                     /translation="MKRVIKTIISAALILSSAAAYANSGPVYMPEYPSSEIMAVDKNS
                     PIEVINENLIFDFSGSAGRYLDSYECMVTAEYEMANPESEDLSVQMAFPFIERIGGLS
                     EDNIKIRSDNEELDFEIYLGETVETNKADTENENYFQFEKIIGSISNDIYSAENFSQD
                     EAGNLYSIEINTDNDIRIAADLNYDYEKTKILAGEFNGFSREGGKTRLTAWCRDSEVL
                     YIYVLGHDVEFSISGYTDGGLSDKTDEFTYEISKKEIDVKTYLLDYIKNNPYISFENI
                     SDIQLYNVFAEAMDEQFTNNLGFAALDELLNYGSTNRMITLVYNVEFPKNSQKTVSVS
                     YKTSGTMDRRNTKEPLYTYDYILNPAENWKDFKNLNIKIFPPEEAPYIVDSSIELTKE
                     GNIYTAYLETLPQDDLQFTIYGKEKVTFMDKIEGEIDKKAGYALLFAPFILGLIIIVF
                     IIYKLLKRSAAK"
     gene            complement(1698..3515)
                     /locus_tag="PKH42_09670"
     CDS             complement(1698..3515)
                     /locus_tag="PKH42_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOA20743.1"
                     /translation="MKRSKRVLSLLLVLAMLFSMAGCQSQPAAQPPAEEPPAADAMYT
                     PGTYTGKGAGINGEIEVEVTVAENEITNIKVLSHNETPGVSDLALSDVPANIVEHQSL
                     GIDAIAGATYSSEGIIEAAANALSQAGADIDALKAKVVETEKGNLITTEKEVDVVVIG
                     GGGAGLAAAVSAHENGASVLLLEKMPKVGGNTIISGAAYNCVDPIRQAAAGIEDSVDK
                     HFQQTYEGGDKVGNPELIRVLVENAYPTLQWLEGLGMEFTDYVFTVLGGLWPRAHRPA
                     MPLGTGYIATYMNYIENNSSDMEVMLNTQAKHILLEDGKAVGVSAEGPDGPVIVRANK
                     GVVIATGGFSKNVELRNKYNKTWGDLTNALSTNHPGATGDGIIMAEEIGANFVGMEYI
                     QLLPLGDPETGSLAGNIEQAVENRIFVNKDGNRFVDEGGRRDDMTKALFEQRDEFLWI
                     ILDSHNYPTGDTKNNFNETIDELVAAGRAYKADTLEDLAAQIGVNADNLVAAVEDFNA
                     HVESKEPDEFGRTLYQWKMDTPPYYAGARKPTVHHTMGGIEINTEAQVIDTQGNIIPG
                     LYAAGEVTGGIHGSNRLGGNALADINTFGRIAGRNAATK"
BASE COUNT         1098 a          710 c          652 g         1178 t
ORIGIN      
        1 atcatattct aatttgtaca aagtgttacg tctgtccaaa aatatttttg ggacaccggg
       61 tggtatgctg gtgacggtcc ttttattgtc ccgtcccaac tgtctcatcc acaatgtcca
      121 tccagtaaat ggaacatttt cttttgtata ttcgtcatat atgatacaat taatgtggaa
      181 agcgggacaa aaaatatgag tacccgctgt cccgggaggt atatagtgaa aagagtaatt
      241 aaaacaatca tatctgcagc attgattctt tcatctgcag cagcctatgc aaattcagga
      301 cctgtatata tgcctgaata tccgtcttcg gaaataatgg cagttgataa gaattctccc
      361 attgaagtaa taaacgaaaa ccttattttt gatttttccg gcagtgccgg cagatatctg
      421 gattcatatg aatgcatggt tactgctgaa tatgagatgg ccaatcctga aagtgaggac
      481 ttgtcagttc aaatggcttt tccctttatt gaaagaatag gcggactatc agaggataat
      541 attaaaatta ggtctgataa tgaagagtta gactttgaaa tttatttggg ggaaacggtt
      601 gaaaccaata aagcagacac ggaaaacgaa aattattttc aatttgaaaa aatcattggg
      661 tctataagca atgatattta tagtgctgaa aatttttctc aagatgaagc aggtaacctg
      721 tattccatag aaattaatac tgataatgac attagaattg cagcagacct taattatgat
      781 tatgaaaaaa caaaaatact tgcaggagaa ttcaacggat tttcccgtga aggagggaaa
      841 acacgattaa ccgcctggtg ccgggattcg gaagtcctgt acatttatgt actggggcat
      901 gatgtggaat tcagtatttc aggatataca gacggaggtc tttcggataa aacagatgaa
      961 tttacatacg aaatatctaa aaaggaaatt gatgtaaaaa cctatttgct tgattatata
     1021 aaaaataatc cttatattag ttttgaaaat atttcagata ttcaactgta taatgtgttt
     1081 gcagaggcaa tggatgaaca gtttacaaat aatttaggat ttgccgcatt ggatgaatta
     1141 ttgaattacg gaagcacaaa cagaatgata acgttggttt acaatgttga atttccaaag
     1201 aattcacaaa aaacagttag cgtaagctac aaaacttcag gaaccatgga caggcgaaat
     1261 acaaaggaac ctttgtatac ttatgattat attttaaatc ctgcagaaaa ctggaaggat
     1321 tttaaaaact taaacataaa gatttttccg ccggaggaag caccatacat tgttgacagc
     1381 agcatagaat tgacaaaaga gggcaatatt tatacggcat accttgaaac cctccctcaa
     1441 gatgatttgc agtttacaat atacggtaaa gaaaaagtca cttttatgga taagatagaa
     1501 ggggaaatag acaaaaaggc aggatatgct ttattgtttg caccatttat attaggatta
     1561 ataataattg ttttcattat ctataaactg ttaaaaaggt ctgcagcaaa ataatcggga
     1621 gcttacttta aaataactct tatataaaaa tggaacagca gggtattaac ttctgctgtt
     1681 ccataatttt ttattgctta tttggttgct gcatttctcc cggcaattct accgaatgta
     1741 ttgatgtcag ctaatgcatt tccgccaaga cggttagatc cgtggatgcc gcctgtaact
     1801 tcaccggctg catataatcc aggaataatg tttccttgtg tatctataac ttgagcttct
     1861 gtattaattt caattccgcc cattgtgtgg tgaacggttg gtttccttgc tcctgcataa
     1921 tatggaggag tgtccatttt ccactgataa agggttcttc caaactcgtc aggttctttg
     1981 ctttcaacgt gagcattgaa atcttcaaca gcagcaacta aattatctgc gttaactccg
     2041 atttgtgcag ccaaatcttc cagtgtatct gctttataag ctcttcctgc tgctactaat
     2101 tcatcgatag tttcattgaa gttattcttc gtatctccag taggataatt atgactgtca
     2161 agtattatcc acaaaaattc atctctttgt tcgaatagtg cttttgtcat atcatctctt
     2221 cggccgcctt catcaacaaa tctatttcca tctttgttta caaatatacg gttttcaaca
     2281 gcctgttcaa tattgcctgc caagcttcct gtttcagggt cacccaatgg taatagctga
     2341 atatattcca tgcctacaaa gttagcacca atttcttctg ccattattat tccatcaccg
     2401 gttgctccag gatggttagt agataaagca tttgttaaat caccccaagt tttgttgtat
     2461 ttgtttctca attcaacatt tttgctgaat ccgcctgttg caataacaac acccttattt
     2521 gctcttacaa ttaccggacc gtccggacct tctgcactga cgccgacagc tttgccgtct
     2581 tccagtaaaa tatgtttagc ttgtgtgtta agcatcactt ccatatctga gctgttattt
     2641 tctatgtagt tcatgtatgt ggcaatatat cctgttccta aaggcattgc aggtctatgg
     2701 gctcttggcc acaatcctcc aagaactgtg aatacataat ctgtaaattc cattcccagg
     2761 ccttccagcc actgtaatgt ggggtaagca ttttcaacta aaaccctgat tagttccgga
     2821 tttccaacct tatctccacc ttcataggtt tgctggaaat gcttgtctac agagtcctca
     2881 attcctgccg cggcttgtct tatagggtca acacagttat atgccgctcc tgaaattatt
     2941 gtattgcctc caaccttagg catcttttcc aataataata cagatgctcc attttcatga
     3001 gcagatactg cagctgccag tccggcacca ccgccgccta taactacaac gtctacttct
     3061 ttttctgtag ttattaaatt acctttctct gtttcaacta cttttgcctt taatgcatca
     3121 atatctgcac ctgcttgtga taaagcatta gctgctgctt caataatacc ttcgcttgaa
     3181 tatgtagcac ctgctattgc atcaatacct aaactttgat gttcaacaat atttgcagga
     3241 acatctgaca atgcaagatc acttacacct ggagtctcgt tgtgtgagag cactttaatg
     3301 tttgttattt cattttcagc tacagttact tcaacttcga tttcaccgtt aatacctgca
     3361 ccctttccgg tataagtacc aggagtgtac atagcatcag ctgccggcgg ctcttcggcc
     3421 ggtggctgtg cagccggttg ggattggcat ccagccatag aaaacaacat ggctaatact
     3481 aacaataatg ataatactct tttgcttctt ttcatgaaat cctcctattt tctttttgtt
     3541 gtatatatat cattatatta tatgcaaacg ttttgtcaac atttgacaaa acaacaacaa
     3601 aatgtgctga taaaactaaa tgatataaac tatgcaaa
//
LOCUS       DAOPUE010000161         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_27501_length_3485_cov_9.283090, whole genome shotgun sequence.
ACCESSION   DAOPUE010000161 DAOPUE010000000
VERSION     DAOPUE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27501_length_3485_cov_9.283090"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="PKH42_09675"
     CDS             complement(<1..140)
                     /locus_tag="PKH42_09675"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOA20744.1"
                     /translation="MTIIELFYENKNDDLAVPMAKYMKNKFPFLGIKTPERKELSKDF
                     LK"
     gene            complement(159..1337)
                     /locus_tag="PKH42_09680"
     CDS             complement(159..1337)
                     /locus_tag="PKH42_09680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOA20745.1"
                     /translation="MKRVIFLVDMNAFYISCEKTRHPEIEGKPAAVAGDPVNRTGIIL
                     TANYEARKYGVKTTMILREAFRLCPDLIIIPPDHRFYEKKSQEVMQILSSYTPIVEQN
                     SIDEAWLDMTGCERLFGQPLESARSIMDRIKNELNLWCSIGISENKFLAKMASEMKKP
                     LGITELWKKDIEEKLWPLPVDYMYGVGKQTAKKLNSMGIYTIKDLALTKREDIYHKLG
                     KIGVELVLLANGKDDTPVNVNREDIKSIGRSTTLPNDITDIEDAKAILMELSDDIGMT
                     ARESGKKGNTVQITIKYSTFNTITRQMTISPTCNIKDIYAAGVKLLERNWSNEPVRLL
                     GISLSGFQNESEQISLFQLDKNEAKGDEKIDNLEDTILKIRKKYGADIIKPGIPHKKE
                     "
     gene            complement(1374..2420)
                     /locus_tag="PKH42_09685"
     CDS             complement(1374..2420)
                     /locus_tag="PKH42_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20746.1"
                     /translation="MNIKYIQELLSRINEVRIINKKVDIEGVVCNIAGIVRYGQKLRL
                     IILEYDEVYRQKVEEMELSEPCELRQQETNRSILMGRGRIEAAQPFRPIKSVFIDEME
                     FEINVTENRLLNVKDGESVLFLSELLRNGWNPEGVDYQSIDMLFITSIEFAGDFDKIP
                     EFDDNVKLHFTMNMDMVTYLVEQPVTLTVNGEYPEKLWFNNKEDNKEHWAQINRVYLL
                     NMWAEMEKSFSDKRLLEHMTKEQIEEAKRNIEKSFVDVCPKGMYYPVIEYESEDDISL
                     EFHTKKFLDSKPVHHGSGSIGFIISPDKPTGILGKKLKSAIIQEPVTENTEIIEAELF
                     QYHRTITPEDVILC"
     gene            complement(2469..2825)
                     /locus_tag="PKH42_09690"
     CDS             complement(2469..2825)
                     /locus_tag="PKH42_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2793..2795),aa:Sec)
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOA20747.1"
                     /translation="MNYTFICYSKUGTCKKAKDWLIKNDIDFTERSIKEENPNESELS
                     DWIKKGKCSIKRFFNTSGTLYKDLNLKEKLENMSDEEQIKLLSTYGMLVKRPILVGGD
                     IVLVGFKENEWREKLL"
     gene            complement(2858..3484)
                     /locus_tag="PKH42_09695"
     CDS             complement(2858..3484)
                     /locus_tag="PKH42_09695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOA20748.1"
                     /translation="MRREEQTDYERVEEITRKAFWNLYTPGCNEHYLVHVMRFHKDFL
                     PELDFVIEVDNQIIGNIMYTKARLIDEAGEEKNILTFGPVSILPEYQRRGYGKKLLEY
                     SFEQAAALGYDVIVIFGNPGNYVSRGFKSCKKFNVCLEDGTYPTAMLVKELKPNVLDG
                     RKWVYYQSPVYEIDEQEALCFDDALEKMEKKYLPCQEEFYILSNSIIK"
BASE COUNT          970 a          789 c          460 g         1266 t
ORIGIN      
        1 tccttcaaaa aatcctttga cagctctttt ctttcaggag ttttaatccc taaaaaggga
       61 aatttatttt tcatatattt agccatagga acagctaagt catcattttt attttcataa
      121 aacaattcaa taatcgtcat aataacacta cctttctact attccttctt atggggtatt
      181 ccgggcttta tgatatcagc tccgtatttt tttcgaattt ttagaatagt atcttctaaa
      241 ttatcaattt tttcatcgcc ttttgcttca tttttatcca attgaaacaa cgatatctgc
      301 tcgctctcgt tttgaaaacc gcttaaactt atccccaata aacgcacagg ctcattgctc
      361 caatttcttt caagcagctt tacacctgca gcataaatat ccttaatatt gcaggttgga
      421 cttattgtca tttgccttgt tatcgtatta aaagttgaat atttaattgt tatttggact
      481 gtattaccct ttttaccgct ttctcttgca gtcataccta tgtcatcgga caattccatc
      541 aatattgctt tggcatcttc aatatctgta atatcattag gcagggtagt tgaccttcct
      601 atggatttaa tgtcctccct gttcacattt actggagtat catctttgcc gttagccagc
      661 aaaaccaatt caacccctat tttccctaat ttatgataaa tatcttccct ctttgtcaaa
      721 gctaaatcct taattgtata aatacccatg ctattaagct ttttagctgt ctgcttcccc
      781 actccgtaca tatagtcaac cggtaaaggc cacaattttt cttctatatc ctttttccac
      841 agctcagtga tacctagcgg ctttttcatt tccgaagcca tttttgccaa aaacttattt
      901 tcagatattc cgattgagca ccacaaattg agttcattct ttattctgtc cattatactt
      961 cttgcagatt ctaagggctg accaaacaaa cgctcacacc ctgtcatatc cagccacgct
     1021 tcgtcaatgc tgttttgctc aactatggga gtgtagcttg acaaaatttg cataacttct
     1081 tgggacttct tctcataaaa tctatggtca ggaggaatta taatcaaatc cgggcatagc
     1141 cgaaaagcct cccttaaaat catggttgtt tttacaccat attttcttgc ttcataattt
     1201 gcagtaagta ttattcccgt acggttgacg gggtcccccg caaccgctgc tggtttgcct
     1261 tcaatttcag gatggcgagt tttttcgcag ctgatataaa aggcattcat gtctaccaaa
     1321 aatattaccc ttttcatata tataaccttc acattcattg gtgcctggca ccactaacac
     1381 aaaattacat cctccggagt tattgtcctg tgatactgaa ataattctgc ttcaataatc
     1441 tcagtgtttt ctgtaacagg ctcctgtata attgcagatt ttaatttctt tcctaagatt
     1501 ccggttggct tatcagggct gattataaat ccaatgctgc cgcttccgtg atgaacaggc
     1561 tttgaatcaa gaaatttttt tgtatgaaat tcaagcgaga tatcgtcctc actttcgtat
     1621 tcgataacag gataatacat ccccttagga caaacatcaa caaaactttt ttcaatattc
     1681 ctttttgcct cttctatctg ctccttagtc atatgctcta atagtctttt atctgagaag
     1741 cttttttcca tttcagccca catgttcaat aaataaaccc tgtttatttg agcccaatgc
     1801 tctttattgt cttccttgtt attaaaccat aacttttcgg gatattctcc atttacggta
     1861 agtgtaacag gttgttcaac caaatatgta accatgtcca tattcatggt aaaatgcagc
     1921 ttaacattat cgtcgaattc cggtatctta tcaaaatcac ccgcaaattc aatactggtt
     1981 ataaacaaca tatctatact ttgataatca actccctcag gattccagcc gtttctcagt
     2041 aattcagaca gaaataacac actctctccg tctttaacat ttaagaggcg gttctccgta
     2101 acatttattt caaattccat ttcatcaata aatacacttt ttataggtct aaaaggctgt
     2161 gcagcttcta ttctacccct gcccattagt attgaccggt tcgtctcttg ttgtctaagc
     2221 tcacaaggct ctgacagttc catttcttca actttttgac gatacacctc atcatattcc
     2281 aatataataa gacgcaattt ttgtccgtat cgcactatcc ctgcaatatt acagacaaca
     2341 ccttcaatat ctaccttttt gttgattatt cttacttcat ttattcttga taaaagttct
     2401 tgtatatatt taatattcat gaattcacct atgtttaata aaatcgcact actttgatat
     2461 ttcaattgtc aaagaagttt ttccctccac tcattttctt taaatccaac taatacaata
     2521 tcccctccaa caagtatggg tctttttacc agcataccat aggttgacaa cagtttgatt
     2581 tgttcttcat cagacatgtt ttctaatttt tcttttaggt ttaaatcctt atacagcgtg
     2641 ccactagtat taaaaaatct ctttatagag catttgcctt ttttaatcca atcactcaac
     2701 tcgctctcgt ttggattttc ctcctttatg gatcgctccg taaaatcaat atcatttttt
     2761 attaaccaat ccttggcctt tttacatgtg cctcatttcg aataacaaat aaatgtatag
     2821 ttcatttcat cctcctcggt ttattttaaa aagaagttta ctttataata gaattactga
     2881 gaatatagaa ttcttcctgg catggcaggt attttttctc cattttttcc aaggcatcgt
     2941 caaaacacag agcttcttgt tcatctatct catatacggg actctgataa taaacccact
     3001 ttcttccgtc caatacatta ggtttgagtt cttttaccaa cattgcagtc ggatatgtcc
     3061 catcctcaag acagacatta aatttcttac agctcttaaa cccgcggcta acataattac
     3121 ccggatttcc aaatattaca attacatcat aaccaagagc agccgcttgt tcaaaagaat
     3181 actccaataa ttttttccca tacccccttc tctggtattc cggcaggatg ctgaccggac
     3241 caaaagtaag gatatttttt tcttcccctg cctcatcaat aagtcttgct ttcgtataca
     3301 ttatattacc gataatctga ttatcaactt caataacaaa atccaattcc ggcagaaaat
     3361 ccttgtggaa acgcataaca tgaaccaagt agtgttcatt gcacccggga gtatataaat
     3421 tccaaaaagc tttccttgtg atttcttcta ccctctcata atctgtttgt tcttctcttc
     3481 ttatt
//
LOCUS       DAOPUE010000162         3474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_27627_length_3474_cov_9.644340, whole genome shotgun sequence.
ACCESSION   DAOPUE010000162 DAOPUE010000000
VERSION     DAOPUE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27627_length_3474_cov_9.644340"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..786)
                     /locus_tag="PKH42_09700"
     CDS             complement(220..786)
                     /locus_tag="PKH42_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20749.1"
                     /translation="MDSDINYIIHKSLKGDKIYQEILLKKLNPLIFNNIYKYYKASNP
                     LVEDLLHEGYIIILQSLKDYDAKRNVHFLQYVKIRLFYFYKNYYKKSIKNNTLSVEYL
                     NETGKEFKSDSPAQISTVILKEQKAALYKCMNELSEKEQKVLNLFYFQRFSIQEISEE
                     LSLEYRSVINIKSKAVKKLRSKCYLLME"
     gene            932..1399
                     /locus_tag="PKH42_09705"
     CDS             932..1399
                     /locus_tag="PKH42_09705"
                     /inference="COORDINATES: protein motif:HMM:NF018087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HOA20750.1"
                     /translation="MKNEIKFNEIEAMLPVYVKDKGNCTEIITKEASYIKTATIETCT
                     KKLADYYNISLYHNRINYGQELGITNKVPLVVNENLIYIYVNVRKPMFNHDAAFGLFD
                     VNSIENIYDDDGKAVIEMKSGKMIKTRQSVKSLKKSILNARIAKEIYKEKHNK"
     gene            1489..1761
                     /gene="yabP"
                     /locus_tag="PKH42_09710"
     CDS             1489..1761
                     /gene="yabP"
                     /locus_tag="PKH42_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOA20751.1"
                     /translation="MDEKIMQSLVLENKEILNVTGVEGVDNFNDETVVLITTKGRLTI
                     KGENLAISKLNVDEGKLMVKGIINSLVFSEYEGQREKVSLVKRLFK"
     gene            1763..2320
                     /locus_tag="PKH42_09715"
     CDS             1763..2320
                     /locus_tag="PKH42_09715"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOA20752.1"
                     /translation="MDYLPYSQEYMLAVSIMAGMFLGFIWDIYRLIRHYGKFKTTGTV
                     IGDIIYWIVSITISTRLILDISYGNVRFFILLGFLAGALLYFYGISSYVLKLFIFIID
                     SIIKVIKTVIHLLIDPIKFIIRKIEIFLYPLKLKYHKVNEKLKKRYKFLKYKLKKVFK
                     NKKMIYNRKKRKRQLKKRKKRRLSP"
     gene            2375..2833
                     /locus_tag="PKH42_09720"
     CDS             2375..2833
                     /locus_tag="PKH42_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOA20753.1"
                     /translation="MNEDQRIIELKKKINHYDFREKEREIKEQKRIKKLAAPIKKKRR
                     FNVINFLFLIFVIYFAFTAFNQYEMLLDLNGQIKEKEAIKAEAEKEALELKSDVEKLN
                     EEETLMEIIEKIARDQYKMVKPNETIYIDKNKNDNKLIQGIGSQKDLINE"
     gene            2911..>3474
                     /locus_tag="PKH42_09725"
     CDS             2911..>3474
                     /locus_tag="PKH42_09725"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20754.1"
                     /translation="MIGRTQDEKYDLIITKTLKKELKHIKENWLLLLVCFFVSRQNII
                     NEIFPFAIVVLSSYCHIKGPWISILFVCIAAILSVRFDFVYIIMLTAIFGYYFNFRNE
                     GKKSILLISGYSAAVLFFSKTAILVSEGFNTNEFMLNVFETMFIFSAIILINEGFKSI
                     KKIIKEKSEKNQRENKKKEVKANNNNAE"
BASE COUNT         1327 a          463 c          593 g         1091 t
ORIGIN      
        1 attgctatga ataagctacg tgtcattctg agagctttag ctcgaagaat ctcatggagg
       61 gttgtcattc tgaatgcagt gaagaatctc atgaattttt gtcattctga acgcagtgaa
      121 gaatctcatg cagggttgtc attctgaaca cagtgaagaa tctcatgcag ggttgtcatt
      181 ctgaacacag tgaagaatct cataaatatc gctcaattat tattccatta gcaagtaaca
      241 tttgcttctc agttttttaa cagctttgct tttaatgttt ataactgagc gatattctaa
      301 acttaattct tcagaaattt cctgtattga aaatctttga aagtaaaaca agtttaaaac
      361 tttttgttct ttttcggaca gctcattcat gcatttatac agtgcagctt tctgttcttt
      421 gagtattact gttgatattt gagccggact atcgctttta aattcctttc ctgtttcatt
      481 taaatattca acgcttaatg tattgttttt aatacttttc ttgtaatagt ttttataaaa
      541 ataaaatagc cttattttaa catactgtag aaagtgaaca tttctttttg catcataatc
      601 ctttaatgat tgcagaatta tgatataacc ttcatgcagc aaatcttcga caaggggatt
      661 cgaagcctta taatatttat aaatattatt aaaaatgagc ggatttagct ttttgagcaa
      721 tatttcctga taaatcttgt ccccttttaa agatttatga ataatatagt taatgtcaga
      781 gtccattttc atcacccggc ttattatacc ggtttaataa aattacatgt aaatggtaat
      841 gaccaaaaac tgtctttgaa attgataaaa ttccgaaaaa cccgaaatgt taaaatatca
      901 ataaatttta ttctgaagga aatgaaaata tatgaaaaat gaaataaaat tcaatgaaat
      961 agaggcaatg ctgccagtct acgtgaaaga caaaggaaac tgcacggaaa tcataacaaa
     1021 agaggcatct tatataaaaa ctgcaacaat tgaaacatgc acaaagaaat tagcagatta
     1081 ttataatata agcttgtatc acaatcgtat aaattacgga caagagctgg gaattaccaa
     1141 taaggtgcca ctggttgtaa atgaaaatct tatttatata tacgtcaatg ttagaaaacc
     1201 tatgtttaat catgatgctg ccttcggatt atttgatgta aattctatag aaaatattta
     1261 tgacgatgac ggtaaggcgg taatagaaat gaagtcaggt aaaatgatta aaacgagaca
     1321 aagtgttaaa tctttgaaaa aaagtattct taatgcaaga attgcaaagg aaatatacaa
     1381 agaaaaacat aacaaatgag actgcgagat gacagtaccc ccgtcccctt gtcatctgtc
     1441 ataaataaaa ttccatggac atataatata tttgaggggg gaatgttcat ggatgaaaaa
     1501 attatgcaaa gtttagtact tgaaaataaa gaaatattaa atgtcaccgg tgttgaagga
     1561 gtagataact tcaatgacga aacagttgtg cttatcacta caaagggcag acttaccata
     1621 aagggagaaa atttagccat aagcaagcta aatgttgatg aaggcaaact tatggtaaaa
     1681 ggtataataa attctctggt tttctctgaa tatgaaggac agagagaaaa agtgagctta
     1741 gtaaagagac tgttcaaata atatggacta tttaccttat tctcaggaat acatgcttgc
     1801 agtttcaata atggcaggta tgtttcttgg ttttatatgg gatatttaca ggcttataag
     1861 gcattatggc aagtttaaaa caacaggaac tgttataggt gacattatat attggatagt
     1921 cagcataact ataagcaccc ggttaatctt agacatcagt tacggcaatg tcagattttt
     1981 tattcttttg ggatttttag cgggtgctct cttgtatttc tacggaatca gcagttatgt
     2041 tttaaagctt ttcatattta ttattgactc tattataaaa gttataaaaa cggttataca
     2101 tcttttaata gacccgatta agtttataat tagaaaaata gagatttttt tgtatccttt
     2161 gaagctaaaa taccataaag ttaacgaaaa actcaaaaaa agatataaat tcttgaaata
     2221 taaattaaaa aaggttttca aaaataaaaa aatgatatat aataggaaaa aacggaagag
     2281 gcaattaaaa aaacgtaaaa aaagaagatt gtcgccctga gcctaagata aggttgccct
     2341 aacctgaaat tacaaaagaa aggagtagtc tcagatgaac gaagaccaaa gaatcataga
     2401 attgaagaaa aagattaatc attatgattt tcgtgaaaaa gaacgagaaa taaaagaaca
     2461 aaagagaatt aaaaagctgg ctgctccaat aaagaaaaaa agaagattca atgtaattaa
     2521 ttttctgttt ttaatatttg ttatatattt tgcattcact gcttttaatc aatatgaaat
     2581 gcttttggat ttgaacggcc agataaaaga aaaggaagcg ataaaggctg aagctgaaaa
     2641 ggaggcattg gagcttaaaa gcgacgtgga aaaattaaac gaagaagaaa ctctcatgga
     2701 aataattgag aaaatagcaa gggaccaata taagatggtt aaacccaatg aaacaatata
     2761 catagataaa aacaagaatg acaataagct tattcaaggg ataggctctc aaaaagactt
     2821 gataaatgaa taaattttta tgatttaagc atattttgac agtattttac aatcatattt
     2881 gataccatag cggaaacgag gggtgaaaat atgattggaa gaacacagga tgaaaaatat
     2941 gatttaatca tcactaagac attaaagaaa gaacttaaac acatcaaaga gaattggctg
     3001 ctgcttttgg tgtgtttttt tgtttcaagg cagaatataa tcaatgaaat atttcccttt
     3061 gccattgttg tattaagttc atactgtcac attaagggtc cttggatttc aatactgttt
     3121 gtgtgcatag cagcaatttt atcggtacgt tttgattttg tgtacatcat tatgctaacg
     3181 gccattttcg gatactattt taatttccgt aatgaaggca aaaaatcaat attattaata
     3241 tccggatatt ccgctgcagt attgtttttt tcaaaaacag ctatcttggt ttccgaaggg
     3301 ttcaatacca acgaatttat gcttaatgta tttgaaacaa tgtttatttt cagtgccatt
     3361 attctaataa acgaaggatt taaatccata aaaaaaataa taaaagaaaa atcagaaaaa
     3421 aatcaaagag aaaacaagaa aaaagaagtt aaagctaata ataataatgc agaa
//
LOCUS       DAOPUE010000163         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_27838_length_3454_cov_10.625772, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000163 DAOPUE010000000
VERSION     DAOPUE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27838_length_3454_cov_10.625772"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..331
                     /locus_tag="PKH42_09730"
     CDS             23..331
                     /locus_tag="PKH42_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOA20755.1"
                     /translation="MEKVYKYTITDEEIMENIFKDEKILMNHVIVPPGKVFPKHKTDA
                     IVYALIIRGELSVAIENNEIKTYRAGQLVSIPKGVSTELGNRGEETLELFVVKHEYDL
                     "
     gene            411..1535
                     /locus_tag="PKH42_09735"
     CDS             411..1535
                     /locus_tag="PKH42_09735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOA20756.1"
                     /translation="MNDKKKYKISLLGEFIDKSIESEYLIDSLRSSSKITSYTALVIG
                     FIMMLFLVNGYIVDDRTPYFLNIILIRMLVVIISVIIFTVAKKIKEYRNFIYLITFYQ
                     AVMVVYYIFVLTQYNPLSYFSVLGLMVITLAIYILPNKIIISQIISIILSILFFIYPI
                     KKIEGLEEYEFYKIIVYQIILLIYCNVNIYWTEFNKRKEFAANKELMDLSNKDPLTGI
                     YNRLKFDDDMNKWISFSERYGNPFSIILFDIDGFKDINDKYGHLAGDSVAIKIAETIS
                     KSIRDTDIFARWGGDEFVLLLPNTDVQEAENMAERMRECISNNLCDPISNITCSFGVA
                     AYEKNDTTQSLLNKADKLLLQAKASGKDRVVSMDSCTAQN"
     gene            1545..1919
                     /locus_tag="PKH42_09740"
     CDS             1545..1919
                     /locus_tag="PKH42_09740"
                     /inference="COORDINATES: protein motif:HMM:NF028342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOA20757.1"
                     /translation="MFFIAGIYPKREELDYYEPIMCSCCTKYGRFEAYVEYSVLSLFF
                     IPLFKFNKKYYARTTCCNSLYLITNKEKGLMMERGQGHNVFLKDTDITLIQKGICENT
                     CPNCGFNVSEEHKYCPNCGNKL"
     gene            complement(1990..2274)
                     /locus_tag="PKH42_09745"
     CDS             complement(1990..2274)
                     /locus_tag="PKH42_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20758.1"
                     /translation="MAFLNKIGKAIGEAAGTASEKAKDFAETTKLKNAISAEEKQINQ
                     YFLEIGKYVFEQERNNPDSPLAEVFSKITDSLRNIEELKRKITEIKLDNN"
     gene            2620..>3454
                     /locus_tag="PKH42_09750"
     CDS             2620..>3454
                     /locus_tag="PKH42_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20759.1"
                     /translation="MQQDKVIVMPFLSNRNIENAASKLNVAYKDAEENFPEDNHNDVY
                     LEISNKLSNLISNIHGESQLIELFAQGGKIPDKKYLTNGVNAIRQNCLKITKTINNLI
                     ELRKLEKKQTCLVVNSVNIVEIIDNIVINASKHIKQEIIFDTNIEEKYMSCDINKLQK
                     AILIILSVAVRYSNKKEILVNLNVFEDNINITVSFNNNSKLLNFFIQKMDNLTPDNLD
                     ELSLSFYLCKSLIALHEGSISVEGNSFETMFTVKLPCENTDSINYLFMRDNKNEYLAE
                     QI"
BASE COUNT         1242 a          431 c          599 g         1182 t
ORIGIN      
        1 ttctatgaat taggaggaat atatggaaaa ggtttataag tatacgataa cggacgaaga
       61 aataatggag aatattttta aagatgagaa aattcttatg aatcatgtta ttgttccacc
      121 cggtaaagtg tttccaaagc ataaaactga tgcgattgta tatgcactga ttattcgagg
      181 agagttgagc gtagcaatag aaaataatga gattaagacc tatagagcag gacagttagt
      241 cagtattcct aaaggtgtca gtactgaatt aggcaataga ggggaagaaa ctttagaatt
      301 atttgttgtt aaacatgaat atgaccttta aatgaaactc ttgcctggtc ggagtattat
      361 gagcagtagt ataaaggact tattaatatt ggttttgggg ttgtatgtgt atgaatgata
      421 agaagaagta taaaatatct ttgcttggtg aatttataga caagtctatt gaaagtgaat
      481 atctaatcga tagtttaaga agttcatcta aaattacatc gtataccgca cttgttattg
      541 gttttataat gatgctgttc cttgttaacg gttatattgt tgatgataga actccctatt
      601 ttttgaatat tatacttata aggatgctag tcgtcattat ttcagtgata atttttactg
      661 ttgcaaagaa aataaaagaa tatagaaatt ttatttattt aataactttt tatcaggcag
      721 ttatggttgt ttactacatt tttgtattga cacaatacaa tccgctcagc tatttcagcg
      781 tactgggttt aatggtcata acattagcaa tttatattct tcctaataaa attataattt
      841 cacaaattat ttctattatt ttaagcattt tattttttat ataccctatt aaaaagatag
      901 agggtcttga agaatatgaa ttttataaga ttattgtata tcaaattata ttgcttatct
      961 attgcaacgt aaatatttat tggaccgaat ttaataaaag aaaggaattc gcagccaaca
     1021 aagaactgat ggatttatct aataaggatc ctttaaccgg catttataac aggttaaagt
     1081 ttgatgatga tatgaataaa tggataagtt tttcggaaag gtatggcaat cccttttcta
     1141 taatattatt tgatatagat ggttttaaag atataaatga taaatatggg catttggcag
     1201 gtgacagcgt agcaataaaa attgctgaaa caattagtaa atcgattagg gatacagata
     1261 tttttgccag atggggcggg gatgaatttg tgcttcttct gccaaataca gatgttcagg
     1321 aagcagaaaa tatggctgaa cgaatgagag aatgtatcag taataacttg tgcgacccga
     1381 taagtaatat tacttgcagt tttggtgtag cagcttatga aaaaaatgat acgactcaat
     1441 cattgcttaa taaagcagat aaattacttt tacaagctaa ggcaagcggg aaggatagag
     1501 tcgtgagtat ggatagctgt actgctcaga attaggaggt ctttatgttt tttattgcag
     1561 gtatatatcc aaaaagagaa gaattagatt attatgaacc gataatgtgc agctgctgca
     1621 caaaatacgg tcgatttgaa gcatatgtgg aatatagtgt attaagttta ttttttatac
     1681 cattgtttaa atttaataaa aaatattatg caagaacgac ttgctgcaac agcttgtatt
     1741 taataacaaa caaagaaaaa ggtctaatga tggaaagagg acaaggacac aatgtttttt
     1801 tgaaagatac agacataacg ctaatccaaa aaggaatatg tgaaaacaca tgccccaact
     1861 gcggctttaa tgtgagcgaa gagcataaat attgcccaaa ctgcggaaat aaattataaa
     1921 aagggaaaac aacatctatt aattaattat aataaaggag cagtattgta tcaataccgc
     1981 tcctttcaat taattgttat caagctttat ttcagtaatt tttctcttta attcctcaat
     2041 atttcttaag gaatcggtaa ttttgctgaa aacttccgct aaaggactat ccggattatt
     2101 tctttcctgt tcaaaaacat attttccgat ttccaaaaaa tattggttga tttgtttttc
     2161 ctcagctgaa attgcatttt tgagtttggt tgtttcagca aaatcttttg ccttttcaga
     2221 agccgttccg gcagcttcac caattgcctt gcctattttg tttaagaatg ccatggttaa
     2281 cccctccaat caaatatatt gttatttata taccttatat ttcttttttt aaacatttat
     2341 gatgaaaact ttatatactt attttatact aatgtgaatt atttgcaatc accctgaggg
     2401 tgatttaaca gataacttgt agtgttgttg caaaatacga caaagcaagg attaagtcac
     2461 gtcaaaaata ttttttatta gcgccaaatt tcgacacatt tttacgattt agcaatttat
     2521 aatgattacg tttgtgacct tttttgattt tattatattt tttatgataa aaatctaaat
     2581 aatatgtatg gtaattaata gcggggggag gtgataatat tgcaacaaga caaagtaatt
     2641 gtaatgcctt ttttaagcaa cagaaatatc gaaaatgcag caagcaaatt gaatgtagca
     2701 tataaggatg cagaggaaaa ttttcctgaa gataatcata atgatgtata tttggaaatt
     2761 tcaaataaat taagcaatct tataagtaat attcatggag aaagtcagct tattgagctt
     2821 tttgcacaag gtggaaaaat tccggacaaa aaatatttga caaatggggt taatgcaata
     2881 cgacaaaatt gtttaaagat aacaaagact ataaacaatt taatcgaatt gagaaaactt
     2941 gaaaagaaac aaacttgttt agtggtaaac tctgtaaata ttgtcgaaat tattgataat
     3001 atagttatta acgcatcaaa gcatatcaaa caagaaataa tttttgatac taatattgaa
     3061 gaaaaatata tgtcatgtga tatcaataag cttcaaaaag caattctaat tattttatcc
     3121 gtagccgtca ggtactccaa caagaaggaa atattagtta atctgaatgt ttttgaagat
     3181 aatattaata ttactgtttc atttaataat aatagtaagt tattgaactt ctttattcaa
     3241 aaaatggaca accttacgcc ggataattta gatgaattat ctttgagctt ttatttatgt
     3301 aaatcattaa ttgccctgca tgaaggaagt atttctgtcg aaggaaattc ttttgaaaca
     3361 atgtttactg taaagcttcc atgcgaaaat acagactcaa ttaattattt gttcatgcga
     3421 gacaataaaa atgaatattt ggctgagcaa atag
//
LOCUS       DAOPUE010000164         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_27889_length_3449_cov_7.411019, whole genome shotgun sequence.
ACCESSION   DAOPUE010000164 DAOPUE010000000
VERSION     DAOPUE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27889_length_3449_cov_7.411019"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PKH42_09755"
     CDS             complement(<1..1039)
                     /locus_tag="PKH42_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HOA20760.1"
                     /translation="METLMNINSAINSVVWGPVLLILVLGTGTYLSFMTGFFSITKIG
                     YVLKNTLFKMFAKDDKGEGEVTAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WLAAIMGMTTKYAEVVLAVNYREKTEDGRFVGGPMYYITNGLGKSWKWLAVLFAFFGT
                     FASFGIGCMVQSNSVALSAQSTFGIPVLATGIVLAILTAVVIVGGIKRIGAFTEKLVP
                     FMAALYILGGLWLLISNAAKIPAAFAMIFSNAFTGTAAVGGFTGTVMMQAIRFGIARG
                     IFTNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFTDTIVICSITALSILVTGVWETG
                     ESGAVLSAMAFD"
     gene            complement(1225..1914)
                     /locus_tag="PKH42_09760"
     CDS             complement(1225..1914)
                     /locus_tag="PKH42_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOA20761.1"
                     /translation="MVLSVENLSKSFGKNMVFHDISFSVNKGEIVCIRGKSGEGKTTL
                     LRCLTSLETPDKGSIRINDMYLCKEENNIIKYAQKNELQAIRQEIGLVFQNFNLFPHM
                     NVMENLMEAPLFLKKDTHENIKNRAEELLKSLELEGKENSYPFQLSGGQQQRVAIARA
                     CMLNPSVLCFDEPTSALDDQTRGQISKIIRYLASQGIAIIIVTHDNAFADEIAQKIIT
                     MKDGRITIEEQ"
     gene            complement(1924..2580)
                     /locus_tag="PKH42_09765"
     CDS             complement(1924..2580)
                     /locus_tag="PKH42_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOA20762.1"
                     /translation="MEAKVLRMLVPMALEGLKVTMGVFAVTLIMSLPLAIVVAKLRMS
                     KNKIVSKITGTYIYIMRGTPLLLQLMFIFFGFYRVPYIGYPFSREQAIVIAFVLNYTA
                     YFAEIFRGGINSIDVGQQEASLVLGLSKTHTFMKIILPQVIKNVMPSIGNEVITLVKD
                     TSLVYILGSSDILKAARSVSNVYAAFSPYIYIGILYMVIIAILTKILDKIENKYDYYR
                     "
     gene            complement(2592..3374)
                     /locus_tag="PKH42_09770"
     CDS             complement(2592..3374)
                     /locus_tag="PKH42_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOA20763.1"
                     /translation="MKKNVSKKVKLAVLLILTAILAVGCAAQSSQPETEELIMGLDDT
                     FAPMGFRDEKGELVGFDVDLANEVAERIGVTIKFQPIDWSMKETELNAGNIDLIWNGY
                     TITAERQEKVAFTKPYLENSQIIVTLADSDINTKVDLAGKNVAVQAESSALDAINSEP
                     EVAESFGELVEFSTNNEAFSDLESGRTDALVVDEVNARYFMKQVGEEKYKVLDEDFGD
                     EEYGIGLRKEDTELLKKINDAMDEMKKDGTYDAIYAKWFSEN"
BASE COUNT         1035 a          737 c          537 g         1140 t
ORIGIN      
        1 tatcaaatgc cattgctgaa agaactgctc ctgattctcc tgtttcccaa acgcctgtaa
       61 ccaatatgga aagagctgta atagagcaaa taactattgt atccgtgaat acttcgaata
      121 ctccccacaa gccttgacga acaggatggt cagtagttgc agcagcatgt gctattggag
      181 cactacccaa gccggcttcg ttagtgaaaa taccacgagc aataccaaaa cggattgcct
      241 gcatcataac agtacctgta aaaccaccga ctgcagccgt acctgtgaat gcattagaaa
      301 aaatcattgc aaaagcagca ggtatttttg cggcatttga tattaataac caaagtccac
      361 caagaatgta aagtgctgcc ataaagggaa ctaacttttc ggtgaaagct ccaattcttt
      421 taataccgcc aactataaca actgctgtta gtatagccaa aacaatacct gttgctaata
      481 ccggtatacc aaatgtggat tgtgctgaaa gagcaacgga gtttgattga accatacagc
      541 caataccgaa agaagcaaaa gtaccaaaaa atgcaaataa aacagcaagc cacttccagc
      601 tcttgcctaa gccgtttgta atgtagtaca taggaccgcc tacaaaacgg ccgtcttcag
      661 ttttttctct gtagtttaca gccaatacaa cttccgcata cttagttgtc atacccatta
      721 ttgcagcaag ccacatccaa aataccgctc cgggtcctcc aagagctatt gcggtagcaa
      781 cacctgcaat attacctgtt ccgactgtag ccgcaagagc agtggcaact gcctggaatg
      841 cagttacttc accttcacct ttgtcatctt ttgcaaacat cttaaacaag gtgttcttaa
      901 gtacgtagcc tattttagta attgagaaaa atccggtcat aaatgacaaa taagttcctg
      961 tacctaatac taaaatcaat aagacaggac cccataccac gctgtttatg gcgctgttga
     1021 tgttcattaa tgtttccaaa gtatccctcc attcaaattg ttttaaaaac ataaacatct
     1081 tcttgttaag tatttacatt ttttaacgtt ttatacaatt taatatcaga ctgatactcg
     1141 ggcaaataat acaaggattg atttatgcat tttgcgctga tttaccattt ataaacggca
     1201 agtgtgatag caaaaaaaaa agagctattg ctcttcaata gtaattcttc catccttcat
     1261 tgtaattatt ttttgtgcta tttcatctgc aaatgcatta tcatgggtaa caataattat
     1321 tgcaataccc tgtgaagcca aatatcttat tatttttgat atttgacctc ttgtttgatc
     1381 atctaaggct gaagtaggtt catcaaagca caatacggaa gggttcagca tgcatgctcg
     1441 tgcgattgca acacgctgct gctggcctcc tgacaactga aatgggtagc tgttttcttt
     1501 tccttccaat tcaagacttt ttaaaagttc ttcagctcta tttttaatat tttcatgagt
     1561 atcctttttt aaaaatagcg gtgcttccat taaattttcc attacattca tatgaggaaa
     1621 taaattaaaa ttttgaaata caagtccaat ttcctggcgg attgcctgca gctcgttttt
     1681 ttgagcatat ttgataatat tgttttcttc tttgcataaa tacatatcat taattctaat
     1741 tgagccttta tccggagttt ccaacgatgt aaggcatctt aagagggttg tttttccttc
     1801 tccggattta cctcgtatgc atacaatttc acctttattt acagaaaagg agatatcatg
     1861 gaacaccata ttttttccga agcttttgct taaattttct accgataaaa ccataataac
     1921 ctcctatctg tagtaatcat acttattttc tattttgtcc aatatttttg taagaattgc
     1981 aattataacc atgtataaga tccctatata aatgtatggg ctgaaggctg cataaacatt
     2041 cgatacagac cttgcagctt ttaaaatatc gcttgagcct aatatataaa ccaatgatgt
     2101 atcctttacc aatgtaatta cttcgttgcc tattgagggc ataacatttt ttataacctg
     2161 agggagaata attttcataa atgtatgtgt cttgcttaag cccaatacaa gagatgcttc
     2221 ctgttgaccg acatctatgg aatttattcc tcctctgaat atttctgcaa aataagcagt
     2281 ataatttaaa acaaaagcaa tgacaatagc ctgttctctg ctgaagggat atccgatata
     2341 tggcacacga taaaatccga agaatataaa cattaactgc aatagtaacg gcgtacccct
     2401 cattatataa atataagttc ccgtaatttt tgaaactatt ttgttttttg acattctaag
     2461 ctttgcaacc actatggcca aaggaaggga cataatcaat gtaaccgcaa aaacccccat
     2521 agttaccttt agcccttcca atgccatggg cacaagcatt ctcaaaactt ttgcttccat
     2581 attacattcc tttaattttc agaaaaccat tttgcataaa tagcatcata tgttccgtct
     2641 tttttcattt cgtccattgc gtcgtttatt tttttcaata actccgtatc ttcttttctc
     2701 aagccgatac catattcttc atcaccaaag tcttcatcta atactttgta tttttcctca
     2761 ccgacttgct tcatgaagta tctcgcattt acttcatcta ctaccaaagc atctgtacgc
     2821 cctgattcca aatcgctgaa tgcttcgttg tttgtggaaa actctactaa ttccccaaag
     2881 gattcagcta cctctggctc ggaatttatt gcatccaatg cactggattc agcttgcacg
     2941 gcgacatttt tacctgccaa gtcaactttt gtattaatat ctgagtcggc taaggtcacg
     3001 attatttggc tgttttccaa gtaaggttta gtaaatgcca ctttttcttg tctctcggca
     3061 gttatggtgt agccgttcca aattaaatca atatttcctg catttaattc ggtttccttc
     3121 attgaccagt cgataggctg gaatttaatt gttacaccga ttctttctgc aacctcattt
     3181 gctaagtcca catcaaatcc taccaattct cctttttcat ctctgaaacc catgggcgca
     3241 aaggtatcat caagtcccat tattagctct tcagtttcag gttgtgatga ttgtgctgca
     3301 caacctacag ccaagattgc ggttaaaata agcaataccg ccaatttaac tttttttgat
     3361 acattttttt tcatttttct cttctcctct tatttaataa tcataataat aatatcacag
     3421 gctccatcac caatttgctt atttgggga
//
LOCUS       DAOPUE010000165         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_28482_length_3393_cov_9.984122, whole genome shotgun sequence.
ACCESSION   DAOPUE010000165 DAOPUE010000000
VERSION     DAOPUE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28482_length_3393_cov_9.984122"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="PKH42_09775"
     CDS             complement(<1..896)
                     /locus_tag="PKH42_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20764.1"
                     /translation="MREILIGLIFALTFTPVNAEETFTNTIEYLQPDDTNDATYPEIP
                     QLPEIDKSTELIKYSPEYNKYINSAYGYEITIPNNLTLNEDIVSVKSRFESDDLVVEV
                     FYDNFYNTINSHATHRNYCNRGILKNPEFKITDEYNHSIGGLSGHVTLFERRKVKTNQ
                     PDRNYYATITFEKNTYESITVFIKSAAPINIDDIMPEFKFTAKTETLKNDAVFHPEPK
                     NFDEKTRDFYDKYFINNEKTAFGIFEPTFPLYAYRLLQIEKMLDYNFPVALLYNNFSL
                     PFKTDYMNKAKELGKVVEYGLYT"
     gene            1064..1486
                     /locus_tag="PKH42_09780"
     CDS             1064..1486
                     /locus_tag="PKH42_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOA20765.1"
                     /translation="MVTADTFKRGFKNGISTLIDLSKVLVPVYIGVELLAASGFLNIL
                     AEVFKPVMSTLGLPGEASLIIILGYFLGTYAGLGAIAAMELNTVQITTIAIMLSISHS
                     LISETAIVKKLGASAAASITVRVFFSFFMGFIYYRIFG"
     gene            1491..1958
                     /locus_tag="PKH42_09785"
     CDS             1491..1958
                     /locus_tag="PKH42_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOA20766.1"
                     /translation="MSGIMDILINLLSFLWNITKVLIPIMIVIEIFKDAKLIDKISDF
                     FYPVTKFFTLSKISGISLIFGMFFGLTLGAGAILQSVKDYNVDKRSVFLVAVFLSPCH
                     AIFEDAFIMKAAGSNILPLLAARFISAIAATYIFSRAVKNNTTDVVNISNHEK"
     gene            complement(2040..3290)
                     /gene="hisS"
                     /locus_tag="PKH42_09790"
     CDS             complement(2040..3290)
                     /gene="hisS"
                     /locus_tag="PKH42_09790"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOA20767.1"
                     /translation="MKINLNPAKGMRDITPREKEIRDYVEGVIVDTYKKSGFELFETP
                     VVENIENLTGSQGGENLMLIFKILKRGDKLDLNKEGLTEDDLSDLGLRYDLTVPLSRF
                     YCNNRSNLLPVFKALQVGNVFRAERAQKGRYRSFKQCDIDIIGDGTQAAELELIATTA
                     KALMALNVKDFVVRFNDRRVLKAVILDCGFKEDEFDGVCIVVDKLDKIGMSGIKNELE
                     DKGYDKTSSEKLFIALEEINNKGTDCLSEYGVPNEVVTDVKKVLEESNTYAAGKYEVV
                     FDFTLIRGMGYYTGSIFEIAYKDLGYSIAGGGRYDEMVGNFIGEKIPAIGFSIGFERL
                     VNQLMEENFKVPDLEKVVLLYEPSDRYIDVLAKAESIRKQGYIVSVYEKSKKLGKQLN
                     QFQGYGYKKFAVYEGDETEIKELN"
BASE COUNT          950 a          623 c          551 g         1269 t
ORIGIN      
        1 gtatataagc cgtattccac aaccttccct aattccttcg ctttattcat ataatctgtt
       61 ttaaagggca gagaaaaatt gttgtagagc aatgctaccg gaaaattata atcaagcatt
      121 ttttctattt gcaaaagcct gtatgcatat aaaggaaatg taggctcaaa aataccgaaa
      181 gctgtttttt cgttgttaat gaagtattta tcgtaaaagt cccttgtttt ttcatcaaaa
      241 ttctttggct cgggatgaaa gaccgcatca tttttcaaag tttcagtctt agccgtaaat
      301 ttaaattccg gcataatatc atcaatattt atgggtgctg ctgattttat aaatacggtt
      361 atggattcat atgtattttt ttcaaatgta attgttgcgt aataattcct gtccggttga
      421 ttggttttta cctttctcct ttcaaagagt gttacatgcc ctgatagtcc gccgatacta
      481 tgattgtatt catctgtaat tttaaattca ggatttttta atatgcctct gttgcagtag
      541 tttcgatgtg tagcatgtga atttatcgta ttgtaaaaat tatcataaaa aacttccact
      601 accaggtcat ccgattcaaa tcggcttttt acgcttacta tatcttcatt aagcgtaaga
      661 ttatttggaa ttgttatttc atacccgtag gcactattta tatacttatt gtactctgga
      721 gaatatttta ttaactctgt acttttatca atttccggaa gctgtggaat ctccggatac
      781 gttgcatcgt ttgtatcgtc aggttgaaga tattctattg tgttggtaaa tgtttcttct
      841 gcattgacag gtgtaaatgt aagtgcaaaa attagtccga ttaatatttc tctcatgtaa
      901 ttctccttgg tcttattcat tcttattaat tttatcaaag atagcaatta tagtcaaact
      961 caattgtgtc acttccctta ttaaccgtaa cgtttagaat cgcagaagtt ttgattgtta
     1021 ataaataatt tgtatgctat aatttttctg ttaggaggta gcaatggtta ctgcagacac
     1081 ttttaaaaga ggttttaaaa acggtatttc tactcttata gatttatcga aagtattggt
     1141 tcccgtgtac ataggagttg aactgcttgc tgcatcaggt tttttaaata tattagctga
     1201 agtatttaag cctgtaatgt caacattggg actgcccggt gaagcttcac tgataataat
     1261 attaggttat tttttgggaa catatgccgg cttgggtgca attgctgcca tggaacttaa
     1321 tacagtacaa ataacaacaa tagcaattat gctttcaata tcccacagtc tgatatcaga
     1381 aacggcaata gtaaaaaaat taggtgcaag tgcagcagcc agtattacag taagggtgtt
     1441 tttttcattt tttatgggat ttatatatta taggatattt gggtgatatt atgagtggaa
     1501 ttatggatat attgataaat ctattgagct ttttatggaa tataactaag gtactgattc
     1561 caattatgat tgtaattgaa atatttaagg atgcaaaatt aattgataaa atatctgact
     1621 ttttttatcc tgtcacaaaa ttttttacat taagcaagat atccggaata tcccttatat
     1681 tcggtatgtt tttcggactg acacttggcg cgggtgccat ccttcaaagt gtgaaggact
     1741 acaatgtaga caaaagaagt gtttttttag tagccgtgtt tttgtccccg tgtcatgcaa
     1801 tatttgaaga tgcatttata atgaaagctg caggctcaaa tattttgccc ttgttagcag
     1861 caagatttat ttctgcaatt gctgcaacat atatattttc acgagctgta aaaaacaata
     1921 cgactgatgt tgtaaatata agcaaccatg aaaaatgaga ttcttcgggc tgcgccctca
     1981 gaatgacgat tgataggtca ttcagtgctg caggtgaaga atctcatagt atctattctt
     2041 taatttaatt cttttatttc ggtttcgtct ccttcgtaaa cggcgaattt tttgtatccg
     2101 tatccttgaa attgatttaa ttgcttgcct aatttcttgg atttctcata tactgatacg
     2161 atatagcctt gttttcttat actttcagct tttgcaagaa catctatgta tctgtccgat
     2221 ggctcatata aaagcactac tttttccaaa tcgggaactt tgaagttttc ttccatcagc
     2281 tggttaacca gtctttcaaa tcctattgaa aagcctatgg caggtatttt ttcacctatg
     2341 aaatttccta ccatttcgtc gtatcttccg ccgccggcta ttgaatagcc taaatcttta
     2401 tatgcaattt caaatattga gccagtgtag tatcccatac ctcttattaa ggtaaagtca
     2461 aacactactt catattttcc ggctgcatat gtattggatt cttcaagtac cttttttaca
     2521 tctgtaacta cttcatttgg aacgccgtac tcgcttaaac aatcggtccc cttattattg
     2581 atttcttcca atgctataaa taacttttca gatgaagttt tgtcatatcc tttgtcttcc
     2641 aattcatttt taattccgct cattccgatt ttgtccagct tgtcaactac gatgcatact
     2701 ccgtcaaatt catcttcttt aaatccgcag tccagtatta cagcctttaa tactcgcctg
     2761 tcattaaaac gaaccacaaa atcctttaca ttaagtgcca tgagagcttt ggctgtcgtt
     2821 gcaatcagtt ctaattccgc tgcctgcgta ccgtctccta ttatatctat atcgcattgt
     2881 ttaaagcttc tgtatcttcc tttttgagcc ctttcagccc tgaacacatt ccccacctgc
     2941 agagctttaa atacagggag taaattagag cggttgttgc aataaaaacg gcttaaagga
     3001 actgtcaaat cgtatctaag tcctaaatcg gacaggtcat cttctgttaa tccttcctta
     3061 tttaaatcaa gtttatctcc tctttttaat attttaaaaa taagcattaa attttctccg
     3121 ccttggctgc ctgttaaatt ttctatattt tcaacaacag gtgtttcaaa cagctcgaaa
     3181 ccgctttttt tatacgtatc gactattacc ccttccacat agtctctaat ttccttttct
     3241 cttggcgtta tatctctcat tccctttgca gggtttaaat taattttcat ccgttctgcc
     3301 tcctacctta tgggacagtg gggacgttcc tgactgtccc actttttgtc cctaattcta
     3361 attttatata ttgttaacta ttttaccaaa tag
//
LOCUS       DAOPUE010000166         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_28631_length_3380_cov_11.573835, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000166 DAOPUE010000000
VERSION     DAOPUE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28631_length_3380_cov_11.573835"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..1313
                     /locus_tag="PKH42_09795"
     CDS             204..1313
                     /locus_tag="PKH42_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA20768.1"
                     /translation="MTYVIYTVQPGDTLYSIARMYGSSIQGIIDTNNMENPDLIYPGS
                     VILIPVEEEYLETPPGSLIYTVQPGDTLYIISLLFKVSTQRIMELNDIPDPSLIFPGM
                     KIILPLEAVNPFQPVVPGIIRYTVLPGDTIYKIAARFGTTTQLILNANPGLDPRRLFP
                     GMVITITIPENAVAIYRGNPNRRMVSLTFDATYGDNQTHELLEILRNNNIKATFFLSG
                     IWLINYPELARAIAAEGHEIGNHSLTHPHMPLLTLQAVTNQIVRTEALIRNITGQDPY
                     LFRPPFGEYTQAMLNQLASLGYVTIMWTVDSLDWKNPGPAAVVSRVVNNAVPGAIILL
                     HQSAPDTLNGLQDMITQLRQGGYDFGTVTQVISPL"
     gene            complement(1388..2962)
                     /locus_tag="PKH42_09800"
     CDS             complement(1388..2962)
                     /locus_tag="PKH42_09800"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOA20769.1"
                     /translation="MKRKIFLFLVILMMAAAIIPENKAEAANSVRVSLPAFKVSLNGV
                     NIENNYNQYPLIVYNDITYFPMTYYDSRFMGLETAWDGSTGLKIIKTGANWSYQKYES
                     STKNSSIYNAQIAPFKITVNGKTIDNSKEQYPLLLFRNITYFPLTWRFAVEEFGWKYT
                     YNQKNGLAVNSTDSTPSSGQVILPIVTHETGQKGAFTMAGDYFYYEGANGIIYQVPVN
                     APASAKKVYELPDMSYQGIANYDYFTSLKTENGKAFLSYHTGGATMGSDHLIWLKDDG
                     TYEVLDSGYSLMKIYDNYTVRVEQRGPFFAENLQIKKNGEKDYVNVGKSNFSYGMYIY
                     KDVYNNSRAATPSKDLYLIGDEIYVLGYYGYFLENTDDQTTGIYRVNINSNEQARLCD
                     NEVTSFKILDDTIFFADQNNFIYKVPLAGGKAERLVDEAVNSYEVLEGKLYYSLKNNN
                     QLYVYGNDESVNPGGILKSLEVQNGYIIAIFDKLSESQYKLMIFNEEGKVLYKSIENV
                     LLVRIDNGKVVFVKDN"
     gene            complement(2991..>3380)
                     /locus_tag="PKH42_09805"
     CDS             complement(2991..>3380)
                     /locus_tag="PKH42_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20770.1"
                     /translation="IAVILFVIISGTIFNVTGVEEPEKVSSENEFLDEEDGIANENNV
                     VNENEVKAQTEENELEIYNKFENSEPETLPATENIQQDNTRQIEINQEGSIKTHEEAM
                     VENDMSEIVIVDLNQLEDQLKESETVE"
BASE COUNT         1025 a          673 c          558 g         1124 t
ORIGIN      
        1 tcgatttttc tgcaatttaa tacatattat attacatatc cgaattattt caacacaatt
       61 tgtacgtcaa ggagacgtat gacaaggtga cgggggtgct ttcatatgat aagtagacgg
      121 ggatgttgaa gtaaattcat aattatcact ttgtttgagc tgtggaatat tatatgaata
      181 aagaatagga ggaccattat aatatgacat atgtaatata tacagtacaa cccggtgata
      241 cgctctatag tattgcccgt atgtatggaa gcagcataca aggaattata gatacaaata
      301 atatggaaaa cccggacctt atataccccg gctctgttat actgatacct gttgaagagg
      361 aatacctgga aaccccgccc ggaagcctta tttacaccgt acagccaggc gatactttat
      421 atataatttc tctattgttc aaggttagca ctcaaagaat tatggaacta aacgacattc
      481 ctgacccttc attgattttt ccgggaatga aaattatact gccgttagaa gccgttaatc
      541 cgtttcagcc tgtagtaccc ggtataatca ggtatactgt gctgcctgga gataccatct
      601 ataaaatcgc tgcaagattt ggtacaacaa ctcaattgat tttaaatgcc aatcccgggt
      661 tggatcctcg caggcttttt cccggaatgg tcataacaat tacaatacct gaaaatgctg
      721 ttgcaatata caggggaaat ccaaacagaa gaatggtttc tttaacattc gatgcaactt
      781 acggcgataa tcaaacccat gaattgctgg aaatattaag aaacaacaat attaaggcaa
      841 catttttcct gtccggaatc tggctgataa actatcccga gttggcaaga gccatagcag
      901 ctgaaggtca cgaaatagga aatcatagcc ttacccaccc tcatatgccc cttttaacat
      961 tgcaggcagt aacaaaccaa attgtaagga cagaagctct tataagaaat atcacaggtc
     1021 aggacccgta tttgttcaga ccgccttttg gcgaatatac ccaggcaatg ctgaaccaat
     1081 tggcttcact gggctatgta acaattatgt ggacggtaga ctccttggac tggaaaaatc
     1141 ccggaccggc tgcagtagtc tcaagagttg ttaacaatgc agtacccgga gcaattattc
     1201 tgcttcacca atcagctccg gatacactta acggactgca ggatatgata acgcaattaa
     1261 gacagggagg atacgatttc ggtacagtca cacaggttat aagcccgcta taaatgacaa
     1321 ggggacgggg ggaatgtcat aaacacatga ctgcccgcgt cctctttaat ttacttaaat
     1381 aattccatca attatctttt acgaaaacaa ccttaccatt atcaattcgc actaataata
     1441 cattttcaat ggatttatat aatacttttc cttcttcatt aaaaatcatc aatttatatt
     1501 gagactcgct aagtttatca aatatggcaa ttatgtagcc attttgaact tctaaacttt
     1561 ttagaatgcc tccgggattt actgattcat cattaccgta tacataaagc tgattgttat
     1621 tcttcaatga ataatatagt tttccctcca atacttcata tgaattaaca gcttcatcta
     1681 caagccgctc agccttgcca ccggcaagcg gaactttata aataaaattg ttttggtcgg
     1741 caaaaaatat tgtatcatca agaattttaa aacttgtcac ttcattatca cacaatctgg
     1801 cttgttcatt gctattaatg ttaactctgt aaatacctgt tgtttgatca tcagtgtttt
     1861 ctaaaaaata tccgtaatac cctaaaacat atatttcatc accaattaaa tacaaatctt
     1921 tgcttggagt tgctgcgcga gaattattat acacatcttt ataaatgtac ataccataag
     1981 aaaaattaga tttacctaca tttacataat ccttctcgcc attcttttta atctgcagat
     2041 tttctgcaaa aaaaggacct ctttgttcaa ctcgcacagt gtaattatca tatattttca
     2101 tgagcgaata gccgctatca agaacttcat aggtgccatc atcttttagc catataagat
     2161 gatctgaacc catggttgct ccgcctgtat ggtaactgag aaacgccttg ccattttcgg
     2221 ttttaagaga tgtaaaataa tcataatttg caatgccttg atatgacata tcaggcagtt
     2281 cgtatacctt tttggcactt gcaggggcat ttaccggaac ttgataaata attccattag
     2341 ctccttcata gtaaaagtaa tctcctgcca tggtaaaggc tcctttttga ccggtttcat
     2401 gagttacaat cggcagtatc acttgacccg atgatggagt gctgtctgtt gagtttactg
     2461 ctaaaccgtt tttctgattg taagtatatt tccagccaaa ttcttctaca gcaaacctcc
     2521 atgtcaacgg gaaatatgtt atatttctga ataaaagcaa gggatactgt tctttgctgt
     2581 tgtcaattgt ttttccatta accgtaattt tgaatggagc tatttgtgca ttgtagatac
     2641 tgctgttttt ggttgatgat tcatactttt ggtaactcca atttgcacct gttttaatta
     2701 tttttagccc agtactgccg tcccacgcag tttccagtcc cataaatctg ctgtcataat
     2761 aagtcatagg aaaatatgta atatcattat aaacaataag aggatattga ttataattgt
     2821 tttctatgtt tactccattt aaactaactt taaatgccgg tagtgaaact cttacactgt
     2881 ttgctgcttc cgccttattt tcaggtatta ttgctgcagc catcatcaat ataactaaaa
     2941 acaaaaatat ctttcttttc attattgttc ctccttaagc gcataagtta ctattcaaca
     3001 gtttcgcttt ccttaagctg atcttccagc tgatttaaat caacaatcac aatttccgac
     3061 atatcatttt caaccatagc ttcttcatgt gtttttattg aaccttcttg attgatttct
     3121 atttgccgtg tgttatcttg ttgtatattt tctgtcgccg gcagggtttc cggttctgag
     3181 ttttcaaatt tattgtatat ttcaagttca ttttcttcag tttgagcttt tacttcattt
     3241 tcatttacaa cattgttttc atttgcaata ccatcttctt catccaaaaa ttcattttca
     3301 gaagatactt tttccggctc ttctacaccg gtgacattga atattgtgcc ggatataata
     3361 acaaataata ttactgcaat
//
LOCUS       DAOPUE010000167         3379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_28643_length_3379_cov_8.102587, whole genome shotgun sequence.
ACCESSION   DAOPUE010000167 DAOPUE010000000
VERSION     DAOPUE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28643_length_3379_cov_8.102587"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..2331)
                     /locus_tag="PKH42_09810"
     CDS             complement(160..2331)
                     /locus_tag="PKH42_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20771.1"
                     /translation="MKIKIKIKTLIFAVFILTLTFIWAIPTATLMIAGYLETKDSEKA
                     SLFYEKYASYPTTPSIESKYLHANSLIRSFNKFTIFLTGWGGGENTSPEDIEKAKQIL
                     METLAEKPVKNREKDYYIKSYKMLLDILIASGDAETLQEWIAFGQKGTDERLVYTADV
                     YDGFLHHVNGEGEAAKSIIEKYDETELKDTIFYVLKAEIALFDGKYEESEKIYDNIYK
                     NNWRELGKSFGSGGYYRNDWFDRFMEDFKGDNVITGTVTCEGKPMPFVEIYVQEAKEG
                     FSFYASGENYVAITDVNGEFKTLGLKDGLYSVGIGVDGSVLANKAFQGSTMQYVELNG
                     GDGEFHLSFRNTLKIYSPEPGEKLSGEEFTVSWEEIEDAAYYKVEPVVFYEPFGERGI
                     STRIYAKDKNGEYKFTENQAVFNINMIRNNPYITVSDSDNGYLGAQAVLGIFIPGVEY
                     PIVVNAYDENNNLITGSLPLRSYYEHIPSITAEGSLNEGENFIYRKNYPAAIEYYENI
                     LKENPDDIDALRYLTRIYGIGWKEGEQNFERAFTLGQRYTEISGNRELLTNTLGGMNI
                     SEIKENRENVYLTLKEAMKEPDFDNYYFLSRYYIAVENYEGAREALQMAENVTDNLIF
                     LNMYFGDYKEAVESIKSERFYRSRLSSNKVKDAIEALDESPPRGDELKVFNDFLLKLI
                     NGIPYGETRNIYNKTVKQISNSNLKTIIDEIYLDRNWDLEY"
     gene            complement(2328..2639)
                     /locus_tag="PKH42_09815"
     CDS             complement(2328..2639)
                     /locus_tag="PKH42_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20772.1"
                     /translation="MDKKDKLGIEIKSIMEEETLDLTLSQKAFDNIIQHRKKSLREKI
                     QEFINKEIEIPVAPAIIGFAALLAVTLIPMDIFKSQNERIIDMGGSYVIIREGFEVSK
                     K"
     gene            complement(2639..3166)
                     /locus_tag="PKH42_09820"
     CDS             complement(2639..3166)
                     /locus_tag="PKH42_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20773.1"
                     /translation="MVAEELLILNLKRGREEAYRQLIEKYGNRLYRTCYLILNDREEA
                     EDVVQETFIKVFKKIDTFKGQSGLYTWIYAIALNLSRDRIRMKQDMLELKDEWIGSYD
                     VESLVEINIDRELLKKEIFEMNSLYREVLVLFYFEELSIKEISNLLNEKEGTIKSKLS
                     RGRNILKESLLKGGR"
BASE COUNT          968 a          698 c          438 g         1275 t
ORIGIN      
        1 gggaacaatt cccaaaaatc atcctcgatt ataaatttct tcatttgata cctcccgcac
       61 aatatccttt cttttctcag atacctatta tatcacacaa tttatatcat tacgctataa
      121 ttctacttta cataaattta attatgtaaa gcaaaatgat tagtactcta agtcccaatt
      181 tctatcaaga taaatttcat caataatagt ttttaaattg ctgtttgaaa tttgctttac
      241 ggttttatta taaatattcc gggtttcacc atatggaatg ccgtttatca gttttagcaa
      301 gaagtcatta aataccttta attcatctcc ccttggagga ctttcatcaa gagcctcaat
      361 ggcatctttt actttatttg atgacaacct tgacctgtag aatctttccg attttatact
      421 ttcaacagcc tccttatagt ctccaaaata catgtttaag aaaatcaaat tatccgttac
      481 gttttctgcc atttgcaaag cttctcttgc accttcataa ttttcaacag caatataata
      541 tctgcttaaa aaataatagt tatcaaaatc cggttccttc atagcttctt ttagagtcaa
      601 ataaacattc tctctgtttt cctttatttc actgatgttc atcccaccaa gggtattggt
      661 cagcaattct ctgtttcctg aaatttcagt atatctctgc cccaaagtaa aagctctttc
      721 aaaattttgc tctccctcct tccagccaat tccgtatatc cttgtaaggt atcttaacgc
      781 atctatatca tcaggatttt cttttagaat attttcgtag tattcaatag ctgcaggata
      841 gtttttgcga tatataaaat tttctccttc attcaaactt ccttctgccg taatacttgg
      901 aatatgctcg taataagacc ttaaaggaag gctgccggtt atgagattgt tattctcatc
      961 atatgcattt acaacaatgg gatattcaac tcccggaatg aatatgccaa gaaccgcctg
     1021 agctccaagg tatccgttat ccgaatcact tacagtaata tatgggttgt ttctaatcat
     1081 atttatgtta aatacagctt gattttcagt aaatttgtat tctccattct tgtcttttgc
     1141 atatatacgt gtgcttattc ctctctcccc gaacggctca taaaaaacca ccggttcaac
     1201 cttgtagtag gctgcgtctt caatctcttc ccatgatact gtaaattctt ctcctgatag
     1261 cttttctccc ggctcaggtg aatatatttt tagggtattt ctgaatgaaa ggtgaaattc
     1321 gccatctcct ccattcagct caacatactg catcgttgaa ccttgaaatg ccttgtttgc
     1381 caggacagag ccgtcaactc cgattcccac actgtataat ccgtctttta aaccaagcgt
     1441 tttaaattct ccgtttacat cggttatagc aacatagttt tctccgctgg cgtaaaaact
     1501 aaatccttct ttggcttcct gcacatatat ttcaacaaag ggcatgggct ttccttcaca
     1561 ggttacagtc cctgttatga cattatctcc tttgaagtct tccataaatc tgtcaaacca
     1621 atcatttctg taatatcctc cggaaccgaa gctttttcct aattctctcc aattattttt
     1681 atatatatta tcatatattt tttccgattc ttcatatttt ccatcaaata aagcaatttc
     1741 tgcttttaat acataaaaga tagtgtcttt taattctgtc tcatcatatt tttcaataat
     1801 tgactttgcc gcttcacctt ccccatttac gtgatgaaga aatccatcat atacatcagc
     1861 agtatatact aatctttcat ctgtaccttt ttgtccaaag gctatccatt cttgaagggt
     1921 ttctgcatct cctgatgcaa tcaaaatatc aagaagcatt ttatatgatt ttatataata
     1981 atctttttct ctgtttttaa cgggtttttc cgctaatgtt tccataagaa tttgtttagc
     2041 tttttctatg tcttcagggc ttgtattttc acctccgccc cagcctgtca gaaaaatcgt
     2101 aaacttgtta aaactcctta tcaggctgtt tgcatgaaga tacttgcttt ctatacttgg
     2161 cgtggtcgga taggatgcat atttttcgta aaaaagagaa gctttctcgg aatctttagt
     2221 ctcaagatat cctgcaatca ttaaggttgc tgtcggtatt gcccatataa aagtcaacgt
     2281 aagtataaat actgcaaaaa ttaatgtttt aattttaatt tttatcttca tttcttgctc
     2341 acctcaaaac cttccctaat tattacataa gacccgccca tgtcgataat tctttcattt
     2401 tgtgatttga atatatccat cggaatcaat gtaacagcta acaaggctgc aaaccctatt
     2461 attgcgggtg ctacgggtat ttcaatctct ttgtttataa actcctgaat tttttctctt
     2521 aaagacttct ttctgtgttg aataatatta tcaaaggctt tttgagacag agtaaggtca
     2581 agcgtttctt cttccataat actttttatt tctattccta atttatcttt cttatccatt
     2641 acctgcctcc tttcaaaagg ctttccttca atatatttct ccctcttgat aacttgcttt
     2701 taattgttcc ttccttttca ttcaataaat ttgaaatttc ttttatagaa agctcttcaa
     2761 agtagaatag cacaagtacc tctctataaa gtgaattcat ttcaaatatt tcttttttca
     2821 atagctctct gtcgatattt atttctacca gagattcaac atcataactt ccaatccact
     2881 catctttcaa ctcgagcata tcctgtttca tccttattct gtctcttgag aggttgagag
     2941 caattgcata aatccatgta taaagccctg actgtccctt aaatgtatca attttcttaa
     3001 atacctttat aaatgtttct tgaacaacat cttctgcttc ctctctgtcg tttagtatca
     3061 ggtagcaggt tctatacaat ctattgccat atttttctat taattgtctg taagcctctt
     3121 cacgacctct ttttaagttc aatatcaata attcttctgc taccataaca ctctcccaac
     3181 atttttgacg atgaaatgaa caaaaggttg caaacattta tttgaaataa ataatgtatt
     3241 ctgtttacaa agatttctta ttgaatatat tatactaatg attgaaaaat aatgaaagga
     3301 tgttataaat gcttaatggg aggacatata taaatagaaa agtcaataaa cttgaatcaa
     3361 gtggaaatac aggtttttt
//
LOCUS       DAOPUE010000168         3375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_28683_length_3375_cov_10.593675, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000168 DAOPUE010000000
VERSION     DAOPUE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28683_length_3375_cov_10.593675"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PKH42_09825"
     CDS             complement(<1..521)
                     /locus_tag="PKH42_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOA20774.1"
                     /translation="MNLKVVLKVLGTVLFWESVLMVPPLIISIIDNSYDRDAFIVTIV
                     LVGFIGIMLKSIKVTVNIRKREAYASVALSWLLMSLFGALPYYVSGTAPNYIDALFET
                     ASGFTTTGATIFTNVEILPRSILFWRSFTLWIGGMGVLVFTLALAPSLGARSIFLMRA
                     EFPGPSSGKLVPKL"
     gene            complement(584..1135)
                     /locus_tag="PKH42_09830"
     CDS             complement(584..1135)
                     /locus_tag="PKH42_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOA20775.1"
                     /translation="MAKIINTPLTPELINDLMAGDRVFLNGIIYTGRDAAHKRLAELI
                     EKGEELPMDIKNQTIYYVGPCPAKPGQVIGSAGPTTSGRMDSYAPLLMEHGLKGMIGK
                     GIRNQQVVDSIIKYNAVYFAAIGGAGALLSEAIKEAEVIAFPDLGAEAIYKLRVENFP
                     VIVIIDNKGNDLYKLGKEKYKIV"
     gene            complement(1145..1990)
                     /locus_tag="PKH42_09835"
     CDS             complement(1145..1990)
                     /locus_tag="PKH42_09835"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOA20776.1"
                     /translation="MREINVEVITKTVEKLCIESNYYLPEDVKNALEDAMEKEESPLG
                     KEILSDILKNQKIARTKAVPICQDTGLAVIFLELGQEVRLVGGDLNEAINEGVRRGYL
                     NGYLRKSAVDDPFIERKNTGDNTPAIIHTKIVPGDKIKLIFAPKGGGSENMSALAMLK
                     PSDGIKGIKEFVLNTVEKAGANPCPPIIVGIGIGGTIEKTTLIAKQSLLRNVGEHNTN
                     PKVAKLEEELLEEINKLGIGPQGFGGRITALAVNIETFPAHIASMPVAINIQCHAARH
                     REAII"
     gene            2296..3369
                     /gene="ytvI"
                     /locus_tag="PKH42_09840"
     CDS             2296..3369
                     /gene="ytvI"
                     /locus_tag="PKH42_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOA20777.1"
                     /translation="MTESEKKKFILDIAFIVTVYAVLYFIFVYVIHWVMPFIVGFLIA
                     LALRPVSRFVNRFVKSTGKGVALFVITMFYVVLAIVIWFLTSFLITQISELIHIMPRL
                     YFNRIEPVLLEFNDWVVQNAETLSPNVASTISQIITNGINYLADFIKNISISFVQLAT
                     RLISNFPLYLISVIFTIVLSVFISLEYDNITAFFRRQMPEKFKDTFEEARKFITGTLW
                     KMIKSYILIMIITFIETFTGLSILKVNYALPIAAIIAVLDVMPVLGTGGVLIPWAIFE
                     FILKNYFLGFGLLALYIAVTVIRHIIEPRIVGHQIGLHPIITITAMYAGLRLFSVAGV
                     ILGPIVAITVKYLNDEGKIKLFK"
BASE COUNT          985 a          654 c          538 g         1198 t
ORIGIN      
        1 agctttggaa ctaattttcc ggaagagggt cccggaaatt cagcccgcat caaaaatatt
       61 gagcgtgccc ccagtgaagg tgctaacgcc aaggtgaaca ccaatactcc cattcctcca
      121 atccaaaggg taaaactcct ccaaaacaat atgcttcgag gaagaatttc tacatttgta
      181 aatatagttg ctcctgtagt cgtaaacccg gaagcagttt caaacaaagc atctatataa
      241 ttgggtgcag taccgctgac gtagtatggc agagctccaa ataaagacat caaaagccag
      301 ctcagcgcta ctgaagcata agcttcacgt tttctgatat tcacggtaac tttaatactt
      361 ttcagcataa ttcctataaa gccaactaaa acaattgtaa caataaatgc atctctgtca
      421 taactgttat ctattattga aataataaga ggcggaacca ttaataccga ttcccaaaac
      481 aaaacagttc caagtacttt aagaacaact tttaaattca tttttcctcc ggaaagtttt
      541 tttgttctgt tccggtctct ttgcctgaac aactgtatct ttattaaaca attttgtact
      601 tttctttccc tagtttatat aagtcatttc ctttattgtc aattatcacg ataacaggaa
      661 aattttctac tctcaattta tatattgctt ctgctcccaa atcggggaat gcaattactt
      721 ctgcttcttt tattgcttct gaaagcagtg ctccggcacc tccgatagcc gcaaaatata
      781 ctgcattgta cttgattatc gaatcaacca cttgttggtt cctgataccc ttgcctatca
      841 tacccttaag accgtgctcc attaataaag gtgcatatga atccattctt ccgctggttg
      901 taggtcctgc tgagccaatt acttgtcccg gtttagcagg acatggtccc acataataaa
      961 tggtctgatt cttaatatcc atgggaagct cttctccctt ttcaattaat tcagctaatc
     1021 ttttatgggc tgcatctcgg cctgtataaa ttattccgtt taaaaaaact ctatctcctg
     1081 ccataaggtc atttattaat tccggcgtta agggtgtatt tattattttc gccataacaa
     1141 cctcctaaat tattgcttcc cggtgccttg cagcgtgaca ttgtatattt attgcaacag
     1201 gcatgcttgc tatatgagca gggaaggttt ctatatttac ggcaagtgct gttattcttc
     1261 ctccaaagcc ttgcggtcct atacccagct tattaatttc ttccagcaat tcttcttcta
     1321 atttagctac ctttggattt gtattatgct ctcctacatt tctaagaaga gactgttttg
     1381 caatcaaggt tgttttttct atcgttccgc caatacctat tcccactatt atgggagggc
     1441 atggattagc gcctgctttt tctaccgtgt tcaatacaaa ttctttgatg ccttttatac
     1501 catcagaagg ttttaacatt gcaagagcgc tcatattttc gctcccgccg ccctttgggg
     1561 caaatattaa ttttatctta tctccgggta ctatctttgt gtgaataatt gctggagtat
     1621 tatcgcctgt attttttctt tcgataaagg ggtcatcgac agcagacttt cgaagatatc
     1681 cgtttaaata acctcttctt actccttcgt taattgcttc atttaaatct ccgcccacca
     1741 atcttacttc ctgtcccaac tcaagaaaaa taactgcaag tcctgtgtcc tgacatatgg
     1801 gaactgcttt tgtccttgct attttttgat ttttcagtat gtcagacagt atttctttac
     1861 ccaaaggaga ttcttctttt tccattgcat cttccaaagc attttttaca tcctcaggaa
     1921 gatagtaatt agattctata catagttttt ctactgtctt ggttatgact tcaacgttaa
     1981 tctctctcat gcattactcc ttctataata ttcacgtccc gtaattaacc tcttcattta
     2041 tttatatcaa tatttttaaa acttgtctat acttataata atttttataa catcgaccat
     2101 atggaactat tttctaacat ttgtaaattc atgtaataat tttcttatct attgcaaata
     2161 ataaataata atattataat tgaaaataaa gtacggggac acgaggtacc cgttcatgag
     2221 ggacacgcct tcatggtata gtataggaca cgcctttagg aatgttccca attaaaaaaa
     2281 atcgaggtct tgttgatgac tgaaagcgaa aagaaaaaat ttatattaga tattgctttt
     2341 attgttacag tatatgcggt tctttatttt atctttgttt atgtaattca ttgggttatg
     2401 ccttttatag tgggcttttt aatagctttg gctctcaggc ctgtttcaag atttgtaaac
     2461 aggtttgtga agagcacggg aaaaggcgtt gctctatttg taataacaat gttttacgtt
     2521 gttttagcaa tagtcatatg gttccttaca tcctttttaa tcacccaaat ttcagaactt
     2581 atccatataa tgcccagatt atactttaac agaattgagc cggtgcttct tgagtttaac
     2641 gattgggttg ttcaaaatgc agaaacactt tcaccaaatg ttgcaagtac catttcacaa
     2701 atcataacaa acggcatcaa ttatcttgca gattttataa aaaatatatc tatttctttt
     2761 gtacaacttg ctacaagatt aatatcaaat ttcccgctgt atctaatatc tgtaatattt
     2821 actattgtac tttcggtatt tatcagcttg gaatatgata atattacagc ttttttcaga
     2881 agacagatgc ctgaaaaatt taaagataca tttgaagaag cacgaaaatt cataaccggt
     2941 acattgtgga aaatgataaa atcctacata ctaataatga taattacatt tattgaaaca
     3001 tttaccggct tgtctatatt aaaggttaat tatgccctgc caattgctgc aattatagcg
     3061 gttcttgatg ttatgccggt tttaggaaca ggtggtgtac tcataccttg ggcaattttt
     3121 gaatttattt taaaaaatta ttttcttggg tttggattat tagctttata cattgcagtt
     3181 accgttataa gacatattat agagccaaga attgtaggtc accagatagg acttcatcct
     3241 ataataacaa taaccgctat gtatgcaggt ttaaggctat tttcagtggc aggagtaata
     3301 ttaggtccta ttgttgccat aactgttaaa tatttaaatg atgaagggaa aataaaattg
     3361 tttaaatgat gagat
//
LOCUS       DAOPUE010000169         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_28906_length_3357_cov_10.839491, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000169 DAOPUE010000000
VERSION     DAOPUE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28906_length_3357_cov_10.839491"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1424
                     /locus_tag="PKH42_09845"
     CDS             186..1424
                     /locus_tag="PKH42_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOA20778.1"
                     /translation="MGRRNESPEKKALREMTKEYLKQHPLKDGNDVKDMMREMLSVVL
                     DESLEGELDEELGYTRYDYKNKDTDNSRNGFSKKTMHTSYGDLDINIPRDRNGEFEPQ
                     IIKKHQNTITQDMEEKILSMYAKGMTTSDIESHFQDLYGIDVSDSTISRITDKILPIV
                     KEWQERPLESLYAVVFMDAIHFHVRSEGRIIKKAVYIAIGINMDGIKEVLGMYVGENE
                     SAKFWLSIMNGLKNRGVEDILIACVDGLSGFPQAIEAVFPRTELQQCIIHQIRNTTKF
                     VSYKEIKPLMTDLKIVYAAPTEAIALTELDSFEEKWRLKYPKVAKSWRDNWANLSTYF
                     KYPEEVRRLIYTTNTIEGFNRQLRKVTKSKSVFPNDDSLLKMLYLAMMDITKKWTGRR
                     QDWGKIHSQLVIFFEERIKE"
     gene            complement(1703..2878)
                     /locus_tag="PKH42_09850"
     CDS             complement(1703..2878)
                     /locus_tag="PKH42_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOA20779.1"
                     /translation="MLKITDTVSWVGKIDWELNKFHGDEYSTHKGSSYNSYLIRDEKT
                     VLIDTVWEPFSKEFVANLKKEIDLKEIDYIVANHAEIDHSGALAELMREIPDTPVYCT
                     KNGAKIIKGHYHQDWNFVEVKTGDTLDIGKNKLVFVEARMLHWPDSMMTYMAGENILF
                     SNDAFGQHYASELMCNDKVDNYELYNEAIKYYANILTPFSKFVVSKIEEVVALNIPIN
                     MICPSHGIIWRENPLQIITKYMEWAKSYQENQITLVYDTMWKGTRRMAEAIAAGIRSK
                     DKDVDIKLFNCAQKDKNDIITEIFKSKMVLLGSSTINNGILTAAAGILEMIKGLKFTN
                     KKAAAFGSYGWSGEAVKIITDQLKEAKFDVVNDGIKELWNPDEEALERCREFGRNLV"
     gene            3236..>3357
                     /locus_tag="PKH42_09855"
     CDS             3236..>3357
                     /locus_tag="PKH42_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOA20780.1"
                     /translation="MKKKVMVAMSGGVDSSVAAVLLGDQGYDICGATLKLFSDE"
BASE COUNT         1140 a          577 c          608 g         1032 t
ORIGIN      
        1 aggatggagg tcttataagg cttgaaggtc aaagaaaaat tattattaca gatattgaag
       61 gcttaagaga tatacaatag acggagtcac gagactccgt ctgcatttat gggtctgatt
      121 caaatattgt gtaaacctct aaactgatgt aaaataaaaa tactcagttt ggaggttttt
      181 caattatggg aagaagaaac gaatcaccag agaaaaaggc actaagagaa atgacaaagg
      241 aatatttaaa gcagcatcca ttaaaagatg gaaatgatgt aaaagacatg atgagagaga
      301 tgctctcagt tgtgctagat gaaagcttag aaggcgaatt agacgaagaa ttaggttaca
      361 ccaggtatga ctacaagaac aaagatactg ataacagcag aaacggcttc agcaaaaaaa
      421 caatgcacac aagttatgga gaccttgata tcaatattcc aagagatcga aatggtgaat
      481 ttgaaccaca aattattaag aaacaccaaa atacaattac gcaggatatg gaagaaaaaa
      541 tactatccat gtatgcaaaa ggaatgacta catctgatat agaaagccat tttcaggatt
      601 tatatggaat agatgtttcc gacagtacaa taagcagaat aacagataaa atactgccaa
      661 tagtgaagga atggcaagaa agaccattgg aaagccttta tgcagtggta tttatggatg
      721 caatacattt ccatgttaga agtgaaggaa gaataattaa gaaggctgtt tatatagcaa
      781 taggaataaa catggatggt attaaggaag tcctaggcat gtacgtaggc gaaaatgaaa
      841 gtgcaaaatt ctggctatcc ataatgaatg gattaaaaaa tcgaggtgta gaagatatac
      901 tgatagcatg tgtagatggc ttaagcggct ttcctcaggc aatagaagca gtatttccac
      961 gaacagagct acagcaatgt ataatacacc agattagaaa cactacaaag tttgtatcat
     1021 ataaagaaat caagccatta atgacagatt taaaaatagt atatgcagca ccaacggaag
     1081 caatagcttt aacggaactt gattcctttg aagaaaagtg gagattaaaa tacccaaaag
     1141 tagcaaaatc ttggagagat aattgggcta atttatcaac atatttcaag tatccggaag
     1201 aagtcagaag attgatttac accacaaata caattgaagg atttaatcga caacttagaa
     1261 aagttacaaa aagcaagtca gtatttccta atgatgacag ccttctgaaa atgctgtatt
     1321 tagctatgat ggacattacg aaaaaatgga caggccgccg tcaagactgg ggtaaaattc
     1381 attcccagct agtaatattc tttgaagagc gaataaaaga atagtctcta aaaaagggaa
     1441 ggtatttttg tagtagtagt gtttgtggag cttgtggtaa actcgataga gttttccaca
     1501 aatccataaa cacaattgaa tttatctggc atttttgtta tgtaaaatgt cttaattgac
     1561 attacataaa aaacctatat aataaaacaa aaggcagagc ttgttattaa gcctaccttt
     1621 agaaaaaatc atttcaaaaa ttacatcggt tttttgtatt tacacaaaac ttgaaacggt
     1681 ctcgcattta tacccgttct atttatacca ggtttcttcc aaattccctg catctctcca
     1741 aggcttcctc atcaggattc caaagctctt taataccgtc attaaccaca tcaaactttg
     1801 cttctttcag ctgatctgta ataattttta cagcttctcc gctccaaccg tagcttccaa
     1861 atgctgcagc ttttttattc gtaaacttca aaccttttat catttccaaa attcctgctg
     1921 ctgcagtaag aataccatta ttgattgttg atgagcccag aagaaccatt tttgacttga
     1981 aaatttctgt aattatatcg ttcttatcct tttgtgcaca gttgaacaat ttaatatcaa
     2041 catccttgtc ttttgaccgt attcctgctg caattgcttc agccattctt cttgtgcctt
     2101 tccacatggt gtcataaaca agagtgattt ggttttcctg atagcttttt gcccattcca
     2161 tatactttgt aattatctga agaggatttt ctctccagat tattccatgg ctggggcaaa
     2221 tcatatttat gggtatattt aaagcaacaa cctcttcaat tttgcttaca acaaatttac
     2281 tgaagggtgt cagtatattg gcataatact ttattgcctc attgtacagc tcgtaattat
     2341 ccaccttgtc attgcacatc aactctgatg catagtgctg accaaaggca tcattgctga
     2401 ataaaatatt ttctccggcc atgtatgtca tcattgaatc cggccagtga agcattcttg
     2461 cctcaacaaa gacaagctta tttttgccta tatccaatgt atcccctgtt ttaacctcta
     2521 caaagttcca atcctgatgg tagtggcctt taatgatttt agctccattt ttggtgcagt
     2581 atacaggggt atcgggaatt tccctcatta attcggctaa ggcacctgaa tgatcaattt
     2641 ctgcatggtt tgctactatg tagtcaattt cctttaagtc aatttccttt tttaaattag
     2701 cgacaaattc cttcgaaaaa ggctcccaca cagtatcaat cagcactgtt ttttcatccc
     2761 ttattaaata agagttataa gatgaacctt tatgagttga gtattcgtcc ccgtgaaatt
     2821 tgtttaattc ccagtcgatt ttacccaccc agcttactgt atcagtaatt tttaacatat
     2881 tatcctcctg aagtttaact tgtattagtt gtattactta atataccatt tatctttgat
     2941 ttaaatcaat aaaactatac tttttcagct atgttttcaa aatattatta ctgttcataa
     3001 tcagcattaa gtgacttggg atgaattgca aagcttcgcc gtatgacagc cttggatgtg
     3061 aaaggtaaca aacatatgcc ttctaaatta ttgacatggg tatactgtaa ttatataatg
     3121 aattaactaa tagattcaag gaaggggaca aaccttcgaa acgaggcagt cacttggata
     3181 gaccttgaag gaatgtcccg gtatttatat accggtttaa ttaaaaagga gatatatgaa
     3241 gaagaaagtt atggttgcca tgagcggggg agttgattca tcagttgctg ctgtccttct
     3301 cggggatcag ggttacgata tatgcggagc aactttaaaa ttgttctccg atgaaga
//
LOCUS       DAOPUE010000170         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_29542_length_3305_cov_9.474769, whole genome shotgun sequence.
ACCESSION   DAOPUE010000170 DAOPUE010000000
VERSION     DAOPUE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29542_length_3305_cov_9.474769"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..454
                     /locus_tag="PKH42_09860"
     CDS             230..454
                     /locus_tag="PKH42_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOA20781.1"
                     /translation="MPIIDFTKTVYELYKDNEEIAKILEEIGFKDITKPNMINTMGRF
                     MTIPKGARAKGFNMDEIKNEFIKRGYEIKE"
     gene            467..1663
                     /locus_tag="PKH42_09865"
     CDS             467..1663
                     /locus_tag="PKH42_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOA20782.1"
                     /translation="MSENINNREYRKNVIKQVIRELHEGKTVEEVKHKFEEAFAGVSA
                     SEISEAEQALINEGLPVSEVQRLCDVHSAVFKGSIEEIHRETDPMKIPGHPANVLYLE
                     NRRLERIIEEKIKPYLEKGKESLEAIQNGLEELSVIDIHYSKKENLLFPYMEKYGITA
                     PPKVMWGVDDEIRDDLKTLKSDLSYGVFNDELKKGVEELINRINEMIFKEESIMLPML
                     LDIMTQDEWKKVADDSSEIGNIIDFVPVWNPDKEAKKEHKEDIKEPGAIILPTGVFNT
                     EELTHMLNTLPIDITFVGSDDKVKYFSQSSERIFARTKTIIGRNVSNCHPPASVHIVE
                     GIVEDFKSGKKDHEDFWIKMGDKYVYIRYFAVRNEEGKYLGVVEVTQNIKPIQEITGE
                     KRLVED"
     assembly_gap    1665..1764
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1865..>3305
                     /gene="glpK"
                     /locus_tag="PKH42_09870"
     CDS             1865..>3305
                     /gene="glpK"
                     /locus_tag="PKH42_09870"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOA20783.1"
                     /translation="MEKKYVLALDQGTTSSRAILFDRNGRIVNISQKEFTQLYPNPGW
                     VEHNPLEIWETQLNAAKEAIKYIDVSEIACIGITNQRETTILWDKNTGKPVYNAIVWQ
                     SRQTSDICDDLKKSGLEPYIKENTGLVIDAYFSGTKIKWILDNVEGARERAEKGEILF
                     GTVDTWLLWNLTGGKAHITDYSNASRTMIYNIKNLRWDEKILGILDIPSEILPEVRQS
                     SEIYANTNAEIFGAEIPISGIAGDQQAALFGQLCFQEGMVKNTYGTGCFMLMNTGEKI
                     VQSHKGLITTIAWGLQGKVEYALEGSVFVAGAAIQWLRDELKIIHEAADSEYFANKVK
                     DTKGVYVVPAFTGLGAPYWDMYARGAIVGLTRGAGRNHIIRATLESIAYQTKDIIEAM
                     IEDSGINLTVLKVDGGASANNFLMQFQSDILNVNIERPEITETTALGAAYLAGLATGF
                     WKSKGEVIQNWSMNKKFKPSINDDERNRLY"
BASE COUNT         1202 a          440 c          697 g          866 t
ORIGIN      
        1 tgctatccac catctttttt actaaaaaag ttgcacttac tttgtcaatt caccaatagt
       61 tttatatttg ttataattaa catatcatat tcttcattta agaatcaata agtctaatat
      121 taggatagag caaattaata tctttattca aggataataa catataatga aaataaagtt
      181 taaacgagta taaaaaaggt ataagaataa tatgtgatag gaggtaatta tgccaataat
      241 tgattttact aaaacagtct atgagttata taaggataat gaagaaattg ctaaaatttt
      301 agaagaaata ggattcaaag atattacaaa accaaatatg attaacacaa tgggtagatt
      361 tatgacaatt ccaaagggag ccagagcaaa aggtttcaac atggatgaaa taaaaaatga
      421 atttattaaa agaggttatg aaataaaaga gtaaggagag gagcaaatga gcgaaaatat
      481 taataacaga gaatatcgta aaaatgttat caagcaggta ataagggagt tgcatgaagg
      541 taagactgtc gaagaggtaa aacataagtt tgaagaggct tttgcaggag tttcggcttc
      601 agaaatatcc gaagcagaac aggcattaat caatgagggg ttgcctgttt ccgaagtgca
      661 gaggctgtgt gatgttcatt cggctgtttt taagggctcc atagaagaaa tccatcggga
      721 aacagaccct atgaaaattc ccggtcatcc tgccaatgta ttgtatcttg aaaacaggag
      781 attagaaaga attatcgaag aaaaaatcaa accttatttg gaaaaaggaa aagaatctct
      841 tgaagctatt caaaatggat tagaagaatt gtcagttata gacatccatt attccaaaaa
      901 agaaaatctt ttgtttcctt acatggaaaa gtacggaata accgctccgc ctaaggttat
      961 gtggggtgtg gacgatgaaa taagggatga tttgaaaacg cttaaatcag atttatccta
     1021 tggtgtattt aacgatgagc ttaaaaaggg tgttgaagaa ttaatcaata gaataaatga
     1081 aatgatattc aaggaagaaa gtataatgct tcctatgctt ttagatataa tgactcagga
     1141 tgaatggaaa aaggttgctg atgacagcag cgaaataggc aacataatag attttgtacc
     1201 tgtttggaat cctgataagg aagcaaaaaa ggagcataaa gaagatatta aggagccggg
     1261 agctataatt cttccgacag gtgtttttaa tacggaagag cttactcata tgctcaatac
     1321 tctgccaatt gatataacat ttgtggggag tgacgataaa gtaaaatatt tttcacaaag
     1381 cagtgaaaga atatttgctc gaacaaagac tattataggc aggaacgttt caaactgcca
     1441 cccgccggca agtgtacata tagtagaagg aattgtggaa gattttaagt caggcaaaaa
     1501 agaccacgag gatttctgga ttaaaatggg cgataaatat gtctatatac gatattttgc
     1561 agtcaggaat gaagaaggca agtatcttgg agttgtggaa gtaacccaga atattaagcc
     1621 tatacaggaa atcacgggag aaaaaagact ggtggaagac taatnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnctgaag gggataaagt cccctttttt attgtttttt
     1801 ggattagata agtatataat tgaaattaaa ggaaaagacg tgtccatgtt gaaaggggag
     1861 agttatggaa aagaaatatg ttcttgcact agaccaaggc acaacaagct caagggcaat
     1921 actatttgac agaaacggca gaatagtaaa tatatctcaa aaagaattca ctcaattata
     1981 tcccaatcca ggctgggttg agcacaaccc tttagaaata tgggaaactc agttaaatgc
     2041 tgcaaaagaa gcaataaaat atatagatgt ttctgaaatt gcatgcatag gtattaccaa
     2101 tcaaagagaa actacaattt tgtgggacaa aaatacaggt aagcccgtat acaatgcaat
     2161 agtatggcaa agcaggcaga cttccgatat ttgcgatgat ttaaaaaaat caggcttaga
     2221 accctacata aaagaaaata cgggattagt tattgatgct tatttttcag ggacaaaaat
     2281 aaaatggatt ttggacaatg tggaaggagc cagggaaaga gccgaaaagg gtgaaatatt
     2341 atttggaacc gtggatactt ggcttttatg gaatttaacg ggcggcaaag cacatataac
     2401 agattactcc aatgcttcaa gaactatgat ttacaatata aaaaatcttc ggtgggatga
     2461 aaaaattctt ggtatattgg atattcctag tgaaatactg cctgaagtaa ggcagtcatc
     2521 agaaatatat gctaatacca atgctgaaat tttcggtgca gaaataccaa tatcaggaat
     2581 tgcgggagac cagcaagctg ccctctttgg tcagctctgt tttcaggaag gcatggtaaa
     2641 gaatacatat ggtacgggat gctttatgct tatgaataca ggtgaaaaaa tagttcaatc
     2701 tcacaagggg ctcattacta caatcgcatg gggcttacaa gggaaggtcg aatatgcttt
     2761 ggaaggctct gtatttgtgg caggtgcagc aatacagtgg ttaagggatg agcttaaaat
     2821 aatacatgaa gcagctgatt ccgaatattt tgcaaataag gtcaaggata caaaaggtgt
     2881 ctatgttgtg cctgcattta cggggcttgg tgccccttat tgggatatgt atgcaagggg
     2941 agcaattgta gggttgacaa ggggagcagg cagaaatcat ataataaggg caactcttga
     3001 atcaattgct tatcaaacta aggatataat tgaagcaatg attgaagatt caggaatcaa
     3061 tttaacggta ttaaaggttg acggaggagc atcggcaaat aattttttaa tgcaatttca
     3121 gtcagatata cttaatgtaa atattgaaag acctgagatt acagaaacca ccgcacttgg
     3181 agccgcatat cttgcaggcc ttgcaacagg gttttggaaa tcaaaagggg aagttataca
     3241 aaactggagc atgaacaaaa agtttaaacc gtcaattaat gacgacgaga gaaatagatt
     3301 atata
//
LOCUS       DAOPUE010000171         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_30090_length_3261_cov_8.640674, whole genome shotgun sequence.
ACCESSION   DAOPUE010000171 DAOPUE010000000
VERSION     DAOPUE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30090_length_3261_cov_8.640674"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PKH42_09875"
     CDS             complement(<1..302)
                     /locus_tag="PKH42_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20784.1"
                     /translation="MSLLDRLEKQKFSGTGEKSAEITLYDEYSDLKEQVHKGFIEAVN
                     QQDISIFGVKGEQEEELLKIMDSLIESKASKISRAERSKLLKEIYNDVMGLGPLEA"
     gene            complement(327..1547)
                     /locus_tag="PKH42_09880"
     CDS             complement(327..1547)
                     /locus_tag="PKH42_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOA20785.1"
                     /translation="MKIKVLLISKMERNIEALRSKIEDEEIITIGDSIGGAQALDKIE
                     NTSPDIIIMTLGAGDTDVLNLAERIILHRPKTFVILVADSLDVEIMQEAVKVGCHNLI
                     EFPSTSKEFAEYIKSVYNNESIRIKSLSNKDTLVWASRVITVFGAKGGLGKSTIAANL
                     AVKLAEKRKKVALVDLDLQFGDLNIFLDLEPKDTIVELVQDVVTPNIDSVRSYMSVHS
                     SGVHVLCAPRSPEYAELVSADRVQSLLSLLRTYYDYVIVDTSPSFTDVTITAIESSSI
                     LFFVTGLDISILRNSKLSVSLLESLQQTDKIRLVVNRAVDMNSITINDVQKIIGYPIW
                     AKIPSDYKVAVNALNRGVPFVTGAPNSKLSASISGLTEILIKGTTDYDLMGKYKNNNN
                     NNNNKKKLGLFKKK"
     gene            complement(1560..2297)
                     /gene="cpaB"
                     /locus_tag="PKH42_09885"
     CDS             complement(1560..2297)
                     /gene="cpaB"
                     /locus_tag="PKH42_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOA20786.1"
                     /translation="MKKIKFLALVAAVIAAVLLYNFLNTLDEKASVEVVKTGVVIASA
                     NIPPNTPITDDMLEIKQLPVEAAHTEAVVNEAAAIGKFSKYEIMAGEQVLYTKLISPE
                     GGGGDGTLAYEIKPGMRAISIGVGNLSGLVGMIRPHNTVDVIAQLAREEKQADGEDKT
                     IDYTTMIAENVKVLAVDDVISQEGKKINTETGAAYTSVTLQVTTHQAMELNMSEYKGQ
                     LRLILRSPLDEELVKPSTIKFDDIIIN"
     gene            complement(2371..2802)
                     /locus_tag="PKH42_09890"
     CDS             complement(2371..2802)
                     /locus_tag="PKH42_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOA20787.1"
                     /translation="MKLKHFKKENGQAMVEFALVLPILLLFIAGILDFGWIYHNQLSA
                     NNASREAARYISIHYYSDNMNSTTATTKATNMIKEYTNITDIVVDTIIPENDYVNGGE
                     KIRVRFAGKVIILTPMARAIIDKDGDGKFPIRAETHMRVEK"
     gene            complement(2941..3120)
                     /locus_tag="PKH42_09895"
     CDS             complement(2941..3120)
                     /locus_tag="PKH42_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOA20788.1"
                     /translation="MKKIFSWLRNEESGQGMVEYGLILALIAVVVIVALQTLGSATGE
                     KLEEVGAAISGTSTP"
BASE COUNT          903 a          689 c          486 g         1183 t
ORIGIN      
        1 aatgcttcaa gaggtcctaa ccccattaca tcattgtaaa tttcttttaa taattttgag
       61 cgctcggctc gtgaaatctt ggaagctttg gactcaatta atgaatccat aattttaagc
      121 aattcttctt cttgctcgcc cttaacgcca aatatactta tgtcctgttg atttactgct
      181 tcaataaaac ctttatgaac ttgctctttt aagtcagaat attcatcgta caaagttatt
      241 tctgccgatt tttctccagt gccggaaaat ttctgttttt cgagacgatc caataaactc
      301 atgtcaattc ctgcctttta ttactgttat tttttcttga ataagccaag cttcttttta
      361 ttgttgttat tgttgttatt atttttatat tttcccataa ggtcgtaatc agttgtgcct
      421 ttaattaata tttctgtcaa accggaaatc gatgcgctca gtttactgtt tggtgcccct
      481 gttacaaatg gaactcccct gtttaatgca tttaccgcaa ccttgtaatc actgggtatt
      541 tttgcccata taggataccc tattattttt tgaacatcat ttattgtaat ggaattcata
      601 tcaactgccc tatttacaac taacctgatt ttatctgttt gctgcaatga ttccaacagc
      661 gaaacactga gcttggaatt ccttagaatt gaaatgtcaa gacctgtaac aaagaatagt
      721 atggaagaag attcaattgc agtaattgtt acatcagtga atgacggcga tgtatctaca
      781 attacataat cataataggt tcttaataaa cttaacaggc tttgtactct gtctgctgaa
      841 accaactcgg catattcagg gcttcttggt gcgcagagta catgaacacc gctcgaatga
      901 actgacatat agcttcttac ggagtcaata ttcggtgtaa ctacatcctg aaccagttcc
      961 acaatagtat cttttggctc taaatctaag aatatattca aatcaccaaa ttgtaaatct
     1021 aaatccacca atgcaacctt ttttcttttt tctgccagct ttacagctaa atttgctgca
     1081 attgtacttt tacccaatcc accctttgct ccaaatacag ttataaccct tgatgcccat
     1141 actaaggtat ctttattgct taatgattta attcgaatag attcattatt atatactgac
     1201 tttatatact ctgcgaattc ttttgatgtt gaaggaaatt caatcaagtt atgacaaccg
     1261 accttgacgg cttcctgcat aatttcaaca tctaaggagt cagcaaccag aataacaaat
     1321 gtctttggtc tgtgaagtat gattctttca gccaaattaa gtacatctgt gtctcctgca
     1381 cccaaggtca taataattat atcaggggat gtattttcaa ttttatctaa tgcctgtgct
     1441 cctcctattg aatcaccgat tgttataatt tcttcatctt caatcttgga cctgagtgct
     1501 tcaatatttc tttccatttt acttataagt aatactttaa ttttcataag ctgcccccac
     1561 taattaatta taatatcgtc aaattttatt gttgaaggct taactaactc ttcatctaac
     1621 ggagacctta agattaatct aagttgtcct ttgtattcgg acatattcag ctccatagcc
     1681 tgatgtgttg taacctgtaa tgtaacagag gtgtaggcag cgcctgtttc ggtattgatt
     1741 tttttacctt cttgagatat tacatcgtca accgctaaaa ccttaacatt ttcagctatc
     1801 atggttgtat aatctattgt tttatcttct ccgtcagctt gtttttcctc tcttgcaagc
     1861 tgggcaataa catccacggt attatgaggc ctaatcatac ccacaagtcc tgataaattg
     1921 ccaacgccta tagatattgc acgcatgccg ggctttattt catatgcaag agtaccatct
     1981 cccccaccgc cttcaggtga tataagctta gtgtataata cctgctcacc tgccataatt
     2041 tcatattttg aaaactttcc tatggctgcc gcttcattga caactgcctc tgtatgtgca
     2101 gcttcaacag gcagctgttt tatttcaagc atatcatctg ttataggtgt atttggagga
     2161 atattagcag atgctatcac aactccggtt tttactacct caacactggc tttttcatca
     2221 agagtattca ggaaattata taagaggaca gcagcaataa ctgctgcaac caatgctaaa
     2281 aattttatct ttttcataag ctcactccaa tttatatatt tgttaataag caaccaaatg
     2341 cagtccttac tccatatcat tcaatattat ttatttttcc actctcatat gtgtttcagc
     2401 tcttattgga aacttaccgt ctccatcttt atctattatt gctcttgcca ttggtgtaag
     2461 aataataacc tttcctgcaa acctgaccct tatcttttca ccgccattca catagtcatt
     2521 ttcgggtatt atggtatcta caactatatc agtaatattg gtatattctt taatcatatt
     2581 agtagcttta gtagttgcag ttgtagaatt catattatcg gaatagtagt gtatcgatat
     2641 ataccgagca gcctcacggc ttgcattatt ggcagacagc tgattatgat aaatccatcc
     2701 gaaatcaaga attccggcta taaaaagcaa cagtataggg agtaccaagg caaactcaac
     2761 cattgcttga ccattttctt ttttaaaatg cttaagtttc ataaactacc gctcccccct
     2821 ttgtgcctaa ttttttttat cttgtttttc ataggctgct ttacactatc ccattgtgga
     2881 aaaagcaccg tcccgaccat gcggacgagc cggtgctttt tgctggtttt tttggttaaa
     2941 tcaaggtgta gatgtgcctg aaattgcggc tccaacttct tctaattttt ctcctgttgc
     3001 acttccaaga gtttgtaagg ctactattac tactactgca atcaatgcaa gtatcaagcc
     3061 gtactcaacc attccttgac cgctttcctc gtttcttaac caactaaata tttttttcat
     3121 tttgaacccc cttgtattat ttatttatat tttgatataa aatatcataa tatacaattt
     3181 aatcttgtca tcccttttta cggtgacatc tctcagtctc gcatttcaat tttgtttgct
     3241 ccttccgtcg cacaaaattg g
//
LOCUS       DAOPUE010000172         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_30337_length_3242_cov_8.351428, whole genome shotgun sequence.
ACCESSION   DAOPUE010000172 DAOPUE010000000
VERSION     DAOPUE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30337_length_3242_cov_8.351428"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1128
                     /gene="hrcA"
                     /locus_tag="PKH42_09900"
     CDS             70..1128
                     /gene="hrcA"
                     /locus_tag="PKH42_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOA20789.1"
                     /translation="MSLDKRKVKILKAIISSYIDNAEAVGSRTISKKYELGVSPATIR
                     NEMSDLEDMGYLVQPHTSSGRIPTEKAYRYYVDDLWKKVKTSKNPKLDQLREIIEDKS
                     NELDEIFKNSVRILSQFTKYTSFVVAPQFKQSVIKRIHLVPVSEYKVLMLLILETNIV
                     KQVMLRLSSPIPFNQLEKISESLSEKMHGYRLEDIDKIKDNLIKELYYLRESNNDILM
                     DLLPYLVEQIAKFEDVNVYSDGITNILELPEYNDVVKAREFISFFEDKTSVARLMQYY
                     GENDLDIYIGSENKSQELRDCSLITATYKFNGKLIGRIGVIGPTRMDYNNVISTVKTM
                     SEAINEIIRLNFNGENKE"
     gene            1133..1738
                     /gene="grpE"
                     /locus_tag="PKH42_09905"
     CDS             1133..1738
                     /gene="grpE"
                     /locus_tag="PKH42_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOA20790.1"
                     /translation="MAKGKKHDEVNENVKNLEETEETMAESKEDTQRSDKTSETKEEK
                     VEEKPAEETENNELKKKNEEIEALNNKLLRLQADYLNYKDRVEKEKLTIYGDAVSAVI
                     CDLLPVIDNLERAISADVPDNFKEGVVMVYNQLMGILNKKGLKEIEALHKPFDHNLHY
                     GVAYEESEEFDDGIVIDVLQKGYTVNDKLIRPSMVRICKKS"
     gene            1849..>3242
                     /gene="dnaK"
                     /locus_tag="PKH42_09910"
     CDS             1849..>3242
                     /gene="dnaK"
                     /locus_tag="PKH42_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOA20791.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGEAVVIANVEGKRTTPSIVAFTK
                     DGERLVGETAKRQAVTNPDRTITSIKRDMGSNRKINIDGKTYTPQEISAFILQKLKAD
                     AESYLGEKVTEAVITVPAYFSDSQRQATKDAGKIAGLDVKRIINEPTAAALAYGIDKE
                     TQMQTIMVYDLGGGTFDVSILEIGDGVFEVKATSGNNRLGGDDFDQVIIDYLADQFQK
                     ENGVDLRKDKMSLQRLKEAAEAAKKELSSAMTTTINLPFITATQEGPKHMNMDLTRAK
                     FNELTSFLVEKTVEPVKKAMADAKLTASQIDKVLLVGGSTRIPAVQEAVKRLTGKDPH
                     KGINPDECVALGAAIQGGVLAGEFGTDILLVDVTPLSLGIETLGGVFTKLIDRNTRIP
                     TKKSQIFSTATDNQPAVDIHVLQGERQMAADNITLGRFQLSGIVPAPRGVPQIEVTFD
                     IDANGIVNVSAKDMG"
BASE COUNT         1278 a          512 c          674 g          778 t
ORIGIN      
        1 gtagtatctt aatattatgg agttagcact caaccaagga gagtgctaac aaagaaaagg
       61 agtgagaaaa tgtctttgga caagagaaag gtaaagattc taaaagctat tatctccagc
      121 tacattgata atgctgaagc tgtaggctca aggacaattt caaaaaaata tgaacttgga
      181 gtaagccctg ccaccatcag aaatgaaatg tctgatttgg aagacatggg ttatttagtt
      241 cagccacata cttcatcagg acgaataccg acagaaaaag catacagata ctatgtggat
      301 gatttatgga aaaaagtcaa gacttcaaaa aatcctaaat tagaccagct tagagaaata
      361 attgaggaca aatcaaacga attagatgaa atattcaaaa attcagttcg aatactgtcc
      421 caatttacta aatatacttc atttgtagtt gcaccacagt ttaagcaatc tgttattaaa
      481 agaatccatt tagtaccggt ttcggaatat aaggtattga tgctcttaat actggagacc
      541 aatattgtca aacaggtaat gctgagatta agcagcccca taccttttaa ccaacttgaa
      601 aaaatatccg aatcactttc cgaaaaaatg cacggctata gattagaaga tattgacaaa
      661 attaaggata acttaattaa agagctatat tatttgagag aatcaaacaa tgatatattg
      721 atggatttac tgccctattt agtggagcag atagcaaagt ttgaagatgt taatgtatat
      781 tcggacggta taacaaatat attggagctt cccgaatata atgatgttgt taaagcaaga
      841 gaatttatat cattctttga agataaaaca agtgttgcca gattgatgca atattacggc
      901 gagaatgatt tagatatata tataggcagt gagaataaaa gccaggaatt acgggattgc
      961 agcctcataa cagcaaccta taaatttaac ggcaagctaa tcggaagaat cggagtaata
     1021 ggaccgacta gaatggatta taacaatgta atttcaactg ttaaaacaat gtcggaagca
     1081 ataaatgaaa taattaggct aaactttaac ggagaaaaca aggagtgaag aaatggcaaa
     1141 aggtaagaag catgatgaag ttaatgaaaa tgtaaaaaat ttggaagaga cggaagagac
     1201 tatggcagaa tctaaagaag atacacaaag gtcagataaa acttctgaaa caaaagaaga
     1261 aaaggttgaa gaaaaaccgg ctgaagaaac agaaaataat gaattaaaaa agaaaaatga
     1321 agaaatagaa gcacttaaca ataaattgtt aaggcttcaa gcagactact taaactataa
     1381 agacagggta gaaaaagaaa agctcacaat atacggggat gcggtatcag cagtgatttg
     1441 cgatttgctt cccgtaattg acaaccttga aagagcaatt tcagcagatg tgccggataa
     1501 tttcaaggaa ggcgtagtga tggtttacaa tcagcttatg ggaattttaa ataaaaaggg
     1561 gttaaaagaa attgaagccc tgcacaaacc ttttgaccat aatctgcact acggagtggc
     1621 atatgaagaa agtgaagaat tcgatgacgg aatagtaatt gatgttcttc aaaaaggata
     1681 tactgtaaat gataaactca taagaccgtc aatggtcaga atatgtaaaa agtcgtaatc
     1741 ccttgtcatt ctgaacgaag tgaagaatct catgaagtcc ttcgttaaac tcaggatgac
     1801 aataaaatat agaaaatatt aaattcaaat cataaatgga ggaataaaat gggcaaaatt
     1861 ataggaattg acttaggtac aacaaactcg tgcgtatctg tgatggaagg cggagaagcc
     1921 gttgttatag ctaatgtgga aggcaaaaga accacacctt caatcgtggc atttacaaaa
     1981 gatggagaaa gattagtcgg agaaacagcc aaaagacagg cagttacaaa ccccgacaga
     2041 acaataacat caataaaaag agacatgggc tctaacagaa aaattaatat tgatggaaaa
     2101 acatatacac cgcaggaaat ctctgcattt atacttcaaa aattaaaagc tgatgctgaa
     2161 agctatcttg gagaaaaagt taccgaagca gttataacag taccggcata tttctctgac
     2221 agccagagac aggcaacaaa ggatgcaggt aaaatagcag gtttggatgt aaaaagaatt
     2281 ataaacgaac ctacggctgc agcccttgcc tatggtattg acaaagaaac acagatgcag
     2341 acaataatgg tttacgacct aggcggaggt acatttgacg tttctatact tgaaataggc
     2401 gatggcgtat tcgaggttaa agcaacatca ggaaacaaca gacttggcgg agacgacttt
     2461 gaccaagtga ttatagatta cttagcagac caattccaga aagaaaacgg tgttgactta
     2521 agaaaagata aaatgtcgct tcaaagatta aaagaagcag cagaagcagc aaagaaggaa
     2581 ttgtcaagcg caatgaccac aacaatcaat ctgcccttta ttactgctac acaagaaggt
     2641 ccaaaacata tgaacatgga ccttacgaga gcaaaattca acgaattaac atcattcttg
     2701 gttgaaaaaa ccgtagagcc cgttaaaaaa gcaatggcgg atgccaagtt aacagcttct
     2761 caaatagata aagtattatt ggttggaggc tccacaagaa ttcctgcagt acaggaagcg
     2821 gtaaaaagat tgacaggcaa agaccctcac aagggaatta accctgatga atgtgttgct
     2881 cttggagctg caattcaagg cggagtattg gcaggagaat ttggaacaga catactgctt
     2941 gttgacgtta cacctttgtc acttggaatt gaaacactag gaggagtatt tacaaaatta
     3001 attgacagaa atactcgtat acctacaaag aaaagccaga tattctcaac agcaacagac
     3061 aaccagcctg cagttgatat ccacgtattg cagggtgaaa gacaaatggc tgcagacaat
     3121 attacactag gaagattcca gttgtcagga attgttcccg caccgagagg agtacctcaa
     3181 atagaagtaa catttgacat agatgccaac ggtatcgtaa atgtaagcgc aaaggatatg
     3241 gg
//
LOCUS       DAOPUE010000173         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_30948_length_3197_cov_10.290898, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000173 DAOPUE010000000
VERSION     DAOPUE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30948_length_3197_cov_10.290898"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..318)
                     /locus_tag="PKH42_09915"
     CDS             complement(145..318)
                     /locus_tag="PKH42_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20792.1"
                     /translation="MINLFEIDLNMIASFAIVFGIIIYVGYVNTSIRELKKRVEELEN
                     KIGIDKKADEELK"
     gene            complement(409..1641)
                     /gene="tyrS"
                     /locus_tag="PKH42_09920"
     CDS             complement(409..1641)
                     /gene="tyrS"
                     /locus_tag="PKH42_09920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOA20793.1"
                     /translation="MEKGNVFDVLVERGYVEQCTHEEEIRELLGKESVTFYIGFDPTA
                     DSLHIGHFIQIMVMSIMQKYGHRPIALLGDGTTMIGDPSDRTGMRSVMSFETITKNAE
                     NFKKVFEKFLDFTDGKALMPRNSEWLLPLNYLEFMREVGVHFTISKMIAADCYKNRMD
                     QGLTFFEFGYMLMQAYDFYILNEKYDCKMQLGGNDQWSNIIAGVELTRKKAGKQVYGM
                     TFALLTNSEGKKMGKTEKGALWLDRNKTTPYEFYQYWRNIDDADVEKCLALLTFLPMD
                     EVRRLGALKGAEINKAKEILAFEITKMIHTEEDAVNAQEAARALFGGGADSENIPTTE
                     LTKAELGESISVIDLMLKAGLIKSKSEGRRLIEQAGVAVNDAIIDDIGATVTEADFED
                     SKLMIKKGKKTYHRIKLS"
     misc_binding    complement(1678..1874)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2219..3019
                     /locus_tag="PKH42_09925"
     CDS             2219..3019
                     /locus_tag="PKH42_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOA20794.1"
                     /translation="MKRRIIALTLALVIALSSTSAFAATTNCTSVNYKYNLKDFIKTN
                     TSNYANKNTQYKITVNGKTVDLKSIDWKTVLKQYAPAQKPAAPVEKPSPAPAPVEKPA
                     PKPAPVEKPAPAPAPVEKPAPAPAPAPVENNTGVPSSNLTYEQKVVELVNIERQKAGL
                     PALKMDSAISNVARTKSKDMAVNNYFAHQSPTYGSAGDMLRQFGISWRAWGENIAAGQ
                     RTPEIVVDAWMNSPGHRANILSSNFSKIGVGYATNSSGRPYWTQIFTN"
BASE COUNT          986 a          651 c          480 g         1080 t
ORIGIN      
        1 cttttcgtct aatatgagat tattttacca tactttccta aaactattaa taattatata
       61 taaaagaaat taaaaaagat cctccaatca gccttaggat acctaatcat caccccgaca
      121 gtcgtggaag atcttatatc attcttattt tagttcttcg tccgctttct tatctatacc
      181 aattttattt tccaattctt caactctttt ttttaactct cttatcgatg tatttacata
      241 tcctacataa attataattc caaacacaat agcaaatgaa gctatcatat ttaaatctat
      301 ttcaaataaa tttatcaaag aacatgcctc ctattgtatt atcgtattca aacactgagg
      361 cggtcccttt gtaatgaaaa ggaccgtcct caatggaaac agtatagatc atgacaattt
      421 aattcggtgg tatgttttct tgcctttttt aatcatcagc tttgaatctt caaagtcagc
      481 ttccgtaact gttgcaccta tatcatctat aattgcatca tttacagcaa ctccggcctg
      541 ctcgataagc cttcttcctt cgctttttga ttttattaat cctgccttaa gcattaagtc
      601 aataacagat atgctttcac ccagttctgc ttttgttaat tctgtagtcg ggatattttc
      661 tgaatcagct ccgcctccaa ataaagctct tgcagcttcc tgtgcattaa ctgcatcttc
      721 ttccgtatga atcatcttag ttatttcaaa tgcaagaatc tctttagctt tatttatttc
      781 agcgcctttt aatgctccca atcttcttac ttcatccata ggtaagaatg taagaagtgc
      841 caagcatttc tccacatcgg catcatcaat gtttctccag tactgataga attcataagg
      901 tgtggtttta ttcctgtcaa gccacaatgc tcctttttcg gttttcccca tttttttgcc
      961 ttcgctgttt gtcaataagg caaatgtcat gccataaacc tgtttgcctg ctttttttct
     1021 tgtcagttca acaccggcta ttatatttga ccattggtcg tttccgccca attgcatctt
     1081 gcagtcgtat ttttcattta aaatatagaa gtcgtaagcc tgcataagca tatagccgaa
     1141 ttcaaagaat gtaagaccct ggtccattcg atttttatag caatctgctg caatcatttt
     1201 gctgattgta aaatgtacac ccacttccct catgaattca aggtaattca aaggtaaaag
     1261 ccattcggaa tttcttggca tcagggcttt tccatccgta aaatcaagaa atttctcaaa
     1321 taccttttta aagttttctg catttttggt tatggtttca aatgacataa ccgagcgcat
     1381 tccggttctg tcactggggt ctcctatcat tgttgtcccg tcacctagta atgctatagg
     1441 tctgtgcccg tatttctgca ttattgacat taccattatt tgtataaagt ggcctatgtg
     1501 aagactgtcc gctgtcgggt caaatcctat ataaaatgtt acggattctt ttcccaataa
     1561 ttctcttatt tcttcttcat gagtgcattg ctccacatac cctctttcaa ctaatacatc
     1621 aaaaacattt cctttttcca tctattgccc tcctaactaa ataagggtta tttcatccga
     1681 aaggacgaga taacccgtgg taccacctta atttgcctaa gcctcttgct gatttaacgg
     1741 acagataagc cgctgtgctt ttcacacaga actccggaat cgtatttcac actttgtgct
     1801 gtgtatcttt caccacccga tacactctct aaattgtaac caaaatgtta cttaagttcc
     1861 ttcacagtca tgataaagta ttattggaat aataatataa aatttaagtg ttgtcaagaa
     1921 gtaaatttgt taattaaata caacccttac aaataatgcc ctaataatta accttttatt
     1981 aagtattttt gaataaacta tatatatttg tcataattat gtatttattt cttttgtttg
     2041 taaaatagtg taatattatt gacacatttg gcattataag aagttattga ttgttttttt
     2101 aggggaattg cagtgctata attctgatat ggcgaaaact cattaacaag ttctagcttt
     2161 tcaaaggaac aggccttacc tgtttcgaaa aaaatcttag actatatagg agtaaattat
     2221 gaaaagaaga attatagcat taacattagc gttagtaata gcgttatcat caacatcagc
     2281 atttgctgca acaacaaatt gcacatcggt aaattataag tataatctta aagattttat
     2341 caaaactaac acaagcaact atgctaacaa aaacacacag tataaaatta ctgttaacgg
     2401 aaaaacagta gatttaaaat caattgactg gaaaacggtt ttaaaacaat atgcaccggc
     2461 acaaaaaccg gcagcacctg ttgaaaaacc atctccggct ccggcacctg ttgaaaaacc
     2521 ggctccaaaa ccggctccgg ttgaaaagcc agctccagct ccagcaccgg ttgaaaaacc
     2581 agctccagct ccggctccgg cacctgttga aaataataca ggagttcctt cgtcaaattt
     2641 aacatacgaa caaaaagtag tagaacttgt aaatattgaa agacaaaaag caggcttacc
     2701 ggcattaaaa atggattcag caatttcaaa cgtggcaaga actaaatcta aagatatggc
     2761 agtaaacaat tattttgccc accagtcacc tacttatggc agtgcgggcg atatgttaag
     2821 acagtttgga atcagctggc gtgcatgggg tgaaaacatt gcagccggtc aaagaactcc
     2881 tgagatagtg gtagatgcat ggatgaattc acccggacac agagcaaaca tcttatcatc
     2941 taacttctca aaaattggag ttggatatgc aacaaactca agcggaagac catattggac
     3001 acaaatattc actaattaaa attaattaca atattaaaaa tgtgcaagac ttgactaagt
     3061 tttgcacatt tattttttta ttttggttta taaaataatt ttatctcttg ttatatcgct
     3121 taagtttttg caaccggtca ttatcattgt ttctcttaat tcagcaccga ttttttcggt
     3181 atacatttcc acacctt
//
LOCUS       DAOPUE010000174         3194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_30984_length_3194_cov_8.630137, whole genome shotgun sequence.
ACCESSION   DAOPUE010000174 DAOPUE010000000
VERSION     DAOPUE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30984_length_3194_cov_8.630137"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKH42_09930"
     CDS             complement(<1..194)
                     /locus_tag="PKH42_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOA20795.1"
                     /translation="MNFTTKETYLLEDQKNHEKICVQKYSSYANMASDPELKSIFQQN
                     GQKEQEHYNTLDQLLKGQMPS"
     gene            complement(329..1693)
                     /locus_tag="PKH42_09935"
     CDS             complement(329..1693)
                     /locus_tag="PKH42_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOA20796.1"
                     /translation="MEALMKINSAVNGIVWGPLIIVLIIGTGTYLSINTKFFSITKIG
                     YVLKNTLLKMFAKDDKGEGEVTAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WLAAIMGMTTKFAEVVLAVNYREKTPDGRFVGGPMYYITNGLGKNWKWMAVLFAFFGT
                     FASFGIGCMVQSNSVALSAQSTFGVPVMVTGIVLAILTGAVIIGGIKRIGAFTEKLVP
                     FMSAIYIVGGLWLIIANASQLPRAFSLIFTNAFTGTAAVGGFAGTVMMQAIRYGVARG
                     IFTNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFMDTIIICSITALSIMVTGVWESG
                     ESGAVLSAMAFDVGLPGVGKYIVSIGLILFAYSTVLGWEYYGERCLEYLLGTKPIMAY
                     RIIWVVAVVVGAVGGLTFMWDLADTLNALMAFPNLIGVLMLSPVVFRLTKEFFAAEKS
                     KANE"
     gene            1987..2571
                     /locus_tag="PKH42_09940"
     CDS             1987..2571
                     /locus_tag="PKH42_09940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOA20797.1"
                     /translation="MKIYDFTVKDMENNDISLSIYKGKVLLVINGATGCGFTPQYDGL
                     QKLYDKYSPEGFEILDFPSNQFLEQAPGTNEEIANFCRLNFGTEFKQFSKIEVNGPNE
                     EPLYRYLKTTAEEYRNQGTVDFYNKVKQYTPGIKENDIRWNFTKFLIDKEGNVVARFA
                     PNITPEEMEEHVLRLLRDEEIEIVCHSDFSNGCI"
     gene            complement(2641..>3194)
                     /locus_tag="PKH42_09945"
     CDS             complement(2641..>3194)
                     /locus_tag="PKH42_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HOA20798.1"
                     /translation="IRYGVARGIFTNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFT
                     DTIVICSITALSILVTGVWETGESGAVLSAMAFDTGIPVVGRFIVSIGLILFAYSTIL
                     GWEYYGERCLEYLAGTKPIIAYRLLWVIAVVVGAIGGLEFMWDLADTLNGLMAFPNLV
                     GVLILSPVVFKLTREFFAKERTH"
BASE COUNT         1053 a          641 c          548 g          952 t
ORIGIN      
        1 gacggcatct gaccctttaa tagctggtca agagtgttgt aatgttcttg ttctttttga
       61 ccattttgtt gaaaaatact tttcaattcc gggtctgatg ccatgttggc atagcttgaa
      121 tacttttgga cacaaatctt ttcatggttt ttttggtctt ctaaaagata agtttcttta
      181 gttgtaaaat tcatttaaac acctccaatt atattatttt caaaatatca aaatatatcc
      241 gttaaaaaaa caaaaaaaaa cgggcgttta aacatgttcg tttaaacgct cgtattaata
      301 tcatttttta gtgcttaaat actaattctt attcatttgc cttgcttttt tcagctgcga
      361 agaattcttt tgtcagcctg aatacaaccg ggctcagcat caggacacct atcaagttcg
      421 gaaatgccat caaagcgttc aatgtatctg ccaagtccca catgaaggtg agtccgccta
      481 cagctccaac tactacggct actacccaaa ttattctgta tgccattatc ggttttgttc
      541 ctaacaagta ttcaaggcat ctttcaccat agtattccca acctaaaact gttgagtatg
      601 caaacaatat aagaccgata cttacaatgt acttacctac acctggaaga cctacatcaa
      661 atgccattgc tgaaagaact gctcccgatt ctcctgattc ccaaacacct gtaaccataa
      721 tagaaagagc agtaatagag cagataatta ttgtatccat aaatacttcg aatactcccc
      781 acaagccttg acgtacagga tggtctgtag ttgcagcggc atgagctata ggagcgctac
      841 ccaagccggc ttcgttagta aagataccgc gagcaacacc gtaacggatt gcctgcatca
      901 taactgtacc tgcaaaacct ccaacggcag ccgtacctgt gaacgcatta gtgaaaatca
      961 atgaaaatgc acgaggtaac tgactggcat ttgctataat caaccaaaga ccgccgacaa
     1021 tataaattgc tgacatgaat ggaaccaatt tttcggtgaa agcaccgatt ctcttaatac
     1081 ctccgataat aacagctcct gttaatatgg ctaaaacaat acctgtaacc attacgggta
     1141 caccaaaagt agattgtgct gaaagtgcaa ctgaatttga ttgaaccatg cagccgatac
     1201 caaaggaagc aaaagtaccg aagaatgcaa ataaaacagc catccatttc cagttcttac
     1261 ccagaccgtt tgtaatgtag tacatgggac cgcctacaaa acggccgtca ggtgtttttt
     1321 ccctgtagtt tacagccaat acaacttccg caaattttgt tgtcataccc ataattgctg
     1381 caagccacat ccagaatact gctccggggc ctccaagtgc tatagctgtt gcaactcctg
     1441 caatattacc tgtaccaacc gtagcggcaa gagcagttgc aactgcctgg aatgcagtaa
     1501 cttcaccttc gcctttatca tccttggcga acatttttaa caaggtgttc ttaagtacat
     1561 agcctatttt agtaattgag aaaaatttcg tgttaattga caaataagtt ccggttccaa
     1621 taattaaaac tataattaga ggaccccata ctatgccgtt aacagcactg ttgattttca
     1681 ttaatgcttc caaacaaatc cctccattca aaatgttaga taaacgttta cattttagta
     1741 tttatgtatt agaacgatta aaataaagat taaataataa aataattaaa cgttttcaat
     1801 atatattata taacattata aaaataattc aactaaaatt attacaattt ttattatatt
     1861 tatttcatat ttaatttact atttttgatg gatattgtta aaattttttc aaacaaaaca
     1921 gtaaaaattt tgcttgaaaa taataaaagg gggtatcagt atattataaa tcaataggag
     1981 gataatatga aaatatatga ttttacagtg aaggatatgg agaataacga tatatctcta
     2041 tcaatttata aaggaaaggt attgcttgtg ataaacggtg caacaggttg cggttttaca
     2101 ccccaatatg acggacttca aaagctttat gataagtact ctcccgaagg gtttgaaatt
     2161 ttggactttc caagcaacca gttccttgag caggctcccg gtacaaatga agaaatagca
     2221 aatttctgca ggttaaattt tggaacagaa tttaaacaat tttcaaaaat tgaagtaaac
     2281 ggtcccaatg aggagccgtt gtacagatac ttaaaaacaa cagctgaaga atatagaaat
     2341 cagggaacgg ttgattttta caataaggtt aaacaatata ctcccggaat taaagaaaat
     2401 gatataagat ggaattttac caaattcctg attgataagg aagggaatgt tgtagcaaga
     2461 tttgcaccaa acattacgcc tgaagaaatg gaagagcatg ttttgaggtt gcttcgtgat
     2521 gaagaaattg agattgtgtg tcattcggat ttttcaaacg gctgcattta agggactgga
     2581 acagcaatac aaattgcatt agaaggcgct gagttgtcag cgccttgatt tctttcttct
     2641 ttaatgtgtc ctttcttttg caaagaattc tcttgtcaat ttaaatacta ccgggcttaa
     2701 tataagaacc cctaccaagt ttggaaatgc catcaatccg tttaatgtgt ctgccaagtc
     2761 ccacataaat tcaagtccgc ctatagctcc aactactacg gctattaccc atagcaatct
     2821 gtatgctatt attggctttg ttcccgcaag atattcaagg catctttcac cataatattc
     2881 ccatcctaaa attgttgagt atgcaaacaa tataagacct atacttacaa tgaatctacc
     2941 tactaccgga atacctgtat caaatgccat tgctgaaaga actgctcctg attctcctgt
     3001 ttcccaaacg cccgtaacca atattgaaag agctgtaatg gagcagatta ctattgtatc
     3061 cgtgaatact tcgaatactc cccacaaacc ttgacgaaca ggatggtcag tagttgcagc
     3121 agcatgtgct ataggagcac tacccaagcc ggcttcgtta gtaaagatac cgcgagcaac
     3181 accgtaacgg attg
//
LOCUS       DAOPUE010000175         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_32754_length_3071_cov_12.477785, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000175 DAOPUE010000000
VERSION     DAOPUE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32754_length_3071_cov_12.477785"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..874
                     /locus_tag="PKH42_09950"
     CDS             380..874
                     /locus_tag="PKH42_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOA20799.1"
                     /translation="MFNLGDKVVYPMHGAGVIETMESKEILGEIRNYYVLKMPIGEMK
                     LMIPVDNVNNIGLRNIIDKKTVEEVYNVLKQEAEINDSNWNKRYRDNMTKMKTGDIFE
                     VAQVVRDLTYRDKEKGLSTGEKKMLVSAKQMLISEITLSTGTDSGKVQEYMEEIINLD
                     ALEL"
     gene            1136..2230
                     /locus_tag="PKH42_09955"
     CDS             1136..2230
                     /locus_tag="PKH42_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HOA20800.1"
                     /translation="MIDKIVKAVIAAIGASLGAVIGVLLNNGGFLELSSLQNSLVILG
                     ISLVFGIIFFILSPKIKKTGLRIIEALEAEILKHSTMDIMLGALGLIVGFVIAFLISQ
                     PLSDIKYVGTIISVISFVFFGYLGLKVATRKKDDVLWPQLLNLRKSATVKKDKNKKDM
                     LSPKILDTSVIIDGRISDICKTGFLEGTLVIPEFVLKELRHIADSSDSLKRNRGRRGL
                     DILNILQKEDAVSVVIESKDYGDGIEVDVKLLKLAKELGGKVLTNDFNLNKVAEFQGV
                     EVLNINELANAVKPVVLPGEEMTVMIVKDGKETGQGLAYLDDGTMIVVEGGKKCIGDS
                     VVVTVTSVLQTAAGRMIFAKLKNAVNNKAV"
     gene            complement(2575..>3071)
                     /locus_tag="PKH42_09960"
     CDS             complement(2575..>3071)
                     /locus_tag="PKH42_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOA20801.1"
                     /translation="HSHVVGQLDYEAELAVIIGKICKNVSVEEAEGCIFGYTIANDIS
                     ARDIQTSHGQWHKGKSFDTFCPMGPYIAHRSNIPFPPELKIQSYVNKELRQNGNTSDL
                     IFDIPAIISDLTTGITLYPGDIILTGTPAGVGAGFNPPKYLKPGDTVDCLIEGIGTLS
                     NTIA"
BASE COUNT         1016 a          474 c          642 g          939 t
ORIGIN      
        1 gacggcagaa gaaattgtaa aacaggtaaa agaagcgctt aagatgccct aaccctattt
       61 ttcagagttt tttcatgaaa aatagcggtg ggtcatttgc aaaaacattc caaaatatgc
      121 tgccaaaagg tgcaaaatat tttagctaag gagactgccg gcaataaaaa atagaaaact
      181 ctgctcaaaa tgcaagagtg tcctcattta ggacaatctt atatttataa aaaaactgta
      241 aagaattcat taataaaatt taaaacaata tattaaaaat aagtgttttt attcttgaca
      301 agcatgcatt ataacgatat aataaacaat ttgacatttg ttgcttaatg aggtataatg
      361 tatagatagg aggtgcatta tgttcaattt aggcgacaaa gtagtatacc ccatgcacgg
      421 tgccggtgtt attgaaacta tggaatcaaa agaaattcta ggagaaatta gaaactatta
      481 cgtactgaaa atgcccatcg gggaaatgaa actgatgata cctgtggata atgttaataa
      541 tatagggtta agaaacataa ttgacaaaaa gacagttgag gaagtatata atgttctaaa
      601 acaggaagct gaaataaatg attccaactg gaacaaacgg tatcgggaca acatgacaaa
      661 aatgaaaaca ggtgatattt tcgaagttgc tcaggttgta agggatttaa cctacaggga
      721 caaagaaaaa gggctgtcaa caggagagaa gaagatgctt gtcagtgcaa agcagatgct
      781 tataagcgag attacacttt caaccggcac ggatagcggg aaggtacagg aatatatgga
      841 agaaataatt aatctggatg cattagaact gtaaataatt gctctaatgc attttttctt
      901 atgtcaactt gcttcattcc cctgtgtatg gctttctatg acctaaccca atttcttaga
      961 ctgctgacac tatggacctt atggccggca tgtcgcattt aagagaatat taataaattc
     1021 ttattataat atttcaacaa ttttttatag ccaaataata aaaaatatgt tacattatta
     1081 atgctatatt aaataatatt actattaatg ggtatactcg taaggaggtg attatatgat
     1141 tgataagatt gtaaaagcag ttattgctgc tattggggca tctttggggg cagttatagg
     1201 tgtattgctc aataacggcg gttttcttga attatcaagt ttgcagaata gtttagtaat
     1261 tttaggtatc agtttagttt ttggaatcat attttttatt ctttccccta aaattaaaaa
     1321 gaccggttta aggattattg aagctttaga agctgaaata ttgaaacatt ccactatgga
     1381 cataatgctg ggtgctcttg gacttatagt gggttttgta atagcatttc taataagcca
     1441 gcctctatcc gacattaagt atgtaggtac tataatttca gttatttcat ttgtgttttt
     1501 tggatatctg ggcttaaagg ttgctacaag aaaaaaggac gatgttttat ggcctcagct
     1561 gttaaatcta agaaagtccg caacggttaa aaaggataaa aataaaaaag atatgttgtc
     1621 tccaaaaatt cttgatacca gtgtaattat cgacggaaga atatctgata tatgcaaaac
     1681 aggattcctg gaaggaacgc tggttattcc tgaatttgtg ctgaaggaac tgagacatat
     1741 tgcagattcc tctgacagcc ttaaacgcaa cagaggaaga aggggtttgg atattttgaa
     1801 tatccttcaa aaagaagacg ctgtaagtgt ggtaattgaa tcaaaagatt acggcgatgg
     1861 cattgaagtt gacgtaaagc tattaaagct ggcaaaagaa ttaggcggca aggtgctgac
     1921 aaatgacttt aatttaaata aggttgcaga gttccagggt gtcgaagtgt taaatatcaa
     1981 tgagctggca aatgcggtaa aacctgttgt cctgcccggc gaagaaatga ctgtaatgat
     2041 tgtcaaggac ggcaaggaaa ccggtcaagg attggcctac ctggatgacg gcaccatgat
     2101 agttgtggaa ggcggcaaaa aatgcatagg tgattctgta gttgtaaccg ttacttccgt
     2161 cctgcagact gctgcaggaa ggatgatttt tgccaaattg aaaaacgcag taaataataa
     2221 agctgtttaa atgaatacat gaaaaaaatt taatatcagg agacacacct ttcggtgtgt
     2281 ctctttgcgt atgcaataac acaatttttg gcattcttct gtgaacacaa ttttcggtca
     2341 aatcctgtta agcgtatatt attataggtt ttcggtaaaa ataatcactt gattgtattg
     2401 ctttatgtaa ttgacgaaaa tgaatgccta cactcgaaaa tatcttttaa cgacaggatg
     2461 caggatgtgt caaatcaccg gcagaacatc ttattatagc aaatgcgtaa ttcaaagcct
     2521 gcgaaggcca ttttacagat aaatggaact aaagggtatt gtcatgttac ataactaggc
     2581 tattgtgttt gaaagtgttc ctattccttc aataaggcag tccactgtat ctcccggctt
     2641 caggtatttt ggaggattaa atcctgctcc tacaccggca ggagtacctg tcagtattat
     2701 atctccggga taaagggtta tacctgtggt aaggtcgctt atgattgcag gaatatcaaa
     2761 tatcaagtca cttgtattgc cgttttgtct aagctcttta tttacatagc tctgaatttt
     2821 taattccgga gggaagggta tattggacct gtgtgctatg tatggtccca tagggcagaa
     2881 tgtatcaaag gactttccct tgtgccactg gccgtgtgaa gtttggatat ctcttgcgga
     2941 gatatcattg gcgattgtat atccgaatat gcagccttca gcttcctcca ctgagacatt
     3001 tttgcatatt ttgccaatta ttactgctaa ttctgcttca tagtccaatt gtccgacaac
     3061 atgtgagtgt g
//
LOCUS       DAOPUE010000176         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_33519_length_3025_cov_9.564983, whole genome shotgun sequence.
ACCESSION   DAOPUE010000176 DAOPUE010000000
VERSION     DAOPUE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33519_length_3025_cov_9.564983"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="PKH42_09965"
     CDS             complement(<1..97)
                     /locus_tag="PKH42_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOA20802.1"
                     /translation="MANIKSALKRIKVANFKTRRNRIIVSSLKTSI"
     gene            complement(419..1873)
                     /locus_tag="PKH42_09970"
     CDS             complement(419..1873)
                     /locus_tag="PKH42_09970"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HOA20803.1"
                     /translation="MIDIIVDEKDLSFNRLEKEIYAFGCKVACEVLKNVLETMDKKLA
                     KERDKAKYRHKGSRKTTVKTLMGEVAFSRAVYKSIGESSKEKYIYLLDQLLGFDAIGL
                     ISTNLAERIVENASIASYRNAADNITELSGQSISHSGVWNVVQALGQKIREEETELAK
                     AAEKNQLRGEKEVPVLFEEADGVYINIQGKDRPKSGKKLEMKVAVAYEGWKETRKNRY
                     ELVNKVACAGFEDISEFYRIKEAMIAREYNVDEIQMRILNGDGAAWIKHGIDDTVHYQ
                     LDPFHKHKAILKSVRDGEQRKTILKLLNSNEINEALAYIAGLANDTEDEKEQKKLKDL
                     YSYFKENRDGLIPYQERGLDIPKPPLGIEYRNLGTMEHHICDIIAQRMKHRKASWSIE
                     GASNLGKLLAAKASRRLNEVVEKFSKIVLSEEKTYEIIEILSSSKSPKKDGKGKDGNI
                     HKGQRPFTNYPVTNGRKAIRNMFDMRNFTDLIYR"
     gene            complement(2041..2790)
                     /gene="arr"
                     /locus_tag="PKH42_09975"
     CDS             complement(2041..2790)
                     /gene="arr"
                     /locus_tag="PKH42_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HOA20804.1"
                     /translation="MNIRFDPNNVVIKLCMSGMNLEDGGNIEDAATMFHKAWHESTDD
                     YERFIAAYFLARRQKNTADKLKWMETSLQCALNINDDNVKSAYPTLYLNIAECYAELC
                     DSDNAKRNYELSNSYRGAPSDEGPFYHGTKADMQVGDLLTPGGNSNYKPELKMNHIYF
                     TANANGAGLAAALAKGEGRERVYIIEPTGEFENDPNVTDKKFPGNLTRSYRSQEPLRI
                     IGEETEWVKLTTAERSEWRENLAKNKGEIIN"
     gene            complement(2805..>3025)
                     /locus_tag="PKH42_09980"
     CDS             complement(2805..>3025)
                     /locus_tag="PKH42_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20805.1"
                     /translation="LLISKELADSNAMKHYTKEDLEKALAPISSLINKSEKARKKLAE
                     GTWQHTMLSNNLKALYIASRLFTKALSG"
BASE COUNT          748 a          697 c          445 g         1135 t
ORIGIN      
        1 ttattgaagt tttcaaggag gaaacaatta ttctatttct tcttgtcttg aaattcgcta
       61 cttttattct ttttaaagct gatttaatat ttgccaaacc cttcacctcc ttcaaaattc
      121 aattgacaaa aagcattcta tcatattaac caaaaaaatg caagtgcatt gtattgagcg
      181 ttactgtcaa gtaaacgttg gcaactttcc ttcatacaaa ttttttgtaa atgtatgtgc
      241 aaatttggtc gcatttattc cacgatccta ttgacatggg gtcccctgta ccctctggac
      301 acctgttttt aggcggaatt agcctgtatt ttatgcgact tccgccgcca aaattttacc
      361 agagggtacc accccatgta aataggcttt cgcggcataa acgctctgtt tagttatatt
      421 acctgtaaat taaatcggtg aaatttctca tgtcaaacat attgcgtatt gctttcctcc
      481 cattggtaac tggataattc gtaaatggtc tttgcccctt atgtatattc ccatctttac
      541 cttttccatc ttttttcggt gattttgatg aacttaatat ctcaattatt tcatatgttt
      601 tttcttccga taatacaatc tttgaaaact tttcaacaac ttcattcagg cgtctgcttg
      661 ccttagcagc taataacttc cctaaattac tggctccttc aatactccag cttgctttac
      721 gatgtttcat cctctgggct attatgtcac atatgtgatg ttccatagtc ccgagattgc
      781 ggtactctat ccctaaagga ggctttggaa tatctaaccc tctttcctgg taaggtatta
      841 agccgtctcg gttctcttta aagtatgagt ataaatcctt aagcttcttt tgctctttct
      901 catcttctgt atcatttgct aatcctgcaa tatacgctaa agcctcatta atttcgttgc
      961 tatttaatag tttaagtata gtctttcttt gctctccatc ccttacactt ttgagtattg
     1021 ccttatgttt atgaaaagga tccagctgat aatgtactgt atcatctata ccatgcttta
     1081 tccaggcagc tccgtcacca ttaagtatcc tcatttgtat ctcatcaaca ttgtattctc
     1141 ttgctatcat tgcttctttt attctataga attcagatat atcttcaaac cctgcacagg
     1201 ccaccttgtt taccagttca tatctattct ttctggtttc tttccaccct tcgtatgcca
     1261 cagctacttt catttcaagt ttcttacctg acttcggacg gtctttacct tgtatgttta
     1321 tatacacccc gtcagcttct tcaaaaagaa caggtacctc tttttcccct cgcagctgat
     1381 tcttctctgc tgctttagcc agttctgttt cttcttccct tatcttttgc cctaaagcct
     1441 gaactacatt ccatacacca ctatggctga tactttgtcc gcttagctct gttatgttat
     1501 cggcagcatt acggtaggat gctatgcttg cattctcaac tattctctct gccaggtttg
     1561 ttgatatcag tcctattgcg tcaaacccca aaagctgatc cagtaagtat atatattttt
     1621 ccttgcttga ctcaccaata cttttatata ctgcacggga aaaagctact tcccccatca
     1681 gcgtcttcac tgttgttttc ctactccctt tatgtctata ctttgcttta tccctttctt
     1741 tcgcaagctt cttatccatc gtttctaata catttttgag tacttcacag gccaccttgc
     1801 aaccaaaagc ataaatttcc ttctctaacc tattgaatga taaatccttt tcatctacaa
     1861 ttatatcaat catacaaatc actcctgtcg atgttttctt gacaattaca ttctacagtt
     1921 tgatttgtat gacgggaaga ggtttttgtc tcttcccttt attttgtcat ttattttgcc
     1981 aattaaaatt atactctaag atgaaaattt tgctatttac gacgcgagcc gcacccatgt
     2041 tcagttaata atttcgccct tatttttagc taaattttcg cgccattcac ttcgctccgc
     2101 agttgtcagt ttcacccact ctgtttcttc accgataatt cttaaaggct cttgagagcg
     2161 ataagaacgt gtcaagttac cgggaaattt tttgtcagta acatttggat cgttctcaaa
     2221 ttcacctgtt ggttctatta tataaacgcg ttctcttcct tctccttttg ctaatgctgc
     2281 tgcaagtcct gcgccattgg catttgcagt gaaataaatg tggttcattt taagctcagg
     2341 tttgtaattt gaatttccgc ccggtgtcag taaatcacca acctgcatat ctgcctttgt
     2401 cccatgataa aatggtcctt catcagaagg cgcaccccta tatgaatttg acaattcata
     2461 atttcttttt gcattatcag aatcacacaa ctccgcataa cattcggcaa tgtttaaata
     2521 taacgttgga tatgcactct taacattatc atcatttata tttagtgcac actgtaaaga
     2581 tgtttccatc catttcaatt tgtccgcagt attcttttgt cggcgagcta aaaaataagc
     2641 tgcaataaat ctttcatagt cgtctgttga ttcatgccat gctttatgaa acattgtggc
     2701 tgcgtcttca atgtttccgc catcttctaa attcatcccg ctcatacaga gcttaataac
     2761 gacgttattt ggatcaaatc ttatattcat ctgatcttgc ctccctaccc gctcaatgct
     2821 ttcgtaaaaa gccgtgaagc tatataaagt gctttaagat tattactcag catagtatgc
     2881 tgccaggtgc cctccgctag ttttttccgt gctttctcgg atttgttaat cagggaagat
     2941 attggcgcaa gcgctttttc taaatcttcc ttggtataat gtttcattgc attactatcc
     3001 gccagttctt ttgatatcaa taatg
//
LOCUS       DAOPUE010000177         2847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_36476_length_2847_cov_10.569484, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000177 DAOPUE010000000
VERSION     DAOPUE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36476_length_2847_cov_10.569484"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..747
                     /gene="udk"
                     /locus_tag="PKH42_09985"
     CDS             88..747
                     /gene="udk"
                     /locus_tag="PKH42_09985"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOA20806.1"
                     /translation="MKKPIFIGVSGGTGSGKTTIVNRICSEVPEKSVAIMEHDSYYKD
                     QSSMTYEERCQTNYDHPFAFDTDLFVEHIKELKKGNSIQKPIYDYSIHNRKKETIIVE
                     PKEIIIVEGLLVFYEERIRELLDIKIYVDTDADIRILRRIIRDMNERSRSLDSVITQY
                     LNTVRPAHEQFIEPSKKYADIIVTEGGNNLVAVDLMVTKIKSLLNMSNSDTITHGANI
                     D"
     gene            740..1288
                     /gene="rnmV"
                     /locus_tag="PKH42_09990"
     CDS             740..1288
                     /gene="rnmV"
                     /locus_tag="PKH42_09990"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HOA20807.1"
                     /translation="MIKEIIVVEGKADVSAVKRAVDAQVISTNGLGINDKIINVIKKA
                     SKNKGIIILTDPDYPGKKIRNILASQIENCKHAFIPRDKANKDGDIGVENASKEAIIE
                     ALKNARAEVAENRQEFSYQDMVRYGLVGNDNASKRRSAIGDELGIGYCSAKQFLKRLN
                     SFGISREELEDAIKKTIEDING"
     gene            1281..2150
                     /gene="rsmA"
                     /locus_tag="PKH42_09995"
     CDS             1281..2150
                     /gene="rsmA"
                     /locus_tag="PKH42_09995"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOA20808.1"
                     /translation="MDEIKLYSPKVIKDILDRYGFKFSKSLGQNFLIDGNIIKRIVEI
                     AGLDEKSGVLEIGPGFGTLTQLLCKKANKVIAIEVDKSLIEVHKGTLDYPNLKIIYDD
                     FLKVDVNKLIEEEFKGLDVKIVANLPYYITTPIIMKILEEKYKVSKIVVMVQKEMAQR
                     LNSKPGTKNYGAITLAVQYRADTNIAMIVPNSVFMPKPKVDSAVIEFRILPKPRIDVC
                     DEKMLFKVIKASFAQRRKTILNGLSNSLNFSKDIINESLVSAGMNPGIRGEKLTLDEF
                     GRISDEISKRVKD"
     gene            2159..2707
                     /locus_tag="PKH42_10000"
     CDS             2159..2707
                     /locus_tag="PKH42_10000"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOA20809.1"
                     /translation="MKIYTKTGDKGQTSLYDGTRVDKDSLRVESYGTVDELNSYIGFA
                     MKFVNDESINESLKKIQMRLFFVAGELATVEEGKYKYKVKEEDIAALEKIIDDYLPKI
                     SGVDKFIVPGSSEASAALHVARTICRRAERRIISLKRHEEVSDILLKFVNRLSDVLYT
                     YARFLESNLTIMDFEKAYNEKL"
BASE COUNT         1070 a          366 c          604 g          807 t
ORIGIN      
        1 tatttttttg ttatgattta tttgtatatg gtaaaatagg aaaaaatgtt gtatattatt
       61 attataataa acatttgaga ggaaattatg aagaaaccaa tttttatagg ggtatcgggt
      121 ggaaccggtt ccggtaaaac aacaattgta aatcgaattt gctcagaagt tcccgaaaaa
      181 agcgtagcaa ttatggagca tgattcttat tataaagacc aaagttcgat gacttatgaa
      241 gaaagatgtc aaacaaatta cgaccatcca tttgcatttg atacggattt gtttgttgag
      301 catattaagg aattgaaaaa aggaaattca attcaaaaac ctatttatga ttacagcata
      361 cataacagga aaaaggaaac aattattgtt gagccaaagg aaataataat tgtggaggga
      421 cttttggtat tttatgaaga aagaataaga gaacttctcg atattaaaat ttatgtcgat
      481 acagatgcag atataagaat tcttagaaga attataagag atatgaatga aaggagcaga
      541 agtcttgatt ccgttattac ccagtattta aatacggtaa ggcctgctca tgaacagttt
      601 atagagccaa gtaaaaaata tgctgatatt attgtaaccg aaggaggcaa taatttagtg
      661 gcagttgatt tgatggttac aaaaataaag tctttgttaa atatgagcaa ttcggatact
      721 attacacatg gagcgaatat tgattaagga aattattgtg gttgaaggca aggcagatgt
      781 ttcggcagtc aagagggctg ttgatgcaca ggtgatttca accaatggat taggtataaa
      841 tgataaaatt attaatgtta ttaaaaaggc atcgaaaaac aagggaatta ttatactgac
      901 cgaccctgac tatccgggaa agaaaataag aaatattctt gcttcccaaa tagagaactg
      961 caagcatgca ttcataccca gggataaggc aaataaagac ggggatatag gagttgaaaa
     1021 tgcttcaaaa gaagcaataa ttgaagcgct gaaaaatgca agggctgaag tcgcagaaaa
     1081 cagacaggag ttttcttacc aagacatggt gcgctatgga ttggttggga atgataatgc
     1141 atctaaaaga agaagtgcca taggtgatga attaggtata ggatattgca gcgcaaaaca
     1201 atttttaaag aggcttaatt ccttcggtat aagcagggaa gagttagaag atgcaatcaa
     1261 aaaaacaatc gaggatatta atggatgaaa taaagctgta ttcaccaaaa gttattaagg
     1321 acatacttga caggtacgga tttaaattca gcaagagtct gggacagaat ttccttattg
     1381 acgggaatat aataaaaaga attgtagaaa tagccggttt ggatgaaaag tcgggagtat
     1441 tggaaatagg accgggcttt ggcaccttga cccagctttt gtgcaagaaa gcaaacaagg
     1501 taattgccat agaggtagat aaaagcttaa tagaagttca taagggaaca cttgattacc
     1561 ccaacttaaa aattatttat gatgactttt tgaaggttga tgtaaataaa ttaattgaag
     1621 aagaatttaa ggggcttgat gtaaaaatag ttgcaaattt accttattac attactacac
     1681 ccatcattat gaaaatattg gaggaaaaat ataaagtttc aaaaattgtt gtaatggtac
     1741 aaaaggaaat ggctcaaagg cttaattcaa aacccggaac caagaattat ggtgctatta
     1801 ctcttgccgt tcagtacagg gcagacacaa atattgcaat gatagttcca aattcggtat
     1861 ttatgccaaa accaaaagtt gattctgctg ttattgagtt tagaatttta ccgaaaccga
     1921 gaatcgatgt atgtgatgaa aagatgcttt ttaaagtgat taaagcttca tttgctcaaa
     1981 gacgaaagac aatacttaac ggattaagca atagtttaaa tttttctaaa gacataataa
     2041 atgaatctct ggttagtgca ggcatgaatc cgggtataag aggagaaaaa ctaacactgg
     2101 atgaattcgg aagaatatcg gatgaaataa gtaaaagagt aaaagattag gaggaattat
     2161 gaaaatttat accaagaccg gtgataaagg tcaaacatct ttatatgacg gcacaagagt
     2221 agataaggat tcattaagag tggaatcata cggaacggtt gacgaattaa attcttatat
     2281 cggatttgct atgaaatttg ttaatgatga aagtataaac gagagcctta agaaaattca
     2341 aatgcgctta ttttttgtgg cgggagaatt ggctaccgtt gaagaaggga aatacaagta
     2401 taaggttaaa gaagaagata tagccgcatt ggaaaaaata atagatgatt atcttcctaa
     2461 aataagcgga gtcgataaat ttatagtacc cggctcttcg gaagcttcag ctgcacttca
     2521 tgttgcaaga acgatttgcc gcagggctga aaggagaatt atatctttaa aacgccacga
     2581 agaagtaagt gacatacttt tgaagtttgt aaacaggctg tcggatgtat tatacacata
     2641 tgcaagattt ttagaaagta atttgaccat aatggatttt gaaaaggctt ataatgaaaa
     2701 actttagaat acactattat gagatccttc gcttcgctca ggatgacaaa gcgtattcat
     2761 atattgcagg gtgtcattga tattagtagc ccatggaatg tcattctgag agctttagct
     2821 cgaagaatct catgagatcc ttcgctt
//
LOCUS       DAOPUE010000178         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_37370_length_2797_cov_12.407002, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000178 DAOPUE010000000
VERSION     DAOPUE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37370_length_2797_cov_12.407002"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..953)
                     /locus_tag="PKH42_10005"
     CDS             complement(324..953)
                     /locus_tag="PKH42_10005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOA20810.1"
                     /translation="MTKIYFTRHGETVWNRQFRFQGHKDSDLTDKGVLAAELLADRIE
                     EIEMDYIISSPLMRAYSTAEILKGKKDVEIIKHDGLKEINLGDFEGMSYTDIKKEHTE
                     LLTEIEKDPFNNRYPNGENLREFYNRVVDAFKDIIENYRNKTTLIVAHGGTIKCIEAY
                     IRKFKLDSDWIGNVVKNCSLSYIEVDEHNEIKEIFYNDTKHLEGSAVLN"
     gene            complement(1035..>2797)
                     /gene="ligA"
                     /locus_tag="PKH42_10010"
     CDS             complement(1035..>2797)
                     /gene="ligA"
                     /locus_tag="PKH42_10010"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOA20811.1"
                     /translation="PKERHEKAMLSLDKTKDLSVLKDWLGEKTGLLSWKLDGLTIVMT
                     YEGGKLVKAVTRGNGEIGEVVTNNARVFINIPVSIAYKGSLVLRGEAVISYSDFNKLN
                     EQIPEAEGKYKNPRNLCSGSVRQLNNKITAERNVRFYAFSLVKADVDFKNSRIEQLNW
                     LNSQGFSAVEFKKTTREDIEERVKWFEDHISGNDLPSDGLVLTYDDIEYGSSLGTTAK
                     FPRDSIAFKWRDEIKETRLLEIEWSASRTGLINPIAIFEPVDLEGTTVSRASVHNLSI
                     MEGLELGIGDTIKVYKANMIIPQVSDNLTRSNNIEIPENCPVCSGETIIKNENDIKTL
                     YCVNDECLAKQIKSFTHFVSRDALNVEGLSEATIEKLIAKGVVKELADIFHIEKFKDI
                     ITNMEGFGEKSFNNLVNSVNKARKTTAARLLYSFGIANIGLSNAKIISRRFSDDWKRI
                     QGAAYDDLVEINGIGEVMAKNYVKFFSDEKKKVIVEDVLKEVEIEVSPKTSLSQVFKN
                     FIFVITGSVDHFKNRDELKAAIEERGGKVTSSVTSKTNYLINNDNLSNSSKNKKAKEL
                     GIEIITEEQFLQWMNTGEKE"
BASE COUNT          734 a          607 c          411 g         1045 t
ORIGIN      
        1 cttcttcacc gtattctctg aaatatgaag tgttgtacgg gcagcagaac atttccgaaa
       61 acacagccat gtctgcacct tcctttgaaa cacgttctat catatttaca gcatttttaa
      121 tattttttac tttatcctca taaaccatgg attgtattaa tgcaatttta aacttcataa
      181 gcaacctcct gcttctaata gtcaatattc tgcttttttc tgtttttccc gggaaatttc
      241 gacataattc tatctgttgc tgaaaaatat cataacaggg gaacgcaggg gaatgcaaca
      301 aaaaatataa gtgttatgac atactaattt agtacagcgc ttccctcaag gtgttttgtg
      361 tcattataga aaatttcttt tatttcatta tgttcatcta cttctatata tgaaaggctg
      421 cagtttttaa ccacatttcc aatccagtcg ctgtcaagct taaattttct tatatatgct
      481 tctatgcatt taatggtgcc accgtgggca actatcaatg ttgttttgtt tctgtagttt
      541 tcaattatgt ctttaaatgc atcaacaact ctattgtaaa attcccgcaa attttcacca
      601 tttgggtagc ggttgttgaa agggtccttc tcaatttcag taagcagctc agtatgttct
      661 tttttgatat cagtatagct cataccctca aagtcaccca aatttatttc ttttaaacca
      721 tcatgtttta ttatttccac gtcctttttt ccctttaata tttcagcagt gctgtaagct
      781 ctcatcaaag gactggaaat tatatagtcc atttcaattt cttcgattct gtctgccaaa
      841 agctctgctg ccagaactcc cttatctgtc aggtccgaat ccttatgtcc ttgaaaccta
      901 aactgtctgt tccaaactgt ttccccgtgt ctggtaaaat atatttttgt cataataaat
      961 gtgcgtcccc ttccttgaat ttctgctatt tcttctatgg tctattgtat catgaatttc
     1021 tttaataatt ccccctattc tttctcgcct gtattcatcc actgaagaaa ctgctcttca
     1081 gttataattt ctatccctaa ttccttcgct tttttatttt ttgaggaatt tgataaattg
     1141 tcgttattaa tcaaataatt tgtttttgaa gttacagatg atgtaacctt tccgcccctt
     1201 tcctcaattg ctgcttttaa ttcatccctg tttttaaaat ggtctacaga ccctgtaatt
     1261 acaaaaataa aatttttaaa aacctgtgac aaagatgttt tagggctgac ttcaatttcc
     1321 acctctttaa gcacatcttc cacaatgact ttcttttttt catcgctgaa gaattttaca
     1381 taattcttag ccattacttc accgatgccg tttatttcaa ccaggtcatc atatgcagct
     1441 ccttggattc tcttccagtc atcgctaaat ctcctgctta ttattttggc atttgacaag
     1501 cctatatttg caattccaaa gctgtataaa agtcttgccg cagtggtttt tcttgcctta
     1561 tttactgaat taaccaggtt gttgaaagat ttctcgccaa atccttccat attggtgata
     1621 atatctttga atttttcaat atgaaaaata tcagctaatt ccttaactac tccttttgca
     1681 attaattttt ctatggtagc ttcagatagg ccttccacat ttaatgcatc tctgcttaca
     1741 aaatgagtga atgattttat ctgctttgcc aggcattcat cattgacaca gtatagtgtt
     1801 ttaatgtcat tctcgttttt aattatggtt tcaccgctgc aaacaggaca attttcggga
     1861 atttcaatat tattgcttct ggttaaattg tcagaaacct gtggaattat catatttgcc
     1921 ttatatactt tgattgtatc gccgattcca agctccaagc cctccataat gcttaaattg
     1981 tgaacgctgg cacggctaac ggttgtccct tcaaggtcta caggttcaaa aattgcaatt
     2041 ggatttatca atcctgtcct tgaggcactc cattcaattt ccaaaagtct ggtttccttt
     2101 atttcgtctc tccatttaaa ggctattgaa tctcttggaa actttgcagt ggtccccaat
     2161 gagctgccgt attctatatc gtcatatgtc agcacaaggc cgtcagaagg aaggtcattg
     2221 ccgctaatat ggtcttcaaa ccacttaact ctttcttcta tatcttccct ggttgtcttt
     2281 ttaaattcaa ctgcgctgaa tccttgactg ttaagccagt taagctgctc aatccttgaa
     2341 ttcttaaagt caacatctgc cttaacaagg gaaaaagcat aaaaccttac atttctttcc
     2401 gcggttattt tattgttaag ctgccttacc gagccgctgc aaagatttct cggattcttg
     2461 tatttgcctt cggcttcagg tatttgttca ttgagcttat taaaatcaga atagcttata
     2521 actgcttctc ctcttaatac cagactgcct ttatatgcta tgctgaccgg aatatttata
     2581 aaaaccctgg cattgttagt aacaacttcc ccgatttctc cattgcctct ggttacagcc
     2641 tttacaagct ttcctccttc ataggtcata acaattgtaa gaccgtcaag cttccatgaa
     2701 agaagacctg tcttctctcc cagccagtct ttcaaaacag aaaggtcttt tgttttatcc
     2761 aatgaaagca ttgccttctc atgtctttcc ttgggca
//
LOCUS       DAOPUE010000179         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_37408_length_2795_cov_7.849270, whole genome shotgun sequence.
ACCESSION   DAOPUE010000179 DAOPUE010000000
VERSION     DAOPUE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37408_length_2795_cov_7.849270"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..555)
                     /locus_tag="PKH42_10015"
     CDS             complement(37..555)
                     /locus_tag="PKH42_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOA20812.1"
                     /translation="MTSEERRARIEEILKNSTEPVTASYFAKKFSVSRQVIVGDIALM
                     RAGGLKITATPRGYVFDKEEESKCVFTVACCHDEKNLGDELFAIVDNGGTVQDVIVEH
                     PIYGEIIGELHIRSRYDVELFLKKIAEKKVQPLLRLTDGIHLHTIKCKDIETKNRIIN
                     VLKLKNIMLEQK"
     gene            complement(638..711)
                     /locus_tag="PKH42_10020"
     tRNA            complement(638..711)
                     /locus_tag="PKH42_10020"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Pro,seq:cgg)
     gene            905..1519
                     /locus_tag="PKH42_10025"
     CDS             905..1519
                     /locus_tag="PKH42_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20813.1"
                     /translation="MYDRYMPYLANNKMLDLTYKKLKSLESPQIGYLKLNTYRHDTRS
                     PISNAFVRVSKLTISGYYREIGTGVFLAANSSDENGSVPIFELPVLTNENEMYNVSVN
                     YLDYCPAYIIDIPIYPGVTTTYDVYMHHYSMNGDVDMDYHLFCSRKYRAEIQDPKYFK
                     SGVILSMCRKMSYNCKMTAAEALHILFGQLNEQKDFLTRLLIYP"
     gene            complement(1401..>2795)
                     /locus_tag="PKH42_10030"
     CDS             complement(1401..>2795)
                     /locus_tag="PKH42_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOA20814.1"
                     /translation="EFFYSKINLSKVSSPKLVVLNNELAEYLGLDINELQSEEGVSYF
                     AGNSVPKGSIPIAQAYAGHQFGYFTMLGDGRAMLLGEQITPNGKYDIQLKGSGRTPYS
                     RGGDGRAALGPMLREYIVSEAMHALDIPTSRSLAVVTTGEPVIREKILKVAILTRVAS
                     SHIRVGTFEYAANFGTEENVRQLADYTIDRHYKEIEKDDFPYLSFLKEVVKRQASLIA
                     KWQLVGFIHGVMNTDNMALSGETIDYGPCAFMDAYNPDTVFSSIDTYGRYAYKNQPKI
                     AVWNLSRFAETLLPLLHEKNDKAIKLAQDAVAEFYELYHNNWISGMRKKLGIFNEEAE
                     DEALIEELLNIMHKNNEDYTNTFIKLTFGNTLNDSAEYKEWHNKWQARLQRQNKTKDK
                     LFELMKNSNPAVIPRNHRVEEALRTADENEDYSVMDKLISVLKNPFAHSADQKEYAEL
                     PPPSFCNYKTFCGT"
BASE COUNT          806 a          564 c          455 g          970 t
ORIGIN      
        1 taagcataat attatcaaga taacttctta tgattgctat ttctgctcta acataatatt
       61 ttttaatttc aatacattta ttattctgtt ttttgtttct atatctttgc atttaatagt
      121 gtggaggtgt ataccgtctg taagtctcaa gagaggctga actttctttt ctgcaatttt
      181 ttttaagaat aattctacgt catatcttga cctaatgtgc agttctccga taatttctcc
      241 atagataggg tgctctacaa taacatcttg cactgttcct ccgttatcaa caatagcaaa
      301 cagttcatcc cccagattct tttcatcatg gcagcatgcg actgtaaata cgcatttgct
      361 ttcttcttct ttgtcaaaaa cataacctct tggtgttgca gttattttga gaccgcctgc
      421 ccgcatgaga gcaatatctc ctactataac ctgacggctt acggaaaact ttttggcaaa
      481 atatgaagca gttacaggct ctgtgctatt ttttaatatt tcttcaattc gtgctcttct
      541 ttcctctgaa gtcatagtaa gcctccttta cgcagggatt accaatttag tcatatacaa
      601 atgccggcaa atattttgcc ggcaccttgt tgaaaaatcg gagtgaaagg atttgaacct
      661 tcggcctcat ggtcccgaac catgcgcgat accaagcttc gctacacccc gacaacacta
      721 acattataag ctctatgaaa atttttgtca atggaatttt cttgttgtcc acattcggtt
      781 cgttaaatct gcacatgaga ttaaccatca atatttaaaa cacatttaaa cttttgacat
      841 attataataa taaattatat gtacttttta ttttgtatta ttctaaattt tttggaggtt
      901 accaatgtat gacagatata tgccatattt ggcaaataat aaaatgcttg accttactta
      961 caagaaacta aaaagcctcg aatccccgca aatagggtat ttaaagctca atacttatcg
     1021 ccatgacaca agaagtccta tttccaatgc atttgtcaga gtatcaaaac ttaccataag
     1081 cggttattat agagaaatag gaacaggcgt ttttttggct gcaaattctt cagatgaaaa
     1141 tggttcggtt ccaatttttg aacttcccgt attaacgaat gaaaatgaaa tgtataatgt
     1201 atctgttaat tatctggatt actgccctgc ttatataatt gatattccga tatatccggg
     1261 tgtaacaacc acatatgatg tgtatatgca ccattattct atgaacggtg atgttgatat
     1321 ggactatcat ttattttgca gccgcaaata ccgggccgaa atacaggacc cgaaatattt
     1381 taaatctggt gtcattcttt ctatgtgccg caaaatgtct tataattgca aaatgacggc
     1441 ggcggaagct ctgcatattc tttttggtca gctgaatgag caaaaggatt ttttaacacg
     1501 cttattaatt tatccataac actgtaatcc tcattctcgt cagctgtcct tagagcttct
     1561 tctaccctgt gattgcgggg tatgacagca gggttggaat ttttcattaa ttcaaataat
     1621 ttatcttttg ttttattttg cctttggagg cgtgcctgcc acttattatg ccattcttta
     1681 tattctgcac tatcattcag tgtattgcca aaagttaact taataaaagt atttgtatag
     1741 tcttcattat ttttatgcat tatattaaga agttcctcaa taagagcttc atcttctgct
     1801 tcttcattga aaattcccaa tttttttctc atgcctgaaa tccaattgtt atgatataat
     1861 tcataaaact cggcaactgc atcctgggca agcttaattg ccttatcatt tttctcatgc
     1921 aaaagcggca gaagagtttc tgcaaatcta gataaattcc aaacagctat tttcggctga
     1981 tttttataag catatcttcc ataggtatca atagaagaaa aaacagtatc ggggttataa
     2041 gcatccatga aggcgcaggg accataatcg attgtttcac cgcttaatgc catattatcc
     2101 gtgttcataa caccgtgtat aaatccaacc agctgccatt ttgcaattaa tgatgcctgc
     2161 ctttttacaa cctctttcaa aaaactaaga tatggaaaat catccttttc aatttcctta
     2221 tagtgtctat caatggtata atcagccagt tgccttacat tttcttcggt tccgaaattt
     2281 gctgcatatt caaatgtacc gacacgaata tgactgcttg ctacgcgggt taaaattgcg
     2341 actttaagta ttttttctct tattacaggc tcaccggttg tcacaaccgc taaacttctg
     2401 cttgtaggaa tatccagagc atgcattgct tcgcttacaa tgtattcacg aagcatggga
     2461 ccaagcgcag ctcttccgtc tcctccccgc gaatatggag tcctgccgga gccttttaac
     2521 tgaatatcat atttcccatt cggtgtaatt tgttctccca agagcatggc tcttccgtct
     2581 ccaagcattg taaagtaacc gaattggtgt ccggcatagg cttgagcaat gggtatggag
     2641 ccttttggca cggagtttcc cgcaaaataa gatactcctt cctcactctg aagctcattt
     2701 atatccaaac ctaaatattc agctaattca ttatttaaaa caactaattt tggtgagctg
     2761 actttgctta aatttatttt ggaataaaaa aattc
//
LOCUS       DAOPUE010000180         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_37632_length_2782_cov_10.752109, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000180 DAOPUE010000000
VERSION     DAOPUE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37632_length_2782_cov_10.752109"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKH42_10035"
     CDS             <1..589
                     /locus_tag="PKH42_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOA20815.1"
                     /translation="NEFVRMVSVQNETISRYISDGRIIPDRIIETGYRNFNFFYEDTV
                     KSYAKNYNWDIITAANMMAKFMEMVEKMDMSYSYKPVLMYAMLENADEKGRVLIEDLI
                     DYFIYFYEDRRKKGLTVEKKKCIYLNGDYTRSDVKNNILSNPFKRFADMGFMRQSKDL
                     EYVEFNRNIWNKMTKEVTKQIIEYSDKALDRYYNG"
     gene            598..966
                     /locus_tag="PKH42_10040"
     CDS             598..966
                     /locus_tag="PKH42_10040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOA20816.1"
                     /translation="MSCIFCNIDKERIILENEGAFAVYDVFPVSQGHMLIMPKKHIKN
                     YFEVDEHTKTQLWKLVDECKEIVDKKFNPAGYNIGINCGEAAGQTVMHLRIHLIPRYL
                     GDIENPRGGVRGVIPNKRIY"
     gene            996..1829
                     /locus_tag="PKH42_10045"
     CDS             996..1829
                     /locus_tag="PKH42_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOA20817.1"
                     /translation="MEYKKSIINLIKERTSTRTYDGSGLEKEHLDDLNAYLDKVNQEI
                     KIKARFKIISNLGTGKKESKKLGTYGFIQGASTYIIGILDKDDNDALEFGYVFEKIVL
                     FATDLGVQTCWLGGTFSKDNFAELADLNENEFIAIISPVGYKKEKMRTFETTLRATIG
                     ADKRKPWNELFFNKSMKPLEEDEAGEFKMPLEMVRLGPSASNKQPWRIIKDEDMFHFF
                     ISRNKGYGITAYDLQKNDLGIAKCHFDLTLNELGIKGNWQELKDVLVPNDWEYAVSWT
                     L"
     gene            1881..2621
                     /locus_tag="PKH42_10050"
     CDS             1881..2621
                     /locus_tag="PKH42_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOA20818.1"
                     /translation="MKKIKNKIIVISIILVFILGAGFYNYLSDYYKAEPSAVELMNKD
                     QNISIVDDENMITFKPGNSSSDIGLVFYPGGKVEHLAYIPLMQKIAERGYTCVLMKMP
                     FNLAVFDIDAADRAFKEIAEVDFWYVGGHSLGGAMSSEFAQKNPEKIKGIILLGAYTS
                     GDLSNTDLKLLSIYGSEDKILNLEALEESKTKEPKDSRLQIIKGGNHAYFGNYKEQKG
                     DGIAYITNDEQQNMTADMIAEFFEASNE"
BASE COUNT         1041 a          327 c          593 g          821 t
ORIGIN      
        1 aaatgaattt gtccgcatgg tttctgtaca aaatgaaaca atatcaaggt atatttctga
       61 cggaagaata attcctgaca gaataataga aacaggttat agaaacttta actttttcta
      121 tgaagatacg gttaaaagtt atgccaaaaa ttataactgg gatataataa ctgcagcgaa
      181 tatgatggct aaatttatgg aaatggttga aaagatggat atgtcgtatt cttataaacc
      241 agtcttaatg tatgcaatgc tcgaaaatgc agatgaaaaa ggcagagtat taattgaaga
      301 tttaatagat tactttattt atttttatga ggacagaaga aaaaaaggat taacagtaga
      361 aaagaaaaag tgtatttatt taaatgggga ttataccaga agtgatgtaa aaaataacat
      421 tttaagcaat ccatttaagc ggtttgcaga catgggattt atgaggcaaa gcaaggacct
      481 agagtatgtt gaatttaaca gaaacatatg gaacaagatg acaaaagaag ttactaaaca
      541 aataatagaa tatagcgata aagcattaga cagatattat aacgggtaag gtgatgtatg
      601 agttgtatat tttgcaatat tgacaaagaa agaataattt tagaaaacga aggtgctttt
      661 gctgtttatg atgtttttcc ggtaagccaa ggtcatatgc ttattatgcc taaaaaacat
      721 atcaaaaact atttcgaggt tgatgagcat acaaagacac aattgtggaa attagtagat
      781 gaatgcaagg aaattgtgga taaaaaattt aaccctgcag gatacaacat aggaataaat
      841 tgcggagaag cagcaggtca aacagtgatg cacttgcgca ttcatttaat tccaaggtat
      901 ctgggtgaca ttgaaaaccc aagaggaggg gtgcggggag ttattcctaa taagcgcatt
      961 tattagggta tgtaaacaaa gaataggaga tcactatgga atataagaaa tcgataatta
     1021 atctaataaa agaaagaaca tctaccagga catatgacgg cagtgggctg gaaaaggagc
     1081 atttagacga cctgaatgca tatttggaca aagtaaacca ggagataaaa atcaaggcaa
     1141 ggtttaagat tatatccaat cttgggacag gaaaaaaaga atccaaaaag ttaggaactt
     1201 atggatttat tcagggagcc agcacttata ttattggtat tttggataag gatgacaatg
     1261 atgccttaga gtttgggtat gtatttgaaa aaatagtttt atttgccact gatttaggtg
     1321 ttcagacatg ctggctggga ggaacattca gcaaggataa ttttgcggaa ttggcagatt
     1381 tgaatgaaaa tgaatttata gctataatat cgcctgtagg atataaaaag gaaaaaatga
     1441 ggacttttga aacgacattg agagcaacaa tcggtgctga taaaagaaag ccctggaatg
     1501 agttgttttt taataagagt atgaagcctt tggaagaaga tgaagccggt gaatttaaaa
     1561 tgcctcttga aatggttaga cttggtcctt ctgcttcaaa taaacagccc tggagaatta
     1621 ttaaggatga ggatatgttt catttcttca ttagcagaaa taagggatat ggcataacag
     1681 cttatgactt acaaaaaaat gacctgggta ttgctaaatg tcattttgat ttaacactaa
     1741 atgaactagg aataaaaggc aattggcagg aactaaagga tgtcctagtt ccaaatgact
     1801 gggaatatgc agtttcatgg actttataat tattaaaaaa tatttcaata aacttgatat
     1861 agaaatagga atagagttat atgaaaaaaa taaaaaataa gataattgtt atatcaataa
     1921 ttttggtttt tatcctaggt gctgggtttt ataattattt atcagattat tataaagctg
     1981 aaccttcagc agtcgaattg atgaataaag atcaaaatat tagcattgtt gatgatgaaa
     2041 atatgattac atttaagcct gggaattctt cttctgatat tggtcttgta ttttatcctg
     2101 gcggaaaggt tgaacatttg gcgtatattc ctttaatgca aaagattgct gaaagaggtt
     2161 atacatgtgt tctgatgaag atgccgttta atcttgctgt tttcgatatt gacgcagccg
     2221 acagagcttt taaggaaatt gcggaagtag atttttggta tgtaggcgga cattctcttg
     2281 gtggggctat gtcatcagag tttgcccaaa aaaatcctga aaagataaaa ggtattattt
     2341 tattaggggc ttatacttcc ggagatttaa gcaatacaga tcttaaattg ctatctattt
     2401 atggttctga ggataaaata ttaaaccttg aagctttaga agagagtaaa acaaaagaac
     2461 caaaggattc aagacttcaa attattaaag gaggcaacca tgcttatttt ggcaattaca
     2521 aggagcaaaa aggcgatggt attgcttata ttacaaatga tgaacagcag aatatgactg
     2581 cagatatgat agctgagttt tttgaagcgt ctaatgaata aggataaaat gtctatgggc
     2641 gctatgatat gtctaataat gaaaatcaaa tgaatatgga gcatcggcag ctgatataat
     2701 ggtgtcatgg attagggatt aataacggta aatttatgca acatgaaggt aaggagctaa
     2761 cgcatgttaa atatgaagaa ag
//
LOCUS       DAOPUE010000181         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_38991_length_2715_cov_8.425188, whole genome shotgun sequence.
ACCESSION   DAOPUE010000181 DAOPUE010000000
VERSION     DAOPUE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38991_length_2715_cov_8.425188"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PKH42_10055"
     CDS             complement(<1..989)
                     /locus_tag="PKH42_10055"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOA20819.1"
                     /translation="MKLKVDKIVLLLICLSLIVGLYTIYQRVEIEKEYKTAEIVLDYN
                     EMKKFADSSNNDLSYWLRRFKEFGAESVAIQEETIKLLIEAGYSLRAEIVSELIKEYK
                     WQDGYSKKIVEAISENTIDPVDVIVTTEDEELFSYIVSGLEERYADDFFETYTFDNVY
                     YIVLNGIVDDIYYTDLEKVNNLAGKGVYEVSRVADSRLLNIGIGYDSKKISMAKEAGL
                     DVILRPINFPAYNEKLADAYKAANERYDLKPRIYITHGKEVLGYPDNEDRLLNYIKEE
                     NIAIALIESGTQREHLEQEGLNDLVEDSGYQALRAFTMWDFIRERNKYYNYEGA"
     gene            complement(1140..1976)
                     /locus_tag="PKH42_10060"
     CDS             complement(1140..1976)
                     /locus_tag="PKH42_10060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOA20820.1"
                     /translation="MVRLCMTVNKLFKLPVHPFNLHNEGKKTYAQWQYEKGIDTIKFY
                     LKHTDVDEMFKDKVVLDIGCGAGGKTVFYASKGVKKIIGIEILEKYRDEAESLATEYG
                     LEDKFEFVQGDASNMPFEDEIFDTIIMNDAMEHVDKPEKVLEECYRVLKKGGKLYLNF
                     PPYNHPYGAHLSDAIGIPWVHVFFSEKTLINTYKELVKNLPDGAERINFRISKKEDGT
                     EYFSYINKMSIKRANNILQNSNFKLEYYSEEPLRNFLKYPAKMPVLKEFLVKMVVCVL
                     RK"
     gene            2221..2700
                     /locus_tag="PKH42_10065"
     CDS             2221..2700
                     /locus_tag="PKH42_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20821.1"
                     /translation="MKKIIIFTLILAIALTAVIYAAPGDSNDPIVVLSYLNDRIKALI
                     SDYKLDEMENLQKKVDNLISSGGTGGSAALEVVEIYAGEKLIAGAGTELILRGGKAFV
                     IGSEKGGISNVTIGKDFVSGMEFVANHLMIVPRDDGRGAYTNEYAIFMVRGTYEITK"
BASE COUNT          813 a          546 c          430 g          926 t
ORIGIN      
        1 gcaccttcat aattgtagta tttatttctt tctcttataa aatcccacat tgtaaatgcg
       61 cgaagtgcct gataaccgga atcttcaacc aaatcattca aaccttcctg ctctaagtgt
      121 tccctttgag taccgctttc aatcaaggca attgcaatat tttcttcttt tatgtaattt
      181 aacagtctgt cttcattatc cggataacct aatacttcct tgccgtgtgt tatataaatt
      241 ctcggcttca aatcatatcg ttcattggca gctttataag catcagctaa tttctcgttg
      301 tatgcgggaa agttaatcgg tcttaaaatt acatccaaac cggcttcttt tgccatagat
      361 atttttttag agtcatagcc gataccgata tttaacaatc ttgaatcagc tacccttgaa
      421 acctcatata caccttttcc tgctaaatta tttacctttt ctaaatcggt gtagtatatg
      481 tcatctacga taccgtttaa aactatgtaa taaacattgt caaaagtata tgtttcgaaa
      541 aaatcatcag catacctttc ttccaaaccc gaaacaatat agctgaacag ttcttcatct
      601 tccgttgtaa ctataacatc tacggggtca attgtatttt ctgaaattgc ttctacaatt
      661 ttcttactgt aaccatcctg ccatttatac tctttaatta attctgaaac tatttcggct
      721 cttaagctgt aaccggcttc tatcaataat ttaatagtct cttcctgtat ggccacagac
      781 tcggccccaa attccttaaa tcttctcagc cagtagctta aatcattatt tgagctgtcg
      841 gcgaattttt tcatttcatt gtaatcaaga actatttccg cagttttata ttctttttca
      901 atttcgactc tctgatatat ggtatacaaa ccaactatca gcgatagaca aatcagaagc
      961 aatactattt tatcaacttt tagtttcatc ttatctcctt acaataaatt tatgacaagg
     1021 ggacgggggt agtatgacaa ggggacgggg gtagtgtcat ctaatgtcca ccgagaaccg
     1081 tccccgctgg tccaccgaga accgtccccg ccggtccacc gagaaccgtc cccgccggtt
     1141 cactttctca acacacaaac aaccatttta accaaaaatt cttttaatac aggcatctta
     1201 gccgggtatt tcaagaaatt tctaagaggt tcctccgaat aatattctaa tttaaaatta
     1261 ctgttttgta aaatattgtt tgcccttttt attgacattt tattgatata tgaaaaatac
     1321 tccgtgccgt cttctttttt gcttattcga aagttaattc tttcggctcc gtcgggcaaa
     1381 ttcttaacta attccttata tgtattaata agtgtttttt cgctgaaaaa cacatgcacc
     1441 catggtatac cgattgcatc gcttaagtga gcgccgtaag gatgattata tggagggaaa
     1501 ttcaaataca gcttcccgcc ctttttcaaa accctgtagc attcttcaag caccttttca
     1561 ggtttgtcga catgctccat ggcatcattc attattatag tatcaaatat ttcatcttca
     1621 aaaggcatgt ttgaagcatc gccttgtaca aattcaaatt tatcctctaa accgtattct
     1681 gtcgctaagc tttccgcttc atctctatac ttttccaata tttcaatacc tattattttt
     1741 tttactcctt ttgaagcata aaaaactgtt tttcctccgg ctccgcatcc aatatccaat
     1801 acaaccttat ccttgaacat ttcatcaaca tcagtgtgct taaggtaaaa cttaattgta
     1861 tctattcctt tttcatattg ccactgggcg taagtctttt tgccttcatt atgtaaatta
     1921 aaggggtgta ccggcagctt aaataatttg tttactgtca tacatagtct aaccataaat
     1981 ctaaccttct tacaaaaatt tgtcaaactg ttttctcctt tgcaattata tcatttcaca
     2041 ttggttaatt caatgtaaag ttcaacttat gtttattaag atatgaggac accacctatc
     2101 aagatacagg gacacctatc ggggaaaaag tgtaattctc taaaagattt gcattcttat
     2161 gttgaaattt atacttattt aatataaaat gtcatatata cttttaaggg agttggtaaa
     2221 ttgaaaaaga taattatatt tactttaatt ttggcaatag cactcactgc agtaatttat
     2281 gccgctccgg gtgacagcaa tgacccaata gttgtactaa gctacttaaa tgaccgcata
     2341 aaagcattaa tcagcgatta taaattagac gaaatggaaa acctgcaaaa aaaagttgat
     2401 aacttgattt caagcggcgg tacaggcggt tcggctgcct tggaggtagt tgaaatatat
     2461 gcaggcgaaa aactgattgc aggagcaggt actgagctta ttttaagagg cggcaaagct
     2521 tttgtaatag gaagtgaaaa gggtggaatt tcaaacgtta caatcggtaa ggatttcgtt
     2581 tcggggatgg agtttgttgc caatcattta atgatagttc caagagatga cggtcgaggt
     2641 gcctatacga atgaatatgc catatttatg gttaggggta catacgaaat aactaagtaa
     2701 aacaagggac acaag
//
LOCUS       DAOPUE010000182         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_39127_length_2709_cov_8.792012, whole genome shotgun sequence.
ACCESSION   DAOPUE010000182 DAOPUE010000000
VERSION     DAOPUE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39127_length_2709_cov_8.792012"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PKH42_10070"
     CDS             complement(<1..710)
                     /locus_tag="PKH42_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOA20822.1"
                     /translation="MEQDNKRGQWASNFGFLMAAIGSAIGLGNIWGFPYKMGANGGFI
                     FLLIYLTLVAFVGFATMLGELTLGRKYGMGVIGTYKKISEKYAWIGWLGWLSGFFILA
                     FYSVLGGFTVRYFFGFLMEMFSAGAGFGGMDSGAFFGSFILSAPMSILFHAIFMGATV
                     IIVMGGISGGIEKFTAVAMPTLFVMVIIVVIRSITLEGAFVGLEFMFKPDFEALKEIG
                     FMRVLKTAAGQMFFSLSLG"
     gene            complement(1120..2469)
                     /locus_tag="PKH42_10075"
     CDS             complement(1120..2469)
                     /locus_tag="PKH42_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOA20823.1"
                     /translation="MSTKNEVFGYLPDERPPIIGLIFFALQQIVVMFPATVLVALITG
                     FHVSTTIFASGLATLGFILITGRQIPLYYGSSFSYIAAIASIMSAEQFALYSLNDKIS
                     VAQFGIVMSGFVSILAGYIINKSGKDKIDKVLPATVTGSIAIIIGLSLSANAMTNAAA
                     FPEVAQSNPYASSWAWVIAIITLLSTILYSVYLRGKLSQLPILFGLMTGYVAALVIGG
                     VSGIPFVSFDTIQSTGVFNLPIFTFPKPTLTAVVAIMPIAIATIPESTAHVYQLDIYV
                     NDLAKKKGAGKIYDIENKLGLNLIGDGMGDIISGFIGGPAGTNYGENISAMAITKNFS
                     VHVLILASILTMIISCFTPLINIIYSIPTAVIGGLSIYLFGVIGAQGITIMLDRKVDM
                     FSAKNLAVIAVILIVGLGGSFGFAGGMIPMFGIEVPAIASAAVVGILLNLMLSIGEK"
BASE COUNT          918 a          543 c          402 g          846 t
ORIGIN      
        1 ccgagagaca gtgagaagaa catctgtccg gctgcggttt ttagaaccct cataaaacct
       61 atttccttca atgcctcaaa gtcaggttta aacataaatt caagacctac aaaagcacct
      121 tccaaagtaa tacttctgat aactacgatt ataaccatta caaacaaggt tggcatagcc
      181 accgccgtaa acttttctat accgcctgaa attccgccca ttactataat tactgtagcg
      241 cccataaaaa ttgcatgaaa taatatactc attggagcac ttagtatgaa gctgccaaag
      301 aatgcaccgc tgtccattcc tccaaaaccg gcacctgcgc tgaacatttc cattaagaat
      361 ccaaagaaat aacgtacagt aaaaccgcct aatacgctat aaaatgctaa aatgaaaaat
      421 cctgaaagcc agcccagcca tcctatccat gcgtactttt cgctgatttt cttataagtt
      481 ccgataactc ccataccata tttacggcct aatgtaagct ccccaagcat agttgcaaag
      541 cctacaaaag ctaccaaagt caaataaatt aatagaaata taaatccacc gtttgcaccc
      601 attttatatg ggaatcccca aatattaccc aagcctatag ctgagcctat agctgccatc
      661 aagaaaccaa aattactggc ccactgacct ctcttattat cttgttccat tttacaccat
      721 cctttcttct tttgagatac gaatgcaata ccaataatta cttattatta actatatcta
      781 aataacctcc tatctttttt aagttttttt taatttcacc cttataaaat aaggttttat
      841 aacgtatgca tttaataatt ttatgtataa aaatgaatgc ataaaatact attatgtccc
      901 tgcaaacgta tcctttcatg aattatacta tatagcattt ggtttgtcca gcaaaaacta
      961 tgttatattt aaaacaatca atgaataaaa taataattca taatgtttat attattcatt
     1021 tgtataaaat actgcatatg aatatatatt gatatacact aaataaagat tcctctacgg
     1081 tcagaggaat cttatttttt gtatattatt ttttgtatac tatttttcac ctattgacag
     1141 cataagatta agaagtattc cgacgacggc tgctgaagct atggcaggta cttcaatacc
     1201 aaacatagga atcataccgc ctgcaaatcc aaagctgccg ccaagcccta ctattaaaat
     1261 tacagctatc accgctaaat tttttgcgct gaacatatca acctttctat ccagcataat
     1321 ggttataccc tgtgctccta taacaccgaa taaataaatc gataatcctc cgattactgc
     1381 tgtgggaatt gaatatatta tattaattaa aggtgtaaag caggaaataa tcattgttaa
     1441 tattgatgct aatataagaa catgtacgga gaaatttttg gttattgcca ttgcacttat
     1501 attttctcca tagtttgtac ctgcaggacc tccaataaat cctgaaataa tgtcccccat
     1561 tccatcgcct ataagattta atcctaattt attttcaatg tcatatattt tgccggcgcc
     1621 ttttttctta gctaaatcat ttacatatat atctaactga taaacatgtg ccgtagattc
     1681 cggtattgtt gctattgcaa tgggcataat tgccactact gcagtaaggg taggtttagg
     1741 gaatgtaaat atgggaagat taaacactcc tgttgattga atagtgtcaa atgatacaaa
     1801 gggtatacct gatactcctc ctattactaa ggcggcaaca tatcctgtca ttaacccgaa
     1861 caaaattggc agctggctta gttttcctct taaatacacc gaatataaaa ttgttgataa
     1921 taaggtaatt attgctataa cccatgccca actgcttgca taaggattgc tttgtgcaac
     1981 ttcaggaaag gctgctgcat ttgtcattgc attggctgac aaagacaaac ctataattat
     2041 tgcaatactt cccgttactg ttgctggcag aactttgtct attttgtctt tgccggattt
     2101 attaataata tatcccgcaa gtattgaaac aaaacctgac attacaattc cgaactgagc
     2161 aactgaaatt ttatcattta gggagtataa agcgaactgc tcggcactca taattgaagc
     2221 tattgcagca atatatgaaa agcttgagcc atagtagaga ggaatttgtc ttccagtgat
     2281 aagtataaat cctaatgttg ccaaaccgct ggcaaaaatc gtagtagata catgaaaacc
     2341 tgtgattaga gcaaccaaga ctgttgcagg aaacattacc actatttgtt gtaatgcaaa
     2401 gaaaataagt ccaataatcg gcggacgctc gtccggcaaa taaccaaaaa cctcattttt
     2461 tgttgacata aatacctccg tgtaataaat aaaatcctcg accggatggt caaggattag
     2521 tatacacaat aactcatatg aatattaaat cttgacggca tcacggtacc gtgttaaaga
     2581 ttattttcac caattataac atgtgtaaat aactcagtca acaagaaaat ttttactgat
     2641 tgattttttg attgttacgt gaattgacaa agtaaatgca accctatgat tcaaatatag
     2701 ttgcattta
//
LOCUS       DAOPUE010000183         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_40049_length_2664_cov_9.821388, whole genome shotgun sequence.
ACCESSION   DAOPUE010000183 DAOPUE010000000
VERSION     DAOPUE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40049_length_2664_cov_9.821388"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /gene="rpsG"
                     /locus_tag="PKH42_10080"
     CDS             <1..284
                     /gene="rpsG"
                     /locus_tag="PKH42_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOA20824.1"
                     /translation="KAMNNIMPVLEVKARRVGGATYQVPIEVRPERRQTLGLRWLVTY
                     SRKRGEKTMRERLAKELMDAANNVGASVKKREDTHKMAEANKAFAHFRW"
     gene            325..2385
                     /gene="fusA"
                     /locus_tag="PKH42_10085"
     CDS             325..2385
                     /gene="fusA"
                     /locus_tag="PKH42_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA20825.1"
                     /translation="MREYSLENTRNIGIMAHIDAGKTTCTERILFYTGKIHKVGETHE
                     GAAQMDWMEQEQERGITITSAATTCIWKDVRVNIIDTPGHVDFTVEVERSLRVLDGAV
                     AIFDAKMGVEPQSETVWRQADKYHVPRICFVNKMDKMGADFFYSVGTMIERLKANAVP
                     IQLPIGAEDKFNTIIDLVTMKATKYNNDEGTALEVIDIPEDYLDKAQEYREKLIEALS
                     DFNEEIMEKYLNGEEISIAELKKAIRACTIKNEIVPVLCGTAYKHKGVRLMLDAVVDY
                     LPNPLDVPQLSGFDPHTNEVIVRDASDDAPLSALAFKIATDPFVGKLTFFRVYSGVLQ
                     SGSYVLNSTKNKKERIGRILQMHANKREEIDNSYTGDIDAAVGLKITTTGDTLCDPDH
                     PIVLESMVFPEPVIEVAIEPKTKAGQEKMGIGLQKLAEEDPTFKTYTNPDTGQTIIAG
                     MGELHLEIIVDRLLREFKVEANVGKPQVSYKETVTGKADVDVKYARQSGGKGQYGHVK
                     ITMEPQEPGQGITFVNAIVGGVIPKEYIPAVEAGIKGAAENGILAGYPVIDFKVTLYD
                     GSYHEVDSSEMAFKIAGSMAFKQAMEKASPVLLEPYMKVEITTPEEYLGDVMGDVNSR
                     RGRIEGFSDRLGIKVVDAYVPLSEMFGYATTLRSLTQGRASYSMEFHHYEKVPNNIAE
                     KIVK"
     gene            2432..>2664
                     /locus_tag="PKH42_10090"
     CDS             2432..>2664
                     /locus_tag="PKH42_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOA20826.1"
                     /translation="MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITLILNKRFGTGE
                     FVSYDNIDKAPEERERGITISTSHVEYETQNRH"
BASE COUNT          974 a          429 c          607 g          654 t
ORIGIN      
        1 ataaagcaat gaacaacatc atgcctgtgt tggaagttaa agcaagaaga gtcggtggtg
       61 ctacttatca ggtacctatt gaagtaagac ctgaaagaag acagacctta ggtttaagat
      121 ggcttgtaac ttattctcgc aaaagaggag aaaaaactat gcgcgaaagg cttgcaaaag
      181 aattaatgga tgcagccaat aacgttggag ccagtgttaa gaaaagagaa gatactcata
      241 aaatggcaga agctaataag gcatttgcac attttagatg gtaattaagg tagtacaaaa
      301 aaagcagcgt gaaggaggga agcaatgaga gagtattctt tagaaaatac cagaaatatt
      361 gggataatgg cgcatattga tgcgggaaaa actacatgca ccgaaagaat cctgttctat
      421 accggtaaga ttcataaagt aggcgaaact catgaaggtg cagcacaaat ggactggatg
      481 gagcaagagc aggaaagagg aattacaata acttcagctg cgactacttg tatatggaaa
      541 gatgtaagag tcaatattat tgatacaccg ggacacgtag acttcacggt tgaagttgaa
      601 aggtccttga gagtattaga tggtgctgta gcaatatttg acgcaaagat gggcgttgag
      661 ccgcagtcgg aaactgtctg gagacaggca gacaaatatc atgtaccaag aatttgtttt
      721 gtaaacaaaa tggataaaat gggagcagat ttcttttatt cggtcggtac catgatagaa
      781 agattaaaag caaatgctgt tcctattcag cttcctatag gggctgagga taagttcaac
      841 actattatag accttgttac tatgaaagca acaaaatata ataatgatga agggacagcc
      901 ctggaagtta tcgatatacc tgaggattat ttggataagg cacaggaata tagagagaaa
      961 cttatagaag ctttatcgga ttttaatgaa gaaataatgg aaaaatattt aaacggcgaa
     1021 gaaatttcaa ttgcagagtt aaaaaaagca ataagagcat gcactattaa aaatgaaatt
     1081 gtaccggtat tatgcggtac agcttacaaa cataaaggcg taagacttat gctggatgca
     1141 gttgttgatt atttgcctaa tccgttagat gttcctcagt tgagcggatt tgatcctcac
     1201 actaatgaag taatagttag agatgcgtca gatgatgctc cattatcagc tttggcattt
     1261 aaaatagcaa cagacccctt tgtagggaaa cttacattct tcagggttta ttcaggggta
     1321 ttgcaatcag gctcttatgt cttgaattca accaagaaca aaaaagaaag aataggcaga
     1381 atacttcaaa tgcatgccaa taagagggaa gagattgaca actcttatac gggagatatt
     1441 gatgctgccg ttggtctaaa aatcacaaca acaggagata ccttgtgtga ccctgaccac
     1501 cccattgttc tggaatccat ggtattcccg gaacctgtta tagaggtggc aatagagcca
     1561 aaaactaagg caggacagga gaaaatgggt atcggacttc aaaagttagc tgaagaagat
     1621 cctactttta aaacatatac aaatccggat acaggacaga caattattgc aggaatgggc
     1681 gagctgcatt tggagataat agtcgacaga ctgttaagag aattcaaggt tgaagcgaat
     1741 gtaggcaagc cgcaagtttc ttacaaagaa acagtaacag gcaaagcaga cgttgacgtt
     1801 aagtatgcaa gacaatccgg aggcaaggga caatatggtc atgttaaaat taccatggag
     1861 cctcaagaac ccggtcaagg cattactttt gtcaatgcta tcgtaggagg agttattcct
     1921 aaagaatata taccggcagt tgaagccgga attaagggcg ctgcagaaaa tggtatttta
     1981 gccggttatc cggtaataga ctttaaagtt acactgtatg atggttcata ccatgaagtt
     2041 gactcgagcg aaatggcatt taaaattgcc ggctcaatgg cgtttaaaca agcaatggaa
     2101 aaagcatcgc cggtgctctt agagccttat atgaaggttg aaataacaac tcccgaagaa
     2161 tacttgggag acgttatggg agacgtaaac tcaagaagag gaagaataga aggtttctcg
     2221 gacagactcg gtattaaggt tgttgatgct tatgttccgt tatcggaaat gtttggatat
     2281 gcaacgacac ttcgctcgct gactcaagga agagcttctt attcaatgga attccatcat
     2341 tacgaaaaag tgcccaacaa tatagctgaa aaaattgtaa aataaaacaa aatataacat
     2401 tataaataaa cagcctaagg gaggaaaaga aatggcaaaa gcaaaattcg aaagaaataa
     2461 accccatgtt aatataggaa caattggtca cgttgaccac ggcaaaacta cattaacagc
     2521 agcaattaca cttattttga acaaaagatt cggaacggga gaattcgtaa gctatgacaa
     2581 catagacaaa gctcctgaag aaagagaaag aggaattaca atttcaactt ctcacgttga
     2641 atatgaaact caaaacagac acta
//
LOCUS       DAOPUE010000184         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_41072_length_2616_cov_11.421710, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000184 DAOPUE010000000
VERSION     DAOPUE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41072_length_2616_cov_11.421710"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1635
                     /locus_tag="PKH42_10095"
     CDS             319..1635
                     /locus_tag="PKH42_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOA20827.1"
                     /translation="MNKPVLSAHFESRVPSDVRLSQMKFAERKNKPEAVINVGIGNVS
                     LPTNPAMQKRMFNLDAPESPFNKGVIRYTATNGFAETQDAFKHILKCQGFDTSKLSVV
                     VTDGGSAGMELLIMGVCGPAATDEKPLMMIDPSYSNYISFAERVGRKTVTVKRHLNED
                     GKFSLPEIDKIEETIKAYKPGALLVIPYDNPTGQFYDKETLISLAELCVKYNMWMASD
                     EAYRELFYVEGSDLVSIWGITDKDVPGIEGRRISIETASKVWNACGLRIGAVITDSPE
                     FGNRCAAEYTANLCANAIGQYIFGALAHETKEQIAAWCKDIRNYYGDMIKNVTAEFKK
                     LEPDLIVSSPDASIYTVVDVRNVVKPGFDSIEFCLYTAGEGAVDLDGVQTTLLVAPMK
                     GFYDIKKGEHNPGSTQFRLSYVETPENMMKVPKLFVELLKQYEASR"
     gene            complement(1677..2558)
                     /locus_tag="PKH42_10100"
     CDS             complement(1677..2558)
                     /locus_tag="PKH42_10100"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOA20828.1"
                     /translation="MINKTISLILALMLMATPHVKCPEVIKILSIGNSFSQDCVYYLY
                     DIAQSAGVNVIIGNLYHSGSSLERHDKNAKENLKAYSYQKWTSSEMVEEKNKTMKEVL
                     LDEDWDYITFQQSSENSGLYDTYQPYLNNLINYVKSISKNPDVKFALNMTWAYSTRSS
                     NKGFAYYNYSQFNMYRCITESYKRAIEETEIDIIIPCGTAIQNARTNEHLNSIGNQLT
                     NDGYHLENGIGKYIAGLTFFETLVINDKNLDLNIYDDVTFIPETKNERADLADIAKKS
                     VIKAVNYPYIITKIRAD"
BASE COUNT          895 a          414 c          486 g          821 t
ORIGIN      
        1 cattgattag attaggacac aagtatgaag taggaatagg tttaaaaaca ttgctcgaaa
       61 aaatgtaatt aatatataaa gcagctatcg attagctgct ttgtttttgc aaataataaa
      121 aaaatactct ttaccgatta cagttcacac ataatagcag taaatgaata tttggcgaaa
      181 cagtaacaaa caataaatat taaataccat ataattaagc taaatgtatg gacaaaaaac
      241 ttaaagttcg ttataattat atttaatagg tactattaaa tttatatata acaatatatt
      301 aataggaggg ttttataaat gaataaacca gtgttgtcag cacattttga atcaagagta
      361 ccttcagatg ttagactgtc acaaatgaag tttgcagagc gtaagaataa acctgaagct
      421 gtaatcaatg taggaatcgg taatgtttcg ttgcctacaa atccggctat gcaaaaaaga
      481 atgtttaact tagatgctcc tgaaagcccg tttaataagg gtgtaatcag atacactgcc
      541 accaacggat ttgcagagac acaggatgca tttaaacaca ttttaaaatg tcaaggtttt
      601 gacacaagca agctgagcgt tgtagttaca gacggcggtt cagccggtat ggaattgcta
      661 ataatgggag tttgcggacc tgcggcaaca gatgaaaaac ctttgatgat gattgaccca
      721 tcatacagta attatatttc ttttgctgaa agagtaggac gtaagactgt tacagttaaa
      781 agacatttaa atgaagatgg aaaattcagc cttccggaaa ttgacaaaat agaagaaaca
      841 ataaaagctt acaagccggg agcacttctt gttatacctt atgacaaccc tacaggccag
      901 ttttatgaca aagaaacatt aatttcactt gcagagctat gcgttaaata caatatgtgg
      961 atggcaagtg atgaagctta tcgtgaatta ttctatgttg aaggaagcga tttagtaagt
     1021 atctggggaa ttacagataa agatgtgccg ggtattgaag gaagaagaat aagcattgaa
     1081 actgcatcaa aggtatggaa tgcatgcgga ttaagaatag gagccgttat taccgacagc
     1141 cccgaattcg gtaatcgctg cgcggcagaa tatacggcaa acctatgtgc aaatgcaatc
     1201 ggacagtata tatttggagc attggctcat gaaacaaaag aacaaatagc agcttggtgt
     1261 aaagacattc gtaattacta tggcgatatg ataaaaaatg taacggcaga gtttaaaaaa
     1321 ttagagcccg atttaattgt ttcaagccct gatgcatcaa tttacactgt agtggatgtc
     1381 aggaatgttg taaaaccggg atttgattca attgaattct gcctgtatac tgccggagaa
     1441 ggtgctgtag acttagacgg agttcaaaca acactattgg ttgctcctat gaagggattc
     1501 tatgatatta aaaagggtga acacaaccct ggaagtactc aattccgctt gtcatatgtt
     1561 gaaactccgg aaaatatgat gaaagttcca aagttatttg tagagctttt aaaacaatac
     1621 gaagcaagca gataatatga caatgtgata taccttaatc agtaagagct gacttattag
     1681 tcagccctta tttttgttat tatatatgga taatttactg cttttattac ggactttttc
     1741 gctatgtcag ctaaatcggc tctttcattt tttgtttcag gtataaatgt cacatcatca
     1801 taaatattca agtctaaatt cttatcatta ataaccaggg tttcaaagaa agttaatcct
     1861 gcaatatatt ttcctatacc attttctaaa tgatagccgt cattggtgag ctggttacca
     1921 attgaattga ggtgttcgtt tgtccgagca ttttgtattg ccgtaccgca tggaattatt
     1981 atatcaattt ctgtttcctc aattgctcgt ttgtaggatt ctgtaatgca gcggtacata
     2041 ttgaattggc tatagttata ataggcaaat cctttattgc tgcttcttgt tgaataagcc
     2101 caagtcatat ttagagcaaa tttaacatca ggattttttg aaattgattt aacataatta
     2161 atcaaattat tcaagtaagg ctgataagta tcgtataatc ccgaattttc cgaagattgt
     2221 tgaaatgtta tgtaatccca gtcttcatct agcagcactt ctttcattgt tttgtttttt
     2281 tcttctacca tttctgaaga tgtccacttt tgataggaat acgccttaag attttcctta
     2341 gcattcttat cgtgtctttc caaagagctg ccgctgtggt acaagttacc gattattaca
     2401 ttaacacctg cggactgtgc tatatcatac aaataataaa cacaatcttg tgaaaaagaa
     2461 ttgccgatag acagaatttt tattacttcg ggacatttta catgaggagt tgccattaac
     2521 attagagcta atattaaaga tatcgtctta ttaatcatta tgtcaccacc aaaattagta
     2581 taatacaaaa ttagacaaaa tccaactcta aaaatg
//
LOCUS       DAOPUE010000185         2560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_42486_length_2560_cov_10.577246, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000185 DAOPUE010000000
VERSION     DAOPUE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42486_length_2560_cov_10.577246"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..686
                     /gene="greA"
                     /locus_tag="PKH42_10105"
     CDS             216..686
                     /gene="greA"
                     /locus_tag="PKH42_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOA20829.1"
                     /translation="MKGNETLITIEGLEELKRELEELKIVKRKEISEKIKAALAFGDI
                     SENAEYDEAKNEQAIVEARIAKLETMIKNAKVIKTTKKNGVVCIGSKVRIKDLEFDEE
                     MEYTIVGSAEADPFKGRISNVSPLGKALLGKKLGDIIEVASPAGDMIKYEILTV"
     gene            719..2191
                     /gene="lysS"
                     /locus_tag="PKH42_10110"
     CDS             719..2191
                     /gene="lysS"
                     /locus_tag="PKH42_10110"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009526720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOA20830.1"
                     /translation="MDEQQISDLRQIRISKLKDLINAGRDPYKVSKYEVTSNSKQIKD
                     NFEEMEGKFVSIAGRIMSKRGQGKVGFYDIQDHLGRIQMFVKLDNIGEEEYNWLKTYD
                     IGDIIGVKGEVFKTKTGEVSVRAEEIKLLSKSLQTLPEKFHGLKDMDLRYRQRYVDLI
                     VNPEIKEVFLMRNKIIRGIREYLDGEGYLEVETPILSPIAGGANARPFITHHNTLDID
                     MYMRIANELFLKRLVVGGLGDGVYEMGKMFRNEGMDANHNPEYTAMEVYKPYADFNDM
                     MTLTENIVAYVAKKARGTTKIEYQGKMIDFTPPWRRVKMQDMVKEYAGVDFDLINTDK
                     EAIEIAKEKGIEITPLMTRGHVISELFEKYCEEHLEQPTFVTHHPVEISPLAKRNAED
                     PRLTDRFEAFANTWEIANAFSELNDPIDQRERFEAQVRQKEAGDEEAHMMDTDFINAI
                     EVGLPPTGGLGIGVDRVIMLITNQPTIRDIILFPTMKPLN"
BASE COUNT         1037 a          342 c          518 g          663 t
ORIGIN      
        1 aattttggat aagtacattg aagtttattg cttaaatgac cttaacggtt taaattaaca
       61 aattaacttg acaataatcg tttacttgtt tataatgttc taattaattg aaagatttgt
      121 catcaaatta tcataatata ttaaaatgat aataaaatga aatatatatt gatgacatgt
      181 aaatataagg taacattatt aaaaggagca atattatgaa aggtaatgaa actttgatta
      241 caattgaggg tttggaagaa ttaaaaaggg aattagaaga attaaaaata gtaaaaagaa
      301 aagaaatatc cgaaaaaatt aaggctgctt tagctttcgg ggatataagc gaaaatgccg
      361 aatatgacga agccaagaat gaacaagcaa tcgttgaagc gagaattgca aagcttgaga
      421 caatgataaa aaatgcgaag gtgataaaaa caacaaagaa aaacggagtt gtatgcatag
      481 gctcaaaagt tagaataaag gatttggaat ttgacgagga aatggaatat acaatcgtag
      541 gttccgctga agcagatccg tttaaaggaa gaatatctaa tgtttcacct ttgggaaaag
      601 ctttattggg aaagaaactc ggtgatataa tagaagttgc atcccctgcc ggagatatga
      661 taaaatatga aatactaacc gtttaaataa aacaactaat gatgaggtgg tgttaaaatt
      721 ggatgagcag caaataagcg acttaagaca aataaggatt agcaaattaa aagatttaat
      781 taatgcagga agagacccct acaaagtttc aaaatacgaa gtaacctcta attcgaaaca
      841 aatcaaggat aattttgaag aaatggaagg aaaattcgta tcaattgccg gaagaataat
      901 gtctaagaga ggacagggaa aggttggatt ctacgatata caggaccact tgggaagaat
      961 ccagatgttt gttaaactgg acaacatagg cgaggaagaa tacaattggc tgaaaactta
     1021 cgatattggc gatataatag gcgttaaggg tgaagtattc aaaacgaaaa caggagaagt
     1081 ttccgtaaga gccgaagaaa taaaactttt atccaaatcc ttgcaaacac ttcctgaaaa
     1141 gttccacgga ttaaaggata tggatttaag atatagacaa agatatgtgg atttaattgt
     1201 taaccccgaa atcaaagaag tttttttaat gcgtaataaa ataataagag ggattagaga
     1261 atatttagac ggagaaggat atctagaggt tgaaactcct attttaagtc caatagcagg
     1321 cggagcaaat gcaagaccat tcattactca tcataataca ttggatattg acatgtatat
     1381 gcgcattgct aatgagttat ttttaaagag acttgtggtt ggagggcttg gagacggcgt
     1441 ttacgaaatg ggaaaaatgt ttagaaatga aggaatggat gcaaaccaca accctgagta
     1501 taccgccatg gaagtatata aaccatatgc agacttcaat gatatgatga ctttaacaga
     1561 aaatatagtt gcatatgttg caaagaaagc aagaggtaca actaaaatag aataccaggg
     1621 caaaatgata gactttactc cgccttggag aagagttaaa atgcaggata tggttaagga
     1681 atatgcagga gttgatttcg acctgattaa taccgataaa gaagcaattg aaattgcaaa
     1741 agagaaagga attgaaatca cgcctcttat gacaaggggt catgtaataa gcgaattgtt
     1801 tgaaaagtat tgtgaagagc atttggaaca gccaaccttt gtaacacatc atcctgttga
     1861 aatatcacct ttggctaaga gaaatgcaga agacccgcga ttaacagata gatttgaagc
     1921 atttgcaaac acatgggaaa ttgcaaacgc attttcggaa ttaaatgacc ctattgacca
     1981 aagagaaaga tttgaagcac aagttaggca aaaagaagcc ggtgatgaag aagcccatat
     2041 gatggataca gacttcatta atgcaattga agtaggtctc cccccaacag gcggactcgg
     2101 cataggagtt gacagagtaa ttatgttaat aacaaaccag cccacaataa gagacatcat
     2161 attgttccca acgatgaaac cattgaatta attataaaaa aatggcaata attgaaccga
     2221 cctcctaagg tttacactga gatctaactg gtggaggtct ttttttattt agttatataa
     2281 ttatgatgga agtaaataaa agcatattaa aacggataag ataaaaacaa atattttgct
     2341 aaaaattatg gttgacaatt aataaaataa atgctagact gtacaagttg tccaatgtgg
     2401 ataaattgta aaaaacaggc aaatatcaag taaattgtaa gaaaaaaaca gcttgacaac
     2461 atatttcgcc tatgatagac tgtaaaagct cctctaaaaa ggagcatcga acatagacaa
     2521 taaaacagca taagaaacct ttgagtacgc aatgaaaatc
//
LOCUS       DAOPUE010000186         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_44107_length_2493_cov_13.297375, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000186 DAOPUE010000000
VERSION     DAOPUE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44107_length_2493_cov_13.297375"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..1651
                     /locus_tag="PKH42_10115"
     CDS             236..1651
                     /locus_tag="PKH42_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HOA20831.1"
                     /translation="MIKVKRNIPFIDLLNERNRAVLVHSLFSSWMLSLIFEGQIFQYL
                     AKEYNFEVGSIITASSAAMFLGLLAGGFIIKTKLHAKRLFLFSYPFFAALSLIFFFSP
                     SLLWAIGIIAGSLISGCCVAAWGFYFKTVSKKNERLKTAADVLIISNILMIILNSATI
                     NISTYVGLSLSIIILIFAFMIALKLPSDFNESYRYKKEESLSISTLLLFFYLFIVILT
                     INSGLMYHVVNPAFEHLTALISWYWAVPYVGAILILRNFSGKIKSSYILYVAIAMIGL
                     SFVAFITLDRSATSYFIINTLMLGSYGVYDLFFWSILGEMLNYHENPAKILGIGLSAN
                     VFGVALGGLIGNNLIANNLRSLSPSLLAFAIVCITLVLLPPLNKHLSSLLKGQTFLTE
                     FIEMPESKQKSEIDKLSTFGNLSEREKQVASLLLQGKTYKTIAAELFISENTVKYYVK
                     NIYSKFNIQSRAELIDIIVEK"
     gene            1850..2428
                     /locus_tag="PKH42_10120"
     CDS             1850..2428
                     /locus_tag="PKH42_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20832.1"
                     /translation="MNAIRKRIIIYISIICFLIAAWAQLFSLQGGNFNAQTCVITIIT
                     VAGGIIPIYLLFKDINNLKIARLIKENFILKVKTAVISEISEKEIPYKPINIEDTEIF
                     VSYFGILFNGKIAKFNQDGIRLTAMDIGNDYISFTYGNEKQMKNIRIVRPDIEPDAMS
                     EMINKFRFEMGITPTMVGKIITNKCNNEVKYG"
BASE COUNT          824 a          388 c          441 g          840 t
ORIGIN      
        1 gcatcaaatt ccttctggta gttactcgta accatataat cactcctctc cttatcgtgg
       61 ttcaaccacc aaccacagta taatatatgc agggaaaagt gtcaatatga aaaagcagat
      121 tgttatttgt attataattt agtataatgt atattatcat gcagtttaaa ctttagttta
      181 tgaatatgaa tataattaag tataagcaaa tgattcatgg agggcgggct aaatcatgat
      241 taaggttaaa cgaaatattc cctttattga tttgttaaat gagcgcaata gggcggtatt
      301 ggttcattca ttgttttcct catggatgtt gtcattaata tttgaagggc aaatatttca
      361 atatcttgcc aaagagtaca attttgaagt gggatcaata attaccgcta gttctgccgc
      421 tatgttttta ggattactgg ccggcggatt tattataaaa acaaaactcc atgcaaaacg
      481 gcttttcctt ttttcctatc cattttttgc tgctttatca ttaatatttt tcttttcacc
      541 ctcattactg tgggctatcg gaataattgc cggctccttg atttcgggat gctgtgttgc
      601 agcatgggga ttttatttta aaactgtttc aaagaagaac gaacgtttaa aaacagcagc
      661 agatgttctc ataatttcaa acattttaat gataatacta aattcagcca ctataaatat
      721 atccacatat gtcggactgt cattgtccat tattatactg atatttgcat ttatgattgc
      781 tctgaaactt ccttcggatt ttaatgaaag ctacaggtat aagaaagagg aaagtctaag
      841 tatttcaacc ctcttattat ttttttatct gtttattgta atattgacaa ttaattcagg
      901 cttgatgtac catgtggtaa atccggcttt tgagcatctt acagctctca ttagctggta
      961 ctgggcagtt ccttatgttg gggcaatttt gattctaaga aatttttcag ggaaaataaa
     1021 atcttcatat atactatatg ttgcaattgc aatgattggt ctttcgtttg tagcttttat
     1081 aacacttgac cggtctgcaa caagctattt tataattaat acgttgatgc ttggctccta
     1141 tggtgtctat gacttgtttt tctggagcat attaggtgag atgcttaatt accatgaaaa
     1201 ccctgcaaaa attctgggca taggtctttc tgccaatgta tttggagtag cattaggggg
     1261 acttatcggt aacaatctaa ttgcaaataa tctacgaagt ttaagtccat ccctgcttgc
     1321 ttttgctata gtttgtatta cattggtgct tttacctcct ctaaataagc acctttcttc
     1381 attgcttaaa ggacaaacct ttcttactga atttattgaa atgcctgaat ccaaacaaaa
     1441 aagtgaaata gataagcttt caacattcgg caatttgtcg gaaagagaaa aacaggtggc
     1501 gtcacttctt ttgcagggta aaacatataa gacaattgct gctgagcttt tcatcagcga
     1561 aaacacagta aaatattacg taaagaatat ttattctaaa tttaatattc aaagccgagc
     1621 agagctcatt gatataatag tagaaaaatg aaaaataacc acctacccga aaagggtagg
     1681 tctgtttttt gaaaactacc cctttcgggt gatttttttt tgcatttttg ctgctataat
     1741 attactaaaa acaatttatc attaagaaga tgaggcttgc cttcactcag gatgataaac
     1801 ataatataag gtctgtaacg gataaacaat ataagatgcg gtggagatta tgaatgcaat
     1861 cagaaaaaga ataattatct acatttcaat aatatgcttt ttaatagctg catgggctca
     1921 gcttttttcc cttcaaggcg gaaactttaa tgctcaaacc tgtgttataa caataatcac
     1981 agtagccgga gggattatac cgatatacct tttatttaag gatattaata atttgaaaat
     2041 agcaaggctg ataaaggaaa acttcatatt gaaagtcaaa actgcagtta taagcgaaat
     2101 atcagaaaaa gaaattcctt ataaaccaat aaacatagaa gatactgaaa tatttgtatc
     2161 atattttgga attcttttta acggtaaaat cgcaaaattt aatcaagacg gcatacggct
     2221 tacagcaatg gatataggaa atgattatat atcatttact tacggcaatg aaaaacaaat
     2281 gaaaaacata cgtattgtgc gccccgatat tgagcctgat gccatgagtg aaatgattaa
     2341 taaatttcgt tttgaaatgg ggattacacc cacaatggtc ggtaagataa ttacaaataa
     2401 atgtaacaat gaggtgaaat atggctaaaa aatttgttgg ttatggaagc tttttttgct
     2461 ggaattgcgg taaaggaatt gaaagagggg aaa
//
LOCUS       DAOPUE010000187         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_45843_length_2427_cov_7.261383, whole genome shotgun sequence.
ACCESSION   DAOPUE010000187 DAOPUE010000000
VERSION     DAOPUE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45843_length_2427_cov_7.261383"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKH42_10125"
     CDS             <1..420
                     /locus_tag="PKH42_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOA20833.1"
                     /translation="DGTFRPSDPITRAEFATIAAKFDDLDLGSASKFSDIFGHWAEKY
                     ITSAENKGWINGYPDMTFKPEQDITRAEAMTLINNVLERAVPAENIHSDAMFWPDIDE
                     DDWYFEAIMEATNSHDYVIEEDGDELWTGMKPNKVWP"
     gene            980..1861
                     /locus_tag="PKH42_10130"
     CDS             980..1861
                     /locus_tag="PKH42_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOA20834.1"
                     /translation="MVYYVEYIFVENFIIDFILLFITGKLVKRAIIFKRLIAASLIGS
                     LYVIVTLYAGKEFMNNFIVKFSISVLMIMVAYDSKGILTNIRVIICFYITSLIMVGII
                     SALYILTYDKLTVNAIIMSIFTGYAALHFFFKEIKDRIVKHNYKRKVNVSICGKSKTF
                     RGYIDTGNELTDPITGKPVMVVNIDCIKEILGDELCNKIMEFYENKSYEKLIDENNSI
                     NLRVIKYKTISNSGENMVCIVPDEIEIINNNKSILVDAIIGIHPKRISMDDYDALLFK
                     KLLDWEVETENEYVDAC"
     gene            1839..>2427
                     /locus_tag="PKH42_10135"
     CDS             1839..>2427
                     /locus_tag="PKH42_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOA20835.1"
                     /translation="MNMLMHVKTFFKKIINFIIDKLGIIKDIFYIGGSDTLPPPLTQE
                     EEKEIISRLKDSNEAKTILIEHNLRLVVYLAKKFESTGINVEDLISIGTIGLIKAVNT
                     FKAEKNIKLATYASKCIENEILMHLRRVGKTKTEISLDEPLNIDWDGNELLLSDILGT
                     EEDVVFRNMENEVDLSLLNDSIQKLNKREYMIMKLR"
BASE COUNT          881 a          322 c          485 g          739 t
ORIGIN      
        1 gacggaacat tcagaccgtc agacccgatt acgagagcag aatttgcaac tattgcagct
       61 aaatttgatg atttggactt aggaagtgca agcaaattct ctgatatatt cggacattgg
      121 gcagaaaaat atattacttc tgctgaaaac aagggatgga ttaatggtta tccggacatg
      181 acatttaaac ctgagcagga tatcacgaga gctgaagcaa tgacattgat taacaatgtt
      241 cttgaaagag ctgttcctgc agaaaacata cattccgatg caatgttctg gcctgatatt
      301 gatgaagatg actggtattt tgaggctata atggaagcaa ccaacagtca cgattatgtt
      361 atcgaggaag acggcgatga gctttggacc ggaatgaaac cgaacaaggt atggccgtaa
      421 aggaatatcc aaaaaaagta ataacattta aataatagcg gggctaaatg ccccgcttgt
      481 ttatgacaaa ggatacaaaa tctaatgttt tgcctaagct aggtatgaca actttctctt
      541 tttgtcattc tgagcaagcg aagaatctca aggcatcttt cacttgcgct cggtatgaca
      601 gctctctctt tctgtcattc tgagagcttt agctcgaaga atctcaaagg cccctacgct
      661 atggatgaca taaagtggtt gtgtacaaca taaacttatt aagtatattg tagaagggtt
      721 caaaaaaagc agaaaaatgt aaagcataat ttttgcggcg aactatacga aatacgctat
      781 agtattcata aaaagcgtat ttttttattt ttgattacat gttatgacaa aatatttcga
      841 taaggtcatt tataattgtt aatagattgc ttggctatag aaacaaggga cgatgggtga
      901 gatgcttcag gaaaagactc aggatgacaa attaatcatt ctgaaccgca gatgaagaat
      961 ctcgagggag ttggaaggct tggtttatta tgttgaatat atttttgtcg aaaattttat
     1021 catagatttt attttgcttt ttattaccgg aaagcttgta aaaagggcaa taatattcaa
     1081 aagacttatt gctgcatctt taatcggctc attgtatgtt attgttactt tatatgcggg
     1141 aaaggaattt atgaacaatt tcatcgtaaa attcagtatt tccgtattaa tgataatggt
     1201 agcatatgat tccaaaggga tattgactaa tatcagagta ataatttgtt tttacattac
     1261 ctcattgata atggtgggaa ttatttccgc cttatacatt ctaacatatg ataaattaac
     1321 cgtgaatgca attatcatgt ccatatttac agggtatgct gcattgcatt tcttttttaa
     1381 agaaattaaa gacagaattg ttaagcataa ttacaaaaga aaggtgaatg tcagcatatg
     1441 cggtaaatcc aagacattca gagggtacat tgatacgggc aatgaattga cagatccgat
     1501 taccggaaaa cctgttatgg ttgtaaatat tgattgcata aaagaaattt tgggagatga
     1561 gctatgcaac aaaattatgg aattttacga aaataaaagc tatgaaaaat taattgatga
     1621 aaacaacagc attaatttaa gagttattaa atacaaaact ataagcaaca gcggtgaaaa
     1681 catggtttgc atagtgccgg atgaaataga aataataaat aataataaaa gtattttggt
     1741 tgatgcaatt ataggaatac atccgaaaag aatcagcatg gatgattacg atgcgctttt
     1801 gttcaaaaaa ttacttgatt gggaggtgga gacggaaaat gaatatgttg atgcatgtta
     1861 aaactttctt taaaaaaata attaacttta taatagataa attaggaata attaaggata
     1921 ttttttatat cggaggaagc gatacattac ctcccccctt gacacaggag gaagaaaaag
     1981 aaataataag cagactaaaa gattctaacg aggcaaaaac aattttaatt gagcataatt
     2041 taaggcttgt tgtatacctt gcaaagaagt ttgagtctac cggaataaat gtggaggatt
     2101 tgatttccat aggaactatc ggactaataa aagctgtgaa tacatttaag gcagaaaaaa
     2161 atattaagct tgcaacttat gcttctaagt gtattgaaaa tgaaatatta atgcatttaa
     2221 gacgggtagg aaaaaccaag acggaaattt ctttagatga gcctttgaat attgattggg
     2281 atggaaatga gcttttgctg tcagatattt taggtacgga agaagatgta gtatttcgta
     2341 acatggaaaa tgaagtggat ttaagtcttc ttaatgattc aattcaaaaa ttaaataaaa
     2401 gagaatatat gattatgaaa ttaaggt
//
LOCUS       DAOPUE010000188         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_49053_length_2318_cov_8.589041, whole genome shotgun sequence.
ACCESSION   DAOPUE010000188 DAOPUE010000000
VERSION     DAOPUE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49053_length_2318_cov_8.589041"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PKH42_10140"
     CDS             complement(<1..832)
                     /locus_tag="PKH42_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOA20836.1"
                     /translation="MKKLDLRLFRLIKTTKGQYIAVLSIIITGIFIFTAVSNSAINLR
                     DSLNDYYYTTNFADIFVTAAAIPEKLEKELEATWNIRQADARLSIDTRFITDNDDERV
                     DVRAVSVDTNENKINELFIKKGKRTISDREIIVIEQFALAREIDTGDEIVLNINGRNH
                     RFIVSAIASSPEYVYLMENEQVLLPDYENFGVVYIEEDYLRKISGRGSFNEVVIRVDN
                     QDNIDKTKDYLEDGLKKYGVMRVIGRDDQLSNSMMNEEITGLEKVSQSIPIIFLLFAG
                     I"
     gene            complement(832..1539)
                     /locus_tag="PKH42_10145"
     CDS             complement(832..1539)
                     /locus_tag="PKH42_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOA20837.1"
                     /translation="MGQKVLKVENLSKYYQMGEIAVKALEDVSFEVEKGEFVVILGPS
                     GSGKSTLLNIMGGMDTPSGGSVLFNDELITNYTDKELTMYRRHKIGFVFQFYNLMATL
                     TAKENVELVAEITDSSVNIDEVMEAVGLGDRTDHFPSQMSGGEQQRVAIARAVAKAPQ
                     ILLCDEPTGSLDFETGIQILKVLKDVNVKYKNTVIVITHNASIAQMADKVIKMRSGSI
                     TEIIENAAPIEPERIEW"
     gene            complement(1871..2305)
                     /locus_tag="PKH42_10150"
     CDS             complement(1871..2305)
                     /locus_tag="PKH42_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOA20838.1"
                     /translation="MAKVLAVNISEKKGIVKKPIDKGYFEADHGLVGDAHAGNWHRQV
                     SLLGQESIDKINKQDIKGLCVGVFAENLTTEGIILYELPIGTKLKIGETEMEVTQIGK
                     ECHTGCAIRKLVGDCVMPREGIFTKVLKAGWIKPGDEIEVID"
BASE COUNT          639 a          533 c          353 g          793 t
ORIGIN      
        1 ttatgccggc aaaaagcaaa aatatgatgg gaatggactg agataccttt tcaaggccgg
       61 taatctcttc attcatcatg ctgttgctca gctgatcatc tctgcctata accctcatca
      121 caccatattt tttaagcccg tcctctaaat aatcctttgt tttatcaata ttatcctgat
      181 tgtctactct aattaccact tcattaaaag atcctctgcc ggatatcttt cgcagataat
      241 cttcttcaat ataaacaact ccgaagtttt cataatccgg tagaagtacc tgctcatttt
      301 ccatcaagta cacatattcc gggcttgaag caatcgcaga tactataaac ctgtggttcc
      361 tgccgtttat attaagaact atttcatccc cggtatcaat ttcccttgcc agggcaaact
      421 gctcaataac aataatttct ctatcagaaa tagtccgttt tccttttttt ataaataatt
      481 cattaatttt attttcattt gtgtcaacag aaacagccct tacatccact ctttcatcgt
      541 cgttgtcggt gataaatctt gtatctatgg acagccttgc atctgcctgc cggatattcc
      601 aggttgcttc caattccttt tctaactttt caggaatggc agctgccgtt acaaaaatat
      661 cagcaaaatt agtagtataa taataatcat tcaggctgtc ccttaaattt atggcagaat
      721 tgctgacagc tgtgaaaatg aaaatacccg taataattat tgacaaaacg gcaatatatt
      781 gaccctttgt agttttaatg agtctaaaaa gtcttaaatc cagctttttc attaccattc
      841 aatcctttca ggttcaatgg gagctgcatt ttcaataatt tccgttatac tgccgcttct
      901 cattttgatt accttgtcag ccatttgagc aattgacgca ttatgagtaa ttactattac
      961 agtattttta tattttacat taacgtcctt tagaaccttt aatatttgaa ttcctgtttc
     1021 aaaatcaaga gaacctgtgg gttcgtcgca cagtaaaatc tggggagcct ttgccacagc
     1081 tcttgctatg gcaaccctct gctgttcacc gccgctcatt tgagatggaa aatggtcggt
     1141 tctgtcaccg aggcccactg cttccatcac ttcgtcaata tttactgaac tgtcggttat
     1201 ttcagctacc agttccacat tttccttagc agtgagggtg gccatgaggt tgtaaaactg
     1261 gaaaacgaat cctattttgt gccgcctgta catggtaagt tctttatcag tatagttcgt
     1321 aatcaattca tcattaaaga gcacggagcc gccggaaggt gtatccatgc cccccataat
     1381 gttaaggagg gtgcttttgc ccgagccgct gggacctaaa ataactacaa attcgccttt
     1441 ttcgacttca aagcttacat cttccaaagc tttaaccgct atttcgccca tctgatagta
     1501 cttgcttaga ttttcgacct ttaatacctt ttgacccata gtttccccca aatattaaac
     1561 tgtgaaccat attaaaactg tattatatca taagtacaag cagtaaaatc ggcttgcttt
     1621 gcaccgatac aattaataca ctgaggcaaa gacacaggtc tttgcttcat taattgtatt
     1681 cattataaca taatttggaa cataaataaa tatattttaa tctagactat tcgctacaaa
     1741 aaacctcccc gaaagaatac tttcactccc tcagcggtgt tatccgctgt aatgaaggag
     1801 tcgtcatcat atctttacat ataaaaaaac tgttacatta cagcaacagt catatattat
     1861 ctaactttaa ttaatctatt acctctatct catctccggg ttttatccat ccggctttca
     1921 gaacctttgt aaatatccct tccctgggca taacacagtc gcctacaagt tttcttatgg
     1981 cacaccctgt atggcattcc ttgcctattt gagtgacttc catttctgtt tccccgattt
     2041 tcagttttgt acctattgga agctcgtata aaataattcc ttcagttgta aggttttctg
     2101 cgaagacgcc tacgcacaaa ccttttatat cctgcttgtt aattttatcg atactttcct
     2161 gacccaataa acttacctgt ctgtgccagt ttcctgcatg ggcatcccct acaaggccgt
     2221 gatctgcttc aaagtaaccc ttatcgatgg gctttttcac aatccctttt ttttcgctta
     2281 tatttacagc taatactttt gccattttat tctccatt
//
LOCUS       DAOPUE010000189         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_53616_length_2186_cov_10.349601, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000189 DAOPUE010000000
VERSION     DAOPUE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53616_length_2186_cov_10.349601"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1724
                     /locus_tag="PKH42_10155"
     CDS             24..1724
                     /locus_tag="PKH42_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOA20839.1"
                     /translation="MKKILRINLRTKKITYQDASREYLETGGRGLIAKIMLNEVNPLC
                     DPLGRENKLIIANGLLTGTNVSSASRIAIGAKSPLTGGIKESNGGGITAMWLASLGIK
                     ALVVEDIPEENEWYYIRINKDNCSLEPAGEYKGMGTFEFCEKMLDKYPDCAVTCIGQA
                     GERLYNIAGIATMDKEKRPNRYSGRGGLGAVMGSKKIKAIVVEEKGTVEIKNKEKYNK
                     ILKEYSAIVLNAPTSVSYKTFGTAAMVRNANDLTGLPVTNFKGAKFEHAEEISGQSLY
                     ELIKERGGEGKTAHACMPGCIIQCSNVVPDEEGKTIVSPLEYETIGLMGSNLGIGDLD
                     TIAKLNAVCNDLGVDTIETGAVMGMAMEAGIVEFGDNEAALNLLDEIKTGTELGKLLG
                     EGTLIAGKALGVKNIPVINGQAMPAYDPRAIKAMGVTFATSPMGADHTYGPVVKAEDA
                     VEASKAAQIMMAKIDCLGVCMFLRGAVAKHMDLFVDLVNAVHGWDLDMDWLEDIALEA
                     IRDEHKFNELAGFTKEDYRMPKAFTNREIDSTNQVFDIDKDELDELRLWHDKNKIIYR
                     "
     gene            1696..1908
                     /locus_tag="PKH42_10160"
     CDS             1696..1908
                     /locus_tag="PKH42_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOA20840.1"
                     /translation="MIKIKLSTVDSDVENIDLDYKDITIDEIITELKIDKQHIGAVLV
                     NGIPKKFSDKVEDNSEVFFLPILSGG"
     gene            complement(1951..>2186)
                     /locus_tag="PKH42_10165"
     CDS             complement(1951..>2186)
                     /locus_tag="PKH42_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOA20841.1"
                     /translation="GMGWMSVLVFMPLVRSMETGGIIWLCLGGLLYTAGGIIYGLKKP
                     AINKQWFGFHELFHIFVLAGSFCHFMMMYFYVA"
BASE COUNT          780 a          351 c          506 g          549 t
ORIGIN      
        1 gacattttaa ataggagaac aatatgaaaa agattctgag aataaattta agaacaaaaa
       61 aaattactta ccaagatgct tctcgggaat atttagaaac aggaggcagg gggctaatag
      121 caaaaataat gctaaatgaa gtaaatcctc tatgcgaccc cttaggcaga gaaaataaac
      181 ttattattgc caacggacta ttgaccggta caaatgtgtc cagtgcaagc agaattgcta
      241 tcggagctaa aagtccctta acaggcggaa tcaaggaaag caacggagga ggaattacag
      301 ccatgtggct tgcttccctt ggaataaaag ctcttgttgt tgaagatatt cctgaagaaa
      361 atgaatggta ttatataaga atcaataagg ataattgttc cttagagcct gcaggagagt
      421 ataaaggaat gggaaccttt gaattttgcg aaaaaatgct tgataaatat cctgactgtg
      481 cagttacatg cataggtcag gcaggcgaga gattgtacaa tattgccgga atagcaacta
      541 tggataagga gaaaagacct aacagatata gcggcagggg cggtcttggg gctgtgatgg
      601 gctcaaagaa gataaaagca atagtagtag aagaaaaagg gacggtagaa ataaaaaata
      661 aagaaaaata caacaaaata cttaaggaat attcagcaat tgttttgaat gctcctacat
      721 ctgtttcata caaaactttt gggacagctg caatggtaag aaatgcaaat gatttgacag
      781 gacttccggt aacgaacttc aaaggtgcaa aatttgagca tgcagaagaa atttcagggc
      841 agagcttata tgagcttata aaagaaaggg gcggagaagg aaagactgct catgcatgta
      901 tgcctggctg cataattcaa tgctctaatg ttgttcccga cgaagaagga aaaacaatag
      961 tatctccatt ggaatatgaa acaatcggat tgatgggctc caacctcgga ataggagatt
     1021 tagacacaat tgcaaaacta aatgcagtct gcaatgattt aggagttgac acaatcgaga
     1081 caggagctgt catgggaatg gcaatggaag caggtattgt ggaatttggc gataatgaag
     1141 cagctttaaa tttgcttgat gaaataaaaa ccggtacaga gctcgggaaa ctgctcgggg
     1201 aaggaacact tattgcggga aaggcactgg gagtaaaaaa tatccctgtt ataaacggtc
     1261 aagcaatgcc tgcttatgac ccgagagcca taaaggcaat gggagttaca tttgcaacat
     1321 caccaatggg agcggaccat acttacggtc ccgtggtaaa ggcggaggat gctgtcgaag
     1381 catcaaaagc agcacaaatt atgatggcaa aaatagactg tttgggagta tgtatgtttt
     1441 taagaggagc tgttgcaaaa catatggacc tattcgtgga cttggtaaat gcagtgcacg
     1501 gctgggattt agatatggat tggcttgaag atatagctct cgaagctata agagatgagc
     1561 ataaatttaa tgaacttgca ggttttacca aagaagacta tcgtatgcca aaagcattta
     1621 caaacaggga aattgatagt acgaatcaag tttttgatat tgataaggat gaactggatg
     1681 agttgaggtt gtggcatgat aaaaataaaa ttatctaccg ttgacagcga tgtggagaat
     1741 attgatttag actataaaga tattacgatt gatgaaataa ttacggaatt aaaaattgac
     1801 aagcagcaca taggtgctgt gcttgtcaac ggtattccaa agaaattctc agataaagtc
     1861 gaagataatt cagaagtatt cttccttccc atactgagcg gcggctagaa gcctgtgtca
     1921 tttgcttacg ctatggatga ccttaataaa ttaagctaca taaaaataca tcatcataaa
     1981 gtgacagaag gaacctgcta atacaaatat atgaaacaat tcgtgaaacc cgaaccattg
     2041 tttatttatg gcgggttttt ttaaaccgta aattattccg ccggctgtgt ataatagtcc
     2101 cccgagacaa agccagatga ttccgcctgt ttccatgctt cttaccaagg gcataaaaac
     2161 aagtactgac atccatccca tgccta
//
LOCUS       DAOPUE010000190         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_54024_length_2174_cov_15.445021, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000190 DAOPUE010000000
VERSION     DAOPUE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54024_length_2174_cov_15.445021"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1375)
                     /locus_tag="PKH42_10170"
     CDS             complement(68..1375)
                     /locus_tag="PKH42_10170"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HOA20842.1"
                     /translation="MKWFYELLSLSLSVTPIILLLLCLNPILKKRYSAKWRYTLWIAV
                     SICLLLPFSYVKNFIAPLKVTETVPSELLPSTSVAVGQMPKINTISLYNANDSFGKVL
                     IIIYFIGVILYLAYVIFSYIGFRRDIFRWSKKTTSHEIKTILNDEKNRLNIKRKVPLL
                     ICKKASSPMLVGLIKPILVLPSETYRPEELRMILIHELVHYKRNDILIKTILIAASVV
                     HWFNPVVHLMVKQANKDMEQSCDDYVLNGCDVKEKKFYCNIILKMAILNNNEAVHVFS
                     TNIVSSRKNLESRIKGIFDSSRKKRGIITLIAVILFVIISGTIFNVTGAAEPEKISLE
                     NEFSDKQNDVINENNAGNENQVKAQIEENEFEIYNKFENSEPETLPATDNIQQDNTQQ
                     IEINQEGSIKTDEEAMVENDMSEIVIVDLNQLEGQLKERETVE"
     gene            complement(1372..1752)
                     /locus_tag="PKH42_10175"
     CDS             complement(1372..1752)
                     /locus_tag="PKH42_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOA20843.1"
                     /translation="MDTIKRIPDSELEIMMIIWEANEPVSSTYIIEKLKGEKDWAHTT
                     VLNFLARLVDRGFLETSKQGRFNYYTSIISEKDYLQKESKTFLEKMHKNSLKSLVAAL
                     YDGDAISKEDLEELSNFVDEVSKS"
     gene            complement(1907..2044)
                     /locus_tag="PKH42_10180"
                     /pseudo
     CDS             complement(1907..2044)
                     /locus_tag="PKH42_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
BASE COUNT          665 a          391 c          297 g          821 t
ORIGIN      
        1 catcaatata actaaaaaca aaaatatctt tcttttcatt attgttcctc cttaaacaca
       61 taagttacta ttcaacagtt tctctttcct taagctggcc ctccaactga tttaaatcaa
      121 caataacaat ttccgacatg tcattttcaa ccatagcttc ttcatctgtt tttattgaac
      181 cttcttgatt aatttctatt tgctgtgtgt tatcttgttg tatattatct gttgccggca
      241 gggtttccgg ttctgagttt tcaaatttat tgtatatttc aaattcattt tcttcaattt
      301 gagcttttac ttgattttca tttccagcat tattttcatt tataacatca ttttgtttgt
      361 ccgaaaattc attttcaaga gatatttttt ccggctctgc tgcaccggtg acattgaata
      421 ttgtgccgga tataataaca aataatatta ctgcaataag tgttataata cctctctttt
      481 tccttgagct gtcaaaaatg cctttgattc gggactcaag attttttctg ctgctgacaa
      541 tattggtgga aaaaacatgt accgcttcat tgttatttaa tatagccatt ttgaggataa
      601 tgttgcagta gaattttttc tccttaacat cacaaccgtt caatacataa tcatcacaag
      661 actgctccat atctttgttt gcttgtttta ccattaagtg cactacaggg ttaaaccaat
      721 gaacaacgga agcagcgatt aatatggtct taattaaaat atcattcctt ttataatgta
      781 ccaattcgtg tatcaatatc attcttaatt cttcaggcct gtatgtttct gaaggcagca
      841 ctaatattgg ttttataagt cctaccagca tggggcttga tgctttttta catatgagta
      901 gaggtacttt tcgttttata ttaagtctgt ttttctcatc attaagtatt gttttaattt
      961 catggcttgt tgttttttta ctccatctga aaatatcacg tctaaatcca atgtaagaaa
     1021 atattacata agccaagtat aatatgactc cgataaaata tattataatc aaaacctttc
     1081 cgaagctatc gtttgcgtta tataagctaa ttgtgtttat ttttggcatt tggccaacag
     1141 caacagaagt tgaaggcaac agctcgcttg gtacagtctc agtaactttc aagggtgcaa
     1201 taaaattttt tacataggaa aacggcaata gcaagcatat agaaacagct atccacaagg
     1261 tataccgcca ctttgcagag tatctttttt tgagtatggg gttaaggcat aacaacaata
     1321 gaattatagg agttacactt aagcttaatg ataataattc atagaaccat ttcatgattt
     1381 tgatacctca tccacaaaat tacttaactc ctccaaatct tccttgctta ttgcatctcc
     1441 gtcatatagc gcagcaacaa ggcttttcaa ggaatttttg tgcatctttt caagaaaggt
     1501 cttgctttcc ttctgcaagt aatccttttc acttataata gaagtataat aattaaacct
     1561 tccctgcttt gatgtttcta aaaatcctct atccaccaat cgggctaaaa aatttaaaac
     1621 agttgtgtgg gcccaatcct tctccccttt taacttttca attatatatg ttgatgatac
     1681 cggctcattt gcttcccaaa ttatcatcat tatttccaat tcactgtcgg gaattctctt
     1741 aatagtatcc atttccaact caatcctctc aaaaaattca aataatatta ttaaacttat
     1801 tctacaatcg tagaatgagt ttgtcaagga ttatttcaga aacattaaac ttagttttcc
     1861 ctttcatatt tatataattt attctctgaa gatttaccat cttcactatt tcatccggcg
     1921 aattccattt tttaagatat gcagcaagtc ctgtatccaa aaaatataat ttaggtgttt
     1981 tgacagctct tttaattata ttgttatagt acggcttaag caaatttgca atattagatg
     2041 aaatactaaa aggatttaaa aaagaaagaa taaaagcttt agattttcag caattatctg
     2101 caaattctta atggcggtgt aatatttaat gtatcaggct ctattataaa taaagtcttt
     2161 tttttagatt tagc
//
LOCUS       DAOPUE010000191         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_54094_length_2172_cov_11.427019, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000191 DAOPUE010000000
VERSION     DAOPUE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54094_length_2172_cov_11.427019"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1286
                     /locus_tag="PKH42_10185"
     CDS             177..1286
                     /locus_tag="PKH42_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOA20844.1"
                     /translation="MTYIIYTVQPGDTLYSIARMYGSTIQGIIDTNNITNPNLIYPGS
                     VILIPVEEEYQETPPGSLIYTVQPGDTLYIISLLFKVSIQRILELNHIPDPSLIFPGM
                     KIVLPSEAVNPFQPVVPGIIRYTVLPLDTIYKIAARFGTTTQLILNANPGLDPRRLFP
                     GMVITITIPENAVAIYRGNPNRRMVSLTFDATYGDNQTYELLEILRNNNIKATFFLSG
                     IWLINYPELARAIAAEGHEIGNHSFTHPHMPLITLQEVTNQIIRTEALIRNITGQDPY
                     LFRPPYGEYTQAMLNQLASLGYVTIMWTIDSLDWQNPGADAVIARVVNNAEPGAIILL
                     HQSAPDTLNGLQNMITQLRQGGYDFGTVTQVISPL"
     gene            complement(1361..>2172)
                     /locus_tag="PKH42_10190"
     CDS             complement(1361..>2172)
                     /locus_tag="PKH42_10190"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOA20845.1"
                     /translation="HTGGATMGSDHLIWLKDDGTYEVLDSGYSLMKMYDDYTVRVEQR
                     FSFNVDNLQIKKTGEKDYTNIGNPDYSYGMYIHETGSGNNKSRGGSPSKDLYLINDEI
                     YVLGYYGYYLEENNAATTGIYRVNINSNEQVRLCDNEVTSFKILDDTIYFADQSNLIY
                     KVSLAGGKAELLVDEAVNSYEVLEGKLYYSLQENNQLYIYGNVESVNPGGILKSLEVQ
                     NGYMVAIFDKLSESQYKLMIFNEEGKVLYKSIENVLLVRIDNGKVVFVKDN"
BASE COUNT          702 a          436 c          365 g          669 t
ORIGIN      
        1 atattatatt acatatccga attatttcaa cataatttgt atgtcaggga gacgtatgac
       61 aaggagacgg ggtgccatat gacaagtaga cggggatgtt gaagttaatt tataattatc
      121 actttgtttg agttatggaa tattatatga ataaagaata ggaggaccat tataatatga
      181 catatataat atacacagta caacccggag atacactcta tagtatagct cgtatgtatg
      241 gcagtacaat acaaggaatt atagacacaa acaacattac aaatcccaac ttgatttatc
      301 ccggctctgt tatattaata cctgttgaag aggaatacca ggaaacacca cccggaagcc
      361 ttatctatac tgtgcagccc ggtgatactt tatatattat ttctcttttg ttcaaggtaa
      421 gcattcaaag aattttagag ctaaaccaca ttcctgaccc ttcattgatt tttccgggaa
      481 tgaaaattgt attgccgtca gaagccgtta atccgtttca gcctgtagtg cccggtataa
      541 tcaggtatac tgtgctgcct ttagatacca tctataaaat cgctgcaaga tttggtacaa
      601 caactcaatt gattttaaat gctaatccag gattagaccc tcgcaggctt tttcccggaa
      661 tggtcataac aattacaata ccggaaaatg cagttgccat atacagggga aatccaaata
      721 ggaggatggt atctttaaca ttcgatgcaa cttacggcga taatcaaaca tatgaattgc
      781 tggaaatatt aagaaacaat aatataaagg caacattttt cctatctgga atctggttga
      841 taaactatcc tgaattagca agagctatag cagctgaagg tcatgaaata ggaaatcaca
      901 gctttaccca ccctcatatg ccccttataa cattgcagga agtcacaaac caaattatta
      961 ggacagaagc tcttataaga aatatcacag gtcaagaccc gtatttgttc aggcctccat
     1021 acggcgaata cacgcaggca atgctaaacc aattggcttc actgggctat gtaacaatca
     1081 tgtggaccat tgactcttta gactggcaaa atccaggggc tgatgcagta atagcaagag
     1141 ttgtaaataa tgcggagcca ggagcaatta tcctgcttca ccaatcagct ccggatactc
     1201 ttaacggact gcagaatatg ataacgcaat taagacaagg aggatacgat ttcggtacag
     1261 tcacacaggt tataagcccg ctataaatga caaggggacg gggggaatgt cataaacaca
     1321 tgactgcccg cgtcctcttt aatttactta aataattcca tcaattatct tttacgaaaa
     1381 caaccttacc attatcaatt cgcactaata atacattttc aatggattta tataatactt
     1441 ttccttcttc attaaaaatc atcaatttat attgagattc gctaagttta tcaaatatgg
     1501 caaccatgta gccgttttga acttctaaac tttttagaat gcctccggga tttactgatt
     1561 ctacattacc atagatataa agctgattgt tttcctgcaa tgagtaatat agttttcctt
     1621 ccaatacttc atatgaattt accgcttcat ctaccaaaag ctctgccttg ccgccggcaa
     1681 gcgaaacttt ataaataaga ttactctggt cggcaaaata tattgtatca tcaagaattt
     1741 tgaaacttgt tacttcatta tcacacaacc tgacttgttc attgctattt atgttaactc
     1801 tgtaaatacc tgttgttgca gcattatttt cttccaaata atagccgtaa taccccaata
     1861 catatatttc atcatttatt aaatacaagt ctttacttgg actcccgccc cgtgacttat
     1921 tgttacctga cccagtctca tgaatgtaca tgccataaga atagtctgga ttacctatgt
     1981 ttgtataatc tttttcaccg gtctttttta tctgcaaatt atctacatta aatgaaaatc
     2041 tttgctcaac tcgcacagtg taatcatcat acattttcat aagggaatag ccgctatcaa
     2101 gaacttcata ggtaccgtca tcttttagcc atataagatg atctgaaccc atggttgctc
     2161 cgcctgtatg gt
//
LOCUS       DAOPUE010000192         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_54849_length_2153_cov_8.312202, whole genome shotgun sequence.
ACCESSION   DAOPUE010000192 DAOPUE010000000
VERSION     DAOPUE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54849_length_2153_cov_8.312202"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..853)
                     /locus_tag="PKH42_10195"
     CDS             complement(89..853)
                     /locus_tag="PKH42_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOA20846.1"
                     /translation="MIKNKLKEKLNAGKAAIGTFIVCNAPDLVEIAGLAGFDFVIIDN
                     EHGPMRAESSQHLIRAAEVRGITPIVRIPDRQESTILHTLDIGAHGIQVPQVNDVEAA
                     KEIIERSKYQPVGKRGLAFPRSADYGLCDISNYTDNANKETLIITHCENKTCLDNLEE
                     ICKIPEIDVIFLGPYDMSQSMGVTGQVTHELVESAAEKVLEVTKRYNKIAGIFAGNGE
                     TARKRAEQGFLYIAVGMDTTLFSAKCIEEISKFHNC"
     gene            complement(850..2064)
                     /locus_tag="PKH42_10200"
     CDS             complement(850..2064)
                     /locus_tag="PKH42_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOA20847.1"
                     /translation="MSKYAKRMKTMEEPANIIKGLFSSMNDPGVISFGGGAIAKECLP
                     VDIVREITSDIMTVEKRGYEILSYGPVIGVKDLRDVIVKYLLAPKGVKANADNIIITT
                     GGLEAMSLMCQLFIEPGDVILVESPTFVHSVETFSMFEAKCVAVEMDDDGMVTEELEE
                     KIIKYNPKIVYVIPTFQNPTGRTLSLERRQKIAELGSKYDVIILEDDPYRDIRYSGED
                     LLPIKAFDNTGHTVLANSFSKIFSPGSRIGYVYANDDIISKMTDAKSASNSHTSLLPQ
                     IICAEFFKRGYYPEHHKLLCDIHRERRDAMLESIDKFFPEGTKRTFPDGGLFTWVELP
                     GGINTSELLKESVSNPEVKVAFVAGEGFFTEGKGKGSNCMRLSFGANPPDKIRIGIER
                     LGNLIKSKLEEK"
BASE COUNT          586 a          498 c          326 g          743 t
ORIGIN      
        1 gggatattcc ctttatgaga tccttcgctt acgctcagga tgacaaggca aagcttacgt
       61 tcaggatgcc gatacagtaa aaaatatttt aacagttatg aaacttactt atttcttcta
      121 tacatttagc actgaataat gttgtatcca tgcccacagc tatatacaaa aatccctgct
      181 ctgcgcgctt tcttgcagtt tctccgtttc ctgcaaaaat ccccgctatt ttattgtatc
      241 gttttgttac ttcaagaacc ttctcagccg cactttcaac taattcatga gttacctgtc
      301 ccgttactcc cattgattga gacatatcgt atggtcctaa gaatataaca tctatttcag
      361 gaattttaca tatttcttcg aggttatcca aacatgtttt attttcgcag tgagtgatta
      421 taagagtttc tttatttgca ttatcggtat agttagaaat atcgcacaag ccgtagtcag
      481 ccgaccttgg aaaagccaaa cctcttttac ctacaggctg gtacttgctt ctttctataa
      541 tttccttagc agcttctacg tcattaacct gcgggacctg aatcccgtga gctccaatat
      601 cgagggtatg aagtattgtg ctctcttgcc tgtcaggaat tcttactatt ggagttattc
      661 ctcttacttc cgctgctcta atcaagtgtt gagagctttc tgccctcata ggaccgtgtt
      721 cattgtctat tattacaaag tcaaaccctg ccaaacctgc tatttcaacc aaatccggag
      781 cgttgcacac aatgaacgtt ccaattgccg cctttccggc atttaacttt tctttgagct
      841 tattctttat catttttcct ccaatttaga tttaattaaa tttcccaatc tttcaattcc
      901 aattcttatt ttatcgggag gattggcacc aaaacttaat ctcatgcaat tactgccctt
      961 tcccttacct tcggtaaaaa atccctctcc ggcaacaaag gcaaccttaa cttcaggatt
     1021 tgaaacagat tcttttaata attctgaagt atttattcca ccaggcaatt ccacccatgt
     1081 gaataaccct ccgtcaggaa atgttctttt agttccttca gggaaaaatt tgtctatact
     1141 ttcgagcatt gcatctcttc tttctctgtg aatatcgcac agcagcttat gatgttcggg
     1201 gtagtatcct ctcttgaaaa actcggcaca aattatctga ggcaacagcg aggtatgtga
     1261 atttgaagct gactttgcat ctgtcatctt tgatattata tcatcatttg catacacata
     1321 tcctattctg cttcccggcg aaaatatctt cgaaaaacta ttagccagca cagtgtgacc
     1381 agtattatca aatgctttaa tcggcaataa atcttcgccg ctgtacctta tatcccggta
     1441 tgggtcatct tctaatataa ttacatcata cttgcttcca agctctgcaa ttttttgtct
     1501 tctctccaag gataaggtcc tgcctgtagg attttggaat gtaggaatta catatactat
     1561 ttttggattg tatttaataa ttttttcctc cagctcctcg gtaaccatcc catcatcatc
     1621 catttcaaca gcaacacatt ttgcttcaaa catgctgaat gtttcaacag aatgaacgaa
     1681 ggtaggagat tccaccaaaa ttacatcacc cggctcaatg aaaagctgac acattaagct
     1741 cattgcttcc aatccgccgg ttgtaattat aatattgtcg gcatttgcct tgaccccttt
     1801 tggagccagt agatacttta ctattacatc tcttaaatcc ttaacaccaa ttacaggtcc
     1861 gtaggacagt atttcataac ctcttttctc aactgtcatt atatcagagg taatttctct
     1921 tactatatca acaggcaaac attcctttgc aattgccccg ccgccaaagg aaatcacacc
     1981 gggatcgttc atggagctga ataaaccttt tataatattg gccggctcct ccatagtttt
     2041 cattcgtttt gcatattttg acatattatt tctccttaat tcttattatt atccttaaca
     2101 gtaatcttcc gtgagcatta ttaaatttct taaattatac catcaaaaat tac
//
LOCUS       DAOPUE010000193         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_56174_length_2118_cov_12.063015, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000193 DAOPUE010000000
VERSION     DAOPUE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56174_length_2118_cov_12.063015"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKH42_10205"
     CDS             complement(<1..1127)
                     /locus_tag="PKH42_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOA20848.1"
                     /translation="MKNKKLVMIPGPTPTVRTINDQMGRETVAFGDPVFVKDFSELIV
                     DLKEMWRVDGECFVVAGSGTMAMEMAVANVTKRDDNILIVSNGFFGDRFIEICNRKGL
                     NVDVLSAEWGDVVSPEAIEEKLKEKNYAAITVTHVDTSTGARAPIEKIGEVLKKFPDT
                     IYIVDGVAASAGEREYVDDMNIDILFTGSQKAFGVAPGLAIVWAGKKALARRKSLGTI
                     PEYFIDFEKWIPIMNDPSKYYATPAVNMVWALKESLRLIKEEGLEERYERHTKVSNAF
                     NDSLEAMGFKLLAKKEYRCNTLSTVLYPEGINDAQFRKVLAEEGAVVAGGLAAYAGKM
                     FRIGHMGNIDDHTVYSTIAAIERTLIRLGHKYEVGIGLKTLL"
     gene            complement(1196..2092)
                     /locus_tag="PKH42_10210"
     CDS             complement(1196..2092)
                     /locus_tag="PKH42_10210"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOA20849.1"
                     /translation="MAYFKIKGAIPPMITPFNENDEVDFEKFARNIQDWNNYDLAGYL
                     VLGSNSETVYLTEDEKLKLVELTVKYRKPGKLVIAGTGMESTRATIDLTNKAADLGAD
                     CALLVTPFYYGGKMNDEALINYYTDVADNVKIPLMIYNVTKFTHINISARAVGVLCKH
                     PNIIGMKDSSGNVPQLIEFKKAVGNAEFNLMVGTASAWYPALTLGVEAAIMALANCCP
                     GECVDVQKEFEAGNLEKAREIYERVFPVNAAVTGTFGIAGLKYACDILGFNGGYVRKP
                     LLQLKEDDKVKLKAILKDANLI"
BASE COUNT          600 a          473 c          295 g          750 t
ORIGIN      
        1 agcaatgttt ttaaacctat tcctacttca tacttgtgtc ctaatctaat caatgttctt
       61 tcgatagctg caattgtcga ataaaccgtg tggtcatcaa tattacccat atgtcctatt
      121 ctaaacattt tgcctgcata tgcagcaagt cctcctgcta ccactgcgcc ttcttcagcc
      181 agtacctttc tgaactgcgc atcatttatt ccttcaggat acaaaactgt tgaaagtgta
      241 ttgcatctgt attctttttt tgccaataac ttaaatccca ttgcttctaa agaatcgttg
      301 aaagcattgg atacttttgt atgtctttca tatctttctt caagtccttc ttcttttata
      361 agtcttaatg attctttaag agcccaaacc atatttacgg caggagtagc ataatatttt
      421 gacgggtcgt tcattatcgg tatccatttt tcaaaatcaa tgaaatattc agggatagta
      481 cctaaggatt ttcttcttgc aagggctttc tttcctgccc atactattgc taaacccggt
      541 gcaactccaa atgccttttg agagcctgta aacaagatat caatattcat atcatcaaca
      601 tattctcttt cacctgctga agctgcaact ccgtcaacta tgtatattgt gtcagggaat
      661 ttctttaaaa cttcacctat tttttcaatt ggtgcccgtg cacccgttga agtatccaca
      721 tgggttacgg taattgccgc ataatttttt tctttcaatt tttcttcgat agcttcagga
      781 gaaacaacat ctccccattc agcagacaat acatctacat taagaccttt cctgttacag
      841 atttcaataa atctgtcacc aaaaaatccg ttagatacta tcaatatatt atcatctctt
      901 tttgtaacat ttgcaactgc catttccata gccattgtac cgcttccggc tacaacaaag
      961 cattcaccgt caactctcca catttccttt aaatctacta ttaactcgct aaaatcctta
     1021 acaaaaacag gatctccaaa tgcaactgtt tctcttccca tttggtcatt tatagttctt
     1081 actgtgggag tgggaccggg aatcattact aatttcttgt ttttcatagt atcctcctat
     1141 actaaaattt tatttttacc tttgttatat aaagacactc tttaaagaag tttaattata
     1201 ttaaatttgc atcttttaag attgctttca gtttaacctt atcatcttcc tttaactgca
     1261 ataaaggttt cctgacatat ccgccgttaa atcctaatat atcgcaagca tatttcaaac
     1321 cggcgattcc aaaagttcct gtaacagctg cattaaccgg gaatactctt tcataaattt
     1381 ctcttgcttt ttcgaggtta cctgcctcaa attccttttg aacatctacg cattcacccg
     1441 gacagcagtt tgctaaagcc ataattgctg cctcaacgcc taaggtcaga gcagggtacc
     1501 aagcagatgc agtaccgacc atcaaattaa attcagcatt gccgacagct tttttgaatt
     1561 ctatcagctg tggaacattg cctgaactat ctttcattcc tataatgttt ggatgtttgc
     1621 acaagacacc tactgcacga gcagaaatat ttatatgagt aaacttagtc acattgtaaa
     1681 tcattagagg aatttttaca ttatcggcaa catccgtata ataattaatc aaagcttcat
     1741 cattcatttt ccctccataa taaaatggag tgacaagaag tgcacagtcg gcacctaaat
     1801 cagccgcctt attcgttaaa tcgatggtgg ctcttgttga ttccatccct gttcctgcaa
     1861 tcacaagttt acccggtttt ctatatttta ctgtgagctc tactaactta agcttttcat
     1921 cctctgttaa atatactgtt tcactgttag agccaagaac gagatagccg gctaaatcat
     1981 agttattcca atcctgaata tttcttgcaa atttttcaaa atcaacctca tcgttttcat
     2041 taaaaggagt tatcattgga ggtattgctc ccttaatctt aaaatatgcc atttttctct
     2101 cctattattt attttatt
//
LOCUS       DAOPUE010000194         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_57990_length_2072_cov_11.224095, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000194 DAOPUE010000000
VERSION     DAOPUE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57990_length_2072_cov_11.224095"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..196)
                     /locus_tag="PKH42_10215"
     CDS             complement(14..196)
                     /locus_tag="PKH42_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOA20850.1"
                     /translation="MEKNKKDKEKKQLLLRLSSSLWEELASWAEDDFRSINGQIEYLL
                     NECVKKRKKTSKKSAE"
     gene            complement(263..1264)
                     /locus_tag="PKH42_10220"
     CDS             complement(263..1264)
                     /locus_tag="PKH42_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOA20851.1"
                     /translation="MNKEKLITEEIVPSSMNGMFMLILNIVLMLGSILLFIISLSSLE
                     GALQVVMIVISILYFSVIGPVLFAGLKVLKPNEALVLTLFGKYYGTLKGEGFYFVNPF
                     VTSINPAGNAATSALLSIEGVSKDKKNPDLNINMKSKKISLKTMTLNNDRQKINDALG
                     NPITIGIVVIWRVVNTAKAVFNVDNYTEYLSIQCDSALRNIVRLYPYDTFGDDNEKTL
                     RGSSLEIANKLKEEIQEKVEVAGLEIIEARITHLAYAPEIAAAMLQRQQASAIIDARQ
                     MIVEGAVGMVEMALEKLSENNVVELDEERKAAMVSNLLVVLCGNKDAQPIVNSGSLY"
     gene            complement(1464..>2072)
                     /locus_tag="PKH42_10225"
     CDS             complement(1464..>2072)
                     /locus_tag="PKH42_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOA20852.1"
                     /translation="FISFIISIYMLSEKDDLLARSRRFIHAYFSDKKAKDILDVFEKA
                     NKIFKSFLGGKLLDSAIVGIMFVIAFALAKVPYAPLMGTLTGFFNIIPYFGPLIGSVP
                     VIIVSFFVNPTKALTALIIIIIIQQIDANILEPKIVGENVGVSPFWIITSVTVGGNLF
                     GIPGLILGVPVVVLLKTVLEGSMEMRLIEKDKEDIEKTNLRK"
BASE COUNT          591 a          429 c          293 g          759 t
ORIGIN      
        1 atacatttac tgcttattcg gcagattttt tgcttgtttt tttccttttc tttacacact
       61 cattgagcaa atattctatt tgaccgttta ttgagcgaaa atcatcctca gcccaacttg
      121 ctaattcctc ccaaagagat gatgataatc tcaataataa ttgtttcttt tctttgtcct
      181 ttttattttt ttctatgtcc gttttatttt tttctattat caccagctcc tttccatagt
      241 ttctcacact aatcacttac cgttaataaa gtgagcccga gtttactatg ggctgtgcat
      301 ctttgttacc gcaaagaaca actaacaaat tgctgaccat tgccgctttt ctttcttcat
      361 ctaattctac aacattattt tcactaagtt tttcaagtgc catttcaacc attccaacgg
      421 cgccttccac tatcatctgc cttgcatcga ttatagctga agcttgttgt ctttgaagca
      481 ttgctgcagc tatttcaggt gcatatgcca agtgagtgat tcttgcttca attatttcaa
      541 gacctgcaac ttctactttt tcctgaattt cctctttaag cttattggca atttccaaac
      601 tgctgcccct taatgttttt tcgttgtcat cgccgaatgt atcgtaaggg taaagtctca
      661 caatatttct taaggcagaa tcacattgaa ttgacaaata ttcggtgtag ttatccacgt
      721 tgaaaactgc ctttgctgta ttaactactc tccaaataac cactattcct atagtaatgg
      781 gatttcctaa ggcatcattt attttttgcc tgtcattgtt taatgtcatt gtttttaatg
      841 atattttttt gcttttcatg tttatattca aatcaggatt ttttttatct ttactaactc
      901 cttcaattga aagtagtgct gatgttgcag cattgccggc cggatttatg gaagttacga
      961 acggattaac aaaatagaat ccttcacctt ttagcgtacc gtaatatttt ccgaataatg
     1021 tgagaacaag tgcttcattc ggttttaaca cctttagtcc tgcaaacaaa acaggaccta
     1081 tgacggaaaa atataaaatg cttattacaa tcataacaac ttgcagtgca ccttccaaag
     1141 aacttaaact aataataaac agcaatatcg agcctaacat taatacaata ttaagaatca
     1201 gcataaacat gccattcata gaacttggta ctatttcttc agtaatcaat ttttctttat
     1261 tcatttttga tatttcctcc ttatttgata tttcatgata tcaatttgat atcattatta
     1321 tattatacca tttcgctggc gtcaatatat tttgtaaaaa ttatttatca taaattacta
     1381 aaaaaatatc gctacagctt tatgcgatat tttctgattt attctttatg acagtacccc
     1441 cgtccccttg tcacgtcccc ttgtcatttt cggaggttgg ttttttctat gtcttcctta
     1501 tccttttcta tcagacgcat ttccatggag ccctctaaga ctgtcttaag caggactaca
     1561 accggtaccc ctaatatcaa tcccggaatg ccaaaaagat ttcctccaac tgttacgctt
     1621 gtaataatcc aaaaagggct tactcccaca ttttctccta ctattttcgg ctccaatata
     1681 tttgcgtcta tctgctgtat gatgataata attatcagag cagtcaacgc tttggtagga
     1741 ttgacgaaaa atgatacaat aatcaccgga acagaaccta ttaagggacc gaagtaaggt
     1801 attatattaa aaaatcctgt cagagttccc atcaagggag cgtatggcac ctttgcaaga
     1861 gcaaatgcta ttacaaacat tattcccact atggcggaat ccagcaattt accgcctaaa
     1921 aaacttttaa atattttatt tgctttttca aagacatcaa gtatatcttt tgcttttttg
     1981 tcgctgaagt aggcgtgtat aaatctcctg cttcttgcca aaaggtcatc cttttcagac
     2041 aacatgtaga tactgattat aaagctgata aa
//
LOCUS       DAOPUE010000195         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_64140_length_1940_cov_11.198939, whole genome shotgun
            sequence.
ACCESSION   DAOPUE010000195 DAOPUE010000000
VERSION     DAOPUE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64140_length_1940_cov_11.198939"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /gene="ftsX"
                     /locus_tag="PKH42_10230"
     CDS             <1..762
                     /gene="ftsX"
                     /locus_tag="PKH42_10230"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOA20853.1"
                     /translation="NNLAMVAKETFDQIAVYIEEGTEDEQIEEMGRSFRQIDGVMGVA
                     YQTKDYALERVKEDWGEDAVYLQDLRNNPFPNTYIVQLSDVSKSDEVIAKIETFEGVD
                     EVRYYQEAVNTLIKMADFVKKFGAGIIIALLLISVFIISNTIKITVLSRQREIELMQY
                     IGATNGYVRGPFILEGIMLGLLGSVLAILLIMLGYNYTINYLAGRYVALISGMSGYLV
                     GVEMVLDDLIIIFLTIGIGIGILGSLISLKKFLSV"
     gene            773..1906
                     /locus_tag="PKH42_10235"
     CDS             773..1906
                     /locus_tag="PKH42_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOA20854.1"
                     /translation="MFRKCVVLILLIVTIISIFPVNAGGSIADLRYKQQQIENEIKEI
                     RQKADALKGEKKTIEAEIQILDSDLRALTLEIQKQEVQKEEMNMRIAESEQKIVDLID
                     EIDENNELLEERLRAMYINGTGGYLEVILKAENLIDALTRMDMIQLIVKSDVDLLKTI
                     NEQKTQVEELKVEQETRRHELTAIINDLNAKQDEVLIASRSKENYMVSLQDNIEEILR
                     QEAAMEEQSKQIERDILAAQRAVEYAGGELLYPLPGYYRITSPYGGRSHPITGVWSNH
                     GGTDIWAPYGTNILSSNDGVVIFAGYHYSYGNYIIVDHGGGIATLYAHCSKLLAWEGQ
                     AVTKGEAIAQIGSTGESTGNHLHYEVRVNGVRQNPMDYLKARN"
BASE COUNT          683 a          298 c          435 g          524 t
ORIGIN      
        1 aataatctgg caatggttgc caaagaaact ttcgaccaga ttgcagttta tatagaagaa
       61 ggcacggagg atgagcaaat agaggaaatg ggcagatcct tcagacaaat tgacggagtt
      121 atgggggtag cataccagac aaaggactat gctcttgaaa gggttaagga agactggggc
      181 gaagatgcag tttatcttca ggatttaaga aacaatcctt tccccaacac ctatattgta
      241 cagctttccg atgtaagcaa gtcggatgaa gtgattgcaa agattgaaac atttgaaggc
      301 gttgatgaag taaggtatta tcaagaagca gtgaacactc ttataaaaat ggccgatttt
      361 gtaaaaaaat tcggagcagg gataataata gcccttttac ttataagtgt attcattatt
      421 tcaaacacaa ttaaaattac cgtcttaagc agacaaagag aaattgagct tatgcagtat
      481 attggagcta ccaatggata tgtaagggga ccctttatac tggaaggtat catgctggga
      541 cttttaggct ctgttttggc aattttactt attatgcttg gatataatta taccatcaat
      601 taccttgccg gccgctatgt ggcacttata tcaggaatgt caggatattt ggtaggcgtt
      661 gaaatggtat tagatgacct tataataata tttttaacta taggaatagg tattggaata
      721 ttaggaagct taatttcatt gaagaaattt ttaagtgttt aaggagtgtg ccatgtttag
      781 aaaatgtgta gttttgattt tattaatagt aacgattatt agtatttttc ctgttaatgc
      841 agggggaagc attgctgatt taaggtacaa gcagcagcaa attgaaaatg aaatcaagga
      901 aatccgccaa aaggcagatg ctcttaaagg cgaaaagaaa acaattgaag cggaaattca
      961 aattctggac agcgacctca gagctttaac cttggaaatt caaaaacaag aagttcaaaa
     1021 agaagaaatg aatatgcgca tagctgagtc agaacaaaaa attgttgacc ttattgatga
     1081 aatagatgag aataacgaac tcttggaaga acgcttaagg gctatgtata taaacggaac
     1141 aggcggatac ctggaagtaa tactaaaagc tgaaaatctt attgatgcat tgaccagaat
     1201 ggatatgatt caattaattg ttaaaagcga tgtggaccta ttaaagacca tcaatgaaca
     1261 aaaaacacaa gttgaagaat tgaaagttga acaggaaacc agaagacatg agctgactgc
     1321 cataataaac gatttgaatg ctaagcaaga tgaagtgtta atagcttctc gttcaaaaga
     1381 aaactatatg gtaagcttgc aggataatat tgaagaaatt ctaaggcagg aagctgccat
     1441 ggaagagcag tcaaaacaaa tagaaagaga catattagcg gcccagcgtg ctgttgaata
     1501 tgcaggaggg gaattgcttt atcccttacc cggatactac aggattactt ctccatatgg
     1561 gggaaggagt catcctataa caggtgtatg gtcaaatcac ggaggaactg atatttgggc
     1621 accctacggt acgaatatcc tttcatcaaa tgacggagtt gtaatatttg caggttatca
     1681 ttattcctac ggcaattaca taattgttga ccatggagga ggaatagcaa cactttacgc
     1741 ccactgctca aaattgttgg cttgggaggg acaagccgta acaaaaggag aagccatagc
     1801 acagattggg tctaccggag aatctaccgg aaaccatctt cattacgaag tgagagttaa
     1861 cggggttaga caaaacccca tggactattt gaaagcacgc aattaaattt agcaattttt
     1921 agggacggtt cttttcattc
//
LOCUS       DAOPUE010000196         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_1426
            NODE_76713_length_1729_cov_8.729391, whole genome shotgun sequence.
ACCESSION   DAOPUE010000196 DAOPUE010000000
VERSION     DAOPUE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854631
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 00:44:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,048
            CDSs (total)                      :: 2,032
            Genes (coding)                    :: 2,021
            CDSs (with protein)               :: 2,021
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 2 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76713_length_1729_cov_8.729391"
                     /isolate="AMR_MDS_1426"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PKH42_10240"
     CDS             <1..1511
                     /locus_tag="PKH42_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOA20855.1"
                     /translation="DGYMDILKGQSYIDSKPVETFPDTAAKAEELKAILMESVAETDE
                     ELLEKYFEGEEFTEEEIQTGLKKAVLEGELVPVIVGSAIKGLGVDMLMNIVVEYLPNA
                     LDAKSLKGSPSDSLSAFIFKTIVDPFVGKISLYKVMSGKMKKDTDVYNANTGDTERIG
                     SVFSLRGKEQLEVSEVEAGDIGATSKLQYFKTGDTISLKTAPVSYDKIDFPKPCYFMA
                     VKGKTKDSDEKIGTGLQRLNEEDPTFSIERNIETKELIVGGQGNIQLDVLASKLKNNY
                     KVDVELSPPKVAYRETIKGKSDVQGKHKKQSGGAGQYGDVRIRFEPSQEEFEFTEEIF
                     GGSVPKNYIPAVEKGLRESLEKGVLAGCKVVNVKATLYDGSYHEVDSNEMAFKIAASL
                     AFKKGMLEAKPILLEPIAKVEINIPDEYLGDIMGDMNKRRGRILGMEQQADGSQLVKA
                     EAPMAEMYTYAIELKSMTQARGTFEVEFLRYEEMPNNLAEKVIAEYKAANEN"
BASE COUNT          646 a          254 c          362 g          467 t
ORIGIN      
        1 ttgacggata tatggatata ttgaaaggac aaagctatat agattcaaaa ccggttgaaa
       61 cattcccgga cacagcggca aaggcggaag aattaaaagc aattctaatg gaatccgtag
      121 ccgaaacaga tgaagaatta cttgaaaaat actttgaagg cgaagaattt actgaagaag
      181 aaattcaaac cggattaaaa aaagcagtat tagaaggtga attggttcct gtaatagttg
      241 gttccgcaat aaaaggatta ggcgttgaca tgcttatgaa tatagtagta gagtatttgc
      301 ctaacgcttt agatgcaaaa tcacttaaag gtagtccttc agattcatta tctgcattta
      361 tatttaagac catagttgac cccttcgtag gaaaaatttc attatataag gtaatgtcag
      421 gaaagatgaa aaaagatacg gatgtttata atgcaaacac cggtgatact gagagaatag
      481 gcagcgtatt ttccttaaga ggaaaagagc aattagaagt ttctgaagtt gaagcaggtg
      541 atataggtgc aacttccaag cttcagtatt ttaaaaccgg tgatactatt tcattaaaga
      601 ctgctccggt atcttatgat aaaattgatt ttccgaaacc ttgctacttt atggcagtta
      661 aagggaagac aaaagacagt gacgaaaaaa tcggcacggg acttcaaaga cttaacgaag
      721 aagacccgac gttctcaata gaaagaaata ttgaaactaa agagcttata gtaggcggac
      781 agggaaacat tcagttggat gtacttgcca gcaagcttaa gaataactac aaggttgatg
      841 ttgaactcag tccaccgaag gttgcataca gagaaacaat taaaggcaaa tcagatgttc
      901 agggaaaaca caaaaaacaa tcaggcggcg caggccagta tggagatgtt cgtatcagat
      961 ttgagccatc tcaggaagaa tttgaattta ctgaagaaat tttcggagga tcagtaccga
     1021 aaaactatat cccggcagta gaaaagggac ttagagaatc acttgaaaaa ggtgtattgg
     1081 caggatgtaa agttgttaat gttaaagcaa cattgtatga cggctcttac cacgaagttg
     1141 actcaaacga aatggctttt aaaattgcag catcattagc attcaaaaaa ggtatgctgg
     1201 aagcaaaacc tatattgctt gagcccattg caaaggttga aatcaatatt cccgatgagt
     1261 acttgggaga tataatgggt gatatgaaca agagaagagg aagaattctc ggtatggaac
     1321 agcaagcgga cggaagccag ctggtaaagg cagaagctcc aatggcagaa atgtacacat
     1381 atgcaatcga gcttaagtcc atgactcaag caagaggtac ttttgaagtc gaattcttaa
     1441 gatatgaaga aatgccgaac aacttagctg aaaaagttat agctgaatac aaggcagcga
     1501 atgaaaacta aagacatgta cgactataat aaagttatta aataataaaa agttgttgta
     1561 aaaaatcact taaaagattt attattatct tttaagtgat ttgtttttct tactaattag
     1621 cttaatttga agattttata ataaattatg ttcataatat ttgcaataag ccaaatttaa
     1681 cgagtaaaat ctattcttat aaattttttc ccgtttggta attttcttt
//
